LOCUS       DBMDTI010000001        54638 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_246_length_54638_cov_4.320296, whole genome shotgun sequence.
ACCESSION   DBMDTI010000001 DBMDTI010000000
VERSION     DBMDTI010000001.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 54638)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 54638)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54638
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_246_length_54638_cov_4.320296"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /gene="recQ"
                     /locus_tag="ACZPB6_00005"
     CDS             complement(<1..1763)
                     /gene="recQ"
                     /locus_tag="ACZPB6_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HIR1182895.1"
                     /translation="MRRFNTPIEVLNQVFGYESFRGFQEEVVETVVSGRDALVLMPTG
                     GGKSLCYQIPALLRDGVAVVVSPLIALMQDQVDALDEVGVKAAYLNSSLTPEEASRVK
                     DELLSGRLDLLYVAPERLMMSSMLSLLDRVNVSLFAIDEAHCVSVWGHDFRPEYGELE
                     LLRTRYPQVPRIALTATADEKTRAEIVGKLLNDAREFVASFDRPNIFYRIVDKRNIKE
                     QLLNFIKYEHSGDSGIVYCLARRTTEEIAQYLSGCGIEALPYHAGLPAEERARNQAMF
                     LREDGIVMVATIAFGMGIDKPNVRFVAHVDMPKSIEGYFQETGRAGRDGEPADAWMAY
                     GLNDVVNQRFFIDASDADEMHKQLCTQKLDAMLGLAEAPDCRRQRLLAYFGEKSEPCG
                     HCDNCLEPPDLLDMTVPAKKFISCIYRIREASGVCFGAQHVIDVLMGNMTDRVARFSH
                     DKLSTWSIGQDLSAAEWRILLRQLVARHVVWVDPTARNALSLGEAARDLLRGNMKISV
                     RKRVAETRRKRREHSQTEALLQEFSKRDRSLYEALRQWRSDEAKSLGKPPYVIFKDAT
                     LLEIVRERPTSPEALRRISGVG"
     gene            complement(1858..3087)
                     /gene="sstT"
                     /locus_tag="ACZPB6_00010"
     CDS             complement(1858..3087)
                     /gene="sstT"
                     /locus_tag="ACZPB6_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HIR1182896.1"
                     /translation="MKRVFNFAMPFLQASLVKQILVGLVLGIALAYAAPAAAVSCGFL
                     GTVFVTALKAVAPILVFVLVMSSIANQNLSGENLHIKPIIVLYLIGTFSAAIVAVVAS
                     FMFPTKLILTATDAVATTPGGIGEVLGNVILNVVDNPVHAISSGNYIGILAWAIAMGV
                     VLRHGTVATREVLNDAAKGVEFVVRLVIRFAPVGIFGLVANTFATTGAEVFIGYIQLL
                     VVLLGSMFFVALVINPFLVWFCTHKNPFPVTLMTLRESGVAAFFTRSSAANIPVNLEI
                     CRRLGLPESTYSISIPVGATINMAGAAITITVLTLSAAYSLNIPVDLGTAVFLSFVAS
                     ICACGASGVPGGSLMLIPLACGLFGIDQNTAMQVVAVGFIIGVLQDSCETALNSSSDA
                     LFTIAACQRAERLERGE"
     gene            3403..4605
                     /locus_tag="ACZPB6_00015"
     CDS             3403..4605
                     /locus_tag="ACZPB6_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1182897.1"
                     /translation="MMRKPAIGFVLACVFLDALGIGLIVPVLPRLIGSLADTRDLQTS
                     WYGAIMVSYGLMQFFFAPILGALSDRIGRRPVLLTGILGLSIMMIVPAVSQSLPFILL
                     SRLVGGMMSSNIVVAQAYIADVTPSHQRIASFGKIGAIFGIAFVLGPAVGGVLGQTDP
                     KIPFFVASAICALNFLYGFFILPESLRARSTAPLSLGRLNPFSAIWSLAGNLSLRPLL
                     VVITLFTLAQSLMQCTWALYTEYRYAWTPLMIGMSIFALGLSISLTQGFILPRLVQKL
                     APGRIIVTGLLIGLSSMLVIALSPSGAVSLAMVCLFAVMGIVGPTIQGLISRRCEAST
                     QGVNMGAVSSLNSFTGAISPLIGTPLLMITADHHMHPVLAGAPYLATVLLIACALALT
                     LKTNVGGD"
     gene            complement(4714..6339)
                     /locus_tag="ACZPB6_00020"
     CDS             complement(4714..6339)
                     /locus_tag="ACZPB6_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR1182898.1"
                     /translation="MSSKPFAITLDVGTSLANRTGSWRTLRPVYVNRLPPCNAKCPAG
                     EQCQAWLYHAESGDYRHAWEKIVEDNPFPACMGRVCYHTCESACNRGELDESVGINAV
                     ERFLGDHALENGWSFKKPETKSGKRVLVVGAGPAGLSAAYHLARMGHEVCVRDGAKAA
                     GGMMRYGIPKYRLPREVLDKEIARIAELGVKFELSREVTDLAAEMKEGGFDAVFLGVG
                     ASLARRAYIPAGDAAHVLDAVTVLRSMEGEEQPLLGRRVVVYGGGNTAIDVARTAKRL
                     GAEPLVVYRRTRERAPAHQFEIEEAIEEGVHMKWLSTIAHADEGEIRIEKMKLDESGR
                     PVPTGEFETVSADSVVLALGQETNLKFLEGIDDLQMENGSVVVDGSMMTGCPGIFAGG
                     DMVPGERNVTVAIGHGKRAARSIDAWLAGRPMPAAEERQPATFGRLNTWYYADAPKSV
                     RPQLDLVRRQTTFDEVVQGLTEQTALFEARRCMSCGNCFECDNCYGVCPDNAIIKLGP
                     GKKFRINYEYCKGCGICANECPCGAIDMVPESI"
     gene            complement(6362..7420)
                     /locus_tag="ACZPB6_00025"
     CDS             complement(6362..7420)
                     /locus_tag="ACZPB6_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR1182899.1"
                     /translation="MSEVVHFYQTGTFTVGNRLVTPEERAGQASMERTNALTSGHRAC
                     QGCGEALGARYAVDAAMRAVNGRLAAANATGCLEVFSTPYPETSWQLPWFHSLFGNTA
                     AVATGMAAAFKVKAKKSGGAETRVIAMGGDGGTTDIGFGCLSGMFERNDDVLYICYDN
                     EAYMNTGVQRSSATPPAARTATTQVAGDEPGNAWGQGKDVPLIAMAHGIPYVATATVA
                     DLRDLERKVTKAMSVRGARYIHILVPCPLGWGHGSGLTVQMARMAQQTGIFPVFEAEY
                     GEVTGVSKIRRKIPVEQYLKPQKRFAHLFGKNADLTVIARIQAMADRNIKDYGLIDEE
                     EVETHPTAVQGDERAHHF"
     gene            complement(7424..8689)
                     /locus_tag="ACZPB6_00030"
     CDS             complement(7424..8689)
                     /locus_tag="ACZPB6_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1182900.1"
                     /translation="MLKQLEGSQGVAQAVALCRPEVVCAYPISPQTHIVEALGALCRK
                     GVLKDCEYINVESEFAAMSVAIGASVAGARAYTATASQGLLFMVEAVYNASGLGLPIV
                     MTVANRAIGAPINIWNDHSDSMSQRDSGWIQLFAETNQEAVDLHIQAFRIAEELSLPV
                     MVCMDGFVLTHAFERMDIPSQEDVDAFLPPYEPRQVLDPDHPYSIGAMVGPEAFTEVR
                     WLAHKRMLDALPAIEKASADFAERFGRKSGGLLDTYRMEDAEVCVLAMGSILGTIKDT
                     VDEMREAGVRIGVLCLKSYRPFPDEALREAVKSVKTLVVLERMVSAGSGGALYLDVLK
                     AVRREPVELRSVIAGLGGRAVTKASLRAVFESACNGTLPDETVFLDLDRTLVEHQLAR
                     EAEQRHTGPVAESLNREVNERKILQGEEI"
     gene            complement(8682..9260)
                     /locus_tag="ACZPB6_00035"
     CDS             complement(8682..9260)
                     /locus_tag="ACZPB6_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR1182901.1"
                     /translation="MFQIRIHGRGGQGVVTAAEMLALAGFMEGHHAQAFPSFGSERTG
                     APVVAFARLASSEIRLREPVLEPDVVLVQDKTLLESVDVFKGLREDGYVLINSSETPE
                     ALGLEDLKKRLPEGHFLCVPATAYALEKIGRPLPGAAMLAGMAAMTGSMKLESVIAAY
                     NEKFSGRVAQANAEVAKLAFDSVKSMLGGVHA"
     gene            9556..10050
                     /locus_tag="ACZPB6_00040"
     CDS             9556..10050
                     /locus_tag="ACZPB6_00040"
                     /inference="COORDINATES: protein motif:HMM:NF023363.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HIR1182902.1"
                     /translation="MLAGCSSLLPQGTPGVSGQDGRSVSHWRDAISSASRISTRTARG
                     RMHFVCSYDAKGFYWRFSHPSGGLYRGNTKVGTLNSDWSITASDGTTLKMSVLTNGPR
                     RSAEDLTDAVFKASAPKKGTFAGVRYVERTNTRGGMPLTKCSASQQGQRLSRPFEADY
                     TFWR"
     gene            10160..10654
                     /locus_tag="ACZPB6_00045"
     CDS             10160..10654
                     /locus_tag="ACZPB6_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR1182903.1"
                     /translation="MVPAATTVGGYLQSLPNDRRVAMARVCSMIRRSARGVRESMRFG
                     LAFYELDGPLFAVESHEKSLVLYLAERDVVKDFKDKIKNIDIEHCRVEFPDLNCLSLD
                     VVEKLVRASLVCRRARKASAEEPPSQADLVALWGLTEKDVEAAPPIVRITINREDEEA
                     PAAK"
     gene            complement(10734..11648)
                     /locus_tag="ACZPB6_00050"
     CDS             complement(10734..11648)
                     /locus_tag="ACZPB6_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1182904.1"
                     /translation="MGCPEELLFDLVEGEEHREVALADGALSYAVASCRSGPCAGTIV
                     LIHGVGSNASRWEEFTEQTPLREGWRIIRLDLRGHGASESREKATLEIHAADLMRVLD
                     DAGIEKAVLVGHSLGAQIAMRAAVLYPDRIQGMVLMDPLVTSALTPSALAHRRKYPLL
                     VTVEFLARMLNALGIRRRMPHYSLRAHDRKVREMLARGGDALQAFIDEYSSPLKDLGH
                     IHTACYMRDILEVGRETPDLSGVGFPVLVIGASSGTFTDAGRMQEWVRSLRDGSWAVV
                     NCFHWPLTECPEEVSRIIEEWIGREFRG"
     gene            complement(11651..12880)
                     /locus_tag="ACZPB6_00055"
     CDS             complement(11651..12880)
                     /locus_tag="ACZPB6_00055"
                     /inference="COORDINATES: protein motif:HMM:NF025885.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR1182905.1"
                     /translation="MSVSSELKTVLDAFDDPRLLVRADHTVAYANRAFVRRYGRQDFA
                     GRSCYELLFHKSSCCAECGERCPLESAAVTGASETVLRRELGPTGVRYLELCSTPLKG
                     ADGRAVLFMESIADRNGTQEPLNLRGVVAESPATKAVLEKISLVTALDVPVLFIGPSG
                     SGKREFARLLHENSRRAAHSFLCVDCRGLTPAKLSRELQLTAPFGLSGGTLYLMGIEE
                     LSADMQGAVLRMLETGSWVDTEGASCERADIRLVCGTRKSLAELLEADVLREELYYRL
                     CVCPIRVPGLEERAEDLPEIVRLTLQSLKAKGLELRLAPGVCEALSRRTWRGHVRELQ
                     AVLERAAIFSRGEIVGVETLFDDEPVPAEGRDASEEARLRALIRGWKGSRADLAAHLG
                     VSERTVYRWIERSRQEK"
     gene            13035..13238
                     /gene="cydP"
                     /locus_tag="ACZPB6_00060"
     CDS             13035..13238
                     /gene="cydP"
                     /locus_tag="ACZPB6_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase putative small subunit CydP"
                     /protein_id="HIR1182906.1"
                     /translation="MKTNNVSPGKSKLGREIAVVIACKLLFIFGLWYFFFSPEHRPEV
                     NADVVGNVVLGAADAPAKANPTK"
     gene            13254..14804
                     /locus_tag="ACZPB6_00065"
     CDS             13254..14804
                     /locus_tag="ACZPB6_00065"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR1182907.1"
                     /translation="MIPSDLVDLSRLQFAVTAMYHFIFVPLTLGLSFMLVAMEVTYVL
                     TGKEIYKDMTRFWGKLFGINFALGVATGITMEFQFGTNWAYYSHYVGDIFGAPLAIEG
                     LMAFFLESTFIGLFFFGWDRLSKGKHLAATILMAVGSNLSALWILVANAWMMNPVGAE
                     FNFQTMRMEMVDFWAVLTNEWAQAKFVHTVAGGYMTGAIFVMAISAYYMLRRQDLQFA
                     RSSFRLAAVFGMIATLMTIQMGDESGYLVTKDQPTKIAAMEAIWETHEAPAPLTLFAI
                     PDEAAQTNHAQIDVPWLFGLMGTRSLSTEIPGIKELTAQAEQRIVNGQKAIGVLEQLR
                     KDPANEQLREQFLAVRDDLGFGLLLKRYTNDVNAATPEMIKQAAADTIPSVTPMFWSF
                     RIMVGSGLAMALVFVLGLFYSFRNEIAEKAWYLRLAVLALPLPWIASEAGWFVAEYGR
                     QPWTIYNVLPTHLSVSALTAGDVAGSLLGFGVLYTALLVVEMWLMIRISRRGPSALGT
                     GRYHFERN"
     gene            14819..15958
                     /gene="cydB"
                     /locus_tag="ACZPB6_00070"
     CDS             14819..15958
                     /gene="cydB"
                     /locus_tag="ACZPB6_00070"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013693155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HIR1182908.1"
                     /translation="MIDYEILKVIWWLFIGVLLVGFAVMDGHDMGVGSLLPFLGKDDN
                     ERRVMINSVAPHWDGNQVWLITGGGAMFAAWPMMYATAFSGFYWAMLVLLMALIFRPV
                     AFDFRSKVAATKWRTTWDWLLFLGSAIPPVICGVAFGNLLQGVPFHIDETMRPIYTGS
                     FFGLLNPFGLLCGVLSLVMIVAHGGNYLTLRTEGALQARARNVAFIAGIVSAVLFVIG
                     GLWVWAGIDGYVAVAGLDPAGPANPLAKTVEIQAGAWFANFASVPVLWLVPALGVIGA
                     LGGAVMVRANKPGFAFVLSAASLLGIILTPLLAMFPFVMPSSTMPNAGLTLWDCTSSQ
                     LTLEIMLFVTLIFLPLVLLYTGWAYKVMSGKLNSEYIEKNSHSLY"
     gene            16101..16391
                     /locus_tag="ACZPB6_00075"
     CDS             16101..16391
                     /locus_tag="ACZPB6_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon YbgE family protein"
                     /protein_id="HIR1182909.1"
                     /translation="MSTDELKKTPGANGFIRTLSILAAAAVVALVVIYPRAVATDGAS
                     VPHGWFVLMMMGMSICWICGFGFTPENRWLRRLLSLPVGWTMLLIGAWQVFS"
     gene            16406..16789
                     /locus_tag="ACZPB6_00080"
     CDS             16406..16789
                     /locus_tag="ACZPB6_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HIR1182910.1"
                     /translation="MSNLNSLYARLGGEEGVIRLVKAYIEALKTLPGAQRLRSLYPED
                     LSKYELRMTEFLSTWLGGPALYQERHGMPMLRESHRSFAIDGEARDEWMLCMRTALRE
                     TVSDEELRLYLEGAFWRMADSLRTK"
     gene            complement(16836..17399)
                     /locus_tag="ACZPB6_00085"
     CDS             complement(16836..17399)
                     /locus_tag="ACZPB6_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HIR1182911.1"
                     /translation="MALGATVYKATIDVSDLDRDSYGNYVLTIARHPSETEERLMLRV
                     LAFCRLAGERLEFGRGLSTEGEPALWEIDDTGAIVQWIEVGCPDVKQVRKAAGRSEHV
                     QIVAYGEDRVEPWWEGNKGDFAKIGKLTVVSVPDDQVTALASMASRNMKLAVTIQDGI
                     LWISDDRDSLQIEVTVLQTEHERYSDF"
     gene            complement(17436..17840)
                     /locus_tag="ACZPB6_00090"
     CDS             complement(17436..17840)
                     /locus_tag="ACZPB6_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 family protein"
                     /protein_id="HIR1182912.1"
                     /translation="MEPRVYEPSDGTRIVAHVVYGLHTVSILLGLMTGASIAGAFIFS
                     WPSILAVILNYIFRGDAEGTYLESHFAWQIRTFWLAALWLVVTGLFGWALLFFGIGFF
                     ILIIGFLVLGVWIAYRIGYGWLRLGRHEALPL"
     gene            17892..18476
                     /locus_tag="ACZPB6_00095"
     CDS             17892..18476
                     /locus_tag="ACZPB6_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1182913.1"
                     /translation="MNFISDWALPILFLLASADRTAAILMAVLFATARITGPAEAAGA
                     VALGSACLTLLLWRIGLRHKKREEVPPPSACDYTIGQWLRKHGPAGAAARVVPAVGWR
                     FSGTVALASQSGGRPAVCLYAVLFTVLWVLFWILVLMPAAERLMAATTTLTHVRMTLP
                     GLMLLAFTAAVVLRTALCRLHQRYQEKKHENSCR"
     gene            18457..19113
                     /locus_tag="ACZPB6_00100"
     CDS             18457..19113
                     /locus_tag="ACZPB6_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1182914.1"
                     /translation="MRILVAEDDDMIGESLVAFLESEDYAVDWVRDGQSALLAMKTTP
                     FSLVILDLGLPGKDGLQVLKEVRARRDPTPVLVTTARDTVDDRVKGLDSGADDYLVKP
                     YELDELSARVRALLRRSAGRADPIIERGALRISPATREVTFNGEPVILSSKEYALLAA
                     LAERQGVVWSRSQLEEKLYNWDNTVGSNAIEVHIHHLRKKLSDNAIKTVRGVGYLLET
                     "
     gene            19117..20463
                     /locus_tag="ACZPB6_00105"
     CDS             19117..20463
                     /locus_tag="ACZPB6_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1182915.1"
                     /translation="MHSIRRKLLITLIGALLAAGFTTAAATFFSAQTEFNKFLDTHLK
                     ETAESLAQSVTHSFPLADPDHLTIIGDAQSYHIVVQVYDSANNTVWQREDQPYLPLPD
                     APGFARAKLNGVNWRTYSTPAGPLIITVGQDTAVRTELAASSAFRVLQPLCLLLPFIA
                     IAVWIVVGSGLAPLEKTARSVARRSPTSLEPISTKGLPLELAGLVSAINQLLDRLRES
                     LSAQQRFASDAAHELRTPLTALKLQVQLAQRAKTDEAREKSLMRLNEGINRATRLVQQ
                     LLTLARLDPDSTKHPASTIKLDSLAHSVCEDMTPIASQKSITVTAVTKPASTEGLEDA
                     VRLMMTNLTDNAVRYTPEGGRIEIRTRTDADTDGAVIEICDNGPGIAPEERERVFDRF
                     YRALGTKTSGTGLGLAIVKRIVDIHHGTIAIDDGLDGRGTTFRIRLPKLGAAAAAV"
     gene            20622..22046
                     /locus_tag="ACZPB6_00110"
     CDS             20622..22046
                     /locus_tag="ACZPB6_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR1182916.1"
                     /translation="MFASKIARSATLSLAVAAVISSIAVPATAGVTIFGGSEDKQQVQ
                     LPDFVKLVEDNGPGVVNIQMIRNARTVENAGIPGLDPRGAEIFRRFGIPFDFGPQEIP
                     EQRGTGSGFIVSSDGIIMTNAHVVEGADELIVRLTDKREFKGKVLGSDKQTDIAVIKI
                     EAKDLPVLKIGDSSKLKVGEWVAAIGSPFGLDNTVTAGIVSALSRNLPSDQYVPFIQT
                     DVAVNPGNSGGPLFNMQGEVVGINSQIFSTSGGFMGLSFAIPIDLAMQIKDQLVKDGR
                     VTRGYVGVFIQEINQELADSLGLKTPEGALVTKTEKDSPAEKAGLRERDVILALNGKK
                     VTSSVTLPSLVSTIRPGTEVTMTVFRDGKEQEIKVTVGSNKATEEKQATAANETRLGV
                     TTRPLTKEEEKKAETTGLLVTQASGPAAKAGIRAGDILVSADGKLLKSRDDLLEQCKD
                     NKVLLLVQRNGGRIFIPVKLQAKK"
     gene            complement(22179..22499)
                     /locus_tag="ACZPB6_00115"
                     /pseudo
     CDS             complement(22179..22499)
                     /locus_tag="ACZPB6_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019984.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            complement(22899..24203)
                     /locus_tag="ACZPB6_00120"
     CDS             complement(22899..24203)
                     /locus_tag="ACZPB6_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR1182917.1"
                     /translation="MSAASANHKTPIYKVLYFQVICAIVIGILLGFLAPEIGAQMKPF
                     GDGFIRLIKMIIAPVIFCTVVTGIAGMEDMKKVGKTGGLALLYFEVVSTIALIIGLII
                     VNTVQPGVGMHVDPNTLDQAAVSAYTGPGKMQTTTEFLMNVIPNTVVGAFANGDILQV
                     LFFSILFGFALHKFGGRGTLIFDVIEKSAHVLFQIVGMIMRVAPVGAFGAMAFTIGKY
                     GIASLLPLAKLMGTFYLTCLLFIFGVLGTICRIHGFSIMRYISYIKEELLIVLGTSSS
                     ESVLPRMMTKMELAGVSRSVVGLVIPTGYSFNLDGTAIYLTMAAVFIAQACDVSMTLT
                     QQLTLLAVLLLTSKGAAGVTGSGFIVLAATLSAVGHVPVAGLALILGIDRFMSEARAL
                     TNMIGNGIACVVVGHWCREVDNDKLKVMMHSKKKYHENEDAS"
     gene            24553..25200
                     /locus_tag="ACZPB6_00125"
     CDS             24553..25200
                     /locus_tag="ACZPB6_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family NAAT transporter"
                     /protein_id="HIR1182918.1"
                     /translation="MLEAIHFQYFLGGLVSLLVITNPLSKLPLFVSLTQGMSEERRRQ
                     QANWAGIYAGIIMAVSLVGGNMLLAFFGISYGAMRISGGLVVAAIGYQMLFGGSSPNK
                     APIVRRDKDDYAFFPIAMPGIAGPGTIAVVIGFSTEIAELSNYADIAIAFGWTVLAII
                     FTAMASWAVMRYSDILTRRLGPSGTLVLGKLMGFLLICIGVQFIGSGIRTFMAGG"
     gene            complement(25286..27118)
                     /gene="glmS"
                     /locus_tag="ACZPB6_00130"
     CDS             complement(25286..27118)
                     /gene="glmS"
                     /locus_tag="ACZPB6_00130"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR1182919.1"
                     /translation="MCGIVAAASHRDVVPMLLEGLRRLEYRGYDSCGVATLDQGVLKR
                     ARTVSRVHELVERVAEDGVKGTVGIAHTRWATHGAPEVCNAHPHISRNRIALVHNGII
                     ENHEEIRRELQAKGYEFTSQTDTEVVAHLINDNDDGDLLKAVMTAVTRLRGSYAIAVI
                     AADEPDRVIAARQGSPMVVGVGDGENFIASDAMAVGGTTDRIVYVHEGDVVDVRPDSW
                     QVYERTEAGEFIPADREVKVVHAYAGAVELGPYRHFMQKEIFEQPRAVGDTLEGVAAF
                     SPTLFGAEAEGIFRNTQRLLFLACGTSYYSALTAKYWIEEIAGIPCDVEIASEYRYRV
                     SVPDPRTLVVTISQSGETADTLAALRHARDELGMSQTLTICNVATSAMVNESRLAYIT
                     RAGVEIGVASTKAFTTQLTALYLLACTLAKLNGRLTKEDEETELKRLRHLPAAMSAVL
                     ALEPQIIAWAESFAAKQHALFLGRGVHYPIALEGALKLKEISYIHAEAYPAGELKHGP
                     LALVDREMPVVTVAPNDRLLEKLKSNMQEVRARGGELYVCADGDSVIESSEGMHVIRM
                     PENYGRLSPILHVVPLQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            complement(27176..28537)
                     /gene="glmU"
                     /locus_tag="ACZPB6_00135"
     CDS             complement(27176..28537)
                     /gene="glmU"
                     /locus_tag="ACZPB6_00135"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR1182920.1"
                     /translation="MNIVILAAGMGKRMHSKLPKVLQPLAGRPMLDHVLDAVEGFAAV
                     KTVVVGHCAEEVQKHYEGRTDLQFALQMPQQGTGHALMQAWPVLDRNEPQTLVCLGDV
                     PLLSAETARRMLEEAEKSEFVLLTIEMDNPKGYGRIVRNAEGVVTAIVEEKDATDEQR
                     LIREVNTGIMVLPTARLDAWLDALKNDNAQKEYYLTDLVGFAAADGVRISAVHPDHAY
                     EVEGVNSKVQLAALERTWQRVQADRLLEAGVTLIDPDRIDIRGELVCGQDVEIDVGCV
                     FEGRVVLGNGVRVGAYSVIRNTTVEDDVEILPFCHFEGAAVGRDSCIGPYSRLRPGAE
                     LTGNNHIGNFVEVKKSVIGQDSKVNHLTYIGDADVGARVNVGAGTITCNYDGVNKFRT
                     VIGDDAFIGSDTQLIAPVEVGAGATIGAGTTLTRNAPAGKLTLSRARQMTIEGWTRPV
                     KKQ"
     gene            complement(28629..30134)
                     /locus_tag="ACZPB6_00140"
     CDS             complement(28629..30134)
                     /locus_tag="ACZPB6_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high affinity choline transporter 1"
                     /protein_id="HIR1182921.1"
                     /translation="MALIGFVMLYMLATIAIGLYAATRVSSTSDFALAGRSLPLPMVI
                     TATFATWFGSETILGLPGRFIESGIAGVIEEPFGSGMALILVGMFFAQKLYKLNILTI
                     GDYYRERYGRGVEVLCTLFIVLSYLGWVGAQVAALGLVINLITEGYISVSGGMVIGTV
                     VVLAYTMYGGMWSVAVTDFVQMIIIVLGLVAILFFASDLAGGFGKVIDYAHERDLFNA
                     LPEPTATGWLFWISSAITMMIGSIPQQDVFQRVMSARNVQTAVAGPVIGGCVYILFAF
                     VPMFIVIAAILVMPSSAQDMLATDPQRLLPTLVRDHMPLWLRVLFFGAVLSAVMSTAS
                     ATMLAPSTAFVSNILTHFVRLNDRNMLRAMRWTVFGFAIMVLSYSLFVEGTAIYDMVA
                     MAYQFPVIGAFWPLVCGLYWKRTTRQGVWWSMLLGAGVWTVLTTTAFGDVFPNVLGGF
                     LAAGFGMVAGSLAPTRGNREYLAWWHEKNRDNVVNEPLMPKPAVVPISSPA"
     gene            complement(30364..31317)
                     /locus_tag="ACZPB6_00145"
     CDS             complement(30364..31317)
                     /locus_tag="ACZPB6_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HIR1182922.1"
                     /translation="MKKFLVAFLICFSMVTTSMDADAAKRFGGGSSFGRPAPTFSQKA
                     PAPSAAPKAPQQQNAAGQQRQQPNAAPAQQAQRPSMMRSVLTGLAAALGISALLSLLG
                     IEGAGLVSLITGILAAVAIFFVLRMVLGMMMRKRVQTSSGYAEPIQYSDVPKDESQQA
                     ARTYEAPAAASGARTGSVMDQFAGGAAQDDGVRDITPDDFDRKAFLETALEQYRKLQK
                     AWDTGNVIEISDFTTTEVFTAITHQLRERGAQAYKSDIKELKNELLGITESDGVYLAN
                     IRFTGRIEIDGEVEEFDETWILEKPVEGDEGWLLGGIQQNA"
     gene            complement(31347..32123)
                     /gene="ubiE"
                     /locus_tag="ACZPB6_00150"
     CDS             complement(31347..32123)
                     /gene="ubiE"
                     /locus_tag="ACZPB6_00150"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HIR1182923.1"
                     /translation="MQNNNKDEASRQEAHHQIDFGYQMVDEDKKADQVREVFDSVAPK
                     YDLLNDVLSLGLHRVWKSRCINATETKQGQKVLDIASGTCDLAIALARRAGAGNVVAT
                     DINHEMLAIGAQRLLQAGFPCPVVEADAEMLPFADNTFDVVTVSFGIRNMTHKDRALR
                     EMLRVLRPGGRLLVLEFSKCQPWFKPIYDFYSFRFMPWAGGVIAGDRESYRYLAESIR
                     MHPDQPTFAGMMTDAGFERVSWKNMTFGICAMHIGFKPGA"
     gene            complement(32139..32561)
                     /locus_tag="ACZPB6_00155"
     CDS             complement(32139..32561)
                     /locus_tag="ACZPB6_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase family protein"
                     /protein_id="HIR1182924.1"
                     /translation="MKIPVDIALHKKSRELELVYEDGPVRLSFEYLRVLSPSAEVQGH
                     SPDEAVLQVGKRLVDITGVQVVGNYAIQFTFSDGHDSGIYSWDYLEKLVNERDVLWQQ
                     YLDDLAKNGASREPNDPANKPFEEKAAKLKPAHHCSHS"
     gene            complement(32802..32876)
                     /locus_tag="ACZPB6_00160"
     tRNA            complement(32802..32876)
                     /locus_tag="ACZPB6_00160"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32841..32843),aa:Arg,seq:ccg)
     gene            complement(32934..34949)
                     /gene="priA"
                     /locus_tag="ACZPB6_00165"
     CDS             complement(32934..34949)
                     /gene="priA"
                     /locus_tag="ACZPB6_00165"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR1182925.1"
                     /translation="MTKEAGDLYAKVMVDTPGLGLLDYAVPADMLVAVGDRVVVGLRT
                     RNVVGIVAGLRAAADFAENRVRQIKAVLRDSAPMTEEWLALTRFAAGYYIRSWGEAAV
                     PTLPVFFRRIPGVRHQNQLEKLKKLPEPAGEAGPKPALNDEQSAAVEAVSTASGYSPF
                     LLFGVTGSGKTEVYLHIMERVLARDPEAQVLLLVPEINLTPQLEGRVRSRFPGESVVT
                     LNSDLADMERARSWLAVHEGRARVLVGTRMSVFSSFRKLSLVIVDEEHDQSFKAGDGL
                     RYSARDLAVWRAHRNGIPVVLGSATPSIESWVKAQSGGYRLLELHQRAVLNALLPAIE
                     LIEPPRRGSGEALSDEACAAMSRCLEAGRQVLVFLNRRGYSPVLSCPACGWVSTCRRC
                     STFSVFHKSSRSLVCHHCGWQTEVPEACPSCGNVDILPRGIGTERLEEEIGRRFPERK
                     VLRIDRDSVSRKHEAEKAFAKVHRGEVDILVGTQMIAKGHDFYNVGLVVILNSDAQIL
                     SPDARAKERLFATLMQVAGRAGRHGDQGRVVIQTRLPEDPLFGALEKQDYRGFAESLV
                     EERKENWCVPFVHQALVTAQAKTLAEALYFLNACAKSGFSMAPEDVRVFDPVPMPLVR
                     LMDVERAQLLVEADGRASLNRFLWQWQATFNAPSGIDWTIEVDPASI"
     gene            complement(35185..36249)
                     /gene="hemE"
                     /locus_tag="ACZPB6_00170"
     CDS             complement(35185..36249)
                     /gene="hemE"
                     /locus_tag="ACZPB6_00170"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR1182926.1"
                     /translation="MKPINDCFLRALKREKTDFTPVWLMRQAGRYLPEYCETRARVGS
                     FMGLAMNPDYATEVTLQPIDRYGLDAVILFSDILTVPDAMGLGLHFVAGEGPVFDHPV
                     QSEEAVAKLYVPESDKELRYVMDAVTSIRKALNNRVPLIGFSGSPFTLACYMIEGRGS
                     RDFRTVKTMMYQRPDLLERVLDVNAKAVATYLNAQIAAGAQAVQIFDTWGGMLADGDF
                     QRFSLRYTRQVLDSLVRENEGEVVPSIVFTKGGGEWLSEIAASGCNAVGLDWTVNMTR
                     ARRVVEDKVALQGNMDPLALFGPEETIRAEARKVMDGFGMIGNGGHVFNLGHGINQFT
                     PIENVQILVDEVHEYSRRYH"
     gene            36291..36629
                     /locus_tag="ACZPB6_00175"
     CDS             36291..36629
                     /locus_tag="ACZPB6_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR1182927.1"
                     /translation="MPANPESPFTNTSWLTTGKNNAPVIAVHAQPGAKRTAVVGVHGD
                     RLKIALASPPVDGKANATLIKYLSKGLGVSKSSVRLLSGDTSREKRIEVVGLSTDDLL
                     EALKKLLSDG"
     gene            complement(36626..37030)
                     /locus_tag="ACZPB6_00180"
     CDS             complement(36626..37030)
                     /locus_tag="ACZPB6_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR1182928.1"
                     /translation="MAGVKVLVTTDGSNLSDKAIDTAIDLTKQLGGELIGMTSVVGEA
                     PTGGFEAEDAAVRDRLAAISRKAAEKGVPCEVVAEHADAIWKGVLACAVRHDVSFIVM
                     ASRGLGSIGSFLLGSETQKVLHQVDRPVLIVR"
     gene            complement(37207..37626)
                     /locus_tag="ACZPB6_00185"
     CDS             complement(37207..37626)
                     /locus_tag="ACZPB6_00185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIR1182929.1"
                     /translation="MATIKVDVVSAEESIFSGDAELVSLPGKSGELGILPGHVPLITL
                     IRPGFVRIHLPGKKEVEQVFVAGGVLEVQPDLVTVLADTAVRSNDLDEAKAAKALEDA
                     RAQRQNATGEIEIARLEAEMAALAAQIVAIKKIRQQR"
     gene            complement(37689..39092)
                     /gene="atpD"
                     /locus_tag="ACZPB6_00190"
     CDS             complement(37689..39092)
                     /gene="atpD"
                     /locus_tag="ACZPB6_00190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR1182930.1"
                     /translation="MSIGQIVQVIGAVVDIEFARDAMPKVYEALVLEKDESNPLAEEG
                     LTFEVQQQLGDGVVRTIAMGSSEGLQRGMKVKSTGHEISVPVGPKTLGRIMDVLGRPI
                     DDCGDIGAEEVRPIHRPAPKFDELSPSVELLETGIKVIDLICPFAKGGKVGLFGGAGV
                     GKTVNMMELINNIAKQYGGYSVFAGVGERTREGNDFYHEMEESHVLDKVAMVFGQMNE
                     PPGNRLRVALTGLTMAEAFRDEGRDILLFIDNIYRYTLAGTEVSALLGRMPSAVGYQP
                     TLAEEMGKLQERIASTKTGSITSIQAVYVPADDLTDPSPATTFGHLDATVVLSRDIAA
                     LGIYPAVDPLDSTSRQVDPNIIGEEHYSICRRVQETLQRYKELRDIIAILGMDELAPE
                     DKLTVMRARKIQNYLSQPFHVAEVFTGAPGKYVPLKETIRGFKMIVEGECDELPEQAF
                     YMVGTIDEAFEKAKTLK"
     gene            complement(39116..39991)
                     /gene="atpG"
                     /locus_tag="ACZPB6_00195"
     CDS             complement(39116..39991)
                     /gene="atpG"
                     /locus_tag="ACZPB6_00195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR1182931.1"
                     /translation="MPSTKEIRGKIKSVQNTRKITKAMEMVAASKMRKAQDRMFAARP
                     YGDKIRVICSHLARANSEYKHPFLVKHEGTKKIGLILVTTDKGLAGPLNTNIQRLVLH
                     KIKDWSAEGYSIEATALGNKGLGFLQRLQANVVSQAVQLGDRPNLDRLIGAIRIQIQA
                     YVEGKVDSVHLAYSRFVNAMKQVPVVEQILPLTDSHLDAADKRDHSWDYLYEPDAETV
                     LDALLKRYVEALIYQAVAENMASEQSARMVAMKAASDNAKKLIEELQLVYNKTRQAGI
                     TKEITEIVGGAAAVS"
     gene            complement(40023..41564)
                     /gene="atpA"
                     /locus_tag="ACZPB6_00200"
     CDS             complement(40023..41564)
                     /gene="atpA"
                     /locus_tag="ACZPB6_00200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR1182932.1"
                     /translation="MQLNPSEISDLLKKRIEGLGVSADLRTQGTVVSVTDGICRVHGL
                     SDVMQGEMLEFPGNTYGLALNLERDSVGAVILGSYEHISEGDLVKTTGKILSVPVGPE
                     LIGRVVNALGQPIDGKGPINTELFDVVEKVAPGVIDRQSVSQPVQTGLKSVDSMIPIG
                     RGQRELIIGDRQCGKTAVAIDTIINQKGKNLTCVYVAIGQKASTIANVVRKLEESGAM
                     EYTIVVAATASESAAMQYIAPYAGATMGEYFRDRGEDSLIVYDDLTKQAWAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNPVYVERFTEGKVTGKTGSMTALPIIETQA
                     GDVTAFVPTNVISITDGQIFLETDLFNAGIRPAINPGISVSRVGGAAQTKIIKKLGGG
                     VRLALAQYRELAAFAQFASDLDDVTRKQLERGRVVTELMKQPQYQPLQVWEEALVLYA
                     VNMGAYDDVAVADALRVERGMREYLKTHHADLIETIEAKKELSKDVDAQLKAAIESFK
                     KSGAY"
     gene            complement(41584..42135)
                     /locus_tag="ACZPB6_00205"
     CDS             complement(41584..42135)
                     /locus_tag="ACZPB6_00205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR1182933.1"
                     /translation="MAELSTIARPYAQGLMKALKDRNAGAEDVAQDLEVIEALASLTK
                     NPEVATLVGDPKLTDDQLCDLIAELCGNNLPKEVANMLRVVIENGRLEAFPEIARQFR
                     ELKNSSEGIADAYIESAFELSAAELDSLLESLAKKFPGLKLHPIVSINPELIGGVCIR
                     VGDKLLDGSIRARLAQMKTTLTA"
     gene            complement(42138..42608)
                     /locus_tag="ACZPB6_00210"
     CDS             complement(42138..42608)
                     /locus_tag="ACZPB6_00210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR1182934.1"
                     /translation="MNINASLFLQMVVFFLGAWITMKYIWPPLIHAIEERQKKIADGL
                     SAANKGEKALAIATEQGKAIEADARARATTIVSDGEKRAQSIVDEAKAQAQVEADRII
                     ENARAEAAQEMQRAREALRNEVAALAVAGAEQILAREVDKTVHAAMLEQLKAKL"
     gene            complement(42714..42956)
                     /gene="atpE"
                     /locus_tag="ACZPB6_00215"
     CDS             complement(42714..42956)
                     /gene="atpE"
                     /locus_tag="ACZPB6_00215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIR1182935.1"
                     /translation="MTNVALVALACGLIIGLGAIGACLGIAIMGAKYLESAARQPELM
                     EPLQTKMFLLAGLIDAAFLIGVGIAMMFAFANPFVG"
     gene            complement(43005..43835)
                     /gene="atpB"
                     /locus_tag="ACZPB6_00220"
     CDS             complement(43005..43835)
                     /gene="atpB"
                     /locus_tag="ACZPB6_00220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR1182936.1"
                     /translation="MAAEALTATEYIKHHMAHLASAHQGSVVDFSIVNYDTIFFSVLM
                     LAVMFFLLRAGAKKATSGVPGKMQCAVEMLVEMVNNQAKNIVHGDRTFVAPLALTVFC
                     WVALMNSIDLIPVDLFPAIAGLIGLDHLRPLPTADLNGPLGMSLGVLLLLFYYGVKVK
                     GLGGFVHELFTAPFGSHPALWPFNFLLNIIEYLAKFVSLGMRLFGNMYAGELLFFLLA
                     LLGGFALELSSIGTVSAGIGQVFAGLVWWLFHVLIVLLQAFIFMMLTLVYIGQAHSSH
                     "
     gene            complement(43848..44210)
                     /locus_tag="ACZPB6_00225"
     CDS             complement(43848..44210)
                     /locus_tag="ACZPB6_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR1182937.1"
                     /translation="MYAISDMMRISLAQYVIVFLVTVVSAFFVGQDAAYTALMAGLAY
                     AVPTTLLALFLVLPKFLTAYSPSAVAVLGGEFVKILVVVLLLGLMVGYYENLHWPAFI
                     FTIIAVANSYFIVLFKRH"
     gene            complement(44364..45242)
                     /locus_tag="ACZPB6_00230"
     CDS             complement(44364..45242)
                     /locus_tag="ACZPB6_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1182938.1"
                     /translation="MTAKKLGGLGRGLGAIYDDFDVSGLSEEDGDDKRLETVKLDELR
                     PGSTQPRSTMTEEGLQELADSIRQQGIISPIIVRPADEGYEIIAGERRFRAARMAGLE
                     EVPVIIRTVDQTQALAMALIENMQREDLNPLEEAAGIQRLIDECDYTHEQAAEAVGRS
                     RTATTNLLRLLTLTPEVKAMLKERKLEMGHARALLALDAAEQVLAAKEVVAKALSVRQ
                     TEDLIRRMKEKQPVRQKFVVKTRDDIRLEESLAETLGAVVKLSANSKGKGRIVIEFTN
                     LDQLEGIVHRIQQQGH"
     gene            complement(45259..46035)
                     /locus_tag="ACZPB6_00235"
     CDS             complement(45259..46035)
                     /locus_tag="ACZPB6_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1182939.1"
                     /translation="MAQIFCIANQKGGVGKTTTAVNLAGALALKGRRVLFIDLDPQGN
                     GTMGCGIEKRDLKGSIYEVLLGIASFEQAVCHSAEGGFDVLPANRELAGAEVELVGLD
                     HRDVRLKKALEPELDKYDYVLIDCPPSLSMLTLNALCAADGVIIPMQCEYYALEGLTD
                     LVGSVKRVRAEKNKNLRIVALLRVMFDTRITLQQQVSTQLEEHFGDKVFKTIIPRNVR
                     LAEAPSYGLPGVVYDRSSKGAKAYMACAEELIERMEKKHK"
     gene            complement(46126..46800)
                     /locus_tag="ACZPB6_00240"
     CDS             complement(46126..46800)
                     /locus_tag="ACZPB6_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR1182940.1"
                     /translation="MTSAFVSAFVLAFSTFLPVINPFSGAMFFCTLTTSLSDRDRAFV
                     ANRIALYCLIILLVCLTCGHLILSFFGISVGVLRMAGGCVLFAAGWTALNAPTQEETT
                     EERPALSRAKLKSMAFYPFTLPLTTGPGAIAVSVAVSTSLPRDLGHIAGTFVATVATV
                     AVIWGCYRYSDRLNKAVGVAGADALARIFAFILICLGVAIFWQGFSELWLELSAQATA
                     AAAAVK"
     gene            complement(46818..47456)
                     /gene="rsmG"
                     /locus_tag="ACZPB6_00245"
     CDS             complement(46818..47456)
                     /gene="rsmG"
                     /locus_tag="ACZPB6_00245"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR1182941.1"
                     /translation="MFDFQPRLEEGLATLGLEASVDQVDQLMRFAGLLQKWNKVYNLT
                     AIRRDDEILTHHLLDSAALVPFVKRLGPDAVNVLDVGSGGGLPSIPLAILRPDLRVTA
                     VDAVSKKTAFLTQAAIELGLRNYSTRHARIERLSGQYDLITSRAFANLQDFVDLTRHL
                     IKPDGLWLAMKGAVPEDEISCLPADASVAEVLPMEVPGLGEARHLVVMKPVN"
     gene            complement(47449..49389)
                     /gene="mnmG"
                     /locus_tag="ACZPB6_00250"
     CDS             complement(47449..49389)
                     /gene="mnmG"
                     /locus_tag="ACZPB6_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR1182942.1"
                     /translation="MLYPEKFEVIVVGCGHAGAEAALAAARMGRRTLLVTHNVETIGQ
                     LSCNPSIGGIGKGHLVKELDAMGGAMGVVTDEAGIQFRILNGSKGAAVQATRAQIDRQ
                     LYRRAMRERIEGQENLSVFQQAVDDLIVENGRVAGIVTQTGFCFSAQAVVLCAGTFLN
                     GMVHIGLEHYQAGRVGDPASVRLAERLKELGMPQGRLKTGTPARIDGRTIDFSKCEEQ
                     WGDLDPVPQFSLLKPAPEHPRQVPCWITRTNQKVHDIILANLDRSPMYTGVIHGIGPR
                     YCPSIEDKVKRFADKDSHQVFLEPEGLHVNEWYPNGISTSLPFDVQLDIVHNMPGLEH
                     AHLLRPGYAIEYDFYDPRGLKTSMETKAMPGLFFAGQINGTTGYEEAAAQGLLAGLNA
                     ALYARGEGSWTARRDQAYLGVMVDDLTTRGVTEPYRMFTSRAEYRLSLREDNADVRLT
                     EEGRRLGLVDDERWAYFCRKQENVARLMQSLKDTWVNPKLFTSEEMNRVLGTDMEREY
                     SLHALATRPGVTLKAVAGMTKTDGAPVLPLEDFSDDELFQAETEVKYSGYISRQKAEI
                     ERTLANETLKIPAELDYDSVPGLSFEVRQKLKSISPETIGQASRISGVTPAAVSLLLV
                     HLKRRYYYVKDKGTQAKEKGNV"
     gene            complement(49390..50760)
                     /gene="mnmE"
                     /locus_tag="ACZPB6_00255"
     CDS             complement(49390..50760)
                     /gene="mnmE"
                     /locus_tag="ACZPB6_00255"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041491300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR1182943.1"
                     /translation="MHHSDKDPIIAVATAAGRGGIGILRLSFGKALEAQIVGRLFGEN
                     CTLKARYAHLLPFLGTDGGVLDRAIVLLFPAPHSYTGESVLEIQAHGGPVLLSLIMRT
                     CLEKCRDLGLRMAEPGEFTRRAFMNGRMDLTQAEAVADLIDAVSESAVQAAGRSLSGE
                     FSNAVRDVDGNITELRAFIEATLDFPEEDIDVIGSEKVRTRLVQAETGIRAILENARQ
                     GQVLREGVTVAIVGSPNVGKSSLLNALAQEEVAIVTDIPGTTRDRIEHWVSLNGVPVK
                     MVDTAGIRTTDDVVESKGIERTLVEIRKADVVLHLKDASGLIRDDTEVLDKVLKEVRH
                     GVPLITVNNKVDLCESAASGGEIAISAKQGVGLSELKARLLEVVGMAATTDGVFMARE
                     RHLQCLKTAAEHVSRALLLIDCDPMPELVAEELRLASRALGEILGETTTEDILGMIFS
                     KFCIGK"
     gene            50879..51190
                     /locus_tag="ACZPB6_00260"
     CDS             50879..51190
                     /locus_tag="ACZPB6_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1182944.1"
                     /translation="MSLPRPPNRKPFFGPTLIGACTGMILCIPLAAYTAAALGDDYNT
                     RALIYCGFLLWCIVGAVVLFMKTWQSENQKISPAKCLLWCASLWLWPLLLLPGLFRKR
                     S"
     gene            complement(51266..52951)
                     /gene="yidC"
                     /locus_tag="ACZPB6_00265"
     CDS             complement(51266..52951)
                     /gene="yidC"
                     /locus_tag="ACZPB6_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR1182945.1"
                     /translation="MGKDMQRALLWAILFTSCFLLWDNYQVYKGEKSFFHTEAVEQQA
                     VADAASSVPTGKDIDATVPSAVAVKDPVVVETDRMKVTFDREGAVIVGTEMTLIPRQA
                     DWTEVGLAGLILDRKPAENLGNVDLLEITKNRTYVAQSGLVGGNFPNHKDQYKLVSQK
                     LSMGDADKLDVIFEATKSGVTVKKTYTFNRGHYGIDVSTEVVNNSGAAIQPSAYYQLT
                     RDGGKPEGESSMYSTYTGPAFYSDEEKFQKLEFGDIADNSASFEKFTNNGWLAMVQHH
                     FVSAWVPANGEERENYAREVSKNLYSVGTLVKLGTIESGKSVTDKAVLYSGPQDQDRL
                     EQIAPGLELVVDYGWLTFLAKPIYWLLSFLYGIVGNWGWAIVLLTCIVKAILYPVSAA
                     GYRSMARMKEVTPRMKQLQDRYKDDKQRLNQAMMELYRTEKINPVGGCLPIMLQIPVF
                     LALYWVLQGSVELRGAPWVLWVHDLAMPDPWLVLPAIMAVTMFLQILLNPKPTDPTQA
                     RMMYIMPIVFSVMFFVFASGLVLYWLTNNVLSILQQWWINKTIAAERAKRLQS"
     gene            complement(52961..53245)
                     /gene="yidD"
                     /locus_tag="ACZPB6_00270"
     CDS             complement(52961..53245)
                     /gene="yidD"
                     /locus_tag="ACZPB6_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013932.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR1182946.1"
                     /translation="MLVRLYQLTLSPWVGRSCRFVPTCSNYAIEALEKYGAVKGTYLT
                     IYRLLRCHPLGGRGLDPVPAKFRWRCWCRECRPEEQEDKGSTDLFNIQKK"
     gene            complement(53269..53733)
                     /locus_tag="ACZPB6_00275"
     CDS             complement(53269..53733)
                     /locus_tag="ACZPB6_00275"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0001682 - tRNA 5'-leader removal [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR1182947.1"
                     /translation="MSSYTAAPLKFPRSLRIIKSADYGVLVHTRNENTFRLTSKYFSI
                     NAMKFPEEPGRLRFGITVGKRNAHRSVDRALVKRTLREAARKQAPNLAALLGEQGVGL
                     DVSLRLRVPLKEIPGIDQGVNALKLSLRTDARSLMEALLKRLPKQLAQTRGE"
     gene            complement(53764..53904)
                     /gene="rpmH"
                     /locus_tag="ACZPB6_00280"
     CDS             complement(53764..53904)
                     /gene="rpmH"
                     /locus_tag="ACZPB6_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR1182948.1"
                     /translation="MATKRTYQPSKVKRARTHGFLVRSRTKGGRRVLARRRAKGRHTL
                     AL"
BASE COUNT        10687 a        17008 c        16431 g        10512 t
ORIGIN      
        1 ccaacacctg agatgcgtct cagggcctca ggggacgtcg ggcgctcgcg cactatctcc
       61 aacagggtcg catccttgaa gatgacgtag ggaggcttgc cgaggctctt cgcctcatca
      121 ctcctccact ggcgcagcgc ttcgtagagg cttctgtcac gcttggagaa ctcctgcagc
      181 agggcctccg tctgcgaatg ctcgcggcgc ttcctgcgcg tttccgccac acgctttctc
      241 acgctgatct tcatgttgcc ccgcagcagg tcgcgggcag cctctccaag gctcagcgca
      301 ttgcgcgccg tcggatccac ccagacgaca tggcgtgcga caagctggcg aagcagaatc
      361 cgccactcgg ccgccgacag gtcctgtccg atactccagg tcgagagctt gtcgtgggaa
      421 aagcgagcca cgcggtccgt catgttgccc atcagcacgt cgatgacgtg ctgagcgccg
      481 aagcagacgc cgctcgcctc gcgaatgcgg tagatgcagg agatgaactt tttggcgggc
      541 accgtcatgt cgaggaggtc gggcggctcg aggcagttgt cgcagtgacc gcagggctcg
      601 gacttttcgc cgaagtaggc cagaagcctc tgccttcgac agtcgggcgc ctcggccaga
      661 ccgagcatgg catcgagctt ctgcgtacag agctgcttgt gcatctcgtc cgcatcactc
      721 gcatcgatga agaagcgctg gttgacgacg tcgttgaggc cgtaggccat ccaggcgtcg
      781 gcgggctcgc cgtcgcgtcc cgcgcgcccc gtttcctgaa agtagccttc gatcgacttg
      841 ggcatgtcca cgtgcgccac gaagcgcacg ttcggcttgt cgatgcccat gccgaaggcg
      901 atggtcgcca ccatcacgat gccgtcctca cgcaggaaca ttgcctgatt gcgagccctc
      961 tcctctgcgg gaaggcccgc atgatagggc agcgcctcga taccgcagcc cgagaggtac
     1021 tgcgcaatct cctcggtcgt gcggcgggcc aggcagtaga cgatgccgct gtcacccgaa
     1081 tgctcgtact tgatgaagtt gaggagctgc tccttgatgt tgcgcttgtc gacgatgcgg
     1141 tagaagatgt tcggcctgtc aaagctcgcc acgaactccc tggcgtcgtt gagaagcttt
     1201 ccgacgatct ccgccctcgt cttctcgtcg gccgtggccg tgagcgcaat gcggggaacc
     1261 tgaggatagc gggtcctcag cagctccagc tctccgtact cgggacggaa gtcatgtccc
     1321 cagacgctca cgcagtgcgc ctcgtcaatg gcgaagagcg agacgttgac cctgtcgagc
     1381 aacgagagca tagaactcat catcaggcgc tcgggcgcca cgtagagcag atccagcctg
     1441 cccgacagaa gctcgtcctt cactctggag gcctcctcgg gcgtgagaga cgagttgaga
     1501 taggccgcct tcacgccgac ttcgtccagc gcatcgacct gatcctgcat gagggcgatc
     1561 aggggggaga ccacgacggc cacgccgtcg cgcagcagcg cgggaatctg ataacagagg
     1621 ctcttgccac cgcctgtcgg catcagcacc agagcgtccc tgcccgacac gacggtttca
     1681 accacctcct cctgaaagcc cctgaaggac tcgtagccga aaacctgatt tagcacctcg
     1741 atcggggtat tgaaacgtcg catatcgtcg gatgaagtca aaaaaattgc cgaagtcaaa
     1801 cttcggcaat tttaaatcag cagggcggcg caagtccgtc cctgcatttc ggaaggatta
     1861 ctccccgcgt tccagacgct cggcgcgctg gcaggcggcg atcgtgaaga gcgcgtcgct
     1921 cgaggagttc agggccgttt cgcaggagtc ctggagcacg ccgatgatga agccaacggc
     1981 gaccacctgc atggcggtgt tctgatcgat gccgaagaga ccgcaggcaa gcgggatgag
     2041 catcagggag ccgcccggca cgccggaggc gccgcaggcg cagatcgagg ccacgaagga
     2101 gaggaacacg gccgtgccga ggtcaacggg aatgttgagc gaataggcgg cggaaagcgt
     2161 cagcacggtg atcgtgatgg cggcgccggc catgttgatc gtggcaccca cgggaatcga
     2221 aatggagtaa gtggattcag gcaggccgag gcgacggcag atttccagat tgaccggaat
     2281 gttcgcggcg gacgagcggg tgaagaaggc ggccacgccg gattcgcgga gcgtcatcag
     2341 cgtcacgggg aacggattct tgtgggtgca gaaccagacg aggaacggat tgatgacaag
     2401 agccacgaag aacatggagc cgagaagcac cacgagaagc tggatgtagc cgatgaagac
     2461 ttcggcgccg gtcgtggcga aggtgttcgc aacgaggccg aagatgccga cgggggcgaa
     2521 gcggatcacg aggcggacca cgaactcgac gcccttggcg gcgtcattga gcacttcgcg
     2581 cgtggcgacg gtgccatggc gcagcacgac gcccatggcg atggcccagg cgagaatgcc
     2641 gatgtagttg ccggacgaga tggcgtggac gggattgtca acgacgttga gaatcacgtt
     2701 gccgagcacc tcgccgatgc cgccgggggt ggtggccaca gcgtcggtcg ccgtcaggat
     2761 gagcttcgtg gggaacatga agctcgccac gaccgcaacg atggcggcgg agaaggtgcc
     2821 tatcagatag aggacgatga tcggcttgat gtggaggttt tcgccggaca ggttctggtt
     2881 cgcaatcgaa ctcatcacga gcacgaacac cagaatcggg gcgacggcct taagagccgt
     2941 cacaaagacg gtgccgagga agccgcagct gacggctgcg gcgggagccg cataggcaag
     3001 cgcaatgcca agtaccagac cgaccaaaat ctgcttgaca agcgatgcct gcaggaacgg
     3061 catcgcaaaa ttgaagactc gcttcattgt tgtactccag gtgacattct tctttcgtac
     3121 ggcctttttc tgccttcggc ggccaagcct cggcggcatt cgtacgcatt tgtttacatt
     3181 accaaaaact tcacctccaa caccctcttc ttccaagctt cacagccacc aaaatgtcaa
     3241 tcagatttac aactgttgat tcgcatcaaa gcgactgggt gggttccgcc taggctcagc
     3301 ccggcctctc cggcctgcgg cagcccttcg gccccttccg cgccgccctg cgactcgcgg
     3361 taatattgga tcttcgtcag cttctttccg cgcccctgtc ccatgatgag aaagcctgca
     3421 attgggtttg ttcttgcctg cgtcttcctc gacgcactgg gcatcgggct cattgttcct
     3481 gttcttccga ggctcatcgg atcgctcgct gacacgcgcg acctgcagac cagctggtac
     3541 ggcgcaatca tggtgagcta cggcctgatg cagttcttct tcgcgcccat cctcggcgcc
     3601 ctttctgacc gcatcggacg gcgtcccgtg ctgcttacgg gcattctcgg cctttcgatc
     3661 atgatgatcg tgcccgccgt cagccagtcc ctgcccttca tcctgctctc gagacttgtc
     3721 ggcggcatga tgagttccaa catcgtggtg gcgcaggcct acatcgccga cgtgacgccc
     3781 tctcaccagc gcatcgcctc cttcggcaag atcggcgcaa tcttcgggat tgccttcgtg
     3841 ctcggccccg ccgtgggcgg cgtgctcggc cagaccgatc ccaaaattcc gttcttcgtc
     3901 gcaagcgcca tctgcgccct caacttcctc tacggcttct tcatccttcc cgagagcctc
     3961 agggccaggt cgacggctcc gctctccctc ggacgcctca acccgttctc cgccatctgg
     4021 agcctggcag gcaacctcag tcttcgcccg ctgctcgtcg tcatcacact cttcacgctt
     4081 gcacagagcc tgatgcagtg cacctgggcg ctctacacgg aataccgcta cgcctggacg
     4141 ccgctcatga tcggcatgtc gatctttgcg ctcggcctct cgatcagcct cacgcagggc
     4201 ttcatcctgc cgcgtctcgt ccagaagctc gcgccgggac gcatcatcgt gacgggcctc
     4261 ctcatcggcc tttcctccat gctcgtcatc gcgctttcgc cgtcgggcgc ggtgagtctc
     4321 gccatggtct gcctcttcgc cgtcatgggc atcgtggggc cgaccattca gggcctcatc
     4381 agcaggcgct gcgaagcaag cacacagggc gtcaacatgg gagcggtcag ctcgctcaac
     4441 agcttcacgg gcgccatctc gccgctcatc ggcactcctc tgctcatgat cacggcggac
     4501 caccacatgc atcccgtcct tgcaggcgcg ccctaccttg ccaccgttct actcatcgcc
     4561 tgcgcgctcg ctctgacatt gaagacgaac gtcggcggcg attgaaggtc aacggcccct
     4621 acagcccata acgaaagaag caccgttgga aaacggtgct tccttcattt tttacggccc
     4681 gtccggtctc ttgaccggac ggcatcaggt gcatcagatg gactcgggca ccatgtcgat
     4741 cgcgccgcag ggacactcgt tggcgcagat gccgcagccc ttgcagtact cgtagttgat
     4801 gcggaacttc ttgccgggac cgagcttgat gatggcattg tcaggacaga cgccgtagca
     4861 gttgtcgcac tcgaagcagt tgccgcagga catgcagcgg cgggcctcga agagcgcagt
     4921 ctgctcggtg agcccctgga ccacctcgtc gaacgtcgtc tgacggcgaa cgaggtcaag
     4981 ctggggacgc acggacttgg gcgcatccgc gtagtaccac gtgttgagac ggccgaaggt
     5041 cgcgggctgg cgttcttcgg cggcaggcat cggacgaccg gcaagccagg catcgatcga
     5101 gcgggcggct cgcttgccat ggccgatcgc cacggtcacg ttgcgctcgc ccggcaccat
     5161 gtcgccgccg gcgaagatgc cgggacagcc cgtcatcatc gaaccgtcga ccacgacgct
     5221 gccgttctcc atctgaaggt cgtcgatgcc ttcgaggaac ttcaggttcg tctcctgacc
     5281 gagggcgagc acgacgctgt cggcgctcac cgtctcgaac tcacccgtcg ggacgggacg
     5341 gccgctctcg tcaagcttca tcttctcgat gcggatctcg ccttcgtcgg catgcgcaat
     5401 ggtggagagc cacttcatgt gcacgccttc ctcgatggcc tcctcgatct cgaactgatg
     5461 ggcgggagcg cgctcgcggg tgcggcggta aacgaccagc ggctcggcgc caaggcgctt
     5521 tgcggtgcgg gccacgtcga tggcggtgtt gccgccgccg tagaccacca cgcggcggcc
     5581 cagaagcggc tgctcctcgc cttccatgga gcggagcacc gtaacggcgt caagcacgtg
     5641 cgcggcgtcg ccggcgggaa tgtaggctcg gcgggcaagg cttgcgccaa cgccgaggaa
     5701 cacggcgtca aagccgcctt ccttcatttc ggcggccaga tcggtcacct cgcgggagag
     5761 ctcgaacttc acgccaagct cggcgatgcg cgcgatctcc ttgtcaagaa cctcacgggg
     5821 cagacggtac ttcggaatgc cgtagcgcat catgccgcct gcggccttgg cgccgtcgcg
     5881 cacgcagacc tcatgaccca tgcgggcgag atggtaggcg gcggaaaggc ctgcaggacc
     5941 tgcgcccacg acaagaacgc gcttgccgct cttcgtctcg ggcttcttga acgaccaacc
     6001 gttttcaagc gcatggtcgc cgagaaagcg ttcgacggcg ttgatgccga cggactcgtc
     6061 gagctcgccg cgattgcagg cggactcgca cgtgtggtag cacacgcggc ccatgcaggc
     6121 ggggaaggga ttgtcctcga cgatcttctc ccaggcgtga cggtagtcgc cgctttcagc
     6181 atggtagagc catgcctggc actgctcgcc ggcgggacac ttggcattgc acggagggag
     6241 gcggttcaca tagaccgggc gcagcgtgcg ccagcttccg gtgcggttgg ccagcgacgt
     6301 gccgacatcg agcgtaatgg caaagggttt gctgctcaca gatatctcct aatcttctct
     6361 atcaaaagtg atgagcacgc tcatcaccct gaaccgccgt cggatgcgtt tcgacctctt
     6421 cctcgtcgat caggccgtag tccttgatat tgcggtccgc catggcctga atgcgggcga
     6481 tgacggtgag gtccgcgttc ttgccgaaca ggtgggcgaa gcgcttctgc ggcttcagat
     6541 actgctcgac gggaatcttg cggcggatct tgctcacgcc ggtgacttca ccgtattcag
     6601 cctcgaaaac ggggaagatg ccggtctgct gagccatgcg ggccatctga accgtcaggc
     6661 cggaaccgtg tccccagccg agcgggcaag gcacgagaat gtggatgtag cgggcaccgc
     6721 gcacactcat ggccttcgtc accttgcgct caagatcgcg aagatcggcc acggtcgccg
     6781 tcgcgacgta gggaatgccg tgcgccattg cgatgagcgg cacgtccttg ccctggcccc
     6841 aggcgttgcc gggttcgtcg cccgcgacct gggtcgtcgc cgtgcgggcg gcgggaggcg
     6901 tggcggacga gcgctgcacg cccgtgttca tgtaggcctc gttgtcgtag cagatgtaga
     6961 gcacgtcatc gttgcgttcg aacatgccgg agaggcagcc gaacccgatg tcggtcgtgc
     7021 cgccgtcgcc gcccatggcg atcacgcggg tttcagcgcc gcccgacttc ttcgccttca
     7081 ccttgaaggc ggcggccatg ccggtcgcca ccgccgcagt gttgccgaag agggagtgga
     7141 accagggcag ctgccagctc gtttcaggat agggcgtcga gaagacttca aggcagcccg
     7201 tggcgttggc cgcggccaaa cggccgttca cggcgcgcat cgccgcatca accgcgtagc
     7261 gggcgccgag cgcctcgccg cagccctgac aggcgcggtg accggacgtg agcgcattgg
     7321 tgcgctccat ggaggcctgc cccgcacgct cttcaggcgt gacaagacgg ttgcctacgg
     7381 tgaaggttcc cgtttgatag aaatgtacga cctcgctcat tctttaaatc tcctcgccct
     7441 gaagaatctt tctttcattc acttcacggt tcaacgattc ggccacgggg ccggtgtggc
     7501 gctgttcggc ctcgcgggcc agctgatgct cgacaagcgt cctgtcaagg tcgagaaaca
     7561 ccgtttcgtc ggggagcgta ccgttgcagg cggactcgaa caccgcgcgc aggctcgcct
     7621 tcgtgaccgc gcggccgcca aggccggcaa tgacgctgcg aagctccacg ggctccctgc
     7681 ggacggcctt gagcacgtcg agatagagcg cgccgccgct gcctgcgctc accatgcgtt
     7741 cgagcacgac gagcgtcttg acggacttga cggcctcgcg aagggcttcg tcggggaacg
     7801 gacgatagct cttgaggcag agcacgccga tcctgacgcc cgcctcgcgc atttcgtcca
     7861 ccgtgtcctt gatcgtgccg aggatggagc ccatggcaag cacgcagacc tccgcatcct
     7921 ccatgcgata ggtgtcgagc agaccgcccg acttgcggcc gaagcgctct gcgaagtccg
     7981 cagacgcctt ttcaatggca ggcagcgcat cgagcatgcg cttgtgggca agccagcgga
     8041 cttccgtgaa ggcctcgggg ccgaccatgg cgccgatcga gtacggatga tcgggatcga
     8101 gcacctgacg cggctcatag ggcggcagga acgcgtcgac gtcctcctgg gagggaatgt
     8161 ccatgcgctc gaaggcgtgg gtgagcacga agccgtccat gcagaccatg acgggcagcg
     8221 agagctcctc cgcgatgcgg aaggcctgga tgtgcaggtc gacggcctcc tgattggtct
     8281 ccgcaaagag ctggatccag ccgctgtcgc gctggctcat ggagtccgaa tggtcgttcc
     8341 agatgttgat cggtgcgccg atcgcacggt tggcgaccgt catgacgatc gggagtccga
     8401 gaccggacgc gttgtagacc gcctcgacca tgaagagcag gccctgggag gcggtggccg
     8461 tataggcgcg ggcgccggca accgatgcgc cgatggcaac ggacatggcc gcgaactcgc
     8521 tctcgacgtt gatgtactcg caatccttga ggacgccctt gcggcagagc gcgccaaggg
     8581 cctcgacgat gtgggtctgc ggcgagatcg ggtacgcgca gacgacttcg gggcggcaga
     8641 gcgccacggc ctgggcaacg ccctggctgc cttccaactg cttaagcatg aacacctccg
     8701 agcatcgact tgaccgaatc aaaggccagc ttcgcgactt cggcgttggc ctgggcgaca
     8761 cggcccgaga acttttcgtt gtaggcggcg atgacgcttt cgagcttcat gctgcccgtc
     8821 atggcggcca tgcccgcgag cattgcggcg ccgggcaggg ggcggccgat cttttcaaga
     8881 gcataggccg tggccggcac gcagaggaag tgcccctcag gcagacgctt cttcagatcc
     8941 tcaagtccga gagcctcggg cgtctcgctc gaattgatga gtacatagcc gtcctcgcgc
     9001 aggcccttga acacgtcgac gctctcgagc agcgtcttgt cctgcacgag cacgacgtcc
     9061 ggctcgagca cgggctccct caggcggatc tcggaagatg cgagacgggc gaaggccaca
     9121 accggcgcgc ccgtgcgttc ggagccgaag ctcgggaagg cctgtgcatg atggccctcc
     9181 atgaagccgg cgagcgccag catctccgca gccgtaacaa caccttgtcc gccgcggccg
     9241 tgaatgcgaa tttggaacac tgcagtcccc ccgtaaaaat gatggccgca ccgctatctc
     9301 ctgggcggag ctgcgaccgt tctgatcctg tgtccgaagc ctcttttgag tcatcggcaa
     9361 aacccgttaa ctctaccaag gttgacactc atgtatccga ttattatgtt tgattgtatt
     9421 agcccgcggc cgtggacgcc tgtcctattc acgctaatcc tcggccgacc cgcccgcaat
     9481 ctccgcattt cgaccgccat gcccctccgt tcgccacgtc ctcttgtcgc tttttccgca
     9541 atcgcctgtg ccgtagtgct tgcgggttgt tcgtcgctcc tgccccaggg cacgccgggc
     9601 gtctccggtc aggacggcag gagcgtctcc cactggcgcg acgccatcag ctccgccagc
     9661 aggatctcca cccgcacggc caggggccgc atgcacttcg tctgctccta cgacgccaag
     9721 ggcttctact ggcgcttctc gcatccctcg ggagggctct accgcggcaa caccaaggtg
     9781 ggcacgctca attccgactg gagcatcacc gcctccgacg gcaccaccct gaagatgtcc
     9841 gtcctcacga acggaccgag gcgaagcgcc gaggatctga ccgacgccgt cttcaaggcc
     9901 tcggccccga agaagggaac cttcgcgggc gtgcgctatg tggagcgcac caatacccga
     9961 ggcggaatgc cgctcaccaa gtgctccgca tcacagcagg gacagcgtct ttcgcgcccc
    10021 tttgaggccg actacacgtt ctggcgttga tgcctgttga aggcggaatc acattcctgc
    10081 tccgattttc ggccaagata ggccaaattt tgataaaatt gtaggtcttt actacaaaat
    10141 ttcatcggag aagctattca tggttcctgc cgctacgacg gtcggaggct atctgcaaag
    10201 ccttcccaac gaccggcgcg ttgctatggc gcgagtctgc agcatgattc gccgctctgc
    10261 ccgaggcgtt cgtgaaagca tgcgcttcgg actcgccttt tatgaactcg acgggccgct
    10321 ctttgccgtc gaatcccatg aaaagtcgct tgtcctctat ctggccgagc gagacgtcgt
    10381 caaggatttc aaagacaaga tcaagaacat cgacatcgag cactgccgcg ttgaatttcc
    10441 cgatcttaac tgcctttcgc tcgacgtcgt cgaaaagctt gtccgcgcct cccttgtctg
    10501 caggcgcgcc cgcaaggcca gcgccgaaga gccgccgtcc caagctgatc tcgtcgccct
    10561 ctggggcctg accgaaaagg acgtcgaggc cgccccgccg atcgtgcgca tcacgatcaa
    10621 ccgcgaagac gaggaagcgc ccgccgccaa ataagatgcc gcgcgtcgaa catcaaaaaa
    10681 gcctgcagct caaatgccgc aggctttttc tttttccctt cacaggctcc cgctcagccg
    10741 cggaactccc ttccgatcca ttcctcgatg atgcggctca cctcctcggg acattccgtc
    10801 agaggccagt ggaaacagtt gacgacggcc caggaaccgt cgcgaagact cctgacccat
    10861 tcctgcatgc ggcccgcatc cgtgaaggtt cccgacgacg cgccgatcac cagcacgggg
    10921 aacccgacgc cggagagatc aggcgtttcg cggcccactt cgagaatgtc gcgcatgtag
    10981 caggcggtgt gaatgtggcc gaggtccttg agcggggacg agtattcgtc gatgaaggcc
    11041 tgcagggcgt cgccgcctcg ggccaacatc tcccgcacct tgcggtcgtg cgccctgagg
    11101 ctgtagtggg gcatgcggcg gcggatgccg agcgcattga gcatgcgggc gaggaactcc
    11161 acggtgacga gaagcggata cttcctgcga tgcgcgaggg cggagggcgt aagggcggac
    11221 gtcaccagag gatccatcag caccatgccc tggattcggt ccgggtacag cacggcggca
    11281 cgcatggcga tctgtgcgcc caggctgtgc ccgacaagca cggccttctc aatgccggcg
    11341 tcgtccagca cccgcatcag gtcggccgca tgaatctcaa gcgtcgcctt ttcgcgggac
    11401 tcgctcgcac cgtgtcccct caggtcaagg cgaatgatcc gccagccttc gcgcagcggc
    11461 gtctgctccg taaactcctc ccagcggctc gcattggagc ccacgccgtg aatgagcacg
    11521 atcgtgcccg cacacgggcc gctcctgcat gaggccacgg catagctgag ggctccgtct
    11581 gcgagcgcaa cctctctgtg ctcctctcct tcgaccagat caaacagcag ttcttcgggg
    11641 catcccactt tcatttctcc tgacggctac gttcgatcca gcgatagacg gtgcgctcgc
    11701 tcacgccgag atgcgcggca aggtcggctc tggatccctt ccagcctcta attaaagcac
    11761 gcaggcgcgc ctcctcggag gcgtcccgcc cctcggcagg aacaggctcg tcgtcgaaga
    11821 gagtctcgac gccgacgatt tccccgcggc tgaagatggc cgcgcgctcg agcacggcct
    11881 gcagttcgcg cacgtgaccc cgccacgtgc ggcgggaaag cgcctcgcag acgcccggag
    11941 caagcctgag ctcgagtccc ttcgccttca ggctctgaag cgtgaggcgc acgatctcgg
    12001 gcaggtcctc cgcacgctcc tccagtccgg gcacgcggat cgggcagacg caaagcctgt
    12061 agtagagctc ctccctcagg acgtccgcct ccagcagttc ggccagcgac tttctcgttc
    12121 cgcagacgag gcggatgtcg gctcgctcgc agctcgcgcc ctccgtgtca acccagctgc
    12181 ccgtctcgag catcctgagc acggcgccct gcatgtcggc cgagagctcc tcgatgccca
    12241 tcagatacag cgtgccgccg gaaagaccga agggggcggt gagctggagc tccctgctga
    12301 gctttgcggg cgtcagcccc ctgcagtcga cgcagagaaa ggagtgtgct gcgcggcggg
    12361 agttttcatg aagcaggcgg gcgaactccc gcttgccgct ccccgaaggc ccgatgaaga
    12421 gcacgggcac gtcgagcgcg gtgacgagcg agatcttctc gaggacggcc ttcgtggctg
    12481 gcgactcggc cacgacaccc ctgagattca ggggctcctg ggtgccgttt cggtccgcaa
    12541 tcgactccat gaagagcacc gctcttccgt ccgccccctt gagcggcgtg ctgcagagct
    12601 cgagatagcg cacgcccgtc ggcccgagct ccctgcgcag caccgtctcg gacgcccccg
    12661 tgacggccgc gctctcaagc ggacagcgct cgccgcattc ggcgcagcac gacgacttgt
    12721 ggaagagaag ctcatagcag ctccttcctg caaaatcctg ccgcccgtag cgcctcacga
    12781 aggcgcgatt ggcatacgcc accgtatggt ccgccctcac gagaagcctc ggatcgtcaa
    12841 acgcgtcgag gacggtcttc aactcagaac tgactgacat atctgccact ccgcatgtca
    12901 aactgactgc catactgaca cactcatgtc gttttagtca atttgcaagt tttgttagaa
    12961 agttcctgcg gttttttcgg ctcggacggc cttagcatga acctgttgat gtgcatccgt
    13021 cgtatttccc agcaatgaaa accaacaacg tctcccctgg caagtcgaag ctcggccgcg
    13081 aaatcgcagt cgtgatcgcg tgcaagcttc tcttcatctt cggcctctgg tacttcttct
    13141 tcagtcccga gcatcgtccc gaagtgaatg ccgacgttgt cggaaacgtc gtgctgggcg
    13201 ctgcggacgc acctgccaaa gccaatccaa ccaaataaca tctggagtcc tcaatgatcc
    13261 catctgatct tgttgacctg tcccgccttc agtttgcggt gaccgcgatg taccacttca
    13321 tcttcgtgcc cctgacgctc ggactttcgt tcatgctcgt ggccatggaa gtgacgtacg
    13381 tccttaccgg caaggaaatc tacaaagaca tgacccgctt ctggggcaag ctcttcggga
    13441 ttaacttcgc cctcggcgtt gccaccggca tcaccatgga gttccagttc ggcacgaact
    13501 gggcctacta ctcccactac gtgggcgaca tcttcggcgc cccgctcgcc atcgaaggcc
    13561 tgatggcctt cttccttgag tccaccttca tcggcctctt cttcttcggc tgggaccgcc
    13621 tctccaaggg caagcatctt gccgctacga tcctcatggc cgtgggctcc aacctctccg
    13681 ccctctggat tctcgttgcc aacgcctgga tgatgaatcc cgtcggcgct gagttcaact
    13741 tccagacgat gcgaatggaa atggtcgact tctgggccgt tctcacgaac gaatgggctc
    13801 aggccaagtt cgtccacacc gtcgccggcg gctacatgac gggcgccatc ttcgtgatgg
    13861 ccatctccgc ctactacatg ctccgccgtc aggacctgca gttcgcccgc tcctccttcc
    13921 gcctcgccgc cgtcttcggc atgatcgcca ccctgatgac catccagatg ggcgacgaat
    13981 ccggctacct cgtcacgaag gaccagccga ccaagatcgc cgccatggaa gccatctggg
    14041 aaacgcatga ggctccggct ccgctgacgc tcttcgccat ccccgacgaa gccgcccaga
    14101 cgaatcacgc ccagatcgac gtgccctggc tcttcggcct gatgggcacc cgctccctct
    14161 ccaccgagat tccgggcatc aaggagctca cggcccaggc cgagcagcgc atcgtcaacg
    14221 gccagaaggc catcggcgta cttgaacagc tccgcaagga tcccgccaac gaacagctcc
    14281 gcgaacagtt cctcgccgtg cgcgacgacc tcggcttcgg tcttctcctg aagcgctaca
    14341 cgaacgacgt caacgccgcc acgcccgaaa tgatcaagca ggctgccgcc gacacgattc
    14401 cgtccgtgac cccgatgttc tggtccttcc gcatcatggt cggctccggt ctggccatgg
    14461 ccctcgtctt cgtcctcggc ctcttctatt ccttcaggaa tgaaatcgcc gagaaggctt
    14521 ggtacctgcg cctcgccgtg ctggccctgc cgcttccctg gatcgccagc gaagccggct
    14581 ggttcgtcgc cgaatacggc cgccagccct ggaccatcta caacgtcctt ccgacgcatc
    14641 tctccgtctc cgctctcacc gcgggtgacg tcgccggttc gcttctgggc ttcggcgtcc
    14701 tctacaccgc cctgctggtc gtggagatgt ggctgatgat ccgcatcagc aggcgcggcc
    14761 cgagcgctct cggcaccggc cgctaccact ttgaacgcaa ctaagccgag gctctgacat
    14821 gatcgactat gaaatcctca aagtaatctg gtggcttttc atcggcgtgc tgctcgtcgg
    14881 cttcgccgtc atggacggcc acgacatggg cgtcggctcg ctcctgccgt tcctcggcaa
    14941 ggacgacaac gagcgccgcg tgatgatcaa ctccgtcgct ccgcactggg acggcaacca
    15001 ggtctggctc atcaccggcg gcggcgccat gttcgccgct tggccgatga tgtacgctac
    15061 ggcgttctcc ggcttctact gggcgatgct cgtcctgctg atggccctca tcttccgtcc
    15121 cgtcgccttc gacttccgct ccaaggtggc cgccacgaag tggcgcacga cgtgggactg
    15181 gctcctgttc ctcggctccg ccatcccgcc ggtgatctgc ggcgtcgcct tcggcaacct
    15241 gctgcagggc gttccgttcc acatcgacga aacgatgcgt ccgatctaca cgggctcgtt
    15301 cttcggcctg ctgaatccgt tcggcctgct ctgcggcgtg ctctcgctcg tcatgatcgt
    15361 ggcccacggc ggcaactacc tgacgcttcg cactgaaggt gccctgcagg cccgtgcccg
    15421 caacgtcgcc ttcatcgcag gcatcgtctc cgccgtgctc ttcgtgatcg gcggcctctg
    15481 ggtctgggcc ggcatcgacg gctacgtcgc cgtcgccggt cttgatcccg ccggcccggc
    15541 caacccgctc gccaagaccg tcgagattca ggccggcgcc tggttcgcca acttcgcctc
    15601 cgtgcccgtg ctctggctcg tgcccgctct cggcgtgatc ggcgcgctcg gcggcgccgt
    15661 catggtccgt gcgaacaagc ccggcttcgc cttcgtgctc tcggccgcct cgctgctcgg
    15721 catcatcctg acgccgctgc tcgcgatgtt cccgttcgtc atgccgtcct cgacgatgcc
    15781 caacgccgga ctcaccctct gggactgcac gagctcgcag ctgacgcttg aaatcatgct
    15841 gttcgtcacg ctgatcttcc tcccgctggt gctcctgtac accggctggg cctacaaggt
    15901 catgagcggc aagctcaaca gcgaatacat cgagaagaac tcgcactcgc tctactgatc
    15961 gagcgcaaca tccgacaacc gggaccggcc cagccggtcc cgactgaaag aggaaacaac
    16021 catggcttac ttttactggg tgatggggct ggcctttgcc atccttctcg cttccgtcgt
    16081 tgaagtcgtc ctgtccaaga atgagcactg acgaactcaa gaagacgccc ggcgccaacg
    16141 gattcatccg cacgctgtcc atcctcgcgg ctgccgccgt cgtggctctg gtcgtgatct
    16201 atccgcgcgc cgtcgccacg gacggcgcgt cggtgccgca cggctggttc gtcctcatga
    16261 tgatgggcat gagtatctgt tggatctgcg gcttcggctt cacgccggag aaccgctggt
    16321 tgcgcaggct gctctctctg cccgtcggct ggaccatgct actgatcggc gcctggcagg
    16381 ttttttcata aggggaaagg acgtcatgtc gaatctcaat tctctctacg cacgcctggg
    16441 cggcgaggag ggtgtcattc ggctggtcaa ggcctacatc gaggctctga agacgcttcc
    16501 cggggcccag aggcttcgca gcctctaccc ggaggacctc tccaagtacg agctgcgcat
    16561 gaccgaattc ctcagcacct ggctaggcgg tcccgcgctc tatcaggagc gccacggcat
    16621 gcccatgctg agggagagcc atcgaagctt cgccattgac ggcgaggcgc gcgatgaatg
    16681 gatgctctgc atgcgaacgg cccttcgcga gacggtctcc gacgaggagc tccgcctcta
    16741 cctcgagggc gccttctggc gcatggcgga cagcctccgc accaaatgag agaagccgcc
    16801 catcaggcgg cttctctcat ttggcgcggt gcggatcaga agtccgagta ccgctcgtgc
    16861 tcggtctgca ggaccgtcac ctcgatctga agcgaatcac gatcgtcgct gatccagagg
    16921 atgccgtcct ggatcgtgac cgcaagcttc atgttgcgcg acgccatcga ggcaagcgcc
    16981 gtcacctgat cgtcgggcac gctcacgacc gtgagcttgc cgatcttggc gaagtctccc
    17041 ttgtttccct cccaccaggg ttcgacgcga tcctcgccgt aggcgacgat ctgcacgtgt
    17101 tcgctgcggc ccgcagcctt tctcacctgc ttgacgtcgg gacatcccac ctcgatccac
    17161 tggacgatcg cccccgtgtc gtcaatctcc cagagcgcgg gttcgccttc cgttgaaagt
    17221 ccgcggccga actccagacg ttcgcccgcc agccggcaga acgccagcac gcggagcatc
    17281 aggcgctcct ccgtttcgga aggatgacgt gcgatcgtga gcacgtagtt gccgtaactg
    17341 tcgcggtcca ggtccgagac gtcgatcgtc gccttgtaga cggttgctcc caaagccatt
    17401 tcgttttgtt cctttgcgtc cttcccgtgc cgccatcaaa gcggcagcgc ctcgtgacgt
    17461 ccgagcctga gccagccgta gccgatgcgg tacgcaatcc agacgccgag aacgagaaag
    17521 ccgatgatca agatgaagaa tccgatcccg aagaaaagca gggcccagcc gaagagcccc
    17581 gtcacgacga gccagagcgc cgccagccag aaggtgcgga tctgccatgc gaaatggctt
    17641 tcaaggtacg tcccctccgc gtcgccgcgg aagatgtagt tcaggatcac tgccagaatg
    17701 gaaggccacg aaaagatgaa ggcgcctgca atggaagcgc ccgtcatcag tccgagcagg
    17761 atcgagacgg tgtgcaggcc ataaacgacg tgcgcgacga tgcgcgtgcc gtcggacggt
    17821 tcgtataccc ggggttccat ggttcttcct ctaaaatgtt catgccttga cctttccgcc
    17881 aacgcatcgc catgaatttt atcagcgact gggcgctccc catccttttt cttctcgcat
    17941 ctgcggaccg aaccgccgcc atcctgatgg cggtgctgtt tgcgaccgcc cgcatcacgg
    18001 gacccgctga agcggccggc gccgtcgcgc tcggctcggc gtgcctcacg ctcctgctgt
    18061 ggcggatcgg cctgcggcac aaaaagagag aggaggttcc gccgccttct gcctgcgact
    18121 atacaatagg gcagtggctc cggaaacacg gtccggcagg cgcggccgca agagtcgtgc
    18181 ccgcagtcgg atggcgcttc agcggcaccg tcgcgcttgc ctcgcaaagc ggcggcaggc
    18241 ccgccgtctg cctctatgcc gtcctcttca ccgtgctctg ggtcctcttc tggattcttg
    18301 tcctcatgcc tgcggccgag cggctcatgg ctgcgacgac cacgctcacg cacgtaagga
    18361 tgacgctgcc cggcctgatg ctgctcgcct tcacggcggc cgtcgtgctg aggaccgcgc
    18421 tctgccgcct gcatcaacga tatcaagaga aaaaacatga gaattcttgt cgctgaagac
    18481 gatgacatga tcggtgaaag cctcgttgcc ttccttgaaa gcgaggacta cgcagtcgac
    18541 tgggtccgtg acggacagtc cgcgctgctc gccatgaaga ccacgccgtt ttccctcgtc
    18601 atcctcgatc tgggcctgcc cggcaaggac ggccttcagg ttctcaagga ggtccgcgcc
    18661 cgacgcgacc cgacgcccgt tctcgtgacg accgcccgcg ataccgtcga cgaccgcgtc
    18721 aagggtctcg acagcggcgc cgacgactat ctggtcaagc cctatgagct cgatgagctc
    18781 tcggcccgcg tgcgcgccct gctgcgccgc tccgcaggcc gtgcggaccc gatcatcgaa
    18841 cgcggcgccc tgaggatctc ccccgccacg cgcgaagtca ccttcaacgg tgagcccgtc
    18901 atcctctcct ccaaggagta tgctctgctc gcagccctcg ccgaacgcca gggcgtcgtc
    18961 tggagccgct cccagctcga ggagaagctc tacaactggg acaataccgt gggctcgaac
    19021 gccatcgagg ttcacatcca ccatctgaga aagaagctct ccgacaacgc catcaagacc
    19081 gtgcgcggcg tcggctacct tctcgaaacc tagtccatgc actccatccg caggaagctc
    19141 ctgattacgc tgatcggcgc gctgctcgcg gccggcttca cgacggccgc ggcaacgttc
    19201 ttctccgccc agacggagtt caacaagttt ctcgacacgc atttgaagga aacggcggaa
    19261 tcgcttgcgc agtccgtcac gcactccttc ccgcttgccg atcccgatca cctgaccatc
    19321 atcggcgacg cgcagtccta ccacatcgtg gtccaggtct acgactccgc gaacaacacc
    19381 gtctggcagc gcgaggatca gccatacctg ccgcttcccg acgcgccggg ctttgccaga
    19441 gccaagctca acggtgtcaa ctggcgcacc tactctacgc cggctggccc gctcatcatc
    19501 acggtgggcc aggacacggc cgtgcgaacc gaactcgccg cctcgtccgc cttccgcgtc
    19561 ctgcagcctc tctgcctgct tctgcccttc atcgcgatcg ccgtctggat cgtggtgggc
    19621 agcggcctcg caccactcga gaagacggca cgctccgtcg cacgccgatc gcccacgtct
    19681 ctcgaaccca tctcgacgaa ggggcttccg cttgagctcg caggcctcgt cagcgccatc
    19741 aaccaactgc tggaccgact gcgcgaaagc ctctccgcac agcagcgctt cgcctccgac
    19801 gccgctcacg aactccgcac gccgctgacg gccctcaagc ttcaggttca gctcgcacag
    19861 cgcgccaaga ccgacgaggc gcgcgagaag agcctcatgc gcctcaacga gggcatcaac
    19921 agagccaccc gcctcgtgca gcagctcctc acgcttgcgc gtctcgatcc cgattcgacg
    19981 aagcatcccg caagcacgat caagctcgac agcctcgccc acagcgtgtg cgaggacatg
    20041 acgcccattg cctcccagaa gagcatcacg gtcacggccg tcacgaaacc cgcctcaacg
    20101 gaagggctcg aggacgccgt gcggctgatg atgacgaacc tcacggacaa cgccgtgcgc
    20161 tacacgcctg agggcgggcg catcgaaatc cgcacccgaa ccgatgcgga caccgacggc
    20221 gcagtcatcg aaatctgcga caacggtccg ggcatcgccc ccgaggagcg cgaacgcgtc
    20281 ttcgaccgct tctaccgcgc gctcggcacc aagacctcgg gcacgggcct cgggctcgcc
    20341 atcgtcaagc gcatcgtcga catccatcat ggtacgatcg caatcgacga cggccttgac
    20401 gggcgcggca cgaccttccg catccgcctt ccgaagctcg gcgcagccgc tgcggctgtc
    20461 tgacagggac ttcccttctg ttacaaattt aacccctgtt gcacaaccat tacctgtagc
    20521 tgataccccg tccatccgtt ttgcgataaa gttgttggat caattgggaa acaatgcggc
    20581 ccagccgcat cgtttccggg actcacactc aggaaaaaag catgtttgca tcgaaaatcg
    20641 cccgctccgc gacgctctct ctggcggtcg cggccgtcat cagctccatc gccgttccgg
    20701 ccactgccgg cgtcaccatc ttcggcggct ctgaagacaa gcagcaggtg caactccccg
    20761 acttcgtgaa gctcgtcgag gacaacggcc ccggcgtcgt gaacatccag atgatccgca
    20821 acgcccgcac agttgaaaac gccggcattc cgggccttga tccgcgcggc gcagagatct
    20881 tccgccgctt cggcattccc ttcgacttcg gtccgcagga aattcccgag cagcgcggca
    20941 cgggctccgg cttcatcgtc tcctccgacg gcatcatcat gacgaacgcc cacgtggtcg
    21001 agggcgccga tgaactcatc gtccgcctca ccgacaagcg tgaattcaag ggcaaggtgc
    21061 tcggctccga caagcagacc gacatcgccg tcatcaagat cgaagcgaag gacctgcccg
    21121 tactcaagat aggcgactcc agcaagctca aggtcggtga atgggtggcc gccatcggca
    21181 gcccgttcgg cctcgacaac accgtgacgg ccggcatcgt ctccgcgctc tcgcgcaacc
    21241 tcccgagcga ccagtatgtg ccgttcatcc agaccgacgt ggccgtcaac cccggcaact
    21301 ccggcggtcc gctcttcaac atgcaggggg aagtggtcgg catcaactcc cagatcttct
    21361 cgacgtcggg cggcttcatg ggcctctcct tcgccatccc gatcgatctg gccatgcaga
    21421 tcaaggacca gctcgtcaag gacggcaggg tcacccgcgg ctatgtcggc gtcttcatcc
    21481 aggaaatcaa tcaggagctc gccgacagcc tcggcctcaa gacgcctgag ggcgccctcg
    21541 tgacgaagac cgaaaaggac agccccgccg aaaaggcagg cctgcgtgag cgcgacgtca
    21601 ttcttgcgct caacggcaag aaggtcacct cctccgtcac actgccctct ctcgtgagca
    21661 ccatccgtcc gggcaccgaa gtcacgatga cggtcttccg cgacggcaag gagcaggaaa
    21721 tcaaggtgac ggtcggctcc aacaaggcga ccgaggagaa gcaggccacg gcagccaatg
    21781 aaacccgcct cggcgtgacg acccgcccgc ttacgaagga ggaggagaag aaggctgaaa
    21841 ccaccggtct tctcgtcacc caggcttccg gtcccgccgc caaggcaggc atccgtgcgg
    21901 gcgacatcct cgtgagcgcc gacggcaagc tcctcaagag ccgagacgat ctgctcgagc
    21961 agtgcaagga caacaaggtt ctccttctcg tgcagcgcaa cggcggacgc atcttcatcc
    22021 ccgtcaagct tcaggccaag aaataagatc cgtcacaaac aggggctccc tcagggagcc
    22081 aatcggatag gggtgaggac tttcggtcct cagccccttc cacaccaccc accgtgcgga
    22141 tccgcagtgg gcgattatca ataacctcag agaacatcct acttgtaaac gtcccctagg
    22201 cacgtccatc ccaagtttcg gagtcggtca tttgtcagcg aggtcgagag tatccaactg
    22261 tttgccgtat gccagtacga tttgcgggta ttggcccact cccaagccac cctgtcgtct
    22321 gcgcccagtt tcctgagcgc cgcaaactta gtccgaacct tcttccactg tttccagtag
    22381 atctgacgga tgcgtctgcg caaccattca tcggtgcgtc tgacgaattt cgccatcgtc
    22441 gacgccgcga aatagttgac ccagccacgg acgaaagcct tcagctcctt tctgtactct
    22501 tcaaggcttc tgctgacttc tcacggcaag ctttactcca tcgccctgtt ggggcggtgt
    22561 ccgtgagatc tcctcgggta gaacacgctc tttcccctca tatagctgcc tcatttacac
    22621 cgacggttcc gtgcagttat tggacttcgt cttgccttgc agacttatcc accgtcatat
    22681 gcctgatgag gttcttgttc atcatctcaa gggcttgcct ccagcttctt tcagcctacg
    22741 cctcgcgacg cacaccttgc ttttggctat gaggttgcca ctgccggccc ctctcgggac
    22801 tttcacccgt tagaacgtgc ccatgccgag cacaccgaag caaaaaggct gccccttcgg
    22861 gcagcctttt tcattaccgg tattcaggaa ctgtcccttc aggacgcgtc ctcgttctcg
    22921 tgatacttct tcttgctgtg catcatgact ttgagcttgt cgttgtcgac ctccctgcac
    22981 cagtggccga ccacgacaca ggcgatgccg ttgccgatca tgttggtgag ggcgcgggcc
    23041 tcggacatga agcggtcgat gccgagaatg agtgcaagac cggcgacagg cacatggccg
    23101 acggcggaga gcgtggcggc aagcacaatg aagcccgaac ccgtcacgcc ggcggcgccc
    23161 ttcgaggtga ggagcagcac ggcaagcagc gtgagctgct gagtgagcgt catggagacg
    23221 tcgcaggcct gggcgatgaa gaccgcagcc atcgtcaggt agatggcggt gccgtcaagg
    23281 ttgaaggagt agcccgtcgg aatgacgagg cccacgacgg agcgggacac gccggcgagc
    23341 tccatcttcg tcatcatgcg cggcagcacg gactcggacg aggacgtgcc gagcacgatc
    23401 aggagctcct ccttgatgta ggagatgtag cgcatgatcg agaagccgtg gatgcggcag
    23461 atggtgccga gcacgccgaa gatgaagaga aggcaggtca gatagaacgt gcccatcagc
    23521 ttcgccagcg gcagcagcga agcgatgccg tacttgccga tcgtgaaggc catggcaccg
    23581 aaggcgccga cgggggccac gcgcatgatc atgccgacga tctggaagag aacgtgtgcg
    23641 gacttctcga tcacatcgaa gatcagcgtg ccgcgaccgc cgaacttgtg cagcgcgaag
    23701 ccgaagagga tggagaagaa gagcacctgc aggatgtcgc cgttggcaaa ggcgcccacg
    23761 acggtattcg ggatgacgtt catcaggaat tccgtcgtcg tctgcatctt gccgggaccc
    23821 gtataggcgg aaaccgcagc ctggtcgagc gtgttcggat cgacgtgcat gccgacgccg
    23881 ggctggaccg tgttgacgat gatgaggccg atgatgagcg cgatggtcga gacgacctca
    23941 aagtagagaa gcgcaagacc gccggtcttg ccgaccttct tcatgtcctc catgcccgca
    24001 atgcctgtca cgaccgtgca gaagatcacg ggcgcgatga tcattttgat gaggcggatg
    24061 aagccgtcgc cgaagggctt catctgagca ccgatctcgg gtgcaagaaa gcccaggaga
    24121 atgccgatga caatggcgca gatgacctga aagtacagca ccttgtagat cggagtttta
    24181 tggtttgcag aagccgcgga catttatgag gtctccgggt tggccgacgg acgaatccgt
    24241 ctgccgtgta atttttttct ggatggagcc tccgggaagg aggcagcgat cgtcatcaga
    24301 aacgcccccc gaaaaggacg ttttgaagga ctcccaataa taaggcactt cttgcatcca
    24361 tagcggattt tcttaatggt tattccttat gtaccgcgaa gtccttaggg cgttcaccta
    24421 atccgacgta caaaaacgct cgtctgcata cgacaaaccg cccatgtctt gcaaaacacc
    24481 gcgcaatgtc ttaatgtgac gcatcccttt ttcggccccc tttccgacta ggtgccggtc
    24541 ctcagctcaa tcatgttgga agccatccat tttcagtatt ttctcggcgg tctggtgtcg
    24601 ctgctcgtga tcaccaatcc gctctcgaag ctcccgctct tcgtcagcct gacgcagggc
    24661 atgtccgagg agcgccgccg ccaacaggcg aactgggccg gcatctatgc cggcatcatc
    24721 atggccgtaa gcctcgtggg cggcaacatg ctgctcgcgt tcttcggcat ctcctacggc
    24781 gccatgcgaa tctccggcgg cctcgtcgtc gcagccatcg gctatcagat gctgttcggc
    24841 ggaagctcgc cgaacaaggc gcccatcgtg cgacgcgaca aggacgacta cgccttcttc
    24901 ccgattgcca tgccgggcat cgccggcccc ggtacgatcg ccgtcgtgat cggcttctcc
    24961 accgagatcg ccgagctttc caactatgcg gacatcgcca tcgccttcgg ctggacggtc
    25021 cttgcgatca tcttcacggc catggcctcc tgggccgtca tgcgctactc cgacatcctg
    25081 acccgcaggc tcggcccctc cggcacgctc gtgctgggca agctcatggg cttcctgctc
    25141 atctgcatcg gcgtgcagtt catcggctcg ggcatccgca cgttcatggc gggcggctag
    25201 tccgtttcac acctccgaat tgaaaaggcc gcagcccctg atgggttgcg gcctttgctt
    25261 cttgcttcgt ctgcagggcg cggtcttatt cgaccgtcac gctcttggcg aggttgcggg
    25321 gcttgtccac atccgttccg cgggcaagag ccgtgtggta cgcaaggagc tgcagcggca
    25381 ccacgtgaag gatcggcgaa agacggccgt agttttcagg catgcggatg acatgcatgc
    25441 cctcggacga ctcgatgacg gagtcgccgt cggcgcagac atagagttcg ccgccacggg
    25501 cacgaacctc ctgcatgttg ctcttgagct tttcaagcag gcggtcgttg ggcgcaaccg
    25561 tcacgaccgg catttcgcgg tcaaccagtg caagcggacc gtgcttgagc tcgccggcgg
    25621 gataggcttc ggcgtggatg tagctgatct ccttgagctt cagggcccct tcgagcgcga
    25681 tcggatagtg cacgccgcga ccgaggaaga gcgcatgctg cttggcggca aagctttccg
    25741 cccaggcgat aatctgaggc tcgagcgcga gcacggcgct cattgcggct ggcagatggc
    25801 gcaggcgctt gagctccgtc tcctcatcct ccttcgtgag gcggccgttg agcttggcaa
    25861 gcgtgcaggc aagcagatag agagcggtaa gttgcgtcgt gaaggccttc gtggatgcaa
    25921 cgccgatctc gacgccagcg cgcgtgatgt aggccaaacg gctctcattc accatggccg
    25981 acgtcgccac gttgcagatg gtgagcgtct gggacatgcc cagctcgtcg cgggcatggc
    26041 gaagggccgc caacgtatcg gccgtttcac ccgactggga gatcgtcacg acaagcgtcc
    26101 tcggatcggg cacggagacg cggtagcggt attcgctcgc gatctcgacg tcgcacggaa
    26161 tgcctgcgat ctcctcgatc cagtacttgg ccgtgagagc ggaatagtac gacgtgccgc
    26221 aggcaaggaa gagcaggcgc tgcgtgttgc ggaagatgcc ttcggcttcg gcgccgaaga
    26281 gcgtggggga gaaggccgcc acgccttcga gcgtgtcgcc gaccgctcgg ggctgctcga
    26341 agatctcctt ctgcatgaaa tgacggtagg gcccgagctc gacggcgccc gcataggcgt
    26401 gaaccacctt gacctcacgg tcggcgggaa tgaattcacc cgcctccgtg cgctcgtaga
    26461 cctgccagga atccggacga acgtcgacga cgtcgccctc gtgaacatag acgatgcgat
    26521 ccgtcgtgcc tccgaccgcc atggcgtcgg aggcaatgaa gttctcaccg tcgccgacac
    26581 ccacgaccat gggcgagccc tgacgggcgg cgatgacgcg gtcgggctcg tcggcagcga
    26641 tcacggcaat ggcatagctg ccgcgcaggc gcgtgacggc tgtcatgacg gccttgagca
    26701 gatcgccgtc atcgttgtcg ttgatgagat gtgcgaccac ttccgtgtcg gtctggctcg
    26761 tgaactcata gcccttggcc tgaagctcgc ggcggatctc ctcgtgattc tcgatgatgc
    26821 cgttgtgcac cagcgcaatg cggttgcgcg agatatgcgg atgagcgttg cagacctcgg
    26881 gagcgccgtg ggtggcccag cgcgtgtgcg caatgccgac ggtgcccttg acgccgtctt
    26941 cggccacgcg ctcgacaagc tcgtgcacgc gggacacggt gcgggccctc ttcaggacgc
    27001 cctgatcaag cgtggcgacc ccgcaggagt catagccacg gtattcaagg cgacgaaggc
    27061 cttccaaaag catgggcacg acatcgcgat gactcgcggc ggcgacgata ccacacattt
    27121 ctttctctcc aaacgttctt cgggcggccc tgcggccgcc cggtttcttt ctgtcttact
    27181 gcttcttgac cggacgcgtc cagccctcga tcgtcatctg acgggcgcgg gaaagcgtga
    27241 gcttgcccgc gggcgcgttc ctcgtaagcg tcgtgcccgc accaatcgtg gcgcctgcac
    27301 cgacctcgac gggcgcgatg agctgggtgt cggacccgat gaacgcatcg tcgccaatga
    27361 ccgtgcggaa cttgttcacg ccgtcatagt tgcaggtgat cgtgcctgcg ccgacattga
    27421 cgcgcgcgcc cacgtcggcg tcgccgatgt aggtgagatg gttgaccttc gaatcctgac
    27481 cgatgacgga cttcttcacc tcgacgaagt tgccgatgtg gttgttgccc gtgagttcgg
    27541 cgcccggacg aagcctcgag tacgggccga tgcaagagtc cctgccgacc gcggcgcctt
    27601 caaagtgaca gaagggaagg atttcgacgt catcctcgac cgtcgtattg cgaatcacgc
    27661 tgtaggcgcc cacgcgaacg ccgttgccga gcacgacgcg gccctcgaac acgcagccga
    27721 cgtcgatctc gacgtcctgt ccgcagacaa gctcgccgcg aatgtcgatg cggtcgggat
    27781 caatgagcgt cacgccggct tcaagcaggc ggtcggcctg cacgcgctgc caggtacgtt
    27841 caagggcggc gagctgcacc ttggagttga cgccctcgac ttcataggca tggtcgggat
    27901 gaacggcgga gatgcgcacg ccgtcggcgg ccgcgaagcc cacgagatcc gtgaggtagt
    27961 actccttctg ggcgttgtcg ttcttcaggg cgtcgagcca ggcgtcaaga cgggccgtcg
    28021 ggagcaccat gatgccggtg ttgacctccc tgatcaggcg ctgctcgtcg gtcgcatcct
    28081 tttcctcgac gatggccgtt acgacgccct cggcgtttcg cacgatgcgg ccgtagccct
    28141 tcggattgtc catttcaatg gtgaggagaa cgaactcgct cttctccgcc tcctcgagca
    28201 tgcggcgggc cgtttcagcg gacagaagcg gcacgtctcc gaggcagaca agcgtctggg
    28261 gttcgtttcg gtcgagcacg ggccaggcct gcatcagtgc atgccccgtt ccttgctgcg
    28321 gcatctgaag cgcgaactgc aggtcggtcc tgccttcgta gtgcttctgc acctcctccg
    28381 cgcaatgacc gacgacaacc gtcttgacgg cggcaaagcc ctccacggca tcaagcacgt
    28441 gatcgagcat cggtcggcct gcgagaggct ggagaacctt gggcagtttg gaatgcatgc
    28501 gcttgcccat gccggcggca agaatgacga tgttcatgga tatgcgtggt cctggaatgc
    28561 gtgactctga aacaagaagc gggagccctc atcgagggct cccgcctgtg actggtagat
    28621 gaattttatc aggccggcga gctgatcgga acaacggccg gcttgggcat cagaggctcg
    28681 ttgacgacat tgtcacgatt cttttcgtgc caccaggcca gatactcgcg gttgccacgc
    28741 gtcggggcga gcgagcccgc caccatgccg aagcctgcag ccaggaagcc gccgaggacg
    28801 ttcgggaaca cgtcgccgaa cgccgtcgtc gtcagcaccg tccagacgcc ggcgccgaga
    28861 agcatcgacc accagacgcc ctgacgggtc gtgcgcttcc agtagagccc gcagacgagc
    28921 ggccagaagg cgccaatcac ggggaactgg taggccatgg ccaccatgtc gtagatggcg
    28981 gtgccttcga caaagagcga gtacgacagc accatgatcg caaagccgaa caccgtccag
    29041 cgcatcgcac ggagcatgtt ccggtcgttg aggcggacga agtgcgtgag gatgttgctc
    29101 acaaaggcag tcgacggagc gagcatcgtg gcggacgcag tcgacatcac ggccgagagc
    29161 acggcgccaa agaaaagcac gcggagccag agcggcatgt ggtcacgaac gagcgtcggc
    29221 aacaggcgct gcggatcggt ggcgagcatg tcctgcgcgc tgctgggcat taccagaatg
    29281 gcggcgatga cgatgaacat cggcacaaac gcaaagagga tgtagacgca gccgccgatg
    29341 acggggccgg ccacggccgt ctgcacgttt ctggcggaca tgacgcgctg gaagacgtcc
    29401 tgctgcggaa tcgagccgat catcatcgtg atggcggagg atatccaaaa gagccacccg
    29461 gtcgccgtcg gctcaggaag cgcattgaag agatcgcgct catgagcgta gtcgatcacc
    29521 ttgccgaaac cgcccgcgag atccgacgca aaaaagagga tcgcaacgag gccgagcacg
    29581 atgatgatca tctgcacgaa gtcggtgact gcgacggacc acatgccgcc gtacatcgtg
    29641 taggcaagca caacgacggt gccgatcacc atgccgccgg acacggagat gtagccctcg
    29701 gtgatcaggt tgatcacgag accgagcgca gcgacctgag cccccaccca cccgaggtag
    29761 gagaggacga tgaaaagcgt gcagagaacc tcgacgccgc ggccgtagcg ctcgcggtag
    29821 tagtcgccga tcgtcaggat gttgagcttg tagagcttct gtgcgaagaa catgcccaca
    29881 agaatcagag ccatgccgga gccgaacggc tcttcgatca cacctgcaat gccgctctcg
    29941 atgaagcggc cgggaagacc cagaatggtt tcggaaccaa accaggtggc aaacgttgcc
    30001 gtgatgacca taggcagcgg aagactgcgc cctgcaaggg caaaatcact cgtactgctc
    30061 acacgcgttg cggcatagag gccgatggcg atcgtcgcca gcatgtagag cattacaaaa
    30121 ccaatcagcg ccatctcaca tatttcctca taaaacgacg ttgaagggaa aaccttgttt
    30181 attcgcgcga attgtagggc gtattgcctc ccaccgtacc ctgcagaatg taaaaaatcc
    30241 gacctcgggg acaccaatcg accattgttt catcgagatc aaaaaaaacg tgtcctttcc
    30301 ctcagccaga gggaaaggac acgtttttta tatgacaaaa cgatgacaga aatcgacggc
    30361 ccgtcaagcg ttctgctgaa tgcctccgag gagccagccc tcgtcgcctt cgacgggctt
    30421 ttcgaggatc caggtctcat cgaactcctc gacttcacca tcgatttcga tcctgcccgt
    30481 aaagcggatg ttggcgaggt agacgccgtc ggactcggtg atgccgagca gctcgttctt
    30541 gagctccttg atgtcggact tgtaggcctg agcgccgcgt tcgcgcagct ggtgcgtgat
    30601 ggcagtgaag acctcggtgg tcgtgaagtc ggagatctcg atcacgttgc ccgtatccca
    30661 ggccttctgc agcttgcggt actgttcgag cgccgtctcg aggaaggcct tgcggtcaaa
    30721 gtcgtcgggc gtgatgtcgc gcacgccgtc atcctgagcg gcaccgcctg cgaactggtc
    30781 catcacgctg ccggtgcggg cgccggaagc agccgcgggc gcttcgtacg tacgggcggc
    30841 ctgctgcgac tcatccttgg gcacgtcgct gtactgaatc ggctcggcat agccggaaga
    30901 ggtctggaca cgcttgcgca tcatcatgcc gagcaccatg cgcagcacga agaagatcgc
    30961 cacggcagcg agaatgcctg tgatgaggct caccaggcct gcgccctcaa tgccgagcag
    31021 cgatagaagc gcagaaatgc cgagtgcggc ggcaaggccc gtcaggacgc tacgcatcat
    31081 ggacggacgc tgggcctgct gcgcgggagc ggcgttgggc tgctggcgct gctggccggc
    31141 agcattctgc tgttggggcg ccttgggcgc ggcggacggc gccggagcct tctggctgaa
    31201 agtcggtgcg ggacggccaa aggagcttcc gccgccgaag cgcttggcgg cgtcagcatc
    31261 catgctggtc gtcaccatgc tgaagcagat caaaaaagca acgagaaatt tcttcatggt
    31321 cgttggtcac ttggtggtgg aaaaaatcag gcgccgggct tgaagccgat gtgcatggcg
    31381 cagatgccga aggtcatgtt cttccaggac acgcgttcaa agcccgcatc cgtcatcatg
    31441 cctgcaaaag tcggctggtc ggggtgcatg cgaatggact ccgcaagata gcggtaggac
    31501 tcgcggtcgc cggcgatgac gccgcccgcc cagggcatga agcggaagga atagaagtcg
    31561 tagatcggct tgaaccaggg ctggcacttg gagaactcca gcacgaggag cctgccgccc
    31621 ggacgcagca cgcgcagcat ctcgcgcagg gcgcggtcct tgtgcgtcat gttgcggatg
    31681 ccgaaggaca cggtcacgac atcaaacgtg ttgtcggcaa aggggagcat ttcagcatcg
    31741 gcctcgacga cagggcacgg gaagcccgcc tgaagaaggc gctgggcgcc gatcgcaagc
    31801 atctcgtgat tgatgtcggt ggccacgacg ttgccagcgc cggcacggcg ggcaagcgcg
    31861 atcgcaaggt cgcaggtgcc gctggcaatg tcgagcacct tctggccctg cttcgtttcg
    31921 gtcgcattga tgcagcggct cttccagacg cgatgaagcc cgaggctgag cacgtcgttg
    31981 agaaggtcgt acttgggcgc aacgctgtcg aagacctctc gaacctgatc ggccttcttg
    32041 tcctcatcga ccatctggta gccgaaatcg atctgatggt gagcctcctg tctggaggcc
    32101 tcgtccttgt tgttgttctg cattttttgc ggttgttgtc aggaatggga gcagtggtgt
    32161 gcgggcttca gcttggcggc cttttcctca aagggcttgt tggccggatc gttcggttcg
    32221 cgggaggcgc cgttcttggc aagatcgtcg agatactgct gccagagcac gtccctctcg
    32281 ttgacgagct tttcaagata gtcccagctg tagatgccgg agtcgtggcc gtcggagaac
    32341 gtgaactgga tcgcatagtt gccgaccacc tgcacgcccg taatgtcgac gaggcgcttg
    32401 ccaacctgaa gcacggcttc atcaggcgaa tggccctgca cttcggcgga cggagacagc
    32461 acgcgcaggt attcgaagct cagacggacg gggccgtctt cgtaaacgag ttcgagttcg
    32521 cggctcttct tgtgaagagc aatatcaacg ggtattttca tcttccatca tctccgcccg
    32581 tcctcttgac gggccgtatc tgtcgtttcg gcgcggtccg aagaccgttt gagcagccgt
    32641 ttttgaagtc ctgtaatgat aaccgcttca gacggccacc acagcggcgc ccgcacctca
    32701 ttacacacca tccgccctgc cgtcagcctt aacgacgccc gccttccgta atcttcgggc
    32761 aaagaaaatc ccgcctcgaa gatcgagacg ggatccgaaa atggtccccg cgacaggaat
    32821 cgaacctgta ccgcagcctt cggagggctg catgatatcc atttcaccac gcggagaaga
    32881 aggtcattat aacggaattc cctccgttca aaaaacgtcc ggaaggtgcc cgactagatg
    32941 gatgccgggt cgacctcgat ggtccagtcg atgcccgagg gcgcattgaa ggttgcctgc
    33001 cactgccaga gaaaacggtt cagggatgcc ctgccgtcgg cctcgacgag caactgggcg
    33061 cgttcgacat ccatcaggcg cacgaggggc atgggcacgg gatcgaagac cctgacgtcc
    33121 tcgggagcca tgctgaagcc tgactttgcg caggcgttga ggaagtagag tgcctcggcg
    33181 agcgtcttcg cctgcgccgt gaccagtgcc tgatggacaa agggaacgca ccagttttcc
    33241 ttgcgttcct cgacgagaga ctccgcaaag cccctgtagt cctgcttttc cagagcgccg
    33301 aaaagggggt cctcgggaag ccgcgtctga atgacgacac gcccctgatc gccgtggcgg
    33361 ccggcacggc ccgccacctg catcagcgtg gcgaagagcc gctccttggc gcgcgcatca
    33421 ggactcagga tctgggcgtc ggaattgaga atgacgacca gaccgacatt gtagaagtcg
    33481 tgccccttcg cgatcatctg ggtgccgacc agaatgtcca cctctccgcg gtgtaccttg
    33541 gcgaaggcct tttcagcctc atgcttgcgg gagacactgt ctcggtctat gcgaagcacc
    33601 ttgcgctcgg ggaacctccg gccgatctcc tcctcaagac gctccgtgcc gatgcctcgc
    33661 ggcagaatgt cgacgttgcc gcaagaggga caggcttcag gcacctccgt ctgccagccg
    33721 cagtggtggc agaccagcga tctggaagac ttgtggaaga cggagaaggt ggagcagcgc
    33781 ctgcacgtgc tcacccagcc gcaggcgggg catgacagca cgggcgagta acctcgcctg
    33841 ttgagaaaca caagcacctg acggccggct tccagacaac ggctcatggc tgcgcacgcc
    33901 tcatcggaga gcgcctcgcc cgagccacgc ctcggcggct cgatgagctc gatggcaggc
    33961 agcagggcat tcagcacggc gcgctgatga agctcaagaa gcctgtagcc gccagactgt
    34021 gccttgaccc agctttcaat cgaaggcgtg gcagagccta gcacgacggg aatcccgttc
    34081 ctgtgggctc gccagacggc aaggtctcgg gccgagtatc gaagtccgtc ccccgccttg
    34141 aacgactggt catgctcctc gtcgacgatg acgagggaaa gcttgcggaa gctggagaag
    34201 accgacatgc gcgtgccaac gagcacgcgg gcgcgcccct cgtgtacggc aagccaggag
    34261 cgcgcgcgct ccatgtccgc cagatctgag ttgagcgtca cgacgctctc gccgggaaag
    34321 cgtgatcgca cgcggccctc gagttggggc gtcagattga tttccggtac gagcaggagg
    34381 acctgagcct cgggatcacg ggcaagaacc cgctccatga tgtgaagata gacttcggtc
    34441 ttgcccgacc ccgtcacgcc gaacagaagg aaggggctgt agccggaagc cgtcgagacg
    34501 gcctccaccg ccgcactctg ttcgtcgttg agcgcgggct tcggccctgc ctcgcctgcg
    34561 ggctccggca gcttcttcag cttttcgagc tggttctgat gcctcacgcc gggaatgcgg
    34621 cggaagaaga cgggaagcgt cggcacggcc gcctcgcccc atgagcggat gtagtagccc
    34681 gcggcaaagc gggtcagcgc caaccactcc tcggtcatgg gcgcggagtc gcgcaggacg
    34741 gccttgatct gcctgaccct gttctccgcg aaatcggcgg ccgcccgaag ccccgccacg
    34801 atgcccacga cgttccgcgt gcgcaggccc acgacgacgc ggtcccccac tgcaacgagc
    34861 atgtccgccg gcacggcata gtcaagcagc ccgagccccg gcgtgtccac catcaccttt
    34921 gcataaagat cgcccgcttc ctttgtcatc gcctctggcc cgccccttga agaaatgtgg
    34981 ataatcttgt ggacaatttt ccgagctttt tcctaaaaac ccgtccaaat ccattgtccg
    35041 cgccactatc tgttcaatag gcatccccta acgggactgt cctgcagtcc attaaaagac
    35101 aaaggcgcca agacatcttt cgtcttgagc gcctttgcaa cgactcgcac agttgaaaaa
    35161 agctgtgcat aaccctatcg agaatcagtg atagcgtcgg ctatattcgt gaacttcgtc
    35221 gaccaggatc tgaacgtttt caatcggcgt gaactggttg atgccgtgtc cgagattgaa
    35281 gacatggccg ccattgccaa tcataccgaa gccgtccatg accttgcgtg cctcggcgcg
    35341 gattgtctcc tcggggccga agagggcgag cgggtccatg ttgccctgaa gggcgacctt
    35401 gtcctcaacg acgcgacgtg cacgggtcat gttgaccgtc cagtcgaggc cgaccgcatt
    35461 gcagccggag gccgcgattt cggagagcca ttcgccgccg cccttcgtga agacgatgga
    35521 cggaacaacc tcgccctcgt tttcgcgaac gaggctgtcg agcacctggc gggtgtagcg
    35581 cagagagaag cgctggaagt cgccgtcggc gagcatgccg ccccaggtgt cgaaaatctg
    35641 aacggcctga gcgcctgcgg cgatctgggc gttcagatac gtggcaacgg ccttggcgtt
    35701 gacgtcaagc acgcgctcga gcaggtcggg gcgctggtac atcatggtct tgaccgtacg
    35761 gaagtcgcgg ctgccgcggc cttcgatcat gtagcaggca agcgtgaagg gagagccgga
    35821 gaagccgatc agggggactc ggttgttgag ggccttgcgg atcgacgtca cggcgtccat
    35881 gacgtagcgg agctccttgt cggattcagg cacgtagagc ttggcaacgg cctcctcgct
    35941 ctgaacggga tggtcgaaca cgggaccctc gccggccacg aagtgcaggc cgaggcccat
    36001 ggcatcggga accgtcagaa tgtcggaaaa aaggatgacc gcgtcgagac cgtaacggtc
    36061 gatcggctgc agcgtcactt ccgtagcgta atcggggttc atggcgaggc ccatgaaact
    36121 gcccacgcgc gcacgggttt cgcaatactc cggcagatag cggccggcct gacgcatcag
    36181 ccacacgggg gtaaaatcgg ttttttcacg cttgagcgca cgcaaaaagc aatcgttgat
    36241 cggtttcatg gttcccactt agaaacggaa gaaatacaaa agagctagac atgcccgcca
    36301 acccagagtc ccctttcacg aacaccagct ggctgacgac cggcaagaac aacgcccccg
    36361 tcatcgcagt gcacgcgcaa ccgggcgcca agcggactgc cgtcgtcggc gttcacggcg
    36421 acaggctcaa gatcgccctg gcctctcctc ctgtggacgg caaggccaac gcgacgctga
    36481 tcaagtacct ctccaagggg ctgggcgtct ccaagagctc cgtgcgtctg ctgagcggcg
    36541 acacgagccg tgaaaaacga atagaagtcg tcggcctttc gaccgacgac cttctggaag
    36601 ccttgaagaa gctcttgtcc gacggttagc ggacgatgag gaccgggcgg tcgacctgat
    36661 gcaggacctt ctgggtttcg ctgcccagca ggaacgagcc gatggagccg agaccgcggc
    36721 tggccatcac gatgaaggac acgtcgtggc ggacggcgca ggccagcacg cccttccaga
    36781 tggcgtctgc atgctcggca accacttcgc acgggacgcc cttttcggca gccttgcggc
    36841 tgatggcggc cagccggtcg cggacggcgg catcctcggc ctcaaaacca ccggtcgggg
    36901 cctcgccgac gaccgaagtc atgccgatga gttcgccgcc gagctgcttc gtcagatcga
    36961 tggccgtatc aatcgccttg tcactcaaat tagacccgtc tgtagttacc agtaccttaa
    37021 cgccagccat gctgacctcc tgattgaatc aaataccttt gttctcaagc gcttggttac
    37081 gcatcaggta atcgtacctg catccagcag ccgactgacc tgcatccctg cgctttcttg
    37141 attttgaaca taaaaaaagg gcggtatttg aacaatacca ccctttttgg cagaaacaac
    37201 gttcgcttaa cgctgctgac gaatcttctt gattgccacg atctgagcgg ccagagcggc
    37261 catctcggct tcgaggcggg caatctcgat ttcgcccgtt gcattctgac gctgtgcgcg
    37321 ggcatcttca agagccttgg cagccttggc ctcgtcgagg tcattgctac ggacggccgt
    37381 atcagccagc acagtcacca gatcgggctg aacttcaagc acgccaccag ccacaaacac
    37441 ctgttccact tccttcttgc ccggcagatg aatgcggaca aaaccggggc ggatcagcgt
    37501 gatgaggggc acgtgcccag gcagaatacc caattcgcca gacttaccgg gcagcgaaac
    37561 gagctcggcg tcaccggaga agatggactc ttccgcgcta acgacgtcga ctttgatagt
    37621 agccatgtta gttgctccgt tgtcgggcgc ttgacagggc cggtcttgcg accggcctca
    37681 ggcaagcatt acttcagggt cttcgccttt tcaaaggctt cgtcgatggt accgaccatg
    37741 tagaaggcct gttcgggaag ttcgtcgcat tcgccttcaa caatcatctt gaagccgcgg
    37801 atcgtttcct tgagcggaac gtacttgccg ggagcaccgg taaacacttc agcaacgtgg
    37861 aacggctggg agagatagtt ctggatcttg cgagcgcgca taaccgtcag cttgtcttcc
    37921 ggagcgagtt cgtccatacc gagaatggcg atgatgtcgc ggagttcctt gtagcgctgg
    37981 agcgtttcct gaacacgacg gcagatggag tagtgctctt cgccgatgat gttcgggtcg
    38041 acctggcggg acgtggagtc aagcgggtca acggccgggt agataccaag ggcagcgatg
    38101 tcacgagaca gcacgaccgt ggcgtccaag tggccgaagg tggtagccgg agacgggtcg
    38161 gtcaagtcgt cagcaggcac gtaaacggcc tgaatggacg taatggaacc ggtcttcgtg
    38221 gaagcaatac gttcctgaag cttacccatt tcttcagcaa gcgtcggctg gtagcccacg
    38281 gcggacggca tacggccgag cagagcggac acttccgtac cggcaagggt ataacggtag
    38341 atgttgtcga tgaagagcag gatgtcgcgg ccttcgtcac ggaaggcttc ggccatcgta
    38401 aggcccgtca gagcaacgcg gagacggttt cccgggggtt cgttcatctg accgaacacc
    38461 atggccacct tgtcgagaac gtgggactct tccatttcgt ggtagaagtc gttaccttca
    38521 cgggtacgtt caccgacgcc ggcaaacacg gagtaaccac cgtactgctt ggcgatgttg
    38581 ttgatgagct ccatcatgtt gacggtcttg cccacaccgg caccgccgaa gaggccgacc
    38641 ttgccgccct tggcgaacgg gcagataagg tcaataacct taatgccggt ttcgagaagt
    38701 tcaacggacg gggagagttc gtcgaacttc ggggccggac gatgaatcgg gcgaacttct
    38761 tcagcgccga tgtcgccgca gtcgtcgatc gggcgaccga gcacgtccat aatgcggccc
    38821 agggtcttgg gaccaaccgg gacggagatt tcatggccgg tgctcttgac cttcatgccg
    38881 cgctgcaggc cttcagaaga gcccatggca atggtacgca ccacgccgtc gcccaactgc
    38941 tgctgcactt cgaaggtcag gccctcttca gccaggggat tgctttcatc cttttcaagg
    39001 acgagagctt cgtaaacctt aggcatggca tcgcgggcga actcaatgtc aaccacggcg
    39061 ccaatgacct gaacgatctg tccgatactc atgggttttc ctcagttatc gatacttaag
    39121 acacggcggc ggcaccaccg acgatttcgg tgatttcctt cgtaatgccg gcctgacggg
    39181 tcttgttata aacgagctgc agctcttcga tgagcttctt ggcgttgtcc gaagcggcct
    39241 tcatggccac catgcgggcg ctctgttcag aagccatatt ttctgcaact gcttggtaaa
    39301 tcaaagcttc gacgtaacgc ttcagaagcg cgtcgagaac ggtttcggcg tcgggctcgt
    39361 agagatagtc ccacgagtga tcacgcttgt cagccgcatc aagatggctg tccgtaagcg
    39421 gcagaatctg ctcgaccacg ggcacctgct tcatggcgtt aacaaaacgg gaataggcca
    39481 gatgcacaga atccaccttg ccttcgacgt aagcctgaat ctggatgcgg atggcaccga
    39541 tcagacggtc gaggttcggg cggtcgccaa gctggacggc ctggctaacc acattggcct
    39601 gaaggcgctg gaggaagcct aaccccttgt taccgagagc ggtggcttca atggagtagc
    39661 cttccgcgct ccagtccttg atcttgtgca gaacaaggcg ctgaatgttg gtattcaggg
    39721 ggccggcaag ccccttgtcg gtcgtcacga gaatgagacc aatcttcttg gtgccttcat
    39781 gcttaacgag gaacggatgc ttgtattcgc tgttcgcacg ggcaagatgc gagcagatca
    39841 ctcgaatctt gtcaccgtac ggacgggccg caaacatccg gtcctgcgcc ttgcgcatct
    39901 tcgaggccgc aaccatctcc atcgccttag tgatcttgcg cgtgttttgc acgctcttga
    39961 tctttccgcg aatttccttt gtactgggca tgggatatgg gtcctcttgg atttgttaac
    40021 gcttagtaag caccgctctt cttgaacgac tcgatggcag ccttgagctg ggcatcgacg
    40081 tccttggaga gttccttctt ggcttcgatc gtttcgatca ggtcagcatg gtgcgtcttg
    40141 aggtattcac gcatgccgcg ttcgacacga agcgcgtcag caacagccac gtcgtcgtag
    40201 gcacccatgt tcacggcgta aagcacgaga gcttcttccc aaacctgaag cggctggtac
    40261 tgcggctgct tcatgagttc ggtaacgaca cgaccgcgtt cgagctgctt gcgggtaaca
    40321 tcgtcgaggt cagaggcgaa ctgagcaaag gcagcgagtt cacggtactg agccagggca
    40381 agacggacac caccgccgag cttcttgatg atcttcgtct gagcggcacc accgactcgg
    40441 gacacggaaa tacccgggtt gatggcggga cggataccgg cgttgaagag gtcggtttca
    40501 aggaagatct gaccgtcggt aatggaaatc acgttggtcg gaacgaaggc ggtaacgtcg
    40561 ccagcctgcg tttcaatgat cggcagagcc gtcatggaac cggtcttgcc cgtaaccttg
    40621 ccttccgtga agcgttcgac gtaaaccggg ttcacgcgag cggcacgttc gagcagacgg
    40681 ctgtgcaggt agaagacgtc gccagggtag gcttcgcgtc ccggcggacg gcggagcagc
    40741 agggaaatct gacggtaggc ccaagcctgc ttcgtcaagt cgtcatagac gatcagggag
    40801 tcttcaccgc ggtcgcggaa gtattcaccc atcgtggcgc cggcgtacgg agcgatgtac
    40861 tgcatggcgg cggattcgga agccgtggcg gcaacgacga tggtgtattc catggcgccg
    40921 ctttcttcga gcttgcgaac gacgttggcg atggtggagg ccttctggcc gatggcaacg
    40981 tacacgcagg taaggttctt gcccttctgg ttgatgatcg tgtcgatggc gacggcggtc
    41041 ttaccacact ggcggtcgcc aatgatgagt tcgcgctggc cgcggccgat cgggatcatg
    41101 gagtcaacgg acttcaagcc cgtctgaacc ggctgggaaa cggactggcg gtcgataacg
    41161 ccgggagcaa ccttttcgac gacgtcgaag agttccgtgt tgatcggacc cttgccgtcg
    41221 atcggctgac cgagagcgtt cacaacgcgg ccgataagtt ccggaccaac gggaacggaa
    41281 agaatcttgc cggtcgtctt gaccaggtcg ccttcggaaa tatgttcgta ggaaccgaga
    41341 ataacggcac cgacggagtc acgttcgagg ttgagggcga ggccatacgt attgccgggg
    41401 aattccagca tttcgccctg catcacgtcg ctaagaccgt gaacacggca gataccgtcg
    41461 gtcaccgaca cgacggtgcc ctgggtacga agatctgcgg aaacgccgag gccttcgata
    41521 cgcttcttca gcagatcgct gatttcactg ggattgagtt gcatcttaca gctccgaatg
    41581 tttttaagcg gtgagcgtcg tcttcatctg agcgagacga gcgcgaatcg aaccgtcaag
    41641 aagtttgtcg ccgacacgaa tgcaaacgcc gccgatcagt tcgggattga tggaaacgat
    41701 cgggtggagt ttgaggccgg ggaacttctt ggcaagcgac tcaagcaggc tgtcgagctc
    41761 agcggcgcta agctcaaagg ccgactcgat gtaggcatcg gcaatgcctt cggaagaatt
    41821 cttcagctca cgaaactgac gggcgatctc cgggaaagct tccagacgac cgttttcgat
    41881 gaccacgcgc agcatgttcg cgacttcctt cgggaggttg ttgccgcaaa gttccgcaat
    41941 caggtcgcag agttgatcgt cagtcagctt cggatcgcca accagcgtgg cgacttcagg
    42001 attcttagtc agggaagcga gagcttcgat gacttcaaga tcctgggcca catcttcagc
    42061 gccggcatta cggtctttca aagccttcat caggccttgt gcgtaggggc gcgcaatcgt
    42121 cgaaagttca gccatagtta gagcttcgcc ttcaattgtt caagcatggc ggcgtgaacg
    42181 gtcttgtcga cctcacgggc caggatctgc tcagcaccgg caacggcaag agcagccact
    42241 tcgttacgaa gcgcttcacg agcacgctgc atttcctgag cagcttcagc acgagcattc
    42301 tcgatgatgc ggtcggcttc gacctgagcc tgagctttgg cttcatcgac gatactctga
    42361 gcacgctttt caccgtcaga aacaatggtg gtggcacggg cgcgggcgtc ggcttcgatg
    42421 gccttgccct gctcggtcgc gatagccaga gccttctcac ccttgttagc ggcagagaga
    42481 ccatcggcga tcttcttctg acgctcttca atcgcgtgga tcagcggcgg ccagatgtac
    42541 ttcatggtga tccaggcacc aaggaagaac acgaccatct gtaaaaacag agaggcatta
    42601 atgttcatta ttgaaccccg ttacgttgac cgccctctcg cccttctagc gttcaagcag
    42661 ggcagtcaac ggcgtcatta aaagaaaaga cgttttattt tcgcggcacc aaattagccg
    42721 acgaacgggt tggcaaaggc gaacatcatg gcgataccga caccgataag gaaggcagcg
    42781 tcgataagac cggcgagaag gaacatcttc gtctggagcg gttccatcag ctcgggctgg
    42841 cgggcggcgg attcgaggta cttggcaccc atgatggcga tgccgagaca ggcaccgatg
    42901 gcgccgagac cgatgatgag accgcaagcg agagcgacga gagcgacgtt ggtcatttta
    42961 aaaaactcct gaatttaaaa agagggagta aaggaaaaaa gagattagtg gctgctgtgg
    43021 gcctgaccga tgtagacgag cgtcaacatc atgaagatga aggcctgaag cagaacaatc
    43081 aggacatgga aaagccacca gaccaaacct gcgaagactt gaccaatacc agcactaaca
    43141 gtcccaatgg aactgagttc gagagcgaaa ccgccgagaa gcgccagcag gaagaacagc
    43201 aattcaccag catacatgtt gccgaagagt cgcatgccga gcgagacgaa cttggcgagg
    43261 tactcaatga tgttcaacag gaagttgaac ggccagagag cgggatgact gccgaaaggc
    43321 gcagtaaaca gttcatgcac gaagccgccg aggcccttga ccttcacgcc gtagtagaag
    43381 agaaggagga gcacgcccaa cgacatgccg agcgggccgt tgaggtcagc agtcggcagc
    43441 ggacggagat gatcgaggcc gatcagacca gcaatcgcgg ggaacaggtc aaccggaatc
    43501 aagtcgatgg agttcatcaa cgcgacccag cagaacacgg ttaaagccag cggagccacg
    43561 aaggtgcggt cgccgtgcac gatgttcttg gcctggttgt tgaccatttc caccagcatt
    43621 tcgactgcac actgcatctt tccggggaca ccggaagtag ccttcttagc accggctcgc
    43681 agaaggaaga acatgacagc cagcatcaac acggagaaga agatggtgtc gtagttcacg
    43741 atagaaaaat caacaacgga cccctgatgc gcggaagcca ggtgggccat gtgatgttta
    43801 atatactcag tagcggtaag ggcttctgct gccatggata ccgatcctta atgtctcttg
    43861 aacagcacga tgaagtaact gttcgccacc gcaattatcg tgaagataaa ggcgggccaa
    43921 tgaaggtttt cgtagtaccc gaccatcagc cccaaaagaa gcacgacaac caggatcttg
    43981 acaaactccc ctccgagcac tgcgacagca cttgggctgt acgccgtcaa aaatttcggc
    44041 agcaccaaaa acaacgcgag gagcgtcgtc gggaccgcgt aagcgagccc cgccatcagt
    44101 gccgtgtagg ctgcatcctg ccctacgaaa aaagcagaaa caacagttac aagaaatacg
    44161 atgacatact gcgcaaggga aatgcgcatc atatcagaaa tggcgtacat acttgcggtt
    44221 gttcgttggg ttttcagtcg gatcgaattc taagttttgt cttaaataca tacaactcac
    44281 caaacgggga acactcctac ctcgttatgg gtatgctcta tagaatatat ctattaagca
    44341 cgaagtcgcc taagcccttc gacttaatgg ccctgctgtt ggatccggtg tacgatgccc
    44401 tcgagctggt cgagattggt aaattcaatc acgatcctgc ccttgccctt cgagttggcg
    44461 gagagcttca cgacggcacc gagcgtttcg gcgagcgact cctcgagtcg aatgtcgtcg
    44521 cgggtcttga ccacgaactt ctgacgaacg ggttgctttt ccttcatccg acggatcaag
    44581 tcttcagttt gacgcaccga cagcgctttg gcaaccactt ctttcgcggc cagcacctgt
    44641 tcggcggcat caagcgcgag aagcgcacgc gcatgtccca tctcgagctt gcgctccttc
    44701 agcatggcct tcacttcagg cgtcagcgtc agaaggcgaa gaaggttcgt cgttgcggtg
    44761 cgcgaacggc cgacggcttc ggccgcctgt tcatgcgtgt agtcgcactc gtcaatgagg
    44821 cgctggatgc ctgccgcctc ctcgagagga ttcaggtcct cacgctgcat gttctcgatg
    44881 agcgccatgg cgagtgcctg ggtctgatcc accgtgcgga tgatgacggg aacctcttcg
    44941 aggcccgcca tgcgggcggc gcggaagcgg cgctcgccgg cgatgatttc gtagccctcg
    45001 tccgcaggac ggacgatgat cgggctgatg atgccctgct ggcgaatgga gtcagccagc
    45061 tcctgaagac cctcttccgt catggtgctg cgcggctgag tgctgccagg gcgcagctcg
    45121 tcaagcttca ccgtttcaag gcgcttgtca tcaccgtcct cttcggagag gccgctcacg
    45181 tcgaagtcgt cataaatggc gcccagaccg cggccgaggc ctccaagttt ctttgccgtc
    45241 acgttttctc ccgttgaatt acttgtgctt cttttccatg cgctcgatga gttcttccgc
    45301 acaggccatg taggccttgg cccccttgct cgagcggtcg tagacgacgc cgggcaggcc
    45361 gtagctcggc gcctcggcaa gcctcacgtt gcgcggaatg atggtcttga agaccttgtc
    45421 gccgaaatgc tcctcaagct gcgtgctcac ctgctgctgg agcgtgatgc gcgtgtcgaa
    45481 catcacgcgc agaagcgcca cgatgcgcag gttcttgttc ttctcggcac gaacgcgctt
    45541 gacgctgccc acgaggtcgg taagcccctc gagcgcatag tattcgcact gcatcggaat
    45601 gatgacgccg tcagccgcgc acagcgcgtt gagcgtcagc atcgacaggc tcggcggaca
    45661 gtcgatgagc acatagtcgt acttgtcgag ctcgggctcg agcgccttct tgaggcgcac
    45721 gtctcgatga tcaagcccca cgagctccac ctcggcaccc gccagctcgc gattggcagg
    45781 cagaacgtca aagccgccct cagccgaatg gcagacagcc tgctcgaagc tcgcgatgcc
    45841 gagcagcacc tcgtagatcg aacccttcag gtcgcgcttt tcgatgccgc agcccatcgt
    45901 gccgttgccc tgggggtcaa ggtcgatgaa gagcaccctg cgtcccttca aggcgagggc
    45961 gcctgcaaga ttgacggcgg tcgtcgtctt gccgacccct cctttctgat tagcgataca
    46021 aaagatttgc gccataagtt cgtcgtccaa acgtcgtgcg gtccgctgac cttcggttgt
    46081 ctcaaatgtc taatagtaat gcgcggccgt catccgcaag aatgctcact tgaccgcagc
    46141 tgcggcggct gtcgcctgcg cgctgagctc caaccagagt tcggagaaac cctgccagaa
    46201 aatggcgacg cccagacaga tcaggatgaa ggcgaagatt cgcgccagcg cgtctgcgcc
    46261 cgccacgccg accgccttgt tcagacggtc gctgtaacga tagcagcccc agatgaccgc
    46321 caccgttgca accgtcgcca caaacgtgcc ggcgatgtgc ccgaggtctc gcggcagact
    46381 ggtgctcacg gccacggaca cggcaatcgc accgggtccc gtcgtgagcg gcagcgtaaa
    46441 ggggtagaag gccatggact tgagcttcgc gcgggacagc gcggggcgct cctccgtcgt
    46501 ctcctcctgc gtcggcgcat tcagggcagt ccagcctgcg gcgaaaagca cgcagccgcc
    46561 cgccattcga agcacgccga cggaaatgcc gaagaagctg agaatcaggt gtccgcacgt
    46621 caggcacacc agcagaatga tcaggcagta cagggcgatc ctgttggcaa cgaacgcacg
    46681 atccctgtcc gacaggctgg ttgtcagcgt acagaagaac attgctccgc tgaacggatt
    46741 gatgacgggc aggaatgtcg agaatgcgag tacgaaggca ctgacgaaag cgctggtcat
    46801 gacagtctcc taaatctcta gttgacgggc ttcatcacca ccagatggcg agcctctcca
    46861 agcccgggaa cctccatggg aagcacctct gcgacggatg cgtcggcagg cagacaactg
    46921 atttcatcct cgggcacggc gcccttcatg gccagccaga gaccgtccgg cttgatgagg
    46981 tggcgcgtca ggtccacaaa gtcctgaaga ttcgcaaagg ccctggacgt gatcaggtcg
    47041 tactggcccg aaagacgttc gattcgcgca tggcgggtcg aatagttcct gagtcccagc
    47101 tcgatggcgg cctgagtgag aaaggccgtc ttcttgctga cggcatcaac cgccgtcacg
    47161 cgaagatcag gcctgagaat ggcaagcggg atcgacggca ggccgcctcc gctgccgacg
    47221 tcgagcacgt tcaccgcatc agggccgaga cgtttcacga acggcacgag cgccgccgag
    47281 tccagaagat gatgcgtcag gatctcgtca tcgcggcgaa tggcggtcag gttgtagacc
    47341 ttgttccact tctggagcag gcccgcaaag cgcatgagtt ggtccacctg gtcgacggag
    47401 gcctcaagac caagcgtcgc gaggccttcc tccaggcgtg gctgaaaatc aaacattgcc
    47461 tttttccttg gcttgagtgc ccttgtcctt cacatagtag tagcggcgct tcaaatgcac
    47521 cagcagcagc gagaccgcag ccggcgtcac gccggagatg cgggaggcct gcccgatcgt
    47581 ctcgggactg atcgacttga gcttctgtcg aacctcgaag ctgagccccg gcacgctgtc
    47641 gtagtcgagc tcggcgggaa tcttcagcgt ctcgttggcc agcgtgcgct cgatctccgc
    47701 cttctgacga gagatgtagc ctgaatactt tacttccgtt tcggcctgga acagctcgtc
    47761 atccgagaag tcctccagag gcaggacggg agcaccgtcc gtcttcgtca tgcccgcaac
    47821 ggccttgaga gtgacgccgg ggcgggtggc cagagcgtga agcgagtatt cgcgctccat
    47881 gtcggtgccg agcacgcggt tcatctcctc ggaagtgaag agcttcggat tgacccacgt
    47941 gtccttcagg ctctgcatca ggcgggcgac gttctcctgc ttgcggcaga agtaggccca
    48001 gcgttcgtca tcgacgaggc cgagacgtcg cccttcctcc gtcaggcgca cgtcggcgtt
    48061 gtcctcacgc aggctcagac ggtattcggc gcggctggta aacatgcggt agggctccgt
    48121 cacgccgcga gtcgtcagat cgtcgaccat cacgccgaga taggcctgat cgcggcgggc
    48181 cgtccaggag ccttcgccgc gcgcgtagag cgcggcattg agaccggcga gaagcccctg
    48241 tgcggcggcc tcttcgtaac ccgtcgtgcc gttgatctga ccggcaaaga agaggccggg
    48301 catggccttc gtctccatgc tggtcttgag gccgcgcgga tcgtagaagt cgtattcaat
    48361 cgcatagccc ggacgcagca gatgcgcgtg ctcaaggccg ggcatgttgt ggacgatgtc
    48421 gagctggacg tcgaagggca ggctcgttga gatgccgttc ggataccact cgttgacatg
    48481 cagaccttcg ggctcaagga acacctgatg gctgtccttg tcggcgaagc gcttcacctt
    48541 gtcctcgatg gacggacagt agcgcgggcc gatgccgtgg atgacgcccg tgtacatggg
    48601 cgagcggtcg aggttggcca gaatgatgtc atgaactttc tggttcgtgc gcgtgatcca
    48661 gcagggcacc tgcctcggat gttccggagc gggcttgagc agcgaaaact gcggcacggg
    48721 atcaaggtca ccccactgct cctcgcactt gctgaagtcg atcgtacggc cgtcgattcg
    48781 ggcgggcgtg cccgtcttga gacggccctg aggcattccg agctccttga ggcgctccgc
    48841 cagacggacg ctcgcgggat cgccgacgcg gcccgcctga tagtgctcaa ggccgatgtg
    48901 caccatgccg ttgaggaagg tgcccgcgca aagcacgacg gcctgcgcgc tgaaacagaa
    48961 gcccgtctgg gtcacgatgc ccgcaacgcg gccgttttcg acgatcaggt catcaacggc
    49021 ctgctggaag acgctgaggt tctcctggcc ttcaatgcgc tcgcgcatgg cgcggcggta
    49081 aagctgacgg tcgatctggg cgcgggtggc ctgaacggcg gcacccttgg agccgttgag
    49141 aatgcggaac tgaatgccgg cctcgtcagt cacgacaccc atggcgccgc ccatggcgtc
    49201 aagttccttg acgagatgac ccttgccgat gccgccgatg gacggattgc aggagagttg
    49261 gccgatcgtt tcaacgttgt gcgtcacgag cagcgtgcgg cggcccatgc gcgcggcggc
    49321 aagcgcggct tccgcgcctg catggccgca gccgaccacg atgacctcaa atttttcggg
    49381 gtacagcatt tattttccaa tacagaactt actaaaaatc atgccgagga tgtcttcggt
    49441 cgtcgtttcg ccgaggatct ctccaagcgc gcggctcgcg aggcgaagct cttccgccac
    49501 cagttcgggc atgggatcgc agtcgatgag aaggagggcg cgggagacat gctccgccgc
    49561 cgtcttcagg cactgcagat ggcgttcgcg cgccatgaag acgccgtccg tcgtcgccgc
    49621 catgccgacc acctcgagaa gacgggcctt gagttcggac aggccgactc cctgtttggc
    49681 ggaaatggcg atctcgccgc cggaggcagc cgactcgcaa agatcgacct tgttgttcac
    49741 ggtgatgagg ggcacgccgt ggcggacctc cttcagaacc ttgtccaaaa cctccgtgtc
    49801 gtccctgatg aggccggagg cgtccttgag atgcaacacg acgtccgcct tgcggatctc
    49861 gaccaacgtg cgttcgatgc ccttgctttc gacgacgtcg tccgtcgtgc gaatgcctgc
    49921 cgtgtccacc atcttgacgg gcacgccatt gagcgaaacc cagtgctcga tgcggtcgcg
    49981 ggtcgtgccg gggatgtcgg tgacgatggc cacctcctct tgggcaagcg cattgagcag
    50041 gctcgacttg ccgacattgg gactgcccac gatggccacg gtcacgcctt cacgcagcac
    50101 ctgcccctga cgagcgttct ccagaatggc gcggatgccc gtttcggcct gaacaaggcg
    50161 cgtgcggacc ttttccgaac cgatgacgtc gatgtcctct tctgggaagt cgagcgtggc
    50221 ctcgatgaag gcgcgaagct ccgtgatgtt gccgtcgacg tcgcggacgg cattggagaa
    50281 ctcgccactc aaagagcgtc ccgccgcctg aacggcggat tcgctcacgg catcaatcag
    50341 atcggccaca gcctccgcct gagtcaggtc cattcggccg ttcatgaatg cgcggcgggt
    50401 gaattcgccg ggctcggcca tgcgaagccc gagatcacgg cacttctcaa ggcaggtgcg
    50461 catgatgagg ctcagaagca cgggaccgcc gtgcgcctga atttcgagga cggactcgcc
    50521 cgtgtagctg tggggagcgg gaaagagcag cacgattgcg cggtcaagca ccccgccgtc
    50581 cgttccgagg aagggcagga gatgggcgta gcgcgccttc agggtgcagt tctccccgaa
    50641 caggcgcccg acgatctggg cttcgagcgc cttgccgaag gacaggcgca gaatgccgat
    50701 gccgccgcgg cccgcggccg tcgcaacggc gatgatggga tctttgtcgc tgtggtgcat
    50761 aaagctcttt ccgagggttg ccgacgtcag gatcggcaga ttgtggagat tgtaatgccc
    50821 gccccgatct ctttggtata cgatggatgg gtttgtgtat tcaggtccct ttgaacccat
    50881 gtccctgcca cgtcctccca accgcaagcc ctttttcgga ccgacgctga tcggcgcctg
    50941 caccggcatg attctgtgca ttcccctcgc ggcctacacc gccgccgcgc tcggggacga
    51001 ctacaacacg cgggccctca tctactgcgg ctttcttctc tggtgcatcg tcggcgccgt
    51061 cgtgctcttc atgaagacgt ggcagtcgga aaaccagaag atctcgcctg cgaaatgcct
    51121 gctctggtgc gcctctctct ggctctggcc tcttctgctg ctgccgggac tcttcaggaa
    51181 gcgcagctga acctgcgctc aaacaaaaag ggatgccttc taagggcacc ccttttttgt
    51241 ttgtcttcaa agaagcacta gtgctttagc tctgcagacg cttggcgcgc tcggctgcga
    51301 tggtcttgtt gatccaccac tgctggagaa tcgacagaac gttgttcgta agccagtaga
    51361 gcacgagacc ggaggcgaac acgaagaaca tcaccgagaa gacgatcggc atgatgtaca
    51421 tcatgcgggc ctgcgtcgga tcagtgggct tcgggttgag caggatctgc aggaacatgg
    51481 tcacggccat gatcgcagga agcacgagcc aagggtcagg catcgcaagg tcatggaccc
    51541 agaggaccca cggagcaccg cgaagctcaa cggaaccctg aagcacccag tagagcgcga
    51601 ggaacacggg aatctgcagc atgatcggga ggcagccgcc gaccggattg atcttttccg
    51661 tgcggtagag ctccatcatg gcctggttga ggcgctgctt gtcgtccttg tagcgatcct
    51721 gcagctgctt catacgcggc gtgacttcct tcatgcgggc catcgaacga tagccggcgg
    51781 ccgaaaccgg atagagaatc gccttcacga tgcaggtcag aagcacgatg gcccagcccc
    51841 agttgccgac gatgccgtag aggaaggaca ggagccagta gatcggcttg gcaaggaacg
    51901 tgagccagcc gtagtcgacc acgagctcaa ggcccggagc gatctgttca aggcggtcct
    51961 gatcctgcgg gccggagtag agcacggcct tgtcggtcac ggacttgccg gactcgatgg
    52021 tgccgagctt cacgagcgtg ccgacggagt agaggttctt ggagacctca cgggcatagt
    52081 tctcgcgctc ctcaccgttg gcgggaaccc aagcgctcac gaagtggtgc tggaccatgg
    52141 cgagccagcc gttgttggtg aacttctcga aggaggcgct gttatcggcg atgtcgccga
    52201 actcaagctt ctggaacttc tcttcgtcgg agtagaaggc gggacccgtg tacgtggagt
    52261 acatggaact ttcgccttca ggcttgccgc cgtcgcgggt gagctgatag taggcggacg
    52321 gctgaatggc ggcgccgctg ttgttgacga cttcggtcga aacgtcgatg ccgtagtggc
    52381 cgcggttgaa cgtgtaggtc ttcttgaccg tcacgccgct cttggtggct tcaaagatca
    52441 cgtcaagctt gtcggcgtcg cccatgctga gcttttggct gacgagcttg tactgatcct
    52501 tgtgattcgg gaagttgccg ccgacaaggc cgctctgggc gacgtaggtg cggttcttcg
    52561 tgatctcaag aagatcgacg ttgccgaggt tctcggctgg cttgcggtcg aggatgagac
    52621 cggcgaggcc cacttcggtc cagtcggcct gacgcgggat cagcgtcatt tcggtgccga
    52681 cgatgacggc gccctcgcgg tcgaaggtga ccttcatgcg atcggtttca acgacgacag
    52741 gatccttgac ggcgacagcg gacggaaccg tggcgtcaat gtccttgcca gtcgggacgc
    52801 tagaggctgc gtcagcaacg gcctgctgtt caacggcctc cgtatggaaa aaggacttct
    52861 cgcccttgta gacctgatag ttgtcccaca gcaggaagca ggaggtaaaa agaatcgccc
    52921 acaaaagagc gcgttgcatg tcctttccca tttttatcca ctacttcttt tggatgttga
    52981 aaagatccgt agatcccttg tcttcttgtt cttccggcct gcactctctg caccagcagc
    53041 gccaacggaa cttcgccggc accggatcca gcccgcgccc tccaagagga tgacagcgca
    53101 gcagacggta gatggtcaga taggttccct tgacggcacc gtacttttcg agtgcctcaa
    53161 tggcatagtt tgaacacgtg ggaacgaatc ggcaactcct tcccacccac ggagacaggg
    53221 tcagctgata gagcctgacc agcatccgca gaaacttcgc caccaccgtc actcgccccg
    53281 ggtctgcgca agctgcttcg gaagacgctt cagaagcgcc tccatgagcg accgcgcatc
    53341 agtcctcagg gacagcttca gggcgttgac gccctgatcg atgccgggga tctccttcag
    53401 gggaaccctg agccttaaag aaacgtcaag ccccacgcct tgctcgccga gaagcgccgc
    53461 aagatttggg gcttgctttc ttgcagcctc cctcaaggtt ctcttgacca gtgcacggtc
    53521 cacggagcga tgggcgttgc gctttccgac ggtgatgccg aagcgaagcc tgcccggctc
    53581 ctcagggaac ttcattgcgt tgatcgaaaa gtacttcgac gtgagtcgga aggtattttc
    53641 gttccgcgta tgcacaagca ccccatagtc cgcgctcttg atgatgcgca aactacgggg
    53701 aaacttaagg ggtgccgctg tgtagctgga cacggcgatc gttaccggcc gtgaacgagc
    53761 cgattagaga gccagggtgt gacggccctt agcgcgacgg cgagcgagaa cgcgacgacc
    53821 gcccttcgtg cggctgcgaa ccaggaagcc gtgcgtacga gcacgcttga ccttggaagg
    53881 ctgataggta cgtttggtag ccatgattaa tcctcaatag gcatgaacct cggcgcacgt
    53941 cggtgcagca cgaggaaaaa acaacagggg tatgccagcc acacagtccc atgtcaatcg
    54001 ttctggaaac gggctagcca aatgagtctc cgcaagccgg cgcttttgtt ctgtacggtg
    54061 ccgcccgcgg tgccgccggc gaccccgcaa tcatcgagat tgccgagtta cgaaaagtta
    54121 gatattagaa ctcaatgagg ggggatttgt caaacgaaaa ccacaaaaaa tcttgtcctg
    54181 tcatgaagtt cggtaggctt cccctcaggc gaaggctcga aaaacacaac atctgggggt
    54241 cgggagccga caaaccacat tgggttgtgt aaatcttttc tccacgcttt ttcaggaaag
    54301 cccaagttgt tgagaggcaa gggaaatctt atccacaccg aattgtggat aagttatcct
    54361 caagcaaagt tatccacgga caaatcggct aaaataatat attcgtcttc cttgccccgc
    54421 cctgcccggc aggccgccca ggcaagcctt caatctacat ctaggcccgt catgaacaac
    54481 ttctggcaag actgtctcgc gcagatcaaa gcgatgaagc ttcctccgat gatcattacc
    54541 aattggttcg aaccgattga gctggtgcgg tgggacagcg aacgcggcga catggtcctt
    54601 gaagcgcccg ccagcaaggt gcagacgatc cgcgcgac
//
LOCUS       DBMDTI010000002        39668 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_400_length_39668_cov_4.012814, whole genome shotgun sequence.
ACCESSION   DBMDTI010000002 DBMDTI010000000
VERSION     DBMDTI010000002.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 39668)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 39668)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39668
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_400_length_39668_cov_4.012814"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="ACZPB6_00285"
     CDS             <1..576
                     /locus_tag="ACZPB6_00285"
                     /inference="COORDINATES: protein motif:HMM:NF016228.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HIR1182949.1"
                     /translation="EAHPRQAGVYGVTKHEGERLLEESGVSGAIVRAGWLHSGERDFV
                     NAILSAAAEGRALYVTASQIGTPTSTEALARWTWDALPELTPVDRLTTVHYVEEGGWI
                     SRADMAVHALEAAEDRAIRLGEFKLAIRFRKAREGMRRTDADDGFRPLNCRLSSLKKD
                     EFPRTERWMEGVDKSVDIVVEAKKSLWITQK"
     gene            complement(675..1670)
                     /gene="galE"
                     /locus_tag="ACZPB6_00290"
     CDS             complement(675..1670)
                     /gene="galE"
                     /locus_tag="ACZPB6_00290"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR1182950.1"
                     /translation="MNILLTGGMGFIGSHTAVVLIEAGHDVVLFDNLSNADRSVLDRI
                     ETITGRRPVFIEGDIRDAAAMEKALDGVDAVIHFAGLKAVGESVSKPVEYYDNNVNGT
                     LVLLAAMRRKGVNRIIFSSSSTVYGTPETLPLTEDCPTGEPTNPYGRSKLHIEQILAD
                     VCRAYPEFSAVCLRYFNPIGAHPSGLIGEDPNGIPNNLLPYVARVAHGTLPRLRVFEH
                     DYPTPDGTGVRDFIHVMDLADGHAKAVPYIMNHTGWIAINLGCGRGYSVLEVVKAFEK
                     ASDRSVPYELVDRREGDIAANWCDPARAERLLGWKASRTIDDMCRDAWNFESNKR"
     gene            1822..2217
                     /gene="ruvX"
                     /locus_tag="ACZPB6_00295"
     CDS             1822..2217
                     /gene="ruvX"
                     /locus_tag="ACZPB6_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR1182951.1"
                     /translation="MQEGTVLCFDFGLARTGVASGNTLTRSAQPVTIIHARTNDERWK
                     AVEALVKEWEPVLLVVGVPRHGDGTDCDLTPRCERFARQLGGRFRLPVRTVDERFSSV
                     MVEHGADKIDDEAAAVILQQYFDEQDAVA"
     gene            2229..3080
                     /locus_tag="ACZPB6_00300"
     CDS             2229..3080
                     /locus_tag="ACZPB6_00300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1182952.1"
                     /translation="MRYVIGCGRMAAVGIMILVILFILLVLFPLVGRKGRGRCVRRVC
                     RVLMRVLGVRMTVRGAVPVAQSAECGVNGEGPGYMVCANHVSFIDIFILDAVLPCRFV
                     AKKEIASWPVFGFIARGTGTLFIDRSRKRAVLEIADAMAGAINEGTNVLFFPEGTTGN
                     GLELLPFHPNLFEAAVRSGAQILPVTLRYTLDGKPSGLVSYAGDVALFTVLKRILFTP
                     GLGVEVTVLDPVSVDGKTRQALCLEASALMSGALGVSDVTAEREEARRRRLQAMSETK
                     AEAEKTV"
     gene            complement(3088..3912)
                     /locus_tag="ACZPB6_00305"
     CDS             complement(3088..3912)
                     /locus_tag="ACZPB6_00305"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HIR1182953.1"
                     /translation="MVTNPYFAIGDLQGCLESLDGLLEQLPEDAPLIFLGDLVNRGPE
                     SLATLRRVAGLGSRARCLLGNHDLHLLAVAAGASKVHKKDTIGEIFEAPDADELIEWL
                     RHQSIMIETEDAVFVHAGVHPLWTLEKARGLAREAEAVLSGPDWKAMLQQMYGNSDWD
                     DGLKGGERMQAILNCFTRMRFVDKKTAELDFKQKEDIGSAPKHLIPWFEYSKRPMAGT
                     PICFGHWSMLGLIRRDDIVAIDTGCLWGGKLTAVRFPDRRIYQEDCPCWADPFKYK"
     gene            4020..4859
                     /gene="galU"
                     /locus_tag="ACZPB6_00310"
     CDS             4020..4859
                     /gene="galU"
                     /locus_tag="ACZPB6_00310"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR1182954.1"
                     /translation="MKPVRKVVFPVNGLGTRFLPATKAMPKEMLPVVDMPLIQYAVAE
                     AAAAGITEMIFVTGRNKRAIEDHFDSYPELERELEEKGKDELLELVRGIVPAGVKCIY
                     IRQPKALGLGHAVLCAKPVVGDEPFGVMLADDLVDAPSSCIGQLTAVREARGGGSVIA
                     VQDVAPEATNKYGIVSVDDTSLQTSQMRGIVEKPDPKVAPSRMAVIGRYVFEPEVFDY
                     LEKVTVGVGGEIQLTDGIASSLDTVPTYAHRFEGTRFDCGSKQGFLDATIHFARKRGF
                     KIN"
     gene            complement(4943..5437)
                     /gene="rfaE2"
                     /locus_tag="ACZPB6_00315"
     CDS             complement(4943..5437)
                     /gene="rfaE2"
                     /locus_tag="ACZPB6_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HIR1182955.1"
                     /translation="MSTRLPAPAFEGKICTMDELAERAAALAHPVVMTNGVFDILHRG
                     HVTYLAQARSLGGSLVVAINSDASVKMLGKGDDRPINTEADRAAVLASLESVSLVVVF
                     QDKVPLKAVELAHPDIYVKGGDYRIEDLPEAKLVSTWGARTVTVQFEHERSTTALLKK
                     VRGA"
     gene            complement(5434..6012)
                     /gene="gmhB"
                     /locus_tag="ACZPB6_00320"
     CDS             complement(5434..6012)
                     /gene="gmhB"
                     /locus_tag="ACZPB6_00320"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR1182956.1"
                     /translation="MMRKAAFLDRDGVINIDHAYVHKIEEFDWVPGVLEAARALSEAG
                     YLLVVVTNQSGIGRGYYDEAAFTRLTDWMKARFAEAGAPVAGVYFCPHHPEKANPPYR
                     MDCDCRKPRPGMLLKAAEDLGIDLSTSIMFGDKPGDMTAGRAAGCVERIQLGTDGLET
                     PVPSADATGAFRSLADAVASPWFAQLKQRSLP"
     gene            complement(6009..7013)
                     /gene="rfaD"
                     /locus_tag="ACZPB6_00325"
     CDS             complement(6009..7013)
                     /gene="rfaD"
                     /locus_tag="ACZPB6_00325"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HIR1182957.1"
                     /translation="MSILVTGAAGFIGCNNVLALNERGITDIIAVDNLERGEKYQNLA
                     KCSISDYFDKRDFIARVRDGSAPKPEAIFHQGACSDTMEHDGKYMMENNYRYTLELYR
                     WAQELGIPFIYASSAATYGAHQEFIEDVRYEGPLNVYGYSKYLFDQVLRRELKSLRAP
                     VIGLRYFNVYGPNEQHKGRMASVAFHQYFQYRRTGKVKLFEGCLGYGNGEQSRDFVYV
                     GDVCKVLMHFLDKPVSGVYNCGTGRAQPFNDVALSVINALREHDGRQQLTLEEAVATG
                     ELEYIAFPEALKGKYQAFTQANLENLRKAGCDVEFRTVQEGTREYMQNLLAAFPNVEE
                     "
     gene            complement(7026..7946)
                     /gene="rfaE1"
                     /locus_tag="ACZPB6_00330"
     CDS             complement(7026..7946)
                     /gene="rfaE1"
                     /locus_tag="ACZPB6_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HIR1182958.1"
                     /translation="MDKQTLSKARVLVTGDAMLDRYWFGDAERISPEAPVPVVRISRT
                     EERLGGAANVAVNIASVGGRSSLLCVVGNDESGRTLERFAREQGITPHFQFDESLDTT
                     VKLRVVARQQQMLRCDFESHPAEEVLNKHLDKFRALLAEHDALILSDYGKGGLSHIVS
                     MIAMARDAGIPVLIDPKGDDYSRYRGATMLTPNRSELRQVVGSWRTEEELTAKAQGLR
                     AELGLKYLLLTRSEEGMTLYFEGGQITVPTQAREVYDVSGAGDTVIAILATMLATGMT
                     VEEAVRTANRAAGVVVGKLGTATVSYEELF"
     gene            8158..9540
                     /locus_tag="ACZPB6_00335"
     CDS             8158..9540
                     /locus_tag="ACZPB6_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR1182959.1"
                     /translation="MAPDASIFKAYDIRGIVDKTLTEDAVRQIGRVLGTMAHEAGRTS
                     FCVGRDGRLSGERLAGALMEGITSAGVDVIDIGMVPTPVLYFATVHFGNGTGVAVTGS
                     HNPPEYNGLKMMIGGITLFADQIQGILARIRNEDWRTAEVPGTIRREDVVPAYLERVL
                     SDVRLERPMRIAIDAGNGVAGPVAARLFKSLGCEVTELFTDVDGTFPNHHPDPSKPAN
                     LEDLKKAVLEDGIELGLAFDGDGDRLGVVTKSGEIIYPDRQMMLFAKDILAHHPGAQI
                     VYDVKCTRRLVPWIEACGGVPTISCTGHSLVKAKLRETGAPFAGEMSGHLFFNDGRWP
                     GFDDGVYAGARILEILSREADPSAALEALPSAVNTPELQIRMAEGENKAFIARLQKEA
                     RFDDALDVITIDGVRVEWNDGFALARSSNTTPVVVLRLEGDTPEALERIKKTFAEVLL
                     KVEPSLELPF"
     gene            9565..11019
                     /locus_tag="ACZPB6_00340"
     CDS             9565..11019
                     /locus_tag="ACZPB6_00340"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR1182960.1"
                     /translation="MTDSNNWTLLPFLRDSAGVDTMVTACGIRLDISRQRLTGEDLKR
                     LVDHCRTRGVEEAHRRMMAGEIVNPSEGRAALHTSLRSFSAGAPKFAEVDRERRRMLD
                     FARQVREGIRLGCRGDRITDVINVGIGGSDMGPHAVYHALRPSNPKIRVHFLSTVDGV
                     LLERILDVCNPLTTLVVVSSKSFGTRETLVNAAAVDQWLLNHGIVGTDRAHHMVVVSA
                     NPDAAAQMCLPEENFFRMWDWVGGRFSVWGAVGLPLALSLGPEVFLEFLQGAAEMDRH
                     SLTAPLEKNLPATLAMIAFWNANRLEISTHCVLPYDERLRVIVPWLQQLEMESLGKNH
                     TPDGRRIPGRTGQAVWGANGNEAQHSFYQWLREGTGRASIDLLWSEMPGHRYAEHYRV
                     LLANARAQAEALIMRDPDNPCFNAVSAIVMDAVTPRRLGALMAMYEHKTTMLGTLFGI
                     NPFDQPGVELGKRLSKRAERGEDPMAAVAEEVRF"
     gene            11020..11982
                     /gene="waaC"
                     /locus_tag="ACZPB6_00345"
     CDS             11020..11982
                     /gene="waaC"
                     /locus_tag="ACZPB6_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HIR1182961.1"
                     /translation="MKILVIKSSSMGDIIHALPVAHDIRQALPEAELHWVAEESFRDI
                     PTLAPAVSKVHVTAFRRWRKAILSGPVRAEMADVKNALRAEHYDVVLDIQGLMRSAMV
                     ARWAGVPATGYSWGTAREPIASLAYARKLDLPASLGAVRRYRMAAAEALGYEIDPERP
                     VFGLQANAEPSVKPAGPYAALAVNTSRDEKLWPEEYWTHLGRKFAGAGLRSVLFWGSD
                     VERERVERLVRTIPGAEVAPRTTLATTAATLARAECVVGVDTGLAHLGAALGRPSVGI
                     FVSTPTETLKLVGDGPVRSLGGIGQCPDVDEVWKALVAVQGERL"
     gene            11979..13292
                     /locus_tag="ACZPB6_00350"
     CDS             11979..13292
                     /locus_tag="ACZPB6_00350"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HIR1182962.1"
                     /translation="MRITPSMYRAVSVVALPLASLYLMWRSRRQPAYRQFWDERFAWS
                     SFPLKTGRPRVWIHAVSVGETNAAKPLLEAVLERWPECDVLLTHMTPTGREAGAKLVA
                     LAPDRIHQCYLPYDAPYAVEKFFRQTQPTIGIIMETEVWPNLMSEAKRCGVPMVLANA
                     RESEKSRAQAEKFLDVMGPAFGSFTAILAQSEEDKERLESLGGRNVTVCGSVKFDIRP
                     NASQEAAARAWKASLSRPVALIASTRQGEEVLFAKAMATRPELLRRAQFWLVPRHPQR
                     FDEVFRTLTGAGLKVCRRSQIAEPADVPADTDVVLGDSMGEMSFYCALADMTAMGGSF
                     QNHGSQNVIEPALAGSPIVVGPSIFNFQKVIRDGLSAGAMVQVETPEEALDRFEHWLD
                     VPETRVSASEAALGFSRQYAGATQRMMTILEVLWQKAQKTASLTS"
     gene            13253..14092
                     /gene="thiD"
                     /locus_tag="ACZPB6_00355"
     CDS             13253..14092
                     /gene="thiD"
                     /locus_tag="ACZPB6_00355"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR1182963.1"
                     /translation="MAEGTENSIPNVVSIAGVDPSGGAGVLADVKAMSACGAYACGVI
                     AALTAQNTQTVTGVMPVPATFLKEQIDTLFDDVRIDAVKIGMLGTVEQIETVADALVR
                     RKPRFVVLDPVMVAKGGSRLLAADAVDALKERLLPLATIITPNLPEAMCLLGREEDVT
                     EEEFPKVARELWKLLGRRNAWVYLKGGHLEGESAYDLLFNNVVSVPFTAPRVPTKNTH
                     GTGCTLSSAIAAFLPQSVDVPEACRRAKEYLTGAVRHAGELDVGHGHGPTNHFWRLWK
                     ERV"
     gene            14152..14228
                     /locus_tag="ACZPB6_00360"
     tRNA            14152..14228
                     /locus_tag="ACZPB6_00360"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14186..14188,aa:Met,seq:cat)
     gene            14332..15660
                     /gene="miaB"
                     /locus_tag="ACZPB6_00365"
     CDS             14332..15660
                     /gene="miaB"
                     /locus_tag="ACZPB6_00365"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR1182964.1"
                     /translation="MQAKKLYLRSFGCQMNDYDSGRIADLLNAEMGYERTDVLEEADL
                     VVVNTCSIREKAQEKTFSDLGRIREVKKTRPGMLIAVGGCVASQEGENVIRRAPCVDV
                     VFGPQTLHRLPDMLRARVETGRPQVDVSFPEIEKFDHLPAPKAHGAAAFVSIMEGCSK
                     YCSYCVVPYTRGEEISRPMVDVLMEVAQLADQGVREVTLLGQNVNAYRGKMPDGSIAD
                     FALLLDYVSEIDGIERIRYTTSHPREFSQRIIDAYGRLPKLVNHVHLPVQAGSDRVLA
                     AMKRGYTAAEYVEITRKLRELRPDISIATDFIVGFPGETAEDFEDTMKLVEKVGFDAS
                     FSFIYSPRPGTPAARLPDDTPYDVKLARLQRLQATIDANATRISESMLGTEQRVLVLG
                     PARKGEGFLMARTDNNRIVNFEGPLSLVDTMVRVKVTDVYPHSLGGQLLD"
     gene            15671..16702
                     /locus_tag="ACZPB6_00370"
     CDS             15671..16702
                     /locus_tag="ACZPB6_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR1182965.1"
                     /translation="MASEKTVQPETLRYSADAGNAELSALCGPLDENLRQIETILNVR
                     VSRRGDVFRVTGELEAARRAVTALAVLMTRVKSTGEELSVDDIQWHFVGHDDEYRSGA
                     ASDPEGAVDDVDLKVRRRDLQGRTPNQRLYLKAIMGHDISFGVGPAGTGKTFLAVAAA
                     VDAFERGTVERIVLTRPAVEAGERLGFLPGDLTQKVDPYLRPLYDALFDLMGFEKSQR
                     LMERQSIEVAPLAYMRGRTLNNAFVILDEAQNTTPEQMKMFLTRIGFGSKAVITGDVT
                     QIDLPKGVPSGLKHAIRILDGVRGIEITRFTSKDVVRHPLVARIVDAYEEAAKQDELA
                     AKARKEVKE"
     gene            16699..17160
                     /gene="ybeY"
                     /locus_tag="ACZPB6_00375"
     CDS             16699..17160
                     /gene="ybeY"
                     /locus_tag="ACZPB6_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR1182966.1"
                     /translation="MSEPAVKFSFQNAAAFPQLPSRSVMIRWMTRACELPTMMTVRFV
                     DQEEGYELNHSYRHRDYATNVLTFNYTTEPEVTADVAICVPVVEREAAEQNKEFRAHL
                     AHLLIHAVLHAHGYDHVTQEEAEVMEARETELMLSLKFPNPYSDRIGMVHD"
     gene            17230..18039
                     /locus_tag="ACZPB6_00380"
     CDS             17230..18039
                     /locus_tag="ACZPB6_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR1182967.1"
                     /translation="MGRTFLDRLTSVFHEEELNDRADVLEVLGEARRRNVIDDEVFSM
                     MEGAMAVSEIRAGDLMVPRSQVQAVDFSEPREVWLPALIASGHSRLPAVEDDLDNVLG
                     ILHAKDLLQVLMNPAVDPKTLLRPARFIPETQPINVLLRDFKETRSHLALVIDEFGSI
                     SGLITIEDVLEQIVGEISDEFDHDEEGSNIVADGEGRWRVKAITPIEQFNDYFKSNLE
                     DQYCETIGGLVTDRFEHVPHTGETIEENGFRFRILGGDDRQARLLSVERIK"
     gene            18041..19594
                     /gene="lnt"
                     /locus_tag="ACZPB6_00385"
     CDS             18041..19594
                     /gene="lnt"
                     /locus_tag="ACZPB6_00385"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR1182968.1"
                     /translation="MSEASHWWERRPEAAGALGALGMGLLFGTGFDPLGWQWMSLIAL
                     VGFFLVFAARPTPARAALVGFLFGLGWFVPGLSWTMNSIAVYGRLPWAVAALALLVLG
                     AVLSLFPAFACWIAKKIGGGRLATELPALAGVWTLAEWLRGDVLANFGWLMPGYAVID
                     TPLAGWAPLGGIYAVTLAQLLFAALVAVALSCRLAWLAVPVIGAALIAGTGQVSRQHA
                     WSEPSSTVDVRIIQPALPVVDAYTRANPAQRIEALFPAAFKPWPASDRPRLLLTPEGI
                     VNVPVSRLGREAAGRLVLLQEKTEARVLFNGFRKDGPRFFNTSFVLDEGQIAYRLDKR
                     ELVPFGEYVPAGFHWFVEMIGIPMSDLMRGDAVQPLLSLGGADAGILICYENLYGSVV
                     RTFWQSRSPDFLIVTSNLGWFGRSVLGQHLTMSRMRAIESARPLVSVSNTGMSALVNS
                     RGEIAAMLRTDGPDAATMPLLGATGSPTPYVRLGSWPALLASLVLALCAVLVRLGTAR
                     IRAGKVKGL"
     gene            19687..20595
                     /gene="glyQ"
                     /locus_tag="ACZPB6_00390"
     CDS             19687..20595
                     /gene="glyQ"
                     /locus_tag="ACZPB6_00390"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR1182969.1"
                     /translation="MITFQEVILRLQEYWNRQGCALLQPIDLEVGAGTSHTATFLRAL
                     GPEPWRAAYVQPSRRPKDARYGENPNRLHQHHQFQVVLKPAPTNIVDLYLGSLRALGI
                     DTEVNDIRFVEDNWENPTLGAWGLGWEIWMNGMEVTQFTYFQQVGGLECKPITGEITY
                     GLERLTMYLQNCDNVFDLVFTTWTDPDGTERKLTYGDVFHQNEVEQSVYNFEASDCEK
                     LLKQFDYFESEAKRLMDLNLALPAYEMVLKAGHTFNLLDAHGAISVTERAAYIARIRN
                     ISRSVAQSYYDSRERLGFPMLAKSAE"
     gene            20619..22709
                     /gene="glyS"
                     /locus_tag="ACZPB6_00395"
     CDS             20619..22709
                     /gene="glyS"
                     /locus_tag="ACZPB6_00395"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR1182970.1"
                     /translation="MTNLLVELQTEELPPKALEKLSEAFAAGVEQHLRNRGFLAEGSV
                     TTAYGSPRRLAVHISNVLEKSPDEAFTQKLVPVRVGLAADGSATPALVKKMNALGITC
                     DVAELKRVNDGKNEQLVFEGVRPGIVLAEGLQTALDAAVKNLPIPKVMTYQLADGETT
                     VAFVRPVKHLTALYGEQIVPVKLFGLNAGRITRGHRFHTTEPVTLSCADAYDAELKAA
                     KVMPCYAERRELLRKLLTERAAQIGGTVIMPEDLLNEVTALTEWPIIYESSFEEEFLA
                     VPEECLILTMQLNQKYFALRDAEGRLMNRFLLVSQLEAKDGGAAISSGNARVVRARLA
                     DAKFFYDQDRQEKLETRVDGLKHVVYHNKLGSQAERMLRVKTMAGLFADLIGADRAKA
                     ERAAMLAKADLRTLMVGEFPELQGIMGEYYAKYDGEAEEVALAIREHYQPRYAGDALP
                     STPVSLATALADKMETLIGLFGIGQMPTGEKDPFALRRHALGVLRMLIEKALPVSLNT
                     LIDAAWEAEKAVPGVTDHREELRQFFQDRLRVMLRDAGYTTLEVDAVLALNPEKLDEL
                     PKRLAAVRAFMALPEAEALTAANKRIGNILKKIEGEVPTVVNEALLAEDAEKQLFAAI
                     NDVEPKALQHFAAGEYEQMLATLAVLRGPVDSFFDNVMVNAEDPALRATRQALLKRLY
                     DVMNKVAEIARLAK"
     gene            22821..23543
                     /locus_tag="ACZPB6_00400"
     CDS             22821..23543
                     /locus_tag="ACZPB6_00400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1182971.1"
                     /translation="MNLLRGWLFYIVLSFSILVLAVCVLCTTPFASVKWRYEAICRPW
                     AKFVLALLRVICGIRLEMKGMENLPPADTQAVVLMKHQSAWDPFWLGAYLPAPACFIY
                     KRSINWIPVLGWVVCAMDMLAIDRSKGRSAFEAFMKKGPVKLREGFWISLFPEGTRVP
                     PGERVRYKTGGARFACQSGTPIVPVCHNAGHCWPKNSIAKQPGTITVSVGPVIETKGR
                     DPHEVTAEVEKWIEDELERIDA"
     gene            23590..24348
                     /locus_tag="ACZPB6_00405"
     CDS             23590..24348
                     /locus_tag="ACZPB6_00405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR1182972.1"
                     /translation="MTAEDGERIEYLFQPSRRHRTRVILSYDGRQDKPVVTVPYRYAG
                     PQMLSDEFVLQRCDEIILQKREYVRWMRAQTHAVLDAAYCIAGGSGYILYRGRKTAVA
                     VDATAKTSYVDVTGDDAVIRLALPQTCSRKEVDRALLALFKVQAKTVLGDLMEAYRGS
                     ARHWPASWSLSSARMRWGCCTTRNTIRLCWRLIFLDDAVASYVIAHELAHLEHFNHSK
                     AFWKEVERLCPDWRVQLAKLNAAKVLPELEEVGQ"
     gene            complement(24352..25167)
                     /gene="rsmA"
                     /locus_tag="ACZPB6_00410"
     CDS             complement(24352..25167)
                     /gene="rsmA"
                     /locus_tag="ACZPB6_00410"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR1182973.1"
                     /translation="MKQEWLEGHKARKRFGQNFLHDRHWIERIVRGIDPKPGDALIEI
                     GPGQAALTREVIACAGHEAAVEIDRDLAQFLREQFSPEELTLIEADALKLDWTTVLEG
                     RRLRIIGNLPYNISSPLLFALMSAADRVIDQHFMLQREVVDRMVAEPGSKTYGRLSVM
                     LQYRYVMHKLFDVPPGAFVPPPKVTSSIVRMVPRPVESLPEVDMEIFSQVVALAFQQR
                     RKTLRNALSTLMDDAAIKRAGVNPAARAETLDVAAFVRLAREAANLPHHADAV"
     gene            complement(25148..26119)
                     /gene="pdxA"
                     /locus_tag="ACZPB6_00415"
     CDS             complement(25148..26119)
                     /gene="pdxA"
                     /locus_tag="ACZPB6_00415"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR1182974.1"
                     /translation="MTELRSVAVTTGEPAGIGPEVSLKAAAAVSDPILLIGDRTLLEA
                     EARRLGIAWPLPAHVSIRHVQLRAPAEPGRLDTRNSAYVLDILRAAHEGAAAGEWSAV
                     VTAPVQKSIIIESGVPFTGHTEFFQDLCGVRRVVMMLTSNARTDAMKVALATTHLPIA
                     RLSEAITGELLDEVLDTLNTALARDYGLAEPRILVTGLNPHAGENGHMGHEEINTITP
                     ALERARSRGIHADGPVPADTAFVPGHWDRYDAVLCMYHDQGLPVLKHVGFAEGVNVTL
                     GLPYIRTSVDHGTALDIAGQGKADDTSMISALRLAHFLSKNRETRMA"
     gene            complement(26128..27486)
                     /locus_tag="ACZPB6_00420"
     CDS             complement(26128..27486)
                     /locus_tag="ACZPB6_00420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1182975.1"
                     /translation="MNKLKTIAVLTMSMAALSTAFAAEQAVTESAQAAPVVQPQAGNV
                     ELDRIVAVVNNDVITDRELQERTHVVAINLRRQNIQLPPMEQLRAQVLERLIFERAIK
                     QRARETGIRIDDQMVNASIEQIARQNNLSVDQLRQRLMADGVSFSSFREEIRDEITTQ
                     RLREREVDAKIQIPESEIDAYLAEQAGFTTGNTTEFHIQHILIPIDHPDNTEKLKKHA
                     EEIAERARAGEDFAMLAATYSRASDALQGGDIGWRNLQSLPNVMADVLKESPRKGVYI
                     IKGKDSWDVMRVSDTRDGVQFKLAGGPVEQTHARHILMLVSDITPEGEVIHRLNDIKR
                     RIDAGESDFATMARLHSVDNSATRGGDLGWLQPGDTVPEFEKAMNSLKPNEVSEPIKS
                     QFGYHLIQVIERRMEKNGNPQRMRIAARQQLREKKLGEAVYNWQRELRDRAYIEIRDS
                     HY"
     gene            complement(27479..29794)
                     /locus_tag="ACZPB6_00425"
     CDS             complement(27479..29794)
                     /locus_tag="ACZPB6_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR1182976.1"
                     /translation="MSFPRPRQTAISVSIALAAMAYTSIAPAAQTGSVVSLESVTTLD
                     PSRATPDSDAASFVSADRIEGNPEDQLHLYGNAEIRRAGTVLKGDRITYTQETDEVEA
                     VGNARISRQGASFSGPSMRFKISPRTGDMSDAEYEYAPRNIRGCAKNIRFISGESTVF
                     DDAKVTTCKRDDEAWFIKMNELEIDEYDQTATGTNASLHFMGVPVFGSPWFSFPVSRE
                     RRSGFLVPTFGMSSTRGLDLAVPYYFNIAPNYDLTLTPRLMTKRGVMLDTEARFLYNN
                     FETTVDYALMPDDKEMDDSRSSLHVDMSWRRDKLSAFVDYNRVSDDDFISDFSSNVRE
                     SSETVLPQEYALRYDETYWNTSINVKKNQALTFDHIDYYKPYERVPQITFNGYNGDFY
                     GFEVGTTIEATRFEHPTFTTGDRFVFEQTVSYPMRDAGWFVVPKGTFLGTWYQLENLD
                     RDGGRFTNKDPHRTTPMFSLDAGLVFDRDAVWFNREAYQTLEPRVYYAYVPYRNQDDL
                     PVFDTTIADLNFATLFSENIFSGYDRVSEANQLTSVLTTRYIDKTSGLELFKASVGQR
                     NYFSDQRVNFYTPGDTQMRESPLRTDVRSDLLASVGARLTRSITSDVSGQYSSAMSRW
                     VKAKAGVRWQPRPMSLVGLYYRYNYSSERDYTDYQNDYIKQIDLAVQWPITNRLFGLF
                     RYNYSLLQDKPIEMIGGVEYLHDCWTLRFAAQRYTTTSNNDETNFFLQLELNGLGSIG
                     TSPLSELQRNIKGYQTTTRVPDKIGQYDYYE"
     gene            29951..30970
                     /locus_tag="ACZPB6_00430"
     CDS             29951..30970
                     /locus_tag="ACZPB6_00430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR1182977.1"
                     /translation="MTNSAAASAFWQWLQSISPKYQLDLTTVSRAGADAGFRSYHRIA
                     AADGRSFIAMNAEGETPESFASFIKVDGLLRNEAGLNAPEIFEADTDHRFMLLSDLGR
                     ETYLDVLNEDNARELMDRATTALVAWQKISRPNVLPAYSREVLQRELELFPTWYVGRH
                     RGVEWTEEQQKWWRMTTEAILDTNCREALVFVHRDFMPRNLMVSDPMPGILDFQDALY
                     GPLSYDIASLLRDAFISWDESFVLDTTIRYWEKARKAGIPVPADFGNFYRDVEFMGVQ
                     RHLKVLGIFARLNYRDGKPKYLEDTPRFIQYVRKTARRYIQLSPLTHLIDDLEGSETS
                     FGYTF"
     gene            30976..31683
                     /locus_tag="ACZPB6_00435"
     CDS             30976..31683
                     /locus_tag="ACZPB6_00435"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1182978.1"
                     /translation="MRALILAAGEGRRMQPVTLTTPKPLVEVAGTPMAVRQILALRKA
                     GVEEIVLNVAHLGDRIVETLGDGSAWGVRLRYSVEGRTAGEALETLGGIAKALPMLDD
                     GDGAFIVVAGDIVTDFDYSRLVRAGRALAGSDAVAHLVLAPNPSYHADGDMTLDELGL
                     VRREPRTHTFSSLGVYGTSLFEGVEPVRAKLFPWLWQFCEAGRVTGEVYEGMWFNVGD
                     VRELALAEARIRNQMEK"
     gene            31780..32766
                     /gene="dusB"
                     /locus_tag="ACZPB6_00440"
     CDS             31780..32766
                     /gene="dusB"
                     /locus_tag="ACZPB6_00440"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR1182979.1"
                     /translation="MKLGPYAVDVPVMLAPMAGMSDLPYREICRAHGAGYAVGEMTTC
                     QPQFRTSRKSATRWACDAELGLRVVQLLGADPAMMADAARYAEDSGAQVVDINMGCPA
                     KKVLQTACGSALMKDEALVARILEAVCAAVSVPVTLKIRTGWDREHRNALAIAGIAEQ
                     TGVAMLTIHGRTREDGFRGEAEYETIRTVKAAVSIPVIANGDIDSGEKARRVMALTGA
                     DGVMIGRASYGNPWIFSEVGAALGHLPERKAPGLEERHAVICDHMRRHFDYYGGARGA
                     VTFRKHLSHYLKGLPGSEALLGELFATSDADVLGAAVSRYFERLQDDEQGLG"
     gene            32759..33145
                     /locus_tag="ACZPB6_00445"
     CDS             32759..33145
                     /locus_tag="ACZPB6_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014950.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1182980.1"
                     /translation="MDDKAIVRRKPEAPQIRLIRQAKQRVKLGLDFEGVTLESLAKDM
                     PELKAGRIEEAVIVQLNQYFQQLGGSQPHPLYSLVMETAERPLLIYVMKLCRNNQIEA
                     AKILGINRNTLRSKLRSHKLLPGESS"
     gene            33157..34710
                     /gene="purH"
                     /locus_tag="ACZPB6_00450"
     CDS             33157..34710
                     /gene="purH"
                     /locus_tag="ACZPB6_00450"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR1182981.1"
                     /translation="MKKQALISVSDKTGVVDFARELVALGYHILSTGGTAKLLTKEGV
                     PCQEVADYTGFPEMLDGRVKTLNPKIHAGILARRPVAEHMAALEAHGIDPIDIICVNL
                     YPFEQTIAKPDCTFEDAIENIDIGGPTMIRAAAKNHESVAVIVDPTDYASVVAEIKAE
                     GGVTAATRLALAKKVFAHTARYDGMITNYLTSLDENNQRTRFPQVLTRQWEKVQDMRY
                     GENPHQAAAFYRESSVAPGLLAGYEQLQGKELSYNNIADSDAAWECVRSFEAPACVII
                     KHANPCGVAVGISSAEAYSKAFQTDSKSAFGGIIAFNREVTGECAELVSRQFAECIIA
                     PSFSKEAVEIFAAKKNLRLLTVALGKAHNDFDFKRVGGGLLVQTPDNQICAEGALRCV
                     TKRQPTSSQIADLMFAWNVARFVKSNTIVYARGGMTLGVGAGQMSRVDSARIAMLKAE
                     EAGLSLEGSVAASDAFFPFRDGLDVVVDAGCTAVIQPGGSIRDDEVIAAADERGIAMV
                     FTGERHFRH"
     gene            34850..36145
                     /gene="tolB"
                     /locus_tag="ACZPB6_00455"
     CDS             34850..36145
                     /gene="tolB"
                     /locus_tag="ACZPB6_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR1182982.1"
                     /translation="MTNFNPSRRRVLCAGVLAGAFTACPALAELQVSITGVGANQLPI
                     AVKVFEGSGATGFDAAAVIGADLERSGAFRLIPTGAEGEPENLEKPASLEAFGKLGAN
                     ALAVGRVSCLPDGRWELTYYLHNTVTGALTDSAVFTTSGNDVRMCAHRIADRIYTNLT
                     GEGPIFASKLVYVAQLARNRYELVISDSDGGNRQAALQSHEPIISPVWSPDGKKISYV
                     SFEDRKPIIYVQELATGARHAICAFRGNNSAPAFSADGGTLAVALSRDGLTQIYLMNA
                     NGTGLRRFTKSYGIDTEPCFSADGQWVYFTSDRGGAPQIYRQPLAGGPAERVTFGSSY
                     AISPTVSRDGRYLSYISRIDGRFRTAVMDLTTGQNTLVTTTDRDESPSFAPNGRFLVY
                     ATEVNGRGVLGTASVDGRLSTRLTGLGDIREPCWGPILP"
     gene            36200..36727
                     /gene="pal"
                     /locus_tag="ACZPB6_00460"
     CDS             36200..36727
                     /gene="pal"
                     /locus_tag="ACZPB6_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIR1182983.1"
                     /translation="MSFSWKILAASAAVAALLSGCSSVSLDESAKDGTLAGQTAGSQT
                     QAVNPFADPNNPLSKRSVYFSFDSYEVEAQYMPAVEAHARWLAAHPNVNIIIQGNTDE
                     RGGREYNLALGQKRSEAVKQRLQLLGIPAERIEAVSFGREKPVAYGHSEEAWAQNRRA
                     DIVYPTEVTSGKAAQ"
     gene            36845..37570
                     /gene="ybgF"
                     /locus_tag="ACZPB6_00465"
     CDS             36845..37570
                     /gene="ybgF"
                     /locus_tag="ACZPB6_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIR1182984.1"
                     /translation="MNLNKLMVQACTAAILSGSAAGAMAFADDDARRAILDLRAQVAA
                     LEEQLKNAQISFATRLDNLQNQNRLLTGQVEELTNAIRQEKRSTRELYTSIDERLGKF
                     EPREVEVNGEKVMVQPQEQAAYDAAVELLKAGDYKKAAAAFSTFGAEWPKSAYAADAV
                     YWRGSCLFALEQYKSTINVQNSLIRSYPKHPRVADAMISVASSQAALGNVKAASATLA
                     KVIKQYPNSDAAKTAAQLQKTLK"
     gene            37843..39465
                     /gene="maeA"
                     /locus_tag="ACZPB6_00470"
     CDS             37843..39465
                     /gene="maeA"
                     /locus_tag="ACZPB6_00470"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HIR1182985.1"
                     /translation="MTESKQICGADLLRNPQLNRADAFTQEERDARGLRGLLPPAVST
                     MELQVKRTLALLDRCPTALDKFLMLDSLHATDEDLYFKILIDYIDDYMPVVYTPTVGE
                     VCQKFSHIYRYPRGAFISINDKGRVREIIENCPNEEVDVIVVTDGQRILGLGDLGING
                     MGIPCGKLSLYTACAGIAPEKTLPVTIDVGTNNPEYLNDPMYLGLRQERVTGEAYDEL
                     IEEFVTEARRRWPNVLIQFEDFGNNHAFDLLERYKDKVLCFNDDIQGTAAVAVTGVYS
                     ALRVTKQKLADQRFLFLGAGEAATGIAELLVDAMVAEGVEREDALKHCFLFDSKGLVT
                     CNRGDKLAHNKVPFAHDVENCKTFIEAVRALRPTGIIGVAAQPGAFNAEVLGAMAEIN
                     ERPIVFALSNPTSRAECDAATAYRETNGRALFASGSPFAPVDFNGKTFVPRQGNNSYV
                     FPGLGLGAVFARAKTMPVGMFLAASRELAELVSEADLEQGSLYPPLSTVREVSVKIGA
                     AVAQYAYDNGLAGNERPADLEAAVREFMYKPE"
BASE COUNT         6969 a        12841 c        12692 g         7166 t
ORIGIN      
        1 gaggcccatc ccagacaggc gggcgtctac ggcgtcacga agcacgaggg ggagaggctc
       61 cttgaggaaa gcggcgtttc gggagcgatc gtgcgcgcgg gttggcttca cagcggcgag
      121 cgcgactttg tgaacgccat tctgtcggcg gcggccgagg gcagggctct ctacgtgacc
      181 gcctctcaga tcggtacgcc tacttcgacg gaggcgctcg cccgatggac ctgggacgcc
      241 ctgcccgaat tgacgcctgt cgatcgactg acgacggtcc actacgtgga ggaaggcggc
      301 tggatcagca gggccgatat ggccgttcat gcactggagg cggccgagga ccgggccatc
      361 aggctcggtg aattcaagct ggccattcgc ttccgaaagg ccagggaggg catgcggaga
      421 acggatgccg acgacggctt caggccgctc aactgccggc tctcgagtct caaaaaggac
      481 gagtttccgc ggacggaacg gtggatggag ggtgtggata agtctgtgga tatcgttgtt
      541 gaggccaaaa aatccctgtg gataactcaa aaatgagagt ctgaatctcc ttgaatttca
      601 ataagatgaa atggaaacgg ccgccaaggg ggcggccgtg gataactttg gctctcctgt
      661 aggggagcgg tgcgtcagcg cttgttggac tcgaagttcc aggcgtccct gcacatgtcg
      721 tcgatcgtgc gggaggcctt ccatccgagg agcctttcgg cgcgggcggg gtcgcaccag
      781 ttggcggcga tgtcgccctc gcggcggtcg acgagttcgt agggaacgga acggtcgctg
      841 gccttttcaa aggccttgac gacttcgagg acgctgtagc cgcggccgca gccgaggttg
      901 atggcgatcc aacccgtgtg gttcatgatg tagggcacgg ccttggcgtg gccgtcggcg
      961 aggtccatca cgtggatgaa gtcgcgcacg cccgtgccgt cgggcgtcgg gtagtcgtgt
     1021 tcgaagacgc gcaggcgggg cagcgtgccg tgagccacgc gggcgacgta gggcagcagg
     1081 ttgttcggaa tgccgttggg atcctcgccg atgaggcccg agggatgggc gccgatcggg
     1141 ttgaagtagc gcaggcagac ggcgctgaat tcgggatagg cgcggcagac gtccgcgaga
     1201 atctgctcga tgtgcagctt cgagcggccg tacgggttcg tcggttcgcc ggtcgggcaa
     1261 tcctcggtga ggggaagcgt ctcgggcgtg ccgtagaccg tcgacgagga gctgaagatg
     1321 atgcggttga cgcccttcct gcgcatggcc gcgagcagca cgagcgtgcc gttgacgttg
     1381 ttgtcgtagt actcgacggg cttgctgacg gattcgccca cggccttgag gcctgcgaag
     1441 tgaatgacgg cgtcgacgcc gtcgagcgcc ttctccatgg cggcggcgtc gcggatgtcg
     1501 ccttcgatga agacggggcg acggcccgtg atcgtctcga ttcggtcgag aacggagcgg
     1561 tcggcgttgg agagattgtc gaagagcacg acgtcatggc cggcctcgat gagcacgacg
     1621 gccgtatggg agccgatgaa gcccatgccg ccggtgagca gaatgttcat gtctgggtcc
     1681 tgcgaggtga agacgggagg cgcgggagcg ccgtcccctg aggtggtggc attgtaatgc
     1741 tgcggcgtga ttgcaacaac agtcttcccg aatcggcgtg cggcccggcc gtataatcac
     1801 agtttttttg caggatcgag catgcaggaa ggaaccgtac tctgctttga cttcggactg
     1861 gccagaaccg gcgtcgcctc gggcaatacg ctcacgcgca gcgcgcagcc cgtcacgatc
     1921 attcatgcca gaacgaacga cgagcgctgg aaggccgtcg aggccctcgt gaaggaatgg
     1981 gagcccgtgc ttctggtggt cggcgtaccc cgtcacgggg acggcacgga ctgcgacctc
     2041 acgccccgtt gcgagcgctt cgcccgccag ctcggcggcc gcttccgcct gcccgtgcgc
     2101 accgtcgacg agcgcttctc gtccgtgatg gtcgaacacg gcgcggacaa gatcgacgac
     2161 gaggccgccg ccgtcatcct gcaacagtac ttcgacgagc aggacgccgt cgcctgagga
     2221 agcctttcat gcgctatgtc atcggatgcg gccgcatggc cgccgtgggg atcatgatcc
     2281 ttgtgatcct cttcatcctt ctcgtgcttt tcccgctggt cggccgcaag ggccgcggcc
     2341 gctgcgtcag gcgcgtctgc cgcgtgctca tgcgcgtgct cggcgtgcgc atgaccgtca
     2401 ggggcgcggt gcccgtcgcg cagagcgccg aatgcggcgt gaacggagag ggaccgggct
     2461 acatggtctg tgcaaaccac gtttccttca tcgacatctt catcctcgat gcggtccttc
     2521 cgtgccgctt cgtcgccaag aaggagatcg cctcctggcc cgtcttcggc ttcatcgcca
     2581 ggggaacagg cacgctcttc atcgaccgca gccgcaagcg cgccgtgctt gagatcgccg
     2641 acgccatggc gggcgccatc aacgagggca ccaacgtgct cttctttccc gagggcacga
     2701 cgggcaacgg ccttgagctt ctgccttttc atccgaatct cttcgaggcc gccgtccgct
     2761 cgggcgcgca gattctgccc gtgacgctcc gatacacgct cgacggcaag ccgagcgggc
     2821 tcgtttccta tgcgggggac gtcgcgctct tcacggtcct caagcgaatc ctcttcacgc
     2881 cgggactcgg cgtcgaggtg acggtgctcg atcccgtctc cgtcgacggc aagacccgcc
     2941 aggcgctctg ccttgaggca tccgcgctca tgtcgggggc gctcggcgtc tccgacgtca
     3001 ccgctgaacg cgaagaggcc cgcaggcgcc gcctgcaggc catgtccgaa acgaaggccg
     3061 aagccgaaaa gaccgtctga gacggactta tttgtatttg aaggggtctg cccagcaggg
     3121 gcagtcctcc tgatagatcc gcctgtcggg gaagcgcacg gcggtgagct tcccgcccca
     3181 caggcatccc gtgtcgatgg ccacgatgtc gtcgcggcgg atgaggccca gcatggacca
     3241 gtggccgaag cagatgggcg tgcccgccat ggggcgcttc gagtattcga accacgggat
     3301 caggtgcttg ggtgcggagc cgatgtcctc cttctgcttg aagtcgagct cggccgtctt
     3361 tttgtccacg aaacgcatgc gcgtgaagca gttgagaatg gcctgcatgc gctcgccgcc
     3421 cttgagcccg tcgtcccagt cgctgttgcc gtacatctgc tggagcatgg ccttccagtc
     3481 gggccccgac agcacggcct cggcctcgcg tgcgagtccc ctggcctttt ccagcgtcca
     3541 gagcggatga acgcccgcat gcacgaagac ggcgtcctcg gtttcgatca tgatggactg
     3601 gtgcctgagc cactcgatga gttcgtccgc gtcgggcgcc tcgaagattt ctccgatcgt
     3661 gtccttcttg tgcaccttgc tcgcacccgc cgccacggcc agaaggtgca ggtcgtggtt
     3721 gccgagcagg catcgcgccc tgctgccgag gccagcgacg cgccgaaggg tcgctagcga
     3781 ttcgggcccc ctgttgacga gatcgccgag gaagatgagc ggggcgtcct ccgggagctg
     3841 ttcgagcaga ccgtccaagc tttccagaca gccctgcagg tcgccgatgg cgaagtacgg
     3901 gttcgtgacc ataagaatct ctaattcgtg ctagtcttta cgacaatttc agcgttgatt
     3961 gccccgactg agggacaacc ctgattttct caattgtact ttttattggt cacaagacaa
     4021 tgaagcctgt tcgcaaagtc gtgttccccg tcaacggtct cggcacccgc tttcttcccg
     4081 ccacgaaggc gatgcccaag gaaatgctcc ccgtggtcga catgcccctg atccagtacg
     4141 cagtggcgga agccgctgcg gcgggcatca ccgaaatgat cttcgtgacc ggccgcaaca
     4201 agcgcgccat cgaggaccac ttcgactcct atcccgaact cgagcgcgag cttgaggaaa
     4261 agggcaagga cgagcttctc gagctcgtgc gcggcatcgt tcccgccggc gtcaagtgca
     4321 tctacatccg ccagcccaaa gccctcggcc tcggccacgc cgtgctgtgc gccaagcccg
     4381 tcgtgggtga cgagcccttc ggcgtcatgc tcgccgacga cctcgtcgac gctccgagct
     4441 cctgcatcgg ccagctgacg gccgtgcgcg aagcccgcgg cggcggttcc gtgatcgccg
     4501 tccaggacgt tgctcccgaa gccacgaaca agtacggcat cgtgagcgtg gatgacacga
     4561 gcctgcagac ctcccagatg cgcggcatcg tcgagaagcc cgaccccaag gtcgcgccgt
     4621 cccgcatggc cgtgatcggc cgctacgtct tcgaacccga agtcttcgac tacctcgaga
     4681 aggtcacggt cggcgtcggc ggtgaaatcc agctcaccga cggcatcgcc tcgtcgctcg
     4741 acacggtgcc gacctacgcc caccgcttcg agggcacgcg ctttgactgc ggcagcaagc
     4801 agggcttcct tgacgcgacc attcacttcg cacgcaagcg cggcttcaag atcaactgat
     4861 cccgccttcc gcaaaaatga caaaacccgc acaggccgac agccgtgcgg gttttgttgt
     4921 ttcagagggc ttcgggaggc cgtcaggcgc cgcggacctt cttcagaagg gccgtggtgg
     4981 agcgttcgtg ctcgaactga accgtgacgg tcctggcgcc ccaggtggag acgagcttcg
     5041 cctcggggag gtcctcgatg cggtagtcgc cgcccttgac gtagatgtcg gggtgggcaa
     5101 gctcgacggc cttgagcggc accttgtcct ggaagacgac cacgagggag acggactcga
     5161 gcgaggcgag tacggcggcg cggtcggcct cggtgttgat cggacggtcg tcgcccttgc
     5221 cgagcatctt gacggaggcg tcgctgttga tggccacgac gagactgccg ccgaggctgc
     5281 gggcctgggc gaggtaggtg acgtggccgc ggtgcaggat gtcgaagacg ccgttggtca
     5341 tgacgaccgg atgcgcaagc gcggcggcgc gctcggcgag ctcgtccatc gtgcagatct
     5401 tgccttcaaa ggcaggggcg ggtagacggg tgctcacggg agactccttt gcttgagttg
     5461 tgcgaaccag ggggaggcga cggcgtcggc cagactcctg aaggccccgg tggcgtccgc
     5521 ggagggaacg ggggtctcaa ggccgtcggt gccgagctga atgcgctcaa cgcagcctgc
     5581 ggcgcggcct gccgtcatgt cgcccggctt gtcgccgaac atgatcgagg tcgaaaggtc
     5641 gatgccgagg tcctcggcag ccttgagaag catgccggga cggggcttcc tgcagtcgca
     5701 gtccatgcgg tagggcggat tcgccttttc gggatggtgt ggacagaagt agacgcctgc
     5761 gacgggggcg cccgcctcgg cgaagcgggc cttcatccag tcggtgaggc gggtgaaagc
     5821 ggcttcgtcg tagtatccgc gtccgatgcc cgactggttg gtgaccacga cgagcaggta
     5881 gcctgcttcc gagagggcgc gggcggcctc aaggacgccc ggaacccagt cgaactcctc
     5941 gatcttgtgg acgtaggcgt gatcgatgtt gatcacgccg tcacggtcga gaaatgcggc
     6001 ctttctcatc attcttccac gttcgggaag gcggcgagga ggttctgcat gtactcgcgg
     6061 gtgccctcct gaaccgtgcg gaactcgacg tcgcagccgg ccttgcgcag gttctcgagg
     6121 ttggcctgcg tgaaggcctg gtacttgccc ttgagggctt cggggaacgc gatgtactcg
     6181 agctcgcccg tcgcgacggc ctcttcgagc gtgagctgct gcctgccgtc gtgttcgcgc
     6241 agggcgttga tcacggacag ggcgacgtcg ttgaacggct gggctcggcc cgtgccgcag
     6301 ttgtagacgc cggagacggg cttgtcgagg aagtgcataa gcaccttgca gacgtcgccg
     6361 acatagacga agtcgcggga ctgctcgccg ttgccgtagc cgaggcagcc ttcaaagagc
     6421 ttgaccttgc ccgtgcggcg gtactggaag tactggtgga aggcgacgga ggccatgcgg
     6481 cccttgtgct gctcgttcgg gccgtagacg ttgaagtagc gcaggccgat cacgggagcg
     6541 cgaaggctct tgagttcgcg gcgaagcacc tggtcaaaga ggtacttgga gtagccgtag
     6601 acgttgagcg ggccttcata gcggacgtcc tcgatgaact cctggtgcgc gccgtacgtg
     6661 gcggcggagg aggcgtagat gaagggaatg ccgagttcct gggcccagcg gtagagctca
     6721 agcgtgtagc ggtagttgtt ctccatcatg tacttgccgt cgtgctccat cgtgtcggag
     6781 caggcaccct gatggaagat cgcctcgggc ttcggagcgg agccgtcacg cacgcgggcg
     6841 atgaagtcgc gcttgtcgaa gtagtcggag atgctgcact tggcaaggtt ctggtacttt
     6901 tcgccgcgtt cgagattgtc gacggcgatg atgtcggtga tgccgcgctc gttgagggcg
     6961 agaacgttgt tgcagccgat gaagccggcg gcgccggtga cgagaatgct catggcagtc
     7021 aatccttaaa agagttcttc ataagaaacg gtggcggtgc cgagcttgcc gacgaccacg
     7081 ccggcggcac ggttggccgt gcggacggcc tcctcgacgg tcatgcccgt ggcgagcatg
     7141 gtggcgagga tggcgatgac ggtgtcgccc gcgcccgaga cgtcgtagac ctcgcgcgcc
     7201 tgagtgggca cggtgatctg gccgccttca aagtaaaggg tcatgccttc ctcgctgcgg
     7261 gtgaggagga ggtacttgag gccgagctcg gcgcgcaggc cctgggcctt ggcggtgagc
     7321 tcctcctcgg tcctccagga gccgacgacc tggcggagtt cggagcggtt gggcgtgagc
     7381 atcgtggcgc cgcggtagcg cgagtagtcg tcgcccttgg gatcgatgag gacggggatg
     7441 cccgcatcgc gcgccatggc gatcatggag acgatgtggg agaggccgcc cttgccgtaa
     7501 tcggagagaa tcagggcgtc gtgttcggcg agcagggcgc ggaacttgtc gaggtgcttg
     7561 ttgaggacct cctcggcggg atgcgactcg aagtcgcagc gaagcatctg ctgttggcgg
     7621 gccacgacgc gcaacttgac ggtcgtgtcg agcgattcgt cgaactggaa gtgcggcgtg
     7681 atgccctgtt cgcgcgcaaa gcgttcgagc gtgcggccgg attcgtcgtt gccgacgacg
     7741 cagagcaggg aggagcggcc gccgacgctg gcgatgttga ccgcgacgtt ggccgcgccg
     7801 ccgaggcgct cttcggtgcg gctgatgcgc acaacgggaa ccggcgcttc gggggagatg
     7861 cgttcggcgt cgccaaacca gtagcggtcc agcatggcat cgcccgtcac gagaacgcgg
     7921 gctttggaaa gagtctgttt gtccataaaa cttgcgggcg gcgcaacggc cgccatggtg
     7981 gtagacgtgc cctgattgta atggttggcg ggccctcgcg tcggcgcggc cgtgcgtttg
     8041 ccaacggttg caatcgggtt ttgggcggaa gtcaccgatt ggctacaatt atctagttac
     8101 ctgaagcagg cgcaggcccc gttccagcgc ctgcggacta atacgaggag agaaattatg
     8161 gcgcccgacg cctctatttt taaggcttat gacattcgcg gcattgttga caagactttg
     8221 acggaggatg ccgtccgcca gatcggccgg gtgctcggca cgatggcgca cgaggccggc
     8281 aggacctcgt tctgcgtcgg ccgcgacggc cgcctgtcgg gcgagaggct cgcgggcgcc
     8341 ctgatggagg gcatcacttc cgcaggcgtg gacgtcatcg acatcggcat ggtgccgacg
     8401 cccgttctct acttcgccac cgtccacttc ggcaacggca cgggcgtcgc cgtgaccggc
     8461 tcgcacaatc cgcccgaata caacggcctc aagatgatga tcggcggcat cacgctcttc
     8521 gccgaccaga ttcagggcat tctcgcccgc atccggaacg aggactggcg cacggccgag
     8581 gtgccgggca cgatccgccg tgaggacgtg gtgcccgcct atctcgagcg cgtcctttcc
     8641 gacgtgcgtc tcgagcgccc aatgcgcatt gcgatcgatg cgggcaacgg cgtcgccggc
     8701 cccgtcgccg ccaggctctt caagtccctt ggctgcgagg tgaccgaact cttcacggac
     8761 gttgacggca ccttcccgaa ccatcatccc gatccctcca agcccgccaa tctcgaggac
     8821 ctcaagaagg cggttctcga ggacggcatc gagctcggcc tcgccttcga cggcgacggc
     8881 gaccgcctcg gcgtcgtgac gaagtcgggc gagatcattt atcccgaccg tcagatgatg
     8941 ctctttgcga aggacatcct tgcgcatcat cccggcgccc agatcgtcta cgacgtcaag
     9001 tgcacgcgcc gtctggtgcc ctggatcgaa gcctgcggcg gcgtcccgac gatcagctgc
     9061 acgggccact ctctcgtcaa ggccaagctg cgcgagacgg gcgctccgtt cgcgggcgag
     9121 atgtcgggcc atctcttctt caacgacggc cgctggcccg gctttgacga cggcgtttat
     9181 gccggcgccc gcatccttga aatcctttcc cgcgaggcgg atccgtccgc cgcgcttgaa
     9241 gcgctcccga gcgccgtcaa cacgcccgaa ctgcagatcc gcatggccga aggcgagaac
     9301 aaggccttca tcgcccgcct gcagaaggag gcccgctttg acgacgcgct tgacgtgatc
     9361 acgatcgacg gcgtgcgcgt cgagtggaac gacggcttcg cgctcgcccg ctcctccaac
     9421 acgacgccgg tcgtcgtgct tcgtctcgaa ggcgacacgc ccgaggcgct cgagcgcatc
     9481 aagaagacct ttgcggaggt gctgctcaag gtcgagccgt cgcttgagct tcccttctga
     9541 tagaccgcgt caggaatttt cctcatgact gattcgaaca actggaccct gcttcccttc
     9601 ctgcgcgaca gcgcaggcgt ggacaccatg gtgaccgcct gcgggatccg ccttgacatc
     9661 tcccgccagc gcctgacggg cgaggacctc aagcgccttg tcgaccactg ccgcacgcgc
     9721 ggcgtcgagg aggcgcaccg ccgcatgatg gcgggcgaga tcgtcaatcc gagcgaaggg
     9781 cgcgcagcgc ttcacacttc gctgcgatcc ttttcggccg gtgcgccgaa gttcgccgag
     9841 gtcgaccgcg agcgtcgccg catgctcgac ttcgcgcgcc aggtgcgcga gggcatccgc
     9901 ctcggctgca ggggtgaccg catcaccgac gtgatcaacg tcggcatcgg cggctccgac
     9961 atggggccgc acgcggtcta ccatgcgctt cgtccctcca atccgaagat ccgcgtccat
    10021 tttctctcca ccgtcgacgg cgtgctcctt gagcgcattc tcgacgtctg caatccgctg
    10081 acgaccctcg tcgtcgtctc ctcgaagagc ttcggcaccc gcgagacgct cgtcaacgcc
    10141 gcagccgtcg accagtggct gctcaaccac ggcatcgtgg gcacggaccg tgcgcaccac
    10201 atggtggtcg tctcggccaa ccccgacgcg gccgcccaga tgtgcctgcc cgaggagaac
    10261 ttcttccgca tgtgggactg ggtcggcggc cgattctccg tctggggcgc agtgggcctg
    10321 ccgctcgcac tctctctcgg gcccgaggtt ttcctcgagt tcctgcaggg cgcggccgaa
    10381 atggaccgcc actcgctgac ggcgccgctt gagaagaacc ttcccgcgac gctggccatg
    10441 attgcgttct ggaacgccaa ccgcctcgag atcagcacgc actgcgtgct gccctacgac
    10501 gagcgcctgc gcgtgatcgt gccctggctg cagcagctcg agatggagtc gctcggcaag
    10561 aaccacacgc ctgacggccg ccgcatcccc ggcaggaccg gccaggccgt ctggggcgca
    10621 aacggcaacg aggcccagca cagcttctac cagtggcttc gcgaaggcac gggccgcgcc
    10681 agcatcgatc tcctctggag cgagatgccc ggccaccgct atgcggagca ctaccgcgtg
    10741 ctgctcgcca acgcccgagc tcaggccgag gcgctcatca tgcgcgatcc cgacaatccc
    10801 tgcttcaacg ccgtctcagc catcgtgatg gacgccgtga cgccgcgccg cctcggcgcg
    10861 ctcatggcca tgtacgagca caagacgacg atgctcggca cgctcttcgg catcaacccc
    10921 ttcgaccagc ccggcgtcga gcttggcaag cgcctgagca agcgcgccga acgtggcgag
    10981 gatccgatgg ctgcagttgc ggaagaggtg aggttttaat tgaagatcct cgtcatcaag
    11041 tcctcctcca tgggcgacat cattcatgcg ctgcccgtgg ctcatgacat caggcaggct
    11101 ctgcccgagg ccgagcttca ctgggttgcg gaagagagct tccgcgacat tccgacgctc
    11161 gcccccgcag tctccaaggt tcacgtgacg gccttccgcc gctggcgcaa ggccattctc
    11221 tcgggtcccg tccgagccga aatggcggac gtgaagaatg cgcttcgcgc cgaacactac
    11281 gacgtcgtgc tcgacatcca ggggctgatg cgctcggcga tggtcgcccg ctgggcgggc
    11341 gttcccgcca cgggctacag ctggggcacg gcccgcgagc cgatcgcctc gctcgcctat
    11401 gcccgcaagc ttgatctgcc cgcatcgctc ggcgccgtgc gccgctaccg catggccgca
    11461 gccgaagctc tcggctacga gatcgatccc gaaaggcccg tcttcggcct gcaggcgaat
    11521 gcggagccgt ccgtgaagcc cgccggtccc tatgcggcgc tggccgtcaa tacgagccgt
    11581 gacgaaaagc tctggcccga ggagtactgg acgcatctgg gccgcaagtt cgccggcgca
    11641 ggcctcagaa gcgttctctt ctggggcagc gacgtcgagc gcgagcgcgt ggagcgtctg
    11701 gtcaggacca ttcccggcgc cgaagtggct ccgaggacca ctctggcgac gacggccgcc
    11761 acgctggccc gtgccgagtg cgtcgtgggc gtcgacacgg ggcttgccca tctcggggcg
    11821 gcgctcggtc gtccgagcgt cggcatcttc gtctccacgc cgaccgagac cctgaagctt
    11881 gtcggggacg gtcccgtgcg cagcctcggc ggcatcggac agtgtccgga cgtcgacgag
    11941 gtttggaagg cgctcgtcgc cgttcaggga gaacgcttgt gagaatcact ccgtccatgt
    12001 acagggcggt gagcgtcgtt gcgctcccgc tcgccagtct ttatctgatg tggcgctccc
    12061 gccgccagcc cgcttaccgc cagttctggg acgagcgctt cgcctggtcg agctttccgc
    12121 tcaagacggg gcgtccgcgc gtctggatcc acgccgtgag cgtgggcgag acgaatgccg
    12181 ctaagccgct gctcgaggcg gttctcgagc gctggcccga gtgcgacgtg ctgctcacgc
    12241 acatgacgcc gacgggtcgc gaggccggcg ccaagcttgt ggcgcttgcg cccgaccgca
    12301 tccatcagtg ctatctgccc tacgacgcgc cgtatgccgt cgagaagttc ttccgccaga
    12361 cccagcccac gatcggcatc atcatggaga ccgaggtgtg gccgaacctc atgagcgagg
    12421 ccaagcggtg cggcgtgccg atggtgcttg ccaatgcgcg cgaaagcgag aagtcccgcg
    12481 ctcaggccga gaagttcctc gacgtgatgg gccccgcctt cggcagcttc acggccatcc
    12541 tcgcgcagag cgaggaggac aaggagcgtc ttgagagcct tggcggacgc aacgtcaccg
    12601 tctgcggcag cgtcaagttc gatatccgtc cgaacgcctc ccaggaggcc gccgcgcgtg
    12661 cctggaaggc gtcgctcagc cgccccgtcg cgctcatcgc ctcgacccgt cagggcgagg
    12721 aggtgctgtt tgcgaaggcc atggccaccc gtcccgaact tctcaggcgt gcgcagttct
    12781 ggctggtgcc gcgtcatccg cagcgcttcg acgaggtctt ccgcacgctc accggcgcag
    12841 gcctcaaggt ctgccgccgc tcgcagattg cggagcccgc cgacgtgccc gccgacaccg
    12901 acgtcgtgct cggcgacagc atgggcgaaa tgagcttcta ctgcgctctg gccgacatga
    12961 cggccatggg cggatccttt cagaaccacg gctcccagaa cgtgatcgag cccgcgcttg
    13021 caggctcgcc catcgtcgtg ggaccgtcca tcttcaactt tcagaaggtc atccgcgacg
    13081 gcctgtccgc tggcgccatg gtgcaggtcg agactcccga ggaggcactc gaccggttcg
    13141 agcactggct tgacgttccc gaaacccgcg tgagcgcgag cgaggctgcc ctcggctttt
    13201 cgaggcagta tgcgggcgcg actcagcgaa tgatgacgat attggaggtc ttgtggcaga
    13261 aggcacagaa aacagcatcc ctaacgtcgt aagcatcgcg ggggtggacc cctcgggcgg
    13321 tgcgggcgtt ctcgccgacg tcaaggccat gagcgcctgc ggcgcctatg cctgcggcgt
    13381 catcgcggcc ctgaccgctc agaacacgca gaccgtcacg ggcgtcatgc ccgtgcccgc
    13441 gaccttcctc aaggagcaga tcgacacgct ctttgacgac gttcgcatcg acgcggtcaa
    13501 gatcggcatg ctcggcacgg tcgagcagat cgagacggtg gccgacgcgc tcgtgcgccg
    13561 caagccccgc ttcgtggtgc tcgaccccgt catggtcgcc aagggcggca gccggctgct
    13621 tgcggctgac gccgtcgacg ccttgaagga gaggctcctg ccgctcgcca ccatcatcac
    13681 gcccaacctg cccgaagcca tgtgccttct cggacgagaa gaggacgtga cggaggagga
    13741 gttccccaaa gtcgcccgcg agctctggaa gctcctcggg cgtcgaaacg cctgggtcta
    13801 tctcaagggc ggccatcttg agggcgagtc cgcctacgac ctgctcttca acaacgtcgt
    13861 ctcggttccc ttcacggctc ctcgcgtacc gacgaagaac acgcacggca cgggctgcac
    13921 gctttcgtcc gccatcgcgg ccttcctgcc gcagtcggtc gacgtgcccg aggcctgccg
    13981 ccgcgccaag gagtacctga cgggcgccgt ccgacacgca ggcgagctcg acgtcggcca
    14041 cggccacggt cccacgaacc acttctggcg cctctggaag gagagggtgt aaaaatttgt
    14101 aacgtctcct tgacggggga ttgggaaggc tgcataatct caatcctcaa cggcgcatta
    14161 gctcagtcgg ttagagcaga ggaatcataa tccttgtgtc cgcggttcga gtccgtgatg
    14221 cgccaccaga tttcaaaagc cccgccttct ccactgaagc gcggggcttt tttctttccc
    14281 ggccgcaggc catggtcctg cggctctaaa catcgtcccc ggatctaaac cgtgcaagcg
    14341 aagaaactct atctgcgcag cttcggctgc caaatgaacg actacgacag cggtcgcatc
    14401 gccgaccttc tcaacgccga aatgggctat gaacgcaccg acgtgcttga ggaggccgac
    14461 ctggtcgtcg tcaacacctg ctccattcgt gaaaaggccc aggagaagac tttctccgac
    14521 ctcggccgca tccgcgaagt caagaagacg cgtcccggca tgctgatcgc cgtgggcggc
    14581 tgcgtcgcga gccaggaggg cgaaaacgtc atccgccgcg cgccctgcgt tgacgtggtc
    14641 ttcggaccgc agaccctgca tcgtctgccc gacatgcttc gcgcccgcgt tgagacgggc
    14701 cgtccccagg tcgacgtgag cttccccgaa atcgagaagt tcgatcatct gcccgccccg
    14761 aaggcgcatg gcgccgccgc cttcgtctcg atcatggaag gctgctcgaa gtactgctcc
    14821 tactgcgtgg tgccctacac ccgcggcgag gagatctcgc gcccgatggt ggacgtcctc
    14881 atggaggtcg cccagcttgc cgatcagggc gtcagggaag tgacgcttct cggccagaac
    14941 gtgaacgcct atcgcggcaa gatgcccgac ggctcgattg cggacttcgc cctgctgctt
    15001 gactacgtct ccgaaatcga cggcatcgag cgcatccgct acacgacgag ccatccgcgt
    15061 gaattctccc agcgcatcat cgacgcctac ggccgcctgc ccaagctcgt caaccacgtg
    15121 cacctgcccg tgcaggcggg ctccgaccgc gtgctcgccg ccatgaagcg cggctacacc
    15181 gccgccgagt acgtcgagat cacgaggaag ctccgagagc ttcgtcccga catctcgatc
    15241 gcgaccgact tcatcgtggg cttccccggt gaaaccgccg aggacttcga ggacacgatg
    15301 aagctcgtcg agaaagtggg cttcgacgcc agcttctcct tcatctacag cccgcgtccc
    15361 ggcacgcccg ccgcccgtct tcccgacgac acgccctatg acgtgaagct tgcgcgcctg
    15421 cagcgcctgc aggcgacgat cgacgcgaac gcgacccgca tcagcgagag catgctcggc
    15481 accgagcagc gcgtgctcgt gctcggcccc gcgcgcaagg gcgagggctt cctcatggcc
    15541 cgcacggaca acaaccgcat cgtgaacttc gagggaccgc tctcgcttgt ggacacgatg
    15601 gtgcgtgtta aagtgacgga tgtgtatccg cattcgcttg gcggacaact tctggactga
    15661 ctaccgacct atggctagtg aaaagaccgt gcagcccgaa acgctgcgct actccgcaga
    15721 tgcgggaaac gccgagcttt ccgcgctctg cggcccgctt gacgaaaacc tgaggcagat
    15781 cgagacgatc ctcaacgtgc gcgtcagccg ccgcggagac gtcttccgcg tgacgggcga
    15841 gcttgaggcc gcccgccgcg ccgtgacggc gctcgcagtc ctgatgaccc gcgtcaagag
    15901 cacgggcgag gagctgagcg tcgacgacat ccagtggcac ttcgtcggac atgacgacga
    15961 gtaccgcagc ggcgccgcgt ccgatcccga gggcgccgtc gacgacgtcg acctcaaggt
    16021 ccgccgccgc gacctccagg gccgcacgcc caaccagcgc ctttacctca aggccatcat
    16081 ggggcacgac atcagcttcg gcgtcggtcc cgccggcacg ggtaagacct tcctcgccgt
    16141 cgccgcggcc gtcgacgcct tcgagcgcgg caccgtcgag cgcatcgtgc tcacgcgccc
    16201 cgccgtcgag gcgggcgagc gtctcggctt cctgcccggc gacctgacgc agaaggtcga
    16261 tccgtacctg cgtccgctct acgacgcgct cttcgatctg atgggcttcg agaagtcaca
    16321 gcgcctcatg gagcgccagt cgatcgaggt ggcgccgctc gcctacatgc gaggccgcac
    16381 actcaacaat gcgttcgtca tccttgacga ggcccagaac acgacgcccg agcagatgaa
    16441 gatgtttctc actcgcatcg gctttggttc caaggctgtg atcacgggcg acgtgacgca
    16501 gatcgacctt ccgaagggcg ttccgagcgg cctgaagcat gcgatccgca ttcttgacgg
    16561 cgtgcgcggc atcgagatca cgcgtttcac gtccaaggac gtggtgcgcc atccgctcgt
    16621 cgcgcgaatc gtcgacgctt atgaagaggc cgccaagcag gatgagcttg cggccaaggc
    16681 cagaaaagag gtgaaagaat gagcgaacct gcagtgaagt tttcgttcca gaacgcggcc
    16741 gctttcccgc agctgccttc gcgctccgtg atgattcgct ggatgacgcg cgcctgcgag
    16801 cttccgacga tgatgacggt ccgcttcgtc gaccaggagg aaggctacga gctcaaccac
    16861 tcgtaccgcc accgtgacta cgcgacgaac gttctgacgt tcaactacac gaccgagccc
    16921 gaggtgaccg ccgacgtggc gatctgcgtg cccgtcgtcg agcgtgaggc agcggagcag
    16981 aacaaggagt tccgcgcgca tctcgcacac ctgctcatcc atgcggttct gcatgcccac
    17041 gggtatgatc atgttacgca ggaagaggct gaggtcatgg aggctcgcga aaccgagctc
    17101 atgctctccc tcaagttccc caatccctac agcgaccgca tcggcatggt gcacgactga
    17161 ctctgaccaa gttgaaaaca agctgttccg gcaaatatgt ccagttcctc tgaacctccc
    17221 agtccgacct tgggccgtac ttttcttgac cgtcttacct ccgtcttcca cgaagaagag
    17281 ctcaacgacc gggccgacgt gctcgaagtg ctcggcgagg cccgccgccg caatgtgatc
    17341 gacgacgaag tgttctccat gatggagggc gcgatggccg tttccgaaat cagggcgggc
    17401 gacctgatgg tgccccgctc ccaggtgcag gccgtcgact tctcggagcc gcgcgaagtc
    17461 tggcttcccg ccctcattgc ctcggggcac tcccgtctgc ccgccgtcga ggacgatctg
    17521 gacaacgtgc tcggcatcct gcacgccaag gacctgctgc aggtgcttat gaatcccgcg
    17581 gttgatccga agacgcttct gcgtcccgcc cgcttcattc cggaaacgca gccgatcaac
    17641 gtgctgttgc gagacttcaa ggagacccga agccatctgg ccctcgtcat tgacgagttc
    17701 ggcagcatct ccggcctcat caccatcgag gacgtgctcg agcagatcgt gggcgagatt
    17761 tccgacgagt tcgaccatga cgaagagggt agcaacatcg tcgccgacgg cgagggccgc
    17821 tggcgcgtca aggccatcac gccaatcgag cagttcaacg actacttcaa gtcgaacctc
    17881 gaggaccagt actgcgaaac gatcggcggt ctcgtgaccg accgcttcga gcatgtgccg
    17941 cacacgggcg agaccatcga ggagaacggc ttccgcttcc gcatcctcgg cggcgacgac
    18001 cgtcaggctc gtctgctgag cgtcgagagg atcaagtagc gtgagcgagg cgtcgcactg
    18061 gtgggagcgc cggcccgaag cggcgggcgc gctcggcgcc ttgggcatgg ggcttctctt
    18121 tggcacgggg tttgatccgc ttggctggca gtggatgtcg ctcatcgcgc tcgtcggctt
    18181 cttcctcgtc ttcgcggcgc gtccgacgcc cgcccgagcc gcgctcgtgg gttttctctt
    18241 cggactcggc tggttcgtgc cgggactctc ctggacgatg aactccattg cggtctacgg
    18301 gcgcctgccc tgggccgttg cggcgcttgc gcttcttgta ctgggcgccg tcctttctct
    18361 ctttcccgcc ttcgcctgct ggattgcgaa gaaaatcggc ggcggaaggc tcgccaccga
    18421 gctgcccgcg ctcgcaggcg tctggaccct tgcggaatgg ctcaggggcg acgtgctcgc
    18481 caatttcggc tggctcatgc cggggtacgc cgtcatcgac acgccgcttg cgggctgggc
    18541 cccgctgggc ggcatctacg cggtcacgct cgcacagctt ctcttcgcgg cgctcgtcgc
    18601 cgtcgcgctc agctgtcgcc ttgcctggct tgcggtgcct gtcatcggtg cagcgctcat
    18661 tgctggcacg gggcaggtct cccggcagca cgcctggtcg gagccgtcct ccacggtgga
    18721 cgtgcgcatc attcagcccg cgctgcccgt ggtcgacgcc tatacgcgcg ccaatcccgc
    18781 acagcgcatc gaggcgctct ttcccgccgc cttcaagccg tggcccgcat ccgacaggcc
    18841 ccgactgctg ctgacgcccg agggcatcgt gaacgtgccc gtgagccgtc tcggcaggga
    18901 ggcggcaggc aggctcgtgc tccttcagga gaagaccgag gcaagggtgc tcttcaacgg
    18961 cttccgcaag gacggtccgc gcttcttcaa cacctccttc gttctggacg aggggcagat
    19021 cgcctacagg ctcgacaagc gtgagctcgt gcccttcggc gaatacgtgc ctgcaggctt
    19081 ccactggttc gtcgagatga tcggcattcc gatgtcggac ctgatgcggg gcgacgccgt
    19141 gcagccgctc ctgtcgctcg gaggggccga tgcgggcatt ctcatctgct acgaaaacct
    19201 ctacggcagc gtggtccgca ccttctggca gtcccgctcg cccgacttcc tcatcgtgac
    19261 gtccaacctc ggctggttcg gacgctccgt gctcggacag cacctcacga tgagccgcat
    19321 gcgcgcgata gaaagcgcaa ggccgctggt gagcgtgagc aacacgggca tgagcgccct
    19381 cgtcaattcg aggggcgaga tcgcggccat gctcaggacc gatggtcccg atgccgcgac
    19441 catgccgctt ctgggcgcca cgggctcgcc cacgccctat gtgaggctcg gcagctggcc
    19501 cgcgctgctc gcatcgctcg ttctggcgct ctgcgccgtg ctcgtccgct tagggacagc
    19561 cagaatccga gcgggcaagg taaagggctt ataattaaat ctttgagctc ccgtttccgt
    19621 caggaaaggg agggctccct cattttttga atcaacaggc gccgacaaga atcggcggca
    19681 tagagaatga ttacctttca ggaagtaatc ctgcgcctgc aggaatattg gaaccgccag
    19741 ggctgtgctt tgctgcaacc gatcgacttg gaagtcggtg ccggcacgtc ccatactgcg
    19801 acctttctgc gggctctggg gccggaaccc tggcgtgccg cctacgttca gccctcgcgt
    19861 cgtccgaagg acgcccgcta tggtgaaaat ccgaaccgcc tccaccagca ccaccagttc
    19921 caggtcgtgc tgaagccggc tccgacgaac atcgtcgacc tctatctggg cagccttcgc
    19981 gcgctcggca tcgacacgga agtcaacgac atccgcttcg tcgaggacaa ctgggaaaac
    20041 ccgacgctcg gcgcttgggg tctcggctgg gaaatctgga tgaacggcat ggaagtgacg
    20101 cagttcacgt acttccagca ggtgggcggc cttgaatgca agccgatcac cggcgaaatc
    20161 acgtacggtc tcgagcgcct gacgatgtat ctgcagaact gcgacaacgt cttcgacctc
    20221 gtcttcacga cgtggaccga tccggacggc acggaacgca agctcaccta cggcgacgtc
    20281 ttccatcaga acgaagtcga acagtccgtc tacaacttcg aggccagcga ctgcgagaag
    20341 cttctcaagc agttcgacta cttcgaaagc gaagccaagc gcctgatgga cctgaacctg
    20401 gccctgcccg cctacgaaat ggtgctcaag gccggccaca ccttcaatct gcttgacgcc
    20461 cacggcgcca tctccgtgac cgaacgcgcc gcctacatcg cccgcattcg caacatctcc
    20521 cgctctgtgg cccagagcta ctacgattcc cgtgaacgtc tgggcttccc gatgctcgcc
    20581 aagtccgcgg aataatgcga gtggaggaaa agaagaaaat gacgaatttg ctcgttgaac
    20641 tgcaaaccga agaactcccg ccgaaggccc tcgaaaagct ttccgaagct ttcgccgccg
    20701 gcgtcgaaca gcatcttagg aaccgcggct tcctcgccga aggatccgtc accacggcct
    20761 acggctctcc gcgccgcctc gccgtgcaca tctcgaacgt gcttgaaaag agccccgacg
    20821 aagccttcac gcagaagctc gtcccggtgc gcgtcggcct tgccgccgac ggctccgcca
    20881 cgccggctct cgtcaagaag atgaacgccc tgggcatcac gtgcgacgtc gccgagctca
    20941 agcgcgtcaa cgacggcaag aatgaacagc tcgtctttga aggcgtccgt ccgggcatcg
    21001 tgctcgccga aggcctccag accgctctcg atgccgccgt caagaacctc ccgattccga
    21061 aggtcatgac ctaccagctc gccgacggcg agacgacggt cgccttcgtc cgtccggtca
    21121 agcatctcac cgccctctac ggcgaacaga tcgttccggt caagctcttc ggtctcaacg
    21181 caggccgcat cacgcgcggc caccgcttcc acacgacgga accggtcacg ctctcctgcg
    21241 ccgacgccta tgacgccgag ctcaaggccg ccaaggtcat gccctgctac gccgagcgcc
    21301 gcgaactcct tcgcaagctc ctcacggagc gcgccgccca gatcggcggc acggtcatca
    21361 tgccggaaga ccttctcaac gaagtgaccg ccctgaccga atggccgatc atctatgaaa
    21421 gctcgttcga agaagagttc ctcgccgttc cggaagagtg cctcatcctc acgatgcagc
    21481 tcaaccagaa gtacttcgcg cttcgcgacg ccgaaggccg cctgatgaac cgcttcctcc
    21541 tcgtctccca gcttgaggcc aaggacggcg gcgccgccat ctcttcgggc aatgcccgcg
    21601 tcgtgcgcgc ccgtcttgcc gacgccaagt tcttctacga tcaggaccgt caggaaaagc
    21661 ttgagacccg cgttgacggc ctcaagcacg tcgtctacca caacaagctc ggcagccagg
    21721 ccgaacgcat gctccgcgtc aagacgatgg ccggcctctt cgccgacctg atcggcgccg
    21781 accgcgccaa ggccgaacgc gccgccatgc tcgccaaggc cgacctccgc acgctgatgg
    21841 tcggcgagtt ccccgaactc cagggcatca tgggcgagta ctacgccaag tatgacggcg
    21901 aagcagagga agtggcgctt gccatccgcg agcactacca gccgcgctac gccggcgacg
    21961 ccctgccgtc caccccggtg agcctcgcca cggccctcgc cgacaagatg gagaccctga
    22021 tcggcctctt cggcatcggc cagatgccga ccggcgaaaa agacccgttc gcgcttcgcc
    22081 gccacgccct cggcgtgttg cgcatgctca tcgaaaaggc cctgcctgtc agcctcaaca
    22141 cgctgatcga tgccgcctgg gaggctgaaa aggctgttcc gggcgtgacg gaccaccgtg
    22201 aagagcttcg ccagttcttc caggaccgcc tgcgcgtcat gctccgcgat gccggctaca
    22261 ccacgctcga agtggacgcc gtcctcgccc tgaatccgga aaagctcgac gagcttccga
    22321 agcgcctcgc cgctgtccgc gccttcatgg cgctgccgga agccgaagcc ctgaccgccg
    22381 ccaacaagcg catcggcaac atcctcaaga agatcgaggg cgaagtcccg acggtcgtca
    22441 acgaggccct tcttgccgaa gacgctgaaa agcagctctt cgccgccatc aacgacgtcg
    22501 aacccaaggc ccttcagcac ttcgccgccg gcgaatacga gcagatgctc gcgacacttg
    22561 ccgttcttcg cggcccggtc gacagcttct tcgacaacgt catggtcaac gccgaggatc
    22621 cggccctgcg cgccacgcgt caggcccttc tgaagcgcct ctacgacgtc atgaacaagg
    22681 ttgctgaaat cgcccgcctc gccaagtaag cgacgtgcgc cgaaacggcc ctttcgaggg
    22741 ccgttcaaca gggatccaaa acggtatgat ttcgttttgg atcctttttt tatccggctc
    22801 tgcaggccgg acattccaac atgaatcttc ttcgaggctg gctcttctac atcgtcctga
    22861 gcttttcgat tctggtcctt gcggtctgcg tgctctgcac gacgcccttt gcgagcgtga
    22921 agtggcgcta tgaggccatc tgccgtccgt gggcgaagtt cgtgctcgca ctgcttcgcg
    22981 tcatctgcgg catcaggctc gagatgaagg gcatggagaa ccttccgccc gccgacacgc
    23041 aggcggtcgt gctgatgaag caccagtccg cctgggatcc gttctggctc ggcgcctatc
    23101 ttcccgcgcc cgcctgcttc atctacaagc gctcgatcaa ctggattccg gtcctcggct
    23161 gggtggtctg cgccatggac atgctggcga tcgaccgcag caagggccgc tccgccttcg
    23221 aggccttcat gaagaagggc cccgtgaagc tccgcgaggg cttctggatc agtctcttcc
    23281 ccgaaggcac ccgcgtgccg ccgggagagc gcgtgcgcta caagacgggc ggcgcccgct
    23341 tcgcatgcca gagcgggacg cccatcgtgc ccgtctgcca caatgcaggc cactgctggc
    23401 ccaagaactc catcgccaag caacccggca cgatcaccgt gagcgtcggc cccgtcatcg
    23461 agacgaaggg cagggatccg catgaggtga cggccgaagt cgaaaaatgg attgaagatg
    23521 agcttgaacg aattgatgcc tgaagccggc agggggctgt ctcttcgatt gacgtcgcac
    23581 cagagggccg tgacggcgga agacggcgag cgcatcgaat atctgttcca gccctcccga
    23641 cgccacagga cccgcgtcat cctgagctac gatggcaggc aggacaagcc cgtggtgacg
    23701 gttccgtacc gctatgccgg tccgcagatg ctgagcgatg aattcgttct tcagcggtgc
    23761 gatgaaatca ttcttcagaa gagggagtac gtcaggtgga tgcgggcgca gacgcatgca
    23821 gttctggacg ctgcgtactg cattgcaggc ggcagcgggt acattctcta ccgcggcagg
    23881 aagacggccg ttgcggtcga cgcgacggcg aagacttcgt acgtcgacgt gacgggagac
    23941 gacgccgtca ttcgtctggc gctgccgcag acatgctcga ggaaggaggt cgacagggcg
    24001 ttgctagccc ttttcaaggt gcaggccaaa acggtgctgg gcgacctgat ggaggcctat
    24061 cgcggcagtg cgcgccactg gcccgcgtcg tggagtctct caagcgcgag gatgagatgg
    24121 ggatgctgta cgacgaggaa tacgatcagg ctctgttgga ggctgatctt ccttgatgat
    24181 gcggtcgcct cctacgtgat cgcgcacgag cttgcgcatc tcgagcattt caatcactcg
    24241 aaggccttct ggaaggaggt ggagaggctc tgcccggact ggcgggtaca gctcgccaaa
    24301 ctcaacgcgg cgaaggtgct gcccgaactg gaggaggtcg ggcagtgagg ctcagacggc
    24361 gtcggcgtga tgcggcagat tggccgcctc gcgtgccagt cgcacgaagg ccgccacgtc
    24421 gagcgtttcg gcgcgtgcgg cgggattgac gcccgcgcgt ttgatcgcgg cgtcatccat
    24481 gagggtggag agcgcgttgc gcagcgtctt gcggcgctgc tggaaggcga gcgcgaccac
    24541 ttgggagaag atctccatat cgacttcggg aagcgactcg acggggcgcg gcaccatgcg
    24601 tacgatcgag gacgtgacct tgggcggcgg cacgaaggcg ccgggcggca cgtcgaagag
    24661 cttgtgcatg acgtagcggt actggagcat cacgctcaga cggccgtaag tcttgctgcc
    24721 gggttcggcg accatgcggt cgaccacttc acgctggagc atgaagtgct ggtcgatgac
    24781 gcggtccgca gcgctcatga gcgcgaagag gagcggcgag gagatgttgt aaggcaggtt
    24841 gccgatgatg cgcaggcggc ggccctcaag gacggtcgtc cagtcgagtt tcagggcgtc
    24901 ggcctcgatg agcgtgagct cctcggggga gaactgctcg cgcagaaact gggcgaggtc
    24961 gcggtcgatc tcgacggcgg cttcgtggcc tgcgcaggcg atgacttcgc gcgtgagcgc
    25021 ggcctggccc gggccgattt caatgagagc gtcgcccggc ttcgggtcga tgccgcgcac
    25081 gatgcgctcg atccagtggc ggtcatgaag gaagttctga ccgaagcgtt tgcgggcctt
    25141 gtgcccttca agccattctt gtttcacggt tcttcgataa aaagtgtgct aatcggaggg
    25201 cggagatcat gctcgtgtcg tccgccttgc cctgtcctgc gatgtcgagc gcagtgccgt
    25261 ggtcgaccga ggtcctgatg tagggaagcc cgagcgtcac gttgacgccc tcggcgaagc
    25321 ccacgtgctt gaggacggga agtccctgat catggtacat gcagagcacg gcgtcgtagc
    25381 ggtcccagtg gccgggcacg aaggccgtgt cggcaggcac gggaccgtcg gcgtgaatgc
    25441 cgcggctgcg cgctctttcc agagcgggcg tgatcgtgtt gatttcctcg tggcccatgt
    25501 ggccgttttc acctgcgtgc gggttgaggc ccgtgacgag gatgcggggc tcggccagac
    25561 cgtagtcgcg tgcgagcgcc gtgttgagcg tgtcgagcac ctcgtcgagc agctcgcccg
    25621 tgatggcctc ggaaagcctg gcgatcggca ggtgagtggt ggcgagggcc accttcatcg
    25681 cgtccgtgcg ggcgttcgac gtgagcatca tgacgacgcg cctgacgccg cagagatcct
    25741 ggaagaactc ggtgtggcct gtgaagggca caccggactc gatgatgatg cttttctgaa
    25801 cgggcgccgt gacgacggcg ctccattcgc ctgcggcggc gccttcatga gctgcgcgca
    25861 ggatgtcgag cacgtaggcc gagttgcggg tgtcaagcct gccgggttcg gcaggcgccc
    25921 gaagctgcac gtgacggatg gagacgtgcg cgggcagggg ccaggcgatg ccgaggcggc
    25981 gggcctcggc ttcaagcagc gtgcggtcgc cgatcaggag gatcgggtcg gacacggcgg
    26041 ctgcggcctt gagcgagact tccggtccaa tgccggcggg ctcgccggtc gtgacggcaa
    26101 cggaccggag ttcagtcatg gttcgcctca gtagtggctg tcacggattt caatgtaggc
    26161 gcggtcgcgg agctcgcgct gccagttgta gacggcctcg ccgagcttct tctcgcgaag
    26221 ctgctgtcgg gcggcgatgc gcatgcgctg cggattgccg ttcttttcca tgcggcgctc
    26281 gatcacctgg atgaggtggt agccgaactg ggacttgatc ggttcggaga cctcgttggg
    26341 cttgagcgag ttcatggcct tttcgaattc gggcacggtg tcgccgggct ggagccagcc
    26401 gaggtcgccg ccgcgggtcg cgctgttgtc gaccgagtgc aggcgagcca tcgtggcgaa
    26461 gtcggactcg cccgcgtcga tgcggcgctt gatgtcgttg aggcggtgga tcacctcgcc
    26521 ttcgggcgtg atgtcgctca cgagcatcag gatgtggcgg gcgtgggtct gttcgaccgg
    26581 gccgccggcg agcttgaact gaacgccgtc acgggtgtcg gagacgcgca tgacgtccca
    26641 ggagtccttg cccttgatga tgtagacgcc tttcctgggg gattccttga gcacgtcggc
    26701 catgacgttg ggcagcgact gcaggttgcg ccagccgatg tcaccgccct gcagcgcgtc
    26761 ggaggcgcgc gagtaggtgg cggcgagcat ggcgaagtcc tcgcctgcgc gggcgcgctc
    26821 ggcgatctcc tcggcatgct tcttgagctt ttcggtgttg tcgggatggt cgatcgggat
    26881 caggatgtgc tggatgtgga attccgtcgt gttgcccgtc gtgaagccgg cctgctcggc
    26941 gaggtaggcg tcgatttcac tttcgggaat ctggatcttg gcgtcgacct cgcgttcgcg
    27001 caggcgctgc gtcgtgattt cgtcgcggat ctcctcgcgg aaggaggaga aggagacgcc
    27061 gtcggccatc agacgctgac ggagctggtc gaccgacagg ttgttctgac gggcgatctg
    27121 ctcgatggag gcgttgacca tctggtcgtc gatgcggatg ccggtttcgc gggcgcgctg
    27181 cttgatggcg cgttcgaaga tcaggcgttc aagcacctgg gcgcggagct gctccatcgg
    27241 cgggagctgg atgttctggc ggcgcaggtt gatggcgacg acgtgcgtgc gctcctgaag
    27301 ctcgcggtcc gtgatgacgt cgttgttgac gaccgccacg atgcggtcga gttcgacgtt
    27361 gccggcctgc ggctggacga cgggagccgc ctgcgcagat tcggtaacgg cctgctcggc
    27421 ggcgaaggcg gtgctgaggg cggccatgct catcgtcagc acggcaatgg tcttgagctt
    27481 attcatagta atcgtactgt ccaatcttgt cgggaacacg ggtggtggtc tgataaccct
    27541 tgatgttcct ctggagttca gaaagcgggc ttgtgccaat actacccaag ccgttgagct
    27601 cgagttgtaa gaagaagttg gtctcgtcgt tgttggacgt cgtcgtgtag cgctgtgcgg
    27661 caaaacgaag cgtccagcag tcgtgcaggt actcgacgcc gccgatcatc tcgatgggct
    27721 tgtcctgcag gagcgagtag ttgtagcgga agaggccgaa cagacggttc gtgatcggcc
    27781 actggaccgc caggtcgatc tgcttgatgt agtcgttctg atagtccgtg tagtcgcgct
    27841 cggacgagta gttgtagcgg tagtagaggc cgacgaggga catcgggcgc ggctgccacc
    27901 tgacgcctgc cttggccttc acccagcggc tcatcgccga ggagtactgg ccgctcacgt
    27961 ccgacgtgat cgagcgcgtc agacgcgcgc ccacgctcgc gagcaggtcg ctgcgaacgt
    28021 cggtgcgaag cgggctttcg cgcatctggg tgtcgcccgg tgtgtagaag ttgacgcgct
    28081 ggtccgagaa gtagttgcgc tggcccacgc tggccttgaa gagctcgagg ccgctcgtct
    28141 tgtcgatgta gcgggtggtg agcacgctcg tcagctggtt ggcctcgctc acgcggtcgt
    28201 agccggagaa gatgttctcc gagaagagcg ttgcaaagtt caggtcggcg atcgtcgtgt
    28261 cgaagacggg caggtcatcc tgattgcggt acggcacgta cgcgtagtag acgcgcggct
    28321 cgagcgtctg gtacgcctcg cggttgaacc agacggcgtc gcggtcgaag acgaggccgg
    28381 cgtcaaggct gaacatcggc gtcgtgcggt gcggatcctt gttggtgaag cggcccccgt
    28441 cgcggtccag gttctcgagc tggtaccagg tgccgaggaa ggtgcctttg ggcacgacga
    28501 accagcccgc gtcgcgcatc ggataggaga ccgtctgctc gaagacgaag cggtcgcccg
    28561 tcgtgaacgt cggatgctcg aacctcgtcg cctcgatggt cgtgccgacc tcgaagccgt
    28621 agaagtcgcc gttgtagccg ttgaaggtga tctggggcac gcgttcgtac ggcttgtagt
    28681 agtcgatgtg gtcgaaggtg agcgcctggt tcttcttgac gttgatcgac gtgttccagt
    28741 acgtctcgtc gtagcgaagg gcgtattcct gcggcaggac ggtttcggag gactctcgga
    28801 cgttgctcga gaagtcgctg atgaagtcat cgtcggagac gcggttgtag tcgacgaagg
    28861 cgctcagctt gtcgcggcgc cagctcatgt cgacgtgcag gctcgaacgg ctgtcgtcca
    28921 tctccttgtc gtcgggcatc agcgcgtagt cgactgtcgt ctcgaagttg ttgtagagga
    28981 acctggcctc ggtgtcgagc atgacgccgc gcttggtcat cagacggggc gtcagcgtaa
    29041 ggtcgtagtt gggcgcgatg ttgaagtagt acgggaccgc gaggtcgagg ccgcgggtcg
    29101 agctcatgcc gaaggtcggc acgaggaagc cggagcggcg ctcacggctc acggggaagg
    29161 agaaccacgg ggagccgaag acgggcacgc ccatgaagtg cagcgaggcg ttcgtgcccg
    29221 ttgccgtctg gtcgtactcg tcgatctcga gctcgttcat cttgatgaac caggcttcgt
    29281 cgtcgcgctt gcaggtcgtg accttggcgt cgtcaaagac ggtcgattca cccgagatga
    29341 agcggatgtt ctttgcgcag ccgcggatgt tgcgcggcgc atattcgtat tcggcgtccg
    29401 acatgtcgcc cgtgcgcggc gagatcttga agcgcatgga cggacccgag aaggaggcgc
    29461 cctggcgcga gatgcgcgcg ttgccaacgg cctcgacttc gtcggtttcc tgcgtgtagg
    29521 tgattcggtc gcccttgagg acggtgccgg cgcggcggat ctccgcgttg ccgtaaaggt
    29581 ggagctggtc ttcggggttg ccttcgatgc ggtcggcgga cacgaagctc gcggcgtccg
    29641 agtcgggcgt tgcgcgggac ggatcgagcg tcgtcacgga ttcgagggac accacgctgc
    29701 ctgtctgagc ggccggcgca atggaggtgt aggccatggc ggccagcgcg atgcttacgc
    29761 tgatcgcggt ctgtcgggga cgcgggaatg acatgaaaaa acggtgagag tgacagcctg
    29821 tgggcaggcc tataaaatga cgtcaagaca attgaaggat tataaagccc cgtcattccc
    29881 gaggggcgcc gcgcagtcca taaaatccct gcgggcttca gatccttcgg cactagagga
    29941 cgacgaaaac atgacgaaca gcgcggccgc ctccgccttt tggcagtggc tccagagcat
    30001 ttccccgaaa tatcagctcg atctgacgac ggtaagccgt gcaggcgcgg acgctggctt
    30061 caggtcctat caccgcattg ccgccgccga cggccgcagc ttcatcgcca tgaatgccga
    30121 gggcgagacg cccgagagct tcgcttcctt catcaaggtc gacggcctgc tgaggaacga
    30181 ggcgggcctc aacgcccccg aaatcttcga agccgacacg gaccatcgct tcatgctgct
    30241 ctccgacctc ggcagggaga cctatcttga cgtcctcaac gaggacaacg cccgcgaact
    30301 catggaccgt gccaccacgg cgctcgtcgc gtggcagaag atctcccgcc cgaacgtgct
    30361 tcccgcctac agccgcgagg tcctgcagcg cgagctcgag ctcttcccga cctggtacgt
    30421 gggccgccac cgcggcgtcg agtggaccga ggagcagcag aagtggtgga ggatgaccac
    30481 cgaggccatt ctcgacacca actgccgcga ggccctcgtc ttcgtgcacc gcgacttcat
    30541 gccgcgcaac ctgatggtct ccgatccgat gcccggcatc ctcgacttcc aggacgcgct
    30601 ctacggtccg ctcagctacg acatcgcctc gctcctgcgc gatgccttca tctcctggga
    30661 cgagagcttc gtgctcgaca ccacgatccg ctattgggag aaggcccgca aggcgggcat
    30721 ccccgtgccc gccgacttcg gcaacttcta ccgcgacgtc gagttcatgg gcgtgcagcg
    30781 ccatctgaag gtgctcggca tcttcgcccg cctcaactac cgcgacggca agcccaagta
    30841 ccttgaggac acgccgcgct tcatccagta cgtgcgcaag acggcccgcc gctacatcca
    30901 actctcgccg ctcacgcacc tcatcgacga cctcgagggc tccgaaacgt ccttcggcta
    30961 caccttctga cggtcatgcg cgctctgatc ctcgcggcgg gggaaggccg ccgcatgcag
    31021 cccgtcacgc tcacgacgcc caagcccctc gtcgaggtgg cggggacgcc catggccgtt
    31081 cgccagattc ttgcactcag gaaggcgggc gtcgaggaga tcgtcctcaa cgtcgcgcat
    31141 ctcggcgaca ggatcgtgga gacgcttgga gacggctccg cctggggcgt gcgcctgcgc
    31201 tattcagtcg agggccgcac ggcgggcgaa gccctcgaga cgctcggcgg aatcgcgaag
    31261 gcgcttccga tgctcgatga cggcgacggc gcattcatcg tcgtcgcggg tgacatcgtg
    31321 accgacttcg actactcgcg ccttgtgcgt gcgggccgcg ctctcgcggg ctcggacgca
    31381 gttgcgcacc tcgtgcttgc gcccaatccc tcctaccatg ccgacggcga catgacgctg
    31441 gatgagctgg gactcgtccg aagggagccg aggacgcaca cgttctcgag cctcggcgtc
    31501 tacgggacgt cgctcttcga gggcgtcgag cctgttcgtg caaagctctt cccgtggctc
    31561 tggcagttct gcgaggcggg acgcgtcacg ggcgaggtgt acgagggcat gtggttcaac
    31621 gtgggcgacg tgcgcgagct cgcgcttgcc gaggcgcgaa ttcgaaacca aatggaaaag
    31681 tgagggcacg gcagggcctg tgcgccggtc gtgtcggtaa aatgtggcgg tcgggcgctc
    31741 cccgtgtgca gagcgccccc cgaataacgt gagaagagag tgaagctcgg tccctacgcc
    31801 gtcgatgttc ccgtcatgct ggcccccatg gccggcatga gcgatctgcc ttaccgcgag
    31861 atctgccgcg cccatggtgc gggatatgcg gtgggcgaaa tgacgacctg ccagccgcag
    31921 ttcaggacga gccgcaagag cgccacgcgc tgggcctgtg acgccgagtt gggactgcgc
    31981 gtcgtgcagc ttctcggagc tgatcccgcc atgatggccg acgccgcccg ctatgccgag
    32041 gattcgggcg cgcaggtggt cgatatcaac atgggctgtc ccgccaagaa ggtgcttcag
    32101 acggcgtgcg gcagcgcgct catgaaggac gaggcgttgg tggcgcggat tctcgaggcc
    32161 gtgtgtgcgg ccgtctccgt gcccgtgacg ctcaagatcc gcacgggctg ggacagggag
    32221 cacaggaacg cgctcgccat tgcgggcatc gccgagcaga cgggcgtcgc catgctgacg
    32281 atccacggca ggacgcgcga ggacggcttc aggggcgagg ccgagtacga gacgatcagg
    32341 accgtgaagg ccgccgtctc cattcccgtc atcgccaacg gcgacataga ctcgggcgag
    32401 aaggcaaggc gcgtgatggc gcttacgggc gccgacggcg tgatgatcgg cagggcctcc
    32461 tacggcaatc cgtggatctt ctcggaggtc ggcgccgccc tcggccatct tcccgaaagg
    32521 aaggcgccgg ggcttgagga acgacatgcg gtcatttgcg accacatgcg ccgtcatttt
    32581 gactattatg gcggtgctag aggtgccgtg accttcagga agcacctgag ccattatttg
    32641 aaggggctgc cgggctccga ggcgttgttg ggcgagttgt tcgccacgtc cgatgcggac
    32701 gtcctcggtg cggccgtcag ccgttatttt gagaggttgc aggatgacga gcagggactt
    32761 ggatgacaag gcgattgtcc gccgtaagcc tgaagcgccg cagatcaggc tgatcaggca
    32821 ggcgaagcag agagtcaagc tgggacttga cttcgagggc gttacgctgg aaagcctggc
    32881 gaaggacatg cccgagctca aggcgggccg cattgaagag gccgtgatcg tccagctcaa
    32941 ccagtacttc cagcagctcg gcggttcgca gccgcatcct ctctacagtc tggtcatgga
    33001 gacggcggag cgtccgttgc tcatctacgt gatgaagctc tgccgcaaca accagattga
    33061 agccgcaaag attcttggca ttaaccgaaa tactctgcgc tcgaagcttc gcagtcacaa
    33121 gcttctgccc ggagaaagtt cataaggaaa taggcaatga agaaacaggc tcttatcagc
    33181 gtgtccgaca agaccggcgt cgtggatttt gcccgcgagc tcgtcgctct cggctaccac
    33241 attctcagca cgggcggcac cgccaagctc ctcacgaagg aaggcgtccc ctgccaggaa
    33301 gtcgccgact acacgggctt ccctgaaatg ctcgacggcc gcgtcaagac gctcaacccg
    33361 aagatccacg ccggcatcct cgcccgtcgt cccgtcgccg agcacatggc cgctctcgag
    33421 gcccacggca tcgacccgat cgacatcatc tgcgtgaacc tctatccgtt cgaacagacg
    33481 atcgccaagc cggactgcac gtttgaagac gccatcgaga acatcgacat cggcggcccg
    33541 acgatgatcc gcgccgccgc caagaaccac gagtccgtcg ccgtgatcgt tgatccgacc
    33601 gactacgcct ccgtcgtcgc cgaaatcaag gccgaaggcg gtgtcacggc cgcgacgcgc
    33661 ctcgcgctcg ccaagaaggt cttcgcccat accgcccgct acgacggcat gatcacgaac
    33721 tacctcacga gccttgacga aaacaaccag cgcacccgct tcccgcaggt cctcacccgc
    33781 cagtgggaaa aggtccagga catgcgctac ggcgaaaatc cgcaccaggc tgccgccttc
    33841 taccgcgaat cctccgttgc gccgggcctt ctcgccggct atgaacagct tcagggcaag
    33901 gaactctcct acaacaacat cgccgacagc gatgccgctt gggaatgcgt ccgctccttc
    33961 gaggctcccg cctgcgtgat catcaagcac gccaatccgt gcggcgtcgc cgtgggcatc
    34021 agctccgccg aagcctactc caaggccttc cagacggact ccaagtccgc cttcggcggc
    34081 atcatcgcct tcaaccgcga agtgaccggc gaatgcgccg agctcgtttc ccgccagttc
    34141 gccgagtgca tcatcgctcc gagcttctcc aaggaagctg tcgagatctt cgccgccaag
    34201 aagaatctcc gtcttctcac ggtcgctctc ggcaaggccc acaacgactt cgacttcaag
    34261 cgcgtcggcg gcggcctgct cgttcagacg cccgacaacc agatctgcgc cgaaggcgcc
    34321 ctgcgctgcg tgacgaagcg ccagccaacg tcgtcccaga tcgccgacct gatgttcgcc
    34381 tggaacgtcg cccgcttcgt gaagtccaac acgatcgtct atgcccgcgg cggcatgacg
    34441 ctcggcgtcg gtgccggcca gatgagccgc gtcgactccg cccgcatcgc catgctcaag
    34501 gctgaagagg ccggtctctc tctcgagggc tccgtggccg cttccgacgc cttcttcccg
    34561 ttccgcgacg gtctcgacgt cgtggtggac gccggctgca cggccgtcat ccagccgggc
    34621 ggctccatcc gtgacgacga agtgatcgcc gccgccgacg agcgcggcat cgccatggtc
    34681 ttcacgggcg aacgccactt ccgccactaa ggcggtcccg aggcgcgggg agccgtgagg
    34741 ctcctgcgcc tcaaacgcag cggtgagcgg cgtttcgacg ccgcctccgc gtaccgaatg
    34801 caagccgctc cccgacggga gcggttccgt gcttacaagg gagtcatccg tgaccaactt
    34861 caatccttcc cgccgcagag tgctgtgcgc tggcgtgctt gcaggcgcct tcacggcatg
    34921 tcctgcgctt gccgaacttc aggtctccat cacgggcgtg ggcgccaatc agctcccgat
    34981 cgccgtcaag gtgtttgaag gctccggcgc cacggggttc gatgctgcgg ccgtcatcgg
    35041 agccgacctt gagcgatccg gcgcgttccg cctgatcccg acgggcgccg aaggcgaacc
    35101 cgagaacctt gaaaagcccg cgagcctcga ggccttcggc aagctcggcg cgaacgcgct
    35161 cgccgtgggc cgcgtcagct gtcttcccga cggccgctgg gagctcacct actatctgca
    35221 caacaccgtg acgggtgcgc tgacggactc ggccgtcttc acgacgtcgg gcaacgacgt
    35281 gcgcatgtgc gcgcaccgca tcgccgaccg catctacacg aatctcacgg gcgaaggccc
    35341 gatctttgcg tcgaagctcg tctacgtggc ccagctcgcc cgcaaccgct atgagctcgt
    35401 catcagcgac agcgacggcg gcaaccgcca ggccgccctt cagtcccatg aaccgatcat
    35461 ctcccccgtc tggtctcccg acggcaagaa aatctcctac gtgagcttcg aggaccgcaa
    35521 gccgatcatc tatgttcagg agctcgcgac gggcgcccgt catgcgatct gcgccttccg
    35581 cggcaacaac tccgcgccgg ccttcagcgc cgacggcggc acgcttgccg tggcgctctc
    35641 ccgcgacggc ctcacgcaga tctacctgat gaacgccaac ggcaccggcc tgcgccgctt
    35701 tacgaagagc tacggcatcg acaccgagcc gtgcttctcg gcggacggcc agtgggtcta
    35761 cttcaccagc gatcgaggcg gcgctccgca gatctatcgc cagccgctcg ccggcggtcc
    35821 cgccgagcgc gtgaccttcg gctctagcta cgcgatcagc ccgacggtca gccgcgacgg
    35881 ccgctatctc tcctacatct cccgcatcga cggccgcttc cgtacggccg tgatggatct
    35941 cacgacaggc cagaacacgc tcgtgaccac gaccgaccgc gatgaatcgc cgtccttcgc
    36001 gcccaacggc cgcttcctcg tctatgcgac ggaagtcaac ggccgcggcg ttcttggaac
    36061 ggcctccgtc gacggcaggc tctcgacgcg cctcaccggt ctgggcgaca tccgtgaacc
    36121 ctgctgggga ccgattctgc cctgatgaag ggcgaaaacc aacgacaatc aaccacaaca
    36181 ctgttgccag gaacacaaaa tgtccttttc gtggaaaatc ctcgctgcct ccgcagccgt
    36241 tgccgctctt ctgagcggct gctcgtccgt ctcccttgac gaaagcgcca aggacggcac
    36301 gctcgccggc cagaccgccg gcagccagac ccaggcggtg aatccgttcg ccgatccgaa
    36361 caacccgctc tccaagcgct ccgtgtactt ctccttcgac tcctatgaag tcgaagccca
    36421 gtacatgccc gccgtcgaag cccacgcccg ctggcttgcc gctcatccga acgtcaacat
    36481 catcatccag ggcaacaccg acgaacgcgg cggccgcgag tacaacctcg ccctcggcca
    36541 gaagcgttcc gaagccgtca agcagcgcct gcagctcctc ggcatccccg ccgagcgcat
    36601 cgaagccgtt tccttcggcc gtgaaaagcc ggtggcctac ggtcacagcg aggaagcctg
    36661 ggcccagaac cgccgcgccg acatcgtcta tccgacggaa gtgacgtccg gcaaggccgc
    36721 ccagtaatct ctggacgcat tacctgagac gacggccgga cgcagcccca cggggaccgc
    36781 ggccggccgc ctgcttttcg agcccggctc gtgcacggac acgagcggct tcttcggaga
    36841 aattatgaat ttgaacaagc tgatggttca ggcctgcacg gccgcgatcc tgtcgggctc
    36901 cgccgcgggt gcgatggcct tcgcggacga cgatgctcgc cgcgccattc ttgatctgcg
    36961 cgcacaggtt gccgcccttg aggaacagct caagaacgca cagatttcgt ttgcgacgcg
    37021 tctcgacaac ctgcagaacc agaaccgcct tctcaccggt caggtcgagg agctcacgaa
    37081 tgcgatccgt caggaaaagc gcagcacgcg cgagctctac accagcatcg acgagcgcct
    37141 cggcaagttc gagccgcgcg aagttgaggt caacggcgag aaggtcatgg ttcagcctca
    37201 ggagcaggcc gcctacgacg ccgccgtcga gcttctcaag gcgggcgact acaagaaggc
    37261 cgccgccgca ttcagcactt tcggcgccga gtggcccaag tccgcctacg cggccgacgc
    37321 cgtctactgg cgcggatcct gcctcttcgc ccttgagcag tacaagagca cgatcaacgt
    37381 tcagaattcg ctcatccgca gctacccgaa gcatccgcgc gtggccgatg ccatgatctc
    37441 cgtggcgtcg tcccaggcgg cgctcggaaa cgtcaaggca gcctctgcga cgctcgccaa
    37501 ggtgatcaag cagtatccga attcggacgc ggccaaaacc gccgcccaac ttcagaaaac
    37561 tttgaaataa gtcgacgaga gcactcaaat gcagcctgtc ctgtcatagg ggtaggctat
    37621 tattttgtcg ggtgaagagg ggaaaatgcc gtggatagcc gcaaaatatg agcagaagcg
    37681 gaatttttag gttgtttcac ttaccgaagc cgtttctact ggcctcacag agttgctaga
    37741 atcaccatcg gaggtgtcgc catggatttg gcggcacctt cagtacctta taactgcccg
    37801 gacaatttcg taccccgtgg agttttttag caagaaggac ttatgactga atccaagcaa
    37861 atttgcggcg ccgacctgct ccgcaacccg cagctcaacc gtgccgacgc cttcactcag
    37921 gaggagcgcg acgcccgcgg ccttcgcggt cttctgccgc cggccgtttc caccatggag
    37981 ctgcaggtca agcgcacgct cgctttgctc gaccgctgcc cgacggctct cgacaagttt
    38041 ctgatgctcg acagcctgca tgcgactgac gaagacctct acttcaagat cctgatcgac
    38101 tacatcgacg actacatgcc cgtggtctac accccgaccg tcggcgaggt ctgccagaag
    38161 ttcagccata tctaccgcta tccgcgcggc gccttcatct cgatcaacga caagggccgc
    38221 gtccgcgaaa tcattgaaaa ctgcccgaac gaggaagtcg acgtcatcgt cgtgaccgac
    38281 ggccagcgca ttctcggcct cggcgacctc ggtatcaacg gcatgggcat cccctgcggc
    38341 aagctctcgc tctacacggc ctgcgccggc atcgccccgg aaaagacgct tcccgtcacc
    38401 atcgacgtcg gcacgaacaa cccggaatac ctgaacgatc cgatgtacct cggcctgcgt
    38461 caggaacgcg tgacgggcga agcctacgac gaactcatcg aggaattcgt caccgaagcc
    38521 cgccgccgct ggcccaacgt cctcatccag ttcgaggact tcggcaacaa ccacgccttc
    38581 gaccttctcg aacgctacaa ggacaaggtc ctctgcttca atgacgacat ccagggcacg
    38641 gctgccgtgg ccgtcacggg cgtctactcc gctcttcgcg tcacgaagca gaagcttgcc
    38701 gatcagcgct tcctcttcct cggcgccggc gaagccgcca ccggcatcgc cgaactcctc
    38761 gtcgacgcca tggtcgccga aggcgttgaa cgcgaggacg ccctcaagca ctgcttcctc
    38821 ttcgactcca aggggctcgt gacctgcaac cgcggcgaca agctcgccca caacaaggtt
    38881 ccgttcgccc acgacgtcga gaactgcaag accttcatcg aagccgtccg cgccctgcgt
    38941 ccgaccggca tcatcggcgt ggccgcccag ccgggcgcct tcaatgccga agtgctcggc
    39001 gccatggccg aaatcaacga gcgtccgatc gtcttcgcgc tttccaaccc gacgtcccgc
    39061 gccgaatgcg acgccgccac ggcctaccgt gaaacgaacg gccgcgcgct ctttgcctcc
    39121 ggctctccgt tcgctccggt cgacttcaac ggcaagacct tcgtgccgcg tcagggcaac
    39181 aacagctacg tgttccccgg cctcggcctc ggcgccgtct tcgcccgcgc caagacgatg
    39241 cccgtgggca tgttcctcgc cgcctctcgc gagctcgccg agctcgtgag cgaagccgac
    39301 ctcgaacagg gctcgctcta tccgccgctc tccaccgtgc gtgaagtctc ggtcaagatc
    39361 ggtgctgccg tcgcccagta cgcctacgac aacggtctcg ctggcaacga acgcccggcc
    39421 gatctcgaag ccgccgttcg cgaattcatg tacaagcccg aataagttcc ggcttcatcc
    39481 gtgaatgaat gagaaaagcc tccgcacttc ggtgcggagg cttttttgtc aggatttcgt
    39541 caggattcag tcaggagtcg caggcagccg cagggcggga cccgagggcg tgcgttgtgg
    39601 tattctcccc gacgcccgag aggtcagccc tgatgcggcg ggccttcatc ttctgcagta
    39661 acccgtcc
//
LOCUS       DBMDTI010000003        36784 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_458_length_36784_cov_3.722078, whole genome shotgun sequence.
ACCESSION   DBMDTI010000003 DBMDTI010000000
VERSION     DBMDTI010000003.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 36784)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 36784)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36784
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_458_length_36784_cov_3.722078"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="ACZPB6_00475"
     CDS             complement(<1..766)
                     /locus_tag="ACZPB6_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1182986.1"
                     /translation="MRHTFSLVCAGLLALTLLAGCEKTTAPKTVRAPLSVETLTVEAA
                     DQPRWITLLGQAEGGREVEVKSQVSGILKKVAFTEGAEVKAGDVLYQIDPAPFEARLK
                     SAAANTRSAKATLAEAKTSYERTRALFEKGAKSRQDLDEANRAHLVASSAYNAATASE
                     RDAAISLEWTTVRAPASGLVSDTQFNEGSLISSASSVLATITQHDDVRVVFAPSARAL
                     DGADVTTDNAVKLRDERGRTLSGKLDYVAQSVNPKTS"
     gene            complement(861..2666)
                     /locus_tag="ACZPB6_00480"
     CDS             complement(861..2666)
                     /locus_tag="ACZPB6_00480"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIR1182987.1"
                     /translation="MNQSSAERLGKFRQTMKDRGLDAWIGRIVDPHLSEYVPEHWQSV
                     AWLTGFTGSTARLCVTQGAAALLVDSRYWEQAEGELDGAPFEVVRLGAPDAPSQEAWL
                     SQHLTRGRVGLAPELWSESDVRRIRTSLADCGLELALVPDLFDDVWTQGRPARPQTAV
                     TPMTQDSSTVADRLARVREVLRDRASEALCLHSLDDIAWLTGCRAADIKFNPVFLASM
                     IVTDSEALLYTEASRFTPELLGALAAQGIEVRAPESLAGDISALSRDLVFMADPDRLP
                     ASIFGLLERVPVEALSPVTLMKSRKSEEELEGIRRAMVSDGVALCEFYALLDERLAEG
                     AVLTESDVANMLDSERAARPDNRGLSFGTISAFGRNAALPHYAPKRETAAVLTDGLLL
                     IDSGGQYEAGTTDITRMTAVGRIDDDMKRDVTLVLKAHIALARAVVPAGVSGSQLDAI
                     ARAPLWAKGLDFGHGTGHGVGCRLNVHEGPFHISPRATKTGELGLQAGILVSDEPGLY
                     RPGHRGVRIENLIVARRAASTEFGEFLAFDVATLCPIDLRTVDVSLLTPDEVAWLDDY
                     HGKVLTALQNEALSTRAQAWLKARTVPAFRSRNIV"
     gene            complement(2720..3106)
                     /locus_tag="ACZPB6_00485"
     CDS             complement(2720..3106)
                     /locus_tag="ACZPB6_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1182988.1"
                     /translation="MSASLPVRSLLAATAAMLLLSACSTAYYTGDTISGPAQRMKRDA
                     DGNLHDPLLDAPNRSLMTCTSEAPVTVLQRVGEVPFACPDLGVSATLDELRDAGWRIL
                     RLDIGEDLESDSHVGFPVTVQVRKLF"
     gene            complement(3254..>3971)
                     /locus_tag="ACZPB6_00490"
     CDS             complement(3254..>3971)
                     /locus_tag="ACZPB6_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1182989.1"
                     /translation="DAVMQPQAAPAEGAASEQAQTRKPRKSFTERRFGKNRPFKQRQE
                     DAAETSSAPVEAQAVPAAEAGAQDKSRKDHFKNFRRKSRKFQNGEGEASRTQEKPVQN
                     ADSEQAAAGDRKPQEKKTFEKKAFEKKPYEKKPFEKKRDRKKPARQDAEAKPRQQDWD
                     DDNFGNSIHYQPKRQNLRGLPSDQQIHWEPTDPYHPSSQALSLPQIMPDENRRGGYVN
                     GNVKGGFNRSRRNYRKKREG"
     assembly_gap    3972..4071
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4072..5399)
                     /locus_tag="ACZPB6_00495"
     CDS             complement(<4072..5399)
                     /locus_tag="ACZPB6_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR1182990.1"
                     /translation="MLSWLPEAFKSWATSNPFLSSTLNEVLNERDTRLSGTPCTSRLK
                     TAVTALLQKLQDRRDKSAALILAASREAEETAFTLASRMSEGTGLRIAATTGVRDGAG
                     LAAIFATAPDLVVTNPKVLKKVFDKGFVAPEAFRTILVDGAEWHDVMGDTDLIASLVE
                     RFPAALQLIVTGPVVVETTEDPYNAMLHAPAYCRAPDEAARETAPKERVVLVPEEKMS
                     ETLAREAEKTPVLFLVSTPTESTRVTGLLKEAGIAAKRATATQSASARETLVLKFIAG
                     RVEHLVMPHSVIGELEHNRASRVILTDLSGGPEPYLEHLALTADGTGELVTIVTPETL
                     PLFEKLLVAAEKRLTLENPYNLPEPRTVIGQLLRREKLTIRTKYGRTAESTEGDRGEQ
                     DDRGEGRSRWERRKSKNGQRKDRKDGFRKDRKDFRKDEQGKTFGKKPRKAA"
     gene            5809..6147
                     /locus_tag="ACZPB6_00500"
     CDS             5809..6147
                     /locus_tag="ACZPB6_00500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR1182991.1"
                     /translation="MQEELKDLKIEELAEGTGRQAMKGDTIAAHYTGWLEDGTKFDSS
                     LDRGEPLEFVCGVGMVIKGWDMGVVGMREGQKRRLTIPAHLGYGAYGVPGCIPPNATL
                     IFEVELVKVY"
     gene            complement(6230..6862)
                     /locus_tag="ACZPB6_00505"
     CDS             complement(6230..6862)
                     /locus_tag="ACZPB6_00505"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR1182992.1"
                     /translation="MSSKTIFLASKSPRRRDLLTRLGYDVRVIASNAHTLMAFEGDEV
                     VLPDEAPKAYVLRTARNKFLEGLGVKNAGEFAGVRLPIVAADTVVSLGNEILGKPRDF
                     DEACLFLEKLSGRTHDVRTAVYVGLSETACDWRISHSTVSFRELTHDEIEAYANSGEP
                     YDKAGGYGIQGLASIFISNISGSYTGIMGLPVYETAELLANAGLPVFNAQ"
     gene            complement(6868..7335)
                     /gene="rlmH"
                     /locus_tag="ACZPB6_00510"
     CDS             complement(6868..7335)
                     /gene="rlmH"
                     /locus_tag="ACZPB6_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR1182993.1"
                     /translation="MQIRIVAVGQRITEWAQSAVSDYLSRFPRDFRVELREIRTEPRA
                     GQTPARLMAAEAERILAAIEPDDFVVVLDEHGKDLTTMDLARHFSHWRADGETPVFVI
                     GGPDGLAPEVKARANLTIRLSSMTLPHAFARVMLSEQIYRAWSILAGHPYHRA"
     gene            complement(7339..7743)
                     /gene="rsfS"
                     /locus_tag="ACZPB6_00515"
     CDS             complement(7339..7743)
                     /gene="rsfS"
                     /locus_tag="ACZPB6_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR1182994.1"
                     /translation="MNIEELTQLVVNALEDVKAKDIKVFNTEGLTDQFERVVIASGTS
                     NRQTRALAYSVSHEVKQAGGDVLGMEGQETGEWVLVDLGPVVCHCLQPNVRDYYNLEE
                     IWGGKEVFLKANEECASRLMPHRPGHMPEDFE"
     gene            complement(7757..8449)
                     /gene="nadD"
                     /locus_tag="ACZPB6_00520"
     CDS             complement(7757..8449)
                     /gene="nadD"
                     /locus_tag="ACZPB6_00520"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HIR1182995.1"
                     /translation="MTDSLALYSARRGLGILGGTFDPVHAGHLCLARAALDAVPLVRV
                     ELMPAGEPWQKSRISPGIHRLNMLRIAVEYEPQCRINTSELCRSGATYTIDTVSRLRE
                     EVGPDMPLVLILGSDQWFNLSTWREWQSLTDFVHIAYCARDGVRESEIPESQALWAAP
                     RMTEARKLVFSPSGRIARFEMAPHRASATAIRRTLAREPFARAMSSLDGWLDPGVASY
                     IRTHGLYQPARH"
     gene            complement(8458..9111)
                     /locus_tag="ACZPB6_00525"
     CDS             complement(8458..9111)
                     /locus_tag="ACZPB6_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1182996.1"
                     /translation="MHPAFLSKLPALLAGALLPLALAGGAHAEKITGPFAYPTGLLDA
                     PATLTTPLGRDARIGKPRIVLESTRLEDLAAMTGATRLSEGDGVFRRDTLCLTGTTHG
                     KPMIVWFIATDSDFVTEAQLEWAGERTVPEICRRLDAEHLPVQIGRIGLGMESALVNE
                     LLGRASYKDEAGWHYWFSQRFLRNKRGLQELELNWLAVHYDDDGLVDKTFSSQVTNL"
     gene            complement(9318..10601)
                     /gene="purD"
                     /locus_tag="ACZPB6_00530"
     CDS             complement(9318..10601)
                     /gene="purD"
                     /locus_tag="ACZPB6_00530"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR1182997.1"
                     /translation="MNLLVVGNGGREHALAWRLAQSDRVTKVFVAPGNAGTALTPRLE
                     NLPITDIDQLIEFVKSNNIAFTVVGPEAPLAAGIVDKFRAAGLRIFGPTKAAAQLESS
                     KDFAKAFMKRHNIPTADYESFTDPEAAHAYVRRKGAPIVVKADGLAAGKGVVVAMSEQ
                     EAHDAIDMMLEGHTFGNAGARVVIEDFLDGEEASFIVMVDGENILPMATSQDHKRLLD
                     ADQGPNTGGMGAYSPAPVVTPDIHAQVMREIIRPTVQGMAADGIRYTGFLYAGLMISH
                     GADGKPIVRTLEFNCRFGDPETQPIMMRVKSDFSEIIEAGIDGKLDSVEVEWDRRTAL
                     GVVCAARGYPAHPEKGAVIEELPENTAEQMVFHAGTALNEEGLTVVSGGRVLCVVGLG
                     ESIAAAQKSAYAAARQVKFDGMQMRSDIGYRALNR"
     gene            complement(10820..11554)
                     /locus_tag="ACZPB6_00535"
     CDS             complement(10820..11554)
                     /locus_tag="ACZPB6_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1182998.1"
                     /translation="MAGHSKWANIQHRKGRQDAKRSNLWTKLVREIIVAARMGGGDPE
                     SNSRLRLALIKARGGNVPKDTISNAILKGTGQLEGVSYEEIRYEGYGIGGAALIIDCT
                     TDNRVRTVADVRHALTKHGGNLGTEGSVSFMFKHIGEFVFAPGVTTEDAVMEIALEAG
                     ADDVVSDEDGSIEVTCEPAAFDAVSKAFEAAGIEPVVSEITYKALNEVKLTGEDAEKM
                     QKLIDALEDLDDVQQVYTSADFDDEE"
     gene            complement(11778..13055)
                     /locus_tag="ACZPB6_00540"
     CDS             complement(11778..13055)
                     /locus_tag="ACZPB6_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1182999.1"
                     /translation="MTCTCAYHDQLIEIRRHLHTMPEEGWSEFTTTAFIVGKLREYGY
                     EVLLGKKVINPDNCLGRNLKVVEAGLKLARENGVSEELLAEMDGLTGCVGVMDTGRPG
                     PTLAIRFDIDCVPVTESTEDCHIPAREGFISKRPGLMHACGHDAHTSTGLAVAHWFAD
                     NVDQMNGKIKILFQPAEEGVRGAAGMAASGILDDADYFLGAHIAMMCKSGEVSVNPYG
                     FLCTTKLDVTYTGRPAHAGVEPNAGHNAMAAACNAFVQLLGIARHGSGMTRINVGQLV
                     AGEGRNVIPSHAVMKMEVRGETGEINQYMYDSAVNIIKGCALSQNCEYTIEKMGEAVD
                     LTNDPELVKVLTEAVGNVEGMTARQDPMNFGGSEDATILARRVQAHGGKAAFFVLGAD
                     RPSGHHTAKFDIDEKALDKGLAVWANAIKLILK"
     gene            13452..14720
                     /locus_tag="ACZPB6_00545"
     CDS             13452..14720
                     /locus_tag="ACZPB6_00545"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HIR1183000.1"
                     /translation="MSVTGLQTEGLKYAKAIFDTVRRMSADTLGVSRQGYSELETDVL
                     HYLQGLGRDIGLEVRADDAGNVWMTMPGRDRTLPAFVAGSHVDSVPQGGNYDGLAGVT
                     AALTVAWWMHRHQVQLERDYTVLMMRCEESSYFGKAYVGSLGMMGRLTQSDLMLRHRT
                     EDITLGQCISACGLDPSKLTAGTPIVDLKKIACFVELHIEQGPTLTSNDKVRVGVVTG
                     IRGNVRHKNVVCHGETAHSGAVNKEFRHDAVMATAALLSRMDNHWQEWLDRGEDLVFT
                     VGVIRTGATAAISVIPGEVSFTLDMRSLSADTCERFHQLLLEEAAALEKERGVRFDFD
                     KPLYTAPGLVDQALSDRIYKAAEDNGIPCMRLPSGAGHDAAVIGAAGIPVGMIFIANQ
                     NGSHNPHEEMIMKDFMTGTDLLWQTVRQFD"
     gene            14732..15409
                     /locus_tag="ACZPB6_00550"
     CDS             14732..15409
                     /locus_tag="ACZPB6_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR1183001.1"
                     /translation="MDRSKLISVVLVDEYPLFRKAMADLLNATGEFQVLGQTGEGDTA
                     LGLSSLGPDIVVMSLEAEEYSALDLLKEIKYRQPATRVVMVMSSADSSGQLMRAIRLE
                     ANGYLLRTITPSDFVEQLRKTAQGGMAVSEKVTSALAEKLRGDSYAVEEVRRVSELTS
                     REQDVLGYVAAGISNLEISKCLGISEGTVKVHVKHTLKKLKFRSRVELAVWASEHGFK
                     VKTAGRF"
     gene            complement(15767..17116)
                     /locus_tag="ACZPB6_00555"
     CDS             complement(15767..17116)
                     /locus_tag="ACZPB6_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HIR1183002.1"
                     /translation="MLGFDFWAQLVIVLVALFYGARKGGMALGLIGGIGLIILVFGFH
                     LKPGKPPVDVILTILSVICASAALQAAGGLDCLLQIAEKMLRKHPRAVCFLAPYLCWF
                     LALLCGTGHVVYTMLPIIYDVAIKNGIRPERPMAASTIASQMSIICSPAAVAAVSMIA
                     LCDGYQVGGKAFGFIQLFSITIPGALVGLFAISVFSMFRGKDLDKDEEFQKLIADPEQ
                     RKYVYGETTTLIGKTFRPEQWASLWIFLATVAAVAVFGMFPELRPIIGKKALSMTLII
                     QMFMLACGAFIVIFCKTRASSIGKTPVFSAGCVAITAVFGVAWMADTMFAAHLADLKA
                     VMTEIVRQYPWAYALVLILTSKLVNSQAAAVATIVPVALSIGVPPGLVVSCAAACYGY
                     YILPTYPSDLAALQFDRSGTTHIGRFVINHSFILPGLIGVLTATATGYVLGLIYGFI"
     gene            complement(17548..19197)
                     /locus_tag="ACZPB6_00560"
     CDS             complement(17548..19197)
                     /locus_tag="ACZPB6_00560"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR1183003.1"
                     /translation="MAQTPAFKYAPMFQTGEDKTEYYLLTKDHVSVGEFEGKKILKVE
                     AEGLTKLINRAFHDVNFMLRREHNEQVAKILSDPEATANDKYVALMFLRNAETAAKGI
                     LPFCQDTGTAIVHGEKGQQVWTGYCDEEAISKGVYKTYTEEALRQSQNAPLTMYEEVN
                     TKCNLPAQIDIEATEGAEYKFLCVVKGGGSANKSYLYQETKAVLNPAKLVPYLIEKMK
                     SLGTAACPPYHIAFVIGGTSAERTMLTVKLASCHYYDGLPTTGDETGRAFRDVELEQQ
                     LLEEAWKIGLGAQFGGKYMAHDIRVIRLPRHGASCPIGMGVSCSADRNIKCKINEEGI
                     WVEKLDDNPARLIPEELRKPGEAGGVTIDLNRPMKEVLADLTKYPVSTRLLLNGTIIV
                     ARDIAHARIKERLDRGEGLPDYIKNHPVLYAGPAKTPEGYACGSMGPTTANRMDPYVD
                     PFQAAGGSMVMIAKGNRTQVVTDACKKHGGFYLGTIGGVAAVLSSDCIKSIECIEYPE
                     LGMEAVYQIRVENFPAFILVDDKGNDFFQQLKPVTIACKKA"
     gene            complement(19439..20116)
                     /locus_tag="ACZPB6_00565"
     CDS             complement(19439..20116)
                     /locus_tag="ACZPB6_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1183004.1"
                     /translation="MADTVIKHPQAMLQLRLSNFSVAYQGIPAVVNLSLTLTAATVNL
                     VTGVIGAGKTTLLKALSGMIGHTGSIELTEGARRFGADGAGALLQPLSRSLISPYMTV
                     TQFLQIGLMQNTWQLPPDAGERVTDALARCRLEPLAHSPISVLTPEQYRLAEVAQLFV
                     QQPRVILLDEPSRCLSAESATSVLTCISKWVRDTRNIAVVAAEGVIPAANVDRILTLE
                     RGKLMNA"
     gene            complement(20165..20950)
                     /locus_tag="ACZPB6_00570"
     CDS             complement(20165..20950)
                     /locus_tag="ACZPB6_00570"
                     /inference="COORDINATES: protein motif:HMM:NF019322.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1183005.1"
                     /translation="MTTIKSTRTPLWSRLAQTLAGDILSGRLPVGSLLPTEAQLMNDY
                     GMSRHTVRSALAELVHLGLIRRSPRRGTIVTGDGRGAEFTASLVPSLPADAGGRTLLS
                     IRRMICDEDTAARTGFALLTPLITLTMLTKGPDGRAVARTELWVRDAARDLIPYLTPQ
                     SPRSVLALLEEHAGVRCRSMHQSAEAAALDEACSKLFGVPEGSPALVVTRTYLDRRPV
                     PLIHAVETRPAGVRACETVFLRSTAARTAEHEGSPASEPIPTD"
     gene            complement(21059..21886)
                     /locus_tag="ACZPB6_00575"
     CDS             complement(21059..21886)
                     /locus_tag="ACZPB6_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     transmembrane subunit"
                     /protein_id="HIR1183006.1"
                     /translation="MSTPDSVKYSNTPTATENVWGAGLKDAKRQSPSTALADVAQSVT
                     GVILGIFLFCHMAFTSSVQISKDLFANLINTSGGMFMFAEEQAWLHVVFVGFITLCVV
                     IHAFCALRRFPTSYHQLRDIKAHYKMLRHEDTTLWMVQLVTAFLLFIFVFPHLISMLC
                     NPHGFDVNLIGVHTHHMGMIYTFVFLVITELHGMIGLYRLAVKWDIFAKNPETDIIDQ
                     RNGDRAGLRKTFLFIALLMIVCGTLTAWKNYSIGAELIENGQEATRYVLADENNWWK"
     gene            complement(22252..22899)
                     /gene="upp"
                     /locus_tag="ACZPB6_00580"
     CDS             complement(22252..22899)
                     /gene="upp"
                     /locus_tag="ACZPB6_00580"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR1183007.1"
                     /translation="MRTDSRFPRLHIVDHPLIEHKLSLMRDKNTGTQDFRTLLHEIAL
                     LMGYEVTRDFPTKLEDIETPICATKSPMLVEKDPVIVPVLRAGLGMTDGLLALLPAAR
                     VGHIGVYRNAQHEPVEYLVRLPDAPNSNYIVVDPMIATGHSAAYAIEILKRRGVRGEQ
                     IHFMALVAAPEGIKVLSEAHPDIDIFVAALDEKLNDRAYIVPGLGDAGDRIFGTL"
     gene            23249..23866
                     /locus_tag="ACZPB6_00585"
     CDS             23249..23866
                     /locus_tag="ACZPB6_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR1183008.1"
                     /translation="MKIRTIFLLFVLILMAVFLVINWTALSTVTAVNLIYTEIQAPLG
                     VLVVGGFAAVVFVLLVYMVWQQASVTLELRAAYKEARNARTVADDAEKSRFAESNRIL
                     LERIEKLEALMTARSDETLTAVRQEAVRAEERYKALFDDQKRAQGELTAQVQTQIGAM
                     EKRVVETLPSAVSAAVPTQALAEEAEKKADAEESCARKSVFKDLF"
     gene            complement(23964..24962)
                     /locus_tag="ACZPB6_00590"
     CDS             complement(23964..24962)
                     /locus_tag="ACZPB6_00590"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HIR1183009.1"
                     /translation="MSTLSLSQYLCREQQLHDLDPVLSRLIVSVSEACKTISFKVRNG
                     ALAGVLGMAGSENVQGEDQKKLDIISNDIFVEAVALSGSVCGMVSEEIPEVIPVPEDL
                     PIGPYLICFDPLDGSSNIDINVSIGSIFSVLPAVRPKREPVAEDFLMPGTKQVAAGYC
                     MYGPQTQLVLTFGRGVVMFTLDPTTGTFMLTAEDVRVDRSAKEFAINCSNMRHWEDPV
                     KRYVAELLEGKTGVRGKDFNMRWVAAMVAEVHRILQRGGIFMYPRDHRDLTKPGKLRL
                     MYEANPMSMLMENAGAAATNGHQRILDIVPEKIHQRVAVFLGAADEVERVTSYHAE"
     gene            complement(24977..27643)
                     /gene="pepN"
                     /locus_tag="ACZPB6_00595"
     CDS             complement(24977..27643)
                     /gene="pepN"
                     /locus_tag="ACZPB6_00595"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HIR1183010.1"
                     /translation="MAETIFRADYRAPSHLIDTVHLEFDLDADSTSVINTMHVRPNPD
                     AQHSSDLILNGETLTLVSVAVDGLPLDESRYTLGDDLLTIRGIDRECDVIVVNRFSPS
                     ANKALSGIYTSGQNLMSQCEAQGFRRITYFLDRPDVLSRYSVVIRASKEQYPVLLSNG
                     NRIEEKDLDDGRHEARWEDPFPKPCYLFALVAGRLEALRDTIVLKDGRTASLEVWVEP
                     QDIDKTQHTMDSLKKSIRWDEERWGLELDLDGFRIVATNDFNFGAMENKGLNIFNARY
                     ALANPKVATDQDYANIESVVGHEYFHNWTGDRVTLRDWFQLTLKEGLTVFRDQEFSAD
                     MLGDESARAVERIRNVKALRLAQFAEDAGPMAHPIRPDSYQEIDNFYTSTVYEKGAEV
                     IRMLQTLLGKDTFRKGFDLYIARNDGHAVTCEAFLEAMEEASGRDLQQFRRWYSQAGT
                     PRVAVSTQWNAEAGQLVMTVEQTTPPTPGQPAKEPMLMPFPVALLDAEGREMPVLLKG
                     ESEGSAQLTRMFELKDARQTWVFSGLREKPVPSLNRGFAAPVILDVSLDADALALLAR
                     ADSDPFNRWEAMNRLMMKAILEQVRAVLANDQAPVNATLLEAFGAIIDDVNLSPAFRA
                     VAMELPSERAVADAMPIIDPAAIHRAREIVREALGIRWHSKLVSVIDGCRLEGEYSPD
                     STSAGIRALKNTCYYYGVAAGNKTMVLRVRDQFNTANNLTDRLAALNMIAGSSTPTKN
                     DTIVSALKEWYSEPLLVNKWLTIQATAPCFAGEAPVVTRVSELANSPFFSMANPNNVY
                     ALLVAFFMNNPAEFHRLDGEGYKFWVECVLKLDALNPQVASRVARSLENWRRYTPQLS
                     NLMFQALKHLERQEKLSVSVREIVEKALNNPA"
     gene            complement(27840..28445)
                     /locus_tag="ACZPB6_00600"
     CDS             complement(27840..28445)
                     /locus_tag="ACZPB6_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HIR1183011.1"
                     /translation="MANNSKWYVAGVLLIGAVIGIVFVGILTSVVHWAGSPKFCGEFC
                     HSMDLTYAAYKKGQHYMTSSGATAGCSDCHLLNHSNEHVGPLDYTALLFDKARAGSIS
                     LLGQIQGSLSTHEKQLEKRKEMAEAVHKQMIERNFSACRGCHDLSRMHNAKKPFIAKM
                     HQNLDKDPKKPADCLACHPKAGHDYAAADAEMAKAQETAAK"
     gene            complement(28677..29450)
                     /locus_tag="ACZPB6_00605"
     CDS             complement(28677..29450)
                     /locus_tag="ACZPB6_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR1183012.1"
                     /translation="MSKTIADPTLLATHMAELADKRIDILRRIGDVGSISEAARRAGV
                     SYKAAWQAIETLNNLAGAPLVEKVVGGNKGGGTRLTATGEEVLKLADELTRARAEVLV
                     RFRLKDSPALKNVGVSSLRTSMRNQFPAVIEKLKIGSAQVRLILSIDDTHYIRASCTK
                     ESAQLLGLREGKHVLALCKATAVDISADNPALVESRDNQIVGAVLRSEREDKGGECTI
                     QLPSGLTIVGFARPSHGLHIGMRAVATVAPEAVVIALNS"
     gene            29581..30336
                     /gene="modA"
                     /locus_tag="ACZPB6_00610"
     CDS             29581..30336
                     /gene="modA"
                     /locus_tag="ACZPB6_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1183013.1"
                     /translation="MQMKKLAAALMLAGLACAVAAEEVRVAVAANFMAPMKELAPLYE
                     KATGDKLVVSYGATGAFYAQIKNGAPFDVLLAADAKTPAKAVKEGHGVAGTTFTYAVG
                     KLVLWSSDAALVKDQKALLHPAVKKVAVADARLAPYGEAALQALDALKMKEAVEPKFV
                     VSNNIGKTFQFVESGNTQIGFVALSQCFKNGAFTGGSGWVVPQDLYKPILQDAVQLKA
                     GEKNAGAKRFLDYLKTSNEAAAIREAYGYGTVR"
     gene            30340..31017
                     /gene="modB"
                     /locus_tag="ACZPB6_00615"
     CDS             30340..31017
                     /gene="modB"
                     /locus_tag="ACZPB6_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR1183014.1"
                     /translation="MLTWTELGPVWLSLRLAAVTTVILLIIGLPLAWWLVKGRSALCA
                     PMNAVVTLPLVLPPSVLGFYILIALGPNGPIGQVTDALGLGTLNFTFTGLVIGSVVYS
                     LPFMIQPVVTAFEAIGPRPMEVAATLGCRPFEAFFRVILPLARHGIITGTLMTFAHTI
                     GEFGVVLMIGGNIPGSTQVISTEIYTYVEAMEYGKAHVLAGGMLVFSFFVLLSLNLLN
                     RKEGRTR"
     gene            31014..32087
                     /gene="modC"
                     /locus_tag="ACZPB6_00620"
     CDS             31014..32087
                     /gene="modC"
                     /locus_tag="ACZPB6_00620"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HIR1183015.1"
                     /translation="MSGRITLNLDRGTDFRLDVSFEIPDKGITVLFGPSGCGKTTVLR
                     CTAGLERAGGRVVVADRVWQDDARNIFVPAWERRLGYVFQEASLFEHLNVEGNLHFGL
                     RHKDARGGLARFSEAVELLGIGHLLRRRVQLLSGGERQRVAIARALLMTPDLILMDEP
                     LAALDWNRKQEILPWLERLRDELSVPMLYVTHSADEMARLADQVICFENGRIVAEGPL
                     EEVLLERWPGQGEQGSSVVLSGVVTERSEMWCTAVVTAGETAFEVPDAGRNVGEAARL
                     RILARDVSVALSEPRDTSIRNVLPAVVIGMRGVEGESHVMLTLEAGGCRILSRITRRS
                     ADELGIAVGTRLYAQVKAAAIIV"
     gene            32268..33176
                     /gene="argF"
                     /locus_tag="ACZPB6_00625"
     CDS             32268..33176
                     /gene="argF"
                     /locus_tag="ACZPB6_00625"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR1183016.1"
                     /translation="MPCHFLKLSDFSGETLRYMIDRAKVIKADLKAGKRYEPFAGKVL
                     IMVFEKASTRTRVSFETGFYQLGGNVVNLQSQGSQIGRSESIADTARVISRMGDLIMM
                     RTFGQDRLDDMAVHSRVPVINGLTNEYHPCQILTDILTFEEHCGPITGKTVAWVGDAN
                     NMAYTWIEAAKLLGFKLHFSSPKGYELDPALTLAGDHLKVFDDPVECCRGADLVTTDV
                     WTSMGYEEENAVRREAFAKWQVNADSMAAANQGAVFMHCLPAHRGEEVTADVIDGPQS
                     VVWDEAENRLHAQKAIMEYCLLGRVE"
     gene            complement(33359..34726)
                     /gene="purB"
                     /locus_tag="ACZPB6_00630"
     CDS             complement(33359..34726)
                     /gene="purB"
                     /locus_tag="ACZPB6_00630"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR1183017.1"
                     /translation="MELNPLTALSPIDGRYARQTDALRDIFSECAFMNARVRVEVEWL
                     ISLSEAGFAELPHLSDHGKAFLRGLADNFSLADCEAIKEIEKTTNHDVKAVEYWIKNQ
                     VAHDEELKSASEFVHFSCTSEDINNTSHAIMLKRGREALADYLQQIHDTIAGFAHQWA
                     EIPMLSRTHGQTASPTTVGKEFANVAVRLERVIEAIRAVKIYAKMNGAVGNYNAHLVS
                     YPEYDWEAHSRKVVEERLGVTFNTHTIQIEPHDYMAELFHQIERANTILIDFDRDVWG
                     YISMHFFKQKLREGEVGSSTMPHKVNPIDFENSEGNLGLANAVLDHLAGKLPISRWQR
                     DLTDSTVLRNLGVAFGYCFIGYNALTRGLGKLQVNEQVIAADLDNAWEVLAEAVQTVM
                     RRYGVPHPYEQLKALTRGKDGITKETMREFISNLDIPADAKASLMELTPATYIGKAVE
                     LARRC"
     regulatory      34953..35052
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            35136..36752
                     /gene="thiC"
                     /locus_tag="ACZPB6_00635"
     CDS             35136..36752
                     /gene="thiC"
                     /locus_tag="ACZPB6_00635"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR1183018.1"
                     /translation="MHNHFHSNPDLRPAGERFFVRSEAGEDVPLRRIRLSDTPECFGG
                     GRNPDFFMTDVTGGYGAADAGETVELVRRERPEPGSPTQLAAARAGVVTPEMAYAAAR
                     ENANLEGFAASLSEPGLERVRDRLMPLLERVEPWTGERVRARVAARQAVIPAAFTHPE
                     VEPTVIGRHFATKVNANVGTSASSSDWREEGRKMQEALARGADTVMDLSTGRCIRETR
                     EMILRGSPVPVGTVPLYETLERAGGDPLALSWEIFRDVLEDQARAGVDYMTIHAGLLL
                     PHVELTRSRLTGIVSRGGGMMARWMKAFGRENFLYEHFDEILDIAAHYDLTLSLGDGL
                     RSGSLYDGNDAAQFAELRTLGELNRRAGARDVQVMIEGPGHIPMPGVELNQLNEREWC
                     DDAPFYTLGPLVSDVGAGYDHITAAIGGAMIAGAGTAMLCYVTPKEHLGLPDANDVRE
                     GMAAFRIAAHAADIARGVPGAILRDHLMSMARFEFRWPDQFALAVDPARARAFHDETL
                     PGAAAQKAHFCSMCGPKFCPMKLAHELFKN"
BASE COUNT         6390 a        10376 c        12817 g         7101 t
ORIGIN      
        1 tgctcgtctt cgggttgacg gactgggcga cgtagtcgag tttgcccgag agcgtgcggc
       61 cgcgctcgtc gcggagtttg accgcattgt cggtcgtcac gtcggcgccg tcgagcgcac
      121 gtgcggacgg cgcgaagacg accctgacgt cgtcgtgctg ggtgatcgtg gcaagcacgg
      181 agcttgccga ggagatgagc gagccttcat tgaactgcgt gtcgctcacg agaccggagg
      241 cgggagccct cacggttgtc cattcgaggc tgatggccgc gtccctttcg gaggcggtcg
      301 ccgcgttgta ggctgaggag gcgacgaggt gcgcgcggtt ggcctcgtcg aggtcctgac
      361 ggctcttggc gcccttttca aaaagcgcac gggtgcgttc gtagctggtt ttagcttctg
      421 caagcgtggc cttcgcgctg cgcgtgttcg ccgcggccga cttcagcctg gcttcgaagg
      481 gcgcgggatc gatctggtag agcacgtcgc ccgccttcac ttcggcgcct tccgtgaagg
      541 cgaccttctt gaggatgccg ctcacctggg acttgacctc gacttcgcgg ccgccctcgg
      601 cctggccgag cagcgtgatc cagcggggct ggtctgccgc ttcaacggtc agggtttcaa
      661 cagaaagggg cgcgcggacg gtctttgggg ccgtcgtctt ttcgcagccg gcgagaagcg
      721 tgagcgccag aaggccggcg cagacaagac tgaatgtatg tcgcatgtcg ttgagggtgg
      781 attgttcctt gcttgcgttt cagtatggcc gcgaagccga agagaggcaa atgtcttcgg
      841 ctaacccggg ttaagcagtg ttacacaatg tttctggaac ggaaggcggg gaccgtgcgg
      901 gccttgagcc atgcctgtgc gcgagtagaa agcgcctcgt tttgaagggc ggtcaggact
      961 ttcccatggt aatcgtcgag ccaggccact tcgtcgggcg tgaggagcga aacatcgacg
     1021 gttctgaggt cgatcgggca gagcgttgcg acgtcgaacg cgaggaattc gccgaactcc
     1081 gtggacgcag ccctgcgggc gacgatcagg ttttcgatgc gcacgcccct gtggccggga
     1141 cggtagaggc cgggttcatc ggagacgagg atgcctgcct ggagaccgag ctcgcccgtc
     1201 ttcgttgcac gcggggagat gtggaaggga ccttcgtgga cgttgaggcg gcagcccacg
     1261 ccgtggcccg tgccgtgtcc gaagtcgagg cccttggccc agaggggcgc gcgtgcgatg
     1321 gcgtcgagct gcgagcccga gacgcctgcg ggaacgacgg cgcgtgcaag cgcgatgtgg
     1381 gccttgagca cgagcgtgac gtcgcgcttc atgtcgtcgt cgatgcggcc gacggccgtc
     1441 atgcgggtga tgtcggtcgt gcccgcctcg tactgcccgc ccgagtcgat gagaagaagc
     1501 ccgtccgtga ggactgcggc cgtttcgcgc ttcggcgcat agtgtggaag cgcggcgttg
     1561 cggccgaagg cggagatcgt gccgaagctc aggcccctgt tgtcggggcg tgcggcgcgt
     1621 tctgagtcga gcatgttcgc gacgtcggat tcggtgagca cggcgccttc ggcgagtcgc
     1681 tcgtcaagca gcgcgtagaa ttcgcatagc gcgacgccgt cggagaccat ggcgcggcgg
     1741 atgccctcga gctcctcctc ggacttgcgg ctcttcatga gcgtgacggg cgagagcgcc
     1801 tcgacgggga cgcgctcgag gagtccgaag atcgaggcgg gaaggcggtc ggggtcggcc
     1861 atgaagacga ggtccctcga gagcgcggag atgtcccctg cgagcgattc gggtgctcga
     1921 acttcgatgc cctgtgcggc cagagcgcca aggagttcgg gcgtgaatct ggaggcttcc
     1981 gtatagagaa gggcctcgct gtcggtcacg atcatgctcg cgaggaagac gggattgaat
     2041 ttgatgtcgg ccgcacggca gcccgtgagc caggcgatgt cgtcgagcga gtgcaggcac
     2101 agggcttcgc tagcgcggtc gcgaagcact tcgcggactc gggcgagcct gtctgcaacg
     2161 gttgaggaat cctgcgtcat cggggtgacg gcggtctgcg gacgggcggg acggccttgg
     2221 gtccagacgt cgtcgaagag atcggggacg agcgcgagct caagtccgca gtcggcgagt
     2281 gatgtgcgga tgcggcgcac atccgattcg ctccagagtt cgggggcgag gccgacgcgg
     2341 ccgcgggtga gatgctgcga aagccaggcc tcctgcgagg gggcgtcggg cgcaccgagc
     2401 ctcacgactt cgaagggcgc tccgtcgagc tcgccttcgg cctgctccca gtagcggctg
     2461 tcgacgagaa gcgcggcagc gccctgcgtc acgcacaggc gggcggtgct gcccgtgaag
     2521 cccgtgagcc aggcaacgga ctgccagtgt tcgggaacgt attccgagag atgcggatcg
     2581 acgatgcggc cgatccaggc gtcaagcccc cggtccttca tggtctgtcg aaatttgccg
     2641 agccgttcgg cgctcgattg gttcatgacg atgcctcaga tgaagaaagt cccgaagagc
     2701 ctgcgcaggg cggggcgcat cagaagagct tgcggacctg aacggtgacg gggaagccca
     2761 cgtgggagtc gctttcaagg tcctcgccga tgtcgagcct cagaatgcgc catccggcgt
     2821 cgcgcagctc gtcgagcgtg gcggagacgc cgaggtcggg gcaggcgaag ggcacttcgc
     2881 ccacgcgctg gagcaccgta acgggtgctt cggacgtgca cgtcatcagg gagcggttcg
     2941 gagcgtcgag gagcgggtcg tgcaggttgc cgtcggcgtc gcgcttcatg cgctgggcgg
     3001 ggccgctgat cgtgtcgccc gtgtagtagg cggtggagca ggcggaaagc aggagcatgg
     3061 cggcggtggc ggcgagaaga ctgcggacgg gaagcgaagc ggacattttc tggctgccgg
     3121 ccctcgtgtg aggaggccgg acgaactcga tcgggtaggt aaaacaaatc tattttagag
     3181 aaaaagcgcc ggatgtccgt aaaaacatcc ggcgcttctg taaacacttg tcggcgtgcg
     3241 gggcgctcgt ggatcagccc tcgcgcttct tgcggtagtt cctgcggctt cggttgaagc
     3301 cgcccttcac gttgccgttg acgtagccgc cgcggcggtt ttcgtcgggc atgatctgag
     3361 gcaggctgag ggcctgggag gacggatggt agggatccgt cggctcccag tggatctgct
     3421 ggtcgctggg cagcccgcgg agattctggc gcttgggctg gtagtggatc gaattgccga
     3481 agttgtcgtc gtcccagtcc tgctggcggg gctttgcctc ggcgtcctgc cttgcgggct
     3541 tcttcctgtc gcgcttcttc tcgaaaggct tcttctcgta gggcttcttt tcaaaggcct
     3601 tcttttcgaa ggtcttcttt tcctgaggct tcctgtcgcc tgcggccgcc tgttcggagt
     3661 ctgcgttctg aacgggcttt tcctgcgttc gggaggcttc gccctcaccg ttctggaact
     3721 tgcgcgactt gcggcggaag ttcttgaaat ggtccttgcg ggacttgtcc tgggctccgg
     3781 cttcggcggc ggggacggcc tgcgcctcga cgggtgcgga ggaagtttcg gcggcgtctt
     3841 cctgacgctg cttgaaggga cggttcttgc cgaagcggcg ctccgtgaag gacttgcgcg
     3901 gcttgcgggt ctgagcctgc tcggaggcgg cgccctcggc gggagcggcc tgaggctgca
     3961 taacggcatc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcggccttg
     4081 cgaggcttct tgccgaaggt cttgccctgc tcatcctttc tgaagtcctt gcggtccttt
     4141 cggaaaccat ccttcctgtc cttgcgctgg ccgttcttcg acttgcggcg ctcccagcgg
     4201 ctgcggcctt cgccgcggtc gtcctgctcg cccctgtcgc cctcggtgga ctcggccgtg
     4261 cggccgtatt tcgtgcggat cgtgagcttt tcgcggcgca gaagctggcc gataacggtg
     4321 cggggctcgg gaaggttgta cggattttca agcgtcaggc gcttttcggc ggcgacgagg
     4381 agcttttcaa agagcggaag cgtttcgggc gtgacgatcg tcacgagctc gcccgtgccg
     4441 tcggccgtca gggccaaatg ctcgagatag ggttcgggac cacctgagag gtcggtgagg
     4501 atcacgcggg aggcgcgatt gtgctcgagt tcgccgatga ccgagtgcgg catgacgagg
     4561 tgctcgacgc ggcctgcgat gaacttgagg acgagcgtct ctcgggccga ggcggactgg
     4621 gttgcggtgg cgcgcttggc ggcgatgccc gcctctttga gaaggcccgt gacgcgggtg
     4681 ctttcggtag gggtcgagac caggaagagt acgggcgtct tttcagcctc gcgggcgagt
     4741 gtctcgctca tcttctcttc gggtacgagt accacgcgtt ccttgggggc ggtttcacgc
     4801 gccgcctcgt cgggcgccct gcagtaggcg ggggcgtgca gcatggcgtt gtaggggtcc
     4861 tcggttgtct caacgacgac ggggcccgtc acgatcagct ggagggcggc gggaaagcgc
     4921 tcgacgagcg aagcgatgag gtccgtgtcg cccatgacgt cgtgccattc ggcgccgtcg
     4981 acgaggatcg tgcggaaggc ttcgggggct acgaaaccct tgtcgaagac ctttttcagc
     5041 accttgggat tcgtgacgac gaggtcgggt gcggtggcaa agatggcggc gaggcctgcg
     5101 ccgtcacgca cgcccgtcgt ggcggcgatc ctgaggccgg tgccctcgga catgcgggag
     5161 gccagcgtga aggcggtctc ctcggcttcg cgcgaggcgg cgagaatcag ggcggcgctc
     5221 ttgtcgcggc ggtcctgaag cttctgcagc agggccgtta ccgcggtctt gaggcgggac
     5281 gtgcagggcg tgccggagag gcgcgtgtcg cgttcgttca ggacttcgtt gagggtgctg
     5341 gagagaaacg gattggaggt cgcccagctc ttgaaggctt cgggcagcca ggacagcacg
     5401 cgctcgaaat tcgagatttt ttcgtctttt gtcatcgttc ttgagcaatg cacgccgaca
     5461 cggaatgcgc ggaaaagaag agggcgcccc ttcgcgtcgg ttgggtgtat gcggaatcga
     5521 aatcggatgt gcccgccaaa acgctgtggc gtgcgggagc agcctgtcag gaatagtgtc
     5581 tgcgggcgcg caggtgtgcg ccgcggtccg ttccgcgtgc cctctgatgc gaagggaggc
     5641 ggatctgcgg gatggcaggc tgaaggtcat cgcgtccgac ggtgaaggct tgtcggacgg
     5701 cacattcatt gtacacggcg gaaacgtcga atgcatccct gttgccagag caacggcttg
     5761 acgctgtaag attgtgtcat cagcaaccta cacacgaggg ctacacccat gcaggaagaa
     5821 ttgaaagatc tcaagatcga ggaactcgcc gaaggcaccg gccgtcaggc gatgaagggc
     5881 gatacgatcg ccgcacacta cacgggctgg ctcgaggacg gcacgaagtt cgactccagt
     5941 cttgatcgcg gcgagccgct cgagttcgtc tgcggcgtcg gcatggtcat caagggctgg
     6001 gacatgggcg tcgtcggcat gcgcgaaggc cagaagcgcc gccttacgat ccccgctcat
     6061 ctcggctacg gcgcctacgg cgtcccgggc tgcattccgc ccaacgctac gctcatcttc
     6121 gaagtcgagc tcgtgaaggt ctactaagac cggattgagg ccgcttcggc agtcatgaaa
     6181 aaagcccgcg tcagcgggct tttttcatga ctgtttgcaa caggtcgact tattgagcat
     6241 tgaacacggg aaggcccgca ttggcgagga gctcggccgt ctcgtaaacg gggagtccca
     6301 tgatgccggt gtagctgccc gaaatgttcg agatgaagat gctcgcaagg ccctggatgc
     6361 cgtagccgcc cgccttgtcg tagggctcgc cgctgttggc gtaggcctcg atttcgtcgt
     6421 gcgtgagctc gcggaagctc accgtggagt gcgaaatgcg ccagtcgcag gccgtttcgg
     6481 agaggccgac gtagacggcg gtgcgcacgt cgtgggtgcg gccggagagc ttttcgagaa
     6541 agaggcaggc ctcgtcgaag tcgcggggtt tgccgaggat ctcgttgccg aggctcacga
     6601 cggtgtcggc ggcgacgatg ggcaggcgca cgcccgcgaa ctcgcccgcg ttcttgacgc
     6661 cgaggccctc aaggaacttg ttgcgcgcgg tgcgcagcac gtaggccttg ggcgcctcgt
     6721 cgggcaggac gacttcgtcg ccctcgaagg ccatgagcgt atgggcgttg ctcgcgatga
     6781 cgcgaacgtc gtagccgagg cgtgtgagca ggtcgcggcg gcgcgggctc ttgctcgcaa
     6841 ggaaaatcgt tttgctggac atcggtgtca ggctcgatgg tagggatggc ctgcgaggat
     6901 cgaccatgcg cggtagatct gctcggagag catgacgcgc gcaaaggcgt gcggcagggt
     6961 catcgacgaa agccggatcg tcaggttggc cctggccttc acctcggggg cgaggccgtc
     7021 ggggccgccg atcacgaaga cgggcgtttc gccgtcggcg cgccagtgcg agaagtgtct
     7081 ggcgaggtcc atcgtcgtga gatccttgcc gtgctcgtca agcacgacga cgaagtcgtc
     7141 gggttcgatg gcggcgagga tgcgctcggc ttcagccgcc atgaggcgcg cgggcgtctg
     7201 gcctgcgcgc ggctcggtgc ggatttccct gagctcgacc ctgaagtcgc gcggaaagcg
     7261 cgacagatag tcggagacgg ccgactgcgc ccactcggtg atgcgctggc cgacggccac
     7321 gatgcggatc tgcatgactt attcgaagtc ttcgggcatg tggcccggac gatgcggcat
     7381 caggcgggag gcgcactctt cgttggcctt caggaagact tccttgccgc cccagatctc
     7441 ttcgaggttg tagtagtcgc ggacgttggg ctgcaggcag tggcagacga cggggccgag
     7501 gtcgacgagg acccattcgc ccgtttcctg gccttccatg ccgagaacgt cgccgccggc
     7561 ctgcttgact tcgtgcgaga cggagtaggc gagggcgcgg gtctggcggt tggaggtgcc
     7621 cgaagcgatg acgacgcgtt cgaactggtc ggtgaggcct tcggtgttga agaccttgat
     7681 gtccttggcc ttgacgtctt caagggcgtt gacgacgagc tgggtgagtt cttcgatgtt
     7741 catgtttcac ttgaggttaa tggcgcgcgg gctgatagag tccgtgcgtc cggatgtagg
     7801 aggcaacgcc ggggtcgagc cagccgtcga gcgacgacat ggcgcgggca aagggttctc
     7861 tggcaagcgt gcggcggatg gcggtggcgc tagcgcggtg aggcgccatt tcgaagcggg
     7921 cgatcctgcc cgaaggcgag aagacgagct ttcgcgcctc ggtcatgcgg ggcgcggccc
     7981 agagggcctg gctttcggga atttcggatt cgcgcacgcc gtccctggcg cagtacgcga
     8041 tgtgcacgaa gtcggtgagc gactgccact ccctccaggt ggagaggttg aaccactggt
     8101 cgctgccgag gatgaggacc agcggcatgt cgggcccgac ctcctcgcga agccggctga
     8161 ccgtgtcgat ggtgtaggtt gcaccgcttc ggcagagttc cgacgtgttg atgcggcatt
     8221 gaggctcgta ttctactgca atgcgaagca tgttcaagcg gtgaatgccg ggcgagattc
     8281 ggcttttctg ccaaggctcg cctgcgggca tgagctcgac gcgcacgagc ggaacggcgt
     8341 cgagcgccgc tctggccaga cagaggtgcc ccgcgtgcac gggatcgaac gtgccgccga
     8401 gaatgcccag gccgcggcgc gcggaatata gcgcaagact gtcggtcata gggtggctca
     8461 gaggttggtc acctgggagg agaacgtctt gtcgacgagg ccgtcgtcgt catagtgaac
     8521 ggccagccag ttgagctcga gctcctgcag tcctcgcttg ttgcgaagga agcgctggct
     8581 gaaccagtag tgccagcccg cttcgtcctt gtaggaggcg cggccgagaa gctcgttaac
     8641 gagtgcggac tccatgccaa gaccaatgcg gccgatctgg acgggaaggt gctcggcgtc
     8701 gagcctgcgg cagatttcgg ggaccgtgcg ctcacccgcc cactcgagct gcgcttcggt
     8761 cacgaagtcg ctgtcggtgg cgatgaacca gacgatcatg ggcttgccgt gggtggtgcc
     8821 cgtgaggcag agcgtgtcgc ggcggaagac gccgtcgccc tccgaaaggc gggttgcgcc
     8881 cgtcatggcg gcaagatcct cgagcctggt gctttcgagc acgatgcggg gcttgccgat
     8941 gcgggcgtcg cggccgagcg gcgtcgtcag agtggccggc gcgtcaagca ggcccgtcgg
     9001 gtaggcgaac gggccggtga ttttctcggc atgcgcgccg cccgcgagag cgagcggaag
     9061 tagggcgcct gccaggaggg ccgggagctt tgaaagaaag gcaggatgca tctgtgtggc
     9121 tgggtcgaac ggtgttggtc ggatgcgggg ctttgccctg aaggcacccc cgccgtggtt
     9181 tcagtatagc ccgcctgtcc cgttcggaca tcccctgcgg gagagcgaat caagaaagct
     9241 gcatgtcgcg ggcatgaaaa aggccgccac cctgcgcaga gggaggcggc cctgtgggac
     9301 cgttcggatc cgggctatca gcggttcagg gcgcggtagc cgatgtcgga acgcatctgc
     9361 atgccgtcga acttcacctg acgcgcggcg gcgtaggcgg acttctgggc ggcggcgatg
     9421 gattcgccga ggccgacgac gcagaggacg cggccgccgg agacgaccgt caggccttct
     9481 tcgttgaggg ccgtgccggc gtggaagacc atctgttcgg cggtgttttc aggcagctct
     9541 tcgatgacgg cgcccttttc ggggtgagcc gggtagccgc gggcggcgca gacgacgccg
     9601 agtgcggtgc ggcgatccca ttcgacttca acgctgtcga gcttgccgtc gatgccggct
     9661 tcgatgattt cggagaagtc gctcttgacg cgcatcatga tcggctgcgt ttccgggtcg
     9721 ccgaagcggc agttgaactc gagcgtgcgg acgatgggct tgccgtcggc gccgtgcgag
     9781 atcatgaggc cggcgtagag gaagccggtg tagcggatgc cgtcggcggc catgccctga
     9841 acggtcgggc ggatgatttc gcgcatgacc tgggcgtgaa tgtccggcgt gacgacggga
     9901 gccggggagt aggcgcccat gccgcccgtg ttgggaccct ggtcggcgtc aagaagacgc
     9961 ttgtggtcct gggaggtggc cataggaaga atgttttcgc cgtcgaccat cacgatgaag
    10021 gaggcttcct cgccgtcgag gaagtcctcg atcacgacgc gggcgccggc gttgccgaag
    10081 gtgtggcctt cgagcatcat gtcgatcgcg tcatgggctt cctgttcgga catggcgacg
    10141 acgacgccct tgccggcggc aaggccgtcg gccttgacga cgatcggagc gcccttgcgg
    10201 cggacgtagg cgtgggcggc ttcgggatcc gtgaaggatt cgtagtcggc cgtcggaata
    10261 ttgtgacgct tcatgaaggc cttggcgaag tccttggagc tttcgagctg cgcggcggcc
    10321 ttcgtcgggc cgaagatgcg caggccggcg gcgcggaact tgtcgacgat gccggcggcg
    10381 agcggggctt cgggacccac gaccgtgaag gcgatgttgt tgctcttgac gaattcgatc
    10441 agctggtcga tgtcggtgat cggaaggttt tcaagacggg gcgtcagggc cgtgccggca
    10501 ttgccgggag ccacgaaaac tttggtcacg cgatcgcttt gagcgaggcg ccaggcgaga
    10561 gcgtgttcac gaccgccgtt gccaacaacg agaaggttca ttttgatggt ctcttagaaa
    10621 cggaccggcg cggggcgccg gagttgtggg agagagctcc caggaaggtg aaattctgtc
    10681 gtgctccgtc gatgccggag ctcccggtgg gaacgcccta ttgtaccgcc cgaggcgtcc
    10741 ggctgtcccc ttgacgcgcc aggtaaaagc aaagcccgct caagtgactg aacgggcttc
    10801 ggcatcggac gcagtccgat tattcttcgt cgtcgaagtc ggcggacgtg tagacctgct
    10861 ggacgtcgtc gagatcctca agggcgtcga tcagcttctg catcttttcg gcatcttcgc
    10921 cggtgagctt gacttcgttg agggccttgt acgtgatttc ggagacgacg ggctcgatgc
    10981 cggcggcttc gaaggccttg gaaacggcgt cgaaggcggc cggttcgcac gtgacttcga
    11041 tggagccgtc ttcgtcggag acgacgtcgt cggcgccggc ttcaagggcg atctccatca
    11101 cggcgtcttc ggtcgtgacg ccgggggcga agacgaactc gccgatgtgc ttgaacatga
    11161 aggagacgga gccttccgtg ccgaggttgc cgccgtgctt cgtgagcgcg tggcggacgt
    11221 cggcgaccgt gcggacgcgg ttgtcggtcg tgcagtcgat gatgagcgca gcgccgccga
    11281 tgccgtagcc ttcgtagcgg atttcttcgt aggagacgcc ttcgagctgg ccggtgccct
    11341 tgagaatggc gttggagatg gtgtccttgg gcacgttgcc gccgcgggcc ttgatcaggg
    11401 cgaggcggag acgggagttg gactccggat cgccgccgcc catgcgggcg gccacgatga
    11461 tttcacgaac cagcttcgtc cagaggttgg agcgcttggc gtcctgacgg cccttgcgat
    11521 gctggatgtt ggcccacttg gaatgacctg ccatgatgat cctttcgata tgcagtaaac
    11581 ggccgggcaa cccgtgcctc tcgggcattc atggtgcgtt gccgcgcgcc tgtgttcttt
    11641 gggaatgagg gtcctgctgc cggcgaactc tgtgctgtgc gcatgggccg gagcggataa
    11701 ggtcgagtat tttaaaacaa aaaagaccgg cccgcgacag gcggaccggc ctttcggtca
    11761 gatgacctgc tgatgaatta cttcaggatc agcttgatgg cgttcgccca gacggcgaga
    11821 cccttgtcga gcgccttttc gtcgatgtcg aacttggccg tgtggtggcc ggacggacga
    11881 tcggcgccga gcacgaagaa cgcagccttg ccgccgtgcg cctgaacgcg acgggcgagg
    11941 atggtggcgt cttcagagcc gccgaagttc atcggatcct gacgggcggt catgccttcg
    12001 acgttgccga cggcttccgt gagaaccttg acgagttcgg ggtcgttggt gaggtcgacg
    12061 gcctcgccca tcttctcgat ggtgtactcg cagttctggc tcagagcgca acccttgatg
    12121 atgttgacgg cggaatcgta catgtactgg ttgatttcgc cggtttcacc gcggacttcc
    12181 atcttcatga cggcgtgcga cgggatcacg ttgcggcctt cgccggcaac gagctggccc
    12241 acgttgatgc gggtcatgcc gctgccgtgg cgggcgatgc cgagcaactg cacgaaggcg
    12301 ttgcaggcgg cggccatggc gttgtggccg gcgttgggtt cgacgccggc gtgagccgga
    12361 cggcccgtgt aggtgacgtc gagcttcgtc gtgcagagga agccgtaggg gttgacggag
    12421 acttcgccgg acttgcacat catggcgatg tgggcgccga ggaagtagtc ggcgtcgtca
    12481 aggatgccgg aggcggccat gccggcggcg ccgcggacgc cttcctcagc cggctggaag
    12541 aggatcttga tcttgccgtt catctggtcg acgttgtcgg cgaaccagtg ggcgacggcc
    12601 aggccggtgg aggtgtgggc gtcgtggccg caggcgtgca tcaggcccgg acgcttggag
    12661 atgaagcctt cgcgagcggg gatgtggcag tcttcggtgg attccgtgac ggggacgcag
    12721 tcgatgtcga agcggatggc gagcgtgggg ccgggacgcc ccgtgtccat cacgccgacg
    12781 cagcccgtga ggccgtccat ttcggcgagg agctcttcgc tcacgccgtt ctcgcgggcg
    12841 agcttgaggc cggcttcaac aaccttgagg ttgcggccga ggcagttgtc gggattgatg
    12901 actttcttgc cgagcagcac ttcgtagccg tattcacgca gcttgccgac gatgaaggcg
    12961 gtcgtggtga attcgctcca gccttcctct ggcatcgtgt gaagatggcg gcggatttcg
    13021 atcagctggt catggtaggc gcaggtgcag gtcattggac ttctcctcaa tttccataaa
    13081 gtgcgtcctc tatcactttc ggggtattga aggcgaaagc gagtcgaggg caaaggtcga
    13141 taagagaatt atgtgcctct tggtggaaat ccgcatcagg ataaaccgta aggcggactg
    13201 cctagggatc gttcgttttt ttgcctactc gcgcacgcct caagcctaaa agcaacatga
    13261 gaaacgtgaa gcctccccgc tcctttatct cgtgtcaaag aaatggtgct ttccttctct
    13321 ctcttagggg gtaggctgta atcccttggt agtatttcca acatgggccg actgcatgcg
    13381 ccggaaagag tgcacgtaaa agacaacctg aagccctgaa aacgacgatc cgacaatgaa
    13441 ggagacagca aatgtcagtt actggactgc agaccgaagg tctgaagtat gcaaaggcga
    13501 ttttcgacac cgtccgccgc atgtcggcgg acacgcttgg cgtttcccgt cagggctaca
    13561 gcgagcttga gacggacgtc ctgcactacc tgcagggcct tggccgcgac atcggccttg
    13621 aagtccgcgc cgacgacgca ggcaacgtct ggatgacgat gcccggccgc gaccgcacgc
    13681 ttcccgcctt cgttgccggc agccatgttg acagcgttcc gcagggcggc aactacgacg
    13741 gcctggccgg cgtgaccgcc gcgctcacgg tcgcttggtg gatgcaccgc catcaggtcc
    13801 agctcgagcg cgactacacg gtcctcatga tgcgctgcga ggaaagctcc tacttcggca
    13861 aggcctacgt gggctcgctc ggcatgatgg gccgcctgac gcagtccgac ctgatgctgc
    13921 gccaccgcac ggaggacatc acgctcggcc agtgcatctc ggcctgcggc cttgatccca
    13981 gcaagctcac cgccggcacg ccgatcgtcg atctgaagaa gatcgcctgc ttcgtcgaac
    14041 tgcatatcga gcagggtccg acgctcacga gcaacgacaa ggtccgcgtc ggcgtcgtca
    14101 cgggcatccg cggcaacgtc cgccacaaga acgtggtgtg ccacggcgaa acggcccact
    14161 cgggcgccgt gaacaaggag ttccgtcatg acgccgtgat ggccacggcg gcgcttctga
    14221 gccgcatgga taatcactgg caggaatggc tcgaccgcgg cgaagacctc gtcttcacgg
    14281 tcggcgtcat ccgcacgggt gcgaccgccg ccatctccgt cattcccgga gaagtgagct
    14341 tcacgcttga catgcgcagc ctgagcgccg acacgtgcga gcgcttccac cagctgctgc
    14401 tcgaagaggc tgccgccctt gaaaaggaac gcggcgtgcg cttcgacttc gacaagccgc
    14461 tctacacggc ccccggtctc gtcgatcagg ccctctccga ccgcatctac aaggccgccg
    14521 aagacaacgg cattccgtgc atgcgcctgc cctcgggcgc gggccatgac gccgccgtga
    14581 tcggcgccgc cggcattccc gtcggcatga tcttcatcgc caaccagaac ggctcgcaca
    14641 atcctcatga ggaaatgatc atgaaggatt tcatgacagg taccgacctc ctttggcaga
    14701 cggttcgtca gttcgactaa aggtggttgc tatggacagg agcaagctga tctcggtcgt
    14761 gctcgtggac gagtatccgc tctttcgcaa ggcgatggcg gatctcctga acgcaaccgg
    14821 ggagtttcag gtgctcggcc agacgggcga gggcgacacg gccctcggcc tttcctcgct
    14881 cggccccgac atcgtcgtga tgagcctcga ggccgaggag tactctgcgc ttgatcttct
    14941 caaggagatc aagtaccgcc agcccgccac gcgcgtcgtg atggtgatga gctcggctga
    15001 cagttccggc cagctcatgc gggccatccg ccttgaggcg aacggctacc tgcttcgcac
    15061 gattacgccg agcgacttcg tcgaacagct caggaagacc gcgcagggcg gcatggccgt
    15121 gagcgagaag gtcacttcgg cgcttgccga aaagcttcgc ggcgactcct acgccgtcga
    15181 ggaggtccgc cgcgtgagcg agctgaccag ccgcgagcag gacgtgctcg gctacgtggc
    15241 cgcggggatc tcgaacctcg agatctccaa gtgcctgggc atttccgagg gaacggtgaa
    15301 ggttcacgtc aagcacacgc tcaagaagct caagttccgc tcccgcgtgg agctcgccgt
    15361 ctgggcgtcg gaacacggct tcaaggtgaa gaccgcaggc aggttctgag cgaatctgca
    15421 gtaatcccaa aactgaactg aagaaaaggt gcgctttccc gctgggaggc gtgccttttt
    15481 gtcgtccatg cgcagggatg gatgtagggg cctgaagaag cggcgcgagt ttgcgtgcgt
    15541 gcgtttggcg ggcggggcct ggggcgtgac ggtcgtctga tgatagggcg gaaatgaaag
    15601 agggaaaaga aggaaagggc ggtcctgcgg ggcgaggagg cggcggggaa gacctgaaaa
    15661 tgcaaaggga caatgctttc gcattgtccc ttcactgtgc ttgttggccg gagccggtgg
    15721 tctgccggct caggttgcat ctgttgtcct cagtgtagtt cttagattag atgaaaccgt
    15781 agatgaggcc aaggacatag cccgttgccg tggcggtcaa cacgccgatc agacccggaa
    15841 gaatgaagga gtggttgatc acgaagcggc cgatgtgcgt cgtgccggag cggtcgaact
    15901 ggagagcggc caggtcggac gggtaggtcg gcaggatgta gtagccgtag caggcggcgg
    15961 cgcaggaaac cacgaggccc gggggaacgc cgatggagag ggcgaccgga acgatggttg
    16021 cgacggcggc agcctgagag ttcacgagct tggacgtaag gatcaggacg agagcgtagg
    16081 cccagggata ctggcgaacg atttcggtca tgacggcctt gaggtcggca aggtgagcgg
    16141 caaacatcgt gtcagccatc caggccacgc cgaacacggc ggtgatggcg acgcagccgg
    16201 cggagaagac cggggtcttg ccgatgctgg aagcgcgggt cttgcagaag atcacgatga
    16261 aggcaccgca ggcaagcatg aacatctgga tgatcagcgt catggagagg gccttcttgc
    16321 cgatgatcgg gcgaagttca gggaacatgc cgaagacggc gacggcagca accgtggcga
    16381 ggaagatcca gagggaggcc cactgttccg gacggaaggt cttgccgatg agggtcgtcg
    16441 tttcgccgta gacgtacttg cgctgttcgg gatcggcgat gagcttctgg aattcttcgt
    16501 ccttgtcgag gtccttgccg cggaacatgg agaagacgga gatcgcgaag aggccgacga
    16561 gggcgcccgg gatcgtgatg ctgaagagct ggatgaagcc gaaggccttg ccaccaacct
    16621 ggtagccgtc acagagggcg atcatcgaca cggcggcaac agcggccggc gagcagatga
    16681 tggacatctg ggaggcgatg gtggaagcgg ccatcgggcg ttccggacgg atgccgttct
    16741 tgatggcgac gtcgtagata atcgggagca tcgtgtacac aacgtggccc gtgccgcaga
    16801 gaagggcgag gaaccagcag aggtacggag cgaggaagca gaccgcgcgc gggtgcttgc
    16861 ggagcatctt ttctgcgatc tgaaggaggc agtcgaggcc gccggcagcc tggagggcgg
    16921 cggaggcgca gatcacggac agaatcgtca ggatgacgtc gaccgggggt ttgcccggct
    16981 tcagatggaa gccgaagacg aggatgatga ggccgatgcc gccaatgagg ccgagggcca
    17041 tgccgccctt gcgggcgccg tagaagaggg caaccagaac gatgaccagt tgcgcccaga
    17101 agtcaaaacc gagcatgtta attctcgcag tcaagttgtg aggacattgc taattgtgtc
    17161 gattcttcta cctctggggg gataattctg atcttctttt gacctatgga cataaatcgg
    17221 ggggatctcc ctttttcttt acagaaaaat cttgttactg atggtgtaaa ccgcctaggg
    17281 cggttccctt atcctgtcgc acaagggtga ttttctcatg ggtttcccta gcgggagaag
    17341 gaatgtggaa aacgggggag caattgtaag aaggggtgtc gaacgtcgag gcagggttgc
    17401 ggcgcgtgac ttctccgccc gtttttgcct tcagatgatt tggatttggg ttaaccctaa
    17461 cctctaaaag ggtattccca aagtgaaagg gcgtcctttc gtatgaaagg acgcccttgt
    17521 gacttcagtt agtcagtgag ccggagatca ggccttcttg caggcgatcg tgacaggctt
    17581 gagctgctgg aagaagtcgt tgcccttgtc gtcgaccagg atgaaggccg ggaagttttc
    17641 gacgcggatc tggtagacgg cttccatgcc gagttccgga tactcgatgc attcgatgct
    17701 cttgatgcag tcgctcgaga gcacggcagc gacgccgccg atggtgccga ggtagaagcc
    17761 gccgtgcttc ttgcaggcgt cggtgacgac ctgggtgcgg ttgcccttgg cgatcatgac
    17821 catggagccc ccggcagcct ggaacggatc aacgtacggg tccatgcggt tggcggttgt
    17881 cgggcccatg gagccgcagg cgtagccttc cggcgtcttg gcgggaccgg cgtagaggac
    17941 tggatggttc ttgatgtagt cgggcaggcc ttcgccgcgg tcaaggcgtt ccttgatgcg
    18001 ggcgtgggcg atgtcgcggg ccacgatgat cgtgccgttg agcagcaggc gcgtggagac
    18061 ggggtacttc gtcaggtcgg cgagaacttc cttcatcggg cggttcaggt cgatggtgac
    18121 gccgccggct tcacccggct tgcggagttc ttcggggatc aggcgggcgg gattgtcgtc
    18181 gagcttttca acccagatgc cttcttcgtt gatcttgcac ttgatgttgc ggtcggcgga
    18241 gcaggagacg cccatgccga tcgggcagga ggcgccgtgg cgcggcaggc ggatgacgcg
    18301 gatgtcgtgg gccatgtact tgccgccgaa ctgggcgccg aggccgatct tccaggcttc
    18361 ttcgaggagc tgctgttcga gctcgacgtc gcggaaggcg cggccggttt cgtcgccggt
    18421 ggtgggcaga ccgtcatagt agtggcagga ggcgagcttc accgtgagca tcgtgcgttc
    18481 ggcggaggtg ccgccgatca cgaaggcgat gtggtacggc gggcaggcgg ccgtgccgag
    18541 gctcttcatc ttttcgatga ggtagggaac gagcttggcc gggttgagga cggccttcgt
    18601 ttcctgatag aggtaggact tgttggcgga accgccgccc ttgaccacgc agaggaactt
    18661 gtactcggcg ccttcggtgg cttcgatgtc gatctgggcg ggaaggttgc acttcgtgtt
    18721 cacttcctcg tacatcgtga gcggagcgtt ctgggactgg cggagggctt cctcggtgta
    18781 ggtcttgtag acgcccttgg aaatggcttc ttcatcgcag tagccggtcc agacctgctg
    18841 gcccttttcg ccgtgaacga tggccgtgcc cgtgtcctgg cagaacggga ggatgccctt
    18901 ggcggcggtt tcggcgttgc ggaggaacat cagggcgacg tacttgtcgt tggccgtggc
    18961 ttcagggtcg gagaggatct tggcaacctg ttcgttgtgt tcgcgacgga gcatgaagtt
    19021 cacgtcgtgg aaggcgcggt tgatcagctt ggtgaggcct tcggcttcga ccttgaggat
    19081 cttcttgcct tcgaattcac cgacgctgac gtggtccttc gtcagaagat agtattcggt
    19141 cttgtcttcg ccggtctgga acatgggcgc atatttaaat gcgggagtct gagccatgat
    19201 gtctttgcct tattcagtaa gtgacggccg caggggtttg gcggccatga tgagcgtccg
    19261 ccttgtgaca aagttttgcc actggtccgg gcggacgatt tctatcacca attatctacc
    19321 tccttctaag gagactgaat aaggaggatg ccttagttcg ggcaagaatc aaggatcctt
    19381 tgataaggag gtaggttcgg gggcgttctt aacgtttctc catgcgcccc taggaaaatc
    19441 aggcgttcat gagcttgccg cgctcaagcg tgaggatgcg gtcgacgttt gcggcgggga
    19501 tgacgccctc ggcggccacc acggcgatgt tgcgggtgtc tcgtacccac ttggaaatac
    19561 aggttaatac tgatgtcgcc gattcggctg aaagacagcg ggaaggctcg tcaagaagga
    19621 tgacgcgcgg ctgctgtacg aagagctgcg cgacctcggc gaggcggtac tgttcgggcg
    19681 tgaggacgga gatcggggag tgtgcgaggg gctcgagcct gcagcgggcc agtgcgtcgg
    19741 tgacgcgttc gcctgcatcg ggcggaagct gccaggtgtt ctgcatgagg cctatctgca
    19801 ggaactgcgt gaccgtcatg tagggggaga tgaggctgcg cgaaaggggc tggagcaggg
    19861 cgcccgcgcc atcggcaccg aagcgcctgg cgccctcggt gagctcgatg ctgcccgtgt
    19921 gcccgatcat gcccgagagg gccttgagaa gcgtggtctt tcctgcgccg atcacgcccg
    19981 ttacaagatt gaccgtcgcc gcggtgagcg tgagggagag gttgacgacg gcgggaatgc
    20041 cctgataggc gacactgaag ttggaaagac gaagctgaag catggcttga gggtgtttga
    20101 tcacggtgtc agccatgata ggcacttcgc cctacgaaga aaatcggtcg aaagtcggtt
    20161 tcgattagtc cgttggtata ggttccgatg cgggtgagcc ctcatgttcg gccgtccgtg
    20221 cggctgtcga tcgaaggaag accgtctcgc aggccctgac gccggcgggg cgcgtttcga
    20281 cggcgtgaat gagcggcacc ggacggcggt cgagataggt gcgggtcacg acgagtgcgg
    20341 gagagccctc gggcacgccg aagagcttcg agcacgcctc gtcgagtgcg gcggcttcgg
    20401 cgctctggtg catggagcgg cagcgcacgc ccgcatgctc ctcgagaagc gcaaggacgg
    20461 agcgcgggct ctgcggcgtg aggtatggga tgaggtccct tgcggcgtcg cgcacccaga
    20521 gctcggtgcg ggcgacggca cgaccgtcgg gtcccttcgt gagcatggtg agcgtgatga
    20581 gcggggtgag aagcgcaaag cccgtgcggg cggccgtgtc ctcgtcgcag atcatgcggc
    20641 ggatgctcag aagcgtgcgc ccgcctgcgt cggcagggag cgacgggacg aggctggccg
    20701 tgaattcggc tccgcgtccg tcacccgtca cgatcgtgcc gcggcgcgga gagcgcctga
    20761 tgagccccag atggacgagc tccgcgagtg cggagcgcac ggtgtgcctg ctcatgccgt
    20821 agtcgttcat gagctgggcc tccgtgggca ggagactgcc gacggggagg cgccccgaga
    20881 gtatgtcgcc tgcgagcgtc tgtgcgaggc ggctccagag aggcgtgcgg gtggatttga
    20941 tggtggtcat gccgagaggg tatgaaaaaa ctcccgccgg agcaagtccc gcgggagttc
    21001 ttttttggtc agtattcagt tggctgaagg agaacttcgc tgtcagcccg agtactgatt
    21061 acttccacca gttgttttcg tcagcgagga cgtaacgcgt ggcttcctgg ccgttctcga
    21121 tcagttcagc gccgatggag tagttcttcc aggcggtgag cgtgccgcag acgatcatga
    21181 gaagggcgat gaagaggaag gtcttacgaa gaccggcgcg gtcgccgttg cgctggtcga
    21241 tgatgtccgt ttccgggttc ttggcgaaga tgtcccactt gacggcgagg cggtacagac
    21301 cgatcatgcc gtggagttcc gtgatgacga ggaacacgaa cgtgtagatc atgcccatgt
    21361 gatgggtgtg gacgccgatc aggttcacgt caaagccatg cgggttgcag agcatggaga
    21421 tgaggtgcgg gaacacgaag atgaagagga ggaaggccgt gacgagctga accatccaga
    21481 gcgtcgtgtc ttcatgacgg agcatcttgt agtgagcctt gatgtcacgg agctgatggt
    21541 aggacgtcgg gaagcggcga agggcgcaga aggcgtggat cacgacgcag agcgtgatga
    21601 agccgacgaa gacgacgtgc agccaagcct gttcttcagc gaacatgaac atgccgccgg
    21661 aggtgttgat caggttggcg aagaggtcct tgctgatctg cacggaggac gtgaaggcca
    21721 tgtggcagaa caggaagatg ccgaggatga cgcccgtgac ggactgggcg acgtcggcga
    21781 gagccgtgga cggagactga cgcttggcgt ccttgaggcc ggcgccccag acgttttcag
    21841 tcgcggtcgg ggtgttggag tacttgacgg aatcaggggt agacatttgt ctttcctttg
    21901 tgagctttgt ttggttcatg gaatccgggc ggatgcttga cagggtccgc gccggttcta
    21961 tgggggtcta agcgccgacg aaagccggca gatcatgtca gaccgtatga tgcgccctta
    22021 aggattatgt aagtttgact tttatcaaat gaaatgagac taggcgagtg gcttcgagtc
    22081 cgatttttgg tagttttgcg tagttatacg gagttgaagc agtcaaaaat actcacaaat
    22141 agcaatactc aagggtaaac ccgtaaaaat tatggtcaaa aaaatcatga aactggcggg
    22201 caaaaaaaga cggccggatg tccagccgtc ctgaagtgtt gaggcgcctt gttagagggt
    22261 gccgaagatt cggtcgcctg cgtcgccgag gcccggcacg atgtaggcgc gatcgttgag
    22321 cttttcatca agggctgcaa cgaagatgtc gatgtcggga tgggcttcgc tcagaacctt
    22381 gatgccttcg ggggcggcga cgagcgccat gaagtggatc tgctcgcccc tgacgccgcg
    22441 tcgcttgagg atctcgatgg cgtaggcggc ggagtggccc gttgcgatca tcgggtcgac
    22501 gacgatgtag ttgctgttgg gagcgtcggg caggcgcacg aggtattcga cgggttcgtg
    22561 ctgggcgttg cggtagacgc cgatgtgacc gacgcgggcg gcgggaagca gggcgaggag
    22621 gccgtcggtc atgccgaggc cggcgcgcag gactggaacg atgacgggat ccttttcgac
    22681 gagcatgggc gacttggtcg cgcagatcgg ggtttcgatg tcctcgagct tcgtcgggaa
    22741 gtcgcgggtg acttcgtagc ccatcaggag cgcgatttca tggagcagcg tgcggaagtc
    22801 ctgcgtgccg gtgtttttgt cgcgcatcag ggagagcttg tgctcgatga ggggatggtc
    22861 gacgatgtga agacggggaa agcgggaatc ggtacgcatg aaatgttgcc tcagtcgtgt
    22921 tggccgccgt ccggtcggac ggggcgtggg cggagcgtgg gaagggcggg tgcgcttcgg
    22981 gaggcgctcc gtcagaatta gcctaaccta acactatagt attgggatcc cgatcagtca
    23041 aaggcgtttt gacggagttt cgggcaatta ccctgcctcg tcgcactgtg catttgctat
    23101 gatgaacccg aaatagcgcc gttccggcga cgccttgggg gcggagcgcg ggacagttgt
    23161 ccgtgaagcg gggccgtgca tgcagcgcgg cgttctcagt tcgtcttcgt cctgccgatc
    23221 acttcaggca ggacgcttcg gagcccttat gaagattcgt accatttttc tgctgttcgt
    23281 cctcattctg atggcggtct ttctggtcat caactggacg gcgctctcga ccgtcacggc
    23341 cgtcaacctg atctacaccg aaatccaggc tccgctcggc gttctcgtcg tgggcggttt
    23401 cgccgcggtg gtcttcgtgc tgctcgtcta catggtctgg cagcaggcca gcgtcacgct
    23461 tgagcttcgc gccgcctaca aggaggctcg caacgcccgc accgtggctg acgacgccga
    23521 aaagagccgc tttgccgaaa gcaaccgcat tctgctcgag cgcattgaaa agctggaggc
    23581 cctcatgacg gcccgctccg acgagacgct caccgccgtc cgtcaggagg ccgtccgtgc
    23641 tgaagagcgc tacaaggctc tcttcgacga ccagaagagg gctcagggcg agctgaccgc
    23701 ccaggtccag acgcagatcg gcgccatgga aaagcgcgtc gtcgagacgc tgccctccgc
    23761 cgtttccgcc gccgtgccga cgcaggctct ggctgaagaa gctgaaaaga aggcggacgc
    23821 cgaagaaagc tgcgcccgca agagcgtctt caaggacctc ttctaagtct cctgacgaca
    23881 ccgtcacaat gcaatcgggg cgcctccttt ctgggaggcg ccccgatttt tcggtgtgta
    23941 tcccgcctgg gcggaggctg ggattattcg gcgtggtagg acgtcacgcg ctcgacttca
    24001 tcggccgcac cgaggaagac ggccacgcgc tggtggatct tctcgggaac gatgtcgagg
    24061 atgcgctggt ggccgttcgt ggcggcggcg ccggcatttt ccatgagcat ggacatcggg
    24121 ttggcctcgt acatcaggcg aagcttgccc ggcttcgtga ggtcgcggtg gtcgcgcggg
    24181 tacatgaaga tgccgccgcg ctgcaggata cggtgcactt cggccaccat ggcggcgacc
    24241 cagcgcatgt tgaagtcctt gccgcgaacg ccggtcttgc cttcgaggag ctcggcgacg
    24301 tagcgcttga ccggatcctc ccagtggcgc atgttggagc agttgatggc gaattccttg
    24361 gcggagcggt cgacgcggac gtcttcggcc gtgagcatga aggtgccggt cgtcgggtca
    24421 agcgtgaaca tcacgacgcc gcggccgaac gtgaggacga gctgggtctg cggaccgtac
    24481 atgcagtagc ctgcggcgac ctgcttggtg ccgggcatca ggaagtcctc ggccacgggc
    24541 tcgcgcttcg ggcgaacggc gggaaggacc gagaagatcg agccgatgga gacgttgatg
    24601 tcgatgttgg aggaaccgtc gagcgggtcg aagcagatca ggtacgggcc gatcggcagg
    24661 tcttcgggaa cgggaatgac ctcggggatc tcttcgctca ccatgccgca gacggagccc
    24721 gaaagggcga cggcctcgac gaagatgtcg ttgctgatga tgtcgagctt cttctggtct
    24781 tcgccctgga cgttttcgga gccggccatg ccgagcacgc cggcgagggc gccgttgcgc
    24841 accttgaagc tgatcgtctt gcaggcttcg gagacgctga cgatcaggcg ggagagaacg
    24901 gggtcgaggt cgtgaagctg ctgttcacgg cagagatact gactgagaga aagggtggac
    24961 acgatgcaat ttcctcttat gcgggattgt tcaaagcttt ttcaacgatt tcgcgaacgc
    25021 tcacggagag cttctcctga cgttccagat gcttgagcgc ctggaacatg aggttcgaga
    25081 gctgcggcgt gtagcggcgc cagttttcga gactgcgggc gacgcgggag gcaacctgcg
    25141 ggttgagggc gtcgagcttg agcacgcact cgacccagaa cttgtagcct tcgccgtcca
    25201 gacgatggaa ttcggcgggg ttgttcatga agaaggccac gagcagcgcg tagacgttgt
    25261 tcgggttcgc catggagaag aaggggctgt tggcgagttc ggagacgcgg gtgacgacgg
    25321 gggcctcgcc cgcaaagcac ggggcggtcg cctgaatcgt cagccacttg ttgacgagaa
    25381 gcggctcgga gtaccactcc ttgagcgccg agacgatggt gtcgttcttg gtcggcgtgc
    25441 tcgagcctgc gatcatgttg agcgcggcga ggcggtccgt caggttgttg gccgtgttga
    25501 actggtcgcg aacgcgcagc accatcgtct tgttgcctgc ggcgacgccg tagtagtagc
    25561 aggtgttctt cagggcgcgg atgccggcgc tcgtcgagtc gggagagtat tcaccctcaa
    25621 gcctgcagcc gtcgatgacg ctcacgagct tgctgtgcca gcggatgccg agggcttcgc
    25681 ggacgatttc gcgcgcacgg tggatggcgg cgggatcgat gatcggcatg gcgtcggcga
    25741 cggcacgctc gctcgggagc tccatcgcga ccgcacggaa ggcgggcgaa aggttgacgt
    25801 cgtcgatgat ggcgccgaac gcctcgagaa gcgttgcatt gacgggagcc tggtcgttgg
    25861 cgagaacggc gcgcacctgc tcgaggatcg ccttcatcat caggcggttc atggcctccc
    25921 agcggttgaa gggatcgctg tcggcgcggg caagcagcgc aagcgcgtcg gcgtcgaggc
    25981 tgacgtcgag gatgaccggc gcggcaaagc cgcggttgag gctcggaacg ggtttttcgc
    26041 gaaggcccga gaagacccag gtctggcgtg cgtccttgag ctcgaacatg cgcgtgagct
    26101 gggcggagcc ttcggactcg cctttgagca gaacgggcat ctcgcggccc tcggcgtcga
    26161 gcaacgcgac ggggaagggc atgagcatcg gttccttggc gggctggccc ggcgtgggcg
    26221 gcgtggtctg ctcgaccgtc atgacgagct ggcccgcctc ggcgttccac tgcgtggaga
    26281 cggcgacgcg cggggtgcct gcctgagagt accagcggcg gaactgctgg aggtcgcgac
    26341 ccgaggcctc ctccatggct tcgaggaagg cctcgcacgt cacggcgtgg ccgtcgttgc
    26401 gggcgatgta gaggtcgaaa cccttgcgga aggtgtcctt gcccagaagc gtctggagca
    26461 tgcggatgac ttcggcgccc ttttcataca cggtgctcgt gtagaagttg tcgatctcct
    26521 gatagctgtc gggacggatc gggtgggcca tggggcctgc gtcctcggcg aactgggcca
    26581 gacgtagcgc cttgacgttg cggatgcgct cgaccgcgcg ggcggactcg tcgccgagca
    26641 tgtcggcgga gaactcctga tcgcggaaga ccgtcaggcc ctccttgagc gtgagttgga
    26701 accagtcgcg gagcgtgaca cggtcgcccg tccaattgtg gaagtactcg tggccgacga
    26761 cggactcgat gttggcgtag tcctggtcgg tggcgacctt cggattggcg agcgcgtagc
    26821 gggcgttgaa gatgttgagg cccttgttct ccatggcgcc gaagttgaag tcgttcgtcg
    26881 ccacgatgcg gaagccgtcg aggtcaagct cgagccccca gcgctcctcg tcccagcgga
    26941 tggacttctt gaggctgtcc atcgtgtgct gcgtcttgtc gatgtcctgc ggctcgaccc
    27001 agacttcaag gctggcggtg cggccgtcct tgaggacgat cgtgtcgcga agggcctcga
    27061 gcctgccggc gacgagcgcg aagagatagc agggcttcgg gaacggatcc tcccagcggg
    27121 cctcgtggcg gccgtcgtca aggtccttct cctcaatgcg gttgccgttg gagagcagca
    27181 cggggtactg ctccttcgac gcgcggatca cgacgctgta gcgggagagg acgtcgggcc
    27241 tgtcgaggaa gtaggtgatg cggcggaagc cctgggcctc gcactggctc atcaggttct
    27301 ggcccgaggt gtagatgccc gagagcgcct tgttggcgga cgggctgaag cggttgacga
    27361 cgatgacgtc gcactcgcgg tcgatgccgc ggatggtgag caggtcgtcg ccgagcgtgt
    27421 agcgggactc gtcgagcgga aggccgtcga cggcgacgct gacgagcgtc agggtttcgc
    27481 cgttgagaat gagatcggag gaatgctgtg catcgggatt cggacgcacg tgcatggtgt
    27541 tgatgacgct cgtgctgtcg gcgtcaaggt caaactccag atgcaccgtg tcgatcagat
    27601 gggacggcgc gcggtagtcg gcgcggaaga tggtttctgc catttgtgct cgcaatatgt
    27661 ggacgcggcg gagcacgtcc gccgctgaca aagcaatggg tttctagcga ttgtagacaa
    27721 atcggcgggg acctgccgcc cgcgtccgcc cgatgcggga aatgcaaaag accccggcgc
    27781 tctttcgggc gtcggggtcc agatgattcg cttttcagga aatcaggctt tcgaagggat
    27841 tacttcgcag cggtttcctg agccttggcc atttcggcgt cagcggcggc gtagtcgtga
    27901 ccggccttcg gatggcaggc caggcagtcg gcaggcttct tcggatcctt gtcgagattc
    27961 tggtgcatct tggcgatgaa cggcttcttg gcgttgtgca tgcgggacag gtcatggcag
    28021 ccgcggcagg cggagaagtt gcgttcgatc atctgcttgt gaacggcttc agccatttcc
    28081 ttgcgctttt cgagctgctt ttcatgcgtg ctaagagagc cctgaatctg gccgagaagc
    28141 gagatggagc cggcacgggc cttgtcgaag agaagagccg tgtagtcgag cgggccgacg
    28201 tgttcgttgg agtggttcag aaggtggcag tcggagcagc cggcggtggc gccggagctg
    28261 gtcatgtagt gctggccctt tttgtaggcg gcgtaggtga gatccatgga gtggcagaat
    28321 tcgccgcaga acttcgggct gcccgcccag tggacgacgc tcgtgaggat accgacgaag
    28381 acgatgccga tgacggcgcc gatcaggagg acgccggcga cataccattt gctgttgttg
    28441 gccattttgc tggaacctgt ttgtaagagc ggccgggggc ggtgtgcgta ccagggggag
    28501 ccatccggcg ctgtttaggg gtgaaaaagg gcagactatg cctagggaag cagagtctgc
    28561 acgtgtgtaa tcttattaag gtttatgtaa ggaagggtga ggtgagcttg attgccgtca
    28621 acaaaggcgc gctttcatgg ccttgagacg cgcttggccc tttccttcca tcagtttcag
    28681 gagttcagcg caatgaccac ggcttcgggc gcaacggtgg cgacggcgcg catgccgatg
    28741 tgaagcccgt gactgggtct ggcgaagccc acgatggtga ggcccgaggg cagctggatg
    28801 gtgcattcgc cgcccttgtc ttcgcgctcg ctgcgaagca cggcgcccac gatctggttg
    28861 tcgcggcttt cgacgagggc ggggttgtcg gcggagatgt ccactgcggt ggccttgcag
    28921 agggcgagga cgtgcttgcc ttcgcgaagg ccgaggagct gagcggactc cttcgtgcag
    28981 gaggcgcgga tgtagtgcgt atcgtcgatg gagaggatga ggcgaacctg cgccgagccg
    29041 atcttcagct tctcgatgac ggcggggaac tggttgcgca tcgaggtcct cagcgagctg
    29101 acgccgacgt tcttgagcgc gggagagtcc ttgagacgga agcgtacgag cacctcggcg
    29161 cgggcgcggg tcagctcgtc ggctagcttc agcacctctt cgcccgtggc cgtgagcctg
    29221 gtgccgccgc ccttgttgcc gcccacgacc ttctcgacca gaggggcgcc cgccagattg
    29281 ttcagcgtct cgatggcctg ccaggcggcc ttgtagctca cgcctgcgcg acgggccgct
    29341 tcggagatgg agccgacgtc gccgatgcgc ctcaggatgt caatgcgctt gtcggcgagc
    29401 tccgccatgt gagtcgccaa aagcgtcggg tcggcaatcg ttttgctcat gcttgtctcc
    29461 gaatggacta cttgaaaaat gaggaaagtc ccgctattct tctacggaat agcggagccg
    29521 tcaaggccta cactcaatct tagcttaatg cggttcaaca ccgcccatgg agaaacaatc
    29581 atgcagatga agaagcttgc cgccgccctc atgctcgcag gccttgcctg cgctgttgcc
    29641 gccgaagaag tccgcgtcgc cgttgccgcc aacttcatgg ctccgatgaa ggagcttgct
    29701 ccgctttatg aaaaggccac gggcgacaag ctcgtcgtgt cctacggcgc cacgggcgcc
    29761 ttctacgcac agatcaagaa cggcgccccg ttcgacgtgt tactcgccgc cgacgccaag
    29821 acacccgcca aggccgtcaa ggaaggccac ggcgttgccg gcaccacctt cacgtatgcc
    29881 gtgggcaagc tcgtcctctg gtcctccgac gccgcactcg tcaaggacca gaaggccctt
    29941 ctccatcccg ccgtgaagaa ggtcgccgtc gcagacgctc gtctcgctcc gtacggcgag
    30001 gccgcccttc aggcgctcga cgctctcaag atgaaggagg ccgtcgagcc gaagttcgtc
    30061 gtcagcaaca acatcggcaa gaccttccag ttcgtggagt cgggcaacac ccagatcggc
    30121 ttcgttgcgc tttcccagtg cttcaagaac ggcgccttca cgggcggctc cggctgggtc
    30181 gttccgcagg acctctacaa gcccatcctt caggatgccg tccagctcaa ggcaggcgag
    30241 aagaacgccg gcgccaagcg cttcctcgac tacctgaaga cgagcaatga agccgccgcc
    30301 atccgcgaag cctatggcta cggcacggtc aggtaagtca tgctgacatg gactgaactc
    30361 ggacccgtgt ggctgagcct gaggctcgcg gccgtcacga ccgtcattct cctcatcatc
    30421 ggactgccgc ttgcgtggtg gctcgtgaaa ggcaggagcg cgctctgcgc gccgatgaat
    30481 gcggtcgtga ccctgcctct ggtgttgccg ccgtccgtcc tgggcttcta catccttatc
    30541 gccctcgggc ccaacgggcc gatcgggcag gtgacggatg ccctggggct gggaacgctc
    30601 aacttcacct tcaccggtct cgtgatcggt tcggtggtgt attcgcttcc cttcatgatc
    30661 cagcccgtcg tcacggcctt cgaggccata ggcccaaggc cgatggaggt ggcggcaacg
    30721 ctcggctgcc gccccttcga agccttcttc cgcgtgatcc tgccgctggc gcgccacggc
    30781 atcatcacgg gcaccctgat gacttttgca cacacgatcg gcgaattcgg cgtcgtgctc
    30841 atgatcggcg gcaacatccc tggatcgacg caggtcatct caaccgaaat ctacacgtac
    30901 gttgaagcga tggagtacgg caaggcgcac gttcttgcgg gcggcatgct ggtcttcagc
    30961 ttcttcgttc ttctgtccct caacttgctc aaccgcaagg aagggaggac ccgatgagcg
    31021 gcaggatcac attgaatctg gaccgcggaa cggacttccg ccttgacgtt tcgttcgaga
    31081 ttcccgacaa gggcatcacg gtgctcttcg gaccctcggg ctgcggcaag accaccgtgc
    31141 tgcgatgcac ggcgggcctt gagcgtgcgg gcggcagggt cgtcgtcgcc gaccgagtct
    31201 ggcaggacga tgccaggaat atctttgtcc cggcctggga gcgccgcttg ggatacgtct
    31261 ttcaggaagc gagtttattc gagcatctga atgtcgaggg caatctccat tttggcctca
    31321 gacacaagga tgcccgtggg gggctcgccc gcttctcgga agcagtggag ctcctgggca
    31381 tcgggcatct tctccggcgc cgcgtgcagc ttctctccgg cggcgaacgc cagagagtgg
    31441 ccatcgcgcg tgcgctgctg atgacgcccg atctcattct catggacgag ccgctcgcgg
    31501 cgctcgactg gaaccgcaag caggagatcc tgccgtggct cgagcgtctt cgcgacgagc
    31561 tctccgttcc catgctctac gttactcact cggccgacga aatggcccgc cttgcggatc
    31621 aggtgatctg ctttgaaaac gggcggatcg tcgccgaggg gccgcttgag gaggtgcttc
    31681 tcgagcgctg gcccggacag ggcgagcagg gctcctccgt cgtcctttcg ggcgtcgtga
    31741 ccgagcggag cgaaatgtgg tgcacggcgg tcgtgacggc gggtgagacg gccttcgaag
    31801 tgcccgatgc gggcaggaac gtgggcgagg ccgcgcgcct gaggattctt gcgcgcgacg
    31861 tgagcgtggc gctcagcgaa ccgagggaca cctcgatccg aaacgtgctg cccgccgtcg
    31921 tcatcgggat gcgaggcgtt gagggcgaga gccacgtcat gctcacgctc gaggcgggcg
    31981 gttgccgcat tctctcgcga atcacccgtc gctcggcgga cgaactcggc atcgccgtcg
    32041 gcacgcgcct ctatgcgcag gtgaaggctg cggccatcat cgtctagcgc ccgcagggcg
    32101 ccccgagggg tgcttttgtc gccaaaagaa cgcccgctat atgcaacccc ctaatcggtt
    32161 ggcgaatcct caagaggcgt tcacttaaat ttgaaggccc cgcttcttct tgcaagaatg
    32221 tgcgctgcga cgatgtcgca acgtacattt ttcttaggag tagcaacgtg ccctgccatt
    32281 ttctgaagct cagcgacttt tccggtgaaa cccttcgcta catgatcgac cgcgccaagg
    32341 tcatcaaggc ggacctgaag gcgggcaagc gatatgaacc gttcgcaggc aaggtgctca
    32401 tcatggtgtt cgaaaaggcg agcacgcgta cccgcgttag cttcgagacg ggcttctacc
    32461 agcttggtgg caacgtcgtc aatctgcaga gccagggctc ccagatcggc cgctccgagt
    32521 ccatcgccga cacggcccgc gtgatcagcc gcatgggcga cctgatcatg atgcgcacct
    32581 tcggccagga ccgtcttgac gatatggccg ttcacagtcg ggtgcccgtc atcaacggtc
    32641 tcacgaacga ataccatccc tgccagatcc tcaccgacat cctcaccttc gaggagcact
    32701 gcggtccgat caccggcaag accgtcgcct gggtgggcga cgccaacaac atggcctaca
    32761 cctggatcga ggccgccaag ctgctcggct tcaagctcca cttctcgagc cccaagggct
    32821 atgagcttga tcccgccctg acgcttgcgg gcgaccatct caaggtcttc gacgatcccg
    32881 tcgaatgctg ccgcggcgcc gatctcgtga cgaccgacgt ctggacgtcc atgggctacg
    32941 aggaggagaa cgcggtgcgc cgcgaggcct tcgccaagtg gcaggtcaac gccgactcca
    33001 tggccgccgc caatcagggc gccgtcttca tgcactgcct gcccgcccac cgcggcgagg
    33061 aagtcacggc cgacgtcatc gatggtccgc agtccgtcgt ctgggacgag gcagaaaacc
    33121 gcctgcatgc ccagaaggcc atcatggaat actgcctgct cggccgtgtc gaataagaac
    33181 cctcgaacga cctgagagac agcgcgcaag gccccgttca atcgagcggg gctttcgcgt
    33241 tcttgtcgga tgggcgcgca tgaaaagaaa agccccgctc aatgacgagc ggggcttttg
    33301 cctgaggatt tagagccgtc cgaagggacg gtcccttggc aggcggatct caagcggatc
    33361 agcagcggcg ggcgagttcg acggccttgc cgatgtaggt ggcgggcgtg agttccatca
    33421 gggaggcctt ggcatcggcg gggatgtcga ggttggagat gaattcgcgc atcgtttcct
    33481 tggtgatgcc gtccttgccg cgggtgaggg ccttgagctg ctcgtacgga tgcggcacac
    33541 cgtagcggcg catcacggtc tgaacggctt cggcgaggac ttcccaggcg ttgtcgaggt
    33601 cggcggcgat gacctgctcg ttgacctgga gcttgccgag gccgcgcgtc agggcgttgt
    33661 agccgatgaa gcagtagccg aaggcgacgc cgaggttgcg caggacggtg gagtccgtca
    33721 ggtcgcgctg ccagcgggag atcgggagct tgccggcgag atgatcgaga acggcgttgg
    33781 cgaggccgag attgccttcg gagttttcga agtcgatcgg gttgaccttg tgcggcatcg
    33841 tggaggagcc gacttcgcct tcacgcagct tctgcttgaa gaagtgcatg gagatgtagc
    33901 cccagacgtc gcggtcgaag tcgatgagga tcgtgttggc gcgttcgatc tggtggaaga
    33961 gctcggccat gtagtcatgc ggctcgatct ggatcgtgtg cgtgttgaac gtcacgccga
    34021 ggcgttcctc gacgactttg cgggagtggg cttcccagtc gtattccgga taggagacga
    34081 ggtgggcgtt gtagttgccg acggcgccgt tcatcttggc gtagatcttg acggcacgga
    34141 tggcttcgat cacgcgttca agacgcacgg cgacgttggc gaattccttg ccgaccgtcg
    34201 tcggagaggc ggtctggccg tgcgtgcggg agagcatcgg gatttcggcc cactggtggg
    34261 cgaagcctgc gatcgtgtca tggatctgct ggaggtagtc ggcgagggct tcgcggccgc
    34321 gcttgagcat gatggcgtgg ctcgtgttgt tgatgtcctc ggacgtgcaa gagaagtgca
    34381 caaactcgga ggcggacttg agctcttcgt cgtgggcgac ctggttcttg atccagtact
    34441 cgacggcctt cacgtcatgg ttcgtcgtct tttcgatttc cttgatggct tcgcagtcgg
    34501 cgagcgagaa gttgtcggcg aggccgcgca ggaaggcctt gccgtggtcg ctgaggtgcg
    34561 gaagttcagc aaagccggct tcggaaagac tgatgagcca ctccacttcg acacgaacgc
    34621 gggcgttcat gaaggcgcat tcggagaaga tgtcgcgaag cgcatcggtc tggcgggcat
    34681 agcggccgtc gatcggggag agggcggtaa ggggattcag ttccatgtcg gaaaacctcg
    34741 ggtgaaaaaa gaaaaaacgg cgtccgctgc gcaggagggg ccgcagggag cggcgggcgg
    34801 aacggacaaa agacaatcct tgtattttag cagtccgttt cgcgtgcgtc ttcccgacgg
    34861 gccgtcttcg gcacgggggc accgtgcagg gaaaagtgtc ctcagcaggc gtgttcctgc
    34921 gggatgacgc ctctttttgc cctacaatcg gacttgcgcg ttcggggtat cggcctttca
    34981 ggggccggtt gagaaacacc cgtcgcaccg gatcaggttc agacctgcgt cgggaaacgc
    35041 tgcggacaaa tcgacatccg gccttcgaag gaaggcccac ggcaccgttc tcttcgccgg
    35101 tcgcgccctc acttcaattc ttctggagcg cgattatgca caaccacttc cattccaatc
    35161 ccgacctgcg ccccgctggc gaacgcttct tcgtgaggag cgaggcgggc gaggacgtcc
    35221 ctctgcgccg catcaggctt tccgacacgc ccgaatgctt cggcggcggc aggaatcccg
    35281 acttcttcat gaccgacgtc acgggcggct acggcgccgc cgatgcgggc gagacggtcg
    35341 agctcgtgcg ccgcgagcgc cccgaacctg gcagccccac gcagctcgcc gccgcgcgag
    35401 cgggcgtcgt gacgcctgaa atggcctatg cggccgcacg cgaaaacgcc aatctcgaag
    35461 gtttcgccgc ctcgctctcc gagccgggac ttgagcgcgt gcgcgacagg ctcatgccgc
    35521 ttctcgaacg cgtcgagcct tggacgggcg agcgcgtcag ggcccgcgtg gctgcccgac
    35581 aggccgtcat ccccgccgcc ttcacgcatc ccgaggtcga gcccaccgtc atcggccgcc
    35641 acttcgccac caaggtcaac gccaatgtcg gcacttccgc ctcgtcctcc gactggcgcg
    35701 aggaaggccg caagatgcag gaggcgctcg ccagaggcgc ggacaccgtg atggacctct
    35761 cgaccggcag gtgcatccgc gagacccgcg agatgatcct gcgcggctcg cccgtgcccg
    35821 tcggcaccgt gccgctttac gaaacgctcg agcgcgcagg cggcgatccg ctcgcgctct
    35881 cctgggagat cttcagggac gtcctggagg accaggccag ggcgggcgtc gactacatga
    35941 cgatccacgc agggctcctc ctgccccacg tcgagcttac gcgcagccgc ctgacgggca
    36001 tcgtctcccg aggaggcggc atgatggcca ggtggatgaa ggccttcggc agggagaact
    36061 tcctctacga gcactttgac gagatcctcg acattgcggc gcactacgac ctgacgctct
    36121 cgctcggcga cggcctgcgc tcgggcagcc tctacgacgg caacgatgcg gcgcagttcg
    36181 ccgaactcag gacgctcggg gagctcaacc gcagggcggg tgctcgcgac gtgcaagtga
    36241 tgatcgaggg gcccggccac attccgatgc ccggcgtcga gctcaaccag ctcaatgagc
    36301 gcgagtggtg cgatgacgcg cccttctaca cgctcgggcc gctcgtgagc gacgtcggtg
    36361 cgggctacga ccacatcacg gccgccatcg gcggcgccat gatcgcgggg gcgggcacgg
    36421 cgatgctctg ctacgtgacg cccaaggagc atctcggcct tcccgatgcg aacgacgtgc
    36481 gcgagggcat ggccgcattc cgcatcgcgg cccatgcggc cgacatcgcc cgaggcgtgc
    36541 ccggtgcgat cctgcgcgac cacctcatga gcatggcccg ctttgaattc cgctggcccg
    36601 accagttcgc ccttgccgtg gatcccgcca gggcccgtgc cttccatgac gagacgcttc
    36661 ccggggccgc cgcccagaag gctcacttct gcagcatgtg cggcccgaag ttctgtccga
    36721 tgaagctcgc gcacgagctc ttcaagaatt gatcggagcg gggcagccgc gaatgaaaaa
    36781 aaaa
//
LOCUS       DBMDTI010000004        33756 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_517_length_33756_cov_3.826326, whole genome shotgun sequence.
ACCESSION   DBMDTI010000004 DBMDTI010000000
VERSION     DBMDTI010000004.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 33756)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 33756)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33756
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_517_length_33756_cov_3.826326"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="ACZPB6_00640"
     CDS             <1..332
                     /locus_tag="ACZPB6_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR1183019.1"
                     /translation="GKVTVTRHPDGCLVLYPAPVWEEKRRALAKLPYSARAFVRYVLG
                     SAVDLEPDRAGRILIPSELRQMCGLDREVALIGMGEHFELWDRSTLEAREREALAAEL
                     DAADFSF"
     gene            394..1365
                     /gene="rsmH"
                     /locus_tag="ACZPB6_00645"
     CDS             394..1365
                     /gene="rsmH"
                     /locus_tag="ACZPB6_00645"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR1183020.1"
                     /translation="MSEAFIHLSVLGEEAPEALVGDPDGLYVDGTFGRGGHSRRILEK
                     LSPKGRLIAFDRDPQAVEVARSWTDPRFTIIHEPFSTMTESLAAIGVHQADGIFLDIG
                     VSSPQIDDAARGFSFRFDGPLDMRMDTTRGMTAAEWLAQAEERDIRHVISLYGEERFA
                     GPIARAIVARRKTKPLETTRELADLVAGVVPRNRKDMGQHPATRTFQAVRIEVNHELD
                     ELKQALDAAGGLLKPEGRLAVITFHSLEDRIVKHFFDRAAHPERGLDPRLPLTNDMLP
                     KPLFDNIERIKPSEAECEANPRARSAILRVGVRTVSPWRPEDGACAR"
     gene            1353..1736
                     /locus_tag="ACZPB6_00650"
     CDS             1353..1736
                     /locus_tag="ACZPB6_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183021.1"
                     /translation="MRQVSKMDLVVVAVLGLVLFALAITLVNVQYRARTLFVEHERAV
                     DVGRRLADDEAHLLLKVRKASLPGSIADGAGALGLEVARGENTVNLVVDEHHRVTFAP
                     DTAAAQAAAADGKAGASEGSRGGRP"
     gene            1733..3568
                     /locus_tag="ACZPB6_00655"
     CDS             1733..3568
                     /locus_tag="ACZPB6_00655"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1183022.1"
                     /translation="MKLSLLEWIERSVLAVWEKIADFLAKDREARKAAPEHAADPLII
                     IPIPKNRTYLAFFGILMLFFILAGRTFYLQVFNNDFLQNEGEKRFARTITIQGPRGEI
                     LDRNGIVLASSQPCRSVWADPALVPRSTKEEKQQLRELAKLLGIKYSELLKKLDHSSS
                     PNFVYLARNRDLGLAEAVGALRIPGIGMSPEMRRRYPDGPAMAHIVGYTGADDHGQEG
                     VELARDHVLAGRAGSRRVIRDRHGRIVDDVWAKESEAGTDVTLAIDSRVQFIAYEALR
                     AAIERHQAKAGAVVVADVNTGEIIALANEPTYDPNDRSTVLFERVRNRVLTDQFEPGS
                     TMKPFAIAKALDMGLVDPLTTIQTSPGKLTIGDRTIGDTHDYGLLTVAEVVAKSSNIG
                     TAKIALEISPQTLYETYSELGFGNAPKIGFPGATSGRLRPGKTWRPVEQATISYGHGV
                     TVSLMQLVKAYTALARNGDVIDLTLFKRMPGETAAGTQVFKPETARRMRAMMMATVKR
                     GGTASNVNVAGYSVAGKTGTANKVKNGQYTRDVVASFVGIVPATQPRFIVAVMVDEPK
                     KGRFGGLVAAPVFNEVATGALRTLMVSPDDKTAPAGGGLLAKVRH"
     gene            3592..5103
                     /locus_tag="ACZPB6_00660"
     CDS             3592..5103
                     /locus_tag="ACZPB6_00660"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR1183023.1"
                     /translation="MNKSMNEVTAWLKALVHDGAHLRLDSRSVKPGDVFVAVPGLKAD
                     GRAFIRVAAARGAAGVLMETRNDAPDAERFPIASLSVDDLSKHLGEVAAAFYGDPSSR
                     MTGVAVTGTNGKTTTTHWIAALLTNFGTPCAVMGTVGCRFAGESFDMPALTTPDAVSL
                     QGVYADLEKAGAKAFAVEASSIGLEQGRMTGSHFDTAVFTNLTRDHLDYHVTMDAYAE
                     AKAILFAWPGLRTAVVNVDDPYAGLMAETARKNGAAVWATTLTGVVPFAADHVVEASD
                     IEATEAGTRFLMKVDGAATSVHLAQVGGFNVSNFIQAATVLLAKGCEADRLAAWAESL
                     ASPPGRMQLMRAEGAPLAVVDYSHTPDALEKALESLRPVAQARGGRLWAVFGCGGDRD
                     AGKRPVMGGIAVRLADEVVVTSDNPRTEDPDAIIADISAGAPGARVVTDRAEAIHHAV
                     AEADGADVVLIAGKGHEDYQEINGVKHHFSDSEVVREAFNERRVQMLRAVEEV"
     gene            5106..6458
                     /locus_tag="ACZPB6_00665"
     CDS             5106..6458
                     /locus_tag="ACZPB6_00665"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR1183024.1"
                     /translation="MECLQQLLEALEPVVVAAAGDFSQSYEGFSTDTRTVKAGNVFVA
                     LSGERFDGHDFVVGAAEAGAVCAIVERKVDAAITQIVVTDSGLAFGLAAKAWRGRFAL
                     PLAVVAGSNGKTTTTQMLASILLARWGAERMCATEGNFNNEVGVPKMLLKLAFEHRGA
                     VIECGMNHRGEMARLADWTRPTVALLTNAQREHQEFLRGVEETARENGLVIAALPESG
                     TAVYPADDACADIWADLALARGVDELTYSTDPDVEADVMGRVENGELVISTDEGDVVR
                     TKLRITGSHNVHNATGAAAAAFAMGIGPEAVAAGLGAFEPVPGRGTRFVGKHLVVIDE
                     AYNANPDSVRAAMNVLAGEAGPRTFILGDMGEIGDSAEEAHREAGEYARSLGIDALYA
                     CGPLSRHAAEAFGEGGRWFETRDALIEALKGLRGTATVKASHFMGFAAVVRALQDNEE
                     "
     gene            6508..7662
                     /gene="mraY"
                     /locus_tag="ACZPB6_00670"
     CDS             6508..7662
                     /gene="mraY"
                     /locus_tag="ACZPB6_00670"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR1183025.1"
                     /translation="MLLELFRSLADGFPALNVFNYLTLRAVLAALTSMAIGFALGPWM
                     IARLKNLHFGQAVRTDGPKTHLVKDGTPTMGGALILFAITVSTLLWADLSNRFIWVVL
                     FVTLGYGSIGWIDDYRKVVHHNPKGMSAREKFSWQSVIGLAAAFYLVFALSVPAGGTL
                     TETIAGWVEAGFPLDISSNLHLLIPCFKDIALPLGLVGFMVFTYVVIVGSSNAVNLTD
                     GLDGLAILPSVMVGGALGIFAYVVGRPDYAGYLIFPYIPGAGELVVIAGGLLGAGLAF
                     LWYNAHPAQVFMGDVGALALGGCLGTMAVITRQELVLAVMGGIFVVETLSVMIQTVYF
                     RLSHGKRIFLMAPVHHHFELKGWKETQVVTRFWIITMILVLIGLSTLKIR"
     gene            7705..9147
                     /gene="murD"
                     /locus_tag="ACZPB6_00675"
     CDS             7705..9147
                     /gene="murD"
                     /locus_tag="ACZPB6_00675"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR1183026.1"
                     /translation="MKQEVDKKTALIVGLGASGLACAHHLGRRGWKIRAADTREAPAG
                     AQRLGAEMPDAEIRTGGLPESLLDGVSLVVMSPGLSTRFGAAAPLVASARARDIEVVG
                     EIELFARALADLEARTGYRPVVIGVTGTNGKTTTTTLVTKMAAEGGRRATAAGNIGPN
                     AVTELDKALDAGVLPEVWVLELSSFQLDTTSSLKCVSAAVLNVTEDHLDWHGDMKAYA
                     EAKGRIFKQDTVRVVNREDAETLRLAQGGPVRTFGGGEPVKAGEYGLARGADGLLWLS
                     RLDEGASEPTLFCPEKSLLIAGRHNAMNALAALALIEAAGLPVGPALAALAAYGGEPH
                     RVERVLDCDGVTFIDDSKGTNVGAVAAAVKGFAAQGRRILIILGGDGKGQDFSPLVEA
                     LKGTAGAVALIGRDAPLIEEALSATGIPMARQTTLEEAVYWLWARHAQGDVLLLSPAC
                     ASWDMFRDYAERSARFIACARAIAAEEQAC"
     gene            9141..10346
                     /gene="ftsW"
                     /locus_tag="ACZPB6_00680"
     CDS             9141..10346
                     /gene="ftsW"
                     /locus_tag="ACZPB6_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR1183027.1"
                     /translation="MLSFGRKKKKEDWQTQGRAVATLKERANASLEVIICVAALLGIG
                     TVMVYSATSVFADNSRYGVDALYFVMKHLQSIVVGVCAGWIVTKIPMSFWQRYALWVF
                     FGALILLVCVLLPGVGKTVNGAKRWISLGVMNLQVSEIMKVAALIMAAWFAVMRQEYM
                     HSFQKGFIPMAFAMVAIAALIIQQPDLGAMVVIVAEIMGVLFLGGLSFKIFTFVISVV
                     VGFVFWMIIDTPWRLGRIFAYWDPWAPEHVLDKAYQLSHSLIAFGRGELFGVGLGGSV
                     EKLYYLPEAHTDFIMAVIAEETGFVGVCLVLFIYYWLIRHAFEIGRVSIKLERFFQGL
                     LAQGIGVWFGVQVFINVGVASGLLPTKGLTLPFVSFGGSALLSGLIAIGLLLRVDKEN
                     RMLMRGGQV"
     gene            10343..11455
                     /gene="murG"
                     /locus_tag="ACZPB6_00685"
     CDS             10343..11455
                     /gene="murG"
                     /locus_tag="ACZPB6_00685"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR1183028.1"
                     /translation="MSTDSKHLVILAAGTGGHVMPGLAVAKVMASRGWKISWVGTVAG
                     MERGLVERHGIPFHALDFQGVRGRGPLGAIKGGFKLIGAFFASMKLLNELKPDAVFST
                     GGYVAVPFGFAAHRRKLPLVMMNCDADLLMSTNTLLPFCDALACGFAGGARTFAKEKG
                     RTTGNPVREEIVAVSPPQDRMANRTGPLRVFIFGGSLGAQVFNDNMPAVFAAIPQEQR
                     PIIVHQTGKDRDAPVREAYEKLGVKAEVVPFINDMAARYEDCDVVICRAGATSVSELC
                     AAGVASILVPFVAKTTAHQTGNAKYMVERHAAIMVPQPEFTVERMAELISGLTRDHLL
                     ELAKNARATARPQAAEAVANLIVETVARRTGAQDAK"
     gene            11504..12904
                     /gene="murC"
                     /locus_tag="ACZPB6_00690"
     CDS             11504..12904
                     /gene="murC"
                     /locus_tag="ACZPB6_00690"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR1183029.1"
                     /translation="MKFLVNHLHFVGIGGAGMSGIAEVMLNLGYVVSGSDLSKSAVTE
                     RLQSLGARVFCGHDKKHIRGADAVVISSAVHEDNPEVVAARELGIPVVPRAVMLAELM
                     RLRRGIAIAGAHGKTTTTSLTASLLAAGDLDPTFVIGGRLNSAGANARLGTGEFLVAE
                     ADESDASFLNLTPVISAVTNIDEDHMDTYGHSFENLKSAFVDFLERLPFYGVAVLCAD
                     DEHVLEIRPRVTKPVILYGLSADAQVRAIDVRAVGTQMAFTILRPDHPPLPVVLNLPG
                     IHNVRNALAAVAIATVVGVSDEAIVRGLSEFRGVGRRFTQYGDIPVRAADGSVTGHYK
                     LIDDYGHHPHEMAATLAAVRGVWPEKRVIVAFQPHRYTRTRDCFEQFVDVLEDIDGLV
                     LAEVYPAGEALIEGADSEHLAAAIAAKGFHRPVVVPVEGVADAIAGMVRDGDVVITMG
                     AGSIGRVAPAIADGAK"
     gene            12957..13889
                     /locus_tag="ACZPB6_00695"
     CDS             12957..13889
                     /locus_tag="ACZPB6_00695"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR1183030.1"
                     /translation="MENLGRVVLLMGGVSSEREVSLMSGEGVHKALVSKGVDVLKFDP
                     KTDSLETLEKGGFDRAFIALHGRLGEDGTIQGVLNYLGIPYTGPGVTASAMAIDKDLT
                     KRVWRSAGIPVPEGVMIDPKADDAVLEEAIACLGATGLVVKPAHDGSSIGVTKLAEPT
                     VAALRKALEDAAGVRGDEVLVEEYIHGREFTVAVLDGRALPVIEIRAPEGDYDFNNKY
                     FGDAVHYDCPAKLEDEAAATLGSLCERAFAAVGARGWSRIDALQRADGSFALLEINTS
                     PGMTPHSLVPMAARAVGMDYADLCLKVLSLARCD"
     gene            13924..14865
                     /locus_tag="ACZPB6_00700"
     CDS             13924..14865
                     /locus_tag="ACZPB6_00700"
                     /inference="COORDINATES: protein motif:HMM:NF015739.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR1183031.1"
                     /translation="MKAESPENPAEAVTPEEAIERRALARAQEEAREARRASLRFWTK
                     TVCGLAAAAVVVLGAGRAADAVMHLPVFDFSTLEVEGDVARVPVERVKQAVEPVIRGN
                     FFTEDLDEVRRAAETVPWVKRAVVRRVWPDGLRIAVETYSAFAVYEDGRLVDPDGVLF
                     SANPDERDNPSDPLPNFYGPAGQTAQIARYYREFSRALSSIGATVTDVACSDRGGWSL
                     VMASGDIPATRVELGREDAGTNIVAKVADLAAAYPRVVELMDGPPASIDLRYDRAFAA
                     TLPDREAIRKLREAEQRTDTDVPDEEPGSAQHDEQPE"
     gene            14849..16081
                     /gene="ftsA"
                     /locus_tag="ACZPB6_00705"
     CDS             14849..16081
                     /gene="ftsA"
                     /locus_tag="ACZPB6_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR1183032.1"
                     /translation="MNNPNDYLVGLDIGSHKVLCVVALPSPKGEGLYRICGYSFRDSH
                     GVRNGIVTDLNAAVDDIKAAVREARSAGNLPELSNAWVAIGGSSLTSENCLGTAVVRG
                     NEVKPADVEAAENNAREHSLRQGKRLIKMIPQGYSCGDTFSQTPVGLVGDKVTAYYHA
                     VYGSVKNAENMKRSLLRSGIELAGYEPHPIAAALAVSTESDRYNGTLVLDMGAETTSM
                     TLVYEHRTLLTAVRPFGSEFFTRDLSTIFGVSLDQAEELKIRFGSCSMDGVLPGESVR
                     PSLEDVRGTPLCSRSLIVQTLYERAKEFFRIYRDVIEKAGHLDKVHAVIITGGGANLR
                     AIDEVARDVFGVPVRIGAPLCFDEKNALSMRPNASVAAGLLMAADKTRAAGDSRSYRT
                     PSLLNLSGRIRTVFFGDY"
     gene            16174..17319
                     /gene="ftsZ"
                     /locus_tag="ACZPB6_00710"
     CDS             16174..17319
                     /gene="ftsZ"
                     /locus_tag="ACZPB6_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR1183033.1"
                     /translation="MPFEILQELDPLNTVIKVIGVGGGGGNAVEHMITHGAKGIEFIA
                     ANTDHQALSRSRAHVNIQLGSTGLGAGARPEVGAAAAQEKREQVAEAIRGAHLLFITA
                     GMGGGTGTGAAPVIAEIAKEMGILTVAVVTKPFSFEGGRRMRCAEKGIENLKDKVDSM
                     IVILNEKLESECPPNATMKECFEMSNEVLYKACVGIAEIIHTPGTINVDFEDLKTVMS
                     ERGTAIIGLASASGPDRARVAAEKAIACPLLEGANLQGARGLLAYFTGNESVTLAEIK
                     EAMAVLNTFVHKSAIVIFGSAISEEMGDEIRVTLVATGLDRPLDANEPGSGVQAPAVF
                     NASSEAPAGLQTPVFGQTLEAPAPEPAAQQGDAAVDVSKFVIPTYLK"
     gene            17402..18322
                     /gene="lpxC"
                     /locus_tag="ACZPB6_00715"
     CDS             17402..18322
                     /gene="lpxC"
                     /locus_tag="ACZPB6_00715"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR1183034.1"
                     /translation="MFKQRTLRQSVETVGIGLHSGRKVHLVLRPAEPDTGILYRRVDI
                     RPAVEIPAHPLNVNDTRMATTVNVGKAAVATIEHLMSAFNGLGIDNVIIEVDSAEIPI
                     MDGSGASFVFLLHQAGIVEQDAPKRFVRVLKPVEVREGDKLARLEPHEGFRLTFTIAF
                     GHPAIDRTEQKADVDFSRVTYEQAVSRARTFGFVQDVEMLRRMGLAQGGSLENAIVMD
                     EFRILNPGGLRENDEFVKHKILDAMGDLYVLGHPLLAHYVAHKSGHGLNNQLLRALLA
                     DETAWEWATFDKAEDCPVDFWTQKTDQVRP"
     gene            complement(18307..19446)
                     /locus_tag="ACZPB6_00720"
     CDS             complement(18307..19446)
                     /locus_tag="ACZPB6_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183035.1"
                     /translation="MTQDFIRRRIGRQTIDSCLARDELQPLMASYALSKRAGAVAAHV
                     LRPFRMSIDFTDPAYCRVEGHTLVLLAESAVQQNRIRQLTPRLAAAAAAAGLPVTAVD
                     ARIIPKRPAPQAGVPIPRIERAPSAVGAAAISGILDDIEDPALKATMERLRNVIKPGE
                     ENRRRLLEERLGRESIDLSTERMRLTTLVADLTHGINAAIIPTEEEARDYPRLEAERE
                     RKLELLAEQKDRRLVAVHRLERIDARLDAINRALELLPSDAQAAEAALVAPEDEEAPQ
                     APPAAPRVSAAAAAAIASLARETPSPRMRKALMSHIPTLAPDEAHYPEALHADIRREA
                     ERLSMLENPTPENTKRLAQLLEAAKALSERPQDARAIAEGLYGLT"
     gene            19705..22401
                     /gene="secA"
                     /locus_tag="ACZPB6_00725"
     CDS             19705..22401
                     /gene="secA"
                     /locus_tag="ACZPB6_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR1183036.1"
                     /translation="MFDELLTKIFGSRNDRLIKQYRRKCDAINKLEPAMQALSDEELK
                     AKTQEFRDRIAKGATTDELLPEAFAVVREASVRVLGMRHFDVQLIGAMVLNDGKIAEM
                     RTGEGKTLTATLAVYLNALPGKGCHVVTVNDYLASRDADWMGRLYNWLGLSVGKILSN
                     QNNEEKRAAYASDITYGTNNEFGFDYLRDNMEYDVAARRQRPLYYAIVDEVDSILIDE
                     ARTPLIISGPADDNTELYRAVNDIPPLLTRQAEEKGEGDYWVDEKAHQVYLSEAGHEH
                     LEQILSERGLVQQGESLYAPKNIILMHHLNAALRAHTLFHRDQHYVVQNGEIVIVDEF
                     TGRLMPGRRWSEGLHQAVEAKEGVKIQQENQTMASITFQNFFRMYGKLSGMTGTADTE
                     AYEFQDIYGLETVVVPTHRKMVRIDEQDKVYRTVNEKYRAIIDDVKACYERRQPVLLG
                     TTSIENSELLSSMLTKEGIPHNVLNAKQHDREAQIVLEAGRPGVVTIATNMAGRGTDI
                     VLGGGINKAVDAIRADETLTEEQREAKIAEVKAEWQKLHDIVVEAGGLRIIGSERHES
                     RRIDNQLRGRAGRQGDPGSSCFYLSMEDQLLRIFGGDRMRAIADKLRLEEGEAIESKM
                     LTRMIESAQRKVEGRNYDIRKQLLEFDDVQNDQRHEIYGLRNQILESEDISELVKNLR
                     EGLFTDLFRAYVPAETVEEQWDLDGLQTKLAGEFGIVIDFKQLLDSNDQFTDDDLLEA
                     LLKQAEAQYVEKEQMVGHEAFTGFARSILLQILDQLWRQHISALEALRQGIYLRGYAQ
                     KQPKQEYKREAFNMFEQLLDQIRETLVRVLMQVQINVKPAEEPAQQQPVEEPAPAEAT
                     GSALENMPKEELDPALFAHVGRNDPCPCGSGRRFKDCHGRLS"
     gene            22506..23576
                     /locus_tag="ACZPB6_00730"
     CDS             22506..23576
                     /locus_tag="ACZPB6_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183037.1"
                     /translation="MRNERSISLPGLLLGFSVDGGVAAAAVPAAARTVLSLPQVALGD
                     GTAQALRMRRMSGSRTGCILMSRGYPMFGAGGLSDAGLSAFRHEALRRLELPEDALAL
                     LLQGTRRELTPVGGLVERCTEAWKRAGGEALLPEGWTGFEMPFMTQDGVMRVGALSAG
                     GAVPTENRVLLLVTDVPVREVILQRIADRAAGPLFAWLRAVGVLSAADVVMVVSAAGR
                     RPVASIDDPAAEMLARALELAVGRVSEAWAGALGRNIRVRLEGLRDEEEAEHLIAALA
                     GVGGWLRGAGTPREVSLVLRAALEQSALPGLEASGFAVRLGECADALSLASAQELSNL
                     QEGRAAITLFMARGNLSTTFCV"
     gene            23657..24337
                     /locus_tag="ACZPB6_00735"
     CDS             23657..24337
                     /locus_tag="ACZPB6_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR1183038.1"
                     /translation="MSEDKVVDVAVGVLIREDGRVLLASRPEGKPWAGYWEFPGGKLE
                     DGETVHQALVRELDEELGVRLADSFPWFVKEHRYEHAHVRLHFRRSRDFFGEALSKEG
                     QDFGFYAANERTPGVLLPIDQNILNRVDLPDVWEDSTAVLTLSENALHATVVRDRKYR
                     FVGARCGNLDDVMKAVAMDFDFVIVKPEVFEATLWKGEPRLPTYVEDVPASDLRLWQD
                     RGAHGVKP"
     gene            complement(24434..24667)
                     /locus_tag="ACZPB6_00740"
     CDS             complement(24434..24667)
                     /locus_tag="ACZPB6_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR1183039.1"
                     /translation="MKVKCPTCGKEIEYSSANPWRPFCSERCKLIDLGEWASDGYVIE
                     GDPGSADRMTPEELESVARYTAEREERKGGRRR"
     gene            complement(24680..25450)
                     /gene="zapD"
                     /locus_tag="ACZPB6_00745"
     CDS             complement(24680..25450)
                     /gene="zapD"
                     /locus_tag="ACZPB6_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HIR1183040.1"
                     /translation="MSTAEHLLYEFPCNEKIRTYLRLEALFQRFDWFCEQTSAIAHQA
                     GISALFDLLDATARSDLRHELIQELERQRQRLIAKNASADPDMAAHLAEIGAAIQAVS
                     DSVGRTTQSVRENQWLQIIRTRQSIAGGTCEFDLPQMHLWMNTAPEIRRRELQSYVST
                     LEPIRTGTMLLLRIFRANSHCQSLTAVNGTYQFSMNRNQNCALVQIWLPADSTVVPEV
                     SANKYMLWIRFSRPDENHKLHLARTEEIPFRLGLCTPS"
     gene            complement(25522..26124)
                     /gene="coaE"
                     /locus_tag="ACZPB6_00750"
     CDS             complement(25522..26124)
                     /gene="coaE"
                     /locus_tag="ACZPB6_00750"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR1183041.1"
                     /translation="MSLIIGLTGGIGAGKSTASAHLRASGLPVVDADAISRALTGPGA
                     AGSLAVAEAFGPEFLTPEGAMDRRRMRELAFRDAGALKRLEACLHPLIGAAVTREFES
                     LSASPIVIYDCPLLWRDDFRHPAVSRVLVIDASDDVRLSRIVSRPGLTEETARLMMKA
                     QPPRSRILQCADDLIVNEDDEAALRKRLDALSRIWMAITR"
     gene            complement(26187..26263)
                     /locus_tag="ACZPB6_00755"
     tRNA            complement(26187..26263)
                     /locus_tag="ACZPB6_00755"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26227..26229),aa:Pro,seq:cgg)
     gene            complement(26370..26446)
                     /locus_tag="ACZPB6_00760"
     tRNA            complement(26370..26446)
                     /locus_tag="ACZPB6_00760"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26410..26412),aa:Pro,seq:cgg)
     gene            complement(26461..27450)
                     /locus_tag="ACZPB6_00765"
     CDS             complement(26461..27450)
                     /locus_tag="ACZPB6_00765"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR1183042.1"
                     /translation="MQNQQDPKALIKKVLAPIAEDMAAVDAVIRSELASDVPRMKEIS
                     EYITSAGGKRMRPALLMLICRALGYEGTLHQYLGATIELLHTATLMHDDVVDESDMRR
                     GRPTANSRWGNGAAVLVGDFLYTRSFQMMVKAGNLRVMEALSDAANRLSEGEVVQMVN
                     AHDPSVDETRYFRVIERKTACLFEAGARMAAAIADCGPEKEEAVAEYALALGNAFQIA
                     DDVLDYRGNAVDTGKNLGADLREGKVTLPIIYARESCTPEERAMIDEAIRLGDGDFPT
                     IAAIVLKSDALEKCRLRAEKELDRGRAALENLAPSIFRDSLLELLAFTVQRDR"
     gene            27769..28080
                     /gene="rplU"
                     /locus_tag="ACZPB6_00770"
     CDS             27769..28080
                     /gene="rplU"
                     /locus_tag="ACZPB6_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR1183043.1"
                     /translation="MYAIIKTGGKQYRVSVGDKLKVETLAVEAGAQVTLTEVLAVSGE
                     EGLKVGNPTIEGATVTATVLSHGRGEKVRIFKFRRRKHSMKSAGHRQNYTELKIEAIA
                     A"
     gene            28114..28377
                     /gene="rpmA"
                     /locus_tag="ACZPB6_00775"
     CDS             28114..28377
                     /gene="rpmA"
                     /locus_tag="ACZPB6_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR1183044.1"
                     /translation="MAHKKGGGSTRNGRDSKAKRLGVKVFGDAAVNAGGIIIRQRGTK
                     FHAGNNVGMGKDHTLYALVDGVVKFEVKGAFNRQYVKVEPIAA"
     gene            28665..29732
                     /gene="obgE"
                     /locus_tag="ACZPB6_00780"
     CDS             28665..29732
                     /gene="obgE"
                     /locus_tag="ACZPB6_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR1183045.1"
                     /translation="MKFVDEAKIEVQGGKGGNGAASFRREKFIPKGGPDGGDGGRGGS
                     VYAVADRNINTLVDYRYTRKFFAPNGENGRGADCYGAGGDDIVLRMPVGTLVRDTDTG
                     EVIADLNVHGARQLLAAGGKGGLGNLHFKSSINRAPKQATPGEPGQYRSLELELQVLA
                     DVGLLGMPNAGKSTFITAVSNARPKIADYPFTTLHPHLGVVRVGPEQSFVLADVPGLI
                     EGAAEGAGLGHLFLRHLSRTKILLHMIDAAPFNEETDPVQEAKALVLELEKYDPALAA
                     KPRWIVLNKIDLVEEADRDALIERYRSELAANGEPIFVISAATRQGCEELVKALAQRI
                     DDDRREEVAFADDTRFDPAEH"
     gene            29846..31159
                     /locus_tag="ACZPB6_00785"
     CDS             29846..31159
                     /locus_tag="ACZPB6_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIR1183046.1"
                     /translation="MQASVVERLFSLKENGTSVSQEVSAGVTTFMAMSYLIFVIPSML
                     ADGGIPRDAATSSVILVTVAVTLLMGLWARYPVGVAPGLGITAFFAYYVCGPAGFTWQ
                     AGLGAVFISGLVFFLLTVTRIRQFIVNAVPIDLKLSIVVGIGAFIALIGMKSAGLVIA
                     DPSTFVALGNVTQPEVQLSVFCFFLTAALMVKGFRAAMLIGILATTALGVILGVTELP
                     KGAWVSLTLPDISAVFMQMDLQGALSHGLLSIIFTLTMVDLFDNMGVLIGLARKCGFM
                     RPDGEIRNLDKAFITDSIGTMFSACMGTTTATSYLESASGVAAGGRTGLTAVTTAGCF
                     VLALFFIPLVGVVPAYATAPILIIVGALMMQEVVHIRFERLEIAIPAFLTIMTMPFTF
                     NIATGFGFGFVSYTVIHVLMGRWREVTPFMYLISACFVANFIMRT"
     gene            31493..32620
                     /gene="proB"
                     /locus_tag="ACZPB6_00790"
     CDS             31493..32620
                     /gene="proB"
                     /locus_tag="ACZPB6_00790"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR1183047.1"
                     /translation="MESLLKNAHRVVVKVGSALVTNDGRGLDIQAIERWATQIAELQK
                     MGREVILVSSGAIAEGVLRLGWEKRPSHVHQLQAAAAVGQMGLVEAYERHFRNHGIGT
                     AQVLLTHQNLTDREQYLNARMTIRELLRLGIVPVINENDTVVTSEIKVGDNDTLGALV
                     TNLVEADVLVILTDQQGLYTADPRKDPEAMFVSVATAGDPVLEKMAGGAASSLSKGGM
                     ITKILAAKRAARSGAGTIIASGRETDVLTRLARGELIGTHLRPSSTVLHARKQWIADH
                     LRASGSLVLDEGAAKALVAKHTSLLPVGVIGVKGEFVRGEVVSCLTEDGCEIARGLVN
                     YSSDDASRILRAHTEEIEERLGYMEQPEMIHRDNMVVLAGE"
     gene            32777..>33756
                     /gene="proS"
                     /locus_tag="ACZPB6_00795"
     CDS             32777..>33756
                     /gene="proS"
                     /locus_tag="ACZPB6_00795"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR1183048.1"
                     /translation="MRARSYFLSTLKEAPADADVISQQYMIRAGMIKKLAAGVYTYMP
                     VGLRTIRKIEKIIREEMDRAGAIELLMPLVQPAELWQESGRWEKYGAELLRFKDRHDR
                     DFVIQPTSEEVVTDIARQEIKSWRQLPVNFYHIQTKFRDERRPRFGVMRGREFTMKDA
                     YSFDRDAEGAAVSYDKMYQAYQRIFTRLGLMFRAVRADTGAIGGSRSHEFQVIANAGE
                     DVIAYCPDSDYAANIELAEARSLIDVRAAAAEEMVKRPTPGKEKCHDVAEFLGIPFEK
                     SVKSVVLAADRTDEKGNPLPAKIVLILLRADHDLNEVKAGHLPELKDGFR"
BASE COUNT         6073 a        10565 c        10802 g         6316 t
ORIGIN      
        1 cgggaaaagt cacggtgacg aggcatcccg acggctgtct ggtgttgtat ccggcgcccg
       61 tctgggagga aaagcgcagg gcgctggcga agctgccgta ttcggcgcgc gccttcgtgc
      121 gctatgttct cggcagcgcc gtcgatctgg agccggaccg tgcaggcagg attctgattc
      181 cctccgaact caggcagatg tgcggcctcg acagggaggt tgcgctgatc ggcatgggcg
      241 agcacttcga gttgtgggac agaagcacgc ttgaggcgcg tgaacgcgag gcgctcgcgg
      301 ccgagcttga tgcggcggac ttctcgttct gattgtgaag aattttcggg gcggagcggt
      361 tggttccgcc tccatcgttt gtgagttgcg ggcgtgagcg aggcattcat tcatctgtcg
      421 gttttgggcg aggaggcgcc ggaggcgctt gtgggagacc ctgacggact gtacgtcgac
      481 ggaacattcg ggcgcggagg tcattcgcgt cgcattcttg aaaagctgtc cccgaaggga
      541 cggctcattg cgttcgatcg cgatccccag gcggtcgagg ttgccagaag ctggacggat
      601 ccgaggttca cgatcattca cgagcccttc tcgacgatga cggagtctct tgccgcgatc
      661 ggcgttcatc aggccgacgg catctttctc gacatcggcg tctcgtcccc ccagatcgac
      721 gacgctgcgc gcggcttctc cttccgcttt gacggtccgc tcgacatgcg catggacacc
      781 acgcgcggca tgacggcggc tgagtggctg gcgcaggccg aggagcgcga catccgccac
      841 gtgatcagcc tttacggcga ggagcgcttt gcgggaccga ttgcgcgtgc gatcgtcgca
      901 cgcaggaaga cgaagcccct ggagacgacc cgcgagcttg cggatctggt tgcaggcgtc
      961 gtgcctcgca accgcaagga catgggccag catcccgcca cgcgcacctt tcaggccgtg
     1021 cgcatcgagg tgaaccatga gctcgacgag ctcaagcagg cgcttgacgc cgccggaggg
     1081 cttctgaagc ccgaggggcg tcttgccgtc atcaccttcc actcgctcga ggaccgcatc
     1141 gtcaagcact tcttcgacag ggcggcccac cccgagcgcg ggctcgatcc gaggcttcct
     1201 ctcacgaacg acatgctgcc caagccgctc ttcgacaaca ttgagcgcat caagccgtcc
     1261 gaggccgagt gcgaggcgaa tccgcgcgcg cgttcggcga ttctccgcgt cggcgtgcga
     1321 accgtgtcgc cctggcgtcc ggaggacggg gcatgcgcca ggtaagcaag atggacctgg
     1381 tcgtcgtggc cgtgctgggt ctcgtcctct ttgcgcttgc catcacgctc gtcaacgtcc
     1441 agtacagggc gcgcacgctc ttcgtggagc atgagcgcgc cgtcgacgtg ggccgcaggc
     1501 tcgccgacga cgaggcccac cttcttctca aggtccgcaa ggcgtcgctt cccggaagca
     1561 tcgctgacgg cgcgggtgcg ctcggactcg aggtggccag aggcgagaat acggtgaacc
     1621 tggtggtgga cgaacatcac agggtcacgt ttgcgcccga cacggccgca gctcaggctg
     1681 cggcggccga tggcaaggcg ggggcctccg agggctccag aggaggccgt ccatgaagct
     1741 gtcgcttctc gaatggatcg agcgttccgt gctcgccgtc tgggagaaga tcgcggactt
     1801 cctcgcaaag gaccgtgagg ccagaaaggc tgcgcccgag catgccgccg accccctcat
     1861 catcattccc attccgaaga accgcaccta ccttgcgttt ttcggaatcc tgatgctttt
     1921 cttcattctt gcgggcagaa ccttttatct gcaggtcttc aacaatgact tcctgcagaa
     1981 cgagggcgaa aagcgctttg cgcgcacgat cacgatccag gggccccgag gcgagatcct
     2041 cgaccgcaac ggcatcgtgc tcgcgtcgag ccagccctgc cgtagcgtct gggcggaccc
     2101 tgcgctcgtg ccccgcagca cgaaggagga gaagcagcag ctgcgcgagc ttgcgaagct
     2161 gctcggcatc aagtactcgg agctgctcaa gaagcttgac cacagctcgt cgcccaactt
     2221 cgtctatctg gcccgcaacc gcgatctcgg cctggccgag gccgtcggtg cactcaggat
     2281 ccccggaatc ggtatgtcgc ccgaaatgcg ccgccgctat cccgacggtc ccgccatggc
     2341 tcacatcgtg ggatacacgg gtgcggacga tcacggacag gaaggcgtcg aacttgcccg
     2401 cgaccatgtg ctggcagggc gcgcgggctc gaggcgcgtg atccgcgacc gtcacggccg
     2461 catcgtcgac gacgtctggg ccaaggaaag cgaggcgggc accgacgtga cccttgcgat
     2521 cgactccaga gtccagttca tcgcctacga ggcgctcagg gccgccatcg agcgacatca
     2581 ggctaaggcg ggcgccgtcg tggtggcgga cgtcaacacg ggcgagatca tcgcgctggc
     2641 caacgagccc acgtacgatc cgaacgatcg ttccacggtg cttttcgagc gtgtgcgcaa
     2701 ccgcgtgctc accgaccagt tcgagcccgg ctctacgatg aagccgtttg ccattgcgaa
     2761 ggcgctcgac atggggcttg tggatccgct cacgaccatc cagacgtcgc ccggcaagct
     2821 cacgatcggc gaccgcacga tcggcgatac gcacgactac ggccttctga cggtggccga
     2881 ggtcgtggcc aagtcctcca acatcggcac ggcgaagatc gccctcgaaa tctcgccaca
     2941 gacgctctat gagacgtatt cggagctcgg cttcggcaat gcgcccaaga tcggctttcc
     3001 cggcgcgacc tcgggcagac tgcgtccagg caagacctgg cgccccgtcg agcaggcgac
     3061 gatttcctac ggacacggcg tgaccgtctc cctcatgcag cttgtcaagg cgtacacggc
     3121 gcttgcgaga aacggcgacg tcatcgacct cacgctcttc aagcgaatgc ctggcgagac
     3181 tgccgcaggc acgcaggtct tcaagcctga aaccgcacgc cgcatgaggg ccatgatgat
     3241 ggcgaccgtc aagcgcggcg gcacggcctc caacgtcaat gtggcgggct actcggtcgc
     3301 gggcaagacg ggcacggcca acaaggtgaa gaacggacag tacacgaggg acgtcgtggc
     3361 gagcttcgtg ggcatcgtgc ccgcgaccca gccccgcttc atcgtcgccg tgatggtcga
     3421 cgagcccaag aaaggccgct tcggcggtct ggtggccgcc cccgtcttca acgaggtggc
     3481 cacgggtgcg ctgcgcaccc tgatggtttc gcccgacgac aagacggcgc ctgcgggcgg
     3541 cgggcttctc gcgaaggtta gacactgaca acgcaatgga ttgaacacaa gatgaacaag
     3601 tcgatgaacg aagtgacggc ctggctcaag gcccttgtgc atgacggcgc ccatctgcgg
     3661 ctcgacagcc gcagcgtgaa gccgggcgac gtcttcgtcg ccgttcccgg cctcaaggcc
     3721 gatggccgcg cattcatccg cgtggcggcc gcgcgaggcg cggcgggcgt gctgatggag
     3781 acgcgcaacg atgcgcccga cgccgagcgc ttcccgatcg ccagcctttc cgtcgacgac
     3841 ctctcgaagc acctaggaga ggtggcggcc gccttctacg gcgatccgtc ctcgaggatg
     3901 acgggcgtcg ccgtgacggg cacgaacggc aagacgacga ccacgcactg gatcgccgcg
     3961 cttctgacga acttcggcac gccctgcgcc gtcatgggca cggtgggctg caggttcgcg
     4021 ggcgagtcct tcgacatgcc cgcgctcacg acgcccgacg ccgtgagcct tcagggcgtc
     4081 tacgccgacc ttgaaaaggc tggcgcgaag gcctttgccg tcgaggcaag ttcgatcggg
     4141 cttgagcagg gccgcatgac gggatcccat ttcgacacgg ccgtcttcac aaatctgacg
     4201 cgcgatcatc ttgactacca cgttaccatg gacgcctatg ccgaagccaa ggccattctt
     4261 ttcgcctggc ccggcctcag gaccgcggtc gtcaatgtcg acgaccctta tgcgggactc
     4321 atggccgaga cggcgaggaa gaacggcgcg gccgtctggg cgacgaccct gacgggcgtc
     4381 gtgccttttg cggccgacca tgtcgtcgag gcctcggaca tcgaggccac cgaggcgggc
     4441 acgcgtttcc tgatgaaggt ggacggagct gcgacgtctg tgcaccttgc gcaggtgggc
     4501 ggcttcaacg tctcgaactt cattcaggcg gccaccgtgc tgcttgcaaa gggctgcgaa
     4561 gcagacaggc tcgcggcctg ggccgagtcg cttgcgtcgc ctccgggccg catgcagctc
     4621 atgcgcgccg agggcgcgcc tcttgcggtg gtcgactaca gccatacgcc cgatgcgctt
     4681 gaaaaggcgc ttgagagcct gaggcctgtg gcacaggccc gcggcggtcg cctctgggcg
     4741 gtctttggct gcggcggcga ccgtgacgca ggcaagcgtc ccgtcatggg cggcatcgcc
     4801 gtcaggctcg ccgacgaggt cgtcgtcacg agcgacaatc cgcgcacgga ggatcccgac
     4861 gcgatcatcg ccgacatttc ggcgggcgca cccggcgccc gagtcgtgac ggacagggcc
     4921 gaggcgattc atcacgccgt tgccgaggcc gacggagccg acgttgtgct catcgccggc
     4981 aagggccatg aggattatca ggaaatcaac ggcgttaagc accacttcag cgattccgaa
     5041 gtcgtgcgcg aggccttcaa cgaacgacgc gtgcagatgc tgcgcgcggt tgaggaagtc
     5101 tgatcatgga atgcctgcag cagcttcttg aagcgctcga gccggttgtc gttgcggcgg
     5161 ccggagactt ttcgcagtcc tacgagggct tttcgacgga cacccgcacg gtgaaggcgg
     5221 gaaatgtctt cgttgcgctt tcgggcgagc gttttgacgg gcacgacttt gttgtcggag
     5281 cggccgaggc gggcgccgtg tgcgccatcg tcgaacgcaa ggtggacgcc gccatcaccc
     5341 agatcgtcgt cacggactcg gggctggcct tcgggcttgc ggccaaggcc tggcgcggac
     5401 gctttgcgct tccgctcgct gtcgtcgccg gcagcaacgg caagaccacg acgacccaga
     5461 tgcttgcatc gatcctcctc gcgcgatggg gcgccgagcg catgtgcgcg accgaaggca
     5521 acttcaacaa cgaggtcggc gtgcccaaga tgcttctgaa gcttgccttc gagcaccgcg
     5581 gtgcggtgat cgagtgcggc atgaaccaca ggggcgaaat ggcgaggctc gccgactgga
     5641 cgcgtccgac cgtggcgctg cttaccaacg cgcagcgcga gcatcaggag ttcctcaggg
     5701 gcgtcgagga gacggcccgt gaaaacggac tcgtgatcgc cgccctcccc gagagcggca
     5761 cggcggtcta tcccgccgac gacgcatgcg cggacatctg ggccgacctc gcgctcgcgc
     5821 gcggcgtcga cgagctgacc tattcgactg atcccgacgt cgaggccgac gtcatgggcc
     5881 gagtcgagaa cggagagctc gtgatctcga cggacgaagg cgacgtggtg cgcacgaagc
     5941 ttcgcattac gggaagccac aacgtgcaca acgccacggg cgctgcggct gcggcttttg
     6001 ccatgggcat cggccccgag gccgttgcgg cggggctcgg cgccttcgag cctgttcccg
     6061 gacgcggcac gcgtttcgtc ggaaagcacc ttgttgtgat cgacgaggca tacaatgcca
     6121 atcccgacag cgtgcgcgcc gccatgaacg tccttgcggg cgaggcgggc ccgaggacct
     6181 tcatcctggg cgacatgggc gagatcggcg acagcgccga agaagcccac cgcgaggccg
     6241 gcgagtacgc cagatcgctc ggcatcgacg ccctctacgc ctgcggtccg ctttcccgtc
     6301 atgcggcgga ggccttcggc gaaggcggcc gctggttcga gaccagagac gccctcatcg
     6361 aggcactcaa gggtctcagg gggacggcga ccgtcaaggc gagccacttc atgggctttg
     6421 cggccgtcgt gcgcgccctg caggacaacg aagaataaac ccagcaatcc aagaacaaag
     6481 agagcctgag ccttcaggct ttgcaacatg cttctagaac ttttccgctc tctggccgac
     6541 ggtttcccgg ccctcaacgt cttcaactac ctgacgctgc gcgcggtgct tgccgcgctc
     6601 acctccatgg cgatcgggtt tgcgctcggt ccctggatga tcgcccgcct taagaacctg
     6661 catttcggac aggccgtgcg caccgacggg cccaagaccc atctcgtcaa ggacggcacg
     6721 ccgacgatgg gcggcgcgct cattctcttc gccatcacgg tttcgactct gctctgggcc
     6781 gatctctcca accgcttcat ctgggtcgtg ctcttcgtga cgctcggcta cggctccatc
     6841 ggctggatcg acgactaccg caaggtcgtg caccacaatc ccaagggcat gagcgcgagg
     6901 gagaagttct cgtggcagtc cgtgatcggc cttgccgcag ccttctacct tgtatttgcg
     6961 ctttccgttc ctgcgggcgg aacgctcacc gagacgattg cgggctgggt cgaggctggc
     7021 ttcccgctcg acatctcgag caaccttcac cttctcatcc cgtgcttcaa ggacatcgcc
     7081 cttccgctcg gcctggtggg cttcatggtc ttcacgtatg tggtgatcgt cggcagctcc
     7141 aacgccgtga acctcaccga cggcctcgac ggtcttgcca tccttccgag cgtcatggtg
     7201 ggcggtgcgc tgggcatctt cgcctacgtc gtgggacgtc ccgactatgc gggctatctg
     7261 atctttccct acattccggg ggcgggcgaa ctcgtcgtga tcgcaggggg cctgctcggc
     7321 gcaggtctcg cgttcctctg gtacaacgcc catcccgccc aggtcttcat gggcgacgtg
     7381 ggcgcgctgg cgctcggcgg ctgcctcggc accatggccg tcatcacccg tcaggaactc
     7441 gtgctcgccg tcatgggcgg catcttcgtc gtggagacgc tctccgtcat gattcagacg
     7501 gtctacttcc gcctctccca cggcaagcgc atcttcctga tggctcccgt ccatcatcac
     7561 ttcgagctca agggctggaa ggagacccag gtcgtcacgc gcttctggat catcacgatg
     7621 atcctcgtgc tcatcggcct ttcgacgctc aagatccgct gacgggtagg gcgttccgcg
     7681 gggtttgaaa aggacagttt gatcatgaag caggaagttg acaagaagac cgccctcatc
     7741 gttgggctcg gcgcctctgg gctcgcctgt gcgcatcatc tcggtcgccg cggatggaaa
     7801 atccgcgcgg ccgacacgcg cgaggcgccc gccggtgcgc aaaggctcgg ggccgagatg
     7861 cccgatgcgg aaatccgcac gggcggcctg cccgaatcgc tcctcgacgg cgtttcgctc
     7921 gtcgtgatgt cccccgggct ttccacccgc ttcggtgcgg ctgcaccgct tgtcgcgagc
     7981 gccagggccc gcgacattga ggtcgtgggc gagatcgaac tcttcgccag agcgctcgca
     8041 gaccttgagg cccgcacggg ttaccgcccc gtcgtaatcg gcgtgaccgg cacgaacggc
     8101 aagacgacca cgacgacgct ggtcacgaag atggccgccg agggcggccg tcgtgcgacg
     8161 gctgcgggca acatcggccc caacgccgtg accgagctcg acaaggcgct cgatgcaggc
     8221 gttctgcccg aggtctgggt gcttgagctc tcgagctttc agctcgacac gacgtcgagc
     8281 ctcaagtgcg tatccgcagc tgttctgaac gtcaccgagg accaccttga ttggcacggc
     8341 gacatgaagg cctacgccga ggcgaagggg cgcatcttca agcaggacac cgttcgcgtc
     8401 gtcaatcgcg aggacgccga gacgctccgt cttgcacagg gaggtcccgt gcgcacgttc
     8461 ggcggcggtg agcccgtcaa ggcgggcgaa tacggcctcg ccagaggggc tgacggcctg
     8521 ctgtggcttt ccaggcttga tgagggcgcg tctgagccca cgctcttctg ccctgaaaag
     8581 tcgcttctca ttgccggccg ccacaacgcg atgaacgcac ttgccgcgct cgccctcatc
     8641 gaggctgcag gcctgcccgt cggacctgcg ctcgccgcgc ttgccgcgta cgggggcgag
     8701 ccgcatcgag tcgagcgcgt gctcgattgt gacggcgtta ccttcatcga cgactcgaag
     8761 ggcaccaacg tgggcgctgt ggccgctgcc gttaagggct tcgcagctca gggccgccgc
     8821 atcctcatca ttctcggcgg agacggcaag gggcaggatt tctcgcctct ggtcgaagcc
     8881 ctgaaaggca cggctggcgc cgtcgccctc atcgggcgcg acgcgcccct gatcgaggag
     8941 gcgctttccg ctacgggcat tcccatggcg cgtcagacga cgcttgagga agccgtgtac
     9001 tggctctggg caaggcatgc gcagggagac gtgcttcttc tctcgcccgc ctgcgcctcc
     9061 tgggacatgt tcagggacta tgccgagcga agcgcccgat tcattgcctg tgcccgcgcg
     9121 attgcggccg aggagcaggc gtgctgagct tcggacggaa aaagaagaag gaggactggc
     9181 agacgcaggg gcgcgccgtc gcgacgctca aggagcgtgc gaacgcgagc cttgaggtga
     9241 tcatctgcgt ggccgcactg ctcggcatcg gcaccgtcat ggtgtactcc gccacgtccg
     9301 tctttgccga caactcccgt tacggcgtcg acgcgctcta cttcgtcatg aagcatctgc
     9361 agagcatcgt cgtcggtgtc tgcgcgggct ggatcgtcac gaagattccg atgagcttct
     9421 ggcagcgcta cgcgctctgg gtgttcttcg gggcgctcat cctgctggtg tgcgtgctgc
     9481 tgccgggcgt cggcaaaacc gtcaacggcg cgaagcgctg gatcagtctg ggcgtgatga
     9541 acctacaggt ctcggaaatc atgaaggtcg cggctctgat catggctgca tggttcgccg
     9601 tgatgaggca ggaatacatg cattccttcc agaagggctt catcccgatg gcgttcgcca
     9661 tggtggccat tgcggccctc atcattcagc agcccgacct cggggccatg gtggtcatcg
     9721 tcgccgaaat catgggcgtg ctgtttttag gcggcctctc ctttaaaatc ttcacttttg
     9781 tgatctcggt cgtcgtgggc ttcgtcttct ggatgatcat cgacacgccg tggcgcttgg
     9841 gcagaatctt tgcatactgg gatccttggg ctcccgagca cgtgctcgac aaggcttacc
     9901 agctgtcgca ctccctgatc gcattcggac gcggagaact cttcggcgtg ggcctcggcg
     9961 gctcggtcga gaagctctac taccttcccg aggcgcacac cgacttcatc atggcggtga
    10021 tcgccgagga gacgggcttc gtgggcgtct gcctcgttct cttcatttac tattggctta
    10081 ttaggcatgc gttcgagatc ggacgcgtgt cgatcaaatt ggaaagattc tttcagggtc
    10141 ttcttgcgca gggcatcggc gtatggttcg gcgtgcaggt cttcattaac gtgggcgtgg
    10201 cctcggggct actgccgacc aagggtctga cgcttccctt cgtgagcttc gggggcagcg
    10261 ccttgctctc gggcctcatc gccatcggcc tgctgctgcg cgttgacaag gaaaacagaa
    10321 tgctgatgcg cggaggtcag gtgtgagcac ggattcgaaa catttggtga ttcttgcggc
    10381 cggtacgggc ggtcacgtga tgcccggcct tgccgtggcg aaggtcatgg ccagcagggg
    10441 atggaagatc tcctgggtcg gcacggtcgc aggcatggag cgcgggctcg tggagcgcca
    10501 cggcattccg ttccacgcac tcgactttca gggcgtgcgc ggccgcggtc cgctcggcgc
    10561 gatcaagggc ggcttcaagc tgatcggcgc attcttcgcg tcgatgaagc ttctcaatga
    10621 gctaaagccc gacgccgtct tctcgacggg cggctacgtc gccgtgccgt tcggctttgc
    10681 cgctcaccgc aggaagcttc cgctcgtgat gatgaactgc gatgcggacc tgctgatgtc
    10741 caccaatacg ctgcttccct tctgcgacgc gctcgcctgc ggctttgccg gcggcgcgag
    10801 gacgtttgcg aaggaaaagg gccgcacgac gggcaacccc gttcgtgagg agattgtcgc
    10861 cgtctcaccc ccgcaggacc gcatggcgaa ccgcacgggg ccgctgcgcg tcttcatctt
    10921 cggcggcagc ctcggcgccc aggtcttcaa cgacaacatg cccgccgtct ttgcggccat
    10981 tccgcaggag cagcgtccga tcatcgttca tcagacgggc aaggaccgcg acgcgcccgt
    11041 gcgcgaggcc tatgaaaagc tcggcgtcaa ggccgaggtc gttcccttca tcaacgacat
    11101 ggcggcccga tacgaggact gcgacgtggt gatctgccgc gcgggcgcga cgagcgtctc
    11161 cgagctttgc gcggcgggcg tggcgagcat tctcgttccg ttcgtcgcca agacgacggc
    11221 gcatcagacg ggcaacgcga agtacatggt cgagcgtcat gccgcgatca tggtgccgca
    11281 gcccgagttt acggtcgagc gcatggccga actcatctcg gggctcaccc gcgaccatct
    11341 gcttgagctt gcgaagaacg cccgtgcgac ggcgcgtccg caggccgccg aggccgtggc
    11401 gaacctcatc gtcgaaaccg tcgcccgcag gacgggagcg caggacgcca agtaaaggat
    11461 tgacgggcgg gatccctttc ccgccctctg aggagattca agcgtgaagt ttttggtcaa
    11521 ccatctgcat ttcgtcggca tcggcggtgc gggcatgagc ggcatcgcag aggtgatgct
    11581 caatctcggc tacgtcgtga gcggctcgga cctctcgaag agcgccgtca ccgagcgcct
    11641 gcagtcgctc ggtgcgcgcg tcttctgtgg ccacgacaaa aagcacatcc gcggcgcgga
    11701 cgccgtggtg atttcgtcgg cggtgcatga ggacaacccg gaggtcgtcg ccgcccgcga
    11761 gctcggcatt cccgtcgtgc cgcgcgccgt gatgctcgcc gaactgatgc gtcttcgccg
    11821 cggaatcgca attgcgggcg cccacggaaa gactacgacc acgtcgctca ctgcgtcgct
    11881 gctcgccgca ggcgatcttg atccgacctt cgtgatcggc ggccgtctca acagcgcggg
    11941 tgcgaacgcc aggctcggca cgggcgaatt cctcgtcgcc gaggcggatg agtcggacgc
    12001 gtcattcctc aacctcacac ccgtgatttc cgccgtcacc aacatcgacg aagatcacat
    12061 ggacacgtac ggccacagct tcgagaacct gaagtccgcc ttcgtcgact tcctcgagag
    12121 gctcccgttt tacggcgtgg ccgtgctctg cgccgatgac gagcatgtgc tcgagattcg
    12181 tccgcgcgtg accaagcccg tgattctcta cggtctgtcg gccgacgctc aggtgcgcgc
    12241 gatcgacgtg cgcgccgtcg gcacgcagat ggccttcacg atcctgaggc ccgatcatcc
    12301 gccgcttccc gtcgtactca acctgccagg catccacaac gtgaggaatg ctctggccgc
    12361 cgtggcgatc gccaccgtgg tgggcgtctc cgacgaggcc attgttcgtg gcctctcgga
    12421 attccgcggc gtgggccgcc gcttcacgca atacggcgac attcccgtgc gtgccgccga
    12481 cggaagtgtg acgggccact acaagctcat cgacgactac ggtcatcatc cccatgaaat
    12541 ggcggcgacg cttgccgccg tgcgcggcgt ctggcccgaa aagcgcgtca tcgtcgcttt
    12601 ccagccgcat cgctacacgc gcacccgcga ctgcttcgag cagttcgtcg acgtgctcga
    12661 ggacatcgac gggctcgtgc tcgccgaggt ctaccctgcg ggcgaagctc tgatcgaagg
    12721 ggcggacagc gagcatcttg ccgcagccat tgccgccaag ggcttccacc gacccgtcgt
    12781 cgtgcccgtc gagggcgtgg ccgatgcgat cgcgggcatg gtgcgcgacg gcgacgtcgt
    12841 catcaccatg ggcgcaggct ccatcggtag ggtcgcgcct gcgatcgccg acggcgcgaa
    12901 gtgacgcggg aactttgaat tgaatcgaat gaattgaacg gaaaggatta gtgaagatgg
    12961 aaaacttagg tcgtgtcgtg ctgctgatgg gcggcgtttc ctccgagcgc gaggtctctc
    13021 tgatgtccgg cgaaggcgtc cacaaggcgc tcgtgtcgaa gggcgttgac gtcctcaagt
    13081 tcgacccgaa gacggacagc cttgagacgc ttgaaaaggg tggcttcgac cgcgcgttca
    13141 tcgcgctgca cggccgtctc ggcgaggacg gcaccattca gggcgtgctc aactatctcg
    13201 gcatcccgta tacggggccg ggcgttacgg cgagcgccat ggccatcgac aaggatctga
    13261 cgaagcgcgt gtggcgcagt gcgggcattc ccgtgcccga gggcgtcatg attgatccga
    13321 aggcggacga cgccgtgctt gaagaggcca ttgcgtgtct cggcgcgacg ggtctcgtgg
    13381 tcaagcccgc tcacgacggt tcctcgatcg gcgtcaccaa gctcgccgag ccgaccgtcg
    13441 ctgccctcag aaaggcgctt gaggacgctg cgggcgtccg cggcgacgag gtgcttgtcg
    13501 aggagtacat ccacggcagg gagttcacgg tcgccgtgct tgacggtcgt gcgcttcccg
    13561 tgattgagat ccgcgctcct gaaggcgact acgacttcaa caacaagtac ttcggcgacg
    13621 cagtgcacta cgactgcccc gcgaagctcg aagacgaagc ggccgcgacg ctcggctcgc
    13681 tctgcgaacg tgcgtttgcc gccgtaggcg cacgcggctg gtcccgcatc gacgcgctgc
    13741 agcgcgccga cggctccttc gcgctgctcg agatcaatac gtcgcccggc atgacgcctc
    13801 attcgctcgt gccgatggcg gcccgtgcgg tcggcatgga ttatgccgac ctgtgcctca
    13861 aggttctgtc gctcgcccgc tgcgactgag cgcgggagcg gatgacagac ggagtttcgc
    13921 aggatgaagg ctgagagtcc cgaaaacccc gcggaggccg tcacgcctga agaggcgatc
    13981 gagcggcgtg ctctggcgcg tgcgcaggag gaggcccgcg aggcccgccg cgcaagcctg
    14041 cgcttctgga cgaagacggt ctgcggtctg gctgcggcgg ccgtcgttgt gcttggcgca
    14101 ggccgtgcgg ccgatgcggt gatgcatctg cccgtcttcg acttctcgac tctggaggtc
    14161 gagggggatg ttgcgcgcgt gcccgtcgag cgcgtgaagc aggcggtgga gcccgtcatt
    14221 cggggcaact tcttcaccga ggatctcgac gaggtccgca gggcggccga gacggtgcct
    14281 tgggtgaagc gtgccgtggt gaggagagtc tggcccgacg gccttcgcat tgccgtcgaa
    14341 acctacagcg ccttcgcggt ctatgaggac ggtcgtcttg tcgatccaga cggcgtgctc
    14401 ttttccgcca atcccgacga gcgcgacaac ccgtccgatc cgctgcccaa cttctacggt
    14461 cctgcaggac agaccgctca gatcgcccga tactacaggg agttctcccg cgcgttgagc
    14521 tccatcggcg cgacggtgac ggacgtcgcc tgcagcgatc gcggcggctg gagcctcgtg
    14581 atggcttcgg gcgacattcc cgcaacgcgc gtggagctcg gcagggagga cgcaggcacg
    14641 aacatcgtgg cgaaggttgc cgatctggcg gcggcctacc cgagggtcgt cgagctgatg
    14701 gacggaccgc ccgcctccat cgacctgcgc tatgacaggg cgtttgcggc cacgcttccc
    14761 gaccgcgaag ccattcgaaa actgcgggaa gccgaacaga gaacggatac ggatgtcccg
    14821 gacgaagagc ctgggagcgc acagcacgat gaacaacccg aatgattatc ttgtcggtct
    14881 cgacatcggc tcccacaagg tgctgtgcgt cgttgcgctc ccgtcgccca agggcgaggg
    14941 gctctaccgc atctgcggct acagcttcag ggattcgcac ggcgttcgaa acggcatcgt
    15001 gacggatctc aatgccgccg tcgacgacat caaggcggcg gtgcgcgagg cgcgctccgc
    15061 aggcaatctt cccgagcttt cgaatgcttg ggtcgccatc ggcggctcct cgctcacgag
    15121 cgagaactgc ctgggcacgg ccgtcgtgcg aggcaatgag gtgaagcccg cggacgtcga
    15181 ggcggcggaa aacaatgcgc gcgagcacag cctgcgtcag ggcaagcggc tcatcaagat
    15241 gatcccgcag ggctattcgt gcggcgatac gttttcgcag acgccggtcg gtctggtggg
    15301 cgacaaggtg acggcctact atcatgccgt ttacggctcc gtgaagaacg ccgagaacat
    15361 gaagcgcagc ttgctgcgtt ccggcatcga acttgcggga tatgagcctc atccgatcgc
    15421 cgccgcgctt gcggtgagta cggaaagcga ccgctacaac ggcacgctcg tcctcgacat
    15481 gggggcagag acgacgtcga tgacgctggt gtacgagcac aggacgcttc tgacggccgt
    15541 ccgtcccttc gggtcggaat tcttcacgcg cgacctgagc acgatcttcg gcgtgtcgct
    15601 cgaccaagcc gaagagctca agatccgctt cggaagctgc agcatggacg gcgtgctgcc
    15661 gggcgaaagc gtccgcccct cgctggagga tgtgcgcggc acgccgctct gctcgcgaag
    15721 cctgatcgtg cagaccctgt acgagcgcgc gaaggagttc ttccgcatct atcgcgacgt
    15781 catcgagaag gcgggccatc tcgacaaggt ccatgccgtg atcatcacgg gcggaggcgc
    15841 gaaccttcgc gccatcgacg aagttgcgcg agacgtgttt ggagttcccg tgcgcattgg
    15901 tgcgccgctc tgctttgatg agaagaatgc tctctccatg aggccgaacg ccagcgttgc
    15961 cgcaggcctg ttgatggcgg ccgacaagac gagggccgca ggcgacagcc gaagctacag
    16021 aacgccgtct ctcctgaacc tttcgggaag gataagaacc gtgtttttcg gggactactg
    16081 agcccgcctg ccgaagctgt tcgaggcggc tccggcaagc agaaaagcca aaagtccgcc
    16141 ataattaaaa aattcgacat tttggggtag aaaatgcctt ttgagatcct gcaggaactt
    16201 gatcctctga acaccgtcat caaggtgatc ggcgtgggcg gcggcggtgg caatgccgtc
    16261 gagcacatga tcacgcatgg tgcgaagggc atcgaattta tcgccgcgaa cacggaccat
    16321 caggccctgt ctcgttcgcg tgcgcacgtg aacatccagc tcggctccac gggcctcggc
    16381 gctggtgcca ggcctgaagt cggcgcggcc gcagctcagg aaaagcgcga gcaggtggcc
    16441 gaggccatcc gcggcgcgca tctgctcttc atcaccgctg gcatgggcgg cggcacggga
    16501 acgggcgctg ctcccgtcat cgccgagatc gccaaggaaa tgggcatcct cacggttgcc
    16561 gtcgtcacga agcccttcag cttcgaaggc ggccgtcgca tgcgttgcgc ggaaaagggc
    16621 atcgagaacc tcaaggacaa ggtcgactcg atgatcgtga ttctcaacga gaagctcgag
    16681 agcgaatgcc ccccgaacgc cacgatgaag gaatgctttg aaatgagcaa cgaagtgctc
    16741 tacaaggcat gcgtgggcat tgcggaaatc atccacacgc ctggcaccat caacgtcgac
    16801 ttcgaggacc tgaagacggt catgagcgaa cgcggcacgg ccatcatcgg tctggcgtcc
    16861 gcctccggtc cggaccgcgc ccgcgtggct gcggaaaagg ccatcgcctg tccgcttctc
    16921 gaaggtgcga acctccaggg cgcccgcggc ctgctcgcct acttcacggg caacgagtcg
    16981 gtcacgcttg ccgaaatcaa ggaggccatg gcggtgctca acaccttcgt ccacaagagc
    17041 gccatcgtga tcttcggctc cgccatctcc gaggagatgg gcgacgagat ccgcgtgacc
    17101 ctcgtggcga ccggcctcga ccgtccgctc gatgcgaacg aacccggctc gggcgttcag
    17161 gctcccgcag tcttcaacgc ctcgtccgaa gcccccgctg gccttcagac cccggtcttc
    17221 ggccagacgc tggaggcccc tgcgcccgaa cccgccgctc agcaggggga tgctgcggtt
    17281 gacgtgtcca agttcgtcat tccgacctat ctgaagtaag ccgctccgtc gggcacggcc
    17341 cgttcgggga cccagaagga agccggcgcg tcaagcgccg gttttcgtac ggagatcact
    17401 catgttcaag cagcgcacgc tcaggcagtc cgtcgagacc gtcgggatcg ggcttcacag
    17461 cggccgcaag gtgcacctcg tgcttcgccc cgcggaaccc gacacgggca ttctctacag
    17521 gcgcgtggac attcgtcccg ccgtcgagat tcccgctcat cccctcaacg tgaacgacac
    17581 gcgcatggcc acgacggtca acgtcggcaa ggccgccgtg gcgaccatag agcatctgat
    17641 gagcgccttc aacggtctcg gcatcgacaa tgtgatcatc gaggttgact ccgccgagat
    17701 tccgatcatg gatggctcgg gggcgagctt cgtcttcctg cttcaccagg cgggcatcgt
    17761 cgagcaggac gcgcccaagc gcttcgtgcg ggtgctcaag cccgtcgagg ttcgcgaggg
    17821 cgacaagctc gcaaggcttg agccgcacga gggctttcgt ctcacgttca ccatcgcctt
    17881 cggtcatcct gcgatcgacc gcacggagca gaaggcggac gtcgacttct cgcgcgtcac
    17941 gtacgagcag gcggtctctc gcgcccgcac gttcggtttc gtgcaggacg tggagatgct
    18001 tcgccgcatg gggctcgctc agggcggttc gctcgagaac gccatcgtga tggacgagtt
    18061 ccgcattctc aatccgggcg gcctgcgcga gaacgacgag ttcgtcaagc acaagattct
    18121 ggacgcaatg ggcgacctct acgtgcttgg tcatccgctt ctggcgcatt acgttgcgca
    18181 caagtcgggt cacggcctca acaaccagct cctgcgcgct cttcttgcag acgagacggc
    18241 ctgggagtgg gcgacgttcg acaaggccga ggactgtccc gtcgacttct ggacgcagaa
    18301 gaccgatcag gtcagaccgt aaagtccctc tgcaatcgca cgcgcgtcct gcggacgctc
    18361 cgacagcgcc ttggcggctt ccagaagctg cgcaaggcgc ttcgtgtttt caggcgtcgg
    18421 attctccagc atggaaaggc gttcggcctc tctgcggatg tcggcgtgaa gggcctcggg
    18481 gtagtgggcc tcatcgggag cgagggttgg aatgtggctc atcagggcct tgcgcatgcg
    18541 cggggacggc gtttcgcgcg cgagcgacgc gatggcggcc gctgcagcgg cggaaacgcg
    18601 cggtgcggcg ggcggcgcct gaggggcttc ctcgtcttcg ggtgcgacga gagcggcctc
    18661 ggcggcctgc gcgtccgagg gcagaagctc aagcgcgcgg ttgatggcgt cgagtctggc
    18721 gtcgattcgc tcgagcctgt gcacggcgac aagcctgcgg tccttctgtt cggccagaag
    18781 ctcaagcttt cgctcgcgct cggcctcaag tctcggatag tctctggcct cctcctcggt
    18841 ggggatgatc gcggcgttga tgccgtgcgt caggtccgct acgagcgtcg tgaggcgcat
    18901 gcgctcggtg ctcaggtcga tcgattccct gccgagacgc tcctcgagaa gacgccggcg
    18961 gttttcctcg ccgggcttga tcacgttgcg aagtctttcc atcgtggcct tgagggcggg
    19021 atcctcgatg tcgtcaagaa tgcccgagat ggcggcggca cccactgcgg acggcgcgcg
    19081 ctcgatccgc ggaataggaa cgcccgcctg gggtgcgggg cgcttcggaa tgatgcgggc
    19141 gtcgacggcg gtgacgggaa gacccgccgc tgctgcggcg gcggccagtc tgggggtgag
    19201 ctgtctgatt ctgttttgtt ggacggcgct ttcagccaga agcacgagcg tgtgcccctc
    19261 gacgcggcag taggcgggat ctgtgaagtc gatgctcatc ctgaaggggc gaagcacgtg
    19321 ggctgcgact gcgcctgcgc gctttgaaag cgcgtaggag gccatcagcg gctgcagctc
    19381 gtcgcgggcg aggcagctgt cgatcgtctg tcgtccgatt cttcggcgga tgaagtcttg
    19441 tgtcacattg cgaccttgtt gtaagagaag gaaaacgcct acagaatagc ccgtaggtac
    19501 aaatcaggtg gcggaattct gtgcgataat ccaacagcta tgcaggtcgg ggcctgcgga
    19561 ggggcgctac gcacgccttt ccaacaggga ggaggctccg gcgcaaccga aaacagggta
    19621 gcacggaaca gcgccggcgt gagcggcgcg gccatgccgc atatggttcc gtccgtgccc
    19681 tcagatccat tctggttaca cacaatgttt gacgaacttc tgacaaaaat cttcggtagt
    19741 cgcaacgacc gcctgatcaa gcaatatcgc cgcaagtgcg atgccatcaa caagctcgag
    19801 cccgccatgc aggcgctctc ggacgaagag ttgaaggcca agacgcagga attccgcgac
    19861 cgcatcgcca agggcgcgac gaccgacgag ctcctccccg aagcttttgc cgtggttcgt
    19921 gaagcctccg tgcgcgtgct cggcatgcgc cacttcgacg tgcagctcat cggcgcaatg
    19981 gttctcaacg acggcaagat cgctgaaatg cgcacgggcg aaggcaagac actcaccgcc
    20041 acgctcgccg tgtacctcaa cgcccttccc ggcaagggct gccacgtcgt gaccgtcaac
    20101 gattaccttg cctcccgcga cgcggactgg atgggccgcc tctacaactg gctcggtctt
    20161 tcggtcggca agatcctctc gaatcagaac aacgaggaaa agcgcgccgc ctatgcctcc
    20221 gacatcacgt acggcacgaa caacgaattc ggcttcgact acctgcgtga caacatggag
    20281 tacgacgtcg ccgctcgccg ccagcgtccg ctctactatg cgatcgtcga cgaagtggac
    20341 tcgattctca ttgacgaggc ccgcacgccg ctcatcatct ccggccccgc cgacgacaac
    20401 acggaactct atcgtgccgt caacgacatt ccgccgctcc ttacccgcca ggctgaggaa
    20461 aagggcgagg gcgactattg ggtcgatgag aaggcccatc aggtctacct ctccgaggcc
    20521 ggtcacgagc atctcgagca gattctctcc gagcgcggtc tcgtgcagca gggcgagtcg
    20581 ctctatgcgc cgaagaacat catcctgatg caccacctca acgccgccct gcgtgcgcac
    20641 acgctcttcc accgcgacca gcactacgtc gtgcagaacg gcgagatcgt catcgtcgac
    20701 gagttcacgg gccgcctgat gccaggccgc cgctggagcg aaggtcttca ccaggccgtc
    20761 gaggccaagg aaggcgtgaa gatccagcag gaaaaccaga cgatggcgtc gatcacgttc
    20821 cagaacttct tccgcatgta cgggaagctc tccggcatga ccggcacggc cgacaccgaa
    20881 gcctacgaat tccaagacat ctacggcctt gaaacggtcg tcgtgccgac gcatcgcaag
    20941 atggtccgca tcgacgagca ggacaaggtc taccgcacgg tcaacgaaaa gtaccgcgcg
    21001 atcatcgacg acgtgaaggc ctgctacgaa cgccgtcagc cggtacttct cggcacgacc
    21061 tcgatcgaaa actccgagct tctctcctcc atgctcacga aggaaggcat tccgcacaac
    21121 gtgctcaatg ccaagcagca tgacagggaa gcccagatcg ttctcgaagc aggccgtccc
    21181 ggcgtcgtga cgatcgccac gaacatggcc ggccgaggca ccgacatcgt gctcggcggc
    21241 ggcatcaaca aggctgtcga cgccattcgt gccgacgaga cgcttactga agagcagcgc
    21301 gaggcgaaga tcgccgaagt gaaggccgaa tggcagaagc ttcacgacat cgtcgtggag
    21361 gctggcggcc tgcgcatcat cggttccgaa cgtcacgagt cccgccgcat cgacaaccag
    21421 ctccgcggcc gtgcaggccg tcagggcgac ccgggtagct cctgcttcta tctctcgatg
    21481 gaagaccagc tcctgcgcat cttcggcggc gaccgcatgc gtgccatcgc cgataagctc
    21541 cgccttgaag aaggcgaggc catcgagtcc aagatgctca cccgcatgat cgagtccgcc
    21601 cagcgcaagg tcgaaggccg caactacgac atccgcaagc agctgcttga gttcgacgac
    21661 gtccagaacg accagcgtca cgaaatctac ggcctgcgca atcagatcct tgagagcgag
    21721 gacatctccg agctcgtcaa gaacctgcgc gaaggcctct ttacggacct cttccgcgcc
    21781 tatgtgccgg ccgagaccgt tgaggagcag tgggatctcg acggtcttca gacgaagctt
    21841 gccggcgagt tcggcattgt gatcgacttc aagcagcttc ttgactcgaa cgaccagttc
    21901 acggacgacg acctgctcga ggcgcttctc aagcaggccg aggcccagta cgtcgagaag
    21961 gagcagatgg tcggtcacga ggccttcacg ggttttgccc gctccattct ccttcagatt
    22021 ctcgaccagc tgtggcgcca gcacatttcc gcgcttgagg cccttcgtca gggcatctac
    22081 ttgcgcggct atgctcagaa gcagcccaag caggaataca agcgcgaggc cttcaacatg
    22141 ttcgagcagc tgctcgacca gatccgcgag accctcgtgc gcgtgctcat gcaggttcag
    22201 atcaacgtga agcccgccga agagcccgcc cagcagcagc ctgtcgagga acctgctccc
    22261 gccgaggcga caggcagcgc gctcgagaac atgcccaagg aggagctcga tcccgcgctc
    22321 ttcgcgcatg tcggccgcaa tgatccgtgc ccgtgcggct ccggccgccg attcaaggac
    22381 tgtcacggcc gtctctcctg agaggctgac gaatagtcct gaatgacttt cagagcccgt
    22441 cttcgcgttt cgtgaaggcg ggcttttcct tggcggtcgc caatgtcaat gctggagatt
    22501 cggacatgag gaatgaacga tcaatttcgc tccccggcct tctactgggc ttttccgttg
    22561 atgggggcgt tgcggcggcc gctgttcctg cggctgcgcg cacggttctg tcgcttccgc
    22621 aggttgcgct tggggacggc acggcgcagg ctctgaggat gcgcaggatg tcggggtcgc
    22681 ggacgggctg cattctgatg agcaggggat atccgatgtt cggcgcgggc ggtctctctg
    22741 atgcggggtt gagcgcgttc aggcatgagg cgctcagacg tctggagctt cccgaggatg
    22801 cgctcgccct cctgcttcag ggaacccgca gggagctgac gccggtcgga gggcttgtcg
    22861 agcgatgcac cgaagcctgg aaaagagcag ggggcgaggc actgctcccg gaagggtgga
    22921 cgggattcga aatgcccttc atgacgcagg acggcgtgat gagggtcgga gcactttcgg
    22981 caggcggtgc ggtcccgacg gaaaaccgcg tcctgctgct tgtgaccgat gtccctgtgc
    23041 gggaggtgat cctgcagcgg atcgccgaca gagcggccgg tcctctcttt gcgtggctca
    23101 gggctgttgg cgtgctgtct gccgctgatg tcgtcatggt cgtgtcggcg gcgggtcgaa
    23161 ggcctgtggc gtcaatcgat gatcctgctg cggaaatgct tgccagagct cttgaactcg
    23221 ccgtcggacg cgtgtcggag gcatgggcgg gggcgctggg gaggaacatc cgcgtgcggc
    23281 tcgagggact gcgggacgaa gaggaggcgg agcatctgat cgcggccctt gcgggcgtcg
    23341 gcggatggct gaggggagcg ggaacacctc gggaggtctc cctagtgctt cgggccgcgc
    23401 tcgagcagag cgctcttccg ggacttgagg cttcgggatt tgccgtgcgg ctgggcgagt
    23461 gcgccgacgc cctgtcgctt gcgtcggctc aggagctttc gaatctgcag gaaggcaggg
    23521 cggccatcac gcttttcatg gctcggggca atctttctac gacattttgc gtgtagcgaa
    23581 acgtctggag aaggagttaa gattttctct gtgcgcctcg tttggggggc ggtcagaaag
    23641 ataaaggaaa agcatcgtga gtgaagacaa ggttgtggat gttgccgtcg gcgtgctgat
    23701 tcgcgaagac ggccgagtgc ttctcgcctc ccgccccgaa ggcaagccct gggctggata
    23761 ctgggagttc cccggcggca agcttgagga tggcgaaacg gtgcatcagg cgcttgtgcg
    23821 cgagctcgac gaggagcttg gcgtgcgtct ggccgattcc ttcccctggt ttgtgaagga
    23881 gcatcgctac gagcatgccc acgtgcgcct tcacttccgc cgttcgcgcg acttcttcgg
    23941 cgaggcgctt tccaaggaag gacaggactt cggcttttat gcggccaacg agcgcacacc
    24001 gggcgttctt cttccgatcg accagaacat tctcaaccga gtcgatcttc ccgatgtctg
    24061 ggaggacagc acggccgtgc tgacgctttc cgaaaacgcg cttcacgcca ctgtggttcg
    24121 cgaccgcaag tatcgcttcg tcggagcccg ctgcggcaat cttgacgacg tgatgaaggc
    24181 cgtcgccatg gacttcgact tcgtgatcgt caagcccgag gtctttgagg ccacgctctg
    24241 gaagggcgag cctcgtcttc cgacctatgt tgaagacgtg cccgcctccg acctgcgtct
    24301 ctggcaggac cgtggtgcgc acggcgtcaa gccctgacga ttcgccttcc gaggcttcga
    24361 aaatgaaaaa aaagcctgtt cagtgctgaa caggcttttt tgtcggcggg cgttcctgca
    24421 ggaagaggcg ttctcagcga cgtctgccgc ccttgcgctc ctcgcgttcg gcggtgtagc
    24481 gggcgacgct ctcaagctct tcgggcgtca tgcggtcagc ggagccggga tcgccttcga
    24541 tcacatagcc gtctgaggcc cattcgccga ggtcaatgag cttgcagcgt tccgagcaga
    24601 agggacgcca gggattggcg gaggagtatt cgatctcctt gccgcaggtg gggcatttga
    24661 ctttcatgat gtttttcggt caggaaggcg tgcagagccc gagacggaac ggaatctcct
    24721 ccgtgcgggc cagatgcagc ttgtggtttt cgtcagggcg cgagaagcgg atccagagca
    24781 tgtacttgtt ggcgctcacc tcgggaacaa ccgtgctgtc ggcgggaagc cagatctgga
    24841 cgagggcgca gttctgattg cggttcatcg agaactggta cgtgccgttg acggctgtaa
    24901 ggctctggca gtgcgagttg gcgcggaaga tgcgcagaag gagcatcgtg cccgtgcgga
    24961 tgggttccag cgtgctcacg tagctctgca gctccctgcg acggatttcg ggagccgtgt
    25021 tcatccagag gtgcatctgc ggcaggtcga attcgcaggt gccgcctgca atgctctggc
    25081 gcgtgcggat gatctgcaac cattggtttt cacgcacgct ctgcgtcgtg cgtccgacag
    25141 aatccgaaac ggcctgaatg gctgcgccga tctcggcgag atgagcggcc atgtcgggat
    25201 ccgccgaggc gttcttggca atgaggcgct ggcgctggcg ttcgagctcc tgaatgagtt
    25261 cgtggcgcag gtccgagcgg gcggtggcgt caagcaggtc gaagagggcg gatatgccgg
    25321 cctgatgcgc aatggcgctc gtctgctcgc aaaaccagtc gaagcgctgg aagagcgcct
    25381 ccaaccggag atacgtgcgg attttctcat tgcaggggaa ttcgtagaga agatgctcgg
    25441 ccgtggacat gtctgcttac gctccaagaa ggtggacgct gaccctaaaa ataccacagc
    25501 ccttcggact gcggtcctcg ttcatcgggt aattgccatc cagatgcggc tgagcgcgtc
    25561 aaggcgcttg cggagcgccg cttcgtcgtc ctcgttgacg atgaggtcgt cggcgcactg
    25621 cagaatccgg cttcgcggag gctgagcctt catcatcagg cgggcggtct cctcggtgag
    25681 tccgggacgg gagacgatgc gcgagagacg aacgtcgtcc gaggcgtcga tgaccagaac
    25741 gcggctgacg gcaggatggc ggaagtcgtc gcgccagaga agcgggcagt cgtagatgac
    25801 gattggagac gcagagaggg actcgaactc gcgtgtaacg gcagcgccga tgaggggatg
    25861 gaggcaggcc tcgaggcgct tgagtgcgcc tgcgtcccga aaggcaagtt cacgcatgcg
    25921 gcgcctgtcc atggcgccct cgggggtgag aaattccgga ccgaaggctt ccgcgacggc
    25981 gagcgagcct gctgcacccg ggcctgtgag cgcgcgggag atggcgtccg cgtcgacgac
    26041 ggggaggccc gatgcgcgaa ggtgtgcgga tgcggtgctt tttcccgcgc cgatgccgcc
    26101 ggtgagtccg atgatgagtg acatgggatg atctcgccga aaagcagaaa ggcaccgtcc
    26161 gaagacagtg cctttctgtg gaaacctggt cggggtgaga ggattcgaac ctccgactcc
    26221 tacgtcccga acgtagtact ctaccaggct gagctacacc ccgaattgtc ttgacagcgt
    26281 attccaactg tcgaggaaga gcagtatgcc agaaaaattc ccaaaagtaa agaccactcc
    26341 gaaaattatt cggcttcttc cgaatgaagt ggtcggggtg agaggattcg aacctccgac
    26401 tcctacgtcc cgaacgtagt actctaccag gctgagctac accccgactt tcctcacaaa
    26461 tcaacgatca cgctgaaccg tgaaggcgag caattctagc agagaatccc tgaaaatgga
    26521 aggggcgaga ttttcaagcg ctgcgcgccc cctgtcgagc tccttctcgg cgcgcaggcg
    26581 gcacttctca agcgcatcgg acttcagcac gatggcggcg atcgtcggga agtcgccgtc
    26641 gccgaggcgg atggcttcgt cgatcatggc gcgctcctcg ggcgtgcagg attcgcgggc
    26701 gtagatgatg ggcagggtca ccttgccctc gcgcaggtcg gcaccgaggt tcttgcccgt
    26761 gtcgacggcg ttgccgcggt agtcgagcac gtcgtcggcg atctggaagg cgttgccgag
    26821 ggcgagcgca tattcggcga cggcctcttc cttttcagga ccgcagtccg caatggcggc
    26881 ggccatgcgt gcgcccgctt cgaaaaggca ggcggtcttg cgctcaatga cgcggaagta
    26941 gcgggtttcg tccacgctgg ggtcgtgtgc attgaccatc tgcaccactt cgccttcgct
    27001 caggcggttg gcggcgtcgg aaagagcctc catgacgcgc aggtttccag ccttcaccat
    27061 catctggaag gagcgggtgt agaggaagtc gcccacgagc acggccgcgc cgttgcccca
    27121 gcggctgttg gccgtcgggc ggccgcggcg catgtcgctt tcatccacga cgtcgtcgtg
    27181 catcagggtt gccgtatgca gaagctcgat cgtggcgccg agatactggt gcagcgtgcc
    27241 ctcgtagccg agcgcacggc agatgagcat cagaagggcg gggcgcatgc gcttgccgcc
    27301 cgcactcgtg atgtattcgc tgatttcctt catgcggggc acgtcgctgg ccagttcgct
    27361 gcgaatgacc gcatcgacgg cggccatgtc ttcggcgatg ggggcgagaa ccttcttgat
    27421 gagggccttg ggatcctgtt ggttttgcat atcggacatc tgtcttgaaa aagcagttga
    27481 gtgctgattc tggaagtata gccaagcgga aagccttccg aagaaggcgg aagatcggcc
    27541 ggaagcggcc ttcagccccc gttcccgcag gggcgagaag aaaatcgctg cttttgcttg
    27601 actgaatgga aggagcatgc aataatcacg agctttccac gcaaccaaac gtgagttggc
    27661 gtcatttggg gaatgtctcc tcaagcgcgc tcacgcgatt acaaaaaggt tctgcagaat
    27721 ccggccgcat caggcggacg gcgaagcgga acgggcgagg tttattacat gtacgctatt
    27781 atcaagactg gcggtaagca gtaccgcgtt tccgtcggcg acaagctcaa ggttgaaacc
    27841 cttgctgttg aagccggcgc tcaggtgacc ctcaccgaag ttctcgccgt cagcggcgaa
    27901 gaaggcctca aggtcggcaa tccgacgatc gaaggcgcta cggtcaccgc gaccgtcctt
    27961 tcccacggcc gtggcgaaaa ggtccgcatc ttcaagttcc gccgccgcaa gcactccatg
    28021 aagtctgccg gccatcgtca gaactacacc gagctcaaga tcgaagcgat cgcggcttaa
    28081 tttgcaaccg actttttata gagaagtact aaaatggcac acaagaaagg tggcggctct
    28141 acccgcaacg gtcgtgactc caaggccaag cgtcttggcg tgaaggtttt cggtgacgct
    28201 gctgtcaacg ccggcggcat catcattcgt cagcgtggca cgaagttcca cgccggcaac
    28261 aacgtcggca tgggcaagga ccacacgctt tacgccctcg ttgacggcgt cgtgaagttc
    28321 gaagtcaagg gtgctttcaa ccgtcagtac gtgaaggttg aacccatcgc tgcctaattg
    28381 agggcaggcg tcctcaggga cgcggacgac agaagaaaag cccagtgcgg atcctccggc
    28441 tgggctttct tgttgtctga agcatcggct gcgtaaatat ccagaaacgc gccttcgcat
    28501 gcgcattcga gcctgccgat ctgctattgt ggcactcgcg caagtgcgaa tgcgaaagca
    28561 cggcattccg aaactgcagg aacctcttcc attggttctg ttgtcaaaat atcctctgcg
    28621 caacagggag tgttcgcaga gactcaactt cagaaggtgt actcgtgaag tttgttgatg
    28681 aagcaaaaat tgaagtccag ggcggcaagg gcggcaacgg tgcggcaagc ttccgccgcg
    28741 aaaagttcat tccgaagggt ggtcccgacg gcggtgatgg cggccgaggc ggctccgtct
    28801 acgcggttgc cgaccgcaac atcaataccc tcgtggacta tcgctacacg cgcaagttct
    28861 tcgcccccaa cggcgagaac ggccgcggcg cggactgcta tggcgcaggc ggcgacgaca
    28921 tcgttctgcg catgcccgtg ggcactcttg tgcgcgacac ggataccggc gaggtgattg
    28981 ccgacctgaa cgttcacggc gctcgccagc tgctcgctgc gggcggcaag ggcggtctcg
    29041 gcaaccttca cttcaagagc tcgatcaatc gtgcgcccaa gcaggcgact ccgggcgaac
    29101 ccggtcagta ccgttcgctc gagcttgagc ttcaggtgct cgccgacgtc ggtcttctcg
    29161 gcatgcccaa cgcaggcaag tcgaccttca ttacggccgt ctccaacgcc cgtcccaaga
    29221 tcgccgacta tccgttcacg acgctccatc cgcatctcgg cgtggttcgt gtcggtcccg
    29281 agcagagctt cgtgctcgcc gacgtgcccg gcctcatcga aggtgctgcc gaaggcgcag
    29341 gtctcggtca tctgttcctc cgtcatcttt cccgcacgaa gattctgctt cacatgatcg
    29401 acgccgctcc cttcaatgag gagacggatc ccgttcagga agccaaggct ctggtgctgg
    29461 agcttgaaaa gtatgatccg gcgctcgccg caaagccccg ctggatcgtg ctcaacaaga
    29521 tcgacctcgt cgaggaggct gaccgcgacg ccctcatcga gcgctaccgt tccgagctgg
    29581 ccgccaacgg cgaacccatc ttcgtcatct ccgctgcgac ccgtcagggc tgcgaagagc
    29641 ttgtgaaggc tctcgcacag cgcatcgatg acgatcgccg cgaagaggtc gcgttcgccg
    29701 acgacacccg cttcgatcct gccgaacact aagaaacggc tcatttccgg aagagactcg
    29761 tccgctgctg cgggccgggt ctctttgttt ttcccgcagg ccggatacgg cgggcggttt
    29821 cctctcaatt cactgatcga acatcatgca agcatcagtc gttgaacgtc tcttcagcct
    29881 caaggagaac ggcacttcgg tttctcagga agtctctgcc ggcgtcacca ccttcatggc
    29941 gatgagctat ctcatattcg tcattccctc catgctcgcc gacggcggca ttccccgcga
    30001 tgcggccacg agcagcgtga ttctcgtcac ggtcgcggtg accctgttga tgggcctgtg
    30061 ggcccgatac cccgtgggcg tggcccccgg cctcggcatt acggcgttct tcgcctacta
    30121 tgtctgcggt cccgccggct tcacctggca ggcgggtctt ggtgcggtgt tcatttccgg
    30181 cctcgtcttc ttcctgctca cggtgacgcg catccgccag ttcatcgtga acgccgttcc
    30241 gatcgacctc aagctctcca tcgtggtggg catcggtgcc ttcattgcgc tcatcggtat
    30301 gaagagcgcc ggtctggtca ttgccgaccc gagtaccttc gtggcgctcg gcaacgtgac
    30361 tcagcccgag gttcagctct ccgtcttctg cttcttcctc acggctgcgc tgatggtgaa
    30421 gggtttcagg gccgccatgc tcatcggcat tcttgcgacg acggcgctcg gcgtcattct
    30481 tggcgtgacg gagcttccga agggcgcctg ggtgagcctc acgcttcccg acatctccgc
    30541 cgtcttcatg cagatggacc ttcagggcgc gctttcccat ggccttctca gcatcatctt
    30601 cacgctgacg atggtcgacc tcttcgacaa catgggcgtg ttgatcggtc tggcccgcaa
    30661 gtgcggcttc atgaggcccg acggggagat ccgcaacctc gacaaggcgt tcatcaccga
    30721 ctccatcggc acgatgttct ccgcctgcat gggcacgacg accgccacct cctacctcga
    30781 aagcgcttcg ggcgtcgctg cgggcggccg tacgggcctg accgccgtca cgacggccgg
    30841 ctgcttcgtg cttgcgctct tcttcattcc gcttgtgggc gtcgtgcccg cgtatgcgac
    30901 cgctccgatc ctcatcatcg tgggtgctct catgatgcag gaggtcgtgc acatccgctt
    30961 cgaaaggctt gaaatcgcga ttcccgcctt cctgaccatc atgacgatgc ccttcacgtt
    31021 caacatcgcc acgggcttcg gcttcggctt tgtgagctac acggtcatcc acgtgctgat
    31081 gggtcgctgg cgcgaggtca cgcccttcat gtacctgatc tccgcctgct ttgtagcgaa
    31141 cttcatcatg agaacctgat cccgatcagt cgtccccttg ggacaaatcc acgcctttgc
    31201 cagatgcgat ccggtcctga cggacgccct tcggcatgag gatgggagag gcccgtgaat
    31261 tgcttaattc acgggcctct ctgcatactc atggcaagaa tcccctcaag ttcccttgcc
    31321 gcaggcgagg attccgttcg ctcgaactcg gatgttcgga ctcgaaagga gcggtctgca
    31381 tgaggttgga tgcaacgggc gggagggggc ggtgaaggag agggcacggc agaatttctt
    31441 gctttgcgct atgctcaaag atcccgttca atcctttcca aaggctttat ccatggaatc
    31501 acttctcaag aacgcgcacc gggtcgtcgt caaggtcggc agcgcgctcg tcaccaacga
    31561 cggtcgcggt ctcgacattc aggccatcga acgctgggca acccagattg cggaactgca
    31621 gaagatgggc cgcgaggtca tcctcgtgag ttcgggcgcc attgctgaag gagtcctgcg
    31681 ccttggctgg gaaaagcgtc cttcgcatgt ccaccagctg caggcggctg cggccgtcgg
    31741 tcagatgggc ttggtcgaag cctatgaacg gcatttccgc aatcatggca tcggcaccgc
    31801 ccaggtgctt ctgacgcacc agaatctgac ggaccgcgag cagtatctca atgcacgaat
    31861 gacgatcagg gaactcctga gactcggcat cgtgcccgtc atcaacgaga acgacaccgt
    31921 tgtgacgagt gaaatcaagg tgggcgacaa cgacacgctc ggcgccctcg tgaccaacct
    31981 cgttgaagca gacgtgctcg tcattctcac cgaccagcag gggctttaca cggccgatcc
    32041 gcgcaaggat cccgaggcga tgttcgtcag cgtcgcgact gccggcgatc ccgtgcttga
    32101 aaagatggcg ggcggtgcag ccagctccct ttccaagggc ggcatgatca cgaagattct
    32161 cgcggccaag cgcgccgctc gatccggtgc gggcacgatc atcgcatcgg gtcgtgagac
    32221 ggacgtgctc acccgtctgg ccagaggcga gctcatcggc acccatcttc gtccctcctc
    32281 caccgtcctg catgcccgca agcagtggat tgcagaccat ctccgcgcct ccggcagcct
    32341 tgtgcttgac gaaggggcgg ccaaggccct cgttgccaag cacacctcgc tcctgcccgt
    32401 gggcgtcatc ggcgtcaagg gcgagttcgt gcgtggcgag gtcgtctcgt gtctcaccga
    32461 ggacggatgt gaaatcgccc gcggtctcgt gaactattcg agcgacgatg cgtcccgtat
    32521 tctgcgcgca cacacggagg agatcgagga gcgcctcggc tacatggaac agcccgagat
    32581 gattcatcgt gacaacatgg tggtgctcgc aggcgaatga cctcgcccgc atggccgctg
    32641 cggcctcaaa acgcgccttt cgggttagcg ttcagagggg ctggcggtta aaataaacag
    32701 attgccttta ttcaagcggc ttcccgacgt ctgacggaag ccgccgttat tttcaacaga
    32761 cacacgttaa gaaaaaatgc gcgcccgttc ctatttcctt tccacgctga aagaagcgcc
    32821 tgccgatgct gatgtgatca gccagcagta catgatccgt gccggcatga tcaagaagct
    32881 tgccgccggc gtttacacct acatgccggt cggcctgcgc acgatccgca agatcgagaa
    32941 gatcatccgc gaggaaatgg accgtgcggg cgcgatcgaa ctcctgatgc cgctcgtgca
    33001 gcctgccgag ctctggcagg agtcgggccg ctgggaaaag tacggtgccg aacttctccg
    33061 cttcaaggac cgtcatgacc gcgactttgt gattcagccg acttccgaag aagtcgtcac
    33121 tgacatcgcc cgtcaggaaa tcaagagctg gcgtcagctt cccgtgaact tctaccacat
    33181 ccagacgaag ttccgcgacg agcgtcgccc ccgcttcggc gtcatgcgcg gccgcgagtt
    33241 cacgatgaag gatgcctact ccttcgaccg tgacgccgag ggcgccgccg tttcctacga
    33301 caagatgtac caggcctatc agcgcatctt cacgcgcctc ggcctcatgt tccgcgccgt
    33361 gcgcgccgac accggcgcga tcggcggcag ccgctcccac gagttccagg tcatcgccaa
    33421 tgccggtgaa gacgtcatcg cctactgccc cgatagcgac tacgccgcca acatcgagct
    33481 ggccgaggcc cgttcgctca ttgacgtccg cgccgccgct gctgaagaga tggtgaagcg
    33541 tccgaccccg ggcaaggaaa agtgccacga cgtggccgaa ttcctcggta ttccctttga
    33601 aaagtccgtc aagagcgtcg tgcttgccgc cgaccgcact gacgaaaagg gcaatccgct
    33661 tcccgccaag atcgtcctca tcctgctccg tgccgaccac gacctcaacg aagtcaaggc
    33721 cggtcatctg cctgagctca aggacggctt ccgctt
//
LOCUS       DBMDTI010000005        24010 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_732_length_24010_cov_4.085073, whole genome shotgun sequence.
ACCESSION   DBMDTI010000005 DBMDTI010000000
VERSION     DBMDTI010000005.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 24010)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24010)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24010
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_732_length_24010_cov_4.085073"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="ACZPB6_00800"
     CDS             <1..159
                     /locus_tag="ACZPB6_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR1183049.1"
                     /translation="ELPEGVEMVMPGDNIQMTVKLICPIAMEQGLRFAIREGGHTVGA
                     GVVAAVIE"
     gene            261..572
                     /gene="rpsJ"
                     /locus_tag="ACZPB6_00805"
     CDS             261..572
                     /gene="rpsJ"
                     /locus_tag="ACZPB6_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR1183050.1"
                     /translation="MQSQRIRIRLKAYDYKLIDQSALEIVDTAKRTGAVVRGPVPLPT
                     RIQRFDILRSPHVNKTSRDQLEIRTHQRLMDIVDPTDKTVDALMKLDLPAGVDVKIKV
                     Q"
     gene            745..1389
                     /gene="rplC"
                     /locus_tag="ACZPB6_00810"
     CDS             745..1389
                     /gene="rplC"
                     /locus_tag="ACZPB6_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR1183051.1"
                     /translation="MSDKLGIVGRKIGMTRIFTEDGVSVPVTVLDVSNNRVTMVKTVE
                     TDGYNAIQVAFGSKKPTRVSKPLAGHFAKAGVEAGSMLKEFHIEAEQVAEFKPGMALK
                     AEMFTEGQKVDVTGTTIGKGFQGGIKRHHFSSNRASHGNSVTTRAPGSIGNRQDPGRV
                     FPGKRMAGHLGDVQRTTQNLIIARVDTERQLLLVRGAVPGAKGGEVIVRPAVKA"
     gene            1401..2021
                     /gene="rplD"
                     /locus_tag="ACZPB6_00815"
     CDS             1401..2021
                     /gene="rplD"
                     /locus_tag="ACZPB6_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR1183052.1"
                     /translation="MELNFLNEQGKTVDVNEAVFGREYNEALVHQIVVAFQANARLGT
                     RAQLTREAVHHSTKKPWRQKGTGRARSGMTSSPVWRGGGRAFPNSPDENFSQKVNKKM
                     YRAAMASIWSKLVADERLCVVESFAVESPKTKAFATQLKTMGLQNKVLLVVGAEELTD
                     NLILASRNMKDVLVVPTRYADPLCLLYFDKVVVTKAAVSEIEEMLA"
     gene            2018..2317
                     /gene="rplW"
                     /locus_tag="ACZPB6_00820"
     CDS             2018..2317
                     /gene="rplW"
                     /locus_tag="ACZPB6_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR1183053.1"
                     /translation="MNQERLLKVLVAPVISEKSTMVAEKLNQACFVVVPDANKAEVKA
                     AVELLFKVQVKSVQILNLKGKQKRFGRFMGKRNDVRKAYVTLAQGQEINFAQEVK"
     gene            2317..3144
                     /gene="rplB"
                     /locus_tag="ACZPB6_00825"
     CDS             2317..3144
                     /gene="rplB"
                     /locus_tag="ACZPB6_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR1183054.1"
                     /translation="MALVKLKPTSAGRRHAVKVVNKELHKGKPWAALTEKKNRGSGRN
                     NNGHITCRHKGGGAKRAYRIIDFKRQKDGVPARVERIEYDPNRSAHIALVCYADGERC
                     YIIAPKGLTVGQEIMNGPESPIKVGNTLPLRNIPVGSTIHCIEMLPGKGAQMVRAAGA
                     FAQLVAREGEYAQIRLRSGEVRRVLIECRATIGTVGNEENSLRELGKAGAKRWRGVRP
                     TVRGVAMNPVDHPHGGGEGKTGTGRAPVTPWGQLTKGYRTRNCKRTDNMIVSRRNRK"
     gene            3155..3433
                     /gene="rpsS"
                     /locus_tag="ACZPB6_00830"
     CDS             3155..3433
                     /gene="rpsS"
                     /locus_tag="ACZPB6_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR1183055.1"
                     /translation="MSRSLKKGPFVDGHLMKKVEAAQASRDKRPLKTWSRRSTILPEF
                     IGLTIAVHNGRQHVPVYINENMVGHKLGEFANTRTFKGHAAGDKKVGK"
     gene            3434..3775
                     /gene="rplV"
                     /locus_tag="ACZPB6_00835"
     CDS             3434..3775
                     /gene="rplV"
                     /locus_tag="ACZPB6_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR1183056.1"
                     /translation="METTAIVRGVRLSAQKGRLVADLVRGKPVDKALAILTFTQKKAA
                     VIIKKALESAIANAEHNDGADIDTLVVTKIHVEKAATLRRFAARAKGRGTRINKQTSH
                     IYISVGDAKAK"
     gene            3785..4561
                     /gene="rpsC"
                     /locus_tag="ACZPB6_00840"
     CDS             3785..4561
                     /gene="rpsC"
                     /locus_tag="ACZPB6_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR1183057.1"
                     /translation="MGQKINPTGFRLPVTRNWTSRWYAVGRDFSRTLGEDLAVRSFLL
                     KKLRNASVSRILIERPANNARITIFSARPGIVIGKQGADIEALKAEVQKMMGVPVHVN
                     IEEVRRPELDAQLIADGITQQLEKRVMFRRAMKRAMQNAMRLGAQGIKIMSSGRLNGA
                     DIARCEWYREGRVPLQTLRANIGYGFSEALTTYGIVGVKVWVYKGDNFGQEEAVEAPR
                     EDRRGRRPGDRAGKPGARRNNGRRNDKQQQARKPVAKDGE"
     gene            4564..4980
                     /gene="rplP"
                     /locus_tag="ACZPB6_00845"
     CDS             4564..4980
                     /gene="rplP"
                     /locus_tag="ACZPB6_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR1183058.1"
                     /translation="MLQPNRRKYRKDQKGRNYGLATRGANVSFGEFGLKTLERGRITA
                     RQIEAARRAITRHIKRGGRVWIRVFPDKPISSKPAEVRMGNGKGNPEYWVALVQPGRV
                     LYEMDGVDEQLAREAFALAAAKLPVKTTFVVRQIGM"
     gene            4991..5185
                     /gene="rpmC"
                     /locus_tag="ACZPB6_00850"
     CDS             4991..5185
                     /gene="rpmC"
                     /locus_tag="ACZPB6_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR1183059.1"
                     /translation="MNAKELRAKDEAALKQELNDLQNTHFKLRMQKATQQLTNTSSLR
                     TTRRDIARVRTILTEKATTK"
     gene            5182..5448
                     /gene="rpsQ"
                     /locus_tag="ACZPB6_00855"
     CDS             5182..5448
                     /gene="rpsQ"
                     /locus_tag="ACZPB6_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR1183060.1"
                     /translation="MTETAQSLNRTLVGTVSSSKMEKTVTVLVERKVKHPLYGKYVVK
                     SKKYHAHDEIGCNEGDKVEIAECRPLSKTVSWTVTKVLEKAVVL"
     gene            5694..6062
                     /gene="rplN"
                     /locus_tag="ACZPB6_00860"
     CDS             5694..6062
                     /gene="rplN"
                     /locus_tag="ACZPB6_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR1183061.1"
                     /translation="MIQMQSRLDVADNTGARSVMCIKVLGGSKRRYANIGDVIKVTVK
                     EAAPRGRVKKGEVYNAVVVRTAHGVRRPDGSSINFDGNAAVLLNSKLEPIGTRIFGPV
                     TRELRTEQFMKIVSLAPEVL"
     gene            6076..6396
                     /gene="rplX"
                     /locus_tag="ACZPB6_00865"
     CDS             6076..6396
                     /gene="rplX"
                     /locus_tag="ACZPB6_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR1183062.1"
                     /translation="MQRIRKGDEVVVIAGRDKGTKGTVLSRVDDSHVIVEGVNVVKKH
                     VRPNPHLGVAGGIVEKNMPIDQSNVMLVNPETGKGDRVGFKEVDGKKVRVFKSNGAVV
                     GAKA"
     gene            6408..6947
                     /gene="rplE"
                     /locus_tag="ACZPB6_00870"
     CDS             6408..6947
                     /gene="rplE"
                     /locus_tag="ACZPB6_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR1183063.1"
                     /translation="MSRFSSMYHDTIVPELMKKFGYKTTMQVPRITKITLNMGVGEAV
                     NDKKLVDAAVGDMTKIAGQKPMVTRTRKAIANFKIRENMPIGCMVTLRGERMYDFLDR
                     LVTVAFPRVRDFRGVSGRAFDGRGNYNIGLKEQIIFPEIEYDKIDRLRGMNISITTTA
                     KSDEEAKALLAGFSFPFRN"
     gene            6960..7265
                     /gene="rpsN"
                     /locus_tag="ACZPB6_00875"
     CDS             6960..7265
                     /gene="rpsN"
                     /locus_tag="ACZPB6_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIR1183064.1"
                     /translation="MAKLALINREAKRAATVAKYAAKRAALKAIINDATRSMEERLEA
                     RAKLQALPRDASPVRQRNRCALTGRPRGTFRKFGLCRGMIRDAAMRGDIPGLVKASW"
     gene            7283..7678
                     /gene="rpsH"
                     /locus_tag="ACZPB6_00880"
     CDS             7283..7678
                     /gene="rpsH"
                     /locus_tag="ACZPB6_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR1183065.1"
                     /translation="MSMSDPIADMLTRIRNGQIVEKAEVVMPSSKLKVAIAKVLKDEG
                     YIDGYTVVANEGKPELHVGLKYYAGRPVIERLERVSRPGLRIYKNHGSIPQVMNGLGI
                     AIVSTPKGVMTDRAARSAGIGGEVLCYVA"
     gene            7693..8226
                     /gene="rplF"
                     /locus_tag="ACZPB6_00885"
     CDS             7693..8226
                     /gene="rplF"
                     /locus_tag="ACZPB6_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR1183066.1"
                     /translation="MSRIAKIPVQIVQGVSYTLTADNITVKGPQGEVSMHILPFVTIT
                     EEDGALHFKQVEETREANANVGTMRALVADMVKGCSVGFQKKLQLVGVGYRAQAQGDK
                     LNLSLGFSHPVVHQMPAGVKCETPSQTEILIKGADKQKVGQTAAVIRSYRAPEPYKGK
                     GVRYADEVVVIKETKKK"
     gene            8245..8610
                     /gene="rplR"
                     /locus_tag="ACZPB6_00890"
     CDS             8245..8610
                     /gene="rplR"
                     /locus_tag="ACZPB6_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR1183067.1"
                     /translation="MNKKQSRLRRARATRARILLQKVDRLTVHRTNLHIYASIISADN
                     GRTLVSASTVEPEVRAQLANVSGRGGNVAAAKLVGTRLAEKAKAAGIESCAFDRSGYR
                     YHGRVAALAEAAREAGLKF"
     gene            8622..9143
                     /gene="rpsE"
                     /locus_tag="ACZPB6_00895"
     CDS             8622..9143
                     /gene="rpsE"
                     /locus_tag="ACZPB6_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR1183068.1"
                     /translation="MAKAQGKNQMVDEQGDGLCEKMIAVNRVTKVVKGGRILAFAALT
                     VVGDCDGRVGMGTGKSREVPQSVSKAMDRARHDMKRFPLKNGTIHHAVEGRHGASRVI
                     MMPAPEGTGVIAGGPMRAVFEAVGIRNVVAKAYGSTNPYNLVRATIQALNNLRSPAEI
                     AAKRGKTVEELLG"
     gene            9159..9341
                     /gene="rpmD"
                     /locus_tag="ACZPB6_00900"
     CDS             9159..9341
                     /gene="rpmD"
                     /locus_tag="ACZPB6_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR1183069.1"
                     /translation="MSEKKTVKVTLVKSPIGTKADHRATLAGLGLKKIRQSRVLEDTP
                     AVRGMINKVAYLVRAE"
     gene            9358..9798
                     /gene="rplO"
                     /locus_tag="ACZPB6_00905"
     CDS             9358..9798
                     /gene="rplO"
                     /locus_tag="ACZPB6_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR1183070.1"
                     /translation="MRLNTIKPAEGSKHAHHRVGRGVGSGWGKTAGRGHKGQKSRAGG
                     FHKVGFEGGQMPMYRRLPKRGFTSLTRKYCEVVRLSDLERLSCDEIDLAVLKAAGVVS
                     ALAQNARVILAGEITRKVTVRGLGVTKGARAAIEAAGGSIADNE"
     gene            9825..11156
                     /gene="secY"
                     /locus_tag="ACZPB6_00910"
     CDS             9825..11156
                     /gene="secY"
                     /locus_tag="ACZPB6_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR1183071.1"
                     /translation="MATSPSSKQQSGLSKYGDLKGRLIFLALALVVYRIGAHVPVPGI
                     DPDMLKQLFDEQQGGILGLFNMFSGGALSRFSVFALGIMPYISASIIMQMLSYVLPEL
                     EALRKEGESGRRKITQYTRYGTLLLGLFQGFGIAVALESQAGLVLDPGFMFRITTTIS
                     LLTGTMFLMWLGEQITERGLGNGISIIIFAGIVAGLPSGTSGLFQLVSTGAISPLAAI
                     FVIAIILAVTAFVVFIERGQRRITVNYAKRQIGNKIYGGQSSYLPLKMNMSGVIPPIF
                     ASSLILFPATLAGWFTSGDSTRWIRDLAAALSPGQPLYTLLYAVLIVFFAYFYTALVF
                     NPKETAENLKKSGAFIPGIRPGDQTARYIDRVLLRLTLGGAVYLVFVCLVPEFLNLRF
                     NVPFYFGGTSLLIVVVVTMDFMSQVQAYLMTHQYESLLRKTNFRGLGQPKH"
     gene            11413..11526
                     /gene="rpmJ"
                     /locus_tag="ACZPB6_00915"
     CDS             11413..11526
                     /gene="rpmJ"
                     /locus_tag="ACZPB6_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR1183072.1"
                     /translation="MKVNASVKKMCRKCKIIKRKGVVRVICADPRHKQRQG"
     gene            11538..11903
                     /gene="rpsM"
                     /locus_tag="ACZPB6_00920"
     CDS             11538..11903
                     /gene="rpsM"
                     /locus_tag="ACZPB6_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR1183073.1"
                     /translation="MARIAGINIPPQQHAEIGLTAIYGIGRSRAREILTNCGIEFSKK
                     VKDLTDADLEKIREQVGKFTVEGDLRREIQLSIKRLIDLGTYRGMRHRKGLPCRGQRT
                     RTNARTRKGPRKAGVTLKK"
     gene            11916..12323
                     /gene="rpsK"
                     /locus_tag="ACZPB6_00925"
     CDS             11916..12323
                     /gene="rpsK"
                     /locus_tag="ACZPB6_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR1183074.1"
                     /translation="MAGNNSKQAAAQRARKKVKKVVTEGIAHVHASFNNTIITITDRQ
                     GNAISASSSGAQGFKGSRKSTPFAAQVAADVAGKAALEYGLKNVEVRIWGPGPGRESS
                     IRALNALGIRVTSIQDVTPIPHNGVRPSKRRRV"
     gene            12419..13045
                     /gene="rpsD"
                     /locus_tag="ACZPB6_00930"
     CDS             12419..13045
                     /gene="rpsD"
                     /locus_tag="ACZPB6_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR1183075.1"
                     /translation="MARYLGPKLKLSRREGIDLNLKSARRSFESKVKDAGSKPGQHGK
                     ISGARTSDYGAQMRETQKAKRLYGVLERQFRRYFAEAERITGNTGENLLALLESRLDN
                     VVYRMGFGSTRAEARQVVSHGAILVNGKKVNIPSYHVKPGDVISVREKAQKQARIAEA
                     LELAQQNGFPGWVSVEPKKFEGTFKNVPARDEIAYEINEALIVERYSR"
     gene            13325..14323
                     /locus_tag="ACZPB6_00935"
     CDS             13325..14323
                     /locus_tag="ACZPB6_00935"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR1183076.1"
                     /translation="MANTSLLKPTSVEVALSENNPNRAVITLEPFERGYGHTLGNALR
                     RILLSSMIGFAPTEVQITGIVHEYSQIDGVLEDVVDILLNLKGVVFKLDGRDEVTVML
                     RKDGEGVVTAADFDLPHDVSVVNPDHVIAHLSGGRLEMQVKIEKGRGYQPGDVRAFRD
                     DYSKAAIGRIIMDASFSPILRVAYQVQAARVAQRTDLDKLEMTIETNGAIGPEDALRE
                     AVRILQDQLAVFGMIHQDTGADAAPAEEDEDVPPPLDPILLRPVDDLELTVRSANCLK
                     AENIYYIGDLIQRTENELLKTPNLGRKSLNEIKEELAAHGLTLGSRLENWPPAQEH"
     gene            14565..14954
                     /gene="rplQ"
                     /locus_tag="ACZPB6_00940"
     CDS             14565..14954
                     /gene="rplQ"
                     /locus_tag="ACZPB6_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR1183077.1"
                     /translation="MRHRLGLRKLNRTSAHRLAMLRNMMCSLIKHEAIKTTLPKAKEL
                     RRVVEPMITLAKEPTVANKRLAFNRLRDREVVCKLFNEIGPRFANRNGGYTRILKMGF
                     RVGDNAPMAYMELTEKAVAVEEVKSEE"
     gene            15041..15418
                     /locus_tag="ACZPB6_00945"
     CDS             15041..15418
                     /locus_tag="ACZPB6_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1183078.1"
                     /translation="MRIDKWLWAARFFKTRTIAQEEVGLGRVLIDGQRMKASRDVRVG
                     DRLTIRRGEETFHVFVEGLSNTRGPAAEAQKLYRETEESKMTRAQMKEMLRLASEPAS
                     YIRQGRPTKKDGRLLREWQHKMK"
     gene            complement(15497..16510)
                     /gene="hemB"
                     /locus_tag="ACZPB6_00950"
     CDS             complement(15497..16510)
                     /gene="hemB"
                     /locus_tag="ACZPB6_00950"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR1183079.1"
                     /translation="MQTLGAYPSVRMRRMRHDDFSRRLMRENVVTPNDLILPVFVIEG
                     TGLREPVPSMPGVDRLSIDELLKVAGECVELGIPMIALFPHIEEALKTPDGCEAANPD
                     GLIPRAVKALKAAYPQLGVMTDVALDPYTTHGQDGLIDEEGYILNDETIEMLVQQVLA
                     QARAGADVVAPSDMMDGRIGIIRKRLEDEGLIHTRIMAYSAKYASSYYGPFRDAVGSS
                     SNLGKSNKAVYQQDPANGNEALWEVGLDLAEGADMVMVKPGVPYLDVLWRVKQEFKAP
                     TFAYHVSGEYAMIKFAAQAGAIDEKKITFETMVCFKRAGADGILTYCALDVARWISEG
                     YNY"
     gene            complement(16579..17226)
                     /gene="yihA"
                     /locus_tag="ACZPB6_00955"
     CDS             complement(16579..17226)
                     /gene="yihA"
                     /locus_tag="ACZPB6_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR1183080.1"
                     /translation="MSIFDTARFVISAAKLSGLPPEAVPEIAFAGRSNAGKSTTINVL
                     TRKTRLAFASKTPGRTQLINFFALSKKNEAGVREDVGCLVDLPGYGFAKASEDTRKTW
                     SQLVGGYIAQRPNMVGLVIVMDARRPFMPTDEWLIEFMAGRPNVRMHWLLNKADQLKN
                     QERRTTLRNAQKRAAEIGPHVTVQLFSGMKKEGTEELGAVLEGWLGLNAQESPKE"
     gene            complement(17340..18383)
                     /locus_tag="ACZPB6_00960"
     CDS             complement(17340..18383)
                     /locus_tag="ACZPB6_00960"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HIR1183081.1"
                     /translation="MHLENSVKLDFKDVLIRPKRSTLTSRSEVDITREFKFLHSSEPY
                     HGIPIIAANMDTTGTFEMASALGRHKMSTALHKHYTAKEYVDFFQKLENKSDAFYSLG
                     IGEHDWQKFREVMAAAPGAIRYVCIDVANGYTEAFVAFVKKVRETYPDIVLMAGNVVT
                     GDMTEELLLAGADIVKVGIGPGSVCTTRKMTGVGYPQLSAIIECADAAHGLGGRICAD
                     GGCTVPGDIAKAFGGGADFVMLGGMLAGHDEAAGDVVTTEDGVFKRFYGMSSRNAMDK
                     YAGGVAKYRAAEGKAVLIPARGPVESTIQDILGGVRSACTYVGARRLKELTMRTTFVR
                     VSMQLNEVFGKAP"
     gene            complement(18544..19824)
                     /gene="lysA"
                     /locus_tag="ACZPB6_00965"
     CDS             complement(18544..19824)
                     /gene="lysA"
                     /locus_tag="ACZPB6_00965"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR1183082.1"
                     /translation="MSENNHRPCFHREDGVLFCEGVSLLELSRRLEQTTYVYSRARIE
                     RNYLAYEEALADVPHAVCYAMKANSTAGVLQVLARLGAGVDTVSGGEIARALRAGFRP
                     EKIVYSGVGKTAREITFALKAGIGCFNVESEPELERINRIATELGVRAPISIRCNPDV
                     DPKTHPYISTGLKNNKFGIAIADVEDIYLRAANMPGIEVKGIDAHIGSQITEIQPYLD
                     SVHRLLDTMSALRDKGLVLHHLDMGGGLGIQYRPEDQPPTPQTLLRPVRRMMEERGFG
                     DAVLMVEPGRSIVGDAGLMLTQVEYIKQSETRNFCIVDGAMTDLIRPALYSAWMDIVP
                     VVERHDAAPLVMDVVGPVCESSDFLGRARELAVREGDWIAVCDAGAYGASMASRYNSR
                     MLPMEVMIDGERVLPLRTPDTFDEMVSGEVLLEL"
     gene            complement(19871..20008)
                     /gene="lptM"
                     /locus_tag="ACZPB6_00970"
                     /gene_synonym="yifL"
     CDS             complement(19871..20008)
                     /gene="lptM"
                     /locus_tag="ACZPB6_00970"
                     /gene_synonym="yifL"
                     /inference="COORDINATES: protein motif:HMM:NF047847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS translocon maturation chaperone LptM"
                     /protein_id="HIR1183083.1"
                     /translation="MLRTAMLMSIIATLCAGLSGCGLKGDLYMPDRPHAEQTTSQQSG
                     R"
     gene            20023..20328
                     /gene="cyaY"
                     /locus_tag="ACZPB6_00975"
     CDS             20023..20328
                     /gene="cyaY"
                     /locus_tag="ACZPB6_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HIR1183084.1"
                     /translation="MDKDTEFLTMAEAEMASLENWLEEAGDDFDCSRSGNVLTIELDS
                     GEQVVVNIQTPMHEIWCASRFGGHHFSWREGAWRDSRTDRTLEDMIAEALRKLGGRI"
     gene            complement(20426..22807)
                     /locus_tag="ACZPB6_00980"
     CDS             complement(20426..22807)
                     /locus_tag="ACZPB6_00980"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR1183085.1"
                     /translation="MTQTKRTTAARKKPARKSPRAGSVLAWLFGIAAAGIASGVLLAV
                     FVFAFIYRQLPPIDTLADYRPRVPLRVWSADGKLIGEFGEERRDFVRLSEVPDHVKHA
                     ILAAEDDGFYEHPGIEITGIIRAALSNVLTGRRGQGGSTITQQVARNFFLSSERTYTR
                     KLYEIAMSFKIEKTLTKDQILEIYMNQIYLGQRAYGFQSAARTYFGRSLDQISIGEAA
                     TLAGLPVAPSAYNPIVNPTRATMRKNYVLGRMRDLGYIDELTYETEKAQPMQPRRVAT
                     QQEIVISNMSEEFVTAKYAAELARMLVYDVFKEETYSRGLNVYTTIDMGAQKTAVDAV
                     RRQLISYDRKYGYRGPESYIDLGPADKHAENIRAAIAKTTASPFMVAAVVTEADPKVI
                     KAALSANEIVTLDAESLKFGRRHLGKVSKQYQDSALKPGAVIRVMRSADGKHWTLAQV
                     PRVEAAFVAGDFNTGAVRALVGGFDFNLNMFNHVTQAWRQPGSSFKPFIYSAALDKGF
                     STTTIINDAPISIDPRLTGNKLWEPKNYEGRFDGPMPLRIGLEKSKNLVSIRVMQAIG
                     PEYAQEFVRKFGFQPDKHPANLPMALGAGSVTPWQMLGGYTVFANGGYRVQPYLIERV
                     TDVDGRTLMRTTNRIAGDESIRAIDDRNAYIMQSLLHGVATRGTAARASRMLKRGDIG
                     GKTGTTNNSHDAWFCGYAGGLVAVSWMGYDNPKPLGARETGGGLALPVWIDFMKAELK
                     GVPEYTRVMPSSVVERDGEVFYRDPVKGAAGAFSDLLMSGRDEASELVRDQIF"
     gene            23136..23627
                     /locus_tag="ACZPB6_00985"
     CDS             23136..23627
                     /locus_tag="ACZPB6_00985"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR1183086.1"
                     /translation="MQESVFSMPVVVGESSIDLLGHVNNREYMRWMEAAATAHAASLG
                     WSFDALRSAGRVWVVRQHWIEYLRSARLGDELVVATWVESVRRLSSLRRYAVLRNGEL
                     LTVGATEWVLVDYERRTPVQIPEEMRASFRLVAPDAEVLRDMGIRRMVRFAPSPGLAA
                     ASK"
     gene            23634..>24010
                     /locus_tag="ACZPB6_00990"
     CDS             23634..>24010
                     /locus_tag="ACZPB6_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1183087.1"
                     /translation="MEYTVKIEDVTFGYGDRLILQNVSMKFGAGKVVAIMGGSGMGKT
                     TLLKLIGGLLTPQGGRVEVTGETLDPSNQKRLYRIRRRMGMLFQFGALFTDLSVFDNV
                     AFPMREQTDLDEETIRDLVLMKL"
BASE COUNT         4749 a         7098 c         7284 g         4879 t
ORIGIN      
        1 gaactgccgg aaggtgttga aatggtcatg cctggcgaca acatccagat gactgtcaag
       61 ctcatctgcc cgatcgccat ggaacagggt ctccgcttcg ccatccgcga aggcggtcac
      121 accgtcggcg ctggcgtcgt tgccgctgtc atcgaataat cgaaattttt cggtaatttg
      181 acttgatcgc tcgagggagc ggggtgtata ctccgctccc tctgttcttt tatctgttct
      241 tttttttaca ggaatacgaa atgcagtctc agcgcattcg catccgcctc aaggcgtatg
      301 actacaagtt gatcgaccag tcggctctcg aaatcgtcga caccgccaag cgcaccggcg
      361 ccgtggtccg tggtccggtc ccccttccca cccgcatcca gcgcttcgac atccttcgct
      421 ccccgcacgt caacaagacg tcccgcgatc agctcgaaat ccgcacccac cagcgtctca
      481 tggacatcgt tgatccgacc gacaagacgg ttgacgctct catgaagctt gacctcccgg
      541 ctggcgtcga cgtcaagatc aaggtccagt aatcaaattc agactgtcag ctgtgcttca
      601 caagaaaagt cgcaaaatga gttgcacact cagagaatca cagctacaat cgcgcctttc
      661 agtccaaacg gttcactact caggaaccag ttggcttttt catgaaaacg gtcccggcca
      721 atcgcagccg gggtggagaa aacaatgagt gataagctcg gcatcgtcgg tcgcaagatc
      781 ggcatgacgc gcattttcac ggaagacggc gtgtccgttc ccgtgactgt gctcgatgtg
      841 tcgaacaacc gtgtcactat ggtgaagacg gttgaaaccg acggctacaa tgcaatccaa
      901 gtcgcgttcg gctccaagaa gcccacgcgc gtgagcaagc cccttgctgg ccattttgct
      961 aaggctggcg ttgaagctgg cagcatgctc aaagagttcc atatcgaagc tgaacaggtt
     1021 gccgaattca agcccggcat ggccctcaag gccgaaatgt tcacggaagg tcagaaggtc
     1081 gacgtgaccg gcacgaccat cggtaagggt ttccagggtg gtatcaagcg tcaccacttc
     1141 tcgtccaacc gcgcgtccca cggtaactcc gtgacgaccc gtgcccctgg ttctatcggt
     1201 aaccgtcagg acccgggccg cgttttcccc ggcaagcgta tggccggtca tctgggcgat
     1261 gtgcagcgca cgacgcagaa cctcatcatt gcgcgcgttg acactgaacg tcagcttctg
     1321 ctggttcgcg gtgctgttcc cggcgccaag ggcggcgaag tgatcgtccg tccggctgtt
     1381 aaggcgtaag gagcgaaaca atggaactca atttcctgaa cgaacagggc aagaccgttg
     1441 atgtcaacga agccgtgttc ggtcgtgaat acaatgaagc tctggtgcac cagatcgtgg
     1501 tcgctttcca ggctaatgcg cgtcttggta cgcgtgctca gctcacccgt gaagctgtgc
     1561 atcactccac caagaagccc tggcgccaga agggcacggg tcgtgcccgc tctggtatga
     1621 cgtcttcccc ggtgtggcgt ggcggcggtc gtgccttccc gaactccccg gacgaaaact
     1681 tcagccagaa ggtcaacaag aagatgtacc gcgctgcgat ggcttccatc tggtcgaagc
     1741 tcgttgccga cgaacgtctt tgcgttgtcg aatccttcgc ggtcgaatcc ccgaagacca
     1801 aggcttttgc cacgcagctc aagacgatgg gtctgcagaa caaggttctt ctcgtcgttg
     1861 gcgctgaaga actcaccgac aacctcatcc tcgcttcccg caacatgaag gatgtgctcg
     1921 ttgtcccgac ccgttacgcc gatccgctct gcctccttta cttcgacaag gtggtggtga
     1981 cgaaggccgc tgtgtccgag attgaggaga tgctggcatg aaccaggaac gtcttttgaa
     2041 ggtgctcgtc gccccggtga tctctgaaaa gagcaccatg gtcgccgaga agctgaacca
     2101 ggcctgcttc gtcgtcgttc ccgacgccaa caaggccgag gtcaaggctg ctgttgaact
     2161 gctcttcaag gtgcaggtca aatccgttca aatcctgaac ctgaagggca agcagaagcg
     2221 ctttggccgc ttcatgggca agcgcaatga cgtccgcaag gcttacgtca cgctcgctca
     2281 gggtcaggaa atcaacttcg cgcaagaggt gaagtaatgg ctctcgtcaa actcaagccg
     2341 acgtccgccg gtcgccgtca tgcggttaag gttgtcaaca aggagctcca caagggcaag
     2401 ccctgggccg ctctgactga aaagaagaac cgcggttccg gccgtaacaa caacggtcat
     2461 atcacctgcc gtcacaaggg cggcggtgcc aagcgcgctt atcgtatcat cgacttcaag
     2521 cgccagaagg acggtgtgcc cgcccgcgtt gaacgtatcg aatacgatcc gaatcgttcc
     2581 gctcacatcg ctctggtctg ctacgccgac ggcgaacgct gctacatcat tgctccgaag
     2641 ggcctgaccg ttggtcagga aatcatgaac ggtccggaat ccccgatcaa ggtcggcaac
     2701 acgcttccgc tccgcaacat tcccgtcggt tccacgattc actgcatcga aatgctcccg
     2761 ggcaagggtg ctcagatggt ccgcgctgcc ggtgctttcg cccagctcgt ggcccgtgaa
     2821 ggtgaatacg cccagatccg cctccgttcc ggtgaagtcc gccgcgttct catcgaatgc
     2881 cgcgccacga tcggtacggt cggcaacgaa gaaaacagcc tccgcgaact cggcaaggcc
     2941 ggtgccaagc gctggcgcgg tgtccgtccg acggtccgtg gcgttgccat gaacccggtt
     3001 gaccatccgc atggtggtgg tgaaggcaag accggtaccg gtcgcgctcc tgtcacgccg
     3061 tggggtcagc tcacgaaggg ctaccgtacc cgcaactgca agcgcacgga caacatgatt
     3121 gtctcgcgtc gtaaccgtaa ataagggggc ctgaatgtct cgttccttga agaaaggccc
     3181 gtttgttgac gggcacctga tgaagaaggt tgaagccgcc caggcgagcc gcgacaagcg
     3241 tccgctcaag acctggtccc gtcgctccac gattcttccc gagttcatcg gtctgaccat
     3301 cgccgtccac aacggccgtc agcacgttcc ggtgtacatc aatgaaaaca tggtcggcca
     3361 caagctcggc gaatttgcga atacccgcac gttcaagggc cacgctgccg gcgacaagaa
     3421 ggtgggtaag taaatggaaa ctactgctat cgtccgtggt gtccgccttt cggctcagaa
     3481 gggtcgtttg gttgctgacc tcgttcgcgg caagcccgtg gacaaggctc tcgccatcct
     3541 caccttcacg cagaagaagg ctgctgtcat catcaagaag gctcttgaga gcgcaatcgc
     3601 caatgccgag cacaatgacg gtgccgacat cgacacgctg gtcgtgacca agattcacgt
     3661 ggaaaaggct gctacgctgc gccgtttcgc tgcccgcgct aagggccgcg gcacccgcat
     3721 caacaagcag accagccata tctatatttc tgttggcgac gcgaaagcta agtaaaggtg
     3781 taccatgggt cagaaaatta atcccaccgg cttccgtctt cccgtgacgc gcaattggac
     3841 gtcgcgttgg tacgcggttg gccgtgattt ttcccgcacg ctcggtgaag acctcgctgt
     3901 tcgcagcttc cttctcaaga agcttcgcaa cgccagcgtc agccgtattc tcatcgaacg
     3961 tccggctaac aatgctcgca tcacgatctt ctccgcacgt ccgggtatcg tgatcggcaa
     4021 gcagggtgcc gacatcgagg ctctcaaggc tgaagttcag aagatgatgg gcgtgcccgt
     4081 tcacgtcaac atcgaagaag tccgccgtcc ggaactcgac gctcagctca tcgctgacgg
     4141 catcacgcag cagctcgaaa agcgcgttat gttccgtcgc gccatgaagc gcgccatgca
     4201 gaacgccatg cgtctcggtg cccagggcat caagatcatg tcgtccggcc gcctcaacgg
     4261 tgccgacatc gcccgttgcg aatggtaccg tgaaggccgc gtgcctcttc agacccttcg
     4321 cgccaacatc ggctacggct tctccgaagc ccttaccacg tacggtatcg tcggtgttaa
     4381 ggtctgggtt tacaagggtg acaacttcgg ccaggaagaa gctgttgaag ctccccgcga
     4441 agatcgtcgt ggccgtcgcc ccggtgaccg tgccggcaag ccgggtgctc gccgcaacaa
     4501 cggtcgtcgc aatgacaagc agcagcaggc ccgcaagccg gttgctaagg acggagaata
     4561 acgatgctgc aacctaatcg tcgcaaatat cgtaaggacc agaagggccg taactatggt
     4621 ctcgccacgc gcggtgccaa tgtttctttc ggcgaattcg gtctgaagac gctcgaacgc
     4681 ggccgcatta cggctcgcca gattgaagct gcccgtcgcg ccatcacccg ccacattaag
     4741 cgtggtggtc gcgtgtggat ccgtgtgttc ccggacaagc ccatctccag caagcctgct
     4801 gaagtccgaa tgggtaacgg taagggtaat ccggagtact gggttgcgct ggttcagccg
     4861 ggccgtgtgc tctatgaaat ggacggggtt gacgaacagc tcgcccgcga agcttttgct
     4921 ctcgctgccg ccaagctgcc cgtgaagacc acgtttgttg tccgccagat cggcatgtaa
     4981 ggagacggac atgaacgcta aggaactgcg cgccaaggat gaggccgcgc tcaaacagga
     5041 actgaacgac cttcagaaca cgcacttcaa gctgcgtatg cagaaggcca cccagcagct
     5101 cacgaacacg agttcgctgc gcacgacgcg tcgtgatatc gcgcgcgttc gcacgatcct
     5161 taccgaaaag gcgaccacga aatgacggaa accgctcagt ctctgaaccg taccctcgtc
     5221 ggcacggtct ccagcagcaa gatggaaaag accgttacgg tcctcgttga acgtaaggtt
     5281 aagcatccgc tgtatggcaa gtacgtcgtc aagagcaaga agtatcatgc tcacgacgaa
     5341 attggctgca acgaaggcga caaggttgaa atcgctgaat gccgtcccct ctccaagacc
     5401 gtgtcttgga cggtgacgaa ggttctcgaa aaggccgttg tcctctaagt tgaagccggc
     5461 gaaaaaaaat cgccagattc gcttgcacag ctctaaacat tagtttatag ttgcaagcca
     5521 atcgcccggg gtacccctag cggtgtctcg ggcgattact tttcttgggt tgtgaattgt
     5581 ccgcctcccc ttgcgccagg caaggcttcc gagacggccg atttactgcc ttcctcccct
     5641 aaacgggacc aatactatcc gctaatgtgg aataagttgg gattttctaa accatgattc
     5701 agatgcaaag ccgactggac gtcgccgata acaccggtgc tcgttcggtg atgtgtatca
     5761 aggtgttggg cggctccaag cgccgttatg ccaacatcgg tgacgtcatc aaggtgactg
     5821 tcaaagaagc ggccccccgc ggccgtgtca agaagggcga agtctacaat gcggtcgtcg
     5881 ttcgcaccgc tcacggcgtt cgccgcccgg atggctcctc catcaatttt gatggcaatg
     5941 ccgccgttct tctcaacagc aagctggagc cgattggcac ccgcatcttc gggccggtca
     6001 cgcgcgaact gcgtaccgag cagttcatga agatcgtgtc gctcgctccc gaagttcttt
     6061 aattaaggag aagcgatgca gcgtatccgt aagggcgacg aagtcgtcgt cattgctggc
     6121 cgtgacaagg gcacgaaggg caccgtcctc tcccgcgttg acgacagcca tgtcatcgtc
     6181 gaaggcgtga acgtcgttaa gaagcacgtt cgtccgaatc cgcatctcgg cgttgccggc
     6241 ggcattgttg aaaagaacat gccgatcgat cagtccaacg tcatgctggt caacccggaa
     6301 acgggcaagg gcgaccgcgt cggcttcaag gaagttgacg gcaagaaggt tcgcgtgttc
     6361 aagagcaacg gtgccgttgt cggcgccaag gcctaatgag ggtgacaatg tctcgtttta
     6421 gttccatgta tcacgacacc attgtccccg aactcatgaa gaagttcggc tacaagacca
     6481 cgatgcaggt tccgcgtatc actaagatca cgctgaacat gggtgtcggt gaagccgtta
     6541 acgacaagaa gcttgtcgac gccgccgttg gcgacatgac caagatcgcc ggccagaagc
     6601 cgatggtcac ccgcacccgc aaggctatcg cgaacttcaa gatccgcgaa aacatgccga
     6661 tcggctgcat ggtcaccctc cgtggcgaac gcatgtatga cttccttgac cgtctcgtga
     6721 ccgtcgcttt cccgcgcgtc cgtgacttcc gtggtgtctc cggtcgtgct ttcgacggcc
     6781 gcggcaacta taacatcggt ctcaaggaac agatcatctt cccggaaatc gaatacgaca
     6841 agatcgatcg tctccgtggc atgaacattt ctattaccac gacggcgaag tccgacgaag
     6901 aagccaaggc tcttctcgcc ggtttcagct tcccgttccg caactgaggt tgacgcaaaa
     6961 tggcaaaact tgctctgatt aaccgtgaag ccaagcgtgc tgctacggtt gcgaagtatg
     7021 cggccaagcg cgccgccctc aaggcaatta ttaacgacgc tacccgctcg atggaagagc
     7081 gcctggaagc ccgcgccaag ctgcaggctc tcccgcgcga cgcgagcccg gtccgccagc
     7141 gcaatcgttg cgccctgacc ggtcgtcccc gtggcacgtt ccgcaagttc ggtctgtgcc
     7201 gtggcatgat tcgtgatgcc gccatgcgcg gtgacattcc tggccttgtc aaggctagct
     7261 ggtaagcgag gagattacga caatgagtat gagtgatccg atcgccgaca tgctgacccg
     7321 catccgcaat ggtcagatcg tcgaaaaggc cgaagttgtg atgccgtcct ccaagctgaa
     7381 ggtcgccatc gccaaggtcc tcaaggatga aggttacatc gacggctaca ccgtcgttgc
     7441 caacgaaggc aagcccgaac ttcacgttgg tcttaagtac tacgccggtc gcccggtcat
     7501 cgaacgcctc gaacgcgttt cccgtcccgg cctccgtatc tacaagaacc acggttccat
     7561 tccccaggtt atgaacggtc tcggtatcgc gatcgtttcc acgccgaagg gtgtcatgac
     7621 cgaccgcgct gctcgttccg ccggtatcgg tggtgaagtt ctttgctacg tcgcctaacc
     7681 gaggagtgtg aaatgagtcg aattgctaag attccggttc agatcgttca gggcgtcagc
     7741 tacacgctga ccgctgacaa catcacggtt aagggcccgc agggtgaagt ctccatgcac
     7801 attcttccct tcgtgaccat cacggaagaa gacggtgctc tgcacttcaa gcaggttgaa
     7861 gaaacccgcg aagccaacgc caacgttggc acgatgcgcg ctctcgtggc tgacatggtc
     7921 aagggttgtt ccgttggctt ccagaagaag cttcagctcg tcggcgtggg ctatcgtgcc
     7981 caggctcagg gcgacaagct caatctctcc ctcggcttct cccacccggt cgtccatcag
     8041 atgcccgctg gtgtcaagtg cgaaacgccg agccagactg aaattttgat caagggcgct
     8101 gacaagcaga aggtcggtca gaccgctgcc gtcattcgtt cgtaccgcgc tcccgaaccc
     8161 tacaagggca agggtgtccg ctacgccgac gaagtcgttg tgatcaagga aaccaagaag
     8221 aagtaatcgg ggcgatacaa aatgatcaac aagaaacaaa gccgcttgcg tcgtgctcgt
     8281 gccacgcgcg ctcgcattct gttgcagaag gtcgatcgcc tgacggttca tcgcaccaac
     8341 ctgcacatct acgccagcat catctcggcc gacaacggcc gtacgctggt ttcggcctcc
     8401 acggtcgaac ccgaagtgcg tgctcagctc gcgaacgtct ctggccgcgg tggcaacgtt
     8461 gccgctgcca agctcgttgg cacgcgtctt gccgaaaagg ccaaggctgc cggtattgaa
     8521 tcctgcgcct tcgaccgttc cggctaccgt taccatggcc gtgtcgccgc gctcgctgaa
     8581 gctgcgcgcg aagccggtct caagttctaa ggcagggcga aatggctaag gctcaaggta
     8641 aaaatcagat ggtcgacgaa cagggcgacg gtctgtgcga aaagatgatc gcggttaacc
     8701 gcgtcacgaa ggttgttaag ggtggccgca ttctcgcttt cgccgctctt acggttgttg
     8761 gcgactgcga cggtcgcgtc ggcatgggta cgggcaagtc ccgcgaagtg ccgcagtccg
     8821 tcagcaaggc catggatcgt gcccgtcacg acatgaagcg cttcccgctc aagaacggca
     8881 ccattcatca cgctgtcgaa ggccgtcacg gcgcttcccg cgtgatcatg atgcccgccc
     8941 cggaaggtac cggcgtgatc gccggcggtc cgatgcgcgc tgtttttgaa gctgtcggca
     9001 tccgcaacgt cgtcgccaag gcctacggtt ccacgaaccc gtacaacctc gttcgtgcca
     9061 ccatccaggc tctcaacaat ctgcgtagcc cggccgaaat tgctgctaag cgcggcaaga
     9121 cggtcgaaga gctgctcggc taatcaggag tccaagacat gtccgaaaag aagaccgtta
     9181 aggttactct tgtcaagagc cccatcggta cgaaggctga tcatcgcgct acgctcgctg
     9241 gcctcggtct caagaagatc cgccagtctc gcgttctcga agacacgcct gctgtccgtg
     9301 gcatgatcaa caaggttgcg tacctcgtgc gcgccgaatg atcgaaagga taacgaaatg
     9361 cgtttgaata cgatcaagcc cgctgagggc tctaagcacg cccaccaccg cgtcggtcgc
     9421 ggtgttggca gcggttgggg caagactgcc ggccgcggcc acaagggcca gaagtcccgt
     9481 gccggtggct tccacaaggt cggtttcgaa ggcggccaga tgccgatgta tcgccgcctc
     9541 ccgaagcgtg gctttacctc gctcacccgc aagtactgcg aggtggtgcg tctgtccgat
     9601 ttggaacgtc tgagctgtga tgaaatcgat ctggccgtcc tcaaggctgc cggcgtcgtt
     9661 tccgcccttg ctcagaacgc ccgtgtgatc ctcgccggtg aaatcacccg caaggtcacc
     9721 gtgcgtggtc tcggcgtcac caagggtgcc cgcgctgcta tcgaagctgc cggcggctcc
     9781 attgctgaca acgaataaga ctgctaacca aagaggacac cgacgtggcg accagcccca
     9841 gctctaagca acagtcgggc ctcagcaagt acggcgacct taagggtcgc ctgatcttcc
     9901 tagcgctcgc gctcgttgtt taccgcatcg gtgcacatgt gccggtgcca ggtatcgatc
     9961 ccgatatgct caagcagctc ttcgatgagc agcagggcgg cattctcggg ctgttcaaca
    10021 tgttctccgg tggcgccttg tcgcgcttct cggtgttcgc gctcggcatc atgccgtaca
    10081 tctctgcatc gattatcatg cagatgctgt cgtacgtgct gcctgagctt gaagcattgc
    10141 gaaaggaagg cgagtccggc cgacgcaaga tcacccagta cacgcgctac ggcacgttgc
    10201 ttctgggtct cttccaaggt ttcggcattg ccgtcgctct cgaaagccag gctggtctgg
    10261 tgcttgatcc gggcttcatg ttccggatca ccaccacgat ctccttgctg acgggtacga
    10321 tgttcctgat gtggctcggt gaacagatca ccgaacgcgg tcttgggaac ggtatttcga
    10381 tcatcatctt cgccggtatt gttgctggcc tcccgagcgg tacgtctggt ctcttccagc
    10441 tcgtgtccac gggtgccatc agcccgctgg cggccatctt cgtgattgcg atcatcctgg
    10501 cggtcaccgc cttcgtggtc ttcatcgaac gtggtcagcg tcgaatcacc gtcaactatg
    10561 ccaagcgcca gatcggcaac aagatctacg gcggccagag ctcgtatctg ccgctcaaga
    10621 tgaacatgtc gggtgttatt cccccgatct ttgcttcgtc tctgatcctg ttcccggcta
    10681 cgctggccgg ctggttcacc tccggcgact ccactcgctg gatccgtgat cttgccgcag
    10741 ccctttcccc gggccagccg ctgtacacgc ttctgtacgc agtgctgatt gttttcttcg
    10801 cctatttcta cacggctctt gttttcaatc cgaaggaaac ggcggaaaac ctcaagaaga
    10861 gtggtgcgtt cattccgggc atccgtccgg gtgatcagac cgcccgctac atcgaccgag
    10921 tgcttctgcg cttgaccttg ggcggtgctg tctatctggt cttcgtgtgc cttgtgcctg
    10981 aattcctgaa ccttcgcttc aacgtcccgt tctacttcgg cggcacctcg ctgctgatcg
    11041 tggtcgttgt cacgatggac ttcatgagtc aggtccaggc ttacctgatg acccaccagt
    11101 atgaaagttt gctgcggaaa accaacttcc gcggtttggg tcagcccaag cactgatcag
    11161 tcagtgcggg ttgacttaca atcaaccgat gctcggcccc ggtcttaacc accggggcaa
    11221 gcatggaatg atgaccgagg gcgcggccgg agacggcctg cgcccccgat tttcattaaa
    11281 ttattgcggt tggacaacaa agagtgcact acgctcgttt ttgacttgca ataaacagaa
    11341 agctgtagta ttcttctgcg ctttcctgcc atgtgggcat tgttatccgt gcttacgcac
    11401 gtggagatga gaatgaaggt taacgcttcg gtcaaaaaga tgtgccgcaa gtgcaagatc
    11461 attaagcgca aaggcgtggt gcgcgtgatc tgtgctgacc cgcgtcacaa gcagcgtcaa
    11521 ggctaaagag gtaaaaaatg gctcgtatcg ccggtattaa cattccgccg cagcagcatg
    11581 ccgaaatcgg tctcactgcc atctatggca tcggccgttc tcgcgctcgt gaaattctga
    11641 cgaattgcgg catcgaattt tccaagaagg tcaaggatct gaccgacgct gatctcgaaa
    11701 agatccgcga acaggttggc aagtttacgg ttgaaggcga cctccgtcgc gaaattcagc
    11761 tctcgatcaa gcgtttgatc gaccttggca cctatcgtgg tatgcgtcac cgcaagggtc
    11821 ttccctgccg cggtcagcgt actcgtacga atgctcgcac ccgtaaaggc ccgcgtaagg
    11881 ccggtgtgac gctcaagaag taataaagga caactatggc tggcaataat tccaagcagg
    11941 ctgctgcgca gcgtgctcgt aagaaggtca agaaggttgt gacggaaggt atcgctcacg
    12001 tgcatgcttc cttcaacaac acgatcatca cgatcactga ccgccagggc aacgccattt
    12061 cggcgtcttc ctccggcgct cagggcttca agggttcccg taagtccacc ccgttcgctg
    12121 ctcaggttgc tgcggacgtc gctggcaagg ctgctcttga atacggtctt aagaacgtcg
    12181 aagtccgcat ttggggcccc ggtcccggcc gcgaatcctc gattcgcgct ctcaatgcgc
    12241 tcggcattcg tgtgacgtct attcaggatg tgacgccgat cccgcataac ggtgtgcgtc
    12301 cctccaagcg tcgccgcgtc taatttttca tttttctgtt gaatcccgtg tccacatgca
    12361 tactgaggca cgtggactga atgtagggat acctacagga taagagaggc aattttaaat
    12421 ggcacgatat ctcggtccta agctcaagct ttcgcgtcgc gaaggcattg atcttaattt
    12481 gaagagcgcc cgtcgttcgt ttgagtccaa ggtcaaggac gccggttcca agccgggcca
    12541 gcacggtaag atttccggag cccgcacgtc cgactacggc gctcagatgc gcgaaacgca
    12601 gaaggccaag cgtctttatg gcgttctcga acgtcagttc cgtcgttact tcgcggaagc
    12661 tgaacgtatc accggcaaca ccggtgaaaa ccttctcgcc cttcttgaat cccgtctgga
    12721 caacgtggtt taccgcatgg gcttcggctc cacgcgcgct gaagcccgcc aggtggtttc
    12781 tcacggcgcc atccttgtca acggcaagaa ggtgaacatt ccttcttacc acgtcaagcc
    12841 gggcgacgtc atctccgtgc gcgaaaaggc ccagaagcag gctcgtatcg ctgaagccct
    12901 tgaactggct cagcagaacg gcttcccggg ctgggttagc gtcgaaccga agaagttcga
    12961 aggcacgttc aagaacgttc ctgctcgcga tgaaatcgct tacgaaatca acgaagctct
    13021 catcgtcgaa cgctactccc gctaatatcg cttgaatgta cttgtggagc tcggtgtagt
    13081 gcgctgtagc tccgcagaca caaggcagga aaagcaatgg aaaacgcggt cacgcaagtg
    13141 tccgcgtttt cttgcctgat gggcccgcgt ttcggtccct catttgaaag cgtcctttac
    13201 tccagagggc gctttcaaat ggtctacaat ggccgtgcgg ccgggcagcc gccgcaattc
    13261 tgtgctgccg ccttattcgt ccgcgggctt aacgcgcccg ggggaatgca aaggatctca
    13321 aactatggct aacacctcgc ttctgaagcc gacctctgtc gaagtcgcgc tttccgaaaa
    13381 caatccgaac cgcgccgtca tcacgctcga accgttcgaa cgcggctacg gccacacgct
    13441 cggcaatgcg ctccgccgca tccttctgag ctccatgatc ggctttgcgc cgacggaagt
    13501 ccagatcacg ggcatcgttc acgaatactc ccagatcgac ggcgttctcg aagacgtcgt
    13561 tgacatcctt ctcaacctca agggtgttgt cttcaagctt gacggccgcg acgaagtcac
    13621 cgtcatgctc cgcaaggacg gcgaaggcgt tgtgaccgcc gccgacttcg atcttcccca
    13681 cgacgtttcc gtcgtcaatc ccgaccacgt gatcgcccac ctctccggcg gtcgtctcga
    13741 aatgcaggtc aagattgaaa agggccgcgg ctaccagccg ggtgacgtcc gcgccttccg
    13801 cgatgactac tccaaggctg ccatcggccg catcatcatg gatgcctcct tctccccgat
    13861 cctccgcgtt gcctatcagg ttcaggccgc ccgcgtggcc cagcgcaccg acctcgacaa
    13921 gctcgagatg acgatcgaga ccaacggcgc catcggtccc gaagacgccc ttcgtgaagc
    13981 cgtccgcatc ctccaggatc agctcgccgt gttcggcatg atccatcagg acaccggcgc
    14041 cgacgctgct cctgccgagg aagacgaaga cgttccgccg ccgctcgatc cgatcctgct
    14101 ccgtccggtc gacgatctcg agctcacggt tcgttccgcc aactgcctca aggccgagaa
    14161 catctactac atcggcgatc tgatccagcg cacggaaaac gagcttctca agacgccgaa
    14221 cctcggccgc aagtccctga acgaaatcaa ggaagagctc gccgcccacg gcctcacgct
    14281 cggctcccgt ctcgaaaact ggccgcccgc ccaggaacac taatggacag gaagggcgtc
    14341 gtcgcagtcc gatgaaggct caaaatttca tctgatttcg tttgacgccc gattgttgct
    14401 gtgcaataat cccgtcctgc cgtttggctc acatgggcgt gatctcgcga tcacgcccat
    14461 cgctttcacc cgaccgctcg aatttctcaa gagatagaag actcggggca agcctgccgg
    14521 tcaaagccgg catcctttca tagatatttt taggaacata caaaatgcgt caccgtcttg
    14581 gtctccgcaa gctgaaccgt accagcgccc accgtctcgc catgcttcgc aacatgatgt
    14641 gctctctcat caagcacgaa gccatcaaga cgacccttcc caaggctaag gaacttcgtc
    14701 gcgtcgtcga accgatgatc accctcgcca aggaaccgac cgttgccaac aagcgcctcg
    14761 ccttcaaccg tctccgtgac cgcgaagtcg tctgcaagct gttcaacgaa atcggcccgc
    14821 gctttgccaa ccgcaacggc ggctacaccc gcatcctcaa gatgggcttc cgcgttggcg
    14881 acaacgcccc gatggcctac atggaactca cggaaaaggc tgttgccgtt gaagaagtca
    14941 agtccgaaga ataatttctg aggctgcact gctttgacaa aggctcgatc gttgctgcgg
    15001 tcgggccttt tttctcatta gggagactga tttggacgct atgcgcatcg acaagtggct
    15061 ctgggccgcc aggttcttca agacccgcac catcgcacag gaggaggtcg gccttgggcg
    15121 cgttctcatc gacggtcagc gcatgaaggc atcccgcgac gtgcgcgtcg gagaccgcct
    15181 cacgatccgc cgcggcgagg agaccttcca tgtctttgtc gaaggactct ccaatactcg
    15241 cgggcctgcc gctgaggctc agaagctcta tcgtgaaacc gaggagagca agatgacccg
    15301 tgcgcagatg aaggagatgc tgaggcttgc ctccgagccc gcctcctaca ttcgtcaggg
    15361 acgaccgacc aagaaggacg gccgcctgct gagggaatgg cagcacaaaa tgaagtaagg
    15421 gcctttccgg ctcccaaagc gacgaaagcc cgcacatcgt gcgggctttc gtcattcagg
    15481 aatcctgacg tgaggctcag tagttgtagc cttcgctgat ccagcgggcg acgtcaaggg
    15541 cgcagtacgt caggatgccg tcggcacccg cgcgcttgaa gcagaccatc gtctcgaagg
    15601 tgatcttctt ttcgtcgatg gcgccggcct gagcggcgaa cttgatcatc gcgtattcgc
    15661 cggagacgtg gtaggcgaag gtcggagcct tgaactcctg cttgacgcgc cagagcacgt
    15721 cgagataggg aacgcccggc ttgaccatga ccatgtcggc gccttcggcg aggtcgaggc
    15781 ccacttccca gagggcttcg ttgccgttgg cggggtcctg ctggtagacg gccttgttgg
    15841 acttgccgag gttggaggag gaacccacgg cgtcgcggaa cgggccgtag taggagctgg
    15901 cgtacttggc ggagtaggcc attatgcgcg tgtggatgag accttcatcc tcgaggcgct
    15961 tgcggatgat gccgatgcgg ccgtccatca tgtcggaggg cgcaacgacg tcggcgccgg
    16021 ctcgggcctg agcgagtacc tgctggacga gcatttcgat cgtctcgtcg ttcaggatgt
    16081 agccttcttc gtcgatgagg ccgtcctgac cgtgcgtggt gtacgggtca agcgcaacgt
    16141 cggtcatcac gccgagctgc ggataggcgg ccttgagggc cttgacggcg cgcgggatca
    16201 ggccgtcggg gttcgcggct tcacagccgt cgggcgtctt cagggcttct tcgatgtgcg
    16261 ggaagagcgc gatcatcgga atgccgagtt ccacgcactc gccggcgacc ttgaggagtt
    16321 cgtcgatgct caggcggtcg acgccgggca tggacggaac cggctcgcga aggccggtgc
    16381 cctcgatcac gaagaccggg agaatcagat cgttgggggt cacgacgttt tcgcgcatca
    16441 ggcggcggga gaagtcgtca tgacgcatgc ggcgcatgcg aacgctggga taagcgccaa
    16501 gagtctgcat ttggtgttcc tacgaataaa aagacggctt cggaggtgcc gaagcctttc
    16561 tgaagcattg cccttacttt attccttcgg gctttcctga gcattaaggc caagccagcc
    16621 ctcaagcacg gcgccgagtt cctcggtgcc ttccttcttc atgcccgaga agagctggac
    16681 cgtgacgtgg gggccgatct cggcggcgcg cttctgcgcg ttgcgaagcg tcgtgcgcct
    16741 ctcctgattc ttgagctggt cggccttgtt gagcagccag tgcatgcgca cgttggggcg
    16801 gcctgccatg aattcgatga gccactcgtc ggtgggcatg aagggacggc gggcgtccat
    16861 cacgatgacg agaccgacca tgttcgggcg ctgcgcgatg tagccgccca cgagttgcga
    16921 ccaggtcttg cgggtgtctt cgctcgcctt ggcaaagccg tagcccggaa ggtcgacgag
    16981 gcagccgacg tcctcgcgca cgcccgcctc gttcttcttc gacagggcga agaaattgat
    17041 gagttgggtg cggcccggcg tcttgctcgc gaaggcgagg cgggtcttgc gcgtcagcac
    17101 gttgatggtg gtcgacttac cggcgttgga gcggccggca aaggcgattt caggcacggc
    17161 ttcgggcggc aggcccgaga gcttggcggc ggagatgaca aaacgagcgg tgtcgaagat
    17221 gctcaaggta acctcgtcgg tgaaaagtct tggagtgccg tcattgtact gcgcagggca
    17281 cgaaaagacc cccgaatctg gcaagaatcg ggggcctgcg tcaggaggga cggcgcggat
    17341 tacggcgcct tgccgaagac ctcgttgagc tgcatggaca cccggacgaa ggtcgtgcgc
    17401 atggtgagct ccttgaggcg gcgcgcgccc acgtaggtgc aggcgctcct cacaccgccg
    17461 aggatgtcct ggatggtgct ttcgacggga ccgcgggcgg gaatgaggac ggccttgccc
    17521 tcggccgcgc ggtacttggc cacgccgccc gcgtacttgt ccatggcgtt gcgagagctc
    17581 atgccgtaga agcgcttgaa gaccccgtct tcagtcgtga cgacgtcgcc tgcggcctcg
    17641 tcatggcccg caagcatgcc gccgagcatc acgaagtcgg caccgccgcc gaaggccttg
    17701 gcgatgtcgc ccggcaccgt gcagccgccg tcggcgcaga tgcggccgcc gagaccgtga
    17761 gccgcatcgg cgcactcgat gatcgcggag agctgaggat agcccacgcc ggtcatcttg
    17821 cgggtcgtgc agacggagcc cgggccgatg cccaccttga cgatgtcggc gccagcaaga
    17881 agaagctctt cagtcatgtc gcccgtgacg acgttgcccg ccatcagcac gatgtcggga
    17941 taggtttcgc gaaccttctt cacgaaggcg acgaacgcct ccgtgtagcc gttggcgacg
    18001 tcgatgcaga catagcggat cgcgccggga gcggcagcca tcacctcgcg gaacttctgc
    18061 cagtcgtgct cgccgatgcc gagcgaatag aaggcgtcgg acttgttctc aagcttctgg
    18121 aaaaagtcga catattcctt ggccgtgtag tgcttgtgaa gagcggtgga cattttgtgc
    18181 ctgccgaggg cggacgccat ctcgaaggtg ccggtcgtgt ccatgttggc ggcgatgatg
    18241 gggatgccgt ggtagggctc ggaagagtgc aggaacttga attcacgggt gatgtccact
    18301 tcgctgcggc tcgtgagcgt cgagcgcttc gggcggatga ggacgtcctt gaagtcgagt
    18361 ttgacggaat tctcaagatg catggctgac tccttctgtc tgggcgttcg ggcatggccc
    18421 tgtctgcaga cacaaaaaaa cgccgcatct cgggagtgtc tccgagatcc ggcgtttacc
    18481 cattttacgg ggttgctttg aaaaaccgca agtcagtcga aagtagtagc cccgtcaggc
    18541 gtctcaaagt tcgagcagaa cttcgccgct caccatttcg tcgaacgtgt cgggagtgcg
    18601 cagcgggagg acgcgctctc cgtcgatcat gacctccatg ggaagcatgc ggctgttgta
    18661 gcggctggcc atgcttgcgc cgtaggcgcc cgcatcgcac acggcgatcc agtcaccctc
    18721 cctgacggcg agctcgcggg cgcggccgag gaagtcggac gattcgcaca cggggccgac
    18781 gacgtccatg acaagcggcg cggcgtcatg tcgttcgacg acggggacga tgtccatcca
    18841 ggcggagtag agggccggac ggatgaggtc ggtcatggca ccgtcgacga tgcagaagtt
    18901 tcgcgtctcg ctctgcttga tgtattcgac ctgagtgagc atgagacctg catcgcccac
    18961 gatcgagcgg ccgggctcga ccatgaggac cgcgtcgccg aagccgcgct cctccatcat
    19021 gcggcgcacg gggcgcagca gggtctgcgg cgtgggaggc tggtcctcgg gacggtactg
    19081 gatgccgagg ccgccgccca tgtcgaggtg atggagcacg agacccttgt cgcggagtgc
    19141 ggacatcgtg tcaagcagtc ggtgcacgga gtcgaggtag ggctggattt cggtgatctg
    19201 gctgccgatg tgcgcatcga tgcccttgac ttcaatgccg ggcatgttgg cggcgcgcag
    19261 gtagatgtcc tccacgtccg cgatggcgat gccgaacttg ttgttcttga ggcccgtgga
    19321 aatgtagggg tgcgtcttcg gatcaacgtc gggattgcag cggatcgaga tgggtgcgcg
    19381 cacgccgagc tcggtcgcaa tgcggttgat ccgctcgagt tcgggctcgc tctcgacgtt
    19441 gaagcagccg atgcccgcct tgagcgcgaa ggtgatctcg cgggcggtct tgccgacgcc
    19501 ggagtagacg atcttctcgg ggcggaagcc ggcgcgaagc gcgcgggcga tttcgccgcc
    19561 gctcacggtg tcgacgccgg cgccgaggcg cgcgagcacc tgaagaacgc cggccgtcga
    19621 gttggccttc atggcatagc agacggcatg cgggacgtcg gcgagcgcct cttcgtaggc
    19681 gaggtagttg cgctcgatgc gggcgcggga atagacgtag gtggtctgct cgagtcggcg
    19741 ggagagttcg agcagggaga cgccttcgca gaagaggacg ccgtcctccc tgtgaaagca
    19801 gggcctgtgg ttgttttcag acatcttgag ggaaaagtgc aggggcggtt cgtatgctaa
    19861 agtccggaaa tcaacggccg gactgctggc tcgtcgtctg ttcggcgtgc gggcggtcgg
    19921 gcatgtagag gtcacccttc aggccgcagc ccgagaggcc tgcgcacagg gtggcgatga
    19981 tgctcatgag catggcggtt ctcaacatag gaagtccttg caatggataa agatactgaa
    20041 tttttgacaa tggccgaagc cgaaatggcg tcccttgaaa actggctcga agaggccggg
    20101 gatgatttcg actgcagccg cagcggcaac gtgctgacga ttgagctcga cagcggcgag
    20161 caggtcgtcg tcaacattca gacgccgatg catgaaatct ggtgcgcctc gcgcttcggc
    20221 ggccaccact tctcatggcg tgaaggcgca tggcgcgaca gccgcacgga ccgcacgctc
    20281 gaggacatga tcgccgaggc gctccgcaag ctgggcggcc gcatctgatc tgaagagtgt
    20341 agcggacatt tccgtactcc atataaggaa aagagaccag aagacatgag ttcttcggtc
    20401 tcttttcgtt ttttcagggc tgtcgtcaga agatctggtc gcgcacgagc tcgctcgcct
    20461 cgtccctgcc tgacatcagg aggtccgaga aggcgcctgc ggcacccttg acgggatccc
    20521 tgtagaagac ctcgccgtcg cgctcgacga cgctcgaggg catgacgcgc gtgtattcgg
    20581 gcacgccctt gagctcggcc ttcatgaagt cgatccagac gggcagcgcc agaccgccgc
    20641 ccgtttcgcg ggcgccgagc ggcttgggat tgtcgtagcc catccaggag acggcgacga
    20701 ggccccccgc atagccgcag aaccaggcgt catgactgtt gttcgtcgtg ccggtcttgc
    20761 cgccgatgtc gccgcgcttg agcatgcggc tcgcgcgtgc ggcggtgcct cgggtggcga
    20821 cgccgtgcag caggctctgc atgatgtagg cgttcctgtc gtcgatggcg cggatggatt
    20881 cgtcgccggc gatgcggttc gtcgtgcgca tgagcgtgcg gccgtcaacg tcggtgacgc
    20941 gttcgatcaa atagggctga actcggtagc cgccgttggc gaagaccgtg tagccgccga
    21001 gcatttgcca gggcgtgacg ctgcccgcgc cgagtgccat cggcagattg gcggggtgct
    21061 tgtcgggctg gaagccgaac ttgcggacga actcctgcgc gtactcgggg ccgatggcct
    21121 gcatcacgcg gatcgagacg aggttcttcg acttctcaag acctatgcgg agcggcatcg
    21181 gaccgtcgaa gcggccttca tagttcttcg gctcccagag cttgttgccc gtgagcctcg
    21241 gatcgatgct gatcggggcg tcgttgatga tcgtggtcgt cgagaagccc ttgtcgagtg
    21301 cggccgagta gatgaagggc ttgaaggacg aacccggctg acgccaggcc tgcgtcacgt
    21361 ggttgaacat gttgaggttg aagtcgaagc cgcccacgag cgcgcgcacg gcacccgtgt
    21421 tgaagtcgcc ggcaacgaac gcggcctcga cgcgcggcac ctgggcgagc gtccagtgct
    21481 tgccgtcggc ggagcgcatc acgcggatga cggcgccggg cttgagcgcg ctgtcctgat
    21541 actgcttgga gacctttccg agatgacggc ggccgaactt caggctttcg gcgtcgagcg
    21601 tcacgatctc gttggccgac agggcggcct tgatgacctt gggatcggct tcggtgacga
    21661 cggcggcgac catgaaggga gaggcggtcg tcttggcgat cgcggcgcgg atgttctcgg
    21721 cgtgcttgtc ggcggggccg aggtcgatgt aggactcggg accgcggtag ccgtacttgc
    21781 ggtcatacga gatgagctgg cgtcggacgg cgtcgacggc cgtcttctgg gcgcccatgt
    21841 cgatcgtcgt gtagacgttg aggccgcgcg agtaggtctc ctccttgaac acgtcgtaga
    21901 cgagcatgcg ggcaagctcg gccgcgtact tggcggtgac gaactcctcg ctcatgttgg
    21961 agatcacgat ttcctgctgc gtggcgacgc ggcggggctg catcggctgg gccttctcgg
    22021 tctcataggt gagttcgtcg atgtagccga gatcgcgcat gcgtccgagc acgtagttct
    22081 tgcgcatggt ggcgcgcgtc ggattgacga tcgggttgta ggccgaaggc gcgacgggaa
    22141 gacccgcgag cgtggcggcc tcgccgatcg agatctggtc gagcgagcgg ccgaagtagg
    22201 ttcgtgcggc gctctggaag ccgtaggcgc gctgacccag atagatctgg ttcatgtaga
    22261 tctcgaggat ctggtccttc gtaagcgttt tctcgatctt gaaggacatc gcgatctcgt
    22321 agagcttgcg ggtgtaggtg cgctcggacg agaggaagaa gttgcgggca acctgctgcg
    22381 tgatggtcga gccgccctgg ccgcggcggc cggtcagtac gttggagagt gcggcgcgga
    22441 tgatgccggt gatctcgatg ccggggtgtt cgtagaagcc gtcgtcctcg gcggcgagaa
    22501 tggcgtgctt gacgtggtcg ggcacttcgg agaggcgcac gaagtcgcgg cgctcctccc
    22561 cgaactcgcc gatgagcttg ccgtcggcgc tccagacgcg aagcggcacg cgcggacgat
    22621 agtcggcaag cgtgtcgatc ggggggagct gcctgtagat gaaggcgaag acgaagacgg
    22681 cgagcagaac gccggaggcg atgcctgcag ccgcaatgcc gaagagccag gccagaaccg
    22741 aacccgcgcg cggggatttg cgcgcaggct tctttctggc ggcggtagtt cgtttggtct
    22801 gggtcacgtg gatgtgttgt cgtgaatgtg tggggccgaa agagaaacac ggccgtcatg
    22861 cgcggccgtg tcggctgaat gtgacgattc taatcgagaa ggcggcgggg cggcggtcac
    22921 ggagggcatt gaaccgttca gggagcaggg tcttttgtat ggaaatgcaa ggtgcgggga
    22981 ggcaggagtc gctgcggttt ttgaacgcag gcagtcttcc cgtccgcatt tgttgtgtag
    23041 tttcttcgcg acatggtccc tccgtcagac cgtagtcggg ccggttttcg ggcgataatg
    23101 tacaaatttg gggaaacgcc tctgggaata ataaaatgca ggaatccgtt ttcagcatgc
    23161 ccgtcgttgt cggagagtcg tccatcgatc tgctcggaca cgtcaacaat cgcgagtaca
    23221 tgcgctggat ggaggcggcg gccaccgcgc acgcagcctc cctcggttgg agcttcgatg
    23281 cccttcggtc ggccggccgt gtgtgggtcg tcaggcagca ctggatcgaa tacctgcgct
    23341 ccgcgcgtct gggcgacgaa cttgtcgttg cgacctgggt ggagagcgtc aggcgcctgt
    23401 cgtccctgag gcgctatgca gtgcttcgca acggcgagct tctgaccgtc ggcgccactg
    23461 aatgggtgct cgtggactac gaaaggcgca cgcccgtgca gatccccgag gagatgcgcg
    23521 cgtcgttcag gctcgttgcg cccgacgcgg aggttctcag ggacatgggc atccggcgga
    23581 tggtccgctt tgcgccgagt cccggactcg ccgcggcgtc caaatgagga attatggaat
    23641 ataccgtcaa aattgaagat gtcacattcg gctatggcga tcgcctgatc ctgcagaacg
    23701 tgtccatgaa gttcggcgcc ggcaaggtcg tcgcgatcat gggggggtcg ggcatgggca
    23761 agacgacgct gctcaagctg atcggcggcc tgctgacgcc tcagggcggc agggtcgagg
    23821 tgacgggcga aacgctcgat ccctcgaacc agaagcgcct ctaccgcatc cgccgccgca
    23881 tgggcatgct cttccagttc ggtgcgcttt tcaccgacct ttcggtcttc gacaacgttg
    23941 ccttcccgat gcgcgagcag acggatctcg acgaggagac cattcgggat ctggtgctca
    24001 tgaagctcaa
//
LOCUS       DBMDTI010000006        22334 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_788_length_22334_cov_2.944182, whole genome shotgun sequence.
ACCESSION   DBMDTI010000006 DBMDTI010000000
VERSION     DBMDTI010000006.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 22334)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22334)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22334
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_788_length_22334_cov_2.944182"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="ACZPB6_00995"
     CDS             <1..806
                     /locus_tag="ACZPB6_00995"
                     /inference="COORDINATES: protein motif:HMM:NF020339.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GT-D fold domain-containing glycosyltransferase"
                     /protein_id="HIR1183088.1"
                     /translation="ALETIDLLKKNDKLSLVRFGDGEFDIIAQKRGPNYQRTSPRLRE
                     ALLKILNSRSICVAVPEALSVDVLTPENSTNSHLRFWRTYVVLRAQLFRHILDSTRLY
                     GDACVSRPYTNSQDDQLATQVFRQIRTLWANKNVVVIEGELTRFGVGNDLLNDAASVK
                     RIIGPAKNAFEKCDQLLEEARKQPEDTIFIVALGPAAKIIAYELSNQFKTLDLGHLDI
                     EYEWYLRKMKEGGSVPNKYVNEARVNFDSESEPFDPSLYESQIIAKFLN"
     gene            complement(869..1129)
                     /locus_tag="ACZPB6_01000"
     CDS             complement(869..1129)
                     /locus_tag="ACZPB6_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR1183089.1"
                     /translation="MSHRLTIKGQVTIPKEIRDFLGLEEGCSCVEFVVEADGSVSVRK
                     AGEKPRSSRSSRTSSVQTVRKPVHGVRTRAHGGVLALLAGGL"
     regulatory      complement(1271..1403)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            complement(1494..2945)
                     /locus_tag="ACZPB6_01005"
     CDS             complement(1494..2945)
                     /locus_tag="ACZPB6_01005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1183090.1"
                     /translation="MSSFAQFNLDSRIMSAIERIGYKEPTPIQLQAIPVVMKGGDVMG
                     AAQTGTGKTASFGLPLIARILPHANTSMSPARHPVRALILTPTRELADQVSANMTQYA
                     ADTPLRVGVVYGGVDIRPQSDMLRRGVEILTATPGRLLDHIEQRATNLSQVEVVVLDE
                     ADRMLDMGFLPDISRILKLLPANRQSLMFSATFSPDIARLAKNFLKPDPFMIEVARQN
                     QTADTVTQEVFQVADREKTDVLIDILKTRGPENKPLEQVIVFVNAKITCRRLARTLER
                     VGINADAIHGDKTQEERQAALDGFKSGAIHVLVATDVAARGLDIKELPCVINYDVPFS
                     AEDYVHRIGRTGRAGSHGLAIMLATREDGRLVDAIEKLTKQTFEQKPLRPLSRQARSE
                     MRRASGERPERRGEREIERVDTPEDERLAKERARAYVPPSHRNRDPIFDTPYRERSGA
                     SGKPEELRVFERETVRKPRQAAVLLCGFPRRRD"
     gene            complement(3155..5107)
                     /locus_tag="ACZPB6_01010"
     CDS             complement(3155..5107)
                     /locus_tag="ACZPB6_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1183091.1"
                     /translation="MKTNEDAFENDDDIDEMEEDSSIDDEAAELDKWEDEGSEEQGSA
                     SGSRRSVTAGNGYGALVRLGRSRGWVTVEEINDHLPESAVRTAENVQEITEALGRLSI
                     KVFETRPDEDDLLITGNGVDEAEDIDEDDAAAMLTPEESAGLSKDPLRAYLRGVGSHK
                     LLTRAGEIEVAKSIEMFTGRLVSTLVQYPMAVAELCKMAETLKDTSVSVDTLVDGFTD
                     NLEEVDAVSSDGDDIATDIGAAAMTADQLEEMRARVLDIFENCGRHLETVRANFGPKG
                     DAEAYKAACAAIAELLAPVRFSVKVVTQLSEDISKHMDEVTTSLKNVRRVLADQCRMP
                     QRATLDVINGDPTADGLFEAIAESETPWAPAVARNLGVLHEEQRHLRGFEERAMMTIA
                     SQRELARLLKLAQTNLMQAKARMIEANLRLVISIAKGYVNRGLAMTDLIQEGNLGLMK
                     AVDKFEYRRGYKFSTYATWWVRQSVTRAVADFGNTIRIPVHMTESYNKIRRHKQHFLQ
                     EHGRQPTEQELADLAELPLAKVQLLTQAMRGVESIDAPIGDDEDATKLDFVRGDEHDD
                     PSRAFQDRSMIECIKKSLDDLAPREAQVLRLRYGIGTNKDHTLEEVGHALGLTRERVR
                     QIESAAIRKLRSPEFQERLRDYLTEN"
     gene            complement(5627..8089)
                     /gene="rpoD"
                     /locus_tag="ACZPB6_01015"
     CDS             complement(5627..8089)
                     /gene="rpoD"
                     /locus_tag="ACZPB6_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR1183092.1"
                     /translation="MAVSKKTTVNEAAQDETEQAPQTVAPKRRRAPAKRAEKEVATLE
                     TAGLKLSVEAPEEAEAPKTKPVRRRTRKTAEAAEAAPAVEEAAPAAEEEAAKPRRTRR
                     KKAGTEEKEAGAAKAAPKRRKTASKKSKAKGRSDEDDLAEYDDDGTGDFDAVLDDDVD
                     YEEDIPDLPDDLSEEDEADEVGEAETDAQIGRRKRKARSARDKKALLNGYGSDEEDST
                     EDRRSKLFTLIRMGRERGYVTYGEINDNLPNSLIDDDAIETVVAILSNLHIQVFESAP
                     DEDTLAMMGNESVASEEDADAEAEAALSTVDSEFGRTTDPVRIYMREMGSVELLSREG
                     EIEISKRIEDGLKHMVLAISRCPVTVAAIVGYAHEIREGTLPIDDIVDGLVAADDTGN
                     VLGHNDDETDMGASAMTVDQLEDMKQRSLAIFAVVEEHFNTLKTVVREEGYGSDRLDP
                     LKDSIQRELMAVRFTAKTADKLCELLRTTIDEVRTRQKTIYNELVRRVGLDRTWFLAT
                     FADHATDLDWMSAIVRDNPEKAAYLEHLRPTVEEEQRKLAELVSNACMTVPELFDTYK
                     QMATGEAKARNAKREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNVGLMKAVDKFEY
                     RRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINRMNRITRQILQETGVEPD
                     SRELAKLMELPEDKILKIMKIAKEPISMETPIGDDDDSHLGDFLEDTQTVAPAEAIQN
                     TSLSETVRQVLDSLSPREAKVLRMRFGIEMQSDHTLEEVGKQFDVTRERIRQIETKAL
                     RKLRHPSRADKLRSFVEGDGKD"
     gene            complement(8575..9321)
                     /locus_tag="ACZPB6_01020"
     CDS             complement(8575..9321)
                     /locus_tag="ACZPB6_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1183093.1"
                     /translation="MIVMRYILGFLFLGGCWHAGALALGPDLLPDPVATIRLFAESLG
                     TPEFWGHILVSLWRLTLGLVAAVAVAFPLGLLLGHCRAADLAGSPLLFITYPLPKIVL
                     LPVFFTLVGLGDASRVLLIALTTGYQVLVIIRAGALSIDPSWAKAFRSMGGTTAEAVR
                     HLYVPAALPALFTSLKVASGTAVAVLFLAESFATTSGLGYLIMDAWGMGDVLSMFNAI
                     LGMSLLGLVIYGVIGLLERVCCPWLFAGRR"
     gene            complement(9318..10118)
                     /locus_tag="ACZPB6_01025"
     CDS             complement(9318..10118)
                     /locus_tag="ACZPB6_01025"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1183094.1"
                     /translation="MQNQREEALEPPGLACNGLTLGYDGRPVIEGLDLSLPPGASLAI
                     VGESGCGKSTLLTALAGLRPAISGTVRWTSPQGGVRSIQDMRTSFVWQHLGLFPWKRV
                     RENLALPLELSAGRCADSAGRVSAMLSELRLSGLESRFPSELSGGQRQRLALGRALIV
                     QPQVLFMDEPFSALDALLRARMQDFLTALRRRHPCSVILVTHDISEAVALGSHVLMLA
                     PHGRRAPECFENPAYSQELGCGDRASPAFYDTVRRIHAGLAAASGEDE"
     gene            complement(10132..11073)
                     /locus_tag="ACZPB6_01030"
     CDS             complement(10132..11073)
                     /locus_tag="ACZPB6_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1183095.1"
                     /translation="MKKTLWAALLAAGTMVSAAVDAEPLRIGVLPAADAIVIHAAADE
                     KLFKARGLDVEVIPFKSALELGAAMRAGRLDGHFGDLMNVFTQNERGVPQAVILTTTH
                     TSRAQRAFGLVVAPAAAEKIRSLKDLDGTETAMSSATIIDYLLDRMKAEEKLSDGALR
                     NLEVKQIPIRLQMLQTGKAATAMLPEPLVSVVEAKGGRVIWDDRGLNEALAVVALKKD
                     RLDDKTVAAFRGAVADAARLIESEPDRFRAVMVKKGLLPAPVAQNYTMVRFSMFGTND
                     GLPPLPSAEDVRRVGEWMVGKNMIKAVPAYDSVVIRP"
     gene            complement(11127..13415)
                     /gene="dnaG"
                     /locus_tag="ACZPB6_01035"
     CDS             complement(11127..13415)
                     /gene="dnaG"
                     /locus_tag="ACZPB6_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR1183096.1"
                     /translation="MIPEGFITGLLERADIVDVINRYVPLKKAGRNWMCCCPFHKEKT
                     PSFSVSQTKQFFKCFGCGEAGNAIGFIMKYEGLEFPDAVRKLAGYYSMEVPEDRSPAK
                     IKAREKARTLTDYMGEAAAFYTEQLRRNAGASDYLKNRCISAATAAKFGLGYSPDDWH
                     PLQTLFGEKYSDRALEGCGLVNVRNDRRYDAFRGRIMFPIRNAKGLVIGFGARTMNGD
                     EQPKYLNSPETPIYHKGSELYGYFEGREAIYGKGRAIVCEGYMDVIQLSQAGFEEAVA
                     ALGTSITPEHVRKLFKLTDSVYFSFDGDAAGRKAARRALEAALPVITDVQKAGFIILP
                     PEHDPDSLIKAEGAEGFERQIEKAYGLTDFMKKLLLEGKELMYAEERAKLVAEAKPWV
                     LSMQHAPILRLAFIRELAGIARLQADDIERQYGLAGAAPSRPSFDGGRQQGRGGFARR
                     RDDRFPRWPAKPAPEPRVRVKDVRERMLQCLLSYPHLVSEFSHSIEEEFLSSTHPAAE
                     RIIEVWRAAAAEDEEGGCVNPASLLMRLGESASLSYYEELLTEELSTGTPEEGARLEV
                     RKAFIELELERTKARIEMLAHDAARDMDAMRRLNDRRAELQRLADEARLAEREYRLRI
                     ENQARFESQRAAEKPSGGEFAPLSSNPIVAQLQRFLRGDTGTSSVSGAQAAPQGASRV
                     STSAVREALAASAAGRTAGDFTAFAESLPPELSDEKVDFVPDGMKEYDVSGMPPDVPF
                     DDEAPYRYDDAGDPDADLNVGL"
     gene            complement(13632..14075)
                     /locus_tag="ACZPB6_01040"
     CDS             complement(13632..14075)
                     /locus_tag="ACZPB6_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR1183097.1"
                     /translation="MIKDRIREDMKAAMRAHDAAGLSTIRLLLAAIKQREVDEQIEAD
                     DALILSVIAKMVKQRQDSVQQYLAGDRQDLADKESVEIKVLEAYLPKGLSDEEIAAVI
                     DAAVAEVGATGMAAMGKVMAIVKPKVTGRADMGKVSALVKAKLTA"
     gene            complement(14189..14401)
                     /gene="rpsU"
                     /locus_tag="ACZPB6_01045"
     CDS             complement(14189..14401)
                     /gene="rpsU"
                     /locus_tag="ACZPB6_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR1183098.1"
                     /translation="MPTIRVKENEPFEVALRRFKRTIEKSGLLTELRAREFYEKPTAE
                     RKRKKAAAVKRHYKRLRSMMLPKKLY"
     gene            14633..15664
                     /gene="tsaD"
                     /locus_tag="ACZPB6_01050"
     CDS             14633..15664
                     /gene="tsaD"
                     /locus_tag="ACZPB6_01050"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR1183099.1"
                     /translation="MLVLGIESSCDETGAALISDKDGLLADALYSQIDMHRAYGGVVP
                     ELASRDHIRRAVALVDEVLVKAGKTRGDIDAVAVTEGPGLAGALLAGAGVAHAIGWAL
                     DVPVLGVHHLEGHLLAARLSDPAPKFPFLALLVSGGHTQIMKVEKFGSYELLGQTLDD
                     AAGEAFDKTAQLLGLPYPGGPAVSALAEEGEPGAIELARPMIHAPNLDMSFSGLKTSV
                     LTAVQSAPDAGDEVFRRNLARGFVDALVDVLAAKLVRAMKQTGLRNVVVAGGVSANRQ
                     LRQRLTAEVRRRRGTIFYPPMRLCTDNGAMIAAAGLARLEGMTAEELEALKAKRSFGV
                     SPRWQLSSL"
     gene            complement(15766..16260)
                     /gene="ybaK"
                     /locus_tag="ACZPB6_01055"
     CDS             complement(15766..16260)
                     /gene="ybaK"
                     /locus_tag="ACZPB6_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIR1183100.1"
                     /translation="MSSKVKHQSLTPATQWLRQHDIPFEEKSYEYVEHGGTALAASSC
                     GIDHHHVIKTLIMEDEHARPLVILMHGDCEVSTKNLARQIGAKHVAPCKPEQAQRNSG
                     YMVGGTSPFGTRKKMPVYVQKTILELDHIFINGGRRGYQVGIDPKVLVDVLGGIPVDC
                     ANEE"
     gene            complement(16396..17337)
                     /gene="ispH"
                     /locus_tag="ACZPB6_01060"
                     /gene_synonym="lytB"
     CDS             complement(16396..17337)
                     /gene="ispH"
                     /locus_tag="ACZPB6_01060"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR1183101.1"
                     /translation="MKVLLAQPRGFCAGVTRAIAIVERALAIYGAPIYVRHEIVHNRT
                     VVNSLRERGAVFVDNLAEVPEGATVVFSAHGVPRAVSEEAERRGFRVFDATCPLVTKV
                     HREVARMRAEGRTVLMIGHAGHPEVEGTMGQVDGGIELVENTADVAALPYTNDDSLAY
                     VTQTTISADDSQAVIDALRHRFPFIQEPKRQDICYATTNRQEAVKLLARSGVDIVLVI
                     GSATSSNSNRLREVAEREGARAYLIDTAEHIDPKWFEGVACVGITAGASAPESLVQGV
                     LRYLEANLNAEHAVALEGVEENVVFPLPKGLREEDAD"
     gene            17724..17957
                     /gene="rpmB"
                     /locus_tag="ACZPB6_01065"
     CDS             17724..17957
                     /gene="rpmB"
                     /locus_tag="ACZPB6_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR1183102.1"
                     /translation="MARVCQVTGKAPMVGHHVSHANNKTKRRFMPNLQYRRFWVESEN
                     RWVRLRLTTAGLRTVDKIGIDAVLADLRARGEI"
     gene            17974..18141
                     /gene="rpmG"
                     /locus_tag="ACZPB6_01070"
     CDS             17974..18141
                     /gene="rpmG"
                     /locus_tag="ACZPB6_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR1183103.1"
                     /translation="MAKGAREKIKLESTAGTGHFYTTTKNKKTSSGKMEISKFDPVAR
                     KHVIYKETKLK"
     gene            complement(18354..19826)
                     /locus_tag="ACZPB6_01075"
     CDS             complement(18354..19826)
                     /locus_tag="ACZPB6_01075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR1183104.1"
                     /translation="MAETKFPKRRAFAMQKPRKTERTESPRRTERQRAADKRRREEAR
                     AMGGYAKGPKTPEKRMTALQPGQVRITGLIVDELTRALGVILKLDGPADVLMKLFFRS
                     NPRLGMRDRGIIAEGIYYALRHLGTLSWAMHPVPPARAPRLVALATLARQHGIESLPP
                     ASIGREEGPLKNVLAAAFSKAPPHIQAELPEWIYSLLEAQYDDTKELYASMLEGAPLD
                     LRVNLLKASREEVLQELAANGVEAYPTPFSPDGVRLPTKPGLTQWPIYKEGKVDVQDE
                     GSQLIARLLTPRRREMICDFCAGAGGKTLAIGALMRSTGSLYAFDVNEKRLAGLTPRM
                     RRAGLSNVHPIAIRSERDERIRRLQGKFDRVLIDAPCTGTGTFRRNPDLKWRLAPSEL
                     DRINKIQKDVLEHASRLVKKGGRMVYATCSMLRRENQEVVEAFLEAHPEWHLVPAADV
                     FAQQGIQFDASQWERFGSYFQMLPHVNSTDGFFAAVLQRD"
     gene            complement(19944..20594)
                     /gene="purN"
                     /locus_tag="ACZPB6_01080"
     CDS             complement(19944..20594)
                     /gene="purN"
                     /locus_tag="ACZPB6_01080"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR1183105.1"
                     /translation="MQNIVILISGRGSNFEAILRTSREENWESACNARIAAVISNRPQ
                     AAGLDIARAAGIEAVAMDHKAYDSREAFEAELADIISRYDPAVIVLAGFMRVLTEGFV
                     SRFEGKILNIHPALLPLFPGLNTHQRAIDAGCRVHGSTVHFVTAVLDGGSIIGQAVVP
                     VLPSDDADALAHRGLVYEHQLYPKCVRAILEGRVRCEDGRTVCNDETARSLTIFGC"
     gene            20700..21680
                     /locus_tag="ACZPB6_01085"
     CDS             20700..21680
                     /locus_tag="ACZPB6_01085"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR1183106.1"
                     /translation="MHVFRGLPSPLQRRPSAVAIGNFDGVHLGHQALLEYVKEAAQAR
                     GLVPSVVTFEPHPKDFFGRGGEVARISTLYDKVNAILGTGIERIYILPFNSRLACMPA
                     ETFVRDVLLKGLDACWMSVGKDFRFGGDRAGSVELLEELSRTLPYELYVSPTFYHKEA
                     RVSSSRIREALAVGDLYEAALMLGRPYSMTGRVIHGAALGRTLGFPTLNMAPIPPGSH
                     SRPAVTGVFAVRIHGLGQAVQNGVASIGVKPTVATDGRWLLETHVFDWSGSAYGRIVK
                     VEFVERLRGEKKFSGLDELKAAIESDADHARRILGCVRPQADVPLQGMNP"
     gene            21721..>22334
                     /locus_tag="ACZPB6_01090"
     CDS             21721..>22334
                     /locus_tag="ACZPB6_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1183107.1"
                     /translation="MAKEAGSAADTKKYPLNLPDTPFPMRGNLPKREPEWIREWDEKH
                     IYERIIDARRDAKKFILHDGPPYANGNIHVGHAVNKILKDMILKEKTLEGFQAPYVPG
                     WDCHGMPIEVQIEKMHGKNLPVDKMQSLARAYALEQSKLQLADFKRLGVLGDWDNPYR
                     TMKFKNEAEEIRALAAVVKKGYVYRGLKPVNWCFDCQSALAEAEV"
BASE COUNT         4212 a         6946 c         6907 g         4269 t
ORIGIN      
        1 cggcgctgga aaccattgac ctgctgaaaa agaacgacaa gctttccctt gtgcgttttg
       61 gcgacggtga gttcgacatc atcgcccaaa agaggggacc aaactaccag agaacctctc
      121 cgcgcctcag ggaagcgctt ttgaagattt tgaacagcag gagcatttgc gtggccgttc
      181 ctgaggctct gtcagtggat gtgctgacac cagaaaacag caccaattcc cacttgagat
      241 tctggagaac ctatgtcgtc ttgagagctc agctgttccg ccacattctc gattcaaccc
      301 gtttgtatgg ggatgcctgc gtttcaagac cctataccaa tagtcaagat gaccagcttg
      361 ctactcaagt ttttaggcag atccgaacac tctgggccaa taaaaatgtt gtcgtgatcg
      421 aaggggagct tacccgtttc ggagtcggaa acgatcttct caacgacgca gccagcgtca
      481 aaagaatcat cggccctgcc aaaaacgcct tcgaaaaatg cgatcagctc ctcgaagaag
      541 cccgcaaaca gcccgaggat acaatcttca tcgttgcact cggtcccgca gccaaaatca
      601 tcgcctacga actctccaac cagttcaaaa cccttgatct ggggcatctg gacattgaat
      661 acgaatggta tctgagaaaa atgaaggagg gcggcagcgt ccccaacaaa tatgtcaatg
      721 aagccagagt caacttcgat tccgagtcag aacctttcga cccaagtctt tacgaatctc
      781 aaatcatcgc caagtttttg aactagacga ctttcgcctc cgctggttgt gcgcctgcag
      841 aggctttctt ttgaagtaga gactcgcttc aaaggccgcc cgcaagcagg gcgagaacgc
      901 cgccatgggc gcgcgtgcga acaccgtgca cgggtttgcg cacggtttga acggaggaag
      961 tgcgggagga acgactggaa cgaggctttt cgcccgcctt gcgcacgctg acgctgccgt
     1021 cggcttcaac gacgaactcc acgcaggagc agccttcctc gagtccgaga aagtcacgaa
     1081 tttccttggg gatcgtgacc tgccccttga tggtaagacg atgcgacata tgcttctcct
     1141 tcatgcagat aaatgttgat ggatgtgcat gccgcggaac tttttcggtc aggggcctca
     1201 tcctcgatga agctccggtc ctgatgcgaa aaagcctccc gcagcgtaca cgcatgtcga
     1261 gaggcattgc cattctccgt atgtttgtcg gtgcgcgatg agctaccggc cacatggcct
     1321 cccgcggagg ccaccacctt gcccggaggc gggcaggttg gcgtgagggc ttttaggaaa
     1381 gtcctcatct cttcatgctg tcgaattaat atacacgtcc gaggcacttc gtcgcaacac
     1441 gcctaccatg gataggtgat ggacaaagtg cctagacgag ctgatcattt cgatcaatcg
     1501 cgtcgacggg gaaagccgca aagcagaacc gccgcctgtc ggggctttcg aacggtctcg
     1561 cgctcgaaga ctcgaagctc ttcgggcttg cctgaagcgc cgctgcgctc acgatagggc
     1621 gtgtcgaaga tcggatctcg gttgcggtgg gagggcggca catatgcacg ggcacgctcc
     1681 ttggcgagac gttcatcctc gggcgtatcg acgcgctcga tctctcgctc gccacgtcgt
     1741 tcgggacgct ctcctgaggc gcggcgcatt tccgaacgag cctgacgcga gagcgggcgc
     1801 aggggcttct gttcgaaggt ctgcttcgtg agcttctcga tggcgtcgac aagacggccg
     1861 tcttcgcgcg tcgcgagcat gatggcaagg ccgtgggaac cggctcgacc ggtacggccg
     1921 atgcggtgca cgtagtcctc ggcgctgaac ggaacatcat agttgatgac gcagggcaac
     1981 tccttgatgt ccagacctcg cgccgcgacg tcggtcgcaa cgagaacatg gatggcgccc
     2041 gacttgaagc cgtcgagcgc cgcctgacgc tcctcctgcg tcttgtcgcc gtgaatggcg
     2101 tctgcattga tcccgacgcg ctcaagcgtg cgggcaaggc gacggcaggt gatcttcgcg
     2161 ttgacgaaga cgatgacctg ctcgagcggc ttgttttcgg gacctcgcgt cttgaggatg
     2221 tcgatcagca cgtccgtctt ctcgcgatcc gcgacctgaa agacctcctg agtaaccgtg
     2281 tcggccgtct gattctgacg ggcgacttcg atcatgaacg gatcgggctt gaggaagttc
     2341 ttcgcgagcc tggcgatgtc gggcgagaac gtagccgaaa acatcagact ctgacggttg
     2401 gccggcagaa gcttgaggat gcggctgatg tcaggcagga agcccatgtc gagcatgcgg
     2461 tcagcctcgt cgagcacgac gacctcgacc tgagagagat tcgtcgcgcg ctgctcgata
     2521 tggtcaagca gacggcccgg cgtcgccgtc agaatttcga cgccgcggcg gagcatgtcg
     2581 gactgcggac gaatgtcgac gccgccgtag accacaccga cgcgcaacgg cgtgtccgcc
     2641 gcatactgcg tcatgttggc agagacctga tcggccagct cgcgcgtagg cgtcagaatg
     2701 agcgcgcgca cgggatgacg ggcgggcgac atcgaggtgt tcgcatgcgg aagaatgcgg
     2761 gcgatgagcg ggagaccgaa gctcgccgtc ttgcccgtgc ccgtctgggc tgcgcccatc
     2821 acgtctcccc ccttcatcac cacgggaatg gcctgaagct gaatcggcgt cggctccttg
     2881 tagccgatgc gttcgatggc ggacatgatg cgggagtcga gattgaattg tgcgaacgat
     2941 gacatgctgc tggcgttctc ttggaatggc tcttgagtgt gggcggcgtc gcaagggcac
     3001 ttgctgaacg cctgaaaacc gttattttag cgggattccg cacgtggcgc agttttcggc
     3061 cacccgcatc agggcggcgc gtcctcccga aaacgcgaaa aggctcccga aggagccttt
     3121 tcaggcgact ccgcacgagg cggagtcaag gggatcagtt ctcagtcagg tagtcgcgca
     3181 gacgttcctg gaactcggga ctgcgaagct tgcggatggc cgccgactcg atctggcgga
     3241 cgcgctcgcg cgtaagaccg agcgcatggc cgacttcctc aagcgtgtgg tccttgttcg
     3301 taccgatgcc gtagcgcagg cgaagcacct gagcctcacg gggagcgaga tcgtcaagcg
     3361 acttcttgat gcattcgatc atcgaacggt cctggaaggc acggctcgga tcgtcatgtt
     3421 catcgccgcg cacgaagtcg agcttcgtcg catcctcgtc atcaccgatt ggtgcgtcga
     3481 tggactcgac gccgcgcata gcctgcgtga gaagctgcac cttggcgagc ggaagctcgg
     3541 caagatcggc aagctcctgc tcggtcggct gccggccgtg ctcctgcagg aagtgctgct
     3601 tgtgacggcg gatcttgttg taggactccg tcatgtggac cggaatgcgg atggtgttgc
     3661 cgaagtcggc cacggcacgc gtgacggact gacggaccca ccaggtcgca taggtcgaga
     3721 acttgtagcc tcggcgatac tcgaacttgt ccacagcctt catcagacca aggttgccct
     3781 cctgaatcag gtcggtcatc gcgagaccgc ggttgacata gcccttggcg atcgagatca
     3841 ccagacgcag gttcgcctcg atcatgcggg ccttggcctg catgagattc gtctgcgcaa
     3901 gcttgagcag acgggcaagt tcgcgctggc tggcgatcgt catcatcgcg cgctcctcga
     3961 aaccgcgcag atgacgctgc tcctcgtgca gaacgccaag gttgcgcgca acggcaggcg
     4021 cccacggcgt ctcgctctcg gcgatggcct caaaaagccc gtcggcggtc ggatcaccgt
     4081 tgatcacgtc aagcgtggcg cgctgcggca tgcggcactg gtcggcaaga accctgcgga
     4141 cgttcttgag ggacgtcgtc acctcgtcca tgtgcttgga gatgtcctcg gagagctgcg
     4201 tgacaacctt cacggagaag cgcacgggcg cgaggagctc ggcgatggcg gcgcaggcgg
     4261 ccttgtaggc ctcggcgtcg cccttcgggc cgaagttggc gcgaacggtc tcaagatgcc
     4321 tgccgcaatt ctcgaagatg tcgagcacgc gagcgcgcat ctcctcgagc tggtcggccg
     4381 tcatggcggc ggcgccgatg tcggtggcga tgtcgtcgcc gtcagaggag acggcgtcca
     4441 cttcctcgag gttgtccgta aagccgtcga cgagcgtgtc gacggagacg gacgtatcct
     4501 tgagggtttc ggccatcttg caaagctcgg ccacggccat cggatactgc acgagcgtcg
     4561 agacaagcct gcccgtgaac atttcgatcg acttggcaac ctcgatttca cccgcacggg
     4621 tgagaagctt gtgcgagccc acgccgcgca gataggcgcg gagaggatcc ttggagaggc
     4681 ctgcggactc ctcgggcgtc agcatggcgg cggcgtcatc ctcgtcgatg tcctcggcct
     4741 cgtcgacgcc gttgccggtg atgagaaggt cgtcttcgtc gggacgcgtc tcgaacacct
     4801 tgatcgaaag gcggccgagc gcctccgtga tctcctgcac gttttcggcc gtacgcacgg
     4861 cgctttcagg caggtggtcg ttgatttcct caaccgtcac ccagccgcgg gagcggccga
     4921 gacgcaccag agcgccgtag ccattgcccg ccgtcaccga acggcggctg ccagaagccg
     4981 aaccctgttc ctcggaacct tcatcctccc acttgtcgag ttctgcggct tcatcgtcga
     5041 tggaagagtc ctcctccatt tcgtcgatgt cgtcatcatt ctcaaaagcg tcttcgttcg
     5101 tcttcatctg ttccaatctc tcgttggcgg ggctcttatt gttgcaattg ccccgcttct
     5161 tcctaatttc tccggaccgc ctcggcgccg cccttctccc gcatcctcct ctcgtccgcc
     5221 cgaatcattc tgtcttctcg gggcctgccg actatcggcc tgggagccat gcatccaggg
     5281 aaaagcagcc ggtcatcctc gcagcccggc gctgcatcga ttatttttcg tcctacgtca
     5341 gcgcgcttcg tgccgtcgtt gcctgatctc ccgttctctt ccggagcaac gcgatcctgc
     5401 gcctccggcg gtccagaccg cacgacgcct tccgcgacag tgtcggcaga caccgtcatg
     5461 ggcgaaaaag gcggatagct taacactgcg ccatactctt ttcaagcaga aaaagcaaca
     5521 aacggcgaaa cgccgcccgt cgccctcccg tcagggcctc cgagcggcac gggcgccgca
     5581 tttgaaaacg gggaacggcc ttagccgctc cccgtcatgc aaggcatcaa tcctttccgt
     5641 cgccctcgac gaagcttctc agcttgtcgg ctctagacgg atgacgaagc tttctgagcg
     5701 ccttcgtctc gatctggcgg atgcgttcgc gcgtgacgtc gaactgcttg ccgacctcct
     5761 caagcgtgtg gtcggactgc atctcgatgc cgaagcgcat gcgcagaacc ttggcctccc
     5821 tcggggaaag actgtcgaga acctgacgga cggtttcgga gaggctcgtg ttctgaatgg
     5881 cctcggcggg cgccaccgtc tgcgtgtcct caaggaagtc gccgagatgg gaatcgtcat
     5941 cgtcgccgat cggtgtctcc atcgagatgg gctccttggc gatcttcata atcttgagaa
     6001 ttttgtcctc gggcagctcc atcagcttcg caagctcgcg ggaatcgggc tcgacgcccg
     6061 tttcctgcag gatctggcgc gtgatgcgat tcatgcggtt gatcgtctcg atcatgtgca
     6121 cgggaatgcg gatcgtacgg gcctggtccg cgatcgaacg cgtgatggcc tgacggatcc
     6181 accaggtggc ataggtcgag aacttgtagc cgcggcggta ctcgaacttg tcgacggctt
     6241 tcatgaggcc gacattgccc tcctgaatga gatcgaggaa ctgcaggccg cggttcgtgt
     6301 acttcttcgc gatcgagatg acgagcctga gattggcctc ggtcatctca cgcttggcgt
     6361 tgcgtgcctt ggcctcgccc gtcgccatct gcttgtaggt atcaaagagc tcgggcacgg
     6421 tcatgcatgc attcgagacc agttcggcaa gcttgcgctg ctcctcctca accgtgggcc
     6481 tgaggtgctc gagataggcg gccttttcgg gattgtcacg tacaatggcg ctcatccagt
     6541 caagatcggt cgcatgatcg gcaaatgttg caaggaacca cgtgcggtca agcccgacgc
     6601 ggcgcacgag ctcgttgtag atcgtcttct ggcgggtgcg cacctcgtcg atcgtcgtgc
     6661 gaagaagctc gcagagcttg tcagcggtct tggcggtgaa gcgcacggcc atcagctcgc
     6721 gctggatcga atccttgagc gggtcgagcc tgtcgctgcc gtaaccctcc tcgcggacaa
     6781 cggtcttgag cgtattgaaa tgctcctcca cgacggcaaa gatcgcgagg ctgcgctgct
     6841 tcatgtcctc gagctggtcc accgtcatgg cggaggcgcc catgtcggtc tcatcgtcgt
     6901 tgtggccgag cacgttgccc gtatcatccg cagcaacgag gccgtcgacg atgtcgtcaa
     6961 tgggcagcgt gccctcgcgg atttcatgcg catagccgac gatcgccgcc acggtgaccg
     7021 ggcagcggga gatggcgagc accatgtgct tcaggccgtc ctcgatgcgc ttggagattt
     7081 cgatttcgcc ttcacgggaa agaagctcca ccgagcccat ctcgcgcatg tagatgcgca
     7141 cggggtcggt cgtgcggccg aattcgctgt cgacggtcga gagcgcggct tcggcctccg
     7201 catccgcatc ctcctcgctg gcaacgctct cgttgcccat catggcgagc gtatcctcgt
     7261 cgggagcgga ttcaaagacc tgaatatgca gattcgaaag aatcgccacg acggtctcga
     7321 tcgcatcgtc atcgatcagg ctgttgggca ggttgtcgtt gatttcgccg taggtgacat
     7381 agccgcgctc gcgccccatg cggatgagcg tgaagagctt ggaacggcgg tcctcggtgg
     7441 agtcctcctc gtcggagccg taaccgttga gaagcgcctt cttgtcgcgg gcgctgcgcg
     7501 ccttgcgctt gcggcggccg atctgggcgt ccgtctccgc ctcgccgact tcatcggcct
     7561 cgtcctcttc ggaaagatca tcaggcagat cgggaatgtc ctcctcgtag tcgacgtcgt
     7621 cgtcaagcac agcgtcgaag tcgcccgttc catcgtcgtc gtattcggcc agatcgtcct
     7681 catccgagcg tcccttggcc ttcgacttct ttgaagcggt cttcctacgc ttcggcgcgg
     7741 cctttgcggc acccgcctcc ttttcctcgg tccccgcctt cttgcggcgg gtgcggcggg
     7801 gcttggcggc ctcctcttca gcggcgggag ccgcttcctc gacggcaggg gcggcttcag
     7861 cggcctcggc ggtcttgcgg gtacggcgac gcacgggctt ggtcttgggg gcctccgcct
     7921 cttcaggggc ctccacggag agcttcagac cggcggtttc caaggtcgcg acctccttct
     7981 cggcgcgctt tgctggggca cgcctccttt tgggagccac cgtctgaggc gcctgctccg
     8041 tttcatcctg agccgcctca ttcaccgtcg tctttttgga tacagccata cctgccctct
     8101 tctgctgaga cggcgcgcat ctctctcacg ccgactccct gcgctgcgca cgactgacta
     8161 ggccgagcgg cacgcggatc aaacacggag acccggaggc ctcccttcgc cgctgcatgc
     8221 ggagcatggc gcccgtcggg cgccctccga cgcatctttt gccctgaatt cttccggtcg
     8281 aaaaactcac ggcaaaatcc gcgtgtcgtc tccacgtgat gtcattcgcc aaaccgagag
     8341 ccgatatgct tttttcaaag gtttcaaaaa agtacgcctt tccgcttgac aaagcaaagg
     8401 acaatgactt aatcattttg ccacaccgcc tcccgaaagt ggaggcggca taatggcaat
     8461 ccctgccttc gaagtcgtgt ttttccgtca gaaagcatgc ttttaatcaa aaaaacggcg
     8521 gaaaattccg ccgtttatga tggtttgtcc gaataggcac cctctgtcgg agccctacct
     8581 cctgcccgca aagagccagg gacagcacac cctctcgagc agtccgatga cgccgtagat
     8641 cacgagacca agaaggctca tgcccagaat ggcgttgaac atggagagca cgtcccccat
     8701 gccccaggcg tccatgatga ggtagccgag ccccgaggtg gttgcgaagc tttcggccag
     8761 aaagagcacc gcgaccgccg tgccgctcgc caccttgagg gaggtgaaga gcgcagggag
     8821 cgctgcagga acatagagat gtcggacggc ctcggccgtc gtgccgccca tcgagcggaa
     8881 ggccttggcc caggagggat cgatgctgag cgcccccgca cggatgatga cgagcacctg
     8941 atagcccgtt gtaagcgcaa tgaggagaac gcgcgaggcg tctccgagcc ccaccagcgt
     9001 gaagaacacg ggaagcaata cgatcttggg cagcggataa gtaatgaaga ggagcggact
     9061 gcccgccaga tccgccgcac ggcagtgtcc gagcagaagt cccagaggaa aggcaaccgc
     9121 gacggccgca acaaggccga gcgtcaggcg ccagaggctc acgaggatgt gcccccagaa
     9181 ttcaggcgtg cctagagact cggcgaagag cctgatcgtg gccacgggat cgggcagaag
     9241 gtcgggtccg agcgcaagcg cgcccgcgtg ccagcatccg cccagaaaga gaaagccgag
     9301 gatgtagcgc atcacgatca ttcgtcctct cccgaagcgg ccgccagccc cgcgtggatg
     9361 cggcgcacgg tgtcgtaaaa ggcgggcgag gccctgtcgc cgcagccgag ctcctgcgag
     9421 taggcgggat tttcaaagca ttcgggcgct ctgcggccgt ggggcgcaag catcaggaca
     9481 tgcgaaccga gcgcaacggc ctcgctgatg tcgtgtgtca ccagaatcac gctgcatgga
     9541 tgcctgcggc ggagcgccgt caggaaatcc tgcatgcgcg cgcgcaaaag cgcgtcaaga
     9601 gccgagaagg gctcgtccat gaagagaacc tgaggctgca caatgagcgc gcggccgagc
     9661 gcaagacgct gccgctgtcc tcccgaaagc tcggagggaa agcgcgactc gagcccagag
     9721 agtctgagtt cggaaagcat ggccgagaca cgcccggccg aatcggcgca gcgtccggcg
     9781 gaaagctcca gtggaagcgc gaggttctcc ctcacgcgct tccacggaaa aagaccgaga
     9841 tgctgccaga cgaagcttgt gcgcatatcc tgaatcgaac ggactccgcc ctgaggagag
     9901 gtccacctga ccgtccccga aatcgcaggc ctcagccccg cgagagcggt gagaagcgtc
     9961 gacttcccgc aaccggactc gccgacgatg gcaagcgagg cccccggcgg caacgacaga
    10021 tccagcccct caatgacggg cctgccgtcg tagccaagcg taagcccgtt gcaggccagt
    10081 cccggcggct caagcgcctc ttccctttga ttctgcatgc gcaccttcga attacggacg
    10141 gatcaccacg ctgtcgtaag cgggcaccgc cttgatcatg ttctttccga ccatccattc
    10201 tcccacgcgg cgcacgtctt cggcgctcgg caggggaggc agaccgtcat tcgtgccgaa
    10261 catggagaag cggaccatcg tgtagttctg cgcaacgggc gcaggtagca ggcccttctt
    10321 caccatgacg gcgcggaagc ggtcgggctc cgactcgatg agtctggcgg cgtccgccac
    10381 ggcaccccgg aaggcggcga cggtcttgtc gtcaagcctg tccttcttga gcgcaacgac
    10441 ggcaagcgcc tcgttgagac cgcggtcatc ccagatcacg cgtccgccct tcgcttcgac
    10501 gacggagacg agcggctcgg gcagcatggc cgtcgcggcc ttgccggtct gaagcatctg
    10561 aaggcggatc gggatttgct tgacctcaag gttcctgagc gcgccgtcgg aaagcttttc
    10621 ctcagccttc atgcggtcga gaaggtagtc gatgatcgtg gcggagctca tggccgtctc
    10681 agtgccgtcg agatccttga gcgagcggat cttctcggcg gcggcaggcg cgacgacgag
    10741 accgaaggca cgctgtgccc ggctcgtatg ggtcgtcgtg agaatcacgg cctggggaac
    10801 gccgcgctca ttctgagtga agacgttcat cagatcgccg aaatgcccgt caaggcggcc
    10861 cgcacgcatg gcggcaccaa gttcgagagc agacttgaag ggaatcacct caacgtcaag
    10921 ccctctggcc ttgaacagct tctcgtcggc cgcggcgtgg atcacaatgg cgtcggcggc
    10981 tggaagcaca ccgatgcgaa ggggctcggc gtcgaccgca gcagaaacca tcgtgccggc
    11041 agccagcagg gccgcccata aagtcttctt caaaatgagc tccttaggga aaagctagtc
    11101 gcgccggcgc gcggcctccc ccgccgtcac agacccacgt tgagatccgc gtcgggatct
    11161 ccggcgtcgt catagcggta gggagcttca tcgtcgaacg gcacgtcggg cggcatgccc
    11221 gagacgtcat attctttcat accatcgggt acaaaatcaa ccttctcatc cgaaagctca
    11281 ggcggcaagc tctccgcgaa ggcggtgaaa tctcccgcgg tacgccctgc ggcgcttgct
    11341 gccagagcct ccctcacggc actcgttgaa acacgggagg ctccctgagg cgcggcctgc
    11401 gcgcccgaga cagaagaagt ccccgtgtcg cccctgagga agcgctgcag ctgcgcgacg
    11461 atcggattgg acgaaagagg ggcgaactcg ccgcccgaag gcttttcagc cgcacgctgg
    11521 ctctcgaacc ttgcctgatt ctcaatgcgc aggcggtact cgcgctccgc aagacgggcc
    11581 tcgtccgcaa ggcgctgaag ctcagccctt ctgtcgttga gcctgcgcat cgcgtccatg
    11641 tccctcgccg catcatgggc gagcatttcg atgcgggcct tcgtgcgctc gagctcgagc
    11701 tcgatgaagg ccttccttac ctcaagacgg gcgccctcct cgggcgtgcc cgtcgagagc
    11761 tcctcggtga ggagctcctc ataataggaa aggctggcgc tctctccgag cctcatgaga
    11821 agggaagcag gattgacgca gcctccttcc tcgtcctcgg ccgccgctgc gcgccagacc
    11881 tcgatgatgc gttcggcggc gggatgcgtg ctcgacagga actcctcctc gatcgagtgg
    11941 ctgaattcgc tcacgagatg aggataggaa aggaggcact ggagcatgcg ctccctaacg
    12001 tccttgacgc gaactcgagg ctcgggtgcg ggcttcgcgg gccagcgtgg aaagcggtcg
    12061 tcacgcctgc gggcaaagcc gccacggccc tgctgccggc cgccatcgaa ggacggcctg
    12121 gagggagcgg cgccggcgag cccgtactgg cgctcgatgt catccgcctg caggcgggcg
    12181 atgcctgcga gctcgcggat gaatgcaagc cgcagaatgg gagcatgctg catcgagagc
    12241 acccaaggct tggcctcggc cacgagcttc gcacgctcct cggcgtacat gagctccttc
    12301 ccttcgagaa ggagcttctt catgaagtcc gtgagtccgt aggccttttc aatctggcgc
    12361 tcgaacccct ccgcgccctc cgccttgatg aggctgtcgg gatcgtgctc gggcggcaga
    12421 atgatgaaac ccgccttctg cacgtccgtg atgacaggca gggccgcctc aagcgcacga
    12481 cgcgcggcct tgcggccggc ggcgtcgccg tcgaacgaga agtagacgct gtcggtgagc
    12541 ttgaagagct tcctgacatg ctcgggcgtg atcgacgtgc cgagtgcggc gacggcctcc
    12601 tcgaacccgg cctgcgaaag ctggatcacg tccatgtagc cctcgcagac gatggcgcga
    12661 cccttgccgt agatcgcctc cctgccctca aaataaccgt agagttcgct gcccttgtgg
    12721 tagatcggcg tttcgggact gttgaggtac ttgggctgct cgtcgccgtt catcgtgcgg
    12781 gcgccgaaac cgatcacaag gcccttggcg ttgcggatcg ggaacatgat gcgccctctg
    12841 aacgcatcgt agcgcctgtc gttcctgacg ttgacaagac cgcacccctc aagcgccctg
    12901 tcggaatact tctctccgaa aagggtctga aggggatgcc agtcgtcggg cgaatagccg
    12961 agaccgaact tcgcagccgt ggcggccgaa atgcagcggt tcttgaggta atcggaggcc
    13021 ccagcgttcc gcctgagctg ctccgtgtaa aaggccgcgg cctctcccat gtagtccgtg
    13081 agcgtgcgcg ccttctcgcg ggctttgatc tttgcgggcg agcggtcctc gggaacctcc
    13141 atcgagtagt agcccgcaag ctttctcacc gcatcaggga attcaagccc ctcgtacttc
    13201 atgatgaagc cgatggcgtt gcccgcctca ccgcagccga agcacttgaa gaactgcttc
    13261 gtctggctca ccgagaagga cggcgtcttc tctttgtgga agggacagca gcacatccag
    13321 ttgcgccccg ccttcttcaa agggacgtag cggttgatca cgtcgacgat gtcggcgcgt
    13381 tcaagcagtc ctgtgataaa gccctcggga atcatggatg gtcttgctag gggaaaaggc
    13441 gtggatcttc cgcccggaag gggcggcggg taggattttg attatagagc cttgagcggg
    13501 ccggctctcg ggtgcctcct gagggcgccc aataaaaaag aaggccgccc aagagaaact
    13561 cttgagcggc attcctgcgt tatgaacgtg cggcttagag cagccgcacc cgccatgcag
    13621 cggacttcga cttaagcggt gagcttcgcc ttgacgagag cgctgacctt gcccatgtcg
    13681 gcacggcccg tcaccttcgg cttgacgatc gccatcacct tgcccatcgc agccatgccg
    13741 gtcgcaccga cttcggcaac ggcagcatca atgacggcag cgatctcctc gtcggaaagt
    13801 cccttcggga gatacgcttc gagcaccttg atttcgaccg attccttgtc agcgagatcc
    13861 tgacggtccc ccgccagata ctgctgcacg gaatcctgac gctgcttgac catcttggcg
    13921 atcaccgaga ggatcagggc atcgtcggcc tcgatctgtt cgtcgacctc acgctgcttg
    13981 atcgcggcaa ggagcaagcg aatcgtgctc agacccgccg cgtcatgcgc acgcatggca
    14041 gccttcatat cttcgcggat tctgtcctta atcatcacca tccttcgtga ggaaaaaatt
    14101 gtttgtctgt ggagctttgt gccgccgccc aaccgacagc acggggatcg gcggcctccg
    14161 gcctccaacg cccgggcaga aactcgaatt agtagagctt cttggggagc atcatgctgc
    14221 gcaggcgctt gtagtgacgc ttgacggcag cagccttctt acgcttacgt tcggcggtgg
    14281 gcttttcgta gaactcgcgg gcgcggagtt cggtaaggag accagacttt tcgatggtgc
    14341 gcttgaagcg gcggagggca acttcaaacg gttcgttttc tttaacgcgg atagtaggca
    14401 ttagaaccta acctcagtaa acagtgtgat gacccggaaa tcaaatggaa agcctccggc
    14461 atctctggtt ccgggacaag agacggagga cgcaatcgaa caaagacggc ccctgagcgg
    14521 agccgtccga atgtcgagtc cgagattttg acataaaaca ctgggaaaat tcaaaccgaa
    14581 gcaccatttt tttgcacgtc caccgcaaaa aggctctaga attttcggca ctatgctagt
    14641 tctcggtatt gaatcctcct gcgacgagac gggagccgcc ctgatttccg acaaggacgg
    14701 cctcctcgca gacgccctct actcccagat cgacatgcac cgcgcctacg gcggcgtcgt
    14761 gcccgaactt gcgagccgcg accacatccg gcgcgccgtc gcactcgtcg acgaagtgct
    14821 cgtgaaggcg ggcaagaccc gcggagacat cgacgccgtc gccgtgacgg agggccccgg
    14881 acttgccggc gccctgctcg caggcgcggg cgttgcgcac gccatcggct gggcgctcga
    14941 tgtgcccgtg ctcggcgtgc accacctcga ggggcacctg cttgcagccc gtctttcgga
    15001 tcccgctccc aaattcccgt ttcttgccct tctcgtctcg ggcggccata cccagatcat
    15061 gaaggtggag aagttcggca gctacgagct tctcggccag actctcgacg acgctgcggg
    15121 cgaggccttc gacaagaccg cccagctgct cggcctgccc tacccgggcg gccccgccgt
    15181 gtccgccctt gccgaagagg gcgagcccgg cgccatagag cttgcccgtc cgatgatcca
    15241 cgcccccaat ctcgacatga gcttctctgg tctcaagacc tccgtgctca ccgccgtcca
    15301 gagtgctccc gacgcaggag acgaagtctt ccgccgtaat ctcgcccgcg gcttcgtgga
    15361 cgctcttgtc gacgtgctcg ccgccaagct cgtgcgcgcc atgaagcaga cggggcttcg
    15421 caacgtcgtc gtcgccggcg gcgtgtccgc caaccgccag ctcagacagc gcctcaccgc
    15481 cgaagtccgc cgccgtcgcg gcacgatctt ctatccgccc atgcggctct gcaccgacaa
    15541 cggcgccatg atcgccgccg caggcctcgc acgcctcgaa ggcatgacgg cagaggaact
    15601 cgaggcgctc aaggcgaagc gcagcttcgg cgtcagcccc cgctggcagc tctcaagcct
    15661 ctgacctccg ccccacggcc ctcagaactg aaaaaggatc cgatctttca gatacggatc
    15721 cttttttttg ttggtcgagt ctttgctgca ggtcccgatg aaggactact cctcgttcgc
    15781 gcagtcgacg gggatgccgc cgagcacgtc gacgagcacc ttgggatcga tgccgacctg
    15841 atagccccgt cgcccgccgt tgatgaagat atggtcaagc tcgagaatgg tcttctggac
    15901 gtagaccggc atcttctttc gggtgccgaa gggactcgtg ccgcccacca tgtagcctga
    15961 attgcgctgg gcctgctcgg gcttgcaggg cgcaacatgc ttggcgccga tctgtcgggc
    16021 gaggttcttc gtggagacct cgcagtcgcc gtgcatcagg atgaccagcg gtctggcgtg
    16081 ctcgtcctcc atgataagcg tcttgatcac gtgatgatga tcaatcccgc aggacgaggc
    16141 ggccagcgcc gtaccgccgt gctcgacgta ttcgtaggac ttttcctcaa agggaatgtc
    16201 gtgctgcctg agccactggg tggcgggcgt caggctctgg tgcttcactt tcgaactcat
    16261 tttctacttg cgtgcctttc ttatatagag gaagcggaaa gaaggattgt gcccgaagcg
    16321 ggcggatgcg gcgcgtgcgc cgtctcccgc ccatgcccga gcgcaagaac ggaatgcgac
    16381 tttaccttgc ggggctcagt cggcatcctc ctcgcgaagt cccttgggaa gcggaaagac
    16441 gacgttctcc tcgacgcctt cgagcgccac ggcatgctcg gcgttgaggt tggcctcaag
    16501 ataacgaagc acgccctgga cgagggattc aggggcggag gcacccgccg tgatgcccac
    16561 gcatgcaacg ccctcgaacc acttgggatc gatgtgctcg gccgtgtcga tcagataggc
    16621 gcgggccccc tcgcgttcgg ccacttcgcg cagacgattg gagttggagc ttgtggcgga
    16681 gccgatcacg agcacgatgt cgacgcccga gcgggcgaga agcttgacgg cctcctggcg
    16741 attcgtcgtg gcatagcaga tgtcctggcg cttgggttcc tgaatgaagg ggaagcggtg
    16801 cctgagcgcg tcgatcacgg cctgactgtc atcggcgctg atcgtcgtct gggtgacata
    16861 ggcaagcgag tcatcgttcg tgtagggcag cgctgctacg tcggcggtgt tctcgacaag
    16921 ctcgatgccg ccgtcgacct ggcccatcgt gccctcgacc tcggggtgac cggcgtgacc
    16981 gatcatcagc acggtgcgtc cttcggcgcg catgcgggca acctcgcgat gcaccttggt
    17041 gacaagcgga caggtggcgt cgaaaacgcg gaagccccgg cgctccgcct cttcagacac
    17101 cgccctcggc acgccgtggg ccgagaagac gaccgtcgca ccctcgggca cctcggcaag
    17161 attgtcgacg aagaccgcgc cgcgctcacg cagactgttg acgaccgtgc ggttgtgcac
    17221 gatctcgtgc ctgacataga tgggcgcgcc gtagatggca agcgcacgct caacgatcgc
    17281 gatggcgcgc gtgacgcccg cgcagaaacc gcgcggctgg gcaaggagaa ccttcatgac
    17341 tgtcatttcc tcgtgactga ctttgcttgt cggaggccgc agacgctgcc tgacggagcg
    17401 catatgcctg ccgcagtccc tcctaagagg tcgcaggcac gcgtgcggac gaaaaatttt
    17461 ccattaagtc taaagttttt tgggaaaatc cgcacacgca tcaaaaaaaa cgggtagaat
    17521 gcgctccacg ctcaaatctg cgcactgccg actccctgta ctgacacagt ggggcgtcag
    17581 cctgccatgc ggtgtcatgc actgcagaga caggtggcat gcgaacatga ggaggcgtat
    17641 aatttttctt ttatgcgctc gcgtggctgc ccgagaggga ccggcctgcg agatgtttta
    17701 ttttcatttc actggagtta gagatggcac gagtgtgtca agtcaccggt aaggcgccga
    17761 tggtcggcca tcacgtctcg cacgcgaaca acaagaccaa gcgtcgtttt atgcccaatc
    17821 tgcagtaccg ccgtttctgg gttgaaagcg aaaaccgttg ggttcgtctg cgtttgacga
    17881 cggctggcct tcgtacggtt gataagattg gtattgatgc ggttctggcg gacctccgcg
    17941 cccgcggcga aatctaatta agggagtttc accatggcga aaggcgctcg cgaaaagatc
    18001 aagcttgaat ccacggccgg taccggtcat ttttacacca ccacgaagaa caagaagacc
    18061 tcttccggca agatggaaat ctccaagttc gacccggtgg ctcgcaagca tgtgatctac
    18121 aaggaaacga agctcaagta atctgagcat tcgattcggc gaaaaagctc ggcaattcat
    18181 atcggccggg ctttttgctt gtccgaggac aggaaaaatc tccgaccgct gcccccgact
    18241 tcaggagaag tctggggcag cgtcattttt agggtagcgg cgcgcaaaac aaacgccgcc
    18301 ttccgaaagg agaggcggcg tgcaaaccgt tacgggcggt ctgtcagttc gcgtcagtca
    18361 cgctggagta cggcggcgaa gaagccgtca gtgctgttga catgcgggag catctggaag
    18421 tagctgccga agcgctccca ctgggaggcg tcgaactgaa tgccctgctg cgcgaatacg
    18481 tcggccgcgg gcacgagatg ccattcggga tgcgcttcga ggaaggcctc gacgacctcc
    18541 tggttttcgc gtctgagcat cgagcaggtg gcgtagacca tgcggccgcc cttcttcacg
    18601 agacgcgagg cgtgctcgag cacatccttc tggatcttgt tgatgcgatc aagttcggac
    18661 ggggcgagac gccacttcag gtcggggttt cgacggaagg tgccggtgcc cgtgcacggc
    18721 gcgtcgatga gcactcggtc gaacttgccc tgcaggcggc ggatgcgttc gtctcgctcg
    18781 ctgcggatcg cgatcggatg cacgttggag agacccgctc tgcgcatgcg gggcgtaagg
    18841 cccgcaaggc gcttttcgtt gacgtcaaag gcatagaggc tgcccgtcga gcgcatcagg
    18901 gcgccgatgg cgagcgtctt gccgcccgca cccgcgcaaa agtcgcagat catttcacgg
    18961 cggcgcgggg tgagcaggcg ggcgatgagc tgggaaccct cgtcctgaac gtccaccttg
    19021 ccttccttgt agatcggcca ctgggtgagg ccgggcttcg tcggcagacg aacgccgtcg
    19081 gggctgaagg gcgtcggata ggcctcgacg ccgttggcgg cgagctcctg aagaacctcc
    19141 tcacggctgg ccttcaggag attgacgcgc agatcgagcg gagcgccctc gagcatcgag
    19201 gcatagagct ccttcgtgtc gtcgtactgg gcttcgagga ggctgtagat ccactcgggc
    19261 agttcggcct gaatgtgggg gggcgccttc gagaaggctg cggcgagcac gttcttgagg
    19321 gggccttcct cgcggccgat ggaagcgggc gggagggact cgatgccgtg ctgccgtgca
    19381 agcgtcgcga gagcgacaag gcggggcgca cgggcgggag gcacgggatg catagcccag
    19441 gagagcgtgc cgagatggcg aagcgcatag tagatgccct cggcgataat gccgcggtcg
    19501 cgcatgccca gacgcgggtt gctgcggaag aagagcttca tcagcacgtc ggcgggaccg
    19561 tcaagcttca ggatgacgcc gagcgctcgg gtgagctcgt cgacgatcag gcccgtgatg
    19621 cggacttggc ccggctggag cgcagtcatg cgcttttcgg gcgtcttcgg gcccttcgcg
    19681 tagccgccca tggcgcgggc ctcctcgcgg cggcgcttgt cggcggcgcg ctggcgctcc
    19741 gtgcggcggg gcgactcagt gcgctcggtc ttgcggggct tctgcatcgc aaacgcgcga
    19801 cgcttcggga acttggtttc agccatatgt attcagcggt agttaaagca aaaaggaagc
    19861 gacccgaacc cctgcggggc ctgcgcttcc tccatgaggc cggcgaacgg accgtccgcc
    19921 ggcgcggtgt tgtcaaaaaa cggtcagcag ccgaagatcg tgagcgagcg ggcggtctcg
    19981 tcgttgcaga cggtgcggcc gtcctcacag cggacgcggc cttcgagaat ggcgcggacg
    20041 cacttcggat acagctggtg ctcgtagacg aggccgcggt gcgcaagcgc atcggcatcg
    20101 tcggacggca gaacgggcac gacggcctgg ccgatgatcg aaccgccgtc aagcacggcc
    20161 gtcacgaagt gaacggtcga gccgtgcacg cggcaacccg catcgatggc gcgctggtgc
    20221 gtattgaggc cggggaaaag cggcagcagg gcgggatgga tgttgaggat cttcccctcg
    20281 aacctggaga caaagccctc cgtcagaacg cgcatgaagc ccgccagaac gatcacggcc
    20341 ggatcatagc ggctgatgat gtcggcgagt tcggcctcga atgcctcgcg ggagtcgtag
    20401 gccttgtggt ccatggccac ggcctcgatg cccgcagcgc gcgcgatgtc aagaccagcc
    20461 gcctgagggc ggttggagat caccgccgca atgcgtgcat tgcaggcgga ctcccagttt
    20521 tcctcgcggc tcgttcgaag gatcgcctcg aagttgctgc cgcggccgga gataagaatt
    20581 acgatgttct gcatagtggc gtcattctaa accaaagacc gtgcacgctt cgggcggggc
    20641 agaagaaatt ttccgtataa tcggaacttt ccccaatcta gcaaaaggcc gttcaagtca
    20701 tgcacgtttt ccgcggtctg ccttctccgc tccagcgccg tccgtcggcg gtcgccatcg
    20761 gcaacttcga cggcgtccac ctcggtcatc aggcgcttct cgaatacgtg aaggaagccg
    20821 cgcaagcgcg cggactcgtt ccctccgtcg tcaccttcga gccccatccg aaggacttct
    20881 tcggccgcgg cggcgaagtc gcgcgcatct ccacgctcta cgacaaggtg aacgccatcc
    20941 ttggaaccgg catcgagcgc atctacatac tgccattcaa ctcgcgtctg gcctgcatgc
    21001 cggccgagac gttcgtgcgc gacgtccttc tcaagggact cgacgcctgc tggatgagcg
    21061 tcggcaagga tttccgcttc ggcggcgacc gcgcgggcag cgtcgagctt cttgaagagc
    21121 tctcccgcac actgccctac gagctctacg tctccccgac cttctatcac aaggaggccc
    21181 gcgtgagctc ctcgcgcatc cgcgaggccc tcgccgtagg cgacctctac gaggccgccc
    21241 tgatgctcgg tcgtccctac agcatgacgg gccgcgtcat ccacggcgcc gcgctcggcc
    21301 gcacgctggg cttccccacg ctcaacatgg cgccgattcc gccgggcagc cactcccgtc
    21361 ccgccgtcac gggcgtcttc gccgtccgca tccacggact cggacaggcc gttcagaacg
    21421 gcgtcgcctc gatcggcgtc aagcccacgg tcgcaaccga cgggcgctgg cttctcgaga
    21481 cgcacgtctt tgactggagc ggcagcgcct acggccgcat cgtcaaggtc gagttcgtcg
    21541 agcgcctgag gggagagaag aagttttccg gtcttgacga gctcaaggcc gccatcgagt
    21601 ccgacgccga tcatgcccgt cggattctcg ggtgcgtccg tcctcaggcg gacgtccccc
    21661 ttcaaggcat gaatccttaa cctttacctg aagagcccga gggggccagg agtactgacc
    21721 atggcaaaag aagccggatc ggccgccgac accaagaaat acccgctcaa ccttcccgac
    21781 acgccgttcc cgatgcgcgg caacctgccc aagcgcgaac cggaatggat cagggagtgg
    21841 gatgaaaagc acatctacga acgcatcatc gacgcccgcc gcgacgccaa gaagttcatc
    21901 cttcatgacg gtcctcccta cgccaacggc aacatccacg tcggtcacgc cgtcaacaag
    21961 atcctcaagg acatgatcct caaggaaaag acccttgagg gcttccaggc cccgtacgtt
    22021 ccgggctggg actgccacgg catgccgatc gaagtgcaga tcgagaagat gcacggcaag
    22081 aacctccccg tcgacaagat gcagagcctt gcccgtgcct acgccctcga gcagagcaag
    22141 ctccagctcg ccgacttcaa gcgcctcggc gttctcggcg actgggacaa cccctaccgc
    22201 acgatgaagt tcaagaacga ggccgaggaa atccgcgccc ttgccgccgt cgtgaaaaag
    22261 ggctatgtct accgcggcct caagcccgtg aactggtgct tcgactgcca gtccgccctg
    22321 gccgaagccg aagt
//
LOCUS       DBMDTI010000007        22191 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_794_length_22191_cov_2.996152, whole genome shotgun sequence.
ACCESSION   DBMDTI010000007 DBMDTI010000000
VERSION     DBMDTI010000007.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 22191)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22191)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22191
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_794_length_22191_cov_2.996152"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1081..1155)
                     /locus_tag="ACZPB6_01095"
     tRNA            complement(1081..1155)
                     /locus_tag="ACZPB6_01095"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1120..1122),aa:Gly,seq:tcc)
     gene            complement(1228..1848)
                     /locus_tag="ACZPB6_01100"
     CDS             complement(1228..1848)
                     /locus_tag="ACZPB6_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR1183108.1"
                     /translation="MTKHTTAECPYPKLEAPVPRAERENDPLTETSVAERVLLDGRFI
                     KVVEEDVVLPDGRPSRRFGLRHGGAAGMIALGADGTIVLERQWRHPLRRAFWEIPAGK
                     LDQGEAELDCAKRELVEECGIHAARWTRLGELNNAIGYSNERIVVFLAEDLSWGTQAL
                     DEGEHLEVVRVDLKEALDMCADGRITDVKTIVGLFWLERLLASRSA"
     gene            1972..3162
                     /locus_tag="ACZPB6_01105"
     CDS             1972..3162
                     /locus_tag="ACZPB6_01105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1183109.1"
                     /translation="MHAAVVGAGLTGLQTALSLVRKGADVTVIEAQRAPCQGASYCAG
                     AVLGDPAPAPIARPAGRLARLKALASNTSELVYGSGAAVRHPSFISAMTACREPARCE
                     ARDALAAELSEASTSMLRAEAQEHGFILQESAGTIIVSPEAGEASPATLEDVTAIEPS
                     LYAATDVGSFSFSRATTWSVSYYAKQLREHLAEAGVKILCSRKATGLISDNGRICGVA
                     ADEPVRTDAVVVACGTGALEILPEHAYGGVPLAPVTRSVLNVSLSGDACVMRHAVRTP
                     EGRIALPLDTFVRIMGRWHLGAQEHCPVDKEYKALWEMGVKLFPAATDWSQGRYLSHT
                     VLSSPDGLPVAGASGMPGLHLSIAGGIHGADFCTAVADVVSDGVLGRENPFSERLSWR
                     RFGG"
     gene            complement(3177..3731)
                     /locus_tag="ACZPB6_01110"
     CDS             complement(3177..3731)
                     /locus_tag="ACZPB6_01110"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HIR1183110.1"
                     /translation="MRCIRQDFNRKTAHALAQEYGCVYVEDLKVKNMTASVKGTVENP
                     GKNVKQKSGLNRAILRTGFYSLRQAIEWQLLKMGGLVIPVDPRGTSITCPHCQTRDKR
                     NRPTQAIFKCVNESCGFKANADVVGALNVLRKGRTGPSAHNKKHIAHVVSIGEQGFRP
                     EDSMPSMGAILKPRDRVAQGIPAL"
     gene            complement(3786..4337)
                     /locus_tag="ACZPB6_01115"
     CDS             complement(3786..4337)
                     /locus_tag="ACZPB6_01115"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HIR1183111.1"
                     /translation="MSQPLQQALRDLDRAIGDSFRPNSDPAHKGWPKFKAKDIGDGFR
                     IPQFAPEHIDDANGRVKLPKIGWIRYRRTRPIAFECADGMLVPGVVKQIHVRKDCGHW
                     FVIFTVEFEVKVPDMKALDVGIDVGVVHAVATNDGRFYDLDTAKIRSIEKRIAGLKRK
                     LSLNQESRKKLAKLGLAEPFDKK"
     gene            complement(4267..4560)
                     /locus_tag="ACZPB6_01120"
     CDS             complement(4267..4560)
                     /locus_tag="ACZPB6_01120"
                     /inference="COORDINATES: protein motif:HMM:NF023742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1183112.1"
                     /translation="MKITCAFQYELYVDETRRAALSRNAGCRRFVFNKALEIQNERKS
                     KGEKLLTYKELAHELVLWKKTTQDRLSSRGDVPTAPAGPARSRSRHRRQLPSQ"
     gene            4788..6263
                     /locus_tag="ACZPB6_01125"
     CDS             4788..6263
                     /locus_tag="ACZPB6_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HIR1183113.1"
                     /translation="MSLTILTADELQNLEMRAANQLGADTLMKRAGAAAAELIMKRLE
                     DAGVEQRRVTLLVGPGNNGGDALACACELREKGAVVNVVLPGGRRPTSALALAQLERW
                     TQAGGTTYDDPYMTEKADCVVDGLFGTGLAKPITGDYLDAVLWFNERQALKVSLDIPS
                     GLNPVTGHWTGSYPGCSADVTITFLCVKSGLYMCEGADAAGEIVLNELDVSVPLSPLS
                     VIGTDEFPRVLRPRVKNSHKGDYGSVAVIGGTDGMIGASILAARAALISGAGRVTLEC
                     RAEHAPHVDMVYPEIMFATKPVNLEDFDAIVLGCGLGTSAEAKARVIEALNCQKPLIL
                     DADALNIIAADIKLQDMVLARRAPTVLTPHPGEAARLLRRDTAGVTADRVAACRELAV
                     QTGAIVVLKGAGTVISMRSSRTWINPTGSPMLATGGSGDVLAGMIGAMFAQGYDMVES
                     VLAAVYFHGLSAEGLEAGFTAGEIAPNAMALVHDARVSYDL"
     gene            complement(6628..8136)
                     /locus_tag="ACZPB6_01130"
     CDS             complement(6628..8136)
                     /locus_tag="ACZPB6_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR1183114.1"
                     /translation="MDLVSNLLLGFQNAVTLQNLLYCFTGVSVGTLIGVLPGMGPVAT
                     VAMLLPITYALDPSAALIMLAGIYYGAQYGGSTTAVLVNIPGEAAAVVTCIDGHSMAK
                     KGRAGAALGIAAIGSFIAGTFATMLIAAFAPPLTELAFEFGPAEYFSLMVLGLVGAVV
                     LANGSLLKAVCMIFVGLILGLVGSDINSGALRYTFGVDELMDGIDFVAISMGIYGFAE
                     IMKNLEISQTRSLVPVKVESVLPTKEDLKVSAGPIARGTLLGSFLGILPGGGALLSSF
                     ASYTLEKKLAGERAEPAFGQGNIRGVAGPESANNAGAQTSFIPMLTLGIPSNAVMALM
                     IGAMMIHDITPGPQVMNTDPELFWGLIVSMWIGNLILIVLNLPMIGVWVQLLRVPYRW
                     LYPAILAFCCIGVYSINNNVFDVWMTIGFGALGYLFNKLGCESAPLILGFILGPMMEE
                     NMRRALLLSRGDMSCFLTSPLSAAFLALSVLLLLLVLAPAVRSGRETAFRED"
     gene            complement(8150..8608)
                     /locus_tag="ACZPB6_01135"
     CDS             complement(8150..8608)
                     /locus_tag="ACZPB6_01135"
                     /inference="COORDINATES: protein motif:HMM:NF018979.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR1183115.1"
                     /translation="MMIKNKKDFWAGIFFIAFGAGAAAVAQENALGTLSRMGPGFFPT
                     ALGVILALLGCCLSLRALLFSGYGDGSLEGVNWGVLGFILGSVAVFAVSLLELGVLLS
                     IALMIVISSLASGRFRAREVAVLTVFMCLLCWVIFIFGVGLQVPVLPAFL"
     gene            complement(8769..9773)
                     /locus_tag="ACZPB6_01140"
     CDS             complement(8769..9773)
                     /locus_tag="ACZPB6_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR1183116.1"
                     /translation="MIQRRTLIGAIGAVALSSASYFPMTACAAMPRGEGSDPIRIVVP
                     YPPGGPLDVSARTIAEAVVDTLGNVIVDNRPGAGGGVGMGYVKRRPGDGRTLVVGAVA
                     THAINPHLYKRLPYDAKKDFKAVTLIAHVPNVLVVTPEFSQKTGVKTVQNLVEYAKKN
                     PGKLNYASGGNGSAGHMAGEWLKQIKDIDMVHVPFAGAAAAQLSVLAGQTDLIFDNLA
                     SATANIRAGKLLPLAVTTNVRAKALPDVPTMQEAGVPDFDISTWYGLFVPASTPDDVV
                     AHLNKAITGALRSAPVQERLEKLGGVASPTTPEEFQELVTSESEKYARLVKLSGAGVD
                     "
     gene            10016..10798
                     /gene="ugpQ"
                     /locus_tag="ACZPB6_01145"
     CDS             10016..10798
                     /gene="ugpQ"
                     /locus_tag="ACZPB6_01145"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR1183117.1"
                     /translation="MQSVHPWPYARVSAHRGAGTMAPENTLEGFRAGLHHGFHAFETD
                     AMLAKDGVPVLMHDERFGRTIRDTRSVPELTSLEIRSLDAGSWFAPQYTGVPPAGFEQ
                     AVRWCRANRVWLNIEIKPAKGAELETGRVVGRLTGELYADLVREGGATQAGVVPDAPL
                     FSSFKPDALRGAMETAPNIPRGFLVDELPENWREVLEEMKCVALHCNQRRLTPEFARE
                     VKALGYWLFCYTVNDPRRARELFSWGVDGLCTDRLDLVRPSL"
     gene            complement(10920..11687)
                     /locus_tag="ACZPB6_01150"
     CDS             complement(10920..11687)
                     /locus_tag="ACZPB6_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1183118.1"
                     /translation="MNITTLRIGCSNCNLREVCMPLGLTLKDMETVEELVSARRRIRR
                     GEALYRAGDYFNNLYVVRLGFLKSTVMSSDGREQVTNFFMAGEMIGLDGISSNVYSCD
                     VVALEDTEVCVLPFDRVEQATTTIQTMGKHFQKLLSREIVRQHGVMLLLGSMHAEERL
                     AAFLLSLSQRFENRGYSRSEFVLRMTRAEIGSYLGLKLETVSRVLSRFSHDGLIEVNQ
                     KHVRIFDPEGLRAIVSAVDLDHSPSPVTASPMKIQKA"
     gene            11980..13380
                     /gene="hemN"
                     /locus_tag="ACZPB6_01155"
     CDS             11980..13380
                     /gene="hemN"
                     /locus_tag="ACZPB6_01155"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HIR1183119.1"
                     /translation="MTNPATSGVELPEMDLLRKFDVPAPRYTSYPTADRFNASFGAED
                     YRKALAGRADAKEPADLSLYVHVPFCNDVCFYCGCNKIVTRDHSRSAEYIEMIGREAD
                     LVKEAVTGSTELEQLHFGGGTPTFLTNDELTLMMETLTKRFPLAQGGEFSIEVDPRTC
                     DADKVKHLHDLGLNRMSLGVQDFNPDVQKAVNRIQPFEMTKATMEAARENGFESINMD
                     LIYGLPKQNRGTFEKTIDQVLELSPDRIALYHYAHLPNHFKPQRRILPADLPDTVEKV
                     NIMFDAIKRLTANGYRYIGMDHFAKETDELSVAQKEGSLQRNFQGYSTKAECDMIALG
                     VSSISKIGSAYACNPRELEDYYAAIREGRLATNRGYLLNADDEMRRYVIMTIMCNFEL
                     RKRDVEERFGIKFDETFAYELGKMKPYVKHELVELYDDRLVVSAKGKIFVRAVAMHFD
                     RYLRESTRAGGYSRIA"
     gene            13837..16464
                     /gene="alaS"
                     /locus_tag="ACZPB6_01160"
     CDS             13837..16464
                     /gene="alaS"
                     /locus_tag="ACZPB6_01160"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR1183120.1"
                     /translation="MKVADIRRTFLNFYESKGHTIVASSPVVPAHDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYTRATTSQKCIRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFK
                     HDAINFAWELLTKHFMLPAEKLWVTVYAEDDEAYNIWHEEVGVPAERIVRIGDNKGAR
                     YMSDNFWMMGDTGPCGPCTEIFYDHGDKIQGGPPGSPEEDGDRYIEIWNLVFMQFNRD
                     ERGVMHKLPKPSVDTGMGLERIAAVLQGVHNNYDIDLFKNLLASVKRVLEETSGEAVD
                     PESPSLKVIADHIRACTFSVADGIVPGNEGRAYVLRRICRRAIRHGYKLGARKPFFYK
                     LVDAVRAEMAEAYPEINNPRIEKVIEEEERRFFLTLSKGMEILDAAIAGSKDGVLDGE
                     VVFKLHDTYGFPADLTGDVCRERDVKIDFDGFDAAMARQREAARASAKFKMAAGLEYT
                     GADTVFTGYETLEENEATVLAVYKDGQAVELAEAGDDCIVVFDRTPFYAEQGGQVGDR
                     GHCRNDASLAGVLDTIRVKGKVTGHSCHVEEGRIAVGDKFRLTVDADRRHATQRNHSA
                     THLLQQALRKVLGEHVEQKGSLVSPTELRFDFSHSQAMTTEQIARVEDLVNEQILANT
                     ATKAEVLPLEEARKTGATMLFGEKYGDIVRVETIGNSVELCGGTHVVRTGDIGSFKIL
                     GEAGVAAGIRRIEAVTGMNALAEAQKNAALLGQAAFRFNVKACDLPSKIDDMMANVKA
                     LEKQLEQLKSQMASRMGDTLLEKAIEVKGIKVLAARMDGADAKALRETMDKVKDKLGT
                     AVAVLAAVSAEGRVQLAAGVSRDLTDRVKAGELVNSVAQKLGGKGGGKPDMAMAGAQS
                     AEGLDAALADVQAWVEAKL"
     gene            16474..16722
                     /locus_tag="ACZPB6_01165"
     CDS             16474..16722
                     /locus_tag="ACZPB6_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR1183121.1"
                     /translation="MTETLEVVDLCLPGLNCPMPVLRAKKALAKMAGGTLLRVVSTDR
                     HSLADLAEFCRQTGHELVSQNTDEAAGEYVTVIRRRED"
     gene            16922..16998
                     /locus_tag="ACZPB6_01170"
     tRNA            16922..16998
                     /locus_tag="ACZPB6_01170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16956..16958,aa:Arg,seq:acg)
     gene            17022..17098
                     /locus_tag="ACZPB6_01175"
     tRNA            17022..17098
                     /locus_tag="ACZPB6_01175"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17056..17058,aa:Arg,seq:acg)
     gene            complement(17421..17909)
                     /locus_tag="ACZPB6_01180"
     CDS             complement(17421..17909)
                     /locus_tag="ACZPB6_01180"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR1183122.1"
                     /translation="MKIALIVAAARNGVIGGGNKMLWRVPEDFAHFKRTTMGHPIVMG
                     RKTWESIGRPLPGRRNVVVTRNADYRAEGAEVVPSLEEAFRTLADTDTVFVLGGGEIY
                     RQALPSADVVWLTKIDADFEGDTTFPELPESEWTTEVLEMLEPTESRSFEVTFCRCER
                     RA"
     gene            complement(17920..18720)
                     /locus_tag="ACZPB6_01185"
     CDS             complement(17920..18720)
                     /locus_tag="ACZPB6_01185"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR1183123.1"
                     /translation="MPSNQYETLMRHVYEHGTHKSDRTGTGTRSVFGYQMRFNLAEGF
                     PLVTTKKLHLRSIIHELLWFLQGSSNIKYLHDNGVTIWDEWADENGDLGPVYGVQWRS
                     WPAPDGSHVDQIENLMQQLRTNPDSRRMIVCAWNPGLVDQMALPPCHCLFQFYVADGR
                     LSCQLYQRSCDIFLGVPFNIASYALLTHMVAQQAGLEVGDFVWTGGDCHLYDNHMEQT
                     ELQLSREPRPYPQLVIRRKPDSIFDYRFEDFEITGYDPWPHIKAPVAV"
     gene            complement(18876..20705)
                     /locus_tag="ACZPB6_01190"
     CDS             complement(18876..20705)
                     /locus_tag="ACZPB6_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR1183124.1"
                     /translation="METGTGVNALTIVFAALCIFAIAYRFYGLFIATKVLSLNEARKT
                     PAVKYSDGQDYVPTNKYVLFGHHFAAIAAAGPLLGPVLAAQFGYMPGLLWILIGCVLA
                     GGVHDMVVLFCSVRHRGRSLAYIATQEIDRTTGFVASWAVLAILILTLAGLSIAVVNA
                     MHNSLWSTYTVAATIPIAILMGLYMQIWRKGDVVGATVIGIVLLFLCILSGPWVAAHP
                     EYFGWLDIDRKAMSILIPIYGFFASVLPVWLLLLPRDYLSTFLKIGTIGALAIGIAFV
                     MPNFQMPAFTDFIHGGGPIVGGPVIPFIFITIACGALSGFHATIGTGTTPKMVGNERD
                     VLFVGYGAMLMEGFVAIMALVAACVLVPADYFAINAPAEKFQALGMAVQELPRLEAEV
                     QESLMARPGGSVSLAVGMAHIFSKIPFMEHLMAYWYHFAIMFEAVFILTAVDAGTRVG
                     RFFLQEMIGKVIPKFADKEWVPGVIITSVLFTGSWGYLVYSGDIGSIWPLFGICNQLL
                     ASVTLLIGTTVILRMNKAKYAWITGVPGLFMTVITFWAGIWLIVNQYLPGGKILLATL
                     SVIVMLLMLFVIYGTLRRWFALLAIHTTTKDEYGEDVKVVVQE"
     gene            complement(20904..21470)
                     /locus_tag="ACZPB6_01195"
     CDS             complement(20904..21470)
                     /locus_tag="ACZPB6_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183125.1"
                     /translation="MPQQARDAEFAPHAAIDLRDQNSMFLALEELGIACMQDAWIRSN
                     DPGHKDAAAVHEQAEEFLADILMLLSTGGDESRIVQNGWAGAELAKHLRASLRQELAA
                     ETAAQGLEGGTDAMSDEVVIRLALSCYLKDLELLTARDAHEKMLGRKIEPKEYIDFMI
                     GWSGVFSGAKPSLELPMAFMIFKGAQKH"
     gene            complement(21549..22001)
                     /locus_tag="ACZPB6_01200"
     CDS             complement(21549..22001)
                     /locus_tag="ACZPB6_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HIR1183126.1"
                     /translation="MGIKICNLCCGHGHVFEGWFRSDEELEADLKEGRIECPVCGDPC
                     VAKRPSAPNFVRVQGTTRTDVAADVAARGWERAREMQADAMKVLREAAAMAEDVGERF
                     PETVRDIHDGRAERRLVKGTCSGEEAQALHEEGIPVMPLPDAVSRRLN"
BASE COUNT         4303 a         7173 c         6775 g         3940 t
ORIGIN      
        1 ttatggcttc cgagacgatc tgtacttttt cttaaaagtc aaggccgcgt tccacggact
       61 tccgagatga acctagaaaa tgtctggaag atgtctaaca gatgtctaac tctgtcccat
      121 catgctaacc gaaaacgggc gaaaacccac ggctcaacgg ggaaatgtct aagtgatgtc
      181 taggggatgt ctaggagatg tctaacgggg ttcgacagac cccttctccg ccagtttctg
      241 aacagggctc tggactaagt tccgagccct gttttttttc atgaaaaaga tgagagcaaa
      301 gcacgcaaaa acccttgcgg cccgaagtcg catcatgaaa gattcgcagg aaccaagccc
      361 tcatcaccac accgagatct gcgtcgcacc cgtatctctt catgagctga tctgattgtt
      421 tggcgatgca cctgctcctt agttttcatc atagagggtg atgaacggtt tgtcatcatt
      481 tccatttttt acgcgtgtca cgttcgcgaa tgggcggctc tccaccatcc accaatttga
      541 tcctgcagct gccgcaacat ggcgacagct tttcaattgc caccacggca cgggcgccat
      601 tcgtcaggca gccaaacctc gtcggtgaat cgggagagtt tcgatatagc cgagccttgt
      661 tccggggcgt aggtttgttt gtcaagacct gcgtacaaca ggttaagcca agccgccacg
      721 acgcgtcgct cccaaagttc gtgcttgacg aattgccccg tcagcagata gtgctgcgta
      781 ggcttgagct tctttgtcga tacgactttc accaacaagt cgtattcgtc aagcggaacc
      841 gcacacgagt atcgcggaaa gcttttgtcc tttccctgcc cttcggcatg gctcgtgcgg
      901 ctttccactc aggtgtggca cgcatctgca gaacacgatc caatcctccg cccatgacgg
      961 cgttctgagg ctgactgcga agctcaaact cttccgcaac cgacggcgct cttgatctcc
     1021 cgacaagagc gcagaaaaca aaaaagcccc gcgggccgac tgcctgcgag gcgattttca
     1081 tggagcgggt gaagggagtc gaaccctcgt ctccagcttg gaaggctggg gtaatagccg
     1141 ttatacgaca cccgcttcga gagttttgga atcttaccac agagcccgtc gaattgccaa
     1201 acacccgagc cctgcggacc gtgcgcctca ggcgctgcgc gaggcgagaa ggcgttcgag
     1261 ccagaagagg ccgacgatgg tcttgacgtc cgtgatgcgg ccgtcggcgc acatgtcgag
     1321 cgcctccttc agatcgacgc ggacaacctc gagatgctcg ccctcgtcaa gggcctgcgt
     1381 gccccaggag aggtcctcgg ccagaaacac gacgatgcgt tcattggaat aaccgatcgc
     1441 gttgttgagt tcgccgagtc gcgtccagcg cgctgcgtga atgccgcact cctcgacgag
     1501 ctcgcgcttc gcgcagtcga gctcggcctc gccctgatcg agcttgcccg caggaatctc
     1561 ccagaaggcc ctgcgcagcg gatggcgcca ctggcgttcg aggacgatcg tgccgtcagc
     1621 ccccagggcg atcatgccgg ccgcgccgcc gtggcgcagg ccgaagcggc ggctcggacg
     1681 tccgtcggga aggacgacgt cctcctcgac caccttgatg aagcggccgt cgaggagcac
     1741 gcgctcggcg acggacgttt cggtgagcgg atcgttctct cgctcggcgc ggggaacggg
     1801 agcctcaagc ttcggatagg ggcactcggc ggtcgtatgt ttggtcatgg cggatgttcc
     1861 ggaaaaaagc ggttccttca gtatatgcca gccgtacgtg cgggggaggc ccatgccggc
     1921 ggactgggct acaatgaccg attccttttt tcccaaaaaa aataccagta catgcacgcc
     1981 gcagtcgttg gagcgggcct cacgggcctg cagaccgccc tgtccctcgt cagaaaaggt
     2041 gccgacgtca ccgtcatcga agcccagcgc gcgccctgcc agggcgcgag ctactgtgcg
     2101 ggcgccgttc tcggcgaccc cgcacccgcg ccgatcgcac gtccggcagg acgtcttgcg
     2161 cgcctcaagg cgctcgcctc gaacacctcg gaactcgtct acggcagcgg cgcggccgtt
     2221 cgccacccga gcttcatctc cgccatgacc gcatgccgcg agcccgcgcg atgcgaggcg
     2281 agggacgcgc tcgccgcgga gctctccgag gcctcgacgt cgatgctcag ggccgaggcg
     2341 caggagcacg gcttcattct tcaggaaagc gcgggcacga tcatcgtctc gcccgaggcc
     2401 ggcgaagcgt cccccgcgac gcttgaggac gtcaccgcca tcgagccctc gctctatgcg
     2461 gccacggacg tcgggagctt ctcattctcg cgcgcgacca cctggtccgt gagctactac
     2521 gccaagcagc tcagggaaca cctcgccgaa gcgggagtga agatcctctg cagcaggaag
     2581 gcgacgggcc tcatctccga caacggccgc atctgcggcg tggcggcgga cgagcccgtc
     2641 aggaccgacg ccgtcgtcgt cgcctgcggc acgggcgccc ttgaaattct tcccgaacat
     2701 gcctacggcg gcgtgccgct tgcgcccgtc acccgctccg tgctcaacgt gagcctctcg
     2761 ggcgacgcct gcgtgatgcg ccatgccgtg aggacgcccg agggacgcat cgcactgccg
     2821 ctcgacacgt tcgtgcgcat catgggccgc tggcatctcg gcgcccagga gcactgcccc
     2881 gtcgacaagg agtacaaggc gctctgggaa atgggcgtga agctcttccc cgccgccacc
     2941 gactggtctc aggggcgcta tctctcgcac accgtgctct cgagccccga cggcctgccc
     3001 gtcgcaggcg cctccggtat gccggggctg cacctgagca ttgcgggcgg catccacggc
     3061 gccgacttct gcacggccgt ggcggacgtc gtctccgacg gcgtcctcgg acgcgagaac
     3121 cccttctccg aacgcctctc ctggcggcga ttcggcggtt gacttcctcc tccgtcctaa
     3181 agggcgggga ttccttgggc cacgcgatcg cgtggcttga ggatggctcc cattgatggc
     3241 atcgagtcct ccggccgaaa gccttgctcc ccaatactca ctacatgagc gatgtgcttt
     3301 ttgttgtgtg cactcgggcc tgtccggccc ttcctcaaca cgttcagggc gcccaccacg
     3361 tcggcattcg ctttgaagcc gcaactttcg ttgacgcact tgaaaatcgc ctgagtcggg
     3421 cggttgcgtt tgtccctggt ctgacagtgc gggcaggtga tgctcgtccc tctcggatcg
     3481 acgggaatca ccaaaccacc cattttcaga agttgccatt cgattgcttg gcgaagactg
     3541 tagaaccccg tgcgcaggat ggcgcggttg agcccgcttt tctgcttcac atttttgccg
     3601 gggttctcaa cggtgccctt gacggacgcc gtcatgttct tcaccttcag gtcctcgacg
     3661 tacacgcagc catactcctg cgcgagcgca tgcgccgtct tcctgttgaa atcctgtcga
     3721 atgcaacgaa tctttcgata taacttttga agcttctcct tgagccgacg ccgcttgtaa
     3781 ctcggctact tcttgtcaaa aggctccgcc agtcccaact tcgcgagttt ctttcgcgac
     3841 tcctggttga gcgaaagctt gcgtttcaag cctgcaatgc gtttctcgat cgagcgaatc
     3901 ttcgccgtgt cgagatcgta gaatcgtccg tcgttcgtcg cgacggcatg cacgacgccg
     3961 acgtcgattc caacatcgag cgccttcatg tcggggacct tgacttcgaa ctcaaccgtg
     4021 aagatcacga accagtggcc gcagtccttc ctgacgtgaa tctgcttcac gacaccggga
     4081 acgagcatcc cgtccgcaca ctcgaacgcg atgggacgcg ttctgcggta tcgaatccaa
     4141 ccgatctttg ggagcttgac tcgcccgttg gcatcgtcga tgtgttcagg agcaaattgc
     4201 ggaatccgaa agccgtcacc gatgtctttc gccttgaact tcggccaccc tttgtgtgcc
     4261 ggatcgctat tgggacggaa gctgtcgccg atggcgcgat cgagatcgcg cagggcctgc
     4321 tggagcggtt gggacatcgc ctctcgaaga aaggcggtct tgggttgttt ttttccagag
     4381 caccaactcg tgagcgagct ccttgtaagt gagaagcttt tcacctttgg acttgcgctc
     4441 gttttggatc tccaacgcct tgttgaagac aaacctgcgg caaccggcgt ttcgactcaa
     4501 ggccgcacgt cgggtctcgt caacgtagag ttcgtattga aaggcgcagg tgatcttcat
     4561 acgaacatca tggcacactt ccttcaggaa tgcgtgtttt tcaaccaaat gcccattggg
     4621 gccttatatc ctccccatga atggagaggt tttacggccc gttggataag gcggcctttg
     4681 agaagcctcc tgtgccgcgt gcgggcgccg cggccattcg tcaacatcaa taatgtgcga
     4741 gtacaataac ccgcacattt tcctttcagg acccaaggaa cccgatcatg agccttacca
     4801 ttctcaccgc agacgaactg cagaatctgg aaatgcgcgc cgccaaccag ctcggcgccg
     4861 atacgctgat gaagcgcgcc ggcgcggccg ccgccgaact catcatgaag cgtctcgagg
     4921 atgccggcgt cgaacagcgc cgagtcaccc tgctcgtcgg tccgggcaac aacggcggcg
     4981 acgcccttgc ctgcgcctgc gagctccgtg agaagggcgc cgtcgtgaac gtggtgctcc
     5041 cgggcggccg ccgcccgacc tccgccctcg cactcgccca gctcgaacgc tggacgcagg
     5101 ccggcggcac gacttatgac gatccgtaca tgacggaaaa ggccgactgc gtcgtcgacg
     5161 gtctcttcgg cacgggcctt gccaagccga tcacgggtga ctacctcgac gccgtcctct
     5221 ggttcaacga acgccaggcc ctcaaggtct cgctcgacat tccgtccggt ctcaaccccg
     5281 tcacgggtca ctggaccggc agctatcccg gctgcagcgc cgacgtgacg atcacgttcc
     5341 tctgcgtcaa atccggcctc tacatgtgcg aaggcgccga cgccgccggc gagatcgtcc
     5401 tcaacgagct cgacgtctcc gttccgctct ccccgctctc cgtgatcggc accgacgaat
     5461 tcccgcgcgt tcttcgtccc cgcgtcaaga actcccacaa gggcgactac ggcagcgtgg
     5521 ccgtcatcgg cggcaccgac ggcatgatcg gcgcctcgat cctcgcagcc cgcgccgccc
     5581 tgatctcggg cgccggccgc gtcacgctcg aatgccgcgc cgagcatgca ccgcacgtcg
     5641 acatggtcta tccggaaatc atgttcgcga ccaagcccgt caatctcgag gacttcgacg
     5701 cgatcgtcct cggctgcggt ctgggcacct ccgccgaagc gaaggcccgc gtcatcgagg
     5761 ccctcaactg ccagaagccg ctcatccttg acgccgacgc gctcaacatc atcgccgccg
     5821 acatcaagct tcaggacatg gtgctcgccc gccgcgcccc gaccgtcctc acgccgcacc
     5881 cgggcgaagc cgcccgcctg ctgcgccgcg acaccgccgg cgtcaccgcc gaccgcgtcg
     5941 ccgcctgccg cgagctcgcc gtccagactg gcgccatcgt cgtcctcaaa ggcgcgggca
     6001 ccgtcatctc gatgcgcagc tcccgcacct ggatcaaccc gaccggcagc cccatgctcg
     6061 ccacgggcgg ctcgggcgac gtcctcgccg gcatgatcgg cgccatgttc gcccagggct
     6121 acgacatggt cgagtccgtg ctcgccgccg tctacttcca cggcctctcc gccgagggcc
     6181 tcgaagcggg cttcaccgca ggcgaaatcg ctccgaacgc catggccctc gtgcacgacg
     6241 ccagggtgag ctacgacctc tgacgatcgg acggcttgtt tcacgaatcc gcgccgcgca
     6301 acaaagtttt gcgcagccgg acatcgaaaa tacctgatgc ccaacaattc gagcaaattt
     6361 caaccgaaaa cgggaattac attctcttac ggacaatgac tcaaggaaaa acatcccgga
     6421 gccctctgcc tgtaatcttc tactcaacga agcacgcaag tgctgagtct cctgaatcgg
     6481 ttggataccg ctaacaatga gggtttgccc cgcgttccga aaggacgcgg ggattttttt
     6541 cgccctgaaa acggcagggg gccggcggat cattccgccg gcccccttgc ctcaggttcg
     6601 tcgtgctcgt tcatgtctcg accgaagcta gtcctccctg aaggccgtct cgcggcccga
     6661 gcgcacggcc ggggcgagca cgagaagcag cagaaggacg ctcagcgcaa ggaacgccgc
     6721 cgagagcggg ctcgtgagga agcagctcat gtcgcccctc gagagcagaa gcgcgcggcg
     6781 catgttctcc tccatcatgg gaccgaggat gaagcccaga atgaggggcg cgctttcgca
     6841 gccgagcttg ttgaagagat agcccagcgc accgaagccg atcgtcatcc acacgtcgaa
     6901 gacgttgttg ttgatcgagt agacgccgat gcagcagaag gcgaggatcg cgggatagag
     6961 ccagcggtac ggtacgcgca ggagctgcac ccagacgccg atcatcggca ggttgaggac
     7021 gatcaggatg aggttgccga tccacataga gacgatgagg ccccagaaga gttcggggtc
     7081 ggtgttcatc acctgggggc caggcgtaat gtcatggatc atcatggcgc cgatcatgag
     7141 cgccatgacg gcgttggacg ggataccgag cgtgagcatc gggatgaagc tcgtctgggc
     7201 gcccgcgttg ttggcggact cggggcccgc cacgccgcgg atgttgccct gtccgaaggc
     7261 gggttctgcg cgttcgcccg cgagcttctt ctcgagggtg taggacgcaa aggacgagag
     7321 aagcgcgccg ccgcccggca ggatgccgag gaaggatccg agcagcgttc ctcgggcgat
     7381 cgggcccgcc gacaccttga ggtcctcctt cgtcgggagc acgctctcga ccttgacggg
     7441 cacgaggctg cgcgtctggc tgatctcgag gttcttcatg atctccgcga agccgtagat
     7501 gcccatcgag atcgccacga agtcgatgcc gtccatcagc tcgtcgacgc cgaacgtgta
     7561 gcgcagagcg cccgaattga tgtccgaacc cacgaggccg aggatgaggc ccacgaagat
     7621 catgcagacg gccttgagaa gcgagccgtt ggcgagcacc acggcgccca cgaggccgag
     7681 caccatcagg ctgaagtact cggcggggcc gaactcgaag gcgagctcgg tcaggggcgg
     7741 cgcgaaggcc gcgatcagca tcgtggcgaa ggtgcccgcg atgaagctgc ctatggccgc
     7801 aatgccgagc gccgcgcccg cgcgaccctt cttggccatg ctgtggccgt cgatgcaggt
     7861 caccactgcg gcggcctcgc ccggaatgtt gacgaggacg gccgtcgtgg agccgccgta
     7921 ctgggcgccg tagtagatgc ccgcaagcat gatgagggcg gccgacgggt cgagcgcgta
     7981 cgtgatcggc aggagcatcg ccacggtggc aacgggcccc atgccgggca gcacgccgat
     8041 gagcgtgccc acggagacgc ccgtgaagca gtagagaaga ttctgaagcg tgacggcgtt
     8101 ctgaaagccg agcagcaggt tggagacgag atccatgtgc gcacctccct cagaggaagg
     8161 ccggcagaac cggcacctga aggcccacgc cgaagatgaa gatcacccag cagagaaggc
     8221 acatgaagac cgtgagcacc gccacctccc ttgcacggaa gcgtccgctg gcaagcgacg
     8281 agatcacgat catgagggcg atggagagca ggacgccgag ctcgaggagc gagacggcga
     8341 agaccgcgac gctgccgagg atgaatccga gtacgcccca gttgacgcct tcgagggagc
     8401 cgtcgccata accggagaag agcagagcgc gaaggctcag gcagcagccg agcagggcca
     8461 gaatgacgcc aagggccgtc gggaagaagc cgggccccat gcgcgagagc gtgccgagcg
     8521 cattctcctg ggcaacggcc gcggcgccgg cgccgaaagc gatgaagaat atgcctgccc
     8581 agaagtcctt tttgtttttg atcatcattt caaggggtct gcgtcagccg cacggccgtc
     8641 cgggccgcgc ggctgcgccg tgcgttagtc attgttttcg ttgaacagcg agccttgatt
     8701 ctagcgaatg agaaaaaagg cctcgattcc ttgggattcg aggccttcca ttcgagctgc
     8761 gccctgcgtc agtcgacgcc tgcgccggaa agcttgacaa gccttgcgta cttctcgctt
     8821 tcgcttgtca cgagctcctg gaactcttcg ggcgttgtcg gagaggcgac gccgccgagc
     8881 ttttcaagcc tttcctgaac gggcgcggac ctgagggctc ccgtgatcgc cttgttgagg
     8941 tgcgcgacca cgtcgtcggg cgtggaggcg ggcacgaaga ggccgtacca cgtggagatg
     9001 tcgaagtcgg ggacgcccgc ctcctgcatc gtcgggacgt cgggcagcgc ctttgcgcgc
     9061 acattcgtcg tgacggcgag cggcaggagc ttgcccgcgc ggatgttggc ggtggcgctc
     9121 gcgaggttgt cgaagatcag gtcggtctga cccgcgagca cggagagctg cgcggcggcg
     9181 gcacccgcga acgggacgtg gaccatgtcg atgtccttga tctgcttgag ccattcgccg
     9241 gccatgtggc ccgcgctgcc gttgccgccg gaggcgtagt tgagcttgcc ggggttcttc
     9301 ttcgcgtatt cgacgagatt ctggaccgtc ttgacgcccg tcttctgcga gaactcgggc
     9361 gtgacgacga ggacgttggg cacgtgggcg atgagcgtca cggccttgaa gtccttcttc
     9421 gcgtcatagg gcaggcgctt gtagagatgc gggttgatcg catgcgtggc gaccgcgccc
     9481 acgacaagcg tgcggccgtc gccgggacga cgcttgacgt agcccatgcc gacgccgccg
     9541 cccgcaccgg gacggttgtc gacgatgacg ttgccgagcg tgtcgacgac ggcttccgcg
     9601 atcgtgcggg cggagacgtc gagaggccct cccggcgggt acggcacgac gatgcggatc
     9661 ggatccgaac cttcaccccg aggcatggcc gcacaggctg tcatcgggaa ataggaggca
     9721 ctagagagtg ccaccgcgcc gatggcaccg ataagagtgc gacgttgaat catattgatt
     9781 tctcctctga gagatgaagt tgttccaagc atatacgcta tggacgggaa atggcaaaac
     9841 taggcacttt gttttaggcg cacccgcaac aacctctgtc acaggcgttt cgagcgcata
     9901 ttagggtttt cgccctaggt attcaccact atgaaaatcg ttggcgagcc tacaattgcc
     9961 ttgttgaaaa aggcggcgga tcccgaccgt cgccccgaca tgaggacaca tcatcatgca
    10021 atccgttcat ccctggccct atgcccgcgt ctccgcccac cgcggtgcgg gcacgatggc
    10081 gcctgaaaac accctcgagg gtttccgcgc cggtcttcac cacggcttcc acgccttcga
    10141 gacggacgcc atgctcgcca aggacggcgt tcccgtcctg atgcacgacg agcgcttcgg
    10201 ccgcacgatc cgcgacacac gctccgtgcc cgagctcacg agcctcgaga tcaggtcgct
    10261 tgacgcgggc agctggtttg cgccgcagta cacgggcgta cctcccgcag gcttcgagca
    10321 ggccgtccgc tggtgccgcg ccaaccgcgt ctggctcaac atcgagatca agcccgccaa
    10381 gggcgccgag ctcgagacgg gccgcgtcgt cggccgcctg acgggcgagc tctatgccga
    10441 cctcgtgcgc gagggcggcg ccactcaggc gggcgtcgtg cccgatgctc cgctcttctc
    10501 gtccttcaag cccgacgccc ttcgcggcgc gatggagacc gccccgaaca ttccgcgcgg
    10561 cttcctcgtc gacgagctgc ccgaaaactg gcgcgaggtg ctcgaggaga tgaagtgcgt
    10621 ggcgcttcac tgcaaccagc gccgcctgac gcctgaattc gcacgcgagg tgaaggccct
    10681 gggctactgg ctcttctgct acacggtgaa cgatccgcgc cgcgcccgcg agctcttctc
    10741 gtggggcgtc gacggcctgt gcaccgacag gctcgacctg gtgcgcccgt cgctctgagg
    10801 cacgcacccg acgtctgaag tctcaggaag ggcctgaatg agggcccgaa tgaagactca
    10861 aaagcccgcc atttccatca ggaaaggcgg gcttcttcat gtcctgcagg gcgtggccat
    10921 caggccttct ggatcttcat gggcgaggcc gtgacgggcg agggcgagtg gtcgaggtcg
    10981 accgccgaga cgatggcgcg caggccctcg gggtcgaaga tgcggacgtg cttctggttg
    11041 acctcgatga ggccgtcatg ggagaagcgc gagagcacgc ggcttacggt ctcgagcttg
    11101 aggccgaggt aggagccgat ttcggcgcgc gtcatgcgca gcacgaactc gctgcgggag
    11161 tagccgcggt tttcgaagcg ctgggagagc gacaggagga aggcggcaag acgctcctcg
    11221 gcgtgcatgg agccgagcag cagcatcacg ccgtgctggc gcacgatttc gcgagagagc
    11281 agcttctgga agtgcttgcc catcgtctgg atcgtggtcg tcgcctgttc gacgcggtcg
    11341 aaggggagga cgcagacctc cgtgtcctcg agcgcgacga cgtcgcagga gtagacgttg
    11401 ctcgaaatgc catcaaggcc gatcatctcg ccggccatga agaagttggt gacctgctcg
    11461 cgaccgtcgc tgctcatgac ggtgctcttc aggaagccga ggcgaaccac atagagattg
    11521 ttgaagtagt cgccggcacg gtagagcgcc tcgccgcgac ggatgcggcg gcgggcggag
    11581 acaagctcct ccaccgtctc catatccttg agcgtaaggc cgagcggcat gcagacctct
    11641 ctcaggttgc agttcgaaca gccaatgcgc agtgtcgtga tattcatctt gcccccgttg
    11701 ctgaggaagg cccgaagtca ggccactccc ctcgatgaaa ccaagaccgc cggccgccca
    11761 gtccgcggcc gcgttctcgc gttcaataat ctagaaataa tctgccgcgt ctagaatgcc
    11821 cacatgcatc ctgaagcacg gaccctttct gtcgtcgaag tgccgtcaca cagcacttcc
    11881 gacgcgagcg cttgttgacg tgagtcaaag aaaagcgatc tacggcctca ataatatcag
    11941 gaagtttaac ttaagtcaca gaccaaggaa aatcctcaga tgacgaatcc tgcaaccagc
    12001 ggcgttgaac tgcccgaaat ggacctcctg cgcaaattcg acgtgcccgc cccccgctac
    12061 acgtcgtatc cgaccgccga ccgattcaac gcgagcttcg gtgccgaaga ctaccgcaag
    12121 gccctcgccg gccgcgctga cgcgaaggag cccgccgacc tctcgctcta cgtgcacgtt
    12181 cccttctgca acgacgtctg cttctactgc ggctgcaaca agatcgtcac gcgcgaccac
    12241 tcccgcagcg ccgagtacat cgagatgatc ggccgcgaag ccgacctcgt gaaagaggcc
    12301 gtcacgggct ccaccgagct cgagcagctc cacttcggcg gcggcacgcc gaccttcctc
    12361 acgaacgacg agctcacgct catgatggag acgctcacga agcgcttccc gctcgcccag
    12421 ggcggcgagt tctcgatcga ggttgacccg cgcacctgcg acgccgacaa ggtcaagcac
    12481 ctgcacgatc tgggcctcaa ccgcatgtcc ctcggcgtcc aggacttcaa ccccgacgtc
    12541 cagaaggccg tgaaccgcat ccagcccttc gagatgacga aggccacgat ggaggccgcc
    12601 cgcgaaaacg gcttcgagtc catcaacatg gacctcatct acggcctgcc caagcagaac
    12661 cgcggcacgt tcgagaagac gatcgaccag gtgctcgagc tctcccccga ccgcatcgcg
    12721 ctctatcact acgcccacct gccgaaccac ttcaagccgc agcgccgcat cctgcccgcc
    12781 gaccttcccg atacggtcga aaaggtcaac atcatgttcg acgccatcaa gcgcctgacg
    12841 gccaacggct accgctacat cggcatggac cacttcgcca aggagaccga cgaactctcc
    12901 gtagcccaga aggaaggctc cctgcagcgc aacttccagg gctactcgac aaaggccgaa
    12961 tgcgacatga tcgccctcgg cgtctcgagc atctccaaga tcggcagcgc ctacgcctgc
    13021 aacccgcgcg agcttgagga ctactacgcc gccatccgcg agggccgcct cgccacgaac
    13081 cgcggctacc tgctcaacgc cgacgacgaa atgcgccgct acgtgatcat gacgatcatg
    13141 tgcaacttcg agctccgcaa gcgcgacgtc gaggaacgct tcggcatcaa gttcgacgaa
    13201 acgttcgcct acgagctcgg caagatgaag ccctacgtga agcacgagct cgtcgagctc
    13261 tacgacgacc gcctcgtcgt ctccgccaag ggcaagatct tcgtgcgcgc cgtcgcgatg
    13321 cacttcgacc gctacctgcg cgaatccacc cgcgcgggcg gctactcccg catcgcctaa
    13381 gcggcccggc acggcgcgtc ccgccgggcg tctcaggacg cccggagacg acgcccgcac
    13441 cctccgacgc ccgcctcccg tgaggcgggc gtctcctttt tgcacgatcc ggcccccgtt
    13501 tcgaccctca gggcatgaaa atccgcattc cgacagggag ttgcagggcc gaacccatgc
    13561 gatgggggta aaatatgagt tttcgtcccg agtcttcacg aaggctcccg acacaccgtt
    13621 catgccgcgg ccggcactgc cggccgtcag ccagagcggg ccgcctcgga gctcgccgtc
    13681 gccgccacag gcagaccgac ctccgttccc gccgccggcc ggagcatccc gcctccggat
    13741 gcagctgcac ggcaccgaat tgacaaaaac ctataggaga tggaacggcc ctgaccaggc
    13801 gcacgccgcc tgacagggag tcccgaaaac ccatttatga aagtcgcaga cattcgccgc
    13861 acctttctga acttctacga aagcaagggc cacacgatcg tcgcctcgag cccggtcgtc
    13921 cccgcccatg acccgacgct tctcttcacg aacgccggca tgaaccagtt caaggacgtg
    13981 ttcctcggct tcgacaagcg cccgtacacc cgcgccacga cgtcccagaa gtgcatccgc
    14041 gccggcggca agcacaacga ccttgacaac gtcggctaca ccgcccgcca ccacacgttc
    14101 ttcgagatgc tcggcaactt ctcgttcggc gactacttca agcatgacgc catcaacttc
    14161 gcctgggagc tcctgacgaa gcacttcatg ctgcccgccg aaaagctctg ggtcacggtc
    14221 tatgccgagg acgacgaggc ctacaacatc tggcacgagg aagtcggcgt tcccgccgag
    14281 cgcatcgtcc gcatcggcga caacaagggc gcccgctaca tgtccgacaa cttctggatg
    14341 atgggcgaca cggggccgtg tggtccctgc accgaaatct tctacgacca cggcgacaag
    14401 atccagggcg gccctccggg gagccccgag gaagacggcg accgctacat cgagatctgg
    14461 aacctcgttt tcatgcagtt caaccgcgac gagcgcggcg tcatgcacaa gcttccgaag
    14521 ccctccgtcg acacgggcat gggtcttgag cgcattgccg ccgttctcca gggcgtgcac
    14581 aacaactacg acatcgacct cttcaagaac ctgctcgcca gcgtcaagcg cgtccttgag
    14641 gagacgagcg gcgaagccgt cgatccggaa tccccgtccc tcaaggtgat cgctgaccac
    14701 atccgcgcct gcacgttctc cgtcgccgac ggcatcgtcc cgggcaacga aggccgcgcc
    14761 tacgtgctgc gccgcatctg ccgccgcgcc atccgccacg gctacaagct cggcgcccgc
    14821 aagcccttct tctacaagct cgtcgacgcc gttcgcgccg aaatggccga agcctatccg
    14881 gaaatcaaca atccgcgcat cgagaaggtc atcgaagaag aggaacgccg cttcttcctc
    14941 acgctctcca agggcatgga aatcctcgac gccgccattg ccggctccaa ggacggcgtg
    15001 cttgacggcg aagtcgtctt caagctgcac gacacttacg gcttccccgc cgacctgacg
    15061 ggcgacgtct gccgcgaacg cgacgtcaag atcgacttcg acggctttga cgccgccatg
    15121 gcccgccagc gcgaggccgc ccgcgccagc gccaagttca agatggccgc cggcctcgaa
    15181 tacacgggcg ccgacacggt cttcaccggc tacgaaacgc tcgaagagaa cgaagccacg
    15241 gttctcgccg tctacaagga cgggcaggcc gtcgaactcg ccgaagcggg cgacgactgc
    15301 atcgtcgtct tcgaccgcac cccgttctat gccgaacagg gcggccaggt cggcgaccgc
    15361 ggccactgtc gcaacgacgc ctccctcgcc ggcgtgctcg acacgatccg cgtcaagggc
    15421 aaggtcacgg gccactcctg ccacgtcgag gaaggccgca tcgccgtggg cgacaagttc
    15481 cgcctgacgg tcgacgccga ccgccgccac gccacgcagc gcaaccactc agcgacccac
    15541 cttctccagc aggccctgcg taaggtcctc ggcgagcacg tcgaacagaa gggctccctc
    15601 gtctccccga ccgagctgcg cttcgacttc tcccacagcc aggccatgac gaccgagcag
    15661 atcgcccgcg tcgaagacct cgtcaacgag cagatcctcg ccaacacggc cacgaaggcc
    15721 gaagtcctgc cgcttgaaga agcccgcaag accggcgcca cgatgctctt cggcgaaaag
    15781 tacggcgaca tcgtccgcgt tgagacgatc ggcaactccg tcgagctctg cggcggcacg
    15841 cacgtcgtgc gcacgggcga catcggctcc ttcaagatcc tcggcgaagc gggcgttgcc
    15901 gccggcatcc gccgcatcga ggccgtcacg ggcatgaacg cccttgccga agcccagaag
    15961 aacgccgccc tcctcggcca ggccgccttc cgcttcaacg tcaaggcctg cgacctgccg
    16021 tccaagatcg acgacatgat ggccaacgtc aaggccctcg aaaagcagct cgaacagctc
    16081 aagagccaga tggccagccg catgggcgac acgctccttg aaaaggccat cgaggtgaag
    16141 ggcatcaagg tcctcgccgc ccgcatggac ggcgccgacg ccaaggccct gcgcgaaacg
    16201 atggacaagg tcaaggacaa gctcggcacc gccgtggccg tcctcgccgc cgtcagcgcc
    16261 gaaggccgcg tccagctcgc cgcgggcgtc agccgcgacc tcaccgaccg cgtcaaggcc
    16321 ggcgaactcg tcaactccgt cgcgcagaag ctcggcggca agggcggcgg caagcccgac
    16381 atggccatgg ccggcgccca gagcgccgaa ggcctagacg ccgcgctcgc cgacgttcag
    16441 gcctgggtcg aagccaagct ctgaggacgc acgatgaccg aaacgctcga agtcgtcgac
    16501 ctctgcctgc ccggcctcaa ctgcccgatg ccggtgctgc gcgccaagaa ggccctcgcc
    16561 aagatggcgg gcggcaccct cctgcgcgtc gtcagcaccg acaggcacag cctggccgat
    16621 ctggcggaat tctgccgcca gacgggccac gagctcgtga gccagaacac ggacgaggcg
    16681 gcgggcgaat acgtcaccgt catcaggcgc cgcgaggact gacctccggc cggtccgaac
    16741 cctgatcaag gtccctgaag catccgcttc gggggccttt tttcatgcca gcccgaaaag
    16801 actgtcccgc aagcaaatcg actgaacagg aaggaaacag ataccacatt tgcggtattt
    16861 tcacaaaaag ccttgccttt tttcttcggg ttcgctaata ttctgctcct acagttttga
    16921 cgcggtagta gctcagttgg atagagtatc tggctacgaa ccagagggtc gtgggttcga
    16981 atcctgccta ccgcaccatc aactctgtag aacacaagtg tgcggtagta gctcagttgg
    17041 atagagtatc tggctacgaa ccagagggtc gtgggttcga atcctgccta ccgcaccagc
    17101 cattctggag ccgctgtttt cgtctgaaaa cagcggcttt ttcgttacac tttttcaggc
    17161 gatttcaggc cttctaattt ttcagcacga aactcctgtg gacaacccac gtttgcacaa
    17221 aatttatgca aactgcatca atcatatttt gcagggttat tcccgtagaa gaccccgctt
    17281 tttcaacctt tatgcacatt ttctgtggat aacttaccca caaccccgtg catgaggtct
    17341 tcattcgcat gaaaaggccc gcgtggaagc tgcgttccat gcgggccctg tcttttcctg
    17401 cgtccgtcct caggaggtcg tcaggcgcgg cgttcgcagc ggcagaaggt cacctcgaag
    17461 gagcgggact cggtcggctc gagcatttcg agcacctcgg tggtccattc ggactcgggg
    17521 agctcgggga aggtcgtgtc gccctcgaag tcggcgtcga tcttcgtcag ccacacgacg
    17581 tcggccgacg gaagagcctg acggtaaatc tcgccgccgc cgagcacgaa gacggtgtcc
    17641 gtgtcagcga gcgtcctgaa ggcctcctcg agggaaggaa ccacttcggc gccttcggcg
    17701 cggtagtccg cattgcgcgt gacgaccacg ttgcggcggc cgggaagcgg acggccgatg
    17761 gactcccagg tcttgcggcc catgacgatg gggtggccca tcgtggtgcg cttgaagtgg
    17821 gcgaagtcct cgggcacgcg ccagagcatc ttgttgccgc cgccgatgac gccgttgcgt
    17881 gcggctgcga cgatgagtgc gatcttcatg agtctctcct cagacggcga cgggcgcctt
    17941 gatgtggggc cacggatcgt agccggtgat ttcgaagtcc tcgaacctgt agtcgaagat
    18001 ggagtcgggc ttgcgcctga tcacgagctg cggataggga cgcggctcgc gggagagctg
    18061 aagctcggtc tgctccatgt gattgtcata gaggtggcag tcgccgcccg tccagacgaa
    18121 gtctccgacc tcgaggcccg cctgctgcgc caccatgtgc gtgagcagcg cgtagctcgc
    18181 gatgttgaag ggcacgccga ggaagatgtc gcaggagcgc tggtagagct ggcaggagag
    18241 cctgccgtcg gccacgtaga actggaaaag gcagtggcag ggcggaagcg ccatctggtc
    18301 cacgaggccc ggattccagg cgcacacgat catgcggcgg ctgtcgggat tggtcctgag
    18361 ctgctgcatc aggttctcga tctggtcgac gtggctgccg tcgggtgcgg gccagctgcg
    18421 ccactggacg ccgtagacgg ggccgaggtc gccgttctcg tcggcccact cgtcccagat
    18481 cgtcacgccg ttgtcgtgca agtacttgat gttggagctg ccctgaagga accagagcag
    18541 ttcgtgaatg atcgagcgaa ggtgaagctt cttcgttgtg acaagcggaa agccttccgc
    18601 cagattgaag cgcatctggt agccgaagac ggatcgcgtg ccggtgcctg tgcggtcgga
    18661 cttgtgcgtg ccgtgttcgt atacgtggcg catcagggtc tcgtactgat tgctcggcac
    18721 ggtgctgtct cccgaatgct ggaaggtccc tccggaatgg cgggacccga atgataggtg
    18781 accacagttt accgaaaatc ggactctcca aaagcaggcc cgccagtcct tcgaccggcg
    18841 ggcctgcatg cttcacgaag cggaagtctg gaggattatt cctgaaccac gaccttcacg
    18901 tcttcgccgt actcgtcctt cgtcgtcgtg tggatcgcaa ggagcgcgaa ccagcggcgc
    18961 agcgtgccgt agatcacgaa gagcatgagg agcatgacga tcacggagag cgtggcaagc
    19021 aggatcttgc cgcctggcag gtactggttg acgatgagcc agatgcccgc ccaaaacgtg
    19081 atgacggtca tgaagaggcc gggcacgccc gtgatccagg cgtacttcgc cttgttcatg
    19141 cgcaggatca cggtggtgcc gatcagaagc gtcacgctgg cgagcagctg gttgcagatg
    19201 ccgaagagcg gccagatgga gccgatgtcg cccgagtaaa cgagatatcc ccaggagccc
    19261 gtgaagagca ccgaggtgat gatcacaccc ggaacccatt ccttgtccgc aaacttcggg
    19321 atgaccttgc cgatcatttc ctgaaggaag aagcggccga cgcgggtgcc ggcgtccacg
    19381 gccgtcagaa tgaagacggc ttcgaacatg atggcgaagt ggtaccagta ggccatcagg
    19441 tgttccatga acgggatctt ggagaagatg tgggccatgc cgacggcgag cgagacgctg
    19501 ccgcccggac gggccatcag actttcctga acctcggctt caaggcgcgg aagctcctgc
    19561 acggccatgc cgagcgcctg gaacttctcg gcgggcgcat tgatggcgaa gtagtcggcg
    19621 ggcaccagca cgcaggcggc gacaagggcc atgatggcca cgaagccttc catcagcatg
    19681 gcgccgtagc ccacgaagag cacgtcgcgt tcgttgccga ccatcttcgg ggtcgtaccc
    19741 gtgccgatcg tggcgtggaa gcccgagagc gcgccgcagg cgatcgtgat gaagatgaac
    19801 ggaatgacgg gaccgcccac gatcgggccg ccgccgtgga tgaagtccgt gaaggccggc
    19861 atctggaagt tcggcatcac gaaggcgatg ccgatggcga gggcgccgat cgtgccgatc
    19921 ttgaggaagg tggagaggta gtcgcgcggc agcagcagga gccacacggg aagaacggag
    19981 gcgaagaagc cgtagatcgg gatgaggatg ctcatcgcct tgcggtcgat gtcgagccag
    20041 ccgaagtatt cgggatgggc ggcgacccac ggaccggaaa ggatgcagag gaagaggagc
    20101 acgatgccga tgaccgtcgc gccgaccacg tcgcccttgc gccagatctg catgtagagg
    20161 cccatcagaa tggcgatggg aatcgtcgcg gcgaccgtgt aggtcgacca gagcgagttg
    20221 tgcatggcgt tgaccacggc gatcgagagg cccgcgagcg tcaggatcag aatcgcgagg
    20281 acggcccacg aggcgacgaa gcccgtcgtc ctgtcgatct cctgcgtcgc gatgtaggcg
    20341 agcgagcggc cgcggtggcg cacggagcag aagagcacga ccatgtcgtg cacgccgcct
    20401 gcgagcacgc agccgatcag aatccagagc aggcccggca tgtagccgaa ctgcgctgcg
    20461 agaactgggc cgagaagcgg gccggctgcc gcaatggctg cgaaatggtg gccaaagagc
    20521 acgtacttgt tggtcgggac atagtcctgc ccgtcgctgt acttgacggc aggcgtcttg
    20581 cgggcttcat tgaggctgag gaccttggtc gcgatgaaga gaccgtagaa gcggtaagca
    20641 atggcaaaga tacagagcgc ggcaaagacg attgtcagtg cgttgactcc cgttcctgtt
    20701 tccatgtttt tctccgggtt ggctttgggg ggatttcgtc ggcttcaagc gtctttccgg
    20761 aacacgaaaa aatggcctga aggatccggt aggtgcggat cccaggccat attgacgggc
    20821 ccggcgctct caaactcgcc gatctgcctg tcagtctatc gttttcaagg ttttgcgcgc
    20881 aaatggtgcg cgtgattttt tcctcagtgt ttttgcgcac ccttgaagat catgaatgcc
    20941 atcgggagct caagagaggg ctttgcacca ctgaaaacac cgctccaacc gatcatgaag
    21001 tcgatgtact ccttgggctc gatcttgcgg ccgagcatct tctcatgcgc atcgcgcgcc
    21061 gtcagaagct cgaggtcctt gagatagcag gagagcgcaa gacggatcac gacctcatcg
    21121 gacatggcgt ccgtgccgcc ctcaagaccc tgagcggccg tctcggcggc aagttcctga
    21181 cgaagcgagg cgcgcaggtg cttcgcaagc tcggcgcccg cccagccgtt ctgcacgatg
    21241 cgggactcgt caccgcccgt cgaaagaagc atcaggatgt cggcgaggaa ttcctcggcc
    21301 tgctcgtgga cggcggccgc atccttgtgg ccggggtcgt tgctgcggat ccaggcgtcc
    21361 tgcatgcagg cgattccgag ctcctcgagc gcaaggaaca tgctgttctg gtcgcgaagg
    21421 tcgatggcgg cgtgaggcgc gaattcagcg tcacgggctt gctggggcat gtctgtctcc
    21481 gaagtcaaag agtgttttga aagggtatcg gcattgtaca acgccgcgct gcgctgcaga
    21541 aaaggagctc agttgagccg cctagagacc gcatcaggga gaggcatcac gggaatgccc
    21601 tcctcatgca acgcctgagc ctcctcgccc gaacaggtgc ccttcaccag gcgcctctcg
    21661 gcgcgcccgt catgaatgtc tctcacagtt tcgggaaagc gctcgccgac gtcctcggcc
    21721 atcgcggcgg cctcacgcag caccttcatg gcgtccgcct gcatttcgcg ggccctctcc
    21781 cagccgcggg cggcgacgtc ggccgcaacg tccgtcctcg tcgtcccctg cactctgaca
    21841 aagttgggag cgctcgggcg cttggcaacg cacggatctc cgcacacggg acactcgatg
    21901 cggccttcct tcaggtccgc ctccagctcc tcatcggagc gaaaccatcc ctcgaacaca
    21961 tgtccgtgac cgcagcacag gttgcagatc ttgattccca tcaccgtcct cccgcacgcc
    22021 cttgaggcag gagccgaaaa aagttcacac gactttaaaa atatactgta caattgcgct
    22081 ctctggcgaa accgtcagct cgctccatct tggaagggtg gcagagtggt tgaatgtacc
    22141 gccctggaaa ggcggcatac ggaaattccg tatcgagggt tcgaatccct c
//
LOCUS       DBMDTI010000008        21884 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_809_length_21884_cov_3.260937, whole genome shotgun sequence.
ACCESSION   DBMDTI010000008 DBMDTI010000000
VERSION     DBMDTI010000008.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 21884)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21884)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21884
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_809_length_21884_cov_3.260937"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="ACZPB6_01205"
     CDS             complement(<1..642)
                     /locus_tag="ACZPB6_01205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1183127.1"
                     /translation="MNSVEQKTVPVSLVVIVKNSASSLRRCLESAPFVDEILVVDSGS
                     TDDTVEVARECGARVLFQDWLGFGPQKKFAVNQAKNDWVLCLDADEYLSDGLRESMFR
                     LFQSEPSHKAYRFARCNLFLGRFLRHGEGYPDWSLRLFNKRAANWTEDLVHEKVEGQG
                     GRLKVGTLSGDLMHESCESVARYIEKQRAKYQLPGAVFAAAAWMIFSGIFSVYV"
     gene            complement(629..2317)
                     /gene="recN"
                     /locus_tag="ACZPB6_01210"
     CDS             complement(629..2317)
                     /gene="recN"
                     /locus_tag="ACZPB6_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR1183128.1"
                     /translation="MLTALSLRDFVIVESLSLDVRRGFTVLTGETGAGKSILIDALQL
                     ILGGRADAGVVREGAERTNIVAEFQPPESARKWLAANELETGDDMLMIRRTVDVKGRS
                     RAWVNGVSVAASQLRELGETLIDVHGQHAHQSLLKPAYQLKLLDDHAGTFMELSAVRE
                     AFTVWQKARRALDDATANADAIAEKAERLAWMIEDLEALSPRGGEWESLNEDHRRLSH
                     GVAIAEGLNEVLGTLTEDGESASAMLSSAHAKLSSLSRYDAKLASVAETLSTGIDLIE
                     EAARDAARYLDRADLDGERFAEVDRRVSHYYELARKFRTEPESLHELLEKSRSELKVL
                     TGAKDVEALRKAEASARGSYDVAAASLSEARRHGALALGEAVTAQMQRLAMKGARLEI
                     ALVNQPPSASGSEHCEFLIAGHAGVQTRPLIKVASGGELARISLAIAVITAAAMPVPT
                     LIFDEVDSGIGGATAEVVGQLLHRLGESRQVLCVTHLPQVAACGDNHWRVEKQFTGET
                     TRSSLRVLTARERVEEVARMLAGISISDNTRAVAQEMLLAGTTPNSKEVCDEQC"
     gene            complement(2359..3267)
                     /locus_tag="ACZPB6_01215"
     CDS             complement(2359..3267)
                     /locus_tag="ACZPB6_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR1183129.1"
                     /translation="MTSAFKKVAVFVKPTEIMVVPLQALIKVIEACGAEVFLCERSAA
                     SLKGRSNRPGHPRAVLGAMCDLAIVLGGDGTMLGVARDLASFDIPIIGVNAGRLGFIT
                     DIVLEDMETILPQVLAGRYTRDVRRMLACEVVRKGKSIFSGIAVNDVGISHGRAGGMV
                     EFVIYVNGQQMSSQSADGIICSTSTGSTAYSLAAGGPILHPSLQGLCLVPVAPHTLSN
                     RPIVLPANVAIDIELTNARDAVAYCDMQEFFDVQPGDVLKIRPTEMTMTMLHPAGYDY
                     FDLLRRKLKWNFMPTSVKRRTNSSGS"
     gene            complement(3401..4195)
                     /gene="fabI"
                     /locus_tag="ACZPB6_01220"
     CDS             complement(3401..4195)
                     /gene="fabI"
                     /locus_tag="ACZPB6_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR1183130.1"
                     /translation="MGFLNGKKLLITGVISNRSIAYGIAKACRREGAELAFTYANERY
                     RERIEGLAADFDSKLVYRMDVGSDEDIAAVVEELGKAWGGLDGFVHSIGFAPREAIAG
                     DFLEGLSRESFKIAMDISAYSYPAMAKAFLPLMKGRQASVLTLTYLGSERVVPNYNTM
                     GLAKAALESSTRYLAASLGREGIRANAISAGPIKTLAASGIQDFGKLLHVMEQAAPLG
                     RTVTTEEVGNTAAFLLSDLASGLTGDVVYVDAGFHAMGAAMIEVEK"
     gene            complement(4374..6524)
                     /locus_tag="ACZPB6_01225"
     CDS             complement(4374..6524)
                     /locus_tag="ACZPB6_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR1183131.1"
                     /translation="MIRPLQIRPFLLKTLPLLSLGFTFLLFEPAAGIPKAAAAAGVPA
                     ADEAATVAQAASDEPVGTVEALGPPVDVWDRIRRGYAMPALENDKVDRWLEYYRRKPD
                     YLNRMFERSGRYLYHILEEVENRGMPTELALLPFVESAFQPEALSRAKAAGLWQFMPA
                     TGTHYSLEQNLWRDDRRDVLESTRAALDYFEYLYGMFSDWHLALAAYNWGEGSVQRAI
                     RRQQARKRPADYQHLRMPNETANYVPKLEAIKRIVTDPSKYGVKLPDVGNEPFFVTVT
                     KPRDIDTETAAELAGMPLKEFRQLNPSFKLPVIVASHNNVMLLPADKVDEFIDNLASW
                     MDGGQPLSRWTTYKLQEGETLASVAEAAGMTEDELRDVNGIPKGRRVLANSTLLVRAN
                     ADDQTDIAAETADAKLRLSPLTTWRRVTYRVRKGDTLSGIARRWHITKKSIVQANRLR
                     SQNLRVGQRLILTVPNVERAPIRTVSTDSGAKHVIHAVKAGETLGSISRRYGVSTAIL
                     RATNRISGNTIRAGQRLRILTVASVEESDVRVHTVRKGDTLSEIADHYDVSVVRLKRA
                     NRLTGNALRVGQRLEIPTGSEPKAPAVQPSRADESPEAGLYRVKSGDTLYEIAGRFGV
                     SVNALKAVNGLSGSSLRAGQKLVIPATAKTQPSRADSQPAGSAQAQDTVYRVKSGDTL
                     SSIASRFGTSVRILKDLNGLRSNRLSVGQRIRLP"
     gene            6670..7449
                     /locus_tag="ACZPB6_01230"
     CDS             6670..7449
                     /locus_tag="ACZPB6_01230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1183132.1"
                     /translation="MNSFSFADFLKTPQGETLHAWESSQYDEFSADAFGREALQVGAP
                     QLDTLRANGITSQWLTSESYDELDNRLLRPTLQTVQAESGWLPFPAESLDLVTLPHAL
                     DFAPSAHQTLREAARVLVPEGRLILTVFNPLSLWWMRQKAVGAGLRPYLPTHTSPVPL
                     YRLKDWLTLLGFEIDRGRFGVYSPSCHSLRNFERWKWLDKAGDRWAPHCANLIVLCAV
                     KRLPGMKLVGRLPMKSPLDILTAGSPLPVPKEARRQAGHGN"
     gene            7493..7966
                     /gene="rnhA"
                     /locus_tag="ACZPB6_01235"
     CDS             7493..7966
                     /gene="rnhA"
                     /locus_tag="ACZPB6_01235"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIR1183133.1"
                     /translation="MAQQTEKTLEIWTDGACKCNPGIGGWGAYMVWGAHTREMYGGSK
                     LTTNNQMELTAVISALSALRRKCPIIIHTDSSYVKDGITKWIHGWKRNGWRTADKKPV
                     KNAELWQQLDELTSQFDIEWRWVKGHAGDPGNEKADELADMGCEVAAGTRPAPSY"
     gene            8051..8125
                     /locus_tag="ACZPB6_01240"
     tRNA            8051..8125
                     /locus_tag="ACZPB6_01240"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8083..8085,aa:Val,seq:cac)
     gene            complement(8248..8706)
                     /gene="fur"
                     /locus_tag="ACZPB6_01245"
     CDS             complement(8248..8706)
                     /gene="fur"
                     /locus_tag="ACZPB6_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HIR1183134.1"
                     /translation="MNDTSGNQTSELKNAGLKATAARLRILELFQKCAEEKNGERRHI
                     SAEDLYKELVSTGVDIGLATVYRVLAQFEQAGLIVRHHFDTDRATYELEDGGHHDHLV
                     CLQCGKVVEFVAPSIEKEQAAVAKRYGYELSDHTMVLYGLCADCRKKSAG"
     gene            8798..9268
                     /locus_tag="ACZPB6_01250"
     CDS             8798..9268
                     /locus_tag="ACZPB6_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIR1183135.1"
                     /translation="MLANRFSLAALGMTAALVLTGCNTVSEAWDSPRSVVPEFLQPYR
                     ADVHQGNLITSEMVLQLEKGMTQPQVQFLLGIPLLRDQFHPYRWDYIYYLLRGNDDRQ
                     LRKLTVWFNEDGRVDHWTSDPMPDEEQADQMILGTTDTFEPRAPQVDPGVAAQH"
     gene            9293..9499
                     /locus_tag="ACZPB6_01255"
     CDS             9293..9499
                     /locus_tag="ACZPB6_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183136.1"
                     /translation="MNPQIERLQALVAQLIARLEHERSESRRLASQLASREAELAQTK
                     HRLEDMRRKVEALFSASRSGDPRL"
     gene            9511..9933
                     /locus_tag="ACZPB6_01260"
     CDS             9511..9933
                     /locus_tag="ACZPB6_01260"
                     /inference="COORDINATES: protein motif:HMM:NF017016.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR1183137.1"
                     /translation="MPEVTVNIYSRSYRLAVSTGEEALIQRCADIVDKQMKAIREGGK
                     VINQDQIAVLAALEVAYEANKNVQKNSTDAQRSAAEKDAEIARLKARIAELESRPAPV
                     AAPLQASGPDAETTEAIERLCRQCEQAIYANMSRGAVL"
     gene            10233..10733
                     /locus_tag="ACZPB6_01265"
     CDS             10233..10733
                     /locus_tag="ACZPB6_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HIR1183138.1"
                     /translation="MNNTTVTEDLLRTASRFWLMKSEPGECSIDDALAAPGRRVSWFG
                     IRNYQARNFMRDDMTIGDAVLFYHSSCAKPGIAGIARIASDVYPDELQFDPESEYYDP
                     KSTKENPRWLTLDVEALFKCPVVEIGRLREEPELAGMRVLQKGNRLSITPVEKEEFAF
                     IVRNLL"
     gene            complement(10964..11755)
                     /gene="lgt"
                     /locus_tag="ACZPB6_01270"
     CDS             complement(10964..11755)
                     /gene="lgt"
                     /locus_tag="ACZPB6_01270"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR1183139.1"
                     /translation="MLIHPQFDPVAFSLGPLAVRWYGLMYLAGFFCFWLLGRRRSSEP
                     WRGISGEDLENLLFYGIFGVILGGRLGYCLFYQPEFYLSHSMQILHVWEGGMSAHGGL
                     LGVIAVMILYGRLHGISFWTISDFVAPLAPLGLMLGRIGNFINGELWGRPADASLPWA
                     MIFPQAGDGVPRHPSQLYEALGEGLLLFILLWWASSRPRAAGFVSGLFCAGYGVARFV
                     VEWFREPDAFLGLQALDLSRGQWLTLPLIVLGIAVMVWAGRRNRA"
     gene            11855..12652
                     /gene="thiM"
                     /locus_tag="ACZPB6_01275"
     CDS             11855..12652
                     /gene="thiM"
                     /locus_tag="ACZPB6_01275"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HIR1183140.1"
                     /translation="MTAPLLSGVDAARHLELLRTARPLVHCLTNEVVQEITANVLLAA
                     GASPAMVVAEEEAGFFAGIAGGVLINIGTPYPSRLRAMHASADAARAAGRPWVLDPVA
                     AGGIPWRDGIIREFVEKQPTVIRGNASEILALAGEKSGGKGVDSTDSSDAAVEGAMRL
                     AGSSGSIVVVTGETDYVTDGVRTLRVTGGDPMATLVVGTGCSLSALVAAFCAVSDDRL
                     TASATACALAKKAAEAARRRSEGPGSFHNAYLDGLHLIKPADFTSAE"
     gene            12661..13305
                     /gene="thiE"
                     /locus_tag="ACZPB6_01280"
     CDS             12661..13305
                     /gene="thiE"
                     /locus_tag="ACZPB6_01280"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR1183141.1"
                     /translation="MSDNYRVLRALRLYLVLDPGLCGGSEGLVRTAQAAAAAGVTAVQ
                     LRAPDWKKRLYAECGRELLKALAPYDVPLFINDHADVAVAIGAHGVHVGQSDLSPEDC
                     HRLMTPGMMLGLSVSNVAECLGVDPALVDYIGIGPYRATPTKKDAAAEIGLSGLTDIV
                     SRAPCPSVAIGGIKAADVGSVMQSGVDGVAVVSAICGTPDIPASVKALLDAMPS"
     gene            13420..14883
                     /locus_tag="ACZPB6_01285"
     CDS             13420..14883
                     /locus_tag="ACZPB6_01285"
                     /inference="COORDINATES: protein motif:HMM:NF022518.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HIR1183142.1"
                     /translation="MSDSTPKFVHLSESDARDVLFAEMLEKRAGGDGRWSAGNRDAAT
                     RDAKRLAGEGVGARDFLPLRARLVAARLHEDPANAFGTTSFRRASLALTVILGLAALM
                     SGMLTDHLATEGARINLLSPPLLLLIVWNLAVYLLIILKPLMPSGTSRRPLSVREFLS
                     RSLLKAAGSSAVSVEAGTALFLPQCRWLVARALHIAAILFAAGLLAGMAVRGIGTAYH
                     VGWESTWLADNPEAVHTVLNTIYGHLPFCPPVPDVSAVAALAFDAQTASAVDAAPWLL
                     RLMCLLALAIILPRLLLVLKCTVGLAASRRRVRIDLESPYYRSILSVSDAPAVRTTVI
                     VDSTDSVDGLPAHWHDFKAKLKTAEPGSGDVDLLANSAWDGTTSEVLAAVKPAIIHRL
                     LLAFDPSSTPEVEVHGAMMDAAASWCTSKQSPAPVVVLDLSRINRRFGRENPAAGSRT
                     ALWQTFAAERGLRTLATDMSIPEDVDRICTLLSGQVR"
     gene            14918..16378
                     /locus_tag="ACZPB6_01290"
     CDS             14918..16378
                     /locus_tag="ACZPB6_01290"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HIR1183143.1"
                     /translation="MSDHDQFRIHLSLVSHTNIGKTTLARTLLMRDVGEVADRAHVTE
                     TTDDYVLARDSAGCELVLWDTPGFGNSVALAKRLAGRSNPIGWFLSEIWDRMTNKAFW
                     LNQRAIKHVRDISSVVLYLVNASDLPKTAPYITAEMQILSWIDKPVIVLLNQMGKPRT
                     HAEEQADVAAWREAMAPYPFVKDILPMDAFARCWVQETALFDSIGRALPAQMHSTFDT
                     LRDIWTRSRRALYLSSVDAMARHMWRLLQAHELVPTPTLKDHLRSFGSQIGLIKTRNE
                     ALEDAQTALSAMAADSLCSLTDKLIGINGLTGSGVSREILRRMKTDWQMASHSISPGP
                     AAAVGASVGVAGGAAAGAAADMASAGLSLGLGTLLGGIIGAIGGVGAATIYNSRHRKE
                     GIDISWSERAAQDFMLESLLLYLAVAHFGRGRGDWQEGESPEFWKKAVQDAMTEQKVS
                     FQAIRKLTPEEGVEQLTRMTDSLLRGVFMQLYERSI"
     gene            16384..16755
                     /locus_tag="ACZPB6_01295"
     CDS             16384..16755
                     /locus_tag="ACZPB6_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HIR1183144.1"
                     /translation="MLSKGFATIGLLLVSNVFMTLAWYAHLKLQSAKLISNWPLYTVV
                     LFSWAIALLEYSFMVPANRIGFEGNGGPFSLIQLKIIQEVITLTVFTLFSVIAFQQHL
                     AWNHFAAFACLVAAVYFVFMK"
     gene            complement(16856..17788)
                     /gene="secF"
                     /locus_tag="ACZPB6_01300"
     CDS             complement(16856..17788)
                     /gene="secF"
                     /locus_tag="ACZPB6_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR1183145.1"
                     /translation="MEFFRIHKTIPFMHYRHVLNLISLVSFIVAVFWIFTHGLAFSIE
                     FTGGTQMEVRYPSAANTEQVRQDLKAVSNEVLVQTFGTASDIVIRVPTKQGYTSGQVA
                     EEVMKVIHQKTPEAQLTRTEYVGPQVGEELARDGGTALALVVAGIMIYLAFRFEWKFS
                     VAAIIANLHDIFIVLGIFSVMQWEFSLPVLAAVLAVLGYSVNESVIIFDRVREHFRTM
                     RRADTMEVINSAITATISRTVITHTSTLCMTLSMFFFGGPALHYFSLALTIGILLGVY
                     SSVFVAAAIALYLGVKREDLVRPVKKDEVEEMVP"
     gene            complement(17804..19696)
                     /gene="secD"
                     /locus_tag="ACZPB6_01305"
     CDS             complement(17804..19696)
                     /gene="secD"
                     /locus_tag="ACZPB6_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR1183146.1"
                     /translation="MNRYPLWKYIVIVVALVIGLVYTLPNFYGESPAVQVSSGKATVK
                     VTEQTLKTVEDLLRNADLHPNGIFFEQGAQQNTVRVRFEPTEAEKQLQAREVLEKALN
                     PNREDPAYVVALNLTPNTPQWLLSIRALPMYLGLDLRGGVHFLLQVDMRAAITKRAEA
                     TLADLRTQLRDKRIRHTGMNRVGNVVEVSFASEEERARANDLLRNTQPDLVLSLPEAS
                     AAPYLLRAELSQKAVQNVQSYALKQNISTLHNRINELGVAEPVIAQQGADRIVVQLPG
                     VQDTAKAKDILGRTATLEVRLVDDSPEALAQLAQGNVPFGDEKFMDREGNVLLVKRRV
                     ILTGENLNDAQPGFDSQTQEPTVNLELDSRGARIFQDVTRDNVGKRIAIILFEKGKGE
                     VVTAPVVRQEIGGGRVQISGRMSTVEATDTALLLRAGSLAAPMEIVEERLIGPSLGEA
                     NISAGFKSTLYGFIVIALFMAVYYQVFGLVSAASLVCNVMMLIALLSMLQATLTLPGI
                     AAIALTLGMAVDSNVLINERIREELRLGRLPQTAISEGYDRAFATILDSNITSLIAGL
                     ALLIFGSGPVRGFAVVHCLGIMTSIFTSVVVSRSMINLIYGRQKKLTKVHIGQIWRPD
                     AEKTAR"
     gene            complement(19877..20224)
                     /gene="yajC"
                     /locus_tag="ACZPB6_01310"
     CDS             complement(19877..20224)
                     /gene="yajC"
                     /locus_tag="ACZPB6_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR1183147.1"
                     /translation="MFFVSDAMAAGEAAAQVGGMEGFLMQVVPLVVIFAVFWFFLIRP
                     QQKRQKEHMQMCEGLTRGDEVTTLGGISGRIESVDEQTIGLQVASVDGKPVVIRMQRT
                     SVSVVLPKGTLKI"
     gene            complement(20349..21482)
                     /gene="tgt"
                     /locus_tag="ACZPB6_01315"
     CDS             complement(20349..21482)
                     /gene="tgt"
                     /locus_tag="ACZPB6_01315"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR1183148.1"
                     /translation="MAFDFKVKATCGRARRGELTLNHGVVQTPIFMPVGTYGSVKAMA
                     PYELKEIGSQIILGNTFHLWLRPGLEVIGAHNGLHDFMQWDKPILTDSGGFQVFSLGE
                     LRKITEEGVKFSNPINGDKLFLSPEVSMKIQRVLNSDVVMVLDECTPYKIGDRPATEL
                     EAAKSMRMSLRWAKRSRDEFDRLENPNALFGIVQGGMFEHLREESLEGLKDIGFHGYA
                     VGGLSVGEPKEDMLRIMDHIVHKLPDDRPRYLMGVGTPEDLVAGVSQGIDMFDCVMPT
                     RNARNGWLFTRFGDVKLKNSRYRNDLRPLDPSCNCYTCRNFTRAYLHHLHKVGEILGA
                     RLNTIHNLSFYLQIMQEMRDSLEAGTFEAWKKQFAEDRARGVE"
     gene            complement(21528..>21884)
                     /locus_tag="ACZPB6_01320"
     CDS             complement(21528..>21884)
                     /locus_tag="ACZPB6_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HIR1183149.1"
                     /translation="NMPEDVAARINRAKAEGRRVIAVGTTSLRTIESAADAVGHVAAG
                     ARDTSLFIMPGYRFRIIDALITNFHLPKSTLVMLVSALAGRERILEAYRHAVEEKYRF
                     FSYGDACFIENARLAG"
BASE COUNT         4331 a         6989 c         6508 g         4056 t
ORIGIN      
        1 cacgtacaca gaaaagattc ccgaaaaaat catccatgca gccgccgcaa ataccgctcc
       61 cggcagctgg tactttgccc tctgcttctc aatatatcgg gcaacgctct cgcaggactc
      121 gtgcatcaag tccccagaca aagtgccgac tttcagcctt ccgccctgcc cctcgacctt
      181 ttcatggaca agatcctcag tccagttggc cgctcttttg ttgaaaagac gcaacgacca
      241 gtctggatac ccttcaccat gcctgaggaa acggcctaaa aacagattgc atcgggcaaa
      301 gcgatatgct ttgtgagaag gctcgctctg aaagagcctg aacattgact cgcggagccc
      361 gtcggacagg tattcgtccg catcaagaca caacacccag tcatttttag cctgattgac
      421 tgcaaacttc ttttgcggcc caaatccaag ccagtcttga aaaagaacgc gagcgccgca
      481 ttctcgggcc acctcaaccg tgtcatcagt gcttcccgag tcgacgacca gaatctcgtc
      541 cacaaacggc gcactttcga ggcaacgcct gaggcttgaa gccgagttct tgacaatgac
      601 gacaagagaa acgggcacgg tcttttgttc aacactgttc atcacacact tccttagaat
      661 tcggcgtcgt cccggccaga agcatttcct gtgccacggc acgcgtattg tcggaaatcg
      721 agatgcctgc cagcatgcgg gccacctcct ccacgcgctc ccgtgccgtc agaacgcgca
      781 gactgcttcg agtggtctcg cccgtaaact gtttctccac gcgccagtga ttgtcaccgc
      841 acgcagccac ctgaggcaga tgcgtcacac agaggacctg acggctttcg ccgagtctgt
      901 gcagaagctg tcccaccacc tcggcggttg cgccgccaat gccgctgtcg acctcgtcga
      961 aaatgagcgt ggggacgggc atggcggccg ccgtgatcac ggcaatggca aggctgatac
     1021 gagcaagctc gccgccggaa gcgaccttga tcaggggccg agtctgaacg cctgcgtggc
     1081 cggcaatgag aaactcgcaa tgctccgacc ccgatgcact aggtggctga ttcacaagcg
     1141 cgatctccag acgggccccc ttcatcgcca gacgctgcat ctgagccgtc acggcctcgc
     1201 ccagcgccag cgcgccgtgc ctgcgcgctt cagacagact tgccgccgca acgtcataag
     1261 aaccgcgcgc cgatgcctcc gcctttcgca gagcctcgac gtccttggcg cccgtcagca
     1321 ccttgagttc cgaccggctt ttttcgagca gctcgtgcag gctttctggc tccgtcctga
     1381 acttacgggc aagctcgtag tagtgggaga cgcggcgatc aacctcggca aagcgttcgc
     1441 catccagatc ggcacggtcg agataacggg cggcgtcacg tgccgcctcc tcaatgaggt
     1501 ctatgcccgt cgagagcgtt tcagcaactg aagcaagctt ggcatcgtag cggctcagcg
     1561 aagagagctt tgcgtgtgcc gaggaaagca tggcggaagc ggactctcca tcctccgtca
     1621 gggttccgag cacctcgttc aaaccctcgg caatcgcaac gccgtgcgaa aggcgcctgt
     1681 ggtcctcatt gagggactcc cattcgccgc ctcttggcga cagcgcctcc agatcctcga
     1741 tcatccaggc gaggcgctcg gccttttccg caatcgcatc ggcgttggca gtcgcgtcat
     1801 caagagcgcg ccgggccttc tgccagacgg tgaaggcctc tctgacggca gagagttcca
     1861 tgaacgtacc tgcatgatcg tccagaagct tgagctgata ggcgggcttc agaagcgact
     1921 gatgcgcatg ctgcccatgt acgtcaatga gcgtttcgcc gagttccctc agctgggaag
     1981 ccgccaccga gacgccgttc acccaggcgc gggagcgtcc cttgacgtcg accgtcctgc
     2041 ggatcatcag catgtcgtcg cccgtttcaa gctcgttggc cgcaagccat tttcgggcgc
     2101 tctcaggcgg ctgaaactcc gccacgatgt tcgtgcgctc agcaccctca cggacgacgc
     2161 cggcatccgc ccgaccacca agaatcagct gcagggcgtc gatgagaatg gacttgccgg
     2221 cgccggtttc acccgtcagg actgtaaagc cccgccggac atccagactc agggactcga
     2281 cgatcacaaa gtctctaaga ctcaaggcag tcagcatgaa ggcgtatctc cggaagcagt
     2341 attgaaggag ctactctatc agcttcccga ggaattcgtg cgcctcttca cgctcgtggg
     2401 catgaagttc cacttgagct tgcggcgcag cagatcgaag tagtcgtatc ccgcaggatg
     2461 aagcatcgtc atggtcatct cggtcggacg gatcttgagt acgtcgccgg gctgcacgtc
     2521 gaagaactcc tgcatgtcgc agtaggcgac tgcatctcgc gcgttcgtga gctcgatgtc
     2581 gatcgccacg tttgccggga gcacgatggg gcggttcgag agcgtgtgcg gcgcgacggg
     2641 cacgaggcac agcccctgaa gagacggatg caggatcgga ccgcctgcgg cgagcgaata
     2701 ggccgtggac cccgtcgaag tcgagcagat gatgccgtcg gccgactggc tcgacatctg
     2761 ctgcccgttc acgtagatca cgaactcgac catgccgcct gcacggccgt ggctgatgcc
     2821 gacgtcgttg acggcaatgc ccgagaagat cgacttgccc ttgcgcacga cctcgcacgc
     2881 gagcatgcgg cgcacgtcgc gcgtgtagcg tcccgctagc acctgtggca gaatcgtctc
     2941 catgtcctca agcacgatgt ccgtgatgaa gccgagacgc cccgcgttga caccgatgat
     3001 cggaatgtcg aagctcgcca gatcacgagc aacgcccaac atcgtaccgt cgccgccgag
     3061 cacgatcgca aggtcgcaca tggcgccgag tacggccctc ggatgaccgg gacggttgct
     3121 ccgtcccttc agcgatgcgg cgctgcgctc gcagaggaag acctccgcgc cgcaggcctc
     3181 gatcaccttg atcagcgcct gaagcggcac gaccatgatc tcggtcggct tgacgaagac
     3241 cgcgactttc ttgaatgcag atgtcatggg catgggaatg cgtaaattgg gaccacaatc
     3301 agaagcacta taaccctgca tgtgatcaaa aataaagcac aaaaagcggg aaccgactct
     3361 ggatcgatcc ccgctcaggt gcactgtcaa taaactgcgc ttacttttcc acttcgatca
     3421 tcgcggcgcc catggcatgg aagcccgcat cgacgtagac gacgtcgccc gtcagaccgc
     3481 ttgcgaggtc ggacagcagg aaggcggccg tgttgccgac ttcctccgtc gtcacagtgc
     3541 ggccgagcgg cgctgcctgc tccatcacgt gcagaagctt gccgaagtcc tgaatgccgg
     3601 aagcggcaag cgtcttgatg ggacctgcgg agatggcgtt cgcgcgaatg ccctccctgc
     3661 cgaggctggc ggccagatag cgggtgctcg actcgagtgc cgccttggca aggcccatcg
     3721 tgttgtagtt gggaacgacg cgctcgctgc cgaggtaggt cagcgtgagg acggatgcct
     3781 gacggccctt catgagcggc aggaaggcct tggccatcgc aggataggag taggccgaga
     3841 tgtccatggc gatcttgaag gactcgcggg agaggccctc gaggaagtcg cccgcgatgg
     3901 cctcgcgcgg cgcaaagccg atcgagtgca cgaagccgtc aaggccgccc caggccttgc
     3961 cgagttcttc aacgaccgca gcgatgtcct cgtcgctgcc gacatccatg cgatagacga
     4021 gcttactgtc aaagtcagcg gccagcccct cgatgcgctc gcgatagcgt tcgttggcat
     4081 aggtgaaggc gagctcggcg ccctcgcggc ggcaggcctt ggcaatgccg tacgcaatcg
     4141 agcggttgga gatcacgcca gtgatcagaa gcttcttgcc gttcagaaat cccataatca
     4201 gtctcacttc ttcagggccg tccgctcctc aagagggacg gacgacgggg ttcaagcatc
     4261 gtttccggca cgtttgccta gccggaacgg gagtgcggtt attgtaggga ctttcgggcg
     4321 gctcgaaaag aaccgcgccc tcccctgcgt tgggtaggcc gttcggtcct cgcctacggc
     4381 agtctgatgc gctgtccgac gctcaacctg ttggaacgaa ggccgttgag atccttcagg
     4441 atccgcaccg aagttccgaa gcggctcgct atgctcgaaa gcgtgtcgcc cgacttcacg
     4501 cgatagaccg tgtcctgagc ctgagcagaa cctgcgggct gagaatcagc tctggagggc
     4561 tgggttttcg cagttgcggg aatgacgagc ttctgccccg cgcgaagcga ggagcccgaa
     4621 agcccgttca cggccttgag ggcattgacg gagacgccga agcggcccgc gatttcatag
     4681 agcgtgtcgc cggacttcac ccgataaagc ccggcctcgg gcgactcgtc ggcgcggctc
     4741 ggctgcactg caggcgcctt gggctcggaa cccgttggaa tctcaagcct ctgccccacc
     4801 cggagtgcat tgcccgtcag gcggttggca cgcttgaggc gaaccacgga cacgtcgtag
     4861 tgatcggcga tttcggagag agtgtcaccc tttcgcacgg tgtgcacgcg gacatcggac
     4921 tcctcaaccg atgccacggt caggatgcgc aggcgctggc ccgccctgat ggtgttgccg
     4981 ctgatgcggt ttgtggctct aaggatggcc gtcgacaccc cgtaccggcg cgagatggat
     5041 ccgagcgtct cgcctgcctt cacggcatga atgacatgct tggcaccact gtccgtgctc
     5101 acggtgcgga tcggcgcacg ctcgacgttg ggcaccgtca ggatcaggcg ctggccaacg
     5161 cgcaggttct gcgaacgcag gcggttggcc tgaacgatgg acttcttcgt gatgtgccag
     5221 cgacgagcga tgcccgagag cgtgtctccc tttcgcacgc ggtaggtcac gcgacgccag
     5281 gtggtgagag gcgacaggcg gagctttgca tcggctgtct cggccgcaat gtccgtctga
     5341 tcatctgcgt tcgccctcac aagaagcgtg gagttggcga gcacgcgacg gcccttcgga
     5401 atgccgttca catcccgaag ctcgtcctcg gtcatccctg cagcttcggc gactgaggca
     5461 agcgtctcgc cctcctgcaa cttgtaggtc gtccaacgcg agagcggctg gccaccgtcc
     5521 atccagcttg caagattgtc gatgaactcg tcgaccttgt cggcaggaag cagcatcacg
     5581 ttgttgtgcg aggcaacgat cacgggcagc ttgaaactgg gattgagctg gcggaactcc
     5641 ttgagcggca ttcccgcgag ctcggcggcc gtttcggtgt caatgtcgcg cggcttcgtc
     5701 accgtgacga agaagggctc gttgcccacg tcgggcaact tcacgccgta cttcgaggga
     5761 tcggtcacaa tgcgcttgat ggcctcgagc ttgggtacat agttggccgt ttcattgggc
     5821 atgcggagat gctggtagtc ggcagggcgc ttcctcgcct gctggcggcg aatggcacgc
     5881 tgcacgcttc cctcgcccca attgtaggcg gcgagcgcca gatgccagtc actgaacata
     5941 ccgtaaagat attcgaagta gtcgagcgct gcgcgcgtgc tctcaagcac gtcgcgcctg
     6001 tcgtcacgcc agagattctg ctccagggag tagtgcgtgc ccgtcgcagg catgaactgc
     6061 cagaggcctg cagccttggc gcgcgaaagc gcctcgggct ggaatgcgct ttccacgaac
     6121 ggcaggagcg caagctccgt gggcatgcca cgattctcga cctcctcaag aatgtgatag
     6181 aggtagcgcc ccgagcgctc gaacatgcgg ttcaaatagt caggcttgcg gcggtagtac
     6241 tcgagccagc gatcgacctt gtcgttctca agggcaggca tggcatagcc tcggcggatg
     6301 cggtcccaga cgtcaacagg aggaccgagt gcctcaaccg tgccgacagg ctcgtccgaa
     6361 gccgcctgcg caacggtcgc agcctcgtcg gccgcaggca cgcctgcagc cgcagccgcc
     6421 ttcggaatgc ccgctgcggg ctcgaaaagc agaaaggtga atccgagact aagaaggggc
     6481 agcgttttca gcagaaacgg acgaatctga agcggtctga tcatggcgca aaaaaatggg
     6541 caggagcgtg tcttgcatcg acaattagtc gattttatca atcgtattga atttacaatg
     6601 ccggagcgcc tcttcaggca caatgagata cacattcacg cagagatgcg cccgatccgc
     6661 acagcagcca tgaacagctt ttcctttgcc gactttctga agacgccgca gggcgagacc
     6721 ctgcatgcgt gggagtcctc ccagtacgac gagttttcgg ccgacgcctt cggcagagag
     6781 gccctgcaag tcggcgcacc tcagctcgac acgctgagag cgaacggcat cacttcgcag
     6841 tggctcacga gtgaaagcta cgacgaactc gacaacaggc ttcttcgtcc caccctgcaa
     6901 accgtgcagg ccgaaagcgg atggctgccc tttcccgccg aatcgcttga tctggtgaca
     6961 cttcctcatg cgcttgactt cgcgccctcc gcacaccaga cacttcgcga agcggctcgc
     7021 gtgctagtgc ccgaaggcag gcttatcctg acggtcttca atcccctgag cctctggtgg
     7081 atgcgccaga aggccgtcgg cgcaggcctg aggccctacc tgccgacaca cacctcaccc
     7141 gttccgctct accgcctcaa ggactggctc acgcttctgg gattcgaaat cgaccgtggg
     7201 cgcttcggcg tctattcacc gagctgccat agcctcagga acttcgaacg ctggaaatgg
     7261 cttgacaagg cgggtgaccg ctgggcgccg cactgcgcca acctgatcgt gctctgcgcc
     7321 gtcaagcgcc tgccaggcat gaagctcgtc ggacgcctgc cgatgaagag tccgctcgac
     7381 atcctgacgg cgggctcgcc gctccccgtt cccaaggagg ccagacggca ggcgggacac
     7441 ggcaactgac ggtacactgt ctgctttctc aaccgttccg caacatcctc acatggcaca
     7501 gcaaaccgaa aagacactgg aaatctggac cgacggcgcc tgcaagtgca accccggcat
     7561 cggaggctgg ggcgcctaca tggtctgggg cgcgcatacc cgggaaatgt acggcggcag
     7621 caagctcacg accaacaacc agatggaact cacggccgtc atctcggcgc tctcggctct
     7681 gcgccgcaag tgcccgatca tcatccacac cgacagttcc tacgtgaagg acggcatcac
     7741 gaagtggatc cacggctgga agcgcaacgg ctggcgaacc gccgacaaga agccagtcaa
     7801 gaatgcggag ctctggcagc agcttgacga acttacctcg cagttcgaca tcgaatggcg
     7861 ctgggtgaag ggacacgcgg gcgacccggg caacgaaaag gcggacgagc ttgcggacat
     7921 gggctgcgaa gtcgcggccg gcacgcgccc cgctccgagc tattgagcac ccgtatacca
     7981 catttgcggt atttctttcc cagccactct tgcaaaaccc ggaaaaatcc gtataattca
     8041 aattctcgac gcgcggttag ctcagcggta gagcactgcc ttcacacggc aggggtcact
     8101 ggttcgaaac cagtatcgcg caccagaatt ccaaagccct tgaaaacgac gttttcaagg
     8161 gctttttcgc attctggtcc agcaagcgca aaaagcggac cctctcagga aggtccgctc
     8221 aaggcctgcg caccaacgga tgctgcgtca gcccgcactt ttcttgcggc agtccgcgca
     8281 aagtccgtag agcaccatcg tatggtcgct gagctcgtac ccatagcgct tggcgacggc
     8341 ggcctgctcc ttctcaatgg agggagcaac aaactccacc acctttccgc actgcaggca
     8401 gacgagatgg tcatgatgcc cgccgtcctc aagctcgtac gtggcccgat ccgtgtcaaa
     8461 atggtgtctg acgatgagac ccgcctgctc gaactgggcg agaacgcggt agacggtcgc
     8521 aagaccgatg tccactcctg tgctcaccag ctccttgtaa aggtcttcgg cggagatgtg
     8581 acggcgttca ccgttctttt cctcggcaca cttttgaaag agctcaagaa tcctgagcct
     8641 ggccgccgtg gccttgaggc cggcgttctt cagttccgag gtttggtttc cagacgtatc
     8701 gttcacgtta gtccgtccct tcaaaagttc atttacccgt tatcatacga ggttggtgta
     8761 acttctgcac accgcaacac cgctcactgg attttttatg cttgccaaca gattctctct
     8821 tgctgctctg gggatgactg cagcgctggt cctgaccggc tgcaatacgg tttccgaagc
     8881 ttgggacagc ccccgcagcg tcgtccctga attcctgcag ccctatcgtg ccgacgttca
     8941 tcagggcaac ctgatcacga gtgaaatggt tctgcagctt gaaaagggca tgacccagcc
     9001 gcaggtgcag ttcctccttg gcattccgct tctgcgcgac cagttccacc cctaccgttg
     9061 ggactacatc tactatctgc ttcgcggcaa cgacgaccgc cagttgcgca agctcaccgt
     9121 ctggttcaat gaagacggcc gcgtggacca ctggacatcc gacccgatgc ccgacgagga
     9181 gcaggccgac cagatgatcc tcggcacgac ggatacgttc gagccccgcg cccctcaggt
     9241 tgatcccggc gtcgccgccc agcactgatc cgttttcacg aggagcatca tcatgaatcc
     9301 tcagattgaa cgcctgcagg ccctcgtggc gcagctcatt gcgcgcctcg agcacgaaag
     9361 atcggaaagc aggcgtctgg cctcccaact cgcctcccgc gaggccgagc tcgcccagac
     9421 gaagcaccgc cttgaagaca tgcggcgcaa ggtcgaggcg ctcttcagcg cctcccgcag
     9481 cggcgatccg cggctctgaa ggagaacgtc atgcctgaag taaccgtcaa catctacagc
     9541 cgctcctacc gccttgccgt cagcacgggc gaggaggccc tcatccagcg ctgcgccgac
     9601 atcgtcgaca agcagatgaa ggccatccgg gaggggggaa aggtgatcaa tcaggatcag
     9661 atcgccgtgc tcgcggcgct tgaagtcgct tacgaggcca acaagaacgt ccagaagaac
     9721 tccacggacg cccagcgttc cgcagcggaa aaggacgccg agattgcccg tctcaaggcc
     9781 cgcatagcgg aacttgagtc ccgtcccgct cccgttgcgg cacccctgca ggcctcgggg
     9841 ccggacgcgg agaccacgga ggcaatcgag cgcctctgcc gccagtgcga gcaggccatc
     9901 tacgcaaaca tgagccgcgg cgccgtcctc tgacacccgc ttatcaaatt gcaacgacct
     9961 cccacgctgc agctcaaatc cgctacaatg aattcaaccc tgtagcggtg agcaccgggc
    10021 tccaatgttc ctagaaccaa tgctaaaccc attaggttgg cagactcgag aaccgatagt
    10081 gtgcagaaca cgcgttgtcc tgcccgttcc gctccggagc cgaccgccct gaacttccgg
    10141 ttcaggtaac gtagtccagc cccgctgcag gtcccttttt tcacgggcag tccttctcga
    10201 agggactgcc cgttttgcta ggcagatccc ccatgaacaa cacgaccgtt acggaagacc
    10261 tcctgcgcac cgccagtcgc ttctggctca tgaaaagcga acccggcgag tgctccatcg
    10321 acgacgcgct cgccgctccc ggccgcaggg tctcctggtt cggcatccga aactatcagg
    10381 ccaggaactt catgcgcgac gacatgacga tcggcgacgc tgtgctcttc taccactcga
    10441 gctgtgccaa gcctggcatt gcgggcatcg ccagaattgc ctcggacgtc taccctgacg
    10501 agctgcagtt cgatcctgaa agcgagtact acgaccccaa gagcacgaag gagaatcccc
    10561 gctggctcac gcttgatgtc gaagccctct tcaagtgccc cgtcgtcgaa atcggacgtc
    10621 ttcgggaaga gcccgagctg gcgggcatgc gagtgcttca gaagggcaat cgcctctcga
    10681 tcacgcccgt cgagaaggag gaatttgcct tcatcgtcag aaaccttctt tagcctgaag
    10741 gcatcctgct cgaactgagc atctcgagag atcacgaaga atcgccgcaa gttgttcacg
    10801 ctgattggcg cacccgtccc attcgttgga gctcgatcac cagaattatc gtgaacgtga
    10861 cattcacagt ccctcctgtg gcaatcctgt agcaagccgc ccgtattcca atggaatccg
    10921 ggcggctgct tcgttttcag acatggagga tgcaggtgcc cgttcaggcc ctgttccttc
    10981 ttcccgccca caccatcacg gcaatgccga ggacgatgag cgggagcgtg agccactgtc
    11041 ctctggaaag gtccagcgcc tgcagtccca agaaagcatc aggctctcgg aaccattcga
    11101 ccacaaagcg cgccacgccg tagcctgcac agaaaagccc cgacacaaag cctgcagccc
    11161 tcggtctgga ggaggcccac cagagcagga tgaaaagcag aagcccctct ccgagggctt
    11221 cgtagagctg cgaggggtga cgcggcacgc cgtcgcccgc ctgagggaag atcatcgccc
    11281 atggaagcga tgcatcagcg ggacgccccc agagctctcc gttgatgaag ttgccgatgc
    11341 gcccgagcat gaggccgagc ggcgcgagcg gtgcaacgaa gtccgaaatc gtccagaacg
    11401 aaattccatg cagacggccg tagagaatca tgacggcaat gacgccgaga agaccgccgt
    11461 gagcgctcat gcctccctcc cagacatgca ggatctgcat ggaatgcgac aggtagaact
    11521 caggctgata gaaaaggcag tagccgaggc gaccgcccag aatgacgccg aagatgccgt
    11581 agaaaagcag gttctccaga tcctcgcccg aaatcccgcg ccacggttcg gaacttctgc
    11641 gcctgccgag aagccagaag cagaagaagc ccgccagata catgaggccg taccagcgta
    11701 cggcaagcgg tcctaggctg aaggcaacgg gatcgaactg aggatgaatg agcatgggcg
    11761 tttgtttggc gaaatgttgt gaagggcatc cgcttgggga taagcaggta ttatgaagcc
    11821 ttcagaacct tttggcttag gccttttcag caaaatgacc gcacccctgc tttcgggcgt
    11881 cgacgccgcc cgccatctcg agcttctgcg cacggcccgc ccgctcgttc actgcctcac
    11941 gaacgaagtc gtgcaggaaa tcaccgccaa cgtcctgctc gccgcaggcg cctcgccggc
    12001 catggtggtc gccgaggagg aggcaggctt cttcgcgggc attgcaggag gcgtcctgat
    12061 caacatcggc acgccctacc catcacgtct gcgcgccatg catgcctcgg ctgatgccgc
    12121 acgtgccgca ggccgcccct gggtgctcga tcccgtcgcc gctggcggca ttccttggcg
    12181 cgacggcatc atccgcgaat tcgtcgagaa gcagccgacg gtcattcgcg gcaacgccag
    12241 cgaaatcctc gctcttgcgg gcgaaaagtc cggcggcaag ggcgtcgaca gcacggactc
    12301 gtccgacgca gccgtcgagg gtgccatgcg actcgccggc agttcgggct cgatcgtcgt
    12361 cgtcacgggc gaaacggact acgtcactga cggcgtccgt acgctacgcg tgacgggggg
    12421 cgaccccatg gccacgctcg tcgtcggcac gggctgctcg ctctcagcgc ttgtcgccgc
    12481 cttctgtgcc gtctccgatg accgcctgac cgcctcggcc acagcctgtg ctctggcgaa
    12541 gaaggccgcc gaagcagccc gccgaaggtc cgaaggcccc ggcagcttcc acaacgccta
    12601 cctcgacggg ctccatctaa tcaagcccgc cgacttcaca tccgccgaat gaggtacgtc
    12661 atgtccgaca actacagggt tcttcgcgcc cttcgtcttt atctggtgct tgatcccggt
    12721 ctctgcggcg gctccgaggg cttggtccgc acggcgcaag ccgctgccgc ggcaggcgtc
    12781 acggccgtgc agctcagggc tcccgactgg aagaagcgcc tttatgccga atgcggcagg
    12841 gagctcctca aggcgctcgc cccgtacgac gtgccgctct tcatcaacga ccacgcggac
    12901 gtcgccgtcg ccatcggcgc tcacggcgtg catgtcggcc agtcggatct ctcgcctgaa
    12961 gactgccatc gcctgatgac gcccggcatg atgctcgggc tttcggtctc caacgtggcg
    13021 gaatgcctcg gcgtggatcc tgcgctcgtc gactacatcg gcatcggtcc ctacagggct
    13081 acgcccacca agaaggatgc ggctgccgaa atcggtcttt cgggcctgac ggacatcgtc
    13141 agccgtgcgc cctgtccctc ggtcgccatc ggcggcatca aggctgccga cgtcggcagc
    13201 gtcatgcagt caggcgtcga cggcgtcgcc gtcgtctccg ccatctgcgg tacgcccgac
    13261 atccccgcct ccgtcaaggc gcttctcgac gccatgccgt cctgaccttt ccccacgtcc
    13321 gccgatcctc gagacgtggc atgatgactg cagaagcggg ccctttttcg gagcgttcct
    13381 ccggggcccg cactgtccct tgcctgagca gttccgacca tgtccgactc caccccaaaa
    13441 ttcgttcacc tctccgaaag cgatgcccgc gacgtccttt ttgccgaaat gctcgagaaa
    13501 agggcgggag gcgacggacg ctggagtgcc ggaaaccggg atgcggccac gcgcgacgcc
    13561 aaaaggctcg cgggcgaggg ggtaggcgca agagactttc tccccctgag agccaggctt
    13621 gtggccgccc gcctgcacga ggatcccgcc aatgcgttcg ggacaacgtc gtttcgccgc
    13681 gcaagcctcg cgctgaccgt cattctcggt cttgccgccc tgatgagcgg aatgctcacc
    13741 gaccacctcg ccaccgaggg tgcaaggatc aaccttctct cgccgcctct ccttcttctc
    13801 atcgtctgga accttgcggt ctacctgctg atcattctca agcccctgat gccgagcggc
    13861 acctcccgac ggcctctgtc cgtcagggag ttcctctcca gaagccttct gaaggccgca
    13921 ggctcctccg ccgtctccgt tgaagcagga actgcgctgt tcctgcccca atgccgctgg
    13981 cttgtcgccc gcgccctgca cattgcagcc atcctgttcg ccgcaggcct tctcgcaggc
    14041 atggccgtac gcggcatcgg caccgcctac cacgtcggct gggaaagcac ctggctcgcc
    14101 gacaaccccg aggccgtcca caccgtcctt aatacgattt acggtcatct tcctttctgc
    14161 ccgccagtgc ccgacgtcag cgcggtcgcc gcgctcgcct tcgacgcgca gaccgcctcc
    14221 gcagtcgacg ccgcaccatg gcttctgcgc ctaatgtgcc tgcttgcact cgcaatcatt
    14281 cttccccgcc tgttgctcgt cctgaagtgc accgtcgggc tcgcagcaag ccgtcgtcgc
    14341 gtccgcatcg accttgagtc tccctactac cgttccattc tctcggtatc cgacgctccc
    14401 gctgtcagaa caaccgtcat cgtcgacagc acggattcgg tcgacggcct tcccgcacac
    14461 tggcatgact tcaaggcaaa gctcaagact gccgagcccg gctcaggaga cgtcgacctg
    14521 ctggccaaca gtgcctggga cggcacaacg agtgaagttc tcgccgccgt caaacccgcg
    14581 atcatccacc gcctgcttct tgccttcgac ccgtcctcca ctccagaagt ggaggtgcac
    14641 ggggccatga tggatgcggc agcctcttgg tgcacctcga agcagagtcc cgcccccgtc
    14701 gtcgtgcttg atctttcacg catcaacagg cgcttcggca gggaaaaccc cgccgcaggc
    14761 agtcgcacgg ccctctggca aacctttgcc gcagagcggg gcctcagaac gctggccacc
    14821 gacatgagca ttcctgaaga cgtcgaccga atctgcacgc tcctttcagg tcaggtgcga
    14881 taattcagga acagtccgaa aacgcgtaga agaaaacatg tccgatcacg atcagttccg
    14941 cattcacctc agcctcgtga gccacaccaa catcggcaag accacgcttg cccgcaccct
    15001 gctgatgcgg gacgtgggcg aggtcgccga ccgcgcccac gtgacggaga cgacagacga
    15061 ctacgtgctc gcccgcgatt cggcaggctg cgagcttgtt ctctgggaca ccccgggatt
    15121 cggcaactcc gtcgcgctcg ccaagcgcct tgcaggtcga agcaacccga tcggatggtt
    15181 cctctccgaa atctgggacc gcatgacaaa caaggccttc tggctcaacc agagggccat
    15241 caagcacgtg cgcgacatct ccagcgtcgt gctctacctc gtcaacgcct cggacctgcc
    15301 caagacagcc ccatacatca cggcggagat gcagatccta tcctggatag acaaacccgt
    15361 catcgtgctg ctcaaccaaa tgggcaagcc cagaacgcat gctgaggaac aggcagacgt
    15421 cgccgcttgg cgagaggcca tggctccgta ccccttcgtg aaggacatcc ttccaatgga
    15481 cgccttcgcc cgctgctggg tgcaggaaac ggcgctgttc gattcgatcg gcagggcgct
    15541 gcccgctcag atgcactcga cgttcgacac gcttcgggac atctggacac gcagccgccg
    15601 cgccctctac ctctcctccg tcgacgccat ggcccgccac atgtggcgtc tgcttcaggc
    15661 ccacgaactc gtcccgactc ccacgctcaa ggaccatctg cgctccttcg gctcacagat
    15721 cggcctcatc aagacgcgca acgaggcgct tgaggacgcc cagaccgcac tctcggccat
    15781 ggccgccgac agcctgtgct cgctcacgga caagctcatc ggaatcaacg gtctcacggg
    15841 aagcggcgtt tctcgcgaaa tcctgcgccg catgaagacc gactggcaga tggcctcgca
    15901 cagcatctcc cccggccccg ccgccgctgt cggcgctagc gtgggcgtag cgggcggcgc
    15961 tgcagcggga gccgcagccg acatggcgag cgcaggactc tcgctcggac tcggcacgct
    16021 gctcggcggc atcatcggtg ccatcggcgg cgtcggcgcg gccaccatct acaactcccg
    16081 ccaccgcaag gagggcatcg acatctcctg gagcgaacgt gccgctcagg acttcatgct
    16141 ggaaagcctg ctcctttatc tggccgtcgc ccactttggg cgcggccgcg gcgactggca
    16201 ggaaggcgaa agccccgaat tctggaagaa ggccgtgcag gacgccatga ccgaacagaa
    16261 ggtgagcttt caggccatcc gcaagctcac gcccgaagag ggtgtcgaac agctcacccg
    16321 catgacggac tccctgctga ggggcgtctt catgcaactc tatgaacgga gcatctgacc
    16381 gacatgctct ccaaaggctt tgcaaccatc gggcttctgc tcgtctcgaa cgtcttcatg
    16441 acgctcgcct ggtacgccca tctcaagctc cagagcgcca aactcatcag caactggccg
    16501 ctctacaccg tcgtgctctt ttcctgggcg atcgcgcttc ttgaatacag cttcatggtg
    16561 cccgccaaca gaatcggctt cgagggcaac ggcggtccct tctcgctcat tcagctcaag
    16621 atcattcagg aggtcatcac cctgacagtc ttcacgctct tctccgtcat cgcctttcag
    16681 cagcatctgg cctggaacca cttcgccgcc ttcgcctgtc tggtggcggc cgtctatttc
    16741 gtcttcatga agtaagggct cagatcggca tctcatcaac gccataacga acaatgccgc
    16801 ccgaacacga agcccggacg gcattgttca ttttgaggtg caggatgcag caaccttacg
    16861 gcaccatttc ctcaacctcg tccttcttga ccggacgcac gaggtcctcg cgcttcacgc
    16921 caagatagag cgcaatggcc gccgccacga agacggagga gtagacgccg agcagaatgc
    16981 cgatcgtcag ggcgagcgag aagtagtgca gcgccggacc gccgaagaag aacatcgaga
    17041 gcgtcatgca gagcgtcgac gtatgcgtga tcacggtacg cgaaatcgtc gccgtaatgg
    17101 ccgagttgat gacctccatc gtgtcggcgc gacgcatcgt gcggaagtgc tcgcggacgc
    17161 ggtcgaaaat gatcaccgac tcgttcacgg agtagccgag gaccgccagc accgcagcga
    17221 gcacgggaag cgagaactcc cactgcatca ccgagaagat gcccagcacg atgaagatgt
    17281 cgtgcaggtt ggcgatgatg gccgccaccg agaacttcca ttcgaatcgg aaggcaaggt
    17341 agatcatgat gccagccacg acgagcgcaa gcgccgtgcc gccgtcgcgg gcaagctcct
    17401 cgcccacctg gggaccgacg tattcggtac gggtgagctg ggcttcaggg gtcttctggt
    17461 gaatgacctt catgacctcc tcggccacct ggcccgaggt gtagccctgc ttggtcggca
    17521 cgcggatcac gatgtcggag gccgtgccga aggtctggac gagcacttcg ttggagacgg
    17581 ccttgaggtc ctgtcgaacc tgttcggtgt tggccgcgga cggatagcgg acctccatct
    17641 gcgtgccgcc ggtgaactcg atcgagaacg caaggccgtg cgtaaagatc cagaaaacgg
    17701 cgacgatgaa gctcaccaga gagatcagat tcagcacatg cctgtagtgc atgaacggaa
    17761 tcgtcttgtg aatgcggaaa aattccatga tgttatcccc tcatcagcga gcagtcttct
    17821 ctgcgtccgg acgccagatc tgaccgatgt gaaccttcgt cagcttcttc tggcggccgt
    17881 agatcagatt gatcatcgag cgggagacca cgaccgacgt gaagatcgag gtcatgatgc
    17941 ccagacagtg caccacggca aagccgcgga cgggaccgga accgaagatc agaagcgcca
    18001 gaccggcgat gagactcgtg atgttcgagt caagaatcgt cgcaaaggca cgatcgtagc
    18061 cttcgctgat ggcggtctga ggcagacgac cgagacgcag ttcctcgcgg atgcgttcgt
    18121 tgatcagcac gttcgagtca acggccatgc cgagcgtcag cgcgatggcg gcgatgccgg
    18181 gcagcgtcag cgtagcctga agcatggaca ggagcgcgat gagcatcatc acgttgcaca
    18241 cgagcgaagc ggcgctcaca agaccaaaga cctgatagta gacggccatg aagagcgcga
    18301 tcacgatgaa gccgtagagc gtggacttga agcccgcgga aatgttggct tcgccgagcg
    18361 acggaccgat caggcgttct tcgacgattt ccatcggagc agccagcgag ccggcgcgca
    18421 gcaacagggc cgtgtcggtc gcctcgaccg tcgacatgcg accggagatc tgcacgcggc
    18481 cgccgccgat ttcctgacgg acgacgggag ccgtcacgac ttcgcccttg cccttttcaa
    18541 agaggatgat ggcgattcgc ttgccgacgt tgtcgcgggt cacgtcctgg aagatgcggg
    18601 cgccgcggct gtcaagctcg aggttgacgg tcggctcctg cgtctggctg tcaaagccgg
    18661 gctgggcgtc gttgaggttt tcacccgtga ggatcacgcg gcgcttgacg agaagcacgt
    18721 tgccctcgcg gtccatgaac ttctcatcgc caaagggcac gttgccctgt gcaagctggg
    18781 cgagcgcctc aggagagtcg tcgacaagac ggacttcaag cgtggcggtg cggccgagaa
    18841 tgtccttggc cttggccgta tcctgcacgc cgggaagctg aaccacgatg cggtccgcac
    18901 cctgctgggc gatcactggc tcggcaacgc cgagttcgtt gattcggttg tgcagggtcg
    18961 agatgttctg cttcagggcg taggactgga cgttctggac ggccttctgg gagagttcgg
    19021 cgcgcaggag gtacggagcg gcggaagcct cggggagcga gagcaccaga tcaggctgcg
    19081 tgttgcgcag caggtcattg gcgcgggcgc gttcctcttc ggaagcgaag ctcacttcaa
    19141 ccacgttgcc cacgcggttc atgcccgtgt ggcggatgcg cttgtcgcga agctgcgtgc
    19201 gaaggtcggc gagcgtcgct tcggcgcgct tcgtaatggc cgcgcgcatg tcgacctgaa
    19261 gcaggaagtg cacgccgccg cgaaggtcaa ggccgagata catcggcagt gccctgatcg
    19321 aaagcagcca ctgcggggtg ttcggggtga ggttcagggc caccacgtag gcggggtcct
    19381 cacggttcgg gttgagcgcc ttttcaagga cctcgcgggc ctgaagctgc ttttcggcct
    19441 cagtcggctc gaagcgtacg cgcacggtgt tctgctgggc gccctgttcg aagaagatgc
    19501 cgttgggatg caggtctgca ttgcgcagaa gatcctcgac cgtcttcagg gtctgctccg
    19561 tcaccttcac cgtcgccttg ccggacgaga cctgcactgc aggagactcg ccgtagaagt
    19621 tcggcagggt gtagaccaga ccgatcacaa gggcgacaac gatgacgatg tatttccaga
    19681 gaggatagcg gttcatcgca tgttactccg ttgcgccgca ggatttttcc tcaggcgcaa
    19741 ccgttgtgaa aaccacttga agaaagtggg gttcgaaaat gggcttaagc ccggacgctc
    19801 gtccgggctt aaaaaagcgc caagagcatc tgctctcagc gcttcgtgca cttcagtgcg
    19861 tgcacgttga tgctgctcag atcttcagcg tgcccttcgg gaggacgacg ctgaccgagg
    19921 tgcgctgcat gcggatcacg acgggcttgc cgtcgacgga agccacctga aggccgatcg
    19981 tctgttcatc gacggactcg atgcggccgg aaatgccgcc gagcgtggtg acttcatcgc
    20041 cgcgcgtcag gccttcgcac atctgcatgt gttccttctg gcgcttctgc tgaggacgga
    20101 tcaggaagaa ccagaacacg gcaaagatga cgacgagcgg aacgacctgc atgaggaagc
    20161 cttccatgcc gcccacctga gcggcggcct cgccggcagc catggcatcg ctaacaaaaa
    20221 acatttgaac tccagtgatt caggaatgtg tgaaatcgga accttcggcg gaagcgagtc
    20281 ccgccgaagg agatttgaga cattataagg atcttgcctt atctctgact gagaaactcc
    20341 gggaggattt actccacccc gcgggcgcga tcctcggcga actgcttctt ccaggcctcg
    20401 aacgtccctg cctcaagcga gtcgcgcatc tcctgcatga tctgaaggta gaaggagagg
    20461 ttgtggatcg tgttgaggcg cgcgccgagg atctcgccta ccttgtggag atgatgcaga
    20521 taggcgcgcg tgaagttgcg acacgtgtag cagttgcagc tcggatcaag cggacgcaga
    20581 tcgttgcggt aacggctgtt cttgagcttc acgtcgccga aacgggtgaa gagccagccg
    20641 ttgcgcgcat tgcgggtggg catcacgcag tcgaacatgt cgatgccctg ggagacgccg
    20701 gccacgaggt cctcgggcgt gcccacgccc atcaagtagc gcggacggtc gtcggggagc
    20761 ttgtgcacga tgtggtccat gatgcggagc atgtcctcct tgggctcgcc gacggaaagg
    20821 ccgcccacgg catagccgtg gaagccgatg tccttgaggc cctcgagcga ctcctcacgc
    20881 aggtgctcga acatgccgcc ctggacgatg ccgaagagtg cgttcgggtt ctcgagacga
    20941 tcgaactcgt cacgcgagcg cttcgcccag cgaaggctca tgcgcatcga cttggcggcc
    21001 tcgagctcgg tggcgggacg gtcgccgatc ttgtagggcg tgcactcgtc gagcaccatc
    21061 accacgtcgg agttgagcac gcgctggatc ttcatcgaca cttcgggcga caggaagagc
    21121 ttgtcgccgt tgatcggatt gctgaacttc acgccttcct cggtgatctt cctcagttcg
    21181 ccaaggctga agacctgaaa gccgcccgag tcggtcagga tgggcttgtc ccactgcatg
    21241 aagtcgtgaa gaccgttgtg ggccccaatc acctcgagcc cgggacgcag ccacaggtgg
    21301 aaggtgttgc cgaggatgat ctgggagccg atctccttga gttcataggg cgccatcgcc
    21361 ttcacggaac cgtaggtgcc gaccggcatg aagatcggcg tctgaacgac gccgtgattg
    21421 agcgtcagtt cgccgcgacg ggctctgccg cacgtcgcct tgaccttgaa atcaaaagcc
    21481 atgtttctct tgtgtccttg tcttgtgtac aggtcctgct ggccggatca gcctgcaaga
    21541 cgagcgtttt cgatgaagca tgcgtcgccg tagctgaaga agcggtactt ctcctcaacg
    21601 gcatggcggt aggcctccag aatgcgctcg cggcccgcaa gggcggagac cagcatgacg
    21661 agcgtcgact tcggaagatg gaagttcgtg atgagcgcgt cgatgatgcg gaagcgatag
    21721 ccgggcatga tgaagagcga ggtgtcgcgg gcgcccgcgg ccacgtgccc gacggcgtcg
    21781 gccgcgctct cgatcgtgcg cagcgacgtc gtgccgacgg cgatcacgcg gcggccctcg
    21841 gccttcgcac gattgatgcg cgcggccacg tcctcgggca tgtt
//
LOCUS       DBMDTI010000009        21482 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_827_length_21482_cov_3.137692, whole genome shotgun sequence.
ACCESSION   DBMDTI010000009 DBMDTI010000000
VERSION     DBMDTI010000009.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 21482)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21482)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21482
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_827_length_21482_cov_3.137692"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(342..866)
                     /gene="def"
                     /locus_tag="ACZPB6_01325"
     CDS             complement(342..866)
                     /gene="def"
                     /locus_tag="ACZPB6_01325"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR1183150.1"
                     /translation="MIRPVLKMGEELLLEKAMPVKAFGTPELRRLVVDMWDTMDVEGG
                     IGIAAPQVGVSLQVICFGTKESEEASGARRVPRTVLINPVVEPVGEETMSAWEGCLSV
                     PGLRGRVVRPVAVRYKGFDLEGNRIERTVDGLHARVVQHEADHLVGILYPMRVTDWRT
                     FGYSEVLFPEKSKA"
     gene            complement(863..1627)
                     /locus_tag="ACZPB6_01330"
     CDS             complement(863..1627)
                     /locus_tag="ACZPB6_01330"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HIR1183151.1"
                     /translation="MLALIGGSGFTGNDVIEDCEEIEITTPWGAPSAPLVFGRLGGRS
                     IVFLRRHGVKHEFAPHRVPYRANIWALQKAGVEGVIAVGTVGGVAPDMGPGRIAVPDQ
                     LLDYTWGREVTYYDDFGTAGMKHVDMTWPFDRALSARLVEAAERLGVGVRTAGVYACT
                     QGPRLETAAEVRRMCRDGADMIGMTLYPECALARELDLPYAGICVSVNHAAGMGDCAD
                     EIDFASLESSLARAVRGVVDIVRETVRADAQRGGAE"
     gene            complement(1826..4141)
                     /gene="ligA"
                     /locus_tag="ACZPB6_01335"
     CDS             complement(1826..4141)
                     /gene="ligA"
                     /locus_tag="ACZPB6_01335"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR1183152.1"
                     /translation="MTEPTKTETLANDDFDGAAKAAAIEPSRAPARMRELEKLVETWD
                     YEYFVLDNPSVPDAEYDRTFNELKALEARFPEWKSPASPTARVGGAVRSDLAKVVHAV
                     PMLSIHTETDFSSEGAAAFDRRVRSELGLADEDPAVAYDCELKFDGLATNLRYEKGVL
                     VQAATRGDGSAGEDVTANARTIRTIPLRLEGDFPDVLEVRGEVIMHRDDFEALNRRQA
                     EEGQKTFVNPRNAAAGSLRQLDPAVTARRTLHFYAYALGEVSGGEFAPTQTEVLDRLA
                     SLGFPVADVRRRVEGPTALATFHDEVAEIRHTLPFDIDGVVYKVDSLELQRELGFIAR
                     EPRWACAHKYPPEEALTVCEAIDIQVGRTGKLTPVARLKPVFVGGVTVSNATLHNEDH
                     IAGLDLRPGDTVVVRRAGDVIPEVVRVVHERRPEGTVPFAMPSVCPVCGSATIRDEEE
                     KDTRCTGGLYCPSQMKLSLVHFASRRAMGIDGLGEKIVNMLVDEGLVKSPADIFTLKP
                     EDLIAPTTATKDLEKPVKRMGPKAASNLLESIGKAKHTTFARFIFALGCRHVGEATAL
                     SLAQHFCTLANLEAADVEALTKVDDVGEIIAESIHAFLKEPHNRTVIEALVASGVVWP
                     VVEPRPAAEASEVAGKTFVLTGTLPTLTRDEAKDRLIAAGAKVSGSVSKKTDFVVAGA
                     EAGSKLEKAQTLGVKVIDEETLLAMLEGGVSSEKLVVTPEAEEEEKAAAEGAVSTQDA
                     SDTPANPEEKRAAGTEVGKTDDEGPQQGALF"
     gene            complement(4240..5415)
                     /locus_tag="ACZPB6_01340"
     CDS             complement(4240..5415)
                     /locus_tag="ACZPB6_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183153.1"
                     /translation="MSQTQIILIVAALVVIAFFFWRSMREERRMRASRKARLQAGGDV
                     RKDVLAEEKPEPRVEPQLEDMVDDDEPHEAVQSASRDDLPSEKPPVYERFAEEQKARL
                     EREAARGGRKAGRIAGIEKPPVDHGIQWVLDVTPYEGKTFPLGSMDSLVTQIRQLSLP
                     LPVSLWAKSAEDGLYYPSECLPAEAVHIVATILIANRAAVLDDVSASSFLQVLEQTAA
                     QNDVDVRTSCDIKTAVSTAVQISRFIHYYDKLIEVSIVPAPREEGLTLEEVTKVAEGA
                     GFVSASGRWELRIDEGSREPLMTLSLAGADSSQLRLSFDVPLANVPRGDLKRFFQLAN
                     HIACHLGGVWVDGAGNRIEAAGGLILQDEIEHQDAQMAASGVRPGSERARLLFSRSA"
     gene            complement(5527..9054)
                     /gene="smc"
                     /locus_tag="ACZPB6_01345"
     CDS             complement(5527..9054)
                     /gene="smc"
                     /locus_tag="ACZPB6_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR1183154.1"
                     /translation="MRLRQIKLAGFKSFADPALIELEHPVVGIVGPNGCGKSNIIDAV
                     RWVLGEARISELRGSSSMRELIFAGSTTRHALGRASVELILTNDDGRLKGAWSEFGEI
                     SVKRVVTAEGQSAYYINHQQVRRRDVQEIFLGTGLGPRSYAIISQGMISNFIRAKPEE
                     MRVYLEEAAGVSLYRERRRETESLLSQTRGNLERVRDMQAVKEEEAARLEGEAQLAGK
                     WRALTDERNAAESLWYWIQYEDVKRTLDGIAADIARGEAEIEARKADAAREAASLPAL
                     ESAAREAEAKELEAAAGLRQAERALTKAEAEERRQRERRAGAERAAAEAREAKAAKER
                     RLAETVSAREAQASETDELEETIALSGEEEAAAQEEVLSAEDALNDVNARAAEARRAE
                     SDAKAQARVAHARLEAAERRAEDLRQRLLRLAASEGTLEAPDKEGLEAMKLDFEEAQA
                     AAEEARALEEEAAEAAAEARRRRDAANEAYFAALAQEKETAAKLDALEAVEAGAQAKG
                     RLAEFEAAEGLDGLPRLSEALSADPEWVTAVEAVVGSRLGARFLSRLGMAQGYESRRP
                     PAAISFVERGETQEGRPALELDGVRLEPITSHVRCRSSDATAALADVAANVYCVKSLS
                     EGLALRARLPEGVTLVTPKGDRLTSRSLVVWTASDPGQSVLSRQQEISALRDCLHELE
                     DRLGKLDDDRNAAGAAAEAAERTARTSAQGARAAEAGVVDLRLRIKAAEAAVAAHETR
                     LADLRRSREELEAEGEEARALAEEAGVAADEAEAASERAVRAAAGFTSELARADMRFK
                     TKTAALAAVRHKAELARAKLRQLSESARLYAQSEAVLRSDIAREEARLVEASRLLEES
                     AKVLGDAASAAALKVLEEAERAHAARLDATAAARLALTEAQGRWQAAQADVMPMTEAL
                     GVKRVEQQMKESLRTQFEERLNELKADRTDLARRAAERPQKAQSLRAKVLRLISEIAG
                     LGPVNHAALEHLEAVRRTLEATARQVEDLEKGIETLEAAIRKIDAETRGRLRETFEEV
                     NGHFAETFSELFGGGVASLVMSGDDVLNAGVEVKAQPPGKKNAGVKLLSGGEQALAAT
                     ALVFAIFRLNPAPFCLLDEVDAPLDEANQARLAGLCRRMSSETQFLMITHHRVTMEFA
                     GALVGVTMKEPGVSRVVSVDIENAVRMAN"
     gene            9330..10256
                     /gene="prmB"
                     /locus_tag="ACZPB6_01350"
     CDS             9330..10256
                     /gene="prmB"
                     /locus_tag="ACZPB6_01350"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR1183155.1"
                     /translation="MYEIAPLLDDPIHNLKTIRDVFRWAITEMENAEISYGHGSPDSW
                     EEASFLICRGLKLPFDHFDMFLDAALTHNELSRLVHLIDRRVHEKVPTAYLLREAWLT
                     EHRFYIDERALIPRSYIAELLEEDLAPWVEDPYEVGSVLDLCTGSGCLAILAQGTFPN
                     AEVTGSDLSPDALEVAQINRRDYGMEETLELVQGDLFENLQGRTFDIIISNPPYVTQA
                     SMDTLPSEYRHEPAMALGAGDDGMDVVRRMLPEAAAHLNDGGMIIVEVGDGAEAVEAT
                     FPGLEIIWLATSGGDDQVFLVTKEALLNYFEQ"
     gene            10266..12194
                     /locus_tag="ACZPB6_01355"
     CDS             10266..12194
                     /locus_tag="ACZPB6_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1183156.1"
                     /translation="MLRLLNLALSRGTRVLYRHATLTAAPGERIGLVGPNGCGKSTLF
                     AAVLGDLSPEDGDIETPPRDRIAHVAQSFVADNVPAVDFVLGGHAPLIAARAALAEAE
                     KAGDDMRLAQAHANLAELNEGAIVAQARTILAGLGFSQTDVDRPVYDFSGGWRNRIAL
                     ARALMCPADLLLLDEPTNHLDIDSLIWLENWLRRVEATVVIISHDREFLDRAVTTIWS
                     VEDDTVCRYAGNYSAFESQRIEKLRLQDAAAKAYEREASHLSSFIERFRAKATKARQA
                     QSRIKMLEKLKAVEPVRAKREWRFEFPKPVRLPEHLVDTENLRLGYDERVVIDRVTLS
                     IRSGERIGILGVNGAGKSTLVKGIVGDLAPMAGELRRGQGLEIGYFAQHQLDQLRPDE
                     SPLEHMRRMAPDVREQDLRDYLGIYRFSGDFASAPVGPMSGGEKARLALALLAWRKPN
                     LLVLDEPTNHLDMETREALTMALSTYEGAVLIVSHDRHLLRATTERLILVHDGAVQPY
                     DGDLDDYAQLVLEHRRTTVAAEKAANQAERLESGEREPSQNRKEARRAEAQERQRIAA
                     LRKPLQKELAKVEKELAQKNEELRKLSDRLADGDFYTSTDPDEVARVLREHGELKPVV
                     EELEMRWLELSEEIEAVC"
     gene            12429..14621
                     /gene="speF"
                     /locus_tag="ACZPB6_01360"
     CDS             12429..14621
                     /gene="speF"
                     /locus_tag="ACZPB6_01360"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HIR1183157.1"
                     /translation="MRRYNIAATPGTLHAFKSKRVVVDLNKADLTSVSAVVMTADEAL
                     SGQLERVEATAFGIPVILLNNGAKLPTELFGKVVSVINSQPDNVGLYGRLIDAAAQKY
                     EDQILPPFFGKMESYVRNGNVHFDCPGHQGGAFFRRHPAGRQFADFFGENVFRSDLCN
                     ADVAMGDLLIHEGVPRAAQMAAAKIYNADKTYFVLNGTSASNKVVLNALLTPGDLVLF
                     DRNNHKSNHHGALIQAGATPVYLETARNSYGFIGGIDDHCFDEKYLRELIREVAPERA
                     DEKRPFRLAVIQLGTYDGTIYNARQVVDRIGRLCDYILFDSAWVGYEQFIPMMKDCSP
                     LLLDLGPEDPGIFVTQSVHKQQAGFSQTSQIHKKDLHIKGQKRYCTHKRLNNAFMMHA
                     STSPFYPLFAALDVNAKMHEGESGRRLWADCVRVAIDARKEMLACCHYIKPFIPPTVD
                     GKAWDSYPTEQIATDIRFFAFNPKDKWHHFEGYAPQQYFVDPCKFLLTTPGINATTGE
                     YSDFGIPASILASFLRENGVVPEKSDLNSILFLMTPAEDHAKMAHLITQIARFESFVD
                     DDAPLSEVLPELYNAHKERYKGYTIRELCQEMHDFYKSVNVKGLQKAMFRKEYFPRRV
                     LNAQEANYEFIRDNVELVRLSEAEGRVGVEGALPYPPGVLCLVPGEVWGGAVLQYFLA
                     LEQGINLFPGFAPELQGVYIEEDEDGRQVAWANVLTHERETELLGKAL"
     gene            14858..15676
                     /locus_tag="ACZPB6_01365"
     CDS             14858..15676
                     /locus_tag="ACZPB6_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1183158.1"
                     /translation="MQKRTFIKSLAAVTALSAIVLLTGCDEEKAVAEKPFRVGVTAGP
                     HAAIVTEAAKVAAKNGLKVEVIEFTDYVTPDTALNDGALDAAVYQHEPFLLNFNKQKG
                     TKLANVGNAVVQPMGFYSNKVKSIADIPEGAVISVPNDPTNCGRGLLLLQAAGLIKLP
                     EGMGSEASLADIVENKRNLKIRELEAAQLPRSLDDALVAVIPMNYVISAGLSPQKQGF
                     YFESLEAPFALIIIAAHQDRRNDKSVQDFVKYYRSPEVKAFVEKTFNGTIKPSW"
     gene            15986..17368
                     /gene="dcuC"
                     /locus_tag="ACZPB6_01370"
     CDS             15986..17368
                     /gene="dcuC"
                     /locus_tag="ACZPB6_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HIR1183159.1"
                     /translation="MLGILITVAVIAVVAYCVLKNYYPPIVLLLAGLLMLVCAWLMGV
                     DPVDAKKSTNFVGFDFVQAFTNQMKGRLPGLGLNIMLIAGFSFYMDRIGASKALVKLC
                     VKPLSAVRSPYVLLAITYVVGQFMALFINSAVGLGLLLMASVYPLLVALGVSRASAAA
                     VIGSTCCLDLGPSSSNAMRAADLMSTDVVTYFVQSQIPVAVCVIATVAIGHFFVQRWF
                     DQRDAARGVADGAPVKADQKDLEKAFEGAGPVHYALLPMLPLVLLIVFSPMVYDGIKL
                     SLQTGILVSILIAFFVDLITHFDFKECCKRTSAVFEGMGKVFTSTVALICCAELFALG
                     MNKMGGITTMIQAAAAMESAGVWVMLFIMLGIMVVATIVTGSGNAAFFAFSPLLPEAA
                     ASVGINTAVLVVPVQLSAGIARTMCPIAGVIIAVAGIAGLTPFEIIRRTIPVMLLALI
                     VNVAASAVFL"
     gene            17405..18550
                     /gene="iadA"
                     /locus_tag="ACZPB6_01375"
     CDS             17405..18550
                     /gene="iadA"
                     /locus_tag="ACZPB6_01375"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HIR1183160.1"
                     /translation="MAILIKNARVFAPKDLGVVDVLMANERILAVGKDLAPNLPDLQT
                     VEAGGMIMTPGFFDQHIHVTGGGGEGGPATRTPELVLSELVACGTTDVVGVSGTDYTT
                     RSIPNLLAKVRALQAEGVSAWMYTSNYRCPPTLLTDSIGNDLFFIPEVLGVKIALGDH
                     RSSFPDVQTVLSMLADIRVGAMLAGKIGFLHIHNGNIPGAFAMYEEIVSRGFPVKHIR
                     PTHCGRIRHVFDSAVQFALKGGWIDITTGASCCFDHPAQAVVEAIAAGVDPTHITLST
                     DGHGSVPRFNDKGEMVGLGVGGVDGNLKETIRLIRDHNMPIEQALTFTTSNVASALGI
                     KGHGVIETGACANACLFTEDFELKGVFARGREMMRDGEILVKGTFEY"
     gene            18741..19058
                     /locus_tag="ACZPB6_01380"
     CDS             18741..19058
                     /locus_tag="ACZPB6_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183161.1"
                     /translation="MDIELEWLQATLNDASEALQDALQKVENGDQEDALEVLNHDLVH
                     VYAKLNYAVNTARLGPAALDAMSEDELIGWPARMPFLTFDEIDTLGEDEEDEEAVEEE
                     DVK"
     gene            complement(19153..20697)
                     /gene="murJ"
                     /locus_tag="ACZPB6_01385"
     CDS             complement(19153..20697)
                     /gene="murJ"
                     /locus_tag="ACZPB6_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR1183162.1"
                     /translation="MSLLKSALTVSGLTLVSRVTGVIRDMLIARFFGASAETDAFYVA
                     FRLPNMLRRLFAEGAFQQAFVPMLADVKERSEGEAGTFIDHVFTVLALSVFAASILGV
                     VAAPLLVWAIASGLASAPEAFDLASALTRFMFPYIAFMALTALAASVLNTWKRFAVPA
                     VTPVLLNLSFIGCTVLLAPRMAEPIWALAVAVILGGVLQLGTQYLALCRLGIRVRPRG
                     LRDSLADGDVRRVLKLMVPALFGVGVAQLSILINTNIASHLGKGAVTWLNYADRLMEF
                     PTALLGVALGTVLLPGLSSAYAKGDTSRYNSLLDRGLRLVVLVAVPAAVGLWLTSEAL
                     VSFLFQGRSFTPDDVSQTALAVVGYSVGLIGLIALKIVAPAFYARKDIRTPVKTAFWS
                     LVVVQLVNLVSVPLFSHAGLALSVGVGSVFNAGTLLIILMRRGIYTPLPGWAKAVLRI
                     ALATLLMGAGLWYGQTFVSWTTMPWLMRAGGVLAMVAVAAVAYFAVMFACGWRLSDLR
                     PGRGDD"
     gene            21062..21325
                     /gene="rpsT"
                     /locus_tag="ACZPB6_01390"
     CDS             21062..21325
                     /gene="rpsT"
                     /locus_tag="ACZPB6_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR1183163.1"
                     /translation="MANTKQARKRARQMVVRNMHLSAQRSQFRTAIKAVRKLIVAGDK
                     AAAQEAFKKAESVIDSMARKGVLHTNAAARHKSRLSASIKAMA"
BASE COUNT         3954 a         7479 c         6201 g         3848 t
ORIGIN      
        1 tcaatctctg tttgtcgctc ttcgccggaa ttgaaagaga aggaattaac cttcgtcttt
       61 cggcttttcc ttcaaagcgc gaacttcgta agttttttga ctgccttaaa agtcaccctt
      121 gaaactgccg atgttcacac ttatcggttc gttgaattct gatttttaaa gaacagcgct
      181 cctaagagca cagagttgag agtttaccaa ctcatcaact ctttgtcaaa tttattcgct
      241 gatgacttgg cgtcgtcagc gaaggattga aattaaacac ctttccgcag gctttgtcaa
      301 gacttgtcgt aaaaataccg caaacacggt atccctccgg ctcacgcctt ggatttttcg
      361 ggaaagagaa cctccgagta cccgaaggtc cgccagtccg ttacgcgcat cggatagaga
      421 atgccgacca gatgatcggc ctcgtgctgg acgactctgg cgtgcagccc gtcgaccgtt
      481 cgctcgatcc tgttgccctc aaggtcaaac cccttgtatc ggaccgcaac cggcctcaca
      541 accctgccgc gaagccccgg caccgagagg caaccttccc aggcgctcat cgtctcttcg
      601 ccgacgggtt cgacaacggg attgatgagc acggttctcg gaaccctgcg tgcgcccgaa
      661 gcctcctcgc tctccttcgt accgaagcag atgacctgaa gcgagacgcc cacctgaggt
      721 gcggcaatgc cgatgccgcc ctcgacgtcc atggtgtccc acatgtcgac cacaagcctc
      781 cggagctcgg gcgtaccgaa cgccttcaca ggcatcgcct tctcaagaag caactcctcg
      841 cccatcttca acacgggacg gatcattcgg cccctcccct ctgcgcatca gcacgcacgg
      901 tctccctgac gatgtcgacg acgcccctga cggcacgcgc aagcgacgac tcaagcgacg
      961 cgaagtcgat ctcatcggca cagtcaccca tgcctgcggc atggttgacg gaaacacaga
     1021 tgcccgcata aggaagatca agctccctgg caagggcaca ctcgggataa agcgtcatgc
     1081 cgatcatgtc ggctccgtcc ctgcacatgc gcctcacctc ggccgccgtc tccagtctcg
     1141 gaccctgagt gcacgcatag acgcccgcag tcctcacgcc cacaccgagc ctttccgcgg
     1201 cctcaaccag tctggcgctc aacgccctgt cgaacggcca cgtcatgtcg acgtgcttca
     1261 tgccggcagt cccgaagtca tcgtaataag tgacttcacg gccccaggtg tagtcaagca
     1321 gctgatcggg gaccgcaatg cggccgggcc ccatgtccgg cgcaacaccg cccacggtcc
     1381 cgacggcgat cacaccctcc acgcccgcct tctgcagcgc ccagatgttt gcacggtagg
     1441 gcaccctgtg cggggcgaac tcatgcttca cgccgtgacg acgcaggaag acgatcgacc
     1501 ttccgccgag gcgtccgaag acaagcggtg cgctcggagc cccccagggc gttgtaatct
     1561 caatttcctc gcagtcctca atgacgtcgt tgccggtaaa gccggatccg ccgatcaatg
     1621 caagcatctc actttcctca aaatgatttc tttctctggc aaccgccgac agcaggcgat
     1681 cttcaattct aaaagagccg ggaacctgat acgtgtatag ccctcgcaca ttcccgctcc
     1741 gtaacagggc cttccaccgc atcatgacga agcgcagttg aaaaattgaa caaccacgct
     1801 ccgtcaatgc ctctgcggat caggctcaga aaagggcgcc ctgctgcggg ccttcgtcgt
     1861 ccgttttccc cacctccgtc cctgcagccc gtttctcttc aggattggca ggtgtgtcgg
     1921 aagcatcctg agtactgacc gccccctcgg ccgccgcctt ctcctcttcc tcagcctcag
     1981 gcgtcacaac aagcttttcg cttgaaacgc cgccctcgag catggcaagg agagtttcct
     2041 cgtcgatcac cttcacaccc agtgtctgcg ccttctcgag cttggaaccc gcctccgcgc
     2101 ccgcaaccac gaagtcggtc tttttgctga cggaacccga caccttggcg cccgccgcga
     2161 tgagacggtc ttttgcttcg tcgcgcgtga gcgtgggcaa ggtccccgtc agcacgaagg
     2221 ttttgcccgc aacctcggag gcctccgctg caggcctggg ctccaccacg ggccagacga
     2281 cgccggaggc aacgagcgcc tcgatcacgg ttcggttgtg cggctccttc aggaaggcat
     2341 gaatgctctc ggcgatgatc tcacccacgt catcaacctt tgtgagcgcc tccacgtcgg
     2401 cggcctcaag attggcaagc gtgcagaagt gctgcgcaag cgacaacgcg gtcgcctcac
     2461 ccacatgcct gcatccgagc gcgaaaatga agcgggcgaa cgtcgtgtgc ttcgccttcc
     2521 cgatgctttc aagcaggttc gaagcggcct tcggccccat gcgcttgacg ggcttctcaa
     2581 gatccttcgt cgccgttgtc ggcgcgatga gatcctcagg cttgagcgtg aagatgtcgg
     2641 caggactctt caccagccct tcgtccacaa gcatgttgac gatcttctca ccgagcccgt
     2701 cgatgcccat ggccctgcgg gaggcaaagt gaacgaggga gagcttcatc tgcgacggac
     2761 agtacagacc gcccgtgcat cgcgtgtcct tctcctcctc gtcgcggatc gtggccgacc
     2821 cacagacagg gcagacggac ggcattgcaa acggcacggt accctctgga cggcgctcgt
     2881 gcacgacgcg caccacctcg ggaatcacgt cccccgccct gcggaccacg accgtgtcgc
     2941 cggggcgcag atcaaggccc gcaatatggt cctcgttgtg aagagtggcg ttcgagacgg
     3001 tcacgccgcc gacgaaaacg ggcttcaagc gcgcgacagg cgtgagcttg cccgtgcgcc
     3061 cgacttgaat gtcgatggct tcgcagaccg tcagagcctc ttcgggcggg tacttgtggg
     3121 cgcaggccca gcgaggctcg cgagcaatga agccgagctc cctctgaagc tcaagactgt
     3181 ccaccttgta gacgacgccg tcgatgtcga agggcagcgt gtgtctgatc tctgccacct
     3241 catcatgaaa ggtcgcgagc gctgtcggcc cctcgaccct tcggcggacg tccgcaacag
     3301 ggaacccgag cgacgcaagt ctgtcgagaa cttccgtctg cgtgggcgcg aattcgccgc
     3361 ccgagacttc acccagcgca taggcgtaaa aatgaagcgt cctgcgtgcg gtcactgcag
     3421 gatcgagctg gcgcagggac cccgccgccg catttctcgg attgacgaag gtcttttgcc
     3481 cctcctctgc ctgacggcgg ttaagcgcct cgaagtcgtc gcgatgcatg atcacctcgc
     3541 cgcgcacctc cagcacgtcg gggaagtcgc cctccagacg aagcgggatc gtgcggatcg
     3601 tgcgcgcatt cgcggtcacg tcctcccctg cactgccgtc gccgcgagtg gcggcctgta
     3661 cgagaacgcc cttttcataa cgaagattcg ttgccagacc gtcaaacttg agctcgcagt
     3721 cgtacgccac ggcaggatcc tcatccgcga gcccgagttc gctgcgcacg cggcggtcga
     3781 atgccgccgc accctcgctc gaaaagtcgg tctcggtgtg aatggaaagc atcggcacgg
     3841 cgtgcacgac ctttgcaagg tcgcttcgaa cagcaccgcc cacgcgggcc gtgggcgagg
     3901 caggactctt ccattcagga aatctggcct caagagcctt gagctcattg aacgtgcggt
     3961 cgtattcggc gtcgggaacg ctcggattgt caagcacgaa gtactcgtag tcccaggtct
     4021 ctacgagctt ctcgagctca cgcatgcggg cgggggccct ggagggctcg atcgccgcag
     4081 cctttgcggc accgtcgaag tcgtcgttcg cgagggtttc agtctttgtc ggttcggtca
     4141 tatcttgcat tccgaagaag cgaagaagaa aagcccgccg ccgggcggct tgcgtccgaa
     4201 tgcggcgggc agtgaatgat gcttgacgcg gatcctgcgt caggcgcttc tcgagaagag
     4261 gagccttgcg cgctcggagc cgggacgcac gccgctggcg gccatctgcg cgtcctgatg
     4321 ctcgatctcg tcctgaagga tcaggccgcc tgcagcctcg atgcggttgc ccgcgccgtc
     4381 gacccagacg ccgccgagat ggcaggcgat gtggtttgca agctggaaga agcgcttgag
     4441 gtcgccgcgc ggcacgttgg caagggggac gtcaaaggaa aggcgcagct gggaggagtc
     4501 ggcacccgcc agcgagagcg tcatcagagg ctctctggaa ccttcgtcga ttcgaagctc
     4561 ccaacggccg ctcgcgctga cgaagcccgc gccctcggca accttcgtca cctcctcgag
     4621 cgtcaggccc tcctcacgcg gagcggggac gatcgaaacc tcaatcagct tgtcgtagta
     4681 gtggatgaag cgggagatct gaacagccgt cgagaccgcc gtcttgatgt cgcaggatgt
     4741 gcgcacgtca acgtcgttct gggcggcagt ctgctcgagc acctgcagga agctcgaggc
     4801 ggaaacgtcg tcaagcacgg cggcacgatt ggcgatcagg atcgtcgcga cgatgtgaac
     4861 ggcctccgca ggcaggcatt cggacggata gtagaggccg tcctcagcgg acttcgccca
     4921 aagcgacacg ggcagcggca gcgaaagctg tcggatctgc gtgacaaggg agtccatgga
     4981 accgagcggg aaggtcttgc cttcataggg cgtcacgtcg agcacccact ggatgccgtg
     5041 gtcgacgggc ggcttttcga tgccggcgat gcggcctgcc tttcgcccgc cgcgggcggc
     5101 ctcgcgctca aggcgcgcct tctgctcctc ggcgaaacgc tcgtagacgg gcggcttttc
     5161 agacggaagg tcgtccctgg aggcagactg aaccgcctcg tgcggctcgt cgtcgtccac
     5221 catgtcctcg agctgaggct cgacgcgagg ctcgggcttc tcttcggcga gcacgtcctt
     5281 gcgaacgtcg cctcccgcct gcaggcgggc ctttcggctt gcgcgcatgc ggcgctcctc
     5341 gcgcatcgag cgccagaaga agaaggcaat gaccaccagg gcggccacga taagaatgat
     5401 ctgagtctga ctcatttgaa ttatgtccgg ctttgtgggc gcccgacgcg ggcgcagctt
     5461 ctgttgattg tacgcacttt tgacggattc gccacgacgc gcgtgcggga cgcggaaccg
     5521 caggcgtcag ttcgccatgc ggaccgcgtt ctcgatgtcg acgctcacga cgcggctcac
     5581 gccgggctcc ttcatcgtta cgcccacaag cgctcccgcg aactccatcg tcacgcgatg
     5641 atgcgtgatc atgaggaact gcgtctcgct cgacatgcgg cggcacagac ctgcaagcct
     5701 cgcctgattg gcctcgtcaa ggggggcatc cacttcgtca agcaggcaga agggagcggg
     5761 attgagcctg aagatcgcaa agacgagcgc cgtggccgcg agggcctgct cgccgcccga
     5821 aagcagcttc acgcccgcgt tcttcttgcc cggcggctga gccttcacct cgacgcccgc
     5881 gttgagcaca tcgtctcccg acatgacgag cgaggcgacg ccgcctccga agagctcgga
     5941 gaacgtctcg gcaaagtgac cgttcacctc ctcgaacgtc tccctgagac gtcctctggt
     6001 ctccgcatcg atctttctga tggcggcctc cagagtttca atgccctttt caaggtcctc
     6061 cacctgtctg gccgtcgcct cgagcgtcct tctcaccgcc tcgaggtgct cgagcgcggc
     6121 atgattgacg ggacccaatc ctgcaatctc gctgatgagc ctcagcacct tggcgcgaag
     6181 gctctgagcc ttctgcggac gctccgccgc gcgacgcgca agatccgtcc tgtcggcctt
     6241 gagctcattg agcctctcct caaactgagt ccgaaggctt tccttcatct gctgctcgac
     6301 tctcttgacg ccgagcgcct ccgtcatggg catgacgtcc gcctgcgcgg cctgccagcg
     6361 gccctgagcc tcggtgagcg caagtctggc cgcggccgtc gcgtcgagac gtgcggcatg
     6421 cgcacgctcc gcctcttcaa gcaccttgag ggcagcggcc gacgccgcgt cgccgagcac
     6481 cttcgcgctc tcctcaagga gcctggaggc ctcgacaaga cgcgcctcct ctcgggcgat
     6541 gtcgcttcga agaaccgcct cgctctgtgc atacagacgg gccgactccg aaagctgccg
     6601 cagcttcgcg cgtgccagct ccgccttgtg cctcacggcc gcaagcgcag ccgtcttcgt
     6661 cttgaagcgc atgtcggctc gcgcaagttc ggacgtgaat cctgcggcag cgcgcacggc
     6721 gcgttctgac gccgcctccg cctcgtctgc cgctacgccc gcctcttcgg caagcgctct
     6781 ggcctcctcg ccttcggcct caagctcctc cctgctgcga cgcagatcgg caagacgggt
     6841 ctcgtgggcc gccaccgccg cctccgccgc cttgatcctg agcctcaggt cgaccacccc
     6901 cgcctcagca gcacgggcgc cctgagccga agtgcgtgcg gtccgttcag cggcctcggc
     6961 cgccgctccg gccgcattcc tgtcgtcatc aagcttgccc agcctgtcct caagctcatg
     7021 aaggcagtcc ctgagtgcgg agatctcctg ctggcgagag agcacggact gtcccggatc
     7081 gctcgccgtc cagacgacaa ggcttcggct cgtgagccgg tcgcccttcg gagtcaccag
     7141 cgtgacgcct tcgggaagcc tggctctcaa agccagcccc tccgaaaggc tcttcacgca
     7201 gtagacgttc gccgcaacgt ccgcgagagc cgccgtcgcg tcggacgaac gacacctgac
     7261 gtgcgaggtg atgggttcca gcctgacgcc gtcgagctcg agtgcagggc ggccctcctg
     7321 cgtctcacct cgttcaacga acgagatggc ggcgggcggc cggcggctct cgtagccctg
     7381 agccatgccg agccggctca agaagcgtgc tcccagcctc gatccgacaa cggcctcgac
     7441 ggccgtcacc cattcggggt cggccgaaag cgcctcggac aggcgcggaa gaccgtcaag
     7501 cccttcggcg gcctcgaatt cggcaagccg cccctttgcc tgagcccccg cctcgacggc
     7561 ctcaagggcg tcgagcttcg cggccgtttc cttttcctga gcaagagccg caaaataagc
     7621 ctcgttggcg gcgtctcttc tgcgtctcgc ctctgctgcc gcctcagccg cctcctcttc
     7681 aagtgcgcgc gcctcctctg cggcggcctg agcctcctcg aagtcgagct tcatcgcctc
     7741 gagcccctcc ttgtcaggcg cttcgagcgt tccctcggaa gctgcaagcc tcagaagacg
     7801 ctgtctgaga tcctcggcac ggcgctcggc cgcctcaagc cgcgcatgtg caacacgtgc
     7861 ctgagccttc gcgtcggact cggctcttcg agcttcggcc gcgcgggcat tgacgtcgtt
     7921 cagagcgtcc tcggcggaga gaacctcctc ctgtgccgca gcctcctctt cgcccgagag
     7981 cgcaatcgtc tcctcaagct cgtcagtctc ggatgcctgg gcctctctgg cggaaacagt
     8041 ctcggcaagg cgcctttcct tggccgcctt tgcctctcgg gcttcggctg cggctctttc
     8101 cgctccggcc ctgcgttcgc gctgccgtct ttcctcggct tcggctttgg tgagcgcacg
     8161 ttcggcctgc ctcagtcctg cagcggcctc aagctccttc gcctcagcct cgcgtgccgc
     8221 ggactcgagc gcgggaagcg acgccgcttc acgggcggcg tcggcctttc tagcctcgat
     8281 ctcggcctcg ccgcgcgcaa tgtcggcggc aatcccgtcg agcgttcttt tcacgtcctc
     8341 atactggatc cagtaccaga gggattcggc ggcgttgcgc tcgtccgtga gcgcacgcca
     8401 ctttcctgcg agctgcgcct cgccctcaag ccttgcggcc tcctcttcct tgacggcctg
     8461 catgtcgcgc acgcgctcga ggttgcccct cgtctgactg agaagcgact ccgtctccct
     8521 gcgtcgctcg cggtagagcg agacgcccgc ggcctcctct agatagacgc gcatctcctc
     8581 gggcttggcg cggatgaagt tcgagatcat cccctgggag atgatggcgt agctgcgagg
     8641 cccgagcccc gtgccgagga agatctcctg cacgtctcgg cggcgcacct gctgatgatt
     8701 gatgtagtag gcggactgac cttcggccgt cacgactcgc ttgacgctga tttcgccgaa
     8761 ttccgaccac gcccccttta ggcgtccgtc gtcgttcgtg aggatgagct ccacgctcgc
     8821 gcgcccgaga gcgtggcgcg tggtcgagcc cgcgaagatc agctcacgca tcgaggacga
     8881 gccgcgcagt tcagagatgc gcgcctcgcc gagcacccag cgaacggcgt cgatgatgtt
     8941 cgacttgccg cagccgttgg gtccgacgat cccgacaacg ggatgctcga gctcgatgag
     9001 cgccggatcg gcaaagctct tgaagcctgc gagcttgatc tgacgtaagc gcaagaaggt
     9061 ctcctgaaaa ggtgcggccg gttgatggaa ggcccgttgc gccgcagacc ggcggcgcaa
     9121 acaggcgtgt ctgcctattc taaatgaggg ccgcccgcgg gacatccccc tgcacggacg
     9181 gtcgcccccg ccgaccttat aatgtcgggc atcgccgcag acgcgccgtg tccgatcctt
     9241 cggacctgcg catcgcctgc gcgccctccg ttttccgcgt tcccgcgggc gccgcattcg
     9301 ccgcgtccgc ccctttactg gataccgcca tgtacgaaat tgctccgctg cttgatgacc
     9361 ccattcacaa tctgaagacc atccgcgacg tcttccgctg ggcgatcacg gaaatggaaa
     9421 acgccgagat ctcctacggc cacggctctc ccgacagttg ggaggaagcc tccttcctca
     9481 tctgccgtgg cctcaagctt ccgttcgacc acttcgacat gttcctcgac gccgcgctca
     9541 cgcacaacga gctctcccga ctcgtgcacc tcatcgaccg ccgcgtccac gaaaaggttc
     9601 cgaccgccta cctgctgcgc gaggcctggc tcaccgagca ccgcttctac atcgacgagc
     9661 gtgcgctgat tccgcgctcc tacattgccg agcttctcga ggaggacctt gccccctggg
     9721 tcgaggatcc gtatgaagtg ggcagcgtgc tcgacctctg cacgggttcg ggctgcctcg
     9781 cgattctcgc ccagggcacc ttcccgaacg ctgaagtgac gggctccgac ctctcgcccg
     9841 acgcgctcga ggtcgcccaa atcaaccgcc gcgactacgg catggaggag acgctcgagc
     9901 tcgttcaggg cgacctcttt gaaaacctgc agggccgcac gttcgacatc atcatctcca
     9961 acccgcccta cgtcacgcag gcgagcatgg acacgctgcc ctccgaatac cgtcacgagc
    10021 ccgccatggc gctcggcgca ggcgacgacg gcatggacgt cgtgcgccgc atgcttcccg
    10081 aagctgccgc acatctcaat gacggcggca tgatcatcgt cgaagtgggc gacggcgccg
    10141 aagcggtcga ggcgaccttc cccgggctcg aaatcatctg gctcgccacc tcgggcggcg
    10201 acgaccaggt cttcctcgtc acaaaggaag cgctcctcaa ctacttcgaa cagtaatcca
    10261 gcgtcatgct ccgacttctc aaccttgcgc tctcccgcgg cacccgcgtc ctctaccgtc
    10321 atgcgacgct cactgcggcg ccgggcgagc gcatcggcct cgtcggcccc aacggctgcg
    10381 gcaagtccac gctctttgcg gccgtgctcg gcgacctctc tcccgaagac ggcgacatcg
    10441 agacgccgcc ccgcgacagg atcgcccacg tggcgcagtc cttcgtcgcc gacaacgtcc
    10501 ccgccgtcga cttcgttctc ggggggcacg ctcccctgat cgccgcccgc gcggcgctcg
    10561 ccgaagccga aaaggcaggc gacgacatgc gtcttgctca ggcccacgcc aatcttgccg
    10621 agctcaacga aggcgcgatc gtcgcacagg cccgtaccat ccttgcgggt ctcggcttca
    10681 gccagaccga cgtcgaccgc cccgtctacg acttctcggg cggctggcga aaccgcatcg
    10741 cgctcgcacg cgccctgatg tgccccgccg acctgcttct ccttgacgag ccgacgaacc
    10801 atctcgacat cgactcgctc atctggctcg agaactggct gcgccgcgtc gaggcgaccg
    10861 tcgtcatcat ctcgcatgac cgcgagtttc tggaccgcgc cgtcacgacg atctggtccg
    10921 tcgaggacga caccgtctgc cgctacgcag gcaactactc cgccttcgag tcccagcgca
    10981 tcgaaaagct caggctgcag gatgcggccg ccaaggccta cgagcgcgag gcgtcccatc
    11041 tctcgagctt catcgagcgc ttccgcgcca aggcgacgaa ggcccgtcag gcccagtccc
    11101 gcatcaagat gctcgaaaag ctcaaagccg tcgagcctgt gcgcgccaag cgcgagtggc
    11161 ggttcgaatt tccgaagccc gtgcgcctgc ccgagcatct cgtcgacacg gagaacctgc
    11221 gcctcggcta tgacgagcgc gtcgtgatcg accgcgtcac gctctcgatt cgctcgggcg
    11281 agcgcatcgg catcctcggt gtcaacggcg caggcaagtc cacgctcgtc aagggcatcg
    11341 tcggagacct tgctccgatg gcgggcgaac ttcgtcgcgg ccagggtctc gagatcggct
    11401 atttcgctca gcatcagctc gatcagttgc gtcccgacga aagcccgctc gagcacatgc
    11461 gccgcatggc gcccgacgtg cgcgagcagg accttcgcga ctacctcggc atctaccgct
    11521 tctcgggcga ctttgcgtct gcccccgtcg gcccgatgtc gggcggcgag aaggcccgcc
    11581 ttgcgctcgc actcctcgcc tggcgcaagc ccaaccttct ggtgctcgac gaaccgacga
    11641 accacctcga catggagacg cgcgaggccc tgaccatggc gctctccacc tatgagggcg
    11701 cagtgctgat cgtctcgcat gaccgccatc ttctgcgtgc cacgaccgag cgcctcatcc
    11761 tcgtgcacga cggggccgtc cagccctatg acggcgacct tgacgactac gcccagctcg
    11821 tgcttgaaca ccgccgcacc acggtcgcag ccgaaaaggc ggcgaatcag gccgaaaggc
    11881 ttgagtccgg cgagcgcgag ccctcccaga accgcaagga ggcccgccgt gccgaggctc
    11941 aggagcgcca gcgcatcgcc gccctgcgca agcccctgca gaaggagctt gccaaggtcg
    12001 aaaaggaact cgcgcagaag aacgaggagc tcaggaagct ctccgatcgc cttgccgacg
    12061 gcgacttcta cacctcgact gatcccgacg aagtggcccg cgtgcttcgc gaacacggcg
    12121 agctcaagcc cgtcgtcgag gagctcgaaa tgcgctggct cgaactctcc gaggaaatcg
    12181 aggccgtctg ctgacgtcct ccgagccgca ccttttctgc aaggccgtcc cgcaaatggg
    12241 cggcctttct tcattcggcc ccgaccgacc gatgcgaaag gcatgcgcat cggctccctc
    12301 acgcgtcccg cgccctcttt tgctgatatg attcacgcaa atcggaaagc cccggcggcc
    12361 ctcaggggcc gtcacgacca ggggtccttc ctcccgacaa cgccctttcc gcccgtacgg
    12421 agtcccacat gcgcagatac aacattgcag cgacgcccgg cacgctgcac gccttcaagt
    12481 ccaaacgcgt cgtcgtcgac ctcaacaagg ccgacctcac gagcgtctcg gccgtcgtga
    12541 tgacggccga cgaggcgctc tcgggccagc tcgaacgcgt cgaggcgacg gcgttcggca
    12601 ttcccgtcat tctgctcaac aacggcgcca agctcccgac cgagctcttc ggcaaggtcg
    12661 tctccgtcat caactcccag cccgacaacg ttggcctcta cggtcgcctg atcgacgccg
    12721 ccgcccagaa gtacgaggat cagattcttc cgcccttctt cggcaagatg gagtcctacg
    12781 tccgcaacgg caacgtgcac ttcgactgtc cgggtcacca gggcggcgcc ttcttccgcc
    12841 gtcatccggc aggccgtcag ttcgccgact tcttcggcga aaacgtcttc cgctccgacc
    12901 tctgcaacgc cgacgtcgcg atgggcgacc tgctcatcca cgaaggcgtg ccccgtgccg
    12961 cccagatggc ggccgccaag atctacaacg ccgacaagac gtacttcgtc ctcaacggca
    13021 cctccgcctc caacaaggtc gtgctcaatg cgcttctcac gccgggcgat ctggtgctct
    13081 tcgaccgcaa caaccacaag tcgaaccatc acggcgcgct cattcaggcg ggcgcgactc
    13141 ccgtctacct cgagaccgcc cgcaacagct acggcttcat cggcggcatc gacgaccact
    13201 gcttcgacga gaagtacctg cgcgagctca tccgcgaggt tgcgcccgag cgcgccgatg
    13261 aaaagcgccc gtttcgcctc gccgtcatcc agcttggcac ctacgacggc acgatctaca
    13321 acgcccgtca ggtggtcgac cgcatcggcc gcctgtgcga ctacatcctc ttcgactccg
    13381 cctgggtcgg ctacgaacag ttcatcccga tgatgaagga ctgctcgccg ctcctgctcg
    13441 acctcggtcc cgaggatccc ggcatcttcg tgacgcagtc cgtccacaag caacaggccg
    13501 gcttctcgca gacctcccag atccacaaga aggaccttca catcaagggc cagaagcgtt
    13561 actgcaccca caagcgcctc aacaacgcct tcatgatgca cgcgtccacc tcgcccttct
    13621 atccgctctt cgccgcgctc gacgtcaacg cgaagatgca cgagggtgaa tccggccgcc
    13681 gcctctgggc cgactgcgtg cgcgtcgcga tcgatgcgcg caaggaaatg ctcgcctgct
    13741 gccactacat caagcccttc attccgccca cggtcgacgg caaggcctgg gacagctatc
    13801 cgacggagca gatcgcgacc gacatccggt tcttcgcctt caatcccaag gacaagtggc
    13861 accacttcga aggctatgca ccgcagcagt acttcgtcga cccgtgcaaa ttcctgctca
    13921 ccacgccggg catcaacgcc acgacgggtg agtactccga cttcggcatt cccgcctcca
    13981 ttctcgcgag cttccttcgc gagaacggcg tcgtccccga aaagagcgac ctcaactcga
    14041 tcctcttcct gatgacgccc gccgaggacc atgccaagat ggcgcacctc atcacgcaga
    14101 tcgcccgctt cgagagcttc gtcgacgatg acgcgccgct ctccgaagtg ctccccgagc
    14161 tctataacgc ccacaaggag cgctacaagg gctacacgat ccgcgagctc tgccaggaga
    14221 tgcatgactt ctacaagtcc gtcaacgtca agggcctcca gaaggcgatg ttccgcaagg
    14281 agtacttccc caggcgtgtg ctcaacgctc aggaggccaa ctacgagttc atccgcgaca
    14341 acgtcgagct cgtgcgcctg agcgaggccg aggggcgagt gggcgtcgaa ggcgcgcttc
    14401 cctacccgcc gggcgtcctc tgcctggtac ctggcgaagt ctggggcggc gccgtgctgc
    14461 agtacttcct cgcccttgag cagggcatca acctcttccc gggctttgca cccgagctgc
    14521 agggcgtcta catcgaggag gacgaagacg gccgtcaggt cgcctgggcc aacgtgctca
    14581 cgcacgagcg cgagaccgaa ctcctcggca aagccctctg acctcgattc cgtcgggaaa
    14641 cggcccggaa gccgcaaggc ctccgggccg tttttgtctc cggtcccgca ggctaagcac
    14701 attctcctag agacgattac ctatcaggcg ttttcagtga agaaaacttg aggtattacc
    14761 tttcacggat gcacggcatc ccgcaaaatg aatccatcgg acggccgcat acgcattcga
    14821 tttcccatac caacaggccg cccccaagga gttcatcatg caaaagcgca ccttcatcaa
    14881 gtctctcgcc gccgtcacgg cgctcagcgc catagttctt ctcacgggct gcgacgagga
    14941 aaaggcggtg gctgaaaagc ccttccgcgt gggcgtgacc gctggtccgc acgccgccat
    15001 cgtcaccgaa gccgcaaagg tcgcggcgaa gaacggcctc aaggtcgagg tgatcgagtt
    15061 caccgactac gtgacgcccg acacggccct caacgacggc gcgcttgacg ccgcggtcta
    15121 ccagcacgag cccttccttc tcaacttcaa caagcagaag ggcacgaagc tcgccaacgt
    15181 gggcaacgcc gtggtgcagc cgatgggctt ctactccaac aaggtgaagt ccattgccga
    15241 cattcccgaa ggggcggtca tctccgtgcc gaacgatccg acgaactgcg gacgcggtct
    15301 tctcctcctg caggccgcgg gcctcatcaa gcttcccgaa ggcatgggca gcgaggcctc
    15361 tctcgccgac atcgtcgaga acaagcgcaa cctgaagatc agggagctcg aggccgccca
    15421 gctgccccgt agcctcgatg atgcgctcgt cgccgtcatt ccgatgaact acgtcatcag
    15481 cgcgggcctc tccccgcaga agcagggctt ctacttcgag tccctcgagg cgccgttcgc
    15541 gctcatcatc atcgccgcgc atcaggaccg caggaacgac aagagcgttc aggacttcgt
    15601 caagtactac cgctcgcccg aagtcaaggc cttcgtcgaa aagaccttca acggcacgat
    15661 caagccgagc tggtaatcac cgccttctgc gcatgacctc cctcagggcc cgcacggact
    15721 gccttccgtc gggccctagg cgttttccat gacctcctct gttgaacggg catgtgaact
    15781 gctaccataa cggcgcgcgc ccgccactcg cagtagcgga tggacgggac gccgtcagga
    15841 tggcgccatg aatttcacct cacttctcca aggacggcag gcctgacgcg gccggcctgc
    15901 gtcctcccgg ggctttcgcc ctccggactc ccgtcggcac gatcattcca acaacacatc
    15961 caaccgacaa gggagaccga aaaaaatgct cggcatcctc attaccgtcg cagtcatcgc
    16021 cgtcgtggcg tactgcgtgc tcaagaacta ctacccgccc atcgtccttc ttctcgcagg
    16081 cctgctcatg ctcgtgtgcg cctggctcat gggcgtcgat cccgtcgacg cgaagaagag
    16141 cacgaacttc gtgggcttcg acttcgttca ggccttcacg aaccagatga agggccgcct
    16201 tcccggcctc ggcctcaaca tcatgctcat cgcgggcttc tccttctaca tggaccgcat
    16261 cggcgcctcc aaggcgctcg tgaagctctg cgtcaagccg ctttccgccg tccgctcccc
    16321 gtacgtgctg ctcgccatca cctacgtggt gggccagttc atggcgctct tcatcaactc
    16381 cgccgtgggc ctcggtcttc ttctgatggc cagcgtctat ccgctcctcg tcgcgctcgg
    16441 cgtctcccgc gcctcggccg ccgccgtgat cggctcgacc tgctgccttg acctcggtcc
    16501 ctcgtcctcg aacgccatgc gtgccgccga cctgatgtcg accgacgtcg tcacctactt
    16561 cgtccagagc cagattcccg ttgccgtctg cgtgatcgcc acggtcgcca tcggccactt
    16621 ctttgtccag cgctggttcg accagcgcga cgctgcccgc ggcgtcgccg acggcgctcc
    16681 cgtcaaggcc gaccagaagg acctcgagaa ggccttcgaa ggcgcgggcc ccgtgcacta
    16741 cgcgcttctg ccgatgctcc cgctcgttct cctcatcgtc ttctcgccga tggtctacga
    16801 cggcatcaag ctttccctgc agaccggcat tctggtctcc atcctgatcg ccttcttcgt
    16861 cgacctgatc acgcacttcg acttcaagga atgctgcaag cgcacgagcg ccgtctttga
    16921 aggcatgggc aaggtcttca cctccaccgt ggccctcatc tgctgcgccg aactcttcgc
    16981 gctcggcatg aacaagatgg gcggcatcac cacgatgatt caggccgcag ctgccatgga
    17041 aagcgccggc gtctgggtga tgctcttcat catgctcggc atcatggtcg tcgccacgat
    17101 cgtgacgggc tctggcaacg ccgccttctt cgccttctcg ccgttgcttc ccgaagccgc
    17161 cgccagcgtg ggcatcaaca ccgccgtgct cgtcgtgccc gtccaactct ccgccggtat
    17221 cgcccgcacg atgtgcccga tcgcaggcgt gatcatcgcc gtcgccggca ttgcgggcct
    17281 cacgcccttc gaaatcatca ggcgcacgat tccggtcatg cttctcgcgc tgatcgtcaa
    17341 cgtcgccgcc tccgccgtct tcctctaacc acggaatacg gccttcaccc aaggagaaac
    17401 tgaaatggct attctcatca agaacgcacg cgtcttcgcg cccaaggacc tcggcgtagt
    17461 cgacgtgctc atggccaatg aacgcatcct cgccgtcggc aaggacctcg cccccaacct
    17521 ccccgacctg cagacggtcg aagccggcgg catgatcatg accccgggct tcttcgacca
    17581 gcacatccac gtcacgggcg gcggcggtga aggcggtccc gccacgcgca cgcccgagct
    17641 cgtgctctcc gaactcgtcg cctgcggcac gaccgacgtc gtcggcgtct ccggcaccga
    17701 ctacacgacg cgctccatcc caaaccttct cgccaaggtc cgcgcccttc aggccgaagg
    17761 cgtctccgcc tggatgtaca cgtccaacta ccgctgcccg ccgacgcttc tcaccgattc
    17821 catcggcaac gacctcttct tcattcccga ggtcctcggc gtgaagatcg ccctcggcga
    17881 ccaccgctca agcttccccg acgtccagac ggtcctctcc atgctcgccg acatccgcgt
    17941 cggcgccatg ctcgcaggca agatcggctt cctccacatc cacaacggca acattcccgg
    18001 cgccttcgcg atgtatgagg aaatcgtctc gcgcggcttc ccggtcaagc acatccgccc
    18061 gacccactgc ggccgcatcc gccacgtctt cgactccgcc gtccagttcg ccctcaaggg
    18121 cggctggatc gacatcacga cgggcgcctc ctgctgcttc gaccatcctg cccaagccgt
    18181 cgtcgaggcg atcgccgcgg gcgtcgaccc gacgcacatc acgctctcga ccgacggtca
    18241 cggttccgtg ccccgcttca acgacaaggg cgaaatggtc ggcctcggcg tgggcggcgt
    18301 ggacggaaac ctcaaggaaa ccatccgtct catccgcgat cacaacatgc cgatcgaaca
    18361 ggccctcacg ttcacgacct cgaacgtcgc cagcgccctc ggcatcaagg gccacggcgt
    18421 catcgagacg ggcgcctgcg ccaacgcctg cctcttcacc gaggacttcg agctcaaggg
    18481 cgtcttcgcc cgcggccgcg aaatgatgcg cgacggtgaa atcctcgtga agggcacgtt
    18541 cgagtactaa cgccgaaaca ggccgaaagg cccgcgtccg cacgttttca ggagccggtc
    18601 ctccctcagg gaggacggct cctttttttc gacaactctt caacaatcag gggctttgcc
    18661 tgacccattg aggagattta caattccctt tggtgcgtcc gcccgtgcgg ccgcatgttg
    18721 aaaagaaaaa agaggaccac atggacattg aactcgaatg gctgcaggcc accttgaacg
    18781 atgcctccga agcgcttcag gacgcgctcc agaaggttga aaacggcgat caggaagacg
    18841 cgctcgaagt gctcaaccac gacctcgtgc acgtgtacgc gaagctcaac tacgccgtca
    18901 acacggcccg cctcggcccc gcggctctcg acgccatgag cgaggacgaa ctgatcggct
    18961 ggcccgcccg catgcccttc ctcaccttcg acgagatcga caccctcggc gaggacgaag
    19021 aggatgaaga agcggtcgag gaagaggacg tcaaataagg tcctgactcc cgaacggaca
    19081 gacttcaaaa aggaaaaggc gcggatctgc tgtccgtgcc ttttcctttt tcaatgttcc
    19141 tgatttcgac cctcagtcgt ctccgcgtcc gggacgcagg tcggagaggc gccagccgca
    19201 agcgaacatg acggcgaagt aggcgacggc tgcgaccgcc accatggcga gcacaccgcc
    19261 cgcgcgcatg agccacggca tcgtggtcca ggagacgaag gtctgcccgt accagagacc
    19321 cgcacccatg aggagagtgg cgagcgcgat gcgaagcacc gccttcgccc acccgggaag
    19381 cggcgtgtag atgccgcggc gcatcaggat gatcagaagc gtacccgcat tgaagacgct
    19441 gccgacgccg accgaaaggg caagccccgc atgcgagaag agcggcacgg agaccaggtt
    19501 gacgagctgc acgaccacaa ggctccagaa ggcggtcttg acgggcgtac ggatgtcctt
    19561 gcgggcgtag aaggcgggag caacgatctt gagcgcaatg agcccgatga ggccgaccga
    19621 ataccccacg acagcgagtg cggtctggct cacgtcgtcg ggcgtgaagc ttctgccttg
    19681 gaagaggaag gagacgagtg cctcgctcgt gagccagagg ccgacggccg cgggcacggc
    19741 gacgaggacg acgagcctca gtcccctgtc gaggagcgag ttgtagcgcg acgtgtcgcc
    19801 cttcgcatag gcgctcgaga gaccagggag gagcacggtg ccgagcgcaa cgcccagaag
    19861 cgccgtcggg aactccatca ggcggtcggc gtagttgagc caggtgaccg cgcccttgcc
    19921 gagatgggag gcgatgttcg tgttgatgag aatcgaaagc tgtgcgacgc ccacgccgaa
    19981 aagcgcgggc accatgagct tgaggacgcg cctgacgtca ccgtcggcca gactgtcccg
    20041 aagcccgcgg ggacgcaccc ggatcccgag cctgcaaagc gccagatact gggtgccgag
    20101 ctgcagtacg ccgcccagga tcaccgccac ggcaagcgcc cagatgggct cggccatgcg
    20161 aggcgcgaga agcaccgtgc agccgatgaa ggagagattg aggagaacgg gcgtcaccgc
    20221 cggcaccgca aagcgcttcc aggtgttgag gacggaagcg gcaagcgccg tgagcgccat
    20281 gaaggcgatg taggggaaca tgaagcgggt gagcgcgctc gcgaggtcga aggcctcggg
    20341 ggccgacgca agaccgctcg cgatcgccca aacgagcagc ggcgccgcca cgacgccgag
    20401 gatgctcgcg gcgaagaccg aaagcgcgag cacggtgaag acgtggtcga tgaaggtgcc
    20461 cgcctcgccc tcgcttctct ccttcacgtc ggcgagcatc ggaacgaagg cctgctggaa
    20521 ggctccctcc gcaaagaggc ggcgcagcat gttcggaagc ctgaaggcaa cgtagaacgc
    20581 gtcggtctcg gccgaggcgc cgaagaagcg cgcgatgagc atgtcgcgga tcacgcccgt
    20641 gacgcggctc accagagtca ggcctgaaac agtaagagca gacttcaaga gcgacatgtg
    20701 tcatcccgtc ggaaattgga aaatcgcaaa aaattctact agaaaacgcg ccctcggctt
    20761 ggaaatttga tatagttgag ggcttttcgg agtcacgcaa ttcaaagcct ccgttctgac
    20821 aggccgactg atgccccaac ctcagacgga cgaaggacgg aagtgttgcg caaggctctg
    20881 gaaattcacg aaatctttgg tgtctcgcgc aaccgcgatg cactttcccc gcctcaagcg
    20941 gaggttatgc agcggcgtcc ggacagcgca tgatgcgcac cacgggaaca ccgggatcag
    21001 gcatcccatc gggatgctct ggacgccaac tgtttaattt acaggcaagg aatttaaaaa
    21061 aatggccaat accaagcaag ctcgcaagcg cgcccgccag atggttgtgc gcaacatgca
    21121 cctctcggct cagcgcagcc agttccgcac cgccatcaag gccgtgcgca agctcatcgt
    21181 cgccggcgac aaggccgccg ctcaggaagc cttcaagaag gctgaatccg tgatcgactc
    21241 catggctcgc aagggcgttc tccacacgaa cgccgctgct cgccacaaga gccgtctctc
    21301 cgcttccatc aaggccatgg cctaatcgga acgtcggatc ctgacaaaaa acgcacctcc
    21361 tcgcggggtg cgtttttttt tggttcatct cggaagtccg tggaacgcgg ccttgacttt
    21421 taagaaaaag tctggtcggc tccctccaag aaaaaaaagt agcgctcact ccaaatttca
    21481 gg
//
LOCUS       DBMDTI010000010        20410 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_876_length_20410_cov_3.090206, whole genome shotgun sequence.
ACCESSION   DBMDTI010000010 DBMDTI010000000
VERSION     DBMDTI010000010.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 20410)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20410)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20410
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_876_length_20410_cov_3.090206"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            64..669
                     /gene="scpB"
                     /locus_tag="ACZPB6_01395"
     CDS             64..669
                     /gene="scpB"
                     /locus_tag="ACZPB6_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR1183164.1"
                     /translation="MTPQHIIEAALLSSRRPMPVREIRRLFDDRLSAKSVKEHLAELQ
                     AFWEDRGMRLVELSDGWRFQTASELGPRFLRLDEEKPPRYSRAAMETLAIIAYHQPVT
                     RGDIEELRGVTVNPATLRLFEERGWIETVGYRESPGRPALLATTRTFLNDLGLRSLAE
                     LPGPDAEPLPDFLLSEALPPSAAADEGPMPLQKEIDFEKQQ"
     gene            653..1900
                     /gene="rluB"
                     /locus_tag="ACZPB6_01400"
     CDS             653..1900
                     /gene="rluB"
                     /locus_tag="ACZPB6_01400"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HIR1183165.1"
                     /translation="MRNNSNSRGVRRPQSAERKRTPFRRPGEGNLPRTRRVREDAPFS
                     ERAGLASPREAFGQMESEADGYRGFADRPIALDIIDRARQQQTPADLPTEKLQKALAD
                     AGLGSRRDMEALIASGVVTVNGQTATVGDRVTAADMIRVEGRLVRRTMAASSTPRVLM
                     YHKMAGEIVSRDDPEGRPSVFARLPRVSGARWVAVGRLDFNTEGLLLFTTSGALANRL
                     MHPRYEIDREYAVRVIGELEAEQMLQLQQGIELEDGMAKFDKLEDQGGTGMNHWYLVQ
                     IREGRNREVRRMFEAVGMTVSRLIRVRYGAVSLPKTLPRGKRMELTPEEVRAWMLDLD
                     EAEKKMAAAAPAKAEAKKAEKAAAREKARARARAESEAEAREARRGTRFKEGKSDRTG
                     VPMWEARDMSDRPTQRRRTGERS"
     gene            2319..2885
                     /gene="rimP"
                     /locus_tag="ACZPB6_01405"
     CDS             2319..2885
                     /gene="rimP"
                     /locus_tag="ACZPB6_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR1183166.1"
                     /translation="MTQSGALNELVERTVEGMGYEFVELERLARGLVRVTIDTTAEGG
                     ISLEDCERVSDQLTHLFTVEDIPYERLEVSSPGVERPLKRARDWDRFAGELAHVELFA
                     PLHAEGFPEAGRRKLDGRIISIEGESGAETITFDFFEVFIARTPTAAVTALRGRRKKT
                     AEVPPVRVTFPLSDVDNAHLLAELNFKG"
     gene            2891..4384
                     /gene="nusA"
                     /locus_tag="ACZPB6_01410"
     CDS             2891..4384
                     /gene="nusA"
                     /locus_tag="ACZPB6_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR1183167.1"
                     /translation="MNCREMLQLVDVLASEKNVAKDTVFGVLEAALASAVKKAQFPGE
                     DADVVVHVDRVTGEFKAARRWLVVADEEGLQEPDRQEMYSDVIDEYPEIQPGEFIERE
                     IEVIDVDTTGRRFAQDAKQVILQRLRDAEREQILKEFLDRNESIVTGTIKRIDKGDAI
                     VEIGRLEARLPRNQMIPRENLRTGDRVRAYVDHVGESNKGQQVFLSRTSPEFIKKLFE
                     LEVPEIEEGLLEIKSAARDPGVRAKIAVYARDRRIDPIGTCVGMRGSRVNAVTNELGG
                     ERVDIVPWNEDPAQFVVGALEPAKVRSIVMLEETHTMEVVVDEDNLAIAIGRAGQNVR
                     LASELTGWQINIMTEAAAAQKRDEEVAKIRADFMDSLDIDEAAADVLIGEGFSSVEEI
                     AYVPEKELYAIEAFDRETVDELRQRARNKLLADAITREENLRKADEPLLSLEGMDNDL
                     ASQLVGHGVKTLDDLGELATDDLVEMTGIDEERASSLIMKAREHWFE"
     gene            4634..7483
                     /gene="infB"
                     /locus_tag="ACZPB6_01415"
     CDS             4634..7483
                     /gene="infB"
                     /locus_tag="ACZPB6_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR1183168.1"
                     /translation="MASKTVSEFAQELKVAAKTLLAQLRAAGVNKASETDELSETDKT
                     RLLDSLRVERTQSAQRKITVTRKEQSVVKQQDGSGRSRAIPVEVRRKRVFVKNPQVLA
                     EEAARAEAEAAQARRAAEEKAAAEACARAEAEERARAEEARRAEEARIREEEERRAAQ
                     ERAEAARREAEEARAREEALKKEQEKDEAALKAARREAEKAKLDAEVAAATAKRMEAE
                     AAERRRREEARRKAEEEARSIRAMMNRKNGVLTAKKVKPAEKPAEAPKAAEQKPAAKD
                     DKAKKTARPQRPAAEPQQNAGDGRKGHPSDRKKGGKGGSDRFGDEGQHRRTIKTRGED
                     DSGQTGWRTARGRRSQDRNRHAEPVQQVQEQVVREVQVPETISVADLAYKMAVKATEV
                     IKTLMKMGQMVTINQMLDQDTAMLIVEEMGHKAVAAKPDDPEAILGELQNVEYPALPR
                     PPVVTIMGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVKTPRGIVTFLDTPGH
                     EAFTAMRARGAQATDVVILVVAADDGVMPQTKEAIAHSRAAGVPLVVAINKIDKPEAN
                     PERVKQELVANDVMPEEWGGDVPFCPVSAKTGAGVDELLENVLLQAEMLELKAPVEGP
                     AHGIVIESRLDKGRGPVASILVQGGTLHRGDIVLVGQEFGRVRAMINELGKAQQAAGP
                     SIPVEIQGLSGVPAAGDEVIVLNDERKAREISLFRQGKYRDQKLAKQHATNLANLFSG
                     ANEGEVKTLPLIIKADVQGSQEALIHALTKLSTDEVRVQVVHAAVGGISESDVNLAAA
                     SSAVIIGFNVRADAQARKVAETEGIDIRYYNIIYDAVDDVKAALGGMLSPERRETSLG
                     LLEIRQIIRVPKVGNIAGCRVLEGVVRRNASVRLLRDNVVIWTGELASLKHFKDDVRE
                     VQAGQECGLSLKGYDDIRELDQLEIFEVTEVARTL"
     gene            7565..7930
                     /gene="rbfA"
                     /locus_tag="ACZPB6_01420"
     CDS             7565..7930
                     /gene="rbfA"
                     /locus_tag="ACZPB6_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR1183169.1"
                     /translation="MKPKKPGRSLRVADQIARDLAVLIPKEVRDPRVGLVTVTGCEIT
                     PDYAHAKVFFTVIGSDPAVCEEGLNAAAGMLRNHLFRKLTIHTVPTLHFAHDDSVNRG
                     FEMDALIRKAMSETRPEEN"
     gene            7932..8825
                     /gene="truB"
                     /locus_tag="ACZPB6_01425"
     CDS             7932..8825
                     /gene="truB"
                     /locus_tag="ACZPB6_01425"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR1183170.1"
                     /translation="MARRGDPIDGVMLLDKPLGMSSNAALQTVRRLVNAQKAGHTGTL
                     DPMATGLLPLCFGNATKFSADLLHAEKGYVARVKLGEVSSTGDAEGEIVERHPVDVTA
                     EALEEAVAAFLGEIVQIPPMYSALKVNGKCLYQLARQGETVERAPRTVTVREIAASDF
                     DGTSFTLTCLVSKGTYIRVLAEDIGRRLGCGAHLTALRRTRVGDLSIDDAVTVETLEE
                     CGSPEEARRLLKGADSLLQTFEAVTLDEMQTARFMTGQRLALGLTMRGRVRVYGPNNM
                     LLGTARANERGVLEPERLIAH"
     gene            8843..10699
                     /gene="typA"
                     /locus_tag="ACZPB6_01430"
     CDS             8843..10699
                     /gene="typA"
                     /locus_tag="ACZPB6_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR1183171.1"
                     /translation="MTRAIRNIAIIAHVDHGKTTMVDCLLRSAGTFRANQQVAERVMD
                     SNDLERERGITILSKNCAVEYEGTHINIIDTPGHADFGGEVERVLSMVDGVLLLVDAV
                     EGPMPQTRFVTRKALAQGLKPIVVVNKIDRPGARPDWVVNQTFDLFDKLGATEDQLDF
                     PVIYASALNGFAGHTTDVEELKQIGNMRAVFDDIIKYIPVRDDDPDGPLMLQICSLDY
                     SSYVGKIGIGRVHRGRIRPNMDVAIMNGPEDTPKRVKINQVLKFEGLERQLVDEAEAG
                     DIVLVNGIEELSIGTTITDLQQPEALPMLKVDEPTLTMNFQVNSSPLAGREGKFVTSR
                     QIRERLERELKSNVAMRVRNTDDETVWEVAGRGELHLTILLENMRREGFELAVSRPRV
                     LLKEVDGVKMEPYELLTVDVEEEHQGSVMEELGRRKGDLQDMQPDGKGRVRLEYRIPA
                     RGLIGFQSLFMTMCRGTGIMSHVFDDYGPISRGDIGERRSGVIISQDDGDAVAYALWK
                     IQERGRLFVSPGDKLYEGMIIGEHARENDIVVNPIRGKQLTNIRASGTDEAIRLVPPV
                     KLTLESAVEFINDDELVEITPHSIRLRKRYLKDFERKRAARAVQNEFGEKDE"
     gene            10692..11096
                     /locus_tag="ACZPB6_01435"
     CDS             10692..11096
                     /locus_tag="ACZPB6_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HIR1183172.1"
                     /translation="MSKKKSKACDAACPCGSGLALAACCGRLHAGVPAETPEALMRSR
                     YSAYALDLDAYVLETWAPETRPAVLFEDGEERPKWLSLVIHSSYEEGAEGRVSFTARG
                     RTSQGAFRLEENSRFRLENGRWLYVDGTFDDR"
     gene            11172..12158
                     /gene="dusA"
                     /locus_tag="ACZPB6_01440"
     CDS             11172..12158
                     /gene="dusA"
                     /locus_tag="ACZPB6_01440"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HIR1183173.1"
                     /translation="MPLNPGDWRFTVAPMLDWTDRHCRYFHRLLTSRARLYTEMVTTG
                     AIIHGDRDRLLGFSQPEHPVALQLGGSDPADLAESVRIASAYGYDEYNLNCGCPSERV
                     QKGSFGACLMLEPQTVAACVRAMQDVTDKPVTVKHRIGVDDHNEYGFVRDFVGTVYDA
                     GCRVFIVHTRSAWLKGLSPKENREVPPLVRETAFRLKKDFPDTVMLINGQIASLDEAK
                     ALVDAGMDGVMVGRAAYQNPWMLAGADEVLYGVEHDVTRLECVHRMTAYLEENYRDDV
                     HAIRAVARHVNGLAQGLPGARRWRQVLSDPAAIKDRGAGLIQYAWEEAFGEG"
     gene            complement(12217..13518)
                     /locus_tag="ACZPB6_01445"
     CDS             complement(12217..13518)
                     /locus_tag="ACZPB6_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183174.1"
                     /translation="MSVHITRRALIGSASAAAALWLTGCATTAAPREENIRPATFSNL
                     GARAAPATPLTAKRIPMPVVDAFKGDGIFFFDDDEGAQFRITDLIRENNRYKVEEDYA
                     FGKPHGFSWGSGAVGIMRDGRRVPPENPTGLRPEGDHVVIEFDGREKLELRVTLEAYD
                     VSGQEIRDFLRTRGNMPTKAALFMGEERFPAGSVAYAATLWIIQDELLALSHTAFTGS
                     NNIEDFSERFTKETPYCLNVLPGDGVTPVGLRFEKTIKKKTGKEKIKTRRGTKTRTVE
                     LPQNGLVNVYHTKSGTIFCQRSKPDPVVKARWDLRYLNGTRTLTVDFPTLIDSLSIGL
                     MTANRGKLMPAFAEELTPTSRKTRVIPTAVWLKNERIRDSQYRFNKTAATAVKAAIEA
                     SRPRRKAWEAANESDVTRRARALQAKKAASGKARRTPAKKR"
     gene            complement(13669..14667)
                     /locus_tag="ACZPB6_01450"
     CDS             complement(13669..14667)
                     /locus_tag="ACZPB6_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183175.1"
                     /translation="MPRSVRFDNGKALVPLGLIAERWIRDYWQLASGPRMPRQIHQNR
                     ELGFGTALERTMTKCHSQYGVFESLMRNEARSKDQTSLLQLLFDDGVTTALKGPIHYI
                     QDDGRNPVFTYVATHAGRRPPFTGRGSIIERYGLLAFPAELWLELNRIAPWLEDSLIL
                     GWARLSARFESLCSAEHALTQADIVVRLLPPESARDTDFTASAYKAAISRGSLRSVWS
                     GTPLKAQTLAVNHMLPWARFHCNDLWNLMPTDRVENGRKSDAIPSADILHDSRDKIFS
                     NWALLSSLAPTRFASEAEIALTRTPLPKLHWETPLFDALLETADMAARQLQSARWP"
     gene            complement(14803..15396)
                     /locus_tag="ACZPB6_01455"
     CDS             complement(14803..15396)
                     /locus_tag="ACZPB6_01455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1183176.1"
                     /translation="MPMPSAYETRSEAFFRLYEGETPQEIQTLLTRWIPKGSRVLELA
                     CGSSRDARFMSGLGAIVEATDGSGPLLKLAEARAQALLGAESPSFSLLCLPPDKGSED
                     ALFARLPRFDAVYTCGVLQHLSDHELYEGACFMERAVTDKGTLIVVVPLDHKGDPDRK
                     TFLRDSLDYATLFERMGFRLASQEIRDGVGSPGHECR"
     gene            complement(15407..16828)
                     /locus_tag="ACZPB6_01460"
     CDS             complement(15407..16828)
                     /locus_tag="ACZPB6_01460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR1183177.1"
                     /translation="MPAYQEIIDALRSAVSDTGLITDPADISAAALDGRGRRQGEALA
                     VVRPASTEEVSLVMKTAAAYGLSVIPQGGNTSNVGGATPEPGASAETARRTLILQLGH
                     MKRILNVDTVNNTITLEAGVVLQDAQKAASDAGRLLPLSLAAEGSCQTGGVIAANAGG
                     VHVLRYGMARRQVLGLKVVLASGEVLDLMKGLRKDNSGYSLRDVFIGSEGTLGVITEA
                     VLALEPMPRSIVSAWITPHRLEDVETLFETLEAAFGPGLTAFELIGRTPLESLSSVTG
                     MTPPVSLSDWQVLLDVSDWRRDSDESSEELEGVLAPLLENGLILDGAVSRSESDREAF
                     WRCRETIPSAVKREGGNVKHDISVPRSSLVRFIRETSAALEETFPGVKPSVFGHYGDG
                     NLHFNVGPAGVAFPHEEAVHRLVHDRVLAEGGSIAAEHGVGSLKTQELKRTKTPEELA
                     AMRALKLAFDPDNRLNPGRIVEI"
     gene            complement(16961..17614)
                     /gene="adk"
                     /locus_tag="ACZPB6_01465"
     CDS             complement(16961..17614)
                     /gene="adk"
                     /locus_tag="ACZPB6_01465"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - AMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR1183178.1"
                     /translation="MRLILIGPPGAGKGTQAKFICEKFGIPQISTGDMLRAAVKAGTP
                     LGLAAKAVMDKGQLVSDDIIIGLVKDRLTQPDAVNGALFDGFPRTIPQAEALQAAGIP
                     IDFVLEIQVPDAEIVERMSGRRVDPVSGRTYHIKYNPPKVEGKDDVTGDPLIQRDDDR
                     EEVVRKRLDVYHSQTEALVGFYSDLAKSGDKTAPQYRAISGLGAIDAITARVFEALK"
     gene            complement(17754..18524)
                     /gene="kdsB"
                     /locus_tag="ACZPB6_01470"
     CDS             complement(17754..18524)
                     /gene="kdsB"
                     /locus_tag="ACZPB6_01470"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR1183179.1"
                     /translation="MSFTVIIPARLKSSRLPDKPLADICGRPMIVRVAERALSCGADR
                     VVVAVDDASVYEACAAAGLDVIMTDPHHPTGTDRLAEAVAKIGLADDEIVVNLQGDEP
                     LMPLNVIRDVADLLASRPDCAMATAAHTIHDVESFFNPNVVKVVLDDRGTALTFSRAP
                     IPWPRDHFRETKESLPEGFAPLHHLGLYAYRAGFLRRFPTLPQAPLEKLESLEQLRAL
                     YYGERIAVMVLDAALPAGVDTQKDLDRVRAIFEKSAPV"
     gene            complement(18532..18750)
                     /locus_tag="ACZPB6_01475"
     CDS             complement(18532..18750)
                     /locus_tag="ACZPB6_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR1183180.1"
                     /translation="MSAKLNDMIVCPVCKGRLLQGDGERRELVCPRCRLAFEVRHDIP
                     VMLRNAARSLSDNEARHYSERSAALDPK"
     gene            complement(18886..19920)
                     /gene="lpxK"
                     /locus_tag="ACZPB6_01480"
     CDS             complement(18886..19920)
                     /gene="lpxK"
                     /locus_tag="ACZPB6_01480"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR1183181.1"
                     /translation="MTWRAGLEAWAHRQWASRGPVAWLLSPLSLVFLAVSRSRRERTV
                     PNRLPVPVVVVGNIYVGGTGKTPVTIALVRELQARGWRPGVISRGFGRREDGIRLINE
                     ETPAAEAGDEPLLIARETGVPVAVGRARFEAGRALLEAHPEVDLIVSDDGLQHAALAR
                     DVEIAVVGARGLGNGWVLPAGPLREPPSRLDTVDAIVLNTSNEAVVESRTPRFAASSC
                     FGACTQLATGRTALIDELADRIHAEKLKPLSAAGIAAPGRFFAMIRAHDIEGAELELG
                     DHFDFAENPFANRTEDIIFITGKDAVKCRHDPALAGDKRIWVVGLEVKLDSYLIDLID
                     KRARDAAGRH"
BASE COUNT         3890 a         6152 c         6642 g         3726 t
ORIGIN      
        1 ttattttggc gccgcctttt tctacaatgg gcgctgtttc agataccctt tcgtccgtct
       61 ctcatgactc cgcagcacat cattgaagca gcgctactga gctcgcggcg gccgatgccg
      121 gtgcgcgaga tcaggcgtct cttcgacgac aggctgagcg ccaagtccgt gaaggaacac
      181 ctcgctgagc ttcaggcctt ctgggaggac cgcggcatgc gcctcgtgga gctctccgac
      241 gggtggcgct tccagacggc ctcggagctt ggtcccagat tcctgcgcct tgacgaggaa
      301 aagccgcccc gctattcgag ggcggccatg gagacgctgg ccatcatcgc ctatcatcag
      361 cccgtgaccc gcggcgacat tgaggagctt cgcggcgtga ccgtcaatcc ggccaccctc
      421 aggctcttcg aggagcgcgg atggatcgag acggtgggtt atcgggagtc gccgggcagg
      481 ccggcgcttc ttgccacgac cagaactttt ttaaatgatc tcggactcag aagccttgca
      541 gaactgccgg gccctgatgc cgagccgctt cccgacttcc tgctgtcgga agccctgccg
      601 ccttcggcgg cagccgacga agggccgatg ccgcttcaga aggagattga ttttgagaaa
      661 caacagtaat tctcgcggcg ttcgccgtcc gcagtccgcc gaacgcaagc gcacgccgtt
      721 ccgtcgtcct ggcgaaggca atttgcctcg cacccgtcgc gtgcgcgagg acgccccctt
      781 ttccgagcgc gcaggcctgg cctctccgcg cgaggccttc ggacagatgg agtccgaggc
      841 cgacggctac aggggctttg cggatcgtcc gatcgcgctc gacatcatcg accgcgcccg
      901 ccagcagcag acgcctgccg atctgccgac cgaaaagctt cagaaggcgc tcgccgatgc
      961 tgggctcgga tcccgccgcg acatggaggc tctgatcgcc tccggcgtcg tgaccgtcaa
     1021 cggtcaaacc gccaccgtgg gtgaccgcgt tacggctgcg gacatgattc gtgtcgaggg
     1081 caggctcgtg cgccgcacga tggctgcctc ctccacgccc cgcgttctga tgtaccacaa
     1141 gatggccggc gaaatcgtct cccgtgacga ccctgaaggc cgtccctccg tctttgcgcg
     1201 tcttccccgc gtctcgggcg cccgctgggt ggccgtgggc cgtcttgact tcaataccga
     1261 gggccttctt ctcttcacga cctccggcgc gcttgccaac cgccttatgc atccccgtta
     1321 cgagatcgat cgcgagtatg ccgttcgagt catcggcgag cttgaggccg agcagatgct
     1381 tcagcttcag cagggcattg agcttgagga cggcatggcg aagttcgaca agctcgagga
     1441 tcaaggcgga acgggcatga accactggta cctcgtgcag atccgcgagg gtcgcaatcg
     1501 cgaggtccgc cgcatgttcg aagctgtcgg catgacggtt tcgcgcctta tccgcgttcg
     1561 ctacggcgcc gtttctcttc cgaagaccct gccgcgcggc aaacgcatgg aactcacgcc
     1621 tgaagaggtt cgcgcctgga tgcttgacct cgatgaggcc gagaagaaaa tggccgccgc
     1681 agctcctgca aaggctgagg ctaagaaggc cgaaaaggcc gccgctcgtg aaaaggcccg
     1741 cgcccgcgct cgtgccgaaa gcgaggccga ggcccgcgag gctcgtcgag gcacccgctt
     1801 caaggaaggc aagtccgacc gcacgggcgt gcccatgtgg gaggctcgcg acatgagcga
     1861 tcgcccgacg cagcgccgcc gaacgggcga acgctcctga tcgcgaaagg acctgaaaaa
     1921 gcggctccgc gcttgcgcgg ggacgctttt tcacctacaa tcgcgggtgt tcgtgaaaac
     1981 ccgaggcgac cttcaagatc gtgtccgtcc ctgagcaaac gggggagagg gcgttttcac
     2041 ggaaaggttc aggcggcgca gaccgagaga aggccgcgcc gttgccctgc cggaatgaga
     2101 atcccgaggc cgcgtgtcca gaaggacatg cagaaggctg cagacggacc gccggcgtca
     2161 gagggtgtcg aatgggcgtt tgcccatttt tttttgtttt tttcgagctt cggctgactt
     2221 ttgtccgctt aagctgcagg gacgagccgt tcagcacgga cgcgccgtca ggacgggccg
     2281 ggatgactcc ggccgcaatt taataggaag tttgatcaat gacccaatcc ggagcgctta
     2341 acgagctcgt tgaacgtacc gtcgaaggca tgggctatga gttcgtcgaa ctcgagcgcc
     2401 ttgccagagg ccttgtccgc gtgacgatcg acaccaccgc cgaaggcggc atctcgcttg
     2461 aggactgcga acgcgtgagc gatcagctca cccacctctt cacggtggag gacattccct
     2521 atgagcgcct tgaggtgtcc tctcccggcg ttgagcgccc cttgaagcgc gcccgcgact
     2581 gggatcgctt cgccggcgaa ctcgctcacg tcgagctctt tgcgcccctt cacgccgaag
     2641 gcttccctga ggccggccgc cgcaagctcg atggccgcat catctccatt gaaggcgagt
     2701 ccggtgctga aacgatcacg ttcgacttct tcgaggtctt catcgcccgc acgcccactg
     2761 ctgccgttac ggcgctccgc ggccgcagga agaagacggc cgaggttccc cccgtgcgcg
     2821 tcaccttccc gctgtccgat gtcgacaatg cccatcttct tgctgaattg aactttaaag
     2881 gttgatttca atgaactgtc gtgaaatgct ccagcttgtc gacgtactcg caagcgaaaa
     2941 gaatgttgcc aaggacaccg tcttcggcgt gctcgaggcc gcgcttgcca gcgccgtcaa
     3001 gaaggctcag ttccccggtg aggacgccga cgtcgtcgtg catgtcgacc gcgtgacggg
     3061 cgaattcaag gccgcccgcc gctggctcgt cgttgccgac gaggaaggtc tccaggagcc
     3121 ggaccgtcag gaaatgtatt ccgacgtcat cgacgaatat ccggaaatcc agcccgggga
     3181 attcatcgag cgcgagatcg aagtgattga cgtcgacacg acgggccgcc gcttcgctca
     3241 ggacgccaag caggtcatcc ttcagcgcct gcgtgacgcc gaacgcgagc agatcctcaa
     3301 ggagttcctc gatcgcaacg aaagcatcgt gactggcacg atcaagcgca tcgacaaggg
     3361 cgacgccatc gttgaaatcg gccgtcttga agctcgcctg ccgcgcaacc agatgattcc
     3421 gcgtgagaat ctccgcacgg gtgaccgcgt gcgcgcctac gtcgaccatg tcggcgagtc
     3481 caacaagggc cagcaggtgt tcctctcccg cacgtccccc gaattcatca agaagctctt
     3541 cgagcttgaa gtcccggaaa tcgaggaagg cctcctcgag atcaagtccg ccgcccgtga
     3601 tccgggcgtg cgcgccaaga tcgccgtcta cgcccgcgac cgccgcatcg atccgatcgg
     3661 cacctgcgta ggcatgcgcg gctcccgcgt caatgccgtg acgaacgaac tcggcggcga
     3721 acgcgtcgac atcgtgccct ggaacgagga tcccgcccag ttcgtggtcg gtgcccttga
     3781 gcccgccaag gtccgttcga tcgtcatgct cgaagagacc cacacgatgg aagtcgtcgt
     3841 cgacgaggac aatctcgcca tcgccatcgg ccgcgccggc cagaacgttc gtcttgcgtc
     3901 cgagctcacc ggctggcaga tcaacatcat gaccgaagct gccgccgctc agaagcgcga
     3961 tgaggaagtc gcgaagatcc gcgccgactt catggacagc ctcgacatcg acgaggccgc
     4021 cgccgacgtg cttatcggtg aaggcttctc ctccgttgag gaaatcgctt acgttcctga
     4081 gaaggagctc tacgcgatcg aggccttcga ccgtgaaacg gtcgacgagc ttcgccagcg
     4141 cgcccgcaac aagcttctcg ccgacgccat cacccgtgaa gagaatctgc gcaaggccga
     4201 cgagccgttg ctgagccttg agggcatgga caacgatctc gccagccagc ttgtgggcca
     4261 cggcgtcaag acgcttgacg atcttggcga actcgccacg gacgatctcg ttgaaatgac
     4321 gggcatcgac gaagagcgcg cgagcagcct catcatgaag gcccgcgagc actggttcga
     4381 ataatcaggc gcaaggcgaa gggagcgcga ccgcgaatgg cggagaccgc gctccggacg
     4441 aatcaggatt tgtccccaaa gaaatggtct tccgccgcga atgaataatc gccgtgtcgt
     4501 ctgcgcgcga acgtccttcg ggacgcaggt gtacagacgc aggcacggca gatgcgttcg
     4561 gtccgggaaa gtcccgcagc gtcccagagg acgcagggca ttcaggctga aggcatcaag
     4621 gagaaaggaa tttatggcca gcaaaacggt aagcgaattc gcacaggaac tcaaggtggc
     4681 cgccaagacg cttctggctc aacttcgagc cgcgggcgtc aacaaggcga gcgaaacgga
     4741 tgaattgagc gagaccgaca agacgcgcct gctcgacagc ctgcgcgtcg agcgcacgca
     4801 atcggctcag cgcaagatca ccgtcacgcg caaggaacag agcgtcgtca agcagcagga
     4861 cggctccggc cgctcccgtg cgattccggt cgaagtgcga cgcaagcgcg tcttcgtgaa
     4921 gaatccgcag gtgctcgccg aggaggctgc tcgcgctgaa gccgaagccg ctcaggcccg
     4981 ccgcgccgcc gaggaaaagg ccgccgccga agcctgcgcc cgtgcggagg ctgaggagcg
     5041 cgcccgtgct gaagaggccc gccgcgccga ggaagcccgc atccgcgagg aagaggagcg
     5101 ccgcgccgcc caggaacgtg ccgaagccgc acgccgtgaa gctgaagaag cccgtgctcg
     5161 tgaggaagct ttgaagaagg aacaggaaaa ggacgaggcc gccctcaagg ccgcccgtcg
     5221 cgaggccgaa aaggccaagc ttgatgctga agtggccgcc gccactgcca agcgcatgga
     5281 ggccgaggcc gccgaacgcc gtcgccgcga ggaagctcgc cgcaaggccg aggaggaggc
     5341 ccgcagcatc cgcgccatga tgaaccgcaa gaacggagtt ctcaccgcca agaaggtcaa
     5401 gcccgctgaa aagcccgccg aagccccgaa ggccgctgag cagaagcctg ccgccaagga
     5461 tgacaaggcc aagaagaccg ctcgtccgca gcgtccggcc gccgagcctc agcagaatgc
     5521 cggtgatggc cgcaagggcc acccgtccga ccgcaagaag ggcggcaagg gcggctccga
     5581 ccgcttcggt gatgagggtc agcatcgccg cacgatcaag acgcgcggtg aggacgattc
     5641 cgggcagacc ggctggcgca cggcccgcgg ccgtcgcagc caggaccgca accgtcatgc
     5701 cgaacccgtt cagcaggttc aggaacaggt ggtgcgtgaa gttcaggtgc ctgagacgat
     5761 cagcgttgcg gaccttgcct acaagatggc cgtcaaggcc accgaagtga tcaagaccct
     5821 catgaagatg gggcagatgg tcacgatcaa ccagatgctc gatcaggaca cggcgatgct
     5881 catcgtcgag gaaatgggcc acaaggccgt cgccgccaag ccggacgatc ccgaagccat
     5941 cctcggtgaa ctgcagaacg tcgaatatcc cgctctgccg cgtccgcctg tcgtcacgat
     6001 catgggccac gttgaccacg gcaagacctc gctgctcgac tacattcgtc gtgccaaggt
     6061 tgcggccggt gaagccggcg gcattacgca gcacattggt gcctaccatg tgaagacgcc
     6121 gcgcggcatc gtcaccttcc tcgacacccc gggccatgaa gcctttacgg ccatgcgcgc
     6181 ccgtggcgct caggccaccg acgtcgtcat tctggtggtt gccgccgacg acggcgtgat
     6241 gccccagacg aaggaggcca ttgcgcactc ccgtgccgct ggcgttccgc tcgttgtcgc
     6301 catcaacaag atcgacaagc ccgaggccaa tcctgagcgc gtaaagcagg agctcgttgc
     6361 caacgacgtc atgcctgaag agtggggcgg cgacgtgccg ttctgccccg tgtccgccaa
     6421 gacgggcgca ggtgttgacg aacttcttga aaacgtgctg cttcaggccg aaatgctcga
     6481 gctcaaggct ccggtcgaag gtcccgccca cggcatcgtg atcgaatccc gcctcgacaa
     6541 gggccgcggc cccgtcgcgt ccattctggt tcagggtggt acgctgcatc gcggcgacat
     6601 cgtgctcgtc ggccaggaat tcggccgcgt gcgcgccatg atcaacgaac tcggcaaggc
     6661 ccagcaggcc gccggtccgt ccattcctgt cgaaattcag ggcctttccg gcgttcctgc
     6721 cgcgggtgac gaggtcatcg ttctcaacga cgagcgcaag gcccgtgaaa tctcgctctt
     6781 ccgtcagggc aagtaccgcg atcagaaact cgccaaacag catgcgacga acctcgccaa
     6841 cctcttctcc ggtgcgaacg agggcgaagt caagacgctg ccgctcatca tcaaggccga
     6901 cgtccagggc tctcaggagg cgctcatcca tgcgcttacg aagctctcca cggacgaagt
     6961 ccgcgttcag gtcgttcatg ccgccgtcgg cggcatcagc gagtccgacg tgaatctcgc
     7021 cgctgcgtcg tccgccgtga tcatcggctt caacgtccgc gccgacgctc aggcgcgcaa
     7081 ggttgccgag accgaaggca tcgacatccg ctactacaac atcatctacg acgccgtgga
     7141 tgacgttaag gccgctctgg gcggcatgct ctctcccgaa cgccgcgaaa cgtctctcgg
     7201 ccttctcgag atccgccaga tcatccgcgt gccgaaggtc ggcaacatcg ccggctgccg
     7261 cgtgctcgaa ggcgtggttc gccgcaatgc gtcggtccga ctgcttcgcg acaacgtggt
     7321 catctggacg ggcgagctcg cctccctcaa gcacttcaag gacgacgtcc gcgaagttca
     7381 ggccggtcag gaatgcggcc tgtcgctcaa gggctacgac gacatccgtg agctcgacca
     7441 gcttgagatc ttcgaggtga ccgaagtcgc ccgcacgctt taagaggatc gttccaacat
     7501 caggggagga catgccttcg ggctgtcctc ccgtcattga ctttatgcaa ggggggcaac
     7561 cttcatgaaa ccgaagaaac ccggccgttc cctccgagtg gccgaccaga tcgcccgcga
     7621 tctcgccgtg ctcatcccga aggaggtccg cgatccccgc gtcggcctcg ttacggtgac
     7681 gggctgcgag attacgcccg actatgcgca tgccaaggtc ttctttacgg tgatcgggag
     7741 cgatcccgcc gtctgtgagg aaggtctcaa tgccgcagcg ggcatgcttc gcaaccatct
     7801 ctttcgcaag ctgacgatcc atactgtgcc gacgcttcac ttcgcgcacg acgacagcgt
     7861 gaaccgcggc ttcgaaatgg acgccctcat ccgcaaggcc atgagcgaaa cgcgtcccga
     7921 ggagaactga cttggccagg cgtggagatc cgattgacgg cgtcatgctc cttgacaagc
     7981 cgctcggcat gagttcgaac gctgcgctcc agacggtgcg caggcttgtc aacgcgcaga
     8041 aggctggcca tacgggcact ctcgatccga tggccacggg gcttctgccg ctttgcttcg
     8101 gcaacgcaac gaagttttcc gccgacctgc tgcacgccga aaagggctat gtcgcccgcg
     8161 tgaagctcgg cgaggtctcg tccacgggcg atgccgaggg cgagatcgtc gagcgtcatc
     8221 ccgtcgacgt gacggccgag gcgctcgaag aggccgtcgc cgcctttctg ggtgagatcg
     8281 ttcagattcc gcccatgtac tcagccctca aggtcaacgg caagtgcctt tatcagctcg
     8341 cccgtcaggg tgagacggtt gaacgtgctc cgcgcacggt caccgtgcgc gagatcgcgg
     8401 cctccgattt tgacggcacg tccttcacac tcacgtgtct cgtttccaag ggtacgtaca
     8461 ttcgcgtgct tgccgaggac atcgggcgca ggcttgggtg cggcgctcat ctgacggctc
     8521 ttcgccgcac gcgcgtcggc gacctttcta tcgacgacgc cgtgacggtt gagacgcttg
     8581 aagaatgcgg ctctcccgaa gaggcccgcc gtcttctcaa gggggccgac tctcttctgc
     8641 agacctttga agccgtgacg cttgacgaaa tgcagacggc acgcttcatg acgggacagc
     8701 gccttgccct cgggcttaca atgcgggggc gcgtccgcgt atacggaccg aacaacatgc
     8761 tgctcgggac ggcccgcgca aacgagcgcg gcgtccttga gcctgaaaga ctcatcgcac
     8821 attaaatcaa cacgagaaaa aaatgactcg agctattcgt aacattgcga tcatcgcgca
     8881 cgtcgaccac ggcaagacca cgatggtgga ctgcctgctt cgcagcgccg gcaccttccg
     8941 cgccaatcag caggttgctg aacgcgtgat ggactccaac gatctggaac gcgaacgcgg
     9001 catcaccatt ctctcgaaga actgcgccgt cgaatacgaa ggtacgcaca tcaacatcat
     9061 tgacaccccg gggcatgctg acttcggcgg tgaagttgaa cgcgtcctct ccatggtcga
     9121 cggcgtgctg ctcctcgtcg acgccgttga aggcccgatg ccgcagacgc gcttcgtgac
     9181 ccgcaaggct ctcgcccagg gcctcaagcc catcgtcgtc gtcaacaaga tcgaccgccc
     9241 gggcgcccgt cccgactggg tcgtcaacca gacgttcgac ctcttcgaca agctcggtgc
     9301 cacggaagac cagctcgact tccccgtgat ctacgcctcc gctctcaacg gctttgccgg
     9361 tcacacgacc gacgtcgaag agctcaagca gatcggcaac atgcgcgccg tcttcgacga
     9421 catcatcaag tacatccccg tccgtgacga cgatcccgac ggccccctga tgcttcagat
     9481 ctgctcgctc gactactcga gctacgtggg caagatcggc atcggccgcg tgcatcgcgg
     9541 ccgcatccgc ccgaacatgg acgtcgcgat catgaacggt ccggaagaca ccccgaagcg
     9601 cgtgaagatc aatcaggtcc tgaagttcga gggtctcgag cgtcagctcg tcgatgaagc
     9661 cgaagccggc gacatcgtgc tcgtcaacgg cattgaagag ctctccatcg gcacgacgat
     9721 caccgatctt cagcaacccg aagccctccc gatgctcaag gtcgatgagc cgaccctgac
     9781 gatgaacttc caggttaact cctctccgct cgccggccgt gaaggcaagt tcgtgacgtc
     9841 ccgccagatt cgcgaacgtc tcgaacgcga gctcaagtcc aacgtcgcca tgcgcgtgcg
     9901 caacaccgac gacgaaaccg tctgggaagt cgccggtcgc ggtgaacttc atctcacgat
     9961 tcttctcgag aacatgcgcc gcgaaggctt cgaactggcc gtctcccgtc cgcgcgtcct
    10021 tctcaaggaa gtcgacggcg tcaagatgga accgtacgaa cttctgacgg tcgacgtcga
    10081 ggaagagcat cagggctccg tcatggaaga gctcggccgc cgcaagggcg acctgcagga
    10141 catgcagccg gacggcaagg gccgcgtccg ccttgaatac cgcattcccg cccgcggtct
    10201 catcggcttc cagagcctct tcatgacgat gtgccgcggc accggcatta tgagccacgt
    10261 cttcgacgac tacggtccca tcagccgcgg cgacatcggc gagcgccgct ccggcgtgat
    10321 catctcccag gacgacggcg atgccgtggc ctatgctctc tggaagattc aggagcgcgg
    10381 ccgcctcttc gtctccccgg gcgacaagct ctacgaaggc atgatcatcg gcgagcacgc
    10441 ccgtgaaaac gacatcgtcg tgaacccgat ccgcggcaag cagctcacca acatccgtgc
    10501 ctccggtacg gacgaagcca tccgtctggt gccgcctgtc aagctcacgc tggaaagcgc
    10561 cgtcgagttc atcaatgacg acgaacttgt cgaaatcacg ccgcactcca tccgcctgcg
    10621 caagcgctat ctgaaggact tcgaacgcaa gcgcgccgcc cgcgccgttc agaacgaatt
    10681 cggcgagaag gatgagtaag aagaagtcga aggcgtgtga tgcggcctgt ccctgcggga
    10741 gcgggctcgc actcgcagcc tgttgcgggc gtcttcatgc gggcgtgccc gcagaaacgc
    10801 ccgaggctct gatgcgaagc cgctattcgg catatgctct cgatctcgac gcctatgtcc
    10861 tagagacgtg ggcgcctgaa acgcggcccg ccgtcctttt cgaagatggc gaggaacggc
    10921 cgaagtggct ttccctcgtc attcactcct cttacgagga aggggcggag ggcagagtca
    10981 gcttcaccgc gcgcggcagg acctctcagg gcgccttcag gttggaggag aacagccgct
    11041 tccgtcttga gaacggccga tggctatatg tcgacggcac gtttgacgac aggtaagatc
    11101 tcgacttatg agccgccctt cggggcggct taatttttat tcaacaacac ttgtttacgg
    11161 aaccgacttt catgcctctc aatcccgggg actggcgctt caccgtggcg ccgatgctgg
    11221 actggacaga ccggcactgc aggtactttc acaggctgct cacgagcagg gctcgcctct
    11281 acaccgagat ggtgacgact ggggcgatca ttcacggcga tcgtgaccgc ctgctcggct
    11341 tttcacagcc ggagcatccc gtcgcactgc agttgggcgg ctcggatcct gccgatctgg
    11401 cggagtccgt ccgaattgca tcagcttacg gttatgacga atacaacctc aactgcggat
    11461 gcccttccga gcgtgtgcag aagggaagct tcggggcgtg tctgatgctc gagcctcaga
    11521 ccgtggccgc ctgcgtcagg gcgatgcagg acgtgacgga caagcccgtg acggtgaagc
    11581 accgcatcgg ggtggatgat cacaacgaat acggctttgt gcgcgacttc gtgggcacgg
    11641 tctacgatgc tggttgccgc gtcttcatcg ttcatacgcg ctcggcctgg ctcaaggggc
    11701 tgtcgccgaa ggagaatcga gaggttcctc cgcttgttcg cgagactgcc ttcaggctca
    11761 agaaggactt tcccgacacg gtgatgctga tcaatggaca gatcgcctcc ctcgacgagg
    11821 cgaaggcgct tgttgatgca ggcatggacg gcgtgatggt gggacgcgcg gcctatcaga
    11881 atccctggat gctcgccggc gccgacgagg tgctctacgg tgttgagcac gacgtgacgc
    11941 gccttgaatg cgttcatcgc atgacggcgt atctggagga gaactacagg gacgacgtgc
    12001 acgccatccg tgccgtggct cgtcatgtga acggtctggc tcaggggctg cccggcgcac
    12061 gcagatggcg tcaggtgctt tccgatcctg cggccatcaa ggacagggga gcggggctca
    12121 ttcagtatgc atgggaggag gccttcggcg aaggctgatg acggcatgaa aaacgccagg
    12181 gtgaaagctc tggcgttttc gtacgcgaaa ggatgatcag cgcttcttgg cgggcgtcct
    12241 gcgtgccttg ccagaggcgg ccttcttggc ctgaagggcg cgggcgcggc gcgtcacgtc
    12301 gctttcattc gcggcttccc aggccttgcg gcgcgggcgg gaggcttcga tggcggcctt
    12361 gacggccgtc gctgcggtct tgttgaagcg gtactggctg tctcggatcc tttcattctt
    12421 gagccagacg gcggtgggaa tgacgcgggt cttgcggctc gtcggcgtga gctcctcggc
    12481 gaaggccggc atgagcttgc cgcggttggc agtcatgagg ccgatgctca gggagtcgat
    12541 gagcgtggga aagtcgaccg tcagcgtgcg ggtgccgttg aggtagcgaa ggtcccagcg
    12601 ggcctttacg acggggtcgg gcttgcttcg ctggcagaag atcgtgccgg acttcgtgtg
    12661 atagacgttg acgaggccgt tctgcggcag ttccaccgtg cgggtctttg ttcctcggcg
    12721 cgtcttgatc ttttccttgc cagtcttctt cttgatggtc ttttcgaagc gaaggcccac
    12781 gggcgtgaca ccgtcgccgg gaagcacgtt gagacagtat ggcgtttcct tcgtgaagcg
    12841 ctcggagaag tcctcgatgt tgttcgaacc cgtgaaggca gtgtggctca gcgcgagcag
    12901 ctcgtcctga atgatccaga gcgtggcggc ataggctaca ctgcctgcgg ggaatcgctc
    12961 ctcgcccatg aagagcgcgg ccttggtcgg catgttgcct cgggtgcgca ggaagtctcg
    13021 gatttcctga ccggagacgt cgtaggcctc cagcgtgacg cgaagctcga gtttttccct
    13081 gccgtcgaat tcgatgacga cgtggtcgcc ctcggggcgg agtcctgtcg gattttcagg
    13141 cggaacgcgt cggccgtctc tcatgatgcc gacggcaccg gaaccccagg agaagccgtg
    13201 aggcttgccg aaggcgtagt cctcttcgac cttgtagcgg ttgttttcac ggatcaggtc
    13261 ggtgatgcgg aactgcgcgc cttcatcatc gtcgaagaag aagatgccgt cgcccttgaa
    13321 ggcatcgacc acgggcatgg gaatgcgctt ggcggtgaga ggcgtggcgg gggcggcgcg
    13381 ggcgccgaga ttgctgaagg tggcggggcg gatgttttcc tcacgggggg cggcggtggt
    13441 cgcacagcct gtgagccaga gtgccgcagc ggcggaagcg gaaccaatca gcgcgcggcg
    13501 cgtgatgtga acagacattg ggggagtcct cacagagttt gcagtgtttg tgcgcaggcg
    13561 tgcgaaatgc atcttctgtt gcctatgggc gacggaaggc gaaggcctgt cggatgagga
    13621 tagcagatcg ggtgacgggc ggacgccgtt ctcaggtaag gaggcgtttc agggccatcg
    13681 ggcggactga agttgtctcg ccgccatgtc ggcggtttcc agaagtgcgt cgaaaagcgg
    13741 ggtttcccaa tgcagtttgg gaagcggcgt tcgggtgagc gctatttcag cttcactggc
    13801 gaagcgggtg ggagcgagcg aggagagcag ggcccagttc gagaatattt tgtcgcgcga
    13861 gtcatgcaga atgtcggcgg acggaatggc gtccgacttt cttccgtttt ccacgcggtc
    13921 ggtgggcatg aggttccaga ggtcgttgca gtgaaagcgg gcccagggca gcatgtggtt
    13981 gacggccagc gtctgcgcct tgagcggcgt tccgctccag acgctccgaa gggagcctcg
    14041 gctgattgca gccttgtagg cgcttgcggt aaagtccgtg tcgcgggcgg actccggcgg
    14101 cagaaggcgc acgacgatgt cggcctgcgt aagggcgtgc tcagcgctgc acagggattc
    14161 aaagcgtgcg gagagtctgg cccacccgag aatcaagctg tcctcgagcc agggggcgat
    14221 gcggttgagt tcgagccaga gttcggcggg aaaggcgagg agaccgtagc gttcgatgat
    14281 tgatccgcgt cctgtaaaag gaggcctgcg accggcgtgc gtggcgacgt aggtgaaaac
    14341 ggggttcctg ccgtcatcct gaatgtaatg gatgggcccc ttgagagcgg tcgtgacgcc
    14401 atcgtcgaag agcagttgaa gcagtgaggt ctgatccttt gaacgtgcct cgtttcgcat
    14461 cagggattca aagacgccgt attggctatg acattttgtc atggtccgtt cgagcgcagt
    14521 gccgaagccg agctctctgt tctgatggat ctgtctgggc atgcgggggc cgctggcgag
    14581 ctgccagtag tccctgatcc atcgttcggc gatgaggccg agcggcacca gagctttgcc
    14641 attgtcgaag cggacgctgc gcggcatggt gcggttgatg tcgcagagtg cgcgtaggag
    14701 cgcgagcttg taggtcgagg ttttgacatc cttttcgaga atgtgcctga atcgtctgtt
    14761 gctcagctcg ctttgcgagt cgcgaagaaa aacgcaggac gctcatctgc actcgtgacc
    14821 gggagagccg acgccgtccc ggatttcctg cgaggccagc cgaaagccca tgcgttcgaa
    14881 gagcgtggca tagtcgagcg aatcgcgcaa gaaggtcttg cgatctggat cccccttgtg
    14941 gtcaagaggc acgacgacga tcagcgtgcc cttgtccgtg acagcacgct ccatgaagca
    15001 tgcgccttca tagagttcat ggtcggacag atgctgaaga actccgcagg tgtagacggc
    15061 gtcaaagcgg gggagacggg cgaatagtgc gtcttcggaa cccttgtcgg gaggaaggca
    15121 aagcagggag aaggagggcg actcggcgcc gaggagggcc tgagctctgg cctcggcgag
    15181 cttcagcagc ggcccgctcc cgtccgtggc ttcgacgatg gcgccgaggc ccgacatgaa
    15241 gcgggcatct cggctcgatc cgcaagcgag ctctagaacg cggcttccct tgggtatcca
    15301 tcgggtgagg agcgtttgaa tctcttgggg cgtttcaccc tcatagagcc tgaaaaaggc
    15361 ctcggaacgt gtttcgtaag cggacggcat cggcatggcg cactcctcag atctcgacga
    15421 ttcggcccgg attgagacgg ttgtcggggt cgaaggcgag tttcagggca cgcatggcgg
    15481 ccagctcctc cggtgtcttg gtgcgcttga gctcctgcgt cttgagactt ccgacgccgt
    15541 gctctgcggc gatggagccg ccttcggcaa ggactcggtc gtgcacgagc ctgtgaacgg
    15601 cttcttcatg cgggaaggcg acgccggcgg gaccgacgtt gaagtgcagg ttcccgtcgc
    15661 cgtagtggcc gaagacggag ggctttacgc ccgggaaggt ctcctcaagg gcggcgcttg
    15721 tttccctgat gaagcggacg aggcttgagc gcgggacgct gatgtcgtgc ttgacgtttc
    15781 cgccctcacg cttgacggcg gagggaatgg tctcacggca gcgccagaag gcctcgcggt
    15841 ccgattcgct gcggctgacg gcaccgtcga gaatcagtcc gttctcgaga agcggtgcca
    15901 gaacgccctc gagttcttct gaggattcgt cggaatcccg tcgccagtcg gagacgtcta
    15961 gaagcacctg ccagtcggaa agagagacgg ggggcgtcat gcccgtgacg gacgagagcg
    16021 attcgagcgg cgtgcgaccg atgagttcga aagcggtgag accggggccg aaggccgcct
    16081 caagtgtttc aaagagcgtt tcaacgtctt caaggcgatg aggcgtgatc caggccgaga
    16141 cgatggagcg gggcatgggc tcgagcgcga ggacggcttc ggtgatgacg ccgagcgtgc
    16201 cttcggatcc gatgaagacg tcccgcaggc tgtagcctga attgtccttt cgaagcccct
    16261 tcatgaggtc gagcacttcg cctgaggcga gcacgacctt gaggccgagc acctgacgcc
    16321 gcgccatgcc gtaccgcaac acgtgcacgc cgcccgcatt ggcggcgatg acgcctcctg
    16381 tctggcatga gccttcggcg gcgagcgaca ggggaagaag gcgccctgca tccgaggcgg
    16441 ccttctgtgc atcctgcaga acgacgccgg cctcaagcgt gatcgtgttg ttgacggtgt
    16501 cgacattcag gatgcgcttc atgtggccga gctgaaggat gagagttcgt cgcgctgtct
    16561 ctgcggaggc gccgggttca ggcgtggcgc ctccgacatt gctagtattg ccgccctgcg
    16621 ggatgacgga gagtccgtat gcggcggcag tcttcatgac gagagaaacc tcttcggtcg
    16681 aggcgggtcg cacgacggca agcgcttcgc cttgtctgcg tcctctgccg tccagagcgg
    16741 cggcggagat gtcggcggga tcggttatga gccccgtgtc gctcacggcg gagcggagtg
    16801 catcgatgat ttcttgatag gcaggcatgg cggaggacgg tttcattgga ggaatgcctc
    16861 aatcatacct tcggggaggg gatgaagagc ggggccctcc aaaaaggaaa agcccgaagc
    16921 gggtgcttcg ggcttgagcg ttgtcggcga aggtgcggaa ttacttcagc gcttcgaaga
    16981 cgcgggccgt gatggcgtca atggcaccga gaccggagat ggcgcggtac tgcggagcgg
    17041 tcttgtcacc ggacttggcg aggtcgctgt agaagccgac gagggcttcc gtctggctgt
    17101 ggtagacgtc gagacgcttg cgaacgactt cctcgcggtc gtcgtcacgc tggatgagcg
    17161 gatcacccgt cacgtcgtcc ttgccttcaa ccttcggcgg gttgtacttg atgtggtacg
    17221 tgcggccgga gacggggtcg acgcggcgac cggacatgcg ttcaacgatt tcggcgtcag
    17281 gcacctggat ttcgagaacg aagtcgatcg ggatgcctgc ggcctgaagg gcttcagcct
    17341 gcggaatcgt acgggggaag ccgtcgaaga gggcgccgtt cacggcgtcg ggctgcgtga
    17401 ggcggtcctt gacgagaccg atgatgatgt cgtcggagac gagctgaccc ttgtccatca
    17461 cggccttggc agccagaccg agcggggtgc ccgccttgac ggcggcacga agcatgtcgc
    17521 ccgtggagat ctggggaata ccgaactttt cgcagatgaa cttggcctgc gtgcccttgc
    17581 cggcaccggg aggcccaatc agaatcagac gcattggaat gctccttcga ttatgtaatg
    17641 aacgacgccg gagacttccg gcggcctgcc gtccggggga acggcggaga ataagcaaat
    17701 tttatcccga atccgcatga agtcaaagac cgtgcacgta ttcgcaacgg gaatcagacg
    17761 ggcgcgctct tttcgaagat cgcgcgcaca cggtcaagat ccttctgggt gtcgacgccc
    17821 gcaggaaggg cggcgtcaag caccatgacc gcaatgcgct cgccgtagta gagggcgcga
    17881 agctgctcca gactctcgag cttttccaaa ggagcctgag gcagggtggg aaagcggcgc
    17941 aggaagcctg cacggtaggc atagagaccg agatggtgaa gcggcgcgaa gccttcgggg
    18001 agactttcct ttgtttctcg gaagtggtct ctgggccagg gaatcggagc gcgggagaag
    18061 gtgagggcgg tgccgcggtc atcgagcacg accttgacga cgttgggatt gaagaagctc
    18121 tcgacgtcgt ggatggtgtg tgcggcagtc gccatggcgc agtcgggcct ggaggcgagc
    18181 aggtcggcca cgtctcggat gacgttaagc ggcatcagag gttcatcacc ctgaaggttg
    18241 acgacgatct cgtcatcggc aaggccgatc ttggcgacgg cttcggcaag tcggtcggtg
    18301 cccgtcgggt gatggggatc cgtcatgatc acgtcaaggc ctgcggctgc gcaggcctcg
    18361 tagacggacg catcgtccac cgccacgacg accctgtccg cgccgcagga gagggcgcgc
    18421 tcggcgactc ggacgatcat cggtctgccg cagatgtcgg cgagcggctt gtcgggaagg
    18481 cgcgaggact tgaggcgcgc aggaatgatc acggtaaagg acatgagaac ctcacttggg
    18541 atcgagtgcg gccgagcgct cggagtagtg gcgcgcctcg ttgtcgctca ggctgcgggc
    18601 ggcgttgcga agcatcacgg gaatgtcgtg gcggacttca aaggcgaggc ggcagcgcgg
    18661 gcagacgagc tcgcggcgct cgccgtcgcc ctgcagaagc ctgcccttgc agacaggaca
    18721 gacgatcatg tcgttgagtt tggcggacat tgttctcagt atccctttcg gaaaggatta
    18781 aaaccaacat tttaccgctc gtggtggggc ggaaaccata ataaaaacgc ctcgaaccgc
    18841 atcaggggat gcgggaggcg cttttcatgg cttgctgaag cgaggtcagt gcctgcctgc
    18901 ggcgtcgcgg gcgcgcttgt cgatcaggtc gatcaggtag gagtcgagct tgacttcaag
    18961 accgacgacc cagatgcgct tgtcgcctgc gagcgccgga tcgtggcggc acttgacggc
    19021 gtccttgccc gtgatgaaga tgatgtcctc ggtgcggttc gcaaagggat tctcggcgaa
    19081 gtcgaaatgg tcgccaagct cgagctcggc tccctcaatg tcatgggcgc ggatcatcgc
    19141 gaagaagcgt cccggagcgg cgatgccggc agcggagagc ggcttgagtt tttcggcgtg
    19201 aatcctgtcg gcaagctcgt cgatgagggc ggttctgccg gtcgcaagct gcgtgcaggc
    19261 gccgaagcaa ctcgaggcgg cgaagcgcgg cgtcctgctt tcgacgacgg cttcgttcga
    19321 tgtattgaga acgatggcgt ccacggtgtc gagacgggag ggaggctcgc gcaacgggcc
    19381 cgcgggaagc acccagccgt tgccgaggcc gcgcgcgccg acgaccgcga tttcgacgtc
    19441 gcgggcgaga gccgcatgct gcagaccgtc gtcgctcaca atcagatcca cttcgggatg
    19501 cgcctcgaga agcgctctgc ccgcttcaaa tcgggctcga ccgacggcaa cggggactcc
    19561 agtttccctt gcaatgagaa gaggctcgtc gccggcttcg gcggcaggag tctcctcgtt
    19621 gatcagtcga atgccgtctt cgcggcgtcc gaagccgcgg gagatgacgc ccgggcgcca
    19681 accgcgtgcc tgaagctcgc ggacaagggc gatcgtcacg ggcgtcttgc ccgtgccgcc
    19741 gacataaatg ttgccgacca cgacgacggg gacaggcaga cggttgggca cggtacgctc
    19801 gcggcgcgaa cgactgacgg cgaggaagac gagggagagc ggagaaagaa gccaggccac
    19861 ggggccgcgg gaggcccact ggcggtgagc ccaggcttca agaccggcgc gccatgtcat
    19921 gagagcacct ccgcatggat gtgcgaacaa acggacgctg cggcgtatgc cggcgggatg
    19981 gtcgaggaaa agggtgtagg gggtggcttg tcggacatga cgttgatgga agagcgcatg
    20041 cagttgcgcg ggagcggtaa tgataaacgg ttttcacccc ccggtgcgcg ccgcaagggc
    20101 gctttttgac gacagggacg gggctttcgg catcggaagt tatccacgat gttgtggata
    20161 actgtgtgga caagtcgctg gcttttccac gctcgctctc taccagagct tgcaaaagac
    20221 gggcgaagag ggcgaagact tctcccctcg tttgaaaaac atttgaaaat caagcgattg
    20281 cgtattttgt cagtgttcgg ggccttccta ctcagatgcg acttcgtgac tcgcctactg
    20341 ttgcagggat gtggacaacc ctgccgattt tctttctgtc cctattgact tttttatgaa
    20401 aatctgcatg
//
LOCUS       DBMDTI010000011        20170 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_891_length_20170_cov_3.552643, whole genome shotgun sequence.
ACCESSION   DBMDTI010000011 DBMDTI010000000
VERSION     DBMDTI010000011.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 20170)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20170)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20170
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_891_length_20170_cov_3.552643"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /gene="miaA"
                     /locus_tag="ACZPB6_01485"
     CDS             complement(<1..581)
                     /gene="miaA"
                     /locus_tag="ACZPB6_01485"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR1183182.1"
                     /translation="MDAIEKVLHECGQKPQALMILGPTAGGKSALSLEIARRHDVEII
                     SMDSALVYRGMDIGTAKPTKEERAVCPHHLIDIRDIGEAYSAADFLEDAVRLVGEIRS
                     RGRLPLIVGGTMLYAKALRDGINDMPSSTPEVREAVATEAAERGWPSMHEELGRIDPV
                     TAARLAPNDSQRIGRALEVWKMTGRPISAFHAE"
     gene            complement(581..2701)
                     /gene="mutL"
                     /locus_tag="ACZPB6_01490"
     CDS             complement(581..2701)
                     /gene="mutL"
                     /locus_tag="ACZPB6_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR1183183.1"
                     /translation="MPRTLAPKRSIAELPSQLISQIAAGEVIERPASVVKELVENAID
                     AGAGEIDVKLDGGGLKRIVVTDNGSGIPKDELALALRRHATSKIRDLLELERVGSLGF
                     RGEALASIDAVAEMSIRSRTPEADSAWEISDGEVHPAAGVVGTRVEVLDLFYKTPARR
                     KFMKSDATETAYVTEYLERLALANPDIGFTLSANGRNVLKLPAVTNVVDRIEALMPRA
                     FKGQHREVAAEEDGMTIRGLAGIPAIARSRADGQYFFVNGRYVRDKTLMHAVKAAYQD
                     VLHGQSQPVFCLYLTLDPTAVDVNVHPAKLEVRFRESSRVHQFVMHAVKAALAPPTAS
                     LLNGEETEVALPAQKRQEAARPVQSSAPSVFSSGSLGSIPAFTAPLRPVFKPESCPDV
                     STAMRLFGADVDTARAAAQGRVPEGRFSGRKDGNWLKSEKVPPAEPESGRLDLQGGSF
                     LKETNEATSLGPIDVPPPETAEGMSFEEIAEAVPSASDKTSACVAATPASEACKPATL
                     PPGTLGRAIAQIGGIYILAENERGLVVVDMHAAAERITYERLKREMDARGIAVQQVLI
                     PQVFRVNPLLFSVFEENRELLSTLGLELTGAGDCAVAVRALPMLCKDMPSQTVETMVK
                     CLLEDLQNFGETSVIEELRNRLLATVACHGSVRAHRRLTLPEMDRLLRDMEETERADQ
                     CNHGRPTWRQMTLTDLDKLFMRGK"
     gene            2852..3523
                     /locus_tag="ACZPB6_01495"
     CDS             2852..3523
                     /locus_tag="ACZPB6_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR1183184.1"
                     /translation="MDILDLVVNLFTNTDAFLVSLATDYGTLIYAAMFLIFFLETGFV
                     IMSFLPGDSLLFVAGTVAASGTASPWLIMLAVIVGAIAGNTLGYEQGRWLGNRIYSGN
                     ISWINEKKLRHAHDFYMRHGGKTILLARFVPIVRAFAPLIAGAARMNGIRFELFSGTG
                     AILWAVSIVGAGYLFGNIPFIKNNLSLILLLGIVAAISGPVLVGMVWKFIQKRKGLDA
                     EDPQK"
     gene            complement(3581..4123)
                     /gene="rfbC"
                     /locus_tag="ACZPB6_01500"
     CDS             complement(3581..4123)
                     /gene="rfbC"
                     /locus_tag="ACZPB6_01500"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR1183185.1"
                     /translation="MTTQLHVTALGLPGVLLLKSIVHADVRGSFTQSWQAADWRAAGV
                     DIAFMQDNLVCNARRGTLRGMHWQREPHAQAKAVQCVRGALLDVVADVRPESSLFGRW
                     ISVELKAGEGESLYVPAGYAHGYVTTEDDTIVLYKVDKPWVPGSEASCRWNDPTLNID
                     WRCENPILSEKDAAAPFLLP"
     gene            complement(4219..5463)
                     /gene="pepT"
                     /locus_tag="ACZPB6_01505"
     CDS             complement(4219..5463)
                     /gene="pepT"
                     /locus_tag="ACZPB6_01505"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIR1183186.1"
                     /translation="MKEAILERFLRYVAVTSQSDGKAGVVPSTEGQRKLAGMLAEELA
                     ELGFVNIEISEYSVLTALLPGNLPDNKAVPTVGWCCHLDTVDAGLSPDVHPQVIRGYQ
                     GGDICLNKEKDLWMRTSEHPEIEKYVGDDIVVTDGTSVLGADNKSAIANVMTAFDIVV
                     KENRPHGDIYVAFVPDEEIGLLGAKKIDFSKFPVDFAYTVDCCELGEVVYETFNAGSA
                     TIRIKGVTAHPMSSKGVLVNPTLIATDFVQMMDRGSTPECTEGTEGFVWVNSIVSNPS
                     QATVSIKIRDHNREKYEAKKALIASAVEYLKVRNPRARIELEVKDMYGNIADALTDDN
                     RCAVDHLYRALEIAGVKANTIAMRGGTDGSFISTQGVITPNYFTGGHNFHSNCEFLPL
                     GAAVKSTEVTLTLIDLIAGTKH"
     gene            complement(5589..6791)
                     /locus_tag="ACZPB6_01510"
     CDS             complement(5589..6791)
                     /locus_tag="ACZPB6_01510"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR1183187.1"
                     /translation="MHVLTLEKLAKDGLLAGKRVLIRSDLNAPHDENGRITNETRLVA
                     SVPAIRTALDAGAAVMVMSHLGRPTEGTVTPADSLAGVARRMGELLGCDMPLVADWVD
                     GVKVGPGHVVMLENCRCNVGEKKCNDELSKKYAALCDVYVNDAFGTAHRAQASTVGVA
                     RYADVACAGPLMAAEIDALTKSVEEARHPLAAIVGGSKVSTKLTILSNLAEKVDRLIV
                     GGGILNTFLLAAGKPIGKSLAEPELVDECRKVLAVLAAKGASLPMPVDVVVAKAFSAD
                     AEHRVADVDDVAEDEMILDVGPKTAAILADILKSAGTIVWNGPVGVFEMAPYAEGTRV
                     MADAIAEATDKGAFSIAGGGDTVSAVHQFGIADRVSYISTGGGAFLEFLEGKKLPAVA
                     VLEERAAQ"
     gene            complement(6910..7926)
                     /gene="gap"
                     /locus_tag="ACZPB6_01515"
     CDS             complement(6910..7926)
                     /gene="gap"
                     /locus_tag="ACZPB6_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR1183188.1"
                     /translation="MTIRVAITGFGRIGRNVLRAHYEGGKKHDVEIVAINAPGDLAIH
                     AHLLKHDSCHGRFNADVQVEGTDTLIVNGDRIRVFATRDPKEIPWGELNVDVVMECTG
                     AFNSKEKSMVHIEQGAKKVLLSAPGKTADATVVYGVNQEVLKASDLVVSNASCTTNCL
                     APLAKALHRTVGIERGLMTTVHSYTNDQVLTDVYHKDMRRARAATLSMIPTKTGAAKA
                     VGLVLPELNGLLDGFAVRVPTINVSFVDLTFIAKRDTSVEEINALVKAASESEDLAGI
                     LGYNDELLVSTDFNGDDHSSIFDSTLTKVSGNRLVKVTAWYDNEWGFSNRMLDTACAM
                     MAAK"
     gene            complement(7950..9962)
                     /gene="tkt"
                     /locus_tag="ACZPB6_01520"
     CDS             complement(7950..9962)
                     /gene="tkt"
                     /locus_tag="ACZPB6_01520"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1183189.1"
                     /translation="MTSQASAQMMANAIRALSMDAVQKAKSGHPGMPMGMADIATALW
                     SRHLRFNPTNPKWTGRDRFILSNGHGSMLQYALLHLTGYDLSIDDLKAFRQLHSKTPG
                     HPEVDVTPGVETTTGPLGQGIANGVGMALAEKMLANEFNRGGFPVFDNRTYVFLGDGC
                     MMEGISHEVCSLAGVWKLNKLIAIYDDNGISIDGDVKGWFGDDTRGRFEAYGWNVIGP
                     VDGHDIDAMDAAIAEAKLSEDKPTLIIARTTIGKGSPNRQGTAKVHGEALGDEEIAAT
                     RAAIGWPYAPFEVPQEVYDAWDHRAEGARIEAEWQTMFDGYAAQYPELAAELKRRLAG
                     DLPENWSDTVMDALCAAEEAAETVATRKASQKALNALAPALPELLGGSADLTGSNLTN
                     WTGVKSLNSGDFLARHISYGVREFGMSAIMNGIALYGGFIPYAATFLTFSDYSRNALR
                     MSSLMKQRVINVFTHDSIGLGEDGPTHQSVEHVPSLRLIPGMHVWRPADTVETIIAWA
                     SAIERRNGPSCLVFTRQNVPFLDRDEVDADAIARGAYVAAEAPAGAGEAEVVLLATGS
                     EVGLAMKARAELTARNVQVRVVSMPCANLFDEQDEEYRRAVLPEGVPVLAIEASKSDL
                     WYKYFSGKGAVIGVDTFGESAPACVLWERFGFTVENVVAKVEQLLK"
     gene            10263..11510
                     /locus_tag="ACZPB6_01525"
     CDS             10263..11510
                     /locus_tag="ACZPB6_01525"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HIR1183190.1"
                     /translation="MSIRQGFSFKLKPNGAQDKKMSRFAGCTRWVYNQGLAWNEERRT
                     ADPAFHLSYAKLCTELLVWKEQNPWLRETHSQVLQQSLKDLMDGFQKFFKGLTAFPKK
                     HKKFVTTDSFRFPQGFKIDEGRRQIYLPSIGWVKYKRSRFIQGKAKNVTVSRQTDGWY
                     VSIQTEYEMEPPKHAGDSIGIDMGCVRFCTLSDGTFFEPCGALKKALKKLARMQRRLA
                     RMQKFEKNWRKQKAAIARLHQHIANVRRDFIEKVSLNICKNHAEIFREDLKIKNMTAS
                     AKGTVEEHGTRVAQKSGLNRSILDQGWRIFFSKLDWKALRLGGHVHTVPPQNTSRTCP
                     ICGCVSKDNRKTQALFKCVSCGHQGNADVVAAINIKERGRSSLACGESERNAQAQVNL
                     GRSKKPAPNQQQESTEVISSASC"
     gene            11563..12315
                     /locus_tag="ACZPB6_01530"
     CDS             11563..12315
                     /locus_tag="ACZPB6_01530"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR1183191.1"
                     /translation="MAAPRFYVEVETEALDKALAEQAEVVLPPKAAHHAGRALRLRTG
                     EETVVFNGTGRQWRGRIRFDQDGAYVALDAVETPEVEPPVRMTLVQALVSPEKLDWIV
                     EKAVETGVSEIVLTPAARSVTKLAGERLEKRLQKLRDIAVSAAEQCGRNVVPEIRGAS
                     SFKEAMLGTQADRRLVLAPGAAHGTRLTGEEKSVAFAVGPEGGFSPEEIDLAEEAGWL
                     AVLLGPRVLRTETAGIAAAVWLNTLTGDYGER"
     gene            complement(12319..13227)
                     /locus_tag="ACZPB6_01535"
     CDS             complement(12319..13227)
                     /locus_tag="ACZPB6_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013618.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR1183192.1"
                     /translation="MTFRKQPRQFRISHLVSVSVLALTLGGAAFWSDSDYASTAPALS
                     YAQEDLNARADLRQREGVLSGVTPSLSLLDAPAAAREEATASSEELTVEDNVIHEGSP
                     RHLVAEYISRTFRVSMRDARQITDWAVEIGEARDLDPLLILAVIGTESSFKPTARSNA
                     GAEGLMQVMTSVHEAKFDAFGGPEAAFDPYANMVVGTDILSYLIRRTGSVRRALKWYS
                     GAANLEDDRGYGARVMREHGLLTVAAEGRTDAAVKLHRAGKSASEGGAGNAAKLGFAY
                     WTKLSERTEGAPRARNADLRGKAQAS"
     gene            13511..13795
                     /locus_tag="ACZPB6_01540"
     CDS             13511..13795
                     /locus_tag="ACZPB6_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HIR1183193.1"
                     /translation="MKRRPIHRGGILSAGYDARARHLDVEFDTHRLVRVENIGPEAAD
                     RFLNSGSPGSYWRDVIEEGYTIREISKAEGDAPAAGKKGMDDLKRLFGDL"
     gene            complement(13830..15320)
                     /gene="mgtE"
                     /locus_tag="ACZPB6_01545"
     CDS             complement(13830..15320)
                     /gene="mgtE"
                     /locus_tag="ACZPB6_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR1183194.1"
                     /translation="MPHSLKDYDELHDGQGHLVRDSEEASRALEWVQQILEKDQDEHH
                     GGVERKGDEDDEALPISDEAATELRQLLEPLHPADVAWILEALPLDERLAVWNLVKSE
                     SDGDILVEVNDGVRETLIDAMDRDELVDAVETLDTDEIADLVDDLPPDVMAQVQEGLS
                     HEERAQLRAAMSYPEESVGARMDFEIVSIREETTIELVLRYLRRFETLPDHTDQVFVV
                     DRLGHLKGTLSLSQILTTDPAVPVKDLMHTDMLTLNPLEDSSDAAQAFERYDLVSAPV
                     VDEAGRLVGRLTVNEIVDVIREESEEDAFAAVGLDDEQDIFASVWEAARSRWLWLGVN
                     LCTAFFASRVISAFDGTIERVVALAALMPIVAGMAGNSGNQTLTLLVRSLAMGQVTEA
                     NKADLIKKEVLVSIINGILWGAIAGLCAWGLYWDAPEGRMLGLVMFLAMLLNIIQGAV
                     VGLAVPLILRYFGRDPAMGGSVLLTFTTDSGGFLIFLGLATFFFAS"
     gene            complement(15491..17485)
                     /locus_tag="ACZPB6_01550"
     CDS             complement(15491..17485)
                     /locus_tag="ACZPB6_01550"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HIR1183195.1"
                     /translation="MNLFFEEDGSFKAGTVLSQAGNAYQVQLPTGRRTKIKASHSFFE
                     FEQPAPADLVTRAQALVPELDPAFLWEVAPEGEFTYADLAADYWGGSAGPVEKAALLW
                     ALHGNPVYFYRKGRGGYRRAPEDILQKALEALEKKRRLEEQRKAWTAEMVEGTLPDVI
                     RRNAMTLLIRPDKNGIEWKALNDASAETRQTPLRLMLSLGGIASPWRWHVDSFYAMNF
                     PQGRGFPSDLPLPPEADWSQYPLADVRAFSIDDSDTTEVDDAASVVHLEGGRTRVGIH
                     IAAPALGILRDDPLDKVARARMSTVYAPGLKTTMLPDPWIKAFSLDEGRAVPCLSLYV
                     TVDDETFSVEKTETRLERVSVTRNLRYDKIDSLVTEEAIQSGTLDVEFADEICWLWRF
                     AKKLQKDREEVRGRPEPVGRVDWFFALEGEGEDALIRVKGRRRGAPLDLLVAELMIFA
                     NSTWGLWMEEHGTPGIYRSQRMGRVRMSTTPGPHDGLGVVRYAWSTSPLRRYVDLVNQ
                     RQMITAVLGEPPAYQGNDVELFTIVSQFENIYTLYGDFQTRMERYWSLRWIMQESVGQ
                     IEAIVVKGDLVRIDGLPFMQRVPGLPEDLPRGRKVLLQIMGCDLVDLVMDSRLLRVLD
                     ETADTSDEDEDEEAALEEQAAVEGGEEAQGGDTVSESSAS"
     gene            complement(17581..18159)
                     /locus_tag="ACZPB6_01555"
     CDS             complement(17581..18159)
                     /locus_tag="ACZPB6_01555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017538.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HIR1183196.1"
                     /translation="MTSILYLHGFLSSPESNKVRVLRAAAPDGVRVFAPDLNVPPLEA
                     DRIISGLVGQAEADGLTVVGSSLGGFWAARAAVRHGLRCVLLNPCFNPWVFVGRHVGV
                     QTIYGTDRRIEVKASAADELMALAEEVSPLAVDPGRTLVVLGTADETLDWQAGARAYA
                     ACRQVIIPGGDHRISNIGAVMPRIMDFILNEG"
     gene            complement(18174..19535)
                     /gene="mpl"
                     /locus_tag="ACZPB6_01560"
     CDS             complement(18174..19535)
                     /gene="mpl"
                     /locus_tag="ACZPB6_01560"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIR1183197.1"
                     /translation="MHIHILGICGTFMGGVAQLACEAGHKVTGCDAHVYPPMSDALRN
                     AGIEIMEGYDVSQLDAVRPDVFVIGNAISRGNPLLEAILNRGLSYTSGPAWLCEHVLA
                     GRHVLAVAGTHGKTTTTSMLAWILKEAGLEPGFLIGGVPQWSPRSASLGRVAPMSPFV
                     IEADEYDTAFFDKRSKFVHYRPRTAILNNLEYDHADIFENLAAIEKQFHHLVRIMPSE
                     GLVVANGRCEALDRVLAKGCWSALERFDSQDGWSLSDDGEAAFEGRSFGRLQLGMPGR
                     YNELNALAAVAAARSVAVPPEKSLEALAAFAGVKRRMEVRGVEDGVTVIDDFAHHPTA
                     IAETIHAVRSRMTSGRLLAVLEPRSNTMKLGTMKDRLAGSLHEADEVFCFAGKGVAWE
                     PAEVLDVMGERAFCTHDLDVLVEAVLKEARPGDTVLCMSNGAFGGIHGKLLAGLACRR
                     EAQ"
     gene            19673..>20170
                     /locus_tag="ACZPB6_01565"
     CDS             19673..>20170
                     /locus_tag="ACZPB6_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183198.1"
                     /translation="MQPNDLPRSKESSAAAQAAVLIASHRMSRDRAVETVVRSTGVRP
                     TDAAVASELRRWFACFDPEGHKALLDKKRLAALSVLSSLSDWSPLLTGSVLAGDAVED
                     DPVRILIRSDDEKAVELALLAMDVEYEPVESSERGRSFRTVLQTECRGEDVLITVTNS
                     HRAAKP"
BASE COUNT         3772 a         6265 c         6396 g         3737 t
ORIGIN      
        1 ctctctgcat ggaaggccga gatcggcctg cccgtcattt tccagacctc cagagcgcgc
       61 ccgatgcgct ggctgtcgtt gggcgccagt ctggcggccg tcacgggatc gatcctcccg
      121 agttcttcat gcatggatgg ccagccccgt tcagccgcct cagtcgccac cgcctcgcga
      181 acctcaggcg tgcttgacgg catgtcgttg atgccgtctc tgagagcctt ggcgtagagc
      241 atcgtgccgc ccactatgag cggaagccgc cctctcgagc ggatctcacc cacgagtctc
      301 acggcatcct ccagaaaatc ggcggcgctg taggcctcac caatgtcacg gatatcgatc
      361 agatggtgcg ggcataccgc cctttcctcc ttcgtcggct ttgccgtgcc gatgtccatg
      421 cccctgtaga caagcgcaga atccatggag atgatctcaa cgtcatgacg tcgggcgatc
      481 tccagagaaa gagcggactt gccgcccgcc gtaggcccaa gaatcatcag cgcctgcggc
      541 ttttgcccgc attcatgcag gaccttttca atcgcgtcca tcacttgccc ctcatgaaga
      601 gcttgtccaa gtctgtaagc gtcatctgcc gccatgtcgg acggccgtgg ttgcactggt
      661 cggcccgctc ggtctcctcc atgtccctca gaagcctgtc catttcgggc agggtgagac
      721 ggcggtgcgc gcgcacggag ccatgacagg cgaccgtggc gagaagcctg tttctgagct
      781 cctcaatgac agacgtctca ccgaagttct gaagatcctc tagaagacac ttcaccatgg
      841 tctccaccgt ctgggacggc atgtccttgc agagcatcgg gagcgcacgc acggcgacgg
      901 cgcagtctcc ggcccccgtg agctcaaggc cgagcgtgga aagaagctcg cggttttcct
      961 caaagacgga aaagagaagc ggattcacgc ggaagacctg cgggatcaga acctgctgta
     1021 cggcgatgcc gcgggcgtcc atctcgcgct tgagacgctc gtaggtgatg cgctcagccg
     1081 ccgcgtgcat gtcaaccacc acgagcccgc gctcattctc cgccagaatg tagatgccgc
     1141 cgatctgggc aatggcgcgg ccgagcgtcc ccggaggaag cgtcgcgggc ttgcaagctt
     1201 cagatgcggg agtggccgca acacatgccg aggtcttgtc ggaagcggac ggaaccgcct
     1261 cggcaatctc ctcgaagctc atgccctcgg ccgtttcggg cgggggcacg tcaatcggcc
     1321 cgagagacgt tgcctcattc gtctccttca ggaaggatcc tccctgaagg tcgagacgtc
     1381 cggattcggg ttcggcaggc ggcactttct cgctcttgag ccaattgccg tctttccgtc
     1441 ccgagaagcg tccctcagga actctgccct gcgcagccgc tctggcagta tcgacgtccg
     1501 cgccgaaaag gcgcatagcc gtgctgacgt caggacagga ttcgggcttg aacacgggac
     1561 gcaaaggtgc ggtgaatgcg ggaatgctac cgagcgagcc tgatgaaaaa acggacggtg
     1621 cagacgactg cacgggacgt gcggcctcct gccgtttctg agcaggcagg gctacctcgg
     1681 tctcctcacc attcagaagc gaggcggtgg gcggcgcgag agccgccttc acggcatgca
     1741 tgacgaactg gtgtaccctg ctcgactccc tgaacctgac ctcaagcttg gcgggatgta
     1801 cgtttacgtc gaccgcagtc ggatccagcg tcagatagag acagaagacg ggctggctct
     1861 ggccgtgcag cacgtcctga taggcggcct tgacggcatg catgagcgtc ttgtcgcgca
     1921 catagcggcc attgacgaaa aagtactgcc cgtccgcacg actgcgagcg atggccggaa
     1981 tgccggcgag tcctcgaatc gtcatgccgt cctcctcggc cgccacctcg cggtgctgtc
     2041 ccttgaaggc tctgggcatc agcgcctcaa tgcggtcaac aacgttcgtg acggcaggga
     2101 gcttcagaac attgcggcca tttgccgaaa gcgtgaagcc gatgtcgggg ttggcgagcg
     2161 ccaggcgctc gagatattcg gtcacataag ccgtttccgt cgcgtcggac ttcatgaact
     2221 tgcggcgagc cggcgtcttg tagaaaaggt cgagcacctc cacgcgcgtg ccgacgacgc
     2281 ctgcggcggg atgcacctct ccgtcggaga tctcccatgc gctgtcggcc tcaggcgtgc
     2341 ggctgcggat gctcatctcg gcaacggcat cgatcgacgc cagggcctcg ccgcgaaagc
     2401 cgaggcttcc gacgcgctcg agctcaagaa ggtcgcggat cttgctggtg gcgtgccgtc
     2461 tgagggctag cgccagctcg tccttcggaa tgcctgagcc gttgtcggtt acgacgatgc
     2521 gcttgaggcc accgccatcg agcttgacgt cgatctcacc ggcgccagcg tcgattgcat
     2581 tctccaccag ctccttcaca acggaggcag gacgctcgat cacttcgcct gccgcaatct
     2641 ggctgatgag ctggctcggt agttcagcaa tgcttcgttt gggcgcaagc gtgcgcggca
     2701 tcaggttttg cataagcctc gggcaatttc aattcgttaa gatatgcccc gtcagccggg
     2761 gactgcattc agtatgatat gtgcaatctc cgctccttgc acatggccgg ccctccctcg
     2821 ggggcaggaa acaatcacct gggattctca gatggacatc ctcgatctcg tagtcaacct
     2881 ctttaccaat acggacgcct ttctcgtttc cctcgccacg gactacggca ccctcatcta
     2941 cgccgccatg ttcctcatct tcttcctgga gactggcttc gtgatcatgt cattcctgcc
     3001 gggagactct cttctcttcg ttgcaggcac cgtcgccgcc tccggtacgg ccagtccctg
     3061 gctcatcatg ctcgccgtca tcgtcggcgc catcgcaggc aacacgctcg gctacgagca
     3121 gggccgctgg ctcggcaacc gcatctacag cggcaacatc tcctggatca acgaaaagaa
     3181 gctccgccac gctcacgact tctacatgcg ccacggcggc aagaccatcc tgctcgcccg
     3241 cttcgtgccg atcgtccgcg ccttcgcgcc gctcattgca ggcgcagccc gcatgaacgg
     3301 catccgcttc gagctcttca gcggcacagg tgccattctc tgggctgtct cgatcgtcgg
     3361 cgcaggctac ctcttcggca acatcccgtt catcaagaac aacctctctc tcatccttct
     3421 cctcggcatc gtcgccgcca tctcaggccc cgtcctcgtg ggcatggtct ggaagttcat
     3481 ccagaagcga aagggacttg acgcggaaga tccgcaaaaa taagtcgcct tgtgagaaaa
     3541 gcctgccgga tcggcaggct tttctgcatt cagacctttc tcacggcaaa aggaaggggg
     3601 ctgcggcgtc cttctcggag aggatgggat tttcgcaccg ccagtctatg ttcagcgtcg
     3661 gatcattcca tcgacacgag gcctccgacc ccggcaccca tggcttgtcg accttgtaga
     3721 gcacgatcgt atcgtcctcc gtcgtcacgt agccgtgcgc atagccggct ggcacataga
     3781 gagactcccc ctcgccagcc ttgagctcga cggaaatcca cctgccgaac agcgagctct
     3841 cgggcctcac gtctgccaca acgtcaagga gcgctcctct gacgcactgc acggccttgg
     3901 cctgcgcatg cggctcacgc tgccagtgca tgccgcgcag cgtccctctg cgggcgttgc
     3961 agacgagatt gtcctgcatg aaggcaatgt ccacgcccgc cgcgcgccag tcagccgcct
     4021 gccagctctg cgtgaagctg ccgcgcacat ccgcgtgaac gatgctcttc aaaagcagga
     4081 cgcccggcag accgagtgcc gtcacatgaa gctgtgtcgt catgatttcc ctttccgttt
     4141 ccgtcctgcc gccctcacga aaacgggcgt ccactcacag cgtgaggacg cccgttcagg
     4201 ttcagagcaa gcctgacctt agtgcttcgt gcctgcaatc aggtcgatca gggtgagcgt
     4261 cacttccgtg ctcttcacgg ccgcaccaag cggcaggaat tcgcagttgc tgtgaaagtt
     4321 gtggccgccc gtgaagtagt tgggcgtgat gacgccctgc gtcgagatga aggaaccgtc
     4381 cgtgccgccg cgcatggcga tcgtgttggc cttgacgccg gcgatctcga gcgcgcgata
     4441 gagatggtcg acggcgcaac ggttgtcgtc cgtgagcgcg tcggcgatgt tgccgtacat
     4501 gtccttcact tcaagttcga tcctggcgcg cggattgcgg accttcaggt attcgacagc
     4561 ggaggcgatg agcgccttct tggcctcgta cttctcacga ttgtggtcgc ggatcttgat
     4621 cgaaaccgta gcctgcgagg ggttcgagac gatgctattg acccaaacga agccttcggt
     4681 gccctcggtg cattcaggcg tcgaaccacg gtccatcatc tgcacgaagt ccgtggcgat
     4741 gagagtcggg ttgacgagaa cgcccttgga ggacatcgga tgggccgtga cgcccttgat
     4801 gcggatcgtg gcgctgcctg cgttgaaggt ttcgtagacc acttcgccga gctcgcagca
     4861 gtcgaccgtg taggcgaagt cgacggggaa cttcgagaag tcgatcttct tggcgccgag
     4921 aaggccgatc tcctcgtcgg ggacgaaggc gacgtagatg tcgccgtgcg ggcggttttc
     4981 cttcacgacg atgtcgaagg ccgtcatgac gttggcgatg gccgacttgt tgtccgcacc
     5041 gagcaccgag gtgccgtcgg tgacgacgat gtcgtcgccc acgtacttct cgatttcagg
     5101 atgctcggac gtgcgcatcc acaggtcctt ttctttgttc aggcagatgt cgccaccctg
     5161 atagccgcgg atcacctgag gatggacgtc ggggctgagg ccggcatcca cagtgtcgag
     5221 atggcagcac caaccgaccg tcggaacggc cttgttgtcg ggaagattgc ccggaagcag
     5281 tgccgtcagc acgctgtatt cggaaatctc aatgttgacg aagccgagtt cggcaagctc
     5341 ttcggcgagc atgccggcga gcttgcgctg gccttcggtg ctcgggacga cgcctgcctt
     5401 gccgtcgctc tggctcgtga cggccacata gcgcaggaat cgttcaagaa tagcttcctt
     5461 catgatgttt tctctcctca aaggggtttc tctgcatgcg gttggcgtcc cgcctcaggc
     5521 ggggcgcctt gttgtccgga cggaggccat gggtcctgcc gccgtccgga tgcggatcag
     5581 cgatcctgtt actgagcggc gcgctcttca agcacggcca cggccggaag cttcttgcct
     5641 tccaggaact caaggaaggc gccgccgccc gtggagatgt aggagacgcg gtcggcaatg
     5701 ccgaactggt gcacggcgga aaccgtgtcg ccgccgccgg cgatcgagaa ggcgcccttg
     5761 tcggtcgcct cggcgatggc gtcggccatc acgcgggtgc cttcggcgta gggagccatt
     5821 tcaaagacgc cgaccgggcc gttccagacg atcgtgccgg cgctcttgag aatgtcggca
     5881 aggatcgcgg cggtcttggg accgacgtca aggatcattt catcctcggc aacgtcgtcc
     5941 acgtcggcaa cgcgatgctc ggcgtcggcc gagaaggcct tggcgaccac gacgtcgacg
     6001 ggcatcggca ggctggcgcc cttggcggcg agcacggcga gcaccttgcg gcattcatcg
     6061 acgagctcag gctcggcgag ggacttgccg atcggcttac ccgcagcgag aaggaacgtg
     6121 ttgagaatgc cgccgccgac gatcaggcgg tcgacctttt cggcgaggtt ggagaggatc
     6181 gtgagcttcg tggagacctt ggagccgccg acaatggcgg cgagcgggtg acgggcctcc
     6241 tcgacggact tcgtgagggc gtcgatttca gcagccatca gggggccggc gcaggcgacg
     6301 tcagcgtagc gggcgacgcc gaccgtggaa gcctgggcgc ggtgggccgt gccgaaggcg
     6361 tcgttgacat agacgtcgca gagggcggcg tacttcttcg agagttcgtc gttgcacttc
     6421 ttttcgccaa cgttgcagcg gcagttttcg agcatcacga catggccggg cccgaccttg
     6481 acgccatcaa cccagtcggc gacgagcggc atgtcgcaac ccagaagctc gcccatgcgg
     6541 cgggcaacgc ccgcgaggct gtcggccggc gtcacggtgc cttccgtcgg acggccgagg
     6601 tggctcatca ccatcacggc ggcaccggcg tcaagcgcgg tgcggatggc gggaacgctg
     6661 gccacaagac gggtttcgtt ggtaatgcgg ccgttttcat cgtgcggcgc gttgaggtcg
     6721 ctgcggatca ggacgcgctt gccggcgaga agaccgtcct tggcaagctt ttcaagagtc
     6781 agaacgtgca tgacttgttc tccgaaaggg agtgcggcgc attgcgccgc tcaggaaaca
     6841 taaagtgcga aagccacgcc gaggatgcgg atccggggca tggctttcat gatcatcgga
     6901 acttgccgtt tacttggcgg ccatcatcgc gcaggccgtg tcaagcatgc ggttggagaa
     6961 gccccattcg ttgtcgtacc aggcagtgac cttcacaaga cgattgccgg agaccttcgt
     7021 cagcgtggag tcgaagatcg agctgtggtc atcgccgttg aagtcggtgg agacgagaag
     7081 ctcgtcgttg tagccgagga tgccggcgag gtcttcggac tcggaggcgg ccttcacgag
     7141 ggcgttgatt tcctcgacgc tcgtgtcgcg cttggcgatg aaggtgaggt cgacgaagct
     7201 gacgttgatc gtcgggacgc ggacggcgaa gccgtcgagc aggccgttga gctcaggaag
     7261 cacgaggccc acggccttgg ctgcgccggt cttcgtcggg atcatggaaa gcgtggcagc
     7321 gcgagcgcgg cgcatgtcct tgtgatagac gtcggtgagg acctggtcgt tcgtgtagga
     7381 gtggaccgtg gtcatcaggc cacgttcgat acctaccgtg cggtgcaggg ccttggcgag
     7441 cggagcgagg cagttcgtcg tgcaggaggc gttggagacg acgaggtcgg aggccttgag
     7501 gacctcctga ttgacgccgt agacaaccgt agcgtcagcc gtcttgccgg gagcggagag
     7561 caggaccttc ttggcgccct gctcgatgtg caccatgctc ttttccttcg aattgaaggc
     7621 gcccgtgcac tccatcacga cgtcgacgtt gagttctccc caggggatct ccttcgggtc
     7681 gcgcgtggcg aagacgcgga tgcggtcgcc gttgacgatc agcgtgtccg tgccttcgac
     7741 ctgcacgtca gcgttgaagc ggccgtggca ggagtcgtgc ttgagaagat gggcgtggat
     7801 ggcgaggtcg ccgggggcat tgatcgcgac gatttcgacg tcatgcttct tgccgccctc
     7861 gtagtgagcg cggagaacgt tgcggccgat gcggccgaaa ccggtgatgg caacacgaat
     7921 ggtcatgtct aatccttctg tctttgttgt tacttgagaa gctgctcaac cttggcaacc
     7981 acgttttcaa ccgtaaagcc gaaacgttcc cagaggacgc aggccggcgc gctttcgccg
     8041 aaggtatcga cgccgatcac ggcacccttg ccggagaagt acttgtacca gaggtcggac
     8101 ttggaagcct cgatggcaag cacgggcacg ccttcgggca gcacggcgcg gcggtattcc
     8161 tcgtcctgtt cgtcgaagag gttcgcacag ggcatggaaa cgacgcggac ctgaacgttg
     8221 cgggccgtca gctcggcgcg ggccttcatg gcgagaccaa cttcggaacc cgtggcgaga
     8281 agcacgactt cggcttcacc ggcgccggca ggcgcttcgg cggcaacata agcgccgcgg
     8341 gcgatcgcgt cggcatccac ttcgtcacga tcgaggaacg gaacgttctg gcgcgtgaac
     8401 acgaggcagg acgggccgtt gcggcgctcg atggcgctcg cccaggcgat gatcgtctcg
     8461 accgtgtcgg cgggacgcca gacatgcatg ccggggatga ggcgcagact gggcacgtgc
     8521 tcgacggact gatgcgtcgg gccgtcttca ccaaggccga tcgagtcatg cgtgaagacg
     8581 ttgatgacgc gctgcttcat gagggaggac atgcgcaggg cgttgcggga atagtcggag
     8641 aacgtcagga acgtggcggc gtacggaatg aaaccgccgt agagcgcaat gccgttcatg
     8701 atggcggaca tgccgaactc acggacgccg tagctgatgt ggcgggcaag gaagtcgccg
     8761 ctgttaagcg acttgacgcc ggtccagttc gtgaggttcg agcccgtcag gtcggcggaa
     8821 ccgccgagca gttcgggaag cgcgggcgca agagcgttga gggccttctg gctggccttg
     8881 cgggtggcga cggtctcggc agcctcttcg gcggcgcaga gggcgtccat cacggtgtcg
     8941 gaccagtttt cgggcaggtc gccggcgagg cggcgcttga gttctgcggc gagctcggga
     9001 tactgggcgg catagccgtc gaacatcgtc tgccattcgg cttcgatgcg ggcgccttcg
     9061 gcacggtgat cccaggcgtc atagacctcc tgcggaacct cgaacggagc gtagggccaa
     9121 ccgatcgcgg cgcgggtggc ggcgatttcc tcatcgccga gcgcttcacc gtggaccttg
     9181 gcggtaccct gacggttggg cgagcccttg ccgatggtgg tgcgggcgat gatgagggtg
     9241 ggcttatcct cggagagctt ggcctcggcg atggcggcgt ccatggcgtc gatgtcatgg
     9301 ccgtcgaccg ggccgatcac gttccagccg taggcttcga agcggccgcg cgtgtcgtcg
     9361 ccgaaccagc ccttgacgtc accgtcgatc gaaatgccgt tgtcgtcgta gatggcgatg
     9421 agcttgttga gcttccagac gccggcgagc gagcagactt cgtggctgat gccctccatc
     9481 atgcagccgt cgcctaggaa gacgtaggtg cggttgtcaa agacagggaa acccccgcgg
     9541 ttgaattcgt tcgcaagcat cttttcggca agcgccatgc cgacgccgtt ggcgatgccc
     9601 tgaccgagcg ggccagtcgt cgtttcaacg ccgggcgtga catccacttc gggatggccg
     9661 ggcgtcttgc tgtgcagctg acggaaagcc ttcagatcgt cgatcgaaag gtcgtagccg
     9721 gtgaggtgca gcagagcgta ctgcagcatc gagccatggc cgttcgaaag gatgaagcgg
     9781 tcgcggccgg tccacttcgg attcgtcgga ttgaaacgaa ggtgacgcga ccagagggcc
     9841 gtcgcgatat cggccatgcc catgggcatg cccgggtgac cggacttggc tttctggacg
     9901 gcgtccatgc tgagggcacg gatcgcgttg gccatcatct gagcagaggc ttgagaggtc
     9961 atagtatttc tgtgagcgga ttgaagaaaa ccggagggaa gaaaccccga aaaggcaagg
    10021 cggcgacctt ccctaaggtt tgtacgccgc tgtctgactt aatctattat tttagcaaca
    10081 aaaggagggg gccaaccgag cgtgaaacca cggagctcct ctccgcacat ggattcggaa
    10141 tgcggaaacc cgcatgtccg cagacttccc atcctgtttt acccaattgc ggcttatatc
    10201 tccgtccttc agggcggaga tataagccgc gtgtttttga tgaacgctag tatcatatag
    10261 gcatgagtat tcgccaaggc ttttcgttca aactgaaacc caacggagct caggacaaaa
    10321 agatgtcccg cttcgccggc tgcacgcgct gggtgtacaa ccaagggctt gcatggaatg
    10381 aagagcgtcg tacagcggat cctgcctttc atctcagcta tgcgaagctc tgcaccgaac
    10441 tgctggtttg gaaggagcag aatccctggt tgagggaaac gcactctcag gttctccaac
    10501 agtcgctgaa ggatctgatg gacggcttcc agaagttctt caaaggtctg acggctttcc
    10561 cgaagaagca caagaagttc gtgaccacgg actccttccg cttcccccag ggcttcaaga
    10621 ttgatgaagg acgtcggcag atttatctgc cgagcatcgg ctgggtcaaa tacaagcgaa
    10681 gccgcttcat tcaaggcaag gccaagaacg taacggtttc ccgacaaacc gacggatggt
    10741 atgtttccat ccagaccgag tacgaaatgg aacctcccaa gcatgccggc gacagtatcg
    10801 gcattgacat gggatgcgtc cgtttctgca cgctttccga cggcacgttc ttcgaaccgt
    10861 gcggcgcact caagaaggcg cttaagaagc tcgccaggat gcagcgcagg ctcgcaagaa
    10921 tgcagaagtt cgagaagaat tggcgcaaac agaaggcagc gattgcaagg ctacatcagc
    10981 atatcgccaa tgtccgtcgt gatttcatcg aaaaggtctc gctgaatatc tgcaagaacc
    11041 acgcagagat tttcagagag gacttgaaga tcaagaacat gacggcatcc gcgaagggaa
    11101 ctgttgagga gcacggcact cgcgttgcac aaaagagtgg tctcaaccgt tccattctcg
    11161 atcagggttg gaggatattt ttctccaagc ttgattggaa ggcgttgaga cttggcggac
    11221 acgtacatac ggtgccgccg cagaatacaa gtcgcacctg cccgatatgc ggctgtgttt
    11281 ccaaagacaa tcgcaagacg caggcgttgt tcaaatgcgt ttcctgcgga catcagggaa
    11341 acgcggacgt cgtggctgcg atcaacatca aagagcgcgg acgaagctcg ttagcctgtg
    11401 gggagtctga gaggaatgct caggctcaag tgaatttggg tcgatctaaa aagccggcgc
    11461 cgaatcagca gcaggaatcc accgaagtaa tcagcagcgc aagttgctaa cgcagtagag
    11521 aatctccgtc gttcacggcg gagagaacgt caaggagaac tcatggccgc accacgcttt
    11581 tacgttgaag tcgagaccga agcgctcgac aaggctctcg ccgagcaggc ggaagtcgtc
    11641 ctgccgccca aggccgccca ccacgcggga cgcgctcttc gtctgcgaac aggcgaggag
    11701 acggtcgtct tcaacggaac gggacgccag tggcggggcc gcatcagatt cgatcaggac
    11761 ggcgcctacg ttgcgctcga cgcggtcgag acgcccgagg tcgagccccc cgtgcgcatg
    11821 acgctcgtgc aggcgctcgt atcacctgaa aagcttgact ggatcgtgga aaaggccgta
    11881 gagacgggcg tctccgaaat cgtgctgacg cccgccgcgc gcagcgtgac gaagctcgcg
    11941 ggagaacgtc tcgaaaagcg ccttcagaag cttcgcgaca tcgccgtatc ggccgccgaa
    12001 cagtgcggac gcaacgtcgt gcccgaaatc cgcggcgcat cctccttcaa ggaggcgatg
    12061 ctcggcacgc aggcggaccg caggctcgtt ctggctcccg gggccgcgca cggcacgagg
    12121 ctgacgggcg aggagaagtc ggtggcattc gccgtgggcc ccgaaggcgg attctcgcct
    12181 gaggagatcg atctggccga agaggccggc tggctcgccg tcctgctcgg cccgcgcgta
    12241 ctgagaaccg aaactgcggg catcgccgca gccgtctggc tcaacaccct gacgggcgac
    12301 tacggagagc ggtaggcgtc aggacgcctg agccttgccg cgcaggtctg cattgcgggc
    12361 gcggggagcg ccctcggtcc gttcggagag tttggtccag tacgcaaagc ccagcttggc
    12421 ggcattgcca gccccgcctt ccgacgccga cttgccagcg cggtgaagct ttaccgcagc
    12481 atcggtcctg ccttcggccg caacggtaag aaggccgtgc tcgcgcatca cgcgagctcc
    12541 gtaccctcga tcgtcctcga gattggccgc gccgctgtac cacttgaggg cgcgcctgac
    12601 gctgcccgtc cggcggatca gataggaaag aatgtccgtg ccgaccacca tgttcgcata
    12661 gggatcgaag gcggcctcgg ggcctccgaa ggcatcgaat ttcgcctcat gcacgctcgt
    12721 catgacctgc atcaggccct ctgcgcccgc attcgacctg gccgtcggct tgaagctcga
    12781 ctccgttccg atgacggcga gaatgagcag gggatcaaga tccctggcct cgccgatctc
    12841 cacggcccag tccgtgatct ggcgggcatc ccgcatcgag acacggaagg ttctgctgat
    12901 gtattccgcc actagatggc gggggctgcc ttcatgaatg acgttgtcct cgaccgtcag
    12961 ctcctccgag gaggccgtcg cctcctccct cgccgcagca ggcgcatcga gcagcgacag
    13021 ggacggtgtg acgccggaga gcacgccctc gcgctgcctg agatccgcac gagcgttcag
    13081 gtcctcctgc gcatacgaaa gcgcgggcgc agtgcttgca tagtcggaat ccgaccagaa
    13141 cgcggctccg ccgagcgtca gggcgagcac gctcaccgaa acaagatgcg agatccggaa
    13201 ttgtcgaggt tgttttctga aggtcatgaa gtctgtcttg tgagccgcgc gtccatgccg
    13261 gaggggtggc aggcggcggg gagagcggat gcttctccag ctcccgcagg agcctcaaat
    13321 cctttgtagt gttcgatcag ggggcgatgc tagcaaaaaa gctgccgccc tttcaagttt
    13381 tgaaacgccc gaaagcatgg tctgcctcaa gcttcgggca ctttatacga ggtatttacc
    13441 ctctccgggc atccgatcct gcggggatac aatgatctga cccatcgttc acggattcat
    13501 cggacacacc atgaagcgca gacccattca ccgcggcggc attctgtccg ccggctacga
    13561 cgccagagcg cgtcatctcg acgtcgagtt cgacacgcac aggctcgtgc gcgtcgagaa
    13621 catcggtcct gaagccgcgg accgctttct caactcgggc tcgcccggca gctactggcg
    13681 cgacgtcatc gaggagggct acaccatccg cgagatctcc aaagcggagg gagacgctcc
    13741 cgccgcaggg aaaaagggca tggatgacct gaagcgcctt ttcggcgatc tttgaagaga
    13801 cggcgctcga ccgcgcccgt cccccgctct tacgacgcga agaagaaggt ggcaagcccc
    13861 aggaagatga ggaagccgcc cgagtcggtc gtgaaggtga gaagcaccga tccgcccatg
    13921 gccggatcac ggccgaagta gcggaggatg agcggcacgg ccagaccgac cacggcaccc
    13981 tgaataatgt tgagcaacat ggcgaggaac atcacgaggc ccagcatcct gccctcgggt
    14041 gcatcccagt agagtcccca ggcgcacaga cctgcgatcg ccccccagag gatgccgttg
    14101 atgatggaaa ccaggacctc cttcttgatg aggtccgcct tgtttgcctc ggtcacctgc
    14161 cccatggcga gggagcgcac cagcagcgtg agcgtctggt tgcccgagtt tcccgccatg
    14221 cccgccacaa tgggcatcag ggcggcgagc gcgaccacgc gctcgatcgt gccgtcgaag
    14281 gccgaaatca cgcgagacgc gaagaatgcg gtgcacaggt tgacgcccag ccagagccag
    14341 cggcttctgg cggcctccca cacgctggcg aagatgtcct gctcatcgtc aagaccgacg
    14401 gccgcgaagg cgtcctcctc gctttcctct cggatcacgt ccacgatctc gttgaccgtc
    14461 agacggccca ccagacgtcc cgcctcgtcg accacgggag cgctcacgag gtcgtagcgc
    14521 tcgaaggcct gcgcggcgtc gctcgagtcc tcaagaggat tgagcgtcag catgtcggtg
    14581 tgcatcaggt ccttgacggg gaccgcggga tccgtcgtga ggatctgcga aagggacagc
    14641 gtgcccttga gatgccccag acgatcgacc acgaagacct ggtcggtgtg gtcgggcagc
    14701 gtctcgaagc ggcgaaggta acggagcacg agctcgatcg tcgtctcctc ccggatcgaa
    14761 acgatctcaa agtccatgcg cgcgccgacg ctctcctccg gatacgacat ggccgctcga
    14821 agctgggcgc gttcctcgtg ggagagacct tcctgaacct gcgccatcac gtcgggcggc
    14881 aggtcgtcca caaggtcggc gatctcgtca gtgtcgagag tctcgacggc atcgacgagc
    14941 tcgtcgcgat ccatggcgtc gatcagggtt tcacggacgc cgtcgttgac ctcgacgaga
    15001 atgtcaccgt cggactcgct cttgacgagg ttccagacgg ccagacgttc gtcgagaggc
    15061 agggcctcaa gaatccaggc cacgtcggcc ggatggaggg gttcgagaag ttgcctgagc
    15121 tcggttgcag cctcgtcgct gatgggcagc gcctcgtcat cctcgtcccc cttgcgctcc
    15181 acgccgccgt ggtgctcgtc ctgatccttc tcaaggatct gctgcaccca ctcgagggcg
    15241 cgggaggcct cctccgagtc gcggacaagg tggccctggc cgtcatgcag ctcgtcgtaa
    15301 tccttcagac tgtgaggcat caccttctcc gaaacacttg aagagcccct gaagggaggg
    15361 actcggaacc cgtcatgact tttcgcaggt gggagcctac cacgagtatg aagccttcgg
    15421 gtaggggctc cctgaaattt attggcgcag gcggagcggg aatcaccgcc ctgtcacctg
    15481 cgtccgggac tcaggaagcg gatgactcgg acacggtgtc cccgccctga gcttcctctc
    15541 ccccttcaac tgcggcctgc tcttcaagag ccgcctcctc gtcctcatcc tcgtccgagg
    15601 tgtcggcggt ttcgtcaaga acacgcagaa gcctggaatc catgacgagg tccacgaggt
    15661 cgcaccccat gatctgcaac aggaccttgc ggccgcgagg cagatcctcg ggaagaccgg
    15721 gcacgcgctg catgaaggga agcccgtcga tgcggaccag atcgcccttg acgacgatgg
    15781 cctcgatctg gccgacgctc tcctgcatga tccagcgcag ggaccagtag cgctccatgc
    15841 gggtctggaa gtcgccgtag agcgtgtaga tgttttcaaa ctggctcacg atcgtgaaga
    15901 gctcgacgtc gttgccctga taggcgggcg gctcgccaag cacggccgtg atcatctggc
    15961 gctggttgac gaggtcgacg tagcggcgca gcggcgaggt gctccacgca tagcgcacca
    16021 cgcccagacc gtcgtgcggc cccggggtcg tgctcatgcg cacgcggccc atgcgctgcg
    16081 aacgatagat gccgggcgtc ccgtgttcct ccatccagag gccccaggtg ctgttggcga
    16141 aaatcatgag ctcagcgaca agcaggtcga gcggcgcgcc gcgacggcgg cccttgacgc
    16201 ggatgagcgc atcctcgccc tcgccttcaa gcgcgaagaa ccagtcgaca cggcccacgg
    16261 gttcgggacg gcccctgacc tcctcgcggt ccttctgaag ctttttggca aaacgccaga
    16321 gccagcagat ctcgtccgcg aactcaacgt cgagcgtgcc cgactgaatc gcctcctcgg
    16381 tcacgagcga gtcgatcttg tcgtagcgca gattgcgcgt gacgctcacg cgctcaagcc
    16441 tcgtctcggt cttctcaacg gagaaggtct cgtcgtccac cgtcacgtag agcgagaggc
    16501 agggcaccgc acggccctcg tcaagcgaaa aggccttgat ccacggatcg ggaagcatcg
    16561 tcgtcttgag ccccggcgca tagaccgtgc tcatgcgggc gcgggccacc ttgtcgagcg
    16621 gatcgtcgcg cagaatgccg agcgcaggcg cggcgatgtg aatgcccact cgcgtgcgac
    16681 cgccctcgag atgcacgacg ctcgccgcgt catcaacctc ggtcgtgtcg gagtcgtcga
    16741 tggaaaaggc cctgacgtcc gcaagcggat actgcgacca gtccgcctcg ggcggcagag
    16801 gcaggtcgga ggggaagccg cggccctgcg ggaagttcat ggcgtagaag ctgtcgacgt
    16861 gccagcgcca tggcgaggcg atgccgccga gcgagagcat caggcgcagc ggcgtctggc
    16921 gcgtttcggc ggaggcgtcg ttcagggcct tccactcgat accgttcttg tccggccgaa
    16981 tgagaagcgt catggcgttg cggcggatga cgtcgggaag cgttccctcc accatctcgg
    17041 ccgtccaagc cttgcgctgc tcctccagac ggcgcttctt ctcaagtgcc tcgagcgcct
    17101 tctgaaggat gtcctcgggg gcgcggcggt agccgccgcg gccctttcgg tagaagtaga
    17161 cgggattgcc gtggagcgcc cagagaaggg cggccttttc aacagggccg gccgagccgc
    17221 cccagtagtc ggctgcaagg tccgcgtacg tgaactcacc ctcgggcgca acctcccaca
    17281 ggaaggcggg atcgagctcg ggcaccaggg cctgagcacg ggtcacgagg tctgcgggag
    17341 caggctgctc gaactcgaag aacgagtgcg aggccttgat cttcgtccgg cgtcccgtcg
    17401 ggagctgcac ctgataggcg ttgcctgcct ggctgagcac ggtgcccgcc ttgaaggagc
    17461 cgtcttcttc aaaaaagaga ttcatgaatc tgtcgcttta catccgctct gggcgaatga
    17521 gtgatggtcg aggggaaaac gcccccctat tgtgccaaaa caaggcgcgg tgcgcccgcc
    17581 tcagccttca ttgagaatga agtccatgat gcggggcatg accgccccga tgttcgagat
    17641 gcggtggtcc ccgccgggaa taatcacctg cctgcaggcg gcataggccc tcgcccccgc
    17701 ctgccagtca agcgtctcgt ccgccgttcc cagcacgacg agcgtgcgtc cgggatcgac
    17761 cgccagaggc gacacctcct cggcgagcgc catgagttcg tcggcggccg aggccttcac
    17821 ctcgatcctc ctgtccgtgc cgtagatcgt ctgcacgccc acgtgcctgc ccacgaacac
    17881 ccagggattg aagcagggat tgagcagcac gcagcgaagg ccgtgcctga cggcagctcg
    17941 cgcggcccag aatccgccga ggctcgaacc cacgaccgtc agaccgtcgg cctccgcctg
    18001 cccgaccagt ccgctgatga tcctgtcggc ctcaagaggc gggacgttca gatcgggagc
    18061 gaacacgcgc acgccatcgg gggccgccgc cctgagcacc ctcactttgt tcgactcggg
    18121 agaggacaga aagccgtgca gatagagaat ggaagtcatc gcccgtgcac ccttcactgc
    18181 gcttcgcgcc tgcaggcgag tcccgcaagg agcttgccgt gaatgccgcc gaacgcgccg
    18241 ttgctcatgc agaggaccgt gtctcccgga cgggcctcct tgagaacggc ctcgacgagc
    18301 acgtcaaggt catgcgtgca gaaggcgcgc tcgcccatga cgtcgagcac ttccgcgggc
    18361 tcccacgcca cgccctttcc tgcgaagcag aacacctcgt ccgcctcgtg cagagaaccc
    18421 gcaagcctgt ccttcatcgt gccgagcttc atggtgttgc ttcgcggctc gagcacggcg
    18481 agaagcctcc ccgacgtcat gcggctcctg acggcatgaa tcgtctcggc gatggcggtc
    18541 ggatggtgcg caaagtcgtc gatcacggtc acgccgtcct caacgcccct gacctccatc
    18601 ctgcgcttga cacccgcaaa ggccgcgagc gcctcgagcg acttttcggg agggacggcg
    18661 acgctgcgcg ccgcagcaac ggcggccagt gcattgagct cgttgtagcg gccgggcatg
    18721 ccgagctgca gacggccgaa gctcctgcct tcaaacgccg cctcgccgtc atcggaaagc
    18781 gaccagccgt cctgagagtc aaagcgctct agagcgctcc agcagccctt cgcaagcacg
    18841 cggtcaagcg cctcgcagcg gccgttcgcc acgacgagac cttccgaggg catgatgcgc
    18901 acgagatgat ggaactgctt ctcgatggcg gccagatttt caaaaatgtc cgcgtggtcg
    18961 tattcaagat tgttgaggat cgccgttctg ggacgatagt gcacgaactt gctccgcttg
    19021 tcgaagaagg ccgtgtcgta ttcgtcggcc tcgatgacga agggcgacat cggggcgact
    19081 cggccgagcg acgcggagcg cgggctccac tgcggcacgc cgccgatcag gaagccgggc
    19141 tcgagtcccg cctccttaag gatccaggca agcatcgacg tggtggtcgt cttgccgtgc
    19201 gtgcccgcga ccgcaagcac gtggcggccc gcaagcacat gctcgcagag ccaggcggga
    19261 ccggacgtat aggaaagccc cctgttgaga atcgcctcga gaagcggatt gccgcgggag
    19321 atggcgttgc cgatcacgaa gacgtcgggc cggacggcat caagctgcga gacgtcatag
    19381 ccctccatga tctcgatccc cgcattgcgg agcgcgtcgc tcatgggtgg gtagacgtgt
    19441 gcgtcgcacc cggtcacctt gtggccggcc tcgcacgcga gctgggcgac gccgcccatg
    19501 aacgtgccgc agatgccgag aatatgaatg tgcatgtact tgttcctcaa accagaatgg
    19561 aggcggaagg tgcttcattc taccgcgcgg aggatccccg ccctgaaagc cgccgcacta
    19621 tcattggctc atcgtccatg acgcctcacg caccttcatt ccggagacat ccgtgcaacc
    19681 caatgacctc ccgcgctcca aagaatcctc agccgccgct caggcggccg tcctgatcgc
    19741 atcccaccgc atgagcaggg atcgggccgt cgagaccgtc gttcgctcga cgggagttcg
    19801 gccgaccgat gccgccgtgg cgtccgagct caggcgctgg ttcgcctgct tcgatcccga
    19861 ggggcacaag gcgcttctgg acaagaagcg ccttgcggcg ctctccgtcc tgtcgtccct
    19921 ttccgactgg tctcccctgc tgacgggatc cgttcttgca ggagacgccg tcgaggacga
    19981 tcctgtgcgc atcctcatcc gctccgacga cgaaaaggcc gtggagctcg cgcttctcgc
    20041 catggatgtt gaatacgaac ccgtggagtc ctccgaacgg ggacgctcct tcagaaccgt
    20101 gcttcagact gaatgcaggg gagaggacgt cctcatcacc gtgacgaact cccacagggc
    20161 ggcgaagccc
//
LOCUS       DBMDTI010000012        19834 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_910_length_19834_cov_2.040795, whole genome shotgun sequence.
ACCESSION   DBMDTI010000012 DBMDTI010000000
VERSION     DBMDTI010000012.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 19834)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19834)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19834
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_910_length_19834_cov_2.040795"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            228..1415
                     /locus_tag="ACZPB6_01570"
     CDS             228..1415
                     /locus_tag="ACZPB6_01570"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1183199.1"
                     /translation="MKPTSKNLLLLPIGMHRYERGLYVRVTATSRSWVFKYQLNGRRR
                     EMGFGPVEGQAVSSILGKASAARFQIDRGIDPLAEKEARRAEAVAEAKRAKMVTFAEF
                     APKAVERIFKIRKFRGDNTECSWLKTLDRLVKLLGKHKLDEISTRTVVEALEPAWEDS
                     ASASEELQGRLSNILDVAVTEGYIELNPAAWARLKMWLPSPSTLRRAKPAKHHAALSA
                     EDLSSLAQRLWADGSKNSLAILFGILTVGRRQEYLEAKWSEIDTEELTLSVPPERRKD
                     GKLVPHVVPLSRQAQRLLERLDTSSEHLFPGRGCAHISPTSLSNVAASLSTEDKPFTL
                     HGIRSTFSDWCARNEKNFVVSEKCLMHAVGNAVFRAYQRDDLLEQRRKLLQEWADFLL
                     PNV"
     gene            complement(2138..2599)
                     /locus_tag="ACZPB6_01575"
     CDS             complement(2138..2599)
                     /locus_tag="ACZPB6_01575"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HIR1183200.1"
                     /translation="MEYKIFTAYPVELAADFIEQWEGFRERAYRCPAGVLTIGFGHTG
                     TDVHEGDTITYHEAYDLLVEDLKRYCAGLARWVNVKVTANQFVALLSLAYNIGVDGVV
                     HKCPKLLRALNAEDFDEAARQFLDVTKANGKVLPGLTRRRKAEAELFLREE"
     gene            complement(2589..3095)
                     /locus_tag="ACZPB6_01580"
     CDS             complement(2589..3095)
                     /locus_tag="ACZPB6_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR1183201.1"
                     /translation="MLNTLQSLFPQGAERAFAYIGGVVGSLASFAFGDVGPLLTWLVI
                     FCVADFFLGSAVAIYRHEWSSHKNYMGILKKALMFLIVALSHGLDMMFEPLIHFQIFE
                     SITICAYAAGEFGSLIKNLEYGGFGGVVPPVLRRVIHTLNERLEEKADAALESRGLSK
                     KKESNDGI"
     gene            complement(3154..3363)
                     /locus_tag="ACZPB6_01585"
     CDS             complement(3154..3363)
                     /locus_tag="ACZPB6_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024139.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1183202.1"
                     /translation="MALNLEKLVTRAQIAEAFGVSTKTVTCWVAAGKFPPPIHVLGTS
                     RWREKDVTEYVARRETIARRGFAVR"
     gene            complement(3366..4772)
                     /locus_tag="ACZPB6_01590"
     CDS             complement(3366..4772)
                     /locus_tag="ACZPB6_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HIR1183203.1"
                     /translation="MRKFTPRPYQALIIDAILSRKRIAIWAGMGMGKTVSTLTAIRIA
                     QTMGEGPALVIAPVRVAQSSWPDEVEKWDHLRGMKAVCLCGEKRKREALARQKADVYA
                     ISYGLLNWLVDFWGDKWPYKIVVADEATRLKSFRTRQGGTRAKALATVAFKHVDRFIE
                     LTGTPAPNGYLDLWGQLWFVDAGKRLGKSMTAYQQKFFRPVRVGAAAFAVRWEILPGS
                     ETRIQEALSDVTMKVNAEDWFDLEEPIYSTISVTLPAEARKIYDDMERKLYAEIDGQE
                     VETANAATKTSACLQIASGNLYYENADGMLPEAKDAKPYLVLHSEKVEALRSIVEEAA
                     GMPILVAYNFRHELEVLRAAFKEARVLDKNPKTIREWNAGKIPMLLAHPASCGHGLSL
                     QDGGNILVFYSTNWNLEEHDQMVERIGPTRQAQSGHPRSVFVYTIVAKDTLDEAVLER
                     IATKRNVMDILLEKKNGG"
     gene            complement(4772..4909)
                     /locus_tag="ACZPB6_01595"
     CDS             complement(4772..4909)
                     /locus_tag="ACZPB6_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183204.1"
                     /translation="MVNPDNWKLMLPVVALLPALFLPFPWDAVMWASTCVAVWLYFKR
                     D"
     gene            complement(4903..5229)
                     /locus_tag="ACZPB6_01600"
     CDS             complement(4903..5229)
                     /locus_tag="ACZPB6_01600"
                     /inference="COORDINATES: protein motif:HMM:NF020354.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HIR1183205.1"
                     /translation="MTPEGKIVAFLVRRCKELGLYQRKLAYEGRRGAPDRLIVGFDCF
                     AMIEVKAPGQKPRREQVREIEVLRNGGIPVYVCDSTDAVEGILLDLRERTDTNRLFAL
                     REGPQW"
     gene            complement(5230..5724)
                     /locus_tag="ACZPB6_01605"
     CDS             complement(5230..5724)
                     /locus_tag="ACZPB6_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183206.1"
                     /translation="MTTKFKPEEWWPVLALHACGYTMSVYEAKGLLTLLSKHRCTANQ
                     WSDGRFEERGQPAFLASAISTLGKALEYSIRNRTDLCAPAFTVGETFAIYLALAGKSH
                     WAMRFLDAGVASLMSCFYRALNPVMKGATPEDAETILDSRIEPVIWQIGRDFYLAAGG
                     KKEK"
     gene            complement(5708..5959)
                     /locus_tag="ACZPB6_01610"
     CDS             complement(5708..5959)
                     /locus_tag="ACZPB6_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183207.1"
                     /translation="MKKFRAMTREGLKKEYGLTDEEVDEAERGPREPCFGFGIGGEPS
                     EVGLFFRLKLHRGDGEVFEVALACDLFEDNGDEEYDYEV"
     gene            complement(5946..6290)
                     /locus_tag="ACZPB6_01615"
     CDS             complement(5946..6290)
                     /locus_tag="ACZPB6_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183208.1"
                     /translation="MNAFAHWFDAAQLERERLKKLNPFYRNSLAYKNRARQIETADTY
                     IGRHIGSLIVKNVDGFDVNRADGQTTTAFLCDCACGRTDVRVPCTEFSRGVSADCGGP
                     AHKRRRRKSEKV"
     gene            complement(6620..7039)
                     /gene="dut"
                     /locus_tag="ACZPB6_01620"
     CDS             complement(6620..7039)
                     /gene="dut"
                     /locus_tag="ACZPB6_01620"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012895.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR1183209.1"
                     /translation="MEIKVKKLHPEAKLPWRGTADAAGLDLFCTKSTVIPQGETVKIP
                     TGLAMEIPKGYCGVVYSRSSSAVSGLIITPLIVDADYRGEIMVIVRNGTDGAFEVKAG
                     HRVAQIKIEKLEDVEFVEADELSETARGTGGYGSTGK"
     gene            complement(7023..7283)
                     /locus_tag="ACZPB6_01625"
     CDS             complement(7023..7283)
                     /locus_tag="ACZPB6_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183210.1"
                     /translation="MRYDHKPRIEIPVCALHGDEESLVVKPRVVKCEPGKFPEVVVDF
                     GDVKGVTLSQLHKLEDLITRTYDYRRALYCHPELRIHHGNQS"
     gene            complement(7280..7951)
                     /locus_tag="ACZPB6_01630"
     CDS             complement(7280..7951)
                     /locus_tag="ACZPB6_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183211.1"
                     /translation="MKKIVKKRVGKKPFKHRRVAAGAYLYRVLPELDDAMLGNIELWA
                     RVPLDAIKRGEGTEENVSNVQTALTFGFVLAAAFENKTEIQMLIQLAEAGIGGACRCI
                     RNGEPEGIWRELFEPVDAALGVISDMQRTVEREVLVRCIDAIKVHNFSLRANPKSAFL
                     IPADERAKRFMNRPGLAYINGKARSGYLQKNDTMNRIEWVSPNEELQIPITKYTLIVF
                     AEPLK"
     gene            complement(7948..8406)
                     /locus_tag="ACZPB6_01635"
     CDS             complement(7948..8406)
                     /locus_tag="ACZPB6_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183212.1"
                     /translation="MFDKTTLAHTPFTFGVTPRMAKDVCALAHETLFKESIEPALLVF
                     VRPAIEHFEEVTKREHVEDEYNVHCRLVDILALNEAITTDRGRYKDAFEFIRRLRRYV
                     ISALLGTLYGTLNDIVRTLDQKDVVALYTLLHVKTEEIFSSILTEKVATA"
     gene            complement(8411..11404)
                     /locus_tag="ACZPB6_01640"
     CDS             complement(8411..11404)
                     /locus_tag="ACZPB6_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012688.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR1183213.1"
                     /translation="MMANYSFHDLESYSETPIKFGSHRYAQDCEIMLWAYALNDEPAE
                     VWDLTSGAPMPAKLKKILDAAMAGKCYTVWHNGMNFDTVVLKAHGYDIPLRMIVDTMV
                     MAYQHSLPGALGDLSAVFGLSEDVAKDKDGKRLVQLFCKPRPKNSTYHRADRTTHPED
                     WAHFVEYCRLDVEAERALFKKMPKFNLSKHERDLQILDAEINRRGMLMDVELANAAVA
                     IAEETRVLLARRTREQTDGAVEAATQRDALLSYFESEYGVTLKTMTKAEVEKRINDDT
                     IPEPMRELLRLRLQSTKTSVQKFAMLAEAVGRDGRLRGCLQFRGASRTGRFSGRLFQP
                     QNLPRPTLKQDEIDFCIDAAKAGTLTALYDDPMEVLTNCLRSEIIAPAGKKLVVADYS
                     NVEGRVLAWAAGEEWKIQAFRDFDEGHGHDLYKLAYSRAFGIKPEDVSKKQRQVGKVL
                     ELALGYGGGAPAFARFAKAYGIDLHDMAKNVRETISPMTWREAEDSYEYFLKKNLTGG
                     LHRDVFIACDALKRAWRATNPKICALWGDLGGAVMKVLEDPKLVLTVGPATVSRTAGF
                     LLVELPSGRKLCYPSPAPSDIGKADSFTYLGINQMSRKWVRIESYGAKACIAKGTPVL
                     CRRGWTPIEDVTPLDEVWDGVEWVHQEGAIDKGTKQTMTAYGVRMTPDHKVLTTEGWC
                     CASQSKGFSRADCRIPDGYGICRLGRAPHAMEVPVLCLREDGCSELQSPRALQKGQEP
                     RLMRMQKVEVHRGKDLSARHERDTALERVALHERALRDSDRASLEELRGARNHCLQRV
                     ERIIREVLRGHGTDLQERATARPNRQREGLLEGQLPLGNRSRKLQQPAKDGAHRREAA
                     CGLGTGNRDWLDNSPLPTDARMPEGALVRQAQRDEQVYDLVNCGPRHRFVVLGEEGPL
                     IVHNCENLIQAIACDLLCDALLRLDHAGYKTVLTVHDEAITEAPDTADYSLEKMSALM
                     TELPTWATGLPLAAAGYEAYRYKKD"
     gene            complement(11401..12114)
                     /locus_tag="ACZPB6_01645"
     CDS             complement(11401..12114)
                     /locus_tag="ACZPB6_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183214.1"
                     /translation="MAEKFSREKMEALFSGKKYGHVTVGNFLREDDKVTKSHRARWIV
                     TATCGYCGHVFEDFFDNIRYKANPSCGCQNRISNWNRSRELDRMEGTARAKRREEREA
                     KAKAKAEAKAKAEARKKEAKAKVTMKRVNKKAEKSAAVKKAEAKTAALAKQAQRASIY
                     VKVDRGVCIGDGIELTLARLHQLWELLDKADCCPAWRENSQSFCLWGIRNGYARGKVL
                     VKREDAKPWHPLNCKWEIE"
     gene            complement(12352..12909)
                     /locus_tag="ACZPB6_01650"
     CDS             complement(12352..12909)
                     /locus_tag="ACZPB6_01650"
                     /inference="COORDINATES: protein motif:HMM:NF022444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ssDNA-binding protein"
                     /protein_id="HIR1183215.1"
                     /translation="MKLNISVRCAYPHVFEARENAFAKDGTKKYECTGLFEANGAVHK
                     AILDVMKAVADQKWGAKGQRYYEEALENKNTRLIQKDADLGLMKVTARRRETDGAPAV
                     VDQRLRAIAPSQGIPYGGCWINMRIDVWAYENNGSRGFSATLLGIQFVRDGEALGGAS
                     AASAEGFEVLETDDKEAAVSDDPWA"
     gene            complement(12906..14171)
                     /locus_tag="ACZPB6_01655"
     CDS             complement(12906..14171)
                     /locus_tag="ACZPB6_01655"
                     /inference="COORDINATES: protein motif:HMM:NF022379.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HIR1183216.1"
                     /translation="MTHALLSPSACHRWRACPASVVLTKDLPDESSDFAEEGTRAHRL
                     AELKLLGTPQFSGTAVTARRRTDAETEEFKTLWADAPDEMQAAVCVYARTIADLIGEG
                     KPSYFMVEHPVDVSSVTGEAGAHGTVDCVVRVGEHLYVVDLKYGRGVKVEARCNEQLS
                     IYALALCDELDPFGDDPVTTIHIVIVQPRLNHIDHWTTTTGGLRSFAEDVKARGARAL
                     ALCDGAATESADFGPSESVCRFCRAKGTCPAIRGAVVEAFKAEPAIVDAAPQVVEALS
                     IPVPTEAEALSRALNVLDLIDQWTSAVRAEALRQLEIGNKIPGYKLVQGRAGIRRWAD
                     AKEAEAKLKSMKVPEALRYEKKLISPTAAAKLTKAKAGEAPVLTERQWAKLEALITRN
                     ESKGAVVPESDPRPALDMGEGFKVIEENK"
     gene            complement(14175..14579)
                     /locus_tag="ACZPB6_01660"
     CDS             complement(14175..14579)
                     /locus_tag="ACZPB6_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183217.1"
                     /translation="MPLENILSDLTAEIKALRLTIAAGMKAPAAFTVEAAAAAPVAAP
                     APINPAPIEAAAPTAEAAPVQSEAAAPVSDTVAADQAPAAAESAQTPAKTEQKTPETQ
                     TEKEKTDESRSTAPDKDELPPVTDAVTARLEG"
     gene            complement(14618..14917)
                     /locus_tag="ACZPB6_01665"
     CDS             complement(14618..14917)
                     /locus_tag="ACZPB6_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183218.1"
                     /translation="MVDIKPLSRPPFTARGRLSARAAAALQWFYRWKSVRPVRLRFRT
                     PGGPAAGLAGRCAYLDYFGVPYRRGHDAGGDYIELPPAAFSMIHGLAGLYGFSLE"
     gene            complement(14972..15166)
                     /locus_tag="ACZPB6_01670"
     CDS             complement(14972..15166)
                     /locus_tag="ACZPB6_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183219.1"
                     /translation="MLYVLAIKQRAYPGARWQVLPARFSSAGAAHAAQRALRQRFHLL
                     QALYLTLDTAARCPERFQLN"
     gene            complement(15183..15869)
                     /locus_tag="ACZPB6_01675"
     CDS             complement(15183..15869)
                     /locus_tag="ACZPB6_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta clamp domain-containing protein"
                     /protein_id="HIR1183220.1"
                     /translation="MTVISLSIRSYYLLSAALEFAPKDSIKCFLNAVHIAPQGGAAHV
                     VATDASALFIGRAEAAPSVVDTAGALQPFEPVTIHRAPLAAALKAVKTAVKNADTLEI
                     EIDEDRRSGRLRVFNFRLDRTGSSAFSAPISLVDTKYVDYRRIIPTYTDPAAPIPVVS
                     CKYLTAVTKAAAALRPARERADLSAEIVNQMQAVFALADDAAAIVMGIRLDDRRRPSY
                     ERTAAAIMAD"
     gene            complement(15936..16199)
                     /locus_tag="ACZPB6_01680"
     CDS             complement(15936..16199)
                     /locus_tag="ACZPB6_01680"
                     /inference="COORDINATES: protein motif:HMM:NF046163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6926 domain-containing protein"
                     /protein_id="HIR1183221.1"
                     /translation="MRTEILSFNLPAWAVCPLENSDGTGLNPEDLEAIERFESELEEH
                     RARLHASSYCFSWPSEECPHFCSAPAFGLPADCYATDVVFFLN"
     gene            complement(16211..16663)
                     /locus_tag="ACZPB6_01685"
     CDS             complement(16211..16663)
                     /locus_tag="ACZPB6_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183222.1"
                     /translation="MTEFNTTRAPSVSDLRELFAARPAVLSAINEAFKLSGGFYVRAA
                     RWHSADEAAEVVLQAVGAVDCGAYDDETGEYHNEPLSVEVFTADNGNRIIDAVLACGG
                     PTVTARYESRYGSITVEAHWGSAFIELYGFEGDCPLRAYLSNSADFAE"
     gene            complement(16682..16846)
                     /locus_tag="ACZPB6_01690"
     CDS             complement(16682..16846)
                     /locus_tag="ACZPB6_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183223.1"
                     /translation="MMTAARFSLRCACYGLALYACYGVKYWPVWPGTLVFLGLLALAL
                     AAHFAAESLN"
     gene            complement(16843..17028)
                     /locus_tag="ACZPB6_01695"
     CDS             complement(16843..17028)
                     /locus_tag="ACZPB6_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183224.1"
                     /translation="MTILLKINVARRLSGSEGRRPALSFSSYGEAFKASREIYIAGHN
                     ALDGRSYRQCLTCKECR"
     gene            18507..19232
                     /locus_tag="ACZPB6_01700"
     CDS             18507..19232
                     /locus_tag="ACZPB6_01700"
                     /inference="COORDINATES: protein motif:HMM:NF045246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit-like protein"
                     /protein_id="HIR1183225.1"
                     /translation="MAPRLRIERDGEYEIPSKLNPALRTRKYKEPHPDSEWRPWWKPR
                     KRPLGGAKNPMVKKIREQIEKELAMDEGELARIPRDAETGRVVKSYELFTALLYWVAR
                     GGTLRSFCIAVDLPYGTCCGWLEKEPEVKAQYKEAERISADALVEYAQELATNPYIVE
                     EVFESYDGEGNLIRKDVRRQDAVYARKLCVNFCQWLVAKRAPEKYGEKLKVETDVSMA
                     GAIVAARRRLAARDDDIDAEDIE"
     gene            19320..19751
                     /locus_tag="ACZPB6_01705"
     CDS             19320..19751
                     /locus_tag="ACZPB6_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183226.1"
                     /translation="MLSAPEIELLKMTQEVIKRMADNSAKTKTLFVGVTTAAIAFLKF
                     DPSVHNLLALLAYLVVTVALWETDARYLRLERLFRHQHHAIVDGSVPALELWRMNLSR
                     YEEESVGRIMFTNFTMWIYQIAVLVSALLLAKVGGSLVGVF"
BASE COUNT         3709 a         5751 c         6272 g         4102 t
ORIGIN      
        1 tctggccgtc aatcaagcca ctttgcctcg atggacttga gttcgccgcg gcgatccaga
       61 aggtcccaga gatagttcat cacgcgcgtg tagtgggtat tgtgtagcgg gactgagacg
      121 gactacaagg gacaaaaggc ttgaaagaca cgtactttcg atctcccatg tgtaccttat
      181 agggacaacg caccccttca gggcacatcc aagtgtatga gcgcagtatg aagccaactt
      241 ctaaaaatct ccttcttctc cccatcggca tgcaccgcta tgagcgcggt ctttatgtgc
      301 gcgtgacggc cacgtcccgc tcgtgggttt tcaaatacca gctcaacggc cgccgccgcg
      361 agatgggctt cggccctgtc gaggggcagg ctgtctcgtc catactcggc aaggcgagcg
      421 ccgcccgctt ccagattgac cgaggcatcg acccgcttgc agaaaaggaa gcgaggaggg
      481 cggaggcagt ggcggaggcc aagcgtgcca agatggtgac cttcgcggag ttcgcaccga
      541 aagccgtgga acgcattttc aagatcagga aattcagggg cgacaatacg gagtgttcgt
      601 ggttgaaaac gctcgaccgc cttgtcaagt tgcttggaaa gcacaagctc gacgagatct
      661 ctacgcgcac ggttgtcgag gcgcttgagc ccgcgtggga ggacagtgcc agtgcctcag
      721 aggagcttca gggaaggctc tccaacatcc ttgacgtcgc ggtgacggaa ggctacatag
      781 agctcaaccc cgccgcgtgg gcccgcctca agatgtggtt gccgtcgccg tcgacgcttc
      841 gccgtgcgaa gcctgccaag caccacgcgg cgctttccgc ggaggaccta agtagccttg
      901 cgcaaaggct ctgggcggat gggtccaaga actcgctcgc catcctcttc ggaatcctta
      961 cggttgggcg caggcaggag tacttggagg cgaagtggtc ggagatcgac actgaggagc
     1021 tgacgctctc cgttcctccg gagcggcgca aggacgggaa gctcgtcccc cacgtggtgc
     1081 cgctgtcgcg tcaggcgcag cgcctgctcg agcgcctgga cacatcgagc gagcatctct
     1141 tccccggaag ggggtgtgcg cacatatcgc ccacgagcct gtcgaacgtg gcggcctcgc
     1201 tctctacgga agacaagccg ttcacgctcc acggcatccg ctccactttc tccgactggt
     1261 gcgcccgcaa cgagaagaac ttcgtcgtct ccgagaagtg cctgatgcac gcggtgggca
     1321 acgccgtctt ccgtgcctac cagcgtgatg acctgctgga gcagaggcgc aagctccttc
     1381 aggagtgggc ggacttcctt ctcccgaacg tataaaagaa aagccccgcg aggcgtgaag
     1441 ccagcggggc aatttcttgc tcgtaaacga aggaggtaac cgcacggagt gagcatccac
     1501 tggcttggag atgtgacgcc tacatcccag tgcagctctc cggataaccg gagatctagg
     1561 cttgtgcggt gttgtttcct gtctaggaaa gcgaaggtca aaaaggcaaa tttttgaaaa
     1621 cccctccgct ccctagagcg agaatggtag cacaaaagag aaagccccgc gagctgtacg
     1681 gactcgcgag gcatttcccc attgttgacc acttctctaa ggaggagata acgctatttt
     1741 accagctgaa ctagggcgtc atgtacagcg gcgtttcgct gaagtagctc tccgccttcg
     1801 gcaagaagtt ccgaactttc tctgagaagg ccctcgcacc gggcgactgc ggctcgctca
     1861 tctcggcagg cagaggcgct ggcctttcgc actcgatttt ctgcggagcg ctgcaccctg
     1921 cgcaggtcag cgtcgagacg gtcagcatca cggcgaagag aagcgatttg gttttgtgct
     1981 tcaacgagtt ttgcatagtc ttttctccct tggttcgctc gtgccacggc ggcctcaagc
     2041 tccgacgtct gcacccgcgc ctcccacttg gcagtggagt gggcgtagcc gacgacgaag
     2101 ctgatgacac aagctccgat aacaacgaac ggcttgctca ttcttctctc aggaaaagct
     2161 ccgcctcggc cttgcggcgg cgggtcagtc ccggaagcac cttgccgttg gccttggtga
     2221 cgtcgaggaa ctgacgggcg gcctcatcga agtcctcggc attgagcgca cgaaggagct
     2281 tcgggcactt gtggacgacg ccgtccacgc cgatgttgta cgcgagcgag aggagcgcca
     2341 cgaactggtt ggccgtcacc ttcacgttga cccagcgggc gaggcctgcg cagtagcgct
     2401 tgaggtcttc cacgagcagg tcgtaggcct cgtggtacgt gatcgtgtcg ccttcgtgga
     2461 cgtccgttcc ggtgtggccg aagccgatgg tgaggacgcc cgcagggcaa cgataggcgc
     2521 gctcacggaa gccctcccac tgttcgatga agtcggcggc aagctcgaca gggtaggccg
     2581 tgaagatttt atattccatc gttggactcc ttctttttgc tgaggccgcg gctctcaagc
     2641 gccgcgtccg ctttttcctc taggcgctcg ttcaaggtgt ggatgacgcg gcggagcaca
     2701 ggtgggacaa ccccgccgaa gccgccgtac tcaaggttct tgatcaggga gccgaactcg
     2761 ccagccgcat aggcgcagat cgtgatcgac tcgaagattt gaaaatggat gagaggctcg
     2821 aacatcatgt ccaaaccgtg ggataaagcc acgatgagga acatcagggc cttcttcaag
     2881 atgcccatgt agttcttgtg gctagaccat tcgtggcgat agatggcgac tgcggagccg
     2941 aggaagaagt cggccacgca gaagatgacg agccaagtga gaagcgggcc gacgtcgccg
     3001 aaagcaaaag atgcgaggct tccgacgacg ccaccgatgt acgcgaaggc gcgttctgcg
     3061 ccttgcggga agagagactg aagtgtgttg agcattgcgc actctaggtt ggcggcagga
     3121 tctcctgccg gggtgcgctc attgcaggta attttaacgc actgcgaagc cgcgtcttgc
     3181 gatggtctct cgacgggcca catattccgt cacgtccttt tcacgccagc ggctggtgcc
     3241 gagcacgtga atgggaggag ggaactttcc cgccgccacc cagcaggtta cggtcttggt
     3301 cgacacgccg aaagcctcgg caatctgcgc tcgcgtcaca agtttttcaa gattgagtgc
     3361 catgatcagc ctccgttttt cttttccaaa agaatgtcca tgacattccg tttcgttgcg
     3421 atgcgctcaa ggactgcctc atcgagcgtg tccttggcga cgatggtata gacaaacaca
     3481 gaccttgggt gccctgactg cgcttgtcgt gtcggcccga ttcgctccac catctggtcg
     3541 tgctcttcga gattccagtt ggtcgagtag aagacgagga tgttgccccc gtcctgcagg
     3601 ctcaggccgt gtccgcatga ggcggggtgc gcgaggagca tcgggatctt tcccgcgttc
     3661 cattcgcgga ttgtcttcgg gttcttgtcg aggacgcggg cttccttgaa ggcggctcgc
     3721 agaacctcaa gctcgtggcg gaagttgtac gcgacgagaa tcggcatgcc cgctgcttcc
     3781 tccacgatgg agcggagcgc ctccaccttc tcgctatgaa gaacgaggta gggcttggcg
     3841 tccttcgctt cggggagcat gccgtcggcg ttctcgtagt agagattgcc gcttgcgatt
     3901 tgcagacatg cgctcgtctt tgttgccgcg ttcgccgtct ccacctcctg cccgtcgatc
     3961 tcggcgtaga gcttgcgctc catgtcgtcg tagatcttgc gggcttcggc ggggagcgtg
     4021 acggagatcg tggagtagat cggctcttcg aggtcgaacc aatcctccgc gttcaccttc
     4081 atcgtcacgt cgctcaaggc ctcctgaatc ctcgtctcac tgccgggcag gatctcccac
     4141 ctcactgcga aggcggctgc gccgacgcga acggggcgga agaacttctg ctggtatgcc
     4201 gtcatcgact tgccgaggcg cttgcctgcg tccacgaacc acagctggcc ccacaggtcg
     4261 aggtagccgt tgggcgcggg cgtacccgtg agttcgataa agcggtcgac gtgcttgaaa
     4321 gcgacggtcg caagggcctt cgctctcgtg ccgccctgcc ttgtgcggaa ggacttgagc
     4381 cttgttgcct cgtccgccac gacgatcttg tagggccact tgtcgcccca gaagtccacg
     4441 agccaattga gcaggccgta gcttatggcg tagacgtccg ccttctgacg ggcgagggct
     4501 tcgcgcttgc gcttctctcc gcacaggcac acggccttca tgcctcgcag gtgatcccac
     4561 ttctccacct catcgggcca cgaggactga gcgacgcgca ccggcgcgat gacgagggca
     4621 ggcccctctc ccatcgtctg cgcgatcctg atcgccgtga gcgtggacac ggtcttcccc
     4681 atgcccatgc ctgcccatat tgctatgcgc ttgcggctca ggatcgcatc gatgatgagc
     4741 gcctgataag ggcgcggagt gaacttcctc atcagtccct cttgaagtaa agccagacgg
     4801 caacacaggt cgaggcccac atcaccgcat cccacgggaa cggaaggaag agcgcaggca
     4861 gcaacgcaac gacggggagc atcagtttcc agttatcagg gttcaccatt gcgggccctc
     4921 cctaagagca aagagacggt tggtgtcggt gcgctcacgc aggtcaagga ggattccttc
     4981 gacggcatcc gtggagtcgc agacgtagac ggggatgccg ccgttgcgca gcacctcgat
     5041 ctcccgaacc tgttcgcggc ggggcttctg ccccggggcc ttcacttcga tcatggcgaa
     5101 gcagtcgaag ccgacgatca gacggtcggg cgcaccacgc ctgccttcat aggcgagctt
     5161 gcgctggtag agtccgagct ccttgcatcg gcggacaagg aaggcaacga tttttccttc
     5221 aggtgtcact tacttctcct ttttgccgcc tgcggcgaga taaaagtccc tgccgatctg
     5281 ccagatgact ggctcgattc gactgtcaag aatcgtttcg gcgtcctctg gggtggcgcc
     5341 tttcattacc gggttgagag cgcggtagaa gcacgacatc agcgaagcga cgcctgcatc
     5401 aaggaagcgc atagcccagt gggacttccc cgcgagggca aggtagatcg cgaaggtctc
     5461 cccgacggta aaggcgggag cgcacaagtc ggtgcggttg cggatcgaat attcgagcgc
     5521 tttcccaagg gttgaaattg ccgaagccaa aaaggcaggc tggccgcgtt cctcgaagcg
     5581 gccgtcgctc cattggttgg ccgtacagcg gtgcttggat agaagcgtca gcagtccctt
     5641 ggcctcgtaa acagacatcg tgtacccgca cgcgtggagc gcaaggacgg gccaccattc
     5701 ttcgggctta aacttcgtag tcatactctt catctccgtt gtcttcgaac aagtcgcacg
     5761 ccagtgcaac ttcaaacact tcgccgtctc cgcggtggag cttcagtctg aagaagaggc
     5821 cgacctcgct cggctcccct ccgatgccga agccgaagca gggttcacgc ggaccgcgct
     5881 cggcctcgtc gacctcctcg tccgtcaggc cgtactcttt cttgagccct tcacgagtca
     5941 ttgctctaaa ctttttcact ctttcttctc cttcttttat gtgccgggcc tccgcagtca
     6001 gcggaaacgc ctcgcgagaa ctccgtgcag gggactcgaa catccgttcg gccacaggca
     6061 cagtcgcaaa ggaaggctgt cgtggtctgg ccgtcagctc tgttcacgtc gaaaccatcg
     6121 acgttcttca cgatcagcga accgatgtgc cgaccgatgt aagtgtccgc agtctcgatt
     6181 tgtctcgctc ggttcttgta cgcgagtgag ttgcggtaga acgggttgag ctttttcagc
     6241 cgctcccgtt ccagctgtgc cgcatcgaac caatgggcga atgcgttcat tcgtcttctt
     6301 cttctcccat gtatcgacgc gcagctatct gatccggatg ccaatagtca tccggcaggg
     6361 gttcgcacgg cttcggcatc ggcatccatg cgatcacgtc cccgtatttc cgaccggagc
     6421 gtgtgtccat ccatgtgctg ttttcttcga tgtagtaggc gcgatctatg aaaggctttg
     6481 tgaacagttc ggctactttg atgtttggaa tcttgattct gattccttcg ctttgtcttg
     6541 tgatgagcta cagccccctg caaggcggct tgtgcttggg aaagcgcgac cacttcgctc
     6601 gaaattcata gaccatggct tacttccccg tggagccgta gccgccggtg ccgcgagcag
     6661 tttcggaaag ctcatcagcc tcgacgaact cgacgtcttc gagcttctcg atcttgatct
     6721 gcgcaacacg atggccagcc ttcacttcga acgcgccgtc ggtgccgttc ctcacgatga
     6781 ccatgatctc gccgcggtag tcggcatcta cgatgagagg cgtgatgatg agaccgctca
     6841 cagcggagga ggagcggctg tacaccacgc cgcagtagcc cttcggaatc tccatagcga
     6901 ggcccgtcgg gatcttgacc gtctcgcctt gcgggatgac ggtgctcttc gtgcagaaga
     6961 gatcgagccc agcggcatca gcggttccac gccacgggag cttggcttcg gggtggagct
     7021 tcttaacttt gatttccatg atgaatcctc aattcggggt gacagtaaag ggcacgacgg
     7081 tagtcgtagg ttctggtgat caggtcttcg agcttgtgga gctgggagag cgttacgccc
     7141 ttgacgtctc cgaagtcgac gacgacttca ggaaacttcc ccggctcgca cttgacgacg
     7201 cggggcttga cgacgaggga ctcctcgtct ccgtgcaggg cacagactgg tatctcaatt
     7261 cgtggcttgt ggtcgtatct catttcagtg gttctgcaaa aacgatcaat gtgtatttgg
     7321 taatcgggat ctgaagttct tcattgggcg acacccactc gatgcggttc atcgtgtcat
     7381 tcttctggag gtatcccgag cgggctttcc cgttgatgta ggcgaggccc ggtcggttca
     7441 tgaagcgctt cgctctctcg tccgcaggga tcaggaacgc agacttcgga ttggctctca
     7501 gtgagaagtt gtgaaccttg atggcgtcga tgcaccgcac cagaacctct cgctccacag
     7561 ttctctgcat gtcggagatc acgccgagcg ctgcgtccac aggctcgaag agttctcgcc
     7621 atatgccctc gggttcgccg ttgcggatgc acctgcaagc gccgccaatg cctgcctcag
     7681 ccagctggat cagcatctgg atttccgtct tgttctcgaa ggcggcggca aggacgaagc
     7741 cgaaggtgag ggccgtctgg acattcgaga cgttctcttc cgtgccttcg cctcgcttga
     7801 tggcgtcaag agggacgcga gcccacagct cgatgttccc gagcatggcg tcgtcgagct
     7861 ccggcagaac gcggtagagg tatgcgcctg ccgccacgcg gcgatgcttg aagggttttt
     7921 tgccgacgcg cttcttcacg atcttcttca tgccgtcgcc accttctcag taaggatgga
     7981 gctgaagatc tcttctgtct tcacgtggag gagggtgtag agcgcaacga catccttctg
     8041 gtcgagcgtc ctcacgatgt cattcagggt tccgtagagc gttccgagaa gagccgagat
     8101 gacgtagcgg cgaaggcggc ggatgaactc gaaggcatcc ttgtagcgac cgcggtcggt
     8161 cgtgatcgct tcgttaagag cgaggatgtc cacgaggcgg caatgcacgt tgtattcatc
     8221 ctccacgtgc tcgcgcttcg tcacttcctc gaagtgctcg atggccgggc ggacgaagac
     8281 gagcagggca ggctcgatgc tctccttgaa gagcgtctcg tgggcgagag cgcagacgtc
     8341 cttcgccatg cggggagtga cgccgaaggt gaagggcgtg tgtgcgagag tggtcttgtc
     8401 aaacatttgg ttaatccttc ttgtagcggt atgcctcgta gcctgcggcg gcgaggggga
     8461 gtccggtagc ccacgtgggc agttcagtca tgagagccga catcttttcc agtgagtagt
     8521 cagcagtgtc gggtgcctcg gtgatcgctt cgtcgtgcac cgtcagcact gtcttgtatc
     8581 cggcatggtc gaggcggagc agtgcgtcgc agagcaggtc gcaggcgatt gcctgaatca
     8641 ggttctcgca gttgtgaaca atcagcgggc cttcttctcc gaggacgacg aacctgtggc
     8701 gggggccgca gttgacgagg tcgtaaacct gttcgtcacg ttgggcttgt cgaacaagtg
     8761 ctccctcggg catccgtgcg tcagtcggta gtggagagtt gtcaagccaa tcccggtttc
     8821 ccgtgcccag tccgcaagcg gcttcccgtc tatgagcacc gtccttcgct ggttgttgca
     8881 gttttcgcga gcggttaccc aacggcagtt gtccttcgag taacccttct cgttgtcgat
     8941 tcggtcgagc tgtagccctt tcttgtaggt cggtgccatg tcctcgtaga acttctcgaa
     9001 tgattcgctc cactctttgc agacagtgat tccgcgcccc ccgtagttct tccaagcttg
     9061 cgcggtcgga atctcgcagc gctctttcat ggagtgccac acgttccaga gcggtgtccc
     9121 gctcatgccg tgccgagaga tcttttcccc tatggacttc caccttctgc atccgcatga
     9181 ggcgaggttc ttgtcccttc tgtagtgccc gaggcgactg gagttcagaa caaccgtctt
     9241 cccgcaggca gagcacaggc acttccatag cgtgtggtgc ccgtccgagc ctgcatattc
     9301 cgtaaccgtc aggaatccga cagtcagccc tactaaatcc tttactctgt gatgcacagc
     9361 accaaccctc cgtagttaaa accttgtgat ccggcgtcat gcggacgccg taagccgtca
     9421 ttgtttgttt cgttcctttg tcgattgcgc cttcctgatg cacccactca acgccatccc
     9481 acacttcgtc tagtggggtg acgtcttcga tgggcgtcca acccctgcgg cagagaacgg
     9541 gtgtcccctt ggcgatgcaa gccttcgctc cgtatgattc gatgcgaacc cacttgcgcg
     9601 acatctggtt gatgcctagg taggtgaagc tgtccgcctt gccgatgtcc gacggggcgg
     9661 ggctcgggta gcagagcttg cggccggagg gaagctcgac gaggaggaag cctgccgtcc
     9721 gactcaccgt ggccggtccc acagtcagca cgagcttcgg gtcttcaagc accttcatca
     9781 cagcgccacc gagatcgccc cagagcgcac agatcttcgg gttggtcgct cgccacgctc
     9841 tcttcagcgc gtcgcaggcg atgaacacgt cgcgatgcag gccgcccgtc aggttcttct
     9901 tgaggaaata ctcgtaggag tcctcggctt cgcgccacgt catcggcgag atggtctcgc
     9961 ggacgttctt cgccatgtcg tgaaggtcga tgccgtaagc cttggcgaag cgggcgaagg
    10021 cgggagcgcc gccgccgtag ccaagagcca gttcaagcac cttgccgacc tgtcgctgct
    10081 tcttcgacac gtcctcgggc ttgatgccga aggcgcggct gtaggccagc ttgtaaaggt
    10141 cgtgtccgtg gccttcgtcg aagtcgcgga aggcttggat cttccactct tcacctgcgg
    10201 cccatgcaag aacgcggcct tccacgttcg agtagtcggc gacgacgagc ttcttgccgg
    10261 cgggagcgat gatctcactg cgcaggcagt tggtgagcac ctccatcggg tcgtcgtaga
    10321 gagcggtaag cgtccccgcc ttggcggcat cgatgcagaa gtcgatctcg tcctgcttca
    10381 gggtcgggcg agggaggttc tgcggctgaa agagacgtcc cgagaagcgc cccgtgcggg
    10441 atgcaccacg gaactgaagg catccgcgaa ggcgtccgtc cctgcccacg gcttcggcga
    10501 gcatggcgaa cttctgcacg gaggtcttcg tcgactggag acgcagacgg agaagctcgc
    10561 gcatcggctc ggggatcgtg tcgtcgttga tgcgcttctc gacctcggcc ttcgtcatcg
    10621 tcttgagcgt cacgccgtac tcgctctcga agtaggagag gagcgcgtcg cgctgagtcg
    10681 cggcttcaac cgcgccgtca gtctgttcgc gggtgcggcg ggcgaggagg actcgcgtct
    10741 cttcagcgat ggcgacggcg gcattggcca actccacatc catcagcatg ccgcgacggt
    10801 tgatctcggc atcgaggatc tgcaggtcgc gctcgtgctt gctgaggttg aacttcggca
    10861 tcttcttgaa gagtgctcgc tcggcttcga cgtcgaggcg gcagtattca acgaagtgcg
    10921 cccagtcctc ggggtgcgtg gtgcggtcgg cacggtggta cgtagagttc ttggggcgcg
    10981 gcttgcagaa gagctggacc agacgcttgc cgtccttgtc cttcgccacg tcctcagaga
    11041 gtccgaagac ggcggagagg tctccgagcg cacccggaag agagtgctgg tacgccatta
    11101 ccatggtgtc gacgatcatg cggagcggga tgtcgtagcc gtgggccttg agcaccacgg
    11161 tgtcgaagtt catcccgttg tgccacaccg tgtagcactt gcccgccatt gccgcgtcga
    11221 gaatcttctt gagcttcgcc ggcatgggcg caccgctcgt cagatcccac acttcggcgg
    11281 gctcgtcgtt gagggcgtaa gcccacagca tgatctcgca gtcctgagcg tagcggtgag
    11341 agccgaactt gatcggcgtc tcgctgtagg actctagatc gtgaaaactg tagttggcca
    11401 tcattcaatc tcccacttgc agttgagcgg gtgccacggc ttcgcgtcct cgcgctttac
    11461 gagaaccttg ccgcgggcgt aaccgttgcg gatgccccag aggcagaagg actgggagtt
    11521 ttcacgccat gccggacagc agtcggcctt gtcgagaagc tcccatagct gatgaaggcg
    11581 ggcgagcgtc agctcgatgc cgtcgccgat gcacacgccg cggtcgacct tgacgtagat
    11641 cgaggctcgc tgcgcctgct tggcgagagc ggcggtcttg gcctcggcct tcttcactgc
    11701 ggcggacttc tctgccttct tgttgactcg cttcatagtc accttcgcct tggcttcctt
    11761 cttccgcgct tcggcttttg ccttggcttc agccttggcc ttcgccttgg cttcgcgctc
    11821 ttcgcggcgc ttggcgcgag ccgtgccttc catgcggtct agttcacgag agcggttcca
    11881 gttgctgatg cggttctgac agccgcagga ggggttcgcc ttgtagcgga tgttgtcgaa
    11941 gaagtcctcg aacacatgcc cgcagtagcc gcacgtggcg gtcacgatcc agcgtgcacg
    12001 gtggctcttc gtcaccttgt cgtcttcccg aaggaagttg ccgacggtga cgtgcccgta
    12061 cttcttgccg gagaacagcg cctccatctt ttcgcgtgaa aacttttcag ccatttgaga
    12121 ctccataaaa acttgaaggg cccttagggg aggcagggtt gaggagccgt gaacatgatg
    12181 gctcgtcacc tgcagatgcc ctgcctttgg gatctgaggt aaaggcgggt aatgacaacc
    12241 cacagcctca gaccgatccg tatctgcgtc agatcgtttg cccccgcggc cctgactcaa
    12301 gccctcaccc ctcgcgctca gggggaaagg ctttatgtca gggtcgcggc atcaggccca
    12361 cggatcgtcg gacacagccg cttccttgtc gtcggtctcg agcacctcga agccttcggc
    12421 ggacgcggcg gaagcaccgc cgagcgcctc gccgtcacgg acaaactgga tgcccagaag
    12481 cgtcgcggag aagccgcggg aaccgttgtt ctcgtaggcc cacacgtcga tgcgcatgtt
    12541 gatccagcag ccgccgtagg ggatgccctg agacggggcg atggcacgga gacgctggtc
    12601 gaccacagcc ggagcgccgt ccgtctcgcg gcggcgggcc gtgaccttca tgaggccgag
    12661 gtcggcgtcc ttctgaatga ggcgcgtgtt cttgttttca agcgcttcct cgtagtaacg
    12721 ctggcccttg gcaccccatt tctggtcggc gacggccttc atgacgtcga gaatggcctt
    12781 gtgcacggca ccattggctt cgaagaggcc ggtgcattcg tacttcttgg tgccgtcttt
    12841 ggcgaaggcg ttttctcgcg cctcaaacac atgcggatag gcgcagcgca cggagatgtt
    12901 cagcttcatt tgttttcctc aatgactttg aagccctcgc ccatatccaa agcgggacga
    12961 ggatcgcttt cgggcacaac cgcgccctta ctctcattgc gcgtgatgag cgcttcgagc
    13021 tttgcccact gccgctcggt gaggacaggg gcttcacccg ccttggcctt cgtgagcttc
    13081 gcggcggcgg tcggcgagat gagtttcttt tcgtagcgaa gggcctcagg caccttcatg
    13141 gacttgagct tcgcctcggc ctccttggcg tccgcccagc ggcggatgcc tgcgcggcct
    13201 tggacgagct tgtagccggg gatcttgttt ccgatctcaa gctgacgcag ggcctccgcc
    13261 ctcacagcgc ttgtccactg gtcgataagg tcgaggacgt tcagtgcacg ggagagggcc
    13321 tcggcttcgg tcggcacggg gatcgacagg gcttcaacca cctgcggagc cgcatcgacg
    13381 atggcaggct cggccttgaa ggcttcgacc acggcgccgc ggatggcggg gcaggtgccc
    13441 ttggctcggc agaagcggca gacggactct gacggaccga agtcggcgga ctcagtcgcc
    13501 gcgccgtcac agagtgcgag agcgcgagcg cctcgggcct tgacgtcctc ggcaaaggag
    13561 cggaggccgc ccgtggtcgt cgtccagtgg tcgatgtggt tcaggcgggg ctgcacgatg
    13621 acgatgtgaa tcgtcgtcac agggtcgtcg ccgaacgggt cgagttcgtc gcacagggcc
    13681 agcgcgtaga ttgacaactg ctcattgcaa cgggcttcga ccttcacgcc acgcccgtac
    13741 ttcaggtcca cgacgtagag gtgctcgcca acgcgtacca cgcagtcgac cgtgccgtga
    13801 gcgcccgcct cgcccgtgac gctcgagacg tcgacggggt gctcgaccat gaagtagctc
    13861 ggcttgccct cgccgatgag gtcggcgatg gtgcgggcat agacgcagac ggcggcctgc
    13921 atctcgtcgg gcgcgtcggc ccagagggtt ttgaactcct ccgtttctgc gtcggtacga
    13981 cgccgcgccg tcacagcggt accgctgaac tgcggcgttc cgaggagctt gagttccgcc
    14041 aggcggtggg cgcgggtgcc ctcctcggcg aaatcagagg actcgtcggg caggtccttc
    14101 gtcaggacca ccgaggccgg gcaggcgcgc cagcggtggc aggcgctggg ggaaaggagt
    14161 gcgtgtgtca ttgcttaacc ctccagccgc gccgtcacag cgtcggtcac cggcggcagc
    14221 tcgtctttgt ccggggcagt cgatcgagat tcgtccgttt tttccttttc tgtctgcgtt
    14281 tcaggcgttt tttgttctgt ctttgccggc gtctgagcgc tttcagcggc ggcgggcgcc
    14341 tgatctgcgg ccacagtgtc agagacaggg gcggcggcct cagactggac cggggcggcc
    14401 tcagcagtgg gggcggcggc ttcaatcggg gcgggattga tcggggcggg ggccgctacg
    14461 ggggccgcgg cggcggcctc aacggtgaaa gcggcgggcg ctttcatgcc ggcggcaatg
    14521 gtgagacgca gcgctttaat ttctgcagtc aaatcagaaa gaatattttc cagtggcata
    14581 agatttgctc caaagagttg agaggaaaac gaggcggcta ttccagagaa aagccgtaga
    14641 ggccagcgag gccgtgaatc atcgaaaacg cggccggcgg cagttcgatg taatcaccgc
    14701 cggcatcatg cccgcggcgg tacggaacgc cgaagtaatc cagataggcg caccggcccg
    14761 caagaccggc agcagggccg ccgggcgtgc gaaagcgcag gcgcaccggg cgcacgcttt
    14821 tccatcgata gaaccactgg agagcggcgg cggcccgtgc cgaaaggcgg ccccgcgcgg
    14881 tgaagggcgg gcgggaaagt ggtttgatgt cgaccataga gaagggctcc aaaacggccg
    14941 cctgcgcgcg gcaggacggc cttaaagggg gttagttcag ttggaatcgt tcagggcatc
    15001 gagccgctgt atctagcgtc agatagaggg cctgcagcag gtgaaagcgc tgccgcaagg
    15061 cgcgctgggc ggcatgcgcc gccccggcgc tggagaagcg ggccggcagc acctgccagc
    15121 gggcgccggg gtaggcgcgt tgcttgattg caaggacgta aagcactcgc tcctcctttc
    15181 agttagtcgg ccatgatcgc ggcggcggtg cgctcatacg acgggcggcg gcgatcgtca
    15241 agcctgatgc ccatgacaat ggcggcggcg tcatcggcca gggcaaaaac ggcctgcatc
    15301 tgattcacga tttcggccga aaggtcggcg cgctcacggg cggggcgaag cgcggcggcg
    15361 gccttagtaa cggcagtcaa atacttgcaa ctcacgacgg ggatcggggc ggcgggatcg
    15421 gtatacgtag gaatgatgcg ccgatagtcg acatacttcg tatcaacgag cgagataggc
    15481 gcagaaaacg cgctagagcc cgtgcggtcg agcctgaaat tgaaaacgcg caggcgcccg
    15541 gagcggcggt cttcgtctat ctcaatttcg agagtgtccg cattcttgac ggccgtttta
    15601 acggccttca gggccgcggc cagcggggcg cgatgaatgg tgacgggctc gaaaggctgc
    15661 agcgcgccgg cagtgtcgac cacggacggg gcggcctctg cgcggccgat gaaaagcgcg
    15721 ctggcgtcag tagcgaccac atgggcggcg ccgccttgag gcgcgatatg caccgcattt
    15781 aaaaagcatt taatggagtc tttcggcgcg aattcgagcg cggcggaaag gagataataa
    15841 gaccgaatag aaagagaaat aaccgtcatt tttaaagcct ttttaatttt gtttgagggg
    15901 gccgcccgtt ttatggggcg gccggggcgg gcgggttagt tcagaaaaaa caccacatca
    15961 gtcgcatagc agtcggcggg cagtccgaag gccggggcgc tgcagaagtg cgggcattct
    16021 tcggacggcc acgagaagca gtacgaacta gcgtgaaggc gggcgcggtg ttcctctagt
    16081 tcagactcaa agcgctcgat ggcctcgagg tcctcggggt taagcccggt gccgtcgctg
    16141 ttctcgagcg ggcagaccgc ccaggcgggg aggttgaaag agaggatttc ggtacgcata
    16201 tcggcccccc ttattcagcg aaatcggcag agttggaaag gtaggcgcgc agcgggcaat
    16261 cgccttcaaa gccgtagagc tcaatgaacg cgctgcccca gtgggcctca acggtgatcg
    16321 aaccgtagcg agactcatag cgggcggtta ccgtggggcc gccgcaggcg aggaccgcat
    16381 caatgatgcg attgccgttg tcggcggtga acacttccac ggaaagcggt tcattgtggt
    16441 attcgcccgt ctcgtcgtcg taggcgccgc agtcaacggc gcccacggcc tgcagtacga
    16501 cctctgcagc ctcatcggcg ctgtgccagc gggcggcgcg gacatagaag ccgccggaga
    16561 gtttgaaagc ttcattgatt gccgagagga cggccgggcg cgctgcgaac agttcgcgaa
    16621 gatcagagac cgagggggcg cgggtggtgt tgaattccgt catttggttt gcctttatta
    16681 gttagttgag agattcagcg gcgaaatgcg ccgccagtgc gagggccaga aggcccagaa
    16741 aaacgagagt gccgggccag acgggccagt acttgacgcc gtagcaggca tagagcgcta
    16801 ggccgtagca ggcgcaacga agagagaagc gggcggcggt catcatcggc actccttgca
    16861 ggtgaggcac tggcggtagc tgcgaccgtc cagtgcgttg tggccggcta tatagatttc
    16921 gcgggaggcc ttgaaggctt cgccgtagct gctgaacgaa agggcggggc ggcggccttc
    16981 actgccggag aggcggcgcg ctacgttgat cttgaggaga attgtcattt gtttgccttt
    17041 attggtagtt ggaactgtac tagataactg ccgttatctg gtacacatca aatatagcac
    17101 accttgcgct agctgtacta tgtagagtgc gacaactgtt acagcttgtt gacttgcatc
    17161 aatccgccgg cagcggggcg gcttatataa agaaaggcgg cagggccggc agtggtgagg
    17221 cgggccggcg ggcggcgctt tccaccagtg agagggcggg gcaggggcgg cgccgccggt
    17281 tctaccggtg ggaggggctt atgagggcag gaaagaggcg gaagggttaa cccttagagt
    17341 caaaacgcct ttttaagggt gtataggggt aactacctat ttcggctaac aaaaaaacta
    17401 ccgaactact atttacaacc ttttttctta aagtccgtgt gaaaagaata tataagtaat
    17461 aaccctataa ggaaaacccc tatatagatt ctctatagga agtttgagga atttgcttgc
    17521 attcggtagt tcggtagttt tccgcttttt ctgggcggcc catggatgaa aggcggcggc
    17581 aatggtgaga ggcggcagaa tctgcgcagc aattaaatgc gcgcttgatt tgcggcgggc
    17641 gggcctgtag tgatgaggcg ccggcataga gtggagacgc cggcagagtg aaggcaatgg
    17701 acgggcggca gtggagccac tggacgggcg gcgggctttt cggttgtctg aacattgagc
    17761 cgcgaatggc acgcgctttg ctcggcggcc tgcgcgtgcg tttcagtttg ggggaatagc
    17821 ggggcgccca ttacccgccc cgcgggcccg gtctatgccg ccagtgtgtg gggtgaatgt
    17881 atgaatatgt ttgctccccc ctctccactc attgcgcggc gcgggtagcg gcgcgcctat
    17941 tcggcgcgta gcgcaggggc gcagcaggcc cgcggcctgc gaagcggcgg cggcatgatg
    18001 ccggcagagg cagggccgcg ccttttggca caccttccgc cccggttgaa ccctgcccgc
    18061 cccgccgccc gcccgccttg agcaaaaccc ccatcggtac cattttcgcc cgataccggt
    18121 cgaccccccg ggggccaaaa atttgcccgc ccccgcaggg gaaggtggcg tccgtagtgg
    18181 ggcaattttg aaaaccattt tggacttgtc cttcactgtc cctatttgtc ctctcgcccc
    18241 cgaacgcccg tctccgaacc ctcatccctc aactctccga aggaggccat gccatgacgc
    18301 gagccgccaa gaagcccgtc gaaaaaattt ctgagaaaaa aactggaacg aatcctgagt
    18361 gtccgtcaaa gtccccgaaa gtccgtacac ccgcgagaag gccgaaaaca gagcctgtgg
    18421 cgcgtctcga acctgcggat gaacaggtac accaactcaa gcccatgacc gtcatagcca
    18481 cgccaattgc cccctcagac gacgcgatgg cgccacggct gaggattgag cgcgacggtg
    18541 agtacgagat tccgtcgaag ctcaatcccg cgctgaggac tcggaagtac aaggagccgc
    18601 accccgactc cgagtggagg ccgtggtgga agccgcgcaa gcgtcccttg ggcggagcga
    18661 agaatccgat ggtgaagaag attcgcgagc agatcgagaa ggagcttgcg atggacgagg
    18721 gcgagctggc ccgcattcct cgcgatgcgg agacgggcag ggtggtgaag tcctatgagc
    18781 ttttcacggc tttgctctac tgggtggcgc gaggcggcac tcttcgctcg ttctgcattg
    18841 ccgttgacct gccttacggc acgtgctgcg gatggctgga gaaggagccc gaggtgaagg
    18901 cgcagtacaa ggaggcggag cggatctcgg cggatgcgct cgtggagtat gcgcaggagc
    18961 tggcgacgaa cccgtacatc gtggaggagg tcttcgagag ctacgacggg gaggggaatc
    19021 tgatccgcaa ggacgttcgc cgtcaggatg cggtgtatgc gaggaagctg tgcgtgaact
    19081 tctgccagtg gcttgtcgcc aagcgggcgc cagagaagta cggcgagaag ctgaaggtgg
    19141 agaccgacgt ctccatggcg ggcgccatcg tagcggcgcg ccgccgtctt gcggcccgcg
    19201 acgacgacat cgacgctgag gacatcgagt aaaaaagttg cccgctttag cggcgggctg
    19261 tagcatgaaa taaacctttt gggagctaaa aaatgggaac tgttagggat agaaacacga
    19321 tgctttccgc gccggagatc gagcttttga agatgacgca ggaagtcatc aagcgcatgg
    19381 cggacaactc ggccaaaacg aagacgttgt ttgtcggtgt cacgactgcc gccattgcgt
    19441 ttttgaaatt cgatccgagc gtccacaacc tgctggcgct tctggcctat ttggtcgtca
    19501 ctgtcgccct ctgggagacg gacgctcggt accttcgcct ggagcggctc ttccggcatc
    19561 agcaccatgc gatcgtggat ggatccgtcc ccgcgcttga gctgtggcgc atgaacctct
    19621 cgcgctatga ggaggagtcg gtaggcagga tcatgttcac caacttcaca atgtggatct
    19681 atcagatcgc ggtgcttgtc tccgccttgc ttctagcgaa ggtaggcggc tctttggtag
    19741 gcgtcttcta gccacttgcc gatccagaaa cgacctgacg gtagccagct gtaccgcttg
    19801 atctcgtaat cataggggtc cagaagtcca cggt
//
LOCUS       DBMDTI010000013        19514 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_937_length_19514_cov_3.891619, whole genome shotgun sequence.
ACCESSION   DBMDTI010000013 DBMDTI010000000
VERSION     DBMDTI010000013.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 19514)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19514)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19514
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_937_length_19514_cov_3.891619"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            211..714
                     /gene="ribH"
                     /locus_tag="ACZPB6_01710"
     CDS             211..714
                     /gene="ribH"
                     /locus_tag="ACZPB6_01710"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR1183227.1"
                     /translation="MAVDIVPTKLDGTDLRIGIVVARFNEYAGRGEFESCMDELRKLG
                     VMEEDVTKVSVPGALEIPFALQKLAQTGEYDALIALGAVIRGETYHFELVSNESGSGI
                     TRVCLDYDIPIANGVLTTENDEQCEERIDMKGRDCARCAVEMANFAKEFCVDEFDEDF
                     DEVTSED"
     gene            741..1250
                     /gene="nusB"
                     /locus_tag="ACZPB6_01715"
     CDS             741..1250
                     /gene="nusB"
                     /locus_tag="ACZPB6_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR1183228.1"
                     /translation="MTEHKEGHRKSAAHSARHLARVFVMLGLYQWLADPSQDYAGIEA
                     HLSSLLHDEEEQLEGSDIRPTDFEKADRELFTKLLAGVLDRHAEVADAIARHADRELS
                     RISLVERAVLMIGTYELMACPETPWRVILNESIELVKQFGSGYRFTNAVLELVAREVR
                     PEETAAPRA"
     gene            1347..2342
                     /gene="thiL"
                     /locus_tag="ACZPB6_01720"
     CDS             1347..2342
                     /gene="thiL"
                     /locus_tag="ACZPB6_01720"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR1183229.1"
                     /translation="MRRGEFQIIDRFFTHDAFGEWRSQGVGDDCAIIDMPGNRIAVTT
                     DMMAVGTHFLPGARPEDIGYKALAVNLSDLAAAGASPRAFFLSIGLPERDDEWLAGFS
                     RGLMALAESSGCALLGGDTTRTAEVGGTHAPVTISITAMGSLPEGKGLTRAGAKPGDD
                     IWVSGTVGDAYAALKHRWGHWQLMPDAAEALFPRMDRPTPRNMLGSALLDLATACADV
                     SDGLLADLGHILERSGVSGSILWEAVPVSSALRALSPAAQQEAALTGGDDYELVFTAP
                     PSAALRIEQAGLLTKTRLTRIGKICEKVSGDNLTVRSQNGGIVTTPTQGFDHFAA"
     gene            2418..2936
                     /locus_tag="ACZPB6_01725"
     CDS             2418..2936
                     /locus_tag="ACZPB6_01725"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR1183230.1"
                     /translation="MLQKYDDSNPANQIELTSRFVWSHPAHMIATFFGAGLPSKAPGT
                     YGSLAGALSFIVLSPAMTTAAWVILSIALFIAGAWAAGKVAADLGVEDHKGVVVDEVV
                     GIWLLFAFLPAGTLWWVAGFAAFRFFDIMKLPPVDIIDEKMKNGAGVMLDDVVAAFYA
                     WVVVSILGWLFG"
     gene            2942..3481
                     /locus_tag="ACZPB6_01730"
     CDS             2942..3481
                     /locus_tag="ACZPB6_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR1183231.1"
                     /translation="MPDLNERIALAAERLGVVAAERRHVVVTAESCTGGGIAAAITEV
                     PGSSAWFREGFVTYAAEAKTSLLDVDPKMIEEEGVVSEDVAQAMARGALARCPDSTLA
                     VAVTGVAGPTGGTHLTPVGTVCIGWAERFNEHIVTYVRTIHAHGSRSMVRRATVLTAL
                     EGLIELMRFGNPAVMPCEY"
     gene            3564..4886
                     /locus_tag="ACZPB6_01735"
     CDS             3564..4886
                     /locus_tag="ACZPB6_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR1183232.1"
                     /translation="MSSWFDILILVILIGMNGAFALSEVALITSRRARLQHMDEESVP
                     GARRAMDMNADPNRALSTIQVGITSIGLLSGIFGESALAKPVAEWLVSLGMGEETASA
                     VGITLVVVLLTYFSIVMGELVPKRIGQMAPERLACRVSPVLHMLSLAAAPFVKLLSLS
                     TASILRLMRVDTRSQNAVTEEEIHAMIDEGEESGVIETVERDMVRNVFRLDDRQVASL
                     MTPRADIDWIDLEDSPEVNIEKIRSSKRSRLPVCVGSLDDVKGFCSTRSLLQQMMEKG
                     RADFKDNLVAPVYVPESLTGMELLENFRKTDTPVALVVDEYGEVQGIVTPRDVLEAIA
                     GEFKPEQPGDAWVTRREDGSLLLDGMIPIPELKDVLDIKSVPEEDFGRYNTLAGMMML
                     LLGRLPKEGDVAEWDGWRLEIIDMDRRRIDKVLAVKVKTAPKQEGRGE"
     gene            4911..6086
                     /locus_tag="ACZPB6_01740"
     CDS             4911..6086
                     /locus_tag="ACZPB6_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183233.1"
                     /translation="MAVKKAWVACGAAVVLAAVASGGVWLQGRVFDRQFDEFARELAD
                     TSVDMGFLLSAEQTGRGFAHRDLAIKLRGKDFRLCWTGRAEFGLGMTARLTLDKEYGS
                     MANLPEYGIEGLDDELVLKTGLFSSKPELAWRVKPFRVAAGSLLCSAGGLELTSGDVE
                     HEGRLVWRGLSCSEGGESLRTGDVTFDVSVSKDGRRAEMKAMFEGGSADVEGMNLKTG
                     AFEMASTIEETRAETQEDDALYSQSWTLKSGELDYDGVRVLDALTFRANMTNVPERLV
                     DAMALGGAVSPVILEMAAEQAIMQGGMTFDLDECTVTRGEGTLSAAGRVARDGERIGA
                     FSVRLDPEFGADVKGWPEAVAELKANGSLRTVEGRLEAHVELSTDGVAVNGVKLDSL"
     gene            complement(6161..6973)
                     /gene="pyrF"
                     /locus_tag="ACZPB6_01745"
     CDS             complement(6161..6973)
                     /gene="pyrF"
                     /locus_tag="ACZPB6_01745"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR1183234.1"
                     /translation="MKFTEMLSERWQNAGSMLCVGLDPNPTRFPHELAGRSTALYDFC
                     CGIIDATADLVCAFKPQIAYFASCGAEAELKAVIDYIHANHPKVPVILDAKRGDIGST
                     AEHYAREAFERYEADCVTLSPFMGFDTIKPYLAYENKGAFILCRTSNPGGDDIQMLEV
                     NGEKIYERLARLAATEWNLNGELGLVVGATYPAELANVRRIAPNLPLLVPGIGAQGGD
                     IRACVEAGKTAEGTGMVINSGRAIIYASREDDWKEAARKTAIATRDSIQAAV"
     gene            7127..7717
                     /locus_tag="ACZPB6_01750"
     CDS             7127..7717
                     /locus_tag="ACZPB6_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183235.1"
                     /translation="MITRRQFIGAAGLMTVAATAPAFAYESRWPKKPSVPTDLNAEEF
                     FTELAEKGSGFVVGNEKADTVVMMTFDTQCRWCVWEYEQFKPFLDRVKFVWHPVAVLN
                     PWSELQGAAILAAKDPKAMFLEHEAHMKDAVFKGLDVRKMEIPFEKREAVWTNTKVFR
                     RAAAREVPFGVMKKPDGSYVAVCEQTGESFAKLLGK"
     gene            complement(7841..9478)
                     /gene="groL"
                     /locus_tag="ACZPB6_01755"
     CDS             complement(7841..9478)
                     /gene="groL"
                     /locus_tag="ACZPB6_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238928.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR1183236.1"
                     /translation="MAAKQVLFGDDARSKMVRGINVLANAVKVTLGPKGRNVVLDRAF
                     GAPTVTKDGVSVAKEIELKDKFENMGACMVREVASKTSDNAGDGTTTATVLAQAIVDE
                     GMKFVAAGMNPMDLKRGIDKAVAAAIEELRKISKPTTTNKEIAQVGAISANSDAEIGD
                     IISEAMDKVGKEGVITVEDGKSLKNELEVVEGMQFDRGYLSPYFINQPEKQAAVLDNP
                     FILLHDKKISNIRELLPVLEQVAKAARPLVIIAEDIDGEALATLVVNSIRGILKVVAV
                     KAPGFGDRRAAMLEDIAVLTGGTVISTNVGLTLDKAALEQLGTAKKVEVTKENTTIID
                     GAGQAEAIENRVRNIRTQIEAATSDYDREKLQERVAKLAGGVALIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALIRAKQAIRDIKGDNDEQNAGIRIVLRAMEEP
                     LRQIVANAGDEPSVVVNTVAQGEGNFGYNAQTGEYGDLVEMGVLDPTKVTRTALQNAA
                     SVASLILTTDCMVADIPQDDKPMPAGMEGMGGMPGMM"
     gene            complement(9540..9827)
                     /locus_tag="ACZPB6_01760"
     CDS             complement(9540..9827)
                     /locus_tag="ACZPB6_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR1183237.1"
                     /translation="MQIRPLHDRVIIKRLAAETTTSFGIVIPDSAGEKPDQGEVIAVG
                     PGKRDEAGRIIALDVKVGDRVLFGKYSGQTVKVDGEELLVMREEDIMGVLA"
     gene            10186..11622
                     /locus_tag="ACZPB6_01765"
     CDS             10186..11622
                     /locus_tag="ACZPB6_01765"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HIR1183238.1"
                     /translation="MLRKAAADCGVDPRAVTVSVVALDGLRMPEGGRMPVVRPLLAVN
                     ADRSVAPASTAKLVTTLAGLELLGADWKWRTGFYAKARPDAEGRVNGLWLRGGGDPTL
                     VAEKFGLLVERMAQMGVRRIEGDLTVDRSYFDLPEGDPSAFDGRGSRPYNQLPDAAVA
                     GYRSLSFEFVPDESSGTARIISMPPLSGLEVPSTIRLSRGACGDWKSTIGYRLEHLSD
                     GRLAARFEGSLPLSCGPKTFSVVSLSQNEYLERLFRWYWERDGRTWTGHVAEGRVPEG
                     ALKLAERESDALPVVTTLVNKWSNNLIARHIFLTLGTLRNAPDEADSGSRTAGGAFAP
                     MERPRPGVDTDDARAVLAGWLAEKGVPDGAVMIDNGSGLSRTSRVTARAMTQILAAGW
                     LSPRMTEYAASLPVSGVDGTMRKRLEATGRTHLKTGYLQDVRSIGGYVYAKNGRRYAL
                     YASVHGERNMAGGIRFLDKVVEWTYLQK"
     gene            complement(11740..13890)
                     /locus_tag="ACZPB6_01770"
     CDS             complement(11740..13890)
                     /locus_tag="ACZPB6_01770"
                     /EC_number="1.8.-.-"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HIR1183239.1"
                     /translation="MGSVKTWLAAAALCAAAASTWASGAQDLLSTLDRAAQPAPEPSV
                     TVRLVAQNEGLTPQAENLLAVVLEHAPGWHTYWRMPGDAGLTTQFSFTTPKDVRLTDP
                     RFPLPERHNDKGIVSFSYGGTAVFPFETEIPRFPEGRSARISIHVSYLACKDVCIPGE
                     GTAEITLPYEVAPKPSADAELVSNAIVNVPEVADIRGVRAVYEEDRLKVTVPASEVHV
                     EKSLSFYPLDERVLKLSDEPQHQTEEDGSASILMTLAPEFLQEPRATLSGVFVGDGGP
                     AAGGWAIETSFPLEKGAVPKPAIFSGALPVAGSGASVTTLTAIVFAFLGGLILNLMPC
                     VFPILSLKILQLIEGYRRGERLLPHGIAFTGGVLVTMGVLAGALLALRGVGMALGWGF
                     QLQSPWVVSLLLVLFVAITINLLGVFEFTAASHLADTRVARSAPRTGAASSFFTGILA
                     VIVASPCTAPFMGAALGYAVTQPAVEAIAVFLSLGLGMALPWLLLCIFPGWSRMLPRP
                     GAWMDVFRKVMAIPMALAVVWLGWVLSKQVSLNGMLLMLAGCGSAAIALWLLGREQWG
                     RGRNRVLMGVTSFLAAGAIAVIATGEFDRAATAQGRGVWQPWSEEAVRASLAEGRPVF
                     VDFTAAWCVTCQANKVAALDRDEVQTAFRDGNVTLLMGDWTNRDPAITDILSRFGRSG
                     VPLYLLYRPDGTVQVLPELLTPGIVLDAVKGGQQ"
     gene            complement(13915..15198)
                     /gene="hemL"
                     /locus_tag="ACZPB6_01775"
     CDS             complement(13915..15198)
                     /gene="hemL"
                     /locus_tag="ACZPB6_01775"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR1183240.1"
                     /translation="MDLNDELFQRAQISIPGGVNSPVRAFRSVGGSPRFIDRAKGPYM
                     WDATGKQFIDYILSWGPMILGHNNDEVIAAVDEAVSKGLSFGAVTQGETLIAEEVRKL
                     VPSMDQVRLVSSGTEAGMSAIRLARGYTGRNKIIKFEGCYHGHSDSLLVKAGSGMLTF
                     GNPSSAGVPASVTEHTLVLEYNNPQQLEDAFAQWGDDIACVIVEAVAGNMNMVRGNPE
                     FLRTMRELCTKHGAVLIVDEVMTGFRVAQGGAQAFYGIEPDLTMLGKVIGGGMPVAAF
                     GGRREIMQQIAPLGPVYQAGTLSGNPVAVACGLATLKGIQAPGFQDKLSRAADRLVKG
                     LTAAAQENGITFCADSVGGMFGLYFLPSVPHGFADVMKSDTETFNRFFHGMLERGVYF
                     APSVFEAGFVSIMHTDEVIDATIAAARETFAEIAA"
     gene            complement(15383..16537)
                     /locus_tag="ACZPB6_01780"
     CDS             complement(15383..16537)
                     /locus_tag="ACZPB6_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR1183241.1"
                     /translation="MNIKMKTLAAATAAAACAAGQAHAGFLEAVIDTTPAGYVSSSAE
                     RPGDFGSFWQDTVRGAKAIMKEGNSLWVVPTYTNHPTWDWDKRHEENGYPFGMGLGRQ
                     VIDNRGNERSFFLVTFVDSNYRPEPVAGYQWVARWPVAGTGLHVGAGYLAAVSARGDY
                     MWVPFPMPLPVAKIGTDDISFYGTYIPITNVFFFYSTITIDDAKRRDLPLPATSAWSA
                     DRNYLYGGWGWEYMDNGEEFSPSNVKNDSSWHVGMRHYSGRHWATDVSYRRSEHDIKT
                     AGTDTSKSYRFQTWAVQLQYNIDALDSLRLYAGGGLGYSKMKGPAGKDDSFHPVTSLG
                     ATYAVTKNVFVNAEMFTSFARYKGTVEARGDDYVLKPMATDFTLSLGVAF"
     gene            16743..17050
                     /gene="rnpB"
                     /locus_tag="ACZPB6_01785"
     ncRNA           16743..17050
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACZPB6_01785"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(17122..19251)
                     /locus_tag="ACZPB6_01790"
     CDS             complement(17122..19251)
                     /locus_tag="ACZPB6_01790"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR1183242.1"
                     /translation="MNILNKIMKPRSIAVVGASTKQHTIGSDIMKRLREYGYAGPVYP
                     VNPKGGVIEGLPAYATVLDIPGEVDLAVIIVNARFVLQTIDQCHEKGIKGLCVISAGF
                     KETGTEGARLEAELLERVRRYGMRCVGPNCLGVVNTHPAVRMDACFAEALPERGNIGF
                     VSQSGALGGGILNILRDLNLGFAQFISIGNQADVNAETALEYWEGDEDVRQILLYMES
                     IQNPANFRRIASRLSKKKPVLALKAGRSAAGASAASSHTGSLAGADLAADALLRQSGV
                     IREFSLKNLFATAKVFANCPIPKGDRVAIVTNSGGPGIMGTDAICEKGMKMAELTDET
                     KAKLRSFLPAAASVRNPVDMIASAPIEHYRQTLETVLADPNVDMVITIYLPFLGLKDT
                     DVARAIMEIKAAHPEKPVVGVFMTKSEFFTAFSAADVNVPFFMYVEEAVEGLARLNEQ
                     RLWIERPEGVLPDFKVDGERARNIIRTALADGRDQLTTRESIDVLDAYGVRVCRAGFA
                     ADEDAAVAVADRIGYPVVMKMTSKTTSHKTDVGGVRVNIGSAEALRAEYRDIVAKLDE
                     RGLLDGLEGVIIQEMVKGSREMVCGIATDPQYGPMLMFGLGGVFVEVMKDVAFRIAPV
                     TDTDARDMVRSVKAYKLLQGTRGTTPADIAKVEETLLRLSQLVTDLPFIDELDINPLM
                     ISEKTGEPIAVDGRIKVRRCEAESALA"
BASE COUNT         3411 a         5766 c         6807 g         3530 t
ORIGIN      
        1 cgttcttctg gtgaagggcg ccgtttttcg acagcggggc cgaaaaaacg gcgcccgcac
       61 ccgcccgccc tgcgtcacgc aggtctttcg cgttaaaata atcgtctatc agtcgtcgcc
      121 ggcaggccgg cggcggcccg agggcctggc cttcgggatc ttttcaaaac gaattcagct
      181 cccgcctgca gaggccccta ggagacagac atggctgttg atattgttcc caccaaactc
      241 gacggtaccg accttcgcat cggcatcgtg gtcgcccgct tcaacgaata tgccggccgc
      301 ggtgaattcg agtcctgcat ggacgaactg cgcaagctcg gcgtgatgga agaggacgtg
      361 acgaaggtgt ccgtgccggg tgccctcgaa atcccgttcg cgctgcagaa gctcgcccag
      421 acgggcgaat acgacgccct gatcgccctc ggcgccgtta tccgcggtga aacctaccac
      481 tttgagctcg tgagcaacga gtccggcagc ggcatcaccc gcgtctgtct cgactacgac
      541 attcccatcg ccaacggcgt gctgacgacg gaaaacgacg agcagtgcga ggaacgcatc
      601 gacatgaagg gccgcgactg cgcacgctgc gccgttgaaa tggccaactt cgccaaggag
      661 ttctgcgtcg acgagttcga cgaggatttc gatgaagtga cctccgaaga ctgatgccgt
      721 catcaaagga atcttgaaga atgactgaac acaaggaagg ccaccgcaag tcggcggcgc
      781 actccgcccg ccatctggcc cgcgtcttcg tgatgctcgg cctctaccag tggctcgccg
      841 atccgtccca ggactacgcc ggcatcgaag cccacctctc gagccttctg cacgacgagg
      901 aagagcagct cgaaggcagc gacatccgtc cgacggactt cgagaaggcc gatcgcgagc
      961 tcttcacgaa gctgctcgcc ggcgtgcttg accgtcatgc cgaagtggcg gacgccatcg
     1021 cccgtcacgc cgaccgcgaa ctctcccgca tttcgctcgt cgaacgtgcg gtgctcatga
     1081 tcggcacgta cgaactcatg gcctgcccgg aaacgccctg gcgcgtaatc ctcaacgaat
     1141 ccatcgagct cgtcaagcag ttcggcagcg gctaccgctt cacgaacgcc gttctcgagc
     1201 ttgtcgcgcg cgaagttcgt cccgaagaga cggctgcgcc gcgcgcctga cgaatcagtc
     1261 tctgactgat tcccacaaac gggccgttcc cttcggaggg agcggctctt tttcttggaa
     1321 gcgtgctttt tttcggaggt agaaaaatgc gacgcggaga atttcagatc atcgaccgct
     1381 tcttcacgca tgacgcgttc ggagagtggc gaagtcaggg cgtgggcgac gactgcgcca
     1441 tcatcgacat gccgggcaac cgcattgcgg tgacgacgga catgatggcc gtgggcacgc
     1501 actttctgcc cggcgccagg cccgaggaca tcggctacaa ggccctcgcc gtcaatctgt
     1561 cggaccttgc ggccgcaggc gcgtcgccgc gagcgttctt tctctcgatc ggactgccag
     1621 agcgtgacga cgaatggctt gcgggctttt cccgcggcct gatggccctt gcggagagct
     1681 cgggctgcgc gcttctcggg ggcgacacga cccgaacggc tgaggtcggc ggcacgcatg
     1741 cgcccgtgac gatctcgatc acggcgatgg gctcgcttcc cgaagggaag ggactcacgc
     1801 gcgcgggcgc gaagcccggc gacgacatct gggtctcggg tacggtgggc gacgcatatg
     1861 cggcgctcaa gcaccgctgg ggccactggc agctgatgcc cgatgcggcc gaggcgcttt
     1921 ttcccagaat ggatcgcccg acgccgagga acatgctcgg ctcggcgctt cttgatcttg
     1981 cgacggcctg cgcggacgtc tcggacggcc tgctggcgga tctggggcac attctcgaac
     2041 gcagcggcgt ctcgggcagc attctctggg aggccgtgcc cgtctcgagc gcgctcaggg
     2101 cgctctcccc cgccgcacag caggaggcgg cgctgaccgg cggcgacgac tacgagctcg
     2161 tcttcacggc ccctccctcc gccgctcttc gcatcgagca ggcgggactt ttgacaaaga
     2221 ccagacttac ccgaattggt aagatttgcg aaaaagtctc gggcgataat ctcactgtcc
     2281 gctcgcaaaa cggcggcatc gtgacgacgc ccacgcaggg gttcgaccac tttgccgcct
     2341 gaggcgaagc gccaggaccc tgatctccga cccccagcgc cgcaggccaa gcctgcggcg
     2401 gcaaccagga agtgaacatg ctgcagaagt acgacgactc caatcctgct aatcagatcg
     2461 aattgaccag ccgtttcgtc tggtcccatc cggcacacat gatcgccacg ttctttggtg
     2521 cgggtcttcc gtccaaggcg cccggcacct acggctcgct cgcgggcgcg ctctccttca
     2581 tcgtgctctc tccggccatg acgaccgccg cgtgggtgat tctctcgatc gccctcttca
     2641 tcgcgggcgc ctgggcagcg gggaaggttg cggccgatct cggcgtcgag gaccacaagg
     2701 gcgtcgtcgt cgacgaggtg gtcggcatct ggcttctctt cgcgttcctg cctgcgggca
     2761 ccctctggtg ggtcgcgggc tttgcggcct tccgcttctt cgacatcatg aagctgccgc
     2821 cggtcgacat catcgatgaa aagatgaaga acggcgcggg cgtcatgctc gacgacgtgg
     2881 tcgcggcctt ctacgcctgg gtcgtcgtca gcattctcgg ctggctcttc ggctgaggat
     2941 catgcctgat ctcaacgagc gcatagcgct ggcggccgag cgtctcggcg tcgtcgccgc
     3001 agagaggcgg cacgtcgtcg tgacggccga aagctgcacg ggcggcggca tcgccgccgc
     3061 catcaccgag gtgcccggca gctccgcatg gttccgcgag ggcttcgtga cctatgcggc
     3121 cgaagccaag acatcgcttc tcgacgtcga tccgaagatg atcgaggagg aaggcgtcgt
     3181 gagcgaggac gtggctcagg ccatggccag aggtgcgctc gcacgctgtc ccgactcgac
     3241 gcttgccgtc gccgtgacgg gcgtggcggg accgacgggc ggcacgcacc tcacgcccgt
     3301 gggcacggtg tgcatcggct gggccgaacg cttcaacgag cacatcgtca cgtacgtgcg
     3361 cacgatccat gcgcacggca gccgctcgat ggtgcgcagg gcgaccgtgc tcacggccct
     3421 cgagggcttg attgagctca tgcgtttcgg caatcccgct gtgatgccgt gcgaatattg
     3481 actacaattt gagaactgca gttctacaac gggcgcctcc tgaatcgatt cgcagggcgc
     3541 ctttttttgt ctttgcttaa atcatgtcca gttggtttga catattgatt ctggtgatcc
     3601 tcatcggcat gaacggggcc tttgcgctca gcgaggtcgc gctcatcacg agccgcaggg
     3661 cgaggcttca gcacatggac gaggagagtg ttcccggcgc gagacgcgcc atggacatga
     3721 atgcggaccc caaccgtgcg ctctcgacca ttcaggtggg catcacgtcc atcggacttc
     3781 tctcgggcat cttcggcgag tccgcgctcg ccaagcccgt ggccgaatgg ctcgtctcgc
     3841 tcggcatggg cgaggagaca gcctcggccg tcggcatcac gctcgtcgtg gtgctgctca
     3901 cgtacttctc gatcgtgatg ggcgagctcg tccccaagcg catcggccag atggcgcccg
     3961 aacgcctggc gtgccgcgtc tctcccgtcc tgcacatgct ttcgcttgcc gccgcgccgt
     4021 tcgtgaagct gctgtcgctc tccacggcct cgatcctcag gctgatgcgc gtcgacacgc
     4081 gcagccagaa cgccgtgacg gaggaggaga ttcacgcgat gatcgacgaa ggcgaggagt
     4141 cgggcgtgat cgagacggtc gagcgcgaca tggtgcgcaa cgtcttcagg ctcgacgacc
     4201 gtcaggtggc ctcgctgatg acgccgaggg ccgacatcga ctggatcgac ctcgaggact
     4261 cgcccgaggt caacatcgag aagatccgct cctcgaagcg ttcgcggctt cccgtctgcg
     4321 tcggcagtct tgacgacgtg aagggcttct gctcgacgcg ctcgcttctg cagcagatga
     4381 tggaaaaggg ccgcgccgac ttcaaggaca atctcgtggc gcccgtctac gtgcccgagt
     4441 cgctgaccgg catggagctt ctggagaact tcaggaagac cgacacgcct gttgcgcttg
     4501 tcgtcgacga gtacggcgaa gtccagggca tcgtgacgcc cagggacgtg ctcgaggcca
     4561 ttgcgggcga attcaagccc gaacagccgg gcgacgcctg ggtgacgagg cgcgaggacg
     4621 gttcgctcct gctcgacggc atgattccga tccccgaact caaggacgtg ctcgacatca
     4681 agagcgtgcc cgaggaggac tttggacgct acaacacgct cgccggcatg atgatgctcc
     4741 tgctcggacg tcttccgaag gaaggtgacg tcgccgagtg ggacggctgg cggcttgaga
     4801 tcatcgacat ggaccgccgc cgcatcgaca aggtgctcgc agtgaaggtc aagaccgcgc
     4861 cgaagcagga aggccggggc gaataaaaga agagagaaag gagaaggagg atggcagtga
     4921 agaaggcatg ggttgcgtgc ggtgcggctg tggtgctcgc cgccgttgcg tcgggaggcg
     4981 tgtggttgca gggccgcgtc ttcgacaggc agttcgacga gttcgcccgc gagctggccg
     5041 acacgagcgt ggatatgggc tttctgctga gcgcagaaca gacgggcagg ggcttcgcgc
     5101 accgtgatct ggcgatcaag ctgcgcggga aggattttcg cctgtgctgg acggggcgcg
     5161 cggagttcgg tctcggcatg acggcaaggc tgacgcttga caaggaatat ggctcaatgg
     5221 cgaatcttcc cgaatacggc atcgaggggc ttgacgacga actcgtcttg aagacggggc
     5281 tgttctcctc gaagcccgaa ctcgcctggc gcgtgaagcc cttcagggtg gccgcaggca
     5341 gtctgctctg cagcgcgggc ggccttgagc tcacgtcggg tgacgtcgag cacgaggggc
     5401 gactcgtctg gcgcgggctc tcctgctcgg aaggcgggga aagccttcgc acgggagacg
     5461 tgacattcga cgtgtcggtt tcgaaggacg gccgcagggc cgagatgaag gcgatgtttg
     5521 agggaggctc cgcggacgtc gagggcatga acctgaagac gggcgccttc gaaatggcga
     5581 gcacgatcga ggagacgagg gctgaaaccc aagaggacga tgcgctctac tctcagagct
     5641 ggacgctgaa gtcgggtgag ctggattatg acggcgtgcg cgtccttgat gccctgacct
     5701 tcagggcgaa catgaccaac gtgcccgaac gcctcgtgga tgcgatggct ctgggcggcg
     5761 ccgtgagccc tgtgatcctc gagatggcgg cggagcaggc gatcatgcag ggcggcatga
     5821 cgttcgacct tgacgaatgc acggtgacgc gcggtgaggg gacgctgtcg gccgcagggc
     5881 gcgtggcaag ggacggagag aggatcggag ccttctccgt gcggttggat cccgagttcg
     5941 gagcggacgt caagggatgg cccgaagccg tagctgaact gaaggcgaac ggcagcctca
     6001 ggacggtcga aggacgcctt gaggcccatg tggagctttc gaccgatggc gttgccgtca
     6061 acggcgtgaa gctcgattcg ctttgacagg gacttagaca aaggaaaggc ctgcaggagt
     6121 gatcttgcag gcctttgact ttgttctggg cgggcggcga tcagacggca gcctgaatgg
     6181 agtcgcgcgt ggcgatggcg gtcttgcggg cggcttcctt ccagtcgtct tcgcggctcg
     6241 cgtagatgat ggcgcggccg gagttgatca ccatgccggt gccttcggcg gtcttgccgg
     6301 cctcaacgca ggcgcggatg tcgccgccct gagcgccgat ccccggcacg agcaggggaa
     6361 ggttcggcgc gatgcggcgc acgttggcga gttcggcggg ataggtggcg ccgacgacga
     6421 ggccgagttc gccgttgagg ttccactcgg tggcggcaag gcgcgcaagg cgttcgtaga
     6481 tcttctcgcc gttcacttca agcatctgga tgtcgtcgcc gccggggttg gaggtgcggc
     6541 agaggatgaa ggcgcccttg ttctcgtagg caaggtaggg cttgatcgtg tcgaagccca
     6601 tgaacgggga gagcgtcacg cagtcggcct cgtagcgctc gaaggcttcg cgggcgtagt
     6661 gctcggccgt ggagccgatg tcgccgcgtt tggcgtcgag gatcacggga accttcggat
     6721 gattggcgtg aatgtagtcg atcacggcct tgagttcggc ctcggcgccg caggaggcga
     6781 agtaggcgat ctgtggcttg aatgcgcaga cgaggtcggc cgtggcgtcg atgatgccgc
     6841 agcagaagtc gtagagggcc gtgctgcggc ccgcgagttc gtgcgggaag cgggtggggt
     6901 tggggtcaag cccgacgcag agcatggaac cggcgttctg ccagcgttcg ctgagcattt
     6961 cggtgaactt catggggtgc cttccgaagg tggttgtcaa tcgggcgcta tcttaacaaa
     7021 ttctttcgca ctgcggccgt tcctgaggaa ttgcgctatg ctcgacgaag cggcttccgg
     7081 agaggagcca cgtcaaaaac gccgtgtcat caggaggact gtgaagatga tcacacgcag
     7141 acagttcatc ggggccgcag gcctcatgac ggtcgccgca actgcgccgg ccttcgccta
     7201 cgagagccgc tggccgaaga agccgtcggt gcccaccgac ctcaatgccg aggagttctt
     7261 cacggagctc gccgagaagg gctcaggctt tgtcgtcggc aatgagaagg ccgatacggt
     7321 cgtgatgatg acgttcgaca cgcagtgccg ctggtgtgtc tgggagtacg agcagttcaa
     7381 gcccttcctt gaccgcgtga agttcgtctg gcatccggtt gcagtgctca atccctggag
     7441 cgagctgcag ggggccgcca ttctggccgc aaaggatccg aaggccatgt tcctcgagca
     7501 cgaggcgcac atgaaggatg ccgtcttcaa ggggctcgac gtgcgcaaga tggaaattcc
     7561 atttgaaaaa cgcgaggcag tgtggaccaa tactaaggta ttccgccgtg ccgcggcccg
     7621 agaggtgccg ttcggcgtca tgaagaagcc agacggcagc tatgtcgccg tttgcgagca
     7681 gacgggtgag tcatttgcca agcttctcgg caagtaaggc ggagcacaat aaggaagaga
     7741 ggcaaggaaa aggccggcga tcgaaagatc gtcggccctt gtctttttag gccgcccgtt
     7801 cctgagaacg ggcggcggtc ggctggtcag gcgtgatgaa ttacatcatg ccgggcatgc
     7861 cgcccatgcc ttccatgccg gcgggcatcg gcttgtcgtc ctgcggaatg tcggcgacca
     7921 tgcagtccgt cgtgaggatg agggacgcaa cggaagcggc gttctgaagg gcggtgcggg
     7981 tgaccttcgt cgggtcgaga acgcccattt caacgaggtc gccgtattcg ccggtctggg
     8041 cgttgtagcc gaagttgcct tcgccctgag cgacggtgtt caccacgacg ctcggttcgt
     8101 cgccggcatt ggcgacgatc tggcggagcg gttcttccat ggcgcgcaga acgatgcgga
     8161 tgccggcgtt ctgttcgtcg ttgtcgccct tgatgtcgcg gatggcctgc ttggcgcgga
     8221 tgagcgccac gccgccgccg gccacgatgc cttcctcaac ggcggcacgg gtggcgtgca
     8281 gggcgtcttc gacgcgggcc ttcttttcct tcatttcgac ttcggtggcg gcaccgacct
     8341 tgatgagggc cacgccaccg gcgagcttgg caacgcgttc ctgaagcttt tcacggtcgt
     8401 agtcgctcgt ggcggcttcg atctgcgtgc ggatgttcct gacgcggttt tcgatggctt
     8461 cggcctggcc ggcgccgtcg atgatcgtcg tgttttcctt cgtgacttca accttcttgg
     8521 ccgtgccgag ctgttcgagc gcggccttgt cgagcgtgag gccgacgttg gtcgagatga
     8581 ccgtgccgcc ggtcaggacg gcgatgtcct cgagcatggc ggcgcgacgg tcgccgaagc
     8641 ccggggcctt gacggccacg accttgagga tgccgcggat ggagttcacg acgagggtgg
     8701 cgagggcttc accgtcgatg tcttcagcga tgatgacgag cggacgggcg gccttggcga
     8761 cctgttcgag cacggggaga agttcgcgga tgttgctgat cttcttgtcg tgcaggagga
     8821 tgaacgggtt gtcgagaacg gcggcctgct tttccggctg gttgatgaag tacggggaga
     8881 ggtagccgcg gtcgaactgc atgccttcga cgacttcgag ttcgttcttg agggacttgc
     8941 cgtcttcaac cgtgatcacg ccttccttgc ccaccttgtc catggcttcg gagatgatgt
     9001 cgccgatttc agcgtcggag ttggcggaga tggcgccgac ctgggcgatt tccttgttgg
     9061 tcgtcgtcgg cttgctgatc ttgcggagct cttcgatggc ggcggcgacg gccttgtcga
     9121 tgccgcgctt gagatccatc gggttcatgc cggcggcaac gaacttcatg ccttcgtcaa
     9181 cgatggcctg ggcgagaacc gtggcggtcg tcgtgccgtc gccggcgttg tcgctcgtct
     9241 tggaggcgac ttcacgcacc atgcaggcgc ccatgttttc gaacttgtcc ttgagttcga
     9301 tttccttggc gacggacacg ccgtctttcg tcaccgtcgg agcgccgaag gcgcggtcga
     9361 gcacgacgtt gcggcccttc ggaccgagcg tgaccttgac ggcgttggcg agaacattga
     9421 tgccgcggac catcttggag cgggcgtcat caccgaaaag aacctgctta gcagccattt
     9481 ctgtaatcct ttactttctt agaaaaaaca aatgatgcaa ggcgctgtcg agcctttgat
     9541 taggcgagga cgcccatgat gtcttcttcg cgcatgacga gcaactcttc gccgtcgacc
     9601 ttgacggtct ggccggagta cttgccaaag agaacgcgat cgccgacctt gacgtcgaga
     9661 gcgatgatgc ggccggcttc gtcgcgcttg cccgggccga cggcgatcac ttcgccctgg
     9721 tcaggctttt caccggcgga gtcggggatg acgatgccga aggaggtggt cgtttcagca
     9781 gcaagacgct tgatgatgac gcggtcatgc aacggacgga tctgcatttg ttgttctcca
     9841 aataaaacgg tttccggcgg gcggtcgcat cgccccttct cagggcgggg ttcggctgcc
     9901 ggatgcaaaa aaacattgcc tcggcggacc gcacgggcgg ctttgcctta gcatttaaac
     9961 cctatatggg gatgcctcct tgaaaaacaa gggggctgtt gaagaatttt tacaggttgc
    10021 cgaggcgctc ctgggcgcct tggtggaaat gcctgcggcg agggtggaat gcggttttca
    10081 acgagaggct ccgcggccgt ttttgcggct ttcggaatga tggcggcggg cctttggtcc
    10141 gtttcgtgca gcggttgggc cgccgagcag ggaaggctcc cgcccatgct caggaaggcc
    10201 gccgcggact gcggcgtcga cccgagggcg gtgacggtgt cggtcgtcgc gctcgacgga
    10261 ctgaggatgc ccgagggcgg caggatgccc gtcgtccggc ccctgcttgc cgtcaacgcc
    10321 gacaggtcgg tggctcccgc ctcgaccgcg aagctggtga cgacgctcgc gggactcgaa
    10381 cttctgggcg cggactggaa gtggaggacg gggttctatg caaaggcgag gcctgatgcg
    10441 gagggccgcg tcaacggcct ctggctcagg ggcggcggcg atccgacgct cgtcgccgaa
    10501 aagttcgggc tcctcgtcga gcggatggcg cagatgggcg tcaggcgcat cgagggcgac
    10561 ctgacggtcg accgctctta cttcgacctg cccgaaggag atccctccgc cttcgacggt
    10621 cggggctccc gcccctacaa ccagctgccc gacgcggcgg tggcgggcta tcgcagcctt
    10681 tccttcgagt tcgttcccga cgagtcgtcg gggactgcgc gcatcatcag catgccgccg
    10741 ctctcggggc ttgaggttcc gtcgacgatc cgcctctcgc gcggcgcctg cggcgactgg
    10801 aagtcgacga tcggctacag gctcgaacac ctttccgacg gcaggctcgc agccaggttc
    10861 gagggctcgc ttcccctttc ctgcggaccg aagaccttca gcgtggtgtc cctttctcag
    10921 aacgaatacc tcgaacgtct cttcaggtgg tactgggagc gcgacgggcg cacctggacg
    10981 ggacacgtcg ccgaggggcg cgtgcccgaa ggcgccctga agctcgccga gcgggagtcc
    11041 gatgcgcttc ccgtcgtgac gacgcttgtc aacaagtggt cgaacaacct gatcgcccgt
    11101 cacatcttcc ttacgctcgg cacgctcaga aacgcgcccg acgaggcgga ctcaggatcc
    11161 cgaactgcag gcggagcctt tgcgcccatg gagcgccccc gccccggcgt cgacacggac
    11221 gacgcacgag cggtgctggc gggctggctg gctgaaaagg gcgtgcccga tggcgccgtc
    11281 atgatcgaca acggctcggg tctctcgcgc acctcgcgcg tgacggcgcg tgcaatgacg
    11341 cagattcttg cggcgggctg gctttcgccc cgcatgaccg agtacgcggc gtcgctgccc
    11401 gtctcgggcg tcgacggcac gatgagaaag cgccttgagg cgacgggccg cacgcatctg
    11461 aagacgggct acctgcagga cgtgcgctcg atcggcggct acgtctatgc gaagaacggc
    11521 aggcggtacg cgctttatgc cagcgtgcac ggcgagcgca acatggcagg cggtatcagg
    11581 ttcctcgaca aggtggtcga gtggacctac ctgcagaagt gaggccctgc atcgcaggca
    11641 cttcctgagg attcgggatc ctgaaacgaa gctcgccgcc cggtatggtg ccggacggcg
    11701 agcttttgct ttttctggat ttccagggaa ggcctcgtct tattgctgcc cgcccttcac
    11761 ggcgtcgagc acgatgcccg gcgtcaggag ctcggggagc acctgcacgg ttccgtccgg
    11821 cctgtagagg aggtagagcg gcacgccgga gcggccgaag cgggagagga tgtccgtgat
    11881 ggcgggatcg cggttcgtcc agtcgcccat gaggagcgtg acgtttccgt ccctgaaggc
    11941 ggtctgcact tcgtcgcggt caagagccgc gaccttgttg gcctgacagg tgacgcacca
    12001 ggcggcggtg aagtcgacga agacggggcg gccttcggca agggaggccc tgacggcctc
    12061 ttcggaccaa ggctgccaga cgcccctgcc ctgtgcggtg gcggcgcggt cgaattcgcc
    12121 cgtggcgatg acggcgattg cgccggccgc aagaaaggac gtgacgccca tcaggacgcg
    12181 gttgcgaccg cggccccact gctctcggcc gagcagccag agcgcgatgg cggcggagcc
    12241 gcagcccgca agcatcagaa gcatgccgtt gaggctgacc tgcttggaga gcacccagcc
    12301 gagccagacg acggcaagcg ccatcggaat cgccatgacc ttgcggaaga cgtccatcca
    12361 ggcccccggc ctcgggagca ttctcgacca gccggggaag atgcagagca gaagccacgg
    12421 aagcgccatg cccagaccga gcgagaggaa gacggcgatc gcctcgacgg cgggttgcgt
    12481 cacggcgtag cccagagcgg cgcccatgaa gggggccgtg caggggctgg cgacgatgac
    12541 ggcgaggatg cccgtgaaga acgagctcgc ggcaccggtt ctcggcgcgc tgcgcgcaac
    12601 gcgggtgtcg gcgagatggc ttgcggcagt gaattcaaag acgccaagca ggttgatcgt
    12661 gatggcgacg aagagcacga gcaggagcga gacgacccac ggactctgga gctggaagcc
    12721 ccaaccgagc gccatgccca cgcccctgag ggcgagaagc gcacccgcca gcacgcccat
    12781 cgtgacgagg acgccgcccg tgaaggcgat gccgtgcggc agcagtcttt cgcctctgcg
    12841 gtagccctcg atgagctgca ggatcttgag cgagaggatc gggaagacgc agggcatcag
    12901 gttgaggatg aggccgccga ggaacgcgaa gacgatggcg gtgagggtcg tcaccgaggc
    12961 gcccgagcct gcgacgggaa gtgcgccgga gaagatggcg ggcttgggca ccgcgccctt
    13021 ttccagcggg aaggaggttt cgatcgccca gccgcccgca gcggggccgc cgtcgcccac
    13081 gaagacgccc gagagcgtgg cgcgaggctc ctgcaggaac tcgggggcga gcgtcatgag
    13141 gatggaggcg ctaccgtcct cctcggtctg gtgctgcggc tcgtcggaga gcttcagcac
    13201 gcgctcgtca agcgggtaga aggaaaggct cttctcaacg tgcacttcgg aggcgggtac
    13261 cgtcaccttg aggcggtcct cctcgtagac ggcgcgcacg ccgcggatgt cggccacttc
    13321 gggcacgttg acgatggcgt tcgaaacgag ctcggcatcg gccgagggct tcggcgcgac
    13381 ctcgtagggc agcgtgattt cggccgtgcc ctcgccgggg atgcagacgt ccttgcaggc
    13441 gaggtaggag acgtggatgc tgatgcgcgc ggagcgtccc tcggggaagc gggggatctc
    13501 ggtctcgaac ggaaagacgg cggtgccgcc gtaagagaag ctcacgattc ccttgtcgtt
    13561 gtgacgttcg ggaagcggga agcgcggatc cgtgaggcga acgtccttcg gcgtcgtgaa
    13621 gctgaactgc gtcgtgaggc cggcgtcgcc tggcatgcgc cagtacgtgt gccagcccgg
    13681 cgcgtgctca agcaccacgg cgagaaggtt ttcggcctga ggcgtgaggc cctcgttctg
    13741 ggcgacgagc cgcacggtga cgctcggctc gggtgcgggc tgcgccgcgc ggtcgagcgt
    13801 cgagaggaga tcctgggccc cggaggccca ggtggaggcc gcggcggcgc agagcgcagc
    13861 tgcggcaagc caggtcttga cggaacccat gatcgtcctt tatgcaggcg ccgctcaggc
    13921 ggcgatttca gcaaacgtct cgcgagcggc ggcgatggtc gcgtcgatca cttcgtcggt
    13981 gtgcatgatc gagacgaagc ctgcctcgaa gacggacggg gcgaagtaga cgccgcgctc
    14041 gagcatgccg tggaagaagc ggttgaacgt ctccgtgtcg gacttcatca cgtcggcgaa
    14101 gccgtgcggg acggacggca ggaagtagag gccgaacatg ccgccgacgc tgtcggcgca
    14161 gaacgtgatg ccgttttcct gagcggcggc cgttaggccc ttgacgaggc ggtcggcggc
    14221 gcgggaaagc ttgtcctgga agcccggagc ctgaatgccc ttgagggtcg caagaccgca
    14281 ggcgacggcg acggggttgc cggaaagcgt gccggcctgg tagacgggac cgaggggagc
    14341 gatctgctgc atgatttcgc ggcgaccgcc gaaggcggca acgggcatgc caccgccgat
    14401 caccttgccg agcatcgtga ggtcgggctc gatgccgtag aaggcctggg cgccgccctg
    14461 agcgacgcgg aagcccgtca tgacttcgtc gacgatgagg acggcgccgt gcttcgtgca
    14521 gagctcgcgc atcgtgcgaa ggaattcggg gttgccgcgg accatgttca tgttgccggc
    14581 gacggcttcg acgatcacgc aggcgatgtc gtcgccccac tgggcgaagg cgtcctcaag
    14641 ctgctgcggg ttgttgtact cgagcacgag cgtgtgctcc gtcacggagg cgggaacacc
    14701 ggccgaggac ggattgccga aggtgagcat gccggagccc gccttgacga ggagagagtc
    14761 ggagtggccg tggtagcagc cctcgaactt gatgatcttg ttgcgacccg tgtagccgcg
    14821 ggcgaggcgg atggcggaca tgccggcctc ggtgccggag ctcacgaggc ggacctggtc
    14881 catggacggc acgagcttgc ggacctcttc ggcgatgagc gtctcgccct gcgtgacggc
    14941 gccgaaggag aggcccttcg agacggcttc gtcaacggcg gcgatgactt cgtcgttgtt
    15001 gtggccgagg atcatcgggc cccaggagag gatgtagtcg atgaactgct tgcccgtggc
    15061 gtcccacatg taggggccct tggcgcggtc gatgaagcgc ggggagccgc ccacggagcg
    15121 gaaggctcgc acgggggagt tcacgccgcc cggaatcgag atctgcgcac gctggaagag
    15181 ttcgtcattg aggtccattt gttcaactcc tgaaagcatg gaacgtgtcc gcggcggcct
    15241 ctcaagggtc gcggccgccg ggacggcaaa tgaaaaaaag ggcggccttc gaagcgggcc
    15301 gcccgaaaaa acaatgaatt ttaccgaaac agtctgaacg caccgttaac gggtgttgcg
    15361 tccgggacgg attcgtgcgg cgtcagaacg ccacgcccag ggagagcgtg aagtcggtcg
    15421 ccatgggctt gagcacgtag tcgtcgccgc gggcctcgac ggtgcccttg tagcgggcga
    15481 acgaggtgaa catttcggcg ttgacgaaga cgttcttcgt gaccgcgtag gtggcgccga
    15541 gcgacgtgac ggggtggaag gagtcgtcct tgccggcggg gcccttcatc ttggagtagc
    15601 ccagaccgcc gcccgcgtag aggcggaggc tgtcgagggc gtcgatgttg tactgaagct
    15661 ggacggccca ggtctggaag cggtaggact tgctcgtgtc cgtgcccgcc gtcttgatgt
    15721 catgctcgga gcggcggtac gaaacgtcgg tcgcccagtg gcggcccgag tagtggcgca
    15781 tgccgacgtg ccaggagctg tcgttcttga cgttgctcgg agagaactcc tcgccgttgt
    15841 ccatgtactc ccagccccag ccgccgtaga ggtagttgcg gtctgcgctc caggcgctgg
    15901 tggcgggcag cggaaggtcg cggcgcttgg cgtcgtcgat cgtgatcgtg gagtagaaga
    15961 agaacacgtt cgtgatgggg atgtacgtgc cgtagaagga gatgtcgtcc gtgccgatct
    16021 tcgcgacggg cagcggcatc gggaacggaa cccacatgta gtcgccgcgg gcggagacgg
    16081 cggcgaggta gcctgcgccg acgtgcaggc ccgtgcccgc gacaggccag cgggcgaccc
    16141 actggtagcc cgcgaccggt tcgggacggt agttagagtc gacgaaggtc acgaggaaga
    16201 aggagcgctc gttgccgcgg ttgtcgatga cctgacggcc gagacccatg ccgaagggat
    16261 agccgttctc ttcatgacgc ttgtcccagt cccaggtcgg gtggttcgtg taggtcggaa
    16321 cgacccagag gctgttgcct tccttcatga tggccttggc accgcggacg gtgtcctgcc
    16381 agaaggagcc gaagtcgccg gggcgttcgg cggaggagga cacgtagccg gccggcgtcg
    16441 tgtcgatcac ggcttcaagg aagccggcat gagcctggcc ggcggcgcag gccgcagccg
    16501 ccgtcgcggc ggcaagcgtc ttcattttga tgttcatggc tttgcggccg cccgcatcag
    16561 gcggggcggc cttcttgtaa gttgtctgaa tcgggagcgg agatgccgcg tccaggcgac
    16621 ggggtccgca gaacccgtca ttgtcgcaca aggcatcgag gcgggcgtca aaaaagcggg
    16681 aaaactcgtc gagcgagttg aaaaaatgcg ccaaagcggg tagagtgcgt cccctgactg
    16741 cgaaactttt ccagacgatc gtcggcggag caatccgcgg gaggaaagtc cggactccgc
    16801 agggcaccgt agcagctaac ggctgtccgc cgcgaggcga ggatcagagc aacagagacg
    16861 agccggcgtt ggggtcggtt gaaacgggca atctctacgg ggagcaacat caaataggca
    16921 gcctatggtt cggcccggac cggttgcggg tagatggctt gaggcgtcgc gcgagcgccg
    16981 tcctagacga atgatcgtca ctgcgccctc gggcgcggaa cataatccgg cttatcggga
    17041 gagtttccgg tcatcagaca acagaagagg ggttcggcac aagccgaacc cctcttcgtt
    17101 tatttgcaat cgtcagatcg atcaggcgag agccgattcg gcctcgcagc ggcgcacctt
    17161 gatgcggccg tcgacggcga tcggctcacc agtcttttcc gagatcatga gcggattgat
    17221 gtcaagttcg tcgatgaagg gaaggtcggt cacgagctgg gagagacgca ggagcgtctc
    17281 ctcaaccttg gcgatgtcgg cgggcgtcgt gccgcgggtg ccctgaagaa gcttgtaggc
    17341 cttgaccgag cggaccatgt cgcgagcgtc ggtgtccgtg acgggtgcga tgcggaaggc
    17401 gacgtccttc atgacctcga cgaagacgcc gccgaggccg aacatcagca tcgggccgta
    17461 ctgtgggtcg gtggcgatgc cacagaccat ttcgcgggag cccttgacca tttcctgaat
    17521 gatgacgcct tcaagcccgt cgagcaggcc gcgctcgtcg agcttggcga cgatgtcgcg
    17581 gtattcggcg cgaagcgcct cggctgagcc gatgttgacg cgcacgccgc ccacgtccgt
    17641 cttgtgggac gtggtcttgg aggtcatctt catgacgacg gggtagccga tgcggtcggc
    17701 gacggcgacc gctgcgtcct cgtctgcggc aaagccggcc ctgcacacgc gcacgccgta
    17761 ggcgtcgaga acgtcgatgc tctcgcgcgt cgtaagctgg tcgcggccgt cggcaagcgc
    17821 cgtgcggatg atgttgcggg cgcgttcgcc gtcaaccttg aagtcgggaa gcacgccctc
    17881 ggggcgctcg atccagaggc gctgctcgtt gaggcgcgca aggccctcga ccgcctcctc
    17941 gacgtacatg aagaagggca cgttgacgtc ggcggcggag aatgccgtga agaactcgct
    18001 cttcgtcatg aagacgccga cgacgggctt ttcgggatgt gcggccttga tctccatgat
    18061 cgcgcgggcg acgtcggtgt ccttgaggcc gaggaacggc aggtagatcg tgatgaccat
    18121 gtcgacgttc ggatcggcga gcaccgtttc gagcgtctgg cgatagtgct cgatcggagc
    18181 ggaggcgatc atgtcgacgg gattcctgac ggaggcggcg gcaggcagga agctccggag
    18241 cttcgctttc gtttcgtccg tgagctcggc catcttcatg cccttttcgc agatggcgtc
    18301 ggtgcccatg atgccggggc cgccggagtt cgtgacgatg gcgaccctgt cgcccttcgg
    18361 aatcgggcag ttggcgaaga ccttggcggt tgcgaagaga ttcttgaggg agaactcgcg
    18421 gatgacgccc gactggcgca ggagcgcgtc ggcggcgagg tcggcgcctg caagactgcc
    18481 cgtgtgggag gatgcggcgg aggcgcctgc ggccgagcgg cccgccttga gtgcgagcac
    18541 gggcttcttc ttggagaggc gcgaggcgat gcggcggaag tttgcgggat tctggatgga
    18601 ctccatgtag agaaggatct gcctgacgtc ttcgtccccc tcccagtatt caagcgcggt
    18661 ctcggcgttc acgtcggcct ggttgccgat cgaaatgaac tgcgcgaagc cgaggttgag
    18721 gtcgcggaga atgttgagga tgcccccacc gagcgcaccg gattgggaga cgaaaccgat
    18781 gttgccgcgc tcgggcagcg cttcggcaaa gcaggcgtcc atgcgcacgg cgggatgcgt
    18841 gttgacgacg ccgagacagt tcggccccac gcagcgcatg ccgtagcggc ggacgcgttc
    18901 gagaagctcg gcctcgagcc gggcgccttc agtccccgtt tccttgaagc ctgcggagat
    18961 cacgcacagg cccttgatgc ccttttcgtg gcactggtcg atcgtctgaa gcacgaagcg
    19021 tgcgttcacg atgatgacgg caaggtccac ttcgccggga atgtcgagca ccgtcgcata
    19081 ggcgggcagg ccttcgatga cgccgccctt cgggttgaca ggatagacgg ggccggcgta
    19141 gccgtattca cgcaggcgct tcatgatgtc cgagccgatg gtgtgctgct tggtggaggc
    19201 gccgacgacc gcgatcgagc ggggcttcat gatcttgttg aggatgttca taagtcgtgt
    19261 cagatcgaag ggtgggttcg agggggcctc tctcggtgaa aacgcgtgag gatgaggctg
    19321 tacggcgctt tcggagaggc aatccgtcaa gcctatcaca tgacggacgg cttgctgtgc
    19381 gggctaaggc gaattatcta gtccggagtc ccacgcggga gcggatgcgg ggcctcgaaa
    19441 aaagatgttg cctgccttga ccgtcggacg ccttgacgct aaagtgagac tgcagaagtg
    19501 ggaaaaagtg gggg
//
LOCUS       DBMDTI010000014        17442 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1048_length_17442_cov_2.331526, whole genome shotgun sequence.
ACCESSION   DBMDTI010000014 DBMDTI010000000
VERSION     DBMDTI010000014.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 17442)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17442)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17442
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1048_length_17442_cov_2.331526"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            87..752
                     /locus_tag="ACZPB6_01795"
     CDS             87..752
                     /locus_tag="ACZPB6_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1183243.1"
                     /translation="MAIRAGAAPYTDAWVIGLYLNSEKKELNGVITSKDKYKDRVFIN
                     EKGLRFTNENLPYLTDAVVKEKAVWGIADSSDPEKVKAALKYNDPKYAVRGDTWEALA
                     AAMGVPPDALARTMKDYNAACTTGDDKAFGKAKEFLKPFAKPPFFAVRVVPKTGGTIG
                     GVKVNDKFQVVREDGSVIKGLYAGGEVINRPYYNRVYTSGTGLGIAYTSGRIAGTNAA
                     AER"
     gene            complement(1079..3211)
                     /locus_tag="ACZPB6_01800"
     CDS             complement(1079..3211)
                     /locus_tag="ACZPB6_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012789.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR1183244.1"
                     /translation="MAQQKMRRLAVGREVFSEVALLSDKARQTFYTEITRFRMGSAVS
                     MKTLTGASVYEQVRLDGGISIIMKRIGDLFLALWAGPDPMAQEWADTHLCETNERTRS
                     VQVYEIADTMTVSAGPLDQAPVFEALSNDDLLDIGLPKDRLANIRSVKSEDDLVRMQA
                     QLPDRVYEMLTWYVQGEPWAKIVEAYKEDIADKQAVVETGDCLLDAGHFRIVETDEEL
                     RNIMDQPLAQWRVFLHPSQRKLVDRRWHGAARITGGAGTGKTVVALHRAKHLVELPDW
                     LSTDRLLFTTFTKNLAVDVEAQLRMICSREDMKRITVLNLDAWLATFLSQNGQGHRIV
                     YPGGSDGIYNAAWQSAIAKIPMHLGFPIAFYQDEWEQVVLPQHCHTARDYLFASRAGR
                     GNALTRAQRKEIWPVFEELRIRLSLANAVTPEDAACEAINILQKSYPEGLFRAVVCDE
                     IQDFKPDTLRLLRALTRDISKDEPFKEGDLFLVGDAHQRIYAKPVAFSSCGIEIRGRS
                     SKLRVNYRTTDEIRVQAEQVYRNKTVDDMEGAEDEKTGYASLRHGRKPIVKICSGVDE
                     ECRWICGEINTLTSGDRAYHSSDICITVRTNEMLARYEEILRGHGLEVRRISRDIPDD
                     PELPGIRLSTMHRIKGLEYKVVFVAGMNEGEFPHAPRDLQDRDAATRRLLLRAEEALY
                     YVACTRAVDVLFLSCGGNPGAFIRDKSE"
     gene            complement(3230..10060)
                     /locus_tag="ACZPB6_01805"
     CDS             complement(3230..10060)
                     /locus_tag="ACZPB6_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012492.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1183245.1"
                     /translation="MAEREGQKSYAMIPSIVERDIEEGLLSFINREFPISTPGFMTDR
                     KEDKTIVDAFVDDRENLVKGPWLEIRRPFRKSEVETKEVLPLLAGDVYQIGNDFTPYK
                     HQMAAFERLKAPEPKSTIVATGTGSGKTECFLYPILDYILHCNEEHDEKKRKGIKAII
                     IYPMNALASDQAKRLTKLCFEINRNAKASGLKGMLPTVGLYTGTPGVESRRMSDDGGN
                     YVCITDRKVLRQNPPDILLTNYKMLDYLLLRDEDRSLWQATTADTLRYLVVDELHTFD
                     GAQGTDLACLVRRLRSFLNLGKSLACVGTSATLGGDNGIADLQKYASEIFNADFSDPA
                     SIIQEDRLSAQEYFDSFGERRTVGRWPDAAAVSALRALPQDASVEDYVSTALLCWFPQ
                     ERFLSPVSPSYLEDTARQLPELLPHLDAFQRLMSREETIIHVRDLALEWHKVIASLAK
                     VSVDDVVLVIRSLVALVSLARLVGKNDRLLPFLNVRMQLWIRELSNMVATVSRHPRLL
                     PNADKKDEDELTLPILTCRDCNATAWGAVTEVDRKVISSHHKFYRAWFDKSDTVRLIY
                     PIEEDDYEDAIKKHPRHLFTFCQNDKSIFWLDPSTSREAAYHKACPICGADHGHLVVW
                     IPDTVKDYTNEAGAGAKRSDNCPCCGSVNSMRLFGARAVTLASAMVGHLNSSSANLDP
                     KLIAFSDSVQDAAHRAGFMQARGYTYTIRQAVAGLIRDNNRATSQRSSDPAKDPMSLF
                     NILSDLSDYWKERIGRRSELTGNHADDIALARFVTTFTPSDMMWRRPWLKFRAASSGM
                     PENRSASDRSTPHQQTAPVLCDDPHAFFEDLFSGKTGKNGKTPRDEWSDFSHDVAARL
                     RWEAFIELTLRAHAGRTLELGGIAALEPRSDLVQKAAASLQPILDQQVGGCRGITREE
                     TESFITGFLMHQKSRGAFDLTGIPGLEDFMSFVKTGNDYLYFNRSRVLPTYGKNFRPP
                     APLVMRMPSSLNRKSSGFFDAVLTSGRHENWYETWFAASAAYNKDDVLAAVDEAYARL
                     FDVLTKTGLMNVVYMTTTDATPVYLLNPSAWTIARKLEKAVCAHCGRWTLISPEDARL
                     WRQMPCLSKSCGGREHFIKDVEESSGLYLGTPARTTAREHTGNVESTERGLIEHSFSK
                     GHEPWDVNLLSATPTLEMGIDIGDLSTVLQASMPPQQANYLQRIGRAGRRDGNALAMT
                     ICGNNPHAQYFWTDPDKMLAGSVPAPGVFLHAMAVIDRQLLAHALTDWMSSIVNASIP
                     QKISSVLTNCNQDQYTPDRFPRGFFDYVRNHTDHILNEFCKAFVRPETGESIFCTAEK
                     ERLRASLVGGSLVPSIEDRIMGKLSTLTSELEKAQRSAKQLARDVQDLKKKGARDEAT
                     ESEIEEKQALEEAWKDLINREYVNKETLQVLTDEGLLPNYAFPEEGIKLDGIITRIRT
                     RSTDRQDPVKKDDSKRNVYKRLKFQRAASSGLLEIAPNNTFFVDEYVMHIDQVQLENE
                     KTEKWRFCPSCQHAILENDTTVSSACPECGDPQWRSNGQERNALKVKTVYAWADLRKD
                     RIVDNIEERRPLQQQKISLSSISSTAERHVFANKSIPGGFRFEYISAVTLRDFNFGMP
                     EEVESEFRIAQTKINGNGFSVCRGCGRLRNDENTKRNVRPEQHEASCPFVDSPDADDI
                     WINGLILYREFTSEAVRIKVPLTNDESPETTMHSLAAALHLGLRRYFHGSVDHLRIVP
                     MVEHKFDHIARRYILIHDTVPGGTGYLKELLSDKDNLFTLLQTAYKAITECGCGSEDG
                     CYQCVYQHRDSSTRPFISKSAAIRVLGLILAQQDSVARTSSADDDDLWPGESELERNF
                     INSLRNSQPFVKTAVERTVAGRKDFLIEMQSGRQWQMVMQEDIKNVFHPSRPDFVFRP
                     AHASERTPALTMAVFLDGWQFHAPTVQEDLVKRQSLINAGYRVWTLGWYDLPCSLEDE
                     NRPDVPNLLSRNSAPGYEILRQKLPLPSEADLRAHWSTDRNNFDRLLLWLNDPDQATK
                     DACALDLLSRGYFGTGTVEKLDSAAPVTSILKQGGAQMDVKKDSSVLPGAWSLVRNFS
                     YILGANPEFYTEHKEMPTNSPQAQSLMQFWAIANIMQLPQPIALLPVADPTNASEDLL
                     AQQPWSAAIEYIKDNHAPQRMFALFTGMPPIAHSTVGSEPSSNAWDDIDPDDLPDETL
                     VELVDKLKILDLPVPEIGCEYEDPVTHEVGELVFELYWPEQKVAVAWLEGEAETNVNG
                     IRVFPFGVDPERLSQAVKATGSSNSNNN"
     gene            complement(10060..15084)
                     /locus_tag="ACZPB6_01810"
     CDS             complement(10060..15084)
                     /locus_tag="ACZPB6_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183246.1"
                     /translation="MEWTGINNENEYYSAYFLSEGLADSLKDQFDAWAKLESENKAKA
                     EDAGEKDWQHAPNRALRMNARALLDELNDAFAETSDEEQRLQGWRSAAAAIGRCLELD
                     IKKTPEAAFVNGDDHMPLPLLCALHREGKAAEPYLWVFEALPDGRSVDFDDELDPLEM
                     HVSRSQFEDLPGFNSKVTAEFEDNWNKILEKHVFGCDNPPRWVILASPRSWLLIDRTK
                     FHRHSLLRFDWKELFSRREDRVLSLAAALLAKSSMENRNGTVLLDRIDEDAHKQAYGV
                     SESLKLSLRRAIELLGNEAATQIIEKARRDKKTVRRDETFANDLTVECLRYMYRILFL
                     LFVEARPDLAYAPTENDAFQSGYSFESLRDLEQKPLLTEEDRKGRYFHDSISMLVSFY
                     SQGTPFCSEDDTHQALEFGAATTDATFEIRPLKGSLFDMERTPNLNKVVFTNETLQQV
                     IRLMSLSSVGKGRRQNCGRISYAHLGIHQLGAVYEALLSYRGFFAQEDLYEVSADPSK
                     SDEFEAGYFVTKEEIDSDHYKDEQKVYETNEFGEKKLKVYPKETFIYRLTGREREKSA
                     SYYTPEILTQCLVKYVLKEYWETVIDKLPTEKEKAEHILKLRVAEPALGSATFLNEAV
                     NQLAVKYMEHAQKARDERLSQEDYNRELQKVKMYLADNNVYGVDLNPVAVELAQVSLW
                     LNALSSDKFVPWFGLQLHSGNSIIGCGKRVCKVDYEKGGKKIRISAAHELSGTRGEDE
                     IWQFLLPDFEMATYKDADFKKVYKTQIDTLNKKVKAFNKALSGNELEILQGLSAMVDE
                     HWDRWAEDLAKIRLSTTDPYAIYGHAASERTVLSYQEKNELAAKIRMGDGSYETGSFS
                     RLRTAMNYWCSLWFWPIEEADSFPTRAEYIADMMLILSSEFIGVEKAAGRQITLNLDD
                     SLEAEVSRFAEDDKGNLVESELDANPRIRISRQVSERLRFFHWPLRFADILYKTSDGS
                     ELGFDITLGNPPWRVPAWNSGAVVGDYLPYILFNKESASNIRELLLSTVDDDVRFIDA
                     HPELGESWRREYIEAAGTQNFCGSSSNYPELESCRMDLFKVFLPLSWKNTSSEGVQGF
                     VHPMTAFTETNASTLRKRAYEKARYIFQFTNEKKLFPEVHNETQFCEVIYGQQKGGTC
                     TAIMNIFHPTTIDESFASDGDSAVEGIKDSLGNWNLKGHPDRIIHLDSTAIATFAQIF
                     DSDPEAPILPNIHCQSMLSILEKFGAFPHRLNSISDELTISSMWNETTARVDGTIREF
                     SSHRTKTPNKYSTLILNGPHLSVGSPLFKTPFVKCSTNKAWAPIDLEAIPDNFIPRSK
                     YERGDISDEDYNNRQVCCEWDQVPEYERTNGEKSKGTNKYRPFDQHWRVAYRRMVGTD
                     SERTLTSALIPPGTAWIDSVNGIATNNLETLITITVNFSSIPFDALVRQMGKGNLLPS
                     LISSLPFIEYDQSTACAFVRTLCLNCLTTPYAELWEQCFKAEWKDDQWTQNAAGLDCT
                     WFQNLTPTWQRNNALRSDLSRRQALLEIDVLTAHAMKLTFKELLTLYRMRFRVMRSYE
                     ENTWYDQNGRIVFTTNAGLPGVGLPNKARSKDVAEGITYAINGQKCDERGLGFDNVKN
                     MKSGTVSKTFPDTTMSDEPQERTVTYVAPFFKMDREKDYETAWRVFSERFGWEKDESI
                     ADNGEK"
     gene            complement(15105..>17442)
                     /locus_tag="ACZPB6_01815"
     CDS             complement(15105..>17442)
                     /locus_tag="ACZPB6_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012403.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR1183247.1"
                     /translation="YVDKAIISIDTLKQSALYRTYLEQAYWDIILIDEAHNVAERSSG
                     SQRAALARLLSRRSDALIMLSATPHDGRPQSFASLIDMLDPTVIANPNDYTFDDFKDS
                     KLVIRRFKNDIRDQAQGAFPERDIKTIKVNASLAEQAVYQNLENIEFKHIDANRKTGS
                     VLFQTTLEKALFSSPAACISTCDNRIGKLRAAKTRVTEAGEDINTLTNFKQSLSDVDK
                     QSFSKYQALVSLLKNDEKSLDSEFVWDKKDPEDRLVIFTESRQTLAFLAQHLPPDLGL
                     KDTQFCVLKGDDSDKDLMDKVERFNKLESPIRLMLATDVASEGLNLHRLCHRLIHFDI
                     PWSLMTFQQRNGRIDRYGQQKKPIIRYLQTDVKTTAGKFIGDNHIMDLLIQKDKNAVA
                     NISDPGEFTGSKEEQEQQTAARIQNEAPPPAPSEEDFMSLLRAFSLGADQETTAGNAP
                     AAPQTSHQVDRSLLFTDYQFLKTGLTLRKKLPLERGGIATEVEFDDANEFVRLAPPKD
                     LEVRLQYLPKEVLPEHRKFELTARKDIVQRAIRDAASNPNSSWPAHQLLWELHPVMQW
                     MEDWAIGAFGRHAAPVVHVPFLPEGEFWTLLQGGYPNKRGGTPVHDWIAVKTDASGES
                     TVHSRKELMAIMKMPNAWTNDGLSLPVELLSEKLPGIVSLAEQHLLKRRAEYQNNIQD
                     LLSEKLAKLNRLREKQQRLIGMEDAPMNTSSLSKRQAKTLERTEYIEKRFKEAETYTK
                     DTYTLDDKPYVQVVALFVGKVVQQNAAEGADASQYQLL"
BASE COUNT         3377 a         4590 c         5548 g         3927 t
ORIGIN      
        1 tggcggtcac accgcagagc acaccggcca cggtgacggt caggatgccg atgatcatgg
       61 agccgggcac gcgcaggcac atcagcatgg ccatcagggc aggtgcggct ccctacacgg
      121 atgcctgggt gatcggcctg tacctcaaca gcgaaaagaa ggaactcaac ggggtcatca
      181 cgtcgaagga caagtacaag gaccgcgtct ttatcaatga gaagggcctt cgcttcacga
      241 acgaaaacct gccgtatctc accgacgccg tcgtgaagga aaaggccgtc tggggcatcg
      301 ctgattcgtc tgatcccgaa aaggtgaagg ccgctctcaa gtacaacgac ccgaagtacg
      361 ctgtcagggg cgacacctgg gaagcgctcg ctgccgcaat gggggtgccg cctgatgcac
      421 tcgccaggac gatgaaggat tacaacgccg cttgcacgac gggggacgac aaggcgttcg
      481 gcaaggccaa ggagttcctg aagccgttcg caaagcctcc cttctttgcg gttcgcgtgg
      541 tgcccaagac gggcggaacc attggcggcg tgaaggtcaa cgacaagttc caggtcgtgc
      601 gtgaggacgg ttcggtcatc aagggcctct atgcgggcgg cgaagtcatc aatcgtccgt
      661 actacaaccg cgtctacacc tcgggcacgg ggctcggcat tgcctatact tcgggtcgca
      721 tcgcaggtac gaacgcggcg gctgaacgct gatcgtctcc tgccctgaaa gacacacctg
      781 agcgcgcagg atgctcaaca agagcgcctg cgcgctttct gtattctctc atcgagtcga
      841 ctgaagcctg atcttgccgc ctctcgaatc tcttcggatg atgcagtagt gttcaagcag
      901 tgcctgaaga ggcttgccga gagggacaaa ggggcgaagg agaaagagaa gagcacccgc
      961 gaaaatggaa cgctcttctc tcaaggcgga acaagactgg atcggaacga gacaatcgtc
     1021 tgtcgccggc ccctgagaag agtgtatgca tcaaacacac actcttcgct ggaaagaatt
     1081 actcgctctt gtccctgatg aaggcgccgg gattcccgcc gcaggagagg aagagtacgt
     1141 cgacggctcg cgtgcaggcc acgtagtaga gtgcctcttc ggcgcgcaga agcaggcgtc
     1201 tggtggcggc atcgcggtcc tggagatcgc gcggggcatg ggggaactcg ccttcgttca
     1261 tgcctgcgac gaacaccacc ttgtactcaa gccccttgat gcgatgcatc gtggagagac
     1321 ggatgccggg cagttcgggg tcatcgggaa tgtcgcggct gatgcggcga acttcaagac
     1381 catggccccg aagaatctct tcatagcggg cgagcatttc gttcgtacgc acggtgatgc
     1441 agatgtcgct tgaatggtag gcgcggtcac cgctcgtgag ggtattgatt tctccgcaaa
     1501 tccagcggca ttcctcatcg acgccggagc agatcttgac gatgggcttg cggccgtgtc
     1561 gcagcgaggc gtagccggtc ttctcgtctt cagcgccttc catgtcgtcg accgtcttgt
     1621 tgcggtagac ctgttcggcc tgaacgcgga tttcatcggt cgtgcggtag ttcacgcgaa
     1681 gcttgctgga gcgaccgcgg atttcgatgc cgcagctgct gaaggcgaca ggcttggcat
     1741 agattcgctg gtgcgcatcg ccgacaagga acaggtcgcc ttccttgaag ggctcgtcct
     1801 tggagatgtc cctcgtgagc gcgcgaagga ggcggagcgt gtcgggcttg aagtcctgga
     1861 tttcgtcaca gacgacggct cggaagaggc cctcagggta ggacttctga aggatgttga
     1921 tcgcttcgca ggctgcgtct tcgggcgtca cggcgttggc gaggctgaga cgaatgcgga
     1981 gctcttcgaa gacgggccag atttccttgc gctgagcacg ggtgagggca ttgcctcggc
     2041 cagcgcggga ggcgaagagg tagtcgcgtg ccgtgtggca gtgctgcggc aggaccacct
     2101 gctcccattc gtcctgatag aaggcgatgg ggaagccgag atgcatgggg atcttggcga
     2161 tggccgactg ccaggcggcg ttgtagatgc cgtccgagcc gccgggatag acgatgcgat
     2221 ggccctgtcc gttctgggaa aggaaggtcg cgagccaggc gtcgagattg aggaccgtaa
     2281 tacgcttcat gtcctcccgg gagcagatca tgcgaagctg ggcctcgacg tccacggcga
     2341 ggttcttcgt aaaggtcgtg aagagcagac gatccgtcga gagccagtcg ggaagctcga
     2401 cgagatgctt ggcacgatga agcgcgacga cggtctttcc ggtgcccgcg ccgcccgtga
     2461 tgcgcgcagc gccgtgccag cgtcggtcga cgagcttgcg ctgggacgga tgcaggaaga
     2521 cgcgccactg cgcgagcggc tggtccatga tgttgcgcag ctcctcgtcg gtctcgacga
     2581 tgcggaagtg ccccgcgtcc agaagacagt cgccggtttc gacgaccgcc tgcttgtcgg
     2641 cgatgtcctc tttgtaggcc tcgacgattt tggcccacgg ttcgccctga acgtaccagg
     2701 tgagcatttc gtagacgcgg tcgggcaact gggcctgcat gcggacaagg tcgtcctcgg
     2761 acttgaccga gcggatgttg gcgaggcggt ccttcggaag gccgatgtcg aggaggtcgt
     2821 cattgctgag ggcctcgaag acgggagcct ggtcgagcgg acccgcggag accgtcatgg
     2881 tgtcggcgat ctcatagacc tgaacggagc gggtgcgctc gttggtttca cagagatggg
     2941 tgtcagccca ttcctgagcc atgggatcgg gacccgccca gagggcaaga aagaggtcac
     3001 cgatgcgctt catgatgatg gagatgccgc cgtcaagacg aacctgctcg tacacggagg
     3061 cgcccgtcag ggtcttcatg ctgacggcgc tgcccatgcg aaagcgggtg atttcggtgt
     3121 aaaaggtctg acgggccttg tcggagagaa gggccacttc gctgaagact tcgcgaccga
     3181 cggccagacg ccgcattttc tgttgtgcca ttgttcaaat cctttgttgt tagttgttgt
     3241 tgctgttgct gctgcctgtg gccttgacgg cctgagagag acgctccgga tcgacgccga
     3301 acgggaagac cctgatgccg ttgacattgg tctcggcttc accttcgagc catgcgacgg
     3361 caaccttttg ctcgggccag taaagttcga agacaagttc acccacctcg tgcgtgacgg
     3421 gatcctcata ttcgcacccg atttcgggca cggggaggtc aaggatcttg agtttgtcaa
     3481 caagttccac aagagtctca tcagggagat catctgggtc gatgtcgtcc caggcgttgc
     3541 tggaaggctc ggagccgacc gtcgagtggg cgattggcgg cattccggtg aacagggcga
     3601 acatgcgctg gggcgcatgg ttgtccttga tgtactcgat cgcggctgac cagggttgct
     3661 gggccagcag gtcttcagac gcattcgtgg gatcagcaac gggcaggagc gcaatgggtt
     3721 ggggaagctg catgatgttt gcaatggccc agaactgcat cagggactgc gcctgggggc
     3781 tgttcgtggg catttccttg tgctcggtgt agaactcagg gttcgcgccg agaatgtagg
     3841 aaaagttgcg cacaaggctc caggcgccag gaagcaccga cgaatccttt ttgacatcca
     3901 tttgggcgcc gccctgcttg agaatgctcg tgacgggggc ggcggaatca agcttctcga
     3961 ccgtgcctgt tccaaagtag ccgcgcgaga gaaggtcaag ggcgcaggcg tccttggtgg
     4021 cctgatccgg atcgttgagc cagagaagca gcctgtcgaa gttgtttctg tccgtagacc
     4081 aatgggccct caggtcagct tcgctcggaa gggggagttt ctgtctaaga atctcgtagc
     4141 ctggagcgga gtttctgctg agcaggttcg gcacatcggg tctgttttca tcctccagcg
     4201 agcagggcag gtcgtaccag ccgagtgtcc agacgcggta gccggcgttg atgaggctct
     4261 gtcgtttcac gaggtcttcc tgcacggtcg gggcgtggaa ctgccagccg tcgaggaaca
     4321 ccgccatggt gagggcgggt gtgcgctcgg aggcgtgtgc agggcggaag acgaagtcag
     4381 gccggctcgg gtggaagacg ttcttgatgt cctcctgcat gaccatctgc cactggcggc
     4441 cggactgcat ttcgatgaga aagtccttgc ggcctgcaac cgtgcgctcg acggccgtct
     4501 tcacgaacgg ctggctgttg cgcagtgagt tgatgaagtt gcgctccagc tcgctctcac
     4561 ccggccagag atcgtcatca tcggcggacg aagtgcgggc aacggagtcc tgctgtgcaa
     4621 ggatgaggcc gagcacgcgg atggcggcgg acttcgagat gaaggggcgg gtactgctgt
     4681 cgcggtgctg gtagacgcac tgatagcatc cgtcctcgct gccgcagccg cattcggtga
     4741 tggccttgta ggcggtctgt aggagcgtaa agaggttgtc cttgtcggag agaagctcct
     4801 tgaggtagcc cgtgccgccc ggcaccgtgt cgtgaatgag aatgtagcgg cgtgcgatgt
     4861 ggtcgaactt gtgctcgacc atcgggacga ttctcagatg gtcgacggag ccgtgaaagt
     4921 agcggcgaag ccccaggtgc agggcggctg ccaacgagtg catcgtggtc tcgggagact
     4981 cgtcgttcgt gagcggtacc ttgatgcgga cggcttcgct cgtgaattca cgatagagga
     5041 tgagtccgtt gatccagatg tcgtctgcat cgggactgtc gacgaagggg caggaggcct
     5101 cgtgctgctc ggggcggaca ttgcgcttcg tgttctcgtc gttgcgcagg cggccgcagc
     5161 cgcggcagac ggagaagccg ttgccgttga tcttcgtctg ggcgatcctg aattcggact
     5221 cgacttcctc gggcatgccg aagttgaagt cacgaagcgt cacggccgag atgtactcga
     5281 agcggaaacc gccgggaatg ctcttgttgg cgaacacatg gcgttcggcc gtcgagctga
     5341 tgctgctgag gctgattttc tgctgttgca acggacggcg ctcctcgatg ttgtcgacga
     5401 tgcggtcctt cctcaggtcg gcccaggcgt agacggtctt caccttgagc gcattgcgct
     5461 cctggccgtt gcttcgccac tgcgggtcgc cgcattcggg acaggcactg ctgacggtcg
     5521 tgtcgttttc cagaatcgca tgctggcagc tcgggcagaa gcgccacttt tcggtcttct
     5581 cattctcaag ctgcacctgg tcgatgtgca tgacgtattc gtcgacgaag aacgtgttgt
     5641 tcggggcgat ttcaagaagt cccgacgaag cggcgcgctg gaatttcagg cgcttgtaga
     5701 cgtttcgctt cgaatcgtcc tttttcacgg gatcctgccg gtctgtcgaa cgtgtcctga
     5761 tgcgcgtgat gatgccgtcg agcttgatgc cctcctcggg gaaggcgtag ttcggcagaa
     5821 ggccttcgtc ggtaaggacc tgaagcgtct ccttgttgac gtactcgcgg ttgatgaggt
     5881 ccttccaggc ctcctcgagg gcctgcttct cttcgatttc ggactcggtc gcctcatctc
     5941 tggcgccctt tttctttaga tcctgaacgt ccctggcaag ctgcttggcg gagcgctggg
     6001 ccttttcaag ctcggatgtc agcgtggaga gcttgcccat gattcggtcc tcgatcgagg
     6061 gaacgaggga gccgccgacg agggaggcgc gcaggcgttc tttttcggct gtgcagaaga
     6121 tggactcgcc ggtctctggg cggacgaagg ccttgcagaa ctcgttgaga atgtggtccg
     6181 tatggtttcg cacgtaatcg aagaagccgc gcggaaagcg gtccggcgtg tactggtcct
     6241 gattgcagtt ggtcaggacc gagctgatct tctggggaat ggacgcgttg acgatgctgg
     6301 acatccagtc ggtgagtgcg tgcgcaagca gctggcggtc aatcacggcc atggcgtgca
     6361 ggaagacacc gggagcgggc actgaacccg caagcatctt gtcgggatcg gtccagaagt
     6421 actgggcatg cggattgttg ccgcagatcg tcatggcgag cgcattgccg tcgcgtcgac
     6481 cggcacggcc gatgcgttgc aggtagttgg cctgctgcgg cggcatggac gcctgaagga
     6541 cagtggagag gtcgccaatg tcgatgccca tctcgagcgt tggcgtggcc gagaggagat
     6601 tgacgtccca cggctcgtgt cccttgctga agctgtgctc gatcagaccg cgctcggtgc
     6661 tctcgacgtt gcccgtatgc tcccgcgccg tcgtgcgtgc gggagtgccg agatagagcc
     6721 ccgaggactc ctcgacgtcc ttgatgaagt gttcgcgacc gccgcagctc ttggagaggc
     6781 acggcatctg gcgccagaga cgggcatctt cgggcgaaat gagcgtccat cggccgcagt
     6841 gagcgcagac ggccttctcg agcttgcgcg cgatggtcca ggcggacgga ttgagcaggt
     6901 agacgggggt tgcatcggtc gtcgtcatgt acacgacgtt catgagccct gtcttcgtca
     6961 gcacgtcgaa aagacgtgca taggcttcgt cgacggcggc aagcacatca tccttgttgt
     7021 aggcggctga cgcggcgaac catgtctcgt accagttttc atgcctcccg gaagtcagaa
     7081 cggcgtcgaa gaaccctgac gacttgcggt tcaggctcga gggcatgcgc atgaccaaag
     7141 gagccggcgg acggaagttc ttgccgtagg tgggaagcac gcggctcctg ttgaagtaca
     7201 gatagtcgtt gcccgtcttc acgaagctca tgaagtcttc aagaccggga atgcccgtga
     7261 ggtcgaaggc gccgcggctc ttctggtgca tcagaaagcc cgtgatgaag ctctctgtct
     7321 cttcccgagt gatgccgcgg cagccgccga cctgctggtc cagaatgggc tgcaggctgg
     7381 cagcggcttt ctgcacgagg tcggagcggg gttcgagtgc cgcaatgccg ccaagctcaa
     7441 gcgtacggcc tgcatgggct cgaagtgtca gttcaatgaa ggcctcccag cgcagtcgcg
     7501 ccgcgacgtc gtgagagaag tcactccatt cgtcacgcgg cgttttcccg ttcttgcccg
     7561 tcttgcccga gaacaggtct tcgaagaagg cgtggggatc gtcgcatagg acgggagccg
     7621 tctgctgatg gggggtgctc cggtctgaag cggaccggtt ttcaggcatg ccgctcgatg
     7681 ccgcacggaa cttcagccag gggcggcgcc acatcatgtc ggatggggtg aaggtcgtga
     7741 cgaagcgggc gagcgcaatg tcgtccgcat gattgcccgt gagttcggac cttctgccga
     7801 ttcgttcctt ccagtagtcg gagaggtcgg aaagaatgtt gaagagggac atcggatcct
     7861 tggcgggatc cgaagagcgt tggctcgtgg cgcgattgtt gtcgcgaatg aggccggcga
     7921 ccgcctgacg gatggtgtat gtgtagcctc gagcctgcat gaagcccgcc ctgtgcgcag
     7981 cgtcctggac gctgtcggag aaggcgatga gcttggggtc aaggttggcg gaacttgaat
     8041 tgaggtggcc gaccatggcg gatgcgagcg tgaccgcccg ggcaccgaag agacgcatcg
     8101 agttgacgga tccgcagcag gggcagttgt cggagcgttt ggcgccggcg ccagcctcgt
     8161 tggtgtagtc cttcacggtg tccggaatcc agacgacaag atggccgtgg tctgcaccgc
     8221 agatcgggca cgccttgtga taggcggctt cacggctcgt gctcggatcg agccagaaaa
     8281 tacttttgtc gttctgacag aaggtgaaga ggtgcctggg gtgttttttg atcgcgtctt
     8341 cgtagtcgtc ttcttcgatc ggatagatga gcctgacggt gtcactcttg tcaaaccacg
     8401 cacggtagaa cttgtggtgg ctggagatca ccttcctgtc gacttctgtc acggcgcccc
     8461 acgccgttgc attgcagtcg cggcaggtaa ggatcggaag cgtgagctcg tcctcgtcct
     8521 tcttgtctgc attgggcagc aggcggggat gacggctgac ggtcgccacc atgttggaga
     8581 gctcgcggat ccagagctgc atgcggacgt tgaggaaggg cagcagcctg tcgttcttgc
     8641 cgacaagacg ggccagggag acaagggcga ccagacttct gatgacgagc acgacgtcgt
     8701 caaccgagac cttcgcaagc gacgcaatga ccttgtgcca ttcgagcgca aggtcgcgga
     8761 cgtgaatgat ggtttcctca cggctcatca ggcgctggaa ggcgtcaaga tgcggtagaa
     8821 gttcgggcag ttggcgggcc gtgtcctcaa gatagctggg ggagacggga gacaggaagc
     8881 gctcctgcgg gaaccagcag aggagtgcgg tcgagacgta gtcctcgacg gaggcgtcct
     8941 gcggcagcgc gcgcagcgcg gagacagcgg ccgcgtcggg ccatcggccc acggtccggc
     9001 gttcgccgaa gctgtcgaag tattcctgcg cggacaggcg gtcctcctga atgatcgagg
     9061 caggatccga aaagtccgcg ttgaagatct cggacgcgta tttctgaagg tcggcaatgc
     9121 cgttgtcacc gccgagcgtg gcggaagtgc cgacacaggc gaggctcttc ccgagattga
     9181 ggaagctcct cagacgcctg acgagacagg cgaggtccgt gccctgagca ccgtcgaacg
     9241 tatgcagctc gtcgaccacg agatagcgca gcgtgtccgc ggtcgtggcc tgccagaggc
     9301 tcctgtcctc gtcgcgcagg agcaggtagt cgagcatctt gtagttcgtg agcaggatgt
     9361 cgggcggatt ctgcctcaga accttgcggt ccgtgatgca gacgtagttg ccgccgtcgt
     9421 cgctcatgcg gcgtgactca acgccgggtg tgcccgtgta gagaccgacc gtggggagca
     9481 tgcccttgag gcctgaagcc ttggcgttcc tgttgatctc aaagcagagc ttcgtcagac
     9541 gcttcgcctg gtcggaggcg agggcgttca tcggatagat gatgattgcc ttgatgccct
     9601 ttctcttctt ctcatcatgc tcttcattgc agtgaaggat gtagtcgaga atcgggtaaa
     9661 ggaagcactc tgtcttgccg gagcccgtgc ccgttgccac gatcgtggac ttgggttcgg
     9721 gtgccttcag acgctcgaat gcagccatct ggtgcttgta cggcgtgaag tcattgccga
     9781 tctgatacac atcgcctgcc agaagcggaa gtacttcctt cgtttcaacc tcggactttc
     9841 ggaaggggcg gcggatttcc agccagggcc ccttcacaag gttttcgcga tcgtcgacga
     9901 acgcatcgac gatcgtcttg tcctcctttc tatccgtcat aaagccgggc gtggagatcg
     9961 ggaactctct gttgatgaag gagagcaacc cttcctcaat gtctcgttcc acgatgctcg
    10021 gaatcatggc gtagcttttc tgtccttccc gttcagccat tatttttctc cgttatcggc
    10081 gatggattcg tccttctccc agccgaagcg ttcggagaag acgcgccaag ccgtctcata
    10141 atctttctcg cggtccattt tgaagaacgg cgcaacgtag gtcacggttc tctcttgcgg
    10201 ctcatcgctc atcgtcgtgt ccgggaaggt cttcgagacc gtgccggact tcatgttctt
    10261 cacattatcg aagccaaggc cgcgctcatc acacttctgg ccgttgatgg catacgtgat
    10321 gccttcggct acatccttcg aacgcgcctt gttgggaagg ccgacgccgg gcaggcctgc
    10381 gtttgtcgtg aagacaatgc ggccgttctg gtcgtaccac gtgttttcct cgtagctgcg
    10441 catgacgcgg aagcgcatgc ggtatagcgt cagaagctcc ttgaacgtga gtttcatcgc
    10501 atgagcagtc agaacgtcaa tctcaagcaa agcctgcctg cggctcaggt ccgaacgaag
    10561 ggcgttgttg cgctgccagg tcggcgtgag gttctggaac caagtgcaat cgagtccggc
    10621 ggcattttga gtccactgat cgtctttcca ttctgctttg aaacactgtt cccacaattc
    10681 ggcgtatgga gtggtcagac agttcaaaca aagagttcgt acaaatgcac aggcggtgga
    10741 ttgatcgtac tctatgaaag gtaagctgga tatcagagaa ggtaacagat ttccttttcc
    10801 catttgtcta acaagtgcat caaagggaat ggaggaaaaa ttaaccgtta ttgtgatgag
    10861 ggtttccaaa ttgttcgtgg caatgccgtt cacactgtca atccaagctg tcccgggtgg
    10921 aattaacgca gaggtcaaag tgcgctctga gtcagtgccc accatgcgtc ggtaagccac
    10981 tcgccaatgt tggtcgaagg gccgatattt attcgttcct tttgatttct ctccatttgt
    11041 tctttcgtat tcaggaacct gatcccattc gcaacaaact tgtctattgt tgtagtcttc
    11101 gtcagaaatg tcgcctcgtt cgtatttcga tcgtggaatg aaattatcag gaattgcttc
    11161 aagatcaata ggtgcccatg ccttgttggt tgaacatttt acaaaaggag ttttaaacaa
    11221 gggagatcct actgacaaat gcggtccatt caagattagt gttgaatact tgttcggtgt
    11281 ttttgtgcga tggctagaaa attctcgaat tgttccgtca actcttgctg tggtttcatt
    11341 ccacattgat gaaatcgtca attcgtcact aatgctgttc aaacgatgag gaaaggctcc
    11401 aaatttctcg aggatggaca gcatggattg acaatggatg ttcggaagaa tgggcgcttc
    11461 tgggtcgctg tcgaatattt gagcaaacgt agcgattgcc gtgctgtcca agtggatgat
    11521 tcgatccggg tgccccttta gattccaatt tcctaaagag tctttaattc cttcgactgc
    11581 cgagtcccca tcgctcgcga atgattcgtc aattgttgtt gggtggaaga tgttcattat
    11641 tgccgtgcat gttccccctt tttgttgtcc gtaaatgact tcacaaaatt gagtttcgtt
    11701 atgaacttca ggaaatagtt tcttctcatt tgtaaactgg aatatgtatc ttgctttttc
    11761 gtatgcgcgt ttacgaagag ttgaagcatt tgtttctgta aatgctgtca tgggatggac
    11821 aaagccttgc acgccttctg aggatgtgtt tttccaggat aggggaagaa aaaccttaaa
    11881 aaggtccata cgacaacttt cgagttcagg atagtttgat gaactaccgc agaagttttg
    11941 cgttcccgct gcttcgatgt attccctcct ccaggattcc ccgagttccg ggtgcgcatc
    12001 gatgaaacgg acatcgtcat cgacagtaga aagcaacagt tcacgaatat ttgaggcgct
    12061 ttccttgttg aagagaatgt acggcaagta atcaccgact actgctcctg agttccatgc
    12121 gggaacacgc cacggcggat tgcccaacgt gatgtcaaag ccaagctcgc ttccgtccga
    12181 tgtcttgtac agaatgtcgg caaagcgcaa cggccaatgg aagaaacgca ggcgttccga
    12241 cacttgacgc gagatgcgga tacgggggtt ggcatcgagc tcggattcaa caagattgcc
    12301 cttgtcatcc tcggcaaagc gagagacttc tgcttcgagg gagtcgtcaa gattgagcgt
    12361 aatctgacgt ccagccgcct tttccacacc gatgaattca ctcgacagga tgagcatcat
    12421 gtcggcgatg tactcggcgc gtgtcgggaa gctgtccgct tcttcaatgg gccagaacca
    12481 aagcgagcac cagtagttca tggccgtgcg cagacgtgag aaactgccgg tttcataaga
    12541 gccgtcaccc atgcggattt ttgcggcgag ctcattcttt tcctgatagg agagaaccgt
    12601 gcgctcggac gcagcgtgcc cgtaaatggc atacgggtcg gtcgtgctca ggcggatctt
    12661 ggccaggtct tcggcccatc tgtcccaatg ctcatcaacc atggcactga gcccttgcag
    12721 gatctcgagt tcatttcccg acaaggcttt gttgaacgcc ttgacctttt tattcagcgt
    12781 atcgatctga gttttgtaga ccttcttaaa gtctgcgtct ttgtaagttg ccatttcgaa
    12841 gtccggcagc aggaactgcc agatttcatc ctcgcctctt gtgccggaga gttcgtgggc
    12901 cgcactgatg cggatctttt tgccgccctt ttcgtaatcg accttgcaga cgcgcttgcc
    12961 acagccgatg attgaattgc cgctgtgaag ctgcaggccg aaccacggca cgaacttgtc
    13021 ggacgagagc gcattgagcc acagcgagac ttgggcaagc tcgacggcca cggggttcaa
    13081 gtcgacgccg tagacgttgt tgtcggcgag gtacatcttg accttctgga gctcccggtt
    13141 gtagtcctcc tgagagaggc gctcatcccg ggctttctgt gcgtgctcca tgtacttgac
    13201 ggcgagctgg ttgacggctt cgttcaagaa ggtggcggag cccagtgcag gttcggcaac
    13261 gcggagcttg agaatgtgct cggccttttc cttttcggtc gggagcttgt cgatgaccgt
    13321 ctcccagtat tccttgagaa catacttcac aaggcactgc gtgagaattt caggcgtgta
    13381 gtaggacgct gacttctcgc gttcacggcc agtcagacgg tagatgaagg tttccttcgg
    13441 atagaccttg agcttttttt cgccgaattc gttggtctcg tagaccttct gctcatcctt
    13501 gtaatgatcg gagtcgatct cctccttcgt cacgaagtag ccggcttcga actcgtcgga
    13561 cttcgacggg tcggcggaga cttcgtacag gtcttcctga gcgaagaacc cgcgatagga
    13621 aagcagtgct tcgtaaacgg cgccgagctg atggatgccg aggtgagcgt aggaaatgcg
    13681 cccgcagttc tgacggcgtc ccttgccgac ggaggagagg ctcatgaggc ggatgacctg
    13741 ctggagcgtc tcgttggtga agacgacctt gttgaggttg ggcgtacgct ccatgtcaaa
    13801 gagcgagccc ttgagcggac ggatttcaaa ggtggcgtca gtcgtggctg caccgaattc
    13861 gagtgcctga tgcgtgtcgt cttcactgca gaagggagtt ccttgcgaat agaaggagac
    13921 cagcatggag atggagtcgt ggaagtaacg gcccttgcgg tcctcttcgg ttagcagggg
    13981 cttctgttcc aggtcgcgaa ggctttcaaa cgagtagccg gattggaagg catcgttttc
    14041 ggttggtgcg taggcaaggt cgggacgcgc ctcgacgaag agcaggaaga gaatgcggta
    14101 catgtagcgc aggcattcca ccgtcagatc gttagcgaac gtttcgtcgc gcctgacggt
    14161 cttcttgtcg cggcgggcct tctcaatgat ctgcgtggcc gcttcgttgc ccagcagttc
    14221 gatggctcgg cgaagcgaga gcttgagcga ttcggaaacg ccgtaagcct gcttgtgcgc
    14281 atcttcatca atgcggtcga gaagtacggt gccgttgcgg ttttccatgc tgctcttggc
    14341 gagaagcgcg gcggcaagcg aaagaacgcg gtcttcacga cgagagaaga gctccttcca
    14401 gtcaaagcgc aggaggctgt gacggtggaa cttcgtgcgg tcaatgagaa gccaggagcg
    14461 cggggaggca aggatcaccc agcgcggcgg attgtcgcaa ccgaagacgt gcttttcgag
    14521 aatcttgttc cagttgtctt caaactctgc cgtcaccttg ctgttgaatc caggcaggtc
    14581 ttcgaactgg gatcgggaga catgcatctc aagcgggtca agttcgtcgt caaagtcgac
    14641 ggacctgccg tcaggcaggg cctcaaaaac ccaaaggtac ggctcggcgg ccttgccttc
    14701 acggtgtagc gcgcaaagca ggggaagcgg catgtggtcg tcaccgttga cgaacgcagc
    14761 ctccggtgtt ttcttgatgt cgagctccag acatcggccg atggccgcgg cagcagagcg
    14821 ccacccctgc agacgctgct cttcatccga cgtttcggca aaggcatcgt tgagctcgtc
    14881 aaggagagcg cgggcattca tgcggagagc gcgattgggg gcatgctgcc agtccttttc
    14941 gccggcgtct tctgccttgg ccttgttttc agactcaagc ttcgcccagg catcaaactg
    15001 gtccttgagc gaatcggcca gaccttcgga aaggaagtag gcgctgtaat actcgttttc
    15061 gttgttgatt ccggtccatt ccatgacgtg gatcctttcc gtggttagag aagctggtac
    15121 tgggaggcgt ccgcaccttc agcggcattt tgctgcacga cctttcccac aaacagtgca
    15181 acaacctgaa catagggctt gtcgtccaac gtgtaggtgt cctttgtgta ggtttcggct
    15241 tccttgaagc gcttctcgat atattccgtg cgctcgaggg tcttggcctg acgtttgctc
    15301 aacgaagatg tattcatcgg agcgtcttcc atgccgatga gccgctgctg cttttcacgc
    15361 agtcggttga gcttggcgag cttctcgctg aggaggtcct gaatgttgtt ctggtattcg
    15421 gccctgcgct tgaggagatg ctgttccgca agggagacga tgccgggcag tttttccgac
    15481 aagagctcga cagggagcga gagaccgtcg ttggtccagg cattgggcat cttcatgatt
    15541 gccatcagtt ccttacggga atggacggtg ctctcgccgg atgcgtcggt cttgacggca
    15601 atccagtcgt gaacgggcgt gccgccgcgc ttgttcgggt agccgccctg aagcagcgtc
    15661 cagaattctc cttccggaag gaagggaacg tgcacgacgg gagcggcatg acgaccgaag
    15721 gcaccgatgg cccagtcctc catccactgc atgacggggt gaagctccca gaggagctga
    15781 tgagcaggcc aggacgagtt tgggttggag gccgcgtcac gtatggcgcg ctggacgatg
    15841 tcctttctgg cggtcagctc gaacttgcgg tgctcgggca gcacttcctt cgggagatac
    15901 tgcaggcgca cttcaagatc cttcggcgga gcaaggcgca cgaattcatt ggcatcgtcg
    15961 aattcgactt cggttgcaat gccgcccctt tcgaggggaa gcttcttgcg aagcgtgagc
    16021 cctgtcttga ggaactgata gtccgtaaac aaaagagaac ggtcgacctg atggctcgtc
    16081 tgaggagcag cgggggcgtt gcctgcggtt gtttcctgat ctgcgcccag ggagaacgca
    16141 cgaagaagac tcatgaagtc ttcctcgctg ggagcgggag gcggagcctc attttgaatc
    16201 cgggcggcgg tctgctgttc ctgctcttcc ttggaaccgg tgaattcgcc gggatcgctg
    16261 atgttcgcga cggcgttctt gtccttctgg atgagcaggt ccatgatgtg gttgtcgccg
    16321 atgaacttgc ctgcggtggt cttcacatcg gtctgcagat agcggatgat cggctttttc
    16381 tgctgaccat agcggtcgat tcgaccgttg cgctgctgga aggtcatgag agaccaggga
    16441 atgtcgaaat ggatgagcct atgacagaga cgatgaaggt tcagaccttc ggacgccaca
    16501 tcggtggcaa gcatgaggcg aatggggctt tcgagcttgt tgaagcgctc gaccttgtcc
    16561 atcagatcct tgtcgctgtc gtcacccttg aggacgcaga actgcgtgtc cttcaggccg
    16621 aggtcgggag gaaggtgctg ggcgaggaag gcaagggtct gacggctttc ggtaaagatc
    16681 accagacggt cttccggatc ttttttgtcc caaacgaatt cggaatccaa ggacttctcg
    16741 tcgttcttga gaagagagac gagagcctga tatttggaga aggactgctt gtcaacgtcg
    16801 gaaagcgact gcttgaagtt cgtcagcgtg ttgatgtctt caccggcctc agtcactcgg
    16861 gtcttggctg cacgcagctt tccgatgcgg ttgtcgcagg ttgagatgca ggcggccgga
    16921 ctcgagaaga gagccttctc aagcgtcgtc tgaaaaagca ctgagcctgt cttgcggttg
    16981 gcgtcaatat gcttgaattc gatgttttcg aggttctgat agacagcctg ttcagccagg
    17041 gacgcattga ccttgatggt cttgatgtcg cgttcgggga aggcgccctg agcctgatcg
    17101 cggatgtcgt tcttgaagcg gcggataacg agttttgagt ccttgaagtc gtcaaacgtg
    17161 taatcgttcg gattggcgat gaccgtcgga tcgagcatgt cgatcagact ggcgaagctc
    17221 tggggacgtc cgtcatgcgg agttgccgaa agcatgatga gcgcatccga gcggcgggac
    17281 agcaggcggg cgagtgcagc gcgctggctg ccggagctgc gttcggcgac attgtgggcc
    17341 tcgtcgatga ggatgatgtc ccagtaggcc tgttcgagat acgtgcggta aagggcgctc
    17401 tgcttcagag tgtcgatcga gatgatagcc ttgtcgacat ac
//
LOCUS       DBMDTI010000015        17272 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1056_length_17272_cov_2.298760, whole genome shotgun sequence.
ACCESSION   DBMDTI010000015 DBMDTI010000000
VERSION     DBMDTI010000015.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 17272)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17272)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17272
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1056_length_17272_cov_2.298760"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="ACZPB6_01820"
     CDS             <1..1488
                     /locus_tag="ACZPB6_01820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1183248.1"
                     /translation="ARGPLGIGRLRRAAGSVFVVRADDTRYNQVSDLRTAFVAAAGMA
                     SFDGWITALDEVANVSQYPKNFFGKTTFLGASGLSVARAVLERDADVGILRVCELETL
                     TAAGLVEPGSLRVVGEKPQQGLACVSSTELFPGLALAAQTYVPEDVRRRIAAAVYSMP
                     VPESGYRWTLANDYRNVRRVAEKFAYAPTKKASDARFSVVEERYKYALVLGFLILAAS
                     MLYSVSVSKIVARRTKALVKVIDEKDALEDHARRDRERLSQLERAGIVSELSSMIAHE
                     LRQPVASLINYADGLSLYLGGKGHDPVIDEATREIGRQAERVSSIVERVRNYARQKEG
                     LQREIDFCAVVKSAYSTFRSGAASSSVRVSADLVDAAPVEGDPLELELFVVNTLKNAL
                     HALQSGSVEHPEIRLRLKADLSVENNPRWVLEVEDNGLPLDDKKFAELSHPVTSEKFE
                     GLGLGLSICRVIAERHRGRLTFRRAEPRGLVVSLSLPQVRTAEAA"
     gene            1503..2183
                     /locus_tag="ACZPB6_01825"
     CDS             1503..2183
                     /locus_tag="ACZPB6_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1183249.1"
                     /translation="MFTSMPNPDLYSPLARIIDDEETVRNSEAFMLRMGGINAVAYES
                     AEEFLARDDLRHPGCLVLDVRMPGMSGLELQEEMTRRGIDLPILFLSGHGDISMAVST
                     LKRGAEDFCEKPVEPAKLRESVRRMIIKNIAHRREAIDVERKRERFAMLTEREQLVVR
                     LVARDMLNKQIAAELDIQEHTVKIHRSNACRKLEVRSALELHHYLSAIGELAEDQKAP
                     EDRDFTDD"
     gene            2344..3456
                     /locus_tag="ACZPB6_01830"
     CDS             2344..3456
                     /locus_tag="ACZPB6_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR1183250.1"
                     /translation="MLETYLKDLEEVVNIDCGSNTCDGVTRVAEIMKRHYDEIGFATE
                     LVDLGDKAGRGLFATNKPGAEKFDIMFNAHLDTVYPDGTVAQRPFRVEDGTVYGPGCA
                     DCKAGVIAIFHALKNARKEDLDRLAIAVCYNPDEEVSSLSSREWLATMAKKARRAVVS
                     EPGRASGAFVRSRKGRSVWDVTFHGIAAHAGNNPQDGRSAILAAARFTIEVTKLQDLE
                     GKGTSVTVGVIQGGTVCNTVPETCSIRIDTRRWNDEDGREIDEGIEKLAAMNWGEGIT
                     VDARRASVSPAMPCTEKTRELVELINEAARLEGFDAGWVDAGGGSDANRIAQAGTPVV
                     DGVAPAGGCFHSEREYLRVDTIENRVRMLSRFLTLL"
     gene            3781..6012
                     /locus_tag="ACZPB6_01835"
     CDS             3781..6012
                     /locus_tag="ACZPB6_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR1183251.1"
                     /translation="MEQYYLVLLAFLGVIAVIDLFVGVSNDAVNFLNSALGCRISSFR
                     TTLWVASIGVLAGATFSSGMMEIARSGVFHPQLFTFSELMIVFFAVMVADVILLDAFN
                     SLGLPTSTTVSIVFELMGAAFAAALFKLIDAGAPLSDVADYINSSKSLGIVSGILISV
                     VVAFISGAVVQYLARLVFTFKFEKAYRRIGAIYGGIALTAIIYFLVMKGAKGASFMRP
                     EWIQWINANTAVILFSLFTGLTVLFQVLITVFRVNIFKIIILAGTFSLAFAFAGNDLV
                     NFVGVPLAAWDSWKEWQASGAADNAFMMGGLLKPSTAPTAFLLLSGFVMVLTLWFSKK
                     AHRVIQTSINLSSSRSGTQEQFGASAPGRMIVRGAMGLGKVISQIIPMPMQRGIASRF
                     EPLPVVKGEIPLPFDYVRASINLIVSAILIASATSLKLPLSTTYVTFMVAMGSSFADG
                     AWDRESAVYRISGVLTVISGWFLTALSASSLAAVVAVLVFWGGEAAAIILGFGAIFLL
                     VRSNLKTREDDVTLSDESRSVYDAAYVGQLLSKHVQKSLSETLRVLSRIHENFTADRE
                     RELTKVKASATDVFEDAIEARSVYYRMVAAEAAPSKADFDARYLYLRACTNTREVSRS
                     LQNLAKLARDHVANRHRVGSNEITNDIGTLVADIRTLVNAREDHADVTALRNRAADVI
                     TRIEDLQRRLMEAQPRGSMTIRCCEFHLSYLLVLRELVNHYEIASLLEEQIDALARGA
                     KAA"
     gene            complement(6028..7416)
                     /locus_tag="ACZPB6_01840"
     CDS             complement(6028..7416)
                     /locus_tag="ACZPB6_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR1183252.1"
                     /translation="MNRLYDRMTRPAPNKTPAWTGRSVPLLSEEGFAEALERLEAKIV
                     ALEALAKTPPKGAAALGDLVRMADEARDLGVSLRTVCRARQSSNAADTLPGTWLSSVK
                     RLSFRLEEASGGILSAVRELPDEVRARAPFEAWLSPLAVRGSQSSSLFEAVMLPLGSQ
                     QSHLVATMKLRIPSEDGHMISLSYAQATSVLKTSPDNRFRRSVFALFNAWFAEHSAAF
                     ADLLNAVLGWKLHEAHAAGRDFMTAAFEADRITPAAQLAMMKALELRRENARLTITER
                     ARLLGERQLHVTQILGSVEGMHASAPEGLNGLDEVCGSLSSAMACTDPAFGRYLEEAL
                     ENHWIDARSMSQRAGGTWCEDLPAYNATAVFSTLPSGTAGAFQFAHPLGVGFMHKAQH
                     GEQAPLKRLPYSVIEIFGQLAVTMLERHMARKLEGSTEADLLRWQLMRRASNMLLMVP
                     SRTRAAIPLDCV"
     gene            7681..9069
                     /gene="dcuC"
                     /locus_tag="ACZPB6_01845"
     CDS             7681..9069
                     /gene="dcuC"
                     /locus_tag="ACZPB6_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HIR1183253.1"
                     /translation="MIGILIGCLTLVVVGWAIVKGKYAPLVLFASGAFMLACAVFFDT
                     GTFMPKKALPTGNDYLNIIEFIRYMFSNRLAGLGFLIMTMVGFASYMTHIGANDAFVN
                     IAIKPLSIIRNPYILIFVAFMLGKAVSMVITSAVGLGVLCLALMGPALAALGINKKAI
                     GAVFVTSGACSLVLLGGSTAASAKACDLSILDYVFLFKIPAGIPTVLAIGLAHVFWQR
                     WLDRREGWNCEEHKGETLVFSEDVKRPSTAAPKIYAFLPFLPMVLVVVFSQYCISSIK
                     LDITALMLLCVIIGMVCEVIRWRGNYDKIAAGLKVFLQSMGKALSGVVALVIAAGVFA
                     EGFKALGMLDAIVDVAHNVGAGAVGMSILFVAITTLVTIIAGSNGASFYPLVEMVPKI
                     ARDMGVNPVTLVLPMHQASTIARPLSPVAGVVVAIAGMLQMNPFELIKRASVPCIVGL
                     IVHHVSVFWLAI"
     gene            9091..10887
                     /locus_tag="ACZPB6_01850"
     CDS             9091..10887
                     /locus_tag="ACZPB6_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR1183254.1"
                     /translation="MTEFNSGAPEWQMDDAYGSFADPRWTRGVERVRELIAEIARLTK
                     DLSSDETLRLGLEAYEEALTIESSMSAFAKCTGAKDTTDASAMAASNFTAGLHHDLEA
                     AAGPLFETLAAKPDDSPLWSMKPFSHWRFVIRERQADWHRRLSATDADFYASIEVMAF
                     APLGSLHKSLQKLVDFDARNAAGETVRVRAAKLVAILKGDPDPVLRQSAGEAMQEWYA
                     RHGELYAGLLNELHGIRLAGFERAGVEPVSVSLAQNRMSRECLEAIRTAILRNTDWIR
                     EGFRMRARVLGTPKMAYWDVLAPAPETSGGTPRLMPYEEGIGIVKRSLGRVNPDMTAF
                     VDLMLENRWIDAKPSDRKVGGAFYSRFNEFRIPRVFSTYLGSITSVLQQGHELGHAFH
                     YWKMRDLPMIETEFPMTLTETASTFNEAVIRRALLEEAEGAERFKMLWQDVRSAGNFL
                     VNTMVRMDFELAFLEERRRGVVPAGRCCELMREVWTRWYGDDVTPDVWLWAHKLHYYK
                     TDQFIYNYPYAVGYLMSLALMREWEERGDDFYGFYVAMLRDTGRMTVDEIIRRHFGAD
                     AADPGFWEGAMQSARRSTEALGALIEARGAEA"
     gene            10999..12096
                     /locus_tag="ACZPB6_01855"
     CDS             10999..12096
                     /locus_tag="ACZPB6_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024996.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR1183255.1"
                     /translation="MQYSKIALAVMLTAAATAVSATDIQIYGRVDAGLMFEDADGGER
                     SLTMKSGGRTSSRLGFNIKEDLGNGNFVKVYLENGFKVDDGSFSTADTLFDRRAILAV
                     QGAWGELGLGRAGTVQSTAAPYSMGSIKWDVFGTSYSNSSIGTTFANSSRVDNAINYV
                     SPVMNNLKFGLSYSTGDKDDDDANEWQDKGHTFAAAMNYIGDNLFLGVTFANVDNKHP
                     GGTAADGRAYQVGGWYRVVPTVRIFAGAGYQTAWSTGAKLAATKATAMTADQKGGWDG
                     YSLALGFEHLSGPHKTLIGAQYFDGELANNSDVDFKRAVYGAAYEYSFAKNVIAYVAV
                     NYSDASGAGSKVDDASAKDTVQGFLGMSYLF"
     assembly_gap    12150..12159
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12488..14344
                     /locus_tag="ACZPB6_01860"
     CDS             12488..14344
                     /locus_tag="ACZPB6_01860"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HIR1183256.1"
                     /translation="MNFKTKLAVLAIGAAMGSMAFNASACSTVIVGKDASATGNILVG
                     HNEDNGGRILTAQHWVPAATHKAGEMIKFEEAAASIPQVEKTFGFFWSQTFDPTGASF
                     SDGFVNENGVTIVTNACTGIYKDDIMPTKDGGIGYGIRRLMAERATSARHAVDIAIEL
                     LGKYGYFSEGRTYTIADPNEAWQLAIHQGNTWVARRVQDNEIIYIPNNFMMDKVDATD
                     TKNVIVAPGMIERAIKNGRYKPAKEGVYSDFNYRVAVAPAERRSADYNSSRNNLAWNK
                     IIGKNITDPEQFPYSAVATKKWTVDDVKDLLRTHEHDIQEQDAQWTHTNSLGICRATT
                     HESEVFEMNANPLLITGYRALARPCESPYIPFYPLARPAEGTAFMSWDKATAEHFKGT
                     PEYFGWRSEWPVTTFVAAANTYDFQRQDQKDVRAFVEKLEAGWEKDVPAVTAHAKTLL
                     KVSKEKAVEYLHAYNVRMLNDAQAAVAEKLEEKAPWTLAVMADSINPKSDERVEVVLF
                     SSGKLDATKADPKQTWGGVGRASIGNKITMSQKLAQPVKAEARDIDGDGLKDMVFTFT
                     QKGLAQNMLAGANYDIWLHTYVDGKRVAAMDTAFIETEGYKGPAKRTQNADL"
     gene            complement(14464..>17272)
                     /gene="tamB"
                     /locus_tag="ACZPB6_01865"
     CDS             complement(14464..>17272)
                     /gene="tamB"
                     /locus_tag="ACZPB6_01865"
                     /inference="COORDINATES: protein motif:HMM:NF047952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0089705 - protein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HIR1183257.1"
                     /translation="GPVPAKFLLAADPATAGLPFSVTLVSPSLKLPLEGANPADLTSL
                     SAFSLRMSGDVRNWKLDAGLGVKAPGTPSATAGLKGRGTLTSLTLDELTAKTAGGTGR
                     VTGEITWVDALRWRGDMKLANLDVSHFAPSVKAVLSGSSRTSGRIDKKGWSVELPEFD
                     VKGRIDNAALDVHGSAIAQSPMKVYVPDVSVSLDANKLTGRAMLDGKNIKADIKVDAP
                     DLGRNMKELAGSASGFVTIGGTLSEPVAKADLTATGLRYGDDVRLEALMLRGDISAKP
                     SGEVGGSMMLHASNGALSESLVLHDIVARLEGTDRKHELTVNLSGEPVNAELKLAGAF
                     DTKSLVWKGTLSNGTVSTPIGSWHQDKAAGLVYKASSSEAVLEPHCWVGKPGDVCIDD
                     KLRAGEKGSATLRLRKWELDVLKPWLPPLTTVAGTVNGTAKAKWDLSSGSLPEIDALV
                     DAGGMTVTQTTGEEPLVVHLDTVSLKARTDKSRASAELDVKIRDNADLKATVAVIDPA
                     ETRRLDGRILFNGGDLSIVNTLIGSDEKVEGRLNVDVALSGTLLKPLLNGGIALTDAR
                     VSEGAVPLVMKPSNIRIDFAGDTSTLEGRLETSQGALDLAGDAQWRDMAHPTANVHAS
                     GSDILITVPPYAKVTVSPDVSLAASSERVTLNGRIEVPAAQITVADLPATATGVSSDE
                     VLLDRHMKPKVSKTATIPIDSNIVVHMGDQIFIDAFGLKAKLSGDLKVTQDARRGLGL
                     NGQVNVDDGRFHAYGQDLIVRTGHVIFAGPPSRPQLNIEAIRNPEAIEDDVTAGIRVT
                     GNAMRPKVTVFSEPALSQQQTLSYLLRGQGLDTSSDDNSMITSALVGLGVSQTGRIIG
                     AIGNAVGIKDLGVDTAGVGESSQVVVSGYILPGLQLKYGVGIFDSLATITLRYRLMPR
                     LYLEAASGVNQAFDVLYSFEY"
BASE COUNT         3118 a         5733 c         5266 g         3145 t
ORIGIN      
        1 gcccgcggcc ctctcgggat cggtcgcctg cggagggccg cgggctccgt cttcgtggtg
       61 agggcggacg acacccgcta caaccaggtc tccgatctga ggaccgcctt cgtggcggcc
      121 gcgggcatgg cttccttcga cggctggatc acggcgctcg acgaagtggc caacgtttcg
      181 cagtatccga agaacttctt cggcaagacg accttcctcg gggcctcggg cctgtccgtt
      241 gctcgggccg tactcgagcg tgacgccgac gtggggattc tgcgcgtgtg cgagcttgag
      301 accttgacgg ctgcgggact ggtggagccg ggcagccttc gcgtcgtggg tgagaagccg
      361 cagcaggggc tcgcctgcgt gagctccacc gagctctttc cgggccttgc gctcgccgcc
      421 cagacctacg tgcccgagga cgtcaggcgc cgcatcgcgg ctgcagtcta ctcgatgccc
      481 gtccccgagt cgggctaccg ttggacgctc gccaacgact acaggaacgt gcgccgcgtc
      541 gcggagaagt tcgcctatgc gcccacgaaa aaggcgtccg acgccaggtt ctcggtggtc
      601 gaggagcgtt acaagtacgc gctcgtcctc ggatttctca tcctcgccgc ctcgatgctc
      661 tacagcgtgt cggtgagcaa gatcgtcgcg cgccgcacga aggcgctcgt caaggtgatc
      721 gacgagaagg acgcacttga ggaccatgcg aggcgcgacc gcgagcgcct ctcgcagctc
      781 gagcgcgcgg gcatcgtctc ggaactctcg agcatgatcg cacacgagct aaggcagccc
      841 gtcgcctccc tcatcaacta cgccgacgga ctttcgcttt atctcggcgg caaggggcac
      901 gaccccgtca tcgatgaagc gacccgcgag atcgggcgtc aggccgagcg cgtctcgagc
      961 atcgtcgagc gtgtgcgcaa ctatgccaga cagaaggagg gcctgcagcg cgagatcgac
     1021 ttctgcgccg tcgtgaagtc ggcgtactcc accttccgtt cgggcgccgc aagctcgtcc
     1081 gtccgggtct cggccgacct cgtcgatgcg gctcccgtcg agggggatcc actcgaactc
     1141 gagctcttcg tcgtcaacac cttgaagaac gcgctccacg cgctgcagtc gggcagtgtg
     1201 gagcaccccg agatcaggct gcgcctcaag gctgacctca gcgtcgaaaa caacccgcgc
     1261 tgggtgctcg aggtcgagga caacggcctg ccgctcgacg acaagaagtt cgccgagctc
     1321 tcgcatcccg tcacgagcga gaagttcgag ggcctcggtc tcggactctc gatctgccgc
     1381 gtcatcgccg aacgccacag ggggcgcctc actttcagac gcgccgagcc ccggggcctc
     1441 gtcgtctcgc tttcgcttcc gcaggtcagg accgccgaag ccgcatagga gtcactagaa
     1501 tcatgttcac cagcatgccc aatcccgatc tctactcgcc gctcgcgcgt atcatcgacg
     1561 acgaggagac cgtacgcaac tccgaggcct tcatgctccg catgggcggc atcaacgccg
     1621 tcgcctacga gagcgccgag gagttcctcg cgagggacga tctgagacac cccggatgcc
     1681 tcgtgcttga cgtgcgcatg cccggcatga gcgggctcga gctccaggag gagatgactc
     1741 gcaggggaat cgacctgccg atcctctttc tctctggcca cggcgacatc agcatggcgg
     1801 tctcgaccct caagcgtggt gccgaggact tctgcgaaaa gcccgtcgag cccgcgaagc
     1861 ttcgcgagag cgtcaggcgc atgatcatca agaacatcgc gcaccgccgc gaggcgatcg
     1921 acgtcgaacg aaagcgcgag cgcttcgcca tgctcaccga gcgcgagcag ctcgtcgtca
     1981 ggctcgtcgc ccgagacatg ctcaacaagc agatcgcggc cgagctcgac atccaggagc
     2041 acaccgtcaa gatccaccgc tcgaacgcct gccgcaagct cgaggtgcgc tccgcacttg
     2101 aactccacca ctacctctcc gccatcggcg aactcgccga ggaccagaag gcgcccgagg
     2161 acagggactt caccgacgac tgagaatggg gagtccgact ctcccgaaaa tgcatcgtgt
     2221 gccccgcttg aaccaaggta caaggcgggg cgattcgttt tgatccacaa tctctcccat
     2281 cggacgggcg gaccacatgc ccgttcacgg accaacaacc gaatcaccaa gcggagagag
     2341 cgaatgcttg aaacctatct gaaggacctc gaagaagtcg tcaacatcga ctgcggcagc
     2401 aacacctgcg acggcgtcac gcgcgtcgcc gaaatcatga agcgccacta tgacgaaatc
     2461 ggcttcgcca ccgaactcgt cgacctcggc gacaaggccg gccgcggtct cttcgccacg
     2521 aacaagccgg gcgctgaaaa gttcgacatc atgttcaacg cccatctcga cacggtctac
     2581 cctgatggca ccgtcgccca gcgtccgttc cgcgtcgagg acggcacggt ctacggcccg
     2641 ggctgcgccg actgcaaggc cggcgtcatc gccatcttcc acgccctcaa gaacgcccgc
     2701 aaggaagacc tcgaccgtct cgccatcgcc gtctgctaca accccgacga agaagtgagt
     2761 tcgctctcct cccgcgaatg gctcgccacg atggccaaga aggccaggcg cgccgtcgtc
     2821 tccgaaccgg gccgcgccag cggcgccttc gtgcgctccc gcaagggtcg ctccgtctgg
     2881 gacgtcacct tccacggcat tgccgctcac gcaggcaaca acccgcagga cggccgctcc
     2941 gccattctcg ccgccgccag gttcacaatc gaagtcacca agctccagga cctcgaaggc
     3001 aagggcacga gcgtgaccgt cggcgtcatc caaggcggca cggtctgcaa cacggttccc
     3061 gaaacctgct ccattcgcat cgacacccgt cgctggaacg acgaggacgg ccgcgagatc
     3121 gacgaaggca ttgaaaaact cgccgccatg aactggggcg agggcatcac cgttgacgcc
     3181 cgccgcgcct ccgtctcccc ggccatgcct tgcacggaaa agacgcgcga actcgtcgag
     3241 ctcatcaacg aagccgcccg cctcgaaggc tttgacgcgg gctgggtcga tgccggcggc
     3301 ggttccgacg ccaaccgcat cgcccaggcc ggcacccccg tcgttgacgg tgttgcccct
     3361 gccggcggct gcttccactc agaacgagaa tacctccgcg tcgacacgat cgaaaaccgc
     3421 gtgcgcatgc tctcgcgctt cctgaccctt ctctaaaaag aacaggtcaa aacaagtcaa
     3481 ggggtgaccg ccctcaaacg gtcgtgctga ggatgcagtt cgcccgcctt tcaaacgaaa
     3541 ggcgggcgat tctgcgttcg ggaaccgtga atgccgtcgt gtcacacaaa tgagatgaac
     3601 gggctgaaac gacagtgcga gtgacggtct cgggaagaac ggcgccgcct gaggacgctc
     3661 gcggcgcagg agaaagtgtc gaatatgacg tctgtcatca aattgtcata attccgtcac
     3721 gaagccgtca cgcggagcgg tcagaatgcg ggcgttgtct taccaacgct tacatctgac
     3781 atggaacaat actatctggt gttgctggcc ttcctcgggg tcatcgcagt cattgacctt
     3841 ttcgtcggcg tctcgaacga cgccgtcaac tttctgaatt cggccctcgg ctgccgcatc
     3901 tcctccttcc gcacgacgct ctgggtcgcc tcgatcggcg tgctcgcggg cgcgacgttc
     3961 tcctcgggca tgatggagat cgcccgctcc ggcgtcttcc atcctcagct tttcaccttc
     4021 tccgagctga tgatcgtgtt tttcgctgtc atggtagcgg acgtcatcct gcttgacgcc
     4081 ttcaattcgc tcggtctccc gacgtcgacc acggtctcga tcgtgttcga gctcatgggc
     4141 gccgcctttg ccgccgcgct cttcaagctc atcgacgcgg gcgctccgct ctccgacgtc
     4201 gccgactaca tcaattcgag caagtcgctc ggcatcgtct cgggcattct gatctccgtc
     4261 gtcgtcgcct tcatttcggg cgccgtcgtg cagtacctcg cccgcctcgt cttcaccttc
     4321 aagttcgaga aggcctaccg acgcatcggc gccatctacg gcggcatcgc gctcacggcc
     4381 atcatctact tcctcgtgat gaagggcgcc aagggcgcga gcttcatgcg tcccgagtgg
     4441 atccagtgga tcaacgccaa cacggccgtc atcctcttct cgctcttcac gggcctgacg
     4501 gtgctcttcc aggtgctcat cacggtcttc cgcgtcaaca tcttcaagat catcattctg
     4561 gcgggtacgt tctccctcgc gttcgccttt gcgggcaacg acctcgtgaa cttcgtgggc
     4621 gtgccgctcg ccgcctggga cagctggaag gaatggcagg cctcgggtgc cgccgacaac
     4681 gccttcatga tgggcggcct tttgaagcct tccacggctc cgaccgcctt cctgctcctt
     4741 tcgggcttcg tgatggttct cacgctctgg ttctccaaga aggcccaccg cgtcatccag
     4801 acctccatca acctttcttc gagccgctcc ggcacgcagg aacagttcgg cgcctccgcg
     4861 cccggccgca tgatcgtgcg cggcgccatg gggctcggca aggtgatcag ccagatcatc
     4921 ccgatgccga tgcagagggg catcgcctcc cgcttcgagc cgctccccgt cgtcaagggc
     4981 gagattccgc tgcccttcga ctacgtgcgc gcctcgatca acctgatcgt ctccgccatc
     5041 ctgatcgcct ccgccacgag cctcaagctg ccgctctcga ccacctacgt gaccttcatg
     5101 gtcgccatgg gctcgagctt cgccgacggc gcctgggacc gagagtccgc cgtttaccgc
     5161 atctcgggcg tgctcaccgt gatttcgggc tggttcctga ccgcgctctc cgcgagctcg
     5221 ctcgccgccg tcgtcgccgt gctcgtcttc tggggcggtg aggccgccgc catcatcctc
     5281 ggcttcggcg cgatcttcct cctcgtgcgc tccaacctca agacccgcga ggacgacgtg
     5341 acgctctccg acgaaagccg atccgtctac gatgccgcct acgtgggcca gcttctctcg
     5401 aagcacgtgc agaagagcct ctccgaaacg ctccgggtgc tctcgcgcat tcatgagaac
     5461 ttcacggccg atcgtgagcg cgagctcacg aaggtcaagg cgagcgccac cgacgtcttc
     5521 gaggacgcca tcgaggcccg cagcgtctac taccgcatgg tcgccgccga ggccgcgcct
     5581 tccaaggccg acttcgatgc ccgctacctc tatctgcgcg cctgcaccaa tacccgagag
     5641 gtgagccgct ctctgcagaa cctcgccaag cttgcccgcg accacgtggc caaccgccac
     5701 cgcgttggca gcaacgagat cacgaacgac atcggcacgc tcgtcgcgga catccgcacg
     5761 ctcgtcaacg cccgtgagga tcatgccgac gttacggcgc ttcgcaacag ggcggctgac
     5821 gtcatcaccc gcatcgagga tcttcagcgc cgcctgatgg aggcccagcc ccgtggttcg
     5881 atgaccatcc gctgctgcga gttccatctc tcctacctcc tggtgctccg cgaactcgtc
     5941 aaccattacg agatcgcctc gctcctcgag gagcagatcg atgcgctcgc aagaggtgcg
     6001 aaggctgcct gaggccgcat cagcaattca aacgcagtct agtgggatcg cggctctggt
     6061 ccgtgagggc accatgagga gcatgttcga tgcccgacgc atgagctgcc agcgcagaag
     6121 gtcggcctcg gtcgagcctt cgagttttcg ggccatgtgt ctttcgagca tggtgacggc
     6181 cagctgtccg aagatctcga tcacggagta ggggaggcgc ttgaggggcg cctgctcgcc
     6241 gtgctgggcc ttgtgcatga agcccacgcc gagcggatgt gcgaactgga atgctcccgc
     6301 cgtgcccgac gggagggtcg agaagacggc cgtcgcattg taggcgggaa ggtcttcgca
     6361 ccaggtgccg cccgcgcgct gcgacattga tctcgcgtcg atccagtggt tttcgagcgc
     6421 ctcctccaga tagcggccga aggcggggtc ggtacaggcc atggccgagg acagcgagcc
     6481 gcagacctcg tcgagcccgt tgagcccttc gggcgccgag gcgtgcatgc cctcgacgga
     6541 gccgagaatc tgcgtgacgt gcagctgtct ttcgccgagc aagcgagccc tttccgtgat
     6601 cgtgagtctc gcgttctcac gcctgagttc aagcgccttc atcatggcga gctgcgctgc
     6661 gggcgtgatg cggtcggcct cgaacgccgc cgtcatgaag tcccgtcccg cggcatgcgc
     6721 ctcgtgaagc ttccagccga gcaccgcatt gagcaggtct gcaaaggcgg ccgagtgctc
     6781 cgcaaaccac gcgttgaaga gtgcgaatac cgagcggcgg aacctgttgt cgggactcgt
     6841 cttgaggacg gacgtcgcct gcgcgtagct cagactgatc atgtggccgt cctcgctcgg
     6901 gatcctgagc ttcatcgttg cgacgagatg cgactgctgg gagccgagcg gcagcatgac
     6961 cgcttcaaag agggacgagg actgtgagcc gcgcacggcc agaggcgaga gccacgcttc
     7021 gaagggcgct ctcgcgcgca cctcgtccgg aagctcccgc actgccgaaa gaatcccgcc
     7081 tgaggcctcc tcgagtctga aggagaggcg cttgaccgaa gagagccagg tgcccggcaa
     7141 cgtgtccgcg gcgttcgagc tctgccgcgc acggcagact gtccggagcg agacccccag
     7201 atcgcgcgct tcgtccgcca tgcgcaccag atcgccgagt gcggccgcac ccttcggagg
     7261 cgtcttcgcg agtgcctcga gcgcaacgat cttcgcttcg agacgctcga gcgcctcggc
     7321 gaagccttct tcagaaagga gcggtacgga tctgcccgtc caggcgggcg tcttgttggg
     7381 ggcggggcgg gtcatgcggt cgtataagcg gttcaagaga atttcagggt ttttactggg
     7441 ggtatctaat ggaaaccctt atgcatggtg ctttgggtcg tttgcatgca agtgtaatgc
     7501 cgttccggaa gtggaaaatt gaactttcgg actacgggac aaccctcgtc atcccgaaga
     7561 gcttccctcc gttgccctac gcggctgcat gcgcggcgcg atcatgtgaa ggcttcaaaa
     7621 gaagggtgcc gtccgttcag cgtgcgggga ggcgctccga caatcaacag gagaaacatc
     7681 atgatcggta ttctgatcgg ctgtctcaca ctcgtcgtcg tggggtgggc catcgtcaag
     7741 ggcaagtatg ctccgctcgt gctctttgca agcggcgcct tcatgcttgc gtgcgcagtc
     7801 ttcttcgata cgggcacgtt catgcccaag aaggcgctgc cgacgggcaa cgactatctg
     7861 aacatcatcg agttcatccg ctacatgttc tcgaaccgtc ttgccggcct cggcttcctg
     7921 atcatgacga tggtgggctt tgcctcctac atgacgcaca tcggcgccaa cgacgccttc
     7981 gtgaacatcg cgatcaagcc gctttccatc atcaggaatc cgtacatcct gatcttcgtc
     8041 gccttcatgc tcggcaaggc cgtctcgatg gtgatcacct cggccgtggg cctcggcgtg
     8101 ctctgtctgg cgctcatggg tcccgcgctt gccgcgctcg gcatcaacaa gaaggcgatt
     8161 ggcgccgtgt tcgtcacgtc gggcgcctgc tcgctcgtgc tgctcggcgg ctcgacggcc
     8221 gcctccgcca aggcctgcga cctgtcgatt ctcgactacg tcttcctctt caagattccg
     8281 gccggtattc cgaccgtgct cgccatcggc ctcgcgcacg tcttctggca gcgctggctt
     8341 gaccgtcgcg aaggctggaa ctgcgaggag cacaaaggcg agacgctcgt cttctccgag
     8401 gacgtgaagc gtccgagcac cgccgcaccg aagatctacg ccttcctgcc gttcctcccg
     8461 atggttctgg tggtcgtctt ctcccagtac tgcatctcct cgatcaagct tgacatcacg
     8521 gccctgatgc ttctgtgcgt gatcatcggc atggtctgcg aagtgatccg ctggcgcggc
     8581 aactacgaca agatcgccgc gggcctcaag gtcttcctgc agtcgatggg caaggcgctc
     8641 tctggcgtgg tcgccctcgt gatcgccgcc ggtgtcttcg ccgaaggctt caaggcgctc
     8701 ggcatgctcg acgcgatcgt cgacgtcgcc cacaacgtgg gtgcgggtgc cgtcggcatg
     8761 tccatcctct tcgtcgccat cacgacgctc gtgaccatca tcgcgggctc caacggcgct
     8821 tccttctatc cgctcgtcga aatggtgccc aagatcgccc gcgacatggg cgtcaaccct
     8881 gtgactctcg ttctgccgat gcaccaggct tccacgatcg cgcgtccgct ctcgcccgtc
     8941 gcaggcgtcg tggtcgccat cgccggcatg ctccagatga atccgttcga actcatcaag
     9001 cgcgcctccg tgccctgcat cgtcggtctc atcgtgcatc acgtgagcgt cttctggctc
     9061 gccatttaaa gaagaaagga gcattcaaaa atgacggaat tcaactccgg cgcccccgaa
     9121 tggcagatgg acgacgccta cggcagcttt gcggatccac gctggacgcg cggcgtcgag
     9181 cgcgtgcgcg agctcatcgc cgaaatcgcc cgcctgacga aggacctctc gagcgacgag
     9241 accctgcgcc ttggcctcga ggcctacgag gaggcgctca cgatcgagtc ctccatgtcc
     9301 gccttcgcca agtgcacggg cgcgaaggac acgaccgacg cgtcagcgat ggccgcctcc
     9361 aacttcacgg cgggcctgca tcacgacctc gaggctgctg ccggcccgct cttcgagacg
     9421 ctcgcggcaa agcccgacga ctctcctctc tggagcatga agcccttctc gcactggcgc
     9481 ttcgtcatcc gcgagcgtca ggccgactgg catcgcaggc tgagcgcgac cgacgccgac
     9541 ttctatgcct cgatcgaggt catggccttt gcgccgctcg gttccctgca caagtccctt
     9601 cagaagctcg tcgacttcga cgcgaggaat gccgcaggcg agaccgtgcg ggttcgtgcc
     9661 gccaagctcg tcgccattct caagggcgat cccgaccccg tgctccgcca gagcgccggc
     9721 gaggccatgc aggagtggta cgcccgccac ggcgaactct acgccggcct tctcaacgaa
     9781 ctgcacggca tcaggctcgc gggcttcgag cgtgcgggcg tcgaacccgt gagcgtctcg
     9841 ctcgcccaga accgcatgag ccgcgagtgc ctcgaggcga ttcgcacggc catcttgcgc
     9901 aacacagact ggatccgcga gggcttccgc atgcgcgccc gcgtgctcgg caccccgaag
     9961 atggcctact gggacgtgct tgcgcccgct cctgagacct cgggcggcac cccgcgcctc
    10021 atgccctatg aagaaggcat cggcattgtg aagcgttcac tcggtcgcgt gaatcccgat
    10081 atgactgcct tcgtcgactt gatgctcgag aatcggtgga tcgacgcgaa gccctccgac
    10141 aggaaggtgg ggggcgcttt ctactcgcga ttcaatgaat tcaggatccc gcgcgtcttc
    10201 tcgacctatc tcggctccat cacctcggtc ctgcagcagg gccatgagct tggccacgcc
    10261 ttccactact ggaagatgcg agatctgccg atgatcgaga ccgaattccc gatgacactc
    10321 accgaaaccg cgagcacctt caacgaagcc gtcatccgcc gcgccctcct tgaggaggcc
    10381 gaaggcgccg aacgcttcaa gatgctctgg caggacgtgc gcagcgcggg caacttcctc
    10441 gtcaacacga tggtccgcat ggacttcgag ctcgccttcc tcgaggagcg ccgccgcggc
    10501 gtggtccccg cgggccgctg ctgcgagctc atgcgcgagg tctggacgcg ttggtacggc
    10561 gacgacgtca cgccggacgt gtggctgtgg gcccacaagc ttcactacta caagaccgac
    10621 cagtttatct acaactaccc ctacgccgtc ggctacctga tgagcctcgc gctcatgcgc
    10681 gagtgggagg agaggggaga cgacttctac ggcttctatg tcgcgatgct ccgagacacg
    10741 ggccgcatga ccgtcgacga gatcatccgc cgacacttcg gcgctgacgc cgccgatccc
    10801 ggcttctggg agggtgcaat gcagtctgca cgtcgctcca ccgaggcgct tggggccctc
    10861 atcgaggccc gcggagccga ggcctgaagc cttaaccgct gaatccagaa acggagtgcg
    10921 ccccccgtcg agggcgcgct tcgatcctca ttgaaagacc cattcgacag attgatacga
    10981 cagaaaagga gattcatcat gcaatattcc aagatcgcgc ttgcagtcat gctcaccgct
    11041 gcggccaccg ccgtcagcgc cactgacatc cagatctacg gccgcgttga cgccggcctc
    11101 atgttcgaag atgccgacgg cggcgaacgc tcccttacga tgaagtccgg cggccgcacc
    11161 tccagtcgcc tcggcttcaa catcaaggaa gacctcggca acggaaactt cgtcaaggtc
    11221 tatctggaga acggcttcaa ggttgacgac ggcagcttct ccaccgccga cacgctcttc
    11281 gaccgccgcg ccatcctcgc cgttcagggc gcatggggcg aactcggcct cggccgcgcc
    11341 ggcacggtcc agtccacggc tgctccgtac tccatgggtt ccatcaagtg ggacgtcttc
    11401 ggcacctcct actcgaactc ctcgatcggc acgacgtttg ccaactccag tcgcgtcgac
    11461 aacgccatca actacgtctc gcctgtcatg aacaacctca agttcggcct ctcctactcc
    11521 acgggcgaca aggatgacga cgatgcaaat gaatggcagg acaagggcca cacctttgcc
    11581 gccgccatga actacatcgg cgacaacctc ttcctcggcg tcacgttcgc caacgtcgac
    11641 aacaagcatc ccggcggcac cgccgccgac ggccgcgcct accaggtcgg cggctggtac
    11701 cgtgtcgtgc caaccgtccg catctttgcc ggcgccggct atcagactgc ctggtccacg
    11761 ggcgccaagc tcgccgccac caaggccacg gccatgacgg cggatcagaa gggcggctgg
    11821 gacggctact ctctcgccct cggcttcgag cacctttccg ggccgcacaa gacgttgatc
    11881 ggcgcccagt actttgatgg cgaacttgcc aacaactccg acgtcgactt caagcgtgcc
    11941 gtatacggcg ccgcctatga atattcgttc gccaagaacg tgatcgccta cgtcgcggtc
    12001 aactacagcg acgcttcggg cgccggttcc aaggtcgacg atgcctccgc caaggacacg
    12061 gtccagggct tcctgggcat gagctacctg ttctgaccat gaaccatcag gggggccgcg
    12121 gtctgcacct gaacgctcca tccacagggn nnnnnnnnnc ccctgtcgtc agacagaggc
    12181 cctttcccac gtgtgatcgg cacaggtcag ttcaacggct gaaggtccat agagaggccg
    12241 gctgcgaaag cctcatggcc aactgtgagg tgcaggctga aaaatcacgg cctcctcttg
    12301 tgacactgtc gttgtcccag tcgcccacgg gaaagggttt ttgcttagtc tgaaaggctt
    12361 aagggttcat ggctaaggga agattctgtg ggcagttccg gtggcgatgc gtaaacttct
    12421 gcgcaactgc tacttagcga cacaggacca atttgtcgca gcagttgaat cttttgagga
    12481 gaaacaaatg aacttcaaga ccaagcttgc cgttctcgcg atcggtgccg ccatgggctc
    12541 catggccttc aacgcctccg cctgctcgac ggtcatcgtc ggcaaggacg ccagcgccac
    12601 gggcaacatt ctcgtgggcc acaatgagga caacggcggc cgcattctga ctgctcagca
    12661 ctgggtcccg gctgccactc acaaggccgg cgaaatgatc aagttcgaag aggctgccgc
    12721 ctccattccg caggttgaaa agaccttcgg cttcttctgg tcgcagacct tcgatccgac
    12781 cggcgcctcc ttctccgacg gcttcgtcaa tgaaaacggc gtcacgatcg tcacgaatgc
    12841 ctgcacgggc atttacaagg acgacatcat gccgaccaag gatggcggca tcggctacgg
    12901 catccgccgc ctgatggccg agcgcgccac gagcgcccgt cacgccgtcg acatcgccat
    12961 cgagcttctc ggcaagtacg gctacttctc ggaaggccgc acgtatacga tcgctgaccc
    13021 gaacgaagcc tggcagctgg ccattcatca gggcaatacg tgggtcgcaa ggcgcgtgca
    13081 ggacaatgaa attatctaca ttccgaacaa cttcatgatg gacaaggttg acgcaactga
    13141 caccaagaac gtcatcgttg ctccgggcat gatcgagcgc gccatcaaga acggtcgcta
    13201 caagcccgcc aaggaaggcg tctacagcga cttcaactac cgcgttgccg tcgctccggc
    13261 cgaacgccgc tccgccgact acaactccag ccgcaacaac ctcgcctgga acaagatcat
    13321 cggcaagaac atcacggatc cggaacagtt cccgtactcc gccgtcgcca cgaagaagtg
    13381 gacggttgac gacgtgaagg acctgctgcg cacgcacgaa cacgacatcc aggaacagga
    13441 tgcccagtgg acgcatacga acagcctcgg catctgccgc gccacgacgc atgagtccga
    13501 agtgttcgaa atgaacgcca atccgctcct catcaccggc tatcgcgcac tcgctcgtcc
    13561 gtgcgaatcc ccgtacatcc cgttctatcc gctcgctcgt ccggccgaag gcacggcctt
    13621 catgagctgg gacaaggcca cggccgaaca cttcaagggc acgccggaat acttcggctg
    13681 gcgctctgaa tggccggtca cgacgtttgt cgccgccgcc aatacgtacg acttccagcg
    13741 tcaggaccag aaggatgttc gcgccttcgt cgagaagctt gaagccggct gggaaaagga
    13801 tgttcctgcc gtcaccgctc atgccaagac tcttctcaag gtctccaagg aaaaggccgt
    13861 cgaatatctg catgcctaca acgtccgcat gctgaacgac gcccaggccg ccgttgctga
    13921 aaagcttgaa gaaaaggctc cgtggacgct cgccgtcatg gccgacagca tcaatccgaa
    13981 gagcgacgaa agggttgaag tggttctctt ctcgagcggc aagctcgacg ccacgaaggc
    14041 cgatccgaag cagacctggg gcggtgtggg ccgcgcctcc attggcaaca agatcacgat
    14101 gagtcagaag ctcgcccagc cggtcaaggc ggaagcccgc gacattgacg gcgacggtct
    14161 gaaggacatg gtcttcacgt tcacgcagaa gggcctcgcc cagaacatgc tcgccggcgc
    14221 gaactatgac atctggctcc acacctacgt cgacggcaag cgcgtcgccg ccatggacac
    14281 cgccttcatc gaaaccgaag gctacaaggg tcccgccaag cgcacgcaga acgccgacct
    14341 ctaagtcgtg aatcctgccc gcaggcctcc cggtgaggaa ccggaacctg cggtcgagtc
    14401 cataagctca agaacaatcg agggcgtctt tcgaaaggaa ggcgccctcg atgcgttcag
    14461 tcgtcagtac tcgaagctgt agagcacgtc gaacgcctga ttgacgcccg aggcggcttc
    14521 gaggtaaagg cgcggcatca ggcggtagcg cagcgtgatg gtcgcgagcg agtcgaagat
    14581 gcccacgccg tacttgagct gaaggccggg gaggatgtag cccgacacga cgacctgcga
    14641 gctttcgccc acgcccgccg tgtcgacgcc gagatccttg atgcccaccg cattgccgat
    14701 cgcgccgatg atcctgcccg tctgcgagac gcccagcccc acaagcgccg aagtgatcat
    14761 cgagttgtcg tcgctcgacg tgtcgagccc ctgtccacga agcagatagg agagggtctg
    14821 ctgctgagag agcgcgggtt cggagaatac cgtcaccttc gggcgcatgg cgttgcccgt
    14881 gacgcggatg cccgccgtca cgtcgtcctc gatcgcctcg ggattgcgga tggcctcgat
    14941 gttgagctgc ggacggctgg gcgggcccgc gaagatcacg tgacccgtgc gcacgatgag
    15001 gtcctggccg taggcgtgga aacgcccgtc gtcgacgttg acctggccgt tgaggccgag
    15061 gccgcgcctg gcgtcctgcg tcaccttgag gtcgcccgag agtttcgcct tgaggccaaa
    15121 ggcgtcgatg aagatctgat cgcccatgtg caccacgatg ttgctgtcga tcggaatcgt
    15181 ggcggtcttc gagaccttag gcttcatgtg gcggtcaagg agcacttcgt cagacgagac
    15241 gcccgtggct gtggcgggaa ggtccgccac cgtgatctgc gcggcgggca cctcgatcct
    15301 gccgttgagc gtcacgcgct cggacgaggc cgcaagcgag acgtcgggcg agacggtgac
    15361 cttggcgtac gggggaaccg tgatgaggat gtcgctgcct gaggcatgca cgttcgccgt
    15421 cgggtgcgcc atgtcgcgcc actgcgcgtc gcccgcaagg tcgagcgcgc cctggctcgt
    15481 ctcgaggcgg ccttcaagcg tggacgtgtc gcccgcgaag tcgatccgga tgttcgaggg
    15541 cttcatgacg aggggcacgg cgccttcgga gacccttgcg tccgtgagcg cgatgccgcc
    15601 gttgaggagc ggcttgagca gcgtgcccga gagcgccacg tcgacgttga gcctgccctc
    15661 gaccttttcg tccgaaccga tcagggtgtt gacgatcgac aggtcgccgc cgttgaagag
    15721 gatgcggccg tctagcctgc gggtttcggc gggatcgatc acggcgaccg ttgccttgag
    15781 gtcggcgttg tcgcggatct ttacgtcgag ctcggccgag gcgcgcgact tgtcggtgcg
    15841 cgccttgagg ctgacggtgt cgagatgcac gacgaggggc tcctcgcccg tcgtctgcgt
    15901 gacggtcatg ccgcccgcgt cgacgagcgc atcgatttca ggcaggctgc ccgaggagag
    15961 gtcccacttc gccttggcgg taccgttgac ggtgcctgcc accgtggtga gcggcggcag
    16021 ccagggcttg aggacgtcaa gttcccactt gcgcagtctg agggtggccg agcccttttc
    16081 gcctgcgcgt agcttgtcgt cgatgcagac gtcgcccggc ttgccgaccc agcagtgagg
    16141 ttcgagcacc gcctcggagc ttgaggcctt gtagacgagg cccgcggcct tgtcctgatg
    16201 ccagctgccg atcggcgtcg agacggtgcc gtttgagagc gtgcccttcc agacgaggga
    16261 cttcgtgtcg aaggcgcccg caagcttgag ttcggcgttg acgggctcgc ctgagagatt
    16321 caccgtgagc tcgtgctttc ggtcggtgcc ctcgagccta gcgacgatgt catgaaggac
    16381 gaggctctcg gagagggcgc cgttcgaggc gtgcagcatc atcgagccgc cgacttcgcc
    16441 agagggcttt gcggagatgt cgccgcgaag catcagggcc tcgagcctga cgtcgtcgcc
    16501 gtagcgaagg cccgtggccg tgaggtcggc cttggcgacg ggctcggaga gcgtgccgcc
    16561 gatcgtcacg aagcccgagg cgctgcctgc gagctccttc atgtttctgc ccagatcggg
    16621 cgcgtccacc ttgatgtcgg ccttgatgtt cttgccgtcg agcatggcgc ggcccgtgag
    16681 tttattggcg tcgagcgaga cgctcacgtc ggggacgtag accttcatgg ggctctgcgc
    16741 gatggcggag ccgtgaacgt cgagggcggc gttatcgatc ctgcccttga cgtcgaattc
    16801 gggaagctcg accgaccagc ccttcttgtc gatgcggccc gacgtgcggg agctgcccga
    16861 gaggacggcc ttgacggagg gcgcaaagtg ggagacgtcg aggtttgcga gcttcatgtc
    16921 gccgcgccag cggagcgcgt cgacccatgt gatctcgcca gtgacgcggc ccgtaccgcc
    16981 cgcagtcttc gcggtgagct cgtcgagcgt gagcgaggtg agggtgccgc ggcccttgag
    17041 gccggcggtg gcggacggag tgcccggcgc cttgacgcca agtcctgcat cgagcttcca
    17101 gttgcggacg tcgcccgaca tgcgcaggct gaaggcggag agcgaggtca ggtcggcagg
    17161 gtttgcacct tcgagcggca gcttgaggct gggggagacg agcgtcacgg agaacgggag
    17221 gcccgcagtg gcgggatcgg ccgcaagcag gaacttggcg ggcacgggac ct
//
LOCUS       DBMDTI010000016        17207 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1060_length_17207_cov_2.772536, whole genome shotgun sequence.
ACCESSION   DBMDTI010000016 DBMDTI010000000
VERSION     DBMDTI010000016.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 17207)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17207)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17207
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1060_length_17207_cov_2.772536"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(107..1309)
                     /locus_tag="ACZPB6_01870"
     CDS             complement(107..1309)
                     /locus_tag="ACZPB6_01870"
                     /inference="COORDINATES: protein motif:HMM:NF016895.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR1183258.1"
                     /translation="MQFPNPFKKEKPAEQAAEAPRIEPQLGGEGGEPSPWDVREDPPA
                     FNVVGEEKPLVEPERAAEPVPESVQTPVYSPAAPAASADPVAPAPESAPRGSLFVEPK
                     APQPAAKIEPRLAPRAADAAPETRLVIGEIGSAPSPEAPRRPAPEPEAAAPAGDEEPT
                     GKKRLRMPAFGFMKRKAAPLSDEVEDDVPDEEILVQRQRTRYRLVGAAALMLGVIVVS
                     PLLLDREQDVKPPVSTEIPAVPETARTTLNALPGSSGDVDVTANGAAAEQSTSRANLA
                     NEARQPVKTEPAPKAEAAKPAPAPAARPEPKKAEEKPAKKSSATDGKGFYVQVLATSS
                     ERNAEKLVRELSAKGYPAYKVSVSQKAGTLWRVRVGNYATRDEARGVQGTLVLDGHTG
                     QLIVGNNP"
     gene            complement(1358..2686)
                     /locus_tag="ACZPB6_01875"
     CDS             complement(1358..2686)
                     /locus_tag="ACZPB6_01875"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR1183259.1"
                     /translation="MFDAKTLDAWLTHIEVLHSKPIDLGLDRMKTMVERMGLDFSSRT
                     VITVGGTNGKGSTCAMLESIYRAAGYTTGMHTSPHLIRLNERCVVNGREVDDERLMDA
                     FRAVEKARGDMTLSYFEYTALAILRIFREDELDVVILEIGLGGRLDAINTIDSNAAVI
                     STIGIDHVAFLGNTREAIGLEKAHIYRPGCPAVCADADVPATVRNHVEQIGAKRLFAG
                     EDWTVMVKENGTFTFAQGATLFEDLPEPALAGVNQYRNAGGVLSVIASLMDRVPVTLD
                     DIRTGLRQVRLTARFELITTDPVPTVIDVGHNPQAAEVLLTNVVRSRRPGEHTIAVCG
                     MLADKDMAGVTHILADAFDEWHVASLTGPRAATAAQLHEAMLTAGVEPGRIHEHADVA
                     QALAAARESAVKIAGGAESGAEQAVRIVVFGSFVTVTAALEVFAGEGIAR"
     gene            complement(2791..3666)
                     /gene="accD"
                     /locus_tag="ACZPB6_01880"
     CDS             complement(2791..3666)
                     /gene="accD"
                     /locus_tag="ACZPB6_01880"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR1183260.1"
                     /translation="MSWITHILPKIKREDDNERKPSPIPEGVWTKCPGCEQPLYTEEL
                     RASLQVCPKCGYHHRIGARQRLLAFLDPESAVEIAANVRSVDPLGFVDSKPYPKRIAA
                     AEAASGETDALIAMSGTLRSQPIVATAFEFRFQAGSMGSVVGERFSRAVDEAIRLHAP
                     FVCFAASGGARMQEGLLSLMQMAKTTAAITRLHKAGLPFISVLTDPTMGGVSASFAFM
                     GDVVIAEPKALVGFAGPRVIEQTVREKLPEGFQRAEFLLAKGQIDMIVDRREMRSRIS
                     CLTQHLMRRPPVAGA"
     gene            complement(3785..4591)
                     /gene="truA"
                     /locus_tag="ACZPB6_01885"
     CDS             complement(3785..4591)
                     /gene="truA"
                     /locus_tag="ACZPB6_01885"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR1183261.1"
                     /translation="MKIALGIEYRGTGFNGWQIQPDCPSVQQALENALETFLQVKTPT
                     MCAGRTDTGVHAMQQVVSIETQADRPERSWVRGINTYLPPGVAVRWARRVDDDFHARF
                     SARSRTYEYWIYNDPVRSPILDDLTGWVFRPLDVERMREGAACLVGEHDFTSFRASEC
                     QASGPVRTIRRLELERRGRLIGVRIEANAFLQHMVRNIVGTLIYVGTGREKPEWVEEV
                     LKARSRAAAAPTFSPNGLYLMGVGYEDPSIPAAGPSPFGWEDPEHPSANP"
     gene            complement(4588..6660)
                     /locus_tag="ACZPB6_01890"
     CDS             complement(4588..6660)
                     /locus_tag="ACZPB6_01890"
                     /inference="COORDINATES: protein motif:HMM:NF051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP-related protein"
                     /protein_id="HIR1183262.1"
                     /translation="MFRKSMLAGATALAILGFAVSAQAVSLGDLTVESKPGEPLEALL
                     EINDLDLTISPLLVRVAPPATYLREGVNWPSQVQDLKLARDGSNGTVRLRLYGEQALQ
                     SGSFPLLMEMNAGGTVTVRAYEVQTKDGSFVVTPSAERTTVKGEPRRLSPEPLVVPRE
                     PEAAQDNQEAKKEGKAETKSDSKAESGAAAPTVKTAAAPQKEVSAASAEKHGKIRRKG
                     RYAPNVVKEYVALNGFDASQAFHVQQDMTLWSVALLYWPSYRGATLEQLVIAFRNLNR
                     TAFKEGDPGRLEVRSVLNPPTVEEVLAIDPVAAFHEVHGADTAIPGPTQNLIDAQRLS
                     GEAAAKVADAQDRERTAGKAPEVVAEAGQQALEEEKAEAVREEAEAAEARAEAKAAEE
                     AAEKAAAAEAAAAEKPAESAKPAEPAADAPKVEPVKTEAPARSADAAGSGGTNNLLLW
                     GVGALVLALLAFLGLRSRKREEDPVEEKKPVAVLIQKDVPPTSEAQLKAVSTTVDEAV
                     KNGTTAGAMGAGAMAYAMAQKDETRNSEKALKSEGTGAPADQPWLEPDDDELPPLDEE
                     EKGPVLGASRQAAAVLEGVSLDLDDAVAPAQTSAPAAAPAAATPAPAEQKPAEQPEPA
                     EPETDKERALAKALDAKLKLATSFVSLGAVDEALELLQEVKRRGGDELRHRAEVLEER
                     IKTQTDPK"
     gene            complement(6861..7679)
                     /locus_tag="ACZPB6_01895"
     CDS             complement(6861..7679)
                     /locus_tag="ACZPB6_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIR1183263.1"
                     /translation="MTDNSALRPDALTPKEITEKVLGLGVTKSSMDLRTQLPLTVLAG
                     FYIGLGGLFCTLFMSDSTLPFAVQKFMGGAVFSLGLYLVLVAGAELYTGNTMLAGAAV
                     DGRISWGAAVMNWVRVWLGNFVGALALVTLVYFAHVADTGPMAKGMMSIAVGKLSADW
                     TTIMIKGILCNLLVCLGVWVGYAGRTVVDKAVGVVLPVSAFVALGFEHCVANMYFLPL
                     AFVLKLSGYAPEGVPLDVITIENIVKNLSAATVGNTIAGVVLVLMYWTAFRKRA"
     gene            complement(7981..9336)
                     /gene="rimO"
                     /locus_tag="ACZPB6_01900"
     CDS             complement(7981..9336)
                     /gene="rimO"
                     /locus_tag="ACZPB6_01900"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR1183264.1"
                     /translation="MSHKIPVVGFVSLGCPKALVDTERIVTELRARGYRIGTSYHDAD
                     IVIVNTCGFVNEAVRESLDAITEALAENGRVIVCGCLGGRKEADGSNYVLNHCPKVLG
                     VTGPDSVDDVIAMVETHLPRPHEPWGDLVPACGVRLTPKHYAYLKISEGCNHHCTFCV
                     IPNLRGGLVSRPAGEVIREARNLVDAGVKELLVISQDTAAYGVDRRYALDLRANPPLR
                     THLLDLCRGLGELGVWTRLHYVYPYPSVDEIVPLMAEGLILPYLDVPFQHAHPRVLKA
                     MKRPASAEKNLERIAAWRAVCPDITIRSTFIAGFPGETEEEFEYLLDFLREARLDRVG
                     CFAYSPVEGAQANRLPGALPDEVREERRNRFMEVQAEISAEKLRAKIGTVEEVIIDEY
                     DDEAGLAIGRTKADSPEIDGKCYVETLTPYKPGDFVKVRITGSEEYDLVGTPEDEVQT
                     S"
     gene            complement(9382..10179)
                     /gene="pgeF"
                     /locus_tag="ACZPB6_01905"
     CDS             complement(9382..10179)
                     /gene="pgeF"
                     /locus_tag="ACZPB6_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR1183265.1"
                     /translation="MELTKPGLDILQPAWKDRAPASVDALFTCRDGGVSSGAYGNAEG
                     IMGLNVAPHTGDFGACVRMNRSIVAQLVPSDPVWLRQVHGTEVVDADTAEGEPEADAS
                     VSTKPGVVCTVMTADCLPVLLANREGTVVAAVHAGWKSLAAGIIGKTIRVMRERAPES
                     TGFVAWLAPRIGPEAFEVGPDVREAMLAGLPEADRAFTQGEGDRLMCDLALLAKMALA
                     AEGVSEDDVFDCGLSTYSHPRRFYSFRRDGEKSGRHAAMIWIKPEEA"
     gene            complement(10211..11287)
                     /locus_tag="ACZPB6_01910"
     CDS             complement(10211..11287)
                     /locus_tag="ACZPB6_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1183266.1"
                     /translation="MTSSCENSELSDYNDLPDAGNGADDDTLPSFTVEGFWGERLDKV
                     LATLMPDVSRARLQKLIEEGSVEVNGEVVQKVRQKVTEGDEISLLEEPHLDEALAFTP
                     EEDVEFDVVYEDDAIVVVNKPAGLVVHPGAGNPCGTLLNGLLWRYPELREVPRAGIVH
                     RLDRDTTGLMVVARTLAAQTNLVRQLQERTVKREYWAFTLGSAAPDFVVETPIGRDPR
                     SRTRFKCFPGSTGVRAKPARTRARCVGWSSIEGLPVSWVACRLDTGRTHQIRVHLTSE
                     DLPLIGDQVYRGRAPGLTVKVENELDFHRQALHASRLGLDHPVTGEYMEWFVPPEDDM
                     IDLMDELGFGPWNVPVRVFENERL"
     gene            11286..12071
                     /locus_tag="ACZPB6_01915"
     CDS             11286..12071
                     /locus_tag="ACZPB6_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR1183267.1"
                     /translation="MNFKFPNKRLAARLMAVTAITLATASCSWLESLDKDPTADWSAD
                     KLYTEARAALEESNWTDAKDYYTKLEARYPFGTYAQQAQIELIYANWKDGDAAQAVQG
                     ADRFLQAYPNHANSDYVMYLKALATLNENDGWFAKLAAQDLAERDANAAQEAFDILKE
                     LALRFPDSRYAPEARRRMHQLVLAQAKHELGVAEYYYVRHAYVAAIQHAQRVVSEFQN
                     TPMRDDALEIIANSYDKLEMHDLAADTRRVLELNKGKTVKVRR"
     gene            complement(12461..13777)
                     /locus_tag="ACZPB6_01920"
     CDS             complement(12461..13777)
                     /locus_tag="ACZPB6_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1183268.1"
                     /translation="MPFFSRSALKDGVAMREVWSWAAFDFANSGYTTVVLTAVFNAYF
                     VSVVAGDAPSATFLWTVVIAVSNAVSMLLMPVIGTVADATATKKRWLAVATFMAVGGT
                     LGLMFAGPGTIVWASLMIIVSNVAYNVGESLNSAFLPELAREDAVGKVSGWGWSFGYC
                     GGILTLGLCLGVVLGGEKLGYGMDQTVAGTMAVTAFVFAASALPIFLFLKERSVPRLD
                     AADREAVRSAASESFAELRRTLGSLGDFRDFAWLSVCGFLYQCGVSVVITLSAVYAAA
                     VMGFKTEDTLLMVFLVNITAAVGAFAFGYVQDRIGHKLALAGTLCVWIAMIAVAAFST
                     ERWQFWVAANLAGLAMGSSQSAGRAMVAVFAPSSRLAEFYSFWNMALWLAAIVGPLSY
                     GAITWITGNEHRMAILFTGLFFVLSLLALAPVSMKRGAEVAARLSK"
     gene            complement(13791..14762)
                     /locus_tag="ACZPB6_01925"
     CDS             complement(13791..14762)
                     /locus_tag="ACZPB6_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR1183269.1"
                     /translation="MINQTDVRPLTGYFTHDRIAVPNPLPEAPERPERLDVIERNMLA
                     LGMDALVRVHYAKPAPIESVILAHDLEYVRRLEAASAGDPAALATFDAPDTRVGPDTF
                     DAAMKSAGAVVEAVDELYAGRIKNAFCGVRPPGHHAARAEARGFCYLNNVAIGAYWAM
                     RRHGARRVAIVDFDAHHGDGTEEIVANHPDIRFMSLFQWPLYPHRMMEPTPKNVVVSP
                     VAAGADGAVFTDILENVWLPQLEAFRPDIIFVSAGFDAHVEEQMAQLKVKELDYARIT
                     RRLLDAADVLCEGRLVSVLEGGYAVRSLARSVMAHLNMLVMRGRTAR"
     gene            15069..16184
                     /locus_tag="ACZPB6_01930"
     CDS             15069..16184
                     /locus_tag="ACZPB6_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR1183270.1"
                     /translation="MTTRSRLRMTIYATACAACMAACACAPAADAPANTAKAPQSTVS
                     PSASCYLARPDVVEFLGQISEKHGIPLEWLKDEVAVARYSETAERLMTPKPGIRKPGS
                     DVVKSPFRNYLAYARGFLTQERILRGREFIERNAAIFDEIEMKSGVSRYVITAVIGVE
                     TFYGRNMGRYRVLDSLMTLSFDYTRRAAFFKEELAHFLEFCWRQEVQPVTVLGSFAGA
                     IGYGQFMPSSLDRWGADGDKDGRIDMVESEPDAIASVARFLTAHGWVAGRGLLYPVRA
                     DAEIFDATGSGGIAAHATVEGLRKAGVDTGEHFPLLDDEPVLLVDLPQLDEKGRRTTK
                     WYIGTRNFSSVLRYNRSYFYAAAVAMLANRIAGIDEP"
     gene            16276..16956
                     /locus_tag="ACZPB6_01935"
     CDS             16276..16956
                     /locus_tag="ACZPB6_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR1183271.1"
                     /translation="MSFVFAPPQQAVVPVQDETYSYFPIRRVFGIARNYAEPGAATKD
                     TPFYFMKDCYTVVPVADGECARIPMPPQSQVLKHEIELVACLGKGGRNLTLEQAAEAV
                     WGWCVGIDFTLGDQKLPSGATDFARAKSFERAAPVSHVRPAYRCPMPAPADLWLYVNN
                     QKRQGGCTSNLIVQPLETIVELSRFWELQPGDMIFTGTCGGADKVFPGDHLEGGVNGV
                     GSIKVDII"
BASE COUNT         2899 a         5554 c         5857 g         2897 t
ORIGIN      
        1 tcatcttgaa aaaggctgca gaattgatga agccgttact atagcaggaa tgggggcggg
       61 cgttcgggat atcccgcccc tcaggcgttt acgagccggc gagcgcctag ggattgttgc
      121 cgacgatgag ctggcccgtg tggccgtcga gcacaagcgt accctgaaca ccacgggcct
      181 cgtcgcgggt cgcgtagttg cccacgcgca cgcgccagag cgtgcccgcc ttctggctca
      241 ccgaaacctt gtaggccgga tagcccttgg cggaaagctc cctgacgagc ttttcggcat
      301 tgcgctcgct gctcgtcgcg agcacctgaa cgtagaagcc cttgccgtcc gtggcggagc
      361 tcttcttcgc aggcttttcc tcggccttct tgggctcggg cctggcggcg ggcgcaggcg
      421 cgggtttggc ggcctcggcc ttgggcgcgg gctccgtctt cacgggctgg cgagcctcgt
      481 tggcaaggtt ggctctggag gtggactgct ccgcggcggc gccgttagcc gtcacgtcga
      541 cgtcgcccga actgccgggc agggcgttga gcgtcgtcct cgccgtttcg ggcacggccg
      601 gaatctcggt cgagacgggc ggcttgacgt cctgctccct gtcgagcaaa agcggcgaga
      661 caacgatgac gccgagcatc agggcggcgg cacccacgag cctgtagcgg gtgcgctggc
      721 gctggacaag gatctcctcg tcgggcacgt cgtcctcgac ctcgtcggaa agcggcgcgg
      781 ccttcctctt catgaatccg aacgcaggca tcctgagacg cttcttcccc gtcggctcct
      841 cgtcccctgc gggcgcggcg gcctcgggtt cgggcgcggg acgccgcggc gcctcgggcg
      901 aaggcgcact gccgatttcg ccgatcacga gacgggtctc gggcgcggca tccgccgcgc
      961 ggggcgcgag gcgcggctcg atcttggccg caggctgcgg agccttcggc tcgacgaaca
     1021 gggagccgcg cggcgcagac tcgggagcgg gtgcaacagg gtccgcagat gctgcgggcg
     1081 ccgcgggaga gtaaacggga gtctgaacgg actccggaac gggctcggca gcgcgttcgg
     1141 gctcgacgag cggcttttcc tcgcccacaa cgttgaaggc cggcggatcc tcgcggacat
     1201 cccaggggga gggctctccg ccttcgcctc cgagctgagg ctcgatgcgg ggcgcttcgg
     1261 cggcctgttc ggccggcttc tcttttttga acggattggg aaactgcatg gcgtgtggcc
     1321 cctcaggtgg tgttgggtct tccggtcctt cgctgcatca gcgagcgatg ccttcccctg
     1381 cgaagacttc gagcgcggcg gtcacggtga cgaaggagcc gaatacgacg attctaacag
     1441 cctgttccgc gcccgattct gcgccgcctg caatttttac ggcgctctcg cgcgcggcgg
     1501 cgagcgcctg cgcaacgtcg gcgtgctcgt ggatgcggcc gggctcgacg cccgccgtaa
     1561 gcatagcttc gtgcagctgt gctgcggtgg cggcgcgcgg ccccgtgagg gaggcgacgt
     1621 gccactcgtc gaaggcgtcg gcaagaatgt gcgtcacgcc cgccatgtcc ttgtcggcga
     1681 gcatgccgca gacggcgatc gtgtgttcgc cgggcctgcg gcttcggacg acgttggtga
     1741 gaagcacctc ggccgcctgc ggattgtgac cgacgtcgat gacggtgggc acgggatcgg
     1801 tggtgatgag ttcgaagcgg gccgtcaggc ggacctggcg cagacccgtg cggatgtcgt
     1861 cgagcgtgac gggcacgcgg tccatcaggg aggcgatgac ggagaggacg ccgcccgcat
     1921 tgcggtactg gttgacgccg gcgagcgcgg gctcgggcag gtcctcgaag agcgtcgcgc
     1981 cctgagcgaa cgtaaacgtg ccgttctcct tcaccatcac cgtccagtcc tcgcccgcga
     2041 agagtcgctt cgcgccgatc tgctcgacat gattcctcac cgtggcgggc acgtcggcgt
     2101 cggcgcagac ggcggggcag cccggacggt agatgtgagc cttctcgagg ccgatcgcct
     2161 cgcgcgtgtt gccgaggaag gcgacgtggt cgatgccgat cgtcgagatg acggcggcgt
     2221 tggagtcgat cgtgttgatg gcgtcaagac ggccgccgag cccgatctcg aggatcacga
     2281 cgtcgagctc gtcctcgcgg aagatcctga ggatggcgag cgccgtgtat tcgaagtagg
     2341 agagcgtcat gtcgccgcgg gccttctcga ccgcgcggaa ggcgtccatg aggcgttcgt
     2401 cgtctacttc gcgcccgttg actacgcagc gctcgttgag gcggatgagg tgcggcgagg
     2461 tgtgcatgcc ggtcgtgtag cccgccgcgc ggtagatgct ctcgagcatc gcgcaggtcg
     2521 agcccttgcc gttcgtgccg cccacggtaa tgacggttcg ggacgagaag tcgagcccca
     2581 tgcgctcgac catggtcttc atgcgatcga gtccgaggtc gatgggcttc gagtgcagca
     2641 cttcgatgtg cgtgagccac gcgtcaaggg tcttggcatc aaacatgaaa gggaatctcc
     2701 agtctggaaa atggaaaagc cgtccgaacg atgcgtcctg cgctcgtctc gggcggctcg
     2761 aaaatgcgtg cgggtcggga gagccgtgcg tcaggcgccc gccacgggcg gcctgcgcat
     2821 caggtgttgc gtgaggcagg agatgcgcga gcgcatctcg cggcggtcga cgatcatgtc
     2881 gatctggccc ttggcaagca ggaactcggc gcgctggaag ccctcgggga gcttttcacg
     2941 cacggtctgc tcgatgacgc gcgggcccgc aaagcccacg agggccttgg gttcggcgat
     3001 cacgacgtcg cccatgaaag cgaacgaggc ggagacgccg cccatcgtcg ggtcggtgag
     3061 caccgaaatg aaggggagcc cggccttgtg cagacgcgtg atggcggccg tcgtcttggc
     3121 catctgcatg agggagagaa ggccctcctg catgcgggcg ccgcccgaag ccgcgaagca
     3181 cacgaagggc gcgtgcaggc ggatcgcctc gtcgacggcg cgggagaagc gctcgccgac
     3241 gacgctgccc atggagcccg cctggaagcg gaactcaaag gccgtcgcga cgatcggctg
     3301 gctgcgcaac gtacccgaca tcgcgatgag cgcgtcggtt tcgcccgagg ccgcctcggc
     3361 cgcggcgatg cgcttcggat acggcttgct gtcgacgaag ccgagcgggt ccacgctgcg
     3421 cacgttggcg gcgatttcga cggcgctctc gggatcaagg aaggcgagaa ggcgctggcg
     3481 ggcgccgatg cggtgatggt agccgcactt cgggcagacc tgaaggctgg cgcggagctc
     3541 ttccgtgtaa agcggctgct cgcagcccgg acacttggtc cagacgcctt cgggaatcgg
     3601 actcggcttg cgctcgttgt cgtcctcgcg cttgatcttg gggagaatgt gcgtaatcca
     3661 gctcataagc tcttcatttc aggttcgtcc gctccctgcc gtgcgacggc ggggacggac
     3721 cgtttcaaaa ctgcgccaat tatatcgccc gggaggaatc ccaaaggcgc gccccgcatc
     3781 ggcttcacgg attagcggaa ggatgctcgg gatcctccca gccgaaggga ctcggccccg
     3841 cggcggggat ggacggatcc tcgtagccca cgcccatcag gtataggccg ttcgggctga
     3901 aggtgggcgc ggccgcggcg cggctgcgcg ccttgaggac ctcctcgacc cactcgggct
     3961 tctcgcggcc cgtgcccacg tagatgagcg tgcccacgat gttgcgcacc atgtgctgca
     4021 ggaaggcatt ggcctcgatg cgcacgccga tgaggcggcc gcggcgctca agctcgaggc
     4081 ggcggatcgt acgcacggga cccgaggcct ggcactcgga ggcgcggaag ctcgtgaagt
     4141 cgtgctcgcc cacgaggcag gcggcaccct cgcgcatgcg ctcgacgtcg agcgggcgga
     4201 agacccagcc cgtcagatcg tcgagaatgg gagagcgcac gggatcgttg taaatccagt
     4261 actcgtaggt gcgcgagcgt gcggagaagc gcgcgtggaa gtcgtcgtcg acgcgccttg
     4321 cccagcgcac ggcgacgccg ggcggcaggt acgtgttgat gccgcgcacc cagctgcgct
     4381 cggggcggtc ggcctgcgtc tcaatgctca ccacctgctg catggcgtgc acgcccgtat
     4441 ccgtgcgacc cgcgcacatc gtcggcgtct tcacctgcag gaaggtctca agcgcattct
     4501 caagcgcctg ctgcacggac gggcagtcgg gctggatctg ccaaccgttg aagcccgtgc
     4561 cgcgatattc aatgccaagc gctatcttca cttcggatcc gtctgagtct tgatgcgttc
     4621 ttcaaggact tcggcgcgat ggcgaagctc gtcgccgccg cggcgcttca cttcctggag
     4681 aagctcgagg gcctcgtcca cggcgccgag gctcacgaag ctcgtggcga gcttgagctt
     4741 ggcgtcaagc gccttggcaa gcgcacgttc cttgtcggtt tcgggttccg cgggctcggg
     4801 ctgttctgcg ggcttctgct ccgcgggagc aggcgtggca gctgcaggag cggcggcagg
     4861 ggcggaagtc tgagcggggg cgaccgcatc gtcaaggtcg agcgagacgc cctcgagaac
     4921 ggcggcggcc tgcctggagg cgccgagcac ggggcccttc tcctcttcgt caagcggcgg
     4981 cagctcgtcg tcatcgggct cgagccacgg ctggtcggcc ggggccccgg ttccttcgga
     5041 cttcagagcc ttttcggaat tccgggtctc gtccttctgc gccatggcgt aggccatcgc
     5101 gcccgcgccc atggcgccgg cggtcgtgcc gttcttcacg gcttcgtcaa cggtcgtgct
     5161 gacggccttg agctgggctt cggaggttgg cgggacatcc ttctgaatca ggacggcaac
     5221 gggcttcttc tcctcgacgg gatcttcctc acgcttgcgc gagcgaaggc cgaggaaggc
     5281 gagaagcgcc agaacgaggg cgccgacgcc ccagaggagc aagttgttcg tgccgccgga
     5341 gcctgcggca tcagccgacc gggcgggcgc ctcggtcttg acgggctcga ccttgggcgc
     5401 atcggcggcg ggctctgccg gcttggcgga ctcggcgggc ttttcagcgg ccgcagcttc
     5461 tgcggctgca gccttctccg cggcctcttc ggcggccttt gcttcggcac gcgcctcagc
     5521 ggcttcggcc tcttccctga cggcctcggc cttctcctcc tcaagggcct gctggcccgc
     5581 ctcggcgacg acctcggggg ccttgcctgc ggtgcgctcg cggtcctgag catcggccac
     5641 cttcgcggcg gcctcgccgg agagccgttg ggcgtcaatc agattctgcg tggggccggg
     5701 aatggccgtg tccgcaccgt gcacttcgtg gaaggcggcg acgggatcaa tggcgaggac
     5761 ctcctcgacc gtcggcggat tgaggacgga acggacctcg agcctgcccg gatcgccttc
     5821 cttgaaggcg gtgcggttga ggtttcggaa tgcgatgacg agctgctcga gcgtggcgcc
     5881 gcggtagctc ggccagtaga gcaaggcgac ggaccagagg gtcatgtcct gctgcacgtg
     5941 gaaggcctgc gaagcgtcga aaccgttgag cgccacgtat tccttgacga cgttgggcgc
     6001 atagcggcct ttgcggcgga tcttcccatg cttctcggca gaggcggcgg agacttcctt
     6061 ctgcggggcg gcagccgtct tcacggtggg cgctgcggcc ccggattcgg ccttggagtc
     6121 ggacttcgtc tcggcctttc cctccttctt cgcctcctga ttgtcctgag cggcctcagg
     6181 ctctctgggc acgacaagcg gctcggggga gaggcggcgg ggctcgccct tgacggtggt
     6241 gcgttcggcc gaaggtgtca cgacgaatga gccgtccttc gtctgcactt cataggcgcg
     6301 gacggtgacg gtgccgcctg cgttcatctc catgagaagc gggaagctgc ctgactgaag
     6361 ggcctgctcg ccgtagaggc gcaggcgcac ggtgccgttg ctgccgtcgc gcgcaagctt
     6421 gaggtcctga acctgggaag gccagttcac gccttcgcgc aggtaggtgg cgggaggcgc
     6481 cacgcgaacg agtagcggag agatcgtcag atcgaggtcg ttgatctcca acagggcctc
     6541 aaggggctca cccggcttgc tttcgacagt caggtcgccg agcgagacgg cctgcgccga
     6601 aacggcgaag ccgaggattg caagcgccgt tgcgcccgcc agcatgcttt ttctgaacat
     6661 cagtcaattc cgaacaggcc cgcgaagccc gtcgggcgtt tcgcggagtc atgaataacc
     6721 tgttgataat accgcatgga accgtgcgag cggaaatacg cattttccgc agggcgagcc
     6781 cgtgcggcac ccgcttcagg cctgaaaaaa gccgccgtgc ccgcgggcga cggcggcttc
     6841 gggaagccgt ttcaggatac tcaggcgcgc tttctgaagg cagtccagta catgaggacg
     6901 agaacgacgc ccgcgatggt gttgccgacc gtggcggcag agaggttctt gacgatgttc
     6961 tcgatcgtga tcacgtcgag cggcacgccc tcgggcgcgt agcccgaaag cttgaggacg
     7021 aaggcgagcg gcaggaagta catgttcgcc acgcagtgct cgaagcccag cgccacgaag
     7081 gcggagacgg gaagcacgac gcccacggcc ttgtcgacga cggtgcggcc cgcatagccc
     7141 acccagacgc cgaggcacac gagcaggttg cagaggatgc ccttgatcat gatcgtcgtc
     7201 cagtcggcgg aaagcttgcc gacggcgatg ctcatcatgc ctttggccat gggacccgtg
     7261 tcggccacgt gcgcgaagta gacgagcgtg acgagcgcga gggccccgac gaagttgccg
     7321 agccagacgc gcacccagtt catgaccgcc gcgccccagc tgatgcggcc gtcgacggcc
     7381 gcacccgcga gcatggtatt gcccgtgtaa agctcggcac ctgcgacgag cacgaggtag
     7441 aggccgagcg agaagacggc accgcccatg aacttctgca ctgcgaaggg aagcgtggag
     7501 tcgctcatga agagcgtgca gaagagcccg ccgagcccga tgtagaagcc ggcgagtacg
     7561 gtgaggggca gctgggtgcg cagatccatg ctgcttttgg ttaccccgag gccgagcacc
     7621 ttttcggtga tttccttcgg ggtgagtgcg tcgggacgca gtgcgctgtt gtcggtcatg
     7681 ggaagctccg gaatgtttga ataactgtgg gcgaggcccg tttggaaagc gtcctcggac
     7741 gaggcgtcgt tttccaaacc ggccgcagct tctggctcgg aggagcctga cctgaaacgg
     7801 cggaatcaaa ggaacggaca ttgtattgaa gttttcgcgc cccttcgggc gactacttcc
     7861 aattttcgcc gaaagatgta ttcacttttt tccgcgcccg tcttccgaaa acgacgaagg
     7921 cttcgagagc gcctgtttcc tgggctctcg aagccttctg cgtgatgcgt ttcggacgaa
     7981 tcagctcgtc tgaacctcgt cttcgggcgt gcccacgagg tcgtactcct cgctgcccgt
     8041 gatgcggacc ttcacgaagt cgcccggctt gtagggcgtg agggtctcga catagcactt
     8101 gccgtcgatc tcgggagagt cggccttcgt gcgaccgatg gcaagacctg cctcgtcgtc
     8161 gtactcgtcg atgatgacct cctcgacggt gccgatcttg gcgcggagct tctcggccga
     8221 gatctcggcc tgaacctcca tgaagcggtt gcggcgctcc tcgcgaacct cgtcgggcag
     8281 cgcaccgggg agccggttgg cctgggcgcc ctcgacgggc gagtaggcga agcagcccac
     8341 gcggtcaagc ctggcctcgc gcaggaagtc gagcaggtac tcaaactcct cttcggtctc
     8401 gccggggaag cccgcgatga aggtcgagcg gatcgtgatg tcggggcaga ccgcgcgcca
     8461 ggcggcaatg cgctcgaggt tcttctcggc gctcgcgggg cgcttcatgg ccttaagcac
     8521 gcgcgggtgc gcatgctgga acggcacgtc gagatacggc aggatcaggc cctcggccat
     8581 gagcggcacg atctcgtcga cggacggata cggatagacg tagtgcaggc gcgtccagac
     8641 gccgagctcg cccaggcccc tgcagaggtc gagcagatgc gtgcgcagcg gcggattggc
     8701 gcgcagatcg agcgcgtagc ggcggtcgac gccgtaggcg gccgtgtcct ggctgatgac
     8761 gaggagctcc ttgacgcccg cgtcgacgag gttgcgggcc tcgcggatca cttcgcccgc
     8821 aggacggctc acgaggccgc cgcgcaggtt cgggatgacg cagaacgtgc agtggtgatt
     8881 gcacccctcg ctaatcttga ggtaggcgta gtgcttgggc gtgaggcgca cgccgcaggc
     8941 gggcaccaga tcgccccagg gctcgtgggg acgcggcaga tgggtctcga ccatcgcgat
     9001 gacgtcgtcg acggagtcgg ggcccgtgac gccgagcacc ttcgggcagt ggttcaacac
     9061 gtagttgctg ccgtcggcct ccttgcggcc gccgaggcag ccgcagacga tgacgcggcc
     9121 gttttcggcg agcgcctcgg tgatggcgtc aaggctttcc ctgacggcct cgttgacgaa
     9181 gccgcaggtg ttgacgatca cgatgtcggc gtcgtggtag ctcgtgccga tgcggtagcc
     9241 tcgggcgcgg agctcggtga cgatccgctc cgtatcgacg agggccttgg ggcagccgag
     9301 ggagacgaag ccgacgacgg gaatcttgtg ggacatggga gtctgaacct gtaaaagcgg
     9361 ttgttcggcg ggaccgggag atcaggcctc ttcgggcttg atccagatca tcgcggcgtg
     9421 gcggccggac ttctcgccgt cacggcggaa gctgtagaag cggcgcggat gcgaataggt
     9481 ggagaggccg cagtcgaaga cgtcgtcctc ggagacgccc tcggcggcga gcgccatttt
     9541 tgcgagcaaa gcgaggtcgc acatcaggcg gtcgccttcg ccctgcgtga aggcgcggtc
     9601 ggcttcgggg agaccggcga gcatcgcctc gcgaacgtcc gggccgacct cgaaggcctc
     9661 gggaccgatg cgcggggcga gccaggcgac gaaacccgtg ctttcggggg cgcgttcgcg
     9721 catgacgcgg atcgtcttcc cgatgatgcc ggcggcaagg ctcttccagc ccgcatggac
     9781 ggcggcgacg accgtgccct cacggttggc gaggagcacg ggcaggcagt cggcggtcat
     9841 gaccgtgcag acgacgcccg gcttcgtgga gacggaggca tcggcctcgg gttcgccttc
     9901 ggccgtgtcg gcgtcgacga cctcggtgcc gtgcacctgc ctgagccaca cgggatcgga
     9961 cggcacgagc tgcgcgacga tggagcggtt catgcgcaca caggcgccga agtcgcccgt
    10021 atgcggggcg acgttgaggc ccatgatgcc ttcggcgttg ccgtaggcgc cgctcgagac
    10081 gccgccgtcg cggcacgtga agagcgcgtc gaccgaagcc ggggcgcggt ccttccaggc
    10141 gggctgcaga atgtcgagtc cgggtttcgt cagttccatg accttcctcc tatatcttgt
    10201 ggtgcctgct tcagaggcgt tcgttctcaa agaccctgac cgggacgttc caggggccga
    10261 agccgagttc gtccatcagg tcgatcatgt cgtcctcggg cggcacgaac cactccatgt
    10321 actcgcccgt gaccggatgg tcgagaccga ggcgggaggc atgcagcgcc tggcggtgga
    10381 agtcgagctc gttctcgacc ttgaccgtga ggccgggcgc gcggccgcgg tagacctggt
    10441 cgccgatgag cggcaggtcc tcgctcgtga ggtggacgcg aatctggtgc gtgcggcccg
    10501 tgtcgaggcg gcaggcgacc caggagacgg gcaggccctc gatggagctc cagcccacgc
    10561 agcgggcacg agtgcgtgcc ggcttggcgc gcacgcccgt gctgccgggg aagcacttga
    10621 agcgggtgcg gctgcgcgga tcgcggccga tcggcgtctc gacgacgaag tcgggcgcgg
    10681 cggagccgag cgtgaaggcc cagtactcgc gcttgacagt gcgctcctga agctggcgca
    10741 cgaggttcgt ctgggcggca agcgtgcggg cgacaaccat gaggccggtc gtgtcgcggt
    10801 cgaggcggtg gacgatgccc gcacggggca cctcgcggag ctcgggatag cgccacagca
    10861 ggccgttgag cagcgtgccg caggggttgc ccgcgcccgg atgcacgacg agaccggcgg
    10921 gcttgttgac gacgacgatc gcatcgtcct cgtagaccac gtcgaactcc acgtcctcct
    10981 cgggcgtgaa ggcgagcgcc tcgtcaagat ggggctcctc gagcagcgag atctcgtcgc
    11041 cctcggtgac cttctggcgc accttctgga cgacctcgcc gttcacctcg acgcttccct
    11101 cctcgatgag cttctgcaga cgagctcggg aaacatcggg catcagggtg gcgagaacct
    11161 tgtcaaggcg ttctccccag aacccttcca ccgtgaaact tggtaaagtg tcatcatcgg
    11221 cgccgttccc ggcatcgggg aggtcgttat aatccgaaag ttcagaattt tcgcaactgc
    11281 ttgtcatgaa tttcaagttt cctaataagc gcctcgccgc gaggctgatg gccgtgacgg
    11341 ccatcacgct ggcgaccgcc tcctgctcct ggctcgaatc cctcgacaag gatccgacgg
    11401 cggactggtc ggccgacaaa ttgtataccg aggcccgagc cgctcttgaa gagagcaact
    11461 ggaccgatgc aaaagactat tacaccaagc tcgaggctcg ctatccgttt ggaacctatg
    11521 cacagcaggc ccagatcgag ctgatctacg cgaactggaa ggacggcgac gccgcccagg
    11581 ccgttcaggg tgcggaccgc ttcctgcagg cctatccgaa ccacgccaac agcgactacg
    11641 tcatgtacct gaaggcgctt gcgaccctca atgaaaacga cggctggttc gcgaagctcg
    11701 ccgcgcagga ccttgccgag cgcgacgcca atgccgccca agaagccttc gacatcctca
    11761 aggagctcgc cctgcgcttc cccgacagcc gctacgcgcc cgaagcgcgc cgccgcatgc
    11821 accagctcgt gctggctcag gcaaagcatg aacttggagt tgcagaatac tactacgttc
    11881 gacacgctta tgtcgcggcc attcagcacg cgcagcgagt cgtgagcgaa ttccagaaca
    11941 cgcccatgcg cgacgacgct cttgagatca tcgcgaactc ctacgacaag cttgaaatgc
    12001 acgacctcgc cgccgacacg cgcagggtcc tcgagctcaa caagggcaag accgtcaagg
    12061 tccgccgctg agtcgggctc tgatttccgc tcgcaaagag gaggcttcct gaaggaggtc
    12121 tccttttttg tttaccaccc tccttttctt gatcaggatc aagtcaagga caatctcctc
    12181 gaaaaaactc cacaaacgat attgagaacc attctcaatt caggcacaat acattcaacg
    12241 acagcgaaac gccgccgcct ccatccaagg acaacggggg tcgtccggag cggacggcga
    12301 ggaaaggaac caagcgcctt cccgcaaagc ggggcaatct ccacccgcat gctgttgggg
    12361 gtctgagaag ggccggatgc gtgcaacgcg catccggccc tgtttttatc ctgctctcgt
    12421 gcagaaggca ccctgctgtg agcgccctgc tgcgggcgtc ctactttgag agccttgcgg
    12481 ccacctcggc gccgcgcttc atgctcacag gcgcgagcgc cagaagggag agcacgaaga
    12541 agaggcccgt gaaaaggatg gccatcctgt gttcgttgcc ggtgatccag gtgatggcgc
    12601 cgtaggaaag cgggccgacg atggcagcaa gccacagcgc catgttccag aagctgtaga
    12661 actcggccag gcgcgagctc ggcgcgaaga ccgcgaccat ggctcggcct gcggactggg
    12721 acgagcccat ggcgaggccc gcgagattgg cggcgaccca gaactgccag cgctcggtcg
    12781 agaaggcggc gacggcgatc atcgcgatcc agacgcagag cgtgcccgcg agcgcgagct
    12841 tgtggccgat gcggtcctgc acgtagccga aggcgaaagc gccgacggcg gccgtgatgt
    12901 tcacgaggaa gaccatgagg agcgtgtcct cggtcttgaa ccccatgacg gcggcggcat
    12961 agacggccga gagcgtgatg acgacggaga cgccgcactg atagaggaac ccgcagacgg
    13021 aaagccaggc gaagtcgcgg aagtcgccga gcgagccgag cgtgcggcgc agctccgcaa
    13081 agctctccga ggcggcgctc ctgacggcct cgcggtccgc ggcgtcaaga cgcggcacgc
    13141 tcctctcctt gaggaagagg aagatcggaa gcgcggaggc cgcgaagacg aaagccgtca
    13201 cggccatcgt gcccgccacg gtctggtcca tcccgtagcc gagcttctcg ccgccgagca
    13261 ccacgcccag acagaggccg agcgtaagga tgccgccgca gtagccgaag ctccagcccc
    13321 agcccgaaac cttgcccacg gcgtcctcac gggcgagctc gggcaggaag gccgagttga
    13381 gggactcgcc cacgttgtag gcgacgttgc tcacgatgat catgagggag gcccagacga
    13441 tcgtgcccgg tcccgcgaac atgaggccga gcgtgccgcc cacggccatg aaggtcgcga
    13501 cggcgagcca gcgcttcttc gtggcggtcg cgtcggccac cgtgccgatg acgggcatca
    13561 gcagcatgct caccgcattg ctcaccgcga tgacgacggt ccagaggaac gtcgccgagg
    13621 gggcgtcgcc cgccaccacg ctcacgaagt aggcgttgaa gacggccgtg agcaccacgg
    13681 tcgtgtagcc cgaattggca aagtcgaatg cggcccagga ccagacctcg cgcatcgcga
    13741 cgccgtcctt gagagcggac cgggagaaaa acggcatgtg gttctcctcc ttatcgcgcg
    13801 gttcgtccgc gcatgaccag catgttgaga tgcgccatca cgcttcgcgc aaggcttctg
    13861 acggcgtaac cgccctcgag cacgctcacg agacgcccct cgcagaggac gtcggccgca
    13921 tcgagcagcc tgcgcgtgat gcgcgcgtag tcgagctcct tcaccttgag ctgtgccatc
    13981 tgctcctcga cgtgggcgtc gaagcccgcc gagacgaaga tgatgtcggg cctgaaggcc
    14041 tcgagctgcg gcagccagac gttctcgagg atgtcggtga agaccgcgcc gtcggcgcct
    14101 gcggcaacgg gactgacgac gacgttcttg ggcgtgggct ccatcatgcg gtgcggatag
    14161 agcggccact ggaaaagcga catgaagcgg atgtcgggat ggttcgcgac gatctcctcg
    14221 gtgccgtctc cgtggtgcgc gtcaaagtcg acgatcgcga cgcgtctcgc gccgtggcgg
    14281 cgcatcgccc agtaggcgcc gatcgccacg ttgttgaggt agcagaagcc gcgagcctcg
    14341 gcgcgcgccg catggtgtcc cggcgggcgc acgccgcaga aggcgttctt gatgcggccc
    14401 gcatagagct cgtcgaccgc ctcgaccacc gcgcccgcac tcttcattgc ggcgtcgaag
    14461 gtgtcgggac cgacgcgcgt gtcgggcgcg tcgaaggtcg caagcgcggc gggatcgccc
    14521 gccgaagcgg cctcgaggcg cctgacgtac tcgaggtcgt gcgcgaggat cacggactcg
    14581 atcggcgcgg gcttcgcata gtgaaccctg acgagggcgt ccatcccgag ggcgagcatg
    14641 ttgcgctcga tgacgtcgag cctctcgggg cgctcggggg cctcgggaag cgggttgggc
    14701 acggcgatgc ggtcgtgcgt gaagtagccc gtgagcgggc gaacgtccgt ttgattgatc
    14761 atctgcaatg gcctgttctt gttattggcg gcgttcatgc gggctgcccg cccgcgcagt
    14821 ccgcgcgggc ctgcgggagg cgccccatga cggaaagcgt accacaggcg gcccacccga
    14881 aaaagagacc gctcgggaac gggccccgca gcttcgacgc ctccgaggcg cactgcacgt
    14941 gcgacgcctc tcccgcaggc atcccgccgt caatttgtgc catcctgaaa cacggccccg
    15001 cgccggtgag gcccgtcggg cgagccttgc tacaatgcct caggtcaccc atccaccgca
    15061 caaccttcat gacgacacga tcccgactcc gcatgacgat ctacgcgacc gcctgcgccg
    15121 cctgcatggc ggcctgcgcc tgcgcgcccg ccgcggatgc gcccgccaat acggcgaagg
    15181 cgccccagtc gaccgtctcg ccctccgcat cctgctatct cgcccggccc gacgtcgtcg
    15241 aatttctggg ccagatctcg gaaaagcacg gaattccgct cgagtggctc aaggacgagg
    15301 tggccgtcgc ccgctactcg gaaacggccg agcgcctgat gacgcccaag cccggcatca
    15361 gaaagcccgg ctccgacgtc gtcaagtcgc ccttcaggaa ctacctcgcc tacgcacgcg
    15421 gcttcctcac gcaggaacgc atcctgcgcg ggcgcgagtt catcgagcgc aacgccgcca
    15481 tcttcgacga aattgaaatg aagagcggcg tctcccgcta cgtcatcacc gccgtcatcg
    15541 gcgtcgagac cttctacggc cgcaacatgg gccgctaccg cgtgctcgac agcctgatga
    15601 cgctctcctt cgactacacg cgccgcgccg ccttcttcaa ggaggagctc gcgcacttcc
    15661 tcgagttctg ctggcgtcag gaagtccagc ccgtcaccgt gctcggtagc ttcgcgggcg
    15721 ccatcggcta cggccagttc atgccgagct cgctcgaccg ctggggcgcc gacggcgaca
    15781 aggacggcag gatcgacatg gtcgagagcg agcccgacgc catcgcctcc gtcgccagat
    15841 tcctcacggc ccacggctgg gtcgcgggcc gcggactcct ctaccccgtc agggccgacg
    15901 ccgagatctt cgacgcgacg ggctcgggcg gcatcgccgc acacgccacc gtcgagggac
    15961 tgcgcaaggc gggcgtcgac acgggagagc acttcccgct cctcgacgac gagcccgtcc
    16021 tgctcgtcga cctgccgcag ctcgacgaaa agggccggcg caccacgaag tggtacatcg
    16081 gcacccgcaa cttctcctcg gtcctgcgct acaaccgcag ctacttctat gcggccgccg
    16141 tcgccatgct cgccaaccgc atcgcgggca tcgacgagcc ctgacgcctc ctgcgcgcac
    16201 ccgctcgcgc ccgaggcgaa gcatttgcta tatttccctc gatgattttt tccatctcct
    16261 ttccggaggc ccttcatgtc ttttgtcttt gccccgcccc agcaggccgt cgtgcccgtt
    16321 caggacgaaa cctactccta cttcccgatc cgccgcgtct tcggcatcgc caggaactac
    16381 gccgaaccgg gcgccgcgac gaaagacacg cccttctact tcatgaagga ctgctacacg
    16441 gtggtccccg tggccgacgg cgaatgcgcc cgaatcccga tgccgccgca gagtcaggtg
    16501 ctcaagcacg agatcgagct cgtcgcctgc ctcggcaagg gcggccgcaa cctcacgctc
    16561 gaacaggcgg ccgaggccgt ctggggctgg tgcgtcggca tcgacttcac gctcggtgac
    16621 cagaagctgc cgtcgggcgc caccgacttc gcgcgcgcca agtccttcga gcgcgccgcg
    16681 cccgtgagcc acgtgcgccc cgcctaccgc tgcccgatgc ccgcccccgc cgacctctgg
    16741 ctctacgtca acaaccagaa gcgacagggc ggctgcacgt ccaacctgat cgtccagccc
    16801 cttgagacga tcgtcgagct ctccagattc tgggagctcc agcccggcga catgatcttc
    16861 acgggcacct gcggcggcgc cgacaaagtg ttcccgggcg accaccttga gggcggcgtc
    16921 aacggcgtcg gctccatcaa ggtcgacatc atctgacgcc gaaacggaaa cgttcactta
    16981 cgtttgggtg gcgcgtccgc ggacgcgcca cccttttgcg tcatggatca agcagtcaaa
    17041 agttgctaag atcatggatt gaccgcgaga cgccctgaga aacgggcgtt ccgcttcaaa
    17101 aaagggaccg ccgccacggc cttcgcgccg ttgcgcagac gcagggagtg ccacgtcccc
    17161 gcgtcattca acgaaccgaa cgaatcacaa aagagatgag caacaac
//
LOCUS       DBMDTI010000017        16730 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1095_length_16730_cov_4.133502, whole genome shotgun sequence.
ACCESSION   DBMDTI010000017 DBMDTI010000000
VERSION     DBMDTI010000017.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 16730)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16730)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16730
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1095_length_16730_cov_4.133502"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="ACZPB6_01940"
     CDS             complement(<1..113)
                     /locus_tag="ACZPB6_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR1183272.1"
                     /translation="MKILIVGAGRIGSSVAESLVSEANDITVVDQDAERIGS"
     gene            complement(110..1018)
                     /locus_tag="ACZPB6_01945"
     CDS             complement(110..1018)
                     /locus_tag="ACZPB6_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1183273.1"
                     /translation="MHTVLIVDDEYGIRELLSEILEDEGYQVLTAENAKIAQSLVGKH
                     DFDLILLDIWMPDMDGISLLKEWYSHNTVRCPIVMMSGHGTIETAMEATRFGAMDFLE
                     KPISMQQLLKTCKQMIQFWEHEKVSRPRENEQAKAASVALAGWKKASCSPLNVSLANL
                     NDKSRFCDHFILTDVPLRIGPQARLPIIDVPELHFEFNLNLPLRELRDSLERAYITRV
                     AQFYGGSVTMLAKHAGLERTHLYRKIRQLGLELKKTTRKDESDHIAAGDTMEDLSEET
                     SDTPDNKKCFQIHQKTTDVNNGSDDS"
     gene            complement(1027..3288)
                     /locus_tag="ACZPB6_01950"
     CDS             complement(1027..3288)
                     /locus_tag="ACZPB6_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1183274.1"
                     /translation="MPRWLRYSTLIAISFASIAMLGLAISLFNPKLFDRYFPFLLVLN
                     AVIAAVLFCVVTTLCLRLARRQRSKQYGSHMTAKLALTTALISVVPTLTVYAISTAFI
                     SRSLQDSFSPRVEASLDAGVRITQEMLAKQEQSTQELARDFADALAGTPESLMLHDLL
                     KLLEPHSGTEALVLTSTGSAVAAAGSRINVLMPDLPSQLQLKTAKTTGIYSIVDGDTL
                     FEAPEATGGSSDLRIRVIIPISTLHAPSLSDFKTTGLLAKTASSQQLFLQLTQPVDKT
                     IGLHAATLVAGYREYQQLAYSQSSMATLYALTLSLIVLLAVFASVLAALSFARRTTSP
                     VMQLAEGTRRVATGDFMPIREFQGGDEINVLTRSFNSMIKEVSEARRGLELQRLQAEQ
                     AQAFLERVLGNISSGVVVVDHWYTVVTANQAARRIMGEQNVMTGASLRDTLPEFVSTL
                     EEYQHRESIGTAPEPLDFELEQNGVTIPLYLKTTPMQLGTQTGSVLVFDDVTALMKAQ
                     RATAWGEVARRLAHEIKNPLTPIRLSAERLAWKLHAKLTEEKDLQLLQRTTNTIIEQV
                     DTLRQMVNDFREYARMPAASLVPLNLNEFMETVLQLYHDAGHAISFVPGEGLPEIDAD
                     PAQLRQVFHNLISNSLEAVTEGELVRISIRTEALESQYHAGAIGAVKLSIEDNGPGFT
                     DKILNAAFDPYVTTKPTGTGLGLPIVKKILDDHGAAIKLKNRSNEETGEILGASVEIV
                     FKVSRLEHNPISN"
     gene            complement(3298..3903)
                     /locus_tag="ACZPB6_01955"
     CDS             complement(3298..3903)
                     /locus_tag="ACZPB6_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HIR1183275.1"
                     /translation="MYKATRRQTLKLALLAALGASSMTAPEARAADATFMRAALTLDE
                     PGERSGLFLSSSFEFDLPQALSEALHRGIALYFVHEFRLYKERWYWIDKATIRCRFMI
                     RLAFDPLTRRYRLSYNGLSLSFDTLEQALPYIKNIRRWRVGPSNAVRNAEDYAAELRF
                     YLDTDKLPKPMQVVNTGSSDWTLTSDWIELPIPAEVIAPAE"
     gene            complement(3905..5245)
                     /gene="rsmB"
                     /locus_tag="ACZPB6_01960"
     CDS             complement(3905..5245)
                     /gene="rsmB"
                     /locus_tag="ACZPB6_01960"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR1183276.1"
                     /translation="MSIAASAPHKQAPFSKIVSAVASARTQLRAGASLNSVIRPALLN
                     VPLESRPAAQALIYESVRRCARTTALVGLLCSRQPSAEVLSVLETAFAAFSLGRLAVF
                     TVVSETVTAAKANPQTRAASGFINAVLRRYLREKDELEKKIASRDEVRFNAPAWWIGR
                     IRTIYPKDADRILELGTRHPPMTLRVNVRLMTVEDYLDRLKAAGLEARRVGPEAIELV
                     TPVPVDRIPGFADGLSSVQDAGTQLAAHLLPVKAGDRVLDACSAPGGKTAHILERADC
                     AMTALEIDPARAVKVRETLDRLKLKADIRTADAGDTASWWNGKPFDAILLDAPCTASG
                     VVRRQPDTPWLRRPDDIKKLASEQKRLLKTLWPLLRKGGRMLYATCSIFPEEGTGQMK
                     AFLAATPDARLVPLFAGNDGMLTLLPEESGEWRSDAQLPTVHDGFFYALLEKQA"
     gene            complement(5242..6198)
                     /gene="fmt"
                     /locus_tag="ACZPB6_01965"
     CDS             complement(5242..6198)
                     /gene="fmt"
                     /locus_tag="ACZPB6_01965"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR1183277.1"
                     /translation="MKIIFAGTPDFAAVALKDLIDAGHEIALVLSQPDRPSGRGMKLT
                     PSPVKKLALEHGIPVITPVTLSLKKAPEESARIHADLKAVGADLLVVAAYGLILPQAV
                     LDCAKGIGRNGDIRAINIHGSLLPRWRGAAPVARAIESGDAESGVVLMKMELGLDTGP
                     MIRTVRTPITQEDTSDTLMMRLAELGAELLVESLRTPDELDWTPQPEEGVTYAEKLLK
                     TEAVIDWTLPAKVLANKVRAFNPFPGTVFDKDGVPVKIWQAEALELSGRPGEVLDTTN
                     GLVIACGEGALRATVLQKPGKPKMAFGPFLQSLPFTVGEVVK"
     gene            6308..7207
                     /locus_tag="ACZPB6_01970"
     CDS             6308..7207
                     /locus_tag="ACZPB6_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1183278.1"
                     /translation="MDALYYNGFKLAYRRYGASDAPAVVLIMGLGMSSAAWPTSLIAH
                     LVREGFQVITPDNRDCGESSRCTDMKADGRNVASAVAQALLRRKVTSAYALEDMALDV
                     ERLLNYLSIRRAHIVGISMGGMIAQVAAVQSPNRVASLTSIASASGNPRTGFGRLNAV
                     WTLLRKPDNLNTEEGLRDYFARVFTTLSGPAYKPSGEELESMLGMLGSLHYDPDGTAR
                     QLLAILASGDRSWQLRRLAAPSLIIHGTADPLLPLAAGRETADLIPNARFEVIEGMGH
                     QLPEALVPELGALIAEHCHRHPA"
     gene            complement(7229..7741)
                     /gene="def"
                     /locus_tag="ACZPB6_01975"
     CDS             complement(7229..7741)
                     /gene="def"
                     /locus_tag="ACZPB6_01975"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR1183279.1"
                     /translation="MALLPIIQYPDPVLAAKAEPVTEFNDELRKLAADMAETMYAAPG
                     VGLAANQVGSLKRIVVIDITDEKNNLLVLVNPYIKSHTEELAEFEEGCLSLKGLYEKV
                     SRPDGVTVCAQDLDGKPFEIEATGLLAVCLQHELDHLDGTVFIDHLSRLKKDRAKTKL
                     RKLRKNDKQA"
     gene            7843..8739
                     /locus_tag="ACZPB6_01980"
     CDS             7843..8739
                     /locus_tag="ACZPB6_01980"
                     /inference="COORDINATES: protein motif:HMM:NF014533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR1183280.1"
                     /translation="MTPETGREAECWIKLSLLTVLSVPEILRLLQQTGGVEALFDASS
                     GTLTKLAGETAAREIREVNSSGEAERILSWLSETKGVGFITLADPRYPRLMIEAGAAP
                     VLLWYRGDAGLLARPVLTVGGSTHPDREALYNAEVFGEALGTRNELLLSTGHFPGTET
                     VFLASVLRTGGRALVWQPCGPDRVWPSEGRDLLHELLDKGGLIMTAVAPGFGFSPEGR
                     ELQTCCRMASAQALLALNAGTGSHLFSLARTAADWGRDVMAVPGSIHSPFARGCHRLI
                     RQGARLVESSDDILAELRLAGS"
     gene            8905..11463
                     /locus_tag="ACZPB6_01985"
     CDS             8905..11463
                     /locus_tag="ACZPB6_01985"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HIR1183281.1"
                     /translation="MSLSLVIAEKPSVAGDIARALGGFTRDGDFWVRDDMVIGSAVGH
                     LLEITAPEEFDVKRGRWTFKNLPVLPPYFDLKPIKKSEEKLKALSKKIRSRAVTEVIN
                     ACDAGREGELIFRYIMQSCGSKKPVKRLWLQSMTKNAIQEGFRHLRTDDEMKPLEAAA
                     RCRSEADWLVGINGTRAMTAFNSKEGGFFLTTVGRVQTPTLAIVVKREEEINAFVPKG
                     YWEVSAVFGVSAGEYEGIWIDPNFRKDKDDPDRKAERLWTEDEARRIAAACRNGMGKI
                     EETSKRSRQLSPLLFDLTSLQREANSRFGYSAKTTLSIAQALYEKHKVLTYPRTDARA
                     LPEDYMPTVRDTLNALGGLTDYAAFSSKILTQNWVRPDKRIFDNTKISDHFAIIPTGQ
                     LPKTLNEVEQKIFDLVVRRFLAVFYPAAEYDVTVRITTVGAHQFKTEGKVLAEPGWLE
                     VAGKGRTQREALTPVKPGEPAAVKDVVVSAMQTKAPARYTEATLLSAMETAGKKLEDD
                     ELRGAMADKGLGTPATRASIIEGLIEQKYMRREERDLHPMAKAFQLITLLKGLKIAEL
                     SEPRLTAEWEQKLRLIEEGKFQSDEFMREIRRLTENVVNMAKQYEGNSVPLENPRRIE
                     APCPQCGGEIVENYRRFACTTPGCEFSIAKHPSGRMLEQAEVEELLNTGRVGPLSGFI
                     SKRGFPFEAELILKKDETDGLWKMQFDFGEEEKAEVTDEEIESAPVVGVCPCCGARVL
                     EMPAAYQCEKNIRGEKKCTFRISKTILSRDITSEEVTELLANKRTQLLSGFISKKSKR
                     AFKAFLIVKGNGSIAFEFQPSKKDAEATESGEENSEAKPRKTTRRTTKKKTAAE"
     gene            complement(11543..12874)
                     /gene="paeA"
                     /locus_tag="ACZPB6_01990"
                     /gene_synonym="ytfL"
     CDS             complement(11543..12874)
                     /gene="paeA"
                     /locus_tag="ACZPB6_01990"
                     /gene_synonym="ytfL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine export protein PaeA"
                     /protein_id="HIR1183282.1"
                     /translation="MSVYTELFSLFILVCLSAFLSCAEISLASARRSRLNVLADDGDR
                     RARLVLDLQEHPGQFFSAIQIGTNALALLGGVVGEAAFSPFFKWCLSFVFTPEQIHGV
                     DFVISFVLATLLFVIFADLLPKRLAVAKPEETAMTVVRPTFVMIKLLRPLVFVLEKFS
                     DALMRSLGFSTKRQDTITSEDILASVGAATAAGVLAPQEEAVIQNVFDLESRLVPSAM
                     TPRENIVFFTLDDPEEEVRVKISSVPYNRFLICDKDLDHIIGYVDSMHILRQVLNNKP
                     ISFKEPGVVQTCPFIPDSLTLSEVLDVFQRSRADFAVILNEYALVVGVITINDVMSTV
                     MGELVVNADELQIVEREDGTWLVDGSTPIDDLEHALDIDEFPEDTTYETVAGFMMYML
                     RKIPKRTDKVVWAGYTFEVIDVDNNKIDQILVTRNKRPAPVADAEEPAKAD"
     gene            13169..14191
                     /locus_tag="ACZPB6_01995"
     CDS             13169..14191
                     /locus_tag="ACZPB6_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1183283.1"
                     /translation="MKMKLLFVAALLAAGAVAMPAVAGVTLDNVKKRGHVLCGVNTSA
                     PGFSSADSQGKWRGLDVNICRSVAAAVLGDADKVKYVPLSSPQRFTALQSGEIDVLAR
                     NTTWTMTRDASQGSVFVAVSYYDGQGFIVPKEFGIKTAKELDGATVCVQSGTSSEKTL
                     ADYFNSHKMSYKTVVFDTTEATQSAFLSGRCQVYTTDMSDLAGMRTLAQDPSKYEILS
                     EVISKEPLGPAVRRGDDEWFQIVRWSFNTMVEAEELGITKANADELRKTSANPNLQRL
                     LGQSDDMGKLIGLDKEWSYRIIKQVGNYGESWTEFFGPKTPLNLPRGLNRLWTDGGLM
                     YAMPIR"
     gene            14284..15459
                     /locus_tag="ACZPB6_02000"
     CDS             14284..15459
                     /locus_tag="ACZPB6_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1183284.1"
                     /translation="MLSRESFGIYGDAAWKEREKARKRREYTIQAVLILILVGFLAAV
                     SMNVADNLAERHINSGFAFLGNPAGFDIGEALIPFTNTDHMSRAFLVGMLNTIKVSAV
                     SIIAATVLGVVVGLMRLSRHPMLRFLGTAHVEFYRNIPLLVLLLAVYLAVTELLPAGR
                     TALHVGDWVFLSKSGLQYASPLMGGWAMWIAAGAGAACGFASAFFARRRMTGLMANTT
                     GIAVFAGVFIVAWVLCGVVSGWDHPVQTRFALRGGSQISPEFLALSLGLTLFTSASIA
                     EIVRAGVLAVKAGQWDAGLALGMTMTETVSYVIFPQSMRLVIPPLASQYMNLTKNSSL
                     AVMVGYPDLVNIGNTVINVSAQALEVICIIMAVYLTLNLVISVVMNALNARIMRAPQ"
     gene            15470..16564
                     /locus_tag="ACZPB6_02005"
     CDS             15470..16564
                     /locus_tag="ACZPB6_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1183285.1"
                     /translation="MITESSFSGSLSQRIRRRYFYSPMASLFTVVVSAVIAFIVGKLV
                     LWGLVNAVWTPDVQACGQSSGACWGFVAEKWRLIIFGRYPYHLQWRAAAATGIVLAML
                     VVSSFPFMWRKPYLRTLGMLWSAAVVFFFILMLGGVFGLESISTDMWGGLPLTIVLTL
                     VGMGASVPLGILLALGRRSKMPAVASVSTGYIELVRGVPLITVLFVAAFIFPLLLPAG
                     VRIDPFWRISVAIALFQAAYMAEIVRGGLQTIPRGQFDAAASLGLSQRQVYTAVILPQ
                     ALVAIIPAFVNSLLSCFMDTSLVTVVSMYDLTGSLRLALGDALWRGYFIEGYVFIALV
                     YFFFSFIVSRYSQWLEVYLTGEKDRGHAAE"
     gene            16607..>16730
                     /locus_tag="ACZPB6_02010"
     CDS             16607..>16730
                     /locus_tag="ACZPB6_02010"
                     /inference="COORDINATES: protein motif:HMM:NF020551.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtaL-like protein"
                     /protein_id="HIR1183286.1"
                     /translation="MRKVHEHIIQIPPFVTRDQLWNGLEKAARHPDRFVEHMEGC"
BASE COUNT         3083 a         4947 c         5443 g         3257 t
ORIGIN      
        1 gatccgatgc gctcggcgtc ctggtcaacg acggtgatgt cgttggcttc ggagaccaga
       61 ctctcggcga ccgagctgcc gatgcggcct gcgcccacaa taaggatttt catgagtcgt
      121 ccgagccgtt attcacgtcg gtggttttct gatggatctg gaagcacttc ttgttgtcgg
      181 gagtgtccga ggtttcctcg gaaagatctt ccatcgtgtc gccagccgct atgtgatcgg
      241 attcatcctt tctggtggtt ttcttgagct ccaggccgag ctggcgaatc ttgcggtaga
      301 ggtgagtgcg ctccagaccc gcatgcttgg caagcatcgt gaccgagccg ccgtagaact
      361 gggcgacgcg ggtgatgtag gcgcgttcaa gactgtcgcg aagctcacgc agcggcaggt
      421 tgaggttgaa ctcgaaatgg agttcgggaa cgtcgatgat cggcaggcgg gcctgaggac
      481 cgatgcgaag cggcacgtcc gtgagaatga agtggtcgca gaacctggac ttgtcgttga
      541 ggttggcgag cgaaacattc agaggggagc aggaagcttt tttccagccc gccagagcga
      601 cgctggcggc cttggcctgt tcgttttcgc ggggacgcga aaccttttcg tgctcccaga
      661 actggatcat ctgcttgcac gtcttcagaa gctgctgcat cgagatcggc ttctcgagga
      721 agtccattgc accgaagcgc gtggcctcca ttgcggtttc aatggtgccg tggcctgaca
      781 tcatcacgat ggggcacctg acggtgttat gcgaatacca ctccttgagc agggaaatgc
      841 cgtccatgtc gggcatccag atgtcaagga gaatcaggtc gaagtcgtgc ttgccaacta
      901 ggctctgagc gattttggca ttttctgcgg tcaggacttg gtagccctcg tcctccagaa
      961 tctctgaaag gagctcgcgg atgccgtatt catcgtcaac gatcaagact gtatgcatgt
     1021 tgtggctcag ttggaaattg ggttgtgctc tagtcgagag actttgaaga caatttcgac
     1081 agatgcgccg agaatctcgc cggtttcttc gttgctgcgg ttcttgagct tgatggcggc
     1141 gccgtggtcg tcaaggatct tcttcacgat cggcaggccg agccccgttc ccgtcggctt
     1201 ggttgtcaca taggggtcaa aggcagcatt caggatttta tccgtaaagc cgggcccatt
     1261 atcctcaata gagagtttaa cagcgccgat tgcgcctgca tggtattggg attccagcgc
     1321 ttccgttctg atcgaaatgc gaacgagctc accttccgtc acggcctcga ggctgttgga
     1381 aatcaaattg tggaacacct gcctgagctg ggcggggtcc gcgtcgatct ccggcagccc
     1441 ctctccgggg acgaagctga tggcgtgacc tgcatcgtgg taaagctgca ggacggtttc
     1501 catgaactcg ttgaggttca gcgggacaag ggatgcggcg ggcatgcgcg cgtactccct
     1561 gaagtcgttg accatctgtc tgagcgtgtc gacctgctcg atgatggtgt tggtcgtcct
     1621 ctggagaagc tgcaggtcct tttcctcggt cagcttggcg tgaagcttcc aggccagcct
     1681 ttcggcggaa agcctgatgg gcgtgagcgg attcttgatc tcgtgcgcaa ggcggcgggc
     1741 gacctcgccc caggcggtgg ctcgctgcgc tttcatgagg gcggtcacgt cgtcgaagac
     1801 gagcacggac cccgtctggg tgccgagttg catgggcgta gttttcaggt agagcggaat
     1861 cgtcacgccg ttctgttcaa gctcgaagtc gaggggttcg ggggccgtgc cgatgctctc
     1921 cctgtgctga tattcctcga gggtcgaaac gaattcgggc agcgtatcgc gaagggaggc
     1981 gcccgtcatc acgttctgct cgcccatgat gcggcgtgcc gcctgattgg ccgtgacgac
     2041 cgtgtaccag tggtccacga ccacgacgcc cgacgaaatg ttgccgagca cgcgctccag
     2101 aaacgcctgg gcctgctcgg cctgcaggcg ctgcagctcg aggccgcgtc tggcttcgga
     2161 cacttccttg atcatcgagt tgaaggagcg ggtcagcacg ttgatttcgt cgccgccctg
     2221 gaactcgcgg atcggcatga agtcgcccgt ggccacgcgt ctggtgcctt cggcaagttg
     2281 catgacgggc gacgtcgtcc tgcgtgcaaa ggacagcgca gcgagaacgc tcgcgaagac
     2341 ggcgagcagg acgatgagcg agagcgtcag cgcgtaaagc gtggccatgg agctctggct
     2401 gtaggccagc tgctggtact cgcggtagcc tgcgacgagc gtggccgcat gaagcccgat
     2461 tgtcttgtcg acgggctggg tcagctgcag gaagagctgc tggctgctgg cggtcttggc
     2521 aagcagtccc gtggtcttga agtcggagag ggacggagcg tgcagcgtcg aaatggggat
     2581 gatgacgcgt atgcgtaggt cgcttgagcc gcctgtcgct tcaggagcct cgaaaagcgt
     2641 gtcgccgtcg acgatgctgt agatgcccgt cgtcttggcg gtcttgagct ggagctgcga
     2701 gggcaggtcg ggcatcagca cgttgatgcg ggagcctgca gccgccacgg cggagcccgt
     2761 gcttgtgagg acaagagcct cggttcccga atgcggctcc agaagcttga gcaggtcgtg
     2821 tagcatcagg gattcgggcg tcccggcgag tgcatccgcg aagtcacgtg ccagctcctg
     2881 cgtggactgt tcctgcttgg cgagcatctc ctgcgtgatg cgaacgcctg cgtcgagcga
     2941 ggcctccacg cgtggagaga aggagtcctg aagcgagcgg ctgatgaagg ccgtggagat
     3001 cgcatagaca gtcagcgtcg gcaccacgct gatgagggcc gtcgtgagcg caagcttcgc
     3061 cgtcatgtgc gagccgtact gctttgagcg ctggcgtctt gccagacgca ggcacagcgt
     3121 cgtcacgacg caaaaaagga cggcggcgat gacggcgttc aggacgagca ggaacgggaa
     3181 gtacctgtcg aacagcttgg gattgaagag actgatcgcc agcccgagca tggcgatgct
     3241 cgcaaaggag atggcgatca gggtgctgta gcgtaaccag cggggcatgg ctgtctctta
     3301 ctccgcggga gcgatgactt ccgcaggaat cggaagttct atccagtcgc tggtgagcgt
     3361 ccagtcgcta ctgcccgtgt tgacgacctg catcggcttg ggaagcttgt ccgtgtccag
     3421 atagaagcgc agttcggccg cgtagtcctc cgcattgcgc acggcgttcg agggaccgac
     3481 gcgccagcgc ctgatgttct tgatgtaggg cagggcctgt tcaagcgtgt cgaaggagag
     3541 cgaaagcccg ttgtaggaga ggcggtaacg gcgcgtgagc ggatcgaacg ccagacggat
     3601 catgaagcgg cagcggatcg tggccttgtc gatccagtac caccgctcct tgtagagcct
     3661 gaattcgtga acgaaataga gcgcaatgcc cctgtgaagc gcctcggaga gcgcctgagg
     3721 caggtcaaac tcaaaggagg agctcaggaa gaggcccgac cgctcgccag gttcgtcaag
     3781 cgtgagcgcg gcgcgcatga aggtcgcgtc ggccgcccgc gcctcgggcg cggtcatgga
     3841 cgaagcgccg agcgccgcga gcagcgcaag tttcagggtc tggcgtcggg tcgctttgta
     3901 catgtcaggc ttgcttttcc aaaagggcat agaagaagcc gtcatgcacg gtgggcagct
     3961 gggcgtcgga gcgccattct cccgattcct cgggcagaag agtcagcatg ccgtcattgc
     4021 ctgcaaacag ggggacgaga cgggcgtcgg gcgttgcggc caggaatgcc ttcatctgtc
     4081 ccgtgccttc ttcggggaag atcgagcacg tggcgtagag catcctgccg cccttgcgga
     4141 gcaggggcca gagcgtcttg aggagcctct tctgttctga ggcgagcttc ttgatgtcgt
     4201 cggggcgacg gagccacggc gtgtcgggct ggcggcgcac cacgccgctt gccgtgcacg
     4261 gcgcgtcaag cagaatggcg tcgaagggct tgccgttcca ccagcttgcc gtgtcgccgg
     4321 cgtcagccgt tcgaatgtcg gccttgagtt tgagtcggtc gagagtctcg cgaaccttga
     4381 cggctcgggc gggatcgatc tccagcgcag tcatggcgca gtccgcgcgc tcgagaatgt
     4441 gggcggtctt gccgccggga gccgagcagg cgtccagcac ccggtcgccc gccttcacgg
     4501 gcaacagatg cgcagcaagc tgcgttcctg catcctgaac ggaactcagc ccgtcggcaa
     4561 agccgggaat gcggtcgacg gggacgggcg tgacgagttc gatggcctcg gggccgactc
     4621 ggcgggcttc aagccctgcg gccttcagcc ggtcaagata gtcttcgacg gtcatgaggc
     4681 ggacgttgac gcgcagcgtc atcggaggat gacgcgtgcc cagctccaga atgcggtccg
     4741 catcctttgg ataaatcgtg cggatgcggc caatccacca agcgggagca ttgaagcgga
     4801 cctcgtcgcg ggaggcgatc ttcttttcca gttcatcctt ctcgcgcaga tagcggcgga
     4861 gcacggcgtt gatgaagcct gaggcggcgc gggtctgcgg gttcgccttg gccgccgtga
     4921 ccgtttcgga cacgacggtg aagactgcga gcctgccgag cgagaaggcg gcgaaggcgg
     4981 tctcgagcac ggagagcacc tcggccgagg gctggcggct gcaaagcagt ccgacgagtg
     5041 cggtcgttct cgcgcagcgg cgcacgctct catagatgag cgcctgtgcg gcgggacggc
     5101 tttccagcgg gacgttgaga agcgcaggtc ggatgacgga gttgagcgag gcgcctgcgc
     5161 gcagctgcgt gcgtgcggag gcgacggcgc tcacgatttt ggaaaaaggc gcctgcttgt
     5221 gcggcgcgga cgcggcgatg ctcacttgac gacctcacca acggtaaagg gcaggctctg
     5281 aaggaaggga ccgaaggcca tcttgggctt gccgggcttc tgcagaaccg tggcgcgaag
     5341 agcgccttcg ccgcaggcga tcacaagacc attcgtcgtg tcgagcactt cgccgggacg
     5401 accagagagc tcgagcgctt cggcctgcca gatcttgacg gggacgccgt ccttgtcgaa
     5461 tacggttccc gggaagggat tgaatgcgcg aactttgttg gcgaggactt ttgcgggaag
     5521 ggtccagtcg atgacggctt ccgtcttcag aagcttctcg gcataggtga cgccttcctc
     5581 gggctggggc gtccagtcga gttcgtccgg cgtcctgagg gattcgacga ggagctctgc
     5641 gccgagttcg gccaggcgca tcatgagcgt gtcggaggtg tcctcctgcg tgatgggcgt
     5701 gcgcacggtg cggatcatcg gacccgtgtc aaggcccagc tccatcttca tgagcacgac
     5761 gccgctttcc gcatcgccgg actcgatggc gcgcgcaacg ggcgcggcgc cgcgccagcg
     5821 gggcagaagg ctgccgtgga tgttgatggc gcggatgtcg ccgttgcggc cgatgccctt
     5881 ggcgcagtcg agaacagcct gcggaagaat gaggccgtat gcggccacga cgagcaggtc
     5941 ggcgccgacg gccttgaggt ccgcgtgaat gcgggcggac tcttcgggtg ccttcttcag
     6001 gctcagcgtg accggcgtga tgacgggaat gccgtgctca agggcgagct tcttgacggg
     6061 cgagggagtg agcttcatgc cgcggccgct cgggcggtcg ggctgcgaga gaacgagggc
     6121 gatctcgtgt cccgcgtcaa tgaggtcctt gagcgcgacg gccgcgaagt cgggcgtgcc
     6181 tgcaaaaatg atcttcatac gtttggactc cggcgacgct ggatggggag cgttttcaag
     6241 tgtcaaaata caatgatggt actagtgtac ggtactttag gcagagaggc gcaaagagag
     6301 aaacgacgtg gacgcacttt attacaacgg attcaagctg gcctaccggc gctatggcgc
     6361 ctcggatgcg cccgccgtcg tgctgatcat gggccttggc atgtcgagcg ccgcttggcc
     6421 gacaagcctc atcgcacacc ttgtgcggga aggctttcag gtcatcacgc ccgacaaccg
     6481 cgactgcggg gagagctccc gctgcacgga catgaaggcg gacggaagaa acgtggcctc
     6541 agccgtggcg caggcgcttc tgcgccgaaa ggtgacgagc gcctacgcgc ttgaggacat
     6601 ggcgctcgac gtggagcgcc tcctcaatta cctgtcgata cgcagggcgc acatcgtcgg
     6661 catctccatg ggcggcatga tcgcacaggt ggcggccgtg caatccccca atcgcgtggc
     6721 atcgctcacg tcgatcgcct ccgcttcggg caacccgaga acgggattcg gtcgtctcaa
     6781 tgcggtctgg actcttctga gaaagcccga caatctgaat acggaggaag gactgaggga
     6841 ttacttcgcc cgcgtgttca ccaccctttc agggccggcg tacaagccgt cgggcgagga
     6901 actcgagagc atgctgggca tgctggggag cctgcactac gatcctgacg gaacggcgcg
     6961 gcagctgctg gccattctgg cgagtgggga caggtcctgg cagctcaggc gtctggcggc
     7021 gccctcgctg atcattcacg gcacggcgga tccgcttctg ccgcttgcgg caggcaggga
     7081 gaccgcagat ctgattccca acgcacgctt cgaggtgatc gagggcatgg ggcatcagct
     7141 ccccgaggct ctggtgcccg aacttggcgc gctcatcgcg gagcactgcc acaggcatcc
     7201 tgcctgacgc ggcctgcgcc gctcaggctc aggcctgctt gtcgttcttg cgaagcttgc
     7261 gcagcttcgt cttggcgcgg tccttcttga ggcggctgag atggtcgatg aagaccgtgc
     7321 cgtcaaggtg gtcaagctca tgctgcaggc aaacggcgag aaggccggtg gcctcgattt
     7381 cgaaaggttt gccgtcaagg tcctgggcgc acacggtgac gccatcgggg cggctcacct
     7441 tttcataaag tcccttgagg gaaaggcagc cttcttcgaa ttcggcaagc tcctccgtgt
     7501 gggacttgat gtagggattg acgaggacga gaaggttgtt cttctcgtcg gtgatgtcga
     7561 tgaccacgat gcgcttgagg ctgcccacct gattggccgc gagcccgacg ccgggggccg
     7621 catacatggt ttcggccatg tcggcggcaa gcttgcggag ctcgtcgttg aactcggtga
     7681 cgggctcggc cttggcagcc agaacgggat cgggatattg gataatgggc agaagagcca
     7741 cgagacgctc ctagaaagag ttgaacttgt aaaaccgtct attttagatc agaactgccg
     7801 tccggactca agagggcggg cggattcacc acgggaaggg acatgacgcc tgaaacgggc
     7861 cgcgaggccg aatgttggat caagctttct ctgcttactg tgctttcggt gcctgagatc
     7921 ctcaggctcc tgcagcagac gggcggcgtc gaggcgcttt tcgacgcctc gtcgggaacc
     7981 ctgacgaagc ttgccggcga gacggcggcg cgcgagatcc gcgaggttaa ttcgagcgga
     8041 gaggcggaac gcatcctgtc gtggctgtcc gaaacgaagg gagtcggctt catcacgctg
     8101 gccgatcccc gctatccccg cctcatgatc gaggcgggcg ccgcgcccgt gcttctctgg
     8161 taccgcggtg acgcgggcct tctcgccagg cccgtgctta cggtgggcgg cagcacgcac
     8221 cccgacaggg aggctctgta caatgcggag gttttcggcg aggcgctcgg aacccgcaac
     8281 gagcttctgc tctcgacggg gcactttccc ggaacggaga cggtgtttct cgcaagcgtg
     8341 ctccgcacag gcggcagggc gctcgtctgg cagccctgcg gacccgacag ggtctggccg
     8401 tccgagggac gagatcttct ccacgagctt ctggacaagg gaggcctcat catgaccgcc
     8461 gttgcgccgg ggttcggatt ctcgcccgag gggcgggagc tgcagacgtg ctgccgcatg
     8521 gcctccgcgc aggcgcttct ggcgctcaat gcaggcacgg gctcccatct cttcagcctc
     8581 gcgagaacgg cggccgactg gggcagggac gtcatggccg tgccggggtc catccactct
     8641 cccttcgcca ggggatgtca caggctgatc cgtcagggag cgaggcttgt cgagtcttcg
     8701 gacgacattc ttgccgagct gcgtctggca gggagttgag tccggctgcc tgtagttggt
     8761 acgctatgaa tatttgcacg agggcattcc ctgcggcgtc tgttgcataa tgatgtccaa
     8821 cgcaggcgca tgccgccatt catttgtttt cagtttgctg tgcccgccgg ttccacgata
     8881 actgcggtca tggagatgca tcgtatgtcc ttatctcttg tcattgctga gaagccttcc
     8941 gttgcaggcg atattgcgcg agctctcggg ggcttcacgc gcgacggcga cttctgggtc
     9001 cgtgacgaca tggtgatcgg ctccgccgtc ggtcatctgc tcgaaattac tgcgcccgaa
     9061 gagtttgatg tcaagcgcgg ccgctggacc ttcaagaacc ttcccgtgtt gccgccctac
     9121 ttcgatctca agccgatcaa gaagtccgaa gagaagctca aggctttgtc gaagaagatc
     9181 cgaagccgtg ccgtgaccga agtcatcaac gcatgcgacg cgggacgcga gggtgaactg
     9241 atcttccgct acatcatgca gtcctgcggc agcaagaagc ccgtcaagcg tctctggctc
     9301 cagtccatga cgaagaacgc cattcaggag ggcttccgtc atctgcgcac cgacgatgag
     9361 atgaagcccc ttgaggccgc cgcccgctgc cgatcggaag ccgactggct tgtcggcatc
     9421 aacggcacgc gcgccatgac cgcctttaac tccaaggaag gcggcttctt cctgacgacc
     9481 gtcggccgtg ttcagacgcc gacgctcgcg atcgtcgtca agcgtgagga ggagatcaat
     9541 gcattcgtgc ccaagggcta ctgggaggtt tccgccgtgt tcggcgtctc cgcgggcgaa
     9601 tacgagggca tctggatcga cccgaacttc cgcaaggaca aggatgatcc cgaccgcaag
     9661 gccgagcgcc tctggacgga ggacgaggcc cgcaggattg ccgcggcctg ccgcaacggc
     9721 atgggcaaga tcgaggagac ctcgaagcgc tcccgtcagc tttcgcccct gctcttcgac
     9781 ctgacgagcc tgcagcgcga ggccaacagc cgcttcggct attccgccaa gacgacgctt
     9841 tccatcgcgc aggcgctcta tgaaaagcac aaggttctca cctatccgcg aaccgacgcc
     9901 cgcgcgcttc ccgaagacta catgccgacc gtgcgcgaca cgcttaacgc actgggcggc
     9961 ctcacggact acgcagcctt ctcgtccaag atcctgacgc agaactgggt tcgtccggac
    10021 aagcgcatct tcgacaacac gaagatcagc gatcacttcg ccatcattcc gacggggcag
    10081 cttccgaaga ctctcaatga ggtcgagcag aagatcttcg atctggtcgt gcgccgcttt
    10141 ctggccgtct tctatcctgc ggccgagtat gacgtgacgg tccgcatcac gaccgtcggc
    10201 gcccaccagt tcaagaccga gggcaaggtg cttgccgagc ccggctggct cgaagtggcc
    10261 ggcaagggcc gtacccagcg cgaggcgctt actcccgtca agcccggcga accggccgcc
    10321 gtgaaggacg tcgtcgtttc cgccatgcag accaaggctc ccgcccgcta cacggaagcc
    10381 acgctcctga gcgctatgga gaccgcaggc aagaagcttg aggacgacga gctgcgcggc
    10441 gccatggccg acaagggcct gggcacacct gccacccgcg cctcgatcat cgagggcctg
    10501 attgagcaga agtacatgcg ccgtgaggag cgcgatctgc atccgatggc taaggccttc
    10561 cagctcatca cgctgctcaa ggggctcaag atcgccgaac tgagcgagcc gcgcctcacc
    10621 gccgagtggg agcaaaagct gcgcctcatc gaggagggca agttccagag cgacgagttc
    10681 atgcgtgaga tccgacgcct gacggagaat gtcgtcaaca tggccaagca gtacgagggc
    10741 aacagcgtgc cgcttgaaaa tccgcgccgc attgaggcgc cctgtccgca gtgcggcggc
    10801 gagatcgtcg aaaactaccg ccgcttcgcc tgcacgacgc ccggctgcga gttctcgatt
    10861 gccaagcatc cttcgggccg catgctcgaa caggctgagg ttgaagagct cctcaacacg
    10921 gggcgcgtgg gaccgctttc cggcttcatc agcaagcgcg gcttcccgtt tgaggccgaa
    10981 ctgattctca aaaaggacga gaccgacggc ctctggaaga tgcagttcga cttcggcgag
    11041 gaggaaaagg ccgaggtgac ggacgaggag atcgaaagcg ctcccgtcgt cggcgtctgc
    11101 ccgtgctgcg gcgcccgcgt gcttgaaatg cccgcagcct accagtgcga gaagaacatt
    11161 cgcggcgaaa agaagtgcac gttccgcatc agcaagacca tcctttcccg cgacatcacg
    11221 tccgaggagg tcacggaact ccttgccaac aagcgcacgc agttgctctc gggcttcatc
    11281 tcgaagaaga gcaagcgcgc cttcaaggcc ttcctcatcg tgaagggcaa cggcagcatc
    11341 gcgtttgagt tccagccgag caagaaggac gccgaggcga ccgaatccgg cgaggagaac
    11401 tccgaggcca agccccgcaa gacgacccgt aggacgacca aaaagaagac ggcggccgaa
    11461 taagtccgga cccgtctgaa gaaaaagaaa gaggagcccc gatcagagct cctctttctt
    11521 ttcaggcgga acgaatggcg cctcagtcgg ccttggcagg ctcttccgcg tcggcgacgg
    11581 gcgcggggcg cttgttgcgt gtcacgagga tctggtcgat cttgttgttg tcgacgtcga
    11641 tcacctcgaa ggtgtagcca gcccagacga ccttgtcggt cctcttgggg atcttgcgca
    11701 gcatgtacat catgaagccc gcgaccgtct cgtaggtcgt gtcctcgggg aactcgtcga
    11761 tgtcgagcgc atgctcgagg tcgtcgatcg gcgtggagcc gtcgacaagc cacgtgccgt
    11821 cctcacgctc gacgatctga agttcgtcag cgttgacgac gagctcgccc atcacggtgc
    11881 tcatcacgtc gttgatcgtg atgacgccga cgaccagggc gtactcgttg agaatgacgg
    11941 cgaagtcggc gcgggagcgc tggaagacgt cgagcacttc ggaaagcgtc aggctgtccg
    12001 gaatgaaggg gcaggtctgg acgacgccgg gctccttgaa ggagatcggc ttgttgttga
    12061 ggacctgtcg caggatgtgc atgctgtcga cgtagccgat gatgtggtcg aggtccttgt
    12121 cgcagatgag gaagcggttg tacgggacgg aggagatctt gacgcgtacc tcctcctcgg
    12181 ggtcgtcgag cgtgaagaag acgatgttct cgcgcggcgt catggccgag ggcacgaggc
    12241 ggctttcaag gtcgaacaca ttctggatga cggcttcctc ctgcggagca agcacgccgg
    12301 cggccgtggc ggcgccgacg ctcgcgagaa tgtcctcgga cgtgatcgtg tcctgacgct
    12361 tcgtcgagaa gccgagcgag cgcatcagcg cgtcggagaa cttttcaagc acgaagacga
    12421 gcgggcgcag gagcttgatc atgacgaagg tcggacggac gacggtcatg gccgtttcct
    12481 cgggcttggc gacggcaagg cgcttcggga gcaggtccgc gaagatcacg aagagaagcg
    12541 tggcgagtac gaaggagatg acgaagtcga cgccgtgaat ctgttcgggg gtgaacacga
    12601 acgagaggca ccacttgaag aacggcgaga aggcggcttc gccgacgacg ccgccgagaa
    12661 gggcgagggc gttcgtgccg atctggatgg cggagaagaa ctggccggga tgttcctgca
    12721 ggtcgaggac gagtcgggcg cggcggtcgc cgtcgtccgc aagtacgttg agtctggagc
    12781 ggcgggcgga ggcgagggag atttcagcgc aactcagaaa ggcgctgaga cagacgagaa
    12841 tgaaaaggga aaacagctct gtgtaaacgg acataagtcg gagcgggagt ttgaagagtc
    12901 tgccattgta acacgaggat tcgccaaacc ctgtgcttta caggcttttt gtgagcgaac
    12961 gcgtgatttg gcgacatttt gaggtgcgtt tctaggcaga acgaaatagg tgggttgttc
    13021 atgattcgat gtgggacaaa ttttgcttgg cgtttgcagg agtgtctgta aacttaaggt
    13081 cattgacgcc cgaagccctg ccgcgacttt ccctcgaagt gccgggacac aggcagaaca
    13141 actcgatccc gaaaggaagg aactcgaaat gaagatgaag cttttgttcg ttgcagcact
    13201 gcttgccgca ggtgccgttg ccatgcctgc cgtcgctggc gtcacgcttg acaacgtcaa
    13261 gaagcgcggc cacgtgctct gcggcgtcaa cacgtccgct ccgggctttt ccagcgctga
    13321 cagccagggc aagtggcgcg gcctcgacgt caacatctgc cgctccgtgg ccgccgccgt
    13381 tctcggcgac gccgacaagg tcaagtacgt tccgctcagc tctccgcaac gcttcaccgc
    13441 cctgcagtcc ggcgaaatcg acgtgctcgc ccgcaacacc acttggacga tgacgcgcga
    13501 cgccagccag ggttccgtat tcgtggccgt gtcctactat gacggccagg gcttcatcgt
    13561 tccgaaggaa ttcggcatca agaccgccaa ggagcttgac ggcgccacgg tctgcgtcca
    13621 gtccggcacg tcctccgaaa agacgctggc cgactacttc aactcccaca agatgagcta
    13681 caagacggtg gtgtttgaca cgaccgaagc cactcagtcc gccttcctgt ccggccgctg
    13741 ccaggtctac acgaccgaca tgtccgacct cgcaggcatg cgtacgctcg ctcaggatcc
    13801 gtccaagtac gagattcttt ccgaagtgat ctccaaggag cctctcggcc cggccgtccg
    13861 ccgcggtgac gacgaatggt tccagatcgt ccgctggtcc ttcaacacga tggtcgaggc
    13921 cgaagaactc ggcatcacga aggccaatgc cgacgaactg cgcaagacga gcgccaatcc
    13981 gaacctccag cgcctgcttg gtcagagcga cgacatgggt aagctgatcg gtctcgacaa
    14041 ggaatggtcg taccgtatca ttaagcaggt cggcaactac ggcgagtcct ggaccgagtt
    14101 cttcggtccg aagacaccgc tcaacctgcc ccgcggtctg aaccgcttgt ggacggacgg
    14161 cggcctgatg tacgctatgc cgatccgctg atccgcatcc tcggctgccg accggcagcc
    14221 cgttgtttag tacagaaggc tccagaggac gctctggggc cttttgtcag tcaaaaaaaa
    14281 atcatgctat ccagggaatc ttttggtatt tacggcgacg ctgcttggaa ggagcgcgag
    14341 aaggccagaa agcgccgcga atatacgatt caggccgtgc tgatcctcat cctcgtgggc
    14401 ttccttgccg ccgtctcgat gaacgtggcg gacaatcttg cagaacgcca catcaacagc
    14461 ggctttgcct tcctcggcaa tcccgccgga ttcgacatcg gtgaggcgct gatcccattc
    14521 accaatacgg atcacatgtc cagagccttc ctggtgggca tgctcaacac gatcaaggtc
    14581 tccgcagtct ccatcatcgc ggcgaccgtg ctcggcgtcg tcgtgggcct catgcgcctc
    14641 tcgcgccatc cgatgctccg ctttctgggc acggcgcacg tcgagttcta ccgcaacatt
    14701 ccgcttctgg tcctgctcct tgccgtctac ctagccgtga cggaactcct gcctgccggc
    14761 cgcacggccc tgcacgtggg ggactgggtc ttcctctcga agagcggcct tcagtacgca
    14821 tcccccctga tgggcggatg ggccatgtgg atcgccgcag gtgcgggcgc cgcatgcggc
    14881 ttcgcatcgg ccttcttcgc ccgcagacgc atgacgggac tgatggccaa cacgaccggc
    14941 attgccgtgt tcgcaggcgt cttcatcgtc gcctgggtgc tctgcggcgt cgtctcggga
    15001 tgggatcatc ccgtgcagac gcgcttcgcg ctcaggggcg gcagccagat ctctcctgaa
    15061 ttcctcgcgc tctcgctcgg cctcacgctc ttcacgtcgg cctccattgc ggagatcgtc
    15121 cgcgcaggcg ttctggccgt caaggcaggc cagtgggacg cagggcttgc gctcggcatg
    15181 acgatgactg agaccgtgag ctacgtgatc ttcccgcagt ccatgcgact ggtgattccg
    15241 ccgcttgcaa gccagtacat gaacctcacc aagaattcgt cccttgcggt gatggtcggc
    15301 tatcccgacc tcgtcaacat cggcaacacc gtgatcaacg tgagcgctca ggcgcttgag
    15361 gtcatctgca tcatcatggc cgtctatctg acgctcaatc tcgtcatctc ggttgtgatg
    15421 aatgctctca acgcccgcat catgcgggcc ccgcagtaag gaggccggca tgatcaccga
    15481 aagttcgttc agcggctccc tttcccagcg catccgccgc cgttacttct attcgcccat
    15541 ggcgtccctg ttcaccgtcg tcgtatcggc cgtcattgcg ttcatagtcg gcaagctcgt
    15601 gctctggggg ctcgtcaacg ccgtctggac gccggacgtg caggcctgcg gccagagcag
    15661 cggcgcctgc tggggcttcg tcgccgagaa gtggcgcctc atcatcttcg gacgctatcc
    15721 gtaccacctt cagtggcgtg ccgccgctgc aacggggatt gtgcttgcca tgcttgtggt
    15781 gagctccttt ccgttcatgt ggcgcaagcc ctatctgcgc acgctcggca tgctctggag
    15841 cgccgccgtc gtcttcttct tcattctgat gctcggcggc gtgttcgggc ttgagagcat
    15901 ctccaccgac atgtggggcg gcctgccgct tacgattgtg cttacattgg tgggcatggg
    15961 agcatccgtg ccgctcggca ttctgctcgc gctcgggcga cgcagcaaga tgccggccgt
    16021 tgcctccgtg tccacgggct acatcgagct cgtgcgcggg gtgccgctca ttacggtgct
    16081 tttcgttgcc gccttcatct tcccgcttct cctgcctgca ggcgttcgca ttgatccgtt
    16141 ctggcgcatc tccgtcgcca ttgcgctctt tcaggcggcc tacatggcgg agatcgttcg
    16201 cggcggcctt cagacgattc cccgcggcca gttcgacgcg gccgcctcgc tcggcctttc
    16261 ccagcgacag gtctatacgg cggtgatcct gccgcaggcg ctcgtggcca tcattccggc
    16321 cttcgtcaac agcctgctct cgtgcttcat ggacacgtct ctggtgacgg tcgtctcgat
    16381 gtacgacctg acgggcagcc tgcgcctcgc tctgggtgac gcgctctggc gcggctactt
    16441 cattgagggc tatgtcttca tcgccctcgt gtacttcttc ttcagcttca tcgtgtcgcg
    16501 atacagccag tggctcgaag tctatctgac gggcgaaaag gaccgcggac acgccgccga
    16561 gtgatccccg gcgaaaccgg aaaatcaaaa aacaaaggca gacagcatgc gcaaggtaca
    16621 tgaacacatc atccaaatcc cgccgttcgt gacgcgggac cagctctgga acggtcttga
    16681 gaaggccgcc cgtcatcccg acaggtttgt tgagcacatg gagggctgcg
//
LOCUS       DBMDTI010000018        16104 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1155_length_16104_cov_3.578204, whole genome shotgun sequence.
ACCESSION   DBMDTI010000018 DBMDTI010000000
VERSION     DBMDTI010000018.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 16104)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16104)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16104
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1155_length_16104_cov_3.578204"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(37..642)
                     /locus_tag="ACZPB6_02015"
     CDS             complement(37..642)
                     /locus_tag="ACZPB6_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1183287.1"
                     /translation="MAGTPPIAHIQNILGPAPEDVIEWVHDRALHPDTNQRRSCDIDR
                     TPFEKWIYDNCQKLGLNLEKFAARHGVHSTTLGDYEVGKANISDVKLMELVRMYSVPE
                     AYEEWRKLRHLSGQDPRGHSGDLNDGLCRFGILLRTLRKELGVSQRELCRALGKDSKY
                     ICNLEYGYTRVTPEIILRIANYLNVPERIPDFFAAVDEDDN"
     gene            complement(880..1968)
                     /gene="mnmA"
                     /locus_tag="ACZPB6_02020"
     CDS             complement(880..1968)
                     /gene="mnmA"
                     /locus_tag="ACZPB6_02020"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR1183288.1"
                     /translation="MSKKTVVIGLSGGVDSSVSAWLLKEQGYNVIGLFMKNWEDDDDS
                     EYCSSRQDFIDASAVADVIGIDIEAVNFAKEYRERVFSDFLREYSAGRTPNPDVLCNA
                     EIKFRAFLDHAMAMGADLIATGHYARVRHTDDGVQLLRGVDPGKDQSYFLHRLTQEQI
                     SRVIFPVGEICKTEVRRIADDLKLPNAKKKDSTGICFIGERPFREFLNRYLPTQPGPI
                     RIPDGTVVGEHMGLSFYTLGQRKGIGVGGRHDSTGEPWFVAKKDLATNTLWICQGHDH
                     PWLLAHELKAVHPSWISGHAPELDAELTVKTRYRQPDDPCRLEYVSEKNFTLGFDKPQ
                     WAATPGQSAVLYQGDVCLGGGFIDTVKH"
     gene            2045..3133
                     /locus_tag="ACZPB6_02025"
     CDS             2045..3133
                     /locus_tag="ACZPB6_02025"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR1183289.1"
                     /translation="MSQTNESCTRFAKLLGIEVPFLNAPMEGIATPELVAAVSEAGGL
                     GVIPAAGLAPDEIRAFAARVKELTQKPFAVNLAVPVDAPADAAERFERFGDAVSMLLE
                     ELELPAGEGASYAERYDLEGCTRPDFFEQFDAALEVRPAAVISSFGGFREPEEEKLAE
                     LGIVNIGTATTLREAKVLRAAGCGAVIVQGAEAAGPRLSFEDPEDALVGLMSLVPAAV
                     RATGLPVIAAGGIASAEQVNALLAMGAAGVSVGTALLTTAESSACDAHRYYAEFGTAC
                     DTVLTRIYNGRLSRVLRNALVEALDDWELMTAGYPAQKALMGPLDRCASEVGRNDLVM
                     LPLGQSAGRSAYRRTADCVHALFPRRAD"
     gene            3412..4920
                     /locus_tag="ACZPB6_02030"
     CDS             3412..4920
                     /locus_tag="ACZPB6_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR1183290.1"
                     /translation="MTESSIEIGAMSNRGKLLALVAGPILGLLTYFLLPESYINSQGV
                     ETAFPYAGRACMGVTVWMAIWWFTEALPIAATAMIPLVVYPLLQITSAKAAMSNYASG
                     TIFLFLGGFLLAVGIHRWHLDRRIALLTLRLFGTQPKQMVLGLMLATAGLSAWVSNTA
                     TAAMMVPIAIAVLGVVRSTQKSEVIGKDERNFGIAVLLSIAYAASIGGMVTVIGSPPN
                     GIYVRFMEQTYGETVSFIDWLKIGLPVALLLLPATYFLLVNVLFPCKMEGIPGGKDWV
                     RNELRKLGAMSRGEKIVLAVFVLAAVLWIFGPVIRGLEFDGAKPFAALKDETIAMAAG
                     LLLFAIPVDSKRGIHALDWSSAKDVIAWDVLLLFGGGLTMAAALQSTGAAALVGAQAT
                     ALGSLGEVSMMAGVATLVTFATEVTSNTALAATMMPLISASADALKIDASGLLVTTAL
                     AASCAFMMPVATPPNAIVFGTGRLKIGDMVKAGFWLNILGVVIITCVAAVIL"
     gene            complement(4999..5730)
                     /locus_tag="ACZPB6_02035"
     CDS             complement(4999..5730)
                     /locus_tag="ACZPB6_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR1183291.1"
                     /translation="MQDILQQLSPILGNPALWLAVTLVAFIFGQHLYKLTQFNPLFTP
                     IIVAVVSIIVVLLVMGVPYEQYFQGAGVIHFLLGPATVVLAVPLFEQRQKLVELWLPL
                     TCGLLAGSVAAIVSVVAFGWLFGLSAETMISMIPKSVTTPIAMGVSEAMGGRPDLTAC
                     LVVITGIFGSIVGRPLFNLCRITSDPVRGVTLGLAAHGMGTSAAFQISNRAGAFGGLA
                     MGLSGIITAFLAPLIAGPLMKLLGI"
     gene            complement(5733..6104)
                     /locus_tag="ACZPB6_02040"
     CDS             complement(5733..6104)
                     /locus_tag="ACZPB6_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIR1183292.1"
                     /translation="MLSAIALILAFQVVGEVISRSAGIPVPGPVIGMILMLLAFFVKD
                     DLIGRIRPTGGVLLSNLSLLFVPAGVGVMRYGDLFLREGLAIGAVLVISTLIAMLVTA
                     YTIIFVDKLVHKNKNKNKNKD"
     gene            6358..6594
                     /locus_tag="ACZPB6_02045"
     CDS             6358..6594
                     /locus_tag="ACZPB6_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1183293.1"
                     /translation="MKQEFQIKGELITLDALLKAVGAADSGAHAKAMVAEGLVRVDGE
                     VELRKTRKLRGGERVELEGLTVVLKADPAFAARD"
     gene            complement(6670..8352)
                     /locus_tag="ACZPB6_02050"
     CDS             complement(6670..8352)
                     /locus_tag="ACZPB6_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR1183294.1"
                     /translation="MTQSFPWYKSYPAGVPHEINPDRYPSIPAVLDDIAARYPDKVGY
                     TNMDCGLTYSRTAALTKELAAYLQSLGLKPGDRVAIMMPNLLQYVIAMYACLRAGYIV
                     VNINPLYTSREVNGTLRDSEAKAIIIIENFAKTLQNALPGTKCEHIITTAVGDLFPFF
                     KRTLVNFVVRKVKKMVPEYSLPNAVGFNEALEIGRRHGFTAHEIHNTDIAFLQYTGGT
                     TGVAKGAILTHRNLLANLQQVGYWLASACKEGEEVALTALPLYHIFSLTATLSFTQFA
                     ANMVLITNPRDIDGLAKECAKWDFSIIVGVNTLFTAMLNNKIFCAHKFTRLKVTAGGG
                     SQVQRTIAERWKQQTGCNILEAYGLTECSPGVCGNIPEAPWDGSVGVPIPSTEVSIRG
                     EGFRDLGCCPSPDKIPEYTGEICVRGPQVMQGYWNKPEETAAVLRDGWLRTGDVGHMD
                     MNGSITITDRKKDMILVSGFNVYPNEVENVIASMPGVLEVGVVGVPSAKSGETVKAVI
                     VRKDPALTADDVKKYCRTQLTGYKMPREIIFVDSLPKTAVGKILRRELKDIQ"
     gene            complement(8605..9144)
                     /gene="hemG"
                     /locus_tag="ACZPB6_02055"
     CDS             complement(8605..9144)
                     /gene="hemG"
                     /locus_tag="ACZPB6_02055"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HIR1183295.1"
                     /translation="MKTVLVVYDSRSGHTARIARRIWETIIAEGHQADMMHVLEADRE
                     GVDWNKYDLVICGAPVLYGVFRKQFLAFVNRWKAVLDAKPNSFFNVTVIARNPAKATP
                     EGNVYCRKFLENNPWHPKDVKCFAGKVDYPNWSWIDAKLIQMIMKMTKGPTEMTAVID
                     YTDWEAVEDYARHCLTLEA"
     gene            9466..9729
                     /gene="infA"
                     /locus_tag="ACZPB6_02060"
     CDS             9466..9729
                     /gene="infA"
                     /locus_tag="ACZPB6_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR1183296.1"
                     /translation="MAKEDLIEMSGQVLEVLPDARYRVKLDNGHELVAYTNGKMRKHH
                     IRILAGDKVSLELSPYDLTKGRITFRHIEGRPPAGAQNGNRRR"
     gene            complement(9832..10803)
                     /locus_tag="ACZPB6_02065"
     CDS             complement(9832..10803)
                     /locus_tag="ACZPB6_02065"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR1183297.1"
                     /translation="MRLGFTIQEAYGILLVGLALLAAAGTFYANWALNRSSDPEHRAR
                     LLAVNARVRMSWWLIVVFTIAFALGQATLLVFFALISFFLLREFIAITPTKPTDHNAL
                     VVAFYIAIPVQYALIGFDLSYFTTLFIPTYLFLALPVIMAMTKDTERYLERVAKVQWG
                     VMLCVFCVSHAPAIATLDLTRYNSSGPLLLLFFLLVVFVSDLASSIASSFFGGKTLSF
                     NSNRTIKGTLIGSFCGVLAGAAMFWITPFRAWQAVIMAVIIAASCVMGDLVINSVRHS
                     MGATRLAGEGDIYMTRGTLARLAPLTFAAPVFYHLTITFFILYKDAF"
     gene            complement(10800..11414)
                     /locus_tag="ACZPB6_02070"
     CDS             complement(10800..11414)
                     /locus_tag="ACZPB6_02070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1183298.1"
                     /translation="MFKSFKLMTPIVWLTRLLVGAYPHWQGCAPSSKQRIYFANHTSH
                     LDTIVIWASLPPQLRPFVRPVAAKDYWEKGLLRRRIALDELNVVLVDRRRTEHSNPLD
                     PLRQALQEGSSLIIFPEGTRRPQPLPSEFKSGIWRLMKEFPQVELIPVYIENLHRAMP
                     KGVLLPVPTVCSIRFGAPLEHSPEDLKEDFLERARNAIISLAQP"
     gene            complement(11470..12951)
                     /gene="tldD"
                     /locus_tag="ACZPB6_02075"
     CDS             complement(11470..12951)
                     /gene="tldD"
                     /locus_tag="ACZPB6_02075"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIR1183299.1"
                     /translation="MTTFTSSLERLSAAQALFYSGTGLDDGALLKVLGRMTPAGADWA
                     DIYLQQTVSRSWILDEGAVKTGGFSIDRGAGLRTVCGETSTLAYTDVLTPVSLLDAAG
                     TVVASAAYFPEGAPGVVIPACLTPGSYRPCCGTEDASSNMHTPQALELIREADALARE
                     ADPRVTDVVVTLQCETDATLLCRLDGLLSGDIRPMVYLSISVIASENGRRERASAGGG
                     ARAGLEFFTRERIRKWARDAVSEAIHNLSAAPAPAGIMPVVLGPGWPGILLHEAVGHG
                     LEGDFIRKGTSAFTGRIGMRVASPGVTVVDDGTIGLARGSICADDEGTPAARTTLIED
                     GILTGYMHDLTSARVLGTAPTGNGRRESFATLPLPRMTNTFMTPGDCDPADIVKSVKR
                     GIYASNFSGGQVDITNGKFVFAMSRAWMIEDGRLTHPVRGAMLSGSGPEALKHIPLIG
                     NDLALDEGTGQCGKDGQQVPVGVGMPTIRIDALTVGGTAENAV"
     gene            complement(12956..13765)
                     /locus_tag="ACZPB6_02080"
     CDS             complement(12956..13765)
                     /locus_tag="ACZPB6_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012993.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR1183300.1"
                     /translation="MRISACQMISGIDPEQNVLDAVNLISASADSGADLVVLPENFAL
                     MAGDAANLGIAEAVGEGPIQDAVRMAAMRCGLWVVAGSIPLKGKDATHYTQSSLVFDP
                     EGNIAARYDRIHLFRLQNKTEFYDERIYIEPGKSPVTFRMTTWDGESFSVGLSLGFDL
                     RFPELFRGLSQPDLIVLPATFTEMTGRAHWATLLAARAIENQCFVAAAAQGGHHACGR
                     RTWGHSRIIDAWGGMVSELASGTGIVIGNIERSQLENVRSTLPALASRTIY"
     gene            complement(13815..>16104)
                     /locus_tag="ACZPB6_02085"
     CDS             complement(13815..>16104)
                     /locus_tag="ACZPB6_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YhdP family phospholipid transporter"
                     /protein_id="HIR1183301.1"
                     /translation="GKHKNDGHFAIEADMTGGRLDFLPSHVRKADGSWETASSWPVLN
                     NIDAHLAFIGNGFEIRGRSATSHDLKASDVVVSMKSYTDADLEITGRAQGDLGRVLRY
                     LNQGRMLNDILSSAFAESKGSGAADVRMNLHIPLSGDIVRNLRVNIDADIRNATFYYG
                     LNLPTVSGVNGSLHITEKSVVTPTPLTGTSPGGKPVKVTAETKDGVMTLGIDAEPTTA
                     ELEHFLGIDVAELFFKKLTGTSPVHVDAEIGLTKSRFAVTGRTDLDGIASTLPEPYEK
                     AAGEKWPTTFSVTLPEKAMHVDVSSPGRAAVALRFTRDKDGLSLRHGYAGLGSAPMRA
                     PAEGIAIRVETPRASIDDWQPYILQDESPAKPGSGRTAAGESAVDRISMVEAEIGGAL
                     WSDKEFRNLSLTARRFAKNDWHVRVAGDDAAGQIEYNQATSEAHARLKVNLTRFALPD
                     SKAESFVPSARQEAVTTVSDLPDISLVIDDLKVGKRHLGKVEFEASRRVEAGNIVWRI
                     TEAALRNAGSVIRARGSWVHTPGKSGETSVSIDGNILDAGKLLASLDIPHAVNGAPTT
                     LHADLSWNNAPHRPDLSTLRGSFSSEMKRGEFLQIEPGAGRLLSLLSMQHLLRRLTLD
                     FRDVLGKGFAFDSAHVAGTVKNGVLSIPKASVLGSSATVMVGGSISLVRETLDLDAII
                     LPAINAGGPALALSIINPAVGIGTFLTQWVLKDQLSNMFRMEYEIKGPFDDPVVEKAS
                     RTELGAEIKPAAPGERIRPLFD"
BASE COUNT         2817 a         4661 c         5525 g         3101 t
ORIGIN      
        1 gctcagatga ctgtcgtcga ggccctgctc ggtcgcctag ttgtcgtctt cgtcaaccgc
       61 agcgaagaaa tcgggaattc tctcgggcac gttcagatag ttggcgatcc gcaggatgat
      121 ctccggggtg acacgggtgt atccatattc aaggttgcag atgtacttgc tgtccttgcc
      181 gagcgcgcga cacaattctc tttgactcac tcccagttcc tttcgcagtg tgcgcaggag
      241 gatgccgaag cggcagaggc cgtcattgag gtctcctgaa tggcctcgag gatcctgtcc
      301 ggagagatgg cgcagtttgc gccattcttc gtaggcttcg ggtacgctgt acatccggac
      361 cagctccatg agcttgacgt ccgaaatgtt tgcctttcca acctcgtagt ccccgagggt
      421 ggtggaatgc acgccatgtc tggcagcgaa cttctcaagg ttgaggccga gcttttggca
      481 gttgtcgtaa atccactttt cgaaaggtgt acggtcgata tcgcatgacc tgcgctggtt
      541 ggtgtcgggg tggagcgctc ggtcgtgcac ccattcgatc acgtcttcag gcgcgggacc
      601 gaggatgttc tgaatgtgcg cgatgggggg cgtaccggcc aatctcgtat tccctgaggg
      661 tggtggagtt gatgccaagc ttgtcggcga ggtgctggcg gacaccgccg tgtttcttcc
      721 tttcgtggca cagccattgc gccagcggat tgaggtcggt tgtctgcatg tgatggttgg
      781 gtcgtggttg gaattggttt gaggggcggc tgtcgtgccc tgcctgcggc cgccatgtct
      841 gtcgagcgtg tgactcatgg cggccgggac ggcgacgggt cagtgcttca cggtgtcgat
      901 gaagccgccg ccgaggcaga cgtcgccctg atagagaacg gcgctctggc cgggcgtggc
      961 cgcccactga ggcttgtcga agccaagcgt gaagttcttt tcggagacgt actcgagtct
     1021 gcagggatcg tcgggctggc gatagcgggt cttcaccgta agctcggcgt caagctcggg
     1081 cgcatgtccg gagatccagc ttggatggac ggccttgagc tcgtgcgcga gaagccacgg
     1141 gtgatcgtgt ccctggcaga tccagagcgt attggtggca agatccttct tcgcgacgaa
     1201 ccacggctcg cccgtggagt cgtggcggcc gcccacaccg atgcccttgc gctggccgag
     1261 cgtatagaag gaaaggccca tgtgctcgcc cacgaccgtg ccgtcgggaa tgcggatcgg
     1321 accgggctgg gtaggcagat agcggttgag gaactcgcgg aacggacgct cgccgatgaa
     1381 gcagatgccc gtggagtcct tcttcttcgc attgggaagc ttgaggtcgt cggcgatgcg
     1441 gcggacctcg gtcttgcaga tttcgcccac ggggaagatc acgcgggaga tctgctcttg
     1501 ggtgaggcgg tgcaggaagt agctctggtc cttgccggga tcgacgccgc gcagcagctg
     1561 gacgccgtcg tccgtgtgac ggacgcgggc gtagtgcccc gtggcgatga ggtcggctcc
     1621 catggccatg gcatggtcga ggaaggcgcg gaacttgatc tctgcgttgc agagaacgtc
     1681 gggattgggc gtgcggcctg cgctgtattc gcgtagaaag tcggagaaga cgcgctcgcg
     1741 gtattccttc gcgaagttga cggcctcgat gtcgatgccg atcacgtcgg cgacggcgct
     1801 cgcgtcgatg aagtcctgac gggaggagca gtattcgctg tcatcgtcgt cctcccagtt
     1861 cttcatgaag aggccgatga cgttgtagcc ttgctccttg aggagccagg cgctgacaga
     1921 ggagtcgacg ccgcccgaaa ggccgatgac gacggttttc ttggacatac gtatatatgg
     1981 tcggtaagtc gggatagaat cgggacgtac ccttgaattc attaattagg agatcgtggc
     2041 aattatgtct cagacgaacg aatcgtgcac ccgctttgcg aaacttcttg gcatcgaggt
     2101 gcccttcctc aatgctccga tggagggcat tgccacgcct gagctcgtgg cggccgtgtc
     2161 cgaggcgggc ggtctcggcg tcattcccgc cgccggtctc gctcccgacg agatccgcgc
     2221 ctttgccgcg cgcgtgaagg agctcacgca gaagcccttc gccgtgaacc ttgctgtgcc
     2281 cgtcgacgcg cccgcggatg cggcggaacg cttcgagcgt ttcggcgacg ccgtctcgat
     2341 gctgctcgag gagctcgagc tgcccgcagg cgaaggcgcc tcctatgccg aacgctacga
     2401 tctcgagggc tgcacccgcc ccgacttctt cgagcagttc gatgcggcgc tcgaggtgag
     2461 gcccgcagcc gtcatctcgt ccttcggcgg cttccgcgag cccgaggagg agaagctcgc
     2521 cgaactcggc atcgtcaaca tcggcaccgc cacgacgctc cgtgaggcca aggtcctgcg
     2581 cgccgcgggc tgcggcgccg tcatcgttca gggagcggag gccgcgggcc cgcgcctctc
     2641 gtttgaggat cccgaggacg ctctcgtcgg tctcatgtcc ctcgtgcccg ccgccgtccg
     2701 cgcgacgggg ctgcccgtca tcgcggcggg cggcatcgcc tcggccgagc aggtgaacgc
     2761 acttctggcc atgggcgctg caggcgtgtc cgtcggcacg gccctgctga cgacggccga
     2821 gtccagcgcc tgcgacgcgc accgctacta cgcggagttc ggcacggcct gcgacaccgt
     2881 actcacgcgc atctacaacg gcaggctcag ccgcgtgctc agaaacgcgc tcgtcgaggc
     2941 cctcgacgac tgggagctca tgacggccgg ctaccccgct cagaaggccc tgatgggccc
     3001 ccttgacagg tgtgcctccg aagtcggacg caacgacctc gtcatgctgc ctctgggaca
     3061 gtccgcaggc cgcagcgcct acaggcggac tgccgactgc gtgcatgcgc tctttccccg
     3121 tcgcgcagac tgaatcaaac aaacggatag attttcaaaa tcgtcgcaac accttgaaaa
     3181 tcgacgtacg gcgtccgact taggtaaaac ccctcggact tgggggttaa tttcacaatg
     3241 cggaacgcgc tttcgtattg tgaaatatgt gacctggttt tgcgggttat cccttgaata
     3301 tgtgtcgttg aaaagggttt cgggcttgat tactatcaac cgaacagaac tcgtttacga
     3361 tttcacggat cggtaggcgg gattcgatca acattaactg agggttcaac catgaccgaa
     3421 tccagtattg aaatcggcgc catgtccaac aggggcaagc ttctggcgct cgtcgccggc
     3481 ccgatcttgg ggctgctgac ctacttcctt ctgcctgaaa gctacatcaa ctcccagggc
     3541 gttgaaaccg ccttcccgta tgcgggccgc gcctgcatgg gcgtgaccgt gtggatggcg
     3601 atctggtggt ttaccgaagc gcttccgatt gcggcgaccg caatgattcc actggtcgtg
     3661 tatccgcttc tgcagatcac gtccgccaag gctgcgatga gcaactacgc ctccgggacg
     3721 atcttcctct tcctgggcgg cttcctgctc gcggtcggca tccaccgctg gcaccttgac
     3781 cgccgcatcg cactgctgac gctgcgcctc ttcggcaccc agcccaagca gatggtgctc
     3841 ggcctgatgc tggccactgc cggcctctcc gcctgggtgt ccaatacggc gactgccgcc
     3901 atgatggttc ccattgccat cgccgtgctc ggcgtcgtgc gctccaccca gaagagcgaa
     3961 gtcatcggca aggatgaacg caacttcggc atcgccgtgc tgctctccat cgcctacgcg
     4021 gcctccatcg gcggcatggt caccgtgatc ggctcgcccc cgaacggcat ctacgtccgc
     4081 ttcatggaac agacctacgg cgagaccgtg agcttcattg actggctcaa gatcggcctt
     4141 ccggttgcgc tcctgctgct cccagccacc tatttcttgc tagtgaacgt tctcttcccc
     4201 tgcaagatgg aaggcattcc cggcggcaag gactgggtcc gcaacgagct tcgcaagctc
     4261 ggtgccatgt cccgcggtga aaagatcgtt ctggcggtct tcgtcctcgc ggccgtcctc
     4321 tggatcttcg gcccggtcat ccgcggcctt gaattcgacg gcgccaagcc cttcgcggcc
     4381 ctcaaggacg aaaccatcgc catggctgca ggcctcctgc tcttcgcgat ccccgtcgac
     4441 tccaagcgcg gcatccacgc tcttgactgg tcctcggcca aggacgtgat cgcttgggat
     4501 gtgctgctcc tcttcggcgg cggtctcacg atggcggccg ctctgcagag cactggcgcc
     4561 gctgccttgg tgggcgctca ggccacggcc ctcggcagcc tcggtgaagt ctccatgatg
     4621 gccggcgtcg ctaccctcgt gaccttcgct accgaagtga cctcgaacac cgcacttgcc
     4681 gccacgatga tgccgctcat ctccgcctca gccgatgcgc tcaagatcga tgcctccggc
     4741 ctcctcgtga ccaccgcgct cgccgcttcc tgtgccttta tgatgcccgt cgccacgccg
     4801 cccaacgcga tcgtgttcgg caccggccgt ctgaagatcg gagacatggt gaaggctggc
     4861 ttctggctca acatcctcgg cgtcgtgatc atcacctgcg tggccgcagt aatcctctga
     4921 ccgcctctct gaaaaacaag cgaacaagcg cgcacccccg aaaggaggcg cgcgctttca
     4981 tttgaggtgc aggcaggctc agatgcccag aagcttcatg agggggcctg cgatgagcgg
     5041 cgcaaggaag gcggtgatga tgccggagag gcccatggcc agaccgccga aggcgcccgc
     5101 cctgttcgag atctggaatg cggcgctcgt gcccatgccg tgtgcggcga ggccgagcgt
     5161 cacgccgcgc actgggtcgg acgtgatgcg gcagaggttg aagagcggac ggccgacgat
     5221 ggagccgaag atgcccgtga tcacgacgag gcaggccgtc aggtcggggc gcccgcccat
     5281 ggcttcggac acgcccatgg cgatcggtgt cgtgacggac ttcggaatca tggagatcat
     5341 tgtttcggcc gagagtccga agagccagcc gaaggcaacg acggagacga tggcggctac
     5401 gctgcctgcg agaaggccgc aggtgagggg cagccagagc tcgacgagct tctggcgctg
     5461 ctcgaagagc ggcacagcga gcaccacggt cgcgggcccg agcaggaagt gaatgacgcc
     5521 tgcgccctgg aagtactgct cgtagggcac gcccatgacg agcagcacga cgatgatgct
     5581 cacgacggcc acgatgatcg gcgtgaagag cggattgaac tgcgtgagct tgtagagatg
     5641 ctggccgaag ataaaggcga cgagcgtgac ggcaagccag agggcgggat tgccgaggat
     5701 cggcgagagc tgctgaagaa tgtcctgcat ggtcagtcct tgttcttgtt cttgttcttg
     5761 ttcttgtgca cgagcttgtc gacgaagatg atggtgtagg cggtcacgag catggcgatg
     5821 agggttgaga tcacaagcac ggcgccgatg gcgaggccct cgcgaaggaa aaggtcgccg
     5881 tagcgcatga cgcccacgcc cgccggcacg aagagaagcg acaggttcga taggagcacg
     5941 ccgcccgttg ggcggatgcg gccgatgaga tcgtccttga cgaaaaaggc gagaagcatg
     6001 aggatcatgc cgatcacggg acccggaacg ggaatgcccg cgctgcgact gatgacttcg
     6061 ccgacgacct gaaaggccag aatgagtgcg atggcggaaa gcatgaaaga agtaccgtaa
     6121 aatgttctga ccgtctttcg gcgtgctagg gtttgcacgt atgaaaggtg tcaaggtgtt
     6181 gattgtgtca ctacggcgcc tgtcgtgggg gcattcggat ttgacttagg ttaggaaagt
     6241 tgaagtgtcg gcggttcttg tcttcagaag aggaccgctc cgacgggctg cctggccgaa
     6301 cttgaagagg aggacggcgc gtcctctccc gtcccgggga ctgcgcgccg aatttctatg
     6361 aagcaggaat ttcagatcaa gggcgagctc atcacgctcg acgcccttct caaggctgtc
     6421 ggcgcggccg actccggcgc tcatgccaag gcgatggtcg ccgagggcct cgtgcgcgtc
     6481 gacggcgagg tcgagcttcg caagacgagg aagctccgcg gcggcgagcg cgtggagctc
     6541 gagggcctga ccgttgtgct caaggccgat ccggcctttg ccgcacggga ctgagtccgc
     6601 acgtcaagat aatgcaaaaa tgccggcttc cgcaggaaac tggcattttt tcatggggct
     6661 gcagcagaat tattggatgt ccttgagctc gcggcgcagg atcttgccca cggcggtctt
     6721 cgggagcgag tcgacgaaga tgatctcgcg cggcatcttg tagcccgtga gctgggtgcg
     6781 gcagtacttc ttgacgtcgt cggcggtgag ggcgggatcc ttgcgcacga tgacggcctt
     6841 gacggtttcg ccggacttgg cgctcggaac gcccacgacg cccacttcaa gcacgccggg
     6901 catgctcgcg atcacgtttt cgacttcgtt cggatagacg ttgaagccgg agaccagaat
     6961 catgtccttc ttgcggtccg tgatcgtgat gctgccgttc atgtccatgt ggcccacgtc
     7021 gcccgtgcgc agccagccgt cgcgaagaac ggcggccgtc tcctcgggct tgttccagta
     7081 gccctgcatg acctgcgggc cgcgcacgca gatctcgcct gtgtattcgg gaatcttgtc
     7141 gggcgacggg caacagccga ggtcgcggaa gccttcgccg cggatggata cttcggtcga
     7201 cgggatgggc acgcccaccg agccgtccca cggagcctcg gggatgttgc cgcagacgcc
     7261 gggcgagcat tccgtgagac cgtaggcctc gaggatgttg cagcccgtct gttgcttcca
     7321 gcgttcggcg atggtgcgct gcacctggga gccgccgccc gcggtgacct tgaggcgcgt
     7381 gaacttgtgg gcgcagaaga tcttgttgtt gagcatcgcg gtgaagagcg tgttgacgcc
     7441 gacgatgatg gagaagtccc acttggcgca ttccttggcc agcccgtcga tgtcgcgcgg
     7501 gttggtgatg agcaccatgt tcgcggcgaa ctgcgtgaag gagagcgttg ccgtgaggga
     7561 gaagatgtgg tagagcggca gcgccgtgag ggcgacttcc tcgccttcct tgcaggcgct
     7621 cgcaagccag tagcccacct gctggaggtt ggcgaggagg ttgcggtgcg tgaggatggc
     7681 gcccttggca acgccggtcg tgccgcccgt gtactgcagg aaggcgatgt cggtgttgtg
     7741 gatctcatgg gcggtgaagc cgtggcggcg gccgatctca agcgcttcgt tgaagccgac
     7801 ggcgttcggg agcgagtatt cgggcaccat cttcttcacc ttgcgcacga cgaagttgac
     7861 gagcgtgcgc ttgaagaacg ggaagaggtc gccgacagcg gtcgtgatga tgtgctcgca
     7921 cttcgtgccg ggcagggcgt tctggagtgt cttggcgaag ttctcgatga tgatgatcgc
     7981 cttcgcctcg gagtcgcgca gcgtgccgtt gacctcgcgg gaggtgtaga gcgggttgat
     8041 gttgacgacg atgtagcccg cgcgcaggca ggcgtacatg gcgatgacgt actgcagaag
     8101 gttcggcatc atgatggcga cgcggtcgcc gggcttgagg ccgagactct ggaggtaggc
     8161 ggcgagttcc ttggtgagcg cagccgtgcg gctgtaggtc aggccgcagt ccatgttggt
     8221 gtaccccacc ttgtcggggt agcgcgcggc gatgtcgtcg aggacggccg gaatgctcgg
     8281 atagcggtcc ggattgattt cgtgcgggac gcctgcgggg tagctcttgt accagggaaa
     8341 gctttgagtc atgtcttagt gatcttcgaa ttcttgcgat ggtggccggc ctgagccggc
     8401 ggtgcggatg cgcccgaagg aaggcttggc tcccatggcc cgcgccgttg atgaacatcg
     8461 gcgaaaggcg gacaagcgca cctagcttaa gtttagcggc acgaggacgc gaaaacgagg
     8521 tcaaaaaaca cgacttaacc cctatatgcg caaaaagcct ccggattccg gaggcttttc
     8581 gtgtgcggaa cctgagcgga aggcttaggc ttcgagcgtc aggcagtggc gggcgtaatc
     8641 ttcaacggct tcccagtcgg tgtagtcgat gacggccgtc atttccgtcg ggcccttggt
     8701 catcttcatg atcatctgga tgagcttggc gtcgatccag ctccagttcg ggtagtcgac
     8761 tttgccggcg aagcacttga cgtccttcgg gtgccacggg ttgttctcaa ggaacttgcg
     8821 gcagtagacg ttgccttcgg gcgtggcctt ggccgggttg cgggcgatga cggtcacgtt
     8881 gaagaagctg ttgggcttgg cgtcgaggac ggccttccaa cggttgacga aggcgaggaa
     8941 ctgcttgcgg aacacgccgt agaggacggg agcgccgcag atgacaaggt cgtacttgtt
     9001 ccagtcgacg ccttcgcggt cggcttcaag cacgtgcatc atgtcggcct ggtggccttc
     9061 ggcaatgatg gtttcccaaa tgcggcgggc gatacgagcg gtatggccgg agcggctgtc
     9121 gtaaacaaca agcaccgtct tcatgttcta ttctccaaaa gggtcgccgg cgcgcaggcg
     9181 agcgcacgcc gaaaaatgtg tttcaagagc agggctttcg caggtcttcc ctactcccgc
     9241 ctgtatttta gccaagtgta tattgtccga gccttaaccg tatggggaga aacgacgaag
     9301 attgcgggta aaacccgatg ttgcaaggac aatcagtgct acaatcacgc acttgtgacg
     9361 gcgaccgttc gccgtcgata gtttcaccca tcgccgtgtc cgcctgctgc ccgacgtgca
     9421 gccttttttt gtttttgagg acccggcgga tcaacttgga agaatatggc aaaggaagat
     9481 ctcatcgaga tgtcgggcca ggtgcttgaa gtgctgcccg atgcccgtta tcgcgtcaaa
     9541 cttgacaacg gtcatgaact ggtggcctac accaacggca agatgcgcaa gcatcacatc
     9601 cgcattctcg cgggcgacaa ggtgtccctc gagctctcgc cgtacgatct gaccaagggc
     9661 cgcatcacct tccgtcacat cgaaggccgt ccgcccgccg gtgcccagaa cggcaaccgc
     9721 cgtcgctaag aggcccggtc gtcagaccgc gtgaaaaaat gaaaaacccc cgaagccgca
     9781 tggcccgggg gttttctttt ggtcgttcct tccggacgtc cgtccttctg cttagaacgc
     9841 gtccttgtag aggatgaaga atgtgatcgt cagatggtag aagacggggg ccgcgaaggt
     9901 gaggggagcg aggcgcgcaa gcgtgccgcg cgtcatgtag atgtctcctt cgcctgcaag
     9961 gcgcgtggcg cccatgctgt ggcgcacgga gttgatgacc aggtcgccca tcacgcagga
    10021 ggccgcgatg atgacggcca tgatgacggc ctgccaggcg cggaacggcg tgatccagaa
    10081 catggccgca ccggcgagca cgccgcagaa ggagccgatg agcgtgccct tgatcgtgcg
    10141 gttggagttg aagctcaggg tcttgccgcc gaagaaggag ctggcgatgc tcgaggcgag
    10201 gtcgctcacg aatacgacga gcaggaagaa gaggagcagc agcggaccgg acgagttgta
    10261 gcgcgttagg tcgagcgtcg cgatggcggg tgcgtggctc acgcagaaga cgcagagcat
    10321 gacgccccac tgcaccttgg cgacgcgctc gagatagcgc tccgtgtcct tcgtcatggc
    10381 catgatgacg ggcagcgcga ggaacaggta cgtcggaatg aagagcgtcg tgaagtacga
    10441 gaggtcgaag ccgatgagcg cgtactgcac gggaatcgcg atgtagaacg cgacgacgag
    10501 cgcattgtgg tcggtgggct tcgtaggcgt gatcgcgatg aactccctca gcaggaagaa
    10561 gctgatgagc gcgaagaaga cgagaagcgt ggcctggccg agcgcgaagg cgatcgtgaa
    10621 gacgacgatg agccaccagg acatgcgcac gcgggcattg accgcaagca ggcgggcgcg
    10681 atgttcggga tcgctgctgc ggttcagggc ccagttggcg tagaaggtgc cggcggcggc
    10741 aagaagcgcg aggccgacga gaaggatgcc gtaggcttcc tgaatggtga atccgagtct
    10801 catggctgtg ctagggagat gatggcgttg cgggcgcgtt caaggaaatc ctccttcagg
    10861 tcttcgggag aatgctcgag cggggcgccg aagcggatgg agcagacggt gggaacggga
    10921 agcagcacgc ccttgggcat ggcgcggtgc aggttctcga tgtagacggg aatcagctcg
    10981 acctgaggaa attccttcat caggcgccag atgccggact tgaactcgct cggcagcggt
    11041 tggggacggc gggtgccctc ggggaagatg atgagggagg agccttcctg tagcgcctga
    11101 cgcaggggat cgagcggatt gctgtgttcc gtgcggcggc ggtcgacgag cacgacgttg
    11161 agctcgtcaa gcgcgatgcg gcggcgaagc aggccctttt cccagtagtc cttggcggcg
    11221 acggggcgaa cgaatggacg gagctgcggg ggcagcgagg cccagatcac gatcgtgtcg
    11281 agatggctcg tgtggttggc gaagtagatg cgctgtttgg aggacggcgc acagccctgc
    11341 cagtgggggt aggcgccgac gagcagcctc gtgagccaca cgatgggcgt cattagcttg
    11401 aacgatttga acacgatatg ttcctgtttt aagggaatct aaacattata aaccgcacgg
    11461 ctggtacgct taaacggcat tttccgcagt gccgccaacg gtcagcgcat cgatgcggat
    11521 ggtgggcatg cctacgccca cgggtacctg ctggccgtcc ttgccgcact gtcccgtgcc
    11581 ctcgtcaagt gcaagatcgt tgccgatgag cgggatgtgc ttgagggcct cgggacccga
    11641 gcccgatagc atggcgccgc gcacggggtg agtgagcctg ccgtcctcga tcatccaggc
    11701 gcggctcatc gcaaagacga acttgccgtt ggtgatgtcg acctgcccgc cgctgaagtt
    11761 gctcgcgtag atgcctcgtt tgaccgactt gacgatgtcg gcgggatcgc agtccccggg
    11821 cgtcatgaag gtgttggtca tgcgcggcag gggcagcgtg gcgaagctct cgcgtcggcc
    11881 gttgcccgtc ggggcggttc cgagaacgcg ggcgctcgtg aggtcgtgca tgtagcccgt
    11941 gaggatgccg tcttcgatga gcgtcgtgcg cgcggcgggc gtgccttcgt cgtcggcgca
    12001 gatggagccg cgcgctagtc cgatcgtgcc gtcgtcgacg accgtgacgc cgggggatgc
    12061 gacgcgcatg ccgatcctgc ccgtgaaggc ggaggtgccc tttctgatga agtcgccttc
    12121 aagtccgtgg cccacggcct cgtggagcag aatgccgggc cagcccggac cgagcacgac
    12181 gggcatgatg cctgcaggtg cgggtgcggc gctgagattg tggattgcct ccgagacggc
    12241 gtcgcgagcc cacttacgga tgcgctcccg cgtgaagaac tcaagccccg cgcgtgcgcc
    12301 gcctccggcc gaggctcgct cgcgcctgcc gttttccgag gcgatgaccg agatcgaaag
    12361 gtagaccatg gggcggatgt cgcccgaaag caggccgtca aggcggcaga ggagcgttgc
    12421 atccgtctcg cactgaaggg tgacgaccac gtctgtgacc ctgggatccg cctcgcgcgc
    12481 cagggcgtcg gcctcgcgga tgagctcaag cgcctgtggc gtgtgcatgt tcgaggatgc
    12541 gtcctccgtc ccgcagcagg gcctgtagga gccgggagtg aggcaggcgg ggatgacgac
    12601 gcctggggcg ccctcgggga agtaggcggc cgaggcgacc acggtgcctg cagcatcgag
    12661 aagcgagacg ggcgtgagca cgtccgtgta ggcgagcgtg gaggtctcgc cgcagacggt
    12721 gcgcagtcct gcgcccctgt cgatggagaa tccgcccgtc ttgacggcgc cctcgtcgag
    12781 aatccaggaa cgggagacgg tctgctgaag gtagatgtcc gcccagtccg cgcctgccgg
    12841 cgtcatgcgg ccgagcacct tgagaagcgc gccgtcgtcg agtcccgtgc ccgagtaaaa
    12901 aagcgcttga gcggctgaaa gccgctcaag cgaggatgtg aatgtcgtca tgtgttcagt
    12961 agatcgtgcg cgaggcgagc gcgggaagcg tgcttcggac gttctcgagc tggctgcgct
    13021 cgatgttgcc gatcacgatg cccgtcccgg aggcgagctc gctcaccatg ccgccccagg
    13081 cgtcgatgat tctggagtgg ccccaggtgc gcctgccgca ggcatggtgt ccgccttggg
    13141 cggccgctgc gacgaagcac tggttctcga tggcgcgtgc agcgagcagc gtcgcccagt
    13201 gggcgcggcc cgtcatttcc gtgaaggtcg cgggcaacac gatgaggtcg ggctgggaaa
    13261 ggccgcggaa gagttcggga aagcgcaggt cgaagccgag cgagaggccg acggagaagg
    13321 actcgccgtc ccaggtcgtc atcctgaagg tgacggggga cttgccgggt tcgatgtaga
    13381 tgcgctcgtc gtagaactcg gtcttgttct gcaagcggaa gagatggatg cggtcgtagc
    13441 gggccgcgat gttgccttcc ggatcgaaca cgagcgagga ctgcgtgtag tgggtggcgt
    13501 cctttccctt gagcggaatc gagccagcga cgacccagag gccgcagcgc atggccgcca
    13561 tgcgcacggc atcctggatc gggccttcgc cgacggcttc ggcaatgccg aggttggcgg
    13621 cgtcgcccgc catcagggcg aagttctcgg ggagcacgac gagatcggcg cccgagtcgg
    13681 cggaggccga aatgaggttg acggcgtcga gaacgttctg ttcgggatca atgcccgaaa
    13741 tcatctggca ggcggaaatg cgcatggtgt caaaagaaag ggatgttgtg tccgcacatt
    13801 gtagcggatg cgttctagtc gaaaagcggg cggatgcgct cgccgggcgc ggcgggcttg
    13861 atttccgcgc cgagttctgt tcgcgaggcc ttctcgacga cggggtcgtc gaacgggccc
    13921 ttgatctcgt actccatgcg gaacatgttc gagagctggt ccttgagaac ccactgcgtg
    13981 aggaaggtgc cgatgccgac ggcggggttg atgatcgaga gcgcaagcgc cggcccgccc
    14041 gcgttgatgg cggggagaat gatggcgtcg aggtcgagcg tctccctgac gaggctgatg
    14101 ctgccgccca ccatcacggt ggcggacgag ccgaggacgg aggccttcgg aatgctcagg
    14161 acgccgttct tcaccgtgcc cgcaacgtgg gcggagtcga atgcgaagcc cttgccgagc
    14221 acgtccctga agtcgagcgt gagcctgcgc agaaggtgct gcatcgacag caggctcagc
    14281 aggcggcctg cgccgggctc gatctgcagg aattcgccgc gcttcatctc acttgagaag
    14341 gagcccctga gcgtggagag gtcggggcgg tgaggcgcgt tgttccagga gaggtcggcg
    14401 tgcagggtcg tcggcgcgcc gttcacggcg tgcggaatgt cgaggctcgc cagaagcttg
    14461 cccgcgtcca gaatgttgcc gtcgatcgag acggaggtct cgcccgactt gccgggcgtg
    14521 tgcacccagc tacctcgtgc gcggatgacg ctgcccgcat tgcgaagggc cgcctcggtg
    14581 atgcgccaga cgatgttgcc cgcctcgacc cttcggctgg cctcgaactc aaccttgccg
    14641 aggtgacgct tgcccacctt gaggtcgtcg atgacgagtg agatgtcggg caggtcggag
    14701 acggtcgtga cggcctcctg cctggcgctc ggcacgaagc tctcggcctt gctgtcggga
    14761 agcgcgaagc gggtgaggtt gaccttgagg cgtgcatggg cctcgctcgt cgcctggttg
    14821 tattcgatct gtcctgcggc gtcgtcgccc gccacccgga cgtgccagtc gttcttcgca
    14881 aagcgtctgg ccgtgaggct gaggttgcgg aactccttgt ccgaccagag ggcgccgccg
    14941 atctcggctt cgaccatgga aatgcggtcg acggccgact ccccggctgc ggtcctgccg
    15001 ctgccgggct tcgcgggact ttcgtcctga agaatgtagg gctgccagtc gtcgatggac
    15061 gcacggggcg tctcgacgcg gattgcgatg ccttcggcgg gagcccgcat gggtgcggat
    15121 ccgaggcctg cgtagccgtg cctgagcgag aggccgtcct tgtcgcgggt gaagcgaagc
    15181 gccacggcgg cgcgtccggg gctcgatacg tcgacgtgca tggccttttc gggaagcgtg
    15241 acgctgaagg tcgtgggcca cttctcgccc gccgcctttt catagggctc gggcagcgta
    15301 ctcgcgatgc cgtcgaggtc cgtcctgccg gtgacggcga agcggctctt cgtgagcccg
    15361 atttcggcgt cgacatgaac gggagacgtg cccgtgagct ttttgaagaa gagctcggcg
    15421 acatcaatgc cgaggaagtg ctcgagttcg gctgtcgtcg gctcggcatc gatgccgagc
    15481 gtcatgacgc cgtccttcgt ttcggcggtg accttgacgg gcttgccgcc gggactcgtg
    15541 cccgtgaggg gcgtgggcgt gacgacgctc ttttccgtga tgtgcaggct cccattgacg
    15601 cccgagaccg tcggcaggtt gaggccgtag tagaaggtgg cgttgcggat gtccgcatcg
    15661 atgttgacgc gcaggtttct gacgatgtcg ccggaaaggg gaatatgaag gttcatgcgc
    15721 acgtcggcgg cgcccgaacc tttgctttcg gcgaacgcgg aggagaggat gtcgttgagc
    15781 atcctgccct gattgagata gcggagcacg cggccgaggt ctccctgggc gcggcccgtg
    15841 atctccagat cggcgtccgt gtaggacttc atgctgacga cgacgtcgct tgccttgagg
    15901 tcgtgcgagg ttgcgctccg gccgcggatt tcgaagccgt ttccgatgaa ggcgaggtgc
    15961 gcgtcgatgt tgttgagaac gggccaactc gaggcggtct cccaactgcc gtcggccttc
    16021 ctgacgtgcg aggggaggaa gtcgagcctg ccgcccgtca tgtcggcctc gatcgcaaag
    16081 tgtccgtcgt tcttgtgctt gccg
//
LOCUS       DBMDTI010000019        15802 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1182_length_15802_cov_2.631552, whole genome shotgun sequence.
ACCESSION   DBMDTI010000019 DBMDTI010000000
VERSION     DBMDTI010000019.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 15802)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15802)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15802
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1182_length_15802_cov_2.631552"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            157..1131
                     /locus_tag="ACZPB6_02090"
     CDS             157..1131
                     /locus_tag="ACZPB6_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1183302.1"
                     /translation="MILEAFLLSLNCVVILLILGGAGYVTAARGWYDKSSRVLLARLV
                     TFLSLPAYLFSSVMQTLDHDGLLTLVGSMATPFISIWTCFLLSRIIARVCHVEGVHSG
                     VFSSAFTATNNMFIGLPVSLALFGTEAMVPTLLYFFANTTFFWTVGNYMEAADGRLAT
                     MQKPQKVFSAQTFKRIFTPPLLGFLTAIVFILLDFTPPKAIMDAAHYMGGITTPLALV
                     FVGCMLYNIGIRNIRVTKDMLWVYIGRFVICPLVCLVLTYVIPIPPLFAKVYVIQAAL
                     PCITQIAVLAEFHHADVKFATTAVASTTLFSLAVIPAWMILVTMLIPS"
     gene            complement(1151..1858)
                     /locus_tag="ACZPB6_02095"
     CDS             complement(1151..1858)
                     /locus_tag="ACZPB6_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183303.1"
                     /translation="MSRFTVILPEGTVILKNEETGQILRLSAESGPLVLGEIEMSMTL
                     GPRPLEVAGAAHAPAVAAAQAPACELPARPADPLADLPDEAFVPRPETPGTAPLPHPI
                     PGTEGVNLINPYEALGKKALRSLPPGFGPNTGEPFMSPEEDWAPGTAPVLRGIRVLDA
                     PLEALTASDFEQILDAMALRRNNIEGEIEFMEEIEADEEDIRDLERDIEQLDRDLADV
                     VARKEEFDALSKKQAAE"
     gene            1987..2187
                     /locus_tag="ACZPB6_02100"
     CDS             1987..2187
                     /locus_tag="ACZPB6_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183304.1"
                     /translation="MYTFFSIVFGLIWAVASFLLLFKVWDVLGPIVLGISKNHIVQAA
                     ALVITYIVIFGVPAWCWVKLFG"
     gene            2460..3035
                     /locus_tag="ACZPB6_02105"
     CDS             2460..3035
                     /locus_tag="ACZPB6_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR1183305.1"
                     /translation="MTFTKFAVAAAAALLCAGSVIAAPVEGTDYVVLEKPIPNASGTF
                     IKVYSYDCPFCYKYDKSVVPAVKAKLDKEMKFLDYHLHTKGKYGKEGSEVLAVMWVAD
                     EAAGRDPYAADAFRATALEAVGMSKADFDAKKNDPKVQALVKAWADSTYDVAKIQGVP
                     AFVVNGKYLLKTASIRSIDGLVENIRELSKK"
     gene            3049..3717
                     /gene="dsbI"
                     /locus_tag="ACZPB6_02110"
     CDS             3049..3717
                     /gene="dsbI"
                     /locus_tag="ACZPB6_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HIR1183306.1"
                     /translation="MKGFCESFRKSPMTAFADWQETREPWILFGVTSIILVLLAHYLF
                     QGWLYMLPCEQCVYIRYGNIVMAIGAFLITINPKQALLKIAGIGVFLYGLIYTMICSW
                     KLMGIHDAVHSDDPMAMFGMQGCSTEPTYHFGLPLDKWAPDWFKQTGDCGYDAPVVPD
                     GTQLGDFQRWLIEVYQASDGWYLFPQWKFMDMAECCMLACVCYAALLLVLLAAWGMRA
                     MKKA"
     gene            complement(3891..5093)
                     /locus_tag="ACZPB6_02115"
     CDS             complement(3891..5093)
                     /locus_tag="ACZPB6_02115"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR1183307.1"
                     /translation="MAAFSIIGREGSVPVHVWAKTLDDKSREQLLNTASLPFVFHHVA
                     AMPDVHAGLGATIGSVIATEGAVIPSAVGVDIGCGMRLARLNFRRGDLDDRAVRAVYE
                     AILRHVPVGMTERRPDAVRHDRTDPFEEGMKSLLDRHPELLERMVSMTWRSQLGSLGA
                     GNHFIELTSDADGVVRVMLHSGSRGVGNVMASRFIGIAREKALGGKVRLPDPALAWFD
                     EGTDDFNDYMDAVNWAQAYAKINREIMMEDVMAAVREVMPGAEISSDEPVVDCHHNYV
                     ARETHYGRDVWVTRKGSVHAGRGELGIIPGSMGAASYLVEGLGSEESFMSSAHGAGRT
                     MSRTQAKKVFTVADLKRETAGVVCRHDPGVLDEIPSAYKSIDEVMAQQADLVRPVTVF
                     KQFLCVKG"
     gene            complement(5268..6554)
                     /gene="ccmI"
                     /locus_tag="ACZPB6_02120"
     CDS             complement(5268..6554)
                     /gene="ccmI"
                     /locus_tag="ACZPB6_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HIR1183308.1"
                     /translation="MLTTFIIICVVFCLIAMAAVALPLWFGGRKAADAEDRHETILGI
                     LRQQAADLEKEREAGRIDLDEYEESRLELERRVLEETARDTDAEVKDQSKVARIIAVV
                     LAVVIPASAVFGYLALGRYNAMDPAFLEAVQNQQRQTQGHSQSDMMAAIERLQNRLKE
                     TPDDAQTWYMLARTLSNVGRFAEALDAFREVDRLVPNNADIIADMADMTAAANNKVIT
                     DEARALLQRALKIDPGQWKALALLAIDAWDHEKYAEAAAYWEKLLVVLPEDFGDAQQI
                     RNNIDEARRLAGMDPAARQAEAEAFKGESVPTVQAAEPSFLSGRVTLDKAHADKVKPE
                     DTVFIYARPTTGSKMPVAFMRITAKELPFEFKLTSDMTMAMGATTLADVKDVIVGARI
                     SRTGNFMPQAGDLEGETSAPVQTGTEGIDLVIGTVR"
     gene            complement(6558..7043)
                     /locus_tag="ACZPB6_02125"
     CDS             complement(6558..7043)
                     /locus_tag="ACZPB6_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR1183309.1"
                     /translation="MKSVKLMGAALAACALMAGTAFAAPREAAPTAFDPIAHQRVVEV
                     SEQLRCLVCQNQSIADSNAELAVDLRNQVIEQVKAGKTNKEIVDYMVERYGDFVLYKP
                     PFKANTVILWLGPVALFLVGLAAFYLNLRRRKSAVAKTVRPLTAEEKARADKLLSGDK
                     E"
     gene            complement(7045..7584)
                     /locus_tag="ACZPB6_02130"
     CDS             complement(7045..7584)
                     /locus_tag="ACZPB6_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HIR1183310.1"
                     /translation="MSKSLKYLIPFGIFIVLAGFLFKGLYMDPRQLPSVLINKPMPQW
                     SLPTLFDTTKTLSTEEMKGKVWMLNVWATWCIPCKQEHPYMVKLKKDGLKTPIVGFVY
                     KDNPGAALNMLRQQGNPYDMVVFEVKNRVGLDLGVTGVPETFVIDKKGVIRAKVSYPL
                     DPQIWNEQLKPLIKQLEAE"
     gene            complement(7588..9546)
                     /locus_tag="ACZPB6_02135"
     CDS             complement(7588..9546)
                     /locus_tag="ACZPB6_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR1183311.1"
                     /translation="MIPEIGHFALILCLALSIVQGVLPLVGAAKGNRALMAVARPAAA
                     ANAFFGTVAIGCLAYSFYLSDFTVLNVANNSNSLLPWYYKVAATWGSHEGSILFWTVT
                     LGWWGAAVAFCARRLPAEMVARVTGVLGLVGMGFLLFMLLTSNPFLRLFPAPAEGADL
                     NPLLQDPGMVFHPPLLYLGYVGFAVPFAFAIAALISGRLDAAWARWMRPWTTAAWVLL
                     TLGIALGSYWAYYELGWGGWWFWDPVENSSFMPWLTGTALIHSLAVTEKRGCFRIWTV
                     LLAILTFSLSLLGTFLVRSGVLTSVHAFATDPERGLFILAFLIIVIGLSFLLFAWRAP
                     TVGLGGNFSLISRESMLLVNNVLLVVAMGAVLLGTLYPLFLDALNAGKISVGPPYFDA
                     VFGPLMLPCVFLMGVGPLARWKDADPKALARELAWCLVAAIVAGAAIPLLMGEFGHWV
                     FLGCTSAMFVFFAVIQTFRHQIRNQPGNVFARLMRQPRAFWGMQLAHIGVAVFIIGVA
                     LVKGYQSERDVRMYEGETVTVAGYTFTFNGVETVRGPNYTADRGDFTLSVNGRELQHL
                     YPEKRKYYSSNSMPMTESAIRHSITGDVYVSLGTPTNDGGWVVRAYYKPYVTWIWWGC
                     IIMAAAGLWAASDRRYRRRKQKNEADGE"
     gene            complement(9694..10167)
                     /gene="ccmE"
                     /locus_tag="ACZPB6_02140"
     CDS             complement(9694..10167)
                     /gene="ccmE"
                     /locus_tag="ACZPB6_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR1183312.1"
                     /translation="MDSKTKKLALIGAAVVAVGVGVTLALQAFNENLVFFFSPTQVAA
                     KEAPTGRTFRLGGVVEPGSVKRQADGVTVTFGITDTAKIVPVQYKGILPDLFAEGQGV
                     VAQGKLDEQGFFCASEVLAKHDENYMPPEAAKALEDAHKAGVAKKQAEQAQAAKS"
     gene            complement(10171..10359)
                     /gene="ccmD"
                     /locus_tag="ACZPB6_02145"
     CDS             complement(10171..10359)
                     /gene="ccmD"
                     /locus_tag="ACZPB6_02145"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HIR1183313.1"
                     /translation="MNNWNSLSDFIYMNGHGYFVWCAYGALLVAVIYEIFSLRARRAK
                     ICARLAREARAAKSTLEM"
     gene            complement(10356..11081)
                     /locus_tag="ACZPB6_02150"
     CDS             complement(10356..11081)
                     /locus_tag="ACZPB6_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HIR1183314.1"
                     /translation="MSLSDPQNFYRLAGKVIPWILGLALVLFVVGFYLGFFVCPVDAR
                     QGNSYRIIFIHVSAAWLSMLLYVLMAVFSAIGLWRNNRICFMLAQAMAPTGALMAFIA
                     LFSGAFWGRPTWGTYWVWDARLTSELILFFLYLGFIALHSAIDDKRRADRAASVISIV
                     GVINVPIIYFSVQWWNTLHQGASITSRGSSIAPIMLYTLLIMAVAGMCYGAGMVLART
                     RSIILERERRENWTKEAALEGKL"
     gene            complement(11140..11808)
                     /gene="ccmB"
                     /locus_tag="ACZPB6_02155"
     CDS             complement(11140..11808)
                     /gene="ccmB"
                     /locus_tag="ACZPB6_02155"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR1183315.1"
                     /translation="MFKLFRIILRRDLMLAFRRKSDLAQVIFFFAVVVTLVPLGVGAE
                     TNILRSIAPGVVWVAALLAALLSLPRMFEADHADGTLEQMLVTAEPLPVVVVAKVFAH
                     WLVTGVPMTIFAAVFGILFDLPLDVTLVMVASLFLGTPVLSLVGAVGAALTLGLRGGG
                     VLTSLLVLPLYIPVLIFGAGASQAVAVSMSPAAHFMIVGGLTLLSLALAPMAVSAALR
                     IAES"
     gene            complement(11823..12530)
                     /gene="ccmA"
                     /locus_tag="ACZPB6_02160"
     CDS             complement(11823..12530)
                     /gene="ccmA"
                     /locus_tag="ACZPB6_02160"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HIR1183316.1"
                     /translation="MSEAILEVCGLTCERGERTLFRNLSFAVKPGTLVRIAGSNGAGK
                     TTLLRLMTGLMRPVEGEVRWRGVPVEKAAQDFWRELCYIGHRNGVKDDLSVLENVMIN
                     ANVAAVPVTENAALDALDAVGLADFVDVPAGQLSQGQRRRVALARLWLSRSVTLWVLD
                     EPFTALDVRGVARLADLVGEHVRSGGIVMLVTHQEVPVDGVEYLVIEPERWSPRRRTA
                     VEAALDNESALEADNAR"
     gene            12986..13399
                     /locus_tag="ACZPB6_02165"
     CDS             12986..13399
                     /locus_tag="ACZPB6_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183317.1"
                     /translation="MHMKRTLIIGTALAALLLSGCEALQEERQNFLDREEKITNVVGN
                     VWRIEAVWPGHVEGNRLADHLHERARMHCDRQHMAMLPVNGSSTNGKKDGSRPASAWL
                     EFRCQPGLDYRPEYKGLTGTFEIDDLTTLENTQGK"
     gene            13576..15369
                     /locus_tag="ACZPB6_02170"
     CDS             13576..15369
                     /locus_tag="ACZPB6_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1183318.1"
                     /translation="MSVSPSSSARLTGAAAAILLASAPVFVHAAPGAVPPVVVAVQAS
                     GSGAIERSFISASVDHLTYALPDTPLVFRYLPLGEIQKLAESRRVDFVLAPPDGYVVL
                     ERFFGARSLVSQTIRESSSADSVLGIVVAVNAAEAPDASASVLNGASILVPELDGMPA
                     RMLRRELMHAGVERAVPEALRTKTDDPEGAILEAVASGRADAGILPACYVERLSATDP
                     KRLSGIRLVTTDRASQTRCRSFSVFYPGWTLASFPHTPPELNALVSGTLLGMPTDADG
                     CDWRPGARYDAVHELLEALGDAAYLRQSQFSWEAFVRNYGVWLAFLAAVLLGIVVHSV
                     RAEYLIRRRTTELMRAVEEKRRVEEEAKRYNERLVAMERAGLVGELSSMIAHELKQPL
                     AVIRNYARGLSRTMAHGGSTDPEVFRTVLAKIDSQSTKASDIIDHVRSFARQPAQPVP
                     TSLSDLAEKAIAQFCATRGLEVSRRIEPGVTILADALEMELLISNLLKNAADATAGTD
                     DAVIRASLSAEDGTAMLTISDNGPRLTDEQFARLTIPLNTSKPQGLGLGLVIVRRIAE
                     SARGRVLFERLPERGLAITVSLPVCEQNNRT"
     gene            15371..15709
                     /locus_tag="ACZPB6_02175"
     CDS             15371..15709
                     /locus_tag="ACZPB6_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1183319.1"
                     /translation="MPANSLSLIRIVDDDAEMRESLEFLLSTEGWKSRSYASAEAFLE
                     TDDVMVPGCLILDIRMPGLSGLQLQELLKKKDYSLPILFITAHGDITMAVEAVKNGAF
                     DFLPKPLDGQ"
BASE COUNT         2885 a         5098 c         5037 g         2782 t
ORIGIN      
        1 aacgacggaa tcagcaagtc cattattgaa gatgcggcag aacgcaatat tcgcctgatc
       61 gttcttgaag atcttacgga catccgcgag cgaatcaagg ccgggcggcg ctaccattga
      121 aggatcccgc aagtcttcac ccgccggccg cgtctcatga ttctcgaagc ctttcttctg
      181 tccctcaact gcgtcgtcat cctgctgatt ctcggcggcg caggctacgt gacggccgca
      241 cggggctggt acgacaagag ctcccgcgtc ctgcttgcgc gcctcgtgac ctttctctcc
      301 ctgcccgcct acctcttctc gagcgtcatg cagacgcttg accacgacgg actcctgacg
      361 cttgtgggtt cgatggcgac gcccttcatc tcgatctgga cctgcttcct gctgagccgc
      421 atcatcgcgc gcgtctgcca tgtcgagggg gtgcattcgg gcgtcttttc gagcgccttc
      481 acggcaacga acaacatgtt catcggcctg cccgtctccc tcgcgctctt cggcaccgag
      541 gcgatggtgc ccacgctcct ctacttcttt gcgaacacca cgttcttctg gacggtcggc
      601 aactacatgg aggcggccga cgggaggctt gccacgatgc agaagcccca gaaggtgttc
      661 tcggcgcaga ccttcaaacg tatcttcacg ccgccgctcc tcggcttcct cacggccatc
      721 gtgttcatcc tgctcgactt cacgcctccg aaggcgatca tggacgccgc gcactacatg
      781 ggcggcatca cgacgccgct cgcgctcgtc ttcgtgggct gcatgctcta caacatcggc
      841 atccgcaaca tccgcgtcac gaaggacatg ctctgggtct acatcgggcg cttcgtgatc
      901 tgcccgctcg tgtgccttgt gctcacgtac gtcattccca ttccgccgct ctttgcgaag
      961 gtctacgtca ttcaggcggc gctgccctgc atcacgcaga tcgccgtgct cgccgaattc
     1021 caccacgccg atgtcaagtt cgcgacgacg gccgtcgcct cgacgacgct cttctcgctc
     1081 gccgtgatcc ctgcgtggat gatcctcgtg acgatgctca tcccgagctg atcacggcgg
     1141 accttccgac ctattccgcg gcctgcttct tcgaaagcgc gtcgaactct tccttcctcg
     1201 cgaccacgtc ggcaaggtca cggtcgagct gctcgatgtc acgctcgaga tcgcggatgt
     1261 cctcttcgtc cgcctcgatc tcctccatga actcgatttc gccctcgatg ttgttgcgcc
     1321 tgagcgccat cgcgtcaaga atctgctcga agtccgaggc cgtcagcgcc tcgagcggcg
     1381 cgtcgagcac gcggatgccg cgaagaacgg gggccgtgcc gggcgcccag tcctcctcgg
     1441 gcgacatgaa gggctcgccc gtgttgggac cgaatcccgg cggcaggctc ctcaaggcct
     1501 tctttccgag cgcctcatag ggattgatga gattcacgcc ctcggtcccc ggaatcgggt
     1561 gcggaagcgg cgcggtgcca ggcgtctcgg gtctgggcac gaaggcctcg tcgggcaggt
     1621 ccgccagggg atcggcgggc cgggcgggga gttcacaggc gggggcctgc gctgcagcaa
     1681 cagcgggagc gtgcgccgca cccgcaacct cgaggggcct aggcccgagc gtcatcgaca
     1741 tctcgatttc gccgagcacg agcggacccg actcggcgga aagcctcaga atctgccccg
     1801 tctcctcgtt cttgagaatg acggtgcctt cgggaagaat gacagtaaag cgggacattc
     1861 ggatcggacc tcgtgacggg aaagaacgct gtaatctaat attaagcgca aagacctact
     1921 attatcgggc aacagagtca ttccaacccg tgcctgccgc acgatgacca acaaaggatt
     1981 ttttcgatgt acaccttctt ctccatcgtt ttcggcctca tctgggctgt cgcctcgttc
     2041 ctccttctct tcaaggtctg ggatgttctc ggcccgatcg tgctcggcat ctcgaagaac
     2101 cacatcgtgc aggccgccgc gctcgtcatc acctacatcg tgatcttcgg cgtccccgcc
     2161 tggtgctggg tgaagctctt cggctgaccc gcagcccgta catgacgccc tcgccatgaa
     2221 tccgcgcccg cccatcctcg accgatgcgg cgggcgccgt cgtcatgggc catgcgtcga
     2281 gcaggccgaa cccgtttttc agcatttgag aaaagcgact tgtcaaattc tgaaagttcc
     2341 cgtgggaagt caacaatttt caaaatttcc gtcgacgcct aaacttcctc acacagttga
     2401 ggcgggcccg tatcctgcgg ccgccgacca tgcttgacat tccgaccgag gagaacacca
     2461 tgaccttcac gaaattcgcc gtcgccgccg cagccgccct gctctgcgca ggctccgtca
     2521 tcgccgcccc cgtcgagggc accgactacg tcgtccttga aaagccgatc ccgaacgcct
     2581 cgggcacctt catcaaggtc tactcctacg actgcccgtt ctgctacaaa tacgacaagt
     2641 ccgtcgttcc ggccgtcaag gccaagctcg acaaggaaat gaagttcctc gactaccatc
     2701 tccataccaa gggcaagtac ggcaaggaag gctccgaggt cctcgccgtg atgtgggtcg
     2761 ccgacgaagc cgccggccgc gatccgtatg ctgccgacgc cttccgcgca accgccctcg
     2821 aggctgtcgg catgtccaag gccgatttcg acgccaaaaa gaatgatccg aaggttcagg
     2881 cgctcgtgaa ggcctgggcc gacagcacct acgacgtcgc caagatccag ggcgtgccgg
     2941 ccttcgtggt caacggcaag tacctcctga agacagcctc catccgctcc atcgacggtc
     3001 tcgtggaaaa catccgcgaa ctctccaaga agtaagaggt cctgacaaat gaagggattc
     3061 tgcgaaagct tccgaaagag tccgatgacg gccttcgccg actggcagga gacgcgcgag
     3121 ccgtggatcc tcttcggcgt gacctcgatc atcctggtgc ttctggccca ctacctcttc
     3181 cagggctggc tctacatgct gccctgcgag cagtgcgtct acatccgcta cggcaacatc
     3241 gtcatggcga tcggcgcctt cctcatcacg atcaatccga agcaggctct gctgaagatc
     3301 gccggcatcg gcgtcttcct ctacggcctc atctacacga tgatctgctc ctggaagctc
     3361 atgggcatcc atgacgccgt ccactccgac gacccgatgg ccatgttcgg catgcagggc
     3421 tgctcgacgg agccgactta tcacttcggc cttccgctcg acaagtgggc tcccgactgg
     3481 ttcaagcaga cgggcgactg cggctacgac gcccccgtcg tgcccgacgg cacccagctc
     3541 ggcgacttcc agcgctggct catcgaggtc tatcaggcga gcgacggctg gtacctcttc
     3601 ccgcagtgga agttcatgga catggctgaa tgctgcatgc tcgcctgcgt ctgctacgcg
     3661 gcgcttctcc tggttcttct cgccgcctgg ggcatgcgcg ccatgaagaa ggcctgatcc
     3721 tccacagtgt cttcacccgg ccgctccgag caaagccggg ggcggttccg gttcaaccgc
     3781 caaccggctg acgcagcgtc tctggtccga cgccacgccg gccatcctcc caagcaatgg
     3841 gcgcccgacg gcatcaaccg tcaggcgccc attgtccatt tggcgtcagg tcaacccttc
     3901 acgcagagga actgcttgaa gacggtcacg ggtctcacga ggtcggcctg ctgcgccatc
     3961 acctcgtcga tggacttgta ggccgacgga atctcgtcga gcacgccggg atcgtgcctg
     4021 cagacgacgc ccgccgtctc gcgcttcagg tcggcaaccg tgaagacctt tttggcctgc
     4081 gtgcggctca tcgtgcggcc cgcgccgtgc gcggacgaca tgaaggactc ctcggagccg
     4141 agtccctcga ccagatacga ggcggcgccc atgctccccg ggatgatgcc gagctcgccc
     4201 ctgcccgcat gaacggagcc cttgcgcgtc acccatacgt cgcgcccgta atgcgtctcg
     4261 cgagccacgt agttgtgatg gcagtcgacg acgggctcgt cggaggagat ctccgccccc
     4321 ggcatcacct cgcgcaccgc ggccatgacg tcctccatca tgatctcgcg gttgatcttc
     4381 gcataggcct gcgcccagtt gaccgcatcc atgtagtcgt tgaagtcatc ggtgccctca
     4441 tcgaaccaag caagcgcggg atcgggcagc cggaccttcc ctccaagtgc cttctcgcgg
     4501 gcgatgccga tgaagcgcga ggccatgacg ttgcccacgc cgcgcgaacc cgaatggagc
     4561 atcacgcgca cgacgccgtc tgcatcagag gtgagttcga tgaagtggtt gcccgctccc
     4621 aggcttccga gctggctccg ccaggtcatg gacaccatgc gctcgaggag ttcgggatga
     4681 cggtcgagaa ggctcttcat gccttcctcg aaggggtccg tgcggtcgtg acgcacggcg
     4741 tcgggcctcc tctcggtcat gccgacgggc acgtgccgca gaatcgcctc gtagacagcg
     4801 cggaccgcgc ggtcgtcaag gtcaccgcgc ctgaagttga gtcgtgcaag gcgcatgccg
     4861 cagccgatgt cgacgccgac ggcgctcggg atgacggcgc cttcggtcgc gatcacgctg
     4921 cctatggtgg cgcccagacc tgcatgaacg tcgggcatcg ccgcgacgtg atgaaagacg
     4981 aacggcaacg aggccgtgtt gagaagctgc tcacgcgact tgtcgtcgag cgtcttcgcc
     5041 cagacgtgaa cagggacgct tccttcgcgt ccgatgatgg aaaaggccgc catggcggta
     5101 cctccacatt agttcttttt agtttcagtt gatatatttc acctttttcg caacaagggt
     5161 tcaattatat gaagaagtgc tcgcccgcac acgcgggaaa acacctaaaa cgcaaaggcc
     5221 gccgtcgttc agtcgaacgc ggcggccttt tctgaggctg tcgaatgtca gcggacggtg
     5281 ccgatgacga ggtcgatgcc ttcggtgccc gtctggacgg gagcggaagt ctcgccctcg
     5341 aggtcgcccg cctgcggcat gaagttgccg gtgcgggaga ttcgggctcc gacgatcacg
     5401 tccttgacgt cggcgagcgt cgtggcgccc atggccatcg tcatgtcgct cgtgagcttg
     5461 aactcaaagg ggagctcctt ggcggtgatg cgcatgaagg cgaccggcat tttcgagccc
     5521 gtggtcgggc gtgcatagat gaagacagtg tcctcgggct tgaccttgtc ggcgtgggcc
     5581 ttgtcgagcg tcacgcggcc ggagagaaac gagggctcgg cggcctggac ggttgggacg
     5641 ctctcgccct tgaaggcttc ggcctcagcc tgacgggctg cgggatccat gccggcgagg
     5701 cggcgggcct cgtcgatgtt gttgcggatc tgctgggcgt cgccgaagtc ctcgggaagc
     5761 acgacgagga gcttttccca gtaggcggcg gcttcggcgt acttctcatg atcccaggcg
     5821 tcgatggcaa ggagcgcgag cgccttccac tggcccggat cgatcttgag ggcgcgttgc
     5881 agaagcgcac gggcctcgtc cgtgatcacc ttgttgttcg cggcggccgt catgtcggcc
     5941 atgtcggcga tgatgtcggc gttgttcggc accagacggt cgacctcgcg gaaggcgtcg
     6001 agcgcctcgg cgaagcgacc cacgttggag agcgtgcgcg cgagcatgta ccaggtctgg
     6061 gcgtcgtcgg gcgtctcctt caggcggttc tgaaggcgct cgatggcggc catcatgtcg
     6121 gactggctgt ggccctgcgt ctggcgctgc tggttctgga cggcctcgag gaaggcgggg
     6181 tccatggcgt tgtagcggcc tagcgcaaga taaccgaaga cggcggaggc gggaatcacg
     6241 acggcgagca cgacggcgat gattcgggcg accttggact ggtccttgac ttcggcgtcg
     6301 gtgtcgcggg cggtttcctc aagcacgcgg cgctcgagtt cgaggcggga ctcctcgtac
     6361 tcgtcgaggt cgatgcggcc ggcctcacgc tccttttcaa ggtccgcggc ctgctgacgc
     6421 aggatgccga gaatcgtttc gtggcggtct tcggcgtcag cggccttgcg gccgccgaac
     6481 cagagcggca gcgccacggc ggccatcgcg atcaggcaga agacgacgca gatgatgatg
     6541 aaggttgtca gcatcggtta ctccttgtcg cccgagagca gcttgtccgc gcgggccttt
     6601 tcctcggcgg tgagcggacg gacggtcttg gcgacggccg acttgcggcg gcggaggttg
     6661 aggtagaacg cggcgaggcc cacgaggaag agtgcgacgg ggccgagcca gaggatgacc
     6721 gtgttggcct tgaagggcgg cttgtagagc acgaagtcgc cgtagcgttc gaccatgtag
     6781 tccacgatct ccttgttggt cttgccggcc ttgacctgct cgatcacctg gttgcgcagg
     6841 tcgacggcga gctcggcgtt ggagtcggcg atggactggt tctggcagac gaggcagcgg
     6901 agctgctcgg aaacctcgac gacgcgctgg tgggcgatcg ggtcaaaggc ggtcggagcg
     6961 gcttcgcgcg gagctgcgaa ggccgtgccg gccatcagtg cgcaggccgc gagggcggcg
     7021 cccataagct tgacggactt catgttattc ggcctccagc tgcttgatga ggggcttgag
     7081 ctgctcgttc cagatctgcg gatcgagcgg gtagctcacc ttggcccgga tcacgccctt
     7141 cttgtcgatg acgaaggtct cgggaacgcc cgtgacgccg aggtcaagac cgacccggtt
     7201 cttcacctcg aagacgacca tgtcgtaagg attgccctgc tggcgcagca tgttgagggc
     7261 ggcgccagga ttgtccttgt agacgaagcc aacgatcggg gtcttgaggc cgtccttctt
     7321 gagcttgacc atgtaggggt gctcctgctt gcagggaatg caccacgtgg cccagacgtt
     7381 gagcatccag accttgccct tcatctcctc ggtggagagc gtcttcgtgg tgtcgaagag
     7441 cgtcgggagc gaccactgcg gcatcggctt gttgatgagc acgctgggca actgtcgcgg
     7501 atccatgtag agccccttga agaggaagcc cgcgaggaca atgaagattc cgaaaggaat
     7561 cagatacttg agagacttgc tcatgactta ttctccgtca gcttcgttct tctgcttgcg
     7621 gcggcggtag cgtcggtcgg acgcggccca aaggccagcg gcggccatga tgatgcagcc
     7681 ccaccagatc caggtgacgt aaggcttgta gtaggcgcgc acgacccagc cgccgtcatt
     7741 ggtcggcgtg ccgaggctca cgtacacgtc gcccgtgatc gagtggcgga tggcggactc
     7801 ggtcatcggc atcgagtttg agctgtagta cttgcgcttt tcaggataga ggtgctggag
     7861 ctccctgccg ttcacggaga gcgtgaaatc gccgcggtcg gccgtgtagt tcgggccgcg
     7921 gacggtttcg acgccgttga acgtgaaggt gtagccggcg accgtcacgg tctcgccttc
     7981 gtacatgcgc acgtcgcgtt cggactggta gcccttgacg agcgccacac cgatgatgaa
     8041 gacggccacg ccgatgtggg cgagctgcat gccccagaag gcgcgcggct ggcgcatcag
     8101 gcgggcgaag acattgcccg gctggttgcg gatctgatga cggaacgtct ggatcacggc
     8161 aaagaagacg aacatcgcgg acgtgcagcc gaggaagacc cagtggccga actcgcccat
     8221 gaggagcggg atggcggcgc cggccacgat ggcggcgacg aggcaccagg cgagctcgcg
     8281 ggcgagcgcc ttcggatcgg cgtccttcca gcgggcgagc ggacccacgc ccatcaggaa
     8341 cacgcacggc agcatgagcg gaccgaagac ggcgtcaaag tacggggggc cgaccgagat
     8401 cttgcctgcg ttgagcgcgt cgaggaagag cggatagagc gtgccgagaa gcacggcacc
     8461 catggccacg acgagcagca cgttgttgac gaggagcatc gactcgcggg agatgagcga
     8521 gaagttgccg cccagaccca ccgtcggggc gcgccatgca aagagcagga acgagaggcc
     8581 gatgacgatg atgaggaagg ccagaatgaa gagaccgcgc tcggggtcgg tcgcgaaggc
     8641 gtgcaccgag gtgagcacgc cggagcgcac gaggaaggtg ccgaggagcg agagcgagaa
     8701 cgtcaggatc gcgaggagca ccgtccagat gcggaagcaa ccgcgctttt cggtgacggc
     8761 gagcgagtgg atgagcgcgg tgcccgtgag ccacggcatg aacgaggagt tctcgaccgg
     8821 gtcccagaac caccagccgc cccagccgag ctcgtagtag gcccagtagg agccgagcgc
     8881 gatgccgagc gtgaggagta cccaggcggc cgtcgtccac ggacgcatcc agcgggccca
     8941 ggcggcgtca agacgaccgg agatgagcgc ggcgatcgcg aaggcgaacg gcacggcgaa
     9001 gcccacgtag cccagataga ggagcggcgg atggaaaacc atgcccgggt cctgcagcag
     9061 cggattgagg tccgcgccct cggcgggcgc agggaagagg cgcaggaacg gattggaggt
     9121 gagcagcatg aagaggagga agcccatgcc gacgaggccg agcacgcccg tcacgcgggc
     9181 gaccatctcg gcgggcaggc ggcgggcgca gaaggcgacc gcggcgcccc accatccgag
     9241 cgtcaccgtc cagaagagga tcgagccttc gtgggagccc caggtcgcgg ccaccttgta
     9301 gtaccagggc agaagcgagt tggagttgtt cgccacgttg aggacggtga agtccgacag
     9361 gtagaacgag taggcaaggc agccaatggc caccgtgccg aagaacgcgt tggcggcagc
     9421 ggcgggacgg gcgaccgcca tcagcgcgcg gttgcccttg gcagcgccga caagcgggag
     9481 cacgccctgg acgatgctca acgcaaggca caggatcagt gcgaagtgac cgatttcagg
     9541 aatcacttgt tttctccggt ttgtgtatgg ggacggatgc ttccgtcttc tctctttatg
     9601 caagcaaggg catggccgct ttgcctggcc ctcgggtcag gcgggcggtc acacccggct
     9661 caataaaaac tcaattgact ggaagggcag gcatcaggac ttcgcggcct gggcctgctc
     9721 ggcctgcttc ttggctacgc ccgccttgtg ggcgtcttcg agagccttgg cagcctcggg
     9781 cggcatgtag ttctcgtcgt gcttggcgag cacttcggag gcgcagaaga agccctgctc
     9841 gtcgagcttg ccctgggcca cgacgccctg gccttcggcg aagaggtcgg ggaggatgcc
     9901 cttgtactgg acgggaacga tcttggcggt gtcggtgata ccgaaggtga cggtcacgcc
     9961 gtcggcctgg cgcttgacgc tgccgggctc gaccacgccg ccgagcctga acgtgcggcc
    10021 cgtcggggct tccttcgcgg ccacctgcgt gggcgagaag aagaagacga ggttttcatt
    10081 gaatgcctgc agggcgagcg tcacgccgac gccgacggcc acgacggccg cgccgatgag
    10141 ggcgagtttc ttggtcttcg agtccatggt ttacatctcc agcgtcgatt tggctgcacg
    10201 ggcctcgcgg gcgaggcgag cgcagatctt ggcgcggcgg gcgcgcaggg aaaagatttc
    10261 gtagatcacg gcgacgagca gcgcgccgta ggcgcaccaa acgaagtagc cgtggccgtt
    10321 catgtagatg aagtcggaaa gactgttcca gttgttcaca gcttgccctc caaggccgct
    10381 tccttggtcc agttctcgcg gcgttcgcgt tcgaggatga tggagcgggt gcgggcgagg
    10441 accatgccgg caccgtagca catgcccgcc acggccatga tgaggagcgt gtagagcatg
    10501 atgggcgcaa tggaggagcc cctcgacgtg atcgaggcgc cctgatggag cgtgttccac
    10561 cactgcacgg agaagtagat gatcggcacg ttgatcacgc ccacgatgct gatgacgctc
    10621 gcggcgcggt cggcgcggcg cttgtcgtca atggcggagt gcagcgcgat gaagccgaga
    10681 tagaggaaga agaggatgag ctccgacgtc aggcgagcgt cccacaccca gtacgtgccc
    10741 caggtcggac ggccccagaa ggcgccagag aagagcgcga tgaaggccat cagcgcgccc
    10801 gtgggggcca tcgcctgggc aagcatgaag cagatgcggt tgttgcgcca cagaccgatg
    10861 gcgctgaaga cagccatcag cacgtagagc agcatcgaga gccaggcggc cgagacgtgg
    10921 atgaagatga tgcggtagga gttgccctgg cgcgcatcga ccgggcacac gaagaagccc
    10981 aggtagaagc ccacgacgaa gagcacgagt gccagcccga gaatccacgg gatgaccttg
    11041 cctgcgaggc ggtagaagtt ctgagggtcg gataagctca tcttgatcag gtctccgaat
    11101 atattttcaa aagtctttcc agcgttcgct tcgcccgcat caactttcgg cgatgcgaag
    11161 ggccgcggac acggccatcg gggcgagcgc gaggctcagc agcgtcaggc cgcccacgat
    11221 catgaagtgc gcggcggggc tcatcgagac ggcgaccgcc tggctggcgc ccgcgccgaa
    11281 gatgaggacg ggaatgtaga gcggaagcac gagcaggctc gtgagcacgc cgcctccgcg
    11341 aaggccgagc gtgagtgcgg cgcccacggc tcccacgagg gagaggaccg gcgtgccgag
    11401 gaagaggctc gcgaccatca ctagggtcac gtcgagcggc aggtcgaaga ggatgccgaa
    11461 aaccgcggcg aagatcgtca tcggaacgcc cgtgacgagc cagtgcgcga agaccttggc
    11521 tacgaccacc acgggaagcg gctcggcggt gacgagcatc tgctcgagcg tgccgtcggc
    11581 atgatcggcc tcgaacatgc gcggcagcga gagaagcgcg gcgagaagcg cggcgaccca
    11641 cacgacgccg ggggcgatcg agcggaggat gttggtttcg gcgcccacgc cgagcggcac
    11701 gagcgtcacg acgaccgcaa agaagaagat cacctgcgca aggtcggact tgcggcggaa
    11761 ggcgagcatc aggtcgcggc ggaggatgat tcggaaaagc ttgaacatga ctgcggcgct
    11821 ccttaccttg cgttgtccgc ttcaagagcg ctttcgttgt cgagcgcagc ctcgaccgcc
    11881 gtgcggcggc gcggggacca gcgttcgggc tcgatgacga ggtattcgac gccgtcgacg
    11941 ggcacttcct gatgggtgac gagcatcacg atgccgcccg acctgacatg ctcgcccacg
    12001 aggtccgcaa ggcgggcgac gccgcgcacg tcgagcgcgg tgaagggctc gtcgagaacc
    12061 cagagcgtca cggagcggga aagccagagg cgggcgagcg cgacgcggcg gcgctggccc
    12121 tgagagagct ggcccgcggg cacgtcgacg aagtcggcca ggcccacggc gtcaagtgcg
    12181 tcaagcgcgg cgttctccgt gacgggcacg gccgccacgt tggcattgat catcacgttc
    12241 tcgagcaccg acaggtcgtc cttgacgccg ttgcggtgtc cgatgtagca gagctcgcgc
    12301 cagaagtcct gggcggcctt ctcgacggga acacccctcc agcgtacctc accctcgacg
    12361 ggacgcatga ggcccgtcat cagtcgcaga agcgtcgtct tgcccgcacc gttgctgccg
    12421 gcgatgcgca cgagcgtgcc gggcttcacg gcgaacgaga gattcctaaa aagcgtcctc
    12481 tccccgcgct cgcaggtaag gccgcagact tcaagaatgg cttctgacat gtgtgaaacg
    12541 gagggtcctg ttgacgttgt tgacgggcac ggttcgggcc cggatggtct tgtgtcagga
    12601 agtgtaggga ggccgtatta agcattgctt acaaaatgtc tcttcttcca accgtgctaa
    12661 ccctagtggc acagggcccg agaacccgaa aaactcacag agcagcaggc gttttcagca
    12721 aatcaccgcc ccttcggccc gtctttcctc atctccccgc accttatgaa acgaggacgc
    12781 gggcgcccgc atgcggccgc ggccttctca gaggccccga cgcctatcgg ccagattgag
    12841 aaagccttcc cttttcgtcc atttttctgt ttcttgctcc atgcaccgcg cctcgcgacg
    12901 cccgggcgga atcccgctaa aatgtcggaa ccatcgcccg cccgtatgcg gcgcggcctc
    12961 cgaccattcg acagactttc tacccatgca tatgaagcgc actctgatca tcggcaccgc
    13021 cctcgccgcc cttcttctct ccggttgcga agcccttcag gaggaacgcc agaacttcct
    13081 cgaccgcgag gagaagatca cgaacgtggt cggcaacgtc tggcgcatcg aggccgtctg
    13141 gcccggacac gtcgagggca accgcctcgc cgaccacctc catgaacgcg cccgcatgca
    13201 ctgcgaccgc cagcacatgg ccatgctgcc cgtcaacggt tcctcgacca acggcaagaa
    13261 ggacggcagc cgtcccgcct ccgcatggct cgaattccgc tgccagccgg gtctggacta
    13321 ccgccccgaa tacaagggcc tcaccggcac cttcgagatc gacgacctga cgacgctcga
    13381 aaacacgcag ggcaagtgag cccgagtgcc ccttccggct cccgcaccgc ccgcacgcct
    13441 tcgtgcgggc ttttttgcac ctccccgcca acgcccgccg ctcaggcgtt ttcgtacgga
    13501 agggagtttc catcagtgtt ttccccgccc gcaagggggt tgggactact taaataacgg
    13561 acatacaata agagaatgtc cgtctcgcca agctcatccg cccgactcac aggggcggcc
    13621 gccgccatcc ttcttgcgtc cgcaccggtc ttcgtccatg cggccccggg agccgtgccg
    13681 cccgtcgtgg tcgccgtgca ggcctcgggc tcgggtgcga tcgagcgaag cttcatcagt
    13741 gcgagcgtgg accatctcac ctatgcgctt ccggacacgc cgctcgtatt ccgctacctg
    13801 ccgctcggcg agatccagaa gctcgccgag tcgagaagag tcgacttcgt gctcgcgccg
    13861 cccgacggct acgtcgttct cgaacgcttc ttcggcgccc gctcgctcgt gagccagacg
    13921 atccgcgaat cctcctcggc cgactcggtc ctgggcatcg tcgtcgccgt gaacgccgcg
    13981 gaggctcccg acgcctccgc ctccgtcctc aacggcgcct ccattctcgt gccagaactt
    14041 gacggcatgc ccgcccgcat gctcaggcga gagctcatgc acgcaggcgt cgagcgcgcc
    14101 gtgcccgaag ccctgcgcac gaagacggac gatcccgaag gcgccattct tgaagccgtc
    14161 gcctcgggac gcgccgacgc gggcatcctg cccgcctgct acgtcgagcg gctctccgcg
    14221 accgatccca aaagactttc gggcatcagg ctcgtcacga ccgaccgtgc ctcgcagacg
    14281 aggtgcaggt ccttctccgt cttctatccc ggctggacgc tcgcctcctt tccgcacacg
    14341 ccgcccgaac tcaacgcgct cgtctcgggc acgctgctgg gcatgcccac ggacgccgac
    14401 ggctgcgact ggcggccggg cgcacgatac gacgccgtgc acgagcttct tgaggcgctc
    14461 ggcgacgcgg cctacctgcg ccagtcccag ttcagctggg aggccttcgt cagaaactac
    14521 ggcgtctggc tcgcatttct cgccgccgtg ctgctcggca tcgtcgtgca cagcgtgcgc
    14581 gccgaatacc tgatccgccg ccgcaccacg gagctgatgc gggccgtgga ggagaagcgg
    14641 cgcgtcgagg aggaagccaa acgctacaac gaaaggctcg tcgcgatgga gcgtgcgggc
    14701 ctcgtgggcg agctctcctc gatgatcgcg cacgagctca agcagccgct cgccgtcatc
    14761 cgcaactacg cccgcggcct ctcccgcacc atggcccacg gcggctccac cgaccccgag
    14821 gtcttcagga ccgtactcgc caagatcgac tcccagagca cgaaggcctc ggacatcatc
    14881 gaccacgtca gaagcttcgc aagacagccc gcccaacccg tgccgacgtc tctctcggac
    14941 cttgccgaaa aggcgatcgc acagttctgc gccacgcgcg gactcgaggt ctccaggcgc
    15001 atcgagcccg gcgtcacgat cctcgccgac gcgctcgaga tggagcttct catcagcaat
    15061 cttctcaaga acgccgcaga cgccacggca ggcactgatg acgccgtcat ccgcgcgagc
    15121 ctctccgcag aggacggcac ggccatgctc acgatctcgg acaacggccc gaggctcacc
    15181 gacgagcagt tcgcccgcct caccatcccg ctcaacacct cgaagcccca gggcctcggc
    15241 ctcggactcg tgatcgtgcg ccgcatcgcc gaatccgcac gcggccgcgt actcttcgag
    15301 cggcttcccg aacgaggtct cgccatcacc gtcagcctgc ccgtctgcga acaaaacaac
    15361 aggacttgaa atgccggcaa actcgctttc cctcatccgc atcgtcgacg acgacgcaga
    15421 aatgcgcgaa tcgctcgaat tcctgctctc caccgaaggg tggaagtccc gcagctacgc
    15481 atcggccgag gccttcctcg agaccgacga cgtcatggtg ccgggatgcc tcatcctcga
    15541 catccgcatg cccggcctga gcggcctgca gcttcaggag cttctgaaga agaaggacta
    15601 ttcgcttccc atcctcttca tcaccgcgca cggcgacatc acgatggccg tcgaggcggt
    15661 caagaacggc gccttcgact tcctgcccaa gccgctcgat ggtcaataac ccctgcctaa
    15721 aggcagaggc ttgaaagagc ctttattgac tagcctcagc gcccctcttt cgagaggcgc
    15781 tacgttggtt gggaatgtat ag
//
LOCUS       DBMDTI010000020        15191 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1233_length_15191_cov_2.906030, whole genome shotgun sequence.
ACCESSION   DBMDTI010000020 DBMDTI010000000
VERSION     DBMDTI010000020.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 15191)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15191)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15191
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1233_length_15191_cov_2.906030"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="ACZPB6_02180"
     CDS             <1..695
                     /locus_tag="ACZPB6_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HIR1183320.1"
                     /translation="PKTEADLLKSPEHMADVAQLITESFEKSDRRPRRSRRSKVKPDE
                     TVKTHLKPIAEAVAGIVAEPDPLGKIAVTAKALETKEEEAGKVQVQAEAVELKAEAVE
                     AAAESAVEAAPVVEAAPAVGTEAVVHAPLMGDVAEGLEANLARAGLEQVHTKPELVTF
                     KGYDVVRYSGRPMPVKAEGETEGPLEQVHTRPELCRPVVYEAVRYPGRPYQAPAAALN
                     EEPLVQVHTREA"
     gene            810..1673
                     /gene="gluQRS"
                     /locus_tag="ACZPB6_02185"
     CDS             810..1673
                     /gene="gluQRS"
                     /locus_tag="ACZPB6_02185"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HIR1183321.1"
                     /translation="MSSYRGRFAPSPTGALHAGSLVAAMASFLDARAHDGVWLVRIED
                     IDPPRDIPGAGEHIIRTLARLGLASDEPVLWQHDRHEAYQAALDRLIAQGRVYGCACS
                     RKEVALRAAELGLSAGVYPGTCRLGTHGRPVRAMRFLTTSGTTTFTDRLCGDFTQDVE
                     HEVGDFVLKRADGLWAYQLAAIVDDIHQGVTDVVRGADLLDNTPRQIQLIEALGAQAP
                     RYMHLPLVLNPEGQKLSKQQGATPLDEDDPLGELERAWMHLGFDRLGADSIHAFWDAA
                     LPLWKSRHAVP"
     gene            complement(1758..2381)
                     /locus_tag="ACZPB6_02190"
     CDS             complement(1758..2381)
                     /locus_tag="ACZPB6_02190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR1183322.1"
                     /translation="MALAVKKDMLVTLSVRMADMSGKILEESGPEGLTYLHGHGDIFP
                     KLEAALEGRFPGEGFFIKLEPEDAFGEYDPDSLVMVPVERLGNPETVVRGLVFEGVPG
                     EANDGRRWHVTDIADGMAVLETNHPLAGMALQFEVKVTEVAEPSDEDAVGDDNVVVPG
                     FLSIADRIVSEDEEDESDETMDRMLEDAHPDDSAMARMASAAPRIIR"
     gene            complement(2386..2781)
                     /locus_tag="ACZPB6_02195"
     CDS             complement(2386..2781)
                     /locus_tag="ACZPB6_02195"
                     /inference="COORDINATES: protein motif:HMM:NF017470.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HIR1183323.1"
                     /translation="MRWSRLAMGFCPQFRSGRRLYAQETTGALLAGLFLWLMINPIAD
                     AEFISDEMLTWIGRFVYPLLINLILIFMTVSAARRLHDAGHSGAWALFVMCPGANIFL
                     MLYLLLRPGVSRPTGWRRLEEAAAECGWE"
     gene            complement(2793..3497)
                     /locus_tag="ACZPB6_02200"
     CDS             complement(2793..3497)
                     /locus_tag="ACZPB6_02200"
                     /inference="COORDINATES: protein motif:HMM:NF017470.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HIR1183324.1"
                     /translation="MSVAVNEILSLSFKGRASRGAYWRTLAALAVLAAAGGLALDSLS
                     SLAGGGAAQKGLDLLIWGWLLFLAAFLVSAVVRRLHDISYGAVAAVFLIVPGLQIIVL
                     VILGLMPGKKGLNRFGPDPLAVMTVRDAAPVRRTERSDTVRCPAREVRETLEGEVVEP
                     GPDLEGTIIRERMSAEAEDWASDLIAGAKARIEASPESREGEVAAVRAGLVAARNEGR
                     LTQSAFVRWVQILEGL"
     gene            complement(3627..4784)
                     /gene="bamC"
                     /locus_tag="ACZPB6_02205"
     CDS             complement(3627..4784)
                     /gene="bamC"
                     /locus_tag="ACZPB6_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HIR1183325.1"
                     /translation="MNKRTLRAAALCPALLVLGGCSALGINFTDDKVQYEASNTRANL
                     EVPPDLAPVANENRFEVPSRPGVVSANTEMARAQVAGEATQERGSIVQRTVVAKLMRD
                     GNERWLRVNADAEPLWTVVTDFWPSVGLTIRRQDAKTGYLETEWAENKAKLPQDIIRG
                     TIGKVLDFAYSTGERDQYRCRIERNDDGTSDIFLTHRSMVEVVTGSQSDSTVWQPGPS
                     DPTMEAEMLQRLAIRIDEEFNPDAQPIKKADAAVVEDITKRESRSDVVKAADGTLEAV
                     VLKEPFDRAWRTTGLLIDRMGFELVDRDRAAGNFIVRYLDPAYEAKVKSERGFFKRVF
                     GSDEAIEAPEYRIHLGDEGSVTRIQVLGADGGADTTGSAGSILTLLAEQLR"
     gene            complement(5000..5578)
                     /locus_tag="ACZPB6_02210"
     CDS             complement(5000..5578)
                     /locus_tag="ACZPB6_02210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1183326.1"
                     /translation="MARNDYSECFTRPSPWVLSWKHLLPRQGEVLDVACGPGRHTALL
                     ALEGHRVLACDIDLTGVEALAELPNVTLECRDLEGEPWPWEAERFAGIVVTNYLHRPH
                     FPHYWDSLMPGGVLIMETFTEANMMIWEHPRNPDHYLTEGELIRLAPADARVVAYEEG
                     LTPADTCVARIVLMKHAPAECYAAPLEAGLGI"
     gene            complement(5590..6240)
                     /locus_tag="ACZPB6_02215"
     CDS             complement(5590..6240)
                     /locus_tag="ACZPB6_02215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR1183327.1"
                     /translation="MIENAIQIICVYAIPLIFAITLHEAAHGWAAKKLGDDTAWILGR
                     VTANPVPHIDPVGTVLVPGALVLMSALTGGAGFVFGWAKPVPINPRYFRHFNRDMMLT
                     ALAGPASNLLQAIAWAVVLKLVVMLGISERFVISVCAAGISVNLMLMAFNLIPIPPLD
                     GGRILRGLLPSSGRSLLDRIEPYGMVILVVLMAGGGLGFFARPFLEAGRWLVELVL"
     gene            complement(6387..7232)
                     /locus_tag="ACZPB6_02220"
     CDS             complement(6387..7232)
                     /locus_tag="ACZPB6_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR1183328.1"
                     /translation="MIIDLHMHSNVSDGKLSPADLVRLVHANGVELMALTDHDTMDGV
                     RQASAAAEELGVGFIPGVEISTGWGGRVVHIVGLGVDPDAPGIDAFFSSICTKRDRRG
                     RLIGERFEQLYGIRGAYEGALSLADNKDNLSRTHFARWLMQEGVVKEYQEAFDRFLKT
                     GRPCCVDIDWPGLTEVVDVIHNSGGVAVIAHPGRYSFSESWMLDEFLKAFRDAGGEAI
                     EVCSGSQSRDADVFFADVARRMDFLASSGSDWHAPGTVRPMPGTQPQIPEDLTPVWRI
                     IPRRR"
     gene            7455..8363
                     /gene="hslO"
                     /locus_tag="ACZPB6_02225"
     CDS             7455..8363
                     /gene="hslO"
                     /locus_tag="ACZPB6_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR1183329.1"
                     /translation="MDALQKLLFQRAAVRGETVVLHDEFSRSVEHQHLPLAVRRLAGE
                     VTASALLAAAALEFDGTVLLQIAGDGPIKLLVAEVRPGLLFRVSVTMREDAGEISADA
                     GMTELVNAGGRGRCALILDQTGRDPDTQPYQGVVSLAGETFAEAMTNYFATSEQVETK
                     IKLASDESGVGGLMLQKMPLLGGKALPEDFDEEGWQRLKIFTDTVKSEELLTLDPEEI
                     NRRLFWEESPNVTLEARPEFKCRCSDEGVRSMILSLGREEAEAIIAERGSIDVTCHFC
                     GQVRSFDSADVASLFAGAPAQPRTLN"
     gene            complement(8465..8899)
                     /locus_tag="ACZPB6_02230"
     CDS             complement(8465..8899)
                     /locus_tag="ACZPB6_02230"
                     /inference="COORDINATES: protein motif:HMM:NF016839.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR1183330.1"
                     /translation="MTRIFMLVLAAGICVVGFQLFGPGGAWFRAAELEEALALQNEAN
                     RVQQDRNEALGAELYSLEHRRDAIEERARRELYMVKDDEVLFRLETPGEYEQRARELA
                     SMPDYRSPNIKPGSAPTFSAKRADLYHAPKNLRAPPSRTKRQ"
     gene            complement(8918..10207)
                     /gene="eno"
                     /locus_tag="ACZPB6_02235"
     CDS             complement(8918..10207)
                     /gene="eno"
                     /locus_tag="ACZPB6_02235"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR1183331.1"
                     /translation="MSAIIDVIGREVLDSRGNPTVEAEVWLESGAMGRAAVPSGASTG
                     VREALELRDKDPKRYCGKGVLTAVANVSGEIADALMGLEASDQAYIDRTMIALDGTEN
                     KGRLGANAILAVSMAVARAAAEEAGMPRYRYFGGMGALQMPVPMMNVINGGAHANNNL
                     DLQEFMIIPVGAPSFREALRYGAETFHALKKIINSRGMSTAVGDEGGFAPKCESHEEA
                     IELILEAIKAAGFEAGKDIMIGLDCASSEFFEDGKYVMKKSSGKALTAEEWIAVLEDW
                     VNKYPIISIEDGMAEGDWEGWAKLTAALGKRVQLVGDDLFVTNPAILKEGIEKGVANS
                     ILIKVNQIGTLTETFEAIEMAKRAGYTAVVSHRSGETEDSTIADIAVGLNAGQIKTGS
                     MSRSDRMAKYNQLLRIEEHLAEVAQYPGRDAFYCLRK"
     gene            complement(10306..11163)
                     /gene="kdsA"
                     /locus_tag="ACZPB6_02240"
     CDS             complement(10306..11163)
                     /gene="kdsA"
                     /locus_tag="ACZPB6_02240"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR1183332.1"
                     /translation="MKLCGFDIGLDRPFFLIAGPCVLEGEQMAVDIASAMKEITDELG
                     IPYLFKASYDKANRTSAHSFRGPGMDEGLRILAAVREKVGVPVLTDVHTEAEVPVVAS
                     VVDMLQTPAFLCRQTDFIRACAVSGKPVNIKKGQFLAPGDMLNVVEKAREAASAAGLD
                     PDVFTVCERGASFGYGNLVSDMRSLAIMRQTTAPVVFDATHSVQLPGGNGTCSGGQRQ
                     FVPVLARAAVAAGVSGLFMEAHPTPASAKSDGPNLVPLGRMRELLESLVEIDRLVKAR
                     PFLEDTING"
     gene            complement(11298..12953)
                     /locus_tag="ACZPB6_02245"
     CDS             complement(11298..12953)
                     /locus_tag="ACZPB6_02245"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR1183333.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLAAILESRGLKVTMIKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFISTKMHKPNNFTSGQIYESVLRKE
                     RRGEYLGKTVQVIPHITNEIQEFIQRGAASTFDGKPDVAVVEIGGTVGDIESLPFVEA
                     VRQMSFRVGRNNTCFIHLTLCPWIPSAGELKTKPTQHSVQKLREEGVYPDMLLCRAEH
                     MIPEGERAKISLFSNVPMDCVISVADASTIYEVPLMLHAQHLDDLVCKKLGLETKPAD
                     LSAWEDIVRRIKNPQREVTVAMVGKYVDYADSYKSLNEALIHAGIRTETRVKIRYVDA
                     GQIEEEGAEKILQDVDAILVPGGFGKRGTEGMIKAIEFARENKIPFLGICLGMQMAVI
                     EFARHVCGLGGANSTELDPDTAHPVVALITEWKDRTGSVQKRDESSDLGGTMRLGRQE
                     VPVEKGTLAHKIYGDVVAERHRHRYEVNNAYVAQFEEKGMVISAKTPTEHLPEMMELP
                     GHPFFFAVQFHPEFTSSPRFGHPLFKAYIEAAIAHADEKKAQA"
     gene            complement(13174..14235)
                     /locus_tag="ACZPB6_02250"
     CDS             complement(13174..14235)
                     /locus_tag="ACZPB6_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR1183334.1"
                     /translation="MSEQKHFDDVFQKPEPNSEFSSHGRGDSHWLHAAEKTKTSVLGF
                     AVALTLSFSVIELVGGLWGNSLALIGDAGHMVTDSASLLFALVANKIAQKGADDDHSF
                     GHGRVEVLAAFINGLVMLCVVGWIFFEAVSRIMTPEPVSGLSVMAIAAVGLVINILVA
                     WSLSRDRKNMNTRAALLHVMGDLLGSVAAIVAGAVIYFGGPTIADPILSLVVCCLLLH
                     ATWEILRDSTRVLLDSVPEGVNYFSVGRTIEAIPGVNRVHDLHVWTMSPGHGAIQCHV
                     HIESPECWPKILDVLRRQLHEEFQIDHVTVQPEWDFRGSDEECEVCRYAEFEAACRAD
                     FDDPRAAPAAPVLDAEKLL"
     gene            complement(14383..>15191)
                     /gene="argG"
                     /locus_tag="ACZPB6_02255"
     CDS             complement(14383..>15191)
                     /gene="argG"
                     /locus_tag="ACZPB6_02255"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR1183335.1"
                     /translation="SAFLQKEGFNYRMSAEKAYSTDSNMLGATHEAKDLESLDSNMKI
                     VEPIMGVAFWRDDVEVKPETVTVEFKEGVPVAINGQKFSNPVALMEEANRIGGRHGLG
                     MSDQIENRIIEAKSRGIYEAPGMALLFLAYERLVTGIHNEDTIAEYRINGIKLGRYLY
                     QGRWFDSQALMIRESAMRWVARAVTGEVTIELRRGNDYTIVSTKSPNLTYEPSRLTME
                     KGDSMFTAVDRIGQLTMRNLDITDTRAKLGIYASAGLLAGSGSVAMLTGK"
BASE COUNT         2897 a         4750 c         4821 g         2723 t
ORIGIN      
        1 cccccaagac ggaagccgac ctgctgaaga gccccgagca catggccgac gtggcccagc
       61 tcattacgga aagcttcgag aagtccgacc gccgtccgcg ccgctcccgt cgcagcaagg
      121 tcaagccgga cgaaacagtg aagacccacc tgaagccgat cgccgaagcc gtcgccggca
      181 tcgtcgccga gcccgatccg ctcgggaaga tcgccgtcac cgccaaggcc cttgagacca
      241 aggaggaaga ggcgggaaag gttcaggttc aggctgaagc tgtcgaactc aaggctgagg
      301 ctgtcgaagc cgctgcagaa tccgctgttg aagctgcacc cgtcgtcgaa gccgctcccg
      361 ctgtcgggac cgaagccgtc gtgcacgcgc cgctcatggg cgacgtggcc gagggcctcg
      421 aagccaacct cgcccgcgct ggcctcgagc aggtgcacac caagcccgag ctcgtgacct
      481 tcaagggcta cgacgtcgtc cgctacagcg gccgcccgat gcccgtcaag gctgaaggcg
      541 agaccgaggg cccgctcgaa caggttcata cccgtcccga gctctgccgc cccgtcgtct
      601 acgaggccgt gcgctatccc ggtcgtccct atcaggctcc tgctgctgct ctcaacgaag
      661 agccgctcgt gcaggttcac acccgcgagg cctgatgagc cctgatgact gaccccggtc
      721 agagccccca agggcgccga acgcttcaag gcgtgcggcg ccctttttct tctacaatca
      781 tcttcatctt cacctctgtc tcccgcacca tgtcgtccta ccgaggccgt tttgccccct
      841 ctcccacagg cgcccttcat gcaggcagtc ttgtcgctgc catggcgagc tttctcgatg
      901 cgcgcgccca cgacggcgtc tggctcgttc gcatcgagga catcgacccg cccagggaca
      961 ttccgggcgc aggcgaacac atcatccgca ccctcgcaag gctcggcctt gcatcggacg
     1021 agcccgttct ctggcaacac gacagacacg aagcatatca ggcggccctc gatcgcctga
     1081 tcgcccaagg gcgcgtatac ggctgcgcct gctcccgcaa ggaagttgcc cttcgggccg
     1141 ccgagcttgg tctttccgcc ggcgtctatc ccggcacatg ccgcttggga acgcacggtc
     1201 gccccgtgcg cgccatgcgt tttctgacga cttcaggaac caccaccttc acagaccgcc
     1261 tctgcggcga cttcacgcag gacgttgagc acgaagtcgg cgacttcgtg ctcaagcgtg
     1321 cagacggcct ctgggcctat cagctcgccg ccatcgtcga cgacatacat cagggcgtca
     1381 ccgacgtggt gcgcggcgcc gacctgctcg acaacacgcc tcgtcagatc cagctcatcg
     1441 aggcgctcgg tgcccaagct ccccgctaca tgcatctgcc cttggtactc aaccccgagg
     1501 gacagaagct ctctaagcag cagggagcaa cgccccttga tgaagacgat ccactcggcg
     1561 aactcgagcg tgcatggatg cacctcggat tcgacagact tggtgcagac tcgattcatg
     1621 ccttctggga tgcggcgctc cccctctgga agtcgaggca cgccgtcccg taatgcctcc
     1681 gaagaaggcg cagatgcgac aaaaggcggc gctctcaaga agagcaaccg ccttttgaaa
     1741 ggaaatcgtg gtgacgatca gcggatgatg cgcggcgccg ccgaagccat gcgggccatg
     1801 gccgagtcgt ccgggtgcgc atcctcaagc atacggtcca tcgtctcgtc gctttcgtcc
     1861 tcttcatcct cgctcacgat gcggtctgcg atagacagga agcccggcac gacgacattg
     1921 tcgtcgccca cggcatcctc gtcggaaggt tcggcgactt ccgtcacctt cacctcgaac
     1981 tgaagcgcca tgcctgcgag cggatgattg gtttcgagca ccgccatgcc atcggcaatg
     2041 tcggtcacgt gccagcgtcg gccgtcattg gcctcgccgg gcacgccctc gaagacaaga
     2101 ccacggacca cggtctcggg gttgcccaga cgttcaaccg gcaccatgac gagcgaatcg
     2161 ggatcgtatt ccccgaaggc atcctcgggc tcaagcttga tgaagaagcc ttcgccggga
     2221 aagcggccct caagcgcagc ctcgagcttc gggaagatgt cgccgtgccc gtgcagatag
     2281 gtgagcccct cggggcccga ttcctcaaga atctttccgg acatgtccgc catgcgaacg
     2341 gacagggtta cgagcatgtc tttcttgacg gcaagcgcca tgttttcact cccagccgca
     2401 ctcggcagcc gcctcttcca gacgtctcca acccgttggc cgtgagacgc cggggcgcag
     2461 cagcaggtac agcatcagaa aaatatttgc gcccggacac atcacgaaca gtgcccaggc
     2521 ccccgaatga ccggcatcgt gcagcctgcg cgccgcggac acggtcatga agataagaat
     2581 gagattgatg aggagaggat agacgaagcg gccgatccaa gtcagcatct cgtcgctgat
     2641 gaactcggcg tcggctatcg gattgatcat gagccagaga aaaagccccg ccagaagcgc
     2701 gcccgtcgtc tcctgggcgt aaagcctcct cccgcttcgg aactgcgggc agaatcccat
     2761 ggcgagcctg ctccagcgca tgatgccccg cctcagagcc cctcgaggat ctgcacccat
     2821 cgcacgaacg cagactgggt gagacgcccc tcgttgcgcg cggcaacaag ccccgccctg
     2881 acggcggcaa cctcaccctc acggctttca ggcgaagctt cgatgcgcgc cttcgctcct
     2941 gcgatgagat cggaggccca gtcttcggcc tcggcactca tgcgttcgcg aatgatcgtg
     3001 ccctccagat cgggccccgg ctccacgacc tcgccctcaa gcgtctcgcg cacttcgcgc
     3061 gcaggacacc tgacggtgtc gctccgttcg gttcgacgca cgggcgcggc atccctcacc
     3121 gtcatcacgg caagcggatc cggaccgaag cggttgagcc ccttcttgcc cggcatcaga
     3181 ccgaggatga cgagcacgat gatctgaagg cctggcacga tcagaaatac cgccgcaacg
     3241 gccccatagc tgatgtcgtg cagccggcgc acgacggccg acaccagaaa ggcggccaga
     3301 aagaggagcc acccccatat gagaagatcg agtcccttct gcgcggcgcc tccgcccgca
     3361 aggctcgaaa gcgaatcgag cgccaatccg cctgccgccg caagcacggc cagcgccgca
     3421 agcgtgcgcc agtaggcacc gcggctcgca cgtcccttga aggacagaga gaggatctca
     3481 ttgacggcca cactcatcgt tcatcaatcc ctgaaggcaa gcggtccaaa acttgaaaag
     3541 ccccggagga cctctacgag agagtccgga tccgaggctt tgaacgcacc tgcggcaagc
     3601 cgcaggccgg atgcgccttt cggcggtcag cggagctgct cggcgagaag cgtcaggatg
     3661 cttccggcgg aaccggtcgt atcggcgcca ccatcggcac cgagcacctg aatgcgtgtc
     3721 acggagcctt catcgcccag atggatgcgg tattcgggcg cctcaatggc ctcgtcggaa
     3781 ccgaagacac gcttgaagaa accgcgttcg gacttgacct tggcttcata ggcggggtca
     3841 aggtagcgga cgatgaagtt gccggcggca cggtcgcggt cgacaagctc aaagcccatg
     3901 cggtcgatga ggagccccgt ggtgcgccag gcgcggtcaa acggttcctt gaggacgact
     3961 gcctcaagcg tgccgtcggc agccttcacg acatcgctgc gggactcgcg cttggtgatg
     4021 tcctcaacaa cggccgcatc ggccttcttg atcggttggg catcgggatt gaactcctcg
     4081 tcgatgcgga tggccagacg ctggagcatt tcggcctcca tcgtcggatc ggagggaccg
     4141 ggctgccaga ccgtggagtc gctctgagag ccagtgacga cctcaaccat cgaacgatgc
     4201 gtcaggaaga tgtcactcgt gccgtcgtca ttgcgctcga tgcggcaacg gtactgatcg
     4261 cgttcgcccg tggagtaggc gaagtcgagc accttgccga tcgtgccgcg gatgatgtcc
     4321 tgcgggagct tggccttgtt ttcggcccat tcggtctcaa ggtagccggt cttggcgtcc
     4381 tgacgacgaa tcgtgaggcc gacgctcggc cagaagtctg tgacgacggt ccagagcggc
     4441 tcggcgtcgg cgttgacgcg aagccagcgc tcgttgccgt cgcgcatgag cttggcaacg
     4501 accgtgcgct gcacgatgga gccgcgctcc tgagtggctt cgcctgcgac ctgagccctt
     4561 gccatctcgg tgttggcact gacgacgccc ggacgggacg gcacctcgaa gcggttttca
     4621 ttggcgacag gcgcaagatc aggcggcact tcgagattgg cacgcgtatt ggaggcttcg
     4681 tactggacct tgtcgtccgt aaagttgatg ccgagagccg agcagccgcc aaggaccagc
     4741 agagcaggac agagagcggc ggcacgcagc gtacgcttat tcatcggggt aatcctttgc
     4801 tagagaaacc aatgcggaac cgtgcgtcga acacaggccg ggaaggcccc gtccggaata
     4861 gctccgcgaa aaaacgtctc cgccgtgcga agacctccga ccgcagggcg gccgacaata
     4921 tcttgaagag tttagcaaac ttgaccgcct caaggcgctt ttgaaagcgt gcgattgcgg
     4981 taattacgga ggtccgccct cagataccga gccccgcctc aagcggtgcg gcgtagcact
     5041 ccgcaggcgc atgcttcatg aggacgattc tcgcgacgca cgtgtcggcg ggagtgagtc
     5101 cctcctcgta ggcgacgact cgcgcatcgg caggagccag gcgaatgagc tccccctccg
     5161 tcagatagtg gtcgggattg cgcggatgct cccagatcat catgttggct tccgtgaagg
     5221 tctccatgat gaggacgccg cccggcatca ggctatccca gtaatgcgga aagtgcggcc
     5281 tgtgcaggta gtttgtcacg acgatgcccg caaaccgctc cgcctcccaa ggccagggct
     5341 caccctcgag atccctgcac tcgagcgtca cattcgggag ctccgcaagg gcctcgacgc
     5401 ccgtcaggtc gatgtcgcag gcaagaaccc tgtggccctc aagagccaga agcgccgtat
     5461 gccttccggg cccgcaagcc acgtccagca cctcgccctg acggggcagc aggtgcttcc
     5521 agctaagcac ccaaggcgaa ggcctcgtga agcattcgga atagtcgttg cgcgccatgg
     5581 cacctcccgt cacagcacga gctcgacgag ccagcgtccc gcctcgagga agggacgagc
     5641 gaagaacccg aggccgccgc ccgccatcag cacgacgagg atcaccatgc cgtagggctc
     5701 gatcctgtcc agaagactcc tgccgctcga gggcagaagt cccctcagga tgcggccgcc
     5761 gtcgagcggc ggaatcggaa tgaggttgaa ggccatcagc atcaggttga ccgagatgcc
     5821 ggccgcgcac acgctgatca cgaaccgctc ggagatcccg agcatcacga cgagcttgag
     5881 caccacggcc caggcgatcg cctgcaaaag gttggaggcg ggccccgcaa gcgccgtgag
     5941 catcatgtcg cggttgaagt gcctgaagta acgcggattg atcggcacgg gcttggccca
     6001 gccgaagacg aagcccgcgc cgcccgtgag agcggacatc aggacaagcg ccccgggcac
     6061 gagcacggtg ccgacgggat cgatgtgggg aacgggattg gccgtcacgc ggccgagaat
     6121 ccaggcggtg tcgtcgccaa gcttcttcgc cgcccagccg tgcgcggcct catggagcgt
     6181 gatggcgaag atgagcggaa tggcgtagac gcagatgatc tgaatggcgt tttcaatcat
     6241 ggagggagca aaggcttcgg ggtgcagtgg aaagactgcg cccggatgga tttacggaag
     6301 gggttcaatc gtgaaagtct gccacaaacc cgcgccgtgc acgccgaccg acccgcgtaa
     6361 atgtcgacag actgtttgaa aacgtgtcaa cggcgacgcg gaatgatgcg ccagacgggc
     6421 gtgagatcct cgggaatctg cggctgcgtg ccgggcatgg gacgcacggt gccgggcgca
     6481 tgccagtcgg agcccgaact cgcgagaaag tccatgcggc gcgcgacgtc cgcaaagaag
     6541 acgtccgcat cgcgggactg ggatcctgag cagacctcaa tcgcctcgcc gcccgcgtcc
     6601 ctgaaggcct tgagaaactc gtcgagcatc caggactcgc tgaagctgta gcgtccggga
     6661 tgtgcaatga ccgcgacgcc gcccgaattg tggatgacgt cgaccacttc ggtgagtccc
     6721 ggccagtcga tgtcgacgca acaaggcctc cccgtcttga gaaagcggtc gaaggcctcc
     6781 tgatactcct tcacgacgcc ctcctgcatg agccagcgtg cgaagtgagt gcgggaaagg
     6841 ttgtccttgt tgtcggcaag cgaaagcgcg ccctcatagg cgccgcgtat gccgtagagc
     6901 tgctcgaacc gttcgccgat aaggcggccg cgccggtcgc gcttggtgca gatgctgctg
     6961 aagaatgcgt cgatgcctgg cgcatcagga tcgactccga gccccacgat gtgcacgacg
     7021 cgtcctcccc atcccgtcga gatctccacg ccgggaatga acccgacgcc gagctcctcg
     7081 gcggcggccg atgcctgcct gacgccgtcc atcgtatcgt ggtccgtcag cgccatgagc
     7141 tccacaccgt ttgcatgcac gagacgcacg aggtcggcgg gcgaaagctt cccgtcggaa
     7201 acattggagt gcatgtgcag gtcgatgatc atcagaagag ccttgcgcgg gaagtacgta
     7261 gtaatttttt gattatacgg ccgcgcgttc aggaccgctt gaggcggaca tggtattcgg
     7321 ctcacttgaa aaggcgtccc cttgccccca tataaagcgt attgcttcaa ttgcgcgcgg
     7381 gcccgctccg gccgcggcgg gcgcatgatc ccgcctgcgc cgcccgcact gcgcgcgaac
     7441 acacaggaat tcatatggac gcccttcaga aactgctttt ccagcgtgcc gccgtccgcg
     7501 gcgagaccgt cgtgcttcac gacgaattca gccgctcggt cgagcatcag catctgcccc
     7561 tcgccgttcg ccgcttggcg ggcgaagtga cggcctccgc acttcttgcg gcagccgccc
     7621 tcgagtttga cggcacggtg cttctgcaga ttgcgggcga cggtcccatc aagctactcg
     7681 tggcggaagt ccgtcccggc ctcctcttcc gcgtctccgt gacgatgcga gaggacgctg
     7741 gcgagatcag cgccgacgca ggcatgaccg aactcgtcaa cgcaggcggc cgcggtcgct
     7801 gcgcgctcat tctcgaccag acggggcgcg atcccgacac ccagccctat cagggcgtgg
     7861 tgagtcttgc aggtgaaacc ttcgccgaag ccatgacgaa ctacttcgcg acgagcgaac
     7921 aggtcgaaac gaagatcaag ctcgcaagcg acgaatccgg cgtcggcggt ctgatgctgc
     7981 agaaaatgcc gcttctcggc ggcaaggccc ttcccgagga cttcgacgag gaaggctggc
     8041 agcgtctcaa gatcttcacc gacaccgtca agagcgaaga gcttctcacg ctcgatcccg
     8101 aagagatcaa ccgccgtctc ttctgggagg aatccccgaa cgtcacgctc gaagccaggc
     8161 ccgaattcaa atgccgctgc tccgacgaag gcgtgcggag catgatcctt tctctgggcc
     8221 gggaggaggc cgaagccatc atcgccgaac gcggctcgat cgacgtcacc tgccacttct
     8281 gcggtcaggt acgctccttc gacagcgctg atgttgcctc tcttttcgcg ggcgctcccg
     8341 cccagcccag aacgctcaac tgatcaggtc ttccagggag gaaagcttca ctttcctccc
     8401 aacttcacga gcaaagaaaa aacgcacggt ccgattgcgg cggatcgtgc gtttttttct
     8461 tgccttactg gcgcttcgtg cgggacggag gcgcgcgaag gttcttgggc gcatggtaga
     8521 ggtcggcgcg cttcgcggag aacgtcggcg cggagcccgg cttgatgttg ggagagcggt
     8581 agtcaggcat gctcgcgagc tcgcgggccc tctgctcata ttctcccggc gtctcaaggc
     8641 ggaaaagcac ctcgtcgtcc ttcaccatgt agagctccct gcgggcgcgc tcctcaatgg
     8701 cgtccctgcg gtgctcaagc gaataaagct ccgcgcccag agcctcgttg cggtcctgct
     8761 gaacgcggtt ggcctcattc tgaagcgcga gcgcctcctc aagctcggcg gcacgaaacc
     8821 atgcgccgcc gggcccgaag agctggaacc ccacgacgca gatgcctgcc gccagcacga
     8881 gcatgaagat gcgcgtcatc gcttcaaggc gtccgtctta cttgcgaagg cagtagaagg
     8941 cgtcgcggcc gggatactga gccacttcgg cgaggtgttc ctcgatgcga agaagctggt
     9001 tgtacttggc catgcggtcg gagcggctca tggaaccggt cttgatctga ccggcgttga
     9061 gaccaacggc gatgtcggcg atcgtgctgt cttccgtctc gcccgagcgg tgggagacga
     9121 cggcggtgta gcccgcgcgc ttggccatct caatggcttc aaacgtttcg gtaagcgtac
     9181 cgatctggtt gaccttgatg aggatggagt tcgcaacccc cttttcaatg ccttccttga
     9241 ggatggcggg attcgtcacg aagaggtcgt cacccacgag ctggacgcgc ttgccgagag
     9301 cggccgtgag cttggcccag ccttcccagt cgccttcagc catgccgtct tcgatcgaga
     9361 tgatcgggta cttgttgacc cagtcctcga gaacagcgat ccattcctcg gccgtgaggg
     9421 ccttgccaga ggacttcttc atgacgtact tgccgtcttc gaagaattcg ctcgaggcgc
     9481 agtcgagacc gatcatgatg tccttgcctg cttcaaagcc ggcagccttg atggcttcga
     9541 ggatgagctc gatggcttct tcatggcttt cgcacttggg ggcgaagccg ccctcgtcac
     9601 cgacggccgt ggacatgccg cgggagttga tgatcttctt gagggcatgg aacgtctcgg
     9661 caccgtagcg aagggcttca cggaaggacg gagcgcccac cggaatgatc atgaactcct
     9721 gcaggtcgag gttgttgttg gcgtgagcac cgccgttgat gacgttcatc atcgggacgg
     9781 gcatctggag ggcacccatg ccgccgaagt agcggtagcg cggcatgccg gcctcttcgg
     9841 cggcggcgcg ggccacggcc atggagacgg cgaggatggc attggcgccg aggcggccct
     9901 tgttttcggt gccgtcgagc gcaatcatcg tgcggtcgat gtaggcctgg tcggaggcct
     9961 caaggcccat gagggcgtcg gcgatttcgc cggagacgtt ggcgacggcg gtcagcacgc
    10021 ccttgccgca gtagcgcttc ggatccttgt cgcgaagctc gagagcctcg cgcacgcccg
    10081 tggaggcgcc ggacggcacg gcggcgcggc ccatggcgcc gctttcgagc cagacttcgg
    10141 cttcaacggt cggattgccg cggctgtcga gaacttcacg gccgatcaca tcaataattg
    10201 cgctcatctt ttgtcctttc gtttggatga aaaagggatg tcaaggaaag aagattcccg
    10261 cggttcgacg acgttgcgtg cggatcgcgg gcacatcaga atattttacc cgttgatcgt
    10321 atcttcaagg aaggggcggg ccttcacgag gcggtcgatc tccacaaggc tctcgaggag
    10381 ctcacgcatg cggccgagtg gaacgaggtt gggaccgtcg ctcttggcgg aggcgggcgt
    10441 cggatgggct tccatgaaga ggccggagac gcccgctgcg accgcagctc gcgcgagcac
    10501 gggaacgaac tggcgctgac cgccggagca cgtgccgttg ccgccgggaa gctgcacgga
    10561 atgtgtggcg tcgaagacca cgggagccgt cgtctggcgc atgatcgcaa ggcttcgcat
    10621 gtcggacacg aggttgccgt aaccgaacga ggcgccgcgc tcgcagaccg tgaagacgtc
    10681 ggggtcaagg cctgcagccg aggcggcctc acgggccttc tcgacgacgt tgagcatgtc
    10741 gcccggtgca aggaactggc ccttcttgat gttgacgggc ttgccagaaa ctgcgcaggc
    10801 gcggatgaaa tccgtctgac ggcagaggaa ggcgggcgtc tggagcatgt cgacgacgga
    10861 ggcgacgacg gggacctctg cttcggtatg cacgtcggtg agaacgggca cgccgacctt
    10921 ctcgcgaacg gcggcgagaa tgcgaaggcc ctcgtccatg ccgggaccgc ggaaggaatg
    10981 ggcggacgtg cggttcgcct tgtcatagct cgccttgaag agatacggaa tgccgagttc
    11041 gtccgtgatc tccttcatgg cggaggcgat gtcgacggcc atctgctcgc cctcgagcac
    11101 gcaggggccg gcgatcaaga agaacgggcg gtcgagcccg atgtcaaagc cgcagagctt
    11161 catgtttgtt gtcctcttgc aggagggcgc gccccggaaa agtcgtccag acaagacggc
    11221 gcccgaaatg aaaaaggcag ccccacgaac tgcagtgccc gttgagctgc cttgcgtcta
    11281 ggtcggatca gtccggctta ggcctgagcc ttcttttcat cggcgtgcgc gatcgcggcc
    11341 tcaatgtagg ccttgaagag cggatggccg aagcgggggc tcgacgtgaa ttccggatgg
    11401 aactggacgg cgaagaagaa gggatggccc gggagctcca tcatttcggg gaggtgctcc
    11461 gtcggagtct tggcggaaat gaccatgccc ttttcctcga actgggccac ataggcgttg
    11521 ttcacctcat agcggtgacg atggcgttca gccacgacgt cgccgtagat cttgtgggcg
    11581 agcgtaccct tttcgacggg aacttcctga cggccgaggc gcatcgtgcc gccgaggtcg
    11641 gacgactcgt cgcgcttctg gacgctgccc gtgcggtcct tccactcggt gatgagggcg
    11701 acgacgggat gggccgtgtc gggatcgagc tcggtggagt tggctccgcc gagaccgcac
    11761 acgtgacggg cgaactcgat gacggccatc tgcatgccga ggcagatgcc gaggaacggg
    11821 atcttgtttt cacgggcgaa ctcgatggcc ttgatcatgc cttccgtgcc acgcttgccg
    11881 aaacctcccg ggacgaggat cgcatcgacg tcctgaagga tcttctcggc gccttcctct
    11941 tcgatctggc cggcgtcgac gtagcggatc ttgacgcggg tctccgtgcg gatgccggca
    12001 tggatcagcg cttcgttgag ggacttgtag ctgtcggcgt agtcgacgta cttgccgacc
    12061 atggcgaccg tgacctcgcg ctgcgggttc ttgatgcgac ggacgatgtc ctcccaggcg
    12121 gagagatcgg cgggcttcgt ctcaaggccg agcttcttgc agacgaggtc gtcaagatgc
    12181 tgggcgtgga gcatgagcgg cacttcgtag atcgtggagg cgtcggcgac gctgatcacg
    12241 cagtccatcg gcacgttgga gaagagcgag atcttggcgc gctcgccttc cgggatcatg
    12301 tgctcggcgc gacagaggag catgtcggga tagacgccct cttcgcggag cttctgaacg
    12361 gagtgctggg tcggcttcgt cttgagctcg ccggcggacg gaatccacgg gcagagcgtc
    12421 aggtggatga agcaggtgtt gttgcggccc acgcggaagg acatctggcg gacggcctcg
    12481 acgaacggca gggactcaat gtcgccgacc gtaccgccga tttcaacgac ggccacgtcg
    12541 ggcttgccgt caaaggtgct ggcggcgccg cgctggatga actcctgaat ttcgttcgtg
    12601 atgtgcggga tcacctgaac ggtcttgccg aggtattcgc cgcgacgttc cttgcgaagc
    12661 acgctctcgt agatctgacc cgacgtgaag ttgttgggct tgtgcatctt cgtggagatg
    12721 aagcgttcgt agtgaccgag gtcgaggtcg gtttccgccc cgtcttccgt cacaaaaact
    12781 tcaccgtgct ggaaaggcga catcgtgccc gggtcgacgt tgatatacgg atcgagcttg
    12841 atcatggtca ccttcagacc acgagattcc agaatagccg ctaacgaagc ggcggcgatg
    12901 cccttgccga gagaagagac aacgccgccg gtcacaaata cgtacttggt catgaagaag
    12961 agccccacgc aaggaggtac aggaaaaaat taaagccgag attatacctg aagttcggca
    13021 taccgcccga aaaacgtctc cgcttccgcg ccgcctccga atcaggccga ttattcattg
    13081 tacgagaatt ctcccgacgc cgacacgact ttttgccggg actcccgaag ggcttccccg
    13141 cccggcctac gcgtacggag gaagccctcg ggctcagaga agcttctccg catcgagcac
    13201 gggcgccgca ggcgcggccc tcggatcgtc gaagtccgcg cggcaggcgg cctcgaactc
    13261 cgcatagcgg cagacctcgc actcttcgtc gctgccgcga aagtcccact cgggctgcac
    13321 ggtgacgtgg tcgatctgga actcctcatg aagctgcctg cgcaacacgt cgaggatctt
    13381 gggccagcac tcggggcttt cgatgtgcac gtggcactgg atggcgccgt gaccggggct
    13441 catcgtccag acgtgcaggt cgtgcacgcg gttgacgcct gggatagcct cgatcgtgcg
    13501 accgacgctg aaatagttga cgccttcagg cacgctgtca agaagaacgc gcgtcgagtc
    13561 gcggaggatc tcccaggttg catgcagaag caggcagcag acgacgagcg agagaatcgg
    13621 gtcggcaatc gtcggaccgc cgaagtagat gacggcgccc gcaacgatgg ccgccacgga
    13681 gccgagcagg tcgcccatca cgtgcagaag cgccgcgcgc gtgttcatat tcttgcggtc
    13741 tcgggagagg gaccaggcaa cgagaatgtt gatcaccaga cccacagccg cgatcgccat
    13801 gacggaaagg ccggagacgg gttcgggcgt catgatgcgc gagacggcct cgaagaagat
    13861 ccatcccacg acgcagagca tcacgagacc gttaatgaag gcggcaagca cctcgacgcg
    13921 gccgtgacca aaggagtggt cgtcgtccgc ccccttctgg gcgatcttgt tggcaactaa
    13981 cgcgaaaagc aggctcgccg agtcggtcac catgtgcccg gcgtcgccga tcagagcaag
    14041 agaattgccc cagaggccgc ccacaagctc gatgacggag aagctgagcg tgagcgcaac
    14101 cgcgaagccc agcacgctcg tcttcgtctt ttcggccgca tgaagccagt ggctgtctcc
    14161 tcggccgtga ctcgagaact cggagttggg ctcgggcttt tgaaaaacgt cgtcgaaatg
    14221 cttctgttcg gacatgatga atggtgccgt tgttgacctc tgctgcgcgc agacaagcgc
    14281 aaggcccgga catcctcagg gctgtccggg cctttgttct gatttgagac ggattataaa
    14341 gcctccgcct ccgtcaggca tgccctcggg cggcgacagg acttacttgc cggtgagcat
    14401 cgccaccgag ccgctgcctg cgagaagccc cgcgctggcg tagatgccga gcttggcgcg
    14461 cgtgtccgtg atgtcgaggt tgcgcatcgt gagctgaccg atgcggtcga cggcggtgaa
    14521 catcgagtcg cccttctcca tcgtgaggcg ggacggttcg tacgtcaggt tcgggctctt
    14581 ggtggagacg atcgtatagt cattgccgcg gcgcagctcg atcgtcactt cgccggtgac
    14641 ggcacgggcg acccagcgca tggcgctttc gcggatcatc agcgcctgac tgtcgaacca
    14701 gcggccctga tagaggtagc ggccgagctt gatgccgttg atgcggtatt cggcgatcgt
    14761 atcctcattg tgaatgccgg tgacgaggcg ttcgtaggca aggaagagaa gcgccatgcc
    14821 cggagcttca tagatgccgc gggacttggc ctcgatgatg cggttctcga tctggtcgga
    14881 catgccgaga ccgtgacggc cgccgatgcg gttggcctct tccatgagag cgacgggatt
    14941 cgagaacttc tgcccattga tggcgacggg aacgccctcc ttgaattcaa ccgtgaccgt
    15001 ttcaggcttg acttcaacgt cgtcgcgcca gaaggcgacg cccatgatcg gctcgacgat
    15061 cttcatgttg gaatcgaggc tttccaggtc cttggcctca tgcgtggcgc cgagcatgtt
    15121 cgagtcggtc gagtaggcct tctcggcgct catgcggtag ttgaagccct ccttctgaag
    15181 gaaggcagac a
//
LOCUS       DBMDTI010000021        14786 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1270_length_14786_cov_3.632618, whole genome shotgun sequence.
ACCESSION   DBMDTI010000021 DBMDTI010000000
VERSION     DBMDTI010000021.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 14786)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14786)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14786
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1270_length_14786_cov_3.632618"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="ACZPB6_02260"
     CDS             <1..1005
                     /locus_tag="ACZPB6_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1183336.1"
                     /translation="AKQAMREAGVPCVPGSDGALPDDPQEILRIARQIGYPVIIKAAG
                     GGGGRGMRVVRTEAALITSVNMTREEARVAFGNPTVYMEKFLENPRHIEIQVLSDNFQ
                     NAVYLGERDCSMQRRNQKVLEEAPAFGIPRKLIDRLGNRCVEACRRIGYRGAGTFEFL
                     YENGEFYFIEMNTRVQVEHPVTEMITGVDIVREQIRIAAGERLSVRQRDITMRGHAIE
                     CRINAEDPFTFVPSPGRVTAWHLPGGPGIRIDTHVFSGYTVPPYYDSMIGKIIAHGDT
                     REQAIARMRVALSELAVEGIKTNTKLHRELLADERFFQGGTSIHYLEEKLRSRAEAHK
                     "
     gene            1069..1965
                     /gene="prmA"
                     /locus_tag="ACZPB6_02265"
     CDS             1069..1965
                     /gene="prmA"
                     /locus_tag="ACZPB6_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR1183337.1"
                     /translation="MRQIKILADERSAEALSDMLMDAGALSATIEDADAESTDEKPLY
                     GEPGLEPDNCAWPRSIVSILADDDFDLETSLAIAVKALKCEKPELLGTEEVEDADWVR
                     ITQAQFQPTQVSDRLWIVPTWHDLPNEDAINVRLDPGVAFGTGSHPTTHLCLQWLDAN
                     VKATDRVLDYGCGTGILAIAAKLVGAAEVAGTDIDPQAVEAAGDNALMNHVEAKFYLP
                     DDMPEGTYDIVVANILANPLKLLAPALLGRVKQGGALVLSGVLAKQAEEVIAAYQAID
                     PAVKLSVWRQEEDWVCIAGRRA"
     gene            1996..2823
                     /locus_tag="ACZPB6_02270"
     CDS             1996..2823
                     /locus_tag="ACZPB6_02270"
                     /inference="COORDINATES: protein motif:HMM:NF023334.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HIR1183338.1"
                     /translation="MAKVLRCPACGALWRVKDDFAADMLHCTECQACFSADKTESLSV
                     PDEKLDARLQQAARRQAVMQEEAREGEAVMSRIADSLTDFDARTEAPEAPPAAKPAPR
                     APESAERSHGVLWTLVTLLALVVLSAAGLLLGHKTVLGTMPQLRPVYEEVCMKAPCPG
                     FVWQNAAAFKVTAEIERPLETADDADREVARRLPVVTARLSNTSELPQALPILEMKLL
                     DAAGETLAQRVLEPADYGFGGRDAVAPGADVAVRLTVTTPLPYDASSAVVKAVSALQ"
     gene            2882..3814
                     /locus_tag="ACZPB6_02275"
     CDS             2882..3814
                     /locus_tag="ACZPB6_02275"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1183339.1"
                     /translation="MSVIISGSIAYDTVFTHEGRFADTLRAEALDRLNVTLQADSMRR
                     TYGGCAANIAYSLKQLGGDPLVWTAVGRDADDYLRHLERQGIRTDGVTVMPNEWTAQC
                     MITTDRDGNQLATFSPGAMAHADRLPWPDDTGIVCGILAPSVRSTLLAHAKAFVSHGI
                     PFIFDPGQTTPLFSGDELLALTRAAACVAFSDYEALMIEEHTSLSPAELSRLTGAVFC
                     THGSQGSSVWQKGEETVIPAPKVEAADPVGAGDAYRGGLLWGMERGLDAVSCAIAGTV
                     MGAAKATAAGPSYRINADLVEKAVRKGMPFDCRS"
     gene            complement(3877..4437)
                     /locus_tag="ACZPB6_02280"
     CDS             complement(3877..4437)
                     /locus_tag="ACZPB6_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR1183340.1"
                     /translation="MPTLLTGLIQFLFTSVMSILGVLLLLRAWVYVWAFSPRHPIVQL
                     ARRATDWLVEPLSKLIPRSGSYDWPSLVAALVAAVIMVLMQRVLTGMPYTPIGLVIAP
                     LATVLRWALEMMSWGVFIWVVMTWLNPQSPMTYALGTLVDPFVRPVRKIIPMFGRFDL
                     SPIVVIILANVLLMLVTPISHGFIPL"
     gene            complement(4492..5076)
                     /gene="ampD"
                     /locus_tag="ACZPB6_02285"
     CDS             complement(4492..5076)
                     /gene="ampD"
                     /locus_tag="ACZPB6_02285"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HIR1183341.1"
                     /translation="MTSPDRAFDGWLPGVVRRLSPHFNERPEGALVSLAVLHFISLPA
                     GRFGGEDVDALFMGTLDAMNRPEYESLRGLRVSSHFFVRRTGEVRQYVSVLDRAWHAG
                     VSSFEGHTGCNDFSVGIELEGTGETPYEDAQYLALGELLGTLTRVLPVEAVTGHEHIA
                     PGRKQDPGPSFDWDRVREMVPGRVRIVTEPQVMP"
     gene            complement(5073..5354)
                     /locus_tag="ACZPB6_02290"
     CDS             complement(5073..5354)
                     /locus_tag="ACZPB6_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HIR1183342.1"
                     /translation="MGRILFFVALAVAMWVAWTLSRRRNSLDNEERRELKRLRAKERE
                     AQGKSREPSGEVMVKCDHCGLYFPKREAVLSKERVYCSKRCRDAARDGQ"
     gene            complement(5364..6176)
                     /locus_tag="ACZPB6_02295"
     CDS             complement(5364..6176)
                     /locus_tag="ACZPB6_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HIR1183343.1"
                     /translation="MTILTICTIIAAILYLGSGMAIIGSMKGEGSGASSLIRWPVLAG
                     LVMQGYALHGEMFGEGAVNFGFGFAMSEMFFFAVIILLIETWIHRLHGQFGIVLIAAA
                     VGTLSPLLFTGQVIPSVEWTTIFRWHLLLAIAAYAFLMIAFVHAVLMALINRRLKSPA
                     VGETSKFLDSMPALVVMERIFFRIVVVGFLFITLTLIVGALATQEAKGVYFLFDHKTI
                     LTWLAWVFFGILLAGRRVAGWRARTALNWFWAGFVVFVVAYFGYSFIQELVR"
     gene            6567..7958
                     /gene="ffh"
                     /locus_tag="ACZPB6_02300"
     CDS             6567..7958
                     /gene="ffh"
                     /locus_tag="ACZPB6_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016723786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR1183344.1"
                     /translation="MLDSLSQRLSKIVKTMRGQARLTEENTKDMLREVRIALLEADVA
                     LPVVREVLARIKEKAMGEEVVGNLNPGQALVGVVQRELTAIIGGDVPAAERDINLHTQ
                     PPAVILLAGLQGAGKTTSAGKLAKYLIETKRKKVLTVSADVYRPAAIEQLKTVTEQAG
                     AEWFPSTVGQKPLEIAAAAIDHARRKFFDVLIVDTAGRLAIDEAMMQEVAELNTFLKP
                     VETLFVIDAMLGQDAVNTAKAFNERLPLTGVVLTKIDGDSRGGAALSVRHVTGKPIKF
                     VGTAEKLTGFDAFDPEAMASRILGMGDIVGLVKDVQKSINIAEAEKLAQKISQGDKFD
                     LNDFRAQLAQMNNMESFSSILEKLPAQFQEAASKVNDEEAKKSVRRTMGIIDSMTPFE
                     RRKPDLIKASRKKRIAAGAGVPVQEVNRLLKQFEQTQDMMKRMKRGGLGKMMRALGQF
                     GRGGFPGMGSFGR"
     gene            7967..8554
                     /gene="ruvC"
                     /locus_tag="ACZPB6_02305"
     CDS             7967..8554
                     /gene="ruvC"
                     /locus_tag="ACZPB6_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR1183345.1"
                     /translation="MRILGLDPGLNRTGWGVIDAASGRFVRVDSGMIRVPEGELSARL
                     GHIVRELSKVIERTKPDLASAERVFVNVNPQSTLRLGQARGAALVAADLAGLPVEEFT
                     PSEIKEAVCATGRADKPMVQRMIGMLLGIEGDIQADEADALACAVCCANSIRMRAFEK
                     AGSTTRTYATPRRGRSAAGARSAWTALFTKEGKTS"
     gene            8551..9126
                     /gene="ruvA"
                     /locus_tag="ACZPB6_02310"
     CDS             8551..9126
                     /gene="ruvA"
                     /locus_tag="ACZPB6_02310"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR1183346.1"
                     /translation="MIGRLHGKLIEKTPPQVLVDVGGVGYEVDVPMSTFCNLPAEGSE
                     ITLLTHFIVREDAQLLYGFATAAERQTFRALIRISGVGPRIALAVLSGMSTQDLADAV
                     EQGNATLLTRVPGIGKKTADRLVLELKGKLAGNAFAPAGGAASAAQADILSALMALGY
                     SEREAQASVRALPAEVTVSEGIRLALKALAR"
     gene            9139..10176
                     /gene="ruvB"
                     /locus_tag="ACZPB6_02315"
     CDS             9139..10176
                     /gene="ruvB"
                     /locus_tag="ACZPB6_02315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR1183347.1"
                     /translation="MLEEERIIDGRTASPNEENVDRALRPSLLNEYVGQEAIREQLQI
                     FIAAAKRRGEPLDHLLLFGPPGLGKTTLAHIVAHEMGVNLRQTSGPVLERPGDLAAML
                     TNLEPHDILFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSIKIDLPPFTLIG
                     ATTRAGSLTNPLRARFGIVNQLQFYTPEELSRIVRRSAGLLNVGIDETGAFEIARRSR
                     GTPRVANRLLRRVRDYAEVKHDGRITKEVAAAALKMLNVDPEGLDMIDTHFLRTIIEK
                     FSGGPVGIENLAASVGEDRDTLEDVVEPYLIQQGFVQRTPRGRVATPLAWQHFRLAPP
                     TPVARDLFGSY"
     gene            10185..10886
                     /locus_tag="ACZPB6_02320"
     CDS             10185..10886
                     /locus_tag="ACZPB6_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183348.1"
                     /translation="MPFSPILSVLVCLTSAAVFAACVLFIRSARHRAILHELLAHPAR
                     QALAVFAAATLGAALLSAMPIDRDGRTDTLLQATIEATGAGLPEMSASAPFAVVDDHG
                     RQLMHAGERLSSETPPLIHIIKYTLVALIAGGVTSFASILFTAAIIGARKGQNLATGI
                     TMLSSFYWRPGMLTGTALWMILTWLLLLWPDWHVLGTTAAGTDVMAMVAGLLPWGTCA
                     AFWYLVFAYWRNIRA"
     gene            11009..11428
                     /gene="ybgC"
                     /locus_tag="ACZPB6_02325"
     CDS             11009..11428
                     /gene="ybgC"
                     /locus_tag="ACZPB6_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HIR1183349.1"
                     /translation="MNHIRFPVRVYWEDTDAGGIVYHANYLKFMERARSDMLRRIGFG
                     QNDQRGREDGVLFVVASLNIAYHRAAVLDDDLEVITSIEKLGRASLIFEQRIVRGDEP
                     IATGKVRVGVIGARSFVPTPLPDDLYETIKAFMAEEA"
     gene            11485..12156
                     /gene="tolQ"
                     /locus_tag="ACZPB6_02330"
     CDS             11485..12156
                     /gene="tolQ"
                     /locus_tag="ACZPB6_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIR1183350.1"
                     /translation="MQPTADLSILTLVANASLVVKCVLAILVFLSLASWTVIFQKYFT
                     ISRAARQVEEFEARFWSGTELNQLLETAKRNHDTAGIEERIFSAGMTEYLKQSRQDPN
                     ALNSVSRAMRAVYNREMDHLERSLPLLASIGSTSPYIGLFGTVWGIMNAFTGLSTLDN
                     VSLAVVAPGIAEALVATAIGLFAAIPAVAAYNYYNNRVGRLTVRLEGFSEEFLNILQR
                     QHARS"
     gene            12160..12591
                     /locus_tag="ACZPB6_02335"
     CDS             12160..12591
                     /locus_tag="ACZPB6_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HIR1183351.1"
                     /translation="MSLRGNTKRRGMMAEMNVVPYIDVMLVLVVILMVAAPFVNPSVV
                     NLPSVNKASKAPEQVIEVIVYPDSRLSIRQGKDMKPVDIPGLVAAVKTAQGEKSNIPV
                     VIAADKDVRYEDVVNVLKLLQSADVPRVGLALRIERSAEAR"
     gene            12616..13818
                     /gene="tolA"
                     /locus_tag="ACZPB6_02340"
     CDS             12616..13818
                     /gene="tolA"
                     /locus_tag="ACZPB6_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HIR1183352.1"
                     /translation="MNDRETNDRFRKEDRRDMVPAAVLSGCVHVILFVGLFSVFQWTT
                     DSETVYAELWAPEPVSGGNDPKGVAVKPPEAPEPKPNEIDEQRESQEAQEAAEREAAA
                     QQARMQEEAAQREAQRQEALAAAERERVEAEKAAEEAREAEEARRAEEARVAAERAEA
                     ERIEKARQDAIEAERRAEEKRAQQEAERLAQEKLAQERKAAEEARLQAEARRQEEARL
                     AEEKRLAEAKRAEEARIAEEKRQAEIQRVAAEKKAAEERRERERIRQAIRQQELARLN
                     AKIDPDSQRSGTPSGDKRNQRQNLTGSALASYNARIIACVRPNISIDVPSSTRRGQFI
                     AEFVVRPMPNGELAGAPVYAKKSGWDAYDSAVRNAILKCKPFPVPEKGYNIPKEVRLR
                     FDPVDDRH"
     gene            complement(13920..>14786)
                     /gene="proS"
                     /locus_tag="ACZPB6_02345"
     CDS             complement(13920..>14786)
                     /gene="proS"
                     /locus_tag="ACZPB6_02345"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR1183353.1"
                     /translation="PLPAKIVLILLRADHDLNEVKAGHLPELKDGFRFATDAEILENF
                     GSKPGYLGPVGIKEDVAVYADLTVANMSDFCCGANEEGYHYTGVNFGRDLPEPKVADL
                     RNVVEGDASPDGKGMLRLQRGIEVGHVFYLGTKYSEALNATYLDENGQPKVLEMGCYG
                     IGVTRLAGAAIEQSHDERGIIWPDSIAPFEVVICPMNYSKSEAVREAADRLYEELKAQ
                     GVDVILDDRDMRPGVMFADWELIGVPHRVVIGDRGLKNGEVEYANRRNLAEKVMVKTE
                     EAVEFVTKQIKR"
BASE COUNT         2871 a         5076 c         4395 g         2444 t
ORIGIN      
        1 gccaagcagg ccatgcgtga agcgggcgtc ccctgcgttc ccggctccga cggtgcgctc
       61 cccgacgatc ctcaggaaat cctccgcatc gcccgccaga tcggctatcc ggtcatcatc
      121 aaggcggcgg gcggcggcgg tggccgcggc atgcgcgtcg tgcgcacgga ggccgcgctg
      181 atcacctcgg tcaacatgac ccgcgaagag gcccgcgtcg ccttcgggaa ccctaccgtc
      241 tacatggaga agttcctcga gaacccgcgc cacatcgaaa ttcaggttct ctccgacaac
      301 ttccagaacg ccgtctatct cggcgagcgc gactgctcca tgcagcgccg caaccagaag
      361 gtgcttgaag aggctcccgc cttcggcatt ccccgcaagc tcatcgaccg cctcggcaac
      421 cgttgcgtgg aggcctgccg ccgcatcggc taccgcggtg ccggcacgtt cgagttcctc
      481 tatgaaaacg gcgagttcta cttcatcgag atgaacacgc gcgttcaggt cgaacacccc
      541 gtcaccgaaa tgatcacggg cgtcgacatc gtgcgcgaac agatccgcat cgccgcaggc
      601 gagcgtctct cggtccgcca gcgcgacatc acgatgcgcg gccacgccat cgagtgccgc
      661 atcaacgccg aggacccgtt caccttcgtg ccgagtcccg gccgcgtcac cgcctggcac
      721 ctgcccggcg gccccggcat ccgcatcgac acgcacgtct tctccggcta cacggttccg
      781 ccctactacg acagcatgat cggcaagatc atcgcccacg gcgatacccg cgagcaggcg
      841 atcgcgcgca tgcgcgtcgc gctctccgaa cttgccgtcg agggcatcaa gaccaacaca
      901 aagcttcatc gcgaactcct cgccgacgaa cgcttctttc agggcggcac gagcatccac
      961 tatctcgaag agaagctccg gtcccgcgcg gaagcccaca agtaacctag ttttttcagc
     1021 tgcgggagtt gacctgactc ccgcattctt tttatccgtt gaggatttat gcgccagatt
     1081 aagattcttg ccgacgaacg cagcgccgaa gcgctttccg acatgctgat ggacgcgggc
     1141 gcgctctccg ccaccatcga ggacgccgac gccgaatcga ccgacgaaaa gccgctctac
     1201 ggcgaaccgg gactcgagcc cgacaactgc gcctggccgc gctccatcgt ctccattctt
     1261 gccgacgacg acttcgacct cgagacatcg cttgccatcg ccgtcaaggc cctcaagtgc
     1321 gaaaagcccg agcttctcgg caccgaggag gtcgaggacg ccgactgggt ccgcatcacg
     1381 caggcgcagt tccagccgac tcaggtctcc gaccgtctct ggatcgtgcc gacctggcac
     1441 gaccttccca acgaggacgc catcaacgtg cgcctcgatc cgggcgtcgc cttcggcaca
     1501 ggctcccatc cgaccaccca cctctgcctg cagtggcttg acgccaacgt caaggcgacg
     1561 gaccgtgtcc tcgactacgg ctgcggcacg ggcatcctcg ccattgccgc caaactcgtc
     1621 ggcgcggccg aggtcgccgg cacggacatc gacccgcagg ccgtcgaggc cgcaggcgac
     1681 aacgcgctca tgaaccacgt ggaagccaag ttctaccttc ccgacgacat gcccgaaggc
     1741 acctacgaca tcgtcgtcgc caacatcctc gccaatccgc tcaagcttct cgcccccgct
     1801 ctgctcggcc gcgtgaagca gggcggcgcg ctcgtgctct ccggcgtgct tgccaagcag
     1861 gccgaagaag tgatcgccgc ctatcaggcc atcgaccctg ccgtcaagct ctccgtctgg
     1921 cgtcaggaag aggactgggt ctgcatcgcc ggacgccgcg cgtaaacgtc aagactgcat
     1981 ctgggagact tctccatggc caaagttctt cgctgtcccg cctgcggggc cctgtggcga
     2041 gtcaaggacg acttcgccgc cgacatgctc cactgcacgg aatgtcaggc atgcttttcc
     2101 gccgacaaga cggaaagcct cagcgtgcca gacgaaaagc tcgacgcccg tcttcagcag
     2161 gccgcccgcc gtcaggccgt catgcaggag gaggctcgcg agggcgaggc cgtcatgagc
     2221 cgcatcgccg attcgctcac ggacttcgac gcaaggaccg aagctcccga ggcccctccg
     2281 gccgccaagc ccgcaccccg tgcgcccgag tccgccgaac ggtcccatgg cgtgctctgg
     2341 acgctcgtca cgcttcttgc cctcgtcgtt ctgtccgccg ccggccttct tctcggccac
     2401 aagaccgtac tcggcacgat gccgcagctg cgccccgtct atgaagaggt ctgcatgaag
     2461 gcaccgtgcc ccggcttcgt ctggcagaac gcggccgcct tcaaggtgac cgcagagatc
     2521 gagcgtccgc tcgagacggc cgacgacgcc gaccgtgaag tcgcccgccg cctgcccgtc
     2581 gtgacggccc gcctctccaa cacctcggaa ctgcctcagg cactgccgat tctggagatg
     2641 aagctgcttg acgccgcagg cgaaacactc gctcaacggg tgctcgagcc tgccgactac
     2701 ggcttcggcg gccgcgacgc cgttgcgccg ggtgccgacg ttgccgtccg cctgacggtc
     2761 acgacgccgc ttccctacga tgcgtcaagt gccgtcgtga aggccgtctc cgcccttcaa
     2821 taaccttcag accaaggcgc tccgccgtac ggcggagcgc cttttgcctt tttgcaaacc
     2881 catgtccgtc atcatttccg gctccatcgc ctacgacacg gtcttcacgc acgagggacg
     2941 ctttgccgac acgctcaggg cagaggcgct cgaccgactc aacgtcacgc ttcaggcgga
     3001 ctccatgagg cgcacctacg gcggctgcgc cgcaaacatc gcctacagtc tgaagcagct
     3061 tggcggagat ccccttgtct ggaccgccgt cggcagggat gcggacgact atctccggca
     3121 tcttgaacgt cagggcattc gcacggacgg cgtgaccgtc atgccgaacg aatggacggc
     3181 gcagtgcatg atcaccaccg acagggacgg caatcagctg gccaccttca gtcccggcgc
     3241 catggcgcac gcagatcgcc ttccctggcc tgacgacacg ggcatagtct gcggaatcct
     3301 cgcccccagc gtgcgaagca cgcttctggc acacgccaag gcctttgtct cgcacggcat
     3361 ccccttcatc ttcgatccgg gccagacgac gcccctcttt tctggcgacg agcttctcgc
     3421 cctgacaaga gcggcggcct gcgttgcatt ctccgactac gaggccctca tgatcgagga
     3481 gcacacgagt ctctctcccg cagaactctc ccgcctcacg ggcgcggtct tctgcaccca
     3541 tggcagccaa gggagctccg tctggcagaa gggcgaggaa accgtcatcc ctgcgccgaa
     3601 ggtcgaggca gccgaccctg tcggcgcagg cgacgcctac cgcggcggcc ttctgtgggg
     3661 catggaaagg gggctcgacg ccgtcagctg cgccattgcc ggcaccgtca tgggagccgc
     3721 aaaggcaacg gccgcgggac cgagctaccg catcaacgcc gatctggtgg aaaaggcagt
     3781 taggaaaggc atgcctttcg actgcaggtc ataaggaaaa aggcgctcca agcctaacgg
     3841 cggagcgcct tttcactgtc tgaagcccgt cgggcgtcaa agcggaatga agccgtggga
     3901 aatcggcgtc acgagcatca gcaggacatt ggccagaatg atcacgacaa tgggcgacag
     3961 atcgaagcga ccgaacatcg gaatgatctt gcgcacggga cgaacgaacg gatccacaag
     4021 cgtgccgagc gcgtaggtca tcgggctctg cggattcagc caggtcatca ccacccagat
     4081 gaagacgccc caggacatca tttcgagcgc ccagcggagc acggtggcca gcggggcgat
     4141 caccagaccg atcggcgtat agggcatccc cgtcagcaca cgctgcatga gcaccatgat
     4201 gacggcggcg acaagcgcag ccacgagact gggccagtcg taggaaccgc tcctcggaat
     4261 gagtttcgag agcggctcca caagccagtc cgtcgcgcgt cgcgcaagct gcacgatcgg
     4321 atgacgcggg gaaaaggccc acacgtagac ccaggcgcgc aacagaagca gcacgccgag
     4381 aatcgacatg acggacgtga agagaaactg aatgagtcca gtgagaaggg tgggcatggc
     4441 gacggagacc tgaattttga attcagtcga ctatatcaga ctatcccgac ctcagggcat
     4501 gacctgaggt tcggttacga tcctgacgcg ccccggaacc atttccctga cgcgatccca
     4561 gtcaaaggag gggccggggt cctgcttgcg gccgggcgcg atgtgctcgt gccccgtcac
     4621 ggcttccacg ggcagcactc ttgtcagcgt ccccagaagt tctccgagcg ccaggtactg
     4681 agcgtcttca tagggcgtct cgcccgtgcc ctcgagctcg attccgacgg aaaagtcgtt
     4741 gcaccccgtg tgcccctcga aggacgagac gccagcatgc caggccctgt ccagaacgct
     4801 cacatactgt ctgacttctc ccgtgcggcg cacgaagaaa tgggaggaga ccctcaggcc
     4861 cctcagggac tcgtattcgg ggcggttcat ggcgtcaagg gtccccatga agagggcgtc
     4921 gacgtcctcc ccgccgaagc gtcctgcagg cagagagatg aagtgaagga ccgccagcga
     4981 aacgagcgcc ccttcgggac gctcgttgaa gtgcggagag agccggcgca cgacgccggg
     5041 cagccacccg tcgaaggcgc ggtcggggga tgtcattgcc cgtcccgcgc cgcatcgcgg
     5101 cagcgcttcg agcagtagac gcgctccttg ctgagcacgg cctcgcgttt gggaaagtaa
     5161 aggccgcagt ggtcgcactt gaccatcacc tcgcctgaag gctcgcgaga ctttccctga
     5221 gcctcgcgct ccttggcacg caggcgcttg agttcacggc gctcctcgtt gtcaagactg
     5281 ttgcgcctgc gactgagcgt ccaggcaacc cacatggcga cagccagcgc cacgaaaaag
     5341 aggatgcgtc ccatgaatcc tcctcagcgg accagttcct gaatgaagct gtagccgaag
     5401 taggccacga cgaagacgac gaagcctgcc cagaaccagt tgagggcggt gcgggcgcgc
     5461 cagcccgcaa cgcggcggcc tgcaagcagg atgccgaaga acacccacgc aagccacgtg
     5521 agaatcgtct tgtggtcaaa gaggaagtag acgcccttgg cctcctgagt ggcaagtgcg
     5581 ccgacgatca gcgtgagcgt gatgaagagg aagcccacga ccacgattcg aaagaagatg
     5641 cgctccatga cgaccagagc gggcatggag tcgaggaact tcgacgtctc gccgaccgcc
     5701 gggctcttca ggcggcggtt gatcagcgcc atgagtacgg cgtggacgaa ggcgatcatg
     5761 aggaaggcgt aggccgcgat ggcaagcagc agatgccagc ggaagatcgt cgtccactcg
     5821 acggagggga tcacctggcc ggtgaacagg agcgggctca gcgtgccgac ggcggcagcg
     5881 atgagcacga tgccgaactg gccgtgcaga cgatgaatcc acgtctcgat cagaaggatg
     5941 atcaccgcaa aaaagaacat ttcggacatc gcgaagccga atccgaagtt gactgcacct
     6001 tcgccgaaca tttcgccgtg aagggcgtag ccctgcatca ccagaccggc aagcacgggc
     6061 cagcggatga gggaggaggc tcccgagccc tcgcccttca tcgagccgat gatggccatc
     6121 cctgagccca gataaaggat cgcagcaatg atggtgcaga tggtaagaat ggtcattctg
     6181 tgttctcttg ttttttgact tgttcgatcg aggcgcgggc gttggccggc gtgctcgagg
     6241 aggctggaga tcgatgcaaa actcggccgt tcccactaaa atgagaagtt tgccgaagga
     6301 cggtgaaagc cgctccggct tgaagggccg gaagcgctcc cgacgcctgc ggcggacccg
     6361 aacagcatcc gaagacacgc cttgcaaaca tgattctagc cgcaacgggc ttagatatcc
     6421 ctgaaatcgc gtctcggact ccaattttca gaagcgctcc cccaagcccg ctctttcggg
     6481 agccgcgacg gccggcgcta cgcgcccgcc gtcctcagga caggcccttc aggcgcctgt
     6541 ccgtcaaccc caggaatcca taaaccatgc tcgactcatt aagccagcgc ctttccaaaa
     6601 tcgtcaagac catgcgcggt caagcgcgtc tgacggaaga aaacacgaag gacatgctgc
     6661 gtgaggttcg cattgcgctt ctcgaggccg acgtagccct gcccgtcgtg cgtgaagtgc
     6721 tcgcccgcat caaggaaaag gccatgggcg aagaggttgt cggtaacctc aatcccggcc
     6781 aggccttggt gggcgtcgtg cagcgcgagc tcaccgccat catcggcggc gacgtgcccg
     6841 ccgccgaacg cgacatcaat cttcacaccc agcctcccgc tgtcatcctt ctcgccggcc
     6901 ttcagggcgc gggcaagacg acgagcgctg gcaagctggc caagtacctc atcgaaacga
     6961 agcgcaagaa ggttctcacg gtgtccgccg acgtttatcg tccggccgcc atcgaacagc
     7021 tcaagacggt gaccgaacag gcgggcgccg aatggttccc gagcaccgtc ggccagaagc
     7081 cgctcgagat tgccgctgcc gccatcgacc atgcccgacg caagttcttc gacgtgctca
     7141 tcgtcgacac ggccggccgt ctggccatcg acgaggcgat gatgcaggag gttgccgagc
     7201 tcaacacctt cctcaagccc gttgaaacgc tcttcgtgat cgacgccatg ctcggccagg
     7261 acgccgtcaa cacggccaag gccttcaacg agcgcctccc gctcaccggc gtggtcctca
     7321 ccaagatcga cggcgacagc cgcggcggtg cggcgctctc cgtgcgtcac gtcaccggca
     7381 agccgattaa gttcgtcggc accgccgaga agctcacggg cttcgacgcc ttcgatcccg
     7441 aggcgatggc ctcccgcatt ctcggcatgg gcgacatcgt cggcctcgtc aaggacgttc
     7501 agaagtcgat caacatcgcc gaagccgaaa agcttgcgca gaagatcagt cagggcgaca
     7561 agttcgacct caacgacttc cgcgcccagc tcgcccagat gaacaacatg gagagcttct
     7621 cctccattct cgagaagctc cccgcgcaat tccaggaggc cgcctccaag gtcaacgacg
     7681 aagaggccaa gaagtccgtc cgccgcacga tgggcatcat cgacagcatg acgcccttcg
     7741 agcgccgcaa gcccgacctc atcaaggcca gccgcaagaa gcgcatcgcc gccggtgcgg
     7801 gcgtccccgt gcaggaggtc aaccgtcttc tcaagcagtt cgagcagacg caggacatga
     7861 tgaagcgcat gaagcgtggc ggtctcggca agatgatgcg cgcgctcggc cagttcggcc
     7921 gcggcggctt ccccggcatg ggcagcttcg gccgctgagc ttcacgatgc gcatcctcgg
     7981 cctcgacccc ggtctcaacc gcacgggctg gggcgtcatc gacgccgcca gcggacggtt
     8041 cgtccgcgtc gacagcggca tgatccgcgt ccccgagggc gagctctcgg ccagactcgg
     8101 gcacatcgta cgcgagcttt cgaaggtgat cgaacgaacg aaacccgacc tcgcctccgc
     8161 cgagcgggtc ttcgtcaacg tgaacccgca gagcacgctg cgcctcggcc aagccagagg
     8221 cgcggcgctc gtcgccgctg accttgccgg cctgcccgtc gaggagttca cgccaagcga
     8281 aatcaaggag gccgtttgcg ccaccggacg cgccgacaaa cccatggtgc agcgcatgat
     8341 cggcatgctg ctcggcatcg aaggcgacat tcaggccgac gaggccgacg cgctcgcctg
     8401 cgcggtatgc tgcgccaact cgattcgcat gcgcgccttt gaaaaggcgg gcagcacgac
     8461 tcgcacctat gcgacgcccc gtcgcggccg cagcgccgca ggtgcgcgca gcgcctggac
     8521 ggcgcttttc acaaaggaag ggaaaacatc atgatcggac ggctgcacgg caagctcatt
     8581 gaaaagaccc cgcctcaggt cctcgttgac gtcggcggcg tcggctacga agtcgacgtt
     8641 ccgatgtcga ccttctgcaa cctgcccgcc gaaggctccg agatcacgct cctcacccac
     8701 ttcatcgtgc gcgaggatgc ccagcttctc tacggctttg caaccgcggc ggaacgccag
     8761 accttcaggg ctctcatccg catttcaggc gtcggcccgc gcattgcgct cgccgtgctt
     8821 tcaggcatga gcactcagga tctcgcagac gccgtcgagc agggcaacgc cacgcttctc
     8881 acccgcgtgc caggcatcgg caagaaaacg gccgaccgtc tggtcctcga gctcaagggc
     8941 aagctcgccg gcaacgcctt cgcgcctgcg ggcggagccg catccgcagc ccaggccgac
     9001 attctgagcg ccctgatggc gctcggctat tccgaacgcg aggctcaggc gagcgtgcgc
     9061 gcgctccccg ccgaagtgac ggtgagcgaa ggcatccgtc tcgccctcaa ggcgcttgca
     9121 cgctgaacga ggtcatgaat gcttgaggaa gaacgcatca tcgacggccg cacggccagt
     9181 cccaacgaag agaacgtcga ccgcgcgctg cgcccgtcgc ttctcaacga atatgtcggg
     9241 caggaggcca tccgcgagca gctccagatc ttcattgccg ccgccaagcg ccgcggtgag
     9301 ccgctcgacc accttctgct cttcggccct cccggcctgg gcaagacgac gctagcccac
     9361 atcgtcgcgc acgaaatggg cgtcaatctc cgccagacct cggggcccgt tctcgagcgt
     9421 cccggcgatc tggcggccat gctcaccaac ctcgagccgc acgacatcct cttcatcgac
     9481 gaaatccacc gtctctcgcc tgtggttgag gaaatcctct accccgcact cgaggactac
     9541 cagatcgaca tcatgatcgg cgagggtcct gccgcgcgtt cgatcaagat cgacctgccg
     9601 cccttcacgc tcatcggcgc cacgacgcgc gcaggctcgc tcacaaatcc cctgcgcgcc
     9661 cgcttcggca tcgtcaacca gctgcagttc tacacgcccg aggagctctc ccgcatcgtg
     9721 cgccgctcgg cgggcctgct caacgtcggc atcgacgaga cgggcgcgtt cgaaatcgcc
     9781 cgtcgcagcc gcggcacgcc ccgcgtcgcc aacagacttc tgaggcgcgt gcgagattat
     9841 gccgaagtga agcacgacgg ccgcatcacg aaggaagtgg ccgcggccgc cctcaagatg
     9901 ctcaacgtcg accccgaagg cctcgacatg atcgacactc actttctcag gacgatcatc
     9961 gagaagttct cgggcggtcc cgtcggcatc gagaacctcg ccgcctccgt cggcgaagac
    10021 cgcgatacgc tcgaggacgt cgtggagccc tacctcattc agcagggctt cgtgcagcgc
    10081 acgccgcgcg gccgagtcgc cacgccgctc gcctggcagc acttccgcct ggcgccgccc
    10141 acgcccgtcg cgcgtgatct tttcggctcc tactgacctc cgccatgccc ttttccccga
    10201 ttctctccgt tctcgtctgc ctcacctcag ccgccgtgtt cgccgcctgc gtgctcttca
    10261 tccgaagcgc acgccaccgc gccatccttc atgagctact cgctcacccg gcccgtcagg
    10321 cgctcgccgt ctttgcggcc gccacgctcg gagcagccct tctgagcgcc atgcccatcg
    10381 atcgggacgg caggaccgac actctgctgc aggccaccat cgaggccacg ggtgcaggtc
    10441 ttcccgaaat gagcgccagc gccccctttg ccgtcgtcga cgaccacggc aggcagctca
    10501 tgcacgcagg cgaaaggctc tcctccgaga cgcctccgct catccacatc atcaagtaca
    10561 cgctcgtcgc cctgatcgca ggcggcgtca cgagctttgc atcgattctc ttcacggccg
    10621 ccatcatcgg cgcgcgcaaa ggccaaaatc tcgcaacggg catcacaatg ctctcgagct
    10681 tctactggcg tcccgggatg ctcacgggga ccgcgctctg gatgattctc acgtggctcc
    10741 tgctgctctg gcccgactgg cacgtgctgg gcaccacggc tgcgggcacg gacgtcatgg
    10801 cgatggtcgc tggacttctt ccttggggca cctgcgcggc cttctggtac cttgtcttcg
    10861 cctactggcg caacatcagg gcctaagcgg acgcataagc atccgtttgc aaaatcgacc
    10921 tccgctcgtc tcccgaacac gccgcgagtc cctatactag tttctttccg gtgcctgacg
    10981 gcaccccgat gcacaaccaa cactcgtcat gaaccacatc agatttcccg tccgcgtcta
    11041 ctgggaggac actgatgccg gcggcatcgt ctaccatgcc aactacctga agttcatgga
    11101 gcgcgcccgc agcgacatgc ttcgacggat cggctttggt cagaacgatc agcgcggccg
    11161 tgaggacggc gttctcttcg tcgtcgcctc actcaacatc gcctatcacc gcgccgcggt
    11221 gcttgacgac gacctggagg tgatcacgag cattgagaag ctcgggcgcg cctcgctcat
    11281 cttcgagcaa cgcatcgtgc gcggcgacga gcccatcgcc acgggcaagg ttcgtgtcgg
    11341 cgtcatcggc gcaaggagct tcgttccgac tcccctgccc gacgacctct acgaaacgat
    11401 caaagccttc atggcagagg aagcctgagc gcccctcgcc gaacactctt tcattcaacg
    11461 ccattcaaaa agcggttatc taaaatgcag cccactgccg atttgtcgat tctgaccctt
    11521 gtcgccaacg ccagcctcgt ggtgaagtgc gtattggcca ttctcgtctt cctctcgctc
    11581 gctagctgga cggtcatctt ccagaaatac ttcacaatct cccgcgccgc gcgacaggtc
    11641 gaggagttcg aggcccgctt ctggagcggc acggagctca atcagcttct tgaaaccgcc
    11701 aagcgcaatc acgacaccgc aggcatcgaa gagcgcatct tcagcgcggg catgaccgaa
    11761 tacctcaagc agagccgtca ggatccgaac gccctcaaca gcgtctcccg cgccatgcgt
    11821 gccgtctaca atcgcgagat ggaccatctc gagcgcagcc tgcccctgct cgcctcgatc
    11881 ggctccacct ctccctacat cggcctcttc ggcacggtct ggggcatcat gaacgccttc
    11941 acgggcctct ccaccctcga caacgtgagc ctcgccgtcg ttgctcccgg catcgccgaa
    12001 gcactggtcg ccaccgccat cggcctcttc gccgccatcc ctgcggtcgc cgcctacaac
    12061 tactacaaca accgcgtcgg ccgcctcacg gtccgcctcg aaggcttcag cgaagagttc
    12121 ctcaacattc ttcagcgcca gcacgcccgg agttaagcca tgtccctgcg tggaaatacc
    12181 aagcgccgcg gcatgatggc cgagatgaac gtcgtgccgt acatcgacgt catgctcgtg
    12241 ctcgtcgtga tcctgatggt cgccgcgccc ttcgtgaatc cgagcgtcgt caacctgccg
    12301 tcggtcaaca aggcctccaa ggcccccgag caggtgatcg aggtcatcgt ctatcccgac
    12361 agccgccttt cgatccgtca gggcaaggac atgaagcctg tcgacattcc cggcctcgtt
    12421 gccgccgtca agacggctca gggcgagaaa agcaacattc ccgtggtgat tgccgccgac
    12481 aaggacgtcc gctacgagga cgtcgtcaat gtcctcaagc tccttcagag cgccgacgtg
    12541 ccccgagtcg gtctcgccct tcgcatcgag cgaagcgccg aagcccgcta acccattacg
    12601 caacccaatt tcgtcatgaa cgacagagag accaacgacc gcttccgaaa agaggaccgc
    12661 agggacatgg tgcccgccgc cgtcctctcg ggctgcgtgc acgtcatcct tttcgtcgga
    12721 ctcttctccg ttttccagtg gacgaccgat tccgaaaccg tctacgcgga gctctgggct
    12781 cccgagccgg tctcgggcgg caacgacccc aagggcgtcg ccgtcaagcc gcccgaagcg
    12841 cctgaaccca agcccaacga gatcgacgag caacgcgagt ctcaggaggc tcaggaagcc
    12901 gccgaacgcg aggctgccgc tcagcaggcc cgcatgcagg aggaggcggc gcagcgtgag
    12961 gcacagcgtc aggaggcgct tgccgccgcc gagcgtgaac gtgtcgaggc cgagaaggcc
    13021 gccgaggagg cgcgtgaggc tgaagaggcc cgccgcgcgg aggaagctcg cgtcgcagcc
    13081 gagcgtgccg aggccgaacg catcgagaag gcccgtcagg acgccattga ggcggagcga
    13141 cgtgccgaag agaagcgcgc ccagcaggaa gccgagcgtc ttgctcagga aaagcttgct
    13201 caggagcgca aggccgcaga ggaagccaga cttcaggctg aagcccgtcg tcaggaagag
    13261 gctcgcctcg cagaggaaaa gcgcctggcc gaagccaagc gcgctgaaga ggcgcgaatt
    13321 gccgaagaaa agcgtcaggc tgaaatccag cgtgtcgccg ccgagaagaa ggctgcggaa
    13381 gagcgtcgtg aacgcgagcg aatccgccag gccatccgac agcaggaact cgcacgactc
    13441 aacgccaaga tcgatccgga cagccagcgt tcgggcacgc cctcgggcga caagcgcaac
    13501 cagagacaga acctcacggg gtcggctctg gccagctaca acgcccgcat catcgcctgc
    13561 gtgcgtccga acatttcgat cgacgtgccc agctccaccc gccgcggaca gttcatcgcc
    13621 gaattcgtcg tcaggccgat gcccaacggc gaacttgccg gcgctcccgt ctacgcgaag
    13681 aagtcaggct gggatgccta cgactcggcc gtacgcaacg ccatcctgaa gtgcaagccc
    13741 ttccccgttc cggaaaaggg ctacaacatt ccgaaggaag tgcgcctgcg cttcgacccc
    13801 gtcgacgaca ggcactgagc gccgtcatcg tccgcacgca aaaagaggag gtctccggac
    13861 ctcctctttt tttgtcattc gtctgggtgc tccgcaacgg agcacccgtg cagtcaggct
    13921 cagcgcttga tctgcttcgt cacgaactcg acggcttcct ccgtcttcac catcaccttt
    13981 tcggcaaggt tgcgacggtt ggcgtattcg acctcgccgt tcttgaggcc gcggtcgccg
    14041 atgacgacgc ggtgcggcac gccgatgagc tcccagtcgg cgaacatcac gccgggacgc
    14101 atgtcgcggt cgtcgaggat gacgtcgacg ccctgagcct tgagctcttc atagagacgg
    14161 tcggcggctt cgcgcacggc ttcgctcttc gagtagttca tcgggcagat caccacttcg
    14221 aacggggcga tggagtccgg ccagatgatg ccgcgctcgt cgtgactctg ctcgatggca
    14281 gcgccggcga gacgggtcac gccgatgccg tagcagccca tctcgagaac cttcggctgg
    14341 ccgttttcat cgagataggt ggcgttgagg gcttcggagt acttcgtgcc aaggtagaag
    14401 acgtgaccga cttcaatgcc gcgctgcagg cggagcatgc ccttgccgtc gggagaggcg
    14461 tcgccctcga cgacgttgcg caggtcggca accttgggtt cgggcaggtc gcggccgaag
    14521 ttcacgccgg tgtagtgata gccctcttca ttggcgccgc agcagaagtc ggacatgttg
    14581 gcgacggtca ggtcggcata gacggcaacg tcttccttga tgccgacggg gccgagatag
    14641 ccgggcttgg aaccgaagtt ctcgaggatc tcggcgtcgg tcgcgaagcg gaagccgtcc
    14701 ttgagctcag gcagatgacc ggccttgact tcgttgaggt cgtggtcggc acggagcagg
    14761 atgaggacga tcttggcggg aagcgg
//
LOCUS       DBMDTI010000022        14554 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1301_length_14554_cov_3.180170, whole genome shotgun sequence.
ACCESSION   DBMDTI010000022 DBMDTI010000000
VERSION     DBMDTI010000022.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 14554)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14554)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14554
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1301_length_14554_cov_3.180170"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(72..569)
                     /locus_tag="ACZPB6_02350"
     CDS             complement(72..569)
                     /locus_tag="ACZPB6_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR1183354.1"
                     /translation="MSLEIRPVREEDVEVVCGLIHALARYERLESHCKATSEQLREEL
                     FGPRAVIGCVLAWEVNEATGAERPVGFALYFYNFSTFLTRRGLYLEDLFVVEDARRRG
                     YGKAIIRHLAQKALDEGCGRFDWSVLDWNQPAIDFYEGLGAEVLPDWRICRLSGEALL
                     RAAGR"
     gene            complement(740..837)
                     /gene="ffs"
                     /locus_tag="ACZPB6_02355"
     ncRNA           complement(740..837)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACZPB6_02355"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1141..1464
                     /gene="trxA"
                     /locus_tag="ACZPB6_02360"
     CDS             1141..1464
                     /gene="trxA"
                     /locus_tag="ACZPB6_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR1183355.1"
                     /translation="MSEIIHVTDENFEEVVSQPNVTVLVDFWAPWCGPCRMLAPRLEQ
                     AAETFDGRVVIAKYNCDEASRFAGEYNVRGIPTVIAFKNGQHVGERTGLCDQETLNAF
                     IEAHL"
     gene            1567..2823
                     /gene="rho"
                     /locus_tag="ACZPB6_02365"
     CDS             1567..2823
                     /gene="rho"
                     /locus_tag="ACZPB6_02365"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR1183356.1"
                     /translation="MHLSDLKSLHISQLLDIAYALEIDNAQRMRKQELMFAILKKKAK
                     SGEQIFGDGTLEVLPDGFGFLRSPDTSYLASTDDIYISPSQIRRFNLHTGDSIEGEVR
                     TPKDGERYFALVRVDSVNGLPPESIKHRMLFENLTPLFPNEHLVLERDMRGDENLTGR
                     IIDMIAPIGKGQRALIVAPPKSGKTVLMQHIAHAIIANHPDVTLFVLLIDERPEEVTE
                     MQRSVKAEVVASTFDEPAARHVQVAEMVIEKAKRLAESKRDVVILLDSITRLARAYNT
                     VIPSSGKVLTGGVDANALQRPKRIFGAARNLEEGGSLTIIGTALVDTGSRMDDVIYEE
                     FKGTGNNEIHLERRMAEKRVYPAINLNRSGTRREELLLKPDVLQKVWILRKFLYDMDE
                     LEAMEFLLDKMKATKNNVEFFELMKK"
     gene            complement(2953..3729)
                     /locus_tag="ACZPB6_02370"
     CDS             complement(2953..3729)
                     /locus_tag="ACZPB6_02370"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR1183357.1"
                     /translation="MALQRPVILMRPGEANNRLAGVLLKEGIECWRWPAFRIELPGKE
                     DCDLVQERLDNLDDVEMVILPSPASVAAAAHWVKAWPEHITLATVGEGTAKVIRAAWG
                     DDVKLIYPEGDAEDSGSEALWEILKHRGAPSRVLFLRGQTGREWLPEQLRSIGSDVIT
                     MCIYVRVPLELTPEQRSDILMAAHGPSPIIYITSTDAVDALFHAIRPVSEVRDWVTNG
                     VAVTLHPRIVRRLQEAGFHHIEVTCTDDEVVASHIRANLP"
     gene            complement(3749..4678)
                     /gene="hemC"
                     /locus_tag="ACZPB6_02375"
     CDS             complement(3749..4678)
                     /gene="hemC"
                     /locus_tag="ACZPB6_02375"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR1183358.1"
                     /translation="MSNFERCVIATRESPLAMWQALHIQSVLRARYPSASVELLGMTT
                     RGDQILDRTLSKVGGKGLFVKELEVAMEAGEADLAVHSLKDVPMELPEGFDLVAVSER
                     EDPRDAFVSPKYASLDDMPAGARVGTASLRRELMLRMNYPHLEVLPVRGNVGTRLRKL
                     DAGEYDALVMASAGLKRLELHDRIRSIIPAEVSLPSPGQGALGVEIRSDNAVMHEALA
                     FLNDPHTRACCTAERAVSRALGGSCQVPLAAYGIIEGGELYLRALVGNHKTGEAIRSE
                     ARGPWTEPEALAARVVEDLRRQGADAILKAVLG"
     gene            5014..5301
                     /locus_tag="ACZPB6_02380"
     CDS             5014..5301
                     /locus_tag="ACZPB6_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HIR1183359.1"
                     /translation="MKKGIHPEYREVAFVDLSINQTFIIRSAAQTRETIEIDGVTYPM
                     YKLDTSSASHPFYTGAQTRIVEAGRVEKFRAKFAAKQQAMNAAAEAAKAAK"
     gene            5445..7157
                     /locus_tag="ACZPB6_02385"
     CDS             5445..7157
                     /locus_tag="ACZPB6_02385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR1183360.1"
                     /translation="MLSQRSTPVKMSQEAARKLPRWSLLALLTIFAFSGFWATGLWTL
                     RDALSFGTASAMLDGGASAWLMPMMADSPITEAGPLAGWLTAVIIAFGRLTGLMGDIA
                     AVRFAACLLFALTTAALWYGTWHLARRPEAQPIAFAFGGEASPRDYSRVVADVAVLLF
                     VATFGILTRQHEALPDTTLLTMAALSFYGLTLGIRRPVPGAFTAGLAAGLAVVSSTLF
                     AGCWLLVLALITIQCLKAFSHHRPKRLLITIAGALAGFLPWPLLAFAVDPAQAAAWFG
                     EWLPAQLAHFSLAGSDTYLWFARNALWYLCPIWPFAIWGLYVWRHQIRQTHILLPAAA
                     LMAGIPAVVFSSFQAYDTVFLAFLPMLSVTAAFGLVTVRRGYENVLDWFSLTIFTLGL
                     LVLWAYWFAWLTSFAPKMAESLQMLAPGSAPVFDSGLVLACLVSLVWFVFVGWRLTHR
                     PVVAWRGPWLAAAGITAFSASLVGLYHTALDVNRSYEPVVKAVARNLEAKGMQPGDVV
                     CGTGMNHGLQAVFKHYADLDVLVRARPDECRFTIDRSRSGALLPDSFRRPHTDEAFTV
                     IQNR"
     gene            7170..8579
                     /locus_tag="ACZPB6_02390"
     CDS             7170..8579
                     /locus_tag="ACZPB6_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1183361.1"
                     /translation="MTTLTPPKTSFAALMRLAGPIFVANIAIVGSGTIDTIMAGQLGK
                     NHLAAIAVSIAATISVVMGLAGILQSLSPIAGHHYGARNYRAVGEELEQNFWLGGFLC
                     LFGVPLLMYTDLWISLGKVEGEVARMAAEFLFFTALALPACLFARTFISVNAALSRPR
                     ITMWVSLAMLALKVPLNGIFMYGWLGFPAMGGSGAGVSFCILNYLAFLCYLAIWKRDA
                     FYRTMHAERIFGPRWALMKEQLHVGIPIGLSTFFEVSSFTLMAIFVARFGSEAVSAHQ
                     IVANITSLCYMLPLSIGIASSVLISQCLGARWPAVAYTVLTRTLKVSVASATCIVTIL
                     FFARHWLVTLYSSDTGVCAMAASLLIFGCLYHVFDAMQSVSSFALRGYRVTRLPMIIY
                     GVMLWGVGLGGGYMLAFGNPITGEPMGAYGFWSATAAGLWLTGISLACMAIWVGRQFA
                     RDDNHTPEEIEEAVKVSRA"
     gene            8699..9475
                     /locus_tag="ACZPB6_02395"
     CDS             8699..9475
                     /locus_tag="ACZPB6_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1183362.1"
                     /translation="MSTILFPSPIFGPVHSRRLGVSLGVNLLPADGKLCSFDCIYCEC
                     GLNGQHRPELRMPTREAVREALRAKLESMKAEGVLPDTITFSGNGEPTSHPDFEGIID
                     DTLALRDEYAPKSQVSVLTNATHIVRNGVFRALLRVDNALLKLDTVDEDYIHLVDRPT
                     SRYDLPKLIERMKAFEGRCIIQTMFMKGEWEGRSVDNTGDEYVGPWLDALREIGPRLV
                     TIYTLDRETPGKNLLKAPRERLDEIAARVNALGIPTTVSG"
     gene            complement(9578..10474)
                     /gene="htpX"
                     /locus_tag="ACZPB6_02400"
     CDS             complement(9578..10474)
                     /gene="htpX"
                     /locus_tag="ACZPB6_02400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HIR1183363.1"
                     /translation="MFKRIGLLVLANLAVFIMLSVILTVVQMVFGVNFGTMAGSSLNL
                     SALFIFSMVVGFSGSIISLLMSKQMAKMSMGVQMIDTNNPQPGLEAYLVGVVRHEAER
                     AGIPMPEVGIYEGEPNAFATGASASSAMVAVSTGLLNIMNRDEVEAVLAHEISHVKNG
                     DMVTQTLLQGVMNTFVVFFSRIIGWVVDRQILRNEDDAPGVGYYVTSLVLDICLGFLA
                     GMVVAYFSRWREYHADAGAAEIMQSNQPMINALRRLGGMEAQNELPGAVKGFGISGGI
                     GSLFATHPSIEDRIAALESRRY"
     gene            complement(10715..11878)
                     /locus_tag="ACZPB6_02405"
     CDS             complement(10715..11878)
                     /locus_tag="ACZPB6_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HIR1183364.1"
                     /translation="MESELWYLVLIPVLFAAGWWARGLDAGERERNSRRLPEAYSRGV
                     AMLMSDRPDKAIDPFIEVVRLDPELIELHHVLGTLFRRRGEFERAIKLHNHLVNREDI
                     PEEDRVKALQALGEDYMTAGLFDRAEETYRRLLRNPTEHLDALRALLEIYCIEHEWTS
                     AIDVSRQLEVQAGENLASEIAHYYCELAEEAVRRKDFELADDHVRHALEKCPESPRAG
                     IAAGAVALSLGNAERALRCWNGVMKRTPAYLPLVVGRVADALDALGRRDEAVVLLHKA
                     LADNGGADVMEEAVKRLAAWEGLEKAGESVVEMLARRPSLSAFSTMMTLRQKERPDDE
                     ETKLLATLLQRHGRRFTKYQCSNCGFLASSFSWHCLGCGAWDSFPPRRIEDVK"
     gene            complement(12068..13762)
                     /gene="rpsA"
                     /locus_tag="ACZPB6_02410"
     CDS             complement(12068..13762)
                     /gene="rpsA"
                     /locus_tag="ACZPB6_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR1183365.1"
                     /translation="MTNETNKPESFAELFAESLAHQEMRQGEVITAEVVRVDYNFVVV
                     NAGLKSEAYVPIEEFKDDRGEVTVQPGDFISVAIESVENGYGDTILSRDKAKRLAAWM
                     NLEQALESGELVTGTVTGKVKGGLTVMTNGIRAFLPGSLVDTRPVKDTTPYEGKTFEF
                     KVIKLDRKRNNVVVSRRAVVEANMGEERAKLLETLKEGAIVKGIVKNITDYGAFVDLG
                     GIDGLLHITDLAWRRVRHPSEVVQVGQELEAKVLKFDQDKNRVSLGLKQLGEDPWVGI
                     ARRYPQGTRLFGKVTNITDYGAFVEVEAGIEGLVHVSEMDWTNKNVDPRKVVTLGDEV
                     EVMVLDIDEERRRISLGMKQCKPNPWEDFAQSHKKGDKVSGQIKSITDFGVFIGLPGG
                     IDGLVHLSDLSWNESGEEAVRKYKKGDELEAVVLAIDTERERISLGIKQMGGDPFSNF
                     ASTHDKNTLVKGTVKSVDAKGAVIDLGNDVEGYLRAAEISADRVEDATTRLAAGDEVE
                     ALITNIDRKNRSIQLSIRAKDAAEARDALDRLNADATAASGTTNLGALLKAKLEQR"
     gene            complement(13868..14539)
                     /gene="cmk"
                     /locus_tag="ACZPB6_02415"
     CDS             complement(13868..14539)
                     /gene="cmk"
                     /locus_tag="ACZPB6_02415"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR1183366.1"
                     /translation="MTDCIPVITLDGPAASGKGTIASRVADALGFHYLDSGALYRIAT
                     LDALSKGIDLDDAQALTAAAQGLSPVFEGGRILLDGRDVTTAIRAEEVSSATSRVAVV
                     PGVRAALFALQKNAARLPGLVADGRDMGTVVFPEAPLKVFMTATARVRAERRYCQLLE
                     RGETADLEALTADLEERDRRDSQRATAPLKPAEDARLLDTSCMGIEEVVKKVLEWWDE
                     TKKLS"
BASE COUNT         2606 a         4816 c         4447 g         2685 t
ORIGIN      
        1 ccgaccagac tttttcttaa aagtcaaggc cgcgttccac ggacttccga gatgaacctt
       61 attttagcgg ctcaacgtcc ggccgccctg agaagcgcct cgcccgaaag cctgcagatg
      121 cgccagtctg gcagcacctc ggcgccgagt ccctcgtaga agtcgatggc gggctggttc
      181 cagtcgagca cgctccagtc gaagcggccg cagccctcgt cgagcgcctt ctgcgcaagg
      241 tgccggatga tcgccttgcc gtagccgcgg cggcgcgcgt cctcgacgac gaagagatcc
      301 tcaaggtaga ggcctcgcct cgtgaggaag gtggagaagt tgtagaagta gagcgcgaag
      361 cccacggggc gctcggcgcc cgtcgcctcg ttcacctccc aggcaagcac gcagccgatg
      421 acggcgcgcg ggccgaagag ctcctcgcga agctgctcgg acgtggcctt gcagtgggat
      481 tcgagtcgct cgtagcgggc gagcgcatga atgaggccgc atacgacctc gacgtcctct
      541 tctcggacgg ggcggatttc caaactcatg agggaaagca attcctgcga tagaaaaatg
      601 cgtccgcgcc cgctcgaagg ggcttgggac atgcaggaac attctagagg aaggcggagg
      661 cggggtgaca aaaaaggcgc gattcgcctt gaaccacgcc cgaacgcgtc cgccacaacg
      721 gaggcggaga aaaaatgaaa atggcgagcc ttgtcctcgg cactgctgct gaccagtatg
      781 gctgcttcgt tcccgacctg accaggttcc tggggacagc atgcgagggg acccgccaag
      841 cccgatagtc tacagaaaga agcagatttt tgcaaaatat cctttttccg ccgtccgaag
      901 ccccctgcac gagcggcctc ttggtgctac tataggtaca ctttccggcg cggattccaa
      961 tccgccgccc tcatccctgg cacggattcg ttctgcagtc cttcaagtgc cgctttcccg
     1021 aacacaacat cttgcactca gtcctgcggc atttatgcct gctcccccgt gcggcccaag
     1081 gtcgacggtc acatgacagg cccgcagtcc gtcaggacag tttctcacac cggagtcatc
     1141 atgagcgaaa tcattcacgt caccgacgaa aacttcgaag aagtcgtctc ccagcccaac
     1201 gtcacggtcc tcgtcgactt ctgggctccg tggtgcggtc cctgccgcat gctcgccccg
     1261 cgcctcgaac aggctgccga aacctttgac ggccgcgtcg tgatcgccaa gtacaactgc
     1321 gacgaagcca gccgcttcgc cggcgagtac aacgtgcgcg gcatcccgac cgtgatcgcc
     1381 ttcaagaacg gccagcacgt cggtgaacgc acgggcctgt gcgatcagga aacgctcaac
     1441 gccttcatcg aagctcacct ctaagccgct ttgcggcagg cggagccgca cgcctcatgc
     1501 ggctccatgc atttctccca acacgcccct ccgacgggcc gtcttctcgg gccgtcaaaa
     1561 tccgctatgc atctttccga cctcaagtcc cttcacatct cgcagctgct cgacatcgcc
     1621 tacgcgctcg aaatcgacaa cgcccagcgc atgcgcaagc aggagctcat gttcgccatt
     1681 ctcaagaaga aggccaagag cggcgagcag atcttcggtg acggcacgct cgaagtgctt
     1741 cccgacggct tcggcttcct tcgcagccct gacacctcct atcttgcgag cacggacgac
     1801 atctacatct cgccctccca gatccgccgc ttcaatcttc acaccggcga ctcgatcgaa
     1861 ggcgaggtcc gcacgcccaa ggacggcgaa cgctacttcg cactcgtgcg cgtcgactcc
     1921 gtcaacggcc tgccgcccga gagcatcaag caccgcatgc tgttcgagaa cctgacgccg
     1981 ctcttcccga acgagcatct cgtgctcgag cgtgacatgc gcggcgacga gaacctcacg
     2041 ggccgcatca tcgacatgat cgccccgatc ggcaagggcc agcgcgccct gatcgttgct
     2101 cctccgaagt ccggcaaaac ggtcctgatg cagcacatcg cccacgcgat catcgccaac
     2161 caccccgacg tcactctctt cgtcctcctc atcgacgaac gtcccgagga agtgaccgaa
     2221 atgcagcgct ccgtcaaggc cgaggtcgtc gcctccacct tcgacgaacc cgccgcccgt
     2281 cacgtccagg tggccgaaat ggtcatcgag aaggccaagc gccttgccga aagcaagcgc
     2341 gacgtcgtca tcctcctcga ctccatcacg cgtctcgccc gcgcctacaa caccgtgatt
     2401 ccgtcttcgg gcaaggtgct cacgggcggc gtggacgcca acgccctgca gcgcccgaag
     2461 cgcatcttcg gcgccgcccg caaccttgag gaaggcggct cgctcacgat catcggcacc
     2521 gccctcgtcg acacgggctc ccgcatggac gacgtgatct acgaagaatt caagggcacg
     2581 ggcaacaacg aaatccacct cgagcgccgc atggccgaaa agcgcgtcta cccggccatc
     2641 aacctcaacc gctcgggcac ccgccgcgag gaactcctcc tcaagccgga cgtcctgcag
     2701 aaggtctgga tcctgcgcaa gttcctctac gacatggacg aactcgaggc aatggaattc
     2761 ctgctcgaca agatgaaggc cacgaagaac aacgtcgagt tcttcgagct catgaagaag
     2821 taagtcccgg agggaccgaa aacaagaaag gcaccgtcaa gatggcggtg ccttttcctt
     2881 ttccgactca agcccgtcgg cccgcgccga agttcacgtc aaaatttaag tcgacttatg
     2941 caaaaccttc cgtcagggca ggtttgcgcg gatgtgggag gcgacgacct cgtcgtccgt
     3001 gcaggtgacc tcgatgtggt ggaagcccgc ctcctgaagt cggcggacga tgcgcggatg
     3061 gagcgtgacg gcgacgccgt tcgtcaccca gtcgcgaacc tccgaaacgg ggcggatcgc
     3121 atggaagagc gcgtcgacgg cgtccgtgct cgtgatgtag atgatcggag acggaccgtg
     3181 agcggccatg aggatgtcgc tgcgctgttc gggcgtgagc tcgagcggca cgcggacgta
     3241 gatgcacatc gtgatgacgt ctgagccgat gctccttaac tgctcgggca gccattcgcg
     3301 gccggtctgg ccgcgcagga agagcacgcg cgaaggagcc cccctgtgct tgaggatctc
     3361 ccagagcgcc tcgctgccgg aatcctcggc gtcgccctcg gggtagatga gcttcacgtc
     3421 gtcgccccag gcggcgcgga tcacctttgc ggtgccctcg cctaccgtgg cgagcgtgat
     3481 gtgctcgggc catgccttca cccaatgggc tgcggcggca accgatgcgg ggctcggaag
     3541 aatgaccatc tcgacgtcgt caaggttgtc caggcgctcc tgaacaagat cgcagtcctc
     3601 cttgccgggc agctcgatcc tgaacgccgg ccagcgccag cactcaatcc cctccttgag
     3661 gagcacgccg gcgaggcggt tgttcgcttc gccgggtcgc atcagaatga cgggcctttg
     3721 taaagccata aatccctctt gacctgaatt aaccgagaac agccttcagt atagcgtctg
     3781 caccctgacg gcggaggtcc tcaaccacgc gcgccgcaag cgcttcaggc tccgtccagg
     3841 gaccgcgggc ctcgctgcgg atggcttcgc cggtcttgtg gttgcccacg agggcgcgca
     3901 ggtaaagctc cccgccctcg atgatgccgt aggcggcgag cggaacctgg caggagccgc
     3961 cgagcgcgcg ggagacggcg cgttcggccg tgcagcaggc gcgggtgtgc gggtcattga
     4021 ggaaggcgag agcctcgtgc atgacggcgt tgtcggagcg gatctcgacg ccgagggcgc
     4081 cctggcccgg agacggcagg ctcacttcgg ccggaatgat cgagcggatg cggtcgtgga
     4141 gctcaagacg cttgaggccg gcggaggcca tcacgagggc gtcgtattcg ccggcgtcga
     4201 gcttgcgcag gcgcgtgccg acgttgccgc gaacgggaag cacttcaagg tgcggatagt
     4261 tcatgcggag catgagctcg cggcgaaggc tggcagtgcc cacgcgggcg ccggcgggca
     4321 tgtcgtcaag gctcgcgtac ttcggggaga cgaaggcgtc gcgcggatcc tcgcgttcgc
     4381 tcacggcgac gaggtcgaag ccctcgggaa gctccatcgg aacgtccttg agggagtgca
     4441 cggcgagatc ggcctcgccc gcctccatgg cgacttcaag ctccttcacg aagagtccct
     4501 tgccgcccac cttggagagc gtgcggtcga ggatctggtc gcctcgcgtc gtcatgccga
     4561 ggagctccac gctcgcggac ggatagcggg cgcgcagcac ggactggatg tgaagggcct
     4621 gccacatggc aagggggctt tcgcgggtgg cgatgacgca gcgttcgaaa ttggacatga
     4681 tgatgaatct gaatgtctgt gaatgtccgt cgtccagacg ttggcggacg gctctaataa
     4741 agggaaaacc gaaaagcggc gggcgccttc cggtcatttt cctgattgta acagtcgaac
     4801 tttgctcgac gcttaacaag atccgccgtt cgtcccgcac gggactcgaa aacctctccc
     4861 gcacgcttgc acgcgacggc gggacgtgac ataatttcgt cctttcccag ttggcacgtg
     4921 gacaggcatg cctgtcgtaa gacccgacga attcgggcgg actcgcaagg ccgtcagggc
     4981 gaccgacaac caaaactagg aattacctta aaaatgaaga aaggcattca tcccgaatac
     5041 cgcgaagtcg ctttcgtcga cctctccatc aaccagacgt tcatcatccg ttccgccgct
     5101 cagacgcgtg aaacgatcga aatcgacggc gtgacctacc cgatgtacaa gcttgatacg
     5161 tcctccgcca gccacccgtt ctacacgggc gctcagacgc gcatcgtcga agccggccgc
     5221 gttgaaaagt tccgcgccaa gttcgccgcc aagcagcagg ccatgaacgc cgccgccgaa
     5281 gccgccaagg ccgccaaata atattgccgg ttcaggcccg tgcggcccgt ccgcacgtcg
     5341 cttccagaaa gggcagtctc cgattcgtcg gactgccctt ttcatttgcc ccgaaaaaac
     5401 ggacaatgtc cgcaatgtcc gaaacgtcaa cttaggagcc ctccgtgctc agccagcgat
     5461 ccactcccgt caaaatgtcc caggaagccg cccgcaagct ccctcgctgg agccttctcg
     5521 cgcttctgac catcttcgcc ttcagcggtt tctgggcgac agggctctgg acgcttcgcg
     5581 acgcactgag cttcggcacg gcctccgcca tgcttgacgg cggcgcatcc gcctggctca
     5641 tgcccatgat ggccgactct ccgatcacgg aggcgggtcc gctcgcgggc tggctcaccg
     5701 ccgtcatcat cgccttcggc cgactcacgg gactcatggg cgacatcgcc gcggtgcgct
     5761 tcgccgcgtg ccttctcttc gcgctcacca cggccgccct ttggtacggc acctggcacc
     5821 tcgcccgacg ccctgaggcg cagcccatcg ccttcgcctt cggcggcgag gcgagtcctc
     5881 gcgactacag ccgcgtcgtc gccgacgtcg ccgtactgct cttcgtcgca accttcggca
     5941 tcctcacccg ccaacacgag gcgcttcccg acacgacgct cctcacgatg gccgcactga
     6001 gcttctacgg cctcacgctc ggcatcaggc gccccgttcc cggcgccttc accgcaggtc
     6061 tcgcagcagg tctggccgtc gtctcctcga cgctcttcgc gggctgctgg ctcctcgtac
     6121 tcgccctcat cacgatccag tgcctcaagg ccttctcgca ccatcgcccg aagcgccttc
     6181 tcatcacgat cgccggcgcg ctcgcgggct tcctgccctg gcccctgctc gccttcgccg
     6241 tcgaccccgc gcaggccgcg gcctggttcg gcgaatggct gcccgcccag ctcgcgcact
     6301 tctcgctggc gggctccgac acctatctct ggttcgcccg caacgccctc tggtacctct
     6361 gccccatctg gcccttcgcc atctggggac tctacgtctg gcgccatcag atccgccaga
     6421 cgcacatcct gctgcctgcg gccgcgctca tggcaggcat ccccgccgtg gtcttctcct
     6481 cctttcaggc ctacgacacg gtcttcctcg ccttcctgcc aatgctctcc gtcactgcgg
     6541 ccttcggtct cgtaaccgtc cgccgcggct acgagaacgt gctcgactgg ttctcgctca
     6601 cgatcttcac gctcggcctg ctcgtgctct gggcctactg gttcgcctgg ctcacgagct
     6661 tcgcaccgaa gatggccgag tccctgcaga tgctcgcccc cggcagcgcc cccgtcttcg
     6721 actcgggcct ggtgctcgcc tgcctcgtct ccctcgtctg gttcgtcttc gtcggctggc
     6781 gcctcacgca ccgccccgtc gttgcatggc gcggtccgtg gctcgccgcc gcaggcatca
     6841 ccgccttctc cgcctcgctc gtgggcctct accacacggc gctcgacgtc aaccgctcct
     6901 acgagcccgt cgtcaaggcg gtcgcccgca accttgaggc caagggcatg cagcccggcg
     6961 acgtcgtatg cggcacgggc atgaaccacg gcctgcaggc ggtcttcaaa cactacgcgg
     7021 acctcgacgt cctcgtgcgc gcccgccccg acgagtgccg cttcacgatc gacaggtcca
     7081 gaagcggcgc cctcctgccc gacagcttcc gtcgtcctca caccgatgaa gccttcaccg
     7141 tcatccaaaa ccgctgaact tagcccctca tgacgacact gacgcctccc aagacatcgt
     7201 tcgcagcgct catgcgcctt gcgggcccca ttttcgtcgc caacatcgcc atcgtcggca
     7261 gcggcacgat cgacacgatc atggccggcc agctcggcaa gaaccacctt gccgccatcg
     7321 ccgtctccat cgccgccaca atctccgtgg tgatgggcct tgcaggcatc ctccagagcc
     7381 tctccccgat tgcgggccac cactacggcg cccgcaacta cagggccgtg ggcgaggaac
     7441 tcgagcagaa cttctggctc ggcggcttcc tctgcctctt cggcgttccg ctcctcatgt
     7501 acacggacct ctggatctcg ctcggcaagg tcgagggcga ggtcgcgcgt atggccgccg
     7561 aattcctctt cttcaccgcg ctcgcgctgc ccgcctgcct cttcgcccga accttcatct
     7621 cggtcaacgc ggccctctcg cgcccgcgca tcacgatgtg ggtctcgctt gccatgctcg
     7681 ccctcaaggt gcctctcaac ggcatcttca tgtacggctg gctcggcttc cccgccatgg
     7741 gcggctcggg cgcaggcgtc tcgttctgca tcctcaacta cctcgccttc ctctgctacc
     7801 tcgccatctg gaagcgcgac gccttctacc gcacgatgca cgccgaacgc atcttcggcc
     7861 cgaggtgggc gctgatgaaa gagcagctcc acgtcggcat tccgatcggc ctgagcacct
     7921 tcttcgaggt ctcgagcttc acgctgatgg caatcttcgt cgcacgcttc ggctccgagg
     7981 ccgtttccgc ccaccaaatc gtggccaaca tcacgagcct ctgctacatg ctgcccctct
     8041 cgatcggcat cgcctcgtcc gtactcatct cccagtgcct cggcgcacgt tggccggccg
     8101 tggcctacac ggtgctcacg cgcacgctca aggtctcggt ggcgagcgcg acctgcatcg
     8161 tcaccatcct cttcttcgcg cgccactggc tcgtgacgct ctactcgtcc gacacgggcg
     8221 tgtgcgcgat ggcggcctcg ctcctcatct tcggctgcct ctaccacgtc ttcgacgcca
     8281 tgcagtccgt gagctcgttc gcccttcgag gctaccgcgt cacgcgcctg ccgatgatca
     8341 tctacggcgt catgctctgg ggcgtgggcc tcggcggcgg ctacatgctc gccttcggca
     8401 acccgatcac gggcgagccg atgggcgcct acggcttctg gagcgccacg gccgcaggcc
     8461 tctggctcac gggcatctcg ctcgcctgca tggccatctg ggtgggccgg cagttcgccc
     8521 gcgacgacaa ccacacgccc gaggagatcg aggaggccgt caaggtctcc agagcctgac
     8581 gacggacttc gggtacacgg cactgacaaa ggcgcgggag gcggctcccg cgcctttttt
     8641 cctgcccgca ggccttgcgc tatgattgac gatcaggtcc ccccgtcagg agttcggcat
     8701 gtcaaccatc ctctttccca gcccgatttt cggtcccgtg cacagccgcc gtctaggcgt
     8761 ttcgctcggc gtcaacctgc tgcccgccga cggcaagctc tgcagcttcg actgcatcta
     8821 ctgcgaatgc ggcctcaacg gccagcaccg tcccgagctg aggatgccca cgcgtgaagc
     8881 cgtgcgcgaa gcccttcgcg ccaagctcga gagcatgaag gccgaaggcg tcctgcccga
     8941 cacgatcacc ttctcgggca acggcgagcc cacctcgcac cccgactttg agggcatcat
     9001 cgacgacacg ctcgcgcttc gcgacgagta cgcgcctaaa agccaggtga gcgtcctcac
     9061 caacgccacc cacatcgtca ggaacggcgt cttccgcgcg ctcctcaggg tcgacaacgc
     9121 gctcctcaag ctcgacaccg tcgacgagga ctacattcac ctcgtcgacc gccccacgag
     9181 ccgctacgac ctgccgaagc tcatcgagcg catgaaagcc ttcgaaggcc gctgcatcat
     9241 ccagacgatg ttcatgaagg gcgaatggga gggccgaagc gtcgacaaca cgggggacga
     9301 gtacgtcggc ccctggctcg acgccctcag ggagatcggc cccagactcg tgacgatcta
     9361 cacgctcgac agggagacgc ctggcaagaa cctcctcaag gcgccccgcg agcgtctcga
     9421 cgagatcgcc gcacgcgtga acgcgctcgg catcccgacc accgtgagcg gctgagtcca
     9481 caaagcgcga acaaaaaaac tccgccagac cagaagtcgg cagagttttt ttcattgctc
     9541 gaggagccgt cgcggaggac ggccctcaga agctgcatca gtagcggcgg ctttcaagcg
     9601 cagcgatgcg gtcctcaatg gacggatggg tcgcgaagag cgagccgatg ccgcccgaga
     9661 tgccgaagcc cttgacggcc cccggaagct cgttctgggc ctccatgccg ccaaggcggc
     9721 gcagggcgtt gatcatgggc tggttggact gcatgatctc ggcggcgccg gcgtcggcgt
     9781 ggtattcacg ccagcgggag aagtaggcga cgaccatgcc ggcaaggaag ccaaggcaga
     9841 tgtcgagaac aaggctcgtc acgtagtagc cgacacccgg agcatcgtct tcgttgcgga
     9901 ggatctgacg gtcgaccacc cagccgatga tgcgggagaa gaacacgacg aaggtgttca
     9961 tcacaccctg aagcagggtc tgcgtgacca tgtcgccgtt cttgacgtgg gagatctcgt
    10021 gggcgagcac ggcctcgact tcgtcgcggt tcatgatgtt gagaagcccc gtggagacgg
    10081 ccaccatggc gctcgaggcg gaggcgccgg tcgcgaaggc gttgggctcg ccttcgtaga
    10141 tgccgacttc aggcatcggg atgccggccc tttcggcttc gtggcgcacg acgccgacga
    10201 ggtaggcttc aagaccgggc tggggattgt tcgtgtcgat catctgcacg cccatgctca
    10261 tcttggccat ctgcttgctc atgagcagcg agatgatcga gccgctgaag ccgacgacca
    10321 tcgagaagat gaagagggcg gaaaggttga gggacgagcc cgccatggtg ccgaagttga
    10381 cgccgaacac catctgcacg accgtgagga tcacgctgag catgatgaag acggcgaggt
    10441 tggcgagcac caacagacca atgcgcttaa acatactgtt ccaatttcct ttttttttct
    10501 gcgtggacag aatgagcgat gcatgttcag ggaacggcgg acgttccatc gctcatgcag
    10561 ccgccgttca ttcattgctt aattgtatga agacgcgacg ttcaatatgt ttggcgtcat
    10621 cacgacaatt gtaacaaagg tctcaaaatc acttggagaa aggccttgat gaccgattcc
    10681 cgtgcaggat gcatgcctcg cgcacgcacg aggactactt gacgtcctcg atgcggcgcg
    10741 gcgggaagct gtcccaggcg ccgcagccga ggcagtgcca ggagaaggac gaggcaagga
    10801 agccgcagtt cgagcactgg tacttcgtga agcggcggcc gtggcgctgc aggagcgtgg
    10861 cgaggagctt cgtctcctcg tcgtcggggc gctccttctg acgaagcgtc atcatggtcg
    10921 aaaaggcgga gaggctagga cgcctggcga gcatctcgac gacgctctcg cccgcctttt
    10981 caaggccctc ccaggcggcg aggcgcttga cggcctcctc catcacgtcg gcgccgccgt
    11041 tgtcggcgag cgccttgtgc agaagcacga cggcctcatc cctgcggccg agcgcgtcga
    11101 gcgcatcggc gacgcggccg acgacgagcg gcaggtaggc gggcgtgcgc ttcatgacgc
    11161 cgttccagca gcgcagggca cgttcggcgt tgccgagcga gagcgcgacg gcgccagcgg
    11221 caatgcccgc acgcggagac tcggggcact tttcaagcgc atgacgaacg tggtcgtcgg
    11281 caagctcgaa gtccttgcgg cggacggcct cctcggcgag ctcgcagtag tagtgggcga
    11341 tctcggaagc gagattttca cccgcctgca cctcgagctg gcgcgagacg tcgatggcgg
    11401 aggtccactc gtgctcgatg cagtagatct caaggagcgc gcgcagcgcg tccagatgtt
    11461 cggtggggtt gcgcaggagc ctgcggtagg tctcctcggc gcggtcgaag aggccagcgg
    11521 tcatgtagtc ctcgccaaga gcctggagcg ccttgacgcg gtcctcctcg gggatatcct
    11581 cgcggttgac aaggtggttg tggagcttga tcgcgcgctc gaactcgccg cggcggcgga
    11641 agagcgtgcc gagcacgtgg tggagctcga tgagctcggg gtcgagcctg acgacttcga
    11701 tgaagggatc gatggccttg tcgggacggt cgctcatgag catggccacc ccgcgcgaat
    11761 acgcctcggg gagccgcctg gaattccgct cgcgctcgcc tgcgtcgagc ccacgggccc
    11821 accagcctgc ggcgaagagc acgggaatga ggaccagata ccaaagttcg gattccataa
    11881 aaggcctttt cttgtcgtta gtcaggattc ggtgacattg tggcacgcga tgcgggctcg
    11941 ggacaaatcc cgcgctgtca tcacgaaaaa aggctgccgg cattttcatg ccgcagcctt
    12001 ttttgtgttc ctctggggac cgtcggaccg aagcccttcg atccccgcgg atcagcttcg
    12061 ttcagaatta cctctgttcg agcttggcct tgaggagagc gccgaggttg gtcgtgccgg
    12121 aggcagccgt ggcatcggcg ttgagacgat cgagagcgtc acgggcttca gcagcgtcct
    12181 tcgcacggat ggagagctgg atggagcggt tcttgcggtc gatgttcgtg atgagagctt
    12241 cgacttcgtc gccggcggcg agacgcgtcg tggcgtcttc aacgcggtcg gcggagattt
    12301 cagcagcgcg gaggtagcct tcgacgtcgt tgccgaggtc gatgacggcg cccttggcgt
    12361 caacggactt caccgtaccc ttgacgagcg tgttcttgtc gtgggtggaa gcgaagttgg
    12421 agaacggatc gccgcccatc tgcttgatgc cgagggagat gcgttcacgt tccgtgtcga
    12481 tcgcgagaac gacggcttcg agttcgtcgc ccttcttgta cttgcgaacg gcttcttcac
    12541 cggactcatt ccaggaaagg tcggagaggt ggacgagacc gtcaatgccg cccgggagac
    12601 cgatgaacac accgaagtcg gtgatggact tgatctggcc ggagaccttg tcgcccttct
    12661 tgtgggactg ggcgaagtct tcccacggat tcggcttgca ctgcttcatg ccgagggaga
    12721 tgcggcggcg ttcttcgtcg atgtcgagaa ccatgacttc gacttcatcg ccgagcgtga
    12781 cgaccttacg cggatcaacg ttcttgttcg tccaatccat ttcggagacg tggacgagac
    12841 cttcgatgcc ggcttcgact tcaacgaagg caccgtagtc ggtgatgttc gtgaccttgc
    12901 cgaagaggcg ggtgccctgc gggtagcggc gggcgatgcc gacccacggg tcttcgccga
    12961 gctgcttgag gccgagggag acgcggttct tgtcctggtc gaacttgaga accttggctt
    13021 cgagttcctg accaacctga acgacttcgc tcgggtggcg gacgcgacgc caggcgaggt
    13081 ccgtgatgtg gaggagaccg tcgatgccac cgaggtcgac aaaggcgccg tagtcggtga
    13141 tgttcttgac gatgcccttg acgatggcgc cttccttgag cgtttcgagg agcttggcgc
    13201 gttcttcgcc catgttggct tcaacgacgg cacggcggga aaccacgacg ttgttgcgct
    13261 tgcggtcgag cttgataacc ttgaattcga aggtcttgcc ttcgtacggc gtcgtgtcct
    13321 tgaccggacg ggtgtccacg agggagcccg ggaggaaggc gcggatgccg ttggtcatga
    13381 cggtgagacc gcccttgacc ttgcccgtga ccgtaccggt gacgagttca ccggattcga
    13441 gagcctgttc gaggttcatc caggcggcga ggcgcttggc cttgtcgcgg ctgaggatcg
    13501 tgtcgccgta gccgttttcg acggattcaa tggcgacgga gatgaagtcg cccggctgga
    13561 cggtcacttc accacggtca tccttgaact cttcgatggg cacataggct tcgctcttga
    13621 ggccggcgtt gacgacgacg aagttgtagt cgacgcggac gacttcagcg gtgatgactt
    13681 caccctgacg catttcctgg tgagcgaggc tttcggcaaa aagctcagcg aaggattcgg
    13741 gcttgttggt ttcgttggtc attgtttgaa ataaagatac acacccgcag cagaaggcct
    13801 tgggcggagt tgatgaaaat gcaagacgcc ccttcgggcg acctgcggaa aaaaatgaac
    13861 gcttattcta cgacaacttt ttggtctcgt cccaccactc gaggactttt tttacgactt
    13921 cctcgatgcc catgcaggac gtgtcgagaa ggcgtgcgtc ctcggcgggc ttcagaggcg
    13981 ccgtggccct ctggctgtcg cgacggtcgc gctcctcaag atcggcagta agcgcctcga
    14041 ggtcggccgt ttcgccacgt tcgagcagct ggcagtacct gcgctcggcg cggaccctgg
    14101 ccgttgcggt catgaagacc ttgagcggag cctcggggaa gaccaccgtg cccatgtccc
    14161 tgccgtcggc gacgagaccg ggcaggcggg cggcgttctt ctgcagcgca aagagcgcgg
    14221 ctcgaacgcc gggcaccacg gccacgcggc tcgtggcgga gctcacttcc tcggcacgga
    14281 tggccgtcgt cacgtcgcga ccgtcgagaa ggatgcggcc gccctcgaac acgggggaga
    14341 gcccttgggc cgccgccgtg agcgcctgcg cgtcgtcaag gtcgatcccc ttcgaaaggg
    14401 cgtcgagggt cgcgatgcgg tagagcgcgc cgctgtcgag gtagtggaag ccaagcgcgt
    14461 cggcgactcg ggacgcgatc gtgcccttgc cgctcgcggc ggggccgtca agcgtaataa
    14521 ccggaatgca gtctgtcatg aaatctctcg tctg
//
LOCUS       DBMDTI010000023        14487 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1309_length_14487_cov_2.820242, whole genome shotgun sequence.
ACCESSION   DBMDTI010000023 DBMDTI010000000
VERSION     DBMDTI010000023.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 14487)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14487)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14487
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1309_length_14487_cov_2.820242"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="ACZPB6_02420"
     CDS             <1..244
                     /locus_tag="ACZPB6_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183367.1"
                     /translation="YEVRTNARAIEAVRFQTFENSVPALEASGRANPGSGLTVDNVAA
                     YMVQLLNQTKKRLGDRAVLPAAPEPVPTQAPAARQP"
     gene            complement(345..776)
                     /locus_tag="ACZPB6_02425"
     CDS             complement(345..776)
                     /locus_tag="ACZPB6_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183368.1"
                     /translation="MSNITLPMELMLRATLPTRSEQLLNNLRFLPRELHPQSVNGLRS
                     LGFDANLCADLNSFKADRNVDTPLILALFINEFFKAAGSDYAASQSIAKYLRRRARKI
                     DVEPFGQEVAEIVKKHCKELRSAAIGPFIRDAEPYRVVQLY"
     gene            1251..2066
                     /locus_tag="ACZPB6_02430"
     CDS             1251..2066
                     /locus_tag="ACZPB6_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003817715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1183369.1"
                     /translation="MKFKLFASVCVAAGLAFGLAGCGDDNAPKSGDAKTLTVGASPVP
                     HADILNLIAPQLQKEGITLKVIEFSDYIQPNLSLNDKELDANFFQHKPYLDSFSKDRG
                     LKLTSLVAVHIEPMGVYSHKVKNVADVAEGSEVAIPNDPTNGGRALKVLETAGLIKVR
                     PEAGVTATINDVVDNPKKIKFREIEAAQLPRALDDVALAVINSNYALGAGLNPVKDAL
                     AIESKDSPYANIVAIRTGDDSRAEIQKLKAALTSAEVKKFIETEYKGAVVPAF"
     gene            complement(2145..2516)
                     /locus_tag="ACZPB6_02435"
     CDS             complement(2145..2516)
                     /locus_tag="ACZPB6_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR1183370.1"
                     /translation="MTDKTPAGEVLCPVALTLSLIGGKWKALILWNLLEGTMRYSALR
                     KAVPDATPKMLTQQLRELEADGLVSRTVWPVVPPRVDYALTPTGESIKPILTAMYRWG
                     DAYLAKRNRKSCCSMKPPADE"
     gene            2622..5171
                     /locus_tag="ACZPB6_02440"
     CDS             2622..5171
                     /locus_tag="ACZPB6_02440"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1183371.1"
                     /translation="METRQFNVTGMSCAACSSRVEKAVCALRGVSAAEVNLLTNSLKA
                     TFDEGVLGVEDIERAVEDAGYGASEKGFAGRGRTAAATAPEADAEAAAEAVRVRLIWS
                     FALLAPLMWLSMGPMAGLPVPVMLTGAQGAPMMAFTQFLLTLPIVLLNRRYFTGGLRA
                     LVNRAPNMDSLIAVGSGTALLFGVYVIYRLLAAQAAGDEAALHHFAHSLYFEGAATIV
                     TLISLGKFFEARAKRRTTEAVTALMKLAPPTALVVRDGAEVRIGTDEVASGDIVVVKT
                     GETVPVDGVILEGTALLNESALTGESLPVTRGPGEAVTGATLLAAGAFRMRATRVGDD
                     TVLAGIIRLVEEATASKAPVARLADKVSGIFVPVVMTIALVSGLVWYWIGGDLEFALN
                     AAVSVLVISCPCALGLATPTAVMVGMGRGAGMGVLFKSAEALEVFSGLKTVVFDKTGT
                     LTEGRPVVTKVAAAVPGLETVVMLVAAALEKPSEHPLAQAVLAEAEARGLTAQDVDEF
                     EQIPGGGLRGLMSGTPVAAGNARLMETLGLEVPEALSALALAEASQGATPLFIASAGS
                     VIGIIAVADRVRSESRAAVSALRGRGLRVVMLTGDNRRTAEAVGARLGIASEDIVADV
                     RPAEKASHVKALQADGPCAMVGDGVNDAPALAQADAGVAVGGGTDAARASADVVLMRD
                     SPEGVVGAVDLSRAVMRNIRQNLFWAFAYNVIGIPIAAGVLYPMFGLLLNPMIAAAAM
                     SMSSVSVVTNALRLRFFKAPVLVSDSGTDGASTTGSSMMEKVIHIEGMHCGHCTAAVE
                     KALRALPGVESAVADLEKKEARVKAAAGVSDVMLSAVVTGAGFKVTGIDTL"
     gene            5320..7086
                     /locus_tag="ACZPB6_02445"
     CDS             5320..7086
                     /locus_tag="ACZPB6_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1183372.1"
                     /translation="MRIIKWLRASIVRQCFVVLAVIGLLAVLAAAPALILTERSTGSG
                     GAINVSGSMRMQSYKLALAVADPFSSFEQRREKTMTALDEFGVKLMSPGLLNGVPHDS
                     GNPAYIQYVALKDRFEKGVRPLAEASIDSLEARRRFTEQIPSFVEDVDRFVQTLENGL
                     NERLALLQKILTLILFGAMGVSFVMLLVMQRSIFRPLLAIGSAADAVRKGDFSVRAKV
                     PAPDEIGRLATSFNFMVDELGRLYGNLEHEVEKKTEDLNRRNAGLQFLSRVAQSAAWG
                     AVFKRHSLDDLLEESCVQFGARAVRLVVSTDGTAAVLGESDAWPEDEPETGVVRFVLS
                     NANPSMGTLEAVFDAEPEDWQTGFGEALAQTIGRGIERSTRQSDDRRLAVLEERSTIA
                     RELHDSIAQSLSYSRIQMHRLKVFIERGEPQEKVMETVNELSEGITTAYRQLREVLTT
                     FRLQISSSGLNGAVEETVEEFRGRTGIATTVSNALLGLELTPNEQIHFVHILREALVN
                     VEKHARASSVRVTLSRGADDMFVLRVQDDGVGIPAAPGRYKHFGLSIMKERAEALHGT
                     LTVAPAEGGGTCVTLVVPERRD"
     gene            7148..7801
                     /gene="narL"
                     /locus_tag="ACZPB6_02450"
     CDS             7148..7801
                     /gene="narL"
                     /locus_tag="ACZPB6_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HIR1183373.1"
                     /translation="MTSKERHTIAIIDDHPLFRRGVRELLDLEQGLDVVGEAGTREEA
                     IKLVRRTEPDLTILDLNMKGCSGVEILSTLKDEDPSRRIVVLTVSDSGKDLTACIQAG
                     ADGYFLKDMEPEEFLKSIRRALEGQTVVDASMLAFLTTTLRAKMSSTLEERPTLTGRE
                     AEVLDLIAKGCTNKVIARRLDITEGTVKGHVKHLLKKLGCRSRVEAAVWAAQQKTAE"
     gene            complement(7900..8211)
                     /locus_tag="ACZPB6_02455"
     CDS             complement(7900..8211)
                     /locus_tag="ACZPB6_02455"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF015828.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HIR1183374.1"
                     /translation="MIKKLAPLFAALCLLSGATVVQAGDTDIIGAPGIIPHPIESYLP
                     ITAERNSCLMCHKNAVAKDRKAGEIPLTHLKDGKITGDRWNCTLCHAPSMPAETNAKA
                     K"
     gene            complement(8204..9079)
                     /gene="napH"
                     /locus_tag="ACZPB6_02460"
     CDS             complement(8204..9079)
                     /gene="napH"
                     /locus_tag="ACZPB6_02460"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HIR1183375.1"
                     /translation="MKRLYNMRWTLARRALQLAVLGLFAGTARLGWTLAGEPLLDGTL
                     SASEVAGTIPLSDPLALLERLAAGHMPTLTAGLGALIVLVLYSLLGSRTFCGWICPMN
                     MVADAAEWLRVRLELKADVVRISNKVRYGLLAAALILSAATGTAAFEAVSPQAWIWRD
                     LVFGTGLAALSAASAVFALDLALMKHGWCGHLCPLGAFWSLVGRLTRSPVVRVSFDDA
                     ACNRCGDCLRACPEPHVIRFASLKETGRIPAGDCLNCGRCIETCGENALKFRIGPAPR
                     IRTSSDTHQGENHHD"
     gene            complement(9076..9843)
                     /gene="napG"
                     /locus_tag="ACZPB6_02465"
     CDS             complement(9076..9843)
                     /gene="napG"
                     /locus_tag="ACZPB6_02465"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HIR1183376.1"
                     /translation="MTGSAKTTRREFLTKACELSAAGFVTAFAWGSLSLRDANARWMP
                     RPPGAVDGKDFLALCSRCGQCVTSCPYETLRLAGPFDPAPGGTPFFVPEEIPCYMCKD
                     IPCVKACPTGALSPALTGISDARMGIAVVDPASCLSHQGLRCEVCYRACPEEEKALTI
                     EMHPRGISRHAMFIPVVHPEHCTGCGLCTKSCPTGTPAIRIADRKAVLGEIGGHYRLG
                     WLPDDDPKNRRGDAPADAPEASAQGAGGLDYLNENAL"
     gene            complement(9855..12596)
                     /gene="napA"
                     /locus_tag="ACZPB6_02470"
     CDS             complement(9855..12596)
                     /gene="napA"
                     /locus_tag="ACZPB6_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HIR1183377.1"
                     /translation="MSVHQVNEKRRALIKSGLAAGAAMSIGIPVTSVQAASARKSEEG
                     ISWHKGVCRFCGTGCGLQVGVRDGRIIATKGDPDAPVNRGLNCVKGYFNAKIMYGRDR
                     LTQPLMRRTNGKFDKNGKFEPVSWDEAFDEMARQFKRVYAEKGPSGVALVGSGQYTIP
                     EAYTATKLWKGGFRSNNIDPNARLCMASAVVGFYQTFGVDEPANNYSDIEFANTMILW
                     GNNMAEAHPVLWSRVANRKLTDPNTKVINLTTYRNMSSDLADETIIFKPNGDLAIANY
                     LIREIISRGAVDEDFVSKHTIFTAGVTDIGYGLRNTDKYAYPAERDILEKQKRIRLSP
                     AEATAMGLKAGTEVEQKNSGGSAGAHWQIEFEEFRKAVEPYTLDFVAPLVKGDDDETL
                     ESFKARLVRLADLVCDRKRDLLSFWCMGFNQHQRGVWANELVYSIHLLMGKHAKPGNG
                     AFSLTGQPSACGSAREVGTFCHRLPSDMLVANGEHRKKTESIWNLPAGTLNPKVGYSV
                     MEIMRGLEDGSVNFMWTQVVNILQSTPNNTHWVRAARDPKNFVVVSDVYPTYSARAAD
                     LILPAAMHFEKWGLYGNAERRTQGWHQLVKAPGEAKTDIWMMMEFARRFTVDELYRAQ
                     PLKGVPGDALPDVREAAAAMGLKGDATLFDVLFAPSAARQAAWPDPLYKKELNATGEA
                     LGLPYFPEKALFGEYRQFTLGNGHDLADFDTYMREDVRGLLWPVVNGRETLYRFNVEY
                     DPYAKPDNLFYGKLMKPVGKGTRHALTDKNAVAHPGTAKIFFRPYMDPVEMPDANYDL
                     WLCTGRILEHWHTGSMTRRVPELDRAAPRAVLYMNPADAERRGIRRGDTATVTSRHGE
                     CKAVVETKVRNIMPRGMTWLAFFDDKVLCNSVVIDAMDPISLEPDFKKTAVKVVRDI"
     gene            complement(12648..12890)
                     /locus_tag="ACZPB6_02475"
     CDS             complement(12648..12890)
                     /locus_tag="ACZPB6_02475"
                     /inference="COORDINATES: protein motif:HMM:NF015861.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HIR1183378.1"
                     /translation="MNYSSVVITLGAESRALKALSALEGLEVHHVDEASRRVVAVIEA
                     ETTGLEADVFERIRRTEGVIDVSLHAHYFEDEATAE"
     gene            complement(13288..14142)
                     /locus_tag="ACZPB6_02480"
     CDS             complement(13288..14142)
                     /locus_tag="ACZPB6_02480"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1183379.1"
                     /translation="MFSSLADKLVLSNGVQMPGIGFGTWQVPTDSTLENAVSAALKLG
                     YRFFDTAQIYGNASAIGRGVLLSDLPRAEIFISSKIWTSHRSYDGVMRAFTDIIEQLK
                     TTYLDQLLIHWPATQGEPMIWQSQNAGTWRALEDLYEQGAVKVIGVSNFLPHHLVPLL
                     ARARIRPMVNQLEIHPGYPQFAAVNFCFKQNIAVQAWSPLGRGGLTHHPLLIELGEKY
                     GVSPALIALRWSLQHGFIPVVKALDAEHMKSNLDAFGLTLEDEDMTRLDTMQQVAFSG
                     LHPDTVTF"
BASE COUNT         2514 a         4285 c         5030 g         2658 t
ORIGIN      
        1 gtacgaggtg aggacgaatg cccgcgcgat cgaggccgtt cggttccaga ccttcgagaa
       61 cagcgtgccc gccctcgagg cgtcgggccg cgccaatccc ggcagcggcc tcacggtcga
      121 caacgtggcc gcctacatgg tccagcttct caatcagacg aagaagaggc ttggcgacag
      181 ggccgttctg cccgccgcac ccgagcccgt tccgactcag gctcccgccg cccggcagcc
      241 ctgaacggga caacaacaag cccgccgctt caagtccgaa gctgcgggct ttttttctga
      301 aaagtcactc gagctcaccc tgagctcttc ggagttttct cctgtcagta gagctgcacg
      361 acgcggtatg gctcggcgtc cctgatgaag gggccgatgg ctgcgctcct gagctccttg
      421 cagtgcttct tcacgatctc tgcgacctcc tggccgaagg gttcgacgtc gatcttgcgg
      481 gcgcggcgac ggaggtactt ggcgatgctc tggctggcgg cgtagtcgga tcctgcggcc
      541 ttgaagaatt cgttgatgaa gagcgcgagg atgagcggcg tgtcgacatt cctgtccgcc
      601 ttgaagctgt tgaggtcagc gcagagattc gcgtcgaacc ccagggacct gaggccgttg
      661 acggactggg gatgaagctc gcggggcagg aagcgcaggt tgttgagaag ctgttcggag
      721 cgggtgggga gcgtggcgcg gagcatgagc tccatgggaa gcgtgatgtt tgacatggcg
      781 gactgaaacg gaagtacgga actcgaacga atgttgacgg cgagggcgcg ctgcaacgcg
      841 atgcttcgcc ttggattcga gtatggggca gttcgccgaa ggacatcgaa ctatttgaag
      901 gttgcatgac ctgcgccaaa agtgccgtat cagaatcgtg aaatgatcct gtgaaaagtg
      961 cgggttttgt cgataaaaat gttgcgccac aatggttttg gtggtgtttc cactggatca
     1021 tgccgatgca cggcattccg tcgtcccttt tctaggttgt ttgcagtaga gcgaaaagtg
     1081 cgatcttaag gtagaatcgt cggtacttcg ccggatccgc gggaaccggc agggcttcca
     1141 gaacaagaca tgcagtcggg gcaccggatc ctctccggtc aacttccttt caacgccctc
     1201 gcatgccgga ggcttttgtc ctgtttctca ttattcaagg tggattaatc atgaaattca
     1261 agcttttcgc ttctgtgtgc gtggctgccg gcctcgcctt cggtcttgcc ggctgcggtg
     1321 acgacaatgc tcccaagagc ggcgacgcca agaccctcac ggtcggtgcg agccccgttc
     1381 cgcacgcgga cattctgaat ctcatcgccc cgcagctcca gaaggagggc atcacgctca
     1441 aggtgatcga gttctccgac tacattcagc cgaacctctc gctcaacgac aaggagcttg
     1501 acgccaactt cttccaacac aagccctatc ttgactcctt cagcaaggac cgcggcctca
     1561 agctcacgag cctcgtcgcc gttcacatcg agccgatggg tgtgtactcc cacaaggtga
     1621 agaacgttgc cgacgttgcc gagggctctg aagtcgccat tccgaacgac ccgaccaacg
     1681 gcggccgcgc cctcaaggtg ctcgagacgg ccggcctcat caaggttcgt cctgaagccg
     1741 gcgtgacggc cacgatcaac gacgtggtcg acaatcccaa gaagatcaag ttccgcgaga
     1801 tcgaggccgc ccagcttccg cgcgcccttg acgatgtcgc gctcgccgtc atcaactcca
     1861 actacgccct gggcgccggt ctcaacccgg tgaaggacgc ccttgccatc gaaagcaagg
     1921 attcgcccta cgccaacatc gtggccatcc gcacgggtga cgacagccgc gccgagatcc
     1981 agaagctcaa ggccgctctg acgtccgccg aagtcaagaa gttcatcgaa accgaataca
     2041 agggtgccgt ggttccggcc ttctgatcct ggcattgaac ctctgaacaa tcagggcccg
     2101 ccgcagcatc gtctgcaggc gggccttttt ctttccgggc gcgcctattc gtcggcgggc
     2161 ggcttcatcg agcagcagga cttccggttc cttttcgcca gataggcgtc gccccagcgg
     2221 tacatggccg tgagaatggg tttgatgctc tcgcccgtgg gcgtgagcgc gtagtcgacc
     2281 cgaggcggaa cgacgggcca gacggtgcgc gagacgaggc cgtccgcctc gagttcgcga
     2341 agctgctgcg tgagcatctt gggcgtggcg tcgggcacgg cctttctcag cgccgagtag
     2401 cgcatcgtgc cttcgagcag gttccagaga atgagcgcct tccacttgcc gccgatgagg
     2461 ctgagcgtga gcgcgacggg acagagcact tctcctgcag gcgttttgtc ggtcatgacg
     2521 gtatcctttt gggtactgga tatcaaaatt gtgcgttctt gcgaaaagga acttgtcaag
     2581 gatacactcc ctgaaccgaa taatcattct cattcagaac gatggaaacc agacagttca
     2641 acgtgacggg catgagttgc gcggcctgct cgagccgcgt ggagaaggcc gtgtgcgccc
     2701 ttcgcggcgt gagtgcggcc gaggtgaacc tgctcacgaa ttcgctcaag gcgacgtttg
     2761 acgaaggcgt gctcggcgtc gaggacatcg agcgtgcggt ggaagatgcg ggctacgggg
     2821 cgagcgagaa gggctttgcg gggcgaggga ggactgcggc cgcgacggcg cccgaggcgg
     2881 acgcggaggc ggcggccgaa gccgtgcgcg tgcgcctcat ctggtccttt gcgctgctcg
     2941 cacccctcat gtggctttcg atggggccga tggcgggcct gcccgtgccc gtcatgctca
     3001 cgggggcgca gggcgctccg atgatggcct tcacgcagtt cctgctcacg ctgcccatcg
     3061 tgcttctcaa ccgccgctac ttcacgggcg gcctcagggc gctcgtcaac cgcgcgccca
     3121 acatggacag cctcatcgcg gttggatcgg gcacagcgct gctctttggc gtctacgtga
     3181 tctacaggct tcttgcggcc caggctgcgg gtgacgaagc ggcgctccac cactttgcgc
     3241 acagcctcta cttcgagggg gcggcgacga tcgtcacgct catttcgctc ggcaagttct
     3301 tcgaggcgcg cgccaagcgc cgcacgaccg aggcggtgac ggcgctcatg aagcttgcgc
     3361 cgccgacggc cctcgtggtg agggacggcg cggaggtccg cattggaacg gatgaggtcg
     3421 cctcgggtga catcgtcgtc gtgaagacag gcgagacggt gcccgtcgac ggcgtgattc
     3481 tggagggaac tgcgcttctg aacgagtcgg cccttacggg cgagagcctt cccgtgacga
     3541 gagggccggg cgaggccgtg acgggcgcga cgcttctggc ggcgggcgcc ttcagaatgc
     3601 gcgccacgcg cgtgggcgac gacacggtgc tggcgggcat catcaggctc gttgaggagg
     3661 cgacggcctc gaaggctccc gtggcgaggc ttgccgacaa ggtgagcggc atcttcgtgc
     3721 ctgtcgtgat gacaatcgct ctggtgtcgg gccttgtctg gtactggatc ggcggagacc
     3781 tagagttcgc gctcaacgcc gcggtgagcg tcctcgtcat ttcctgcccg tgcgcgctcg
     3841 gactggcgac gcccacggcc gtcatggtcg gcatgggccg aggcgcaggc atgggcgttc
     3901 tcttcaagag cgccgaggcg ctcgaggtct tctccgggct caagaccgtc gtcttcgaca
     3961 agacgggcac gctcaccgag ggcaggcccg tcgtcacgaa ggtggcggca gcggtgcccg
     4021 gactcgaaac cgtggtgatg ctcgtcgcag ccgcgcttga aaagccttcc gagcatccgc
     4081 tcgcgcaggc ggtgctcgcc gaggccgagg cgaggggact gaccgcgcag gatgtcgatg
     4141 agttcgagca gattccgggc ggcggcctca ggggcctgat gtcgggcacg ccggtggcgg
     4201 cgggcaacgc ccgtctcatg gagacgctcg gccttgaggt gcccgaggcg ctttccgcgc
     4261 tcgcactcgc agaggcctcg cagggcgcga cgccgctctt cattgcttcg gcagggagcg
     4321 ttatcggcat catcgccgtc gccgacaggg tgaggtccga atcgagggcc gccgtctcgg
     4381 cgctgcgcgg acgcggcctc agggtcgtga tgctcacggg cgacaaccgc cgcacggccg
     4441 aggccgtcgg cgcaaggctc ggcattgcgt ccgaggacat cgtcgccgac gtgaggcccg
     4501 ccgaaaaggc ctcgcacgtg aaggcgcttc aggccgacgg accgtgcgcc atggtgggcg
     4561 acggcgtcaa tgacgcgcct gcgctcgccc aagccgatgc gggcgttgca gtgggcggcg
     4621 gcacggatgc cgcgcgcgcg agcgccgacg tcgtcctcat gcgtgattcg cccgagggcg
     4681 tcgtgggcgc agtggatctt tcccgcgccg tcatgcgcaa catcaggcag aacctcttct
     4741 gggcgtttgc ctacaatgtg atcggtattc cgatcgcggc aggcgtcctc tatcccatgt
     4801 tcggtctcct cctcaatccg atgattgcgg cggcggccat gagcatgagc tcggtgagcg
     4861 tcgtcacgaa cgcgcttcgc ctccgattct tcaaggcgcc cgtcctcgtc tccgacagcg
     4921 ggacggacgg cgcatccact acagggagtt ccatgatgga aaaggtgatt cacattgaag
     4981 gcatgcactg cggtcactgc acggccgctg tcgagaaggc gcttcgcgcg ctgcccggcg
     5041 tcgagtccgc ggtggcggac cttgagaaga aggaggcccg cgtcaaggct gcagccggcg
     5101 tctccgacgt gatgctctcg gccgtcgtga cgggcgcggg cttcaaggtg acgggcatcg
     5161 acacgctctg agcctgagaa cgcacggagc attctgcagg gggctctcca cattgatgga
     5221 gcggcccctt cgcttttcct gcatcatctt tcgtcccgca ccggcgcctt tgtaaaatcg
     5281 aaggatccgc ccgtgcaacc tccgcttcaa ggatccgcca tgcgcatcat caaatggctt
     5341 cgcgcctcca ttgtccgaca gtgcttcgtc gttctggccg tgatcggcct tctggccgta
     5401 ctcgccgcgg cccccgctct gattctcacg gagcgctcga cgggatctgg gggcgccatc
     5461 aacgtctcgg gcagcatgcg catgcagagc tacaagcttg cgcttgccgt tgcggatcct
     5521 ttctcctcct tcgagcagcg aagggagaag accatgacgg cgctcgacga gttcggcgtc
     5581 aagctcatga gcccgggcct tctcaacggc gtgccccacg attcgggcaa tcccgcctac
     5641 atccagtacg tagccctcaa ggatagattc gaaaagggcg tgcgtccact tgcggaggcg
     5701 agcatcgaca gtcttgaggc caggcggcgc ttcacggagc agattccttc gttcgtggag
     5761 gacgtcgacc ggttcgtcca gacgcttgag aacgggctca acgagcgtct tgcactcctg
     5821 cagaagatcc tcaccctcat tctcttcggc gccatgggcg tgagcttcgt gatgcttctc
     5881 gtcatgcaga ggagcatctt caggccgttg ctggccatcg gctcggctgc cgatgcggtg
     5941 agaaagggcg acttctcggt gagggcgaag gtcccggcgc ccgatgagat cggcaggctc
     6001 gcgacgagct tcaacttcat ggtcgacgag ctcggccgtc tttacggcaa tcttgagcat
     6061 gaggtcgaga agaagaccga ggatctcaac cgccgcaacg caggtcttca gttcctgagc
     6121 cgcgtggcgc agtcggcggc ctggggcgcg gtcttcaagc gccattcgct cgacgacctg
     6181 ctcgaggagt cctgcgtaca gttcggagcg agagccgtga ggctcgtcgt cagcacggac
     6241 ggtacggccg cggttctcgg agaatcggac gcttggcctg aggacgaacc cgagacgggc
     6301 gtcgtgcgct ttgtactctc caatgccaat ccgtcgatgg gcacgctcga ggcggtcttt
     6361 gatgcagagc ccgaggactg gcagaccggt ttcggcgagg cgcttgcaca gacgatcggg
     6421 cgcggcatcg agcgcagcac ccggcagtcg gacgacaggc gtctggcggt gcttgaggag
     6481 cgctcgacga tcgcgcgcga gctccacgac tcgatcgcgc agtcgctctc ttattcgcgc
     6541 attcagatgc accgcctcaa ggtcttcatc gagcgcggcg agcctcagga gaaggtgatg
     6601 gagactgtca acgagctttc cgagggcatc acgacggcct acaggcagct tcgcgaggtg
     6661 ctcacgacct tcaggctaca gatcagcagc tcgggtctca acggcgccgt cgaggagacg
     6721 gtcgaggagt tccgcggccg cacgggcatt gcgacaacgg tttcgaacgc acttctcgga
     6781 ctcgagctca cgcccaacga gcagatccac ttcgtgcaca tcctgcggga ggcactcgtc
     6841 aacgtcgaga agcatgcgcg tgcgagcagc gtacgcgtga cgctttcgcg cggagcggac
     6901 gacatgttcg tgctcagggt tcaggacgac ggcgtcggca tccccgctgc ccccggacgc
     6961 tacaagcact tcgggctctc gatcatgaag gagcgcgccg aggcgctgca cggcacgctc
     7021 acggtggcgc cggccgaggg tggcggcacc tgcgtcacgc tcgtcgtccc cgaaagacgg
     7081 gactgaaggc gcctgcgtgc agcggtagaa ttaaagaatg caattttctt cagggaacgg
     7141 ccagcaaatg accagcaagg aacgccatac gatcgcgatc atcgacgacc atccgctctt
     7201 tcgccgcggc gtgcgcgagc tcctcgacct agagcagggg cttgacgtcg tgggcgaggc
     7261 gggcacgcgc gaggaggcga tcaagctcgt cagacgcacc gaacccgacc tgacgatcct
     7321 cgacctcaat atgaagggct gctcgggcgt ggagatcctc tcgacactca aggacgagga
     7381 tccgtcgcgc cgcatcgtgg tcctcacggt gagcgactcg ggcaaggacc tgacggcctg
     7441 catccaggcg ggcgcggacg ggtacttcct caaggacatg gagcccgagg agttcctgaa
     7501 gtcgatccga agggcgctcg aggggcagac cgtcgtggat gcgtcgatgc ttgctttcct
     7561 cacgaccacg cttcgcgcga agatgtcctc cacgcttgag gagcgaccca cgctcacggg
     7621 gcgcgaggcc gaggtgctcg acctgatcgc caagggctgc accaacaagg tcatcgcccg
     7681 caggctcgac atcacggagg gcaccgtcaa gggccacgtg aagcatctgc tcaagaagct
     7741 cggctgccgc agccgcgtcg aggccgccgt ctgggccgca cagcagaaga ccgccgaatg
     7801 aaaggcgacg gatgacgaat gaacaaaggc cgcaggagcg aatctcctgc ggcctttgtt
     7861 ctttgaggat gcgtgccgcc cgattgggcg acgcagcgct tacttggcct tggcgttggt
     7921 ttcggcgggc atgctcggcg cgtggcagag cgtgcagttc cagcggtcgc ccgtgatctt
     7981 gccgtccttg aggtgggtga gcgggatttc acccgccttg cggtccttgg ccacggcgtt
     8041 cttgtggcac atgaggcagc tgttgcgttc ggccgtgatc gggaggtagc tctcgatcgg
     8101 atgcgggatg atgcccggcg cgccgatgat gtcggtgtcg cctgcctgga cgacggtggc
     8161 gcccgagaga agacagaggg ccgcgaaaag cggggcgagt ttcttaatca tgatggtttt
     8221 ctccttgatg ggtatcggag gaagtcctga tcctgggggc ggggccgatg cggaacttga
     8281 gcgcgttttc gccgcaggtc tcgatgcagc ggccgcagtt gaggcagtcg ccggcgggaa
     8341 tgcggcccgt ttccttcagg ctggcgaagc ggatgacgtg gggttcggga caggcgcgca
     8401 ggcagtcgcc gcagcggttg caagctgcgt cgtcaaagct gacgcgcacg acgggggagc
     8461 gcgtcaggcg accgacgagc gaccagaagg cgccgagcgg gcagaggtga ccgcaccagc
     8521 cgtgcttcat gagcgcaagg tcgagcgcga agaccgcgga ggcggccgag agcgctgcga
     8581 gccccgtgcc gaagacgaga tcacgccaga tccaggcctg cgggctcacc gcctcgaagg
     8641 cggccgtgcc cgtggcggcg ctcagaatga gcgcggcggc gagaaggccg tagcggacct
     8701 tgttcgagat gcgcacgacg tcggccttga gctctaggcg aacgcgaagc cactcggcgg
     8761 cgtcggccac catgttcatg gggcagatcc agccgcagaa ggtgcgcgag cccagaaggc
     8821 tgtagagcac gaggacgatg agggcgccga gtccggccgt gagcgtcggc atgtgccccg
     8881 ccgcaaggcg ctcgaggagc gcaagcgggt cggagagtgg aatcgtgccg gcaacttcgg
     8941 aggccgagag cgtgccgtca aggagaggct cgcccgcgag cgtccacccg aggcgcgccg
     9001 tgcccgcaaa gagcccgagc accgcaagct gaagtgcgcg gcgggcaagc gtccaacgca
     9061 tgttgtagag ccgcttcata gcgcattctc attgaggtag tcgaggccgc ctgcgccctg
     9121 ggcggaagcc tcgggggcgt ctgcgggggc gtccccgcgc cggttcttcg gatcatcgtc
     9181 gggtagccag ccgaggcggt agtgaccgcc gatctcgccg agcacggcct ttctgtccgc
     9241 aatgcggatg gcgggcgtgc ccgtggggca gctcttcgtg cacaggccac agcccgtgca
     9301 gtgctcgggg tgcacgaccg ggatgaacat cgcgtgacgg ctgatgccgc gcggatgcat
     9361 ttcgatcgtg agcgccttct cctcctcggg gcaggcgcgg tagcacacct cgcagcgaag
     9421 tccctggtgc gagaggcacg aggcggggtc gacgacggcg atgcccatgc gggcgtcgct
     9481 gatgccggtg agcgcaggcg agagcgcacc cgtcgggcag gccttcacgc aggggatgtc
     9541 cttgcacatg tagcagggga tctcctcggg aacgaagaag ggcgtgccgc cgggagcggg
     9601 atcgaacggc cccgccagcc tgagcgtttc gtacggacag gacgtcacgc actgcccgca
     9661 gcgcgagcag agtgcgagga agtccttgcc gtcgacggcg ccaggaggcc ttggcatcca
     9721 gcgcgcgttg gcgtcgcgaa ggctgagcga gccccaggcg aaggccgtca cgaagcctgc
     9781 agccgaaagt tcgcaggcct tcgtgaggaa ttcgcggcgg gtggtcttgg cgcttcccgt
     9841 catgaggact ccggtcagat gtcgcggaca accttgaccg cggtcttctt gaagtcgggt
     9901 tcaagggaga tcgggtccat ggcgtcgatg acgacggagt tgcagagcac cttgtcgtcg
     9961 aagaaggcga gccaggtcat gcctcgcggc atgatgttgc gcaccttggt ttcgacgacg
    10021 gccttgcact cgccgtggcg gctcgtgacg gtggcggtgt cgccgcgcct gatgccgcgg
    10081 cgctcggcgt cggccgggtt catgtagagc acggcgcgcg gagctgcacg gtcgagctcg
    10141 ggcacgcggc gggtcatcga gcccgtgtgc cagtgctcga ggatgcggcc cgtgcagagc
    10201 cagaggtcgt agttggcgtc gggcatctcg accgggtcca tgtagggacg gaagaagatc
    10261 ttggcggtgc cgggatgtgc gacggcgttc ttgtccgtga gcgcatggcg cgtgcccttg
    10321 ccgacgggct tcatgagctt gccgtagaag aggttgtcgg gcttggcgta cgggtcgtac
    10381 tcgacgttga agcggtagag cgtctcgcgg ccgttgacga cgggccagag caggccgcgc
    10441 acgtcctcgc gcatgtacgt gtcgaagtcg gcgaggtcgt ggccgttgcc tagcgtgaac
    10501 tgacggtatt cgccgaagag cgccttttcc gggaagtacg ggaggccgag ggcctcgccc
    10561 gtggcgttga gctccttctt gtagagcggg tcgggccagg cggcctgacg ggctgcggac
    10621 ggcgcgaaga gcacgtcgaa gagcgtggcg tcgcccttga gacccatggc cgcggcggct
    10681 tctcgcacgt cggggagcgc atcgcccggg acgcccttga ggggctgcgc acggtagagc
    10741 tcgtcgaccg tgaagcggcg ggcgaactcc atcatcatcc agatgtcggt cttcgcttcg
    10801 ccgggcgcct tgacgagctg gtgccagccc tgggtgcggc gttcggcgtt gccgtagagg
    10861 ccccacttct cgaagtgcat ggcggcgggc aggatcaggt cggccgcgcg ggcggagtag
    10921 gtcggataga cgtcggagac gaccacgaag ttcttcggat cgcgggcggc gcgcacccag
    10981 tgggtgttgt tgggcgtcga ctggaggatg ttgacgacct gggtccacat gaagttgacg
    11041 gagccgtcct caaggccgcg catgatctcc atgacggagt agccgacctt ggggttgagc
    11101 gtgccggcgg gcagattcca gatgctttcg gtcttcttcc tgtgctcgcc gttggcgacg
    11161 agcatgtcgg agggaagacg gtggcagaac gtgccgacct cgcgggcaga gccgcaggcg
    11221 gaaggctggc ccgtgagcga gaaggcgccg ttgcccggct tggcgtgctt gcccatgaga
    11281 agatggatcg agtagacgag ctcgttggcc cagacgccgc gctggtgctg gttgaagccc
    11341 atgcaccaga agctcagcag gtcgcgcttg cggtcgcaga cgaggtcggc gaggcgcacg
    11401 aggcgggcct tgaagctttc gagcgtttcg tcgtcatcgc ccttgacgag cggggcgacg
    11461 aagtcgaggg tgtatggctc gacggccttg cggaactcct cgaactcgat ctgccagtgg
    11521 gcgccggcgg agccgcccga gttcttttgc tcgacctcgg tgccggcctt gaggcccatg
    11581 gcggtcgcct cggcggggct caggcggatg cgcttctgct tttcaagaat gtcgcgttcg
    11641 gcggggtagg cgtacttgtc ggtgttgcgc aggccgtagc cgatgtcggt gacgccggcg
    11701 gtgaagatgg tgtgcttcga gacgaagtcc tcgtcgacgg cgccgcggct gatgatctcg
    11761 cggatcaggt agttcgcgat agcaaggtcg ccgttgggct tgaagatgat ggtctcgtcg
    11821 gcgaggtcgg acgacatgtt gcggtaggtc gtcaggttga tgaccttcgt gttcgggtcc
    11881 gtgagcttgc ggttggccac gcgcgaccag agcacggggt gggcctcggc catgttgttg
    11941 ccccagagga tcatcgtgtt ggcgaactcg atgtcgctgt agttgttggc gggctcgtcg
    12001 acgccgaagg tctggtagaa gcccacgaca gcggacgcca tgcagagtcg ggcgttcggg
    12061 tcgatgttgt tggagcggaa gccgcccttc cagagcttcg tggcggtgta ggcctcggga
    12121 atggtgtact ggccggagcc cacgagcgcg acgcccgagg gccccttttc ggcgtagaca
    12181 cgcttgaact ggcgcgccat ttcgtcgaag gcctcgtccc aggagacggg ttcgaacttg
    12241 ccgttcttgt cgaacttgcc gttggtgcgg cgcatgagcg gttgcgtcag acggtccctg
    12301 ccgtacatga tcttggcgtt gaagtagccc ttgacgcagt tgaggccgcg gttgacgggc
    12361 gcgtcggggt cgcccttcgt ggcgatgatg cggccgtcgc ggacgccgac ctggaggccg
    12421 cagcccgtgc cgcagaagcg gcacacgccc ttgtgccaag agatgccctc ttcggacttg
    12481 cgggcgctgg cggcctgaac ggacgtgacg ggaatgccga tcgacatggc ggcgcctgcg
    12541 gcgaggccgg acttgatgag ggcgcggcgc ttctcgttga cttgatggac tgacatggag
    12601 gtctcctttg cttgaatgct gtcggggtct gtcgtgaagg gaagggacta ctcggctgtc
    12661 gcctcgtcct cgaagtagtg ggcgtgcagg ctgacgtcga tgacgccttc ggtgcggcgg
    12721 atcctttcga agacgtcagc ctcgagtccc gtggtctcgg cctcgatgac ggcgacgact
    12781 ctgcgggacg cctcgtcgac gtggtggacc tcgagtcctt cgagggcaga aagtgctttg
    12841 agcgcacggc tttcggcgcc gagcgtgatg acaacggagg agtagttcat tcggtggcct
    12901 ttttcacgtt ccgatgagct gattgtaggg agctgcgcac gcaccgccta gacgcttgaa
    12961 gagaggtgcg ggaggctctt tggagatagg ggttggcccc gtggcagcat ctttccctga
    13021 aggcggtgag gcggctcgaa gccctgattt gactgtggga gaggtaatga cgctattctg
    13081 taatagaatt gcggtgctac caccaaagag gtatcgactt ggtccgatgg agatgggcgg
    13141 cgtttccctc gggcgagaat gttcttctct gaagtcagac tggctcgaaa ttttcaagag
    13201 gtacaaaaac cgacctgaga gcatgtcggt tttcgtttac caaaaagccc gccttcgtga
    13261 agggcgggct ttcgtggctg ggcgtcgtca gaaggtgacg gtgtcggggt gcagtcccga
    13321 gaaggcgacc tgctgcatcg tgtcgaggcg cgtcatgtcc tcgtcctcga gtgtgaggcc
    13381 gaaggcgtcg agattggact tcatgtgttc cgcatcgagt gctttcacga caggtatgaa
    13441 gccgtgctgc aggctccagc gcagggcgat gagggcgggg gagacgccgt acttctcacc
    13501 aagttcgatg aggagcgggt ggtgtgtgag accgccgcgg ccgagcgggc tccaggcctg
    13561 aacggcaatg ttctgcttga agcagaagtt gacggccgcg aattgcggat agccggggtg
    13621 gatctcgagc tggttgacca tcgggcggat gcgcgcgcgg gcgagcagcg gcacgaggtg
    13681 gtgcggcagg aagttcgaga cgccgatcac cttgaccgcg ccctgttcgt agaggtcttc
    13741 gagggcgcgc caggtgcccg cgttctggct ctgccagatc atgggctcgc cctgagtggc
    13801 gggccagtgg atgaggagct ggtcgaggta cgtggttttg agctgctcga tgatgtcggt
    13861 gaaggcccgc atcacaccgt cgtagcttcg gtgcgacgtc cagatcttgc tcgagatgaa
    13921 gatctcagcg cgcggcagat cgctcagcaa cacgccgcgg ccgatggcgg aggcgttgcc
    13981 gtagatctgt gcggtgtcga agaagcggta gccgagcttg agggccgccg agacggcgtt
    14041 ttcaagcgtg ctgtcggtgg gcacctgcca agtgccaaag ccgatgcccg gcatctggac
    14101 gccgttggaa agcacgagct tgtcggcaag ggacgagaac acgggaaaac tcctctgctg
    14161 tagggcggcc gcggatccgg acggcctggt ggttgaatgg aaccagatca ttgtagatta
    14221 gcttttgaga aaaagtcttg acgaatgtcg aaggcgttca acgcaaacga ctggggaaaa
    14281 caggaaaaac agtcttcggc atgtaccaca agcgccctga aagacgcata attgaaagtc
    14341 gacgctatca ttcggacgaa gcgctccaca accggccgcc ctcgcgccgg acggcgcgga
    14401 ctccgcaact gcaactacac agacaagaag agaaggtcaa taacccccgc ctaaaggcgg
    14461 aggcttgaaa gagcctttat tgactag
//
LOCUS       DBMDTI010000024        14429 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1315_length_14429_cov_3.413596, whole genome shotgun sequence.
ACCESSION   DBMDTI010000024 DBMDTI010000000
VERSION     DBMDTI010000024.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 14429)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14429)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14429
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1315_length_14429_cov_3.413596"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="ACZPB6_02485"
     CDS             <1..389
                     /locus_tag="ACZPB6_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013857.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR1183380.1"
                     /translation="GISDSILVFDQHRTLTRNPVHAVRRLMAMEPERKVCVEVDTPED
                     GMFWADKGVHIIQCERFTPEVLAAFVREVKAKHPHVVVNAAGGINGDNVGDYADTRCD
                     VLVTSWPYFGRPADIKMSFSPANPKP"
     gene            386..1006
                     /gene="recR"
                     /locus_tag="ACZPB6_02490"
     CDS             386..1006
                     /gene="recR"
                     /locus_tag="ACZPB6_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR1183381.1"
                     /translation="MSIRLSPAVESLVKALGKLPGYGPRSAQRAAFHLLQNRDESLDE
                     LAAAVERVRRGVGRCRLCNTFTEGDICPVCLDEERDPGLLCVVESTADQMALDASLAW
                     PGYYFVLSGRLSPVNGIGPADIGMPFLLKRVGAGVEEGVLREVVVAMSYTPEGDATAY
                     YLIDALKKRWPQLRVTRLARGLPAGIEIEYTDLSTIANAVYARRDA"
     gene            1281..2468
                     /gene="carA"
                     /locus_tag="ACZPB6_02495"
     CDS             1281..2468
                     /gene="carA"
                     /locus_tag="ACZPB6_02495"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR1183382.1"
                     /translation="MHSLFKDPRPEAALILSDGTVLLGRSFGASVSNVAEVVFNTAMT
                     GYQEVLTDPSYTGQIVTLTGAHVGNVGVNAQDREGGAAVHAAGLVVRDVPAVHSNFRS
                     EKSLPDYLVEQGVPAIAGVDTRRLTRILRTKGAQAGVIVVARNGALTEDEIDAGRKAV
                     AAWKGMAGQDLAKVVTCKAPYEWTEGAFSMKAADGRPGFVTPAELPVHVVAYDFGVKT
                     NILRMLVERGMRVTVVPAQTPVEDVLAMNPDGVFLSNGPGDPAPCTYAIEAAREIIAR
                     KIPLFGICLGHQIMGLAVGAKTLKMKFGHHGANHPVVDLRSRHVYITSQNHGFAVDAK
                     TLPANAVPTHESLFDGSLQGFELTDAPAFCFQGHPEASPGPHDIAPLFDHFRELIERH
                     RAA"
     gene            2508..3098
                     /gene="gmhA"
                     /locus_tag="ACZPB6_02500"
     CDS             2508..3098
                     /gene="gmhA"
                     /locus_tag="ACZPB6_02500"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HIR1183383.1"
                     /translation="MEKANVLAALNDARDALDALISNEKTIEAVVAAAGLMADAVEGD
                     GKVMSCGNGGSLCDAMHFAEEMTGRYRSNRRPYAALAISDASHMACVGNDYGYEEVFS
                     RYVEAHGRKGDVLLAITTSGTSRNIVKAAEVARRKGVKVVALTGRDETPITELADVSI
                     VTPAGRWADRVQELHIKCIHILIELIERRLDKQNYE"
     gene            3102..6326
                     /gene="carB"
                     /locus_tag="ACZPB6_02505"
     CDS             3102..6326
                     /gene="carB"
                     /locus_tag="ACZPB6_02505"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR1183384.1"
                     /translation="MPKRTDIKSVLIIGAGPIIIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPQTADVTYIEPITWEVVERIIAKERPDAILPTMGGQTALNCAMDL
                     ARHGVLDKYDIELIGAKPDAIDKAEDRERFKEAMDRIGLESARSGVAYSLREAKELQA
                     KFGFPTVIRPSFTLGGSGGGIAHDMESFIEICERGLELSPTHELLIEESLLGWKEYEM
                     EVVRDTADNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDREYQRMRDASIAILREIG
                     VDTGGSNVQFAVNPETGRMIVIEMNPRVSRSSALASKATGFPIARIAAKLAVGYTLDE
                     LRNEITGGLTPASFEPTIDYVVTKVPRFAFEKFPAANDRLTTQMKSVGEVMAIGRTFQ
                     ESLQKALRGLETGHNGLNETGVGIEQLRHEIAVPGPERIFFLADGIRAGMTDKELHEL
                     SAIDPWFLNQVREIVETEDKLRGNPFSAFTKSDMLFLKRKGFSDGRLAQLTGAKEAEV
                     RAARLAMGIRPVYKRVDTCAAEFATNTAYLYSTYEEECEAEPTDRRKVMVLGGGPNRI
                     GQGIEFDYCCVHASKALRADGWETIMVNCNPETVSTDFDTSDRLYFEPVTLEDVLAIC
                     EVEKPAGVIVQYGGQTPLKLCRALEAAGVPIIGTTPDAIDCAEDRERFQHLLQKLNLR
                     QPPNRTARTEEDAIRLAAEIGYPLVVRPSYVLGGRAMDIVHTPEALQRYMKEAVSVSN
                     ESPVLLDRFLDDAVEMDVDAVADGHEVQVAGVMEHIEQAGVHSGDSACSLPPYSLPAV
                     VVEEIKRQTKLMAEALNVVGLMNVQFAVQHAHSENPVIYVLEVNPRASRTVPFVSKAT
                     GNPVAAIAARVMAGQTLAEQGVNLEVTPRYVSVKEAVFPFSKFLGVDPVLGPEMRSTG
                     EVMGVGRDFGEALFKSQLAAGSRLPERGSVFISVRECDKPKAVVCAHQLHQAGFPLVA
                     TAGTAHVIQQAGIPCRTVGRIGDAAGDVIGMMEAGDITLVIMSVAEHEGELNDARAIR
                     KLALAKQITYYTTMAGGLAASEGIRHMRSVQVYDLQGLHAGTLP"
     gene            6464..6946
                     /gene="greA"
                     /locus_tag="ACZPB6_02510"
     CDS             6464..6946
                     /gene="greA"
                     /locus_tag="ACZPB6_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR1183385.1"
                     /translation="MVSTRIPITTRGAEKLKAELDYLKKVARPNVVAAIAEAREKGDL
                     SENAEYDAAREEQGHIEGRIAELEGKIPALQIIDPKSVDAGERIVFGATVGLYDEDDD
                     VSVTYQIVGDDEADIKENRISVSSPIARALIGKYEGDSVQFMAPKGLVSYEIESVEYI
                     "
     gene            complement(7032..7562)
                     /gene="yhbY"
                     /locus_tag="ACZPB6_02515"
     CDS             complement(7032..7562)
                     /gene="yhbY"
                     /locus_tag="ACZPB6_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIR1183386.1"
                     /translation="MKEIILARDARLALRSQAHHLDPVVLLGANGLTNAVLHEIDRAL
                     NDHELIKVRVPSDDREELESIYAEVAEKLGAARVQMIGKLLIFWRPADESERQDPDEA
                     ARLAILSMKTASVAGKKEAPSAPAKRTPLKAVAKPAKKVASKKPGEKKKATSPIPVRS
                     RSKKNRVTKKGAGSRS"
     gene            7645..8298
                     /locus_tag="ACZPB6_02520"
     CDS             7645..8298
                     /locus_tag="ACZPB6_02520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HIR1183387.1"
                     /translation="MPVATKKLRSNRAWIERHINDPFVKRSKAEGYRARSVYKLTELD
                     DREHLLRRGMTVVELGAAPGSWTQIVRERLSDKDGRVQGRIIAMDILPMDPIDGVTFL
                     QGDFREQEIADKLAEILDGDKVDVVLSDMAPNLSGIAAADAARSLLLNELAHEFCLEH
                     LKPNGVFVTKSFQGSGFSQFVEALKKTFKKVYTRKPAASRDTSAEVYLVARELKPGK"
     gene            8522..10531
                     /gene="ftsH"
                     /locus_tag="ACZPB6_02525"
     CDS             8522..10531
                     /gene="ftsH"
                     /locus_tag="ACZPB6_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1183388.1"
                     /translation="MDNKIFSRIAVWALVGLVLFTVFRQYEDAYAPPRDTISYTQFME
                     DAKAGKVRRVDIQGRTLIVTPQTGEEFKLTNPGDIWMVDELRKSNVQIYGKAEEEPSL
                     LTTIFISWFPMILLIGVWIFFMRRMQGGAGGGGGAFSFGKSKARKLDEESNKVRFRDV
                     AGCNEAKEDVQEIVDFLREPKRFQRLGGRIPRGLLLVGPPGTGKTLLAKAIAGEAGVP
                     FFTISGSDFVEMFVGVGAARVRDMFETAKKNEPCIIFIDEIDAVGRQRGAGLGGGNDE
                     REQTLNQMLVEMDGFDSGANVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGRD
                     QILNVHMRKIPVGKDVDSMVIARGTPGFSGADLANLVNEAALFAARRNGRVVKMCDFE
                     NAKDKIMMGAERRAMVMSEDERRNTAYHESGHAVVARLMPKSDPVHKVTIVPRGRALG
                     LTMQLPKEDRYAYDRQYLLTRIAILFGGRIAEEVFMNQMTTGASNDFERATQMARDMV
                     MRYGMSDRLGPMVYSENEGEVFLGRSVTKTTNISEKTMQEVDAEVRRIIDEQYAIARR
                     LIEENRDKMEVMAKALLDWETIDADQIDDIMEGRAPRPPKTSKEAVKPEAAQAAAPEG
                     EAGAAEEPKAEDAEAPAAPEAAGAKEASSSDNAGAPKGDDDRRSE"
     gene            10620..11474
                     /gene="folP"
                     /locus_tag="ACZPB6_02530"
     CDS             10620..11474
                     /gene="folP"
                     /locus_tag="ACZPB6_02530"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR1183389.1"
                     /translation="MTELHERASVWRCGAKVFEIDRLHPLIMGILNVTPDSFSDGGTH
                     NGLEDAVAWAHRMRDEGAHIIDVGGESTRPGFNETGVTLEEERRRVIPVVEQLVKEGF
                     IVSVDTSKPEIMTEVASLGAHILNDIRGFEMPGALEAAAATDCGLVVMHRSATTDYKD
                     VVAEVEAYLQERQRLLEGLGVSADRICWDPGFGFGKTVEQNFALLGSTERFVASGQPY
                     LMGLSRKSSIGAATGHKAPMDRIAGSIAGALIAVEQGAQVVRVHDCRQTVDAVAVWRA
                     SEEACGRR"
     gene            11498..12844
                     /gene="glmM"
                     /locus_tag="ACZPB6_02535"
     CDS             11498..12844
                     /gene="glmM"
                     /locus_tag="ACZPB6_02535"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR1183390.1"
                     /translation="MTRRYFGTDGVRGRVGKAPITPDFVLRLGQAAGRVLRRQSLTGH
                     ATVLIGKDTRVSGYMLEAALQAGFSSAGVDIVLCGPIPTPAVAYLTRALRLDAGVVIS
                     ASHNPYDDNGIKFFSGEGTKLPDAVELGIEDELDAGCTCVPSAELGKARRLDDAAGRY
                     IEFCKSTIDFSTDLKGLRILVDCANGAAYHIAPDVFHELGAEVVAVGNTPDGLNINDG
                     VGATHTENLSRLAAEHGCDVAVSLDGDADRLIMADAEGHVYNGDELLYVIVRDRMLQG
                     PVAGVVGTLMTNYALERRFGELGVSFVRAKVGDRYVLEELKRNGWLFGGESSGHILAL
                     DRQTTGDGIVSALQVLSAMRRSGRSLAELTADLQLLPQVLVNKRIPAGYDWQNDRDFS
                     RTVEACRTEVEGRGRVLIRPSGTEPLLRIMVEADDAYLAAELTSRMAESLSVEHQA"
     gene            12967..13308
                     /locus_tag="ACZPB6_02540"
     CDS             12967..13308
                     /locus_tag="ACZPB6_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183391.1"
                     /translation="MAEEVKSADAPVQQPAKGPFVHSLRVYLVGGQQFTLNEVTDSPE
                     VPRNVVAFINAWRAKKDVWHSPNNDVHFGVRVKDVSMYEYRVGQPAAPKAEEEKVEAE
                     VAAEAQAKAEA"
     gene            13466..13804
                     /locus_tag="ACZPB6_02545"
     CDS             13466..13804
                     /locus_tag="ACZPB6_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR1183392.1"
                     /translation="MKLIVAIIKPFKLDEVREALADVGVNGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKLKIEAVVADDILEQAIEAIRKAAYTGKIGDGKIFVYEVEQAIRI
                     RTGEMGEDAV"
     gene            complement(13918..>14429)
                     /locus_tag="ACZPB6_02550"
     CDS             complement(13918..>14429)
                     /locus_tag="ACZPB6_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIR1183393.1"
                     /translation="AMAEFGVRKMSQKKLADTKAPKPRPVALNLGTGEDKEPESQMSD
                     LLDASVFLETEDGRMMHRKGVSPDIPRKLYRGEWPVEAAIDLHGMRVDEAREAVARFI
                     LENHMSGRRCLRIVHGVGYGSKGQGVLRDMVRRWLKQRPEVMAFVQCPPNDGDEGAVR
                     VLVTQQKRD"
BASE COUNT         2755 a         4796 c         4428 g         2450 t
ORIGIN      
        1 cgggcatctc cgactccatc ctcgtcttcg accagcacag aaccctcacg cgcaatcccg
       61 tccatgcggt ccgccgcctc atggcgatgg agcccgagcg aaaggtctgc gtcgaggtcg
      121 acacgcccga ggacggcatg ttctgggctg acaagggcgt gcacatcatc cagtgcgaac
      181 gcttcacgcc cgaggtcctc gcggccttcg tgcgcgaggt gaaggcgaag cacccgcacg
      241 tcgttgtcaa cgccgcaggc ggcatcaatg gcgacaacgt gggtgactac gcggacaccc
      301 gctgcgacgt gctggtcacg agctggccct atttcggcag gcccgctgac atcaagatga
      361 gcttctcgcc cgcgaatccc aagccatgag catccgtctt tccccagccg tcgagagtct
      421 ggtcaaggcc ctcggaaaac ttccgggcta tggcccccgg tcggcccagc gcgccgcctt
      481 ccatctgctt cagaacaggg atgagtccct cgacgaactc gccgccgccg tcgagcgcgt
      541 gcgccgaggc gtcggccgct gccgcctctg caacaccttc accgaagggg atatctgccc
      601 cgtgtgcctt gacgaggaga gggaccctgg acttctctgc gtcgtggaga gcacggcgga
      661 ccagatggcc cttgatgcgt cgctcgcctg gcccggctac tacttcgtcc tttcggggcg
      721 cctgagcccc gtcaacggca tcggacccgc cgacatcggc atgccgttcc tgctcaaacg
      781 cgtgggggcg ggcgtcgagg aaggcgtgct gcgcgaggtc gtcgtcgcca tgagctacac
      841 gcccgagggc gacgcaaccg cctactatct gatcgacgcc ctcaagaagc ggtggccgca
      901 gctgcgcgtc acccgccttg caagaggcct gcccgcaggc atcgaaatcg agtacacgga
      961 cctgagcacg atcgccaacg cggtctacgc cagaagggat gcctgagagg tcgtgcgagg
     1021 cccctcgggc ctccgcccga cggcggcaaa accgagtgca tttgtcgcca aaaacgacca
     1081 ttgttgaacc gttttcggat gaaaaggaat tacttttcct tcgtccgatt tcggacaatg
     1141 cgactagggt tttctgctca gacaaggagc ggaaaagggc tctcccgcct acatagaatg
     1201 acagggttcc caagcccgtt tgtcgctaac ggctcagcta gacaataggg caaaaaaaat
     1261 aagagcctcg agacatccag atgcactctc tcttcaaaga cccgcgcccc gaagccgcac
     1321 tcattctttc ggacggcacg gtactcctcg gacgcagctt cggtgcgagc gtctccaatg
     1381 ttgccgaagt cgtcttcaat acggcgatga ccggctatca ggaggtcctc acggatccgt
     1441 cctatacggg ccagatcgtc acgctcacgg gcgcccacgt cggcaacgtc ggcgtcaacg
     1501 cccaggaccg cgagggcggc gcagccgttc atgccgcagg cctcgtcgtc agggacgttc
     1561 ccgccgtcca ctccaacttc cgctctgaaa agtccctgcc cgactacctc gtcgagcagg
     1621 gcgttcccgc gattgcaggc gtggacacgc gccgcctgac gcgcatcctc cgcacgaagg
     1681 gcgcccaggc cggcgtgatc gtcgtcgccc gcaacggtgc gctcaccgag gacgagatcg
     1741 acgcaggccg caaggccgtt gccgcctgga agggcatggc gggccaggac cttgccaagg
     1801 tcgtcacctg caaggctccc tatgagtgga ccgaaggcgc cttcagcatg aaggccgcgg
     1861 acggcaggcc cggctttgtg acgcccgcag agctccccgt tcacgtcgtc gcctacgact
     1921 tcggcgtgaa gaccaacatt cttcgcatgc tcgtcgaacg cggcatgcgc gtgaccgtgg
     1981 tgcccgcgca gacgcccgtg gaggacgtgc ttgccatgaa ccccgacggc gtcttcctct
     2041 ccaacggccc cggcgacccc gccccctgca cgtatgccat cgaggccgcc cgcgagatca
     2101 tcgcgaggaa gattccgctt ttcggcatct gcctcggcca ccagatcatg gggctcgccg
     2161 tcggcgccaa gacgctcaag atgaagttcg gccatcacgg cgcgaaccat cccgtggtgg
     2221 atttgcgctc gcgacacgtg tacattacga gccagaacca tggttttgcc gtggatgcaa
     2281 aaacgcttcc cgccaacgcc gtgccgacac acgaatcgct cttcgacggt tcgctgcagg
     2341 gctttgagct caccgacgcg ccggccttct gcttccaggg tcaccctgaa gccagtcccg
     2401 gtccgcatga catcgccccg cttttcgacc acttccgcga actgatcgag aggcacagag
     2461 cagcctgacc gtcccctgag ctccgcacag actcaaagag gaccaacatg gaaaaagcaa
     2521 acgttctggc cgcactcaac gacgcacgcg atgcgctcga cgccctgatc tccaacgaga
     2581 agacgattga agccgtcgtg gcggcggcag gcctcatggc ggacgccgtc gagggcgacg
     2641 gcaaggtgat gagctgcggc aacggcggca gtctctgcga cgccatgcat tttgcggagg
     2701 agatgacggg ccgctaccgc agcaaccgtc gtccctacgc agcgcttgcg atttccgacg
     2761 cctcgcacat ggcctgcgtc ggcaacgact acggctacga ggaggtcttc tctcgttatg
     2821 tcgaggcgca cggccgcaag ggcgacgtgc ttcttgccat cacgacgtcc ggcacgagcc
     2881 gcaacatcgt caaggcggcc gaggtcgccc gcaggaaggg cgtcaaggtc gttgcgctca
     2941 cggggcgcga cgagacgccc atcaccgagc ttgccgacgt gtccatcgtg accccggcgg
     3001 gccgctgggc cgaccgcgtg caggagctcc acatcaagtg catccatatt ctcatcgagc
     3061 tcattgaaag gcggctcgac aagcaaaact acgagtaaaa catgcccaag cgtacagaca
     3121 tcaagtccgt tctcatcatc ggtgcgggcc cgatcatcat cggtcaggcc tgcgaattcg
     3181 actactccgg cgcacaggcc tgcaaggccc ttcgcgagga aggctaccga gtcatccttg
     3241 tgaactccaa tccggccacg atcatgaccg acccgcagac cgcggacgtc acctacatcg
     3301 agccgatcac ctgggaggtc gtcgaacgaa tcatcgccaa ggaacgtccc gatgcgatcc
     3361 tgccgacgat gggcggccag acggccctca actgcgcgat ggacctcgcg cgtcacggcg
     3421 tgctcgacaa gtacgacatc gagctcatcg gcgccaagcc cgatgccatc gacaaggccg
     3481 aggaccgcga gcgcttcaag gaagccatgg accgcatcgg acttgagtcc gcacgctcag
     3541 gcgtcgccta ttcccttcgc gaggccaagg agcttcaggc gaagttcggc ttcccgaccg
     3601 tgatccgtcc gtctttcacg ctcggcggct ccggcggcgg catcgcccac gacatggaga
     3661 gcttcatcga gatctgcgaa cgcggcctcg agctctctcc cacgcacgag ctcctcatcg
     3721 aggagtcgct cctcggttgg aaggagtacg aaatggaagt cgtccgcgac actgcggaca
     3781 actgcatcat cgtctgctcc atcgagaact tcgacccgat gggcgtgcat acgggcgact
     3841 ccatcacggt tgcgcccgcc cagacgctca ccgaccgcga gtaccagcgc atgcgcgacg
     3901 cctcgatcgc cattctgcgc gagatcggcg tcgacacggg cggctccaac gtccagttcg
     3961 ccgtcaatcc cgagacgggc cgcatgatcg tcatcgagat gaatccgcgc gtctcccgct
     4021 cctccgcact cgcctccaag gccacgggct tcccgatcgc tcgcatcgcc gccaagctcg
     4081 ccgtgggtta cacgctcgac gagctgcgca acgaaatcac cggcggcctc acgcccgcct
     4141 ccttcgagcc gacgatcgac tacgtcgtca cgaaggtgcc gcgcttcgcg ttcgagaagt
     4201 tccctgccgc caacgaccgc ctcacgacgc agatgaagtc cgtgggcgag gtgatggcca
     4261 tcggccgcac cttccaggaa tccctgcaga aggccctgcg cggtctggag accggccaca
     4321 acggcctcaa cgaaacgggc gtcggcatcg agcagcttcg ccacgaaatc gccgtgcccg
     4381 gccccgaacg catcttcttc cttgccgatg gcatccgcgc cggcatgacg gacaaagagc
     4441 tccacgaact ctccgccatc gatccgtggt tcctcaatca ggtccgcgag atcgtcgaga
     4501 ccgaggacaa gctccgcggc aatccgttct ccgcgttcac gaagtccgac atgctcttcc
     4561 tcaagcgcaa gggcttctcc gacggccgcc ttgcccagct gacaggcgcg aaggaggccg
     4621 aggtccgcgc cgcccgcctg gcgatgggca tccgtcccgt ctacaagcgc gtcgacacct
     4681 gcgccgccga attcgcgacc aacaccgcct acctctactc cacctacgag gaggagtgcg
     4741 aggccgaacc caccgaccgc aggaaggtca tggtgctcgg cggcggtccg aaccgcatcg
     4801 gccagggcat cgagttcgac tactgctgcg tccacgcctc gaaggccctg cgtgccgacg
     4861 gctgggagac gatcatggtc aactgcaatc cggaaacggt gtcgaccgac tttgatacgt
     4921 ccgaccgcct ctacttcgag cccgtgacgc tcgaagacgt gctcgccatc tgcgaagtcg
     4981 agaagcccgc aggcgtcatc gtccagtatg gcggccagac gccgctcaag ctttgccgcg
     5041 ccctcgaggc cgcaggcgtg cccatcatcg gcacgacgcc cgacgccatc gactgcgccg
     5101 aggatcgcga gcgcttccag cacctgcttc agaagctcaa ccttcgccag ccgcccaacc
     5161 gcacggcccg gaccgaggag gacgccatca ggctcgccgc cgaaatcggc tatccgctcg
     5221 tcgtgcgtcc gtcctacgtg ctcggcggcc gcgccatgga catcgtgcac acgcccgaag
     5281 ccctgcagcg ctacatgaag gaggccgtgt cggtctccaa cgaaagcccc gtacttctcg
     5341 accgcttcct tgacgacgct gttgaaatgg acgtcgacgc cgtggccgac ggtcatgagg
     5401 tccaggtcgc cggcgtgatg gaacacatcg aacaggccgg cgtgcactcg ggcgactccg
     5461 cctgctcgct gccgccctac agccttcccg ccgtcgtggt cgaggagatc aagcgccaga
     5521 cgaaactcat ggccgaggcg ctcaacgtgg tgggcctcat gaacgtgcag ttcgccgtcc
     5581 agcacgcgca ctccgaaaac cccgtcatct acgtccttga agtgaatccg cgcgcctccc
     5641 gcacggtgcc gttcgtctcc aaggccacgg gcaaccccgt cgccgccatc gccgcacgcg
     5701 taatggcggg ccagacgctc gccgaacagg gcgtgaacct cgaggtgacg ccgcgctacg
     5761 tgagcgtcaa ggaggccgtc ttcccgttct ccaagttcct cggcgtcgac ccggtcctcg
     5821 ggccggaaat gcgctcgacg ggcgaagtga tgggcgtggg ccgtgacttc ggcgaggcgc
     5881 tcttcaagag ccagctcgcc gcaggctcca ggctccccga acgcggctcc gtgttcatct
     5941 cggtgcgcga gtgcgacaag cccaaggccg tcgtctgcgc ccaccagctc caccaggcgg
     6001 gcttcccgct cgtggcgacc gcgggtactg ctcacgtcat ccagcaggcc ggcatcccgt
     6061 gccgcaccgt gggccgcatc ggcgacgctg cgggcgacgt gatcggcatg atggaggcgg
     6121 gcgacatcac gctcgtcatc atgtcggtgg ccgagcacga gggcgagctc aacgatgcgc
     6181 gcgccatccg caagcttgcg ctggccaagc agatcacgta ctacacgacg atggcgggcg
     6241 gtcttgccgc ctccgagggc atccgccaca tgcgctccgt gcaggtctac gaccttcagg
     6301 gcctgcatgc gggcacgctg ccctgacgga tttgctaaac taacgtagca cagactgggc
     6361 cgcaacgcgg tccggtcgtc cggattttcg atgacgtccg gcgagcttcg tctcgtcgga
     6421 cgtttccatt cattcagagt ttcctttttc gagaattagc aacatggtct cgacccgcat
     6481 tcccatcacg acccgaggcg ccgaaaagct gaaggccgaa ctcgactacc tcaagaaggt
     6541 cgcccgcccg aacgtggtgg ccgccatcgc cgaagcccgt gaaaagggcg acctttccga
     6601 aaacgcggag tacgatgccg cccgtgaaga gcagggtcac attgaaggcc gcatcgccga
     6661 gctcgaaggc aagattcccg ccctgcagat catcgatccg aagagcgtcg acgccggcga
     6721 gcgcatcgtc ttcggcgcca cggtcggcct ctacgacgag gatgacgacg tgagcgtcac
     6781 ctaccagatc gtcggcgacg acgaggcgga catcaaggaa aaccgcattt cagtctcctc
     6841 tccgatcgcc cgcgccctga tcggcaagta cgagggcgac agcgtccagt tcatggcccc
     6901 gaagggactc gtctcctacg aaatcgaatc cgtcgagtac atctgatccg gattcacgaa
     6961 gccctgaatc aaaaaaagag cgcagccgtc aagactgcgc tcttttgttc ggtgccctta
     7021 agtcagggag atcaggagcg ggagcccgca cccttcttcg tcacacggtt cttcttggaa
     7081 cgggagcgga cgggaatcgg ggaggtggcc ttcttctttt cgcccggctt cttggaggcg
     7141 accttcttgg cgggcttcgc gacggccttg agcggcgtgc gcttggcagg ggccgacgga
     7201 gcctccttct tgccggcaac ggaggccgtc ttcatggaga ggatggcgag gcgggcggct
     7261 tcatcgggat cctgacgctc gctctcgtcg gcgggacgcc agaagatgag aagcttgccg
     7321 atcatctgga cgcgggcggc gccgagcttt tcggcgactt cggcgtagat ggactcaagt
     7381 tcctcgcggt cgtcggacgg aacgcggacc ttgatgagct cgtggtcgtt gagggcgcgg
     7441 tcgatttcgt gaagaacggc gttggtgagg ccgttggcgc cgagaaggac gaccgggtca
     7501 agatggtggg cctgggaacg aagggccaga cgcgcatcgc gcgcgagaat gatttctttc
     7561 ataataaacg gatgtgtccc cgcggcgctt caaagggaag cgctcgaagc ggggggctga
     7621 taaacaaagt tggctgggga aaatatgcct gtagcaacca aaaaactgcg ctccaaccgc
     7681 gcctggatcg aacgtcacat caatgatccg ttcgtgaagc gttccaaggc cgaaggatac
     7741 cgagcccgct cggtctacaa actgaccgag ctcgacgatc gtgaacatct gcttcgccgc
     7801 ggcatgaccg tggttgaact cggcgcggcg ccgggaagct ggacgcagat cgtccgcgag
     7861 cgtttgtcgg acaaggacgg ccgtgtccag ggccgcatca tagccatgga tatcctgccg
     7921 atggatccga tcgacggcgt gacattcctg cagggagatt tccgtgagca ggaaatcgcg
     7981 gacaaattag cagaaattct tgacggcgac aaggtcgacg tcgttttaag tgacatggcc
     8041 ccgaatcttt cgggcatcgc cgctgcggac gccgcccgtt cgctgcttct caacgagctc
     8101 gcgcacgagt tctgtctcga gcatctcaag cccaacggcg tcttcgtgac aaagtcgttc
     8161 cagggttcgg gcttctcgca gttcgtcgag gcgctcaaga agaccttcaa gaaggtttac
     8221 acgcgcaagc ccgccgcctc tcgcgacacg tcggccgaag tctacctcgt cgcccgcgag
     8281 ctcaagcccg gcaagtagtc cctgcggccg aaataatcgg tcacgatttt tcggctcccc
     8341 gcacaaatag gtgcgagcaa atgcgcgtaa gggggcgaca ttcgcgcaga acgagcggct
     8401 aaaatatgcc cattacgcct ttgccgatgc cgaaaggctt tcgaggcgaa ggccttctta
     8461 caagactaag gaagatgacg gcgcagggcc cgactgcggc tccgccgtca ggagacatac
     8521 attggacaac aaaatcttca gccgcatcgc cgtctgggcg ctggtcggtc tggtgctctt
     8581 cacggtgttc cgccagtatg aagacgccta cgccccgccg cgcgacacga tcagctacac
     8641 gcagttcatg gaggacgcca aggcgggcaa ggtccgtcgc gtcgacatcc agggccgtac
     8701 gctcatcgtc acgccccaga ccggcgagga attcaagctc accaatccgg gcgacatctg
     8761 gatggtggac gagctcagga agagcaacgt tcagatctac ggcaaggccg aggaggagcc
     8821 gtctcttctg acgaccatct tcatctcctg gttcccgatg atccttctca tcggcgtgtg
     8881 gatcttcttc atgcgccgca tgcagggcgg tgcgggcggc ggtggcggag cgttcagctt
     8941 cggcaagtcg aaggcccgca agcttgacga ggaaagcaac aaggtgcgct tccgcgacgt
     9001 cgcgggctgc aacgaggcca aggaagacgt tcaggaaatc gtggacttcc ttcgcgaacc
     9061 caagcgcttc cagcgtctcg gcggccgcat tccgcgcggc ctgctgctcg tcggacctcc
     9121 gggcaccggc aagacgctcc tcgccaaggc gatcgccggc gaggcgggcg tgccgttctt
     9181 cacgatctcc ggttctgact tcgttgaaat gttcgtcggc gtgggcgcag cccgcgtgcg
     9241 cgacatgttc gagaccgcca agaagaacga gccctgcatc atcttcattg acgaaatcga
     9301 tgcggtcggc cgccaacgcg gcgcaggcct cggcggcggc aatgacgaac gcgagcagac
     9361 cctcaaccag atgctcgtcg agatggacgg cttcgactcc ggcgccaacg tgatcgtgat
     9421 cgccgcgacg aaccgtcccg acgttctcga ccccgctctg ctgcgtccgg gccgcttcga
     9481 ccgccaggtg gtggtgccgc tgcccgacat ccgcggccgc gaccagatcc tcaacgtgca
     9541 catgcgcaag attcccgtcg gcaaggacgt cgacagcatg gtgatcgcgc gcggtacgcc
     9601 gggcttctcg ggcgccgatc ttgcgaacct cgtcaacgag gccgcgctct tcgccgcccg
     9661 ccgcaacggc cgcgtcgtga agatgtgcga ctttgaaaac gccaaggaca agatcatgat
     9721 gggcgccgaa cgccgcgcca tggtcatgag cgaggacgag cgccgcaaca cggcctacca
     9781 cgagtccggc catgccgtcg tggcacgcct gatgcccaag tccgatccag tccacaaggt
     9841 cacgatcgtt ccgcgcggtc gtgcgctcgg tctgacgatg cagcttccga aggaagaccg
     9901 ctacgcctac gatcgccagt acctgctcac gcgcatcgcg attctcttcg gcggccgcat
     9961 cgccgaggag gtcttcatga accagatgac gaccggcgct tcgaacgact tcgagcgcgc
    10021 cacgcagatg gcccgcgaca tggtgatgcg ctacggcatg agcgacaggc tcgggcccat
    10081 ggtctactct gaaaacgagg gcgaggtgtt cctcggccgc tccgtgacga agacgaccaa
    10141 catctccgag aagacgatgc aggaagtcga cgccgaagtc cgccgcatca tcgacgagca
    10201 gtacgccatc gcgcgcaggc tcatcgagga aaaccgcgac aagatggaag tcatggccaa
    10261 ggcgcttctc gactgggaga cgatcgacgc cgaccagatc gacgacatca tggaaggccg
    10321 cgcgccgcgt ccgcccaaga cctccaagga ggccgtgaag cctgaagcgg cgcaggccgc
    10381 cgcgcccgag ggcgaggcag gcgccgcaga agagcccaag gccgaagatg ccgaggctcc
    10441 cgcggctccc gaagccgccg gcgcgaagga ggcttcctcc tccgacaatg ccggtgcccc
    10501 gaagggcgat gatgaccgcc gctccgaata acctcggaac aggctaagat aggctgcagg
    10561 gctgaagaga ggccctgcag cctttttcaa ttgataaaaa caaaagcgat aaaagcaaga
    10621 tgacagaact tcacgagcgc gccagcgtct ggcgctgtgg cgccaaggtc ttcgagatcg
    10681 accgcctcca cccgctcatc atgggcattc tgaacgtgac gccggactcc ttttccgacg
    10741 gcggcaccca caacggcctt gaggacgccg tcgcctgggc gcaccgcatg cgcgacgaag
    10801 gcgcgcacat catcgacgtg ggcggcgagt ccacgcgtcc gggcttcaac gaaaccggcg
    10861 tcacgcttga agaggagcgc cgccgcgtca ttcccgtggt cgagcaactc gtgaaggaag
    10921 gattcatcgt gagcgtcgac acctccaagc cggagatcat gaccgaggtg gcttcgctcg
    10981 gcgcgcacat cctcaacgac atccgcggct tcgagatgcc cggcgcgctt gaagccgccg
    11041 cggcgacgga ctgcggcctc gtcgtcatgc accgctcggc caccacggac tacaaggatg
    11101 tcgtggcgga ggtcgaggcc taccttcagg aaaggcagcg cctcctcgag gggctcggcg
    11161 tttcggcgga ccgcatctgc tgggatccgg gcttcggctt cggcaagacc gtcgagcaga
    11221 acttcgcgct gctcggctcc acggaaagat tcgttgcatc cggtcagccc tatctgatgg
    11281 ggctctctcg caagtcgtcc atcggggcgg ccacgggcca caaggcgccc atggaccgca
    11341 ttgcgggcag catcgccggc gcgcttatcg ccgtcgagca gggcgcccag gtcgtccgcg
    11401 tgcatgactg ccgccagacc gtcgacgccg tcgccgtctg gcgcgcgtcc gaggaggcct
    11461 gcggacgcag gtaagaaaca acctaaggag aacaatcatg actcgtcgtt attttggtac
    11521 ggacggtgtt cgaggccgtg tcggcaaggc gccgatcacg cccgacttcg tgctgcgcct
    11581 cggtcaggcc gcaggccgcg ttcttcgccg ccagagcctg acgggacacg cgaccgtgct
    11641 catcggcaag gacacgcgcg tttcgggcta catgctcgag gctgcgcttc aggcaggctt
    11701 ctcgagcgcc ggcgtggaca tcgtgctctg cggtccgatt ccgacgcccg ccgtcgccta
    11761 tctcacccgc gcccttcgcc tcgacgcggg cgtggtgatc tccgcgagcc acaatccgta
    11821 cgacgacaac ggcatcaagt tcttctcagg cgaaggtacg aagctgcccg acgccgtcga
    11881 gctcggcatc gaggacgagc tcgacgcggg ctgcacctgc gtgccttcgg ccgaactcgg
    11941 caaggcccgc cgccttgacg atgcggccgg ccgctacatc gagttctgca agtcgacgat
    12001 cgatttcagc acggacctca agggcctgcg cattctcgtc gactgcgcca acggcgccgc
    12061 ctatcacatt gcgcccgacg tcttccacga actcggcgcc gaagtggtcg ccgtgggcaa
    12121 cactccagac ggcctcaaca tcaacgacgg tgtgggcgcg acccacaccg agaacctctc
    12181 gcgccttgcc gccgagcacg gctgcgacgt cgcggtgagc ctcgacggcg acgcggaccg
    12241 cctgatcatg gcggacgccg aaggccacgt ctacaacggt gacgaactcc tctacgtgat
    12301 cgtgcgcgac cgcatgttgc agggcccagt cgcgggcgtt gtcggcacgc tgatgaccaa
    12361 ctacgccctc gagcgccgct tcggcgaact cggcgtctcc ttcgtgcgcg ccaaggtcgg
    12421 cgaccgctac gtgctcgagg agctcaagcg caacggctgg cttttcggcg gcgaatcgtc
    12481 gggccacatt cttgcgctcg accgtcagac gacgggcgac ggcatcgtga gcgcccttca
    12541 ggtgctctcc gccatgcgcc gcagcggcag gagccttgcc gaactgacgg ccgacctgca
    12601 gcttctgcct caggtactcg tcaacaagcg cattcctgcc ggctacgact ggcagaacga
    12661 ccgtgacttc tcgcgcacgg tcgaggcctg ccgcaccgag gtggagggcc gcggccgcgt
    12721 gctcatccgt ccgtcgggca cggagcccct gctgcgaatc atggtcgagg ccgacgatgc
    12781 gtatcttgcc gccgaactga cttcccgcat ggccgagagc ctttccgtcg agcatcaggc
    12841 ttgatctacg acgaaacatt ggtaaattca cggtgacacg acagagtcaa acttgctaaa
    12901 ctgcaggata cgattccatc ccgaggatgg tgttcaagag tttttagatt gataaggaag
    12961 agataaatgg ccgaagaagt caagtctgcc gatgctcccg ttcagcagcc cgccaagggt
    13021 ccgttcgtgc attccctgcg cgtctacctc gttggcggcc agcagttcac cctcaatgaa
    13081 gtgacggatt cccccgaagt tccgaggaac gtcgtcgcct ttatcaacgc ctggcgcgcc
    13141 aagaaggacg tctggcacag ccccaacaac gacgtgcatt tcggcgtccg cgtgaaggat
    13201 gtgtccatgt acgaataccg cgtcggccaa ccggctgctc cgaaggctga agaggaaaag
    13261 gttgaagccg aggtcgctgc cgaggctcag gccaaggctg aagcctgata tcgggtcatc
    13321 cgctgaagaa agccggactg ttttcgggca gttcggcttt tttgtcttca aaaaaacggt
    13381 tcgggcgctc gcaagcgggt ctgcatttga tagacttccg agcatctgtc tttttattcc
    13441 cggcccttgt gccggtgcat gtgtcatgaa gctgattgtt gccatcatca agccgttcaa
    13501 gctcgatgaa gttcgtgagg cactcgccga cgtcggcgtg aacggcctta ccgtcacgga
    13561 agtgaagggc tttggccgcc agaagggtca caccgagctc taccgcggtg ccgaatacgt
    13621 cgtggacttc ctgcccaagc tcaagatcga ggccgtcgtc gccgacgaca ttctcgagca
    13681 ggccatcgag gccattcgca aggccgccta caccggcaag atcggcgacg gcaagatctt
    13741 cgtctacgag gtcgagcagg cgatccgcat ccgtacgggc gaaatgggcg aggacgccgt
    13801 ctgacaggca tcccgcgtgc gcgccttcga ggcctgcagg atgaaattga aaggcagagg
    13861 ggacaggcat gagcctgtcc cctctgcttt tattgccaga agatcccgag tccttcctca
    13921 gtcgcgcttc tgctgcgtga cgagcacccg cacggcgcct tcgtcgccgt cgttgggcgg
    13981 acactggacg aaggccatca cctcgggacg ctgcttgagc cagcggcgga ccatgtcgcg
    14041 cagaacgccc tggcccttgc tgccgtagcc cacgccgtga acgatgcgca ggcagcggcg
    14101 cccgctcatg tggttctcga ggatgaagcg ggcgaccgct tcgcgagcct cgtcgacgcg
    14161 catgccgtgg aggtcgatcg ccgcctcgac cggccattcg ccgcgataga gcttgcgggg
    14221 aatgtcgggc gagacgccct tgcggtgcat catgcggccg tcttcggttt caagaaagac
    14281 ggaggcgtcg agaaggtccg acatctgcga ctcgggttcc ttgtcctcgc ccgtaccgag
    14341 attgagggcg acgggacggg gcttcggagc cttggtgtcg gcaagcttct tctgggacat
    14401 cttgcggacg ccgaactccg ccatggcgg
//
LOCUS       DBMDTI010000025        14163 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1338_length_14163_cov_2.978453, whole genome shotgun sequence.
ACCESSION   DBMDTI010000025 DBMDTI010000000
VERSION     DBMDTI010000025.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 14163)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14163)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14163
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1338_length_14163_cov_2.978453"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="ACZPB6_02555"
     CDS             <1..859
                     /locus_tag="ACZPB6_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1183394.1"
                     /translation="VTYLDVKEDGLIDMDAFRAAIRPDTILVSVMAINNEIGVIQDLE
                     TISAICHEHGIIFHCDATQAAGKMDLNVDRLKVDLMSLSGHKVYGPKGIGALYVRRKP
                     RVRIECQIHGGGHERGMRSGTLATHQIVGMGEAYRLAKEEMETEMPRLARLRDRLWDG
                     IRNNIPEVYLNGSMEHRSPHNLNVSFAFIEGESLMMAMKDIAVSSGSACTSASLEPSY
                     VLRALGRDDELAHSSIRFTLGRFTTEEEIDYVIKTLVEGVTRLREMSPLWEMHQQGVD
                     LNQVQWSEH"
     gene            933..1319
                     /gene="iscU"
                     /locus_tag="ACZPB6_02560"
     CDS             933..1319
                     /gene="iscU"
                     /locus_tag="ACZPB6_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HIR1183395.1"
                     /translation="MAYSEKLMDHYEHPRNVGTLDVKDDNVGTGMVGAPACGDVMRLQ
                     IKVNDEGVIEDAKFKTYGCGSAIASSSLVTEWVKGKTLDEAGQLTNAAIAEELALPPV
                     KIHCSILAEDAIKAAINDYRNKQSKE"
     gene            1326..1649
                     /gene="iscA"
                     /locus_tag="ACZPB6_02565"
     CDS             1326..1649
                     /gene="iscA"
                     /locus_tag="ACZPB6_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HIR1183396.1"
                     /translation="MAVTLTEAAAKHVQKFLTRRGKGVGLRLGVKTSGCSGMAYKLEF
                     ADEVNSDDQVWESFGVKVIVDEKSLPYLDGTELDYTREGLQEGFKFHNPNEKSRCGCG
                     ESFHV"
     gene            1728..2228
                     /gene="hscB"
                     /locus_tag="ACZPB6_02570"
     CDS             1728..2228
                     /gene="hscB"
                     /locus_tag="ACZPB6_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HIR1183397.1"
                     /translation="MAQMSPFQLFGMKPAFMIDEAKLEAAYQAAMMKVHPDRFADRPA
                     AERRVAEQWSTRINEAFKLLKSPVKRAAWLCENAGRPIEAETNTRMPADFLMTQIMWR
                     EALESGDAGAADAVRAEAGGVRADVVRGLAEAIDEKADWAAAVDLTRRLMFIDRFLEE
                     AARDSK"
     gene            2246..4204
                     /gene="hscA"
                     /locus_tag="ACZPB6_02575"
     CDS             2246..4204
                     /gene="hscA"
                     /locus_tag="ACZPB6_02575"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HIR1183398.1"
                     /translation="MALFQIAEPGLSAAPHEHRLAVGIDLGTTNSLVASVISGSTEVI
                     LDEGGAAILPSVVHYAADGTVTVGAVALRDASRDPKNTISSAKRLIGRTMKDLADVHN
                     LPYDLVDDPSMVRIATSAGVKSPVEVSAEILRSLAKRAEDRLGGDLTGAVITVPAYFD
                     DAQRQATKDAARLANLTVLRLLNEPTAAALAYGLDNGAEGTYMVYDLGGGTFDVSLLK
                     LTRGVFEVLATGGNSALGGDDFDHRVLCHVTESFRGGNPKARFTVDGKPADTLPGHIL
                     SAAEKRRLMALSRAAKEALTKDESTCIRFAFDNGDEVELDLTREEFDAMTCHLVRKTI
                     DTVEAVLRDASAGVSDVRGVVLVGGATRMPCVRTAVEKFFGFAPLDDIDPDKVVAIGA
                     AMQANKLAGNTSGDDDWLLLDVTPLSLGLETMGGLVEKVIPRNSAIPVALAQDFTTFR
                     DGQTAMAIHVVQGERELVDACRSLARFELRGIPPMAAGAARIRVTFQVDADGLLSVSA
                     REMTTGVEASIQVKPSYGLSDDDIVRMLQDGNGSAEEDMRARELREQQVEARRLLEST
                     HSALASDGDLLDDAERAEVDALVERLTALVISDDVEAVKAGIEALTKGTETLAERRMD
                     RSIREALSGMSVDDEMFDEKPEDESKNH"
     gene            4241..4576
                     /gene="fdx"
                     /locus_tag="ACZPB6_02580"
     CDS             4241..4576
                     /gene="fdx"
                     /locus_tag="ACZPB6_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HIR1183399.1"
                     /translation="MPKVTVLPHETLCPEGLVFEAATGENLARALLAHGVKIEHACEF
                     SCACATCHVIIREGFDSLEEPDDNELDHLDTAWGASVLSRLSCQTKVGEGDITVEIPK
                     YSRNHAREE"
     gene            4580..4780
                     /gene="iscX"
                     /locus_tag="ACZPB6_02585"
     CDS             4580..4780
                     /gene="iscX"
                     /locus_tag="ACZPB6_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HIR1183400.1"
                     /translation="MSLKWTDAQAIAEELYDADPDLDPLTLSFVKLHDMICALPDFDD
                     DPEASNEKILEGILMKWLDERD"
     gene            complement(4869..5237)
                     /locus_tag="ACZPB6_02590"
     CDS             complement(4869..5237)
                     /locus_tag="ACZPB6_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183401.1"
                     /translation="MQILIVTPEAARWTAFGEGLRAAGLSCSFAGAPDDLKAESLKAD
                     RPLLVLWDEPYTGDALRAAGIGVIMADARINQSACTDEGHETFHKKVEGLGFLPNVPL
                     TRTAEDARALADSLVKVSAL"
     gene            complement(5251..5622)
                     /locus_tag="ACZPB6_02595"
     CDS             complement(5251..5622)
                     /locus_tag="ACZPB6_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HIR1183402.1"
                     /translation="MKIAIPSVLPGGLEAQVGAHFGHCDCYTLVDVEDGAVVKSEVIP
                     SCAHEHGGCLAPVNYLADRGVTVLLAGGMGMRPLMGFLQEGVDVYYAAGIETVKKAVA
                     AHIAGELPRFSPNHSCGGGHH"
     gene            complement(5619..6542)
                     /locus_tag="ACZPB6_02600"
     CDS             complement(5619..6542)
                     /locus_tag="ACZPB6_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1183403.1"
                     /translation="MHEIVVLSGKGGAGKTSVTAALARAMSAREKIVVCDYDVDAPDL
                     HILLKPETEMRADFVSGHLAVFNSDNCIDCAQCLAFCRFDAIKQAAPCEYPVIEQLCE
                     GCAVCVKLCPELAFDFVPRHCGEYYRSRTRFGTMIHAELTPGAENSGRLILLLKQEAA
                     KAAQADGAEIILSDGTPGIGCPVISSLSGAGLVCAVIEAGASGIADFKRLAELARRFR
                     LPIAVIVNKADLSAEATEAAMQAARDAGAEVLGTLPFTPVFTEAMSRGEAVGETPSEL
                     DGHFQNFADRLLALVRGGRRSRIIPIKEKAS"
     gene            complement(6535..7419)
                     /locus_tag="ACZPB6_02605"
     CDS             complement(6535..7419)
                     /locus_tag="ACZPB6_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HIR1183404.1"
                     /translation="MRILFASGKGGAGKTTVTAGIAAFWPNPAVLADADVEAPDLGLY
                     LPHAFAEETPVGLEVPVRVTDACDLCGECTSICQFNAIKKLGGRIMPFSDMCHGCGGC
                     TAVCAPHAIVTGERELGRIGFGALEDGRIFLEGRSRVGEAMTPPLIRALLSKASACAA
                     REKADLLIDAPPGVSCPVMTAAASADAVVLVIDPTPFGVHDFKLALRAFESLGKPMAA
                     VINRSGQPSAAACEAELIAFCGEKGLPVAARLPFDREAAARAAAGRHPMLDSEDWREC
                     LSEAARTLSTLFEGGRHA"
     gene            complement(7419..8102)
                     /locus_tag="ACZPB6_02610"
     CDS             complement(7419..8102)
                     /locus_tag="ACZPB6_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014620.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HIR1183405.1"
                     /translation="MSRPPRSRIVDMPMRTVYFKPSGKLPASAERVSLKFEELEALRL
                     ADLEGLSQSEASSGMGVSRHTFGRVLASARKKVALSLVNNIPLVVTGGTNSCRRESGE
                     LLKEHPAMKIAISAEGPTLEDDVDPRFGRAAGFIIYDTETKAVEYIENGAGQTAAQGA
                     GLIAVETVSNAGATVALSGYIGPKAFDALQAIGIGTVQDVDNRTVGEVVRAFEAGELT
                     VILPSNKEA"
     gene            complement(8255..9073)
                     /locus_tag="ACZPB6_02615"
     CDS             complement(8255..9073)
                     /locus_tag="ACZPB6_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012514.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR1183406.1"
                     /translation="MDRLDSIRRIRAAELLAELRTRMQTESPESLGAFVARGLELGSL
                     REDVMHALRTDPELRALAPEVRPRVFELFAPEGLEPAFAALTAGLAHHGLTQKRPFEL
                     LDVRDARGRIVARAERSVFRALGLATVVVRLLALAPDGRLLLQQRSLSKSIGPGLWDN
                     LAAGLVSSGETPAEAMLRELHEEAGLDPEFSRLHSPAHLSWQVLHDVPEGRMQESTAG
                     FILRLSPCDAPVNLDGEAAGFAVFNTEEVLSMIEEGRIMPEAARLILEHLLHAD"
     gene            complement(9081..10610)
                     /gene="lysS"
                     /locus_tag="ACZPB6_02620"
     CDS             complement(9081..10610)
                     /gene="lysS"
                     /locus_tag="ACZPB6_02620"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR1183407.1"
                     /translation="MSEQTQNQETAQQLAEEENHIIAERRAKLAALREAGVAYPNDFV
                     RTDLFGDIRAKYQDATAEELEELKPEVACSGRMMLKRIMGKASFATVRDFTGDMQYFI
                     TKGDIGEEAYAAWKTFDLGDIVAVKGTLFRTKTGELSIRAHELRLMTKNIRPLPDKHK
                     GLVDTEMCYRQRYVDLIMNEDSRNRFLVRSKAIAAIRSFMLAHRFLEVETPMLHPIPG
                     GANAKPFITHHNALDMDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGVSVRHNPE
                     FTMMEFYCTYWTYRDQMQFTEDLLRTAAREATGSAILPYQGHEIDLEKPFDRLTPVEA
                     ILKYSPDYTAEQLADEAFLRAELVRLGEPQPDYVGLGALQMALFEETTEAKLIQPTFI
                     IDYPTEISPLARASDANHDVTERFELFIAGRELGNGFSELNDPDDQAARFKAQADAKS
                     HGDDEAMYYDADYIRALEYGLPPTAGCGVGIDRFIMLLTDAPTIRDVLLFPHMRPESG
                     N"
     gene            complement(10677..11790)
                     /gene="prfB"
                     /locus_tag="ACZPB6_02625"
     CDS             complement(join(10677..11708,11710..11790))
                     /gene="prfB"
                     /locus_tag="ACZPB6_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143183068.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR1183408.1"
                     /translation="MEVERINQIQNLTDDLTRRLTELRGYLDVDRKARRLEEVNREVE
                     NPDLWNDPKHAQEVSKEKKLLDDIVGSFNKLTSGINDAAELFELSMAEEDWDTVEAVG
                     QDVEEIEKEVAQLEFKRMFNQPMDSANCYLEIQAGAGGTEAQDWASMLERMYMRYAER
                     KGFKVTLEEESPGEVAGIKSCTLYIEGEWAYGTLRTETGIHRLVRKSPFDSNARRHTS
                     FTSVYVYPEVDDSIEIEINPADLSVDVFRASGAGGQHIQKTESAVRIHHIPTGIITIC
                     QDDRSQHRNREKAMQQLKAKLYELEMRKRMEEQTKLEESKSDIGWGHQIRSYVLDQSR
                     VKDLRTNVETGNTGAVLDGDLDQFIEASLKNGVAAA"
     gene            complement(11960..13657)
                     /gene="recJ"
                     /locus_tag="ACZPB6_02630"
     CDS             complement(11960..13657)
                     /gene="recJ"
                     /locus_tag="ACZPB6_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR1183409.1"
                     /translation="MTRILNREFDLEAARRLHEEGYLPPVARALSARGIRDASELAQD
                     WKSMLPPAMLEGTREAAERLALARERGERVTVVADYDCDGATACAVAIRGLGMMGVKA
                     DYFVPHRVHHGYGLSCAVVDLLAARTPRPDVLLTVDNGVSSAEAVRHAAELGIDVIVT
                     DHHLPGAELPPAVCIVNPNLADSAFPSKALAGVGVMYYVLLALRALLRERGVYTQQTQ
                     PRLDALVDLVALGTVADVVKLDKNNRILVAQGLNRIRLGRTHAGISALFAVAGKKPEA
                     AGVRDFGFALGPRINAAGRLDTMENGIECLLADDPVVALDYARSLNDFNAARQELETE
                     MQQAAETALSCSNVDSLATLTLYDGRFNEGVVGLVASRLKEKVNRPTIVFAPTDDGAL
                     KGSGRSIAGVHLRDALDLVSKALPGAVLRFGGHAMAAGLTLRSEDDLPAFRDAFEKAV
                     RSMVDASVFERVIYTDGGLAPDEITERLVQAIDSQIWGQGFDAPVFANEFRVVRQSLV
                     KDAHTKLILELGGQRFDAIFFRHTETLPGMVRLAYRPNINEFMGRRSVQLVIEAAEL"
     gene            complement(13654..>14163)
                     /locus_tag="ACZPB6_02635"
     CDS             complement(13654..>14163)
                     /locus_tag="ACZPB6_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183410.1"
                     /translation="ILADHPVNAARRAAGRHAVDALWISGGGHEELFFPPTLIRSVAS
                     DDAAVRGWANAAGILIERIGRDTGRWPEAPHGDVVAVIEDLYPAWLARDWEAWARALP
                     EVARKAASYREDAKRFEADDVLVVAFGETGAATLAPAEAGLMERLFRRKSSVEPAAWC
                     PDRYEGAVQ"
BASE COUNT         2424 a         4350 c         4908 g         2481 t
ORIGIN      
        1 ggtgacctac ctcgacgtca aggaggacgg cctcatcgac atggacgcct tccgtgcggc
       61 catccgtccc gacacgatcc tcgtctccgt gatggcgatc aacaacgaga tcggcgtcat
      121 tcaggacctc gagacgatca gcgcgatctg ccacgagcac ggcatcatct tccactgcga
      181 cgccacgcag gccgccggca agatggacct caacgtcgac cgcctcaagg tcgacctgat
      241 gagcctctcc ggccacaagg tctacggccc gaagggcatc ggcgccctct acgtgcgccg
      301 caagccccgg gtgcgcatcg aatgccagat tcacggcggc ggccatgagc gcggcatgcg
      361 ctcgggcact cttgccaccc accagatcgt cggcatgggc gaggcctatc gtcttgccaa
      421 ggaggagatg gagacggaga tgccgcgtct tgcgcgcctt cgcgaccgcc tctgggacgg
      481 catccgcaac aacattcccg aggtctacct caacggcagc atggagcacc gctccccgca
      541 caacctcaac gtgagcttcg cgttcattga aggcgaaagc ctgatgatgg ccatgaagga
      601 catcgccgtc tcgtccggct ccgcctgcac gtccgcctcg ctcgagccct cctacgtgct
      661 ccgagcgctc ggccgcgacg atgagctcgc ccacagctcg atccgcttca cgctcggccg
      721 cttcacgacc gaggaggaga tcgactacgt gatcaaaacc ctcgtcgaag gcgtcacccg
      781 cctgcgcgag atgagcccgc tctgggaaat gcaccagcag ggcgtcgacc tcaatcaggt
      841 tcagtggtcc gaacactgat cccacggaac ccgcacagaa cacaacactg accggagagg
      901 cgcgcgagcg ccctccgacg gagatacaag acatggcata cagcgaaaaa ctcatggacc
      961 actacgagca tccgcgcaac gtcggcacgc tcgacgtcaa ggatgacaat gtcggcaccg
     1021 gcatggtcgg cgcccccgcc tgcggcgacg tgatgcgcct tcagatcaag gtgaacgacg
     1081 agggcgtgat tgaggacgcc aagttcaaga cctacggttg cggctccgcc atcgcctcct
     1141 cctctctcgt gacagagtgg gtcaagggca agacgctcga cgaggccggc cagctcacca
     1201 acgccgccat cgccgaagaa ctcgcgcttc cgcccgtgaa gatccactgc tcgatcctcg
     1261 ccgaggacgc aatcaaggcg gccatcaacg actaccgcaa caagcagtcc aaggagtgat
     1321 gaatcatggc agttacgctg actgaagccg ccgcgaagca cgtccagaag ttcctcaccc
     1381 ggcgcggaaa gggcgtcggc ctgcgcctcg gcgtcaagac ctcgggctgc tcgggcatgg
     1441 cctacaagct cgagttcgcc gatgaggtca actccgacga ccaggtctgg gaaagcttcg
     1501 gcgtcaaggt gatcgtcgac gagaagagcc ttccgtacct tgacggcacc gagcttgact
     1561 acacccgcga aggactccag gagggtttca agttccacaa cccgaatgaa aagagccgct
     1621 gcggctgcgg cgagtccttc cacgtttgat ggatcatcgg ggatccgcac gaaaggcggg
     1681 tccccttttg ggcaggaaaa gcaagaaaag gacaggaaga accgacgatg gcgcagatga
     1741 gtccattcca gctgttcggc atgaagcccg ccttcatgat cgacgaagct aagcttgagg
     1801 cggcctatca ggccgccatg atgaaggtgc atcccgaccg ctttgcggac aggcccgccg
     1861 ccgaaaggcg cgtggccgag cagtggagca cccgcatcaa cgaagcgttc aagctcctca
     1921 agagccccgt gaagcgcgcg gcctggctct gcgagaacgc aggccgtccg atcgaggctg
     1981 aaaccaacac ccgcatgccc gccgacttcc tcatgacgca gatcatgtgg cgcgaagccc
     2041 tcgagtcggg cgacgcaggc gcggcggacg ccgtccgcgc cgaggcaggc ggggtgcgtg
     2101 cggacgtcgt tcgcgggctt gccgaagcca ttgacgaaaa ggccgactgg gccgccgccg
     2161 ttgatctcac gcgccgcctg atgttcatcg accgcttcct tgaggaagcg gcgcgcgact
     2221 ccaaataaaa agagaaggtc ccctcatggc tttatttcag attgcagaac ccggcctttc
     2281 cgccgccccg cacgaacacc gccttgcggt cggcatcgac ctaggcacca cgaactcctt
     2341 ggtggcaagc gtcatcagcg gctccacgga ggtgatcctc gatgaagggg gcgctgccat
     2401 cctgccctcc gtcgttcact acgccgccga cggcacagtg acggtcggcg cggtcgcact
     2461 gcgcgacgcc agccgcgatc ccaagaacac catttcctcc gccaagcgcc tcatcggccg
     2521 cacgatgaag gatctggccg acgtccacaa tctgccgtac gacctcgtcg acgatccgtc
     2581 gatggtccgc atcgccacgt ccgcaggcgt caagtctccc gtcgaggtga gtgccgaaat
     2641 cctccgctct cttgcaaagc gcgccgagga ccgtctcggc ggcgatttga cgggcgccgt
     2701 gatcacggtc cccgcctact tcgacgatgc gcagcgccag gcgacgaagg acgccgcccg
     2761 tcttgcgaac ctcacggttc tgcgccttct caacgagccg acggcggctg cgctcgccta
     2821 cggcctcgac aatggtgccg agggtaccta catggtctac gatctcggcg gcggcacgtt
     2881 cgacgtctcg cttctcaagc tcacgcgcgg agtcttcgaa gttctcgcta cgggcggcaa
     2941 ttcggcgctg ggcggcgacg acttcgacca ccgcgtcctc tgtcacgtca cggagagctt
     3001 ccgcggcggc aatcccaagg cccgcttcac ggttgacggc aagcctgccg acacgcttcc
     3061 cggccacatc ctctccgccg ccgaaaagcg ccgtctgatg gcgctctcgc gcgccgcgaa
     3121 ggaggccctc acgaaggacg agtcgacctg catccgcttt gcgttcgaca acggcgacga
     3181 ggtcgagctt gatctcacgc gcgaggaatt cgacgcaatg acctgccacc tcgtcaggaa
     3241 gacgatcgac acggtcgagg ccgttctgcg cgacgcttcc gccggcgttt ccgacgtccg
     3301 cggcgtggtg ctcgtcggtg gcgcgacgcg catgccctgc gtccgcacgg ccgtcgagaa
     3361 gttcttcggc ttcgccccgc tcgacgacat cgatcctgac aaggtcgtgg cgatcggcgc
     3421 cgcgatgcaa gccaacaagc ttgcaggcaa cacctcgggc gacgacgact ggctgctgct
     3481 cgacgtcacg ccgctttcgc tcggtctcga gaccatgggc ggccttgttg aaaaggtcat
     3541 tccgcgcaac agtgcgattc ccgtggcgct tgcccaggac ttcacgacct tccgcgacgg
     3601 ccagacggcc atggcgatcc acgtcgttca gggcgagcgc gagctcgtcg acgcctgccg
     3661 ttcgctcgcc cgcttcgagc ttcgcggcat tccgccgatg gctgcgggcg ccgcccgcat
     3721 ccgcgtgacc ttccaggtcg acgccgacgg ccttctctcc gtctccgcac gcgaaatgac
     3781 gacgggcgtc gaggcttcga tccaggtcaa gccgagctac ggtctttccg acgacgacat
     3841 cgtccgcatg ctgcaggacg gcaacggcag cgccgaagag gacatgcgcg cccgcgagct
     3901 tcgcgagcag caggtcgagg cccgccggct tctcgagtcc acgcattccg cactcgcctc
     3961 cgacggcgac ctgcttgacg atgccgagcg cgccgaggtc gacgcgcttg tcgaacgcct
     4021 caccgcgctc gtcatctccg acgacgtcga ggcggtcaag gcgggcatcg aggcccttac
     4081 gaagggcacc gagactctgg ccgagcgccg catggaccgt tcgatccgcg aagccctctc
     4141 gggtatgagt gtcgacgacg agatgttcga cgagaagccc gaagacgaat ccaagaacca
     4201 ttgacgattc actagaacat tcactggaaa gggtgatgaa atgccgaaag tgacagtgct
     4261 cccccacgaa acgctttgcc cggaaggcct cgtctttgag gctgctacgg gtgagaacct
     4321 ggcccgcgcg ctccttgcgc acggcgtcaa gatcgagcat gcgtgcgaat tctcgtgcgc
     4381 ttgcgccacc tgccacgtga tcatccgcga aggcttcgac tccctcgagg agcccgacga
     4441 caacgagctt gatcatctcg acaccgcttg gggcgccagc gtgctctccc gtctctcctg
     4501 ccagacgaag gttggcgagg gcgacatcac ggtcgaaatc ccgaagtaca gccgcaacca
     4561 cgcacgcgag gagtaagcca tgtctctcaa gtggacggac gcccaggcga ttgccgagga
     4621 actctatgac gcggatcccg atcttgatcc gctcacgctc tcctttgtga agctccacga
     4681 catgatctgc gcgctgcctg acttcgacga cgatcccgaa gcctccaatg aaaagatcct
     4741 cgaggggatc ctcatgaagt ggctcgacga gcgcgattga gcgcaggcgg tgttctggca
     4801 aagcaaaacg gcggtctccc gatgggagga ccgccgtttt tgtccgtgcg gcaggcgcgg
     4861 cgtccgtctc agagggcgct gaccttgacg agcgagtccg caagcgcgcg ggcgtcttcg
     4921 gccgtgcggg tgagcggaac gttgggaagg aatccgagtc cttcaacctt cttgtggaag
     4981 gtttcgtgcc cctcgtccgt gcaggcggac tggttgatgc gcgcatcggc catgatgacg
     5041 ccgatgcctg cggcgcgaag ggcatcgccc gtgtaaggct cgtcccagag gacgagcagg
     5101 gggcggtcgg ccttgagcga ctcggccttc aggtcgtcag gggcgcctgc gaagctacag
     5161 gagaggcctg cggccctgag gccttcgccg aaggccgtcc agcgggcggc ctcgggcgtg
     5221 acgatgagaa tctgcatgag gaggcctctt tcagtgatga ccgccgccgc aggagtggtt
     5281 cggcgagaag cgcggcagtt cgcccgcgat gtgggcggcc acggcttttt taaccgtttc
     5341 gatgccggcg gcgtagtaga cgtcgacgcc ttcctgcagg aagcccatga gcgggcgcat
     5401 gcccatgccg ccagcaagca ggacggtgac gccgcggtcg gcgaggtaat tcacgggggc
     5461 gaggcagccg ccgtgctcgt gggcgcagga tgggatgact tcggacttca cgacggcacc
     5521 gtcctcaacg tcgacgagcg tgtagcagtc gcagtggccg aaatgggcgc cgacctgggc
     5581 ttcgaggccg ccgggaagaa cggacggaat ggcgattttc atgatgcttt ctccttgatg
     5641 ggaatgattc tgcttcggcg gcctccgcgc acgagcgcga gcaggcggtc ggcaaagttt
     5701 tggaagtggc cgtcgagctc cgacggcgtc tcgccgacgg cttcgccgcg gctcatggcc
     5761 tcggtgaaga cgggcgtgaa cggaagggtg ccgaggactt cggcgcctgc gtcgcgggcg
     5821 gcctgcatgg cggcctccgt cgcttcggcg ctgaggtcgg ccttgttgac gatgacggct
     5881 atcggcagcc tgaagcggcg tgcaagttcg gcaagtcgct tgaagtcggc gatgcccgag
     5941 gcgcccgcct cgatgaccgc gcagacgagg cctgcgcccg agagcgagct gatgacgggg
     6001 cagccgatgc cgggcgtgcc gtccgagagg atgatttcgg cgccgtccgc ctgggccgcc
     6061 ttggctgcct cctgcttgag gagcaggatg aggcggccgg agttttcggc cccgggcgtc
     6121 agttcggcgt ggatcatcgt gccgaagcgc gtgcggctgc ggtagtactc gccgcagtgt
     6181 ctgggcacga agtcgaaggc cagctcgggg cagagcttca cgcagacggc gcagccttcg
     6241 cagagctgct cgatgacagg gtattcgcaa ggagccgcct gcttgatcgc gtcgaacctg
     6301 cagaaggcaa ggcactgggc gcagtcgatg cagttgtcgg agttgaagac ggcgagatgc
     6361 ccgctcacga agtcggcgcg catctcggtc tcgggcttga gaagaatgtg aaggtcgggg
     6421 gcgtcgacgt catagtcgca gactacgatc ttctcgcggg cgctcatggc gcgcgcaagc
     6481 gctgcggtga cgctggtctt gcctgcgccg cccttgccgc tcagtacgac gatctcatgc
     6541 atgacggcct ccctcgaaaa gggtgctgag ggtgcgagcg gcttcggaca ggcactcgcg
     6601 ccaatcctcg gagtcgagca tcggatggcg tcctgcggca gcgcgtgcgg cggcctcgcg
     6661 atcaaagggc aggcgggccg cgacgggcag gcctttttcc ccgcagaagg cgatgagctc
     6721 cgcttcacaa gcggcggcgg aaggctggcc gcttcggttg atgacagcgg ccatgggctt
     6781 gcccagactt tcgaaggcgc gcagcgcgag cttgaagtcg tgtacgccga aaggcgtcgg
     6841 atcgatgacg agcacgacgg cgtccgcgga ggccgccgcc gtcatgacgg gacaggagac
     6901 gccgggcggg gcgtcgatga ggaggtcggc cttctcacgc gcggcgcagg ccgaggcctt
     6961 gctcagaagt gccctgatga ggggaggcgt catcgcctcg cccacgcgcg agcggccctc
     7021 gaggaagatg cgtccgtcct caagcgcgcc gaaaccgatg cggccgagct cgcgttcgcc
     7081 cgtcacgatg gcgtgcgggg cgcagacggc cgtgcagccg ccgcagccgt ggcacatatc
     7141 ggagaacggc atgatgcggc cgccgagctt cttgatggca ttgaactggc agatcgacgt
     7201 gcattcgccg cagaggtcgc aggcgtccgt aacacgcacg ggtacctcga gccccacggg
     7261 cgtttcctcg gcgaacgcgt gcgggaggta gagcccgagg tcgggcgcct cgacgtcggc
     7321 gtcggcgagg acggcgggat tcggccagaa ggcggcgatg cccgcagtga cggtggtctt
     7381 gcccgcaccg cccttgccgc tggcaaagag aattcgcatc aggcttcctt gttcgaggga
     7441 agaatgacgg tgagctcgcc cgcctcgaag gcgcggacga cttcgcccac cgtgcggttg
     7501 tcgacgtcct ggacggtgcc gatgccgatc gcctgcagcg catcgaaggc cttgggaccg
     7561 atgtagccgc tcagcgcgac cgtggcgcct gcgttcgaga cggtctcgac ggcgatgagg
     7621 cccgcgccct gtgcggcggt ctggcccgca ccattttcga tgtattcgac ggccttcgtc
     7681 tccgtgtcgt agatgatgaa gccggcggcg cggccgaagc gagggtcgac gtcgtcctca
     7741 agcgtggggc cttcggcgga aatggcgatc ttcatggcgg gatgctcctt gagcagctcg
     7801 ccggattcgc gacgacacga gttcgtgccg cccgtgacga cgagcggaat gttgttgacg
     7861 agactcaggg ccaccttctt cctcgcagag gcgaggacgc ggccgaaagt gtggcgcgag
     7921 acgcccatgc ccgagcttgc ctcggattgg gagagccctt caaggtcggc cagacgcagc
     7981 gcctcaagct cctcgaactt gaggctgacg cgctcggctg aggcgggaag cttccctgag
     8041 ggcttgaagt agacggtgcg catcggcatg tcgacgatgc ggctgcgcgg aggacggctc
     8101 aagttcaggt ctcgctagtt aatgctcaaa tgagcataac tcccaaaggg gttgaagtca
     8161 agcaaagacg actgcaatct tgactttata agtcgacata ttcaattcgg gcgcgaaagg
     8221 cggcgttcgg aaacgtcggc ggaggacgtg aaagtcagtc ggcgtgaagg aggtgctcga
     8281 ggatgagtcg ggcggcctcg ggcatgatgc gtccttcctc gatcatggag aggacttctt
     8341 cggtgttgaa gacggcgaag cccgcggcct cgccgtcgag gttgacgggg gcgtcgcagg
     8401 gggagagcct gaggatgaag cctgcggtgc tctcctgcat gcggccttcg ggaacgtcgt
     8461 gaaggacctg ccaggagagg tgtgcggggg agtgaaggcg ggagaattcg gggtcgagcc
     8521 ccgcctcctc atggagttcg cgcagcatgg cttcggcggg cgtttcgccc gaggagacga
     8581 ggcctgcggc gagattgtcc cagaggccgg gaccgatcga cttggagagg cttctctgct
     8641 gaaggagcag gcggccgtcg ggtgcgaggg cgagaagcct cacgacgacg gtggcgaggc
     8701 cgagtgcgcg gaagaccgag cgttcggcgc gggcgacgat gcggccgcgg gcatctctca
     8761 cgtcgaggag ctcgaagggg cgcttctggg tgaggccgtg gtgtgcaagt ccggccgtca
     8821 gggccgcaaa cgcgggctcg aggccttcgg gcgcgaaaag ctcgaagacg cgcgggcgca
     8881 cctcgggtgc gagggcccga agttcgggat ccgtgcgcag ggcgtgcatg acgtcttcgc
     8941 gcaggcttcc gagctcgagt cctcgagcga cgaaagcccc gagggactcg gggctttcgg
     9001 tctgcatccg ggtgcgaagc tccgcgagga gttccgcagc ccggatgcgt cggatcgagt
     9061 cgagtcgatc catgggcttc ttagttgccc gattccggac gcatgtgtgg gaagagcagc
     9121 acgtcgcgga tcgtcggggc gtcggtgagg agcatgatga agcggtcgat gccgacgccg
     9181 cagccggcgg tcgggggcag gccgtactcg agggcgcgga tgtagtcggc gtcgtagtac
     9241 atggcttcgt cgtcaccgtg gctcttcgca tcggcttggg ccttgaagcg ggcggcctga
     9301 tcgtccgggt cgttgagctc ggagaagccg ttgccgagtt ctcggccggc gatgaagagc
     9361 tcgaagcgtt ccgtcacgtc gtggttggcg tcggaggcgc gggcgagcgg ggagatctcc
     9421 gtcggatagt cgatgatgaa ggtcggctgg atgagcttgg cttcggtcgt ctcctcgaag
     9481 agggccatct gcagggcgcc gaggccgacg tagtcgggct gcggctcgcc gagacggacg
     9541 agttcggcgc gcaggaaggc ttcgtcggcg agctgttcgg ccgtgtagtc gggcgagtac
     9601 ttgaggatgg cttcaaccgg ggtgaggcgg tcgaagggct tctcgaggtc gatctcatgc
     9661 ccctggtagg gcaggatggc ggagccggtg gcttcgcggg cggccgtgcg gagaaggtct
     9721 tccgtgaact gcatctggtc gcggtacgtc cagtacgtgc agtagaactc catcatcgtg
     9781 aactcggggt tgtgacggac ggagacgcct tcgttgcgga agttgcggtt gatttcgaag
     9841 acgcgctcga agccgcccac gacgaggcgc ttcagataga gttccggcgc gatgcgcagg
     9901 tacatgtcca tgtcgagcgc attgtggtgc gtgatgaacg gcttggcgtt ggcgccgccc
     9961 ggaatcgggt ggagcatcgg cgtctcgacc tcgaggaagc ggtgcgcgag catgaagctg
    10021 cggatcgcgg cgatggcctt ggagcggacg aggaagcggt tgcgggagtc ctcgttcatg
    10081 atgaggtcga cgtagcgctg gcggtagcac atttcggtgt cgacgagacc cttgtgcttg
    10141 tcgggcagcg gacggatgtt cttcgtcatg aggcgaagct catgggcacg gatggagagt
    10201 tcgccggtct tcgtgcggaa gagcgtgccc ttgacggcga cgatgtcgcc gaggtcgaac
    10261 gtcttccagg cggcataggc ttcttcgccg atgtcgccct tcgtgatgaa gtactgcatg
    10321 tcgcccgtga agtcgcggac ggtcgcgaag ctcgccttgc ccatgatgcg cttgagcatc
    10381 atgcggccgg agcaggccac ttcgggcttg agctcctcga gctcttcggc ggtggcgtcc
    10441 tggtacttgg cgcggatgtc gccgaagagg tccgtgcgaa cgaagtcgtt cggataggcg
    10501 acgccggctt cgcgcagggc ggcgagtttg gcgcggcgct cggcgatgat gtggttttct
    10561 tcttccgcca actgctgggc ggtttcctgg ttctgagtct gttcggacat tttgtgttcc
    10621 tgtggtgagg ccgcggcgga cgtgccgtcg cggccttcct agaagcggat cgtgtctcag
    10681 gcggcggcga cgccgttctt cagagacgct tcgatgaact ggtcgagatc gccgtcaagc
    10741 acggcgcccg tgttgccggt ttcgacgttc gtgcggaggt ccttcacgcg ggactggtcg
    10801 agcacgtagc tgcggatctg gtgaccccag ccgatgtcgg acttgctctc ctcgagcttc
    10861 gtctgctctt ccatgcgctt gcgcatctca agctcgtaga gcttggcctt gagctgctgc
    10921 atggccttct cgcggttgcg gtgctgggag cggtcgtcct gacagatcgt gatgatgccc
    10981 gtcggaatat ggtggattcg gacggcggac tccgtcttct gaatgtgctg accgccggcg
    11041 cccgatgcac ggaagacgtc cacggagagg tcggcggggt tgatctcgat ttcgatcgag
    11101 tcgtcgactt cagggtagac gtacacggac gtgaaggacg tgtggcggcg ggcgttcgag
    11161 tcgaacgggc tcttgcgcac aaggcggtgg atgccggttt cggtgcgaag cgtgccgtag
    11221 gcccattcgc cctcgatgta gagcgtgcag gacttgatgc cggcgacttc gcccgggctt
    11281 tcttcttcaa gggtgacctt gaagcccttg cgttcggcgt agcgcatgta catgcgttcg
    11341 agcatgctcg cccagtcctg ggcctcggtg ccgcctgcgc ctgcctggat ttccaggtag
    11401 cagttggccg agtccatcgg ctggttgaac atgcgcttga attcgagctg agcaacctcc
    11461 ttctcgattt cctcgacgtc ctggcccacg gcttcaaccg tgtcccagtc ttcttcggcc
    11521 atcgagagtt cgaaaagctc ggcagcatcg ttgatgcccg aggtgagctt gttgaagctg
    11581 ccgacgatgt cgtcgaggag cttcttttcc ttgctcactt cctgagcatg tttgggatcg
    11641 ttccagagat ccgggttctc aacctcgcgg ttgacttctt cgagacggcg cgctttgcga
    11701 tcgacgtcaa agataccccc gaagttcggt cagacggcgc gtaaggtcgt ccgtaaggtt
    11761 ctggatctgg ttgatgcgtt cgacttccat catgtaactc tgctttttgt aggggtggaa
    11821 aaggaatggc cgcacccgat cagggcgttc gctggaggcg aagcgcggag tgcggctgaa
    11881 aaatcaatca attataaggc gaaagcgcct gcggtttttg ccgcaggcgc cctcaaaagt
    11941 gcacgaaagc cttggaaaat caaagctctg cggcctcgat gacgagctgc acgctccttc
    12001 tgcccatgaa ctcgttgatg ttcgggcgat aggcgaggcg caccatgccg gggagcgtct
    12061 ccgtgtggcg gaagaagatc gcgtcgaagc gctggccgcc gagctcgagg atgagcttgg
    12121 tgtgggcgtc cttcacgagg ctctggcgca cgacgcggaa ctcgttcgca aagacgggcg
    12181 cgtcgaagcc ctggccccag atctggctgt cgatcgcctg cacgaggcgc tcggtgatct
    12241 cgtcgggggc gaggccgccg tccgtgtaga tcacgcgctc gaagacggag gcgtcgacca
    12301 tgctcctgac ggccttctcg aatgcgtcgc ggaaggcggg caggtcgtcc tcgctcctga
    12361 gcgtgaggcc tgcggccatg gcgtggccgc cgaagcggag gacggcgccg ggcagggcct
    12421 tcgagacgag gtcgagcgcg tcgcgcaggt gtacgcccgc gatcgagcgg cccgaaccct
    12481 tcagcgcgcc gtcgtccgtg ggcgcgaaga cgatcgtcgg acggttgacc ttttccttga
    12541 ggcgggaggc gacgaggccc acgacgccct cgttgaagcg gccgtcgtag agcgtgagcg
    12601 tcgcaaggct gtcgacgttg ctgcacgaga gcgcggtctc ggccgcctgc tgcatctccg
    12661 tctcgagctc ctgacgggcg gcgttgaagt cgttgaggct gcgtgcatag tcgagcgcga
    12721 cgacgggatc atcggcaagg aggcactcga tgccgttttc catcgtgtcg agacgacccg
    12781 ccgcgttgat gcggggcccg agcgcaaagc cgaagtcgcg cacgcccgcg gcctcgggct
    12841 tcttgcccgc gacggcgaag agcgcgctga tgcctgcgtg cgtgcggccg agcctgatgc
    12901 ggttgaggcc ctgggcgacg aggatgcggt tgttcttgtc gagcttcacg acgtcggcga
    12961 cggtgccgag tgcgacgagg tcgacgaggg cgtcgagcct gggctgggtc tgctgcgtgt
    13021 agacgcctcg ctcgcgaagc agcgccctga gggcgaggag cacgtagtac atgacgccca
    13081 cgcccgcaag ggccttcgag gggaaggccg agtccgcgag gttcggattt acgatgcaga
    13141 cggcgggcgg aagctcggcg ccgggcagat ggtggtcggt gacgatgacg tcgatgccga
    13201 gctccgcggc atggcgcacg gcttcggcgg aggagacacc gttgtcgacg gtgaggagca
    13261 cgtcggggcg cggcgtgcgt gcggcgagca ggtcgaccac ggcgcaggag aggccgtagc
    13321 cgtgatggac gcggtgcggc acgaagtagt cggccttcac gcccatcatg ccgagtcctc
    13381 ggatcgccac ggcgcaggcg gtggcgccgt cgcagtcgta gtcggcgacg acggtcacgc
    13441 gttctccgcg ctcacgcgcg agcgcaaggc gttcggcggc ctcgcgggtg ccctcgagca
    13501 tggcgggcgg cagcatcgac ttccagtcct gggcgagctc ggaggcgtcc ctgatgccgc
    13561 gtgcggaaag cgcacgtgcg acgggcggca ggtagccctc ttcatggagg cgtcgcgcgg
    13621 cctcaagatc gaattcacgg ttgaggatgc gggtcattgc acggctcctt catagcggtc
    13681 ggggcaccag gcggcgggtt cgacggagga cttcctgcgg aagagcctct ccatgaggcc
    13741 ggcttcggcg ggggcgagcg tggcggcgcc cgtctcgccg aaggcgacga cgaggacgtc
    13801 gtcggcttcg aatctctttg cgtcctccct gtaggaggcg gccttgcggg cgacctcggg
    13861 caacgcgcgg gcccaggcct cccagtcgcg tgcgagccag gcggggtaga ggtcctcgat
    13921 cacggcgacg acgtcgccgt ggggcgcctc gggccagcgg cccgtgtccc tgccgatgcg
    13981 ttcgatgagg atgcctgcgg cattggccca gccgcggacg gcggcgtcgt cggaggcgac
    14041 gctgcggatg agcgtgggcg gaaagaagag ctcctcgtgt ccgccgcccg aaatccagag
    14101 ggcgtcgacg gcgtgcctgc ctgcggcgcg gcgggcggcg ttgacgggat ggtcggcgag
    14161 aat
//
LOCUS       DBMDTI010000026        13968 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1354_length_13968_cov_2.278719, whole genome shotgun sequence.
ACCESSION   DBMDTI010000026 DBMDTI010000000
VERSION     DBMDTI010000026.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 13968)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13968)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13968
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1354_length_13968_cov_2.278719"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="ACZPB6_02640"
     CDS             complement(<1..965)
                     /locus_tag="ACZPB6_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR1183411.1"
                     /translation="MNDPHGLALNRRDLLKSIGTLSVAGSTAGMFVPIAGAAEAAKEW
                     TGYSICDHCNHVPSCGIKFHARGNVIESIENWKENPRHILCSKGLATFQRLYNPNRLL
                     HPMKRTNPKGSEDPGFVRCSWEEAYRMIAENLLRIRKQYGADSVMFYAGDPKEPRPPI
                     IRLARHFGSVHWANESSAACRAGCMQAEQLTWGRPSTGAMPTEKTRSFMIMATNVWAQ
                     PLGWWESLVKAKARGCKIITVDTRRTKAAELADIHLAPRVGTDSALAMGMIRVLIKEG
                     LYNREFVEKWCHGFEELAAYCETFTPEKTEEITGVPAADIVAAAR"
     gene            1107..2876
                     /locus_tag="ACZPB6_02645"
     CDS             1107..2876
                     /locus_tag="ACZPB6_02645"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1183412.1"
                     /translation="MSRLTCTVLLAALALQCAPTLAGTPVRIAVTSGGHGTTSLKPLD
                     QSVRALEARFGPSNVTIRERVTLTELGHMLERGEVDYFISTSGVSRRNLSEGSRDLLT
                     ITDSRFPDPNKSYGSLFFTRRGSGIKNLEDMRGRTLVANHPNGPYSYLVGMGEIERAG
                     YDASTFFKSQRFLMQSAEDVVQAVLDGRADVGIVASCILEDFFPAVSEARRNLEPVAL
                     KAAAPCMRSTDQHPNWTLSAVRGADPETTREVVLTLLGMPASPDGVRWSITTDFQSTD
                     RVFKAFRTGVYDVMNERSFSDFVEKYWLFAAVPLAAALLCFGFAALLRRMVGIRTREL
                     KRTLENERRLRREAQEADERYRRLLRVGLLGQLSTMFAHEIRQPVGAILAYVQGLRNF
                     CASGSLTPEMSEGVLAKINAQAERVSDIVERVRLYARTDAVDRITTDLRDVVDEAVGP
                     FKRNTRFEGKIILEPPASSEKFTVRVSPLEISIALHNLMQNAVDALASAGTADPRITI
                     RIERTEERLAVVVSDNGPQLTQDEVARMTAPLHTTKSSGMGLGLILVNSIATGHGGHL
                     RLSPAACGGLEARLILPHAGEHD"
     gene            2869..3477
                     /locus_tag="ACZPB6_02650"
     CDS             2869..3477
                     /locus_tag="ACZPB6_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1183413.1"
                     /translation="MIEKTQMLIRIVDDDEPFADAICFLLLSKGLRAVVHASAEKFLE
                     EDDPSVPGCLILDYQMPELNGIETQRKLLDKGWRWPVVFMTAHADVDMAVTAFKQGAF
                     DLLRKPVAPDVLIAAVESSLEKVRAGMQHHHDEVCGLFEGLTNRERQIVKLAAAGLAN
                     QQIADRLGISERTVEAHRSAAYRCLNVKCLEELQAFIRDMDD"
     gene            complement(3488..4090)
                     /locus_tag="ACZPB6_02655"
     CDS             complement(3488..4090)
                     /locus_tag="ACZPB6_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014651.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorD/DmsD family molecular chaperone"
                     /protein_id="HIR1183414.1"
                     /translation="MSDEFNLVNDLAESRAQVYGAYADFFSGNGSSGFAGNWSELVRY
                     QTGVEALLEMTSGPAPEPLPEPIVMDRLYARLFQGVGERTVPLCASAYTGEHRMMCGA
                     DCRRMLEAYRAFGFLPSEADAPFADSLGVELAFMSLLAQMKAPAGKQREFLTACLIPL
                     ASTVAHELQGLETGAVGDVLAMLLRTMKSDGMLLERADAC"
     gene            complement(4099..5370)
                     /locus_tag="ACZPB6_02660"
     CDS             complement(4099..5370)
                     /locus_tag="ACZPB6_02660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1183415.1"
                     /translation="MNSLQLRTAFRRLFAQWYLLGLPARCLQFTLIFEAVSHGVGAAW
                     MGAVMAASSVACAVFTIYAGRLYNRFGPSPLNRCAALCCAGAGFLATQHEAPAALAVA
                     WMLTGMTLMLAATAGYRGIGGLFGAADRVSRFSRMCLLSNVSEIAMPVAIGALFSQAP
                     DLMPFIVLAVSLALSFLSSGVSRSDLRAHGLVESTDLLVNIRRLAGSGPLLAGVVAGS
                     GVNALFCILDILVPTAGGGMGLTTIQTGTLLSACALAQVAASSLLSVRANHRTLLPQL
                     RNALLLGGVALSGAALADGFASFLLVVSVAGFGFGLVKPLSMSTLFQYAPPESVGDIV
                     GMRFMLNNLGRIMMSLFMAAGVGGFMSSTVFLSTLALIVLVVTIGASIRRGPQERLRG
                     DVRGKQVGVSLMRRTVPTYPGAYNSRGMLDT"
     gene            complement(5577..5762)
                     /locus_tag="ACZPB6_02665"
     CDS             complement(5577..5762)
                     /locus_tag="ACZPB6_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183416.1"
                     /translation="MSRNVNIYKSSNGRQYVRVSESYRNADDDMLAAFGLKPLCRLEE
                     KASLRRKLRLKTFPPMA"
     gene            complement(5936..7414)
                     /locus_tag="ACZPB6_02670"
     CDS             complement(5936..7414)
                     /locus_tag="ACZPB6_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR1183417.1"
                     /translation="MSENQRIEFGHLPVEERIKSFRDFTIPLNDDEVHAQSLRCLHCG
                     TPFCSNNCPLHNRPVDWNRLVREGDWERAWACLSATNSFPEFTSRVCPAICEAGCTRY
                     LLDDAAVGIRSIERAVIDRAWREGWVRPEPAAVRTGRRVAVVGSGPAGLACAQQLARA
                     GHDVTVYEKSERPGGLLRFGIPGFKLEKDVVERRISQLEAEGVRFELSVVVGDRKFEA
                     GVHNAAKRSVKAADLLGEYDAVVLACGSEVPRDLKVPGREGRGVFFALDVLQEQNRVD
                     EGLVKKPAFDCRGCDVVIVGGGDTGSDCVGTARRQGAGRIWQLDLSPMPPEKDDKSCW
                     PAWPRKLRTFTSHEEGCERLWEASTKEILLNDKGEVRGVRCVKLKWSLGPDGRFTPSE
                     VPDSAFEIPCQRVLLAMGFMHPAAATLKSFGVETDARGNAKAGVDGSEAPFATSVPGV
                     FACGDTRSGQSLVVRAMSEGRRCARAVDVFLTGDSELPSFRP"
     gene            complement(7426..12096)
                     /locus_tag="ACZPB6_02675"
     CDS             complement(7426..12096)
                     /locus_tag="ACZPB6_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR1183418.1"
                     /translation="MTRQKEEARREAGLKGLYRSELEHDACGVGAVVNIKGRASHKVI
                     EDALLILHNLDHRGAVGADPLCGDGAGILIQIPDAFYREEMRAQGVELPAVGDYGVGM
                     IFLPQEAASRAACEQELERSVRREGQVVLGWRDVPVCEVMTMSPTVKASAPVIRQIFI
                     GRGPDVMVQTALERKLYVIRKTASHRIQALKLTHGSEYFVPSMSTRTVVYKGLLLADQ
                     VGQYYLDLQNEKVESAIAVVHQRFSTNTFPSWALAHPYRYIAHNGEINTVRGNNFWLR
                     AREQVWQSPVLGGEIRKLAPIIYDGQSDTASLDNAIELMVMSGYSLAQAMLMLVPEAW
                     EKSTTMDPDRRAFYAYHAPMMEAWDGPAAIVFTDGVQIGATLDRNGLRPARYVLTDDD
                     TVVLASEVGVLPIPESRIRRKWRVEPGKMLLIDTGRGRIIEDDEIKNQLALAKPYREW
                     VDRLNLRLADLAGAAEAEPDEAVGPERVRELQRVFGLTQEDVDLILVPMARKGMEPVG
                     SMGNDVSLPVLSKKSKPLSQYFKQLFAQVTNPPIDPIREELVTSLISYIGPKPNVLDV
                     NDVEQTRRIEVSQPVLTDAEMRKLLSISDYTSGKVRSKVIDITYPAAWGSAGVEACLA
                     GLCAQAVDAIKLGHTILILSDEAVSKDRVAIPALLATSALHQHLIAEGLRTTTGLVVS
                     SGSATETHDFALLMSYGAEAVHPYMALMTLKDLAPKAGLSPDEAVAHYVKAVGKGLTK
                     VMAKMGISTLMSYRGARIFEAIGLQSAFVERYFTGTPSQIDGIGLFEVMQEAINLHKA
                     AYGIGAAPGRDLPTGGEYAWRADGEEHMWTPEAIVKLQKASREKNWNSYREYADIIND
                     QSRRQMTLRGLLRFRTEGCTPVPLDEVEPAKDIVRRFATGAMSLGSISAEAHATLAVA
                     MNRIGAKSTSGEGGEDPRRYEAEMMTGLSPVKAGQTLADVLGSDRIVADVKLEAGDSL
                     RSKVKQVASARFGVTAQYLASADQIQIKMAQGAKPGEGGQLPGHKVTEYIAELRYSVP
                     GVGLISPPPHHDIYSIEDLAQLIHDLKTANSAADVSVKLVSETGVGTVAAGVAKAKAD
                     HIVISGHDGGTGASPLSSVKHAGSAWELGLSEAQQTLVVNGLRNRVAIQVDGQIKTGR
                     DVVIGAMLGADEFGFATAPLVVEGCMMMRQCHLNTCPVGIATQDPELRKRFRGKPGAV
                     INYFFFVAEEARRIMAELGIRRFEDLIGRSDLLVQRDDIDNWKAKGVDLSRILHRPSA
                     EPCAIRHTLAQDHQLEKNLDHALLPRFACAIEGGETVSITTAVKNTHRSVGTMISHEI
                     VKRHGAAGLPDDSIRIALHGTAGQSFGAFAAKGLTLDLSGDANDYVGKGLSGGRIIVR
                     APADFHGRPSENIIAGNATLYGATSGEAYFAGVVGERFAVRLSGASAVIEGCGDHGCE
                     YMTGGTTVVLGRTGRNFASGMSGGVAFVFDADGSFEANCNPSMVALERVMSHEEQVRT
                     SDPRTWHMGCTDEELLRRLITRHQLFTDSTLAAELLEHWSSTRARFVKVIPLQYRAVL
                     QSRA"
     gene            complement(12718..>13968)
                     /locus_tag="ACZPB6_02680"
     CDS             complement(12718..>13968)
                     /locus_tag="ACZPB6_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183419.1"
                     /translation="AERLQADGANLGLSEEQAERLRSAAEGAARFSYDRPATVDAIML
                     MTERLRNRFGLNRKAGAGQLDVRADAQRVVWMLDHVFFSATVLRSDIEAAQYFQGENN
                     RGASMQLVDLVKSLNLRFVPEAELEKYAALGTNLAKERPWGTFQGSDAVVRVLLPSML
                     IVRGLWTFWAHWNDPADYRHFMSGGGALARKRMSDSRGGTQGEATFPTLSSMPRAGVP
                     FFRTLVWSAVLLAAVRQTLDTEADDYKKYITGQELEPLLCNGLVYWADAFMTDGDGSM
                     MKRFDDFMALALEDPSQVNPDRLAEADIVKECADRLSRDSAYEGWREALLLVMEMVRL
                     SWGRLDWTRVVKALALERTGECLLLAPYAGGRAEETAEELLRRARLRPWDLAGAGWES
                     RMAVFEAYDACGSRSESIAVVFKS"
BASE COUNT         2523 a         4561 c         4430 g         2454 t
ORIGIN      
        1 atgcgggccg cggccacgat gtcggcggcg ggcacgccgg tgatctcctc ggtcttttcc
       61 ggggtgaagg tctcgcagta ggcggcaagc tcttcaaagc cgtggcacca cttctcgacg
      121 aattcgcggt tgtagaggcc ttccttgatg agaacgcgga tcatgcccat ggcaagcgcg
      181 ctgtcggtgc ccacgcgggg cgccagatgg atgtcggcca gctcggcggc cttcgtgcgg
      241 cgggtgtcga ccgttatgat cttgcagccg cgggccttgg ccttgacgag ggactcccac
      301 cagcccagcg gctgggccca gacgttcgtc gccatgatca tgaaggaacg cgtcttttcg
      361 gtcggcatgg cgcccgtcga cggacggccc caagtgagct gctcggcctg catgcagccg
      421 gcgcggcagg cggccgacga ttcgttggcc cagtgcacgg agccgaaatg acgggcaaga
      481 cggatgatgg gcggacgcgg ctccttcggg tcgcccgcat agaacatcac gctgtcagca
      541 ccgtactgct tcctgatgcg cagaagattt tccgcaatca tcctgtaggc ttcctcccag
      601 gagcagcgga cgaatccggg atcctcgctg cccttgggat tcgtgcgctt catcggatgg
      661 agcagacgat tcgggttgta gagtcgctgg aacgtcgcca gccccttcga gcagaggatg
      721 tggcgggggt tctccttcca gttttcaatc gattcgatca cgttcccgcg tgcatggaac
      781 ttgatgccgc acgacggcac gtggttgcag tggtcgcaga tcgaatagcc ggtccactcc
      841 ttggcggctt cggctgctcc ggcaatcgga acgaacatgc ctgcggtcga gcctgcaacg
      901 gaaagcgtgc cgatcgactt gagcagatcg cgtcggttga gagcgagtcc atgggggtca
      961 ttcatggctt tactcctttt cggtcaatga aattcaaagg tctcctgtac acctccgacc
     1021 gtcctggtcc gggactgcga agatgacttc ctcaattttc atgttataac ttccgatacc
     1081 taagtacagc acctcccatc gcaaacatga gccgcctcac ctgcaccgtc ctgctggccg
     1141 ctctcgccct gcagtgcgcc ccgactctgg ccgggactcc cgtgcgcatc gccgtcacgt
     1201 cgggcgggca cggcaccacc tcgctcaagc cgctcgacca gtccgtcagg gcgctcgagg
     1261 cgaggttcgg cccctcgaac gtcacgatcc gcgagcgcgt cacgctcacg gagctcggtc
     1321 acatgctcga gcgcggcgag gtcgactact tcatttccac gtcgggcgtc tcgcgccgca
     1381 atctgtccga aggctcgcgc gacctcctca ccatcaccga cagccgcttt cccgacccga
     1441 acaagtccta cggctcgctc ttcttcacac gcaggggcag cggaatcaaa aaccttgagg
     1501 acatgcgcgg ccgcaccctg gtcgccaacc atccgaacgg cccgtacagc tacctcgtcg
     1561 gcatgggcga aatcgagcgc gccggctacg acgcgtccac tttcttcaag tcgcagcgct
     1621 ttctgatgca gtccgccgag gacgtcgttc aggccgtgct cgacggacgc gccgacgtcg
     1681 gcatcgtcgc gagctgcatt ctcgaggact tcttccctgc ggtatccgaa gcccgccgca
     1741 atctcgagcc cgtggccctc aaggcggccg ccccctgcat gcgctcgacg gaccagcatc
     1801 ccaactggac gctttcggcc gtgcgcggcg cggatcccga aacgacgcgc gaagtcgtgc
     1861 tcacgcttct cggcatgccc gcatcacccg acggcgtgcg ctggagcatc accaccgact
     1921 tccagagcac cgaccgagtc ttcaaggcct tccgcacggg cgtctacgac gtcatgaacg
     1981 aaagaagctt cagcgacttc gtcgagaagt actggctctt cgccgccgtg cccctcgccg
     2041 cagccctcct ctgcttcggc tttgcggccc ttctgcgccg catggtcggg atcaggaccc
     2101 gcgagctcaa gcggacgctg gaaaacgagc ggcgcctgcg ccgcgaggct caggaggcgg
     2161 acgaacgcta caggcgactt ctgcgcgtcg ggcttctcgg acagctgagc acgatgttcg
     2221 cgcacgaaat ccgccagccc gtcggcgcca tcctcgccta cgttcaggga ctgcgcaact
     2281 tctgcgcctc gggctcgctc acgcccgaaa tgtccgaggg cgtcctcgcg aagatcaacg
     2341 cgcaggccga aagagtgtcg gacattgtcg agcgcgtgag gctctatgca aggaccgatg
     2401 ccgtcgaccg catcacgacc gatctgcgag acgtggtcga cgaggcggtc gggcccttca
     2461 agcgcaacac gcgctttgag ggcaaaatca ttctggagcc gcccgcatcg tccgaaaagt
     2521 tcaccgtgcg cgtctcgccc cttgaaatca gcatcgcgct gcacaacctc atgcagaatg
     2581 ccgtcgacgc cctcgcctct gcaggcaccg cagatccgag gatcacgatc cgcatcgagc
     2641 gcacggagga gaggcttgcg gtcgtcgtct ccgacaacgg cccgcagctc acgcaggatg
     2701 aagtcgcccg catgacggcg cccctgcaca cgaccaagtc ctcgggcatg ggactcggcc
     2761 tgattcttgt aaactccatc gcaaccggcc atggcggcca tctcaggctg tctcccgccg
     2821 cttgcggcgg ccttgaggca cgactgatcc ttcctcacgc aggcgaacat gattgaaaaa
     2881 acgcagatgc tcatccgaat cgtcgacgat gacgaacctt ttgcagacgc gatctgcttt
     2941 cttttgctga gcaagggact cagagccgtc gttcatgcgt cggccgagaa gttcctcgaa
     3001 gaggacgatc cgtccgttcc gggctgcctc atcctggact atcagatgcc cgaactcaac
     3061 ggcatcgaaa cccagcgaaa gctgctcgac aagggctggc gctggcccgt cgtcttcatg
     3121 acggcccatg ccgacgtcga catggcagtc actgctttca agcagggcgc cttcgacctt
     3181 cttcgaaagc ccgttgcgcc cgacgtcctc atcgccgcag tcgagagcag cctcgagaag
     3241 gtccgcgcag gcatgcaaca ccaccacgac gaagtctgcg gcctcttcga aggactcacc
     3301 aatcgagaac gccagatcgt gaagctcgcc gccgctggtc tcgccaacca gcagatcgcc
     3361 gaccgcctgg gcatcagcga gcgcaccgtg gaagcccatc gctccgcagc ctaccgctgc
     3421 ctgaacgtca aatgcctgga ggaactccag gcattcatca gggatatgga cgactgaacg
     3481 tccgaaatca gcaggcgtcc gcccgctcga gcagcattcc gtccgacttc atcgtgcgaa
     3541 gaagcatcgc cagaacgtct ccgacggccc ccgtctccag cccctgcagt tcatgcgcga
     3601 cggtcgaggc aagcggaatc aagcatgcgg tcagaaactc ccgctgcttc cctgcgggcg
     3661 ccttcatctg cgcgagaagc gacatgaacg caagctccac gcccaacgag tccgcaaagg
     3721 gcgcgtccgc ctcggaagga agaaagccga aggccctgta ggcttcaagc atgcgacggc
     3781 aatcggcccc gcacatcatg cggtgctcgc ccgtgtaggc cgaggcgcaa agcggcacgg
     3841 ttctttcgcc aacgccctga aaaagcctcg catacagcct gtccatgacg atgggctcgg
     3901 gcagcggctc gggcgcgggc ccggacgtca tttcaagcag ggcctcgaca cccgtctgat
     3961 agcggaccaa ctcggaccag ttgccggcaa agcccgaaga gccgttgccg gagaaaaagt
     4021 cggcatatgc gccgtagacc tgcgcacggg attcggcgag atcgttcaca agattgaatt
     4081 cgtcggacat ggggctcctc aagtgtcaag cattcctctt gaattgtagg ctccgggata
     4141 cgtaggtaca gttcggcgca tcagcgacac ccccacctgc tttccgcgca cgtcacctct
     4201 gagtctctcc tgaggtcctc tcctgatcga agcgccgatc gtcacgacga gcacgatcag
     4261 ggcgagcgtg ctgagaaaga ccgtcgagga catgaagccg ccgacgcccg ccgccatgaa
     4321 gagcgacatc atgattcggc cgaggttgtt gagcatgaac ctcatgccca cgatgtcgcc
     4381 caccgattcg ggcggcgcgt actggaagag cgtgctcatc gatagcggct tgaccagacc
     4441 gaatccgaag cccgccaccg acacgacgag cagaaaggac gcgaatccgt cggccagtgc
     4501 ggcgcccgag agcgcgacgc cgcccagaag cagcgcgttg cgaagctgag gcagaagcgt
     4561 cctgtgattg gcgcgcacgg acagaagcga gcttgcggcc acctgcgcga gcgcgcacgc
     4621 cgaaaggagc gtccccgtct ggatcgtcgt gaggcccata ccgccgcccg ccgtgggaac
     4681 gagaatgtcg agaatgcaga agagcgcgtt gacgcccgag cccgccacca cgcccgccag
     4741 cagcggcccc gagcccgcga gtcgcctgat gttcacaagc aggtccgtcg actcaacaag
     4801 gccgtgcgcc ctgaggtccg aacgcgaaac gccggaggaa aggaaggaaa gcgccagaga
     4861 gaccgccagc acgatgaagg gcatgaggtc cggagcctgc gagaagagcg caccgatcgc
     4921 gacgggcatg gcaatctcgc tcacgttgct caacaggcac attctcgaaa agcgcgagac
     4981 gcggtccgcc gcgccgaaga gtccgccgat gccgcgatag cctgccgttg cggcgagcat
     5041 gagcgtcatg cccgtgagca tccaggcgac ggcaagcgca gcaggcgcct catgctgcgt
     5101 cgccagaaat cccgcgcccg cacagcagag cgctgcgcag cggttgagcg gtgaaggccc
     5161 gaagcggttg tagagccgcc ccgcatagat cgtgaagacg gcgcaggcca cggaactcgc
     5221 cgccatgacg gcacccatcc aggccgcacc cacgccgtgc gagaccgctt cgaagatgag
     5281 cgtgaactgc aggcaccgcg ccggaaggcc gagcaggtac cactgcgcga aaaggcgccg
     5341 aaaggctgtg cgaagctgca gcgaattcat ggagcgtccg attgagcgtg ccgaaggaag
     5401 gcacgaggcc tgctgcggac atttgaacca cctgtcgcgg aagcgttccg ggacagaaaa
     5461 attgttaatt ataaccccgt cgtacgctcc gaatggggca atattcgtga aagaccctat
     5521 gcgagagcgg aaaacgatgc acgatgctca ggcctgaccc gaagtgcggc cttccgtcac
     5581 gccatcggcg ggaaggtctt cagcctcagc tttctgcgaa gagacgcctt ttcctcgagc
     5641 ctgcatagag gcttcagacc gaaggcggcg agcatgtcgt cgtcggcatt tcgatagctt
     5701 tcgctcacgc ggacatactg ccgtccgttc gagctcttgt agatgttgac gttgcgggac
     5761 atggttgacg tgaatgcaaa gactttcgac agcttattgt acgtgtcgca gcagccgttt
     5821 ctcagaagtc gatcaagctc ggcgtccctg cgaacacatc cttcaacccc atgccaaacg
     5881 ggggcgcctc catcgacagg aagacgcccc cgcaggtccc ttcaacccga atgccttacg
     5941 gccggaagga cggcagctcc gagtcgcccg tgaggaacac gtccacggcg cgggcgcaac
     6001 gcctgccctc gctcatcgcg cgcacgacga gcgactggcc gctgcgcgtg tcgccgcagg
     6061 caaagacgcc gggcaccgag gtcgcaaagg gtgcttcaga gccatcgacg cccgccttcg
     6121 cattgccgcg ggcatcggtc tcgacgccga aggacttgag cgtcgcagcg gcgggatgca
     6181 tgaagcccat ggcgagcagc acgcgctgac agggaatctc gaaggcggaa tcgggcacct
     6241 cggacggcgt gaagcggccg tcgggaccga ggctccactt gagcttcacg cagcgcacgc
     6301 ccctcacttc gcccttgtcg ttcaagagga tctccttcgt ggaggcctcc cagagacgct
     6361 cgcagccttc ctcgtgggag gtgaaggtgc gaagcttcct cggccaggcg ggccagcagg
     6421 acttgtcgtc cttctcgggc ggcatggggc tcaggtcgag ctgccagatc ctgcccgcgc
     6481 cctgacggcg ggcggtgccg acgcagtcgc tgcccgtgtc gccgccgccc acgatgacga
     6541 cgtcgcagcc cctgcagtcg aaggcgggct tcttgacgag gccctcgtcg acgcggttct
     6601 gctcctgcag gacgtcaagg gcgaagaaga cgcccctgcc ctcgcgtccc ggaaccttga
     6661 ggtctctcgg cacctcggag ccgcaggcga gcacgacggc gtcgtactcc ccgaggaggt
     6721 ccgcggcctt cacgctccgc ttggcggcgt tgtgcacgcc cgcctcgaac ttcctgtcgc
     6781 ccacgaccac ggagagctcg aacctgacgc cttcggcctc aagctggctg atgcggcgct
     6841 cgacgacgtc cttctcgagc ttgaagcccg gaatgccgaa gcgaagaaga ccgccggggc
     6901 gctcggactt ctcgtagacc gtgacgtcgt ggcccgcacg ggcaagctgc tgggcgcagg
     6961 caaggcccgc agggcccgac cccacgacgg ccacacgcct tccggtcctc acggccgcag
     7021 gctcgggcct cacccagccc tcgcgccagg cgcggtcgat gacggcgcgc tcgatcgagc
     7081 ggatgccgac ggccgcgtcg tcgagaaggt acctcgtgca gcccgcctcg cagatcgcgg
     7141 gacagacccg gctcgtgaac tcgggaaagg agttcgtcgc cgagaggcac gcccaggcgc
     7201 gctcccagtc gccttcgcgc acgaggcggt tccagtcgac gggacggttg tgaagcggac
     7261 agttgttcga gcagaacggg gtgccgcagt gcaggcagcg caggctctgc gcatgaacct
     7321 cgtcgtcgtt gagcgggatc gtgaagtccc tgaagctctt gatgcgttcc tcgaccggga
     7381 gatggccgaa ttcgatgcgt tggttttcag acatggcaat ctcctttaag ccctgctctg
     7441 cagcacggcg cggtactgaa gcggaatgac cttgacgaat cgcgcgcggg tgctcgacca
     7501 atgttcgagc agctcggccg cgagcgtcga gtccgtgaag agctggtggc gggtgatgag
     7561 gcgcctgagg agctcctcgt ccgtgcagcc catgtgccag gtgcgcggat cgctcgtcct
     7621 cacctgctcc tcgtgactca tgacccgttc aagcgcgacc atggacggat tgcagttcgc
     7681 ctcgaagctg ccgtcggcgt cgaagacgaa cgcgacgccg cccgacatgc cggaggcgaa
     7741 gttgcggccc gtgcgtccga gcacgaccgt cgtgccgccc gtcatgtact cgcagccgtg
     7801 gtcgccgcag ccctcgatca cggcgctcgc gcccgaaagc ctcacggcga atcgttcgcc
     7861 gacgacgccc gcgaagtacg cctcgccgct cgtggcgccg tagagcgtcg cattgcccgc
     7921 gatgatgttt tcgctcggcc tgccgtggaa gtccgcaggc gccctcacga tgatgcgccc
     7981 gcccgagagg cccttgccca cgtagtcgtt cgcgtcgccc gacaggtcga gcgtgaggcc
     8041 cttcgccgca aaggccccga aggactggcc cgccgtgccg tgaagcgcga ttctgatcga
     8101 gtcgtcgggc aggcccgctg caccgtgcct cttcacgatc tcgtgcgaga tcatcgtgcc
     8161 gaccgaacgg tgagtgttct tcaccgcagt cgtgatcgag acggtctcgc cgccctcgat
     8221 cgcgcaggcg aagcggggca ggagcgcatg gtcgagattc ttctcaagct gatggtcctg
     8281 agcgagcgtg tggcggatgg cgcaaggctc ggcgctcgga cggtgaagga tgcgggagag
     8341 atccacgccc ttcgccttcc agttgtcgat gtcgtcgcgc tgcacgagca ggtccgacct
     8401 gccgatcagg tcctcgaagc ggcggatgcc gagttcggcc atgatgcgac gggcctcttc
     8461 ggccacgaag aagaagtagt tgatgacggc gccgggcttg ccgcggaagc gcttcctgag
     8521 ttccggatcc tgcgtggcga tgccgacggg acatgtgttg aggtggcact ggcgcatcat
     8581 catgcagcct tcgacgacga gcggcgctgt cgcaaagccg aattcgtccg cgccgagcat
     8641 ggcgccgatg acgacgtcgc gccccgtctt gatctggccg tcgacctgga tcgcaacgcg
     8701 gttgcgcagg ccgttgacga cgagcgtctg ctgagcttcg gagagaccga gctcccaggc
     8761 gctgcccgcg tgcttcaccg acgagagcgg gcttgcgccc gtgccgccgt cgtgccccga
     8821 aatgacgatg tggtcggcct tcgccttggc gacgcccgcg gccaccgtgc ccacgcccgt
     8881 ttcggagacg agcttcaccg agacgtcggc cgcgctgttg gcggtcttga ggtcgtggat
     8941 gagctgcgcg aggtcctcga tcgagtagat gtcgtgatgc ggaggcggcg agatgaggcc
     9001 gacgccgggg acggagtacc tgagttcggc gatgtattcg gtcaccttgt ggccgggaag
     9061 ctggccgcct tcgcccggct tggcgccctg ggccatcttg atctggatct ggtccgcgct
     9121 cgcgaggtac tgggccgtca cgccgaagcg cgccgaggcc acctgcttga ccttcgagcg
     9181 caggctgtcg cccgcctcga gcttcacgtc cgcgacaatc ctgtcgctgc cgagcacgtc
     9241 ggcgagcgtc tggccggcct tgacgggaga gaggccggtc atcatctcgg cctcatagcg
     9301 cctcggatcc tcgccgccct cgcctgaagt cgacttcgcg ccgatgcggt tcatggcgac
     9361 ggcgagcgtg gcgtgggcct cggccgaaat ggagccgaga ctcatggcgc cggttgcaaa
     9421 gcgtctgacg atgtccttcg caggctcgac ctcgtcgagc ggcacgggcg tgcagccctc
     9481 ggtcctgaac ctcagcaggc cgcgcagcgt catctgacgg cggctctggt cgttgatgat
     9541 gtccgcatat tccctgtacg aattccagtt cttctcgcgg gaggccttct ggagcttgac
     9601 gatcgcctcg ggcgtccaca tgtgctcctc tccgtccgcg cgccaggcgt attcgccacc
     9661 cgtgggcagg tcgcggccgg gggcggcgcc gatgccgtag gcggccttgt ggaggttgat
     9721 cgcctcctgc atcacctcga agaggccgat gccgtcgatc tgcgagggcg tgcccgtgaa
     9781 gtagcgctcg acgaaggccg actgcaggcc gatcgcctcg aagatcctcg cgccgcggta
     9841 gctcatcagg gtcgagatgc ccatcttggc catcaccttg gtgaggccct tgcccacggc
     9901 cttgacgtag tgggcgacgg cctcgtcggg cgagagcccg gccttgggcg cgagatcctt
     9961 gagcgtcatg agggccatgt agggatgcac cgcctcggcg ccgtagctca tgaggagcgc
    10021 gaagtcgtgc gtttcagtcg cggagcccga gctcaccacg aggcccgtcg tcgtgcgcag
    10081 gccctcggcg atcaggtgct gatgaagggc cgaggtcgcc aggagcgcgg gaatcgccac
    10141 gcggtccttc gagaccgcct cgtcggagag gatgaggatc gtgtgaccga gcttgatcgc
    10201 gtcgacggcc tgagcgcaca ggcccgcgag gcaggcctcg acgcccgcgc tgccccaggc
    10261 ggcgggatag gtgatatcga tcaccttgct cctcaccttg cccgaggtgt agtcggaaat
    10321 cgagaggagc ttgcgcatct ccgcgtccgt aaggacgggc tgcgagacct cgatgcgcct
    10381 cgtctgctcc acgtcgttga cgtcgagcac gttgggcttc ggaccgatgt agctgatgag
    10441 gctcgtgacg agctcctcgc ggatcggatc gatcggcggg ttcgtcacct gagcaaagag
    10501 ctgcttgaag tattgggaga gcggcttgga cttcttcgag aggaccggga gcgagacgtc
    10561 gttgcccatg ctgcccacgg gctccattcc ctttctggcc atcggcacga gaatgaggtc
    10621 gacgtcctcc tgggtgaggc cgaagacgcg ttgcagctcc cggacgcgct cggggccgac
    10681 ggcttcgtcg ggctcggcct cggcggcgcc cgcgaggtcg gcgagcctca ggttgaggcg
    10741 gtcgacccac tcgcggtagg gcttggcgag ggcgagttgg ttcttgatct cgtcgtcctc
    10801 gatgatgcgc ccgcggcccg tgtcgatgag gagcatcttg cccggctcga cgcgccactt
    10861 gcggcggatg cggctttcgg gaatgggcag gacgcccact tcgctcgcga gcacgaccgt
    10921 gtcgtcatcg gtgagcacgt aacgcgcggg cctgaggccg ttgcggtcaa gcgtcgcgcc
    10981 gatctgcacg ccgtccgtga agacgatggc ggccggaccg tcccaggcct ccatcatcgg
    11041 ggcatgatat gcgtagaagg cgcgcctgtc gggatccatc gtcgtcgact tctcccaggc
    11101 ttcgggcacg agcatgagca tcgcctgcgc gagcgaatag cccgacatca ccatcagctc
    11161 gatggcgttg tcaaggctcg ccgtgtcgga ctggccgtcg tagatgatcg gggcgagctt
    11221 gcggatctcg ccgccgagca cgggcgactg ccagacctgc tcgcgggcgc gcagccagaa
    11281 gttgttgccg cggaccgtgt tgatttcgcc gttgtgcgcg atgtagcggt acggatgcgc
    11341 aagagcccag ctcgggaagg tgttggtcga gaatcgctgg tgcacgacgg cgatggcgct
    11401 ctcgaccttt tcgttctgca ggtcaaggta gtactgtccg acctgatcgg cgagcagcag
    11461 gcccttgtag acgacggtgc gcgtgctcat cgacggcacg aagtactcgg agccgtgcgt
    11521 gagcttcagg gcctgaatgc ggtgggaggc cgtcttgcgg atgacgtaga gcttgcgctc
    11581 gagcgcggtc tggaccatca cgtcgggccc gcggccgatg aagatctggc ggatgacggg
    11641 cgcgctcgcc ttcacggtcg gactcatcgt catcacttcg cagacgggaa cgtcgcgcca
    11701 gccgagcacg acctggcctt cgcgcctgac cgagcgctcg agctcctgtt cgcaggcggc
    11761 gcgggaggcg gcctcctgcg gcaggaagat catgccgacg ccgtagtcgc cgactgcggg
    11821 aagctcaacg ccctgcgcac gcatctcctc gcggtagaag gcgtccggaa tctgaatgag
    11881 aatgcccgcg ccgtcgccgc agagcggatc ggcgcccacg gcgcctcgat ggtcgaggtt
    11941 gtgcagaatc agcagcgcat cttcaatgac cttgtgcgac gccctgccct tgatgttgac
    12001 gacggcaccc acgccgcacg cgtcgtgctc gagttccgag cgataaagcc ctttgaggcc
    12061 ggcttcgcgc ctggcctcct ctttctgtcg ggtcataacc tggctctcca gtgtttctat
    12121 ttttggggga ttctgttttc catccgggaa agggagcgcc cgacgggccg gcggagagga
    12181 aacatggaga tgttgcatca aaaaatctcc gtgcgcatgc ctgcggcaca gagatttttg
    12241 aaaaacccgc aaatacggga tggcaaactg cctaggtcgg gcagtatgtc ttaggagata
    12301 ccgcctagaa actcacttcg tctatgcaaa ttaccttagt gtatcaccag aggctttcga
    12361 cagcattttc ggatgcgatc gggacctgaa atgaaaataa cggcgggacg ggcgatgatc
    12421 cgaaagcgct ccgaaaggcc cgtcccctga ctcatcaccg gccggatttg gcggcgtatt
    12481 tccgggtcgc atccagtgcg aactgatttc aggccttgtt gagggcgggc agttccgtct
    12541 tgtccagatc tttttcgcgc agctccgacc agcaggcgag ccacaggagc ttcgcgctaa
    12601 ggctgcccgt caaggatgaa aaaggtccag cgacacggcc gcaggaagca cggagtcacc
    12661 agatgacggt tcttgagcct ccaacggcaa aagtgtttac cgtcgtcatc agagctccta
    12721 cgacttgaac acgacggcga tgctttcgga acggctcccg caagcgtcat acgcctcaaa
    12781 aacagccatc ctgctctccc agcccgcacc tgccaggtcc cacgggcgaa gtcgagctcg
    12841 cctgaggagc tcctcggccg tctcctccgc ccgaccgcct gcatacggcg cgagcagcag
    12901 gcattcgccg gtcctttcaa gcgccagtgc cttgacgacg cgggtccagt cgagtctgcc
    12961 ccacgacagt ctcaccatct ccatcacgag aagaagtgcc tctcgccatc cttcataggc
    13021 ggagtcgcgc gaaagacgat cggcgcactc cttgacgatg tccgcctcgg cgaggcggtc
    13081 gggattcacc tgcgaaggat cctcgagtgc caacgccata aagtcgtcga aacgcttcat
    13141 catgctgccg tcaccgtccg tcatgaacgc gtcggcccag tagacaagtc cgttgcagag
    13201 cagaggctcc agttcctggc ccgtgatgta cttcttgtaa tcatctgcct cagtgtctag
    13261 cgtctgcctc acggcggcaa ggagaaccgc gctccagaca agtgttctga agaacggaac
    13321 acccgcgcgc ggcatcgagg aaagcgtcgg aaacgtggcc tcgccctgag taccgcccct
    13381 tgagtccgac attcttttcc ttgcaagagc accgccgccg ctcatgaagt gcctgtagtc
    13441 ggcaggatca ttccaatggg cccagaacgt ccagagcccg cggacaatga gcatggacgg
    13501 aagcagcacg cgcacgacgg catcgcttcc ctgaaaggtt ccccacggtc tttccttggc
    13561 cagattcgtc ccgagagccg cgtacttttc gagctcagcc tcaggcacaa acctgaggtt
    13621 cagcgacttg acgaggtcga cgagctgcat cgatgcacca cggttgtttt caccctggaa
    13681 atactgtgcg gcctcaatgt cgcttcgcaa aacggtggcg ctgaaaaaga catggtcgag
    13741 catccagacg acgcgttgcg catccgctct cacgtcaagc tggcctgcac ccgccttcct
    13801 gttgaggccg aacctgttgc gcaggcgctc tgtcatgagc atgatggcat cgaccgtagc
    13861 gggacgatcg tagctgaacc gtgcggcgcc ttcggcggcg cttcgcagcc tttcggcctg
    13921 ttcttctgaa agtccgaggt ttgcaccgtc cgcctgaagg cgttcggc
//
LOCUS       DBMDTI010000027        13808 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1370_length_13808_cov_4.220179, whole genome shotgun sequence.
ACCESSION   DBMDTI010000027 DBMDTI010000000
VERSION     DBMDTI010000027.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13808)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1370_length_13808_cov_4.220179"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            745..1926
                     /gene="metK"
                     /locus_tag="ACZPB6_02685"
     CDS             745..1926
                     /gene="metK"
                     /locus_tag="ACZPB6_02685"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR1183420.1"
                     /translation="MASEYLFTSESVSEGHPDKVADQISDAVLDACLEQDPMSRVAAE
                     TLCTTGLVVLAGEITTGANVDYIGLTRDVLKRIGYDNTEYGIDHKGCSVLVGYDKQSQ
                     DIAQGVDSAADDELNLGAGDQGLMFGYACDETPTLMPAPIYYAHRLVERQSIVRKNGS
                     FPTLRPDAKSQVTLRYRDGKPIGCDTIVLSTQHAPEMSEGKHMKAEFIDEVIEKIIRP
                     VMPAEWLKDTRFLINPTGRFVVGGPQGDCGLTGRKIIVDTYGGYCPHGGGAFSGKDPT
                     KVDRSAAYACRYVAKNIVAAGLASQCQVQVAYAIGVAEPMNITVFTNGTGVIPDEQIA
                     KLVRAHFDLRPRGIIQMLDLRRPIYSKTAAYGHFGREEPEFTWEKTDKAATLRDAAGL
                     H"
     gene            2077..3375
                     /locus_tag="ACZPB6_02690"
     CDS             2077..3375
                     /locus_tag="ACZPB6_02690"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR1183421.1"
                     /translation="MKKRVLTGINTTGTLHIGNYVGAIRPAIQDSKNPDVQSFFFMAD
                     LHALIKCQDPDRIERSRMQIAATWLAAGLDPERVVFYRQSDIPEIPLLNWMLTCCTSK
                     GQMNRAHAYKAATDAAVAAGKDPDADVSMGLFCYPILMAADILMFNANEVPVGHDQIQ
                     HLEMARDVATRFNNLYAKPDESFFVLPQATGGSAIEVLPGLDGRKMSKSYNNVIPLFE
                     GGRKALDEAISRIVTDSRLPGEPKDPDSTSLTVIYDAFATEEESAAFRQELRDGLGWG
                     EAKKRLADKIDAEIGPMRQRYEDLMAHPEQIEAILQQGAARARVYATDLIAKLRYAVG
                     LRSFQKLETASATKAQKKKAPATLFKQYRESDGLFYFKLTHEGSELLVSDGLESGRDA
                     GQWVGRLKKDPASLAEAPVHFAEGVAADNVVALLKSLAED"
     gene            complement(3419..4006)
                     /locus_tag="ACZPB6_02695"
     CDS             complement(3419..4006)
                     /locus_tag="ACZPB6_02695"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR1183422.1"
                     /translation="MTEETTAMLTKKGLRASLLARRKQLFESLALDNGLAERFLVMLE
                     LARPKSVGLFWPIRSEPGLLSVARDWLKRTGGLIYLPETRPDCMIYRLWHEGAELRKD
                     CAGIPYPQGEEASEPPAMVVAPCVGYAESCRRLGYGGGYFDRYLASYGNQRPLAVGVA
                     FDELRVPEEIFESFDEPLDAVVTERRIILKAKAPL"
     gene            4054..6099
                     /locus_tag="ACZPB6_02700"
     CDS             4054..6099
                     /locus_tag="ACZPB6_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013618.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR1183423.1"
                     /translation="MSSDAFNPKLLLSSLFLTGYLACMTPGFATEAPQTPTEQEAALL
                     LAADAEANKRFNEAWQAYRKGRIATLENLGATLEAHPLGDYPKLWQLLLEFRRNKDDP
                     DTNLRFIKFIERHQGQYLGEQSASDYLMTAADRINPVLFNRLYSLLQWNQEEPDILAW
                     HHWYNFETTPRKTIEAFVRDSKVKGRPLRMLTDRLMEQNPSWAWSAVLIQLQNRRWQE
                     VRYVVEHAPDKTMPASAKTLTAILNNPQRWYKKNAKNFDKLPARVQIFFIMRMRGIDF
                     KMSQKLAEKLSPKLSSEWQSFLWGQLAYEAAVSQTGDGAALFSRAGNRVYKNPVVVQP
                     DLMAGWAARAYMRDGDWKNVEKAISRMSADARENETWTYWLARAHAEQGRPAKAKPLY
                     EAISKNRTFYGKLACDELGKPYAVETSSAAITEEEIRRWTSDPSIRRAIAFYQNNFYF
                     LGHAEWNWALRNQKPRELYAAATFAKQQGLFHRMINTGQKASKDFFEPELLYPTPHKE
                     LITSMSARHGLPDAWVYGLIRQESRFMSGVSSGVGAQGLMQVMPKTAKWLIRKLDLKE
                     LKGKPLVDPNTNVALGTAYLNMLREDFDGSFALSSAAYNAGPVRARIWRKTLEKPMEG
                     AVFIESIPYFETRDYVKNVLSNTFMYAHRLKEDLGSFKSFVGTVTPSDPTAKEADLP"
     gene            6132..7265
                     /locus_tag="ACZPB6_02705"
     CDS             6132..7265
                     /locus_tag="ACZPB6_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR1183424.1"
                     /translation="MQIYTVGGYVRDMLLRQAGHDVPAGGDRDWVVVGSTPEEMLEKG
                     FTPVGADFPVFLHPKTHEEYALARTERKTARGYHGFVFHTDPGVTLEEDLKRRDLTIN
                     AMAMDDSGRIVDPYGGMEDLRNHVLRHVSEAFSEDPVRILRVARFSAKLCDFRIAEET
                     MALMTLMVENGEADALVPERIWAEVSRGFASRHPLNMIDALSRCGLWTKLFPVDPDNS
                     AMREVLSKAVSEDLCLDARFALMTAFCRNAEEAQQTAANLRTPVALQQLCSCFRTLLP
                     LFAEAVDAQTAGALLERGDALRRPERMALMIRMWEVLSQKNVEKLLKASELWCGVNAG
                     AVAKAQTNPRLIPAAVRAARIEALQPLFAASEKAGNHGHDGSQ"
     gene            complement(7224..7415)
                     /locus_tag="ACZPB6_02710"
     CDS             complement(7224..7415)
                     /locus_tag="ACZPB6_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR1183425.1"
                     /translation="MRWIICIFVLLVVVLSCVPTLRRWGLGRLPGDINFTVMGRKVEI
                     PLMSTILLTAIVAVVARLL"
     gene            7530..7868
                     /locus_tag="ACZPB6_02715"
     CDS             7530..7868
                     /locus_tag="ACZPB6_02715"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR1183426.1"
                     /translation="MLDCHIGAYEHEKHRTQKVTFECDVWIPLQDSTSSCDELQDVLN
                     YDLIVGTISDIAQSAHFNLQETLVDAIADKLATLPGVQLLRVSSAKTEAYEHVDAVGI
                     EVWRRRPSLP"
     gene            7873..8820
                     /gene="ttcA"
                     /locus_tag="ACZPB6_02720"
     CDS             7873..8820
                     /gene="ttcA"
                     /locus_tag="ACZPB6_02720"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HIR1183427.1"
                     /translation="MNNDSKNSIPIIPVSDVPSRPLNEDNEIFQNSKDGVNMGPSNRN
                     KKSVAVKKLEKRIIRLTAQAITDFGMIEDGDRVMVAMSGGKDSFVLLDALRTLQKRAP
                     IKFDLVAVNVDQHIPNFPREALEQYFKDIDVEYHIEDQDTYSIIQRLIPEGRNVCSLC
                     ARLRRGILYRVAGEMKCNKIALGHHMDDIVGTLLLNLFYGGRMKAMPPILRSDDKRNV
                     VIRPLAYVRESETEKFAKIRQYPVTSKGICGAGENLKRQEVKELIKAWDREFPGRIYN
                     LFMSLQRVSPSHLMDKRLYDFHNFVPLLPAASADEGEDE"
     gene            8929..9375
                     /locus_tag="ACZPB6_02725"
     CDS             8929..9375
                     /locus_tag="ACZPB6_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR1183428.1"
                     /translation="MKVIDMAVHEVATITADKTICDCARQMRLEHVGSLVVVNDDQTP
                     LGMITDRDIAIEVVARNLDPMKLTVRDVMTTPVVTAGPSESMVVALARMREFGVRRLP
                     IVDEEGKLVGVISNSNLVEELSSLLDSLVRNIHSSKTREVALRSDE"
     gene            complement(9632..10531)
                     /gene="rsmI"
                     /locus_tag="ACZPB6_02730"
     CDS             complement(9632..10531)
                     /gene="rsmI"
                     /locus_tag="ACZPB6_02730"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR1183429.1"
                     /translation="MNSLNDLWTSNALIQAAGLPGQNCPDSTLYIVGLPIGNLGDITL
                     RALWILALADVVAAEDTRETRKLLDKFGISVKLLSVREHNERHGAEQIIELLQEGKKV
                     ALVTDAGTPCVSDPGARAVKAVREAGFRVIPVPGASAVVTALSGAGLDGSGFAFVGFV
                     PPQSKARRNALAYWLARPEPFILYEAPHRVVDLLEDLAAAMEPERRVVVAREITKKFE
                     TFSSLTGAELAEWAALHEPRGEYVILVDEAPAKDNALSEDDIRWLRAMMSELPSSKLA
                     AVAAKVTGKPRDEIYQWLCEHKN"
     gene            10638..11984
                     /locus_tag="ACZPB6_02735"
     CDS             10638..11984
                     /locus_tag="ACZPB6_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016251.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HIR1183430.1"
                     /translation="MVVLPLGPAYATETADTRVPAEPVESTAPVMTKGEATRPVTIAM
                     LMPSDESPFLPAARIVGNGLLTASRVSGRNANIMLIEAPESVPINEMIDTAVFSGADV
                     VVGPLQKDQVEQLSREKNLPIPIVTLNYAATPDEEISPNMLMLSVATDLEAEYIARQA
                     VKALPEETAAGLPPKVLILTTDKPWEKRLSEAYMKVLNLSGVQYEVFTVTMDNLQDLQ
                     DKCRPELSQEERYKFNRMAATADSERARKQVHEAQRAHAAVAAPPYHSVLLALDARDA
                     GLVRSRLPLRTSVWATSTTNPGDPKTSSSASALAFDLNHVVFAECPFILRYDNESFEA
                     KFQTALPYSMPAKRLFALGLDAYQVAEDWAHGRKAFEISGETGQLTVHRDLSAQVVRT
                     PASFEIRSGELVDTAVDSEVPDEMAAEGEVEPNMGAPEPIEPEVLQEDDTTQNSNI"
     gene            12001..>13808
                     /locus_tag="ACZPB6_02740"
     CDS             12001..>13808
                     /locus_tag="ACZPB6_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1183431.1"
                     /translation="MPLITLMNAHAAWGDLPLLDDADLSIEPGERVGLIGRNGTGKST
                     LLSILAGHTHLDDGELRVQDGLRVLYVEQEPVLPEAANVKESLILRGKLDKIEDEREH
                     WRRLAKLDECLMKFELDPERSVARVSGGERKRAALALAFALEPDLLLLDEPTNHLDIV
                     AITTLENLVNAEFRTQRSLVVITHDRQFLDNVSSRIIELDRGVLRSYPGKFSEYESRK
                     QEELQAEELANRRFDKFWAQEEVWIRKGIEARRTRNEGRVRRLEELRRQRAARRDRMG
                     SVNLKIDAGEKSGKIVCETTDLCKSFDERSIVSNLNFKLMRGDRLGLIGHNGVGKSTL
                     IKLLLGKIEPDSGSIKLGTNLQVAYFDQLREQLDLSKTVAETISPGSEWVEIGGQKKH
                     IIAYMGDFLFPPRRVNVPVSSLSGGERNRLLLARLFALPANLLVLDEPTNDLDIDSLE
                     MLEQTLATYPGTIILVSHDRRFLDNVVTEVLAPEGNGLWREYVGGYSDWLQQRPEPVE
                     PAAPAAPQPKAAAKPRPRDNKIRMSYKETQELERLPKLIEDLEAEQAALMEAMSAPDY
                     HTKSVEELRKDAERAKAIEDEIASSYERWEALEEKR"
BASE COUNT         3059 a         4503 c         3646 g         2600 t
ORIGIN      
        1 gcaaaaaacg cagagggagc tttgaaaaaa gcttcagcaa ccagtccatg tcttgcgtcc
       61 attcgagaga agtaatccaa acaattaatt ttatcggatt tagtcccagt cccgacatga
      121 acccaatggg cctccaccgc ccatgcagcc atcatcaccc agtgcagact ttccatgaaa
      181 tcagtgacga caagttcatc tcgcccgagg cacggatgaa catcaacttc gtctcacagg
      241 gactccgccg cacggcggaa cccgattctc aagcacgtga acaggtcgaa cccaagcaca
      301 tgagccgtcg tccttcgaca cgatcacgcc accgggacta ggctgcgagg cggtgcccga
      361 ttcccaaaaa cgtgacggcc tgaaccagat atagagtgcg aagctcgccc ggatgtacct
      421 tgactgtcgg gaacatacaa tcgggcaacc tccacgagga gagtattcct gcaagcagag
      481 aaaagaacct gacgaccttc agccagacaa aagaaaacag attcacaata aggacacgtc
      541 acgaacgatg ccacgctcag attcattcgt gccttcagtg gcacggaaat cctgatggtt
      601 gtattattcg acgttcgccg agttaacaaa gacaacttgc ggggcgatga caaataccgc
      661 taaagcgtct tgcgcaatcg actggcagtc gacatacctc attgcgttag gcgctgtcct
      721 tctcattcct aatggagtat ggcaatggcc agcgaatatc tttttacttc cgaatccgtc
      781 agtgaaggtc atcccgacaa ggtcgcggac cagatctctg acgccgtcct cgacgcctgt
      841 ctcgaacagg atcccatgag ccgcgttgcc gcggaaaccc tctgcaccac gggcctcgtc
      901 gttctcgctg gtgaaatcac cacgggtgcc aacgtcgatt acatcggcct cacccgcgac
      961 gttctcaagc gcatcggcta tgacaacacc gaatacggca tcgaccataa gggttgctcc
     1021 gtcctcgttg gttacgacaa gcagtctcag gacatcgctc agggtgtcga cagcgccgcc
     1081 gacgacgaac tcaacctcgg tgctggcgac cagggcctga tgttcggcta cgcctgcgac
     1141 gaaacgccga ccctcatgcc cgcaccgatc tactacgccc accgtctcgt cgagcgtcag
     1201 agcattgtgc gcaagaacgg ctccttcccg acgctgcgcc ctgacgccaa gagccaggtg
     1261 acgctccgct atcgcgacgg caagccgatc ggttgcgaca cgatcgtgct ctccacgcag
     1321 catgcccctg aaatgtccga gggcaagcac atgaaggctg agttcattga cgaagtcatt
     1381 gaaaagatca tccgtcctgt catgcctgcc gaatggctca aggacacccg cttcctcatc
     1441 aacccgaccg gccgcttcgt ggttggcggt ccgcagggtg actgcggtct gaccggtcgc
     1501 aagatcatcg tcgacacgta cggcggctac tgcccgcacg gcggcggcgc cttctctggc
     1561 aaggatccga cgaaggttga ccgctccgca gcctatgcct gccgctatgt cgccaagaac
     1621 atcgttgccg ccggtctcgc ctctcagtgt caggttcagg tcgcctacgc catcggcgtt
     1681 gccgaaccga tgaacatcac cgtgttcacg aacggcaccg gcgtgatccc cgacgaacag
     1741 atcgccaagc tcgtgcgcgc tcacttcgac ctgcgtccgc gcggcatcat ccagatgctc
     1801 gacctgcgcc gtccgatcta ctcgaagacc gccgcctacg gccacttcgg tcgcgaagaa
     1861 ccggaattca cgtgggaaaa gaccgacaag gccgccaccc tgcgcgatgc cgctggtctc
     1921 cactaatcag ctgactccgc aatactgaaa accggcctgc agaattctgc aggtcgtttt
     1981 tttattagca gacaaacgcg tatcattgaa agactgcctt gacggcgccc tcccgagagg
     2041 cgcgtccgtc caaccaacct gatggaacac acagacatga aaaagcgagt tctgaccggc
     2101 atcaacacga ccggcaccct ccacatcggc aattacgtcg gcgccattcg cccggccatc
     2161 caggacagca agaatccgga cgtgcagagc ttcttcttca tggctgacct tcacgccctc
     2221 atcaagtgcc aggaccctga ccgcatcgaa cgcagccgca tgcagatcgc cgctacgtgg
     2281 cttgccgcag gcctcgatcc tgaacgcgtc gtgttctatc gacagagcga cattcctgaa
     2341 attccgctcc tcaactggat gctgacctgc tgcacgagca agggtcagat gaaccgtgct
     2401 catgcctaca aggctgcaac cgacgctgcc gttgccgcag gcaaggatcc tgatgccgac
     2461 gtctcgatgg gcctgttctg ctaccccatc ctcatggccg ccgacatcct catgttcaac
     2521 gccaacgaag tgcccgtcgg tcacgaccag atccaacacc ttgaaatggc ccgtgacgtc
     2581 gccacgcgct tcaacaatct ctacgccaag cccgacgagt ccttctttgt gcttccgcag
     2641 gccacgggcg gctctgccat tgaagtcctc cccggtctcg acggccgcaa gatgagcaag
     2701 tcctacaaca acgtgatccc gctctttgag ggcggccgca aggcgcttga cgaagcgatc
     2761 tcccgcatcg tcaccgacag ccgtctgccc ggtgagccca aggatcccga cagcacctcg
     2821 ctcaccgtca tttacgacgc cttcgccacc gaagaagaat ccgccgcctt ccgtcaggaa
     2881 ctccgtgacg gtcttggctg gggtgaggcc aagaagcgcc tcgccgacaa gatcgacgcc
     2941 gaaatcggcc cgatgcgcca acgctacgaa gatctgatgg ctcatcccga acagatcgag
     3001 gccattcttc agcagggcgc agcccgtgcc cgcgtctacg caacggatct catcgccaag
     3061 ctccgttacg ctgtcggcct gcgctccttc cagaaactcg agacggctag tgccaccaag
     3121 gctcaaaaga agaaggcgcc tgccacgctc ttcaagcagt accgtgaaag cgacggcctc
     3181 ttctacttca agctcacgca cgaaggctcc gaactgctcg tttccgacgg cctcgaatcc
     3241 ggccgcgacg caggccagtg ggtcggccgc ctcaagaagg accccgcctc tctcgccgag
     3301 gctcctgttc acttcgctga aggcgttgcc gccgacaacg ttgtcgccct tctgaagtct
     3361 cttgccgaag actaagctcc gtccttgagc ttcgacgcct caagcgccgc cgtgcatgtc
     3421 acagcggcgc ttttgctttt aggatgatcc ttcgctccgt tacaactgca tcaagcggct
     3481 cgtcaaatga ctcgaagatc tcttcgggca cccgaagctc atcaaaggca actccaaccg
     3541 ccagtggacg ctgattgccg taggaggcca gatacctgtc gaaatagcct ccgccatacc
     3601 cgagccgcct gcacgactcc gcataaccca cgcacggcgc cacaaccatg gcaggaggct
     3661 cggaagcctc ctctccctgc gggtacggaa tgcccgcgca gtcctttcga agctcagcac
     3721 cttcatgcca aagcctgtag atcatgcaat ccggacgagt ttcaggcagg tagatcagtc
     3781 cgcccgtccg cttcagccaa tcccgagcaa cactcagcaa tccgggctcc gaacggatcg
     3841 gccagaaaag cccgacgctt ttgggacgag caagttccag cattaccaaa aaccgctctg
     3901 caagcccgtt gtcgagcgca agagattcaa acaattgttt ccgtctcgcc aaaagagaag
     3961 cgcggaggcc tttcttggtt aacattgctg tagtctcttc tgtcatttat acgtctcctt
     4021 ccctttgttc cgctttttac ggagctcttg aaaatgtcgt ctgatgcctt caatccgaaa
     4081 cttctgcttt caagcttgtt cctgacggga tatcttgcct gcatgactcc cggatttgca
     4141 accgaagctc cgcagacacc gaccgaacag gaagcggcgc tccttctggc tgccgatgca
     4201 gaagccaaca agcgcttcaa tgaggcctgg caggcttaca ggaagggcag gattgctacg
     4261 cttgaaaacc ttggcgccac gcttgaggct caccccttgg gcgactatcc gaaactctgg
     4321 cagcttctgc tcgaattccg tcgcaacaaa gacgatccgg acaccaacct gcgcttcatc
     4381 aagttcatcg agcgccatca ggggcagtac cttggagaac aatccgcatc cgactatctg
     4441 atgaccgctg ccgatcgaat caatccggtg ctcttcaatc gcctctacag cctgctgcaa
     4501 tggaaccagg aagagccgga cattcttgcc tggcaccact ggtacaactt cgagactact
     4561 ccccgaaaga cgatcgaagc ctttgtgcgt gactccaagg tgaagggccg cccgcttcga
     4621 atgctgaccg accgcctgat ggaacagaat ccgtcgtggg cctggtccgc cgttttgatc
     4681 cagctgcaga accgtcgctg gcaggaggtg cgctacgtcg tcgaacatgc gcccgacaag
     4741 accatgcccg cctcggcaaa gactctgacc gcgatcctga acaatccgca gcgctggtac
     4801 aagaagaacg ccaagaactt cgacaagctt cccgcacgtg tgcagatctt cttcatcatg
     4861 cgtatgcgag gcatcgactt caagatgtcg cagaagctcg cagaaaagct ctctcccaag
     4921 ctctcctctg aatggcagag cttcctctgg ggacagctcg cctacgaagc cgccgtcagc
     4981 cagacgggcg acggtgccgc tctcttctct cgtgcaggga atcgcgtcta caagaacccg
     5041 gtcgtcgttc agcccgacct gatggcagga tgggccgctc gcgcctacat gcgtgacgga
     5101 gactggaaaa acgtcgagaa ggccattagc cgcatgtccg ccgatgcccg cgaaaacgag
     5161 acctggacct actggcttgc gcgtgctcat gccgaacaag gccgtccagc caaggccaag
     5221 ccgctttacg aggccatctc gaaaaaccga accttctacg gaaagcttgc ctgtgacgaa
     5281 ctcggcaagc cctatgcagt tgaaacctct tccgcagcaa tcactgaaga ggaaatccgt
     5341 cgctggacct ccgatccgag catccgaaga gccatcgcct tttaccagaa caacttctat
     5401 ttccttggac acgccgaatg gaactgggct ctacgcaatc agaagcccag ggagctctat
     5461 gccgccgcaa catttgcgaa gcagcaaggt ctcttccacc gcatgatcaa cacgggacag
     5521 aaggccagca aggacttctt tgaacccgag ctgctctatc ccacgcctca caaggagctc
     5581 atcacgtcga tgagcgcccg tcacggtctt cccgacgcct gggtctacgg cctcattcgt
     5641 caggaatccc gcttcatgtc cggcgtaagt tccggcgttg gcgctcaggg gctcatgcag
     5701 gttatgccga aaaccgccaa gtggctcatc cgcaagctcg atctcaaaga actcaaggga
     5761 aagccactcg tcgatcccaa caccaacgtt gcactgggca ccgcctacct gaacatgctt
     5821 cgcgaggact tcgacggtag cttcgcgctt tccagcgctg cctacaacgc aggtcccgtc
     5881 cgtgcgcgca tctggcgaaa gacgctggaa aagcccatgg agggcgccgt cttcatcgag
     5941 tccatcccct atttcgaaac ccgcgactac gtaaagaacg tgctctcgaa cacattcatg
     6001 tatgctcatc ggctgaagga ggatctcggc tccttcaagt cgttcgtggg cacggtgacg
     6061 ccttcggacc cgacggccaa ggaggccgat cttccctgat cgggattttc tgaccggaca
     6121 agcttcaact tatgcagatt tataccgtcg gcggatacgt gcgtgacatg cttcttcgtc
     6181 aggcgggcca tgacgtgccc gcaggcggag accgtgactg ggtcgtcgtg ggctccacgc
     6241 ccgaggaaat gcttgaaaag ggcttcaccc ccgtcggcgc ggacttccct gtatttcttc
     6301 acccgaagac gcatgaggaa tatgcgctcg cccgtactga gcgaaaaacc gcccgcggct
     6361 accatgggtt tgtatttcac acagatcccg gcgtgacgct cgaggaggac ctcaagcggc
     6421 gtgacctgac gatcaacgcc atggccatgg acgacagcgg tcgaatcgtc gacccgtacg
     6481 gcggcatgga ggatcttcgg aaccacgttc ttcgtcacgt aagcgaagca ttttccgagg
     6541 atcccgtccg cattcttcgc gtagcccgct tcagcgccaa actctgcgac ttccgcatcg
     6601 ccgaggaaac catggcgctc atgacgctca tggtcgagaa cggcgaggcg gatgcactgg
     6661 tgcccgaacg aatctgggcg gaagtgagcc gcggctttgc atcgcgccat cccctcaaca
     6721 tgattgacgc gctttcccgc tgcggcttgt ggacgaagct ctttcctgta gacccggaca
     6781 actccgccat gcgcgaagtc ctctccaaag ccgtgtctga agacctgtgc cttgatgcgc
     6841 gctttgccct catgacggcc ttctgccgaa atgcagaaga agctcagcaa acggcggcca
     6901 accttcgaac ccccgtcgcc ctgcagcagc tttgcagctg cttccgcacc ttgcttccgc
     6961 tctttgccga agcagttgat gctcagactg caggggctct gcttgagcgc ggtgatgcac
     7021 ttcgcagacc tgaacgcatg gccctcatga tccgcatgtg ggaagtgctg tcccaaaaaa
     7081 acgtggagaa actcctgaag gcgtcagagc tttggtgcgg cgtgaacgcg ggggccgttg
     7141 ccaaggctca gaccaacccc agactcattc ccgccgccgt gcgtgccgcc cgcattgaag
     7201 cgctgcagcc gctctttgcc gcctcagaga aggcgggcaa ccacggccac gatggcagtc
     7261 agtaaaatcg tgctcatcaa ggggatctca accttgcggc ccatgaccgt gaagttgatg
     7321 tccccgggaa ggcgtccaag cccccatcgt ctgagcgtcg gcacgcacga gagcacgacg
     7381 acaagcaaga cgaaaataca gataatccat cgcatcagcg cgtttccttt cagctcaagc
     7441 tgcctacaac ggccggccgt tgcaccatga acagccatat tacagacttt ctgaccttcc
     7501 aatgtcgcaa gattttcgtt cgaggccttg tgcttgactg ccacatcggc gcatacgagc
     7561 atgaaaagca ccgcacccag aaggtcacct ttgaatgcga cgtctggatt ccgcttcagg
     7621 actccacgag cagctgcgat gaacttcagg atgtgctcaa ctacgacctg atcgtcggca
     7681 ccatctcgga catcgcacag tccgctcact tcaacctgca agaaactctc gtggacgcca
     7741 ttgccgacaa gctggcgaca ctgcccggcg tacagttgct ccgcgtttcc tccgccaaga
     7801 ccgaagccta cgaacatgtc gacgccgtcg gcatcgaagt ctggcgccga cgcccctcac
     7861 tcccttaaga tcatgaacaa cgacagcaag aacagcatcc ccatcatccc cgtctccgac
     7921 gtcccctctc gtcctctcaa cgaagacaac gagatcttcc agaacagcaa ggacggcgtc
     7981 aacatgggtc ccagcaaccg caataagaag tcggttgccg tcaaaaagct tgagaagcga
     8041 atcatccgcc tcacagccca ggccatcacc gacttcggca tgattgagga cggtgaccgc
     8101 gtcatggtcg ccatgagcgg cggcaaggac agcttcgtgc ttctcgatgc cctccggacg
     8161 ctccagaagc gcgcgccgat caagttcgat ctcgttgccg tcaatgtcga ccagcacatc
     8221 cccaacttcc cccgtgaagc actcgagcag tacttcaagg acatcgacgt cgaataccac
     8281 atcgaggatc aggacaccta ctccatcatc cagcgcctga tccctgaagg ccgcaacgtc
     8341 tgctcgctct gcgcccgtct ccgccgcggc attctctacc gcgtcgcagg cgaaatgaag
     8401 tgcaacaaga tcgccctcgg ccatcacatg gacgacattg tcggcacgct gctgctcaat
     8461 ctcttctacg gcggccgcat gaaggccatg ccgcccattc tgcgaagcga cgacaagcgc
     8521 aacgtcgtca tccgcccgct tgcctacgta cgcgaaagcg aaacggagaa gtttgccaag
     8581 atccgccagt accccgtcac ctcaaagggc atctgcggag cgggtgaaaa cctcaagcgt
     8641 caggaagtga aggaactcat caaggcctgg gaccgtgagt tccccggccg catctacaac
     8701 ctcttcatga gcctgcaacg cgtctctccg tcgcacttga tggacaagcg cctgtacgac
     8761 tttcacaact ttgtcccact tctgcctgcg gcttctgccg acgagggtga ggacgaataa
     8821 attcaagccc tcctccaagc cgagcaccaa ccgtttggtt agaatttatt cattacgcaa
     8881 agcagaaagc gtcccaggaa aaaggtagaa aacatgagaa ctcgctctat gaaagtcatc
     8941 gacatggccg tgcatgaggt cgccacgatc acggctgaca agaccatctg cgactgtgca
     9001 aggcagatgc gccttgagca tgtcggcagc cttgttgttg tcaatgacga tcagacgccc
     9061 ctcggcatga tcacggaccg cgacatcgcc atcgaagtcg tcgcccgcaa tctggatccg
     9121 atgaagctca ccgtccgcga cgtcatgacg actcccgtcg tcactgcggg ccccagcgaa
     9181 agcatggtcg tggcacttgc ccgcatgcgt gaattcggcg tccgccgcct gcccatcgtt
     9241 gatgaggaag gcaagctcgt cggcgtgatc tccaactcca atctcgtcga agagctctcc
     9301 tcgctgctcg acagcctggt gcgcaacatt cactcctcga agactcgaga agtcgccctt
     9361 cgctctgacg aatgatgttc ccgtggaaca tcatcgaaga tgatccctgt cggttccgaa
     9421 cgttgttcgg aaccgttttt tttttcgatc caggatgttt cacatggaac aatcctgcgt
     9481 gaatcctgca gaagtctccc tctccttcat tttccatgac gatgttccac gtggaacatt
     9541 atcctgagag cagaccgcag gcatgaaaaa acgctcagcg aagaatacct tcacggaccg
     9601 tttccttaag gacggggcga attgaagccg atcagttctt gtgctcgcaa agccactggt
     9661 agatttcatc acgcggcttg cccgtcacct tggcggcgac ggcggccagc ttcgaactcg
     9721 gaagctcgct catcattgca cgaagccatc ggatgtcatc ctcggagagg gcattgtcct
     9781 ttgcgggcgc ctcgtccaca agaatgacgt actccccgcg gggctcgtgc aaagcggccc
     9841 attccgcgag ttcggcccct gtaagactgg agaaggtctc gaacttcttc gtgatctcac
     9901 gtgccaccac gactctgcgc tcgggctcca tggccgcagc cagatcttca agcagatcca
     9961 cgacacgatg cggcgcctca tagagaataa agggctcagg acgtgccagc cagtaggcaa
    10021 gcgcattgcg gcgcgccttg gactgcggcg ggacgaaacc cacgaacgca aagcccgagc
    10081 cgtcgaggcc ggccccggaa agcgccgtca cgacggcaga ggcgccggga acgggaatga
    10141 cgcggaaccc tgcctcgcga acggccttga ccgcacgggc gcccggatcg gacacgcagg
    10201 gcgtgcccgc atcggtaacg agcgccacct ttttgccttc ctgaagaagc tcgatgatct
    10261 gctcggcacc atgcctttcg ttgtgctccc taaccgacag aagttttaca ctaatgccaa
    10321 atttatccag aagcttgcgg gtttcgcgtg tatcctcagc ggcgacaaca tcagccagag
    10381 cgagtatcca gagcgcccgc agtgtaatat ctccaagatt gccgataggc agcccgacga
    10441 tgtaaagcgt gctgtcgggg cagttctgcc caggcaggcc cgccgcctga atcaaagcgt
    10501 tagatgtcca taaatcattg agggaattca tcgtgtccgt ccttccattt gcccgacagt
    10561 tgttcagaac tttaatcata agcttaccga ttgtaagcgt tgctgcgacg acttcggttg
    10621 atgctacgga ttccggggtg gtcgtccttc ctctgggccc tgcatacgcc accgaaacgg
    10681 ccgacacccg ggttcctgcc gaacccgtcg aaagcacggc acccgtcatg acaaaaggcg
    10741 aggccacccg ccccgtcacc atcgccatgc tgatgccgtc cgacgaatct ccgttccttc
    10801 ccgccgcacg cattgtgggc aacggtctgc ttacggcaag ccgtgtcagc ggccgcaacg
    10861 ccaacatcat gttgatcgaa gcgcctgaaa gcgttcccat caacgagatg atagacaccg
    10921 ccgtcttctc cggcgccgac gtcgtcgtcg gaccgctcca gaaggatcag gtggaacagc
    10981 tctccaggga aaagaatctt ccgattccca tcgtaaccct caactatgcc gcgacgcccg
    11041 acgaagagat ttcgcccaac atgctgatgc tctccgtcgc gactgacctt gaagccgagt
    11101 acatcgcacg tcaggctgtg aaggcgctgc ctgaagagac agccgcaggc ctgcccccga
    11161 aggtgctcat cctcacgacg gacaagccgt gggaaaagcg cctgagcgaa gcgtacatga
    11221 aggttctgaa tctttctggc gtccagtatg aggtcttcac ggtcacgatg gataacctgc
    11281 aggatctcca ggacaagtgc cgtcccgaac tgagccagga ggaacgctac aagttcaacc
    11341 gcatggctgc aacggccgac agcgaaagag cccgcaagca ggtccacgag gcgcagcgtg
    11401 cccacgcggc cgttgccgcc cccccgtatc actccgtcct gctcgcactc gacgccaggg
    11461 atgccggtct ggttcgcagt cgtttaccct tacgcacaag cgtttgggcg acatcgacca
    11521 ccaatcccgg tgatccgaag acaagttcga gcgcttctgc gcttgccttt gacctgaacc
    11581 acgttgtttt tgcagaatgt ccctttattt tgcgctatga caacgagagc tttgaggcca
    11641 aatttcagac cgccctgccc tattccatgc ccgccaagcg tctcttcgca ctcgggcttg
    11701 acgcctatca ggttgctgaa gactgggctc atggcaggaa ggctttcgaa atcagcggcg
    11761 aaaccggcca gctgaccgtc caccgcgacc tcagcgccca ggtcgttcgc acccccgcct
    11821 ccttcgagat ccgctcaggc gagctggtcg acactgccgt cgattccgaa gtcccggatg
    11881 aaatggccgc cgaaggagaa gtggagccta acatgggagc gcctgaaccc atcgagcccg
    11941 aagtgcttca ggaagacgac acgacgcaga acagcaatat ttaaatagaa caaacggaaa
    12001 atgccgctta tcaccctgat gaacgcccac gccgcctggg gcgaccttcc gcttcttgac
    12061 gatgcggacc tctccatcga acccggcgaa cgcgtcggtc tgatcggccg caacggcacg
    12121 ggcaagagca ccctgctctc catccttgcg ggccacacgc atctcgacga cggcgaactg
    12181 cgcgtgcagg acggcctgcg cgtgctgtac gtcgagcagg agcccgtcct gcctgaggca
    12241 gccaatgtca aggaaagcct catcctgcgc gggaagcttg acaagatcga ggatgagcgc
    12301 gagcactggc gtcgtctggc caagctggac gagtgcctga tgaagttcga gctcgatccc
    12361 gagcgctcgg tcgcccgcgt gagcggcggc gagcgcaagc gtgcggctct ggcgctcgcc
    12421 tttgccctcg agccagatct tctgcttctc gacgagccga cgaaccatct ggacatcgtc
    12481 gccatcacga cgctcgaaaa cctcgtcaac gcggaattcc gcacccagcg ctcgcttgtc
    12541 gtcatcactc acgaccgtca gttcctcgac aacgtctcga gccgcatcat cgagcttgac
    12601 cgaggcgtcc tccgctccta tcccggcaag ttttccgaat acgaaagccg caagcaggaa
    12661 gagcttcagg ccgaagagct ggccaaccgc cgcttcgaca agttctgggc tcaggaggaa
    12721 gtctggatcc gcaagggcat cgaagcccgc cgcacccgca atgagggccg cgtgcgacgc
    12781 ctcgaagaac tccgccgaca gcgtgccgcc cgccgcgacc gcatgggcag cgtcaacctc
    12841 aagatcgacg caggcgagaa gagcggcaag atcgtctgcg aaacgacgga cctctgcaag
    12901 tcctttgacg aacgctcgat cgtcagcaac ctcaacttca agctcatgcg cggcgaccgc
    12961 ctcggcctga tcggtcacaa cggagtcggc aagagcacgc tgatcaagct cctgctcggc
    13021 aagatcgagc ctgattcggg ctccatcaag ctcggcacga acctgcaggt cgcttatttc
    13081 gaccaactga gggaacaact cgacttaagc aagaccgtcg ccgaaacgat ctccccaggt
    13141 tccgagtggg tggagatcgg cggccagaag aagcacatca tcgcctacat gggtgacttc
    13201 cttttcccgc cgcgacgcgt gaacgtgccc gtgagctcgc tctccggcgg cgaacgcaac
    13261 cgcctgctgc tcgcccgcct gttcgccctg cccgccaatc tcctcgtgct cgacgagccg
    13321 acgaacgacc tcgacatcga ctctctggag atgctcgaac agacgcttgc gacctacccc
    13381 ggcacgatca ttctcgtcag ccatgaccgc cgcttcctcg acaacgtcgt gacagaggtg
    13441 ctcgcccctg aaggcaacgg cctctggcgc gaatacgtcg gcggctattc ggactggctt
    13501 cagcaacgtc ccgaacccgt cgaacctgcc gcacccgccg ctccgcagcc caaggccgca
    13561 gccaagccgc gcccgcgcga caacaagatt cgaatgagct acaaggaaac gcaggagctc
    13621 gaacgcctgc ccaagctcat tgaagacctt gaggccgaac aggccgccct gatggaggcg
    13681 atgagcgccc ccgactacca cacgaagtcc gttgaggagc ttcgcaagga cgccgaacgc
    13741 gccaaggcga tcgaggatga aatcgcttcc agttacgagc gctgggaggc gctcgaggaa
    13801 aagcgcca
//
LOCUS       DBMDTI010000028        13761 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1377_length_13761_cov_4.258858, whole genome shotgun sequence.
ACCESSION   DBMDTI010000028 DBMDTI010000000
VERSION     DBMDTI010000028.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 13761)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13761)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13761
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1377_length_13761_cov_4.258858"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            325..2040
                     /locus_tag="ACZPB6_02745"
     CDS             325..2040
                     /locus_tag="ACZPB6_02745"
                     /inference="COORDINATES: protein motif:HMM:NF036516.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HIR1183432.1"
                     /translation="MFRYTVTPDPHAHLLRVSLELTPPQDAVTLHMPSWTSGSYLIRD
                     YAGRIRGPSSNTGTLVQTDKCHWTVSGLTPGATLRIEWEAFTYSVGIHDAWLDDIRGF
                     YNPAAVLLIPEGIDAPCLLDFSDCRFVPHCSLEPIGTHSFAASCTQELLDAPVTFTSA
                     KGEHCILNLSVCGIPHTLVFTGPGSSTLNKDLILQDAQAILKTVLDFWGAAPFRQYLF
                     HIQIGPRLYGGLEHEASCVLQTDTLALPGASESSRPKDYEDFLTVFAHEYFHAWLVKH
                     LRPASFLPYKLERECHTHDLWVFEGFTTYYENVLPFRAGVLDEDKLLSLTSARFNRVR
                     EREGFHVESLSDASFNAWTHLYKQTPDSAYCQASYYGKGAVLAFMLDAALRKAGFSLD
                     EVLKTWFHEALADPQARSLPDGGFFERIPVASIAERFKKLVTSSDRTLWENEWRCALS
                     TLGMSEEADKNTPASRMHLGLQLDGLRVRYAESRGSAYAAGLFAGDEIIAVNGLRADA
                     ATIERMIAQAKGSTVHIHYFREHLLRSAEIEIPVNPLPQPGTLKMKNPTEFGRAWIRS
                     SNRQA"
     gene            complement(2015..2728)
                     /locus_tag="ACZPB6_02750"
     CDS             complement(2015..2728)
                     /locus_tag="ACZPB6_02750"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR1183433.1"
                     /translation="MTASLFSEGVMPDMTLPADPLWHELVEAFMATDKGRRAVERVAE
                     DVAAGYQVYPPRVFRAMDETPSNGVRVVILGQDPYHGPGQAEGLSFSVPAGIKVPPSL
                     RNIKKELQRDLGVSVTQQGSLLSWAHQGVLLLNAILTVRGGEAASHRSLGWETLTDSI
                     IRAVSETAPHAVFMLWGNFAQGKRALIEEEKHLVLCANHPSPLSALRPPVPFIGCGHF
                     GAANTWLAEHGLRPVDWTI"
     gene            complement(2725..3156)
                     /locus_tag="ACZPB6_02755"
     CDS             complement(2725..3156)
                     /locus_tag="ACZPB6_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HIR1183434.1"
                     /translation="MDLKRILLCGLVLAAASFAAHAVSPMVEINMCYQKAGDDAVKIG
                     ACLEQESKLVQAEYKDAVERVTVVAKAWDKPNRNRTRWDKLMRANQAFDNFIKRECDF
                     VKNTTKGSSTQENNAALACRINLYRMRTDMLENRYLSSAKQ"
     gene            complement(3222..3911)
                     /locus_tag="ACZPB6_02760"
     CDS             complement(3222..3911)
                     /locus_tag="ACZPB6_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HIR1183435.1"
                     /translation="MRLVKAVLFDLDGTLVDSLPELYHGVHTVLSDMGLKAPSQEAVG
                     AMIGRGILVLVQRLCAALGIAVESEQGRDLFDRLLKAWSESGGRHMAFYPGVIEGVEQ
                     LKDKGIRVALVTNKQRSLTVEFLQSRGLESLFDTVVAGDDCPNNKPAPDMLIRAMTEM
                     NVCASETVMVGDSRNDALAGRAAGVSVALVETGYNEGVSIADWAREAGFTRVYPSARK
                     VCERIVASGRL"
     gene            complement(3915..4604)
                     /gene="rpe"
                     /locus_tag="ACZPB6_02765"
     CDS             complement(3915..4604)
                     /gene="rpe"
                     /locus_tag="ACZPB6_02765"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR1183436.1"
                     /translation="MRLCRIAPSILSADFARLGQEVSDVVEGGADWIHFDVMDNHYVP
                     NLTVGPLVLKAIRPYSKVPVDVHLMVEPVDELVPAFAKAGADIITFHCEASRHVDRTL
                     QLIRDHGMKSGLVFNPATPLTYLDYEFDRIDVVLLMSVNPGFGGQSFIGATLDKIRAV
                     REKLDRYEAETGRRIALEVDGGIKPANIAEVALAGADTFVAGSAVFGAGNAAGYADVI
                     SRMKAAVAALE"
     gene            4790..5965
                     /gene="mltA"
                     /locus_tag="ACZPB6_02770"
     CDS             4790..5965
                     /gene="mltA"
                     /locus_tag="ACZPB6_02770"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HIR1183437.1"
                     /translation="MLSRLIRLAAPAAALLLAACTTPEPPVKPVPPAHPEVAFEESSF
                     DRLPQIQDHTWEPALAAFQRSCEVMQAGPVWEDVCRSALATDPIGAQRFFLANFTPWK
                     VFVANDDATLVQDTGLMTGYYEPELLGSRTRSAPFTHPIYGVPDDLLVIDLAELYPQL
                     KELRLRGKLEGRRVVPYDDRATIQKRTDLAPRAIAWVNDPVDAFFLHIQGSGRIRLPD
                     GTFMRVGFADQNGRKYHSIGTWLIREGHLKSHELSMQRIRRWARENPSRVDEALAQNP
                     SYVFFEERKGSPDLGPLGAQGVPLTPEASVAVDLRYWRLGTPFIVDASQASPALKIVR
                     PVIAQDTGGAIRGIIRFDYFWGFGDKAGEAAGRQKSNTFSWVLVPNGMKPEDIMVRR"
     gene            complement(6043..6744)
                     /locus_tag="ACZPB6_02775"
     CDS             complement(6043..6744)
                     /locus_tag="ACZPB6_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003756 - protein disulfide isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HIR1183438.1"
                     /translation="MLKKSLFVALCAGLAVTVFAATPQSVEDALMQTTGLKADAVQKS
                     PVPGMWEVVVQDRVFYVDDQARYVLYGSLIDTVKQTNLTNERLRERSRERWKEWPFQD
                     AVKQVFGKGEREVVVFSDANCTYCCAMESVYAQVGNVTVYTFITPMLRGETNAREIVC
                     AKDRAKAWHEWMGNNVRPNSVLAGCDTSMLQRNLVLANRLNVTGAPTFFFKSGDRVTG
                     AMAASQFEKLVSTVE"
     gene            complement(6888..7283)
                     /locus_tag="ACZPB6_02780"
     CDS             complement(6888..7283)
                     /locus_tag="ACZPB6_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1183439.1"
                     /translation="MNLKNMTKSAEGTVKDPGRNLKQKSGLNRSLARVAPYGMRMAIQ
                     WALFKADGRLILVDPKYTSQTRPRCGYTSSTNRPAQAHFGCQLCGCTENADVAGALNV
                     LKKSRTGSVRPSSELGNKSAAGTVLFFAS"
     gene            7475..7550
                     /locus_tag="ACZPB6_02785"
     tRNA            7475..7550
                     /locus_tag="ACZPB6_02785"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7508..7510,aa:Phe,seq:gaa)
     gene            7589..7664
                     /locus_tag="ACZPB6_02790"
     tRNA            7589..7664
                     /locus_tag="ACZPB6_02790"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7622..7624,aa:Phe,seq:gaa)
     gene            complement(7805..8530)
                     /gene="queC"
                     /locus_tag="ACZPB6_02795"
     CDS             complement(7805..8530)
                     /gene="queC"
                     /locus_tag="ACZPB6_02795"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIR1183440.1"
                     /translation="MSETNAMQPARRGAAMVLFSGGQDSTTCLAWALSRFERVETLGF
                     DYGQRHAVELECRKTVLEKLRAFRPEWVQRLGEDHMLDMGLLGQISDCALTREQELRF
                     MENNIPNTFVPARNLLFFTFAAALAYRRGIETLVGGMCETDYSGYADCRDNTLKSLQV
                     SLSLGLDAPMVIETPLMWLDKAQTWMLAEALGGRELVDLIRLDTHTCYLGDHEHVHEW
                     GYGCGTCPACQLRAKGWERYCDR"
     gene            complement(8527..9048)
                     /locus_tag="ACZPB6_02800"
     CDS             complement(8527..9048)
                     /locus_tag="ACZPB6_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183441.1"
                     /translation="MRDEWADPAAGELALAARRAREEVKRRALLSAGAVLVPLPGLDV
                     AVDVGVLVSMMNAVNRAFCLSPDQIERLGMEERVAVYEALSGVGAVMAGKSVTGVAAL
                     TIAKQLGSRWCAGKTARWVPIVGQGAAAALSYGLVKWLGEQHVRECLAVRSRVAALLP
                     YEKDSLKKNAQQS"
     gene            9172..10461
                     /gene="hemA"
                     /locus_tag="ACZPB6_02805"
     CDS             9172..10461
                     /gene="hemA"
                     /locus_tag="ACZPB6_02805"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR1183442.1"
                     /translation="MHLLAIGLNHTTAPVSVRERVAFGPEEIAETIGHMRERFSSTQM
                     GGIHEAAILSTCNRTEIYCAAEDTDAARDSVLGFICERKNVSRSELEPHIYTFTQEEA
                     AKHTFRVASGLDSMVLGETQIVGQMKKAEKMARDAHGLGTMLNHLFQSTFTVAKEVRT
                     ATAIGANSVSLAAAAVRLALRLFGSLKDERVLFVGAGEMIELCAAHFAAQQPKSITIA
                     NRTLERGQVLARTVHADAIRLADLPEKIAGYDIIVSCTASALPIIGLGMIERAINERK
                     HRPMFIVDLAVPRDVEPEIDRLDDVYVYTVDDLGKVVNQGMASRQAAITQAETIISLR
                     MSDFAEWLRQRETVPFIQSLRDRAHLLAEAELARALRHLHHGDSPEEVLQMLAYGLTN
                     KLTHDPTILLRNGEGLDDEDRDLMERLLNRFYRRHDR"
     gene            10591..11664
                     /gene="prfA"
                     /locus_tag="ACZPB6_02810"
     CDS             10591..11664
                     /gene="prfA"
                     /locus_tag="ACZPB6_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR1183443.1"
                     /translation="MNDNMRSKLRQLARRLEEIDGMLADPACASDMSKFRALTKERAD
                     IEPVVKKHLELESVDMDISTAQGMLEDPEMKAFAQEEILEAQKKLEVIEHELQILFLP
                     KDPNDSRNIFLEIRAGTGGDESALFAGDLLRMYLRYAEDHRWQTEIVSKNDSELGGYK
                     EVIVRIIGEGAYSKLKFESGGHRVQRVPATESQGRVHTSACTVAVLPELDEIGEVNLN
                     PADLRIDVFRASGAGGQHVQKTDSAVRITHLPTGMVVECQDERSQTQNKARAMAVLAS
                     RLHAAQVAEQQAKEASERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLYRLEAIMN
                     GDLEEIITALTTQHQAEQLAALA"
     gene            11683..12513
                     /gene="prmC"
                     /locus_tag="ACZPB6_02815"
     CDS             11683..12513
                     /gene="prmC"
                     /locus_tag="ACZPB6_02815"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR1183444.1"
                     /translation="MNEVKLNTIRALIGEATPKIGRFEAMILLAHILGKPKEYLIAHD
                     DEELPDTTLMTFDVYVSMRLDGVPVAYLTGRQEFFGRYFTVDHNVLIPRPDTEVLIEQ
                     ALIVAPEKPEILDMGTGSGCIAVTLAKECPGARVTATDVSEEALAIARGNAEALGAEV
                     EFRKGSWWEAVPEGARYDLIVSNPPYIRSDDEHLPALRHEPQGALTDSGNGLSCLEEI
                     ATHAMSHLRHGGWLLVEHGWDQAPDVAALFRSVGLAAVRTVRDYGGNDRVTMGRKADD
                     "
     gene            complement(12970..>13761)
                     /locus_tag="ACZPB6_02820"
     CDS             complement(12970..>13761)
                     /locus_tag="ACZPB6_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183445.1"
                     /translation="EELRRMMKEWIESSGADVLSSQTRRVAKRFALVAAAGELAAAFG
                     VLPWDRGEASRYAAACFRSFLADFETSEDRALRLCEIPFQLIDSYPENFSYHMPDGGR
                     TEAKYTTPFYGDVVSDMPVDGGEGRGAPALVLMTGEGWKKACGRNAAEIGRAMMTMGY
                     LLSNNGRKFQHNGSADTFLGIPIHGRYIFIREGSYSAKVADFLAETYFSKGAVCEMRK
                     KAMRRIVQSTVAQPCNLINRSKNGTLPRKACATGLQTDKPDKPKK"
BASE COUNT         2790 a         4613 c         3628 g         2730 t
ORIGIN      
        1 ctcttcgccg gaattgaaag agaagaatta accttcgtct ttcggctttt ccttcaaagc
       61 gcgaacttcg taagtttttt gagtgcctct gaaagtcacc cttgaaactg ccgatgttca
      121 cacttatcgg ttcgttgatc aaatttttaa agaactcgct gtttaaacag cgtaggagtt
      181 tgattctaca gaaccaattt ccgtttgtca agtcattttc ttgaccgact tttcatccga
      241 cctccgaaga agtcaggtgg cgtattatgc acaagagttt ttgagttgtc aagactcgtc
      301 gaaaaaagtt tttgaaggac cttcatgttc cgctatacag tcacaccgga tcctcacgcc
      361 cacctgctgc gcgtctcgct cgagctgacg ccgccgcaag acgccgttac acttcacatg
      421 ccttcctgga caagcggcag ctatctcatt cgcgactacg caggccgcat ccgcggcccc
      481 agctccaaca caggcacgct cgtccagacg gacaagtgcc actggaccgt ctcgggtctg
      541 acacccggcg ccacccttcg catcgaatgg gaagccttca cttattctgt aggcattcac
      601 gacgcttggc tcgacgacat ccgcggcttc tacaatccgg cagccgtgct tctcatcccc
      661 gaaggcatcg acgctccctg ccttctcgac ttttccgact gccgctttgt tcctcactgc
      721 agtcttgaac ccatcggcac gcacagtttt gcagcctcct gcacccagga gcttctcgac
      781 gcccccgtca ccttcacgtc tgcaaagggc gaacactgca ttctcaacct ctccgtctgc
      841 ggcattcccc acacgcttgt cttcaccggc cccggctcct ccactctcaa caaggacctc
      901 atcctgcagg atgcacaagc cattctgaaa accgtcctcg acttctgggg cgccgccccc
      961 ttccgtcagt atctgtttca catccaaatc ggtccccgtc tctatggcgg tctggaacac
     1021 gaagcctcct gcgtgcttca gacagacaca ctggcccttc ccggcgccag cgaatcctcc
     1081 cgtccgaaag actatgagga cttcctcact gtcttcgctc acgaatactt ccatgcctgg
     1141 ctggtgaagc atcttcgtcc cgcatccttc ctgccctaca agctcgagcg cgaatgccac
     1201 acccacgacc tctgggtctt cgaaggcttc accacctatt acgagaacgt tcttcctttc
     1261 agagcgggcg tactggacga agacaagctc ctctccctga cctccgcacg cttcaaccgt
     1321 gttcgcgaac gcgagggctt ccacgtcgag tccctgtcag acgccagctt caacgcatgg
     1381 acgcacctct acaagcagac gcccgacagt gcctactgtc aggcctccta ttacggaaag
     1441 ggagccgtcc tcgccttcat gcttgacgca gccctccgca aggcgggatt ctcccttgat
     1501 gaagtgctga agacctggtt tcacgaggca cttgccgacc cgcaggcaag atcccttcct
     1561 gacggcggct tctttgaacg catccccgtc gcatccatcg ccgaacgctt caaaaaactc
     1621 gtcacgtcct cggaccgcac cctttgggag aacgaatggc gctgcgccct gtccaccctc
     1681 gggatgtcgg aggaagcgga caagaacacg cctgcgtcca gaatgcacct cggccttcag
     1741 cttgacgggc ttcgcgtccg ctacgccgaa agtcgcggtt ccgcctatgc ggccggtctc
     1801 ttcgcaggcg atgaaatcat tgccgtgaac ggccttcgag ccgacgccgc caccatcgag
     1861 cgcatgattg ctcaggccaa gggctcaacc gtgcacattc actacttccg cgagcacctg
     1921 ctgcgaagcg ccgaaatcga gattcccgtc aatcctcttc cccaacccgg caccttgaaa
     1981 atgaaaaacc cgaccgaatt cggccgggca tggatcagat cgtccaatcg acaggcctga
     2041 ggccatgctc cgccagccac gtgttggcgg cgccgaagtg accacacccg atgaaaggca
     2101 cgggcggccg caacgctgaa agcggagacg gatgattggc acagaggacc agatgcttct
     2161 cctcttcgat gagagcgcgc ttgccctgag cgaaattgcc ccaaagcatg aaaacggcat
     2221 gcggcgccgt ctccgacacg gcccgaatga tcgagtcggt tagcgtctcc cagcccaggg
     2281 agcggtgact agcagcctct cctcccctca ccgtcagaat ggcattcaga aggagcaccc
     2341 cctgatgcgc ccaggacaga agcgatccct gctgagtcac ggacacgccc agatccctct
     2401 gcaactcctt cttgatgttg cgaagcgacg gcggcacctt gatgcctgcg ggcacggaaa
     2461 aggaaagccc ttcggcctgt cccggaccgt gataggggtc ctgccccaaa atcacgaccc
     2521 tgaccccatt cgacggcgtc tcgtccatgg cacggaatac acgaggcgga taaacctgat
     2581 accctgccgc cacatcctcg gccacgcgct ccacggctcg ccttcccttg tccgtcgcca
     2641 tgaaggcctc aacaagttca tgccacagag gatccgcagg caacgtcatg tccggcatga
     2701 cgccttcaga aaacaggctc gccgtcattg ctttgccgaa ctcaggtagc ggttttcaag
     2761 catgtcggtg cgcatgcggt agagattgat cctgcaggca agcgccgcat tgttctcctg
     2821 cgtactgctt cccttggtcg tattcttcac gaagtcgcat tcgcgcttga tgaagttgtc
     2881 gaaggcctga ttggccctca tgagcttgtc ccaacgcgtc ctgtttcgat tgggcttgtc
     2941 ccaggctttc gcgacaaccg tcacgcgttc gaccgcatcc ttgtactccg cctgcacaag
     3001 cttgctctcc tgctccaagc aggcgccgat cttcacggcg tcatcacccg ccttctggta
     3061 gcacatgttg atctccacca tgggagagac agcgtgcgcc gcaaaggatg cagcggccag
     3121 cacgaggccg caaagcaaaa ttctcttcag atccatttct ctgacgtcga tccttccgga
     3181 ttcatccgcg cggatcgatc aggatccgcg cctttaacaa attacagtct gcctgaggcc
     3241 acgatccgct cgcacacctt tcttgcgctc ggatagacgc gggtgaatcc cgcctctcgg
     3301 gcccagtcgg caatgcttac gccctcgttg tatccggtct caaccagagc caccgaaacc
     3361 cccgccgcac ggcccgccag cgcatcgttc cggctgtcgc ccaccatgac ggtttccgac
     3421 gcacagacgt tcatctctgt catggctcga atcagcatgt cgggagcagg cttgttgttc
     3481 gggcagtcat cacccgccac caccgtgtca aagagactct ccagtccacg ggactggagg
     3541 aattcgaccg tgaggcttct ctgcttgttc gtcacaagcg ccaccctgat gcccttgtcc
     3601 ttgagctgct cgacgccctc gatcaccccg ggatagaaag ccatgtgcct gccgcccgac
     3661 tcactccatg ccttgagaag acgatcgaac aaatcgcgtc cctgctcgga ttcgacggca
     3721 atgcccagag cggcacagag acgctgaaca agcacgagga tccccctgcc gatcatggcg
     3781 cccaccgcct cctgggacgg cgccttgagc cccatgtccg acaatacggt gtgaacgccg
     3841 tggtacaact caggaagaga gtccaccaga gtgccgtcca gatcgaacag aacagccttg
     3901 accagtcgca ttctttactc cagcgccgca acggcagcct tcattctgga gatcacgtcg
     3961 gcataacccg ccgcattgcc cgccccgaaa acagcggagc cggcaacaaa ggtgtcggca
     4021 cccgcgaggg ccacttcggc aatattggca ggcttgatgc cgccatctac ttcaagcgcg
     4081 atgcgacggc ccgtttcagc ctcgtagcga tcgagctttt cacgcacggc acggatcttg
     4141 tcgagcgtcg cgccgatgaa gctctgcccg ccaaagccgg gattcacgct catgagcaac
     4201 accacgtcaa tgcgatcgaa ctcatagtcg agataagtga gcggagttgc aggattaaag
     4261 acgagaccgg acttcatgcc gtggtcacgg atgagctgca gggtgcggtc gacatgcctc
     4321 gacgcctcgc aatggaacgt gatgatgtcg gcgcctgcct tcgcgaaggc gggcacgagc
     4381 tcgtcaacgg gctcgaccat cagatgcacg tccacgggca ccttggagta cggacgaatt
     4441 gccttgagca cgagcgggcc aacggtcaga ttcggaacgt agtggttgtc catcacgtcg
     4501 aagtggatcc agtcggcgcc gccttcgaca acgtcgctca cctcctgccc gagacgggcg
     4561 aagtcggcgg aaagaatgga aggggcaatt cggcaaagtc gcatgattga aagttccttc
     4621 tccgcatgga atgcgggtga cgaatgttga cagggttcat tgtatagcga gagccgtcgt
     4681 cggactcagg gcccgtcccg cacctttcat ttttttcaac cgtccctatc ttttcgccta
     4741 gaatcaacgc acgcgactcg atccaccttc tctttccaga cgactcaaca tgctctccag
     4801 actcatccgc cttgcggcgc ctgccgctgc actgctcctt gccgcctgca cgacgcccga
     4861 gccgccggtc aagcccgtac cgcccgctca tcccgaggtc gcctttgagg agagttcgtt
     4921 tgaccgcctg cctcagattc aggaccacac ctgggagcct gcactcgcag ccttccagcg
     4981 ttcctgcgag gtcatgcagg caggccccgt ctgggaggac gtctgtcgaa gtgcgctcgc
     5041 gaccgacccc atcggcgccc agcgcttctt ccttgcgaac ttcacgccct ggaaggtctt
     5101 cgtcgccaat gatgacgcga cgctcgttca ggatacgggc ctgatgacag gctactacga
     5161 gcccgagctg ctcggcagcc gcacgcgaag cgctcccttc acccatccga tctacggcgt
     5221 gccggacgat ctgctcgtca tcgatcttgc cgaactctac ccgcagctga aggagcttcg
     5281 ccttcgcgga aagcttgagg gccgacgcgt cgtgccctat gacgaccgcg caaccattca
     5341 gaaacgcacg gatcttgcgc cccgcgccat cgcctgggtg aacgatcccg tcgacgcctt
     5401 ctttctccac attcagggct cgggccgcat caggctcccc gacggcacct tcatgcgcgt
     5461 gggctttgcc gaccagaacg gccgcaagta ccactcgatc ggcacctggc tcatccgcga
     5521 aggccatctg aaaagccacg agctttccat gcagcgcatc cgtcgctggg cccgtgaaaa
     5581 tccttctcgt gttgacgagg ccctcgccca gaatcccagc tacgtcttct ttgaagaacg
     5641 caaaggctcc cccgatctcg gcccgctcgg tgctcagggc gtgcccctta cgcctgaagc
     5701 gagcgttgcc gtcgatcttc gctactggcg cctcggcact cccttcatcg tcgacgcctc
     5761 tcaggcctcc cccgctctga agatcgtgcg ccccgtcatc gcacaggata cgggcggcgc
     5821 cattcgcggc atcatccgct tcgactactt ctggggattc ggcgacaagg ccggcgaggc
     5881 tgctggccgc cagaagagca acacattctc ctgggtgctc gtacccaacg gcatgaagcc
     5941 cgaggacatc atggtccgcc gctgacgccg aagggcaggc cgcaaagaca aaaagggagg
     6001 cgttcgaaac gtctcccttt ttgtcgtcaa aaacttcgag gcttattcca ccgtcgagac
     6061 gagcttctcg aactgagagg ccgccatggc gcccgtcacc ctgtcgccgc tcttgaagaa
     6121 gaaggtcggc gcgcctgtca cgttgaggcg attggccagc acgaggttgc gctgcagcat
     6181 gctcgtgtcg caacccgcca gaacggagtt cggacgcaca ttgttgccca tccattcatg
     6241 ccaggccttg gcgcgatcct tggcgcaaac gatctcgcgg gcattcgtct ctccgcgcag
     6301 catcggcgtg atgaaggtgt agaccgtcac gttgcccacc tgagcataga ccgactccat
     6361 ggcgcagcag taggtgcagt tggcgtcgga gaagacgaca acctcgcgct cacccttgcc
     6421 gaagacctgt ttgacagcat cctgaaacgg ccattccttc cagcgctcgc gagaccgctc
     6481 gcgaagacgt tcattcgtaa ggttcgtctg cttgaccgta tcgatcaggc tgccgtagag
     6541 cacgtagcgc gcctgatcat cgacatagaa caccctgtcc tgaaccacga cctcccacat
     6601 gccgggcacg ggcgacttct ggacagcgtc cgccttgagg ccggtcgtct gcatgagcgc
     6661 atcctcgaca ctctggggcg ttgctgcaaa gacggtaacg gcaaggcccg cacatagggc
     6721 tacaaacaaa ctcttcttaa gcatgatgtt cttcaagatc tcttcgttga tcaattgtcg
     6781 gagcgggctg aaccgccttc tcggagtcta cccgaacggc acgcgcctca acactttctt
     6841 tttgacatcc ttccgggtgt gaatacccgg gtttcctcgt attcgcgtca agacgcgaag
     6901 aaaaggacgg ttcctgccgc tgacttgttg ccaagttcac ttgacgggcg aactgagcct
     6961 gtccggctct tcttcagcac gttgagtgca cctgcgacgt ccgcgttttc agtgcagccg
     7021 caaagttggc aaccgaaatg cgcctgcgca ggtcgatttg ttgaactcgt gtatccgcac
     7081 ctgggacgag tctggctcgt gtacttcgga tcgacgagga tgagccgtcc gtcggccttg
     7141 aacaaggccc attggatggc catcctcata ccatagggcg caacgcgtgc caaagaacgg
     7201 ttcaagccgc tcttctgctt taggttcctg cccggatcct taacagtacc ttcagcagac
     7261 ttggtcatgt tcttcagatt cataaccttc ctttctggaa accgctcagg accggattat
     7321 ccgcgcagtc cactgcacca cacgcgcttc gcctgccctc ccgcatcgag caagtacgcc
     7381 gtccatacca aatttacggt acaatccacc tcttttacga ccgtactatt gcctttttcg
     7441 aaaggcgggc atataatggt catctaattt gcgcggctgg gtagctcagt tggtagagca
     7501 gcggattgaa aatccgcgtg tcggcggttc gattccgtcc ccagccacca caaccttcgt
     7561 ggggcgcgga ttgacaattt gtcctgatgg ctgggtagct cagttggtag agcagcggat
     7621 tgaaaatccg cgtgtcggcg gttcgattcc gtccccagcc accatactgc aagaagcaga
     7681 ctgtgaagac ggcctgcttt ttttgtatcc acagtttccc cttccctccg cccggacggc
     7741 aaaaacggac gcgcctcccg gccacgtccg ttctctcaag gattcctccc cgattgtcag
     7801 cccgtcatcg gtcgcaatat cgctcccagc cctttgcgcg aagctgacag gcaggacacg
     7861 ttccgcagcc gtagccccac tcatgaacgt gctcatgatc gcccagatag caggtgtgcg
     7921 tgtcaagacg gatcaagtcg accagttccc tgccaccaag cgcttccgcc agcatccagg
     7981 tctgagcctt gtcgagccac atgaggggtg tctcaatgac catcggcgca tcgaggccaa
     8041 gcgaaagcga gacctgcagg ctcttcaatg tgttgtcgcg gcagtcggca tatccggaat
     8101 agtcggtttc gcacatgccg cccacgagcg tttcaatgcc tcttctgtaa gcgagcgcgg
     8161 ccgcaaacgt gaagaacaga aggttccttg cgggcacaaa ggtgttgggg atgttgttct
     8221 ccatgaagcg aagctcctgt tcgcgcgtca gcgcgcagtc gctgatctgg ccgagaagcc
     8281 ccatgtccag catgtggtcc tcgccgagcc tctgcaccca ttcgggcctg aaggcgcgga
     8341 gcttctcgag cacggtcttc ctgcactcga gctcgaccgc atggcgctga ccgtagtcaa
     8401 accccagagt ctccaccctc tcgaagcgcg agagagccca tgcaaggcac gtcgtggagt
     8461 cctggccacc ggaaaagaga accatggccg cgcccctgcg ggcgggctgc attgcattcg
     8521 tttcactcat gattgttgtg cgtttttctt caagctgtcc ttttcataag gcaggagtgc
     8581 ggccacgcgg cttcgcaccg caaggcattc acggacgtgc tgctctccga gccacttgac
     8641 gaggccgtag gagagagccg ccgcagcacc ctgtccgaca atcggcaccc agcgggcggt
     8701 ctttcccgcg caccatcggc tgccgagctg cttcgcaatt gtaagcgctg cgacgcccgt
     8761 cacggatttg cccgccatca cggcgccgac gcccgaaaga gcctcgtaaa ccgccacgcg
     8821 ctcctccatg ccgagcctct cgatctgatc gggactcagg cagaaggcac ggttgacggc
     8881 gttcatcatg ctcaccagca cgccgacatc caccgccaca tcgagccccg gcagcggcac
     8941 aagcacggcg cctgcgctca aaagcgcgcg ccgcttcact tcctcgcggg cccgcctggc
     9001 cgccagcgcc agttcacccg ccgcgggatc cgcccactca tcacgcatga cgctcctcct
     9061 gcaagtgctt ccttcttaag ctatacacaa cgttgagccc tatataattc gcgcattatt
     9121 aaacattgtt gcaacttttt cgttgcgctc ctctttccca tccccgacgc gatgcatctt
     9181 cttgctatag gtctcaacca tactaccgct ccggtttccg tccgagagcg cgtagccttt
     9241 ggtcccgaag aaatcgccga aaccatcggc cacatgcgcg agcggttctc cagcacgcag
     9301 atgggcggca ttcacgaagc cgccattctg tccacctgca accgaaccga aatctactgc
     9361 gccgccgaag acacggacgc cgccagagac tccgtgctcg gcttcatctg cgaacgcaag
     9421 aacgtcagcc gctccgaact cgagcctcac atctatacgt tcacgcagga agaggccgcc
     9481 aagcacacct tccgagtggc ctccggcctc gactccatgg tgctcggcga aacacagatc
     9541 gtcggtcaga tgaagaaggc cgagaagatg gcccgtgacg cacacggcct gggcacgatg
     9601 ctcaaccatc ttttccagtc caccttcacg gttgccaagg aagtccgcac cgccaccgcc
     9661 atcggcgcca actccgtgag tctcgcggcc gccgctgtgc gcctggcgct tcgtctcttc
     9721 ggcagcctca aggacgagcg cgtcctcttc gtgggtgcgg gcgaaatgat cgagctctgc
     9781 gcagcccact ttgccgcgca gcagcccaag tccatcacga tcgcgaaccg cacgctcgag
     9841 cgcggtcagg ttctcgcacg caccgtgcac gccgacgcca tccgtcttgc cgacctgccc
     9901 gagaagattg ccggctacga catcatcgtc tcctgcacgg cctccgccct gccgatcatc
     9961 ggtctgggca tgatcgagcg cgccatcaat gaacgcaagc accgtccgat gttcatcgtc
    10021 gacctggccg ttccccgtga cgtcgagccc gagatcgatc gtctcgacga cgtgtacgtc
    10081 tacaccgtcg acgacctcgg caaggtggtc aaccagggca tggcaagccg acaggccgcc
    10141 atcacccagg ccgaaaccat catcagcctg cgcatgagcg acttcgccga atggctccgc
    10201 cagcgcgaga ccgtcccctt cattcagtcg cttcgcgacc gagcacacct tcttgccgaa
    10261 gccgagctcg cgcgcgccct gcgccacctc catcacggcg acagccccga ggaggtcctg
    10321 cagatgctcg cctacggcct caccaacaag ctcacgcacg atccgacgat cctgctgcgc
    10381 aacggcgagg gcctcgacga cgaggaccgc gatctcatgg agcgcctgct caaccgcttc
    10441 taccgccgtc acgaccgctg aggcggacaa ccctgacaca ccaagaggaa cctgcggatt
    10501 cctcttattt ttttgatatt ctttaggcct gaattctccg gctgcacgac tgcggcccac
    10561 acgccttcat cgacagacaa cgccatcatg atcaacgaca acatgcgttc caaactccgc
    10621 cagcttgccc gccgtctcga ggaaatcgac ggcatgctcg ccgaccctgc ctgcgccagc
    10681 gacatgtcga agtttcgcgc actcacgaag gagcgtgccg acattgagcc cgtcgtgaag
    10741 aagcatctcg agctcgagtc cgtcgacatg gacatcagca ctgctcaggg aatgcttgag
    10801 gaccccgaaa tgaaggcgtt cgctcaggag gagattctgg aggcccagaa gaaactcgaa
    10861 gtcatcgagc acgagcttca gatcctgttt ctgcccaagg accccaacga cagccgcaac
    10921 atcttccttg aaatccgtgc gggcacaggc ggcgacgagt ccgccctctt tgcgggcgac
    10981 cttctgcgca tgtacctgcg ctatgccgaa gaccaccgct ggcagacgga gatcgtctcc
    11041 aagaacgaca gcgagctcgg cggctacaag gaggtcattg tccgcatcat cggcgagggc
    11101 gcctattcca agctcaagtt cgagtccggc ggtcaccgcg tgcagcgcgt gcctgcaacc
    11161 gaaagtcagg ggcgcgtcca cacgtccgca tgcaccgtcg ccgttctgcc cgagcttgat
    11221 gaaatcggcg aagtgaacct caacccagcc gaccttcgca tcgacgtctt ccgtgcctcg
    11281 ggcgccggcg gccagcacgt tcagaagacg gactcagccg tccgaatcac ccacctgccc
    11341 acgggcatgg tcgtcgagtg tcaggacgag cgaagccaga cccagaacaa ggcgcgcgcc
    11401 atggccgtcc ttgcctcccg cctccatgcc gcccaggtgg ccgagcagca ggccaaggag
    11461 gcgagcgagc gcaagagcct gatcggctcg ggggaccgct ccgaaagaat ccgcacgtac
    11521 aattaccctc agggccgcgt gaccgaccac cgcatcaacc tcacgctcta tcgccttgag
    11581 gctatcatga acggtgatct cgaggagatc atcacggctc tgaccacaca gcaccaggcg
    11641 gagcagctcg ccgcgctggc ctaacaaagt acgtatcgcg tcatgaacga agtcaagctc
    11701 aacaccatcc gcgccctgat cggcgaagcg acccccaaga tcgggcgctt cgaggcgatg
    11761 atccttctcg cccacattct gggcaagccc aaggaatatc tgatcgccca cgatgacgaa
    11821 gagcttcccg acaccacgct catgacgttc gacgtctacg tttccatgcg ccttgacggc
    11881 gtgcccgtcg cctaccttac cggccgtcag gagttcttcg gccgttactt cacggtcgac
    11941 cacaacgttc tcattccgcg tcccgacacg gaggtcctca ttgagcaggc cctgatcgtc
    12001 gcccccgaaa agcccgagat cctcgacatg ggcacgggca gcggctgcat tgccgtgacg
    12061 ctcgccaagg agtgccccgg ggcgcgcgtg accgccacgg acgtctccga agaggctctc
    12121 gccattgccc gcggcaacgc cgaggcgctg ggcgccgaag tcgaattccg caaaggctcc
    12181 tggtgggagg ccgtccccga aggcgcacgc tacgatctca tcgtgagcaa tcccccctac
    12241 atccgcagcg acgacgagca tctgcccgcc cttcgtcatg aacctcaggg agccctgacc
    12301 gacagtggaa acggcctctc ctgcctcgag gaaatcgcaa cccatgcgat gagccacctc
    12361 cgccacggcg gctggcttct cgtcgagcac ggctgggacc aggcccccga cgtcgccgca
    12421 ctcttccgct ccgtcggtct agcagcggtc cgaaccgtcc gcgactacgg cggaaacgac
    12481 agggtgacga tgggacgaaa ggccgacgac tgagtcaggt cgaccgcgat tcaaggctcc
    12541 gcacaaacag gcggggcctt tcttgtgtcg gcccttgggc aaaagaaatc ccgcaccgcc
    12601 tgagacggta cgggatccga atttggtgcc ttatctgcat ataagatcgt tgcaccgcaa
    12661 cgattttaag agaaacaggc tattttggta cggtatttgg tacggtacgg cgcgcgtcac
    12721 cctccaaaat tgagcaaaaa ctaggattaa cccttagagg cgcgtcaagg tcttggagag
    12781 gcggcattgc caagagaagg caaccacgat aggccaccgt ctcctacagg caccccttca
    12841 ttgtgagttg ctttgcaccc ggcacccgca tcatgctccg gccccaccct cttgccgata
    12901 cataaaaaat gcctctcagc tatcaagccg agaggcgcca ctttttaccc cttacaccct
    12961 aggtctttgt cattttttcg gtttatccgg tttatcggtt tgaaggcctg tggcacaggc
    13021 ctttcgagga agcgtgccat ttttcgaccg gtttatcagg ttacaaggct gtgccacagt
    13081 cgattgcacg attctcctca tggctttttt gcgcatttca caaaccgcac cctttgaaaa
    13141 ataggtttcg gctaggaaat ccgccacctt cgccgagtat gaaccctcac gaataaaaat
    13201 gtaccttccg tggataggaa taccgagaaa ggtatcagca ctgccgttat gctggaattt
    13261 tcggccattg ttggaaagca aatatcccat ggtcatcatt gcccgcccga tttccgcagc
    13321 attccggccg caggcctttt tccatccttc gcccgtcatg agcaccagcg cgggagcccc
    13381 gcgcccctcc ccgccatcta ccggcatatc tgagaccaca tccccataga atggcgttgt
    13441 gtatttggcc tcagtcctgc cgccgtccgg catgtgatag gagaagttct ccggatagga
    13501 gtcaatgagc tggaagggta tttcacaaag acgcaaggcc cgatcctcgc tcgtttcaaa
    13561 gtccgcaagg aatgaccgga agcacgcggc ggcataccgt gacgcctcac cccgatccca
    13621 tggaaggacg ccgaaggctg cagccagctc cccggctgcc gccacaagcg cgaagcgttt
    13681 tgctactcgc cgggtttgag aggaaaggac atccgcaccg ctggactcaa tccattcttt
    13741 catcattcga cgcaactcct c
//
LOCUS       DBMDTI010000029        13663 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1383_length_13663_cov_2.798923, whole genome shotgun sequence.
ACCESSION   DBMDTI010000029 DBMDTI010000000
VERSION     DBMDTI010000029.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 13663)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13663)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13663
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1383_length_13663_cov_2.798923"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(166..573)
                     /locus_tag="ACZPB6_02825"
     CDS             complement(166..573)
                     /locus_tag="ACZPB6_02825"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR1183446.1"
                     /translation="MGIREPAIYGSTTYKDLCGILETWCADAGASCELFQSNHEGAIV
                     DRIQQAWFDGVDGIVMNPAAYTHTSVAILDALKATAIPTVEVHISDVSTREAFRHHSY
                     VSLAAKHTIIGHGIDGYREGIEWLARELRAGRL"
     gene            complement(455..2947)
                     /gene="aroC"
                     /locus_tag="ACZPB6_02830"
     CDS             complement(455..2947)
                     /gene="aroC"
                     /locus_tag="ACZPB6_02830"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR1183447.1"
                     /translation="MPYFGRNIRTAVFGQSHAAAIGVTIDGLPAGEAVDAVELEAFMA
                     RRAPEQNRMTTQRREADRPEILCGIVDGRTCGAPLTSIIRNGDTRSKDYAAIADLPRP
                     SHADWPAHVRYGGWQDVRGGGAFSARLTAPLCIAGGIAMQILARRGIRIAAHVRSVGA
                     AEDRPYDPMGESSETIEESLARSPVALSNEAAAAMAAEIEAARQEADSIGGTVECMIS
                     GLPVGLGGPLFEGLDGRIAQAVFAVPAVKGVEFGEGFGAALLRGSENNDPYGMREGAV
                     TPLTNRAGGIASGMTTGMPVVFRVALKPTPSIGQSQHTVSLSGGCDADLRITGRHDPC
                     VVVRAVPQQSSHLTPCSTPPPDYPTPPCAPPMKEELDMKQRFDLIGASLGHSLSPEIH
                     AKLGGYPYDLIELTEDEVGPFLTSGDFQGLNVTIPYKKTVMKHCTELTPRAAAIGSVN
                     TIVRLPDGGLLGDNTDYAGFAWMVKRAGLSPKGRNALVLGSGGASLTGCAVLRDLGAA
                     SVTTISRSGPDNYGNLDRHADAQLIVNTTPVGMHPTCDASPLETLEAFPALESVLDII
                     YNPACTELMLLARSRGLPTENGLSMLVMQAQAAAERFLGHALPDAAAEDILKDMTLQF
                     SNLVLLGMPGRGKTKVARRLAELLHRPFVDVDELIVRKAGRSIPDIFARDGEAHFRDL
                     ESKVIEGLSAGHCLVVATGGGSVLRERNRRLLQRNGLVLWLHRPLEELPSAGRPVSLA
                     RGVEAIFVEREPIFRALAHRIITSRRLRTQFGRSSEKSRETSDHQRPQHQFSGHSRTG
                     HLRQHDLQGPVRNTRDLVRRCRRLMRTFPVQP"
     gene            complement(2950..4206)
                     /gene="aroA"
                     /locus_tag="ACZPB6_02835"
     CDS             complement(2950..4206)
                     /gene="aroA"
                     /locus_tag="ACZPB6_02835"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR1183448.1"
                     /translation="MIATIQKGFLSGKIPAISSKSYAHRLLIAAALADRPTTVRCATV
                     SEDILTASRVLKAMGASVERTDDGFIVEPVSTQPAYCGESGTIERFILPVACALGLDA
                     SITGHGRLPERPLSPLYEELRDHGIELSPQGVFPLQTKGRLPAGDYAIAADVSSQFIG
                     GLLFALSVVPGTSTLTLTGRIESAPYIDMTLDVLALFGARITRSEDGRRFTVEGLERL
                     RSPGAAEVEGDWSNSAFWLCAGALGRGVTLTGLRADSRQGDRAVVKLLEAFGAEVSMT
                     EDAVTVRPGALRGMTVNAPQIPDLVPVLSVVAAFAEGETRFINAGRLRIKESDRLATT
                     RQLLEDLGADVEKIPDGLVVRGRTALRGGACDGTRDHRIVMSAAVAGARTCTPVVIRG
                     AQAVRKSYPDFFDDFARLGGLVFQED"
     gene            complement(4209..4887)
                     /locus_tag="ACZPB6_02840"
                     /pseudo
     CDS             complement(4209..4887)
                     /locus_tag="ACZPB6_02840"
                     /inference="COORDINATES: protein motif:HMM:NF049783.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
     gene            4847..5329
                     /locus_tag="ACZPB6_02845"
     CDS             4847..5329
                     /locus_tag="ACZPB6_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183449.1"
                     /translation="MEEEPYLREVFVGITRAKTRLRIHHGPGAFLPHAQLAGLAFVEC
                     SDETRLWPPAEVLQMSLGHDGLFLDYFISRQRLIEKLHSGQKLIPRDFELFCRTEGGG
                     EASVARFSKKAREDIASILAGGYVMSEASVRVMVYWRKKDSDADTLILLPDLVFRRRE
                     "
     gene            5681..5848
                     /locus_tag="ACZPB6_02850"
     CDS             5681..5848
                     /locus_tag="ACZPB6_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183450.1"
                     /translation="MMPFPWFYDLEYTPWLFGVPRFIYCWLAYGLLVIGTIALWWRSC
                     MKRPEYQEYED"
     gene            5853..7346
                     /locus_tag="ACZPB6_02855"
     CDS             5853..7346
                     /locus_tag="ACZPB6_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR1183451.1"
                     /translation="MPYQVETSTVPFLIVTISYLAVLGFIGWWGNRQNRNLQDFFTMS
                     GSAGAVLSGLAYFSTQYSMSTFMGVPGSIYHVGYAGMSVSVPGIAFSMVIPALLVGRR
                     LIKLGHRYKMLTMADYLADRYESTAIRGLLAILMLIFLVPMMGAQTIGAGVIFTTYTG
                     APQWVGIVVMGVTVILYCMAGGIRSVMMTDVIQGVLMVVTAVTTFFVSLKLGGGLETI
                     NAKLASVDPNYLKFPGHNNSYPWQNYASMIVMWSFFCIGQPHLFTKFFTMKNYGTMFK
                     AVLLGTLGMWFSASFIEWCGVTGAASFPGIPGKESDFIVPLILQEGLPSIVSALMVAG
                     IFSAGMSTISSLLLTSTSAVSRDLYQRILKRDATDEDTLKMSRVVTVVLGIIAIGIGI
                     LKPGSIFTIILFAFGGLGIWAAPIICGMYWKRTTTTGVFASVIIGEAFYFLLLTEFKS
                     LSMGFNPLIVAWLVTMMILIGVSLFTKPVSAETIRRHFDDLDKPVKA"
     gene            7440..8219
                     /locus_tag="ACZPB6_02860"
     CDS             7440..8219
                     /locus_tag="ACZPB6_02860"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR1183452.1"
                     /translation="MQLAHLNWQEAEKVLADPETLVVIPVGSTEQHGSVGPLGTDWLI
                     PQELCRRLDGRMEKLLIAPVIPFGVATHHINFPGTIDLGLETMIKVMGAVFESLYRHG
                     ARRFAVINGHGGNDPAIEKAALEMYRRGSQVTLVNWWGIAPKLNPDWPTGHGDAQEAA
                     AVMAFAPELVKSEWLVDNVVTPPSADFKQLHINTVLFEGAPVKVIREIRDTVNTGGLG
                     GLESKFATREWGVAMMDGVTDWMVRYLETFRRIDLPKKHWD"
     gene            8224..9897
                     /locus_tag="ACZPB6_02865"
     CDS             8224..9897
                     /locus_tag="ACZPB6_02865"
                     /inference="COORDINATES: protein motif:HMM:NF016289.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR1183453.1"
                     /translation="MAYEDILAAVDAVRLMDSVRAICTGERLSNEAEVRSFDVLEKLF
                     TDAGCRVTREALPGYVSTPEGAAFEAGGIAYEVLTHPMTPSADGLEADLVYVPVDRTG
                     SASAEEVSGRIVLTDGLAMEPVVRALEDRGAAGAVFITGEEIHNMIVSRVWGSPTPET
                     LHGYVGIPVASLSFGDGSRLRARLSEAGGTLPARLSTRVESRWTEIPVITAEIRGADE
                     DFVMVTGHVDSWGLGALDNASGNACAVELARILAPLASDMRRSVRFVLWSGHSHGRYA
                     GSTAWCDAHFEELERHCLLNVNSDCLGGRGATVMTESPAMASTRGLARTALREAAGVT
                     DWRGCRFSRSCDQSFWGAGVPSIFSQVSEQPPFEGAAADAFGKMFGSGRTGGFGAWWH
                     TRTDTPDKLDPENLARDVRVFASVLAHALFDERLPVDAAAEVLELRDELKAWQEKAGD
                     SLDLSEVLARLDLLAEALKAAARSDDPKRFEKRSRRVLSEIIPLAYVEGSVYSHDEAL
                     RAPPVPMLRLVDELATLSGHERNAALVSLRRVVNRLKAGAARALDVARA"
     gene            complement(10038..10856)
                     /locus_tag="ACZPB6_02870"
     CDS             complement(10038..10856)
                     /locus_tag="ACZPB6_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR1183454.1"
                     /translation="MTSELLNALAQTTLATGFTFLCTAAGAAAVFFMKPGNGRGRGAE
                     LAETLSLGFAAGIMIAASVWSLIIPAQEMAQAQGGTPWLAAGSGVAAGVLFLKLLDTA
                     LPHLHPGAARPEGPKTSMHRAMLLFIAITLHNVPEGGSLGLVAGSAALSGEPAELSSA
                     LALALGIGIQNIPEGAAVSLPLAGQGMSRMRAFLFGAFSGIVEPVFGLLVVLVLQTVM
                     PAMPFMLAFAAGAMLYVVVEELIPAAHLSKHSDAGTLAVITGFILMMTLDTALG"
     gene            11071..11568
                     /locus_tag="ACZPB6_02875"
     CDS             11071..11568
                     /locus_tag="ACZPB6_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1183455.1"
                     /translation="MTKRLMPTRENLRRVHRGAAMSEEDIAGVELMLYLIRAADVVRE
                     SVYSGLRERELSEGKFALLMTLFDADGPVPVVELAKRIGVSAPTTSVMVTRMLQAENP
                     LIAKVQSKADARCWLIELTDEGRALITAELPAHTARVADFAKCLSGDECEQMIDMLKK
                     LVADD"
     gene            11898..12962
                     /locus_tag="ACZPB6_02880"
     CDS             11898..12962
                     /locus_tag="ACZPB6_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR1183456.1"
                     /translation="MNSTQVFEKRKIAVAIGIDLAKDHCDVVAYNADNKICFTKANMT
                     YPKLMEWLANAQPAVVLMESCKGCHVRARDIADLGHDTRLVKGADVKALRNVCQKNDI
                     RDADYIARLLYVPGTKFVFVKNQRQQSLQFLQNEYKSFQELRIRMGNQIHAGLEEFGC
                     PAPKSQQFVQSRMMAHLEKNAELIPEEVMASFLRRREMWLNLFKQEEDAKKRMEEIAK
                     TDDDTKRMMTVPGVGAQTAVGMLVHIGGDIGRFKNSGQFAASIGLVPKQNITGGNTTL
                     NGITKRGPKRLRSNLVQCGQVILMHADKLKGNFGDWVRKMRDSGKKRGVIACAIAAKI
                     ARIIYRLMKDKVDYTPVPSR"
BASE COUNT         2572 a         4167 c         4284 g         2640 t
ORIGIN      
        1 gttgatgcac agttgttttc gactttattg atgtacgctg tggcttgact gtgcgacata
       61 aaaaagctcc tgtttcggga gctttattat tatgagattg tactctaaaa agaacagtct
      121 ttctacaaga tatcatcggg acggttcgcc cttcggcagc gttcctcaga gcctgcctgc
      181 cctcagttct cttgcaagcc attcgatgcc ctcccggtag ccgtcgatgc cgtggccgat
      241 gatcgtgtgc ttcgcagcga gcgacacgta ggagtggtgt ctgaaggcct cgcgggtgga
      301 gacgtcggag atgtgcacct ccaccgtcgg aattgcggtc gccttgaggg cgtcgagaat
      361 tgcgacgctc gtgtgcgtgt aggcggcggg gttcatcacg atgccgtcga cgccgtcgaa
      421 ccacgcctgc tggatgcggt cgacgatggc gccctcatgg ttggactgga aaagttcgca
      481 tgaggcgccg gcatcggcgc accaggtctc gagtattccg cacaggtcct tgtaggtcgt
      541 gctgccgtag atggccggtt cgcgaatgcc cagaaaattg atgttggggc cgttgatgat
      601 cagaagtttc acgacttttc tccgatgatc tgccgaactg cgtcctcaac cgtcggcttg
      661 tgatgatgcg gtgggcgagg gcgcggaaaa tcggttcccg ctcgacaaaa atggcctcga
      721 cgccgcgtgc gagcgagacg ggacgcccgg cggacggcag ctcctcaagg ggacggtgta
      781 gccataagac gaggccgttg cgctgcagga gccggcggtt gcgctcccga agcacggagc
      841 ctccgcccgt ggcgacgacg agacagtgtc cggcggagag cccctcgatc accttcgatt
      901 cgaggtcgcg gaaatgcgcc tccccgtccc gggcgaagat gtcgggaatc gatcgtccgg
      961 cctttcggac gatcagctca tccacgtcga cgaagggacg gtggaggagt tccgccaatc
     1021 gtcttgcgac cttcgtcttg cccctgccgg gcatgccgag gaggaccaga ttcgaaaact
     1081 gcagcgtcat gtccttcagg atgtcctccg cggccgcatc gggtaacgca tggccgagaa
     1141 agcgttcggc cgcggcctga gcctgcatca cgagcatgga gagcccgttt tcagtcggca
     1201 ggccgcggct tcgggcgagc agcatgagct cggtgcaggc ggggttgtag atgatgtcga
     1261 gcacgctctc gagcgcaggg aaggcctcca gcgtttcgag cggcgaggcg tcgcaggtcg
     1321 gatgcatgcc cacgggcgtc gtgttgacga tgagctgtgc gtccgcgtgc cggtcaagat
     1381 tcccgtagtt gtcgggcccc gagcgcgaga ttgtcgtgac cgaggccgcg ccgagatcgc
     1441 gcagcaccgc gcagcctgtg agggaggcgc cgcccgaacc gagcaccagg gcatttcggc
     1501 ccttgggcga gagcccagcg cgcttgacca tccaggcaaa gcccgcatag tcggtgttgt
     1561 cgccgagaag tccgccgtcg gggagcctca cgatcgtatt gacggagccg attgccgccg
     1621 ctcgcggcgt cagttccgtg cagtgcttca tgaccgtttt cttgtacggg atcgttacgt
     1681 tgaggccttg aaagtcccct gacgtcagga acgggccgac ctcgtcctcc gtgagctcga
     1741 tcaggtcgta aggatatcca ccgagttttg cgtgaatctc gggggagaga ctgtggccga
     1801 gactggcgcc gatcaggtca aatcgctgct tcatgtcgag ctcctccttc atcggggggg
     1861 cgcacggggg cgtcgggtag tctggcgggg gcgtcgagca gggcgtcaag tgcgacgatt
     1921 gctgtggcac ggctctgacg accacgcacg ggtcgtgacg gcccgtgatc ctgaggtcgg
     1981 cgtcgcatcc gccgctcagc gagaccgtgt gctggctttg tccgatcgag ggcgtgggct
     2041 tgagagcaac cctgaagacc acgggcatcc ccgtcgtcat gccgctcgca atgccgcccg
     2101 cacggttggt gagcggcgtc acggcccctt cgcgcatgcc gtagggatcg ttgttttcgg
     2161 atcccctgag caacgccgca ccgaatccct cgccgaattc gacgcccttg acggcgggca
     2221 cggcgaagac ggcctgggcg atgcgcccgt caagcccctc gaagagtggt ccgccgagtc
     2281 cgacgggcag gcccgagatc atgcactcga ccgttccgcc aatgctgtcg gcctcctggc
     2341 gcgcggcctc gatctcggcc gccatggcgg cggccgcttc gttggagagc gcgacgggcg
     2401 agcgcgcgag gctctcctcg atcgtttcgg acgactcgcc catggggtcg tagggtcggt
     2461 cttcggccgc gccgaccgag cgcacgtggg ccgcaatgcg gatgcctcgt cttgcaagaa
     2521 tctgcatggc gatgccgccc gcgatgcaca gcggtgccgt caggcgcgcc gaaaaggcgc
     2581 cgccgcccct cacatcctgc cagccgccgt acctgacgtg ggccggccag tcggcgtgcg
     2641 atgggcgggg caggtcggcg atggccgcat aatccttcga gcgcgtgtcg ccgttcctga
     2701 tgatcgacgt gagcggcgcc ccgcaggtgc gcccgtcgac gatgccgcag aggatctcgg
     2761 gcctgtcggc ttcccttcgc tgcgtcgtca tgcggttctg ctctggcgcc cttctggcca
     2821 tgaaggcctc gagctcgacc gcatcgacgg cttcgcccgc aggcagcccg tcgatcgtca
     2881 cgccgatggc ggccgcatgg ctctggccga agacggccgt ccggatgttc ctgccgaaat
     2941 agggcatgat cagtcctcct gaaagaccag tccgccgagg cgggcaaagt cgtcgaaaaa
     3001 gtcgggatac gatttgcgaa cggcctgtgc gcccctgatg acgacgggtg tgcaggtgcg
     3061 agcacccgcg acggctgcgg acatcacgat gcggtggtct ctcgtgccgt cgcatgcgcc
     3121 tccccgaagc gccgtgcgtc cgcgcacgac gagtccgtcg ggaatttttt cgacgtccgc
     3181 gccgaggtcc tccagaagct gccgggtcgt cgctagacgg tcgctctcct tgatgcgcag
     3241 gcgtcccgcg ttgatgaagc gggtctcgcc ctcggcgaag gccgccacga ccgagagaac
     3301 cgggacgagg tcggggatct ggggcgcgtt gactgtcatg ccgcgaagcg cgccggggcg
     3361 caccgtcacg gcgtcttccg tcatggaaac ctccgcgccg aaggcctcaa gaagcttcac
     3421 gaccgcccgg tccccctggc gggagtctgc acgaagcccc gtcagcgtga cgcccctgcc
     3481 cagagcgccc gcgcagagcc agaaggcgct gttcgaccag tcgccttcga cttcggcggc
     3541 gccggggctt ctcagccgct cgagcccctc gacggtgaac ctgcgcccgt cttcgcttcg
     3601 cgtgattctc gcgccgaaga gcgcgagtac gtcgagcgtc atgtcgatgt agggcgcgga
     3661 ctcgatccgc cccgtgagcg tcagcgtgct cgtgcccggc acgaccgaca gcgcgaagag
     3721 caggccgccg atgaactgcg acgaaacgtc ggccgcgatg gcgtagtcgc ccgccgggag
     3781 gcgtcctttg gtttggagcg gaaatacgcc ctgcggcgag agttcgatgc cgtggtcgcg
     3841 gagttcctcg tagagcgggc tgagaggccg ctcgggcagc cttccgtgcc cggtgatgct
     3901 cgcgtcgagc ccgagcgcgc aggcaacggg cagaatgaat cgctcgatcg tccctgattc
     3961 gccgcagtac gcgggctgcg tcgagacggg ctcgacgatg aatccgtcat ccgtgcgctc
     4021 gacgctggcg cccatggcct tgaggacccg ggaggcggta agaatgtctt cggaaacggt
     4081 tgcgcagcgc accgtcgtcg gacgatcggc cagcgcggcg gcgatcagca ggcggtgtgc
     4141 ataggacttg gatgagatgg cggggatttt gcccgaaagg aagcccttct ggatggtggc
     4201 gatcatgatc agggattgag tccgagttgg atgaggtcgc gaagcttttc aaagaaggtt
     4261 ctttccgtgc ggcaccgccc gaaggcctcg ggcaggataa gcgtgatggc gctccccgcc
     4321 gatttcttgt ccgatcgggc cgcctcgaga agcgcctctg catcgtatgg acacaggcca
     4381 gtgagcccga acttttcgag caggtgctcg aggacgggca gggcctgcgg gtcgcagagc
     4441 ccggcgcgca tgcagccgcg gaagatgacg gccatgccga tgccgaccgc gcgtccgtgg
     4501 gtgacgccgt agtccgtcac gggttcgacc gcgtgcccga cggtgtgtcc gaggttgagg
     4561 agcgcgcacg ccgccctcga actcgtcctc cgagacgatg tcgcgcttga tctcgacgca
     4621 ggtcgaaatg atgtccgcca gcacctcttc ggtgacgggg ccttcgagcg tcgggagaag
     4681 gccgtcggcc ctgagcgccg catacttgat gactttggcc cagccgcagg acatttccgc
     4741 ttcgggcaga gtctcgaggc agctcggggc gcagaccgcg agcacgggct gatgaaaggc
     4801 gcccatgagg tttttgccgc actcgagatt gacggctgtt cgaggcatgg aagaggagcc
     4861 gtatctgaga gaggtgtttg tcggcatcac gagggctaaa acacgcctgc ggattcatca
     4921 tggaccgggg gcgtttctgc cgcatgccca gcttgccggc cttgcgttcg tcgagtgttc
     4981 tgacgagact cgtctctggc cgcccgctga agtgctgcag atgtcgttgg gacacgacgg
     5041 cctgtttttg gactacttca tttctcgaca gaggctgatc gagaagcttc acagcggtca
     5101 aaaactgatt ccgcgcgatt tcgagctctt ttgccggact gaaggcggcg gcgaggcatc
     5161 ggtggcgcgg ttttcgaaaa aagcgcgcga ggacatcgcc tcgattctgg ctggaggata
     5221 tgtcatgagt gaagcctcgg tacgcgtcat ggtctattgg cgcaaaaagg acagcgatgc
     5281 ggatactttg attttgttgc ccgacctggt gttcaggcgg cgggaatgac aggtcatccc
     5341 gggcgacgcc tgcaccagtc tctccgcgca cattcgcatg gatcctgcgc cgatgagggt
     5401 cgataccgtg cccgcctctt tcggaaggcg gccgccggcc ccgccgtcaa gccttcggaa
     5461 tgcctgaacg gcaattcagt tcggcagaca aagggaaagt cccaacgtgc aaaagtcgcc
     5521 ttgacagcac ggcaatgaaa aggtgcgggc ataatgtgca atgcgacccc gcaatgccga
     5581 gaggcgtata aatcgaagga gatcccctca gatgaacgca tttgacaacg cttttgagag
     5641 gaaatggacg gtcattttcc tcttggaatt ctttttcatc atgatgccct ttccatggtt
     5701 ctacgacctg gagtacacgc cgtggctctt cggcgtgccg cgattcatct actgctggct
     5761 ggcctacggg ctgctggtga taggaacgat tgctctctgg tggcgcagct gcatgaagcg
     5821 tcccgaatac caggaatacg aggactagca tcatgccgta tcaagttgaa acatccaccg
     5881 ttccctttct gatcgtcacg atcagctatc tcgcggtctt gggcttcatc ggctggtggg
     5941 gcaaccgcca aaaccgaaat ctgcaggatt tcttcacgat gtcgggctcg gctggcgccg
     6001 tgctttcagg ccttgcctat ttctcgacgc agtattcgat gtcgaccttc atgggcgtgc
     6061 cgggctcgat ctaccatgta ggctatgcag gcatgagcgt ctcggtgccg ggcatcgcct
     6121 tttcgatggt cattccggcg cttctcgtcg gccgcaggct catcaagctc ggccatcgct
     6181 acaagatgct cacgatggcc gactatcttg ccgaccgtta tgagtcgacc gccatccgcg
     6241 gcctgctcgc catcctgatg ctcatcttcc tcgtgcccat gatgggcgcg cagacgatcg
     6301 gcgcgggcgt gatcttcacg acctacacgg gcgctccgca gtgggtcggc atcgtcgtga
     6361 tgggcgtcac cgtcatcctc tactgcatgg cgggcggcat ccgctcggtc atgatgaccg
     6421 acgtcattca gggcgtgctg atggtggtca cggccgtgac gaccttcttc gtctcgctca
     6481 agctcggcgg cggcctcgag accatcaacg ccaagctcgc gtccgtggac ccgaactacc
     6541 tgaagttccc tggccacaac aactcctatc cctggcagaa ctatgcgtcc atgatcgtca
     6601 tgtggtcctt cttctgcatc ggtcagccgc acctcttcac gaagttcttc acgatgaaga
     6661 actacggtac gatgttcaag gccgtcctcc tcggcacgct cggcatgtgg ttctccgcaa
     6721 gcttcatcga gtggtgcggc gtgacgggcg cggccagttt ccccggcatc ccgggcaagg
     6781 aaagcgactt catagtgccg ctcattctcc aggaaggcct gccgtccatc gtctccgcac
     6841 tgatggtcgc cggcatcttc tcggccggca tgtcgacgat cagctcgctg ctcctcacct
     6901 ccacgtccgc cgtttcgcgc gacctctatc agcgcattct caagcgcgat gccaccgacg
     6961 aagacacgct caagatgtcc cgcgtcgtga ccgtggtgct cggcatcatc gcgatcggca
     7021 tcggcatttt gaagccgggt tcgatcttca cgatcattct ctttgcgttc ggggggctgg
     7081 gcatctgggc cgcgcccatc atctgcggca tgtattggaa gcgcacgacg accacgggcg
     7141 tcttcgcgtc cgtgatcatc ggcgaagcgt tctacttcct gcttctcacc gagttcaagt
     7201 cgctctccat gggcttcaat ccgctcatcg tcgcctggct cgtcaccatg atgatcctca
     7261 tcggcgtgag cctcttcacg aagcccgtgt cggctgaaac catccgccgc cacttcgacg
     7321 atctggacaa gcccgtcaag gcgtaattca agctcaagcg actgagaatt acggtgcgcc
     7381 gagccggctt tcccgcaggg gagggccggt ggcgcgcctt ctgataaagg ataaacatca
     7441 tgcagcttgc acatctcaac tggcaggagg ccgaaaaggt tcttgccgat cctgaaactc
     7501 tcgtcgtcat tcccgtcggt tcgaccgaac agcacggctc cgtaggcccg ctcggcaccg
     7561 actggctcat cccgcaggag ctctgccgcc gtctcgacgg tcgcatggaa aagctcctca
     7621 tcgcgcccgt cattccgttt ggcgtcgcga cgcatcacat caacttcccc ggcacgatcg
     7681 accttggcct tgaaacgatg atcaaggtga tgggtgccgt cttcgaatct ctctacaggc
     7741 acggcgcaag gcgtttcgcg gtcatcaacg gccacggagg caacgatccc gcgatcgaaa
     7801 aggccgcgct cgagatgtat cgcaggggct cccaggtgac gctcgtcaac tggtggggca
     7861 ttgcgcccaa gctcaatccg gactggccca cgggccacgg cgacgctcag gaggctgcgg
     7921 ccgtcatggc cttcgcgccc gagctcgtca agtccgaatg gctcgtcgac aacgtcgtca
     7981 cgccgccttc cgcggacttc aagcagctcc acatcaacac ggttctcttt gagggcgcgc
     8041 ccgtcaaggt gattcgcgaa atccgcgaca ccgtcaacac gggcggtctc ggcggtcttg
     8101 aatccaagtt cgccacgcgc gaatggggcg tcgccatgat ggacggcgtt accgactgga
     8161 tggtccgcta tctcgagacc ttccgcagga tcgacctgcc gaagaagcat tgggactgac
     8221 cttatggctt acgaagacat tcttgccgcc gtggatgcag tgcggctcat ggatagcgtt
     8281 cgcgcgatct gcacgggcga acgtctttca aacgaggcgg aagtccgttc cttcgacgtt
     8341 ctggaaaagc tcttcacgga cgccggttgc cgcgtgacgc gcgaggcgct tccaggctac
     8401 gtcagcacgc ccgaaggcgc agccttcgaa gcgggcggca ttgcttatga agtactcact
     8461 caccccatga cgccttccgc agatggcctc gaggcggatc ttgtctacgt cccggtcgac
     8521 cgaaccgggt cggcatccgc cgaagaggtc tccggcagga tcgttctcac ggacggtctg
     8581 gcaatggagc ccgtcgtgcg cgcccttgag gacaggggcg ccgcaggcgc cgtcttcatc
     8641 acgggcgagg agatccacaa catgatcgtc tcgcgcgtct ggggctcgcc cacgcccgaa
     8701 accctgcacg gctatgtcgg cattcccgtc gcaagcctct cctttgggga cggctctcgg
     8761 ctcagggccc gcctctccga ggcgggcggc acgctgcccg caaggctttc gacccgcgtc
     8821 gaaagccgct ggacggaaat tcccgtcatc acggccgaga tccgcggcgc ggacgaggac
     8881 ttcgtaatgg tgacgggaca cgtcgacagc tgggggctcg gcgcccttga caacgcctcg
     8941 ggcaacgcct gcgccgtcga gctcgcgcgc atcctcgcgc cgctcgcctc cgacatgcgc
     9001 cgaagcgtgc gcttcgtcct ctggtcgggg cactcccacg gacgctacgc aggctcgacc
     9061 gcctggtgcg acgcgcactt cgaggagctc gaaaggcact gtcttctcaa cgtcaattcg
     9121 gactgcctcg gcgggcgcgg cgcaacggtc atgaccgagt cgcccgccat ggcctcgacg
     9181 cgaggcctcg cgcgcacggc gctgcgcgag gcggccggcg tcacggactg gcgcggctgc
     9241 cgtttttcgc gcagctgcga ccaaagcttc tggggcgcag gcgtgccctc gatcttctct
     9301 caggtctccg agcagccgcc ctttgagggc gccgccgcgg acgcctttgg caagatgttc
     9361 ggcagcggca ggacgggcgg tttcggcgcc tggtggcaca cgaggaccga cacgcccgac
     9421 aagctcgacc cggaaaacct cgcccgagac gtgcgcgtct tcgcgagcgt gctcgcgcat
     9481 gcgctctttg acgaaagact gcccgtcgat gcggcggccg aggtgctcga actcagggac
     9541 gagctcaagg cgtggcagga aaaggcgggc gactcgctcg acctctccga ggtccttgcg
     9601 cgactcgatc ttcttgctga ggctctcaag gctgcggccc gctcggacga tccgaagcgc
     9661 ttcgaaaagc gttcccgcag ggtgctctcc gagatcatcc cgctcgccta tgtcgagggc
     9721 tcggtctact cccatgacga ggcactgcgc gcgccgccag tgccgatgct gagactcgtc
     9781 gacgaacttg cgacactctc cgggcacgag agaaacgccg ccctcgtcag tcttcgccgt
     9841 gtcgtgaacc gcctcaaggc gggcgccgca cgggcgcttg atgtcgctcg ggcctgacgg
     9901 cggcaagtgc ggacctgaag gcaggatgga ttgcggacgc atgcgctgca gcccgtcttt
     9961 tgtttgaact gatctccgtg cattccccac ccatgcgaag catgggtggg ggcaagcggg
    10021 ctgaaaaggc gaaggactca gccgagcgcc gtgtcgagcg tcatcatcag gatgaagccc
    10081 gtgatcacgg cgagcgtgcc cgcatcggaa tgcttagaga gatgcgcggc gggaatgagt
    10141 tcctccacca ccacgtagag catggcgcct gctgcaaagg ccagcatgaa gggcatggct
    10201 ggcatcacgg tctggagtac cagcacgacg agaaggccga agactggctc gacgattccc
    10261 gagaaggcgc cgaagagaaa ggcgcgcatt ctgctcatgc cttgtcctgc aagcggaagc
    10321 gagacggcgg cgccttcggg aatgttctgg atgccgatgc cgagcgcaag cgcgagtgca
    10381 gaggagagct cggcgggctc gcccgagagg gcggcggagc ccgcgacgag gcccaacgaa
    10441 ccgccttcag gcacgttgtg cagcgtgatc gcaatgaaga gcagcatcgc tcggtgcatc
    10501 gaggtcttcg gcccctcggg ccgggcggcg cccgggtgca ggtgcggaag cgccgtgtcg
    10561 aggagtttca ggaagagcac gcccgccgcc acgcccgaac ccgccgcaag ccatggcgtg
    10621 ccgccctgtg cctgcgccat ctcctgcgcg ggaatgatca ggctccagac tgacgccgca
    10681 atcatgatgc ccgcggcaaa gccgagcgag agcgtttcgg cgagctccgc gccgcgacct
    10741 cgcccgttcc ccggcttcat gaagaagacg gccgcggccc ccgctgccgt gcagagaaag
    10801 gtgaagcccg tcgcaagggt ggtctgggcg agcgcgttga gaagttctga cgtcatgggc
    10861 tttcggatcg aattgagtga gaatgattct caagtataga gccttgcggg actgcgtgcg
    10921 cattttgagc ggtccctttt tgcaaatcgt ttcaggagac ggcttccgag ccgagccatc
    10981 gtaaaattcc tcttcattgc gggtgcagac agcctataaa ttgctggcta acaaatggcg
    11041 tccgccgtca cgatacgagg atttgactac atgaccaagc gactcatgcc gactcgcgag
    11101 aacctgcgca gggtgcacag gggcgccgcg atgagcgaag aggacattgc gggcgtcgaa
    11161 ctgatgcttt acctgatccg agctgccgac gtggtgcgcg aatcggtcta ttcgggcctc
    11221 cgggagcggg agctttccga agggaagttc gcgcttctga tgacgctctt cgatgcggac
    11281 ggccccgtgc ctgtcgttga gctggccaag cgcatcggcg tctccgctcc gaccacctcc
    11341 gtcatggtga cccgcatgct gcaggccgag aaccccctca tcgccaaggt ccagtcgaag
    11401 gccgacgccc gttgctggct catcgaactc accgacgagg gcagggcgct catcacggcc
    11461 gagcttcccg cgcataccgc cagagtggcc gacttcgcga aatgcctgtc gggcgatgag
    11521 tgcgagcaga tgatcgacat gctcaagaag ctcgtcgccg acgattgatg ccggagcctg
    11581 ccgtgtgaat cctccccgac caattattaa ttggacgggg cttccctgcc ttttaccggc
    11641 aaggactgcc tgagcacaga tagcgggcaa actcagaaat ctgcgctcac atggagcgca
    11701 gctccgcgtt aacccgaatt aataagatgg tcagctccag aaggggcatc aaacaccgga
    11761 gaccatcaat gcagaacttg acaacctgca aacaaaagaa gcactctctc ttaaatctcg
    11821 ctttcggccg agaataccac ggcatctaag tgccaaagag tcgactaaaa ctcatacgat
    11881 aagaggagtg cttcgtcatg aatagtacac aagttttcga gaaacgcaag atcgccgttg
    11941 cgattggcat cgacctggcc aaagaccatt gcgacgttgt tgcatacaac gctgacaaca
    12001 agatctgctt caccaaagcc aacatgacct atccgaagct tatggaatgg cttgcaaacg
    12061 cacaacctgc cgtcgtcttg atggagtcgt gcaaaggttg ccatgttcgc gcacgcgaca
    12121 ttgccgatct cggccacgat actcgtctcg tcaaaggggc tgatgtcaag gcattgcgca
    12181 atgtttgtca aaagaacgac attcgagatg cggactacat tgccaggctc ctctacgtcc
    12241 ccggcaccaa attcgtcttc gtgaagaatc aacgccaaca gtcgctgcaa ttcctgcaga
    12301 atgaatacaa gagtttccag gagttaagga tccggatggg gaatcagatc cacgccggtc
    12361 tcgaagagtt cggctgtcct gcacccaagt ctcaacagtt cgttcaaagc cgcatgatgg
    12421 ctcatctgga gaagaatgcg gaactgatcc ctgaggaagt gatggcttct tttctccgtc
    12481 gtcgcgagat gtggctcaac ctgttcaagc aagaggagga cgccaaaaag cgcatggagg
    12541 agatcgccaa gacggatgac gacaccaaac gcatgatgac cgtaccgggg gttggtgctc
    12601 agacggcagt cggaatgttg gtgcacatcg gaggcgacat cgggcggttc aaaaacagcg
    12661 ggcagtttgc cgcctccatt gggcttgttc cgaagcagaa catcaccggt ggcaacacca
    12721 cgttgaatgg catcaccaaa cgcggcccca agaggctgcg ctcgaatctg gtgcaatgcg
    12781 gacaggtgat actcatgcac gcggataagc taaaagggaa ttttggcgac tgggtccgaa
    12841 aaatgcgaga cagcggcaag aaacgaggcg tcattgcctg tgcaattgca gccaagatcg
    12901 cccgaatcat ttatcgcctg atgaaggaca aggttgatta cacaccggta ccgagccgtt
    12961 aaaggtacgg atcgcgcata gcgatcatcg gcaaaatacc gccaaggact acgaaagcaa
    13021 ctactagtga tgaatcgtgc tcaacacaat agagtgatgc tgagacactc gatgtgatat
    13081 cacataggta gcgtcaatca cgccgaattt ataaagcacc tctattgttc cgaatctcac
    13141 tatgtcggcg gaggcttagg aatgaatgcc tcctgaccgc ggatgtattt cagtgaagcc
    13201 ctttcttcaa cttcgattat tgttgaaagc ggttcttctg gtagaagtct gaacgaaatc
    13261 actagcgcgt tccgcgctaa agatttggac gggatcgaag actgatgaca gctaactgtg
    13321 cgcgagtcga tggcagctgt tgagcgtctc tctaagcgcc ccccaaccag ggaagatggg
    13381 aatttaacct attcgtgcgc taactcgcga cgccggagtt tcagacaatc gatcaacgtt
    13441 ttcaagaatg tggcttcgcc acggaattga atgagggcgc gcgttttggg ttgcgcgccc
    13501 tcagggaaga ttgtacccaa aatatatgat ttatcccttg caattgggga gctgaccatg
    13561 tatagagtgt ccagggtgcg tgtgatcctc ttggcacatg acggaacttt agccgcaatg
    13621 caccgccatc tcgttgccga aatggacttc tcgtactccc tga
//
LOCUS       DBMDTI010000030        13578 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1391_length_13578_cov_2.391630, whole genome shotgun sequence.
ACCESSION   DBMDTI010000030 DBMDTI010000000
VERSION     DBMDTI010000030.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 13578)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13578)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13578
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1391_length_13578_cov_2.391630"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="ACZPB6_02885"
     CDS             complement(<1..715)
                     /locus_tag="ACZPB6_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR1183457.1"
                     /translation="MKARLMILSAFAALPMLASAAAFTPAELDKAVASLPKANVMGGF
                     AVHSKQFCGSCHGPNGIAPTANWPHVAGQPFEVTAKALLDYRDGRRTGGAQAAIMTAA
                     AKRLTDQQIADVSALYEMLPGPDGKTFDVGKKGRTPVEGVDVARLVRKGNPARAITPC
                     AACHGMTGKGRSNGKVPVLHGQNPTYLAAQLRDYRDGRRDNDVLKEMRFFAQKLTDDE
                     INALADFYAGETGWPGVKPA"
     gene            complement(731..2098)
                     /locus_tag="ACZPB6_02890"
     CDS             complement(731..2098)
                     /locus_tag="ACZPB6_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HIR1183458.1"
                     /translation="MKAINIQRRRLMTSLALSALAAAPGASWAQTAAKALAPARGARI
                     VVVGAGVGGATAAKYLKLFNKNLDVTLIDRNPNFIRHYGSSELITGAVTMEDLTVSYD
                     ALSDRHGVRVVRDTIVGLDADRRTVIGEKGRYAYDKLIVSPGIELLYEGIPGYSAELA
                     ATKIPSGWIAGPQTQLLADQLKAMPQGGTFCLVAPPNPYRCPPGPYERAALVTEWCAK
                     HNPTAKIIVTDPKNNFVTDETMLLGWNRLYGFSIPADYKARLDKYASPARPDCRLEWV
                     QEKQGGRPLAIDASAMTVTTEAGVIKADVINVIPPMRAANVALAMGLADKTGFCPIDR
                     RTFESTIIPDVYVLGDASIADAMPKSGFSANTQAKTTARAIVEELAGREITEPVWSNT
                     CYALAGEDYGLFVADVFRIMDGKIGRTNTRARYQYLKAQPAEQALAAQYLHSWMNTIT
                     ADSFG"
     gene            complement(2095..2436)
                     /locus_tag="ACZPB6_02895"
     CDS             complement(2095..2436)
                     /locus_tag="ACZPB6_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012264.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR1183459.1"
                     /translation="MNIIKTTCAAVLILAAAASQAAPDPAKLSGNPTGEALAHTCAAC
                     HGTYGELKGEHFVPLAGMDEGEFITSMKEFRSHKRPSSIMSHIAEGYSDEEFERMARY
                     FNGIRIKGDVK"
     gene            complement(2450..3697)
                     /locus_tag="ACZPB6_02900"
     CDS             complement(2450..3697)
                     /locus_tag="ACZPB6_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183460.1"
                     /translation="MNDMMLMALTDPAGAPAHPLIFLVLGVVTFALHLTAVNVMLGTL
                     GLAAWGAFSKNPYWQRLAGALGTTAKVAVSVAIVLGVAPLLFVQVIYDPFWYTSNVIS
                     AWWLLGFLAILTVAYLALYRFYGLNHRYEADGRPVHLEGAPKGGVWLAGSLVLMLVCG
                     LIMHAVVNQSLLPAEWMNWYAPNGEIEPYGRSLHSVTPARLLFFLLLSLPVTAGWLFG
                     MRRYLLSSGETDYGYVDFIEGLAHGMARVGSVLVLLAGAAWTATLPETMSWFAGSVWM
                     WIGLVPLAYFGAMSFIQKKRVLCIFCNYMAFGMTLVMTIVLAALREVLRFVTFLEGSG
                     YDALAYKITMDWPSTVIFFTTFLVVGGLNLTYLLSLAWKSGQTEGVYQPSAALSRVGV
                     AAIASLALWVAGYFAIGIAVVNG"
     gene            complement(3694..5061)
                     /locus_tag="ACZPB6_02905"
     CDS             complement(3694..5061)
                     /locus_tag="ACZPB6_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIR1183461.1"
                     /translation="MQQELVMLGLYPTWFEPGVGSGWIIGFIATIHVLFSHASVGGAV
                     LFAWLANFAVRRDKPAYLAFIRRYGLFLLIFSYVLGSITGPGIWFAATIASPRGISAL
                     IHSFVWLWASEWVFFVIEVIGVYLLVYLAGRVDPRTHTRISIIFGLASVATLLVIVGI
                     LSFMLWPGQADWHQTGGVLNAFFGENTFAQMTARFMFMLTITGVVGGMVAGRIADSEE
                     KAMIARVLSGAGILGVIGGWLAFRWYMTTLPDIAYETMATRLPESFGMMMAASIGVSV
                     LYFLVTAWKPQVLRPWLAGVMTVVILVLGLAPEETAREIVRKPWVAGQYVYGNQLVGR
                     DVPALGIRSELPLMAEKGVLATHPFMPEHLRKVTPENEAEAGRALALTLCSNCHSLTS
                     TGMRPLERFFPADADRAFLADYLGAGLYRGHSVYMPPVPLPEAERGALAAYIESILPK
                     KGAAK"
     gene            5663..6661
                     /locus_tag="ACZPB6_02910"
     CDS             5663..6661
                     /locus_tag="ACZPB6_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLAC1 family transporter"
                     /protein_id="HIR1183462.1"
                     /translation="MERFIRPMAPGWFASVMGTAVTSLALTLASLAAADAGPALAAPL
                     ALAASALHWLAIAMMSVLGIAAVYRLIRHGDAVMEQLRHPVEGSFYATFPIAMLVMAA
                     VWTIRGVSPALIAPLWWTGALGTFAVSYVVLFGLFTSERLKLGMVTPAHFIPAVGLVV
                     IPVAGAPLAAASEGVLRELAFALNMTGFGAGVFMYVGLLALTLWVHLAPLSVIPLSML
                     ALLKTTGDASLLRYGSLVAAAFLGAALWWLVLAVSMTIRNRRQGKLPFALSWWAFVFP
                     IGACSVLAYRVSELLALEVLPLVASGLTLLALFVWSAAALGTVRGIRTGAIFAPAL"
     gene            complement(7107..8246)
                     /locus_tag="ACZPB6_02915"
     CDS             complement(7107..8246)
                     /locus_tag="ACZPB6_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024634.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1183463.1"
                     /translation="MPVFEEMMAAARGDGVLDGAPAGAPVLVARRCVRRLHQASSCTK
                     CAEACPVHAVSVKSRSVEIDAASCTGCGACSAVCPAEALYMRTPGASQERAFLERLKD
                     ADMLSIACAEAAGEARRMADLTTACLGTMDMSPVASLAARTGGACTLHVIHGDCRRCA
                     RRGVSSDPLERMRRWEAVLSRVAPEITQEIHAAPKSVDTGRRQFWGRLLRKAELDAGE
                     NIPFDEASLAWLETVPLRMVPPARGRWLEALKSARKKGTEALSGMMEAPGRPGFWAPK
                     IDNEACKVCGLCPTVCPTAALEDEKLNGITLTKVTPSKCIGCGLCVDICFRNAVQMLP
                     VRHPEESLDEKPVVIYAGRAGSGDSPEAVWEDKLKTMISAPVYRT"
     gene            complement(8257..10575)
                     /locus_tag="ACZPB6_02920"
     CDS             complement(8257..10575)
                     /locus_tag="ACZPB6_02920"
                     /inference="COORDINATES: protein motif:HMM:NF019604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1183464.1"
                     /translation="MKRNETSARGASKHAPVKNAGSRRLRSLTAAAFIVMTGIGLAWH
                     TGWGTLSSFGIGSIAEVCPLGALEAMLADRTFLPQAFFGLLVVAAVVVLFGRIFCGWV
                     CPVPLLRRITGMKDERPAEKAGACSSKTAACTPSACASCISGGKGDEVASTDGRTDAD
                     RAGRNPGTGPFWVLGGALASSAVFGFPVFCLICPVGLTFALVIALWRLFEFNEPSLSI
                     VFFAGFLILELFVLRRWCHAFCPLGAVMTILARLNRTFRPAVKLEACAASAGIDCRVC
                     RDACPEGLDPRGGNTPWTDARCTKCRACADACPTHAIHFPAFERKAAARAASSAGEGA
                     VAVKVLLPPRVPEKAIPVEARRTTFAETVEGLSLREAMQEASRCLRCGECVAACPLGN
                     PIPDMMALMAEGRAAEAGRLLVRSGAAPDICGRICPQERLCEGACSLGRSGAPVAIGA
                     VERAAADAALARGAHLERRRPNRKASVAVVGAGPAGLACADMLARLGATVTVIDAHEE
                     AGGLLAHGIPAFKLDGNVLEKRLSVLGKAGVAFRLGTRFDGRIVDEVLARHDAVFVAT
                     GAGRAVMPSFAAEVRTGFVDALAFLKENASGSGNKSTLAGQRAVVLGGGDTALDCART
                     AVRLGASETIVACRRGTEELRASRREVRHALEEGVVFMPHVTPVAAEADADGVLTGLV
                     LENVETGERLSIGTTTAVVAFGQRCVRLERLAAEGVVYDESDRILVDAEGRTGVPKIW
                     AGGDAVRGPALAVEAVADGRRVAFSIAETLGL"
     gene            complement(10572..11057)
                     /locus_tag="ACZPB6_02925"
     CDS             complement(10572..11057)
                     /locus_tag="ACZPB6_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024247.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1183465.1"
                     /translation="MKALCLRCDRCRSVCHTGVIGVGGFAEGLLVMRTPVMNFHGGFC
                     DFCRKCAEVCPTGAIRDFDPETEKIGLAHVTETCIALRTAACTACEEACPYDAITLDA
                     QNRPVVDPGLCNGCGKCEQICPANVYQAYRGGRLRGIVVRPLSAGGQGDAEGDARGGM
                     R"
     gene            complement(11344..12009)
                     /locus_tag="ACZPB6_02930"
     CDS             complement(11344..12009)
                     /locus_tag="ACZPB6_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014651.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorD/DmsD family molecular chaperone"
                     /protein_id="HIR1183466.1"
                     /translation="MNQNEKAALREILAQRESMYRLLARLYKTEADKDLLDGLGAAGF
                     PEEDGGLGEGFRLIADWLRDPKSEDPVTELAVDYAHTFLGAGIADGLVSYPFESVYTS
                     SGRLVMQDAWEDVCRIYREHGLERGEQADMHEDQIGLECEFMAILAGRALEALEADDE
                     QALEASLADQHAFLTKHLLNWVPRFTADVTVTALTDFYRGLAKVTDEFIRMDGGLLED
                     LKA"
     gene            complement(12045..12860)
                     /locus_tag="ACZPB6_02935"
     CDS             complement(12045..12860)
                     /locus_tag="ACZPB6_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183467.1"
                     /translation="MGNDWLLIGFAAFVLTGTGLAAALAAGERKSLPIAVQAWAAAVA
                     LALLAAGGACAVLHLGRPELIFGALAHPSTGIFREFVTTGLTAVVLAAYLVALLRRAE
                     AGTTHMLARAGALLALCTAAAVGSAFYMPWRAGWNTIAIVLPYAAWCLAGGAFAAEVL
                     SEVSDEAEPSERKRSAFAALLSLAALAAYVAATVVSDGAAPVLSRVVGGDLTGLFAGA
                     VVFGSLVPLVCAVPSRGRLLPAACGFISIAAGAVLLQWTVQCLGLPAWQFFAR"
     gene            complement(12881..13480)
                     /locus_tag="ACZPB6_02940"
     CDS             complement(12881..13480)
                     /locus_tag="ACZPB6_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1183468.1"
                     /translation="MTHKTLVIDLDRCIGCFACEMACKQENNVPLGVHYNKVLSIGPM
                     GKFPDLTQYFLPTVCQHCKDAPCVKVCPTGATYQTEDGQVLVNKEKCIGCKMCMAACP
                     YGARSYNPEAKVVEKCTLCHHLQEVGDQPACVKVCCAKARFFGDVDDPESDVSKVLAA
                     AGTENVHSMPDSGNHPTVRYILHKKTGEWQVNPPKMVEN"
BASE COUNT         2336 a         4403 c         4488 g         2351 t
ORIGIN      
        1 tggcgggctt gacgcccggc caaccggttt cgcccgcgta gaaatcggcg agcgcgttga
       61 tttcatcgtc ggtgagcttc tgcgcgaaga agcgcatttc cttcaggacg tcgttgtcgc
      121 ggcgtccgtc gcgatagtcg cgaagctgtg cggcgagata cgtcgggttc tgtccgtgca
      181 ggacgggcac cttgccgttc gagcggccct tgccggtcat gccgtggcag gcggcgcagg
      241 gcgtgatcgc gcgggcggga ttacccttgc gcacgagcct tgcgacgtcg acgccctcga
      301 cgggcgtgcg acccttcttg cccacgtcaa acgttttgcc gtcggggccg gggagcatct
      361 cgtagagcgc cgagacgtcg gcgatctgct ggtcggtgag gcgcttggcg gcagccgtca
      421 tgatcgcggc ctgggcgccg cccgtgcgtc ggccgtcgcg gtagtcgagg agcgccttgg
      481 cggtgacctc gaagggctgg cccgcgacgt gcggccagtt ggcggtgggt gcaatgccgt
      541 tgggaccgtg gcaggagccg cagaactgct tgctgtgcac ggcgaatccg cccatgacgt
      601 tggccttcgg gagcgaagcc acggccttgt cgagttcggc gggcgtgaag gctgcggccg
      661 aggcgagcat cgggagggcg gcgaacgcgc tcaaaatcat cagtcttgct ttcattgttc
      721 ttctccggga tcagccgaaa ctgtcggcgg tgatcgtgtt catccagctg tgcaggtact
      781 gggcggcaag ggcctgctcg gcaggctggg ccttgaggta ctggtagcgg gcgcgcgtgt
      841 tggtgcggcc gatctttccg tccatgatgc ggaagacgtc ggcgacgaag aggccgtagt
      901 cctcgcccgc caacgcgtag caggtgttgg accagacggg ctcggtgatc tcgcggccgg
      961 ctagctcctc gacgatcgcg cgcgcggtag tcttggcctg ggtgttggcc gagaagcccg
     1021 acttgggcat cgcgtcggcg atcgaggcgt cgccgagcac atagacatca ggaatgatcg
     1081 tgctctcgaa cgtacggcgg tcgatcgggc agaagcccgt cttgtcggca aggcccatcg
     1141 cgagcgcaac gttggcggcg cgcatcggcg ggatgacgtt gatgacgtcg gccttgatga
     1201 cgcccgcctc cgtcgtcacg gtcatggcgc tcgcatcgat ggcgagcggg cgcccgccct
     1261 gcttttcctg cacccactcg aggcggcagt cgggacgtgc gggcgaggcg tacttgtcga
     1321 gcctggcctt gtagtccgcg gggatcgaga agccgtagag gcggttccag ccgaggagca
     1381 tcgtctcgtc ggtcacgaag ttgttcttcg ggtcggtgac gatgatcttc gcggtcgggt
     1441 tgtgcttcgc gcaccactcg gtcacgagcg cggcgcgctc gtaggggccg ggcgggcagc
     1501 ggtacggatt aggcggtgcg acgaggcaga aggtgccgcc ctggggcatg gccttgagct
     1561 ggtcggcgag gagctgcgtc tgcgggcccg cgatccagcc gctcgggatc ttcgtcgcgg
     1621 cgagctcggc gctgtagccc gggatgcctt cgtaaagcag ctcgatgccg ggcgagacga
     1681 tgagcttgtc gtaggcataa cggccctttt cgccgatgac ggtcctgcgg tccgcgtcga
     1741 ggccgacgat cgtgtcccga accacgcgca caccgtggcg gtcggagagc gcgtcgtagg
     1801 agaccgtgag gtcctccatc gtgacggcgc ccgtgatgag ctcggacgag ccgtagtggc
     1861 ggatgaagtt cgggttgcgg tcgatgagcg tgacgtcaag attcttgttg aagagcttga
     1921 ggtacttggc ggccgtggcg ccgcccacgc ctgcgcccac cacgacgatc ctcgcgccgc
     1981 gggcgggtgc gagggccttg gcggcagtct gggcccagga ggcgccgggc gccgcagcga
     2041 gcgcggagag cgcgagcgag gtcatgagcc ggcggcgctg gatgttgatg gctttcattt
     2101 cacgtctccc ttgatgcgga tgccgttgaa gtagcgggcc atgcgttcga actcctcatc
     2161 gctgtaccct tcggcgatgt ggctcatgat cgaggacggg cgcttgtggg agcggaactc
     2221 cttcatggag gtgatgaact ccccttcgtc catgcctgcg agcggcacga agtgctcgcc
     2281 cttcagttcc ccgtacgtcc cgtggcaggc ggcgcaggtg tgcgcgagcg cctcgcccgt
     2341 gggattgccc gaaagcttcg cgggatccgg cgcggcctgg gaggccgcag cggcgaggat
     2401 caggaccgcg gcgcatgtcg tcttgatgat gttcatgatc agtgactcct taaccgttga
     2461 cgacggcgat gccgatcgcg aagtagcctg cgacccagag ggcgagcgag gcgatggcgg
     2521 cgacgcccac gcgcgagagc gcggcggagg gctggtagac gccttcggtc tggccgctct
     2581 tccaggcgag cgagagaagg tacgtcaggt tgaggccgcc gacgacgagg aaggtcgtga
     2641 agaagatcac ggtgctcggc cagtccatgg tgatcttgta ggcgagcgcg tcgtagcccg
     2701 agccctcgag gaacgtcacg aagcggagca cttcgcgcag cgcggcgagg acgatcgtca
     2761 tgacgagggt catgccgaag gccatgtagt tgcagaagat gcagagcacg cgcttcttct
     2821 ggatgaagct catcgcgccg aagtaggcga gcggaacgag gccgatccac atccagacgg
     2881 agcccgcgaa ccagctcatc gtctcgggca gcgtggccgt ccaggcggcg ccggcgagga
     2941 gcaccaggac ggagcccacg cgggccatgc cgtgggcgag gccctcgatg aagtcgacgt
     3001 agccgtagtc ggtctcgccc gaggagagga ggtagcggcg catgccgaag agccagcctg
     3061 ccgtcacggg aagcgagagg agcaggaaga agagcaggcg agcgggcgtc acggagtgca
     3121 gcgacctgcc gtagggctcg atctcaccgt tcggggcgta ccagttcatc cattcggcag
     3181 gcaggagcga ctggttgacg acggcgtgca tgatgaggcc gcagacgagc atcagcacga
     3241 ggctgccggc gagccagacg ccgcccttcg gggcgccctc gaggtgcacg ggcctgccgt
     3301 cggcttcata gcggtggttg agaccgtaga agcggtagag ggcgaggtag gcgaccgtaa
     3361 ggatggcgag gaagccgagg agccaccagg ccgagatcac gttcgaggtg taccagaacg
     3421 ggtcgtagat gacctgtacg aagaggagcg gcgcgacgcc gagcacgatg gcgaccgaga
     3481 cggcgacctt ggcggtcgtg ccgagggcgc ccgcgaggcg ctgccagtac gggttcttcg
     3541 agaaggcacc ccaggcggcg agcccgaggg tgccgagcat cacgttgacg gccgtgaggt
     3601 gaagggcgaa agtgacgacg ccgagcacca agaagatgag cgggtgtgcg ggcgcgccgg
     3661 cgggatccgt cagagccatg agcatcatgt cattcatttg gcggctcctt tcttgggaag
     3721 gatggattca atgtaggctg caagggcgcc gcgctcggct tcgggaagcg ggacgggcgg
     3781 catgtagacc gagtggccgc ggtagaggcc cgcaccgagg tagtcggcga ggaaggcgcg
     3841 gtcggcatcg gccggaaaga agcgctcgag ggggcgcatg ccggtggacg tgagcgagtg
     3901 gcagttgctg cagagcgtga gtgcaagcgc acgaccggcc tcggcctcgt tttcaggcgt
     3961 caccttgcga aggtgctcgg gcatgaaggg gtgcgtggcg agaacgccct tctcggccat
     4021 gagcggaagt tcggagcgga tgcccagcgc ggggacgtcc cggcccacga gctggttgcc
     4081 gtagacgtac tggccggcga cccagggctt gcgcacgatt tcgcgggcgg tctcctcggg
     4141 agcgaggccg agcacgagga tgacgaccgt catcacgccg gcgagccagg gacggagcac
     4201 ctgcggcttc cacgcggtga cgaggaagta gaggacggag acgccgatgg aggcggccat
     4261 catcatgccg aagctttcgg gaagcctcgt cgccatcgtc tcgtaggcga tgtcggggag
     4321 cgtggtcatg taccagcgga aggcgagcca gccgccgatg acgccgagga tgccggcgcc
     4381 cgacaggacg cgggcgatca tggccttttc ctcgctgtcg gcgatgcggc cagcgaccat
     4441 gccgccgacg acgcccgtga tggtgagcat gaacatgaag cgggccgtca tctgggcgaa
     4501 ggtgttctca ccgaagaagg cgttgagcac gccgcccgtc tggtgccagt cggcctggcc
     4561 cggccagagc atgaaggaga ggatgccgac gatcacgagg agcgtcgcga cggacgccag
     4621 cccgaagatg atcgagatgc gggtgtgggt gcgcggatcc acacggcccg ccagatagac
     4681 gagcaggtag acgccgatca cctcaatgac gaagaacacc cattcgctcg cccagagcca
     4741 gacgaaggag tggatgagcg ccgagatccc gcggggggac gcgatcgtgg cggcgaacca
     4801 gatgcccgga cccgtgatgg agccgagcac gtagctgaag atgaggagga agaggccgta
     4861 gcggcggatg aaggcgaggt aggcgggctt gtccctcctc accgcaaagt tggcgagcca
     4921 ggcgaaaagc accgcgccgc cgacggaggc gtgagagaag aggacgtgga tcgtggcgat
     4981 gaagccgatg atccagccgc tgcccacgcc gggttcgaac caagtcggat agagtccgag
     5041 catgacgagc tcctgttgca taaatggttt ttaattgctt tggagaggcg cgcccgccct
     5101 tgcgcttcgg cctggggatc cggggtgctc gtaaaatcaa ggcaaagcgc cctctccgac
     5161 ttgattcgat tgtagttttc cctcatccgc agccgcattg tgggttaacc ctaagtcaac
     5221 gtttttcaag aacgttcatc catccacgcc cccaattgag aatgattcct aaacgaaact
     5281 gccacaggca ttttcagggg atctaatcac aaagtatcgg atttcgtcgg ctttagtagc
     5341 taactcactc atgcttttgc accttccgtt ttcagagcat tttagtcttg cattatcgcc
     5401 ccattagtct cgatcgcctt ctgcgaagaa gacgaacgtc gcgccacgcc ggcgccgacg
     5461 tttccgtcct tttgttttcc gcttcaaacg cgcctctaaa aggcgtttct gaaaggagaa
     5521 gcatcaggag ccttcaaaat cacgttccat gaatttgacg agcaacgccc ttttcgtcta
     5581 cgattcactc caaccatttt tagagaatgg cagcaaccac aggaccgcac gcccttcaac
     5641 aacccttttc tggagtcttc tcatggaacg cttcatccgc ccgatggccc cgggctggtt
     5701 cgcctccgtc atgggcacgg ccgtcaccag cctcgccctc accctcgcct ccctcgcggc
     5761 cgcggacgcg ggtcccgcgc tcgccgcccc cttggctctg gcggcctccg ccctgcactg
     5821 gcttgcaatt gctatgatgt cggtgcttgg cattgccgcc gtctatcgtc tcatccgtca
     5881 cggggacgcc gtgatggagc agctgcgcca ccccgtcgag gggagcttct atgcgacctt
     5941 cccgatcgcg atgctggtga tggcggccgt gtggacgatc cgcggggtct cgcccgccct
     6001 gatcgcgccg ctctggtgga cgggtgcgct gggcaccttt gccgtgagct acgtggtgct
     6061 cttcgggctc ttcacgagcg agcgcctcaa gctcggcatg gtgacgcccg cgcacttcat
     6121 cccggccgtg ggcctcgtgg tgatccccgt tgcgggcgcc ccgcttgccg ccgcctccga
     6181 aggcgtgctg cgcgagctcg ccttcgccct caacatgacg ggcttcggcg caggcgtctt
     6241 catgtatgtc ggccttctcg cgctcaccct ctgggtgcac ctggcgccgc tgagcgtcat
     6301 cccgctctcg atgctcgcgc tcctcaagac cacgggcgac gcctcgcttc tgcgctacgg
     6361 ctccctcgtc gcggccgcct tcctcggcgc cgccctctgg tggctcgtgc tcgccgtctc
     6421 catgacgatc cgcaaccgcc gccagggcaa gctcccgttc gcactctcct ggtgggcctt
     6481 cgtcttcccg atcggcgcct gctcggtgct tgcctaccgc gtatccgaac tcctcgcgct
     6541 tgaggtcctg cccctcgtcg cgagcggact cacgctgctc gcgctcttcg tctggtccgc
     6601 cgccgctctc ggcaccgtcc gaggcatccg cacgggcgcc atcttcgcac ccgcactctg
     6661 acgcaggcag cctcgcctcc gccgtctttc cccccccctc agggcccctc cgccaccatg
     6721 gcgcaggggc cctttttcct ttccacgccc cgcgcctcct caatccgcat cattcttcgt
     6781 tttcaccgaa cgtcccctcc cctaattccg gcgcatttgg cgccgtttgc ctgtagttcc
     6841 cgcgtcagaa gaccgcctaa tagcccacca ctatcgcatt tcatctcacg atttctcgtc
     6901 agattgacca catcaacatt cggcatttcc tccgattgaa tgcctcatca ttccgcaaaa
     6961 catccgaacg tacagatctg cctgctcacc gtgctcttct tctgaagcgc ttctgacgcg
     7021 tgaagcccaa gcgggcccgt cggcaatgcc acgggcccgc ttttctcatc tattttcagt
     7081 cattcggcaa tggacgggac gacggatcag gtgcggtaga cgggcgcgct gatcatcgtc
     7141 ttgagcttgt cctcccagac ggcctcgggc gaatcgcccg aaccggctcg tccggcgtag
     7201 atcacgacgg gcttttcgtc gagcgactcc tcgggatgtc tgacggggag catctgcacg
     7261 gcgttcctga agcagatgtc gacgcagaga ccgcaaccga tgcatttcga gggcgtcacc
     7321 ttcgtgagcg tgatgccgtt gagcttttca tcctcaagcg ccgcggtcgg gcagaccgtc
     7381 ggacacagtc cgcagacctt gcaggcctcg ttgtcgatct tcggcgccca aaagccggga
     7441 cgccccggcg cctccatcat gcccgacaga gcttcagtcc cctttttcct tgcgctcttc
     7501 agagcctcga gccagcggcc ccttgccggc ggcaccatgc gaagaggcac cgtctcgagc
     7561 caggccagcg aggcctcgtc gaagggaatg ttctcacctg cgtcaagctc cgcctttcga
     7621 agcaggcgtc cccagaactg ccgcctcccc gtatcaacag acttcggcgc cgcatgaatc
     7681 tcctgcgtaa tttcaggggc aacgcgcgaa agcacggcct cccatcttct catccgttca
     7741 aggggatcgg acgagacgcc tcttctcgcg cagcgcctgc agtcgccgtg gatcacgtgg
     7801 agcgtgcagg cgccgccggt tcgagcggca agcgaggcga cgggagacat gtccatggtt
     7861 ccgagacagg ccgtcgtgag atccgccatg cgccttgcct cacccgcagc ctccgcacag
     7921 gcgatcgaca gcatgtcggc gtccttcagg cgctcgagga aggccctttc ctgcgacgcg
     7981 cccggcgtgc gcatgtagag cgcctcggcg ggacagacgg cggaacaggc accgcaaccc
     8041 gtgcaagagg cagcatcgat ctccacgctt ctgctcttca cagaaacggc gtgaacggga
     8101 caggcctcgg cacacttcgt gcacgatgag gcctgatgca gccttctgac gcagcgcctc
     8161 gccacgagca ccggcgctcc cgcaggcgca ccgtcaagca ccccgtcccc acgggccgcc
     8221 gccatcattt cttcgaatac gggcattcac tcctcctcaa agtccaagcg tctccgcaat
     8281 ggaaaacgcc actcttcttc cgtccgccac agcctcaacg gcaagagcgg gtccccgcac
     8341 cgcatcgccg ccggcccaga tcttcggcac gccggttcgg ccctcggcgt caacgagaat
     8401 gcggtccgat tcatcataga cgacgccctc ggcagccaga cgttccaatc gcacgcacct
     8461 ttgaccaaag gccacgacgg ccgtcgtcgt cccgatcgac aagcgttcgc ccgtttcgac
     8521 attctcaagc acgagccccg tcagcacgcc gtccgcatca gcctccgcag cgacaggcgt
     8581 cacgtgcggc atgaagacga cgccttcctc aagagcgtgc ctgacctcgc gccttgatgc
     8641 ccgaagttcc tccgtcccgc gcctgcaggc gacgatcgtc tcggaagcgc cgagcctcac
     8701 ggccgtgcga gcgcagtcaa gcgccgtatc gccgccaccg agcaccacgg cccgctgtcc
     8761 cgccaaagtc gacttgttgc ctgaacccga cgcattctcc ttcaggaagg caagtgcgtc
     8821 gacgaatccc gtgcgcacct ctgccgcgaa ggacggcatg acggcgcgtc ccgcgcctgt
     8881 cgccacgaat acggcgtcat gacgggcaag cacctcgtcg acgatcctgc cgtcgaaacg
     8941 cgtgccaagc ctgaaggcga cgcccgcctt tccgagcacg gacaggcgct tctcaagcac
     9001 gttcccatca agcttgaagg cggggatgcc atgggcgagc agtccgcccg cctcttcgtg
     9061 cgcgtcgatg acggtcacgg tcgcgccgag tctggcgagc atgtcggcgc aggcaaggcc
     9121 tgcgggaccc gcgcccacga ccgcaacgga ggcctttctg ttcggacggc ggcgctcaag
     9181 gtgcgcacct ctggcaagcg ccgcatccgc ggctgcgcgc tcgacggctc cgatggcaac
     9241 gggggcgccg gagcgaccga gcgagcatgc gccttcgcaa agtcgctcct gaggacagat
     9301 gcgtccgcag atgtccggag cggcgcccga cctgacaagc agacgccctg cctcggcagc
     9361 cctgccttca gccatcaggg ccatcatgtc gggaatcgga tttccgagag ggcaggccgc
     9421 gacgcactcg ccgcagcgca ggcacctcga cgcctcctgc atggcctcgc gaagggagag
     9481 cccctcgacc gtctcggcga aggtcgtcct tctggcctca acgggaatcg ccttttcagg
     9541 aacacgtggc ggcaggagca ccttcaccgc tacggcacct tcacccgcag acgaagccgc
     9601 tctggcggcg gccttcctct cgaaggcggg gaaatgaatg gcgtgcgtcg ggcaggcgtc
     9661 cgcgcatgct cggcacttcg tgcacctggc gtcggtccac ggcgtgttgc cgcccctcgg
     9721 atcgagtccc tcggggcatg catcgcggca gacgcggcag tcgatgcctg ccgaggctgc
     9781 acaagcctcg agcttcacgg caggacggaa ggtcctgttg aggcgcgcga gaatcgtcat
     9841 gacggcgccg agcggacaga atgcatggca ccagcggcga agcacgaaca actcaagaat
     9901 gagaaagccc gcaaagaaga cgatggagag cgaaggctcg ttgaattcga aaagacgcca
     9961 gagtgcgatc acgagcgcaa acgtgagccc tacgggacaa atgaggcaga agacgggaaa
    10021 tccgaagacg gcggaagaag ccagtgcgcc tccgagcacc caaaaggggc ccgtgcccgg
    10081 attgcgtccc gcccgatcgg catccgtccg cccgtcagtc gacgcgactt cgtcgccctt
    10141 tcctcctgag atgcaggatg cgcacgcaga cggcgtgcag gccgcggttt tcgacgagca
    10201 cgcccccgcc ttttcggcgg gccgttcgtc cttcatgccc gtgatgcggc gcagaagcgg
    10261 cacgggacag acccagccgc agaagatccg tccgaaaagc acgacgacgg cagcgacgac
    10321 caacagtccg aagaaggcct gcggcaggaa ggtcctgtcg gcgagcatcg cctcgagcgc
    10381 accgagcgga caaacttcgg cgatgctgcc gatgccgaag gacgacaggg tgccccagcc
    10441 cgtgtgccag gcaaggccga tgcccgtcat gacgatgaat gcggctgcgg tgaggctgcg
    10501 aaggcgcctg cttcccgcgt tcttcacggg agcgtgcttc gaggcgccgc gtgcggacgt
    10561 ttcatttctc ttcatctcat gcctcctctt gcgtcgccct ctgcatcgcc ctgaccgccg
    10621 gcagaaagcg ggcgcacgac gatgccgcgc aggcggcctc ccctgtaggc ctgataaacg
    10681 ttggcggggc agatctgctc gcacttgccg cagccgttgc agagtccagg atcgaccacg
    10741 ggcctgttct gcgcatcaag cgtgattgcg tcataggggc acgcctcctc gcaggccgtg
    10801 caggccgcgg ttctgagcgc gatgcaggtc tcggtcacat gagcaagacc tatcttctcc
    10861 gtttcgggat caaagtccct gatggcgccc gtcggacaga cttcggcgca cttgcggcag
    10921 aagtcgcaga agccgccgtg gaagttcatg acgggcgttc gcatgacgag gagcccctca
    10981 gcgaacccgc ccacgccgat gacgcccgtg tggcagacgc tccgacaccg gtcgcagcga
    11041 agacagagcg ccttcatgcg ctcctccgcc gatccgggag gcctgacaag aggcggcgtc
    11101 tcgccttgtg cggcggcccc gagtgcaaaa agaccggcca cgccggccgc ccctgcaatg
    11161 aagccccggc ggttcgtcgt ccggtccttg agtgccggcg tccgggccgg ttccggattt
    11221 cttcttttca taagcccgtc tccgtcatgc gcggacgcgg gtcggacgcc cgcgcatgtc
    11281 ctttccgtat tccgtagctg ggacttcggg agtcccgtcc gccgcggacc gccggacgtc
    11341 gtctcaggcc ttcaggtcct cgagaagtcc gccgtccatg cggatgaatt cgtccgtgac
    11401 ctttgcgagc ccgcggtaga agtccgtgag cgcagtgacc gtcacgtcgg ccgtgaagcg
    11461 aggcacccag ttgaggagat gcttcgtgag gaatgcgtgc tgatcggcaa gcgacgcctc
    11521 aagcgcctgc tcgtcgtccg cctcaagagc ctcaagagcc ctgcccgcaa gaatcgccat
    11581 gaactcgcat tcaaggccga tctggtcctc gtgcatgtcg gcctgctcgc cgcgctcgag
    11641 cccgtgctca cggtagatgc ggcagacgtc ctcccaggcg tcctgcatca cgagtcttcc
    11701 ggaactcgta tagacggatt cgaacggata ggagacgagg ccgtcggcaa tgcccgcacc
    11761 gaggaaggtg tgcgcatagt cgactgcgag ttccgtcacg ggatcctcgg acttcgggtc
    11821 ccggagccag tccgcgatga gcctgaagcc ctcgccaagt ccgccgtcct cctcggggaa
    11881 gcccgctgca ccaagcccgt caagcaggtc cttgtcggcc tcggtcttgt agaggcgcgc
    11941 gagcagcctg tacatgcttt ccctctgggc gaggatttcg cgaagggccg ccttttcgtt
    12001 ctgattcatg atgatgtgcc tttatgtgtt gttgatgcgc cgggtcagcg tgcgaagaac
    12061 tgccaggcag gaaggccgag gcactgcacg gtccactgca ggagcacggc acccgccgca
    12121 atcgagatga agccgcaggc cgcggggagg agtctgccgc gcgaaggaac cgcgcagaca
    12181 agcggcacca gtgagccgaa gacgacggcg cccgcgaaga gccccgtcag atcgccgccc
    12241 accacgcgcg agagcacggg tgcagcgccg tccgagacga cggtcgcggc aacgtaggcc
    12301 gcgagcgccg ccagactgag gagcgcggcg aatgccgaac gcttcctttc ggagggttcc
    12361 gcctcgtccg agacctcgct gagaacctcg gccgcaaagg ctccgcccgc caggcaccag
    12421 gccgcataag gaagcacgat cgcgatcgtg ttccagcctg cgcgccatgg catgtagaag
    12481 gccgagccca cggccgcggc cgtgcatagc gcaaggagcg cgcccgcacg ggcgagcatg
    12541 tgcgtggtgc ccgcctcggc cctgcgcagc aacgcgacga ggtaggccgc gaggacgacg
    12601 gccgtgaggc ccgtcgtcac gaattcgcga aagatccctg tcgagggatg ggcgagcgca
    12661 ccgaagatga gctcgggcct ccccagatgg aggacggcgc atgcgccgcc cgccgcgagg
    12721 agtgcgagag cgacggcggc cgcccacgcc tggactgcga tcggcaggga cttacgctcc
    12781 cctgcagcga gcgcagcggc aaggccggtg cccgtcagga cgaatgcagc aaagccgatc
    12841 agcagccagt cgtttcccat gtcagatcct cccctcgggc tcagttctcg accatcttcg
    12901 gcggattcac ctgccattcg ccagtcttct tgtgaagaat gtagcgcacg gtcgggtggt
    12961 tgccgctgtc gggcatcgag tggacgttct cggtgcctgc ggcggcgaga accttcgaga
    13021 catcgctttc gggatcgtcg acgtcgccga agaagcgcgc cttcgcgcag cagaccttca
    13081 cgcaggcggg ctggtcgccg acctcctgca ggtgatggca gagcgtgcac ttctcaacca
    13141 ccttggcctc ggggttgtag gagcgtgcgc cgtacgggca cgcggccatg cacatcttgc
    13201 agccgatgca cttctccttg ttgacgagga cctgtccgtc ctcggtctgg taggtggcgc
    13261 cggtcgggca gaccttcacg cacggcgcgt ccttgcagtg ctggcagacg gtcgggagga
    13321 agtactgcgt gaggtccggg aacttgccca tcgggccgat cgagagtacc ttgttgtagt
    13381 ggacgccgag cgggacgttg ttttcctgct tgcatgccat ttcgcacgcg aagcacccga
    13441 tgcagcggtc gaggtcgatt accaaagtct tgtgtgtcat gatttagagc tccggattct
    13501 tcgtcgtgtc ggtgggctgc gggagccagg ccttgaactg ctcgggcttc atccagacgc
    13561 cctcgggcgc gccttcgg
//
LOCUS       DBMDTI010000031        13520 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1396_length_13520_cov_2.078098, whole genome shotgun sequence.
ACCESSION   DBMDTI010000031 DBMDTI010000000
VERSION     DBMDTI010000031.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 13520)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13520)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13520
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1396_length_13520_cov_2.078098"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /gene="csm6"
                     /locus_tag="ACZPB6_02945"
     CDS             complement(<1..651)
                     /gene="csm6"
                     /locus_tag="ACZPB6_02945"
                     /inference="COORDINATES: protein motif:HMM:NF021162.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated ring nuclease Csm6"
                     /protein_id="HIR1183469.1"
                     /translation="MADSKRRILLIALGTPPHLLTFTLAALYKESPEAMPTEIRIVTT
                     ELGAEMARRSFFGEGNILDAFWRDYGLSPAAFTEADIHAISSADGEPIADIRTLDDSN
                     RAADLIVKLVRECCEDPDASLHVSIVGGRKSMGMLMGSALTFYGRDRDRISHTLSENE
                     TAPDRRPYPSPEELAANPDVVILADLPFLRLRQILPAMMLSKEYSFSEIVRNSQQAI"
     gene            789..1823
                     /locus_tag="ACZPB6_02950"
     CDS             789..1823
                     /locus_tag="ACZPB6_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024880.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR1183470.1"
                     /translation="MTKLIGLTGAYDFLERMILEPAGARYNPDWKKARENLENSHDEN
                     LDYLGTYFPRTVLESAVIFEHLMRTMPSVKKMLAERDVIRVLDFGCGTGGELLGFLIA
                     LGWEFNWELPAVEVDAIDGNQDALGMMQDIMHLCRDKWDFDLRVRTVTHKAEKSGFAP
                     ANVKLRDDYDVLVTSKCLGELNAVSQTPFLDFACEFFDSIAPEGLIVMMDVTSRQPHN
                     RGNDWTNLQMTGEFDTHRLQFRKHGFRTLFPLVCDGCSGCRSHSRFLQFIFDFSELGL
                     LEYSETKLACRAYCRDKLWQKVRAEVPPARWQISMGVSCGECREKGAGENLAELPGCC
                     VNHKYFARGG"
     gene            1831..2952
                     /locus_tag="ACZPB6_02955"
     CDS             1831..2952
                     /locus_tag="ACZPB6_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HIR1183471.1"
                     /translation="MEIQTPEGRVKAMTPVILSASRSTDIPAYYGQWFMNRLRAGWCA
                     WVNPFNRRPSYVSFRDVKAVVFWTKNPAPFLQHLDELDARGLHYYFQYTLNDYEREGF
                     EPNVPALEKRIETFRRLSERIGPDRVVWRFDPIILSPGTGPRDILMRIWTIGKALRGL
                     TKKLVVSFVDVGAYRKVQANLVRDTDLYTRETVLSAEPDEAQRREICEGLAKIRERWH
                     SEGWDIEISSCAEEADLAAWGITHNRCIDGELMERVFGEDKALVHFLHSGKLPEAPAA
                     GADSLFGDPVIPVVPVSKRRNLKDKGQREACGCIVSKDIGMYDTCMHFCAYCYANASR
                     KTVLENRRRHDPESPFLAGEPGKTNEDAKAENTENGGGR"
     gene            2949..3704
                     /locus_tag="ACZPB6_02960"
     CDS             2949..3704
                     /locus_tag="ACZPB6_02960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HIR1183472.1"
                     /translation="MKLFKVIRTTARGVKFATRVGITVGSNMLQGVANVADVPGRAAK
                     KTARGDWDGLERLAERTARGVGRSIENKCTAAGELFSEAERCIANPDRRFLTKKNAAG
                     VAAVATAAVTVAAGASALDLDVVSDVDVDGLPDADGAGGALASGFAPSDLSVENGVFT
                     GDGQELDRLIQYGEVDGTTHVASEDIVRDIGARDAFLSVHGFDGVPEGYEVHHVVPLS
                     EGGADTPDNMILVSEADHAAITSAHAGFYGWRD"
     gene            complement(3813..4988)
                     /locus_tag="ACZPB6_02965"
     CDS             complement(3813..4988)
                     /locus_tag="ACZPB6_02965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1183473.1"
                     /translation="MTLQELMTRWNGYQIEMRRWFHQHPEESTKEVRTAERIRAELDR
                     AGIPWRPCGLGTGTLARIEGAQPGRTILLRGDIDALSVNEETGLPYASEVPGVMHACG
                     HDCHISMLLTAAMIVNEIRDRLKGTIVFAFQPAEELGLGARAMIEEGALEGVDACFGM
                     HVWSDYPAGTVALRKGPMMASGDQFKIHVRGKSTHGAQPQLGADALIMGAAIAQNLQT
                     IVSRETYPGDTAVVTVGKFHSGTHFNVVAGTAELEGTTRTFSPAVRDRFEEQITRIAR
                     STAEAMRGTADVEYLRIVPVTVNDPGMIDVVTGAAGKIFGDKGVIEADLQMGGEDFAF
                     YQEKVPGAMVLLGVRNEACDAVWPQHHGCYKVDESVLVKGAALHVQTALDFLGVDLQ"
     gene            complement(5072..6274)
                     /locus_tag="ACZPB6_02970"
     CDS             complement(5072..6274)
                     /locus_tag="ACZPB6_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183474.1"
                     /translation="MLMYNFVSALAACLVIILLGEVLSKFTKGWVPSVFISAVLILIA
                     FWTFLPKTLVADSKLLPLGSSVAIFLLITHMGTLFSLERLLQQWKTVVICIAGLAGLV
                     GLCWFVGPLFMDRTLVIAGIPPLAGGIVATTMMQKAALDAGLAAAAVFAIVMYCVQGF
                     AGYPLTAVCLKLEARRQLAAFRAGKVEDKGEDARKVMADALAMPGNESKQGVLATPKA
                     WNTPYFVLLKVSLVAWLAFVLGVLTPVNGAIWALVCGVVFCRLGFLEPNVLSRTGSKD
                     FLFLALIMFVYSGLGDSNPEMMAKLICPMVLLIIIGVVGMAVVAAIVGRFLKISPYLG
                     FATCLTALYGFPFDAIMTERICQEEGADKAEVDFLMSRLFPPMIVGGFVTVTITSVIL
                     AGFFVKLF"
     gene            complement(6664..6900)
                     /locus_tag="ACZPB6_02975"
     CDS             complement(6664..6900)
                     /locus_tag="ACZPB6_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183475.1"
                     /translation="MNRDASPWKRTDDAGRIDSFSRFRLLLRACPRISEQRVLLSSQD
                     VLKSPCIGVLYSTDSGEQVLLSRNKRAENGVFAG"
     gene            7192..7497
                     /locus_tag="ACZPB6_02980"
     CDS             7192..7497
                     /locus_tag="ACZPB6_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024996.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR1183476.1"
                     /translation="MKKSFVALAVLGAFAGTSMAADVTMYGVVDTGLLYQHGEASASI
                     DGIGSASAEVDSFSMEPGTNSASRFGIRGTEDLGNGMTVGFKLRVRHGRPQDGQQAV"
     gene            7490..8392
                     /locus_tag="ACZPB6_02985"
                     /pseudo
     CDS             7490..8392
                     /locus_tag="ACZPB6_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024996.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(8482..9333)
                     /gene="serB"
                     /locus_tag="ACZPB6_02990"
     CDS             complement(8482..9333)
                     /gene="serB"
                     /locus_tag="ACZPB6_02990"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIR1183477.1"
                     /translation="MNDMLLAGDDRAILGFAGRLNFRTKLVRPGFIRLSGVSSKGFPT
                     GLAAMAEESAVDVLLLPPDLSLRRFRVACFDMDSTLIESECIDEMAAFAGAGERISRI
                     TRRAMEGLIPFDESLRQRVAALAGSSAEIVTHAVERAVPTPGALDFVDFLTRHGLATY
                     IITGGFEEIATHVARRFGMTGVVCNRLVLEDGRLTGGVRGPAGGKILDADGKRRALEV
                     LAQVNGASLSETIAGGDGANDLQMIAAAGFGFAYHGKPVVMREAPRGVRFGDFSVLKH
                     CFLEAWA"
     gene            complement(9351..10922)
                     /locus_tag="ACZPB6_02995"
     CDS             complement(9351..10922)
                     /locus_tag="ACZPB6_02995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR1183478.1"
                     /translation="MTSNSSSSAAVRFVIVGFDGLRPDSVEADMPALSRFMASNHSWS
                     HYLADFPTETYVNHPGIFSGFRPTGHGLVANCYWRRDMGGADGVFFGFDLEHVLRHRR
                     EDGLLLVPTMGERLGAAGKTMRVYCANSKGSTRLQHLYADRYPDHVCACVHDLVTSVP
                     DNERRELVEDFGPGVPLEFPDFRGTKLVVDLFFERELPRGLGDVTVLWIGEPDHSSHE
                     FGIDDERTREARRDADRQFARVLEWWETEGRDAGVQLVVMSDHGHGVVRRHFDMKGIL
                     EKAGLSILMGREVLEGADTASADAVLVGTYCAGLWFTNPADLSLQLRARDALMTSPDI
                     GLLFSPSDPEQPCSIEGRIPGTFSERLVFTDGRRSPDLRIVPRGDPETGGLVMAPELP
                     LGAGNHGGLLPQEINAVLAVGGSAFPGSAVHDDPAGHADLAVTIMTLEGLLSGSDMVK
                     PTGRLLDEALPGGPVRRTPAGEAFDLTFGAFHQRIERLSSGGRAYVTCAHRINNDGWT
                     PERGLPEGARTDDDD"
     gene            complement(10933..11748)
                     /locus_tag="ACZPB6_03000"
     CDS             complement(10933..11748)
                     /locus_tag="ACZPB6_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1183479.1"
                     /translation="MNTRPALAIAAGIILAVFFTAGLLAPVLAPYDLEAMDLTARLLM
                     PFESAAHILGTDELGRDIFSRLLYSIRMSFVLAVVGTLLGAVIGTLLGFLAARFGGIV
                     DDAVNCGIDFTASLPFIILALTIIAFLGTDVTVIIVIMAVYGWDRYARLTRNLSRSAY
                     TEGYACALEGLGLPTVSIALRHILPNIASALVVNMTLNFPGMILLETSLSFLGVGVQP
                     PMSSLGTMLGFGRDYLTTAWWIAVIPGLVIVVSTLSMSILGDWVQQKLEPAAR"
     gene            complement(11748..12671)
                     /locus_tag="ACZPB6_03005"
     CDS             complement(11748..12671)
                     /locus_tag="ACZPB6_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1183480.1"
                     /translation="MFAVVFRILLRAAFTLLLLTACVFFGLHMTGDPARIMLGNAADE
                     VALEAFRAQWGLNLPLWQQFLVYLEKFVSLDMGTSYMTGQPVRNVFLDALGPTLSLMI
                     PTAVVTLLIGIPSGVCAALHRNTLLDRSLMVMSVFGYAVPNFFMGVLLLLVFSIQLGW
                     LPSYGNATVWHYIMPIITMATSEAAIFSRYARSAMIDALRLPCVRAARMRGLPERRII
                     WLHALPNAMLSILTILGFFLGTLVAGAVITENVFSWPGVGKLLVQSVASRDIPVVQMI
                     VLVTGASLVTANLLMDLLALVVNPRLRKGDN"
     gene            complement(12675..>13520)
                     /locus_tag="ACZPB6_03010"
     CDS             complement(12675..>13520)
                     /locus_tag="ACZPB6_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR1183481.1"
                     /translation="VGESGCGKSTLARLALGLIEPTEGTVRFKNAPIPGRDSAAWRAQ
                     RASMQMIHQDPYACFDPRLPIIEQVAEPLRHHRGLSKEAAAKAALEVLRAAGLPAHHA
                     GRRPSVMSGGQLQRAAIARAVALEPALLVCDEPVASLDVSIQAQVLELLLQLRNRMKM
                     AMLFVSHNLSVVRCICDRVTVMYLGRIVESGTVEAVFSRPLHPYTKLLMASTPGADAH
                     VVRYYPKGAMPSPIDRPKGCVFASRCPLAEARCRTDVPPLRDDGSGHLSACFSGPSFL
                     LHPEA"
BASE COUNT         2570 a         3932 c         4791 g         2227 t
ORIGIN      
        1 gatcgcctgc tgggagtttc ggacgatctc gctgaaggag tattccttcg agagcatcat
       61 ggcgggaaga atctggcgaa ggcgcaggaa ggggaggtcg gcgaggatca cgacgtccgg
      121 attggcggcg agttcctcgg gagacggata gggcctgcgg tcgggagccg tctcgttttc
      181 ggaaagcgtg tggctgatgc ggtcgcggtc gcgcccgtag aaggtgagcg cgcttcccat
      241 gagcatgccc atgctcttgc gtccgcccac gatcgagacg tggagcgagg catcgggatc
      301 ctcgcagcac tcgcgcacga gcttgacgat gaggtcggcg gcgcggttgc tgtcgtcgag
      361 cgtgcggatg tcggcgatgg gttcgccgtc agcggaggag atcgcatgga tgtcggcctc
      421 ggtgaaggcc gcaggactca ggccgtagtc gcgccagaag gcgtcgagga tattgccttc
      481 tccgaagaag gaccgcctgg ccatctcggc cccgagctcc gtcgtgacga tgcggatttc
      541 ggtcggcatg gcctcgggcg actccttgta gagcgccgcg agcgtgaacg tcagaaggtg
      601 aggtggggtt ccaagagcga tgaggagaat gcgtcttttg ctgtcggcca tggcgatgcg
      661 tcccgaagtc gggtgctgaa agcgatttga tgcatacaac tatagcaaac cgcaaatttg
      721 cggtttcgtg gcgtgaaggc tcccgtgatc agaatgaaac cgtcatcaag gaaaaaagga
      781 gcgcatgcat gaccaagctg atcggactga ccggggcgta tgactttctg gagcgcatga
      841 ttctggagcc ggcgggagcc cgttacaatc ctgactggaa aaaggcgcgc gagaatctgg
      901 aaaactctca cgatgagaat ctggattatc ttggaacgta ctttccgaga accgtccttg
      961 agagcgccgt catcttcgaa catctgatgc ggaccatgcc gtccgtgaag aagatgcttg
     1021 cggagcggga cgtgatccgg gtcctcgact tcggctgcgg cacgggcggc gagcttctgg
     1081 gattcctcat cgcgctcggc tgggagttca actgggagct gcccgcggtg gaagtcgacg
     1141 ccatcgacgg caaccaggac gccctcggca tgatgcagga catcatgcac ctgtgccgcg
     1201 acaagtggga ctttgacctc agggtgagga ctgtcacgca caaggccgag aagtctggat
     1261 ttgcgcctgc gaacgtgaag ctgcgcgacg actacgacgt gctcgtcacg tcgaagtgtc
     1321 tcggcgagct caacgccgtt tcccaaacgc cgttcctcga cttcgcgtgc gagtttttcg
     1381 acagcatcgc tcccgaagga ctgatcgtca tgatggacgt cacgtcgagg cagcctcaca
     1441 atcgcggcaa tgattggacg aacctgcaga tgaccggcga gttcgacacg cacaggctgc
     1501 agttccgcaa gcacggcttc aggacgcttt tcccgctcgt ctgcgacgga tgctcgggat
     1561 gccgcagcca ttcgcgtttt ctgcagttca tcttcgactt cagcgagctc gggcttttgg
     1621 agtactccga gaccaagctg gcatgccgcg cgtactgccg cgacaagctc tggcagaagg
     1681 tgcgtgcaga agtgccgccc gcccgatggc agatatccat gggcgtatcc tgtggggagt
     1741 gcagggagaa aggtgcgggt gagaacctgg ccgaacttcc cggctgctgc gtcaatcaca
     1801 agtactttgc tcggggaggc tgacatgcca atggaaattc aaacgcccga aggccgcgtg
     1861 aaggccatga cgcccgtgat tctgtcagcg agccgctcga ccgacatccc ggcctattat
     1921 ggacagtggt tcatgaaccg cctgcgcgca ggttggtgcg cctgggtgaa tccgttcaac
     1981 cgccgtccct cgtatgtgtc gttcagggac gtgaaggcgg tcgtcttctg gacgaagaac
     2041 ccggcgccct tcctgcagca tctggacgag ctcgatgccc gcggcctgca ctactacttc
     2101 cagtacacgc tcaacgacta cgagcgggag ggcttcgagc ccaacgtgcc cgcgctcgaa
     2161 aagcgcatcg agaccttcag gcggctctcc gagcggatcg gtcccgaccg cgtcgtctgg
     2221 cgcttcgatc cgatcatcct gtcgccgggc accggtccgc gcgacatcct catgaggatc
     2281 tggacgatcg gcaaggcatt gaggggactc acgaagaagc tcgtcgtgag cttcgtcgac
     2341 gtgggcgcct atcgaaaggt ccaggcgaac ctcgtgcgcg acacggatct ctacactcgc
     2401 gagaccgtgc tgtcggccga acccgacgag gcgcagaggc gcgagatctg cgagggcctc
     2461 gccaagatcc gagaacgctg gcattcggaa ggctgggaca ttgagatttc gtcctgtgcc
     2521 gaggaggccg acctcgcggc ctggggcatt acgcacaaca gatgcatcga cggcgagctc
     2581 atggaaagag tgttcggcga ggacaaggcg ctcgtgcact tccttcactc gggcaagctg
     2641 cctgaggcgc ctgccgcagg ggcggactcg ctcttcggag atcccgtgat ccccgtggtt
     2701 ccggtctcca agcgcaggaa cctgaaggac aagggccagc gcgaggcctg cggctgcatc
     2761 gtgagcaagg acatcggcat gtacgacacg tgcatgcact tctgcgccta ctgctatgcg
     2821 aacgcaagca ggaagaccgt gctcgagaac cgcaggcggc acgatcccga gagcccgttc
     2881 ctcgcaggcg agcccgggaa gacgaatgaa gatgcaaagg cggaaaacac ggaaaacgga
     2941 ggcggcaggt gaagctcttc aaggtcatca ggacgactgc gcgcggcgtg aagtttgcga
     3001 cgcgcgtcgg catcacggtc ggcagcaaca tgctccaggg cgtcgcgaac gtcgcggatg
     3061 tgccgggcag ggcggcgaag aagaccgcca gaggagactg ggacggactt gaacgccttg
     3121 cggaaaggac ggcccggggc gtcggccgct cgatcgagaa caagtgcacg gcggcgggcg
     3181 agctcttcag cgaggccgag cgctgcatcg cgaatcccga caggcggttt ctgacgaaga
     3241 agaacgccgc cggcgttgcg gccgtggcga cggctgcggt cacggtggcg gcgggcgcct
     3301 cggcgctgga tctggatgtc gtctcagacg tggacgtcga cggacttccc gatgccgacg
     3361 gtgcgggcgg tgcgcttgca tcgggcttcg cgccttcgga tctgtcggtt gagaacggcg
     3421 tcttcacggg cgacggtcag gaactcgacc gcctgatcca atacggggag gtcgacggca
     3481 cgacccacgt cgcctcggaa gacatcgtgc gcgacatcgg cgcccgcgac gccttcctct
     3541 ccgtccacgg cttcgacggc gtgcccgaag gctacgaggt ccatcatgtc gtgccgcttt
     3601 ccgagggcgg cgccgacacg cccgataaca tgatcctcgt gagcgaggcc gaccatgcgg
     3661 cgatcacctc ggcgcacgcg ggcttctacg gctggcgaga ctgaggtacg ggagccgccg
     3721 aggcacccga aggcatgatc gaaaatatgc agacagtccc gcgaggcgaa gtgcctgcgg
     3781 ggctgtgcag gaagagagag gtgaggcggg cgttattgca ggtcgacgcc gaggaagtcg
     3841 agcgcggtct gaacgtgaag cgcggcgccc ttgacgagga cggactcgtc gaccttgtag
     3901 cagccgtggt gctgcggcca gacggcgtcg caggcctcgt tgcgcacgcc gagaaggacc
     3961 atggcgccgg gaaccttctc ctgatagaag gcgaagtcct cgccgcccat ctgcaggtcc
     4021 gcctcgatga cgcccttgtc gccgaagatt ttgcctgccg cgcccgtgac gacgtcgatc
     4081 atgcccggat cattgaccgt gacgggcacg atgcgcaggt attcgacatc ggccgtgccg
     4141 cgcatggctt cggccgtgct cctcgcgatg cgggtgatct gctcctcgaa gcggtcgcgc
     4201 acggcggggc tgaaggtgcg ggtcgtgccc tcgagttcgg cggtgcccgc aacgacgttg
     4261 aagtgggtgc ccgagtggaa cttgccgacc gtcacgacgg cggtgtcgcc gggataggtc
     4321 tcgcgggaga cgatcgtctg caggttctgc gcgatggcgg cgcccatgat gagcgcgtcg
     4381 gcgccgagct gcggctgggc gccgtgagtg gacttgccgc ggacgtggat cttgaactga
     4441 tcgcccgagg ccatcatcgg ccccttgcgc agcgcgaccg tgccggcggg atagtcggac
     4501 cagacgtgca tgccgaagca ggcgtcgacg ccctcgagcg cgccttcctc gatcatggcg
     4561 cgcgcgccca gacccagctc ctcggcgggc tggaaggcga agacgatcgt gcccttgaga
     4621 cggtccctga tttcgttgac gatcattgcg gccgtgagca gcatcgagat gtggcagtcg
     4681 tgcccgcagg cgtgcatgac gcccggaacc tcgctcgcgt agggaagtcc ggtctcctcg
     4741 ttgacggaga gcgcgtcgat gtcgccgcga agaaggatcg tgcggccggg ctgagcgccc
     4801 tcgatgcggg cgagcgtgcc cgtgccgagg ccgcagggac gccacgggat gcctgcgcgg
     4861 tcgagctcgg cgcggatgcg ttcggcagtg cggacttcct tcgtgctttc ttccgggtgc
     4921 tggtggaacc agcggcgcat ttcgatctgg tacccgttcc agcgggtcat gagttcctgg
     4981 agtgtcattt tttggtcctg tcaagggtct tcaggcgcag gtcccgtgcg gaagcgctgc
     5041 gcctgaatga aaagaggttt gaggccttcc gtcagaagag cttgacgaag aagcccgcga
     5101 gaatgacgga ggtgatcgtc accgtcacga agccgcccac gatcatcggc gggaagagtc
     5161 ggctcatgag gaagtcgacc tcggccttgt cggcgccttc ctcctggcag atgcgttcgg
     5221 tcatgatcgc gtcgaagggg aagccgtaga gagcggtgag gcaggttgcg aagccgagat
     5281 agggggagat cttgaggaag cgtccgacga tcgccgcgac gacggccatg ccgacgacgc
     5341 cgatgatgat caggaggacc atcgggcaga tgagcttcgc catcatttcg ggattgctgt
     5401 cgccgagacc gctgtagacg aacatgatca gagcgaggaa gaggaagtcc ttcgagccgg
     5461 tgcgcgagag cacgttgggc tcgaggaagc cgaggcggca gaagacgacg ccgcagacga
     5521 gcgcccagat ggcgccgttg accggcgtga gcacgccgag cacgaaggca agccaggcga
     5581 cgagcgagac cttgaggagc acgaagtacg gcgtgttcca ggccttgggc gtggcgagca
     5641 cgccctgctt ggattcgttg ccgggcatgg cgagtgcgtc ggccatgacc ttgcgggcgt
     5701 cctcgccctt gtcctcgacc ttgcccgcgc ggaaggcggc gagctggcgg cgggcctcaa
     5761 gcttgaggca gacggccgtg agcgggtagc ccgcgaagcc ctgcacgcag tacatgacga
     5821 tcgcgaagac ggctgcggcg gcaagaccgg cgtcaagcgc ggccttctgc atcatcgtgg
     5881 tggctacgat gccgcccgcg agcggcggga tgccggcgat gacgagcgtg cggtccatga
     5941 agagcggacc gacgaaccag cacaggccca cgagcccggc gaggcccgca atacagatga
     6001 ccacggtctt ccactgctgg agcaggcgct cgagactgaa gagcgtgccc atgtgggtga
     6061 tgaggaggaa gatggcgacg gaggagccca gcggcagaag cttcgagtcc gcaacgagcg
     6121 tctttgggag gaaggtccag aaggcgatga ggatcagcac ggcggagatg aagaccgacg
     6181 gaacccagcc tttcgtgaac ttggagagca cctcgcccag caggatgatg acgaggcagg
     6241 ccgcaagggc cgagacaaag ttgtacatga gcatggaatg gttccttgac gtgcggtagt
     6301 tggagtctga ggacgccgtc gcccggggtc gtcgacggcg cgtgcacgtt catgaattca
     6361 ttctgatccc gtccagaagc accggacgac cgaaggacgt cgaaagacga cgaaatttcg
     6421 acattccttg atgcgggaga ggggatttcg gcaaattgtc ttaagacaaa cgccttagag
     6481 gcccagatcg gagcctgatg atggaaacgc gtgcggtaca gtcgctttaa agcgacagca
     6541 ggcaaagacg gggagacgag cggatgaggg gggcggacca tcgggcgaga tggttctcat
     6601 gtacgtgaac gcaggcgtga gggcgggcag ggggccgccg atgttgagaa accgccgttt
     6661 cgctcaaccg gcaaaaacgc cgttttcagc ccgtttgttg cgtgaaagca acacctgttc
     6721 tccagagtcg gtgctgtaga gaacgccgat gcaggggctt ttcaatacgt cctgagacga
     6781 aagaaggacc ctttgttctg aaatgcgcgg acaggctcga aggagaaggc ggaaacggga
     6841 gaacgaatcg atgcggcccg catcgtcggt tcgtttccat ggcgaagcgt cgcggttcac
     6901 ggcgtcggat ccgctgcgtc aaacgtgggg caaggtttgg agaacgcttc gagggacgtc
     6961 ggtccgagcc gggggtgttc ttcagttatc gaacggggat gaaagtccgg aagggttgcg
     7021 gcagaaggaa gggcgcgagt ctgtgggtgg ggttgaagag aggtggcgca tcgttcgggg
     7081 attgcgcaca atggcgcaga gtcacggcgc ggtgctgtct gaagacgtcc tgaggggacg
     7141 tcgggagccg cgccctgatt gtctcttgtc aaccatcaaa ggaaagaaaa aatgaagaag
     7201 agttttgtcg ctctcgccgt tctcggcgct tttgccggca cttccatggc tgccgacgtc
     7261 acgatgtacg gtgtcgtcga tacgggcctt ctctatcagc acggtgaagc cagtgcgtcc
     7321 atcgacggca tcggcagcgc cagcgccgaa gtcgactcct tcagcatgga accgggcacg
     7381 aactccgcct cccgcttcgg catccgcggc acggaagacc tcggcaacgg catgacggtg
     7441 ggcttcaagc ttcgagtccg acacgggcgc cctcaagacg gacagcaagc tgtttgaccg
     7501 cgaatccacg ctcttcatca agggcgggtt cggtacagtc tacgccggcc gcatgggcac
     7561 gctcgtctcc gacgcgggct ccgttggctt ctacggctcc atggcctccg ctctggccac
     7621 gggctggtcc gacaacatcg ccggccacaa cgccgtcatg gccaactact cgagccgcta
     7681 tgacaacacg atcgcctacg tttcgccgga attcgccggc gtgacgatct atgcccagta
     7741 cgccatgggc gacacgaacg aaaacaaggc ttccaacgac cgctatgccg ctctcggtgc
     7801 tgaatgaaag gccggcgccc tcgacctcgc cgctctcgtc gactggacga acaaggactc
     7861 ctccatcacc cacagcaacg gcgccgaatt ccaggcggac atgaaccttc aggatgcctg
     7921 gacgttcaac ctcgccggtt cctatgactt cggcttcatg aagacctatc tcgccaccca
     7981 gtacttcaag gatgcccgtg acgcgggttc cgtccttgac tatgtgggct tcttcgacga
     8041 agacagcgtc ttcaacaata acgaaggcga cgtcaagttc aacaagggtg ccgttgccaa
     8101 caccggctat ggcgtgcatc tctccacggc gttcgacgtt ctcggcggca ctatgaaggc
     8161 cggcatcggc tacatggacg ccgacatcga ctacgccaac gacggccagg tcgccgactc
     8221 caaggtctac acggctgcct tcggctacga atactcgctc tccaagcgca cgctcgtcta
     8281 cacgggtctc ggctacacga agcgtgagct cgacgccaac tacgaaggcg tgaccggcag
     8341 ctgggaagaa gaaggctacg atttcgccct cggcctcgtc cacaagttct gagctgcctg
     8401 atcttcaggt ctgactgaac gcgcccgcag gaggattctt ctgcgggcgt tctgtttttt
     8461 cggtgcggga agacaagggc gtcatgccca ggcttcgagg aagcagtgct tgaggaccga
     8521 gaagtcgccg aagcgaacgc ccctcggggc ctccctcatg acgacgggct tgccgtggta
     8581 ggcgaagccg aagcccgcgg cggcgatcat ctgcaggtcg tttgcgccgt cgcctcccgc
     8641 gatcgtctcg gagagcgagg cgccgttgac ttgggcgagc acctcgagcg cacgccgctt
     8701 gccgtcggca tcaaggatct tcccgcccgc agggccgcgg acgccgcccg taagcctgcc
     8761 gtcctcaagg acgaggcggt tgcagaccac gcccgtcatg ccgaagcggc gggcgacgtg
     8821 cgtggcgatc tcctcgaagc cgcccgtgat gatgtaggtg gcgagaccgt gccgcgtgag
     8881 aaagtcgacg aagtcaaggg cgccgggcgt cggaacggcc cgctcgacgg cgtgggtcac
     8941 gatctcggcg ctcgatcccg cgagtgcggc cacgcgctgg cgcagcgact cgtcaaacgg
     9001 aatcagccct tccatcgccc ggcgggtgat tcgggagatc cgttcgcccg cacccgcgaa
     9061 ggcggccatc tcgtcgatgc actcgctctc aatgagcgtc gagtccatgt cgaagcaggc
     9121 gacgcgaaag cgcctgaggg aaagatcggg cggcaggagc aggacgtcga cggcgctctc
     9181 ttcggccatg gccgcaagcc ccgtcgggaa gcctttcgag ctcacgcccg aaagacggat
     9241 gaagccgggt ctgacgagct tcgtcctgaa gttgaggcgg cctgcaaatc cgaggatcgc
     9301 acggtcgtcg cctgcaagaa gcatgtcgtt catcgatttt cctctttcaa tcagtcatcg
     9361 tcgtccgtgc gggcgccttc gggaagaccg cgctcgggcg tccagccgtc gttgttgatg
     9421 cgatgggcgc aggtgacgta ggcgcggcct ccggatgaga gtcgttcaat tcgctggtgg
     9481 aaggcgccga aggtgaggtc gaaggcctcg cccgcaggcg tcctgcggac aggaccgccg
     9541 ggaagcgcct cgtcgagaag gcggcccgtg ggcttcacca tgtcggagcc ggagagaagc
     9601 ccttcgagcg tcatgatcgt gacggcgagg tcggcgtggc cagcaggatc gtcgtggacg
     9661 gccgagccgg ggaaggcgct tccgccgacc gcgagcacgg cgttgatctc ctgaggcagg
     9721 agtccgccgt ggtttcctgc gccaagaggc agctcgggcg ccatgacgag gccgcccgtt
     9781 tcgggatctc cgcgcgggac gatgcgaagg tcgggcgacc ggcggccgtc ggtgaagacg
     9841 aggcgctcgg agaaggtccc cgggattcgg ccctcgattg agcagggctg ctcgggatcc
     9901 gagggcgaga agaggagccc gatgtcgggc gaggtcatga gcgcgtcgcg ggcgcgaagc
     9961 tgaagcgaga ggtccgcggg attcgtgaac cagagtcccg cgcagtaggt tccgacgagg
    10021 acggcgtcgg ccgaggccgt gtcggcccct tcgagcacct cgcggcccat gaggatgctc
    10081 agacccgctt tttcaagaat gcccttcatg tcgaagtggc ggcgcacgac gccgtggccg
    10141 tgatcagaca tgacgacgag ctgcacgccc gcgtccctgc cttccgtctc ccaccactcg
    10201 agcacgcgcg cgaactggcg gtctgcgtcg cgccttgcct cgcgggtgcg ctcgtcgtca
    10261 atgccgaact catgcgagga gtggtcgggc tcgccgatcc agaggaccgt cacgtcaccg
    10321 aggcccctcg ggagctcgcg ctcgaagaag aggtcgacga cgagcttcgt gcccctgaag
    10381 tcgggaaatt caagcggaac gcccgggccg aaatcttcga cgagctcgcg gcgctcgttg
    10441 tccggaacgc tcgtcacgag gtcgtgcacg caggcgcaga cgtggtcggg ataacggtcg
    10501 gcgtagaggt gctgaaggcg cgtgctgccc ttcgagttgg cgcagtagac gcgcatggtt
    10561 ttgccggcgg cgccgagacg ctcgcccatg gtgggaacga gcaggagccc gtcctcgcgg
    10621 cgatggcgca gcacgtgctc gaggtcgaag ccgaagaaga cgccgtcggc gcctcccatg
    10681 tcgcggcgcc agtagcagtt ggcgacgagg ccgtggcccg tcgggcgaaa gcccgagaag
    10741 atgccgggat ggttgacgta ggtctcggtc ggaaagtccg caaggtagtg cgaccacgag
    10801 tggttcgagg ccatgaagcg cgagagcgcg ggcatgtcgg cctcgacgct gtcggggcga
    10861 aggccgtcga agcccacgat cacgaagcgg acggcggcgg atgaggagga atttgaggtc
    10921 atgagtgtct ccttatcgtg cggcgggttc gagcttctgc tggacccagt cgccgaggat
    10981 ggacatggag agcgtggaga cgacgatcac gaggccgggg atcacggcga tccaccaggc
    11041 ggtcgtgagg tagtcgcgcc cgaagcccag catggtcccg agggagctca tcggaggctg
    11101 aacgccgacg ccgaggaagg agagcgaggt ctcgaggagg atcatgccgg ggaagttgag
    11161 ggtcatgttg acgacgaggg ccgaggcgat gttgggcagg atgtgcctca gggcgatcga
    11221 gaccgtgggc agtccgaggc cctcgagtgc gcaggcgtag ccctcggtgt aggcggagcg
    11281 cgagaggttt cgcgtgagcc ttgcgtagcg gtcccatccg tagacggcca tgatcacgat
    11341 gatgacggtg acgtcggtgc cgaggaaggc gatgatcgtg agggccagga tgatgaaggg
    11401 gagcgaggcc gtgaagtcga tgccgcagtt gacggcgtcg tcgacgatgc cgccgaagcg
    11461 ggcggcgaga aagccgagca gcgtgccgat gacggcgccg agcagcgtac ccacgacggc
    11521 gagcacgaag gacatgcgga tcgagtagag aaggcgcgag aagatgtctc gcccgagctc
    11581 gtcggtgccg aggatgtgag cggcggactc aaacggcatg aggaggcggg ccgtgaggtc
    11641 catggcctcg aggtcgtacg gggcgaggac gggcgcgagg agccctgcgg tgaagaagac
    11701 ggcgaggatg atgcctgccg caatggcgag tgcgggacgt gtgttcatca gttgtctccc
    11761 ttgcgaaggc gcgggttgac gacgagtgcg agaaggtcca tgagaaggtt tgcggtgacg
    11821 agcgaggcgc ccgtgaccaa cacgatcatc tgcacgacgg gaatgtcgcg cgaggcgacc
    11881 gactggacga ggagcttgcc gacgccgggc caggagaaga cgttttcggt gatgacggcg
    11941 cccgcgacga gcgtgccgag gaagaagccg aggatcgtga ggatcgagag catggcgttg
    12001 ggaagcgcat ggagccagat gatcctgcgc tcgggcaggc cgcgcatgcg ggccgccctc
    12061 acgcacggga ggcgcagcgc gtcgatcatg gcgcttcggg cgtagcgcga gaagatcgcg
    12121 gcctccgagg tcgccatcgt gatgatcggc atgatgtagt gccagacggt tgcgttgccg
    12181 taggacggga gccatccgag ctggatcgag aagacgagca gaagaaggac gcccatgaag
    12241 aagttgggca ccgcatagcc gaagaccgac atcaccatga ggctgcggtc gaggagcgtg
    12301 ttgcggtgaa gcgcggcgca cacgcccgac ggaatgccga tgaggagcgt cacgacggcg
    12361 gtcggaatca tgagcgagag cgtgggcccg agcgcgtcga ggaagacgtt cctcacgggc
    12421 tggcccgtca tgtagctcgt gcccatgtcg agcgagacga acttctcgag gtagacgagg
    12481 aactgctgcc agagcggcag gttgaggccc cactgggcgc ggaaggcctc gagcgccacc
    12541 tcgtcggcgg cgttgccgag catgatgcgg gccggatcgc ccgtcatgtg aaggccgaag
    12601 aagacgcagg ccgtgaggag caggagcgtg aacgcggcgc gaagaaggat tcggaagacg
    12661 acggcgaaca tggatcaggc ctccggatgc aagaggaagg aggggcccga aaagcaggcc
    12721 gagaggtgtc cgctgccgtc gtccctgagg ggcggcacgt cggtgcggca gcgcgcctcg
    12781 gcaagcggac agcgggaggc gaagacgcag cccttcggac ggtcgatcgg actgggcatg
    12841 gctcctttcg ggtagtagcg cacgacgtgc gcgtcggcgc ccggagtcga ggccatgagg
    12901 agcttcgtgt agggatgcag gggccggctg aagaccgctt cgacggtgcc cgactcgacg
    12961 atgcggccga ggtacatcac ggtcacgcgg tcgcagatgc agcgcacgac gctgaggttg
    13021 tgcgagacga agagcattgc catcttcatg cggttgcgaa gctgcaggag gagttcgagc
    13081 acctgcgcct gaatcgagac gtcgagcgag gcgacgggtt cgtcgcagac gagaagcgcg
    13141 ggttcgagcg ccacggcgcg ggcgatggcc gcgcgctgga gctggccgcc cgacatgacg
    13201 gacggacgcc tgccggcgtg gtgcgcggga aggcctgcgg ccctgagcac ttcgagcgcg
    13261 gccttcgcgg ccgcctcctt cgagaggccc ctgtggtggc gaagcggttc ggccacctgc
    13321 tcgatgatcg gaagacgcgg atcgaagcag gcgtatgggt cctgatggat catctgcatg
    13381 gaggcgcgct gggcgcgcca ggcggcggag tctcgcccgg ggatcggcgc gttcttgaag
    13441 cgcactgtgc cttcggtggg ctcgatgagg ccgagcgcga ggcgcgcgag cgtcgatttg
    13501 ccgcagcccg attcgcccac
//
LOCUS       DBMDTI010000032        13463 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1403_length_13463_cov_2.759093, whole genome shotgun sequence.
ACCESSION   DBMDTI010000032 DBMDTI010000000
VERSION     DBMDTI010000032.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 13463)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13463)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13463
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1403_length_13463_cov_2.759093"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            3..2447
                     /gene="lon"
                     /locus_tag="ACZPB6_03015"
     CDS             3..2447
                     /gene="lon"
                     /locus_tag="ACZPB6_03015"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR1183482.1"
                     /translation="MPAVAVRDMVIFPSTLVPVFMAREASVNAARKAFQSDNKYVFVV
                     TQKEPDTDAPKQDDCYEVGTLASVMQFFGLADGTSKMLVEGLARYRVDSLFERDGSLV
                     ARCTRLHTVYAPERTLEPLRRQLMSEWKKYGSFGTGKSEPAGRFDAETDPERLLDLVC
                     GQMSGLAAAKMEMLAEVDLAARFRHGIDFLLTQIDLQKLNVRIDGRVKRQMDKNQRDY
                     YLHEQLKAIRKELGDTDEGADEEYADLEARIASAKMPKYAEETVRNELRRLRQMPAMS
                     SESVVVRSYIETLTDYPWTKKSRVSRDLEAAARILDEDHSGLEKVKERILEYLAVQKR
                     VGKVKSPILCFVGAPGVGKTSLGESIARATGRKYVRVALGGVHDESEIRGHRRTYVGS
                     MPGQIIKSMIRAGVRNPLFLLDEIDKMGTDHRGDPASAMLEVLDPEQNNAFADHYMEI
                     PVDLSDVMFVATSNSYDIPPALLDRMEVISLSGYTEEEKIHISEHHLIPKQLRATGLK
                     AEEVDLPREAIVDIIRYWTREAGVRGLERLIGKIFRKVVFAADKKGGSMPEKVVIKPE
                     SLSTYLGPAKYTIGLASKEPRVGVVNGLAWTSVGGEMLVIEAQIFPGKGGVQRTGSLG
                     DVMKESVETARSVVRARAANLGIDTKRFYETDLHVHYPEGATPKDGPSAGIATTTAII
                     SAMTGIPVRSDVAMTGEITLHGEVLEIGGLKEKLLAAVRAGCTKILIPDVNRRDLEDV
                     PETAKTQIEVIPVKTIDEVIAQALVRLPEPLAVEAETAGAAAGAATGEAAASAAEERS
                     SKASSAGKTPTLEVHA"
     gene            2657..2732
                     /locus_tag="ACZPB6_03020"
     tRNA            2657..2732
                     /locus_tag="ACZPB6_03020"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2690..2692,aa:Val,seq:tac)
     gene            2831..2907
                     /locus_tag="ACZPB6_03025"
     tRNA            2831..2907
                     /locus_tag="ACZPB6_03025"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2865..2867,aa:Asp,seq:gtc)
     gene            2955..3031
                     /locus_tag="ACZPB6_03030"
     tRNA            2955..3031
                     /locus_tag="ACZPB6_03030"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2989..2991,aa:Asp,seq:gtc)
     gene            3158..3234
                     /locus_tag="ACZPB6_03035"
     tRNA            3158..3234
                     /locus_tag="ACZPB6_03035"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3192..3194,aa:Asp,seq:gtc)
     gene            3356..5263
                     /locus_tag="ACZPB6_03040"
     CDS             3356..5263
                     /locus_tag="ACZPB6_03040"
                     /inference="COORDINATES: protein motif:HMM:NF025010.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR1183483.1"
                     /translation="MLQAFRDHKRWLMFIAMVLIIPSFVVTGIYSYNRMTQADNSIAK
                     VGDSSITPELFDQAKRQQLERLRQQMGDQFRAGMLDTPEAREALVRNLMDEAAVTQTV
                     AREHVGVSEQEAVALIKNADALKENGQFSPALYERFLQSQGKSDQQFVAEVRRDLAKE
                     ALLTGVTATYPVPSAVVEHLHKIITEEREVQTLIFNIDQYMGDVKVTPDEVKAYYDGH
                     QSEFMADEHVTAQYVVLSPESFKASIKPNEEDVRSYYEQNKQRYATAEERRASHILIS
                     FGDDKAASKKKAEEILAKAKADPSSFAKLAKENSVDTGSAVEGGDLSFFGRGMMVKPF
                     EDAVFSAKKGDIVGPVESDFGWHVIYVTDIHPARVRDFNEVRADIEAEYIDQMALREF
                     SEKAEEFTNIVYEQADSLEPAAQKFGLKLMTAEDVTRQGPADPELAPFFNEHVLENLY
                     GSECLQEKRNSAAVEVGSNQLVAARVEKHFPKAVRPFDEVKGQIEDGLKLRKAGELAK
                     AAGEKKVAEVRQSKSLDGFSKAVWVSRQNLLGHPAQLVDRMVALPADKLPAYTGMQVD
                     GGAYFVAFVNAAKVKAPTENELKSLSREFATIYGEADRRGYLSALRQELGEEILRPDF
                     IKGENKGEDAE"
     gene            complement(5403..6953)
                     /locus_tag="ACZPB6_03045"
     CDS             complement(5403..6953)
                     /locus_tag="ACZPB6_03045"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR1183484.1"
                     /translation="MSKLYMGFDAGTQSVKVAVYDEQFNCLTSHSLPTHLHYPQPGWV
                     EMSADEFLEITVKSMAACAQSLREMGRDPKDVCAVMGDGIICGIVGVNPDGDAVTPYI
                     NYLDSRTTPDVEALNARELEIWGRETGNPVASPMFPAMFARWFLKNNEAFNRDGVKFV
                     HNAPYILMHLAGLKGEDAFIDWGAMSGWGLGYDVQKKCWSDEQLEILGIDRKYMPEIV
                     KPWDKVGGLSEAMAERTGLRAGIPILGGAGDTMQSMLGSGIFEAGQGVDVAGTCAMFC
                     VSTKGIIPELSKPGTGLIFNSGTLPDTYFYWGFVRTGGLALRWFKDNVCKKADDAAYY
                     GPMTEGAEKVPAGCRGVMFLPYLTGGSGEEKEASGCFLNMTMDDDQFVMWRAVLEAIG
                     YDYMGLADSYRAAGVDLNRITVTEGGSRNELWNRIKSHMLGADVVRFRNAGGAVLTNC
                     IVAAHAVGDAPDVRKVLSDNIERDAEYAPVPALTSRYRTLYDMRQKLVREDMKTAFSR
                     LVAMRTIE"
     gene            7156..9135
                     /locus_tag="ACZPB6_03050"
     CDS             7156..9135
                     /locus_tag="ACZPB6_03050"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HIR1183485.1"
                     /translation="MAKTNGRAEDYGVSSIKVLKGLEPVKQRPGMYTLTDNPLHIIQE
                     VVDNATDEVLAGFGSRISVTLHADGGITVEDNGRGIPTGLHPEEKVPVVELVFTRLHA
                     GGKFAKSDGSGPYSFAGGLHGVGVSVTNALSTTLDVTVWRDGEESTIGFADGDVVSPL
                     KSRRSPRPKSQTGTRVTAHPDPKYFDSPAIPEGALIRLLRSKAVLLPGCEVVYKNEKT
                     GREECWKYEGGLREYLLSEIAAEPLVAPFEAHGYVEDAGDGYAVGEGAAWVVIWTTEG
                     GLERESYVNLIPTPQGGTHEAGLKDGLYQAMLAFMQAHGLQAKNVKILSEDVFSRANF
                     VLSTKALDPQFQGQTKEKLTSRETMKLVSGFVRPQFEFWLNDHIEEGKKLAELIVSQA
                     QARQRQSTKYEKKKSSSVAVLPGKLTDCESDDISMNELFIVEGDSAGGSAKSGRNKEF
                     QAVLPLRGKILNTWEVDADKIFGSDTVHDIAVAIGVDPHPADGAPDLKGLRYGKICIL
                     SDADVDGSHIQVLLLTLFYRHFPKLIELGHVYVAMPPLYRVDVPKRGRKPERKFYCLD
                     DRELSRTLTALRKEGFEEDKISISRFKGLGEMKAQQLAETTLHPDTRRLLPVGMGDVD
                     EAVTGEVMNLLMATKSAGSRKLWMEEYGDRVEADV"
     gene            9152..11905
                     /gene="parC"
                     /locus_tag="ACZPB6_03055"
     CDS             9152..11905
                     /gene="parC"
                     /locus_tag="ACZPB6_03055"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIR1183486.1"
                     /translation="MARKKGGLNPEVQDDGGLFGDQMLVYEDDLKKAEETPARKKSAG
                     GRVAKASAEAPVQTPLLPEVEESVEVQADEPVQETAPEESSAREIEEDVTEEARIEED
                     AAEASEDEGASPLGARVDEVMTGGVESDGQLTLARYASRAYLEYAMSVVKSRALPDVS
                     DGQKPVQRRILIDMERMGVKAGTKAVKSARVVGDVLGKYHPHGDQSVYDALVRMAQSF
                     SLRYPLIDGEGNFGSRDGDSQAAMRYTEARLMPIASLLLEEVDTGAIDFTPNYDGNFQ
                     EPVVLPAKLPFVLLNGSSGIAVGMATDIPSHNLREVAAACTLLIDKPEATLDEVLEVL
                     PAPDFPCGGQIISPRSTIRDIYRTGRGKLTVRARYHFEDLARGQWQLVIDELPPASSS
                     QDVLNQIEAITNPKARKDKKTVSSKQQQAKSAMLAILDRVRDESTEEKPVRLVFEPKS
                     SRIDREEFVAALLSQTSMESNVSVNLVMLGNDGKPKQKNLLEIIREWIAFRLLAVRRR
                     TQARLDKVNDRIHVLEGRALVLLNIDRVIEIIRNSDEPKPALIKEFGLTDRQADDILE
                     IRLRQLARLAAIAIEKELAELNKEKSSLERILGSDGVLRRTVAREIEQAAKTFGDDRR
                     TLIEEASVVQAEQHVVDEPVTVVLSQKGFLRTRQGHGHDCTLLSYKVGDGFECAVECR
                     TVDDISFVTDTGRVYTCKVSAIPGGRGDGLPVTSFFDLDKGMKRFVGVFAGREKEDVV
                     LGTSDGLGLKCTIGDLKASQRIGRTFVRMADGVTLMPPAEGCAEMPLIACLTEKGRLV
                     IFAHDEIRRLSNGGMGVSLVKLESGERMIGMTLVDERGLTASGTGRGGKPREVQITRK
                     SFEEFRMQRARKGRQIGVTWTVESIAPITPVKPENEGEGSSGAGSAGGLTIQLEDENP
                     RLI"
     gene            11938..>13463
                     /locus_tag="ACZPB6_03060"
     CDS             11938..>13463
                     /locus_tag="ACZPB6_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR1183487.1"
                     /translation="MKLNPLRFFKSLSARLLLLTLIWVSFIVTTIGYTMMLNWKLESS
                     SAATNIIGDIRFHVFRTALYVLPQYDNRDFDNEVRTVNAGLDLLQKGDQWRPLLVPET
                     QAIRSSLQSIDSEWKESVLPHLTAARGGAREPMMGDVNLYVEKLAALTNDIDEYRAHF
                     LWQLRYLQGLLIVLAIGSLFAIMALLLRWVIRPLEQLGGAIGRLSSGDLTARTEVRSE
                     DEIGEIAAGFNEMAARLKDSHDNLEQKVAEKTASVEEKNSHLAQLYEMTSYFTQRRSL
                     DDLADGFVSRIMRQTEADACTLQLLSGRDDSMQLLTAEGLSVDLVKAVNDLPGDAGIV
                     PSVLSKTYPVCFQLTELEDEFSRRFADAGFKTAYSFQIRSTPGDLGIFTLFFKESPQL
                     TTQVIRLLENFVTHFGVAVASNRLIERDRQFAVVQERQLLAQGLHDSIAQALSFLNLQ
                     VQFLSDAIRTNDVALRDESLSAICTGVQECYEDVRELLLNFRERLHKEGFIEGVRTVI
                     "
BASE COUNT         2674 a         4141 c         4143 g         2505 t
ORIGIN      
        1 aagtgcctgc tgtcgcagtg cgggacatgg tgatcttccc ctcgacgctg gtgcccgtct
       61 tcatggcgcg cgaggcttcc gtcaacgccg ctaggaaggc ctttcaatcc gacaataagt
      121 acgttttcgt ggtcacgcag aaggagcccg acaccgatgc gcccaagcag gacgactgct
      181 acgaggtcgg cacgctcgca agcgtgatgc agttcttcgg cctggctgac ggcacctcga
      241 agatgctcgt cgagggcctc gcccgctatc gcgtcgactc gctcttcgag cgtgacggct
      301 cgcttgtggc tcgctgcacc cgcctccata ccgtctacgc cccagagcgc acgcttgagc
      361 cgctgcgccg ccagttgatg agtgagtgga agaaatacgg ctccttcggc accggaaaga
      421 gcgagcctgc gggccgtttc gacgcggaga ctgatcccga gcgtctgctt gaccttgtct
      481 gtggccagat gtccggcctt gctgccgcca agatggagat gcttgccgag gtcgaccttg
      541 cggcgcgctt ccgtcatggg atcgacttcc tgctgaccca gattgatctt cagaagctca
      601 atgttcgcat cgacggccgc gtcaagcgcc agatggacaa gaaccagcgc gactactatc
      661 tccacgagca actcaaggcc atccgcaagg agctcggcga cacggatgag ggcgctgatg
      721 aagaatacgc cgatcttgaa gcccgcatcg cctcggcgaa gatgcccaag tacgccgagg
      781 agaccgtgcg caacgagctt cgccgcctgc gccagatgcc ggcgatgagc tccgagtccg
      841 tcgtcgtgcg cagctacatc gaaacgctca cggactatcc gtggacgaag aagtcccgcg
      901 tttcgcgcga ccttgaggcc gccgcccgca ttcttgacga ggatcactcc ggtctcgaaa
      961 aggtgaagga gaggattctc gagtatctcg ccgttcagaa gcgcgtgggc aaggtgaagt
     1021 ctccgattct ctgcttcgtg ggtgcgcccg gcgtcggcaa gacctcgctc ggcgagtcca
     1081 tcgcccgagc cacgggccgc aagtatgtgc gcgtggctct gggcggcgtg catgacgaaa
     1141 gcgagattcg cggccaccgt cgcacctacg tgggctcgat gccaggtcag atcatcaaga
     1201 gcatgattcg tgccggcgtg cgcaacccgc tcttcctgct tgacgaaatc gacaagatgg
     1261 gcacggatca tcgcggggat cccgcttcgg ccatgctcga ggtgcttgat cccgaacaga
     1321 acaacgcgtt tgccgaccac tacatggaga ttccggtgga tctctccgac gtgatgtttg
     1381 tggcgacctc gaacagctac gacattccgc cggccctgct ggaccgcatg gaggtgattt
     1441 ctctttcggg ctataccgag gaggagaaga tccacatctc cgagcaccac ctgattccca
     1501 agcagcttcg cgccacgggg ctcaaggctg aagaggtcga cctcccccga gaggccatcg
     1561 tcgacatcat ccgctactgg acccgcgagg cgggcgtgcg cggacttgag cgtctcatcg
     1621 gcaagatctt ccgcaaggtt gtgttcgccg ccgacaagaa gggcggcagc atgcctgaaa
     1681 aggtggtgat caagcccgaa agcctcagca cctatcttgg gcccgccaag tacacgatcg
     1741 gccttgccag caaggagccg cgcgtgggcg tcgtcaacgg cctggcctgg acgtccgtgg
     1801 gtggtgaaat gctcgttatc gaagctcaga tcttccccgg caaggggggt gtgcagcgta
     1861 cgggcagtct cggcgacgtg atgaaggaaa gcgtcgagac ggcccgctcc gtcgtgcgcg
     1921 cccgtgcggc caacctcggc atcgacacga agcgcttcta cgaaaccgac cttcacgtcc
     1981 actatcctga gggtgcgacg cccaaggacg gtccgtcggc aggcatcgcc acgaccacgg
     2041 ccatcatctc ggccatgacg ggaattcccg tacgctccga cgtcgccatg acgggcgaga
     2101 tcacgcttca cggcgaagtg ctcgaaatcg gcggcctcaa ggaaaagctc ctcgccgccg
     2161 tccgtgcggg ctgcacgaag attctgatcc ccgacgtcaa caggcgcgat cttgaggacg
     2221 tgcccgagac cgccaagact cagatcgagg tcatccccgt gaagaccatc gatgaggtga
     2281 tcgcacaggc gctcgtccgc ctgcccgagc ctctcgcggt cgaagctgaa acggctggag
     2341 ctgcagcagg ggccgcgacc ggcgaagccg ctgcgtccgc cgcagaggaa aggtcctcca
     2401 aggccagcag tgcgggcaag actcccacgc ttgaggtcca tgcctgacct tgcgggaaga
     2461 gggccttctg agaaggaggt tcctcgcatg aaatgatgtt cggcccgtcc gcgcaagcgg
     2521 tcgggccgaa cacgtttgag gaatggccga agcgagcaag gcataccgtg tttgcggtat
     2581 ggttttgcct agggcatcgt gtacaatccg agttctgcag cgcgcaacga ggcgtcatgc
     2641 aggactttta ccgtgggggt gcttagctca gttggtagag caccgccttt acacggcgat
     2701 ggtcgggagt tcgaccctct cagcacccac cagtcaaaaa agttgaggag ttttggaaaa
     2761 aaaaacttct tgacagattg gaaaagttct gtataatacg cttctctgct gatgcaaaag
     2821 ctctgaaaac ggagtggtag ttcagttggt tagaataccg gcctgtcacg ccgggggtcg
     2881 cgggttcgag tcccgtccac tccgccaacg tttccgagcg tacatcgaca gattgcgaca
     2941 agcacatgat gtgcggagtg gtagttcagt tggttagaat accggcctgt cacgccgggg
     3001 gtcgcgggtt cgagtcccgt ccactccgcc aacagatttt aagaagaatt cgatacaaaa
     3061 gtaccgggtt tttcaaaaaa ggcgaaaacc gaaaaagcag aagttcgcta aaatatcgga
     3121 cttaggtttg agccgcgtgc gaaagcacgg catgtgcgga gtggtagttc agttggttag
     3181 aataccggcc tgtcacgccg ggggtcgcgg gttcgagtcc cgtccactcc gccaacagat
     3241 tctgaaaaga ggcgaacctt gcggttcgcc ttttttttgc tctaaaaaat catcgggaac
     3301 gaggcctctc tgcgtccgtc gggcgacagt tggccaagac aacaaggaaa cgttcatgct
     3361 tcaggcattt cgcgatcaca agcgctggct gatgttcatc gccatggtgc tgattattcc
     3421 gagcttcgtc gtgacgggca tctacagcta caaccgcatg acccaggctg acaactccat
     3481 cgccaaggtc ggcgacagct ccattacgcc ggaactcttc gaccaggcta agcgccagca
     3541 gctcgagcgc cttcgccagc agatgggcga ccagttccgt gcaggcatgc tcgacacgcc
     3601 tgaggcccgc gaagccctcg tgcgcaatct gatggacgag gccgccgtca cccagacggt
     3661 tgccagagag catgtcggcg tgagcgagca ggaagccgtt gcgctcatca agaacgccga
     3721 tgcgctcaag gagaacggtc agttttctcc cgcgctctac gagcgcttcc tccagagcca
     3781 gggcaagagc gaccagcagt ttgtggctga agtccgccgc gacctcgcca aggaggctct
     3841 tctcacgggc gtgaccgcca cctatcccgt gccgtccgcc gtcgttgagc atctgcacaa
     3901 gatcatcacc gaagagcgtg aagtccagac gctcatcttc aacatcgacc agtacatggg
     3961 cgacgtgaag gtgacgcccg atgaggtcaa ggcctactac gacggccatc agtccgagtt
     4021 catggccgac gagcacgtca cggcccagta cgtcgtcctc tcgcccgaaa gcttcaaggc
     4081 ctccatcaag cccaatgaag aggacgttcg cagctattac gaacagaaca agcagcgcta
     4141 tgccacggct gaggagcgtc gtgccagcca catcctgatc agcttcggcg acgacaaggc
     4201 cgcttccaag aagaaggccg aagaaatcct cgccaaggcc aaggccgatc cgtcctcctt
     4261 cgccaaactg gccaaggaaa attccgtcga cacgggctcc gccgttgagg gcggcgatct
     4321 ctccttcttc ggccgcggca tgatggtcaa gccctttgag gacgccgtct tctccgccaa
     4381 gaagggtgac atcgtcggcc ccgtcgagag cgacttcggc tggcacgtca tctacgtgac
     4441 cgacatccat ccggcccgcg tgcgtgactt caacgaagtc cgcgccgaca tcgaggccga
     4501 atacatcgac cagatggctc tgcgcgaatt ctccgaaaag gcagaagagt tcacgaacat
     4561 cgtctacgag caggccgaca gcctcgagcc cgccgcccag aagttcggcc tcaagctcat
     4621 gaccgccgag gacgttacgc gtcagggtcc tgccgatccc gaactggctc cgttcttcaa
     4681 cgaacacgtg cttgaaaacc tctacggctc cgaatgcctg caggaaaagc gcaattccgc
     4741 tgccgttgaa gtgggctcca atcagcttgt cgccgcccgc gtcgagaagc acttcccgaa
     4801 ggccgttcgt ccgtttgacg aggtcaaggg tcagatcgag gacggtctga agctgcgcaa
     4861 ggcgggtgag ctcgccaagg ccgccggcga gaagaaggtc gccgaggtcc gccagtcgaa
     4921 gtctcttgac ggcttcagca aggccgtctg ggtctcccgc cagaatctgc tcggtcatcc
     4981 cgcccagctc gttgaccgca tggttgcgct tcccgccgac aagctccccg cctacacggg
     5041 catgcaggtt gacggcggcg cctacttcgt cgcgttcgtg aacgccgcca aggtgaaggc
     5101 tccgaccgaa aacgagctga agtccctctc gcgcgagttc gccacgatct acggcgaagc
     5161 cgatcgccgc ggttatctct ccgccctccg ccaggagctc ggcgaagaga tcctccgccc
     5221 cgacttcatc aagggcgaga acaagggtga ggacgccgag taattccggc tttgcctgat
     5281 cccgcaaagg ggcgcatgtc cgacggacgg cgcccctttc ccattcttca aaagaagagg
     5341 gcgcccctcc tgaccggaaa gggcgcccgt tctcgagctg aacgggatcg aggcgttatt
     5401 tgttattcga tggttcgcat ggcgacgagg cggctgaagg cggtcttcat gtcctctcgc
     5461 acgagcttct gacgcatgtc gtaaagcgtg cggtagcggc tcgtgagcgc gggaacgggt
     5521 gcatactcgg cgtcgcgctc gatgttgtca gagagcacct tcctgacgtc gggcgcgtcg
     5581 cccacggcgt gggccgcgac gatgcagttc gtgaggacag caccgcccgc attgcgaaag
     5641 cgcacgacgt ccgcgccgag catgtggctc ttgatcctgt tccagagctc gttgcgcgaa
     5701 ccgccttccg tcacggtgat gcggttgagg tcgacaccag ccgcgcgata gctgtcggca
     5761 aggcccatgt agtcgtagcc gatggcttca agcacggcac gccacatgac gaactggtcg
     5821 tcgtccatgg tcatgttgag gaagcatccg ctcgcttcct tctcttcgcc cgaaccgccc
     5881 gtaaggtagg gcaggaacat cacgccacgg caacctgcgg gcactttctc cgcgccttcc
     5941 gtcatcgggc cgtagtaggc agcatcgtcc gccttcttgc agacgttgtc cttgaaccag
     6001 cgcagggcga gaccgcccgt gcggacgaag ccccagtaga agtaggtgtc ggggagcgtg
     6061 cccgaattaa agatgagccc tgtgcccggt ttggagagct cggggatgat gcccttcgtc
     6121 gagacgcaga acatagcgca ggtgcctgcc acgtcgacgc cctggcctgc ctcgaagatg
     6181 ccggagccaa gcatggactg catcgtgtcg cctgcgccgc ccagaatggg aatgcccgcc
     6241 ctgaggcccg tgcgttcggc cattgcctcg gaaaggccgc cgactttgtc ccagggcttc
     6301 acgatttcgg gcatgtactt cctgtcgatg ccgagaattt cgagctgttc gtcggaccag
     6361 cactttttct ggacgtcgta gcccaggccc cagccggaca tggcgcccca gtcgatgaag
     6421 gcgtcctcgc ccttgaggcc cgcgagatgc atgagaatgt agggggcgtt gtgcacgaac
     6481 ttcacgccgt cgcgattgaa ggcctcattg tttttcagga accagcgggc gaacatcgcg
     6541 gggaacatcg gggaggcaac ggggttgccc gtctcgcggc cccagatctc gagttctcgg
     6601 gcgttgaggg cctcgacgtc gggtgttgtg cggctgtcaa gatagttgat gtagggcgtc
     6661 acggcatcgc catcggggtt gacgccgacg atgccgcaga tgatgccgtc gcccatgacg
     6721 gcgcagacgt ccttcggatc cctgcccatt tccctgaggg actgagcaca ggcggccatt
     6781 gactttacgg tgatctcgag gaactcgtcg gcgctcatct cgacccagcc gggctgcgga
     6841 tagtgcaggt gcgtgggcag gctgtggctc gtgaggcagt tgaactgctc gtcatagacg
     6901 gcgaccttga cgctttgggt gcctgcgtca aagcccatgt aaagtttgct cattagggtg
     6961 gccttttctt gtaagggtcg atcggttaat tttatgcgaa gccacctgcc tgcgacgcct
     7021 tttcatcaga cggaggggag gggacggcct ttttgaaaac atgggttaca atccttgcac
     7081 agttttgagg cggatgcaga acggatccgc ctctttgttg gatgtgttga aaatcggtcg
     7141 ggtgaatcag taatcatggc gaagacaaac ggcagagcgg aggactacgg cgtctcctca
     7201 atcaaggtgc tcaaggggct tgaacccgtc aagcagcgcc cggggatgta cacgcttacg
     7261 gacaatcctc tccacatcat tcaggaagta gttgacaatg cgacggacga ggtccttgca
     7321 ggcttcggat cacgcatatc ggtgacgctt catgccgacg ggggcatcac ggtcgaggac
     7381 aacggccgag gcatcccgac ggggcttcat cctgaggaga aggtgcctgt tgtcgagctt
     7441 gtcttcacga ggctgcacgc aggcggcaag ttcgccaagt ccgacggctc gggtccgtat
     7501 tcctttgcgg gcggtctgca cggcgtcggc gtctcggtga cgaacgcact ttcgactacg
     7561 ctcgacgtca ccgtctggcg cgacggcgag gagtcgacga tcggctttgc cgacggcgat
     7621 gtcgtcagcc ccctcaagtc ccgccgttct ccgcgtccga agtcccagac gggcacgcgt
     7681 gtgactgctc atcctgatcc gaagtacttc gacagccccg cgattcccga gggtgcgctc
     7741 attcgcctgc ttcgctctaa ggccgtgctg cttcccggct gcgaagtggt ctacaagaat
     7801 gagaagactg gcagggagga atgctggaag tatgagggcg gccttcgcga atacctcctc
     7861 tccgagattg cagctgaacc gcttgtcgct cccttcgagg cgcatggtta cgttgaggat
     7921 gccggtgacg gctatgcagt gggcgagggc gcggcctggg tcgtcatctg gacgaccgag
     7981 ggagggcttg agcgcgagag ctatgtgaac ctcatcccga cgccgcaggg cggcacgcat
     8041 gaggcggggc tcaaggacgg cctgtatcag gccatgctcg ccttcatgca ggcgcacggc
     8101 ctgcaggcga agaacgtgaa gatcctctcc gaggacgtct tcagccgggc caacttcgtg
     8161 ctgtccacga aggcgcttga tcctcagttc caagggcaga caaaggagaa gctcacgagt
     8221 cgcgagacga tgaagctcgt gagcggcttc gtgcgtccgc agttcgagtt ctggctcaac
     8281 gaccacatcg aggaaggcaa gaagctcgcg gagctcatcg tctctcaggc acaggcgcgc
     8341 cagcgccagt cgacgaagta cgagaagaag aagtcctcga gcgttgcggt gctccccggc
     8401 aagctcacgg actgcgagtc cgacgacatt tccatgaatg agctcttcat cgtggagggc
     8461 gactctgcag gcggctccgc caagtcgggt cgcaacaagg agtttcaggc cgtgctgccg
     8521 ctgcgaggca agatcctcaa cacgtgggag gtcgacgccg acaagatctt cggcagcgac
     8581 accgtgcacg acatcgccgt ggcgatcggc gttgatccgc accctgcgga cggagccccc
     8641 gacctcaagg gactgcgcta cggcaagatc tgcattttgt ccgatgccga cgtggacggc
     8701 agtcacattc aggtgctgct tctcacgctc ttttaccgtc acttcccgaa gcttatcgaa
     8761 ctcgggcatg tgtacgtggc catgccgccc ctctatcgcg tcgacgtgcc gaagaggggc
     8821 cgcaagcccg agcgcaagtt ctactgtctc gatgaccgag agctctcccg caccctgacg
     8881 gccctcagga aggagggatt tgaggaagac aagatctcga tctcccgatt caagggtctg
     8941 ggcgaaatga aggctcagca gctcgctgag actacgcttc atcccgacac ccgcaggctg
     9001 ctgcccgtgg ggatgggcga cgtcgacgag gccgtgacgg gtgaggtgat gaacctcctg
     9061 atggcgacga agagcgcggg cagccgcaag ctctggatgg aagaatacgg cgaccgcgtc
     9121 gaggcggacg tctgagctgg agagagacga tatggcaaga aagaaaggcg gcctcaatcc
     9181 cgaggtccag gacgacggcg gtctcttcgg cgaccagatg ctcgtttatg aagacgattt
     9241 gaagaaggcg gaagaaacgc cggcaaggaa aaaatccgct ggcggcaggg tggcaaaggc
     9301 atctgccgaa gcacccgttc agacgcctct gcttcccgag gttgaggaga gcgtcgaggt
     9361 gcaggctgac gaacccgttc aggagacggc gcctgaggag tcctccgctc gcgagatcga
     9421 agaggacgtg acggaggagg ctcgcattga ggaggatgct gcagaggctt ccgaagatga
     9481 gggcgcgtcg cctcttggtg cacgcgttga cgaggtgatg acgggcggtg tcgaatccga
     9541 cggtcagctc acgctcgcgc gctatgcaag ccgggcctat cttgaatacg cgatgtcggt
     9601 cgtcaagagc cgtgcgcttc ccgacgtctc cgacggccag aagcccgtgc agcgccgcat
     9661 tctgatcgac atggagcgca tgggcgtcaa ggcgggcacg aaggccgtca agtctgcgcg
     9721 cgtcgtgggc gacgtgctcg gcaagtacca tccccacggc gaccagtcgg tctacgatgc
     9781 gctcgtgcgc atggcgcaga gcttctcgct ccgctatccg ctcatcgacg gcgagggcaa
     9841 cttcggcagc agggacggcg actctcaggc tgccatgcgt tacaccgaag cgcgcctcat
     9901 gccgatcgca agtctgcttc ttgaagaggt ggacacgggg gcaatcgact tcacgcccaa
     9961 ctatgacggc aacttccagg agcccgtggt tctgcccgcg aagctgccct tcgtgctgct
    10021 caacggctcg tccggcattg ccgtcggcat ggcaaccgac attccctcgc acaacctgcg
    10081 ggaggtcgcg gccgcctgca cgctgctgat tgacaagccc gaggcaacgc ttgacgaggt
    10141 gcttgaggtt ctgcctgcgc cggatttccc gtgcggcggc cagatcatca gcccgagaag
    10201 cacaattcgc gacatatacc gtacgggcag gggcaagctc accgtgcgtg cgcgctacca
    10261 cttcgaggat cttgcccgcg gccagtggca gctcgtgatc gacgaactcc cgcccgcctc
    10321 gtcctcgcag gacgtgctca accagattga agccatcacg aacccgaagg ccagaaagga
    10381 taagaagacg gtttcgtcga aacagcagca ggccaagagc gccatgcttg ccattctcga
    10441 cagggttcga gacgagtcga ctgaggaaaa gcccgtgcgc ctcgtcttcg aacccaagag
    10501 cagccgcatc gaccgcgagg agttcgtggc ggcgctcctt tcgcagacat cgatggagtc
    10561 caacgtctcc gtcaacctcg tcatgctcgg caatgacggc aagcccaagc agaagaacct
    10621 gctcgagatc atccgcgagt ggatcgcctt ccgcctgctg gccgttcgcc gccgcactca
    10681 ggcaaggctc gacaaggtca acgacaggat tcacgtgctt gagggtcgtg cgctcgtcct
    10741 tctcaacatc gaccgcgtca tcgagatcat ccgcaacagc gatgagccga aacccgctct
    10801 gattaaggag ttcggtctca ctgatcgtca ggcggacgac attcttgaaa tcaggctccg
    10861 ccagcttgct cgtctggccg ccattgcgat cgagaaggag ctcgccgaac tcaacaagga
    10921 aaagagttcg ctcgaacgca ttctcggcag tgacggcgtt ctgcgccgca cagtggccag
    10981 ggagatcgag caggcagcga agaccttcgg cgacgaccgc cgcaccctca tcgaggaggc
    11041 gagcgtcgtt caggccgaac agcacgtggt cgacgaaccc gttacggtcg tgctctcgca
    11101 gaagggcttc ctgagaaccc gtcagggcca cgggcacgac tgcacgctgc tcagctacaa
    11161 ggtgggcgac ggcttcgagt gtgcggtcga gtgtcgcacg gtcgacgaca tcagctttgt
    11221 caccgacacg ggccgtgtct acacctgcaa ggtctccgcc attcccggcg gtaggggcga
    11281 cggcttgccc gtcacgtcct tcttcgatct tgacaagggc atgaagcgat tcgtcggtgt
    11341 ctttgccggc cgtgagaagg aggatgtcgt gctcggcacg tcggacggcc tcgggctcaa
    11401 gtgcacgatc ggcgacctca aggcctcgca gcgcatcggc cgcaccttcg tgcgcatggc
    11461 cgacggcgtc acgctgatgc cgcccgccga gggctgcgcc gagatgccgc tgatcgcctg
    11521 tcttaccgag aagggccgtc tcgtgatctt cgcacacgac gagatccgca gactctccaa
    11581 cggcggcatg ggcgtttcgc tcgtgaagct tgagtccggt gaaaggatga tcggcatgac
    11641 gctcgtcgat gagcgcggtc tcaccgcctc cggcacggga cgaggcggca agcccagaga
    11701 ggtgcagatc acccgcaagt cctttgagga gttccgcatg cagcgcgccc gcaagggccg
    11761 ccagatcggc gtcacctgga cggtcgagtc gatcgcccct atcacgcccg tcaagcctga
    11821 aaacgaaggc gaaggctcgt cgggtgccgg ctccgcggga ggcctcacga tccagcttga
    11881 ggacgaaaat ccgcgcctca tctgacgcgc aaacggaaca caggaaggat ccacgtcgtg
    11941 aaactcaatc cgctccgctt tttcaagagc ctctcggcga ggcttctgct tctcacgctc
    12001 atctgggtga gcttcatcgt gaccacgatc gggtatacga tgatgctcaa ctggaagctt
    12061 gagagttcgt cggctgcgac gaatatcatc ggggacatca ggttccatgt cttccgcacc
    12121 gcgctttatg tgcttccgca gtatgacaac agggacttcg acaacgaagt ccgtacggtc
    12181 aacgcagggc ttgatcttct ccagaagggt gatcagtggc gtccgcttct cgtgcctgag
    12241 acgcaggcca tccgttcgtc gcttcagagc atcgactctg agtggaaaga gtcggtgctt
    12301 ccgcacctta cggccgcccg cggcggcgcc cgcgaaccga tgatgggcga cgtcaacctt
    12361 tatgtcgaga agctcgccgc gctgacgaac gacatcgacg aatatcgcgc gcacttcctg
    12421 tggcagctcc gctatctgca gggtctcctc atcgtgcttg cgatcggcag tctcttcgcg
    12481 atcatggcgc ttctgctccg ctgggtgatc cgtcccctcg agcagctcgg cggcgccatc
    12541 ggacggcttt cctcgggcga cctgacggcc cgcaccgaag tgaggagtga ggacgaaatc
    12601 ggcgagatcg ccgcaggctt caacgagatg gccgccagac tcaaggattc tcacgacaat
    12661 ctcgagcaga aggtagccga gaagacggcc agcgtcgaag aaaagaacag ccatctcgcg
    12721 cagctctacg agatgacctc ctatttcacg cagcgtcgca gccttgacga ccttgccgac
    12781 ggtttcgtct cgcgaatcat gcgccagacc gaggccgatg cgtgtacgct ccagctgctc
    12841 tcgggccgtg acgactcgat gcagctcctc acggccgagg gactttccgt cgacctcgtc
    12901 aaggcggtca acgatctgcc tggcgacgcg ggcatcgtgc cctcggtgct ctcgaagacc
    12961 tatcccgtct gcttccagct caccgagctt gaggacgagt tttccagacg ctttgccgac
    13021 gcgggtttca agacggccta cagcttccag atccgttcga cgccgggcga cctcggcatc
    13081 ttcacgctct tcttcaagga aagcccgcag ctcacgacgc aggtcatccg cctgctcgag
    13141 aacttcgtga cccacttcgg cgtcgccgtg gcgagcaacc gcctcatcga gcgcgaccgc
    13201 cagttcgccg tggtgcagga acgtcagctc cttgcacagg gcctgcacga ctcgatcgct
    13261 caggcgctct ccttcctcaa cctgcaggtg cagttcctgt cggacgccat ccgtactaac
    13321 gacgttgcgc tgcgtgacga atccctttcc gccatttgca ctggcgttca ggagtgctac
    13381 gaggacgtgc gcgaactcct tctcaacttc cgcgagcgtt tgcacaagga aggcttcatc
    13441 gagggcgtac ggaccgtgat tga
//
LOCUS       DBMDTI010000033        12720 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1520_length_12720_cov_3.344084, whole genome shotgun sequence.
ACCESSION   DBMDTI010000033 DBMDTI010000000
VERSION     DBMDTI010000033.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 12720)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12720)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12720
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1520_length_12720_cov_3.344084"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(173..1975)
                     /locus_tag="ACZPB6_03065"
     CDS             complement(173..1975)
                     /locus_tag="ACZPB6_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HIR1183488.1"
                     /translation="MRTAFKLLPVVAGIFAAAFAAPSHAVGGPSGLKVGYAVQGTLGE
                     TIVNPYGLAPLTAVIRNGGYVLKHATVRIVPKEGGQEIKYDVGPQTLRTHGGIPVFGL
                     YAGWRNTVEVTYTRVFQGEEKTVTESYVINTQPAWLETTGNPAIAQNFMTAKVTTPAP
                     KEFSDRLYFINNLGAADVRASRAVWNNPVGGALQWNNPPRNAILDTKGEIRWYMKADR
                     IYDPESLYDAGIMMGFHQNADGALSWGYGQHYAKYDLLGRKVFNRRLPANYADFSHAM
                     MPAENGGYFLRVASPDLRRADDTRVRTVRDVIIEVDASGEVVDEWRLFEILDPNRNIV
                     LKSIDQGAVCLNIDTSQSGKTLSAEQLAKLEAQHAFGDIAGTGAGRNCAHVNSVDHDP
                     VDDSIILSSRHQSAVVKIGRDKQVKWILAAPNGWRDGLKDKVLTPVGKDGRPLSCRIG
                     KCEGGFDWTWTQHTAWKIDELSKGDIVYVSVFDNGDGRAFVQPEDQNEKYSRAVVYKI
                     DQKAMTVEQVWEYGKERGHELYSPITSSVEYQADKNSVVVYWASIGTGSKTGPAPVLT
                     EFRWGETKPAFEMHLKGGMGYRALPIDAQKAFVR"
     gene            complement(2168..2713)
                     /locus_tag="ACZPB6_03070"
     CDS             complement(2168..2713)
                     /locus_tag="ACZPB6_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183489.1"
                     /translation="MTPQEAVSAFFNTMRSETDQAGLMYSQFAVVQALDYVIDRWEDL
                     NPLARDKAGNRTREWLEHLETVFGGMSTEELCAHKFDFSAELRDIADRGFTEDRVIPD
                     YRGDNFQRVAQARAILADGIYGSVDGIVAHIRDQGCPYALHATSLEIVRRLARIDVER
                     FGEDNCPGQLKMLETIESLRG"
     gene            complement(2897..2973)
                     /locus_tag="ACZPB6_03075"
     tRNA            complement(2897..2973)
                     /locus_tag="ACZPB6_03075"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2937..2939),aa:Pro,seq:ggg)
     gene            complement(3069..3551)
                     /locus_tag="ACZPB6_03080"
     CDS             complement(3069..3551)
                     /locus_tag="ACZPB6_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012442.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HIR1183490.1"
                     /translation="MKSFTESGVLFPDDWENEPEEPAVPPPAPEPKPERKVRDTAARP
                     APKLASEDEDLDYGTPCFKRADLVDLLCEEHGFDVAESRAFVAEFFDMIFDHLVQGHM
                     VKLSGLGRFSVRYKAPRPARNPWTGETVMLEERTVVCYQSSDTLNTRVTAKLREKKKG
                     "
     gene            complement(3611..6028)
                     /gene="pheT"
                     /locus_tag="ACZPB6_03085"
     CDS             complement(3611..6028)
                     /gene="pheT"
                     /locus_tag="ACZPB6_03085"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR1183491.1"
                     /translation="MLFTEEWLRQYCNPQLSTEELAETLTMAGLEVEEVTSVAPAFTG
                     VVVAEVLTCRDHENSDHLHVCEVNAGTGETLQIVCGAPNVRAGIKVACATIGAVLPGD
                     FKIKKSKLRGVVSMGMLCSARELGISEEHNGIWILPEDAPIGTDIREYARLDDSKIEI
                     KLTPNRGDALSVTGVARDLHAVTGAPLVMPAMDPVPATCECVKPVVIEAPDLCGRFTG
                     RVIRNLNPAAETPQWMRDRLERAGMRHISALVDISNYVMLELGRPTHFFDLAKLEGDR
                     ITVRWAREGESLKLLNEQTVELTPYYGVVCDGDTPSCIAGIMGGEKSSVSETTTDLFI
                     EAAFWQQLAIQGRCRKLNFSTDAAYRFERGVDFGTNAQHMEYVTRLVLEICGTSETRV
                     GPVDDQIVNLPERKPVTMRVARCLKVVGMDIPVEAMHNTFTRLGFEYTFDGEVFTVQA
                     PSHRFDIEIEEDLIEEVARLYGYEKLPDRPPLARIAMKSPKEASRTGHALRREMAVLG
                     YQELINFSFVPEAWEKDFAGNDAPIRLLNPIASQLSVMRTQLVGGLVDILKYNLNRKA
                     ERVRVFELGRVFFPDPAVLDGPWSVKGVRQPSHIAGLAYGTASDVQWGVEARRVDFYD
                     VKGDVERLVAPLKARFVKEVFPALHPGRSAAIYVGEKRVGFIGELHPKLVHEYGLPNA
                     PVVFELEVEPLLTLELPRYTPVSKFQPMRRDVAVVVPADFEVQVLMDAVNDARVSDAR
                     LGALVSFGLFDLYRPKTDEGDAEKSLAFAVELNSTGEEALTDAEADATMQALVEVLER
                     AGARLRA"
     gene            complement(6059..7075)
                     /gene="pheS"
                     /locus_tag="ACZPB6_03090"
     CDS             complement(6059..7075)
                     /gene="pheS"
                     /locus_tag="ACZPB6_03090"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR1183492.1"
                     /translation="MQDLSELIESARQDFAQAEQPAALEDAKAKYIGKSGAVTALMKS
                     LGQLAPEERRARGQEINKAKAAIEAALNARREQLAQAAMQAKLASEAIDVTLPGRVRT
                     EGGIHPVMRTWMRIEEIFRSIGFDVADGPEIESDWFSFTALNNPPNHPARSMQDTFYV
                     DRCDEEGRQLPLRPHTSPMQVRYARTHEAPIKIIAPGRTYRVDSDATHSPMFHQVEGL
                     WIGDDVSFTDLKGVYSDFLRRFFETDDLVVRFRPSYFPFTEPSVEVDMMFTSGPRKGK
                     WLEISGAGEVHPNVVRNFGLDPEKHIGFAFGSGLERLTMLRYGIDDLRLFFEGDLRFL
                     RQFN"
     gene            complement(7385..7852)
                     /locus_tag="ACZPB6_03095"
     CDS             complement(7385..7852)
                     /locus_tag="ACZPB6_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HIR1183493.1"
                     /translation="MTNHSTTSKNPFDIFIVGLRKGLDIGLYSLLPNVLMAFVLTYML
                     NLFGVMKFLGDTCGGVMGAFDLPGEAITVLLATWLSCGAGVGVAASLAAAGQLNAHDV
                     TILAPAFILMASQIQYMGRLLGVANCPKKYWPLLMFNSVFMACLGMIVMRFVA"
     gene            complement(7845..8516)
                     /locus_tag="ACZPB6_03100"
     CDS             complement(7845..8516)
                     /locus_tag="ACZPB6_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183494.1"
                     /translation="MADPKTQTACEENPKVGFGAYIALALAIIIFSGVFYKMPDAYKW
                     LGAFDYTTLIGKYGTIAGTASNFQGSGGFSARAGFLFSLNLFPGVMLAMGLIEVLSFY
                     GALRAAQVILTPLLKPILGVPGYTGLALITDLQSTDGGAAITRALHDEGRITTKNLVT
                     ICAWQYTGAGCVSNYYSTVSGLFTLFTVKVWIPMALILVLKFVGGALIRTILSTVYRK
                     DFEND"
     gene            complement(9216..9566)
                     /gene="rplT"
                     /locus_tag="ACZPB6_03105"
     CDS             complement(9216..9566)
                     /gene="rplT"
                     /locus_tag="ACZPB6_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR1183495.1"
                     /translation="MPRVKRGVTARARHKKVFALSKGFSLRRNNVYRVAKQAVMRAGQ
                     YAYRDRRDKKGTFRRLWIARINAGARANGMTYSVFMNGLKKAGIGLDRKVLSDMAIFD
                     KEGFASLVNQVKGA"
     gene            complement(9585..9782)
                     /gene="rpmI"
                     /locus_tag="ACZPB6_03110"
     CDS             complement(9585..9782)
                     /gene="rpmI"
                     /locus_tag="ACZPB6_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR1183496.1"
                     /translation="MPKMKTKRAAAKRFKPRASGSIKRAHATKRHILTKRTTKNKRQL
                     RGMVDIHESDIASVRRMLPYA"
     gene            complement(9947..10468)
                     /gene="infC"
                     /locus_tag="ACZPB6_03115"
     CDS             complement(9947..10468)
                     /gene="infC"
                     /locus_tag="ACZPB6_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR1183497.1"
                     /translation="MAQDRKHRINGEIRVPEVRLTGVDGAQIGIVRTAEALRMAEDAD
                     VDLVEIAPNAQPPVCRLMDYGKFRYQEQKKAQEAKAKQKVIQVKEVKFRPVTDENDFQ
                     TKLRSLVRFLNDGDKAKVTLRYRGREMAHQQFGMELMDRIRTELAEVAQVEHQPKLEG
                     RQMIMVLAPKKKK"
     gene            complement(10498..12417)
                     /gene="thrS"
                     /locus_tag="ACZPB6_03120"
     CDS             complement(10498..12417)
                     /gene="thrS"
                     /locus_tag="ACZPB6_03120"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR1183498.1"
                     /translation="MVAITLPDGSKREFAAAVTVADVAADIGAGLAKAALAGRVDGKL
                     VDLSHVIDKDAQVAIITGRDAEGVEIIRHSTAHLMAQAVKELYPEAQVTIGPAIENGF
                     YYDFSYSRPFTPEDLKAIEERMDEIVKRNIKIERIEMSRDEAVKFFTDLGEKYKAELI
                     EAIPQGETISLYRQGDFTDLCRGPHVPSTGALKVHRLMKVAGAYWRGDSKNEMLQRIY
                     GTAWATKDDLKAYLTMLEEAEKRDHRRLGRELDLFHLQDEAPGMIFWHPKGWALWQVI
                     EQYMRRIYQDNGYGEVKAPQLLDRSLWERSGHWAKYRDNMFTTESENRYYALKPMNCP
                     GHIQIFNCALRSYRDLPFRLGEFGQCHRNEPSGSLHGMMRVRGFTQDDGHIFCTEDQI
                     LAECEAFTKLVKDVYQDFGFTEIAYKVATRPEMRIGEDAVWDKAEHALMQSLDALGIK
                     YEVLEGEGAFYGPKIEYHLKDCLGRSWQCGTIQVDFQMPGRLGAEYVAEDNSRKVPVM
                     LHRAILGSLERWIGMLIEEYAGAFPTWLAPVQCAVACITDAQKDYADEVAAKLHAARI
                     RVQSDLRSEKINYKIRELSLQKIPYILVVGEKEKQAGCVAVRARGGKDLGSIKLDDFI
                     AMIKQEDEARSPFAH"
     gene            complement(12595..12671)
                     /locus_tag="ACZPB6_03125"
     tRNA            complement(12595..12671)
                     /locus_tag="ACZPB6_03125"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12635..12637),aa:Val,seq:gac)
BASE COUNT         2377 a         3637 c         4179 g         2527 t
ORIGIN      
        1 cctgaaattt ggagtgagcg ctactttttt ttcttggagg gagccgacca gactttttct
       61 taaaagtcaa ggccgcgttc cacggacttc cgagatgaac cgataaaaaa gggaggactc
      121 cctgcggaat cctccccttg tcatcctgtc ggcccgtcag gctgcaggag cgtcagcgga
      181 cgaaggcctt ctgcgcatcg atcggcagtg cgcgatagcc cataccgccc ttgaggtgca
      241 tctcgaaagc aggcttcgtt tcgccccagc ggaattccgt caggacgggc gcggggccgg
      301 tcttgctgcc cgtgccgatg ctcgcccagt agacgacgac cgaattcttg tcggcctgat
      361 actcgacgga ggacgtgatc ggcgagtaga gctcatggcc gcgttccttg ccgtactccc
      421 agacctgctc gacggtcata gccttctggt cgatcttgta gacgacggca cggctgtact
      481 tctcgttctg gtcctcgggc tgcacgaagg cgcggccgtc gccgttgtca aagacggaga
      541 cgtagacaat gtcgcccttg ctcagctcat cgatcttcca ggcagtgtgc tgcgtccagg
      601 tccagtcgaa gccgccctcg cacttgccga tgcggcacga gaggggcctg ccgtccttgc
      661 cgacgggcgt gaggaccttg tccttcagac cgtcgcgcca gccgttcggg gcggcgagga
      721 tccacttcac ctgcttgtca cggccgatct tgacgacggc ggactggtgg cgggaggaga
      781 ggatgatcga gtcgtcgacg gggtcgtggt cgactgagtt gacgtgggca cagttgcggc
      841 ccgcacccgt gcccgcgatg tcaccgaagg cgtgctgggc ctcgagcttg gcgagctgct
      901 cggcggaaag cgtcttgccc gactgcgacg tgtcgatgtt gaggcagacg gcgccctggt
      961 caatcgactt gaggacgatg ttgcggttcg gatcaagaat ctcgaagagg cgccattcgt
     1021 cgacgacctc gcccgaggcg tcgacctcga tgatcacgtc gcggaccgtg cgcacgcgcg
     1081 tgtcgtcggc gcgcctgagg tcgggcgaag cgacgcgcag gaagtagccg ccgttttcag
     1141 cgggcatcat cgcgtggctg aagtcggcgt agttggcggg caggcggcgg ttgaagacct
     1201 tgcggccgag tagatcgtac ttcgcatagt gctggccgta gccccaggag agcgcgccgt
     1261 cggcgttctg atggaagccc atcatgatgc ccgcgtcgta gagcgactcg ggatcataga
     1321 tgcggtcggc cttcatgtac cagcggatct cgcccttcgt gtcgagaatc gcgtttcgcg
     1381 gcggattgtt ccactgcagg gcgccgccca cgggattgtt ccagaccgcg cgactcgcgc
     1441 gcacatcggc tgcgccgaga ttgttgatga agtagaggcg atctgagaat tccttgggcg
     1501 cgggcgtcgt caccttggcc gtcatgaagt tctgggcaat cgcgggattt cccgtcgttt
     1561 caagccaggc aggctgcgtg ttgatcacgt agctttccgt cacggtcttc tcctcgccct
     1621 gaaagacgcg cgtataggtc acctcgaccg tattgcgcca gcccgcgtag aggccgaaga
     1681 cgggaatgcc gccgtgcgtg cgcagggtct gcgggccaac gtcgtacttg atctcctggc
     1741 caccctcctt cgggacgatg cgcacggtcg catgcttgag gacgtagccg ccgttcctga
     1801 tgacggcagt gaggggcgca agcccgtagg gattcacgat cgtctcgccg agcgtgccct
     1861 gcacggcata gcccaccttg aggccgctcg ggccgccgac ggcgtgggac ggagcggcaa
     1921 aggccgcggc gaagatgccg gcgacgaccg gcagaagttt gaatgcagta cgcactatcc
     1981 tctcctcttt ttctgagcgc cggctgcggc gtgcgcaggc ggcatccttc gatcttagca
     2041 aagcctgccc gatgaaagcg caagaagggg catgaaggct caaacgcaaa agtcccgcag
     2101 gcggacgccg gacgggactt cgggtacgcg tccgaatttc ggactcgcat atgtcgactt
     2161 atgcaaatca gccgcgcagc gactcgatcg tctcgagcat cttcagttgg ccggggcagt
     2221 tgtcctcacc gaagcgctcg acgtcgatgc gagcgaggcg acggacgatt tcaaggctcg
     2281 tggcgtggag tgcgtagggg cagccctggt ccctgatgtg cgcgacgatg ccgtcgacgg
     2341 agccgtagat gccgtcggcg agaatggcgc gggcctgtgc gacgcgctgg aagttgtcgc
     2401 ccctgtagtc ggggatcacg cggtcctcgg tgaagccgcg gtcggcgatg tcgcgaagct
     2461 cggcggagaa gtcgaacttg tgcgcgcaga gctcctcggt gctcatgccg ccgaagaccg
     2521 tctcaaggtg ctcgagccat tctcgagtgc ggttccccgc cttgtcgcgg gcgagcgggt
     2581 tgagatcctc ccagcggtcg atgacgtaat cgagcgcctg aacgacggcg aactgcgagt
     2641 acatgagacc cgcctgatcg gtctcgctcc tcatggtgtt gaaaaatgca ctgacggctt
     2701 cctgcggcgt catgacgtct ctccttgggt cgtggaatcg aaaggcgccc gccgcgtgcg
     2761 gacgcctgca tcatgaatgt tagaccgcga acctcaaggg aaccttacgg ctcgaaacga
     2821 ccggaggccc ccgggcgcta ggtacggatg aaaaaaaaga ggctgttcat ttctgaacaa
     2881 cctcttttcc tggatttggt cggggcggcg ggattcgaac tcgcgacccc ttgcacccca
     2941 tgcaagtgcg ctaccaggct gcgctacgcc ccgaaggatt tgaagtttat cctagcttgc
     3001 cgaaaaaagc aagagcattt cttcaaatac catatttacg tgatagccct gcctttttgc
     3061 aggcgttttc agcccttctt cttctcacga agcttcgcag tgacgcgggt gttgagagtg
     3121 tcggaggact ggtagcagac caccgtacgc tcctcgagca tgaccgtttc gcccgtccag
     3181 gggttccggg cgggtctcgg cgccttgtag cgcacggaga acctgcccag tcccgagagc
     3241 ttgaccatat gtccctgcac gagatggtcg aagatcatgt cgaagaactc cgccacgaag
     3301 gcgcggctct cggcgacatc gaacccgtgc tcctcgcaga gcagatcgac caggtccgcc
     3361 ctcttgaagc agggcgtgcc gtagtccagg tcttcgtcct cggacgcgag cttcggggcg
     3421 ggccttgcgg ccgtgtcacg caccttgcgc tcgggcttgg gctcgggggc gggcggcgga
     3481 accgcgggct cttcgggctc gttctcccag tcgtcgggga agaggacacc agattcagtg
     3541 aaggatttca taaggattct cgacctgcgc cctcacggag agggcgcaga tcgcaggatg
     3601 aagacgttga tcaagcgcga aggcgtgcac cggcacgttc gagcacctcg acgagggcct
     3661 gcatcgttgc atcagcctct gcgtccgtaa gggcctcctc gcccgtcgag ttgagctcga
     3721 cggcaaaggc aagcgacttc tcggcatcgc cctcgtcggt cttcggacga tagaggtcga
     3781 agagaccgaa agagacgagc gcgccgagac gggcgtcgga gacgcgggcg tcgttgacgg
     3841 cgtccatgag aacctgcact tcaaagtcgg caggtaccac gacggcgacg tcgcggcgca
     3901 tcggctggaa cttgctcacg ggcgtgtagc gcggcagctc gagcgtgaga agcggctcga
     3961 cctcaagctc gaagacgacc ggcgcattgg gcaggccgta ttcgtgcacg agcttcggat
     4021 gaagttcgcc gatgaagccg acacgctttt cgccgacgta gatggcggcg ctgcgacccg
     4081 gatgaagggc ggggaagacc tccttcacga agcgggcctt gagcggagcg acgaggcgct
     4141 cgacgtcgcc cttgacgtcg tagaagtcga cgcggcgggc ttcgacgccc cactgaacgt
     4201 cgctggccgt gccgtaggcg aggcctgcga tgtgggacgg ttggcgcacg cccttgacgg
     4261 accacggacc gtcaagcacg gcgggatccg ggaagaacac gcggccgagc tcaaagacgc
     4321 ggactcgttc ggccttgcgg ttgaggttgt acttcaggat gtcgacgaga ccgcccacga
     4381 gctgcgtgcg catgacggaa agctggctcg cgatcgggtt gagcaggcgg atcggcgcgt
     4441 cgttgccggc gaagtccttt tcccaggctt cgggcacgaa cgagaagttg atgagctcct
     4501 gatagccgag gacggccatt tcacggcgaa gcgcgtgacc cgtgcgggag gcctccttcg
     4561 gagacttcat cgcaatgcgg gcgagcggcg gacggtcggg aagcttttcg tagccgtaga
     4621 gacgggcaac ctcctcgatg aggtcctcct cgatctcgat gtcgaagcgg tgcgacggag
     4681 cctgaaccgt gaagacctcg ccgtcgaacg tgtattcgaa gccgaggcgt gtgaacgtgt
     4741 tgtgcatggc ctcgacgggg atgtccatgc cgaccacctt caggcagcgg gcgacacgca
     4801 tcgtgacggg cttcctttcg gggaggttga cgatctggtc gtcgaccggg cccacgcggg
     4861 tttcggacgt gccacagatc tcgaggacga ggcgcgtgac gtattccatg tgctgggcgt
     4921 tcgtgccgaa gtcgacgccg cgctcgaagc ggtaggcggc gtccgtcgag aagttgagct
     4981 tgcggcagcg gccctggatg gcgagctgct gccagaaggc ggcctcgatg aagaggtcgg
     5041 tcgtcgtttc ggagacggag ctcttttcgc cgcccatgat gccggcgatg cacgacggcg
     5101 tgtcgccgtc gcagaccacg ccgtagtagg gcgtgagttc gaccgtctgc tcgttgagca
     5161 gcttcaggct ttcgccttcg cgggcccagc gcacggtgat gcggtcgcct tcaagcttgg
     5221 cgaggtcgaa gaagtgcgtc ggacggccga gctcgagcat gacgtagttc gagatgtcga
     5281 cgagcgcgga gatgtggcgc atgccggcgc gctcgaggcg gtcgcgcatc cactgcggcg
     5341 tctcggcggc gggattgagg ttgcggatca cgcggcccgt gaagcggccg cagaggtcgg
     5401 gggcttcgat cacgacgggc ttgacgcatt cgcacgtggc gggcaccgga tccatggcgg
     5461 gcatgacgag cggagcgccg gtcacggcat ggagatcgcg ggcgactccc gtaacggaga
     5521 gcgcgtcgcc gcggttcggc gtgagcttga tctcgatctt ggagtcgtcg agcctggcgt
     5581 actcgcggat gtccgtgccg atcggcgcgt cttccggaag aatccagatg ccgttgtgct
     5641 cttcgctgat gccgagttcg cgggcggagc agagcatgcc catggacacg acgccgcgca
     5701 acttggactt cttgatcttg aagtcgcccg gcaggacggc gccgatcgtg gcgcaggcga
     5761 ccttgatgcc ggcgcgcacg ttcggggcgc cgcagacgat ctggagcgtt tcgcccgtgc
     5821 cggcattcac ttcgcagacg tgcagatggt cggagttctc atgatcgcgg caggtgagga
     5881 cctcggcgac gactacgccc gtgaaggcgg gagccacgct cgtgacctcc tcgacttcaa
     5941 ggccggccat cgtgagcgtt tcggcaagct cttcggtgct gagctgcgga ttgcaatatt
     6001 gacgcagcca ctcttctgta aacagcattt cagtaattcc tttcgatggt tggcccggtt
     6061 agttgaactg acgcaggaag cgcaggtcgc cttcgaagaa gaggcgcagg tcgtcgatgc
     6121 cgtagcggag catcgtgagg cgctcgaggc cggagccgaa cgcgaagccg atgtgctttt
     6181 caggatcgag gccgaagttg cgcacgacgt tcgggtgcac ttcgccggcg cccgagatct
     6241 cgagccactt gcccttgcgc gggccgctcg tgaacatcat gtcaacttca acggacggct
     6301 cggtgaacgg gaagtaggac ggacggaagc gaacgacgag gtcatccgtc tcgaagaagc
     6361 ggcgcaggaa gtcgctgtag acgcccttga ggtcggtgaa gctcacgtcg tcaccgatcc
     6421 acaagccttc gacctgatgg aacatcgggg agtgcgtagc gtcggagtcg acgcggtagg
     6481 tgcggcccgg ggcgatgatc ttgatcggag cctcatgcgt gcgggcgtag cgaacctgca
     6541 tcggagaggt gtgcggacgc agcggcagct ggcggccttc ctcgtcgcag cggtccacgt
     6601 agaacgtgtc ctgcatggag cgggcgggat ggttgggcgg gttgttgagg gccgtgaagc
     6661 tgaaccagtc gctttcgatt tcgggaccgt cggcaacgtc gaagccgatg gagcggaaga
     6721 tctcctcgat gcgcatccat gtgcgcatga cggggtggat gccgccttcg gtgcggacgc
     6781 ggcccggaag ggtcacgtcg atcgcttcgc tggcgagctt ggcctgcatg gcggcctgag
     6841 cgagctgttc gcggcgggcg ttgagcgcgg cttcgatagc ggccttggcc ttgttgattt
     6901 cctgaccgcg ggcgcggcgc tcttcgggcg cgagctggcc gaggctcttc atgagagccg
     6961 tcacggcgcc gcttttgccg atgtacttgg ccttggcgtc ttccagggca gcgggctgct
     7021 ctgcctgagc gaagtcctga cgcgcggact cgatgagttc ggaaagatcc tgcatgttca
     7081 ctgatctcga ctttgatgtt gtaaaaccgg gcgggatcac ccgtccggtc catggcggcc
     7141 gcgcatcctc atccgagggt aacgcctgac gcggaggatg cctgctgcga cagcctgttg
     7201 cgaaaaaagg cccgaatggt ctccggcttc ttttcgcaag aggctcccat cgcaagagag
     7261 gaccgacgaa aaatcttatt ttacaacgac ttgtctgcat tcccaaaatt ttgaagaacc
     7321 ggaaagcgcg aaagccggcg gctaaagccg tcggctttcg ggttgtgatt gctcagggac
     7381 gcggtcaggc gacgaagcgc atcacgatca tgccaaggca ggccatgaag acgctgttga
     7441 acatgagcag cggccagtac ttcttcgggc agttggccac gccgagaaga cggcccatgt
     7501 actggatctg ggaggccatc agaatgaagg ccggcgcgag aatcgtcacg tcatgggcgt
     7561 tgagctggcc ggcagcggcc aggctggcgg caacgccgac accggcgccg caggagagcc
     7621 acgtggcgag aagcaccgtg atggcctcgc ccggaaggtc gaaggcgccc atcacgccgc
     7681 cgcaggtgtc gccgaggaac ttcatgacgc cgaagagatt cagcatgtac gtgagcacga
     7741 aggccatcag cacgttcggc agcaggctgt agagaccgat gtcgagaccc ttgcgcaggc
     7801 cgacgatgaa gatgtcgaag ggattcttcg aggtggtgga gtgattagtc attttcgaag
     7861 tccttgcgat aaacggtgct gaggatggtg cggatcagag cgccgcccac gaacttgagg
     7921 accagaatga gggccatcgg gatccagacc ttcacggtga agagcgtgaa gagaccggag
     7981 acggtggagt agtagttgga cacgcagccc gcgcccgtat actgccaggc gcagatcgtc
     8041 acgaggttct tcgtcgtgat gcggccttcg tcgtgcaggg cgcgcgtgat ggcggcgccg
     8101 ccgtcggtgg actgaaggtc ggtgatgagg gcaaggcccg tgtagcccgg cacgccgagg
     8161 atcggcttga ggagcggcgt caggatgacc tgagcggcac gcagagcgcc gtagaaggag
     8221 agcacctcaa tgaggcccat ggcgagcatg acaccgggga acagattgag ggagaagagg
     8281 aagcccgcgc gggcgctgaa gccgccggag ccctgaaagt tgctggccgt gccggcgatg
     8341 gtgccgtact tgccgatgag ggtcgtgtag tcgaaggcgc cgagccactt gtaggcgtcg
     8401 ggcatcttgt agaagacgcc ggagaagatg atgatggcaa gcgcgagcgc gatgtacgcg
     8461 ccgaagccca ccttcggatt ctcctcgcag gcagtctgag tcttgggatc cgccatgagt
     8521 cttgtccttt ttcggaggga gggttgaagt gaagacgatt gaatcgttct tctgcgaaag
     8581 gcgccccagc gacggcggtg atccgttgtc ggggtttacc acgcatcgca gtatacctgc
     8641 gccgactttc ggactgcctc gggcgtttgt actagacgcc ccgacgcccg gacaagactt
     8701 cggcaggagc ttcgacgaac cccaccgaag gcgcagtcgt tttcgtcact ttgacgagaa
     8761 cagacacccc tacgtctagg cacaacgccc tagggaaaat tctgatgcct ttcggcctct
     8821 ttttttcatg aaaaagatga gagcaaagca cgcccccctg cggcccgaag tcgcatcatg
     8881 aaagattcgc aggaaccaag ccctcatcac cacaccgaga tctgcgtcgc acccgtatct
     8941 cttcatgagc tgatctgaat ggttggcgat gcacctgctc cttatttttc atcatagatg
     9001 ggtgatgaac ggtttgtcat catgtccatt ttttttgctc aaaagcgaca gaccgcaggc
     9061 tttcacggga aagcgctgcg gcctcggagt cctcgaagga cggaaaaaag ggcgaaaaaa
     9121 aagagaccca gcgaataccg agtctcttct tctcaaagct tccggatgaa agcaagacga
     9181 ttcagtgcaa accgtccgtc cggacatgct tcgaattagg cacccttgac ctggttgacg
     9241 agggaggcga agccttcctt gtcgaagatg gccatgtcgg aaaggacctt gcggtcgaga
     9301 ccgatgccgg ccttcttgag gccgttcatg aacacggagt aggtcatgcc gttggcgcga
     9361 gcgccggcat tgatacgggc gatccacaga cggcggaacg tacccttctt gtcacggcgg
     9421 tcacgatagg catactgacc ggcgcgcatg acagcctgct tggcaacacg gtagacgttg
     9481 ttgcggcgga ggctgaagcc cttggaaagg gcgaaaactt tcttatgacg ggcacgagcc
     9541 gtcacaccac gcttaactcg gggcatcttc tatatctcct ttaattaggc gtaaggcagc
     9601 atgcgacgca cggaagcgat atcggattca tggatatcga ccatgccgcg gagctggcgc
     9661 ttgttcttcg tcgtacgctt cgtaaggata tgacgcttcg tagcgtgggc acgcttgatc
     9721 gagccactgg cgcgcggctt gaaacgcttg gcggcagcgc gcttagtctt catcttcggc
     9781 atttgccgtt acctctttat ttgatgaacc acaggcgccc catccagacg acgggaactt
     9841 tctcaagccc gaggctcact tgttatccaa aaaacggacc gacttgcggt ccgttttttg
     9901 ggaaagacag aattctacct caattttgag gcagtgtgtg aaaaaatcac tttttctttt
     9961 taggagccag caccatgatc atctggcggc cttcgagctt gggctgatgt tcaacctgag
    10021 ccacttcggc gagctccgtg cggatgcggt ccatcagctc catgccgaac tgctggtgag
    10081 ccatttcacg gccgcggtag cgcagcgtga ccttggcctt gtcgccgtca ttgaggaatc
    10141 gcaccaggga gcggagcttc gtttggaagt cgttttcgtc cgtcaccggg cggaacttca
    10201 cttccttgac ctgaatcacc ttctgcttgg ccttggcttc ctgagccttc ttctgttcct
    10261 gataacggaa cttgccgtag tccatcaggc ggcagaccgg cggctgggcg ttcggcgcga
    10321 tctcaacgag gtcaacgtcg gcgtcctcag ccatgcgcag ggcttccgca gtgcggacaa
    10381 tgccgatctg ggcgccatcg acgccggtca gtcggacctc aggaactcgg atctcaccgt
    10441 tgatccgatg cttgcgatct tgtgctatga caatctccta aatcgttagt tttgcggtta
    10501 gtgcgcaaag ggcgagcggg cttcgtcctc ctgcttgatc atcgcgatga agtcatcgag
    10561 cttgatcgag ccgaggtcct tgccaccgcg ggcgcgaacc gccacgcaac cggcctgctt
    10621 ttccttctcg cccacgacga gaatgtacgg aatcttctgc aggctcaatt cgcggatttt
    10681 atagttaatc ttttcgctgc gcaaatcaga ctgcacgcgg atgcgggcag cgtgaagctt
    10741 ggcggcgact tcgtcggcgt agtccttctg ggcgtccgtg atgcaggcga ccgcgcactg
    10801 gaccggggcg agccaggtcg ggaaggcgcc ggcgtattct tcgatcagca tgccgatcca
    10861 gcgttcgaga gaaccgagga tggcgcggtg aagcatgacg ggaaccttgc gcgagttgtc
    10921 ttcggcgacg tactcggcgc cgaggcggcc cggcatctgg aagtccacct ggatggtgcc
    10981 gcactgccaa gagcgaccga ggcagtcctt aagatgatat tcgatcttgg gaccgtagaa
    11041 ggcgccttca ccctcaagaa cttcatactt gatgccgaga gcgtcaagac tctgcatcag
    11101 ggcatgctcg gccttgtccc agacggcgtc ctcaccgatg cgcatttcgg ggcgcgtggc
    11161 gaccttgtag gcgatctcgg tgaagccgaa gtcctggtag acgtccttga cgagcttcgt
    11221 gaaggcttcg cattcggcga gaatctggtc ttccgtgcag aagatgtggc cgtcgtcctg
    11281 ggtgaagccg cgaacacgca tcatgccatg gagcgagccg gagggttcgt tgcggtggca
    11341 ctggccgaat tcgccgaggc ggaacggcag atcacggtag ctacgcagcg cgcagttgaa
    11401 gatctggatg tggcccgggc agttcatcgg cttgagggcg tagtagcggt tctcggactc
    11461 ggtcgtgaac atgttgtcac gatacttggc ccagtgaccg gaacgttccc agagcgagcg
    11521 gtcgagaagc tgcggagcct tgacttcgcc gtagccgttg tcctggtaga tgcggcgcat
    11581 gtactgctcg atcacctgcc agagggccca tcccttcgga tgccagaaga tcatgcccgg
    11641 ggcctcgtcc tggaggtgga agaggtcgag ctcgcggccg aggcgacggt ggtcgcgctt
    11701 ttcggcttcc tcaagcatcg tcagataggc cttgaggtcg tccttcgtgg cccaggcggt
    11761 gccgtaaatg cgctggagca tctcgttctt ggagtcgccg cgccagtagg cgccggcaac
    11821 cttcatcaga cggtgcacct tgagcgcgcc cgtggacggc acatgggggc cgcggcagag
    11881 gtcggtgaag tcgccctgac ggtagagcga gatcgtttca ccctgcggga tggcttcaat
    11941 gagttcggcc ttgtactttt cgccgagatc cgtgaagaac ttcacggctt cgtcgcggga
    12001 catctcgatg cgttcgatct tgatgttgcg cttgacgatc tcgtccatgc gctcctcgat
    12061 ggccttgagg tcctcggggg tgaacggacg gctgtaggag aagtcgtagt agaagccgtt
    12121 ttcaatggcc ggcccgatcg tgacctgggc ttccgggtaa agctccttca cggcctgcgc
    12181 catcagatgg gcggtcgagt gacggatgat ttcaacacct tcggcgtcgc ggcccgtgat
    12241 gatggcgacc tgagcgtcct tgtcgatcac atgggagagg tcgacgagct tgccgtcgac
    12301 gcggccggcg agagcggcct tggccagacc ggcaccgatg tcggcggcga catcggcaac
    12361 ggtgacggcg gcggcgaatt cgcgcttgga gccgtccgga agggtaattg caaccatttt
    12421 taacctcgtt ttctacaggc gaaaaaaaag tgcggacatg agccgcactt ctttgccttg
    12481 gacattttgt tggacaacgt ttcgctcacg ccctcaattt tttcaaagag agggtgttgt
    12541 tgtgcgaaaa gtctgcgaag aacacatcgt tcaacaaaat cccaaataaa attctggtag
    12601 gcacgattgg attcgaacca acgaccccca ccatgtcaag gtgatgctct aaccaactga
    12661 gctacgtgcc tgtcaaggtt tttattatgc ctgagatttt gcgcatttgc aaactcttgc
//
LOCUS       DBMDTI010000034        12718 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1522_length_12718_cov_2.991202, whole genome shotgun sequence.
ACCESSION   DBMDTI010000034 DBMDTI010000000
VERSION     DBMDTI010000034.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 12718)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12718)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12718
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1522_length_12718_cov_2.991202"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="ACZPB6_03130"
     CDS             <1..650
                     /locus_tag="ACZPB6_03130"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HIR1183499.1"
                     /translation="KQFLFEGAQGSMLDIDHGTYPYVTSSNTVAGSACAGAGVGPHML
                     NYVLGITKAYCTRVGEGPFPTELHDEVGQMLREKGCEFGAVTGRPRRCGWFDGAALRR
                     AVQINGLTGLAVMKLDVLDGMETVKLGVGYKYRGEEIAIMPAGADAVAECEPIYEEFP
                     GWKESTFGVTKWDELPETARQYLTRLSEVAGAPIALVSTGPDREQTILHVDPFEA"
     gene            845..1372
                     /locus_tag="ACZPB6_03135"
     CDS             845..1372
                     /locus_tag="ACZPB6_03135"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106130 - purine phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR1183500.1"
                     /translation="MSDISYTWEEYIDLNEKLVAMVAKSGWQFDSILCLSRGGMRPGD
                     FFSRVYDMPLAVLATSSYREAKGTVQSDLDISDCITGVSELKGRVLLVDDMVDSGKTI
                     REVIEHLKKRYPGITEIRVAVLWYKAHSVLVPDWHVEYLEDNPWIHQPFECYDALGPA
                     KLIELVEAKEAAKKA"
     gene            1757..2101
                     /locus_tag="ACZPB6_03140"
     CDS             1757..2101
                     /locus_tag="ACZPB6_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183501.1"
                     /translation="MKIASAYSVHLRNFCRVFDDTVEVYRKAVDFFIEIMLRHRDPKF
                     TELKHDNDCIRLAEGLNVVNKDIAVKTANFIVKLANEFKVDVIVMEHRDLTGRKRGTK
                     RQRLHHWRAKYV"
     gene            2567..4003
                     /locus_tag="ACZPB6_03145"
     CDS             2567..4003
                     /locus_tag="ACZPB6_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR1183502.1"
                     /translation="MTQPVTPDLTNAGMDVTELAVGAKDVIASTAERFTSVIEGLLQH
                     LSLENVLMQIAAVFVSMAIGYVISRRINTLVDRVLPGEDVRGLGAYARRALVAFTHNV
                     SFSFISGSLLALIVYCIVKIFEFAPQSMVIARVCYNILYAFALLSLLMAFLQGGIGLR
                     MITPGVRRIVTIIFWVMAVLQFFGILTDIIQYLDTTVIPIGGGSMTVWKLLVAIVSVL
                     LTLGVANWLASLVNQMVEGLENMSENIKVVLKRVVTVIFMVLAVIIALGTVGIDLTIL
                     SVFGGAVGVGLGFGLQKIASNYISGFIILLDKSIKIGDLVTVGGFKGRVTEINTRFTV
                     VRNNDGVENIVPNESFVTSAVLNHSYTESTSVQYIDISVAYDADVERALAIMLEEGMR
                     ARPRIDTGRRGWAYLDTFGDSGINLRLGFWVKDPVNGVAGLKTAISLAILKRFAEEGI
                     EVPYNQLEVNLRKVDVGEIPVRMKVQSS"
     gene            complement(4125..4559)
                     /locus_tag="ACZPB6_03150"
     CDS             complement(4125..4559)
                     /locus_tag="ACZPB6_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR1183503.1"
                     /translation="MITTTRFPYWIERTADGSYRARFPSIPEILPTAPSIAKLVEGLQ
                     DEVLPALEARLKEDRLPEPREPRAGEEVFSLSPNFASKLHLIVALKKEKLSGSELARR
                     LGCLPQETHRLLKLSHPTKIDTISAALSALGGELTCSVTFRH"
     gene            complement(4653..4739)
                     /locus_tag="ACZPB6_03155"
     tRNA            complement(4653..4739)
                     /locus_tag="ACZPB6_03155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4703..4705),aa:Leu,seq:cag)
     gene            4851..7262
                     /locus_tag="ACZPB6_03160"
     CDS             4851..7262
                     /locus_tag="ACZPB6_03160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R family protein"
                     /protein_id="HIR1183504.1"
                     /translation="MAKKKKTPVAQIEPISLPDEDLAKARAWLEGLNADIAYSQAKRQ
                     LAEACGWERSKSNAVIVALHEEGFMAGEKNYFCNPNAPAEPGVVRGAREVSNFTIMLQ
                     SDPEVSVPLPYAIHCLPGDVFMLRKTVTGNWRVSNFVARHQTRWVCKLRGRIRRGRRS
                     GIAQVVPINGFAPVEMQMDLADVPAEVDLEKAAFEVEFLPESMKPEPYVEIFVRFVKE
                     IGNRFDPLGEIAIASAEYDLPVEFSAAALDEAQALPDEVDPKNMGRRVDLRDIPFVTI
                     DGEDARDFDDAVYCARVEDGRTRLLVAIADVSHYVKPGAPLDVDAQQRATSVYFPASV
                     VPMLPEKLSNGLCSLNPGVDRLTMVCDAVIDPEGRTEAYQFYPAVIHSHARLTYTQVW
                     GALQGEEGGLAAVGDRLDDIRALYDLFKTLRKARDARHTLDLETKETMAVFDDKGVIS
                     EFKVREHNDAHRLIEECMLVANVCAADFVIQKKRGALFRVHDAPSQERLETLRTVLKS
                     FNEKLESPTPEGFAELISRTKENEFLQTAILRSMSRACYSPDNVGHYGLQYEAYAHFT
                     SPIRRYPDLLLHRAIKGILSRRIYVPQVVFDDSSLMVSRQARGLGSRPEAGDGDKPAT
                     QAEKRHSVWERLGILCSAAERRADDATRDVMNYLKCDYMLRHGKGRHEAVVTGMIPAG
                     VFVALKDIAVDGFIHISNLGWGYYEFDEKNLTMTSREEMTQVRVGDRVIVRLEEVDLE
                     NRRMSFVLESNLERRLIKGGKGGSRRSSRRGARLYGRKFDPFDIDDDDFDELFGQEGD
                     DDWDD"
     gene            7376..8122
                     /gene="rlmB"
                     /locus_tag="ACZPB6_03165"
     CDS             7376..8122
                     /gene="rlmB"
                     /locus_tag="ACZPB6_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR1183505.1"
                     /translation="MKNIVLAGFHAVGARLRMDPKSISAVYLDPERRDKRMREMREKL
                     IAAGVRVMTADNRRLDGMAPDVPHQGIVAMAEEKQAQLEFDELLDLITDDTLILILDG
                     VTDPRNFGACLRVADAAGVQCVLVPRDRSAHMSPAALKAAAGAAETVPVVTVTNLAAS
                     IVELRDAGVTVVGTAGEATKSIYEFDQKKAFAWVLGAEGDGLRQLTRRRCDDLAAIPM
                     AGAVESLNVSVASGICLYESVRQRTGGKLK"
     gene            8261..8644
                     /gene="rpsF"
                     /locus_tag="ACZPB6_03170"
     CDS             8261..8644
                     /gene="rpsF"
                     /locus_tag="ACZPB6_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR1183506.1"
                     /translation="MRHYELCILVHPDQSEQVPAMIERLKTLVAEQNGKIERVEDWGR
                     RQLAYPIEKLVKAHYVLMNIECGHETIEEIENGFRFNDAILRHLTVKTKHAETAPSVM
                     MKAVEKEEAKKVAAAEAAAAEASAE"
     gene            8690..8974
                     /gene="priB"
                     /locus_tag="ACZPB6_03175"
     CDS             8690..8974
                     /gene="priB"
                     /locus_tag="ACZPB6_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HIR1183507.1"
                     /translation="MNLIEISGTLTSKEEVRYTPAGIPVFEGVFHHRSELTEANRIRK
                     LEYDFPAIAFGALAESLNKAQMGQDVNIRGFLAPRSMKSSKLIVHITELN"
     gene            8986..9252
                     /gene="rpsR"
                     /locus_tag="ACZPB6_03180"
     CDS             8986..9252
                     /gene="rpsR"
                     /locus_tag="ACZPB6_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR1183508.1"
                     /translation="MAFKGKGKPRRANQQNSLFKRKKFCRFTAEGVETIDYKDIDTLK
                     DFIQENGKIMPARLTGTKAHYQRQLDTAIKRARFLALLAYTDNQ"
     gene            9274..9726
                     /gene="rplI"
                     /locus_tag="ACZPB6_03185"
     CDS             9274..9726
                     /gene="rplI"
                     /locus_tag="ACZPB6_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR1183509.1"
                     /translation="MQVILLEKIAHVGDLGDVVKVKDGYGRNFLIPTGRAKRATKAAI
                     AEFEARRAELEAAQAERIAAAQKIADTINEGEIEISSKCGVDGRLFGSVTNADIAEAI
                     DAKYGLQIKKSQVRTPLGAIKAVGDYVITIGLLTDITADVTVHVVGEA"
     gene            complement(10421..10933)
                     /locus_tag="ACZPB6_03190"
     CDS             complement(10421..10933)
                     /locus_tag="ACZPB6_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HIR1183510.1"
                     /translation="MLDWFAQIDWFAVICWLSAVSLIIAGLAGTVIPAIPGLPLIAAG
                     GALIGWAGGFELIGWGTIATLAVLAIIGVIVDTVAQTAGAQKAGASSYGIWGSVIGTF
                     VGVFMGGLVGIFIMPLVGAFIGEFWAKRDYLHAGRVGVATWIGIVVGTAVKIALAFTM
                     LGLMIVMYFV"
     gene            complement(10952..12343)
                     /gene="dnaB"
                     /locus_tag="ACZPB6_03195"
     CDS             complement(10952..12343)
                     /gene="dnaB"
                     /locus_tag="ACZPB6_03195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR1183511.1"
                     /translation="MTDLIDDPAAQAVRRPPQSINSEQSILGGLMIDNNALDSIVDII
                     QPDDFYRRDHKIIYEHIAAMIQRGRPADSLTVAESLRDAGLDTDTGGFAYLAELVNNT
                     PSAANIRRYAEIVHDKSVLRQLISAGDHMVTCALQPEGRETAQILDEAERIVLAINER
                     NSRGQRGFQPMATLVREVSEKIIDLYQNQQGSEVTGVPTGYPNLDRELAGLQRGDLII
                     IAGRPSMGKTSFAINIAENVGVRQELPVAVFSLEMGGDQLAQRLISSVANIDAQKLRK
                     AQLEDEEWNAFSKAVHRLENKPIYIDDTPALMISELSSRARRLVNQTGPLGLIVVDYI
                     QLMTGRAGVDNRSTELSEISRGLKALAKELHCPVIVLSQLNRSLEQRADKRPIMSDLR
                     ESGAIEQDADVIMFIYREVVYNKDTPDKNLAEIIIAKQRNGPIGTLRMVFRGGNTRFE
                     PWAGDVGYWEGAQ"
     gene            complement(12424..>12718)
                     /locus_tag="ACZPB6_03200"
     CDS             complement(12424..>12718)
                     /locus_tag="ACZPB6_03200"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: protein motif:HMM:NF014040.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HIR1183512.1"
                     /translation="EIKSGNASPHTGEIIQLCAYAILLEQLHGGRIDIGFILYETKSK
                     HKEILFTDDVRRKLDFCVEAIQRDAAKALVPQTSASDAQCTQCEFMNFCADRL"
BASE COUNT         2609 a         3809 c         3690 g         2610 t
ORIGIN      
        1 gcaagcagtt cctcttcgaa ggcgcccaag gctcgatgct cgacatcgac cacggcacgt
       61 acccgtacgt cacgagctcc aacaccgtcg ccggctctgc ctgcgccggc gccggcgtcg
      121 gtccgcacat gctcaactac gtcctcggca tcaccaaggc ctactgcacc cgcgtcggcg
      181 aaggtccgtt cccgaccgaa cttcacgacg aagtcggcca gatgctccgt gaaaagggct
      241 gcgagttcgg cgccgtcacg ggccgtcccc gccgctgcgg ctggttcgac ggtgccgccc
      301 tgcgccgcgc cgttcagatc aacggcctca ccggcctcgc cgtcatgaag ctcgacgtcc
      361 tcgatggcat ggaaaccgtc aagctcggcg tgggctacaa gtaccgcggc gaagagatcg
      421 cgatcatgcc cgccggcgcc gatgccgtcg ccgaatgcga accgatctac gaagaattcc
      481 cgggctggaa ggagagcacc ttcggcgtca cgaagtggga tgaactcccc gagaccgccc
      541 gtcagtacct gacgcgcctc tccgaagtcg ccggcgcccc gatcgctctc gtgtcgaccg
      601 gtcctgaccg cgaacagacg attcttcacg tcgacccgtt cgaagcctga actccgttag
      661 actgaaacca tgaaaaaaac ggcgggaagc tcatgcttcc cgccgttttt ttcatctatt
      721 tcgccccttc ttgaccgaaa cctctcaaca aggcgaactt tttggtctaa aatcccgtct
      781 ttcatttctc ggatcccgcc catcttcgcg ggtccgctca cttggcacgt tcagagagac
      841 acacatgtcg gacatttctt acacctggga agagtacatc gaccttaacg aaaagcttgt
      901 tgccatggtc gccaagagcg gctggcagtt tgacagcatc ctttgcctgt ctcgcggcgg
      961 catgcgcccg ggcgacttct tcagccgcgt ctacgacatg cctcttgccg ttctcgccac
     1021 gagctcctac cgcgaagcca agggcacggt ccagtccgac ctcgacatct ccgactgcat
     1081 caccggcgtc tccgaactca agggccgcgt ccttctcgtc gacgacatgg tcgactccgg
     1141 caagacgatc cgcgaagtga tcgagcacct caagaagcgc taccccggca tcaccgagat
     1201 ccgcgtcgcc gtcctctggt acaaggccca ctccgtcctc gtccccgact ggcacgtcga
     1261 atacctcgag gacaatccct ggatccacca gccgttcgaa tgctacgacg ccctcggccc
     1321 ggccaagctc atcgagctcg tcgaagcaaa ggaagccgcc aagaaggcct gaggaccggt
     1381 cggggcgtca catttcgaga gcttttcggc tcttgaacac gcctcgcgct gagttatact
     1441 gaccagagcg caaagccgtt ttcggaactt tccggaagcg gcttttgaac tgatttccca
     1501 tctatgcgaa gcatgggggc aagcggggct gatgaagtct gtatgggtgt cgattgatcg
     1561 ttgatccact taagttgcgc cgaaacgcaa tgagggtgat tttgatttgg tatatgatct
     1621 gtgcggggtg atgggtaatc aggcttaaaa agcatactaa tgttcgattt taatgtagtg
     1681 ctttttaagc ctgtccaaaa aaatcaaaac ttggtagcat ggatccatat tccagttaac
     1741 cagactgcag atttcgatga agattgcgtc cgcctattcc gtgcacctgc gcaacttctg
     1801 ccgcgtcttc gatgatacgg tcgaggtgta tcgcaaggca gtggactttt tcatcgagat
     1861 catgctgagg caccgggatc cgaaattcac ggaactcaag catgacaacg actgcattcg
     1921 acttgcagag gggttgaacg ttgtcaataa ggacattgcc gtcaagacgg caaacttcat
     1981 cgtgaagctt gcgaatgaat tcaaggttga cgtcatcgtc atggagcacc gcgatcttac
     2041 gggtcgaaaa cggggcacca agcggcaacg gctgcatcac tggcgggcca agtatgtcta
     2101 gaagatggtg gagcacaagg cgcatcggtg cggcattcgc attcgtcgcg tctgcgcctg
     2161 gaacacgtcg aagcttgcct acggcggctc gggtcctgtc gcaagagaca cggagaacta
     2221 cagcatgtgt acgttcaaga cagggaagcg ccatgagtgt gatctgtcgg cttcgtacaa
     2281 tatcggatct cgctacttcc ttcgggagta cgagaagtcc attccggcaa cggaatggcg
     2341 acgcatttcg gcaaaagttc cggagtgcgc ccataggatc cctcgcaccc tgaacactct
     2401 aattagggta tgtgcggagc tgcactccaa gtgagcgcag agttctgagc attcccgcta
     2461 tctgtggtta gggagccctc tggtaagtcg gagggaaggc acgtccatgc gaccgaaagg
     2521 gaggatgggc gtggaagatt ctcaatactc atactgagca acaacaatga ctcaacctgt
     2581 tacgcccgac ctgaccaacg ccggcatgga cgtcacggaa ctcgccgttg gtgcgaagga
     2641 cgtcatcgcc tcgacggccg agcgcttcac gagcgtgatc gaggggctgc tccagcacct
     2701 ctcgcttgaa aacgtgctca tgcagattgc ggccgtcttc gtcagcatgg cgatcggcta
     2761 cgtgatctcc cgccgcatca acacgctcgt cgaccgcgtg ctccccggcg aggacgtgcg
     2821 cggtctcggc gcatacgccc gccgcgcgct cgtcgccttc acgcacaacg tttccttcag
     2881 cttcatctcg ggctctcttc tcgccctcat cgtctactgc atcgtcaaga tcttcgaatt
     2941 tgcgccgcag tcgatggtca tcgcccgagt ctgctacaac atcctctacg cctttgcgct
     3001 tctctcgctc ctcatggcct tccttcaggg aggcatcggg ctgcgcatga tcacgccggg
     3061 cgttcgccgc atcgtcacga tcatcttctg ggtgatggcc gtgctgcagt tcttcggcat
     3121 cctcaccgac atcatccagt acctcgatac gacggtcatc ccgatcggcg ggggcagcat
     3181 gaccgtctgg aagcttctcg tcgcgatcgt ctccgtcctc ctcacgctcg gcgtcgcgaa
     3241 ctggctcgca tccctcgtca accagatggt cgagggcctc gagaacatgt ccgagaacat
     3301 caaggtcgtc ctcaagcgtg tagtcaccgt aatcttcatg gtcctcgccg tgatcatcgc
     3361 gctcggcacc gtcggcatcg acctcacgat cctctcggtc ttcggtggtg cggtcggtgt
     3421 cggtctcggc ttcggtctgc agaagatcgc ctcgaactac atctcgggct tcatcatcct
     3481 gctcgacaag tcgatcaaga tcggcgacct ggtcacggtc ggcggcttca aggggcgcgt
     3541 gacggaaatc aacacccgct tcaccgttgt gcgcaacaac gacggcgtcg agaacatcgt
     3601 gccgaacgag tccttcgtga cgagcgcggt gctcaaccac tcctacaccg agtccacgtc
     3661 cgtccagtac atcgacatct cggtcgccta cgacgccgac gtcgagcgcg ccctcgcgat
     3721 catgctcgag gaaggcatgc gcgcgcgtcc gcgcatcgat acgggccgcc gcggctgggc
     3781 ctatctcgac accttcggcg actcgggcat caacctgagg ctcggcttct gggtgaagga
     3841 tcccgtcaac ggcgtcgccg gtctcaagac cgccatctcg ctcgccattc tcaagcgctt
     3901 tgccgaagag ggcatcgagg tgccgtacaa ccagctcgag gtcaacctgc gcaaggtcga
     3961 cgtcggtgaa attccggtcc gcatgaaggt ccagtcctcc tgacgatcgt caccgacaac
     4021 tcggaaatgt tcaacgagaa agggcgcgcc ttccgcgagg cgcgcccttg ttatttcgat
     4081 tgctgcggtc ggacttgagc gcggacgcgg ttgcttttga ccagtcaatg tctgaaggtg
     4141 accgaacagg tgagttcgcc accgagcgct gagagcgccg cggagatcgt gtcgatcttc
     4201 gtcggatggc tgagtttcag aagacggtgg gtttcctgcg gcaggcagcc cagacgcctg
     4261 gcaagctcgg agccgctcag cttctccttc ttcagcgcga cgatgaggtg aagcttgctc
     4321 gcgaagttgg gggagagcga gaagacttcc tcgcctgcgc ggggctcgcg cggctcgggc
     4381 agcctgtcct ccttgagcct tgcctcaagt gcgggcagca cctcgtcctg cagcccctcg
     4441 acgagctttg cgatgctcgg cgccgtaggc agaatttccg gaatgctcgg aaagcgcgcg
     4501 cggtagctgc cgtcggctgt gcgttcaatc cagtagggga agcgtgttgt tgtgatcatg
     4561 gacgataatg gtgttctgct gtcgacagtc aggatagcac gggctgaaat gaaaaaaggc
     4621 ctgtactttc gtacagacct tttagaattt ggtggtgccc ggaagaggac tcgaacctcc
     4681 acggccgtga agccgccagc acctgaagct ggtgcgtcta ccaatttcgc catccgggca
     4741 accttgagga tttgaattat gcacttgctt ttcgagtttg tcaagaattt ctaaaaatat
     4801 ttttttgagt tggctgtaga ctgtcgcaat gcatgaacaa cagaggtgtt atggctaaaa
     4861 agaagaagac gccagtggcg cagatcgaac cgatttccct tcctgacgag gatctcgcca
     4921 aggcgcgtgc ctggcttgag gggctcaatg ccgacattgc ctactcgcag gcaaagcgcc
     4981 agcttgcaga agcctgtggc tgggaacgct cgaagtccaa cgccgtcatc gtggccctcc
     5041 acgaggaggg cttcatggcg ggcgaaaaga actatttctg caatccgaac gcgcctgccg
     5101 aacccggcgt cgtgcgcggc gcccgcgagg tctcgaactt cacgatcatg ctccagagcg
     5161 atcctgaggt ttcggttccc ttgccctacg caatccattg cctgcccggt gacgtcttca
     5221 tgcttcgcaa gacggtcacg ggcaactggc gcgtttcgaa cttcgtcgca cgtcatcaga
     5281 cgcgctgggt ctgcaagctt cgcggccgca tccgccgcgg ccgtcgttcg ggtatcgcac
     5341 aggtcgttcc gatcaacggc ttcgccccgg tcgagatgca gatggacctt gcagatgttc
     5401 ccgccgaggt cgatctcgag aaggcggcct tcgaggtcga gttcctgccc gaatcgatga
     5461 agcccgagcc ctacgtcgag atcttcgtgc gcttcgtcaa ggagatcggc aaccgcttcg
     5521 atccgctcgg cgaaatcgcc atcgcctcgg ccgaatacga cctgcccgtt gaattcagcg
     5581 ctgcggcgct tgacgaggct caggccctgc ctgacgaagt cgatcccaaa aacatgggtc
     5641 gccgcgtcga cctgcgcgac atcccgttcg tgacgatcga cggcgaggat gcccgtgact
     5701 tcgacgacgc cgtctactgc gcgcgcgtcg aagacggcag aacgcgcctt ctcgtcgcca
     5761 tcgccgacgt gagccactac gtcaagcccg gtgcgccgct cgacgttgac gcgcagcagc
     5821 gcgccacgag cgtctacttc cccgcgagcg tcgtgccgat gctgcctgag aagctctcga
     5881 acggcctctg ttcgctcaat cccggcgtcg atcgcctgac gatggtctgc gatgcggtca
     5941 tcgatcctga gggcaggacc gaggcctatc agttctatcc cgccgtcatc cactctcacg
     6001 cacgcctcac ctacactcag gtctggggcg cgctgcaggg cgaggaaggg ggactcgccg
     6061 ccgtcggcga tcgccttgac gacatccgtg cgctttacga tctcttcaag acgctgcgca
     6121 aggcccgcga tgcacgacat acgctcgacc tggagacgaa ggagacgatg gccgtctttg
     6181 acgacaaggg cgtcatcagc gaattcaagg tgcgcgaaca caatgacgcg caccgtctca
     6241 tcgaggagtg catgctcgtc gcgaacgtct gcgccgcaga cttcgtgatc cagaaaaagc
     6301 gcggggcgct cttccgtgtt catgacgcgc cgagtcagga gcgtctcgag acgctgcgca
     6361 ccgttctcaa gagcttcaac gagaagctcg agtcgccgac gcccgagggc ttcgcggagc
     6421 tcatctcgcg cacgaaggaa aatgaattcc tgcagaccgc catcctgcgc tcgatgtccc
     6481 gtgcctgcta ctcgcccgac aacgtcggtc actacgggct gcagtacgag gcctacgccc
     6541 acttcacgtc tccgattcgt cgctatcccg acctgctgct tcatcgtgcg atcaagggaa
     6601 ttctctcgcg tcgcatctat gtgccgcagg tggtcttcga cgactcgtcc ttgatggtct
     6661 cgcgtcaggc ccgaggcctc ggcagcaggc ctgaggcagg ggacggagac aagcccgcaa
     6721 cgcaggccga gaagcgccac agcgtctggg agcgcctcgg cattctctgc tccgcggcgg
     6781 agcgccgcgc cgacgacgcg acgcgcgacg tgatgaacta tctcaagtgc gactacatgc
     6841 ttcgccacgg caagggccgt cacgaagcgg tcgtcacggg catgattccc gcgggcgtct
     6901 tcgtcgccct gaaggacatc gccgttgacg gcttcatcca catttcgaac ctcggttggg
     6961 gctactacga attcgacgag aagaacctca cgatgacgag ccgcgaggag atgacgcagg
     7021 tgcgcgtcgg cgaccgcgtc atcgttaggc ttgaggaagt cgaccttgag aaccgccgca
     7081 tgtccttcgt gctcgagtcg aacctagagc gccgtctgat caagggcgga aagggcggct
     7141 cgcgccgttc ttcgagacgc ggtgcgaggc tctatgggcg caagttcgac ccgttcgaca
     7201 ttgatgacga tgacttcgac gagctgttcg gccaggaagg cgatgacgac tgggacgatt
     7261 gagcccgttt cgagctgacg ctcgaaacat cgtgcgctac aatgagcgca aatttcaaaa
     7321 cctctgcgcg ctgttggttt gctcgacagc gcgcttttaa tagtagttgc tgtaagtgaa
     7381 gaacattgtt ttggcaggct ttcatgccgt gggcgcccgt ctgcgcatgg acccgaagtc
     7441 catctccgcc gtctaccttg atcccgaacg ccgcgacaag cgcatgcgcg agatgcgcga
     7501 gaagctgatc gccgccggcg tgcgcgtgat gaccgccgac aaccgtcgcc ttgacggcat
     7561 ggctcccgac gttccgcacc agggcatcgt tgcgatggcc gaggaaaagc aggcccagct
     7621 cgagttcgat gaactgctcg atctgattac cgacgacacg ctcatcctga tccttgacgg
     7681 cgtgaccgac ccgcgcaact tcggcgcctg cctgcgcgtc gccgacgccg ccggcgtcca
     7741 gtgcgtactc gtgccgcgcg accgttccgc ccacatgtcg cccgccgccc tcaaggccgc
     7801 cgccggcgct gcggaaacgg tgcccgtcgt cacggtgacg aacctcgccg cctcgatcgt
     7861 tgaactgcgc gacgccggcg tgaccgtcgt gggtactgcc ggtgaagcca cgaagtcgat
     7921 ctacgaattc gaccagaaga aggcgttcgc ctgggtgctc ggcgccgagg gcgacggcct
     7981 gcgccagctg acccgccgcc gctgcgacga ccttgccgcc attccgatgg ccggcgccgt
     8041 cgagagcctg aacgtgtccg tcgcctcggg tatctgcctc tatgaatccg tgcgccagcg
     8101 cacgggcggc aagctcaagt aatctttttt ccgggcatgc gattttttcc gcttttcggg
     8161 tggcgaaagc ccgagaaagt gagtaaaatc gcatgctttc tgtccttgct gcaccggagt
     8221 agacgcccgg gcagctttca tccgcaagga gacaataaca atgcgtcact acgaactttg
     8281 cattctcgtg catcctgatc agagcgaaca ggttcccgcc atgatcgaac gcctcaagac
     8341 ccttgtcgcc gaacagaacg gcaagatcga acgcgtcgaa gactggggcc gtcgtcagct
     8401 cgcctacccg atcgaaaagc tcgtcaaggc tcactatgtt ctcatgaaca tcgaatgcgg
     8461 ccatgagacg attgaagaaa tcgaaaacgg cttccgtttc aacgacgcca tcctgcgtca
     8521 cctcaccgtc aagacgaagc atgccgaaac ggctccgtcc gtcatgatga aggctgttga
     8581 aaaggaagaa gccaagaagg tcgccgctgc tgaagccgct gccgccgaag cttccgccga
     8641 ataattcagt ctcgactgaa cagatttttc tgtgcgatct gagatctgag tgaacctcat
     8701 cgagatttcc ggcacgttga cttccaagga ggaagttcga tacacgccgg cgggcattcc
     8761 ggtttttgag ggtgtctttc accatcgatc cgaactgacc gaagcaaaca ggatcagaaa
     8821 actcgagtac gacttccccg ccatcgcctt cggcgctctg gccgagagcc tgaacaaggc
     8881 tcagatggga caggacgtga acatccgagg ctttctggcg ccgcgctcga tgaagagttc
     8941 caaactgatc gtacacatca ccgaattaaa ttaaggagct acaaaatggc tttcaagggt
     9001 aagggcaagc cccgtcgcgc taaccagcag aactctctct tcaagcgcaa gaagttctgc
     9061 cgctttacgg ccgaaggcgt tgaaacgatc gactacaagg acatcgacac gctcaaggac
     9121 ttcatccagg aaaacggcaa gatcatgccg gctcgtctga cgggcacgaa ggctcactat
     9181 cagcgtcagt tggacaccgc catcaagcgt gcccgcttcc tcgctctcct cgcctacacc
     9241 gacaaccagt aatcagattt aaggagatat caaatgcagg ttattcttct cgagaagatt
     9301 gctcacgttg gtgacctcgg cgacgtcgtc aaggtcaagg atggctacgg ccgcaacttc
     9361 ctgatcccga ccggccgtgc caagcgcgcc acgaaggctg ccatcgccga atttgaagcc
     9421 cgtcgtgccg agctcgaagc cgctcaggcc gaacgtatcg ctgccgccca gaagatcgcc
     9481 gacacgatca acgaaggcga aatcgaaatc tcttccaagt gcggcgttga cggccgtctc
     9541 ttcggttccg tcacgaacgc tgacatcgcc gaagccatcg atgccaagta cggtcttcag
     9601 atcaagaagt cccaggtccg cacgccgctt ggcgccatca aggctgtcgg tgactacgtc
     9661 atcacgatcg gtcttctcac cgacatcacg gctgacgtca cggtccacgt tgttggcgaa
     9721 gcctaattcg cttccgcttc aactgacttg aactcaaaaa aaaggcccgc agaactgcgg
     9781 gcctttttgt catgtgcgcc tagcagtgca cgtgtgtcta tgcgatgaaa gttcgctgtc
     9841 cgcccgaaca ggggaaggac tagcaactcg gcaagtcggg gaaggcaacg acttcgatgg
     9901 aggaagccga tagcaaagca ccagcctgac gaacaggaat cggatgtgag gcgtgtatct
     9961 cgggagggtc ggcatgcatc gattaacccg aaactgttac ggaagtttca gagcagatgc
    10021 tggcttgagt tattacgcgt ctctacaaat aggatgtctc caagaagaga gcccatagct
    10081 cttgctagag agattttgga aacctatttg ttgagattac taataagatg taacctccgt
    10141 cagcgcctgc ttgatctgag tgagagagtt tcagagcaga tgctggcttg agttaagatg
    10201 taacttaggc gtcgcctcat agcgcgagaa agcgcgaagt ttcagagcag atgctggctt
    10261 gagttaagat gtaacgtggg ctttgcctgc tctcatcaat ggggactaca gtttcagagc
    10321 agatgctggc ttgagttaag atgtaacgta tcaaggatca acttcgtgcc gctattctca
    10381 ggtttcagag cagatgctgg cttgagttaa gatgtaactt ttatacgaag tacatcacga
    10441 tcatcagccc gagcatggtg aatgccagag cgattttgac ggcagttccg acgacaatgc
    10501 cgatccaggt cgcaacacct acgcgccccg cgtgaagata atctcgtttg gcccagaact
    10561 cgccgatgaa tgcaccgacg agcggcatga tgaagatgcc tacaagaccg cccatgaaga
    10621 caccgacgaa ggtaccaatg accgaacccc agatgccgta ggagctggca ccggccttct
    10681 gtgccccggc ggtctgcgcc acagtgtcga cgatgacgcc gatgatggca agaacggcga
    10741 gcgtcgcgat cgtgccccag ccgatcagtt cgaaaccgcc tgcccatccg atcagggcgc
    10801 ctcctgcggc gatcagcgga agtccgggaa tggctgggat gacggtgccg gcgaggccgg
    10861 cgatgatcag cgaaacggca ctcagccagc agatgacggc aaaccagtct atttgtgcaa
    10921 accagtcgag cattcgaaat tcctctttag gttactgtgc gccttcccaa tagccgacat
    10981 cgcctgccca gggctcgaag cgggtgttgc cgccgcggaa gaccatgcgg agcgtaccga
    11041 ttggaccgtt acgctgcttg gcgatgatga tctcggcaag attcttgtca ggagtgtcct
    11101 tgttgtagac gacctcgcga taaatgaaca tgatgacgtc ggcgtcctgt tcgattgcgc
    11161 cggattcgcg aaggtcggac atgatcgggc gcttgtcggc tcgctgttca agagagcggt
    11221 tgagctgcga gagcacgatg accgggcagt gcagctcctt tgccagcgcc ttgaggccgc
    11281 gtgaaatttc agacagttcg gtcgagcggt tgtcgacgcc ggcacggccc gtcatcagct
    11341 gaatgtagtc gacgacgatg aggccgagcg ggcccgtctg gttgaccaga cgtcgagcac
    11401 ggctcgaaag ctctgagatc atcagggcgg gcgtgtcgtc gatgtagatg ggcttgtttt
    11461 ccagacgatg gacggccttc gagaaggcgt tccattcctc gtcctcgagc tgcgccttcc
    11521 tgagcttctg ggcgtcgatg ttggcgacgg agctgatcag acgctgggcg agctggtcgc
    11581 cacccatttc gagagagaag acggcgacgg gcagttcctg acgcacgccg acgttttctg
    11641 caatgttgat ggcgaacgag gtcttgccca tcgagggacg gcccgcgatg atgatcaggt
    11701 cgccgcgctg cagacccgca agctctctgt cgagattggg atagcccgtc ggcacgcctg
    11761 tgacttccga gccctgttgg ttctggtaaa gatcgatgat cttctcggaa acttctcgaa
    11821 cgagcgtggc catgggctgg aagccgcgct ggccgcgaga attgcgttcg ttgatcgcga
    11881 gcacgatgcg ctcggcctca tcgaggatct gcgccgtttc gcgtccttcg ggctgcagcg
    11941 cgcatgtgac catgtggtcg cccgcactga tcagctgtcg cagaacggac ttgtcgtgaa
    12001 cgatttcggc gtagcggcgg atgttggcag cggagggcgt gttgttgacg agttccgcaa
    12061 gatacgcaaa gccgcccgta tcggtgtcga ggcctgcatc gcgaagcgac tcggcgacgg
    12121 tcagtgagtc agcagggcgg ccgcgttgga tcatcgccgc gatgtgctca tagatgatct
    12181 tgtgatcgcg gcggtagaag tcatcgggct gaatgatgtc gacgatgctg tcaagagcat
    12241 tgttgtcgat catcaggccg cccagaatgg actgctcgct gttgatgctc tgcggaggcc
    12301 tgcgcacggc ctgcgcggcg ggatcgtcaa tcaagtccgt catgtgttcg cttcatcctc
    12361 atgtgtgagc ccactatctt agcgtcaaat tcctcaagag gggtgcttga ataggttcaa
    12421 aacttacagc ctgtccgcgc aaaaattcat gaattcacac tgcgtgcatt gagcgtccga
    12481 ggccgaagtc tgcggtacaa gtgctttggc tgcgtcacgc tggattgctt caacgcagaa
    12541 gtcgagtttt cgccgaacgt catcggtgaa gagaatttcc ttgtgcttgg atttggtctc
    12601 gtaaagaatg aagccgatat cgatcctgcc gccatgcagt tgttcaagca aaatggcgta
    12661 tgcacaaagc tgaatgatct cgcccgtgtg cggggaagcg tttccggact tgatctcc
//
LOCUS       DBMDTI010000035        12160 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1593_length_12160_cov_3.512729, whole genome shotgun sequence.
ACCESSION   DBMDTI010000035 DBMDTI010000000
VERSION     DBMDTI010000035.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 12160)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12160)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12160
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1593_length_12160_cov_3.512729"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="ACZPB6_03205"
     CDS             <1..1064
                     /locus_tag="ACZPB6_03205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR1183513.1"
                     /translation="TAMAVCLNSRLASRVLMKVAEASYWDTRDIYEMAADTPWEKWFG
                     PDLTIKVSANGQRCPLESIDFAVLRIKDGICDRFRDLAGRRPDVERYNPDVRIAAFLT
                     YDTCTFYLDLAGEALFKRGWRLTHGEAPLKENLAAGLLMLSGWDPSQTLIDPFCGSGT
                     IGIEAAQMAAGMAPGLNRRFAFEKLEGFDVDLWAEMKEDARAAVNRHVRIRIAGSDIS
                     SLVVEKAEENARRAGLGDMLDDGRLTFRQGDAREACPEEGMAPGLLIANPPYGEQSNP
                     KSASIQSMMKHVADNLKANFAGWTAWMLTSDRTLPQQMRLKESRKTVLFNGPLECRFF
                     RFDMVAGSNRRKPCEEADE"
     gene            complement(1192..1560)
                     /gene="rplS"
                     /locus_tag="ACZPB6_03210"
     CDS             complement(1192..1560)
                     /gene="rplS"
                     /locus_tag="ACZPB6_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR1183514.1"
                     /translation="MNLIQILEQEEMARVTAGKTIPSFRAGDTVVVNVNVVEGSRKRV
                     QAFEGVVIARRNRGLNSSFIVRKISSGEGVERTFQLYAPTIASIEVKRYGDVCRSKLY
                     YLRDRSGKAARIKELLVRKN"
     gene            complement(1647..2387)
                     /gene="trmD"
                     /locus_tag="ACZPB6_03215"
     CDS             complement(1647..2387)
                     /gene="trmD"
                     /locus_tag="ACZPB6_03215"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR1183515.1"
                     /translation="MRFDVVTLFPEIFDSITKCGITARAMKRGLWSCHTWNPRAMTED
                     VHRTVDDRPFGGGPGMVMMADPLAKTVDMIRASGNRGRVISFAPNGTKLTDERVRRMV
                     AEDESVVLVCGRYEGIDERFLSTYVDEVVSLGDFVLSGGEIPACALMDAVVRQLPGAI
                     KDLSTSDESFATGLLDAPHYTRPEVWRGMEVPSVLLSGHHANISRWTREESLRFTLQT
                     RPDLIKIADSSGKLSDADRKWLRANGWK"
     gene            complement(2399..2917)
                     /gene="rimM"
                     /locus_tag="ACZPB6_03220"
     CDS             complement(2399..2917)
                     /gene="rimM"
                     /locus_tag="ACZPB6_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435707.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR1183516.1"
                     /translation="MDDLVELGRTAGAYGFRGWVRIVPLESGEVLQKARTWVLTDLKG
                     VRTTLSIEAFRRHGDGFIAKWAGCETKEAADAARGRVSVSRADFPDAGKDAVWAVDLV
                     GCKVVNKEGTALGTVESIGSNGAQDLLVIAYDNADGKKAVFMLPLVKDVYLIAIDTDV
                     RTITVDWQPDWR"
     gene            complement(3133..3381)
                     /gene="rpsP"
                     /locus_tag="ACZPB6_03225"
     CDS             complement(3133..3381)
                     /gene="rpsP"
                     /locus_tag="ACZPB6_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR1183517.1"
                     /translation="MVIVRLARGGSKKRPFYSINVCDSRCRRDGRFIERIGYYNPMAA
                     GQAVRLNVNQERLAYWQSQGAQLSETVARLVKEAKAAA"
     gene            complement(3615..4550)
                     /locus_tag="ACZPB6_03230"
     CDS             complement(3615..4550)
                     /locus_tag="ACZPB6_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR1183518.1"
                     /translation="MTALLIAEHDNVTLKSATAQALTAALRMTDVVDVLVAGSNGQSV
                     AEQAAKLSGVRTVYYADHPQLAQESPEVLARLVLDEAEGYSHVLFVASAVGKSAMPRV
                     AAKLDVSPVSDVLDVKGPKTFVRGIYAGSLLATVEVTDPVVVATIRTTAFEAAAQEGS
                     AVVEEVIAPEGYDASRFVRFSEAKSDRPELVSARIVVGGGRGLLDEAGFKTLEAFADS
                     IGAAVGATRTAVDMGLCPNDWQVGQTGKMIAPELYIAVGISGAIQHTAGIKDAKTVVA
                     INKDAEAPIFEVADYGLVADGVEALEALRARLGTK"
     gene            complement(4572..5321)
                     /locus_tag="ACZPB6_03235"
     CDS             complement(4572..5321)
                     /locus_tag="ACZPB6_03235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR1183519.1"
                     /translation="MKILVAVKRVVDAKIKVRPLPDHSGVDVKLAKMAMNPFDEIAIE
                     KAVTLREAGVADEVVAVTVGPLKAVDTLRVAMAIGADRAVHVRTDESLEPLAVAKVLR
                     AVVEREKPDLVLLGKQAIDDDANQTGQMLSALCDMPLATFANEIVLEGGRWVVTRETD
                     GGTQTVSLATPAVVTADLRLAEPRYVTLPAMGKARKKPVEDIELAGLGIDTAPRIRIL
                     GVEEPPARKAGVMVSGVDELVDRLKNEAHVI"
     gene            complement(5490..6818)
                     /locus_tag="ACZPB6_03240"
     CDS             complement(5490..6818)
                     /locus_tag="ACZPB6_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR1183520.1"
                     /translation="MLTGLVLVYLIVLMWFGFCGERHSSSGRAFLTSGGRAGALLCAL
                     SLVSTIIGGSATLGMGSLAQKIGSGAFWWLGVGAVGLLLHGWIAPKIRALPAVTLPEV
                     VGVVAGRNAERWAGVIIAVSWIAVTAAQFVALHTLLLTLSSPLAAEVLYVMIAGVVIV
                     HTITGGQSAVIRTDAFQAVLLLGGFTAAAFWLLGDRPDAVMALDPVPLGDRFGPWDLG
                     RMMLLVGITYVVGPDMFSRTFTARDGQSARFAAWIASPCLVWFGVVVTGLALLNLQDP
                     QPVAGWLSSASEMPAWLKGALALGLISALCGSADTVLLSASGIVERSLLAGDRTNAVR
                     FFVGVFGFAAAAAVYVSKDIIWLLLTAYSFFVPGVALPLLIALIGRVRRLNAQLWTAG
                     AVFGGIGGLVGNVTGDEVWTFAGMGVSAAFAVASRFKAPAGGSDAFGSLR"
     gene            complement(6863..7303)
                     /locus_tag="ACZPB6_03245"
     CDS             complement(6863..7303)
                     /locus_tag="ACZPB6_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HIR1183521.1"
                     /translation="MQDFLFEWLSGAGAEAAVFASAFISSTLMPGGSEALLAGALSQT
                     PDDTPRLCRLVLLATAGNTLGAMTTWLIGRLIPEREKTDRALVWLRRWGAPTLLLSWL
                     PVVGDALPLAAGWLRMPAASCAVWVAVGKLLRYVVVAAGTLSLV"
     gene            complement(7406..8476)
                     /locus_tag="ACZPB6_03250"
     CDS             complement(7406..8476)
                     /locus_tag="ACZPB6_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HIR1183522.1"
                     /translation="MANIQFKNIRKSYGDVAVVHGISLDIRDGEFIVIVGPSGCGKST
                     LLRMVAGLEQITSGELIIGERVVNNLEPKDRDCAMVFQNYALYPHMTVFDNMAYGLKI
                     RGVPKDEIERRVNEAAKMLELGGLLARRPRQLSGGQRQRVAMGRAIVREPSVFLFDEP
                     LSNLDAKLRVQTRLQIQKLHRRLKTTSLYVTHDQVEAMTLAHRMIVMNAGRAEQVGTP
                     QEVYDDPETMFVANFIGSPPMNLVHGRVSADGSHFEHDSGVLSLPEPCTALAGRAVVL
                     GVRPEHVTLRPEGGLAAFEVETVEALGADHLIHGVLLGQNVVFRVDNHMYIPEVGIRA
                     GLEFSAEHVYWFDPETTRRIRL"
     gene            complement(8489..9337)
                     /gene="ugpE"
                     /locus_tag="ACZPB6_03255"
     CDS             complement(8489..9337)
                     /gene="ugpE"
                     /locus_tag="ACZPB6_03255"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HIR1183523.1"
                     /translation="MVENRPWLNFFTHLILILGVIVVVFPVYVAFVASTQTAVEVEQA
                     PMSLLPGAHFIENYMQILTEGGGTASRAPVGRMLFVSLMMAMGISIGKILISLVSAFA
                     IVYFRFPGRKLCFWLIFITLMLPVEVRISPTYEVMADLHLLDSWGGLILPVIASATAT
                     FLFRQFFLTVPDELTEAARMDGAGPMRFFFTILMPLSLTNIAAMFVIQFIYGWNQYLW
                     PLLVTTSEDMYPIVIGIKRMIGGGDSANEWNLIMATAMLAMIPPAIVVTVMQRWFVKG
                     LVDTEK"
     gene            complement(9351..10232)
                     /gene="ugpA"
                     /locus_tag="ACZPB6_03260"
     CDS             complement(9351..10232)
                     /gene="ugpA"
                     /locus_tag="ACZPB6_03260"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HIR1183524.1"
                     /translation="MEKRVVFQNRWLPYLLVAPQLAITLIFFFLPAGQAIYQSVLMQD
                     PFGLSTEFVGLQNFETLLSDPNYVSSFKITMVFSALVAFLGLAISLLLAVCADRVLRG
                     ASAYKTFLIVPYAVAPAVAGVLWMFIFNPTLGVLSYVMRMFGIDWNYLVDADQALTLL
                     VIAAVWKQVSYNFIFFLAGLQAIPKSLLEAAAMDGAGPFRRFWTVVFPLLSPTTFFLL
                     VMNIVYAFFDTFAIVDATTMGGPGQDTNILVYKVFVDGFRNMDFGGSAAQSVILMVMV
                     IILTVIQFRFIERRVNY"
     gene            complement(10390..11706)
                     /gene="ugpB"
                     /locus_tag="ACZPB6_03265"
     CDS             complement(10390..11706)
                     /gene="ugpB"
                     /locus_tag="ACZPB6_03265"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HIR1183525.1"
                     /translation="MSKKFVAAGVMAASLMAAGAASAAPTEITMWHAMANTLGDWVAD
                     VTKDFNAKTPQCHLTSTYKGTYDQTMTSGIAAHRAGRSPNILQVFEVGTATMMFSKGA
                     IVPVGELLDKAGYEFNPKDYIPAVYGYYSTADGRMLSYPFNSSTTVLYLNLTKFKNAG
                     LPTEPDKLPRTWPEIEKAAKALKAAGETCPMTTSWMGWTQLESFSTWHNVEYATLNNG
                     FGGTGARLMVNSPLHQRHLENLARMAKEGTFVYKGRGNLADATFYSGECAINMGSSGS
                     LGNIRRNAKFEYMTMPMPYYEGVPDAPQNTAIGGASLWAMAGKSDEQNRCVAEFFHYL
                     SDPQVQANNHMRTGYLPVTQKAFEIAKASGYYEKNPGADVAVKQMLKTTDKSRGIRLG
                     YLPNIRTIVDEEFEKIWSGKATSKEALDAVVRRGNEQLVRFERVTK"
     gene            complement(11991..>12160)
                     /locus_tag="ACZPB6_03270"
     CDS             complement(11991..>12160)
                     /locus_tag="ACZPB6_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR1183526.1"
                     /translation="MRALFERLRVKHPGMDTLSMGMSADMDEAVSEGATMVRVGRAVF
                     GPRDYSAKCAV"
BASE COUNT         2433 a         3725 c         3880 g         2122 t
ORIGIN      
        1 ccacggcgat ggccgtatgc ctcaactccc gcctcgccag ccgcgtgctc atgaaggtcg
       61 ccgaggcctc ctactgggac acccgcgaca tttatgaaat ggctgcagac accccttggg
      121 agaagtggtt cggtcccgac ctgacgatca aggtctcggc caacggccag cgctgcccgc
      181 tcgaatcgat cgacttcgcc gtgctccgca tcaaggacgg catctgcgac cgcttccgcg
      241 acctcgcggg acgtcgtccc gacgtcgagc gatacaaccc cgacgtgcgc atcgccgcct
      301 tcctcaccta cgacacctgc accttctatc tggatcttgc gggcgaagcg ctcttcaagc
      361 gcggctggcg tctcacccac ggcgaggctc ccctgaagga aaaccttgcg gcaggtcttc
      421 tgatgctctc gggctgggat ccctcccaaa cactcatcga tccgttctgc ggctcgggca
      481 cgatcggaat cgaagctgcg cagatggctg caggcatggc gcctggcctc aaccgccgct
      541 tcgccttcga aaagctcgag ggctttgacg tcgacctctg ggccgaaatg aaggaagatg
      601 cgcgcgccgc cgtcaatcgg cacgtcagga tccgcattgc aggcagcgac atctcatccc
      661 tcgtcgtcga gaaggccgag gaaaacgccc gccgcgcggg cctcggcgac atgctcgacg
      721 acggacgcct taccttccgt cagggcgacg cccgcgaagc ctgtcccgaa gaaggcatgg
      781 cgcccggact tctcatcgca aacccgccct acggtgaaca gtccaacccg aagtccgcct
      841 ccatccagtc gatgatgaag cacgtcgccg acaatctgaa ggccaacttc gcaggctgga
      901 ccgcctggat gctcacctcc gaccgcacgc tcccgcagca gatgcgcctc aaggagtcgc
      961 gcaagacagt tctcttcaac ggaccgcttg aatgccgctt cttccgcttt gacatggttg
     1021 cgggcagcaa ccgcagaaag ccgtgcgaag aggcggacga ataagtccct ccgttctcct
     1081 cgcaacagag cccccgtgca cagcatgggg gctctgtttt tcgagcgcga aaaaaatccc
     1141 cgcaaccgaa atcgcgggga tttttcatgt ctgtcagccg gagccttcag attagttctt
     1201 gcgaaccagc agttccttaa tacgagcggc cttgccggag cggtcacgca ggtagtagag
     1261 cttggaacgg cagacatcgc cgtagcgctt cacttcgatg gaagcgatcg tcggggcgta
     1321 gagctggaac gtacgttcaa cgccttcgcc ggaagagatc ttgcggacga tgaaggaaga
     1381 gttgagaccg cggttgcggc gggcgatcac gacgccttcg aaggcctgaa cgcgcttgcg
     1441 ggaaccttca acgacgttga cgttcacgac aaccgtgtca ccggcgcgga aagaggggat
     1501 cgtcttgcca gcggtgacgc gggccatttc ttcctgttcg agaatctgaa tcagattcat
     1561 tttttttctc caaaaccatc gtcatgcggc tgtttttgag ggagctgagc ccaccgccga
     1621 gaggatggca aaattaatcc aaaatcttac ttccagccgt tggcgcgcaa ccacttgcgg
     1681 tccgcatcgc tcaacttgcc agaagaatcc gcgattttaa tcaagtccgg gcgagtttgc
     1741 aaggtgaatc gcaaactttc ttcgcgcgtc cacctgctga tgtttgcgtg gtgcccggaa
     1801 aggagcacgg agggcacctc catgccgcgc cagacttcgg gacgcgtata gtggggcgcg
     1861 tcaagaagcc cagtcgcaaa gctctcgtcc gacgtggaga gatccttgat ggcgccgggg
     1921 agctgtcgca ctacggcgtc catcagggcg catgcaggga tctcgccgcc cgagagaacg
     1981 aagtccccaa ggctcaccac ctcgtcgaca taggtcgaca ggaagcgctc gtcaatgccc
     2041 tcatagcgtc cgcacacgag cacgacactc tcatcctcgg ccaccatgcg gcgaacgcgc
     2101 tcatcggtga gcttcgtgcc gttgggtgca aaggagatga cgcggccgcg gttgccggat
     2161 gcacggatca tgtcgaccgt cttcgcgagc ggatcggcca tcatcaccat gccgggaccg
     2221 ccgccgaagg ggcggtcgtc gaccgtgcgg tgcacgtcct cggtcatggc gcggggattc
     2281 caggtgtggc aggaccacag gcctcgcttc atcgcacggg cggtgatgcc gcacttcgtg
     2341 atcgagtcga atatttccgg aaagagcgta acgacgtcaa agcgcatgat ccgcctcctc
     2401 agcgccagtc gggctgccag tcgacggtga tcgtgcggac gtcggtatca atggcgatca
     2461 gatagacgtc cttcacgagc ggaagcatga acacggcctt cttgccgtca gcattgtcgt
     2521 aggcgatgac gagcagatcc tgcgcgccgt tggagccgat gctctcgacc gtgccgaggg
     2581 ccgtgccctc cttgttgacg accttgcagc cgaccagatc gacggcccac acggcgtcct
     2641 tgcccgcatc aggaaagtcc gcgcgggaga ccgacacgcg gcctcgggcg gcgtcggccg
     2701 cctccttcgt ttcacagccc gcccacttgg cgatgaagcc gtctccgtga cgacggaagg
     2761 cctcgatgct gagcgtcgtg cgcacaccct tgaggtccgt gaggacccag gtcctggcct
     2821 tctgaagcac ttcgccggat tccagcggca caatgcgcac ccagccgcgg aagccgtagg
     2881 cgccggcggt gcgtccgagc tcgacgagat cgtccataac aacagtctcc gaaaatcaat
     2941 cgttttttgc gaacaaaaca aaaccccgca gactctcagg cgtagatacc gggctccagg
     3001 tgctgcgaac ggatcacgat gtgctggaca cgagaggcac gaccgcttgc gagcgagccc
     3061 gcacttgagg ttttgcgggg tttccgcgcc gtttaaagtc cggcgcaatg actagtgctt
     3121 tccgtcagga aattaggcgg cagccttggc ttccttcacg agacgggcaa ccgtttcgga
     3181 gagctgggcg ccctggctct gccagtaggc cagacgttcc tggttgacgt tgagacggac
     3241 ggcctgaccg gcagccatcg ggttgtagta gccgatgcgt tcgatgaagc ggccgtcgcg
     3301 gcggcagcgg ctgtcgcaga cgttgatgct gtagaacgga cgcttcttgg agccaccgcg
     3361 ggcgagacga acgataacca tgagtttacc tctttaactt attggttcaa ttgaaagccg
     3421 aagcccccgt cacgaggagc gttcggaaga attcttcatg cagcgcgcgg tcaatccgcc
     3481 cgttgcgaaa ggtctgaatt ttatctcacg ggaatacagg acgcaagtcc gtcaacgcga
     3541 ttttttcagc cttttctaaa aagaagcagc ccgcgcaagg cgggcgggca tgcaagcggg
     3601 gaaactccct cttcttactt ggtgccgagt ctggcgcgca aagcctcaag cgcctcgacg
     3661 ccgtcggcga cgagaccgta atcggccacc tcgaagatcg gagcctcggc gtccttgttg
     3721 atcgccacga ccgtcttggc atccttgatg ccagcggtat gctgaatggc accggagatg
     3781 cccacggcaa tatagagctc tggggcgatc atcttgcccg tctggcccac ctgccagtca
     3841 ttggggcaga gccccatgtc gacggcggtg cgggtggcgc cgacggccgc gccgatggag
     3901 tcggcgaagg cctcaagggt cttgaagccg gcctcgtcga gaagaccgcg cccgccgccg
     3961 acgacgatgc gggcggagac gagttcggga cggtcggact tggcttcgga gaagcgcacg
     4021 aagcgggagg cgtcatagcc ttccggagcg atgacctcct cgacgacagc ggatccctcc
     4081 tgagcggcgg cctcgaaggc cgtcgtgcgg atcgtggcca cgacgacggg atcggtcacc
     4141 tcgacggtcg cgagaagcga acccgcatag atgccgcgca cgaaggtttt ggggcccttg
     4201 acgtcgagaa cgtcggaaac gggggagacg tcgagcttcg cggccacgcg aggcatggcg
     4261 gacttgccga cggcggaggc gacgaagagc acgtgcgaat agccttcggc ttcgtcaagc
     4321 acgaggcgtg cgagcacttc gggcgactcc tgggcgagct ggggatggtc ggcgtagtag
     4381 acggtgcgca cgcccgagag cttcgcggcc tgctcggcaa cggactggcc gttgctgccc
     4441 gccacgagga cgtcgacgac gtccgtcatc ctgagcgcag ccgtgagcgc ctgagccgtt
     4501 gcggacttga gcgtcacgtt gtcatgttcg gcaatcaaaa gagctgtcat tttgctttac
     4561 tccctctttt ctcagatgac gtgcgcttcg ttcttgaggc ggtcgacgag ctcgtcgacg
     4621 ccggagacca tgacgcctgc cttgcgggcg ggcggctcct cgacgccgag gatgcggatg
     4681 cgcggagccg tgtcaatgcc gaggccggca agctcaatgt cctcgacagg cttcttgcgg
     4741 gccttgccca tggcgggcag cgtcacgtag cgcggctcgg caagacgcag gtccgccgtc
     4801 acgacggcag gcgttgcaag cgagacggtc tgcgtcccgc cgtcggtctc gcgggtaacg
     4861 acccagcggc cgccctcaag gacgatctcg ttggcaaagg ttgccagcgg catgtcgcaa
     4921 agggcggaga gcatctgccc cgtctggttg gcgtcgtcat cgatcgcctg cttgcccaga
     4981 agcaccagat cgggcttttc acgctcgacg acggcgcgaa gcaccttcgc gacagcgagg
     5041 ggctcgaggg actcgtcggt gcggacgtga acggcgcggt ccgcgccgat cgccatggcg
     5101 acgcgcaggg tgtcgacggc cttgagggga ccgacggtca cggcaacgac ttcgtcggcg
     5161 acgccagcct cgcgaagcgt gacggccttt tcgattgcga tttcatcaaa ggggttcatc
     5221 gccattttgg cgagcttgac atccacaccg gaatgatcgg gaagcgggcg caccttgatc
     5281 ttggcgtcca ccacgcgctt gacagcgacc aggatcttca tcaaaaaact ccagtcctat
     5341 tgagagggcg cgccttcaac ctgaagcgcg cgccgtccat gaaaaatcaa cttgcgggaa
     5401 attataacgg gactcaacgc ccgtttcgct taaggaaaag gccgttttgc ccacggaaac
     5461 acaggttccg caatgccctc agcccgcgct cagcggagcg agccgaaggc gtccgaaccg
     5521 cccgcaggcg ccttgaaacg ggaggcgacg gcaaaggcag ccgagacgcc catgcctgca
     5581 aaagtccaca cctcgtcccc cgtgacgttg cccaccaggc cgccgatgcc gccgaagacg
     5641 gcgcccgccg tccagagctg tgcgttgagg cgcctcaccc gcccgatgag cgcaatgaga
     5701 agcggcaggg ccactcccgg cacgaagaaa gagtaggctg tgagcagcag ccagatgatg
     5761 tccttcgaga catagacggc cgcagcggcg gcaaatccga agacgcccac gaagaagcgc
     5821 acggcgttcg tacggtctcc cgccagaagg cttcgctcga caatgccgga ggccgaaaga
     5881 agcaccgtgt cggccgaccc gcagagcgcg ctgatgaggc cgagcgcgag cgctcccttg
     5941 agccaggcgg gcatctccga agcactcgag agccagcccg ccacgggctg gggatcctgc
     6001 agattgagaa gcgcgagccc cgtcacgacg acgccgaacc acacgaggca gggcgaggcg
     6061 atccaggcgg cgaaacgagc ggactgtccg tctctggccg tgaaggtgcg tgagaacatg
     6121 tcgggaccta cgacataggt gatgcccacg agaagcatca tcctgcccag atcccacggc
     6181 ccgaacctgt cgcccaaggg gacgggatca agcgccatta cggcgtcggg acgatcgccg
     6241 agcaaccaga aggcggcggc cgtgaatccg cccagaagca gaacggcctg aaaggcgtcg
     6301 gtgcggatca ctgcactctg tccgcccgtg atggtatgca cgatcacaac gcccgcgatc
     6361 atcacataga gcacctcggc ggcgagcggg ctcgagagcg tcagcaggag cgtgtgaagc
     6421 gcgacgaact gcgcggccgt gacggcgatc caggagaccg caatgatgac gcccgcccag
     6481 cgctcagcat tcctgcccgc cacgacgccg accacctcgg gaagcgtaac ggcgggaagc
     6541 gcacgaatct tcggagcgat ccatccgtga agaagaaggc cgaccgcacc tacgccgagc
     6601 caccagaagg cgcccgaacc aatcttctgc gcgaggcttc ccatgccgag cgttgcggac
     6661 ccgccaatga tggtgctcac gagagagagc gcgcagagaa gcgcgcctgc tcggccgccg
     6721 ctcgtaagaa aggcgcgccc cgagctcgaa tgtcgctccc cgcagaagcc gaaccacatc
     6781 agaacgatca gataaacgag cacaaggcct gtaagcatgt tcacgacgat ttcttccttt
     6841 ccattgtttc aggcagaccg actcagacaa gcgagagcgt gcccgccgcc acgacgacgt
     6901 agcgcagaag ctttcccacg gccacccaga ctgcacagct tgcagcaggc atcctgagcc
     6961 agcccgccgc aagcggcagg gcgtctccca cgacgggcag ccacgacaga agcagcgtgg
     7021 gcgctcccca tcgcctgagc cacacaagcg ccctgtccgt cttctccctt tcgggaatca
     7081 gtcgaccgat gagccacgtg gtcatcgcgc cgagcgtatt gcctgccgtt gcaaggagca
     7141 cgagacgaca gaggcggggc gtgtcgtcag gcgtttgcga cagcgcccct gccaggagcg
     7201 cctcggatcc gccaggcatc agcgtcgagg aaatgaaggc actcgcgaaa accgcagcct
     7261 cggcccctgc gcccgacagc cattcaaaaa gaaagtcctg catcggacgc ggctccttcg
     7321 tgggaattca aaaaacgaaa cgccgccccc aacaggagag cgacgttccg attgtagcct
     7381 gatcagtccc tgctcaggta cagggtcaga gacggatgcg cctcgtcgtt tcaggatcga
     7441 accagtacac gtgttccgcc gagaactcga gtcccgccct gatgcccact tccgggatgt
     7501 acatgtggtt gtcgacgcgg aagaccacgt tctgccccag caggacgccg tggatcagat
     7561 gatcggcgcc gagggcctcg accgtctcca cctcgaaggc ggcgagtccg ccctcgggac
     7621 ggagcgtcac gtgttcgggc cgcacgccga gcaccaccgc cctgccggca agtgccgtgc
     7681 agggttcggg cagcgacagg acacccgaat cgtgctcgaa gtgactgccg tctgcggaga
     7741 cgcggccgtg aacaaggttc atcggaggag agccgataaa gttcgcgacg aacatcgtct
     7801 cgggatcgtc atagacctcc tgaggcgtgc caacctgctc ggcgcggcct gcgttcatca
     7861 cgatcatgcg gtgggcgagc gtcatggcct cgacctgatc gtgagtgacg taaagactcg
     7921 tcgtcttgag acggcggtgc agcttctgga tctgcaggcg cgtctgcacg cgcagctttg
     7981 cgtcgaggtt cgagagcggt tcgtcgaaga gaaagacgga gggctcgcgc acgatcgcgc
     8041 ggcccatggc gacgcgttgg cgctggccgc cggagagctg gcgaggacgg cgcgcaagca
     8101 ggcctccgag ttcgagcatc ttcgcggcct cgttgacgcg acgctcgatc tcgtccttgg
     8161 gaaccccgcg gatcttgagg ccgtaagcca tgttgtcgaa gaccgtcatg tgcgggtaga
     8221 gcgcatagtt ctggaacacc atggcgcagt cgcggtcctt gggttcgaga ttgttgacga
     8281 cgcgctcgcc gatgatgagt tcgcccgagg tgatctgctc gaggcccgcc accatgcgca
     8341 gcagggtcga cttgccgcag cccgacgggc ccacgatcac gatgaactcg ccgtccctga
     8401 tgtcgagcga gatgccgtgc acgactgcca cgtcgccgta gctctttcgg atgtttttga
     8461 attgaatgtt agccatgttg tctgtctctt acttttccgt gtcgacgagg cccttgacga
     8521 accagcgctg catgacggtc accacgatgg cgggcgggat cattgcaagc atggccgtcg
     8581 ccatgatgag gttccattcg ttggcggagt cgccgccgcc gatcatgcgc ttgatgccga
     8641 tcacgatcgg gtacatgtcc tcggacgtgg tcacaagcag cggccacagg tactggttcc
     8701 agccgtagat gaactgaatc acgaacattg ccgcgatgtt ggtgagggaa agcggcatca
     8761 gaatcgtgaa gaagaagcgc atcggcccgg cgccgtccat gcgggcggct tcggtgagct
     8821 cgtcgggaac ggtgaggaag aactgcctga agaggaaggt cgccgtcgct gacgcgatca
     8881 cgggaagaat caggccgccc cagctgtcaa gaaggtgcag atccgccatc acttcatagg
     8941 taggcgagat gcggacctcg acgggcagca tcagcgtgat gaagatgagc cagaagcaga
     9001 gcttgcggcc ggggaacctg aagtaaacga tcgcgaaggc cgagacgagc gagatcagga
     9061 tcttgccgat cgagatgccc atggccatca tgagcgagac gaagagcatg cggcccacgg
     9121 gggcgcgcga agcggtgccg ccgccctcag tgaggatctg catgtagttt tcgatgaagt
     9181 gggcgccggg aagaaggctc atgggcgcct gttccacttc gacggcggtc tgtgtcgacg
     9241 cgacgaacgc cacgtagacg gggaagacca ccacgatcac gcccagaatc agaatcaggt
     9301 gcgtgaagaa attgagccag ggacgatttt caaccatctg tcgtctcccg tcagtagttg
     9361 acgcgccgct cgatgaagcg gaactggatc accgtgagga tgatcaccat caccatcagg
     9421 atgacggact gtgcggcgga gccgccgaag tccatgttgc ggaagccgtc gacgaacacc
     9481 ttgtagacga gaatgttcgt gtcctgcccc ggaccgccca tggtcgtcgc atcgacgatg
     9541 gcgaacgtgt cgaagaacgc gtagacgatg ttcatcacga gcaggaagaa ggtcgtgggc
     9601 gacagcagcg ggaagaccac cgtccagaag cggcggaagg gacccgcgcc gtccatggcc
     9661 gccgcctcga gcagcgactt cggaatggcc tgcagacctg cgaggaagaa gatgaagttg
     9721 tagctcacct gcttccagac cgccgcgatg acgagaagcg tcagggcctg gtccgcgtcg
     9781 accagatagt tccagtcgat gccgaacatg cgcatcacgt aggagagaac gccgagcgtc
     9841 ggattgaaga tgaacatcca gaggacgccc gccacggcgg gggcgacggc gtacggcacg
     9901 atgaggaagg tcttgtaggc cgaggcgccg cgcagcacgc ggtccgcaca gacggcaagc
     9961 agaaggctga tcgcaaggcc gaggaaggcc acgagcgccg agaagaccat cgtgatcttg
    10021 aagctcgaga catagttcgg gtcggacaga agcgtttcaa agttctgaag acccacgaat
    10081 tccgtgctga gcccgaaggg atcctgcatc agcaccgact ggtagatggc ctggccggcg
    10141 ggcaagaaga agaagatgag cgtaatggcg agctgcggcg ccaccagcag gtagggcagc
    10201 cagcggtttt ggaatacgac tcgtttttcc acgttgttga ctctgaggat attaaaaacc
    10261 taaccgcaag catcccgcaa gacggcaggg ccgtgtgccc tgacgtcttc gggatgcggt
    10321 atcgcagcag gacggacgcc ttcaggggtt cgagggcgtc cgcgagctgt cgtcggaaag
    10381 ggctcgtgct tacttcgtca cgcgttcaaa gcggacgagc tgctcgttgc cgcggcggac
    10441 gacggcatca agggcctcct tggaggtcgc cttgccggac cagatctttt cgaattcctc
    10501 gtcaacgatc gtgcggatgt tgggcaggta gccgagacga atgccgcggc tcttgtcggt
    10561 cgtcttgagc atctgcttga cggcgacgtc tgcgcccgga ttcttttcgt agtagccgct
    10621 ggccttggcg atttcaaagg ccttctgcgt caccgggaga tagcccgtgc gcatgtggtt
    10681 gttcgcctga acctgcgggt cggagaggta gtggaagaac tcggcgacgc agcggttctg
    10741 ctcgtcggac ttgcctgcca tggcccagag cgaggcgccg ccgatagcgg tgttctgcgg
    10801 agcatcaggc acgccctcgt agtagggcat cggcatcgtc atgtactcga acttcgcgtt
    10861 gcggcggatg ttgccgagcg agcccgagga gcccatgttg atcgcgcatt cgcccgagta
    10921 gaaggtggcg tcggcaagat tgccgcggcc cttgtagacg aaggtgcctt ccttggccat
    10981 gcgggccaga ttttcaagat ggcgctgatg gagcggagag ttgaccatga ggcgcgcgcc
    11041 cgtgccgccg aagccgttgt tgagcgtggc gtactcgacg ttgtgccagg tggagaagct
    11101 ctcgagctgg gtccagccca tccaggaagt ggtcatcggg caggtttcgc ccgcggcctt
    11161 gagggccttg gcggcctttt caatctcagg ccaggtgcgc gggagcttgt ccggctcggt
    11221 cggcaggcct gcgttcttga acttggtgag attgaggtag agaacggtgg tggaggagtt
    11281 gaacggatag ctgagcatcc tgccgtcggc cgtggaatag tagccgtaga cggcagggat
    11341 gtagtccttg ggattgaatt cgtagcctgc cttgtcgaga agctcgccga caggaacaat
    11401 ggcacccttg ctgaacatca tggttgccgt gccgacttca aacacctgaa gaatgttcgg
    11461 ggagcgtcct gcgcggtggg ctgcgatgcc cgacgtcatc gtctggtcgt aggtgccctt
    11521 gtacgtggac gtgagatgac actgcggggt cttggcgttg aagtccttgg tcacgtcggc
    11581 aacccagtcg ccgagcgtat tggccatcgc atgccacatc gtgatttcag tgggagcggc
    11641 ggaggccgcg cccgcggcca taagggaagc cgccatcacg ccggcagcga caaacttttt
    11701 gctcatgctg ttctcctttg agcagagggg gcctttgcca ggcgccgtca gcagcatccg
    11761 tcaccggtct ccgagggcac ttccatgaag gaggaaaggc aaactccttg gacgaaaagc
    11821 cttcgtccga ttttggatca taggggatgc cccctcccct gtccatacgc cgatcggtgg
    11881 atgcgcctac caccagatgc tgaagccgcc tccaaagtgc gctctggcag gccttcgcgg
    11941 catcatgatg ggattttccc tacgaaaggc gttttctgca tccaactgcc tcaaacggcg
    12001 cacttcgccg aatagtcgcg cggtccgaag acggccctgc cgaccctcac catggtcgcg
    12061 ccctccgaca ccgcctcgtc catgtcggcg ctcatgccca tggaaagcgt gtccatgccg
    12121 gggtgcttca cacggagccg ctcgaagagt gcgcgcatgg
//
LOCUS       DBMDTI010000036        11776 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1641_length_11776_cov_4.327024, whole genome shotgun sequence.
ACCESSION   DBMDTI010000036 DBMDTI010000000
VERSION     DBMDTI010000036.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11776)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11776
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1641_length_11776_cov_4.327024"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="ACZPB6_03275"
     CDS             complement(<1..357)
                     /locus_tag="ACZPB6_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR1183527.1"
                     /translation="MAILSNTEFGILRLTISRAQKRNSINAEMFDTMTEALSRAASDD
                     AVRVVLLQGGEQIFSAGADLEEMRSQPEVLDHAMTNFFAALRAFPKPVVAQVTGPCVG
                     DALSMLLYCDLVYASER"
     gene            complement(398..3097)
                     /locus_tag="ACZPB6_03280"
     CDS             complement(398..3097)
                     /locus_tag="ACZPB6_03280"
                     /EC_number="2.3.1.-"
                     /EC_number="6.2.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF024938.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0046356 - acetyl-CoA catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HIR1183528.1"
                     /translation="MNPIRPTLKRALCPENIAVIGASDRTASRGSFVWRAVARSKLLK
                     NAWAINPKYKYIGERPCFSSINEVPGSIDLAVISLRADRILKALIDAGERGVAYALIT
                     PDENAYASDATWLTKLQLVADQYGMRLIGPDSLGLMNPGLGVNASYWPNLPRPGNIAL
                     ITQSGMIGTALLDYAQGAELGFSGVVSTGAAIDVDMPELIDYYANDKNTRVIALHIEG
                     IRRPREFYSSLRAACARKHVVILKAGSGSGYAADRIACFKMGTDAGSEGALAALVERA
                     GATLVPTFEEFTAAVSGFATNRLPRGNRIAVIANGSGFASLTASAAQACGIDLHGLSN
                     ATIKDLKTAYPSQQIAVNPVNVGATASPERYRKTLQIVLQDPMIDGALVVVAPSPVTT
                     IDPTLKLLAKTAAGSYKPVITAWVSDRIALSVRKQLRSVPNAPISAIQSPVAAALAFG
                     YLAAEARRRADRLSIPPEWSVELNPAKLAEARAIMNGARLEGRHALSPSETGRLLACF
                     EIPVVPLREVGTLEEAGAAADELAWPVALKVTAPGLRHKSDIGGAVLDIKDRDGLAAA
                     WRSMEESIVNNSPMTPMVGAVVQKMVPHSSVRELHLAIAYDNVLGPVVEFGAGGIGSE
                     LYQDKKAALVPINLSEADRLVESTRIARALGTYRGLAPADRTLIAQMLCRLSVLAALI
                     PAIRDLTINPLVWTDGGAAAIDADVNLNEAPVESEPGYPHLTVCAPPWEEGREVVFGG
                     EALTLRTFVQSDFHPLKRFLGRLSEKTFYLRFHTSVHLSDERICELASLDYAREAAWA
                     LAKDGEIHAVARWRLTGELGEAEFGIVVEDAWQRRGLARVLMAHIETTASERGVTRLV
                     GHVLKGNEAMQGMMTAMGYTLGEGDSRDTDPWVKDISIPTNLL"
     gene            3260..4348
                     /gene="mtnA"
                     /locus_tag="ACZPB6_03285"
     CDS             3260..4348
                     /gene="mtnA"
                     /locus_tag="ACZPB6_03285"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR1183529.1"
                     /translation="MKVYGEPTRSIRSLDNGTAVEIVDQTLLPYRFEKRVLGDWRDCV
                     EAIRTMRVRGAPLIGITGAWAVVLAVKENPENDALLKAAEVIANARPTAVNLSWAVRR
                     MMSTLLPRDPALRFEAAVKTAEAMTQEDVMTCRAIGEAGMTLISDLYARLRRPVNVLT
                     HCNAGWLATVDYGTALSPIYMAFEQGTPLHVWVDETRPRNQGLLTAWELGQHGVPYTL
                     VADNAGGQLMQQCDVDLVIVGADRITRRGDVANKIGTYLKALAAVDNEVPFYVAAPSS
                     TIDWTLNDGKREIPIENRAASELLVVNGVGSDGETRTVQIAAPEVAVANPAFDVTHNR
                     FVTGIITERGIVKPTDLTAVFADLLPKA"
     gene            4353..5045
                     /locus_tag="ACZPB6_03290"
     CDS             4353..5045
                     /locus_tag="ACZPB6_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR1183530.1"
                     /translation="MMQDTTALRAELIETARGMNLRGINVNKSGNVSARACRADGAEG
                     FLITPTGMPYESLTPDDLVWIPLVNPPSLRNADGARRPSSEWEMHARVYLRRSDIDAV
                     VHTHSCFATALACQDMPIPPFHYMVAAAGGHSIDVAPYATFGTPELARVAAEALAVKN
                     ACLLSHHGVLAAGKSLQAALTLAVEVENLAHQYVVVCQLGKPKLISYEEMNEVLRSFR
                     TYGQQSVEGESK"
     gene            5042..5296
                     /locus_tag="ACZPB6_03295"
     CDS             5042..5296
                     /locus_tag="ACZPB6_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183531.1"
                     /translation="MTTKAPERWLRVDGSVVSCTESVKVLNENWHEARDMLQEMFEDA
                     VLLGCGKEAFRAEFRRLIDGLDCDYKELLQPVEAKGVKGD"
     gene            5299..5808
                     /gene="bamE"
                     /locus_tag="ACZPB6_03300"
     CDS             5299..5808
                     /gene="bamE"
                     /locus_tag="ACZPB6_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE
                     domain-containing protein"
                     /protein_id="HIR1183532.1"
                     /translation="MRIRALGCALASAACLLLTACSHPQLMDPGMTESQVRTELGAPH
                     AAVDTGKGTQRWVYSMQPFGQEVWWIEFDADGRIVRREEVLDRAHMALIKPGVSTEKD
                     VWNLFGKCAQEYTFSLIDQTAWMYRFKDDGLFDMACWVQFDVNGVVTEVGFTTDPWKD
                     RDGLFWGFN"
     gene            5818..6858
                     /locus_tag="ACZPB6_03305"
     CDS             5818..6858
                     /locus_tag="ACZPB6_03305"
                     /inference="COORDINATES: protein motif:HMM:NF048615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7146 domain-containing protein"
                     /protein_id="HIR1183533.1"
                     /translation="MRDPQFMARVQRVQEAASGRWLDILIDAGLPEVMLLRLNRPCPL
                     CGGRDRFSFYRNKPDGHWFCRGCGYGDGLSLLQKWRSERFTDTLVYLERVLGLPEYKR
                     QDTGPDSVQTAEAAAKKRRQDRIEAVWKEAQALSKVPPSDPVRLYLASRGLYCGSICE
                     LRSHPLLDYWEELDDGDRTHKRWPAMLALVTDDEGRIVTLHRTYLTPEGIKAPVEAPK
                     KLYAGGARGGLIRLGEPSDVLGIAEGIETALSARTLFKIPVWSAVSVGGFQNFTKVPD
                     GVRKAVVFGDNDRSYAGQAGAYALAARLRRDNPSLEIEVEIPKTEGLDWSDVWMRRRT
                     GSRRPSGAGTKA"
     gene            complement(6959..8770)
                     /gene="aspS"
                     /locus_tag="ACZPB6_03310"
     CDS             complement(6959..8770)
                     /gene="aspS"
                     /locus_tag="ACZPB6_03310"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR1183534.1"
                     /translation="MRTVYSGLVDESFIGQTVTLFGWAHRRRDHGGVIFIDLRDREGI
                     VQVVCDPDRPEVFATADKCRNEFCLRVVGVVRARPEGTKNETLVSGGIEVLCHELEIL
                     NPSVTPPFHLDDENLSETTRLTYRVLDLRRGVMQKNLRTRYRAAMAFRKFLDKNGFID
                     IETPMLTRSTPEGARDYLVPSRVQDGCFFALPQSPQLFKQLLMVAGFDRYYQIVKCFR
                     DEDLRADRQPEFTQVDIETSFLNMDEIRALMEELIRTVFKEVLDVDLKNPFPVMNWDD
                     AMAQYGSDKPDLRVNLKLVEVTDVVENSTFKVFSGVKSLHNGKVVALRVPGAASMPRS
                     EIDAYTEFVKIYGAKGLAYIKINDLSKGREGLQSPIVKNLSDEELNTIIERTGAQDGD
                     VVFFGADKAKIVWDSLGALRLKIGHSEFGKSHGLFTPGWQPLWVINFPMFEYSEEDQR
                     WVACHHPFTSPLDGHEDLLVSDPEHAYAKAYDMVLNGWEIGGGSIRIHREEVQEKVFE
                     ALKIGPEEARQKFGFLLDALQFGAPPHGGLAFGLDRIVTMMCEADSIRDVIAFPKTQR
                     AQCLLTHAPAPVDEKQLRELHIRLRNEQKKELEIAGQ"
     gene            complement(9102..9332)
                     /locus_tag="ACZPB6_03315"
     CDS             complement(9102..9332)
                     /locus_tag="ACZPB6_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR1183535.1"
                     /translation="MPIYAYRCTNCGFEKDFLQKMSDKPLTKCPQCGKDALVKELSAP
                     GFELRGTGWAATDFNGGHKALPASHRKPDSSK"
     gene            complement(9472..11523)
                     /gene="fusA"
                     /locus_tag="ACZPB6_03320"
     CDS             complement(9472..11523)
                     /gene="fusA"
                     /locus_tag="ACZPB6_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR1183536.1"
                     /translation="MSNYLTENLRTVALVGHGSCGKTSLIEAMLYRTGMISELGSIER
                     GNTMCDNDALEKQVQHSIRLAVAHVDTALPDLTPVRIHVLDTPGYPDYIGQDMSALDA
                     VKSVCAVIDATKGVELLTRRMMDIAKERGLCRMIVINKFDAPDVDLEQLLLDLQEVWG
                     PGVLPINLPANNRSIVIDCFDKDEGEADILSVGEVHRAFIEKIIEQDDEMLEHYLEEG
                     SVDPRTLHPLITKALREGTVIPVCFVSAKNLIGIRDFMKVIIRHLPSPAEANDALFIH
                     ADGTPFITRPEKDLPVVAQVFKVVNDPYIGKIGIFRVHQGTIQKDTTLYVDDNKKGVK
                     AAHPLLLQGKNTTETDFLSPGDIGALAKIDELTYGSILHSDPNLCDVRMRPINFPKPM
                     FGLAIAPSRRGDEARMGEVLNKMLSEDPTMAVDHDAVLNETVIRGLTEMHVRSILDRM
                     KVNFKLEVVTGTPSVPYRETITAPAEGHARHKKQTGGAGQFGEVYLRIEPLERGAGFE
                     FHDEVKGGVIPFNLIPAVEKGVREVLDSGYLAGYPIQDVKVTVYDGKSHPVDSKEVAF
                     VAAGRKAFLDALANAQPKILEPIVRIEITAPDMYMGDLAGDLASRRGQVSGTDSLPGG
                     LMEITGVVPLSELDGYATRLHALTQGTGSWSMELDSYQPIPAQKQAELAGKFHREDDE
                     E"
BASE COUNT         2110 a         3400 c         4078 g         2188 t
ORIGIN      
        1 gcgttcggat gcgtagacga ggtcgcagta gagtagcatc gagagcgcgt cgcccacgca
       61 cgggcccgtc acctgggcga cgacgggctt cgggaaggcg cgcagcgcgg cgaagaagtt
      121 cgtcatggca tggtcgagca cttcgggctg ggagcgcatc tcctcgagat cggcgccggc
      181 ggagaagatc tgctcgccgc cctgcaggag cacgacgcga acggcgtcgt cggaggcggc
      241 tcgggagagg gcctccgtca tggtgtcgaa catttcggcg ttgatgctgt tgcgcttctg
      301 agcgcggctg atcgtcaggc ggagaatgcc gaattcggtg ttggacagaa tggccattgc
      361 tgtgtttccc atgaagaaga atgtctgagg cgcgccatta taggagattc gtcgggatgg
      421 agatgtcctt cacccacgga tcggtgtcgc ggctgtcgcc ttcgccgagg gtgtagccca
      481 tggccgtcat catgccctgc atggcctcgt tgcccttgag gacgtggccg acaagtcggg
      541 tgacgccgcg ttcggacgcc gtggtctcga tgtgcgccat gagcacgcgt gcaaggccgc
      601 ggcgctgcca ggcgtcctcg accacgatgc cgaactcggc ttcgccgagc tcgcccgtca
      661 ggcgccagcg tgcgacggcg tggatttctc cgtccttggc gagcgcccag gcggcctcgc
      721 gggcgtagtc aagcgaggcg agctcgcaga tgcgctcgtc ggagaggtgg acggacgtgt
      781 gaaagcgcag gtagaaggtc ttctcggaca agcgtccgag gaagcgcttg agcgggtgga
      841 agtcgctctg cacgaaagtg cgcagcgtga gcgcctcgcc gccgaacacg acttcgcggc
      901 cttcctccca tggcggcgcg cagaccgtca ggtgcggata gccgggttcg gactcgacgg
      961 gcgcctcgtt cagattgacg tccgcatcga tcgcggcggc gccgccgtcg gtccagacga
     1021 gcgggttgat ggtgaggtcc ctgatggcgg ggatcagggc cgcgagcacg gagagtctgc
     1081 agagcatctg cgcgatgagc gtcctgtccg caggcgcaag ccccctgtag gtcccgagtg
     1141 cgcgtgcgat ccgggtgctc tccacgagac ggtcagcctc gctcaggttg atcggcacca
     1201 gcgcggcctt cttgtcctga taaagctcgg agccgatgcc gcctgcgccg aactcgacca
     1261 cgggaccgag cacattgtca taggcaatgg cgagatgaag ctcgcgcacg gacgagtgcg
     1321 gcaccatctt ctgcacgacg gcgcccacca tgggcgtcat cggggagttg ttgacgatgc
     1381 tttcctccat tgagcgccat gcggcggcga gaccgtcgcg gtccttgatg tcgagcaccg
     1441 cgcccccgat gtcggacttg tgcctgaggc cgggggcggt caccttcagg gccacgggcc
     1501 aggcgagttc gtctgcggcg gcgcccgcct cttcaagcgt cccgacctcc ctcagcggca
     1561 cgacgggaat ctcgaagcag gcgagaaggc gccccgtctc gctgggggag agcgcatgac
     1621 gcccctcaag gcgcgcgccg ttcatgatgg cgcgtgcctc ggcgagcttg gcggggttga
     1681 gttccaccga ccattcgggc ggaatgctca gcctgtcggc acgcctgcgg gcttcggccg
     1741 ccagatagcc gaaggcgagt gcggcggcca cgggcgactg gatggcggag atcggggcgt
     1801 tgggcacgga gcgcagttgc ttgcgcacgc tcagcgcgat cctgtcgctc acccaggcgg
     1861 tgatgacggg cttgtaggag cctgcggccg tcttggcgag aagcttgagc gtcgggtcga
     1921 tcgtggtgac ggggctcggc gccacgacga cgagcgcgcc gtcgatcatg gggtcctgaa
     1981 gcacgatctg cagcgtcttc cggtagcgct cgggcgaggc cgtggcgccc acgttgacgg
     2041 ggttcaccgc aatctgctgg gagggatagg cggtcttgag gtccttgatc gtggcgttgc
     2101 tcaggccgtg gagatcgatg ccgcaggcct gggcggcgga ggcggtgagc gatgcgaagc
     2161 ccgagccgtt ggcaatgacg gcgatgcggt tgccgcgggg aagacggttg gtcgcaaagc
     2221 ccgagacggc tgcggtgaac tcctcgaagg tgggaacgag cgttgctccc gcacgctcta
     2281 cgagcgctgc gagcgcgcct tcgcttcccg cgtcggtgcc catcttgaag caggcgatgc
     2341 ggtcggcggc gtagcccgag ccgctgcctg ccttgaggat gacgacgtgc ttgcgcgcac
     2401 aggcggctct gagggacgag tagaactcgc gcggacggcg gatgccctcg atgtgcaggg
     2461 cgatgacgcg cgtgttcttg tcgttcgcgt agtagtcgat cagttcaggc atgtcgacgt
     2521 cgatggcggc gcccgtcgag acgacgcccg aaaagccgag ctcggcgccc tgcgcgtagt
     2581 caagcagcgc cgtgccgatc atgcccgact gggtgatgag cgcgatgttg ccgggacggg
     2641 gcaggttggg ccagtagctt gcgttgacgc cgagccccgg gttcatgagc ccgagggagt
     2701 cgggaccgat caggcgcatg ccgtactggt cggccaccag ctggagcttc gtgagccagg
     2761 tcgcgtcgct cgcgtatgcg ttttcgtccg gcgtgatgag cgcgtaggcg acgccccgtt
     2821 cgcccgcgtc gatgagcgcc ttcagaatgc ggtcggcgcg cagggagatc accgccagat
     2881 cgatgctgcc cggcacctcg ttgatgcttg agaagcaggg gcgctcgccg atgtacttgt
     2941 acttggggtt gatcgcccag gcgttcttga ggagcttcga tcgggcgacc gcgcgccaga
     3001 cgaaggagcc gcggctggcg gttctgtcgc ttgcgccgat gacggcgatg ttttcaggac
     3061 agagggctcg cttgagcgtg gggcggatgg ggttcaaggt gacctcccag gcggtggttg
     3121 atccgtatgc gtttgcgttc aattttgcta cgaaccggcg ggtgaaggca aaggaggctg
     3181 ctgatgcgaa attcggtaca ctaggaatca gggcgaggtg cgttccgacc cgccagtccc
     3241 gtcagaaagg tgaagacata tgaaagttta cggtgaacct acaagaagca tccgctccct
     3301 tgacaacggc acggccgtcg agatcgtcga tcagacgctc ctgccgtatc gctttgaaaa
     3361 gcgggtgctg ggcgactggc gggactgcgt ggaggccatc cgcaccatgc gcgtccgcgg
     3421 cgctccgctc atcggcatca ccggtgcgtg ggccgtcgtg ctcgccgtca aggagaatcc
     3481 cgagaacgac gccctgctca aggccgccga agtcatcgcc aatgcgcgtc caacggccgt
     3541 gaacctttcc tgggccgtgc gcaggatgat gagcacgctt ctgccgcgcg accccgcgct
     3601 tcgcttcgag gccgccgtga agacggccga ggcgatgacg caggaggacg tgatgacctg
     3661 ccgcgccatc ggcgaggcgg gcatgacgct catctctgac ctctacgcca ggctgcgccg
     3721 tcccgtgaac gtgctcacgc actgcaacgc gggctggctt gccaccgtcg actacggcac
     3781 ggcgctctcg ccgatctaca tggcgttcga gcagggcaca ccgctgcacg tctgggtcga
     3841 tgaaacccgt ccccgcaacc agggccttct cacggcctgg gagctcggcc agcacggcgt
     3901 gccctacacg ctcgtcgccg acaatgcggg cggccagctc atgcaacagt gcgacgtcga
     3961 tctcgtgatc gtgggcgccg accgcattac gcgtcgcggc gacgtggcca acaagatcgg
     4021 cacctacctc aaggcgcttg ccgccgtcga caatgaagtc cccttctacg tggccgcgcc
     4081 atcctcgacc atcgactgga cgctcaacga cggaaagcgc gagatcccca ttgaaaaccg
     4141 cgccgccagc gagcttctcg tggtcaacgg cgtgggcagc gacggcgaaa cgcgcaccgt
     4201 ccagatcgcc gcacccgaag tcgccgtggc caaccccgcg ttcgacgtca cgcacaaccg
     4261 cttcgtgacg ggcatcatca ccgagcgcgg catcgtgaag ccgacggatc tgaccgcggt
     4321 atttgccgac ttattgccaa aggcctgaag aaatgatgca ggatacgacg gcgctgcgcg
     4381 ccgaattgat tgaaaccgcc cgcgggatga atctgcgcgg gatcaacgtg aacaagtcgg
     4441 ggaacgtctc ggcgcgcgcc tgcagggccg acggtgccga gggcttcctg atcacgccga
     4501 cgggcatgcc gtacgagtcg ctcacgcccg acgatctggt ctggattcct ctggtgaatc
     4561 cgccttcgct gcgcaatgcc gacggcgcac ggcgcccgtc ctccgagtgg gagatgcatg
     4621 cgcgcgtgta tctgcgccgc tccgacatcg atgcggtcgt gcacacgcac tcgtgctttg
     4681 cgacggcgct tgcctgtcag gacatgccga ttccgccctt ccactacatg gtggctgcgg
     4741 caggcggcca ttcgatcgac gtggcgccct acgcgacctt cggtacgccc gagcttgccc
     4801 gcgtcgccgc cgaggcgctc gccgtcaaga acgcctgcct gctttcgcac cacggcgtgc
     4861 tcgctgcagg caagtcgctt caggcggcgc tcacgctggc ggtcgaggtc gagaacctgg
     4921 cccatcagta cgtcgtcgtc tgtcagctcg gcaagccgaa gctcatttcc tacgaggaga
     4981 tgaacgaagt gctgaggagc ttcaggacct acgggcaaca gtccgttgag ggagagtcca
     5041 agtgacgacg aaggcgcccg aaaggtggct cagggtcgac ggctccgtcg tctcctgcac
     5101 ggagagcgtc aaggtgctca atgaaaactg gcacgaggcc cgcgacatgc ttcaggagat
     5161 gttcgaggac gccgtgcttc tcggttgcgg caaggaggcc ttcagggcgg agttccgccg
     5221 tctgatcgac ggccttgact gcgactacaa ggagctcctt cagcccgtcg aggccaaggg
     5281 agtcaagggg gactgaccat gagaatcaga gcactgggct gcgcgttggc atcagcggcc
     5341 tgtcttcttc tgacggcctg cagccatcct cagctcatgg atcccggcat gaccgagtca
     5401 caggtcagga cggagctcgg cgcgccgcac gcggccgttg acacgggcaa gggcacgcag
     5461 cgctgggtct attccatgca gcccttcggt caggaggtct ggtggatcga gttcgatgcg
     5521 gacggcagga tcgtgcggcg cgaggaggtg ctcgacaggg cgcacatggc actcatcaag
     5581 ccgggcgtga gcacggagaa ggacgtctgg aaccttttcg gcaagtgtgc ccaggagtac
     5641 accttttcgc tcattgacca gacggcctgg atgtaccgat tcaaggacga cggtcttttc
     5701 gacatggcct gctgggttca gttcgacgtc aacggcgtcg tgaccgaggt gggcttcacg
     5761 accgatccct ggaaggacag ggatggcctt ttctggggat tcaactagga ggcggacatg
     5821 cgggacccgc agttcatggc gagagtgcag cgcgttcagg aggcggcctc cggccgctgg
     5881 ctggacatac tgatcgacgc aggattgccc gaggtcatgc ttcttcgcct gaacaggccg
     5941 tgtccgctct gcggcggcag ggaccgcttc tccttctaca ggaacaagcc cgacggccac
     6001 tggttctgca ggggatgcgg ctacggcgac ggactgagcc ttctgcagaa gtggcgcagc
     6061 gagcgcttca cggacacgct cgtctatctc gagagggttc tcggacttcc tgaatacaag
     6121 cggcaggaca ctgggcccga ctccgtgcag actgcggagg cggccgcgaa aaagcgccgt
     6181 caggacagga tcgaggcggt ctggaaggag gcgcaggcgc tctccaaggt cccgccgagc
     6241 gatcccgtca ggctttatct tgcgtcccgc ggcctttact gcggctcgat ctgcgagctt
     6301 cgcagtcatc cgcttctgga ctactgggag gagctcgatg acggagatag aacgcataag
     6361 cgatggcccg cgatgctcgc gctcgtcacc gacgacgagg gacggatcgt cacgctgcac
     6421 aggacctatc tgacgcccga gggcatcaag gcgcccgtcg aggctcccaa gaagctttat
     6481 gcgggcggcg cccgtggcgg cctcatcagg ctcggcgaac cctcagacgt gctcggcatt
     6541 gccgagggga ttgaaacggc gctgtcggcg aggacgctct tcaagattcc cgtctggagc
     6601 gccgtgagcg tcggcggatt tcagaatttc acgaaggtgc ccgacggggt gaggaaggcc
     6661 gtcgtcttcg gagacaacga caggagctat gcggggcagg cgggcgcgta tgcgctggcc
     6721 gccagactgc gcagggacaa tccctccctt gagatcgagg tcgagattcc caagactgaa
     6781 ggactggact ggagcgacgt gtggatgcgg cgcaggacgg gcagccgccg tccctcgggg
     6841 gccgggacga aggcctgagg gcggggcgct ccgcacctcc gtggaccatg aacaagaagg
     6901 gccgcttccg tgaggaagcg gcccttctgt gtcagaaccg aatgttcagg tcagcggatt
     6961 actggccggc gatttcgagt tccttcttct gctcgttgcg caggcggatg tggagctcgc
     7021 gcagctgctt ttcgtcgacc ggagccggag cgtgcgtcag caggcactgg gcgcgctgcg
     7081 tcttcgggaa ggcgatgacg tcgcggatgg agtccgcttc gcacatcatc gtgacgatgc
     7141 ggtcgagacc gaaggcgagg ccgccgtgcg gaggagcacc gaactggagg gcgtcgagca
     7201 ggaagccgaa cttctgacgg gcttcttccg gaccgatctt gagggcttcg aagacctttt
     7261 cctggacttc ttcgcggtgg atacggatgg agccgccgcc gatttcccaa ccgttgagga
     7321 ccatgtcgta ggccttggcg taggcatgtt ccggatcgga gacgaggagg tcctcatgac
     7381 cgtcgagcgg gctggtgaac ggatggtggc aggcaaccca gcgctggtct tcttccgagt
     7441 actcgaacat cgggaagttg atgacccaga gcggctgcca gcccggggtg aagaggccgt
     7501 gcgacttgcc gaattcggag tgaccgatct tcaggcggag agcgccgagg ctgtcccaga
     7561 cgatcttggc cttgtcggcg ccgaagaaca cgacgtcgcc gtcctgggcg ccggtgcgtt
     7621 cgatgatcgt gttgagctct tcatcggaga ggttcttgac gatcgggctc tggagacctt
     7681 cgcggccctt ggagagatcg ttgatcttga tgtaggcgag acccttggca ccgtagatct
     7741 tgacgaactc ggtgtaggcg tcgatttcgg aacgcggcat ggaggcggcg cccggaacgc
     7801 gcagggcgac gaccttgccg ttgtgcaggg acttgacgcc ggagaacacc ttgaacgtgg
     7861 agttctcgac gacgtcggtc acttcgacga gcttcaggtt gacgcgaagg tcgggcttgt
     7921 cggagccgta ctgggccatg gcgtcgtccc agttcatgac ggggaacggg ttcttgaggt
     7981 cgacgtcgag gacttccttg aagaccgtgc ggatgagttc ttccatcagg gcgcggattt
     8041 cgtccatgtt gaggaaggac gtttcaatat cgacctgcgt gaattcaggc tgacggtcgg
     8101 cgcgaaggtc ttcgtcgcgg aagcacttga cgatctgata gtagcggtcg aagccggcga
     8161 ccatcaggag ctgcttgaag agctgcggag actgcggcag ggcgaagaag cagccgtcct
     8221 gaacgcggga cggaacgagg tagtcgcgag cgccttcagg cgtggagcgc gtcagcatcg
     8281 gggtttcgat gtcgatgaag ccgttcttgt cgaggaactt gcggaaggcc atggcagcgc
     8341 gatagcgcgt gcggaggttc ttctgcatga caccgcggcg aaggtcgagc acgcggtacg
     8401 tcagacgcgt ggtttcggag aggttttcgt catcgaggtg gaacggaggc gtgaccgacg
     8461 ggttgaggat ctcgagttca tggcagagaa cttcgatgcc gccggagacg agcgtttcgt
     8521 tcttcgtgcc ttccggacgg gcgcgcacga cgccgacgac cctgagacag aattcgttgc
     8581 ggcacttgtc ggccgtggcg aagacttcgg gacggtccgg gtcgcagacg acctgaacga
     8641 tgccctcgcg gtcgcgaagg tcgatgaaga tcacgccgcc gtggtcgcga cgacgatgag
     8701 cccagccgaa aagagtcacg gtctgaccga tgaaggattc atcaaccagg ccagaatata
     8761 cggttcgcat ataagctccg agaaaaagac caacgcgcga tacgagagct gttggccaca
     8821 cgattacaac gacaatgcta tggctttgca gagccgcaat ggtgcatgcg gcggcgcgac
     8881 gggcgcaccg ctgcggaatc agtccgcgcc gcctcaaggg ggcgaaagcg cacaatgatt
     8941 aagtttactg ccaaagaatg tcgaaagacg agaaaacgcg gcagtttggg gtattttatc
     9001 tagaaccgct cacatgaaaa cgcacaaagg ccgttgcctc ggactgacag tcctggacat
     9061 ccggcctttt atcgttttga cgggcggcgt tggcgcactg cttacttcga gctgtcaggc
     9121 ttgcggtgag aggccggaag ggctttgtgc ccgccgttga agtctgtggc ggcccagccc
     9181 gtgcccctga gctcgaagcc gggggcggag agttccttca ccaacgcatc cttgccgcac
     9241 tgagggcact tcgtcagagg cttgtcactc atcttctgaa gaaaatcctt ttcaaagccg
     9301 cagttcgtgc atcgataagc gtagataggc atggtatgaa agtttggaaa gtgaacgtgg
     9361 aggaaacaga ttataaggaa gtgacggcgg cagaggcttg aaaaagcgga aaaaaggccg
     9421 ccgcatcaaa gatacggcgg ccttgtcagt ggccggattc agcgaagagg attattcctc
     9481 gtcgtcctcg cggtggaact tgcctgcaag ttcggcctgc ttctgggccg gaatcggctg
     9541 gtagctgtca agctccatgg accagctgcc cgtgccctgc gtgagggcgt gcaggcgggt
     9601 ggcgtagccg tcgagttcgg aaagcggcac gacgccggtg atttccatca ggccgccggg
     9661 cagggagtcg gtgccggaca cctggccgcg acggctggcg aggtcgcctg caaggtcgcc
     9721 catgtacatg tcgggcgccg tgatctcgat gcggacgatc ggttcgagga tcttcggctg
     9781 ggcgttggcg agcgcgtcaa ggaaagcctt gcggccggcg gcgacgaagg cgacttcctt
     9841 cgagtcgacg gggtggctct tgccgtcgta gacggtgacc ttgacgtcct ggatcgggta
     9901 cccggcgagg taaccggagt cgagcacttc gcggacgccc ttttcaacgg cggggatcaa
     9961 gttgaacgga atcacgccac ccttgacctc gtcgtggaac tcgaagcccg cgccgcgttc
    10021 gagcggctcg atgcgcaggt agacttcgcc gaactggcca gcgccgcccg tctgcttctt
    10081 gtggcgggca tggccttcgg ccggcgcggt aatggtctcg cggtacggaa cgctcggcgt
    10141 gccggtgacg acttcaagct tgaagttgac cttcatgcgg tcgaggatcg aacggacgtg
    10201 catttcggtc aggccgcgga tcacggtctc gttgagcacg gcgtcatggt cgacggccat
    10261 cgtcggatct tcggagagca tcttgttgag gacttcgccc atgcgggctt cgtcgccgcg
    10321 gcgggatggt gcgatcgcga ggccgaacat cggcttcggg aagttgatcg gacgcatgcg
    10381 cacgtcgcag aggttcggat ccgagtgaag gatcgagccg taggtgagtt catcgatctt
    10441 ggcgagggcg ccgatgtcgc ccgggctgag gaagtcggtt tcggtcgtgt tcttgccctg
    10501 gaggagcagc gggtgagcgg ctttcacgcc cttcttgttg tcgtcgacgt agagcgtggt
    10561 gtccttctgg attgtgccct gatggacgcg gaagatgccg atcttgccga tgtacgggtc
    10621 attgacgacc ttgaagacct gggcgacgac gggcagatcc ttttccggac gcgtgatgaa
    10681 cggcgtgccg tcggcatgga tgaagagagc gtcgttggct tcggccgggc tcggaagatg
    10741 gcggatgatg accttcatga agtcgcggat gccgatcagg ttcttcgcgg agacgaagca
    10801 gacggggatg accgtgcctt cacgcagggc cttcgtgatg agcgggtgca gggtgcgagg
    10861 gtcgacgctg ccttcctcga gatagtgctc gagcatttcg tcgtcctgct cgatgatctt
    10921 ttcaatgaag gcgcgatgga cttcaccgac gctgagaatg tcggcttctc cttcgtcctt
    10981 gtcgaagcag tcgatgacga tggagcggtt gttggcggga aggttgatcg ggagaacgcc
    11041 cggaccccag acttcctgca ggtccagaag cagctgttcg aggtcgacgt cgggtgcgtc
    11101 gaacttgttg atcacgatca tgcggcacag accgcgttcc ttggcgatgt ccatcatgcg
    11161 gcgcgtcaga agctcgacgc ccttggtggc gtcgatgacg gcgcagacgg acttgacggc
    11221 gtcaagcgcg gacatatcct gaccgatgta gtcggggtac cccggggtgt cgagcacgtg
    11281 aatgcgcacg ggggtgaggt cgggaagagc cgtgtcgacg tgagcgacgg ccagacggat
    11341 ggagtgctgg acctgctttt caagcgcgtc gttgtcgcac atcgtgttgc cgcgctcaat
    11401 ggagccgagt tcggaaatca tgccggtgcg gtagagcatg gcttcgatga gggaagtctt
    11461 gccgcaggag ccgtgcccca ccaacgctac ggtacgaaga ttctcggtca gatagttgct
    11521 cattgctgtt ctcctcttcc agaacaattt tttgaacggc tggtcaagcc gtcagccttt
    11581 caattgtgcg cccgcgggag tggagatagg gtgttatgac gaaagatgtt gacttatgtc
    11641 gacaggatga gagaagacac tggttttttc gggcgaaaag gggcaaactg tatagttgcg
    11701 ccaaaacaca aaagccttgc cccgatgatt tcgcggcaag gctttttgct ttcagggccg
    11761 ttcagggctc gtcgca
//
LOCUS       DBMDTI010000037        11701 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1650_length_11701_cov_2.799569, whole genome shotgun sequence.
ACCESSION   DBMDTI010000037 DBMDTI010000000
VERSION     DBMDTI010000037.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 11701)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11701)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11701
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1650_length_11701_cov_2.799569"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            84..1133
                     /gene="dinB"
                     /locus_tag="ACZPB6_03325"
     CDS             84..1133
                     /gene="dinB"
                     /locus_tag="ACZPB6_03325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR1183537.1"
                     /translation="MDAFYASVEQRDRPELRGRPIAVGHAQGRGVVATASYEARRFGV
                     HSAMPSSRARELCPELIFVEGRMSHYKAVSCQIHEIFRRYTDVIEPISLDEAFLDVTV
                     NKQGLALGVEVAVAIKRAIREELGLVASAGVSYNKFLAKVASDWRKPDGLCTIHPERA
                     IDFIDRLPVEAFWGVGPATAARLHALGIRTAPELRAVPLSDLMRHFGQSGYDFYRFVR
                     GIDDRPVRSSRVRKSVGCERTFERDVRDPVRLRGIIGELAAELAFRLGRADFAGSTLS
                     LKVRSHDFTTRTRSVAAEVPYDANAMTRLAEDACRLLAGMEIPKGGVRLLGLAVGSPA
                     QRDDGQPSLFEGLEA"
     gene            complement(1208..1987)
                     /locus_tag="ACZPB6_03330"
     CDS             complement(1208..1987)
                     /locus_tag="ACZPB6_03330"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HIR1183538.1"
                     /translation="MAQAFNYADGLRWFGEGVRALRNRPLGLVGIVVFYVLISGLLSG
                     LPVVGTILAGVWMPFGAVLTGFAAREALADRLPGYAPLLAAFRKQGMRLHLIAVGLIS
                     AGVLELVMLVFQLLSADQIAQWKMVEGAIDPESVAAHFPSAGVAAALVLYTPLFMATL
                     FAPLLIADAGQGAGKSFFYSFFGILRNFSACLTTAVLLVLMTSAAVFLTVGAGLAAGL
                     GGATTYITPIIAILVTTIAQACVWPMYRDLFGPKNLFERLD"
     gene            complement(2000..2443)
                     /locus_tag="ACZPB6_03335"
     CDS             complement(2000..2443)
                     /locus_tag="ACZPB6_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183539.1"
                     /translation="MTKTRNCLSALCLIAVSTAACAAPSAPNDAPPPYAGDVKVEKPA
                     PQSGPAARKPSAEEANPLLPSKADMEKAREERAHGHLPPKFDRQTKIEEVRDENNRVT
                     EYVVTPGTTQIPYRIENRAERPIDSSPAGNSKSTLGTTKFIELGW"
     gene            2744..5587
                     /gene="polA"
                     /locus_tag="ACZPB6_03340"
     CDS             2744..5587
                     /gene="polA"
                     /locus_tag="ACZPB6_03340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR1183540.1"
                     /translation="MSKILLIDGSNYLFRAFHGLPDLRTSSGEPTGAVRGFLGMLGKV
                     WALAKPDHAVIIFDAQGKNFRHEMFPEYKSNRPPMPDDLRVQIEPLHGILKDLGWPVV
                     SVPGVEADDVIATIAEKARAAGMKSIIATGDKDMAQLVDDDIVLINTMNVKFYDREGV
                     IEKYGVPPERIVDYLSLMGDKVDNVPGIEKCGPKTAAKWIAEYGDLEGVKAHAGEVKG
                     KAGEYLRAGLAFLDDARRLTTICRTVKLPEGCVPEALVVKPADPDAVSAFCRRWEMSL
                     STLKRAAPSGIGASAEPAATGTAEPDDLGGLFSREAFAAEALAAKPAPKPAPAKKAAP
                     AAPVMQVSFSPALAERPDELPVTEIADRMTLELFAGRLLDESRAMPVALTMLWDGDAR
                     HAEPAALGVALSIKDVFVIRFTEELPVAHVMDVMRPWLEGSAAKVAHDAKTLAHVFLA
                     QGVRLGGMLHDTMLMSYVLEAHLKHELPLLAARSLARSIPTRESVVGKGAKAIAAKDA
                     DPKAVVELLGEEAAAIRALHAVLMTRLDLEDGLMAIYETLEQPLIGVLGRMEEAGVAL
                     DDFRLSQQSEALTVRIDGLVADAYKAAGHEFNLSSPKQLAQVLFTELGLPSSKKTGTG
                     TPSTDEEVLTELALDYPLPKLILEHRRLTKLKSTYLDKLPRMVDPKDGRVHTTFGQAV
                     AVTGRLASSDPNLQNIPVRTDEGRAVREAFVAESGNVIISADYSQIELRIMAHLSQDK
                     GLLDAFARGEDIHRATAAEVFGRKPEEVTADERRMAKVINFGLIYGMSAFGLAQNLGI
                     ERRMAAHYIDEYFARYPGVRVYMDAKRAEAHERGYVETAFGRRLWIPEIASSRKPVQA
                     AAERAAINAPMQGTAADLIKKAMIAVDAWLRAEGLRSRLVLQVHDELILEAPVEEAEL
                     VRRKVPELMAAVASLSVPLVAEVGMGDSWGAAH"
     gene            complement(5679..6104)
                     /locus_tag="ACZPB6_03345"
     CDS             complement(5679..6104)
                     /locus_tag="ACZPB6_03345"
                     /inference="COORDINATES: protein motif:HMM:NF015669.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HIR1183541.1"
                     /translation="MKLRHAAAIVPCAIALTACTAVTSTQNADLAGRSLMWADGATEE
                     CEVPPSIKFGADGKVSGNAGCNLLVGGWTLQGDKLDLSQLGSTRRMCGPELMKMEDAF
                     LSNLGKTVYVETEGDKVRLLDAERKLVMTLVPEAPGVCK"
     gene            6438..6794
                     /locus_tag="ACZPB6_03350"
     CDS             6438..6794
                     /locus_tag="ACZPB6_03350"
                     /inference="COORDINATES: protein motif:HMM:NF033674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HIR1183542.1"
                     /translation="MFKSCLAGLALALALGGAQAEVTARHAYVSFDTVAAAQKNARAD
                     ELVYFRGHITAEPEDGVYVFEDGTGNVRVEIPNEARTGVTLRRDVRYEVFGRALRLPF
                     EELRVQAETLTPISGK"
     gene            complement(6883..8115)
                     /locus_tag="ACZPB6_03355"
     CDS             complement(6883..8115)
                     /locus_tag="ACZPB6_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR1183543.1"
                     /translation="MSSKIKISLAWQMMIGLALGIVVGALVDSSFAQTWLQPLGDLFI
                     RLIRMVVVPLVLATIIAGAAGISDTSKLGRVAVKVLLVYAVTTGIAVACGLLFAGIIQ
                     PGMGLDLSTEGLQAKEVVAPPLVKTLLAIVPINPMQAMAEGSMLQIIFFAVIFGFALS
                     GLGERGKPVLHFFEIVGDVMIRVTHMVMLYAPIGVFGLISFTVSRHGLAVLLPLGALI
                     LTAFLATVVFVGMILLPLVRIATGIPIMTFLKGIFEPWLVAFTTCSSAAALPANLNAA
                     RKLGSTKAISSFSIPLGNTINMNGTAIYMGVCSIFAAEVYGLPLTMADQLTIVLMGVL
                     AAVGTAGVPGAGLIMTTIIFTQVGIPLEAVALIAGVDRILDMIRTSINVVGDVAAAVI
                     VTKMEGDLNSEPYVEGAD"
     gene            8507..9325
                     /locus_tag="ACZPB6_03360"
     CDS             8507..9325
                     /locus_tag="ACZPB6_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014582.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR1183544.1"
                     /translation="MDTLLHILAAGVLTRVLSISVAALLSCRWLAGLSEQLLAVACGL
                     LAAVALTHLIPEAFEMPGVEAHDLGLVMLGTVLLFLFLERIFTHHGHEGCGEAHPGRA
                     VATAVMTGAGLHNFVDGVLIASTFLMDERAGWLVALAVCCHEIPQSTGYMVILKNCGL
                     DARRAVLWCVAAALPTLAGGVAGWAAVSFSREALPFALAASAASFLFITLHALLPEVF
                     RDCRTRRDGLRQLVLFIAGVLLSTVILGIGHDHGHEGHEHEKPGVQLQADPHGH"
     gene            complement(9401..10261)
                     /locus_tag="ACZPB6_03365"
     CDS             complement(9401..10261)
                     /locus_tag="ACZPB6_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1183545.1"
                     /translation="MIKSLWMLVASLLFALMAAFARMGASDFSALELVFYRSAFGAAV
                     IALWVWAQGFTLRTPLVRAHFMRSFLGTFALTIWFYAISKLPLGTAMTLNYTSPLYMA
                     LIVIFLAVKNGESFSRASAAAVVLGFFGVTLVLKPELSPGQTFPALVGLTSGFFAALA
                     YFQIRQLSRMQEPEWRIVFYFTLFGTVWGLGGHLLLAGGMTPVTWDNAPALLGMAVTA
                     TGAQLAMTRSWGSANTLLTAVFQYSAIVFAAGFGLAFFDEPVGLMSAAGIAVILVSGV
                     WAAVIAKKKS"
     gene            10431..10658
                     /gene="xseB"
                     /locus_tag="ACZPB6_03370"
     CDS             10431..10658
                     /gene="xseB"
                     /locus_tag="ACZPB6_03370"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR1183546.1"
                     /translation="MTDLKPVEELTFEEALEELEELTSSMASGEATLKESVAGYARGT
                     ALLKRCRRELEEARRTIETLRAESDDEVAPF"
     gene            10705..11628
                     /locus_tag="ACZPB6_03375"
     CDS             10705..11628
                     /locus_tag="ACZPB6_03375"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR1183547.1"
                     /translation="MTEASENDFKVWMTETARHFEWYADSTLPKPGREPRRLIGAMRY
                     AVLGGGKRVRALLAYAAGAVSGASPEALDRAALALELVHAYSLVHDDMPSMDNDTLRR
                     GKPTVHVQYGEATAMLAGDALQTEAFTVLAAVEPPAAAARLCATLARAAGVRGMCGGQ
                     ALDLAMVGGHPGEAELLRMQAMKTGALIQAAVLCGAQAGDWDRLGDGARGGLAAYADA
                     LGVAFQVVDDILDCTQDTATLGKTAGKDEKDDKPTWVSLLGLEGARTRARELHDRANA
                     ALALVSADPAVPEGAVDRLAQIADYVIARSH"
BASE COUNT         2127 a         3550 c         3963 g         2061 t
ORIGIN      
        1 ttcccgcatg cgtttccgca ggaaacgggg ccgaaggcct cacgcttgtg cgcaacgtct
       61 cgcgcaggat cgtgcacgtc gacatggacg cattctatgc ctcggtcgag cagcgtgacc
      121 gccccgagct tcggggacgc ccgatcgcgg tcggacacgc tcaggggcgc ggcgtcgttg
      181 cgacggcaag ctacgaggcg aggcgcttcg gcgtgcacag cgccatgccg agttcgaggg
      241 cgcgcgagct ctgtcccgaa ctgatcttcg tcgagggacg aatgagccac tacaaggccg
      301 tctcctgtca gatccacgag atcttcaggc gctataccga cgtgatcgag cccatcagcc
      361 ttgacgaggc ctttctcgac gtgaccgtca acaagcaggg gctcgccctc ggcgtcgagg
      421 tggcggtcgc catcaagcgc gcgatccgcg aggagctcgg ccttgtggcg agcgccggcg
      481 tctcctacaa caagttcctc gccaaggtcg cttccgactg gcgaaagccc gacggtctct
      541 gcacgatcca tcccgaaagg gcgatcgact tcatcgaccg cctgcccgta gaggccttct
      601 ggggcgtggg ccccgccacg gccgccagac tccatgcgct cggcatcaga acggcgcctg
      661 aactccgagc cgtgccgctc tccgatctga tgcgccactt cggacagtct ggctacgatt
      721 tctaccgctt cgtgcgcggg atcgacgaca ggcccgtgag gtcgagccgc gttcgcaaga
      781 gcgtcgggtg cgagaggacc tttgagcggg acgtcaggga tcccgtgcgc ctgaggggga
      841 tcatcggaga gcttgccgcc gagcttgcct tcaggctcgg ccgcgcggac tttgcaggaa
      901 gcacgctttc actcaaggtg agaagccacg acttcacgac gcgcacgcgc tcggtggcgg
      961 ccgaggttcc ctatgatgcg aacgccatga cgaggctggc ggaggatgcc tgcaggcttc
     1021 tggcgggcat ggagattccg aagggcggcg tcaggctgct ggggcttgcc gtgggatcgc
     1081 ccgcgcagag ggacgacgga cagccctcgc tttttgaggg gctcgaggcg tgaggatgtg
     1141 aagaaggccg cggtgacgcg gccttccttg atgatgtcga cagggtcgac aatgagctca
     1201 ggcggactca gtcgagacgc tcgaagaggt ttttcggacc gaagaggtcg cggtacatcg
     1261 gccagacgca ggcctgagcg atcgtcgtga cgaggatcgc gatgatcggc gtgatgtagg
     1321 tcgtggcgcc gccgagcccg gcggcaaggc ccgcaccgac ggtgagaaat accgccgcgc
     1381 tcgtcatcag cacgaggagg acggccgtcg tgaggcaggc cgagaagttc ctcaggatgc
     1441 cgaagaagga gtagaagaag ctcttgcccg cgccctggcc cgcgtccgca atgagaagcg
     1501 gcgcgaagag cgtcgccatg aagagtggag tgtagaggac gagcgccgcc gcaacgcctg
     1561 cggacgggaa gtgtgccgcg acgctttcgg ggtcgatcgc cccctcgacc atcttccact
     1621 gcgcgatctg gtcggcggaa agcagctgga agacgagcat gacgagctca agcacgccgg
     1681 cgctgatgag gcccacggcg atgaggtgca ggcgcatgcc ctgcttcctg aaggcggcga
     1741 gaagcggtgc gtatccggga agcctgtctg cgagcgcctc gcgggcggca aaacccgtga
     1801 gcacggctcc gaacggcatc cagacgcctg cgaggatcgt gcccacgacg ggcaggcccg
     1861 agagcaggcc gcttatgagg acgtagaaga cgacgatgcc gacgagcccg agcggccggt
     1921 tcctgagggc gcggacgccc tcgccgaacc agcgcaggcc gtcggcgtaa ttgaatgctt
     1981 gtgccataaa gggatacggt taccagccga gttcgatgaa cttggtcgtg ccgagcgtgc
     2041 tcttggagtt gcccgcaggc gaggagtcga tcggacgttc ggccctgttc tcgatgcgat
     2101 aggggatctg ggtggtgccc ggcgtcacga cgtattcggt gacgcggttg ttttcgtcac
     2161 ggacctcctc gatcttggtc tggcggtcga acttgggcgg cagatggccg tgggcgcgct
     2221 cctcgcgggc cttctccatg tcggccttgc tcggaaggag cgggttggcc tcctcggcgg
     2281 acggcttgcg ggctgcgggt ccggactgcg gagcgggctt ctcgaccttc acgtcgcccg
     2341 cgtacggggg cggcgcgtcg ttcggagccg aaggagccgc gcaggcggcg gtcgaaacgg
     2401 cgatgagaca gagggcggac aggcagtttc tggtttttgt catggctaat cctttgcgcc
     2461 gctcgtcatg ggcttcgcgg aagcggcggc gcggtgaagg gaggcagggc cggcgggcgt
     2521 caggcccatg aatggtcgga atgcgcctgc gagacctcct gcggcgcatc gcggccctgt
     2581 cagtccaatt gtgccacggc gaggcgttca gaggcgacag gcgaagtgtg tccgcatgta
     2641 tggatgcgtc tctggcttcg ccgtggcgga cggctttttc ggctattctt acggcagccg
     2701 gtttttacgg cccattcata catttgcagc aacgacttcc aacatgtcca agattcttct
     2761 catcgacggc tccaactatc ttttccgcgc ctttcacggc cttcccgacc ttcgcacgag
     2821 ttcgggcgag ccgacggggg ccgtccgggg ctttctgggc atgctcggca aggtctgggc
     2881 gcttgccaag cccgatcacg ccgtcatcat cttcgacgcg caggggaaga acttccgcca
     2941 cgagatgttc cccgagtaca agagcaaccg tccgccgatg cccgacgacc tgcgcgtgca
     3001 gatcgaaccg cttcacggca ttctgaagga cctcggctgg cccgtcgttt cggtgcccgg
     3061 cgtcgaggct gacgacgtga tcgcgacgat cgccgaaaag gcgcgcgccg cgggcatgaa
     3121 gagcatcatc gccacgggcg acaaggacat ggcccagctc gtcgacgacg acatcgtgct
     3181 catcaacacg atgaacgtga agttctacga ccgcgagggc gtcattgaaa agtacggcgt
     3241 tccgcctgag cgcatcgtcg actacctctc cctcatgggc gacaaggtcg acaacgtgcc
     3301 cggcatcgag aagtgcggtc cgaagaccgc cgccaagtgg atcgccgaat acggtgacct
     3361 tgagggcgtc aaggctcatg cgggtgaagt gaagggcaag gcgggcgaat atctgcgcgc
     3421 ggggcttgcc ttccttgacg acgcacgccg cctcaccacg atctgtcgta cggtcaagct
     3481 tcccgaggga tgcgtgcctg aggccctcgt cgtgaagccc gccgatcccg atgctgtctc
     3541 cgccttctgc cgtcgctggg agatgtcgct ctcgaccctc aagcgcgccg cgccttccgg
     3601 catcggcgcc tccgccgagc ctgcggcgac agggacggcc gagcccgacg atctcggcgg
     3661 actcttttcc agagaggcct ttgcggccga ggcgcttgcc gccaagcctg ctccgaagcc
     3721 cgctcccgcc aaaaaggcgg cgcccgccgc acccgtgatg caggtgagct tctcgcccgc
     3781 gctggccgag cgtcccgacg agctgcccgt gaccgaaatc gccgaccgca tgacgctcga
     3841 gctgttcgca ggcaggctgc tcgacgagag ccgcgccatg cccgtcgcgc ttacgatgct
     3901 ctgggacggc gacgcccgtc atgcagagcc tgccgcgctc ggcgtcgcgc tttccatcaa
     3961 ggacgtcttc gtgatccgct tcacggagga gctccccgtc gcgcacgtca tggacgtgat
     4021 gcgtccctgg ctcgagggtt ccgccgccaa ggtcgcacat gacgcgaaga cgctcgcgca
     4081 tgtcttcctc gctcagggcg tgaggctcgg cggcatgctg cacgacacga tgctgatgag
     4141 ctatgtgctc gaggctcatc tgaagcacga gctgccgctt cttgccgccc gctcgcttgc
     4201 gcgttcgatt cccacccgcg agagcgtcgt aggcaagggc gcgaaggcca ttgcggcaaa
     4261 ggatgctgat ccgaaggccg tcgtggagct cctcggcgag gaggccgccg caatccgtgc
     4321 gcttcatgcc gtgctgatga cgaggctcga tcttgaggat gggctgatgg cgatctacga
     4381 aacgcttgag cagcccctca tcggcgtgct cggccgcatg gaggaggcgg gggttgcgct
     4441 cgacgacttc aggctctcgc aacagtccga agccctcacc gttcgcatcg acggccttgt
     4501 ggccgacgcc tacaaggcgg cggggcatga gttcaatctg tcgagtccca agcagctcgc
     4561 gcaggtgctc ttcaccgagc tcgggcttcc ctcgagcaag aagacgggta ccggcacgcc
     4621 gtcgacggac gaggaggtgc tgacggagct cgctctcgac tatccgttgc cgaagctcat
     4681 tctcgagcac cgtcgtctca cgaagctcaa gagcacctat ctcgacaagc tcccgcgcat
     4741 ggtcgacccg aaggacggcc gcgtgcacac gaccttcggt caggccgtcg ccgtgacggg
     4801 gcgtctggcg agttcggatc cgaacctgca gaacattccc gtgcgaaccg acgagggccg
     4861 tgcggtgagg gaggcattcg tcgccgagtc gggcaacgtg atcatctccg ccgactactc
     4921 gcagatcgag cttcgcatca tggcccacct ctcgcaggac aaggggcttc tggacgcctt
     4981 cgcgcgcggc gaggacattc accgtgcgac cgcagcggag gtcttcggtc gcaagcccga
     5041 ggaggtgacg gccgacgagc gtcgaatggc caaggtcatc aacttcggcc tcatctacgg
     5101 catgagcgcc ttcggtcttg cccagaacct gggcatcgag cgccgcatgg ccgcccatta
     5161 catcgacgag tatttcgcgc gctatcccgg cgtgcgcgtc tacatggacg ccaagcgcgc
     5221 agaggcgcat gagcgcggct acgtcgagac ggcgttcggc cgccgtctct ggattcccga
     5281 aatcgcgagc tcgcgcaagc ccgttcaggc ggccgccgag cgcgcggcca tcaatgcgcc
     5341 catgcagggc actgcggcgg acctcatcaa gaaggccatg attgcggtcg acgcctggct
     5401 gagagccgaa ggtcttcgct ccaggctcgt cctccaggtg cacgacgaac tgattctcga
     5461 ggcgcctgtc gaagaggccg aactcgtgcg caggaaggtg cccgaactga tggccgccgt
     5521 cgcctcgctc tccgttccgc tcgtcgccga ggtcggcatg ggggacagct ggggggccgc
     5581 gcactgacgg ctttcgatcc tcttccccga gagttgatgc aaaagaaaag ggaggccgtg
     5641 aaaacgggcc tccctttttg gaatggtgaa tccggatctt atttgcagac gccgggggct
     5701 tcgggcacga gcgtcatgac gagcttgcgc tcggcgtcaa ggagcctgac cttgtcgcct
     5761 tcggtctcga cgtagacggt ctttccaagg ttggagagga aggcgtcctc catcttcatg
     5821 agttcgggac cgcacatgcg gcgggtggag ccgagctgag agaggtcgag cttgtcgccc
     5881 tggagtgtcc agccgccgac gagaagattg cagcctgcgt tgccggagac cttgccgtcg
     5941 gcgccgaact tgatggacgg cgggacttca cattcctcgg tggcaccgtc ggcccacatt
     6001 agggagcggc ccgcgaggtc ggcgttctgg gtgctggtga cggcggtgca ggcggtgagt
     6061 gcgatggcgc acgggacgat cgcggcggcg tggcgcagct tcatggcagt tttctccttt
     6121 tttttgatgc ggtcgggact tgaccgcaac aggcaaaaca taccatattg gagatatgaa
     6181 ccgacgtttg catctgtatc agagcgtgtc attcaattcg cgtgtgattc acgggatttc
     6241 acggcatctc ttgccctagg gcaagcatcg gcgcatgctt cacacgccgc aaaggcgcaa
     6301 aatgatggcc catgagaatg tggcgcaggc aaccctcacg gggcttcagg tgaatcctcc
     6361 gcgccggcgc ccgttcgttc atcaatcacg catccacgca ggcgttcctc tgggagcgtg
     6421 cctgcaaagg agaagaaatg ttcaagtcct gtctggcagg ccttgcgctt gccctcgcac
     6481 tcggcggcgc tcaggccgag gtcacggcgc gccacgccta cgtcagcttt gacacggtgg
     6541 ccgccgctca gaagaacgcc cgggccgacg agctcgtcta cttcaggggc cacatcacgg
     6601 ctgagcctga ggacggcgtt tacgtcttcg aagacggcac gggtaacgtg cgcgtcgaga
     6661 ttccgaacga ggcccgcacg ggcgtcactc tgagaaggga cgtccgttac gaggttttcg
     6721 gcagggccct gcgcctgccc tttgaggagt tgcgcgttca ggccgagact ctgacgccga
     6781 tttcgggcaa gtagtccgac gatccgattc cgaaaggaaa acgcccctgc agtcaaggct
     6841 gcaggggcgt tttcctggtt tgtcagagcg gttcagcgaa ccttagtccg ctccctcgac
     6901 atagggttcg gaattgaggt cgccctccat cttggtgacg atgacggcgg cggccacgtc
     6961 gccgaccacg ttgatggacg tgcggatcat gtcgagaata cggtcgacgc cggcgatgag
     7021 cgcaacggct tcgagcggaa tgccgacctg cgtgaagatg atcgtcgtca tgatcagacc
     7081 tgcgcccgga acgccggcgg tgccgacggc ggcgagaacg cccatcagaa cgatggtgag
     7141 ctggtcggcc atcgtgagcg gaagaccgta gacttcagcc gcgaagatcg agcagacgcc
     7201 catgtagatg gccgtaccgt tcatgttgat cgtattgccg agcgggatcg agaaggagga
     7261 gatcgccttc gtggagccga gcttgcgggc ggcgttcagg ttggccggaa gggcggcggc
     7321 ggacgagcag gtcgtgaagg cgacgagcca cggttcgaag atgcccttga ggaaggtcat
     7381 gatcggtatg cctgtcgcga tgcgcacgag cggcagcagg atcataccga cgaacacgac
     7441 ggtcgcgagg aaggccgtga gaatgagggc gccgagcgga agcaggacgg cgaggccgtg
     7501 acggctgacc gtgaacgaga tgaggccgaa gacgccgatc ggggcgtaaa gcatgaccat
     7561 gtgggtgacg cggatcatca cgtcgccgac gatttcgaag aagtgaagca cgggcttgcc
     7621 gcgttcacca aggccggaga gggcgaagcc gaagatcacc gcgaagaaga tgatctgaag
     7681 catggagcct tcggccatgg cctgcatggg gttgatcggg acgatggcga gcagcgtctt
     7741 gacgagcggg ggcgccacga cttccttggc ctgaaggcct tcggtggaga ggtcaaggcc
     7801 catgcccggc tggatgatgc ctgcaaagag caggccgcag gccacggcga tgccggtcgt
     7861 cacggcatag acgagaagca ccttgacggc gacacgaccg agcttggacg tgtcggagat
     7921 gccggcggca ccggcaatga tcgttgccag aacgagagga acgaccacca ttcggatcag
     7981 acgaatgaag aggtcgccga gcggctgaag ccacgtctgg gcgaaggagg aatcaacgag
     8041 cgcgccgact acgataccga gcgcgagccc gatcatcatc tgccaggcca gactgatttt
     8101 tattttggag gacataatct ccacccctag ctgttgttac tcttatatag ccgccgcacg
     8161 gttggaaaga gggtgcaacg acgctgacgg aatgtttgct cccgccttga ctcgattcta
     8221 ttcacctagc tcatatataa gacctagggt aaacgcgtat tcgtcggtgc gtgcttcagt
     8281 ttgcttttgc gggaggttcc gaagggggcg cccaatgtac gtggattgcg gcgtgatgtt
     8341 gatttcgatc aaaaggcagc caatgattat ttacctatcg gcatgggtgc tcttataggg
     8401 agcgtgcatg atggtcgggg cttggcgctc tgtcgtaaaa tagcaacgcc cgaaaacagg
     8461 gtttcaaggc tgccctccgc ctctttgaca gtcaatagaa aacaacatgg atactcttct
     8521 acacatcctt gccgcaggcg ttttgacgag agtcctttcg atttccgttg ccgcgctgct
     8581 ttcctgcaga tggcttgcgg gcctctccga gcagctcctc gcggtcgcct gcgggctgct
     8641 tgccgcagtt gcgctcacgc acctcattcc cgaggccttt gagatgcccg gggtcgaggc
     8701 gcatgatctg ggactcgtca tgctcggcac ggtcctgctc tttctgtttc tggagcggat
     8761 cttcacgcat cacgggcacg agggatgcgg cgaggctcat ccggggcgtg cggtcgcgac
     8821 ggccgtgatg acgggagcgg gactgcacaa cttcgtcgac ggcgtgctga tcgccagcac
     8881 cttcctcatg gacgagcgtg cgggctggct ggtggcgctt gccgtctgct gccacgagat
     8941 tccccagtcg acgggctaca tggtgattct gaagaactgc ggactcgatg ctcgccgcgc
     9001 cgtgctctgg tgcgtggccg cggcgctacc gactctggcg ggcggcgtcg cggggtgggc
     9061 cgccgtgagc ttctcgcgtg aggcgctgcc ctttgcgctt gctgcctccg ccgcctcctt
     9121 tctcttcatc acgcttcatg ccctgctgcc cgaggtcttc agggactgca ggacaaggcg
     9181 agacggcctc aggcagcttg ttctcttcat tgcgggcgtg ctgctgagca ccgtcatcct
     9241 cggcattggg catgaccacg gccacgaggg acatgagcat gagaagcccg gcgtccagct
     9301 gcaggcggat cctcacgggc actgatcctg aaagacctga atgcaagaag gggccgtatg
     9361 gcggggtcat gagtcctagc gtgcgcccct ttcgttttca tcaggacttc tttttggcga
     9421 tgacggcggc ccagacgccc gaaaccagaa tgacggcgat gcctgcggcg ctcatgagac
     9481 cgacgggttc gtcgaagaag gcgagtccga agcctgcggc gaagacgatg gccgagtact
     9541 ggaagacggc ggtgagaagc gtgttggcgc tgccccagga gcgggtcatc gcaagctggg
     9601 cgcccgtagc ggtgaccgcc atgccgagga gagcgggcgc gttgtcccag gtgacgggcg
     9661 tcatgccgcc cgcaagcagc aggtgtccgc cgagtcccca gaccgtgccg aagagcgtga
     9721 agtagaagac gattcgccat tctggctcct gcatgcggct gagctggcgg atctggaagt
     9781 aggcgagggc cgcgaagaag ccggacgtga gaccgacgag cgcggggaag gtctggccag
     9841 gagaaagttc gggcttgagc acgagcgtga cgccgaagaa acccagcacg acggctgcgg
     9901 cgcttgcgcg gctgaaggac tcgccgttct ttacggcgag gaagatgacg atgagggcca
     9961 tgtagagcgg gctcgtgtag ttgagcgtca tggcggtgcc gagcggcagt ttcgagattg
    10021 cgtagaacca gatcgtgagt gcgaacgtgc cgaggaagct gcgcatgaaa tgcgcacgca
    10081 cgagcggcgt cctcagcgtg aagccctgcg cccataccca gagtgcgatg acggcggcgc
    10141 cgaaggccga gcggtagaag acgagttcaa gcgcggagaa gtcggaggcg cccatgcgtg
    10201 cgaaggcggc catcagggcg aagagtagcg atgcgacgag catccagagg cttttgatca
    10261 tgatcggcgg acgattgcgg cgtttggacg gcttgcgtgc gggggcgcag gcggagtccg
    10321 tcagagaaac gctcgggctt taggtacaat gcaaggatat gcccggacaa tgcgggcggc
    10381 gcgctttcgg ccttttcggg ccggacggca tttttggaag aatgacttcg atgacagatc
    10441 ttaaacccgt tgaggaactc acctttgaag aggcgcttga agagctcgag gagctcacaa
    10501 gcagcatggc ctccggcgaa gcgacgctca aggaaagcgt ggcggggtat gcccgcggca
    10561 cggcgcttct gaagcgctgc aggcgagagc tcgaggaggc gcgcaggaca attgagacgc
    10621 tgcgcgccga atccgacgac gaggtggcgc cgttctgatc ccctgtggtt gcggcgcacg
    10681 acaaaagaag gagaggtgaa ctcaatgacg gaagcttccg agaacgactt caaggtctgg
    10741 atgaccgaga cggccagaca tttcgagtgg tatgcggaca gcacgctgcc caagccgggc
    10801 cgcgagccca gacggctgat cggtgccatg cgctatgcgg tccttggcgg cggcaagcgc
    10861 gtgcgcgcgc ttctggccta cgccgcaggc gccgtttcgg gcgcgagccc cgaggcgctt
    10921 gaccgcgcgg cgcttgcgct tgagctcgtg cacgcctatt cgctcgtgca cgacgacatg
    10981 ccctcgatgg acaacgacac gcttcgccgc ggcaaaccca cggtgcacgt gcagtacggc
    11041 gaagcgaccg cgatgctcgc gggcgacgcg cttcagacgg aagccttcac ggtgctcgcg
    11101 gccgtcgaac cgcccgctgc ggccgcacgc ctgtgcgcca cgctcgcccg tgcggccggc
    11161 gtgcgcggca tgtgcggcgg tcaggccctt gatctcgcga tggtgggagg gcatcccggc
    11221 gaggccgaac tcctgcgcat gcaggcgatg aagaccggcg ccctcattca ggccgccgtc
    11281 ctctgcggtg ctcaggcggg cgattgggac aggctcggcg acggtgccag aggaggcctt
    11341 gcggcctatg cagacgcgct cggcgtggcc tttcaggtgg tcgacgacat tctcgactgc
    11401 acgcaggaca cggccaccct cggcaagacc gccggcaagg acgagaagga cgacaagccc
    11461 acctgggtgt cgctcctcgg gctcgagggt gcacgtacgc gcgcccgaga gctgcatgac
    11521 cgcgccaatg ccgcgcttgc gctcgtctcg gccgatcccg ccgtccccga aggcgcggtc
    11581 gaccgtcttg cacagatcgc cgactacgtg atcgcgcgct cgcactaaaa tgacccaact
    11641 tcacatatag gccgccctca agtgactcag caaacgcttc tcgactccat cagatcgcct
    11701 g
//
LOCUS       DBMDTI010000038        11683 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1652_length_11683_cov_2.670178, whole genome shotgun sequence.
ACCESSION   DBMDTI010000038 DBMDTI010000000
VERSION     DBMDTI010000038.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 11683)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11683)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11683
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1652_length_11683_cov_2.670178"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="ACZPB6_03380"
     CDS             <1..182
                     /locus_tag="ACZPB6_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183548.1"
                     /translation="AKTPHERAGVTLRIFESRIRRATPAELLADSPACGLRKAWRGAL
                     EAFMMEEFLQVGGAG"
     gene            complement(268..1380)
                     /locus_tag="ACZPB6_03385"
     CDS             complement(268..1380)
                     /locus_tag="ACZPB6_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012488.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1183549.1"
                     /translation="MKRQVNACRQLTPGPVSIEASVLEAMTCCPSPADAAFISMMEDV
                     RSRLLRVAGMRPAEYAAVLMAGPCAFALEAALTTLIPEESRVLVVCNGARADAACRML
                     ARADIAFEKLETGFEQAAGAAEIAMLLDERPDITHVAVVHVDAVSGLRNDLGEIGRAV
                     RAAGRTFIVDAGVSFGATSIDAEALGIDVLLSVADCGLKGVSGLSFVLARRNILESSE
                     GRARSVSLDLFEGLRRQEAGIENSLPPQAVSALHAALDALDKEGGSAVRETSCRAAAE
                     KLAAGMIRLGFEPCLEEDARSPHVLSFLHPSDTFSFEHLQCVLRAAGWLIASAAIENR
                     SVVSLSTLSGIDDTEADRLLAVFHEYLESLCDCSTK"
     gene            1772..1921
                     /locus_tag="ACZPB6_03390"
     CDS             1772..1921
                     /locus_tag="ACZPB6_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183550.1"
                     /translation="MRANNDRKKNGWAQIFLFIIFLILLALVLNGMGWCIGGVCEVGT
                     DMLLK"
     gene            complement(2099..3052)
                     /gene="bioB"
                     /locus_tag="ACZPB6_03395"
     CDS             complement(2099..3052)
                     /gene="bioB"
                     /locus_tag="ACZPB6_03395"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HIR1183551.1"
                     /translation="MLSLIVERALRGEASPRADLDFLEDSPALDELVEAAHLVTTKCA
                     SRVFNFCTIVNAKSGRCAEDCRWCSQSSKWTTNAPVFPIVPATRGESAAARALEHGIR
                     RLSFVTSGRKLSARELREIRPVVEAAASTGLEVCASMGLLTEPELTGLREAGLVRYHC
                     NLETGPDFFPKVCTTHTQTDKIRTLEAARRAGLELCSGGLFGMGETMADRIDLACGLR
                     RLEIPSIPLNFLIPIPGTPLADRPAMTHDEVVRTVAVFRLVNPSACLRFAGGRQNLAP
                     ETARAALRAGINGAITGDLLTTPGSSVEEERRLVLEAGYSL"
     gene            complement(3205..4311)
                     /locus_tag="ACZPB6_03400"
     CDS             complement(3205..4311)
                     /locus_tag="ACZPB6_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR1183552.1"
                     /translation="MQPNYSVSLPHYSVGPEAYSLVGDIIRPYGTRVAVVGGETAMTK
                     ANPSLLPALEAAGLTVTDVHVYGHDSTVANVEAIKARSGVRNADVILAVGGGRCCDAV
                     KTAADMMGKPVMTCPTVASNCAPVSAIAVLYKEDGSLHGYHFGKACPAHCFINTSVIM
                     DSPEPLFWAGLGDALSKQIEVLYCTRGKHLFHTPLLGAQLACACQEPLLQFGRQALDD
                     FRAKTMSDAFTECVLDIIVSTGVVSNLVTHTEYYYNSSLAHCFYNASMVLPSAHAHLH
                     GEVVSFGNLVLLAYDGQDELLERFLDFNTSIGLPVTLAQVGITTDEQLMKLVSAAEHI
                     KEWSTGPEPAVREQFVAAIRKVDTLGAARLAAAA"
     gene            complement(4615..4947)
                     /locus_tag="ACZPB6_03405"
     CDS             complement(4615..4947)
                     /locus_tag="ACZPB6_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1183553.1"
                     /translation="MMKKLIVVAAAALVAAGAQAASYKDGSYTGEGKGRESQIQVQVD
                     VAGGKIAAVKVLKHGETDMIIAAPIETMIPEIVAKNGVEGVESVGGATMSSDGIKAAV
                     QDALAKAK"
     gene            5236..5799
                     /locus_tag="ACZPB6_03410"
     CDS             5236..5799
                     /locus_tag="ACZPB6_03410"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HIR1183554.1"
                     /translation="MRERKNEKTTGADEEGAVVLTGLAPVVGEAPLILILGSMPGAAS
                     LAAAQYYAHPANRFWPLMALLFPEETARLSSPDYEERLEGLRRSGVALWDTIGSCERA
                     GSLDSSIRNMTPNDVAGLLKSHPTIGTVVLNGSKSAVVWRRHAQREALLERPDLRVLA
                     LPSTSPANARLRLADLEMTWREALAVG"
     gene            5940..7190
                     /locus_tag="ACZPB6_03415"
     CDS             5940..7190
                     /locus_tag="ACZPB6_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1183555.1"
                     /translation="MKPTLSEAMKARLADLCGDDRVKTALQVCVDEADRAMEEQIHIS
                     EIESPTFAEGVRGEEIARLLKEYGLTDVVIDPSGNVVGRRPGTGSGPVLAIAAHLDTV
                     FPAGTNLKVRKEGHLYYGPGIGDNASGLRSMLQVLRALEKAGIETDGDILFVGTVGEE
                     GNGDIRGAKALFDGSRKIDGFMALDMADVFTVQNGATGAHRWRLAIEGEGGHSYLDYG
                     HVPSAIHAMCRAGAMIADLDLPEDPKTTYTIGTIKGGTTVNTIAARCEVDVDMRSVDL
                     PTLEALEAKVLAAFEEAVRIENAHWPKADEAHQLKLVKTQIGNRPAGMRPADCPAVQA
                     ALGALDALGIEARHVKCSSTDANQPMSLNIPAICVGTGGETFLEHSLKEYFDSTDMHL
                     GPQLALLAALAMVGRKGEKGSLAA"
     gene            8285..10315
                     /locus_tag="ACZPB6_03420"
     CDS             8285..10315
                     /locus_tag="ACZPB6_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR1183556.1"
                     /translation="MQSTTRKAAASVLFCSVAMALAAQAVAADAPGVGNKNVNALTQP
                     IYANPDGDEATKGVKTLQDYIVQEKELFDFLFENHPVFKYAAEGRIKGVYKVSTRGSE
                     FLGEGNAQKYTKAAGAKPSASQYRLAAKSILDYPNKFVGPERCGECHAVQYQKWKRSR
                     HAQTVRFPGEHPEVDNDLKKKLYGSDASILPDGITPDVIYATIGTPRTKYGYVDGWLV
                     RGSYHIRDGLLRDGTGTLVAGGNQFSRGWASWLSPERCAEIAKVIPDFPTKMEDFGAS
                     GSHQWGMTSYGSKYEKEFLFQPASSYCEVCHAFKFDFKDKKEFFAALGNPKELQKHTI
                     SKGIACEECHGAGGHLVGAESNGFQTNCERCHQRSNFIPEDVNTEAGQGKIENGFNVK
                     TKSSCPSCGTEGSQLMMSKHYEKGMRCVTCHDPHEVTSNDWKDYYTKPAIKQTCQDCH
                     KEQADVVAQTDTHKKMDCIDCHMPFTMSCENFTAIQRPDMAGFDAVRRSHVFNIKVDP
                     TAKMMNPAEGQSRASNSKGWRIAKDEEGHGYLDLMWSCARTANAEVAVTENKGCHSVF
                     MSELEKGLQYSDQKQAYDDVMEWQTPVKDGYKAAVGAQERIVKLLEVTKLDATAKTEV
                     LMLLDKSKDITKEIEKDGSWGVHAPKYLKQRAETAQAYLDKAQSIIDQAAAK"
     gene            10421..10819
                     /locus_tag="ACZPB6_03425"
     CDS             10421..10819
                     /locus_tag="ACZPB6_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR1183557.1"
                     /translation="MGLIMAISASAAMAGADGPIEPAPVYDEVLNQISLREAEMLFEE
                     GVQFYDANELEIWAQGYIPGAIFFNVKDWKKLLPEDKSTTLVFYCVNPLCTSSEMAAR
                     EVMKLGYTDVRQMPEGIVGWRRSNRPIELP"
     gene            10880..>11683
                     /locus_tag="ACZPB6_03430"
     CDS             10880..>11683
                     /locus_tag="ACZPB6_03430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR1183558.1"
                     /translation="MFTLKKVILSASLALTVSSVAFAQAPQTQIQKESYSMGATLGNV
                     IAGQVYRQTELGAEVDMAAVVQGFNDALKGKTELSDDDMLKILNVRAEQLNKLEEAHL
                     EKVAKANSEKSKAYLAENAKKKGVVTTKSGLQYEVLTEGKGDKPRVEDVVTVNYVGSF
                     IDGKEFENTWQTKEPARFVMMSVIPGLAEGVALMTPGSRYRFTVPAELAYGHDGAGQI
                     PPESALVFDLQLVKVEKVGAHQQSFGTLRKDMFPDNRFVRRNMNTGESFH"
BASE COUNT         2402 a         3354 c         3745 g         2182 t
ORIGIN      
        1 cggcgaagac gccgcacgaa cgtgcgggcg tcacgcttag gatcttcgag agccgcattc
       61 gtcgcgcaac gcccgccgag cttctggctg attcgcccgc ctgtggattg agaaaggcct
      121 ggcgcggagc gctcgaagcc ttcatgatgg aggaattcct ccaagtaggc ggcgctggat
      181 gaatcgattg agatgcgaaa ggggcggttc ccggcggacc cgccccttgt cacgtccttt
      241 tcaagagatg aaggcatgac gcacctatta tttcgtgctg cagtcgcaga gcgactcaag
      301 gtattcgtgg aaaacggcga gcaggcggtc ggcttccgtg tcgtcgatgc ccgagagcgt
      361 cgagaggctg acgacggacc ggttttcaat ggcggcggac gcaatgagcc agcctgcggc
      421 cctgagcacg cactgcagat gctcgaacga gaacgtgtcg gaaggatgaa ggaaggagag
      481 tacgtgcggg ctgcgcgcat cctcctcgag acagggctcg aagccgagtc tgatcatgcc
      541 ggcggcgagc ttttcggcag ccgcgcgaca ggacgtctcg cgcacggcgg agccgccctc
      601 cttgtcgaga gcgtccagcg cggcatgcag ggcggagacg gcctgaggcg gcagggagtt
      661 ttcgatgccg gcttcctgcc tgcgcaggcc ttcgaagagg tcgagcgaga cgctgcgcgc
      721 ccgtccttcg gacgactcga gaatgttgcg gcgggccagc acgaaggaga ggccggagac
      781 gcctttgagg ccgcagtccg cgaccgagag gagcacgtcg atgccgagcg cttccgcgtc
      841 gatgcttgtg gcgccgaagg agacgcctgc gtcgacgatg aacgtcctgc ccgccgcccg
      901 gacggcacgg ccgatttccc cgaggtcgtt cctgagtccc gagacggcgt cgacgtggac
      961 gacggcgacg tgcgtgatgt cgggacgttc gtcgagaagc atggcgatct cggccgcgcc
     1021 cgcagcctgt tcgaatcccg tctcgagctt ctcgaacgca atgtccgcac gcgcgagcat
     1081 gcggcaggcg gcgtcagcgc gggcgccgtt gcagacgacg agcacacggc tctcctcagg
     1141 gatgagcgtc gtgagcgcag cttcgagcgc gaaggcgcag ggacccgcca tcaggacagc
     1201 ggcgtattcg gcgggacgca tgcccgcgac acgcaggaga cgactgcgca cgtcctccat
     1261 catcgagatg aatgcggcat cagcgggaga cggacagcag gtcatggcct cgaggacgga
     1321 tgcttcaatg gagacggggc cgggcgtgag ctgccggcag gcattgactt gtcttttcat
     1381 gatcttcaac ggtcgtttgt tcagtcaagg atgagaggca ttatcgagag gcttcatgac
     1441 gaaacgacgg cggacgaatg acgaatgcat gacggtcggc gcgcctatga ctgttctccg
     1501 gatttcgtgc tgcggggcgt ctgatacgga ttcgcaggga gattttgtga acgcttgttc
     1561 agtggaacgg gcggaaggtc cgcagtgtgg ggacgaggcg gaaatgctga acgcttgttg
     1621 atttgttttc tatttcaaaa tgataataat tatcgggttt tcttgcggac gcctgtgccg
     1681 caatgggtag gattctcaat attcgaatgc ttgacagagc gataacatga aaacgaaccc
     1741 aagggaaaga ctgtgaaaac ggaggttctc aatgcgtgcg aacaatgaca ggaagaagaa
     1801 cggctgggcc cagatattcc tcttcatcat cttcctcatt ctgcttgccc tcgtgctcaa
     1861 cggaatgggt tggtgcatcg gcggcgtgtg cgaagtcggc accgacatgc ttcttaaata
     1921 aagaagagca gatggggcgg aagccccgtg gcccgcgtga atgtggcggg cggcaaccga
     1981 aaggcgtcag gagagaggcg ccttttgtgt tttttgtggg atggattcgg gaaagggcgc
     2041 ggcgaaggcc gcgccctttc cttatggaga gcttcttttg ggaaccgggt ttcggaagct
     2101 agaggctgta gcctgcctcg aggacgaggc ggcgctcctc ctcgacggag ctgcccggtg
     2161 ttgtgagcag gtcgccagtg atggcgccgt tgatgcctgc gcgcagtgcg gcgcgggcgg
     2221 tttcgggcgc gaggttctgc ctgccgcccg cgaacctgag gcatgcggac ggattgacga
     2281 gacggaagac ggcgacggtt cggacgactt catcgtgcgt catggcagga cggtccgcaa
     2341 gcggcgtgcc gggaatcggg atgaggaagt tgagcggaat cgaggggatc tcgaggcggc
     2401 gcagcccgca ggcgaggtcg atgcggtccg ccatggtctc gcccatgccg aagagtccgc
     2461 ccgagcagag ttcgagtccg gcgcggcggg cggcctcaag cgtgcggatc ttgtcggtct
     2521 gggtgtgcgt ggtgcagacc ttggggaaaa agtcggggcc cgtctcgagg ttgcagtggt
     2581 agcgcacgag tcccgcctcc cgcaggcctg tgagttcggg ttcggtgaga aggcccattg
     2641 aggcgcagac ttcaagtccc gtggaggcgg cggcttcgac gacggggcgg atttcacgca
     2701 gctcccgtgc ggagagcttt ctgcccgagg tcacgaagga gagcctgcgg atgccgtgtt
     2761 cgagggcccg cgcggcggcg gattcgcctc gtgttgcggg cacgatggga aagacggggg
     2821 cattcgtggt ccattttgag gattgcgaac accagcggca gtcttcggcg catctgcccg
     2881 acttggcgtt gacgatcgtg cagaagttga agacgcggga ggcgcacttc gtcgtcacga
     2941 ggtgcgcggc ctcgacgagt tcgtcgaggg cggggctgtc ttcaagaaag tcgaggtcgg
     3001 cgcgcggaga ggcttctcct cgaagggcgc gctcgacgat gaggctgagc atggcggcat
     3061 tccggtcggg ttggacgagc gacaactgta gcgcaggacg tccttcggga gagcggcggc
     3121 tttgcaagcg tttgttgacg gcgcgaaaaa agggacggcg tccgggcagg acgccgtccc
     3181 tttcaggtct gaatttcagg gcaattaggc cgcggcggca aggcgtgcgg cgccgagggt
     3241 gtcgaccttg cggatggcgg cgacgaactg ttcgcgcacg gcgggttcgg gacccgtgga
     3301 ccattccttg atgtgttcgg cggcgctgac aagcttcatg agctgctcgt cggtggtgat
     3361 gccgacctgg gcgagcgtga cgggcaggcc gatgctggtg ttgaagtcga ggaagcgttc
     3421 gaggagttcg tcctggccgt cataggcgag gagcaccagg ttgccgaagg agacgacttc
     3481 gccgtgcagg tgcgcgtggg cggacggaag gaccatcgag gcgttgtaga agcagtgggc
     3541 gagggacgag ttgtagtagt actcggtgtg cgtcacgagg ttggagacga cgcccgtcga
     3601 gacgatgatg tcgagcacgc attcggtgaa ggcgtcggac atggtcttgg cgcggaagtc
     3661 gtcgagggcc tgacggccga actgcaggag cggctcttgg caggcgcatg cgagctgggc
     3721 gccgaggagc ggtgtgtgga agaggtgctt gccgcgcgtg cagtagagca cttcgatctg
     3781 cttggagagc gcgtcgccga ggcccgccca gaagaggggc tcggggctgt ccatgatgac
     3841 ggacgtgttg atgaagcagt gggcggggca ggccttgccg aagtggtagc cgtgcagaga
     3901 gccgtcctcc ttgtagagca cggcgatggc ggagaccggc gcgcagttgg aggcgacggt
     3961 cgggcaggtc atgacgggct tgcccatcat gtcggccgca gtcttgacgg cgtcgcagca
     4021 gcggccgccg cccacggcga ggatcacgtc ggcattcctg acgccggagc gggccttgat
     4081 cgcctcgacg ttggcgaccg tggagtcatg accgtagacg tggacgtccg tgacggtgag
     4141 gccggccgcc tcgagcgcgg gcagaaggga gggattggcc ttcgtcatgg ccgtttcgcc
     4201 gccgacgacc gcaacgcggg tgccgtaggg tcgaatgatg tcgccaacaa ggctgtaggc
     4261 ttcgggaccg acggaatagt gcgggaggct gacggagtag ttcggctgca tgatgaaatc
     4321 cttttctttt ttctgcaggg gttgtgcggg ttcgatgccg tctttttcaa gcggcgtaag
     4381 gtccattgtc acgcggaatc gggccggaga gatccgacaa acgccttagt ggtcctgcgg
     4441 atagtcaggg aggcgtcaaa aaagatgcgc gaagggcacg gacgtgcgat tcgcgtgtgg
     4501 agaaggcgtt tgcaaaataa aaaaagaggg aggtccgcag tgcgaacctc cctctcgtaa
     4561 ggcttcattg aaggaagagg cttccttcag aacccttgct tcgggcgaag aagattactt
     4621 ggccttggcg agggcgtcct gaacggcggc ctttatgccg tcggaggaca tcgtggcgcc
     4681 gccgacgctt tcaacgcctt caacaccgtt cttggcgacg atttccggaa tcatcgtttc
     4741 gatcggagca gcgatgatca tgtcggtctc gccgtgcttg agaaccttga cagcggcgat
     4801 cttgccgccg gcgacgtcga cctggacctg gatctgggat tcgcggccct tgccttcgcc
     4861 cgtgtaggaa ccgtccttgt aggaggcggc ctgggcgccg gcggcgacga gagcggcagc
     4921 ggcgacgacg ataagcttct tcatcatgat gtaaaaacct cggtttgtag tggttttgaa
     4981 atgcgtgtgt gcgtcttccc gaggccggcc gatcggtttg tggagcgggc gtcggagaag
     5041 caggcgcggc gataatagca catcgaaagg caagaaaagc gagcatgccg cagaaaaaat
     5101 tgcaagcact cctttcggac tagagaggtt gtcctaggga agctgtccaa agtcaaatcc
     5161 ggcacggggc gacatttata tataaggatc gatgcctgtg tctgagaaca tttttgtcgg
     5221 gggggggaaa ggtccatgag agaaaggaaa aacgaaaaga cgacgggagc ggacgaggag
     5281 ggcgccgtcg tgctcacggg actcgcgccc gtcgtgggcg aggcgcccct catcctgatt
     5341 ctcggtagca tgccgggagc ggcgtcgctc gccgcagcac agtactacgc gcatcccgcc
     5401 aatcgcttct ggccgctgat ggcgctgctg tttccggagg agacggctcg tctctcgtct
     5461 cccgactatg aggagaggct cgaaggactc aggcggtcgg gcgttgcgct ctgggacacg
     5521 atcggctcgt gcgagcgggc gggaagcctc gacagcagca tccggaacat gacgcccaac
     5581 gacgtggcgg gtcttctgaa gtcgcatccg acgatcggga cggtggttct caacggctca
     5641 aagagtgcgg tggtctggag gcggcatgcg cagcgggagg cgctcttgga gcggcccgac
     5701 cttcgcgttc tcgcgcttcc ctcgacgagt cccgcgaacg cgcgcctgcg gctcgctgat
     5761 cttgagatga cctggcggga ggcgctggct gtcgggtaaa acccgcttgg ccatagggtt
     5821 tgggaggatg gcgaaatttc ggtacggatg ggtaaaattt ccggattgcc gccctgcgga
     5881 tatccaccaa cgattgtccc gcagggcgca acccgacttg aaaccataag gagacagaca
     5941 tgaaaccgac gctttccgaa gcaatgaagg cccgccttgc cgacctgtgc ggcgacgacc
     6001 gcgtgaagac cgccctgcag gtctgtgtgg atgaagcgga ccgtgcgatg gaggagcaga
     6061 ttcacatttc cgagattgaa tcccccacgt ttgccgaagg cgtccgcggc gaggaaatcg
     6121 cccgtcttct gaaggaatac ggtctgaccg acgtcgtgat cgatcctagc ggcaacgtcg
     6181 tcggccgtcg tccgggcacg ggctcgggcc ccgtgctcgc catcgccgcc caccttgaca
     6241 cggtcttccc ggccggtacg aacctcaagg tccgcaagga aggtcatctc tactacggtc
     6301 ccggcatcgg cgacaacgcc tccggcctcc gttccatgct tcaggtgctc cgcgcccttg
     6361 aaaaggccgg catcgagacg gacggtgaca tcctcttcgt cggcacggtc ggcgaggaag
     6421 gcaacggcga catccgcggc gccaaggccc tcttcgacgg ctcccgtaag atcgacggct
     6481 tcatggccct cgacatggcc gacgtcttca cggttcagaa cggcgccacg ggcgctcatc
     6541 gctggcgtct tgccatcgag ggcgaaggcg gccactccta cctcgactac ggccacgtgc
     6601 cctccgccat ccacgcgatg tgccgcgccg gcgcgatgat cgccgacctt gacctgcctg
     6661 aggatcctaa gaccacctac acgatcggca cgatcaaggg cggcacgacc gtcaatacga
     6721 tcgccgcgcg ctgcgaagtc gacgtcgaca tgcgcagcgt cgacctgccg accctcgaag
     6781 cgctcgaagc caaggtgctc gccgccttcg aagaggccgt tcgcattgaa aacgcccact
     6841 ggcccaaggc cgacgaagcc caccagctca agctcgtcaa gacccagatc ggcaatcgtc
     6901 ccgccggcat gcgtcccgcc gactgcccgg cggtccaggc cgccctcggc gcccttgacg
     6961 ccctcggcat cgaagcccgc cacgtcaagt gcagctccac cgacgccaac cagccgatgt
     7021 ccctcaacat cccggccatc tgcgttggca cgggcggcga gaccttcctc gaacacagcc
     7081 tcaaggaata cttcgactcg accgacatgc acctcggccc gcagctcgct ctgctcgctg
     7141 cgctcgccat ggtcggccgc aagggcgaaa agggctccct cgccgcctga ggcaggcccc
     7201 tgcagcatga agagggtctg agcctcaggc ctgtcttctg aatgaaaagg acggacacct
     7261 ccgcggtctc cgtccttttt ttgtgcccgt gaaaaaccat ttccgcatgt gtccggcggt
     7321 tctgcaaaac tgaacgaaac cgggatgccc gccttcgtcg gaccggatcg agggccatgc
     7381 tgatgaatgc atgccgcctt catgcatcac catgaacaac tgttcaggag ggcttcaggg
     7441 agcgttttac aaccttaaga ttcgcgcccc ggcaaagcgg ctcgagcctt cgcccgagtg
     7501 cgggaaaatt cgtgccgaat cgaaaaaacg gcactctttc aggcgaagag aaggtgcgcg
     7561 ggcgctgttt caatgaatcc gaacacgacg ttcggacctt aagataagcc gttttgtacg
     7621 attggaaaaa taaatcgaga gatcttagtc atagaggatc gcaatcgaaa cggcggtttt
     7681 tgcaggtcga catatccccc tttcgctatg ttcggaaaga tcgaacgatg ggtttaagca
     7741 aatgtaaatg gctaagtgaa aactaggggc tagcgagtag agacgccgtt ttgcgaaacc
     7801 atgcgttccg ataagtgaaa cggaacctcg ccaaacctta gggatttggc gtcgaatact
     7861 cctaaagagg tagttcctca gagaggggtg aaaagggacg ggaaaaggat ttcccagtac
     7921 cattgatttt tcaaaatgcc tgtgggacag ttgtttttgt ggtgttatgg tggccgtgtc
     7981 agggtttgct tgcccctgat tgatctaggt caactgagat gcgaaacagg gcattttcag
     8041 aattacctaa gcaaatcgac tgatgcatca taccgttgcg gttcttcggg accgcattga
     8101 ccccattgcc tctcctcgtt tcccggggaa gggataacaa tccccgcccg attctcagat
     8161 cggaaaggga agacaaccaa aaatcgatgt tgctcgtcgc gctgaagacg acggcctctt
     8221 cttcacgcga tgatgccttt tccgatctgg ggaacggaga agacagacat taggagataa
     8281 aactatgcaa tccactacca gaaaagcagc ggcttccgtc ctgttttgct cggtcgccat
     8341 ggctctggcc gctcaggctg ttgctgcgga cgccccgggt gtcggtaaca agaatgtgaa
     8401 cgcgctcacg cagccgattt acgcgaaccc ggacggcgac gaagccacga agggcgtcaa
     8461 gacgctgcag gactacatcg tccaggaaaa ggaactcttc gacttcctct tcgaaaacca
     8521 ccccgttttc aagtacgcag ccgaaggccg catcaagggc gtctacaagg tgtccacgcg
     8581 cggttccgaa ttcctcggtg aaggcaatgc ccagaagtac acgaaggccg ccggcgccaa
     8641 gccgtccgct tcccagtacc gtctcgctgc caagtccatt ctcgactacc cgaacaagtt
     8701 cgtgggtccg gaacgttgcg gcgaatgcca cgcggtccag taccagaagt ggaagcgctc
     8761 ccgtcatgct cagacggtcc gcttcccggg cgaacatccg gaagtcgaca acgacctgaa
     8821 gaagaagctg tacggctccg acgcttcgat ccttcccgac ggcatcacgc cggatgtcat
     8881 ctacgccacg atcggtacgc cgcgcacgaa gtacggctac gtcgacggct ggctcgtccg
     8941 cggttcctac cacatccgtg acggtctcct ccgcgacggt acgggtacgc tcgtcgccgg
     9001 cggcaaccag ttctcccgcg gctgggcttc ctggctctcc ccggaacgct gcgctgaaat
     9061 cgccaaggtg atccccgact tcccgaccaa gatggaagac ttcggtgcgt ccggctccca
     9121 ccagtggggc atgacctcct acggttccaa gtacgaaaag gaattcctct tccagccggc
     9181 ttcctcctac tgcgaagtct gccacgcctt caagttcgac tttaaggaca agaaggaatt
     9241 cttcgccgct ctcggcaacc cgaaggaact ccagaagcac accatctcca agggtattgc
     9301 ttgtgaagaa tgccacggcg ccggcggcca ccttgttggt gccgagtcca acggcttcca
     9361 gacgaactgc gaacgctgcc accagcgctc caacttcatc ccggaagacg tgaacacgga
     9421 agccggtcag ggcaagatcg aaaacggctt caacgtcaag acgaagtcct cctgcccgtc
     9481 ctgcggtacg gaaggctccc agctcatgat gtccaagcat tatgaaaagg gcatgcgctg
     9541 cgttacctgc cacgacccgc atgaagtcac cagcaacgac tggaaggact actacaccaa
     9601 gccggctatc aagcagacct gccaggactg ccataaggaa caggctgatg tcgtcgccca
     9661 gaccgacacc cacaagaaga tggactgcat cgactgccat atgccgttca cgatgtcctg
     9721 tgaaaacttc acggccatcc agcgtccgga catggccggc ttcgacgccg tccgccgttc
     9781 gcacgtgttc aacatcaagg tcgacccgac cgccaagatg atgaacccgg ccgaaggtca
     9841 gtcccgcgcc tccaactcca agggctggcg cattgccaag gacgaggaag gccacggcta
     9901 cctcgacctt atgtggtcct gcgcccgtac ggccaacgct gaagttgccg ttacggaaaa
     9961 caagggctgc cactccgtct tcatgtctga acttgaaaag ggtctccagt actccgatca
    10021 gaagcaggcc tatgacgacg tcatggaatg gcagaccccg gtcaaggacg gctacaaggc
    10081 tgccgtcggc gctcaggaac gcatcgtgaa gcttcttgaa gtcacgaagc tcgacgccac
    10141 cgccaagacc gaagtcctga tgctcctcga caagtcgaag gacatcacga aggaaatcga
    10201 gaaggacggc tcctggggtg tccacgctcc gaagtacctg aagcagcgtg ctgaaaccgc
    10261 tcaggcttac cttgacaagg cccagtcgat catcgaccag gctgccgcca agtaagtttg
    10321 gaagtcacgt ttgcttccag ccttaaatcc ccgggcctct catgaggtcc gggggaaaga
    10381 ccaagaagga attatgaaag cgcatctgaa acggtcggtc atgggtttga tcatggccat
    10441 cagcgcatcg gccgccatgg ccggtgccga cggtccgatc gagccggccc ccgtctatga
    10501 cgaagttctc aatcagattt cgcttcggga agctgaaatg cttttcgagg aaggcgtgca
    10561 gttctacgat gccaacgagt tggagatctg ggcccagggt tacatccccg gcgcgatctt
    10621 cttcaacgtg aaggattgga agaagctgct ccccgaggac aagagcacca cgctcgtgtt
    10681 ctattgcgtc aacccgctct gcacgagcag cgagatggca gcgcgcgaag tcatgaagct
    10741 tggttatacg gatgttcgtc agatgcccga gggcatcgtc ggctggcgtc gttcaaaccg
    10801 gccgatcgag cttccgtaag atgcgtgcct cgcctcaggg cgggacggta attcacacag
    10861 acattcaatt gagaaaatta tgtttaccct gaaaaaagtc attctctctg cctcgcttgc
    10921 gctgaccgtg agctccgtgg catttgcaca ggctccgcag acgcagatcc agaaggagtc
    10981 ttacagtatg ggtgctacgc tcggcaacgt cattgccggg caggtctatc gccagaccga
    11041 actgggcgcc gaagtcgaca tggctgcggt cgtccagggc ttcaacgatg ctctcaaggg
    11101 caagaccgag ctttccgacg acgacatgct gaagatcctc aacgtgcgcg ccgaacagct
    11161 caacaagctc gaagaggctc acctggaaaa ggtcgccaag gccaattccg agaagtccaa
    11221 ggcttacctc gctgaaaacg ccaagaagaa gggcgttgtc acgaccaagt ccggcctgca
    11281 gtacgaagtc ctgaccgaag gcaagggcga caagccccgc gtggaggatg tggttaccgt
    11341 caattacgtc ggatccttca tcgacggcaa ggagtttgaa aacacttggc agacgaagga
    11401 accggcccgc tttgtgatga tgtccgtgat tccgggcctc gccgaaggcg tcgccctcat
    11461 gaccccgggc tcccgctacc gcttcacggt tccggccgag cttgcctacg gtcatgacgg
    11521 cgcaggtcag attcctcccg aatccgccct cgtcttcgac ctccagctcg tcaaggtcga
    11581 aaaggttggt gctcatcagc agagcttcgg cacattgcgt aaagacatgt tccccgataa
    11641 tcggtttgtt aggcggaata tgaacaccgg agaaagtttc cac
//
LOCUS       DBMDTI010000039        11613 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1662_length_11613_cov_2.410441, whole genome shotgun sequence.
ACCESSION   DBMDTI010000039 DBMDTI010000000
VERSION     DBMDTI010000039.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11613)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11613
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1662_length_11613_cov_2.410441"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="ACZPB6_03435"
     CDS             <1..210
                     /locus_tag="ACZPB6_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR1183559.1"
                     /translation="KTKLPETFTSWHDPDLVIPATLPADVLPGFFVLDGHGHLVGFVR
                     GFVDWADPAVPAYFEKLADKYAVRK"
     gene            424..4026
                     /locus_tag="ACZPB6_03440"
     CDS             424..4026
                     /locus_tag="ACZPB6_03440"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HIR1183560.1"
                     /translation="MIHTVYSNSYEVLRVCLMNNIEALGVSTGGSGGASLFERAFEKV
                     PVITPNNAVEEDLRRAVADRDGICAGIDFMKLSSWMGFFSKEPMANIVGNEADWMIWD
                     LLRRTGSGSFREGPGRERLRHYLEGRSDEDVWHLARRIAEVFVVYSTYRLDWVLDWLG
                     MHPEMLNDTDARTTEQKVLERHPDFAWQRDLWRELASRPSWRGRRFLEGFPDMLRRLA
                     ASGGSKRVKLENDFTVTLPDALHVFVPFVVPPVMLPIIKAYAASGRDVWFYLLNPTSE
                     YWYDLLPRRLFDWKNAPEGAELDGHPLLADNGGSTRANIDRIWRFTAAPDTAAGLSEL
                     GVSEELDEDTVSRLPQGTRRFLSSDFLKGYAGRHQEIRADMAVANDSYYIERNSTDLL
                     SRVQDSILNLDADLKKTGGGAPLFREDDRSIRFMRSPTPTRELEALADWLHAQFAADP
                     GLGPDDVLVVTPDIPTAAPLIANVFDSLPPERRIEWRISGLSGFEADKAAQAVLGLVE
                     LVTGRATLESFEAWIALPHVAKRFGFTSETVPMLGTWLRAAGYRYGLSDAHLRNLDPV
                     THANVRDMTLSRAVERLVMGFALPASEGDPLFDTLPVRGTEEDGWKDVSEEPGLLDAV
                     SRLAASLEGFRLRTEKAAAPEAWTTWLADATAVFFRDDEGGAFNRIRNAAATLREEIE
                     RAGVTEIPFGIFMKSLELAQETSPGGGMPTPRVTFTGMSQLRPLPYKIIAVVGLNEDC
                     AFPGNPHRQEFDLMAHAARRGDRDSRRDNRNVFLDILLAARRTLLISYVCGTGAGSAE
                     RQPSVVAQELREWLLTFAPGRAERRRAEALLTVNVPLNAYSTESFRDAGRGWRSHDPA
                     LFEALRKAVSAGYAATEQKFADTGLEVFRRTEVSVREICDFWKAPARKTLAAAGVRLP
                     DEAGDDERGMMPPDDGLSSWMRRNEALEAAASGDCANWLRRTACDPRFGAEGIRDWLS
                     EDAAAYAARVEELRIEAAVGRELPEEDLEIDLGEGFPRVTMRLRGVYESGDVRRFVRA
                     TVSKGTGNPVTLAFTEFIAACALERVDEGAIIAGWTKELGNGVKEPKDDTNPEKLVTI
                     AFKAYSADEARRILRALLRPCLAAAASAAQGVDTFGDYGEEDESADLILWRGGDLDRM
                     RRVADERRKMRFELFKKKLLKKTQDEKLVAAFEEKVDEALKAMAEPTQDDQQDD"
     gene            4019..7606
                     /locus_tag="ACZPB6_03445"
     CDS             4019..7606
                     /locus_tag="ACZPB6_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012789.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR1183561.1"
                     /translation="MTKDTNSRFEAVPPLSELNDGDAAAACDAVPEYCDDVPSCADVE
                     DCAAAQESCVPDDFFDDAPASEPEHEESLAVKSADSDGFVVEKADLTVPTLLEASAGT
                     GKTFSIKHLVLRLIVEQEIPINSLLVVTFTKAATAELKSRIREHLAETHSYLTGIYAA
                     EDVDPLVVRQVEIWRGMSISDEDAVKRLRESLARFDDSAINTIHGFCQKMLSEHVFTS
                     AGNFDTELSDDDAALRDEVTESFLRRELDACTSADERRLLMQGDDWAEKLRTLAGLPE
                     DLAKRVIPDDVEPLPREWLERFIGTAPAALRALKRARRLATYDDLLAEMWLTLRDDAT
                     GAFVAGIRNTYRGVLIDEFQDTDPVQFAIFRKLFLEIPKSERSADKPRALFFVGDPKQ
                     AIYSFRSADLDTYMRARKLISRHSRLGRNFRSCPKLVEAVNRFFSIAGPDAFLRHDLA
                     YKPVDAKVDRTGLFLLDEDGIWREADVFEIWYTEEGLEGKDAMNVATARSVADDIARL
                     ITLGRDGRAAIAAGPDDHVIGSVVIEVEDGETKKPVAKTIALRAVEARDIAILVQKRG
                     HVDNVKRALARRGVRVVMKSNDDVFRTEEAHDMLCLLHAFAEPGVERVLTALRATRLM
                     AATLSELCDQDEQARSDLRERLEAGVARWRRAGAAAALSAFIDEEHLAERLLPVEGGE
                     QRLTNYAHIIELLNEAGRKYATPAGLLSWFESARAQKGGETRNLRLASDANLVTVETI
                     HSSKGLQYPIVYLPSAESMAVMKGEKRSVFKQSDASGMELSITHGEVQESDALKVKRK
                     EELVRLAYVAMTRAACRLVLVVPQNRTSTGWHGNYRKNAYFMALTGSLEPDRDIVLDS
                     FRELGSLPGVRCVEIETLLTETADDITVAPPALDTDLGVDHAKPILPKWRVSSFSSIN
                     RSVTDDEVAWFGPKQSAGPLEGILAFPRGTKAGDAMHGMLEIADFSAVAPDTPEADAL
                     RRSIARSRIEQFLSFPDEASLDKAVGEAARMIYDVVNAEILPGIRLRDVKMTERASEM
                     PFLLRMRDGLSASDLKDTLERFGDMYAIPNLSDDDLSGFLTGFIDLAFGAKGRFWILD
                     WKSNAITRFVRTQADFTQHVMSDEMRVHRYRLQYLIYLVALRRFLKARLGRDYDDSLL
                     GGACYVFLRGVSADARRGPEGIQGVVYDPVGAERIARLDELFLPEWEQK"
     gene            7603..9570
                     /gene="recD"
                     /locus_tag="ACZPB6_03450"
     CDS             7603..9570
                     /gene="recD"
                     /locus_tag="ACZPB6_03450"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HIR1183562.1"
                     /translation="MTNITTNDPGRTLPPKLAEFGESLLRIADRAREAQGLEPFGEDM
                     HVFMRAVTSAFAGADDRGSVCVRLFDVARRMGLEVGEDEEAYDADIRAHLEELARWGL
                     AAAGAPAEGARPLAPLMLDEGAGGLASRLYFARFFAEEQRLAAALVRIASKPPRTLPE
                     VTASLVEKLTGALHADELQTKAVRLALENRLAVISGGPGTGKTTTVVLILECLLEENK
                     DLSIYLAAPTGKATSRMRQSIVAMTQGPIAGNFPRMKEVVSEETAQGAPRRVEEHTIH
                     KWLVKKTPSGEKPSADNPLPADVLIIDEASMVDIHLAARLFEVVSESTRVIILGDKHQ
                     LAAVGPGAVFADISATDGALAASTVDLKTSRRFAEGSVIAKLAAAINHDGGISSSTAC
                     TTVKRLLADAQTDGDPDWRIAWDDAMPQGEGGYGLAPAATAWLEERFSRYTAALIDYM
                     RVVTRPEPKADDLKAAGRALWEIFQTFRPLCAQRRGPQSVEGINAWAEAKVRTALAGA
                     DFKTFAEDRWNYPGRAVIIRSNDDILGVFNGDVGIVLPDEHGDLRAVFFDADRSMPPA
                     LMPAHDTAFAMTIHQSQGSEFPEVAVFLPTDPASGLATRELLYTGVTRTKRSVDIFGT
                     ERVLNASVENRTVRVSGLADRLAERAKAARG"
     gene            9783..10769
                     /locus_tag="ACZPB6_03455"
     CDS             9783..10769
                     /locus_tag="ACZPB6_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HIR1183563.1"
                     /translation="MAVGKKAVVLVVAAAVAAGAGWGFAEWQKSREAKPSVAWGSVDA
                     REVSLAFEGSGRIAELLREEGEAVKAGDVIGRLDTRALEIERRRAEAQAEGLDAQWRL
                     AEEGFRKEEISSAKAELDAVESNLALARKTEDRQLKLWKANATTAQNVDNARWSRIVL
                     EKQAASARAVYELKVAGLRPDEVRQTKAALDAGRAAVDSLTYQIETAAVLVSPVNGVV
                     RTRMAEPGDMASAARTVYQISIMDPKWVRAYVSETQLKYVQEGAEALVTTDTTEPLKA
                     RVGHISSEAEFTPKTVATQDLRTVLVYEVRLNVEDPGNRLRLGQPVTVDFAP"
     gene            10795..>11613
                     /locus_tag="ACZPB6_03460"
     CDS             10795..>11613
                     /locus_tag="ACZPB6_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1183564.1"
                     /translation="MTTSVIEASALCRVFASEDGREPVRAVDGVTFAVPPEARLVALI
                     GPDGAGKSTLMQMICGLVGQDSGSLSVLGRTPDPDDEDFVNAVGYMPQTLGLYKDLSV
                     MENMKLFGALRGAKPPEDMTIEAYYASLIALTGLEGFEGRPAGKLSGGMKQKLALAVA
                     LVRAPALLLLDEPTVGVDPLSRRELWAVIRRMVGQGGTTCFFSTAYLEEAESADWVLC
                     MSEGRIIGSGTPAELLGRTKGRTYALDLSGFSDAERKSLSRRMMLKVSPAPEKSV"
BASE COUNT         1978 a         3970 c         3768 g         1897 t
ORIGIN      
        1 aagacgaagc tccccgagac cttcacctcc tggcatgatc ccgacctcgt cattcccgcg
       61 acgcttcccg ccgacgtgct gcctggcttc ttcgtcctcg acggccacgg ccacctcgtg
      121 ggcttcgtgc gcggcttcgt tgactgggcc gatcccgccg tgcccgccta cttcgaaaag
      181 ctcgccgaca agtacgccgt tcgcaagtaa ctgaaaacgg aaggaacgga aggcgcggcc
      241 tttcgtttgc attttccccg ggccgcctgc cgctttcatg cgccggcggc ctcttcacat
      301 ccgcgaagca cgtccgtgtg cgtcacataa tgacgcacgg gcgcgtatcg tggcatgagg
      361 ggcgggtctt ttgtcaacac ccgctagaat gaactcattc ctgtttcaaa cgatcctcca
      421 gacatgatcc acaccgtcta cagcaacagc tacgaggtct tgcgcgtctg cctcatgaac
      481 aacatcgagg cgctcggcgt ctccacaggc gggtcgggcg gcgccagtct tttcgagcgc
      541 gccttcgaga aagtgcccgt catcacgccc aacaacgcgg ttgaggagga cctccgccgc
      601 gccgttgccg accgcgacgg catctgcgcg ggcatcgact tcatgaagct ctcctcctgg
      661 atgggcttct tctccaagga gcccatggcc aacatcgtcg gcaacgaggc cgactggatg
      721 atctgggacc tgttgcgccg cacggggtct gggagcttcc gcgagggccc gggtcgcgag
      781 cgcctgcgcc actatctcga gggcaggagc gacgaggacg tctggcatct ggcgaggcgc
      841 atcgccgagg tgttcgtcgt ctattcgacc tatcgtctcg actgggtcct cgactggctg
      901 ggcatgcatc ccgagatgct caacgacacg gacgcacgca cgacagagca gaaggtgctt
      961 gagcgccatc ccgactttgc atggcagcgc gacctctggc gcgagctcgc gagccgcccg
     1021 tcctggcgcg gacgccgctt cctcgagggc ttccccgaca tgctccgccg cctcgccgcg
     1081 agcggcggct ctaagcgcgt gaagctcgag aacgacttca ccgtgacgct tcccgatgcg
     1141 ctgcacgtgt tcgtgccttt cgtcgtgccg cccgtcatgc tgccgatcat caaggcctac
     1201 gcggcctcgg ggcgcgacgt ctggttctat cttctcaatc ccacgagcga atactggtac
     1261 gaccttctgc cgcgcaggct ctttgactgg aagaacgccc ccgagggcgc cgagctcgac
     1321 ggacatccgc ttcttgccga caacggcggg tcgacccgcg ccaacatcga ccgaatctgg
     1381 cgattcacgg ccgcgcccga cacggctgcg ggcctttcgg aactcggcgt gtccgaggag
     1441 ctcgatgagg acaccgtcag tcgtctgccg cagggaaccc gtaggttcct gtcgtcggac
     1501 ttcctcaagg gatatgcggg ccggcatcag gagatccgtg ccgacatggc cgttgccaac
     1561 gacagctact acatcgagcg gaacagcacc gatcttctct cccgcgtgca ggactcgatc
     1621 ctgaaccttg atgcggatct caagaagacg ggaggaggcg cgccgctctt tcgcgaggac
     1681 gaccgctcga tccgcttcat gcgctcgccc acgcccacgc gcgagctcga ggcgcttgcc
     1741 gactggctgc acgcgcagtt tgcggccgat cccggactcg gtcccgacga cgtgctcgtc
     1801 gtgacgcccg acatcccgac cgccgcgccg ctcatcgcca acgtcttcga cagcctgccg
     1861 cccgagcgcc gcatcgaatg gcgcatctcg ggcctttcgg gcttcgaggc cgacaaggcg
     1921 gctcaggccg tgctcggact cgtcgagctc gtcacgggcc gcgccacgct cgagtcgttc
     1981 gaggcttgga ttgcgctccc gcatgttgcg aagcgcttcg gcttcacgtc cgagacggtg
     2041 ccgatgctcg gcacctggct tcgcgccgcg ggctaccgct acggtctttc cgacgcgcac
     2101 ctgcgcaatc tcgatcccgt cacccatgcg aacgtcaggg acatgacgct ctcgcgcgcc
     2161 gtcgagcgcc tcgtgatggg ctttgcgctg cccgcctccg aaggcgatcc gctctttgac
     2221 acgctgcccg tcaggggcac cgaggaggac ggctggaagg acgtgagcga ggagcccgga
     2281 ctgctcgacg ccgtgagcag gctcgccgcc tcgctcgagg gcttcaggct gcgcaccgag
     2341 aaggctgcgg cccccgaggc ctggacgacg tggctcgctg acgcaaccgc cgttttcttc
     2401 agggatgacg agggcggggc cttcaaccgc atccgcaacg cggccgccac gttgcgcgag
     2461 gagatcgagc gcgcgggcgt gaccgaaatt ccgttcggca tcttcatgaa gagcctcgaa
     2521 cttgcccagg agacctcgcc cggcggcggc atgccgacgc cccgcgtcac cttcacgggc
     2581 atgtcgcagc tgcgtccgct tccctacaag atcatcgccg tcgtgggtct caacgaggac
     2641 tgcgccttcc ccggcaatcc gcaccgccag gagttcgacc tcatggcgca tgcagcccgc
     2701 cgcggggacc gcgacagccg ccgcgacaac cgcaacgtct tcctcgacat cctgctcgcc
     2761 gcgagaagga cgctcctcat ctcgtacgtc tgcggaacgg gtgcgggttc ggccgagcgc
     2821 cagccatccg tggtcgcgca ggagcttcga gaatggctgc tcacctttgc gccgggccgc
     2881 gccgaacgcc gccgcgcaga ggcgctcctc accgtcaacg tgccgctcaa cgcctattcg
     2941 accgagagct tccgcgatgc gggcaggggc tggcgaagcc atgatcccgc tcttttcgag
     3001 gcgctcagga aggccgtgtc ggcgggctac gccgcgaccg agcagaagtt cgccgatact
     3061 ggcctcgagg tcttcaggcg caccgaagtc tcggtgcgcg agatctgcga cttctggaag
     3121 gcgcctgcca ggaagacgct ggctgcggcc ggcgtcaggc ttccggacga ggcaggcgac
     3181 gacgagcgcg gcatgatgcc gcccgatgac ggactctcga gctggatgcg caggaacgag
     3241 gccctcgagg ccgctgcgtc gggcgactgc gcgaactggc tcaggcgcac cgcctgcgat
     3301 ccgaggttcg gagccgaggg cattcgcgac tggctcagcg aggacgcggc cgcctatgcg
     3361 gccagggtcg aggagctcag gatcgaggcc gccgtcggca gggagctgcc cgaggaggat
     3421 ctcgagatcg acctcggcga gggcttcccc cgcgtgacga tgcgcctcag gggcgtgtac
     3481 gagtccggcg acgtgcgccg cttcgtgcgc gcgacggttt cgaagggcac gggcaacccc
     3541 gtgacgctgg ccttcaccga attcatcgcg gcttgcgcgc ttgagcgcgt cgacgagggc
     3601 gcaatcatcg cgggctggac caaagagctc ggcaacggcg tgaaggagcc caaggacgac
     3661 accaatcccg agaagctcgt gacgatcgcc ttcaaggcct attcggccga tgaggcgcgc
     3721 aggatcctcc gcgccctgct caggccctgt ctcgctgcgg cggcttcggc cgcccagggc
     3781 gtcgacacgt tcggtgatta tggggaggag gacgagtccg ccgacctcat tctctggcgc
     3841 ggaggcgact tagacaggat gcgcagggtt gcggacgaaa ggcgcaagat gcgcttcgag
     3901 cttttcaaga agaagctgct caagaagaca caggacgaaa aactggtggc cgcttttgaa
     3961 gaaaaggtgg acgaggccct caaggccatg gctgaaccga cgcaggatga ccagcaagat
     4021 gactaaagac acgaattccc gtttcgaagc ggtgccgccg ctttcggaac tcaatgacgg
     4081 cgacgctgcg gccgcctgcg acgccgtgcc cgagtactgc gacgatgtgc cgtcgtgtgc
     4141 ggacgtcgag gactgcgcgg ctgcgcagga atcctgcgtc cccgacgatt tcttcgacga
     4201 cgctcccgcg tccgaacctg agcatgagga gtccctcgcc gtgaagtccg ccgacagcga
     4261 cggcttcgtg gtggagaagg ccgacctcac cgttccgacg cttcttgagg cgtctgcggg
     4321 cacgggcaag accttctcga tcaagcatct ggtgctcagg ctgatcgtcg agcaggagat
     4381 cccgatcaac agtcttctcg tcgtgacctt cacgaaggcg gccacggcgg agctcaagag
     4441 ccgcatccgc gagcatcttg ccgagacgca cagctatctg acgggcatct acgccgccga
     4501 ggacgtcgat cccctcgtcg tgaggcaggt ggagatctgg cgtggcatgt ccatcagcga
     4561 cgaggatgcc gtcaagcgcc tgcgcgaatc gcttgcgcgc tttgacgact cggccatcaa
     4621 cacgattcac ggcttctgcc agaagatgct ctccgaacac gtcttcacgt cggcggggaa
     4681 cttcgatacg gagctctcgg acgacgacgc agccctgcgc gacgaggtga cggagtcctt
     4741 cctgcgccgc gaactcgacg cctgcacgtc ggccgacgag cgtcgcctgc ttatgcaggg
     4801 cgacgactgg gcggaaaagc tcagaacgct ggccggcctg cccgaagatc tggcaaagcg
     4861 cgtcattccc gacgatgtcg agccgctgcc ccgcgaatgg ctcgagcgct tcatcggcac
     4921 cgcgcctgcg gcgcttcgcg ccctcaagcg cgccaggagg ctcgcgacct acgacgacct
     4981 gctcgccgag atgtggctca cgctgcgcga cgatgccacg ggcgcctttg tggccggcat
     5041 caggaacact tacaggggcg tgctgatcga cgagttccag gacacggacc ccgtgcagtt
     5101 tgccatcttc aggaagctct tcctcgagat cccgaagagc gagcgctcgg cggacaagcc
     5161 ccgcgcgctc ttcttcgtgg gcgacccgaa gcaggccatc tacagcttcc gctcggccga
     5221 cctcgacacc tacatgcgtg cgcgcaagct catcagtcgc cacagccgcc tcggacgcaa
     5281 cttccgctcc tgcccgaagc tcgtcgaggc ggtcaaccgg ttcttctcga tcgcgggccc
     5341 cgacgccttc ctgcgccacg acctcgccta caagcccgtc gatgcgaagg tcgaccgcac
     5401 gggcctcttc ctgctcgacg aggatgggat ctggcgcgag gcggacgtat ttgaaatctg
     5461 gtacaccgag gaagggcttg agggcaagga cgcgatgaac gttgcgacgg cccgctctgt
     5521 tgccgacgac atcgcccgac tcatcacgct cgggcgcgac ggcagggctg cgatcgccgc
     5581 aggtcccgac gaccatgtga tcggctcggt cgtgatcgaa gtcgaggacg gcgagacgaa
     5641 gaagcccgtg gcgaagacca ttgctcttcg cgccgtcgag gcccgcgaca ttgcgatcct
     5701 cgtgcagaag cgcggtcacg tcgacaatgt gaagcgggcg ctcgcccgcc gaggcgtgcg
     5761 cgtcgtcatg aagagcaacg acgacgtctt caggaccgag gaggcgcacg acatgctctg
     5821 cctgctgcac gccttcgccg agcccggcgt cgagcgcgtg ctcacggcgc tcagggcgac
     5881 ccgtctcatg gccgccacgc tctccgagct ctgcgaccag gacgagcagg cgcgctcgga
     5941 ccttcgcgag cgccttgagg cgggcgtcgc ccgctggcgt cgtgcgggtg cggcggctgc
     6001 gctctcggcc ttcatcgacg aggagcacct cgccgagcgc ctgttgcccg tcgagggcgg
     6061 cgagcagcgc ctcacgaact acgcgcacat catcgagctt ctcaacgagg cgggcaggaa
     6121 gtatgcgacg cctgcaggcc tgctgagctg gttcgagtcc gccagggcgc aaaagggcgg
     6181 cgagacgcgc aacctgcgcc ttgcctccga cgcgaacctc gtgacggtcg agaccattca
     6241 ctcgagcaag ggcctgcagt atccgatcgt ctatctgccg agtgccgagt ccatggccgt
     6301 catgaagggg gagaagcgct ccgtcttcaa gcagagcgac gcttcgggca tggagctctc
     6361 gatcacccac ggggaggtgc aggagtccga tgcgctcaag gtcaagcgca aggaggagct
     6421 cgtgcgcctt gcctacgtcg ccatgacccg tgcggcgtgc cggctcgtgc tcgtggtgcc
     6481 acaaaacaga acgtctacgg gctggcacgg gaattacagg aagaacgcct acttcatggc
     6541 gctcacgggc tcgctcgagc cggatcgcga catcgtgctc gacagtttcc gggagctcgg
     6601 cagcctgccc ggcgtgcgct gcgtcgagat cgagacgctc cttacggaga cggccgacga
     6661 cattacggtc gcgccgcccg cgctcgatac cgatctcggc gtcgatcacg cgaagcccat
     6721 cctgccgaag tggcgcgtga gctcgttctc aagcatcaac cgctccgtca ccgacgacga
     6781 agtcgcctgg ttcggtccaa agcagtcggc cggtccgctc gagggcattc tggccttccc
     6841 gcgcggcacg aaggcgggtg acgcgatgca cggcatgctt gagatcgccg acttttccgc
     6901 agttgcgccc gatacgcccg aggccgatgc gctgcgccgc agcatcgccc gcagccggat
     6961 cgagcagttc ctctccttcc ccgacgaggc ctcgctcgac aaggccgtcg gagaggccgc
     7021 gcgcatgatc tacgacgtcg tcaacgccga gatcctgccc ggcatccgcc tgcgcgacgt
     7081 gaagatgacg gagcgagcct ccgaaatgcc gttcctcctg cgcatgcggg acggactcag
     7141 cgcgtcggac ctcaaggata cgcttgagag gttcggggat atgtacgcga ttccgaacct
     7201 ctcggacgac gacctctcgg gcttcctcac gggcttcatc gaccttgcgt tcggcgcgaa
     7261 gggcaggttc tggattctcg actggaagag caatgcgatc acccgcttcg tgaggacgca
     7321 ggccgacttc acgcagcatg tcatgtccga cgagatgcgc gtgcaccgct accgtctcca
     7381 gtacctcatc taccttgtgg cgctcaggcg cttcctcaag gcgaggctcg ggcgcgacta
     7441 cgacgactca ctcctcggtg gcgcctgcta cgtgttcctg cgcggcgtca gcgccgatgc
     7501 ccggcgaggc cccgaaggca tccagggcgt cgtatacgac cccgtgggcg ccgaaaggat
     7561 cgcccgcctt gacgaactct tcctgcctga atgggagcag aaatgacgaa catcaccacc
     7621 aacgatcccg gccgcacact cccgcccaag cttgcggagt tcggcgagtc gcttcttcgc
     7681 atcgccgacc gtgcgcgcga ggcgcagggg ctcgagccct tcggcgagga catgcacgtc
     7741 ttcatgcgtg ccgtcacgtc cgccttcgcg ggcgccgacg accgcggcag cgtctgcgta
     7801 aggcttttcg acgttgcgcg ccgcatgggc cttgaggtcg gggaggacga ggaggcgtac
     7861 gacgcggaca tccgcgcgca tctcgaagag ctcgcccgct gggggcttgc cgctgcgggt
     7921 gcacccgcag agggcgcgcg ccccttggcg cccctcatgc tcgacgaagg cgcaggcggg
     7981 cttgcctccc gactttattt tgcgaggttc ttcgccgagg agcagcgcct tgcggctgcg
     8041 ctcgtgcgga tcgcctcgaa gcctccgcgc acgcttcccg aggtgacggc ctcgctcgtc
     8101 gagaagctca cgggcgccct gcacgccgac gaactccaga ccaaggccgt gaggcttgcg
     8161 ctcgagaacc gccttgcggt gatttcgggc ggtcccggca ctggcaagac gaccaccgtg
     8221 gttctcatcc tcgagtgtct gctcgaggag aacaaggacc tttccatcta tcttgccgct
     8281 ccgacgggca aggccacgag tcgcatgcgc cagtcgatcg tcgcgatgac ccagggcccg
     8341 atcgcgggca atttcccgcg catgaaggag gtcgtttccg aggagacggc tcagggcgcc
     8401 ccgcgacgcg tcgaggagca cacgatccac aagtggctcg tcaagaagac gccttcgggc
     8461 gagaagccct cggcggacaa tccgcttccc gccgacgtgc tcatcatcga cgaggcctcg
     8521 atggtcgaca tccatctggc ggcccgcctc ttcgaggtgg tctcggagag tacgcgcgtg
     8581 atcatccttg gcgacaagca ccagctcgcc gccgtgggac cgggcgccgt ctttgcggac
     8641 atctccgcca ccgacggcgc gcttgccgca agcacggtcg acctcaagac gagccgccgc
     8701 ttcgccgaag gctccgtcat cgccaagctt gccgccgcga tcaatcacga cggcgggatt
     8761 tcgagctcca ccgcctgcac gacggtcaag cgcctgctgg ccgacgcgca gaccgacggc
     8821 gaccctgact ggcgcatcgc ctgggacgac gccatgccgc agggcgaagg cggttacggc
     8881 ctggcgcccg ctgccaccgc gtggctcgag gaacgcttct cgcgctacac ggccgcgctc
     8941 atcgactaca tgcgcgtcgt cacgcgcccc gagccgaagg ccgacgacct gaaggccgcg
     9001 ggccgcgccc tctgggagat cttccagacc ttcaggccgc tgtgtgccca gcgccgcggc
     9061 ccgcagagcg tcgagggcat caacgcctgg gccgaggcta aggtcaggac ggcgctcgcc
     9121 ggcgcagact tcaaaacctt cgccgaggac cgctggaact atcccggccg cgccgtcatc
     9181 atccgaagca acgacgacat cctcggcgtc tttaacggcg acgtcggcat cgtgctcccc
     9241 gacgagcacg gcgacctgcg cgccgtcttc ttcgacgccg accgcagcat gccgcccgcg
     9301 ctcatgcctg cgcacgacac ggccttcgcc atgacgatcc accagtccca ggggtccgaa
     9361 ttccccgagg tcgccgtctt cctgccgacc gaccccgcct cgggcctcgc cacccgcgag
     9421 ctcctctaca cgggcgtcac ccgcaccaag cgctccgtcg acatctttgg cacggagcgg
     9481 gtcctcaacg cctccgtcga gaaccgcacc gtgcgcgtct cgggcctcgc cgaccgcctc
     9541 gccgagcgcg cgaaggccgc ccgcggctga ccttcccgca ccgtcaccag aacctcgaaa
     9601 aggcctctcc ccgcgtccga ataggggccg ggaggggcct tttgcaccct cattgtgaaa
     9661 tggccggtca gtacaaaaca aaggtgaccg acgggttcaa aatgggctgg aacggtgtta
     9721 tagtctcctt aagaaaatga accgggcgat tcaaaatgaa tccgccctca aggaggcttg
     9781 aaatggcggt tggaaagaag gccgtggtgc tggtcgttgc ggcggcggtc gcggcgggag
     9841 caggctgggg cttcgcagag tggcagaaga gccgcgaggc gaagccgagc gtggcctggg
     9901 gcagcgtgga tgcgcgcgag gtgtccctgg ccttcgaggg gagcgggcgc atcgccgagc
     9961 ttctcaggga ggaaggcgag gccgtgaagg cgggcgacgt gatcgggcgc ctcgacacgc
    10021 gtgcgctcga gatcgagcgc cgtcgcgcag aagcgcaggc cgaagggctt gacgcccagt
    10081 ggcgtctggc cgaagagggc ttccgcaagg aggagatcag ttccgcgaag gccgagctcg
    10141 atgcggtgga gagcaatctt gcgcttgcaa gaaagaccga ggaccgccag ctcaagctct
    10201 ggaaggccaa cgccacgacg gctcagaacg tggacaacgc gcgctggagc cgcattgtgc
    10261 ttgaaaagca ggctgcgagc gcccgtgcgg tctacgaact caaggtcgcg ggtctgcgtc
    10321 ccgacgaggt ccgccaaacg aaggccgccc ttgatgcggg ccgcgccgcc gtcgactcgc
    10381 tcacctatca gatcgagacg gccgccgttc tcgtctcgcc cgtcaacggc gtcgtgcgca
    10441 cgcgcatggc cgagccgggc gacatggcct ccgccgcgag gaccgtctat cagatcagca
    10501 tcatggatcc gaagtgggtc cgcgcctacg tttccgagac ccagctcaag tacgtgcagg
    10561 aaggcgccga ggcgctcgtg acgaccgaca cgaccgagcc gctcaaggcc cgcgtgggcc
    10621 acatctcgag cgaggccgag ttcacgccca agaccgttgc gacgcaggac ctgcgcacgg
    10681 tgctcgtcta cgaggtgagg ctcaacgtcg aggatccggg caaccgcctg cgtctcggcc
    10741 agcccgtgac cgtggacttc gcgccctgaa tccgccgctg gaggaaagca cgtcatgacg
    10801 acatccgtca ttgaagcgtc ggcgctctgc agggtctttg cgtccgagga cggacgcgag
    10861 cccgtgcgtg cggtcgacgg cgtcaccttc gccgtaccgc ccgaggcgcg cttggtcgcg
    10921 ctcatcggtc ccgacggcgc gggcaagtcg acgctcatgc agatgatctg cgggctcgtg
    10981 ggccaggact caggctcgct cagcgtgctc gggcgcactc cggaccccga cgacgaggac
    11041 ttcgtcaacg ccgtgggcta catgccgcag acgctcggcc tctacaagga cctctccgtc
    11101 atggagaaca tgaagctctt cggtgcgctc aggggcgcga agcctcccga agacatgacg
    11161 atcgaggcgt actacgcgag cctcattgcg ctcacgggcc ttgagggctt cgagggccgc
    11221 cctgcgggca agctctcggg cggcatgaag cagaagctcg cgctggccgt cgcgctcgtg
    11281 cgcgcgcccg cgctccttct cctcgacgag cccaccgtgg gcgtcgaccc gctctcgcgc
    11341 cgcgagctct gggccgtgat ccggcgcatg gtcgggcagg gcggcacgac gtgcttcttc
    11401 tccaccgcct atctcgagga ggctgagtcg gccgactggg tgctctgcat gtcggagggt
    11461 cgcatcatcg gctcgggcac gcccgcggag cttctcgggc gcacgaaggg ccgcacctat
    11521 gcgctcgatc tttcgggttt ttcagacgcc gagaggaagt cgctctcgcg ccgcatgatg
    11581 ctcaaggttt cgcccgcccc cgaaaagtcg gtc
//
LOCUS       DBMDTI010000040        11535 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1674_length_11535_cov_2.244446, whole genome shotgun sequence.
ACCESSION   DBMDTI010000040 DBMDTI010000000
VERSION     DBMDTI010000040.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 11535)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11535)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11535
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1674_length_11535_cov_2.244446"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="ACZPB6_03465"
     CDS             <1..677
                     /locus_tag="ACZPB6_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1183565.1"
                     /translation="SATAKQPHLWLTPRGERFCDEKIMLEWPFAGNALERIGGTMYVV
                     YDQATLDHMEKDKGIDLGVGVMVPVGTKLTNFEKEWADAVKGGWAFKADTLEGLAKAT
                     GMDPKKLVKTVEAYNGFCAVRHDAEFAKDPAYLREVKTGPFYAIKSVASSLGTLGGIK
                     ANERFEVVTQDETPIRGLYAAGNDVGGMYGDSYDLLMAGSTVGFAVNSARIAVESADA
                     YIKETK"
     gene            complement(769..1491)
                     /locus_tag="ACZPB6_03470"
     CDS             complement(769..1491)
                     /locus_tag="ACZPB6_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024934.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1183566.1"
                     /translation="MRQIFLALPYVCPREPARIADLIREHGTLRDVAAGEVLKRGGDA
                     NRLFLLEEGICVYYVAGETTGHSTIMSLLLPGTTLCDMTSSVGRRCNVETRAVQASRV
                     WCTAPDFYERHVFTNPELAVEKYQHAVGKQEATLEGMIANFTLPPEQRVKILLKAVLL
                     HEQAELGSDWTRIPYDLPAEVIGQTVNLTRQTVAQTIGDWRRAGLARRVGRHLEVLPT
                     LFDDIYDWIEHRGGCLPDWHTG"
     gene            complement(1649..2812)
                     /locus_tag="ACZPB6_03475"
     CDS             complement(1649..2812)
                     /locus_tag="ACZPB6_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013183.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR1183567.1"
                     /translation="MSEFFILKPFERKRLCYGRFQSATRPLEWARGATLAVDVVAASD
                     IPPYDLAMRDGWAVSSLDDHRDIDEVSLLNGTLPEPLKRGRARWINTGGMIPIGCDAV
                     IAAASPDDRAAARQAVASERHVLRRGADRRRGEVILRKGTRLGVRETALLSEACIEEV
                     QVSRTPRILILATGSEITMGPKNPVACRRASNAVYLQALLDAAGIEQSRIVRLPDDAD
                     EIARALERRVGIDLIVSIGGTGRGTHDLTRRAIAAAGGTVIEGTGDVKGAPPFVLADL
                     GGTPLVGLPGNPLGAVMIIQRVLLPMLEEDFGLTLPRREVVDATLACDADIDAEGDLC
                     VTLDRNEEGRLVATPVRKGSCRTRLFAVEAGTVSVKPCCLRAGETVPVERFFN"
     gene            3178..3882
                     /locus_tag="ACZPB6_03480"
     CDS             3178..3882
                     /locus_tag="ACZPB6_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1183568.1"
                     /translation="MELQDFLSKLSLPQDFTRRGFLKATSVMAAIGMMNQARAASGVR
                     PYIVLETAKGILICDPNLCVACHRCELACTEFNEGKADPRLARIKITRNVMFGHAGTN
                     VYPPTKGIWGDGIVVQDTCRQCAHPTPCANACPQGAIVVDPKTGARRVDKEKCIGCHL
                     CEKACPWGMMTFDVEEKKASKCFLCDGEPKCVKHCPAAAIRYVPWMDRTGDEPRQSIH
                     GYLPQENRDQCSICHR"
     gene            3899..6010
                     /locus_tag="ACZPB6_03485"
     CDS             3899..6010
                     /locus_tag="ACZPB6_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR1183569.1"
                     /translation="MSENKKYGYTGKGLRVDLTTGKITVEPTFPRFDGYIGGTAFGYK
                     VFWDEVPPETDCYAPENKLVIAPGPLSGTGAICSGRTAITTLWPTAWPRSLIASAHVG
                     GELAHKLKFAGWDFVIIEGEAKSPVYLYIHNEKVEIRDASAAWGQGTRRSAEVLAKET
                     TPEASFGVIGQAGENLVPMSNMMVDKSHSAGGLGSVMGKKHLKGIVVYGNQAIHIHAD
                     PKEWEELIDSHRKILGAHTHSIVSRKPHPMFEYHTPASRWSAFPGAKWGAANPPVMLT
                     DADMDPHNLNHIGYRTCSSEFYMGPTMWNYTVRNTGCYACPIRCYSVIRDDETAAKYN
                     VNAITEQTCMALYGRWFFPSLLKDLMKPVSREACLVGSQTMDDLGLWCNYGQLHRDFC
                     TMYTKGYFKKYLPEEEYKSIDWSKIDNPDPRILQDILPRIAYRKGEFGRWMGETTPVM
                     LEHFGLTEDEWKKNHDTLYWSVGHPKHHGNENDGQIGTVLNCMYNRDPMSHGHINFST
                     SGLPLELQREIAAKFWGDGSAVDGIGDYRPTNKYKMIRLRWVIARKELHDMLGICSWT
                     APWELSPLRERGYIGDIEMESKVFKAVTGISMTQDELDKAGLRAFLLQRLYTMRQLKT
                     KDMRHVHDRYPDWIFDDAKGRAPFTKGTIRMEHDDIEKSFDLFFELMDFDVKTGAPTE
                     KCLNEYGLAKAVPVMKKEGLL"
     gene            6035..6940
                     /locus_tag="ACZPB6_03490"
     CDS             6035..6940
                     /locus_tag="ACZPB6_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183570.1"
                     /translation="MQENMNPAPEGAQTPADEEAAREAARLSQLKADMLVLIDTVADS
                     SRDVKLIDGDTLFERFAKRASVAEAPEVDELVREAEADIDAVLGRTDGVSANVVKFRS
                     KSEMKAHADEVATQKAQRRVFSRREVIQNLLAGNVASVVKAAEAADAKTEPEGEAREI
                     TREYFDEVLSRVLEGDYGVCSLESWDKVTYYHYRPLLSCSYARILSAENNPVEQVVDM
                     IRESSRVYPRPVAIGVFEEPPFGIEAEELQEMLKMIAQDPEKQDIRFTTSSIGTVFLY
                     SNKYLEDGYAEFLAEEQDVGAVMNP"
     gene            6968..7963
                     /locus_tag="ACZPB6_03495"
     CDS             6968..7963
                     /locus_tag="ACZPB6_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR1183571.1"
                     /translation="MKFQMKSTAKAVALGIVTLAASAAFATQTPVEFDLKGHDEFVKS
                     LGKDAIAPQQRTAEESKAILAKVYNEKLEQHVSSFHYEKGVTCISCHDQQKQGGPDWM
                     VPVTNPPMKKECGDCHETQAYVVSHTKSHSKYECTVCHMPNTAAGPNDGSNGKMDAVR
                     RLHTYKINVSPDASTWTKDKDGAWVLSKDEDGHGYVDLMWSCARNAPADYTVAEGRGC
                     HSKATSELDPGLVYQDEKEVYGEVMKWQKPVKDGYEQIKGGVERVSKLLEVTALPYEK
                     QTQIRLLLDKATEVSDLIEKDGSWGAHASHYLQDRVKSALSYIDQAQTLLDKGGY"
     gene            8064..8789
                     /locus_tag="ACZPB6_03500"
     CDS             8064..8789
                     /locus_tag="ACZPB6_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1183572.1"
                     /translation="MTTKITRRALLGAAGAGVVTLGSAEAAALAASSEASGSREPHYA
                     MVFDQNKCVGCGDCKAACNEANHLPPGISRLLMERQSKSHRVIGKEPCPICGKLEECD
                     CERKYVRVSCQQCQDAPCVTVCPTGAAHHDPETGIVTMDPDRCVGCKYCIVACPYNAR
                     FINAETKVADNCDFCLHTRFAEGRKPACVESCKYDALVFGNVNDPYSYISRLIRAKDT
                     VRIRPELGTRPSLRYIPFVKKGA"
     gene            8791..9717
                     /gene="nrfD"
                     /locus_tag="ACZPB6_03505"
     CDS             8791..9717
                     /gene="nrfD"
                     /locus_tag="ACZPB6_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR1183573.1"
                     /translation="MDMNFAIGLSQGIAWPWPIAVYLFLAGISGGAVAAAVILNLYRK
                     QAHENTPVLKAASVIGAATILLGMVCLVLDLTNPFNFWRILIYYNPTSVMSLGVMALL
                     VYIPTVCILTLVALRGLLPKVGLGFLDGIVGFFDGWRVVLQWIALVFALTICAYTGFL
                     ISALIRFPLINQAVLPALFVASGLSAGMAATKAFACLAFGDRHESCDQHLLHAAEWPV
                     MAAEALCIFMIAVALVNGNAAAQAAFAAFTTGAWAIEFWVGVVCVGFCGPLVLGLIGG
                     GLMSFYLSALCSVAGMMCLRLFILYAGQLHGL"
     gene            9795..10628
                     /gene="fdhD"
                     /locus_tag="ACZPB6_03510"
     CDS             9795..10628
                     /gene="fdhD"
                     /locus_tag="ACZPB6_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR1183574.1"
                     /translation="MPCLPNDFPGAVSTVPVLKNFMSLETEDVVAEEVPVALVYNGIS
                     HTVMMASPVMLEEFGTGFSLTEGIVESVSDIYDVEVIEACGSGREVRITISSEAFHNF
                     KARRRAMLGRTGCGICGVESLGDALRPVPDLPATQTFDMRFYRDAVAQLKRFERIGAL
                     TGCTHASVWIRPDGTPAGGAEDVGRHVALDKLLGLRAREGWRDGALVVSSRASYEMVQ
                     KAAMCGVEILFAVSAPTALAVRTAERCGMTLAAFARAGGPNVYTHPERLVGAQDERLC
                     A"
     gene            10829..11302
                     /locus_tag="ACZPB6_03515"
     CDS             10829..11302
                     /locus_tag="ACZPB6_03515"
                     /inference="COORDINATES: protein motif:HMM:NF023742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1183575.1"
                     /translation="MRPRKGRCRIEVTKANIAYKFELMPTREQKRIFAQAAGCRRFVW
                     NKAIELQKRCLEQEVPFLKYTELAGRLVLWKKEDDMLWLQEAPSQALQQCRFPVTGRR
                     LDADDKRNGGLPSGSPPLLLVGPASLREETGERGFRTCRGRSRCRCRRGGRRHSP"
     gene            complement(11238..>11535)
                     /locus_tag="ACZPB6_03520"
     CDS             complement(11238..>11535)
                     /locus_tag="ACZPB6_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HIR1183576.1"
                     /translation="THAVRLGARLEGAFQTFDWYAGLAYEHVFDGKAEGELKFAGATA
                     ALDAPSLKGDSAIVDLGFTMKPEANGPWTIGVGLKGYAGDRRGGTGSVNVLYTF"
BASE COUNT         2199 a         3748 c         3598 g         1990 t
ORIGIN      
        1 tctccgcaac cgccaagcag ccgcacctgt ggctcacgcc caggggcgag cgcttctgcg
       61 acgagaagat catgcttgag tggccgtttg cgggcaatgc tctcgaacgc atcggcggca
      121 cgatgtacgt cgtgtacgat caggccacgc tcgatcacat ggaaaaggac aagggcattg
      181 atctcggtgt cggtgtgatg gttcccgtcg gcacgaagct cacgaacttc gagaaggaat
      241 gggctgatgc ggtcaagggc ggctgggcct tcaaggccga cacgctcgag ggccttgcca
      301 aggccaccgg catggatccc aagaagctcg tgaagaccgt cgaggcctac aacggcttct
      361 gcgccgtgcg tcatgacgcc gaatttgcaa aggatccggc ctacctgcgc gaagtgaaga
      421 cgggtccctt ctacgcaatc aagtcggtcg cttcgtcgct cggcacgctc ggcggcatca
      481 aggccaatga acgctttgaa gtcgtgacgc aggacgaaac gccgatccgc gggctctatg
      541 ccgcaggcaa tgacgtgggc ggcatgtacg gcgactccta cgacctgctg atggcgggct
      601 ccaccgtcgg cttcgcggtc aactcggccc gcattgcggt cgaatcggcc gatgcctaca
      661 tcaaggagac gaaataaggc tcagagaggt cttttgaacg gggcctcatg acatgagggg
      721 cggcggtctt cggaccgccg ccccttgctg attatggcct gtggtgcctc agcccgtgtg
      781 ccagtccggc aggcagccgc cgcgatgctc gatccagtcg tagatgtcgt cgaagagcgt
      841 gggaagaacc tcgagatggc gaccgacgcg gcgggcaagt cccgcacggc gccagtctcc
      901 gattgtctgt gcgaccgtct ggcgcgtgag gttgacggtc tgcccgatga cttctgcggg
      961 aagatcgtag ggaatccgcg tccagtcgga gccgagctca gcctgttcgt gaaggagcac
     1021 ggccttgagg aggatcttga cgcgctgctc gggcgggagc gtgaagttcg cgatcatgcc
     1081 ttcgagcgtt gcctcctgct tgccgacggc gtgctggtac ttttcgacgg cgagttcggg
     1141 atttgtgaag acgtgccttt catagaagtc tggcgccgtg caccagaccc tggaggcctg
     1201 gacggcccgc gtctcgacgt tgcagcggcg gccgacgctc gaggtcatgt cgcagagcgt
     1261 ggtgccggga agcaggagcg acatgatcgt cgagtgcccc gtcgtttctc ccgccacata
     1321 gtagacgcag attccctctt caagcagaaa gagcctgttc gcatcgccgc cgcgcttgag
     1381 aacctcgcct gccgccacat cgcgaagcgt cccatgctcg cggatgaggt ccgcaatgcg
     1441 cgctggttct cttgggcaga cataaggaag ggcaagaaag atctgtctca tgggcggtcg
     1501 tcgtcggttt caatggtcct gaaaaaagta tagggcagag cgagtcgcag ggaggaggcg
     1561 cggcggaagg agagggggtg gtaaaaaagg agcgccgcag ggagaggtcc cgtgcggcgc
     1621 atgcttttct gttcggatgc ggtttcgctt agttgaagaa ccgctcgacc ggtacagtct
     1681 cacccgccct gaggcagcaa ggcttcacgc tgaccgtccc cgcctcgacg gcgaagagtc
     1741 ttgtgcggca gcttcccttt cggacgggcg tggcgacgag gcgcccctcc tcattccggt
     1801 cgagcgtcac gcagaggtcg ccctcggcgt cgatgtcggc gtcgcacgca agcgttgcat
     1861 cgaccacttc cctgcggggg agcgtgaggc cgaagtcctc ctcgagcatg gggaggagca
     1921 cgcgctggat gatcatgacg gctccgagag gattgccggg aagtcccacg agcggcgtgc
     1981 cgcccaggtc ggcgagcacg aagggcgggg cgcccttcac gtcgcccgtg ccctcgatga
     2041 ccgtgccgcc cgcagctgca atcgccctgc gggtgaggtc gtgggtgccg cggcccgtgc
     2101 cgccgatgct cacgatgagg tcgatcccga cacgtctttc gagcgcgcgg gcgatctcgt
     2161 ccgcgtcgtc ggggaggcgg acgatgcggc tctgttcgat ccctgcggcg tcgagaagcg
     2221 cctgcagata caccgcattg ctcgcgcggc ggcaggcgac gggattcttc gggcccatgg
     2281 tgatctcgct ccccgtcgcg aggatcagga ttctcggcgt gcgcgatacc tgcacctcct
     2341 cgatgcacgc ttcgctcaaa agcgccgtct cgcgaacgcc gagcctcgtt cccttcctga
     2401 ggatcacctc gcctcgcctt ctgtcagcgc cgcgtcggag aacgtgtcgc tctgaggcaa
     2461 cggcctgccg ggcggccgca cgatcgtcgg gactcgcggc ggcgataacg gcatcacatc
     2521 caatgggtat cattccgccc gtattgatcc atcgcgcgcg tcctcttttc aggggttcag
     2581 ggagggtgcc gtttaaaagt gacacttcgt caatatctcg atgatcgtca agggatgaaa
     2641 ccgcccagcc gtcgcgcatg gcgaggtcgt atggcggtat gtccgacgcc gcgacgacgt
     2701 cgacggcgag cgttgcgccc cgcgcccatt caaggggacg ggttgccgat tgaaaacgcc
     2761 cgtagcagag tcgttttcgc tcgaagggct tcaggatgaa gaattccgac atggtgtggt
     2821 ccgggggctt gaccgtgtgt tgtgctgaga atccgaaggc ctgagaccct cgggggcatc
     2881 ctaattatgt atatgaacag ccaaagggat gtgtaaggat tactactttc ggatgactcg
     2941 tgtcaaaagg tcggtttgtt gactattgaa tacaggctta agagccgcat aattcccacc
     3001 gtcagaagca ccaacgagcc ccccaaaaaa tgctgatttc tcatcccgat gaggactccg
     3061 cgacttgttt gtgatctccg acaacgaccg gctgcccgac agggcgccgc cggtccggcc
     3121 cctcgcaccg gaacacccgg accggtcttt tgtaaaacaa caatcaggag aaacaccatg
     3181 gagcttcagg atttcttgtc caagctcagc ctgccgcagg acttcacgcg ccgcggcttt
     3241 cttaaggcta cgtccgtcat ggctgcgatc ggcatgatga accaggcgcg cgccgcgagc
     3301 ggcgtgcgtc cctacatcgt gcttgagacc gccaagggca tcctcatctg cgacccgaac
     3361 ctctgcgttg catgccaccg ctgcgagctc gcatgcaccg agttcaacga aggcaaggcc
     3421 gacccgcgtc tggcccgcat caagatcacg cgcaacgtca tgttcggcca tgccggcacg
     3481 aacgtctatc cgccgacgaa gggcatctgg ggcgacggca tcgtcgttca ggacacctgc
     3541 cgccagtgcg cccatccgac gccgtgcgcc aacgcctgtc cgcagggcgc catcgtggtc
     3601 gatccgaaga cgggcgcccg ccgcgtcgac aaggaaaagt gcatcggctg ccacctctgc
     3661 gaaaaggcct gcccgtgggg catgatgacc ttcgacgtcg aggagaagaa ggccagcaag
     3721 tgcttcctct gcgacgggga gcccaagtgc gtcaagcact gtccggccgc cgccatccgc
     3781 tacgtgccct ggatggaccg cacgggtgat gaaccgcgcc agagcatcca cggctacctg
     3841 ccgcaggaaa accgcgacca gtgcagcatc tgccatcgtt gattagagga agctagaaat
     3901 gtctgaaaac aagaaatacg gctacaccgg caagggtctg cgcgttgacc tcacgaccgg
     3961 caagatcacg gtcgagccga ccttcccgcg ttttgacggc tacatcggcg gcaccgcctt
     4021 cggctacaag gtcttctggg acgaagttcc gcccgaaacc gactgctacg cacctgaaaa
     4081 caagctcgtg atcgctccgg gcccgctctc gggcaccggc gccatctgct ccggccgcac
     4141 cgccatcacg acgctctggc cgaccgcctg gccgcgctct ctgatcgcct ccgcccacgt
     4201 cggtggcgaa ctcgcccaca agctcaagtt cgcgggctgg gacttcgtga tcatcgaagg
     4261 cgaagccaag agccccgtct acctctacat ccacaacgaa aaggtcgaga tccgcgacgc
     4321 ctccgccgcc tgggggcagg gcacccgccg ttccgccgag gtcctcgcca aggaaacgac
     4381 acccgaagcc agcttcggcg tgatcggcca ggccggtgaa aacctcgtgc cgatgtcgaa
     4441 catgatggtc gacaagagcc actccgccgg cggtctcggc tccgtgatgg gcaagaagca
     4501 cctcaagggc atcgtcgtct acggcaacca ggccatccac atccacgccg atccgaagga
     4561 atgggaggag ctcatcgact cccaccgcaa gatcctcggc gcgcacacgc actccatcgt
     4621 ctcgcgcaag ccgcatccga tgttcgagta ccacacgcct gcgagccgct ggtccgcctt
     4681 cccgggcgcc aagtggggtg ccgccaatcc gcccgtcatg ctcaccgacg cggacatgga
     4741 cccgcacaac ctcaaccaca tcggctaccg cacctgctcg tccgagttct acatgggccc
     4801 gacgatgtgg aactacacgg tgcgcaacac gggctgctac gcctgcccga tccgctgcta
     4861 ctccgtgatc cgcgacgatg aaacggccgc gaagtacaac gtcaacgcca tcaccgaaca
     4921 gacctgcatg gcgctctacg gccgctggtt cttcccgtcg ctcctcaagg acctgatgaa
     4981 gcccgtctcc cgcgaggcct gcctcgtcgg cagccagacg atggacgatc tgggcctatg
     5041 gtgcaactat ggccagctcc accgcgactt ctgcacgatg tacaccaagg gctacttcaa
     5101 gaagtacctg cccgaagagg agtacaagag catcgactgg tccaagatcg acaatcccga
     5161 tccgcgcatc ctccaggaca tcctgccgcg catcgcctac cgcaagggcg agttcggccg
     5221 ctggatgggc gagacgacgc ccgtcatgct cgagcacttc ggcctcaccg aagacgaatg
     5281 gaagaagaac cacgacacgc tctactggtc cgtcggccat ccgaagcacc acggcaatga
     5341 aaacgacggc cagatcggca cggttctcaa ctgcatgtac aaccgcgatc cgatgagcca
     5401 cggccacatc aacttctcga cctccggtct gccgctggag ctccagcgcg agatcgccgc
     5461 gaagttctgg ggcgacggct ccgccgtcga cggcatcggc gactatcgtc cgaccaacaa
     5521 gtacaagatg atccgcctgc gctgggtcat cgcccgcaag gagctgcacg acatgctcgg
     5581 catctgctcc tggacggccc cgtgggaact ctctccgctt cgcgagcgcg gctacatcgg
     5641 tgacatcgag atggaaagca aggtcttcaa ggccgtgacc ggcatctcca tgacccagga
     5701 cgagctcgac aaggccggcc tgcgcgcctt cctgctgcag cgcctctaca cgatgcgcca
     5761 gctcaagacg aaggacatgc gccacgtcca cgaccgttat cccgactgga tcttcgacga
     5821 cgccaagggt cgcgccccgt tcacgaaggg gacgatccgc atggagcacg acgacatcga
     5881 gaagagcttc gacctcttct tcgagctcat ggacttcgac gtgaagaccg gcgctccgac
     5941 ggaaaagtgc ctcaatgagt acggtctcgc caaggccgtt ccggtcatga agaaggaagg
     6001 tcttctctaa gtcatgggca aggaacggat gaagatgcaa gagaacatga atccggcgcc
     6061 cgaaggcgcc cagaccccgg cggacgaaga agctgcccgc gaggccgcaa ggctctcgca
     6121 gctcaaggcc gacatgctcg tcctcatcga cacggtggcc gacagcagcc gcgacgtgaa
     6181 gctcatcgac ggcgacacgc tctttgagcg cttcgcaaag agggcgagcg tcgccgaagc
     6241 gcccgaggtc gacgagctcg tgcgcgaagc cgaagccgac atcgacgcgg tccttggccg
     6301 cactgacggc gtctccgcca acgtggtgaa gttccgcagc aagagcgaga tgaaggcgca
     6361 tgccgacgag gttgcgaccc agaaggcgca gcgccgggtc ttctcgcgcc gcgaggtgat
     6421 ccagaacctc ctcgcgggca acgttgcgag cgtcgtgaag gccgccgagg cggccgacgc
     6481 gaagacggag cccgagggcg aggcgcgcga gatcacgcgc gagtacttcg acgaggtgct
     6541 ctcccgcgtg ctcgagggcg actacggcgt ctgttctctg gaaagctggg acaaggtgac
     6601 ctattaccac taccgtccgc tgctttcctg ctcgtacgcg cgcatcctct cggccgagaa
     6661 caatccggtc gagcaggtgg tcgacatgat ccgcgagtcg agccgcgtct atccgcgtcc
     6721 ggtggccatc ggcgtctttg aggagcctcc cttcggcatc gaggccgagg agctccagga
     6781 gatgctcaag atgatcgccc aggacccaga aaagcaggac attcgattta caacgagctc
     6841 cataggaacc gtcttcctgt actcgaacaa gtacctcgag gacggctacg ccgaattcct
     6901 tgccgaagag caggacgtcg gcgccgtgat gaatccgtag cccgttgtaa aaccaggaag
     6961 aattgaaatg aaattccaga tgaaatccac ggcgaaggcc gtcgcgctcg gcatcgtcac
     7021 cctggccgcc tccgcggcct tcgcaacgca gactcccgtc gagttcgacc tcaagggcca
     7081 cgacgagttc gtcaagtccc tcggcaagga cgcgatcgcg ccccagcaga ggactgccga
     7141 agaatccaag gccattctcg ccaaggtcta caacgagaag ctcgagcagc acgtctccag
     7201 cttccactat gaaaagggcg tcacctgcat ctcctgccac gaccagcaga agcagggcgg
     7261 cccggactgg atggttcccg tcaccaatcc tccgatgaag aaggaatgcg gcgactgcca
     7321 tgaaacccag gcctacgtgg tgagccacac caagtctcac agcaagtacg agtgcacggt
     7381 ctgccacatg ccgaacacgg ccgccggccc caacgacggc agcaacggca agatggacgc
     7441 cgtgcgccgc ctgcacacgt acaagatcaa cgtgagcccc gacgcctcca cctggacgaa
     7501 ggacaaggac ggcgcctggg tcctctcgaa ggacgaggac ggtcacggct acgtcgacct
     7561 gatgtggagc tgcgcgcgca atgcgcccgc cgactacacg gtcgccgagg gccgcggttg
     7621 ccactccaag gccacgagcg agcttgatcc cggcctcgtc tatcaggacg agaaggaggt
     7681 ctacggcgaa gtcatgaagt ggcagaagcc cgtgaaggac ggctacgagc agatcaaggg
     7741 cggcgtcgag cgcgtctcga agctccttga agtgacggcg ctgccgtacg agaagcagac
     7801 gcagatccgc ctgcttctcg acaaggccac ggaagtctcc gacctgatcg aaaaggacgg
     7861 cagctggggc gctcatgcct cccactacct ccaggaccgc gtgaagtcgg ccctgagcta
     7921 catcgaccag gcacagacgc tccttgacaa gggcggctac tgagaggcgc ctgaagcgtc
     7981 ctctcggtga ggcgggcgcc gccgcgaatg cggccgtcgc atgacggtcc gcggcggcct
     8041 cccgtccatt caggagaata atcgtgacga ctaaaatcac ccgacgcgcc ctgctgggcg
     8101 cagccggtgc gggcgtcgtg acccttggtt cggccgaagc ggccgccctt gcggcctctt
     8161 cggaggcttc gggcagccgc gagccgcact atgcgatggt cttcgaccag aacaagtgcg
     8221 tcggctgcgg cgactgcaag gccgcctgca acgaggccaa ccatctgcct cccggcatct
     8281 cgcgccttct catggagcgc cagtcgaagt cgcaccgcgt catcggcaag gagccgtgcc
     8341 cgatctgcgg caagctcgag gaatgcgact gcgagcgcaa gtacgtccgc gtctcgtgcc
     8401 agcagtgtca ggacgcgccc tgcgtgaccg tctgcccgac gggcgcggcc catcacgacc
     8461 cggaaaccgg catcgtgacc atggaccccg accgctgcgt gggctgcaag tactgcatcg
     8521 tggcgtgtcc ctacaacgcg cgcttcatca acgcggagac gaaggtcgcg gacaactgcg
     8581 acttctgcct ccacacgagg ttcgcggagg gccgcaagcc cgcctgcgtg gagagctgca
     8641 agtacgatgc gctcgttttc ggcaacgtga acgatccgta ctcgtacatc agccgcctca
     8701 tccgcgccaa ggacacggtc cgcatccgcc ccgagctcgg cacccgtccg agcctgaggt
     8761 acattccgtt cgtgaagaag ggggcgtgac atggacatga actttgcaat cggcctctcc
     8821 caaggcattg cctggccctg gcccatcgcc gtgtacctgt tccttgcagg catctccggc
     8881 ggcgcggtcg cggctgcggt catcctcaac ctctacagga agcaggcgca tgagaacacg
     8941 cccgtcctga aggcggcgtc ggtcatcggc gcggcgacca tcctgctcgg catggtctgc
     9001 ctcgtcctcg acttgacgaa tccgttcaac ttctggcgca ttctgatcta ctacaacccc
     9061 acgtccgtga tgagtctcgg cgtgatggcg ctgctcgtct acatcccgac ggtctgcatc
     9121 ctcacgctcg tggcgcttcg cggccttctc ccgaaggtcg gccttgggtt cctcgacggc
     9181 atcgtcgggt tcttcgacgg ctggcgcgtg gtgctgcagt ggatcgcgct cgtcttcgcg
     9241 ctcacgatct gcgcgtacac gggcttcctg atctcggcgc tcatccgctt cccgctgatc
     9301 aaccaggcgg tgcttcccgc gctcttcgtg gcgtcgggtc tctccgccgg catggccgca
     9361 acgaaggctt tcgcctgcct cgccttcggc gacaggcacg agagctgcga ccagcacctc
     9421 cttcacgcgg ccgaatggcc cgtgatggcg gccgaggcgc tctgcatctt catgatcgca
     9481 gtggccctcg tcaacgggaa cgccgccgcg caggccgcct ttgcggcctt cacgacgggc
     9541 gcctgggcga tcgagttctg ggtcggagtc gtgtgcgtcg gcttctgcgg accgctcgtc
     9601 ctgggcctga tcggcggcgg cctcatgtcg ttttacctga gcgctctgtg ctcggtggcc
     9661 ggcatgatgt gcctgcgtct cttcatcctc tatgcgggcc agctccacgg tctctgatga
     9721 cccggacccc gtgaactcaa gccgggcatc cgctcccttt cgggacggat gcccgcattc
     9781 tgcggagacg caacatgccc tgtcttccga acgatttccc cggcgcggtc tcgaccgtgc
     9841 ccgtcctcaa gaacttcatg agcctcgaga ccgaggacgt ggtcgccgaa gaggtgcccg
     9901 tcgcgctcgt ctacaacggc atctcccaca ccgtcatgat ggcctctccc gtcatgctcg
     9961 aggagttcgg caccggcttc agcctcaccg aaggcatcgt cgagtccgtc tcggacatct
    10021 acgacgtcga ggtgattgaa gcctgcggct cgggccgcga ggtgaggatc accatcagct
    10081 ccgaagcctt ccacaacttc aaggcccgcc gccgcgccat gctcgggcgc acgggttgcg
    10141 gcatctgcgg cgtcgagagc ctcggcgatg cgcttcgccc cgtgcccgat ctgcccgcca
    10201 cgcagacctt cgacatgagg ttctaccgcg acgccgtcgc gcagttgaag cgcttcgagc
    10261 gcatcggcgc gctcacgggc tgcacccatg cctccgtctg gatcagaccc gacggcacgc
    10321 ccgcgggcgg cgccgaggac gttggccgcc acgtggcgct cgacaagctc ctcggccttc
    10381 gcgcgcgcga gggctggcgg gacggcgcgc tcgtcgtgag ctcccgcgcc tcctacgaga
    10441 tggtccagaa ggccgccatg tgcggcgtcg agatcctctt tgccgtttcg gcccccacgg
    10501 cgctcgccgt gagaacggcc gaacgctgcg ggatgacgct cgcggccttt gcgcgtgcgg
    10561 gcggccccaa cgtctacacg catcccgaac gcctcgtcgg ggcgcaggac gaaaggctct
    10621 gcgcttaagt cggtcaagct caggcaagtg ttgggaaacg gggccttcgg ggcccgtttt
    10681 tcattctggg gatggggccg gatgttctgg gacaggaggg aaaagcgcag caacaataaa
    10741 atttataaag tcgagccgag aaaacccgcg gttttaaccg ccgggatgat aggcgctcaa
    10801 atccccttcg cacaaactcg tgcgaagcat gcgtcctcga aaaggacggt gtaggattga
    10861 ggtcacgaaa gccaacatcg cctacaagtt cgaactgatg cctacccggg agcagaagcg
    10921 aatcttcgct caagctgccg ggtgtcggcg tttcgtgtgg aacaaggcga tagaactgca
    10981 gaagcggtgc cttgaacaag aggttccttt tctcaagtac acggagctgg ccggacggtt
    11041 ggtgctgtgg aaaaaggaag acgacatgtt gtggttgcag gaagcgcctt ctcaggcact
    11101 tcaacagtgc cgttttcccg tgacggggag gcgacttgat gcagacgaca aaagaaacgg
    11161 cggactcccg agtgggagcc cgccgttgtt actggtgggt cccgcctcct tgcgagagga
    11221 gacgggggag agaggcttta gaacgtgtag aggacgttca cgctgccggt gccgccgcgg
    11281 cggtcgccgg catagccctt gaggcccacg ccgatggtcc agggaccgtt ggcttcgggt
    11341 ttcatggtga agccgaggtc gacgatggcg ctgtcgccct tgaggctcgg cgcatcaagc
    11401 gcggcggtgg cgcccgcgaa cttcagctcg ccctcggcct tgccgtcgaa gacgtgctcg
    11461 tatgcgaggc cggcgtacca gtcgaaggtc tggaaggcgc cttcaagacg agcgccgagg
    11521 cggaccgcat gggtc
//
LOCUS       DBMDTI010000041        11528 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1675_length_11528_cov_4.415770, whole genome shotgun sequence.
ACCESSION   DBMDTI010000041 DBMDTI010000000
VERSION     DBMDTI010000041.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 11528)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11528)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11528
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1675_length_11528_cov_4.415770"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="ACZPB6_03525"
     CDS             <1..597
                     /locus_tag="ACZPB6_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HIR1183577.1"
                     /translation="AFNASLFLSANPENVRNAIAAGQPAGLVMPSPVGDEDDDASPTL
                     RIAFDFDGVIADDESEREYQKEGLSGFHRLEQEKASTPHSPGPLRRLFEQLSAFQRFD
                     YNHRGAADPYFEPAVRISIVTARGAPSEERLITTLKSFGMSAAELFLLDGLDKQPFLK
                     AIRPHIFFDDQANNLKPSLNIVPSVLVPFGIHHLEKLP"
     gene            602..2632
                     /locus_tag="ACZPB6_03530"
     CDS             602..2632
                     /locus_tag="ACZPB6_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1183578.1"
                     /translation="MAAYPHLFLPLKVGSHFLKNRIVMAPVETGLEKQDYFDPRVVEF
                     YEERARGDGPGLFILGNGVVHETGLRNLKDPVLTAGLLNNAVRLTSVIHESGSKVLLQ
                     LQHHGAGADHMFAVSASRFRNRDTGRTLHRAPGLLVSHLISQYSLFAYHAILHGEFDG
                     VEIYGGRLSLPNVFSSRLFNRRHDKWGLQARTLFAVELVRRIRSFVGPAPIISYRLSL
                     LDLHAGGSEWHDLLAFAQALHYEGVNLFSFDIGFTPNSVPVNSDLTPAGVWVPFMEKF
                     SREIKVPVIFGHRMPGPDRLDDILQNNITSLVEIGRPLIADARWVEHVHTGKPVRPCT
                     LCPHGCTRPDRREGREMLACIADPYVLRRSHKHPGGLDILVVGGGPAGMAAAREGALR
                     GHKITLVDEGPELGGLYRLCAKIQGRSSIAELLKVQERELVELGVEIIRNTRATAQWI
                     EDNYSGSRILLATGKESCMPDIPGIDTPNVLSLEDLLQGRVPVGHRVAVIGSGNVAVD
                     VTRHLCTHDLKAHDEWCCAWGIGDPAEHIGGTLGVIPHLNPAPRKVYLINETNETTEE
                     LFAHERRLYEVQWLRMHGVNIFDEANVEQIDSHSVRVSCEGEEGSTILRVDHIVVVGD
                     REANAELQNELTELGIPFEAAGSMKFKAPFGTAGEAALDGIYVLQALEKRSL"
     gene            3126..3917
                     /locus_tag="ACZPB6_03535"
     CDS             3126..3917
                     /locus_tag="ACZPB6_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1183579.1"
                     /translation="MAPEVIPWMSPCLSQSMSFVLAGIGHPMKFRRGDVIYTSPGLFG
                     NLMYVRSGFVVKALLDPVRDEPLLLSMAGPGALCGNYENLYVRDRMPRRHWCLTSTEV
                     LVVNQELLLKIADQNPEWQHELSGYSSVSSICDRMGMFLNYSGTVEERLGALLLLMLH
                     CEKPAAMANLFSKGVEWVELPFYPDKGTIARLLGCKFETVSDVVRQWGSHDDIRRRSR
                     RIWLRRRTFLKYWEWLMPFMQQSRAEERAAEALRQARRREEYDSF"
     gene            3904..4548
                     /locus_tag="ACZPB6_03540"
     CDS             3904..4548
                     /locus_tag="ACZPB6_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR1183580.1"
                     /translation="MTVSKVFVLAAAVATVGLSGCAPILIGGAAVTSATVITDRRTTG
                     SIVDDEVLEKRVRYEIGEVIGHEKHHVVVTSYEGKVLLTGEVLTAADRQKAQDTAVKS
                     VGVHSVINELAVMTPATVGAVLSDSMLATKVRSSIIGTKKISLNQMKVDVQRGIVYLM
                     GIVTIDEARTATKVAAGVSGVQQVVTCFTIESEAAIKERLKNLESNNSSKSAEP"
     gene            complement(4625..5536)
                     /gene="menA"
                     /locus_tag="ACZPB6_03545"
     CDS             complement(4625..5536)
                     /gene="menA"
                     /locus_tag="ACZPB6_03545"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HIR1183581.1"
                     /translation="MTGVEDVKPGSLKAWMAFTRPKTWGVAVAPVIAALSLALFETGR
                     FDALTAFFTMTIALLMQIISNMKNDLGYTEKKAETGNRRGLPRATTQGWISISAARRA
                     ILTLIVLALLNTAVLIWLGGWVFALIGISSVIAAYSYMGGPKPIAYTPFGEATVLVFF
                     GLTAVCGSYYLQTFTVSANAVLLSISLGSIAAAVLAVNNWRDRVHDKSIGRQTLAVVL
                     GDKTFTAVFRIMTALPLALGLVMAAAPGFWPCLLVLLCLPLCLPLWKQFGTLQHEALN
                     ATMFGCVKYELAYSVLFSLGALLACLL"
     gene            complement(5536..5829)
                     /locus_tag="ACZPB6_03550"
     CDS             complement(5536..5829)
                     /locus_tag="ACZPB6_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HIR1183582.1"
                     /translation="MNDNSKPQPDDLTPVMVFPMLNFPIKIMGLNQPEFPVAISELAS
                     GHFADFDKGTMTTALSKTGKYLALSIMVNAQSQEQLDNFYRALTAHPMVKVAL"
     gene            complement(5826..6677)
                     /locus_tag="ACZPB6_03555"
     CDS             complement(5826..6677)
                     /locus_tag="ACZPB6_03555"
                     /inference="COORDINATES: protein motif:HMM:NF019555.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183583.1"
                     /translation="MRFSTSPLLSAVMLCAASQSAVGAMALMDLDSRQILQLEGNLLF
                     ITEPLAEQLKGFPGMADKPVKAGETATEAFVARLAAISDEQWKAFLSKTAADDGQEAA
                     RRLAPVAAACTDIGCDGIYVSVNPLGNGETHRLLAVVFGSDTRETLDRAAGVFLREGH
                     LRYKTATVIRNGEAVGRTPVFKGEAQDVTLVTHENVVVTIAKTMTLSKDSEAFKMHLT
                     RQQPVIAPVDAETPLGTLSIWLNGKCLRSVPVYAKEPVKEGSLWQRVRDTIILTTTDI
                     NEAEKSR"
     gene            6795..7556
                     /locus_tag="ACZPB6_03560"
     CDS             6795..7556
                     /locus_tag="ACZPB6_03560"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR1183584.1"
                     /translation="MLPTKEDKSVESFDVRVVEETGSTNDDMKTYARSSRVSRPLVLE
                     AKHQTMGRGTRGRGWKSPEGCLAFSLLLPFSMIRVPVSLVAIAVGMGVCSALRDAGRF
                     ACIKWPNDVWFEGGKCGGILCESVKDADGRSVLIAGVGLNRDGAAERTTNGWPISGLG
                     DVLACSDHAAPNPMLGPLVTGILGILTRDRADVAAAWPFYDAFAGRTIRLEDDGGAWK
                     GLNMGIDSLGRLQLKTNEGVKAFFSGTFFNDDAAY"
     gene            7537..8331
                     /locus_tag="ACZPB6_03565"
     CDS             7537..8331
                     /locus_tag="ACZPB6_03565"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR1183585.1"
                     /translation="MTTLLIDLGNTALKWTPIEEPDNPHTIIHKDSTHFKEELYDLWL
                     NMRPKRIIGCTVSAPVVAFSLTKFFNDHGIRWNWVRTQPSFHGRMTLNNCYENPGQLG
                     ADRWYAAVGAADAMLEERRSLLVVHAGTATTVDSILYRESGVYDFMGGRITLGPTLMK
                     TFLTKGIPSLTDLDGAYDALPRSTRDAVMTGIIDAQVGLILRARSVMRRQGFDPKVLL
                     AGGSAKFIAPYLQEEVPDLIVKHNLVLRGLSALAGQQAEGLGREDA"
     gene            8328..9065
                     /locus_tag="ACZPB6_03570"
     CDS             8328..9065
                     /locus_tag="ACZPB6_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183586.1"
                     /translation="MRYFAVTLLLAAMGAAGYLAWTGLHQTPEVIPSGEVKTVRVDNF
                     AYASPELVKPENLKVEAPAVRDEAVQAAEAAVEEAAPVLEPVDEAKLEGLTCVVFGPL
                     QEKRLPKHRRFFEKVDLIRKMNIVPYSDTRYAVYAGPFTQQKARKNRKALCGDDLESC
                     SIMELKAGGHAVVLKTFSDEKAAELWSKKFALDNGLTNVRVTRYNHVAGSRVVLVFNG
                     IADQQVKALLKRSKAEGIGLSVCPKGL"
     gene            complement(9066..9497)
                     /locus_tag="ACZPB6_03575"
     CDS             complement(9066..9497)
                     /locus_tag="ACZPB6_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR1183587.1"
                     /translation="MTCPLCTDRAKSEIWRNGNFYLIDAEDPAFPCFLRVVSCRHVPE
                     MSSLTSEERTELWGILNVVEEEIIKALRPHKVNLAQFGNMVPHLHWHVIARWQDDSHF
                     PECPWGPVQRDVPADVQAERRRMTEALKPVLREALEKRFGC"
     gene            complement(9509..9724)
                     /locus_tag="ACZPB6_03580"
     CDS             complement(9509..9724)
                     /locus_tag="ACZPB6_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183588.1"
                     /translation="MRRDCVTQIIVDWGNGEWENFATPFEAEQYINAMLDELDVPKAA
                     WREDMQGNKKWDYEIVEDDNGLIRLVD"
     gene            complement(9727..11139)
                     /locus_tag="ACZPB6_03585"
     CDS             complement(9727..11139)
                     /locus_tag="ACZPB6_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1183589.1"
                     /translation="MSLPSNVYESIHRAWMGDTLEGLKDFIRIPAKSRAFNPNWESDG
                     ILKRTLEDSAEWGRRLLPCAEFEIIEKPGIPPALFIRIPTAGGHKGKPAFFYGHLDKQ
                     PETEGWSEGLGPWTPVVRDGRLYGRGGVDDGYNYYLTLTAVKALEEAGAAHPEIFCLF
                     ETDEESGSRDLARYIQELAPRIGDPAFIAIADLGCNDMKRVWLTQSLRGTVSLTVDVR
                     VLNTPSHSGLASGVVPSSFRIMRALLDRIEDAATGELLLPELNCEVPPDILECLRSLA
                     QEGDVRECFDWAGDTHAQAETAFEALLTNTWKPSMCVVGADGLPPCDNAGNVLRSNTS
                     LRLSFRTPPLVDAKAALEAVIRTLTENVPCGAQVTVSRAEAASGFVSPLPSGWLRETL
                     EDAGASIFGNSTGYLFCGASIGTLPAFKAAFPTSPFVNTGALGPTCNAHAPNEWLDLA
                     YAEKLTCVFAELIAGIPSEN"
     gene            complement(11176..>11528)
                     /locus_tag="ACZPB6_03590"
     CDS             complement(11176..>11528)
                     /locus_tag="ACZPB6_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR1183590.1"
                     /translation="GTWQSGNGLTDPVTNSTQVQAPVQSATPSADAVVTAPAAGWSVQ
                     IGFFTERDNALAYAAHAEAVLASTSSARSARLVRDNGGYRVVLGNGMSLNEARAAALN
                     VKDIIGSGAFAVQK"
BASE COUNT         2203 a         3423 c         3688 g         2214 t
ORIGIN      
        1 gccttcaacg cctctctctt tctgagcgcc aaccccgaga atgtccgcaa tgccatcgcc
       61 gcaggccagc ctgcgggcct cgtgatgccg tctcccgtcg gagacgagga tgacgacgcc
      121 agtccgacgc tgcgcatcgc cttcgacttc gacggcgtga tcgccgacga cgaaagcgag
      181 cgcgagtacc agaaggaggg gctctcgggc tttcatcgac tcgagcagga aaaggcctcc
      241 acgccccatt cgccggggcc cctgcgcagg ctcttcgagc agctgtcggc ctttcagcgc
      301 ttcgactaca accacagggg cgccgcagac ccgtacttcg agcccgccgt ccgcatctcc
      361 atcgtgacgg ctcgaggcgc gcccagcgag gagcgcctga tcaccacact gaaaagcttc
      421 ggcatgagcg ccgcggagct ctttctgctc gacggtctcg acaagcagcc gtttctcaag
      481 gccatcaggc cgcacatctt cttcgacgac caggcgaaca acctgaagcc gagcctcaac
      541 atcgtgccgt ccgtgctggt gcccttcggc atccatcatc tggagaagct tccctagcat
      601 catggccgcc tatccgcatc tgtttctgcc tctcaaggtg ggatcgcact tcctgaagaa
      661 caggatcgtc atggcccccg ttgagacagg cctcgaaaag caggactact tcgacccgcg
      721 cgtcgtggag ttctacgagg aacgcgcgcg tggcgacggg ccggggctct tcatcctcgg
      781 caacggcgtc gtgcacgaaa ctggtctgcg gaatctcaag gaccccgtcc tgacggccgg
      841 cctgctcaac aatgcggttc gcctgacatc cgtcatccat gagtcgggct ccaaggtcct
      901 tcttcagctt cagcaccacg gcgccggggc agaccacatg tttgccgtct ccgcctcgcg
      961 ctttcgcaac agggacacgg ggcgcacgct gcacagggct ccggggctcc tggtgagcca
     1021 cttgatcagc cagtactccc tctttgccta ccacgccatc ctgcacggag aattcgacgg
     1081 cgtggagatc tacggcggcc gcctttccct gccgaacgtc ttttcgagtc gcctcttcaa
     1141 ccgccgccac gacaagtggg gcctgcaggc ccgcacgctc tttgcggtcg agctcgtccg
     1201 ccgcatccga agcttcgtcg gaccggcgcc gatcatctca taccgccttt cgcttcttga
     1261 cctgcacgcg ggcggcagcg aatggcacga cctccttgcc tttgcccagg cccttcatta
     1321 cgaaggcgtc aatctcttca gtttcgacat cggctttacg cccaacagcg ttcccgtcaa
     1381 cagcgacctg acgcccgcag gcgtctgggt gccgttcatg gagaagttct cccgggagat
     1441 caaggttccc gtcatcttcg gccaccgcat gccggggccc gacaggcttg acgacatcct
     1501 gcagaacaac atcacgagtc tcgtcgagat cggtcgtccg ctcattgcgg acgcacgctg
     1561 ggtggagcac gtgcacacgg gcaagcccgt caggccatgc acactctgtc cgcacggctg
     1621 cacgcgcccc gacaggcgcg agggcaggga aatgctggcc tgcattgcag acccatacgt
     1681 cctcagacgc tctcacaagc atcctggcgg cctcgacata ctcgtcgtag ggggaggccc
     1741 tgccggcatg gctgcggccc gtgagggcgc tctgagaggc cacaagatca ccctcgttga
     1801 cgaaggtccc gaacttggcg gcctttacag gctctgcgca aagattcagg gccgaagctc
     1861 aatcgcggag cttctcaagg tgcaggagcg cgagctcgtc gagctcggcg tcgaaatcat
     1921 ccgcaacact cgcgccaccg cgcagtggat tgaggacaac tacagcggaa gcaggatcct
     1981 gctcgccacg ggcaaggaat cctgcatgcc cgacattccc ggcatcgaca cgcccaacgt
     2041 cctttcgctt gaggacctgc tgcagggccg cgtgcccgtc ggccaccgtg tcgccgtcat
     2101 cggcagcgga aacgtcgccg tggacgtcac gcgccatctc tgcacccacg acctgaaggc
     2161 gcacgatgaa tggtgctgcg cctggggcat cggcgacccc gccgaacaca tcggcggcac
     2221 gctcggcgtc atcccccatc tcaaccccgc tccgcgcaag gtctacctca tcaatgaaac
     2281 gaatgagacc accgaggagc tctttgcaca cgagcgcagg ctctacgaag tccagtggct
     2341 gcgcatgcac ggcgtgaaca tcttcgacga ggccaacgtc gaacagatcg acagccactc
     2401 ggtgcgagtg agctgcgagg gcgaagaagg ctccaccatc cttcgcgtcg accacatcgt
     2461 cgtggtcgga gaccgcgagg cgaatgcaga actccagaac gagctcaccg agctcggcat
     2521 tccctttgaa gcggcgggca gcatgaagtt caaggccccc ttcggaacgg cgggagaggc
     2581 tgctctggac ggcatttacg tccttcaggc actcgaaaag cgcagtctct gagttttcgc
     2641 ctccaaaaca aaaacttttg gggcacagta ataaatgcac ccaatccaca gggaatacac
     2701 aggataaccc acaagttatc cacaggttgg cgatcggaaa tgggcgcatt ttaaagccct
     2761 cttttgatct ccagtcgcct tctccccggt tcttacgttt ctacattttc cgttttttgt
     2821 gtaaagttga aggatcctcg caaagttcta ctctatatat ttcttcttaa attctttatg
     2881 ttagtagtgg taagtagtag gtgaggacgg tttttgtgga taatagactt ttgtacctaa
     2941 aaaacaataa gatgcacctg tgaaaagcgt gtggacaagc ctctggatct cctgtgggaa
     3001 agacgggaaa agcaggggac aactttgggc tccggaaggt gcacaaaaaa gttatccaca
     3061 aaatcgagaa tcttcatcag aagttaccca caaagttgac cacaaatttt taaacatttt
     3121 tgtttatggc ccccgaagtc attccctgga tgtccccctg cctttcgcag tcgatgagct
     3181 ttgtgttggc aggcatcggg catcccatga aattccgtcg cggcgacgtg atctacacct
     3241 ctcccggtct gttcggcaat ctgatgtacg tgcgttcagg atttgtggtg aaggctctgc
     3301 tggatccagt gagggacgag cccctgcttc tgtcgatggc ggggcccggc gcgctgtgcg
     3361 gcaattatga gaatctgtac gtgagggacc gcatgcccag acgtcactgg tgtctgacgt
     3421 cgaccgaggt gctggtggtg aaccaggagc tgcttctgaa gatcgccgac cagaatcccg
     3481 agtggcagca cgagctgtcg ggatactcct ccgtctcgtc gatctgcgac aggatgggta
     3541 tgtttctcaa ttattccggc acggtcgagg agcgcctcgg cgcattatta ttgttgatgc
     3601 ttcactgcga gaagccggcg gccatggcga atcttttctc caaaggcgtg gaatgggtgg
     3661 agctgccctt ctatcccgac aaggggacga ttgcaaggct gctcggctgc aagttcgaga
     3721 ccgtcagcga cgtggtcagg cagtggggct cgcatgatga catccgcagg cgaagccgga
     3781 ggatctggct gaggcgccgg acgtttttga agtattggga atggctcatg ccgttcatgc
     3841 agcagagccg cgccgaggag cgtgcggcgg aggccttgag acaggcccgg aggagggaag
     3901 aatatgacag tttctaaggt gttcgttctg gctgccgccg tcgcgacggt cggcttgagc
     3961 ggatgtgcgc cgattctgat cggcggcgcg gccgtcacgt cggcaaccgt cattacggac
     4021 cgtcgaacga ccggcagcat tgtcgacgac gaagtgctcg agaagcgcgt tcgctatgaa
     4081 atcggcgagg tcatcggaca tgaaaagcac cacgtggtcg tgacgagcta cgagggcaag
     4141 gtgcttctga cgggcgaagt gctcacggcc gccgacaggc agaaggccca ggacacggcc
     4201 gtcaagagcg tgggcgtgca ttccgtgatc aacgaactcg ccgtgatgac gcccgccacc
     4261 gtgggtgccg tcctttccga ctcgatgctc gcgaccaagg tccgcagttc gatcatcggc
     4321 accaagaaga tttcgctcaa ccagatgaag gtcgacgtcc agcgcggcat cgtctatctg
     4381 atgggcatcg tgacgatcga tgaggcaagg accgccacca aggtggccgc cggagtttcc
     4441 ggcgttcagc aggtcgtgac ctgcttcacg atcgaaagcg aagcagccat caaggagcgt
     4501 ctcaagaatc tcgagtcgaa caacagctcg aagagcgccg aaccgtaagc cccgtgccct
     4561 gaaggataaa aaactctccg cccgcctgca gcgggggaga gttttttgtt gatgattcgg
     4621 cgcctcagag aaggcaggca agcagtgcgc ccagagaaaa aagcaccgaa taggcgagtt
     4681 cgtacttgac gcatccgaac atcgtggcgt tgagcgcctc gtgctgcagc gtgccgaact
     4741 gcttccacag cggaaggcac agcggcaggc agagcagtac aagcaggcag ggccagaatc
     4801 cgggcgcggc ggccatgacg aggccgaggg caagcggcag cgccgtcatg attctgaaga
     4861 ctgccgtgaa ggtcttgtcg ccgagcacga ccgcaagcgt ttggcggccg atgctcttgt
     4921 cgtgcactct gtccctccag ttgttgacgg cgagcacggc ggccgcgatc gagccgagcg
     4981 agatgctcag gaggacggcg tttgccgaca ccgtgaaggt ctgcaggtag taggagccgc
     5041 agacggccgt gagaccgaag aatacgagca ccgtcgcctc tccgaacggc gtgtaggcaa
     5101 tgggcttcgg accgcccatg tacgaatagg cggcaatgac cgaggagatg ccgatcagcg
     5161 cgaagaccca gccgccgagc cagatgagca cggcggtgtt gagcagtgcc agcacgatga
     5221 gcgtgagaat ggcccttcgg gcggcagaaa tggagatcca gccctgggtg gttgcacggg
     5281 gaagccccct gcggttgcct gtttccgcct tcttctccgt atagcccaga tcgttcttca
     5341 tgttgctgat gatctgcatg agcagagcga tcgtcatcgt gaagaacgcg gtcagggcat
     5401 caaatctgcc cgtttcgaac agggcgaggc ttagcgcggc aatgacggga gccacggcca
     5461 cgccccatgt cttcgggcgc gtgaaggcca tccaggcctt caggctgccg ggcttgacgt
     5521 cctcgacgcc cgtcatcaga gcgccacctt gaccatcggg tgcgcggtca gggctcggta
     5581 gaagttgtcg agctgttcct gagactgcgc gttcaccatg atgctgagcg caagatactt
     5641 gccggtcttg gagagggccg tcgtcatcgt gcccttgtcg aagtcggcga agtgtccgga
     5701 ggcgagttcg ctgatcgcga caggaaactc aggctggttg aggcccatga tcttgatcgg
     5761 gaagttcagc atgggaaaga ccatgaccgg agtcaggtcg tcgggctggg gcttggaatt
     5821 gtcgttcatc gggatttctc cgcttcgttg atgtctgtcg ttgtcagtat gatcgtgtcc
     5881 cggacccttt gccacaggga gccttccttg acgggctcct tcgcgtagac cggcacggac
     5941 cgcaggcact tgccgttgag ccagatgctc agcgtgccca gcggcgtttc ggcgtccaca
     6001 ggggcgatga cgggctgctg acgggtgagg tgcattttaa atgcttccga gtccttgctc
     6061 agcgtcatgg tcttggcaat cgtgacgacg acgttctcgt gcgtgacgag cgtcacgtcc
     6121 tgagcctctc ccttgaagac gggggtgcgt cccacggcct ctccgttgcg gatgaccgtt
     6181 gcggtcttgt agcgcaggtg gccctcacga agaaagacgc ctgccgccct gtcgagggtc
     6241 tcacgggtgt cggatccgaa gacgacggca aggagacggt gtgtctcccc gttgccgagc
     6301 ggattgacgg acacgtagat gccgtcgcag ccgatgtccg tgcaggccgc ggcgacggga
     6361 gcaagccgtc tggccgcctc ctgcccgtcg tcggcagcgg ttttcgaaag aaaggccttc
     6421 cactgctcgt cggagatcgc ggccagacgc gcaacaaagg cttccgttgc ggtttcacca
     6481 gccttgacgg gcttgtccgc cattcccgga aagcctttga gctgttccgc cagaggctcg
     6541 gtgatgaaga gcaggttgcc ttccagctga agaatctgtc tgctgtccag atccatcagg
     6601 gccatggcgc cgacggcaga ctgcgacgcg gcgcagagca tgacggcgga cagaagcggg
     6661 gatgttgaga agcgcattgg gagagaggat gattgggaac gcgttgccaa ctcattgtaa
     6721 tcaacgcctg agtatgcctg tcaaagcttt ctcaaagcct tcgggaattc aactatgatt
     6781 gacggacttt agaaatgctc ccgacgaagg aggataagtc cgtggagtcc tttgacgtgc
     6841 gcgtggttga ggagacaggc tcgaccaacg acgacatgaa gacgtatgcg cgcagctcgc
     6901 gcgtgagccg cccgctcgtg ctggaggcga agcatcagac gatggggcgg ggtacgaggg
     6961 gccgcggctg gaagtctcca gaaggctgcc tggccttttc tcttctgctg cctttttcca
     7021 tgatcagggt gcccgtgtcg ctagtggcca tcgccgtcgg catgggcgtg tgcagcgcgt
     7081 tgcgcgacgc aggccgcttc gcctgcatca agtggcccaa cgacgtctgg ttcgagggcg
     7141 gaaagtgcgg cggcattctc tgcgagtcgg tgaaggacgc cgacgggagg agcgtcctga
     7201 tcgcaggcgt gggcctcaac cgtgacggcg ccgccgaacg aacgacgaac ggctggccga
     7261 taagcggtct cggcgacgtg ctggcgtgct ccgatcacgc tgcgcccaat cccatgctcg
     7321 ggcctcttgt gacgggaatc ctcggcattc tgacccgcga cagggcggat gtggcggccg
     7381 cctggccctt ctacgacgcc tttgccggaa gaacgatccg tctcgaggac gacggtgggg
     7441 cgtggaaagg acttaatatg ggcattgaca gtctcggccg tctgcagctg aagacgaatg
     7501 agggcgtgaa ggcctttttt tctggaactt ttttcaatga cgacgctgct tattgacctc
     7561 gggaacaccg ctctgaaatg gacgcccatt gaggagcccg acaacccgca caccatcatc
     7621 cacaaggaca gcacgcattt caaggaagag ctctacgacc tgtggctcaa catgcgcccc
     7681 aagcgcatca tcggctgcac ggtctcggcg cccgtcgtcg ccttttcgct caccaagttc
     7741 ttcaatgacc acggcattcg atggaactgg gtgcgtacgc agccgtcctt tcacggtcgc
     7801 atgacgctca acaactgcta tgaaaacccg ggacagctcg gcgcagaccg ctggtatgcc
     7861 gccgttggcg cggccgacgc catgcttgag gaaaggcgct cgcttcttgt cgtgcacgca
     7921 ggcacggcca cgaccgtcga ttccatcctt taccgcgaga gcggcgtcta cgacttcatg
     7981 ggcgggcgca tcacgctcgg tcccacgctg atgaagacct tcctcacgaa gggcatcccc
     8041 tcgctcaccg atctcgacgg cgcctacgac gcgctgccgc gctccacgcg tgacgccgtc
     8101 atgacgggca tcatcgatgc gcaggtgggg ctcatcctgc gtgcgcgcag cgtcatgagg
     8161 agacagggct tcgatcccaa ggtgctgctt gccggcgggt ccgccaagtt catcgcaccc
     8221 tatctgcagg aggaggtgcc tgatctcatc gtcaagcaca atctggtgct gaggggactt
     8281 tccgctcttg cgggacagca ggctgaagga ctcgggaggg aggacgcatg agatattttg
     8341 ccgtcacgct tctgctggca gcgatgggtg ctgcgggcta tctggcctgg accggccttc
     8401 accagacccc cgaggtcatt ccgtcgggcg aggtcaagac cgttcgggtg gacaacttcg
     8461 cctatgcctc tcccgagctt gtgaagcccg aaaatctgaa ggtcgaggcg cctgccgtgc
     8521 gtgacgaggc cgttcaggcg gcggaggcgg cggtcgagga ggccgcgccc gttctggagc
     8581 ccgttgacga ggcgaagctc gaggggctga cgtgtgtcgt tttcggtccc ttgcaggaaa
     8641 agcgccttcc caagcaccgc aggttctttg aaaaagtcga cttgatcaga aagatgaaca
     8701 tcgttccgta cagcgatacg cgctacgccg tctatgcggg gcccttcacg cagcagaagg
     8761 cccggaagaa ccgcaaggcg ctttgcgggg acgatcttga gagctgctcg atcatggaac
     8821 tcaaggcggg cggccatgcc gtcgtgctga agaccttctc cgatgagaag gcggcggagc
     8881 tctggtcgaa gaagttcgcc cttgacaacg gtctgacgaa tgtcagggtg acccgctaca
     8941 accacgttgc gggcagccgc gtcgtgcttg tcttcaacgg gattgcggac cagcaggtca
     9001 aggcgctgct caagcgctcg aaggccgagg gaatcggcct gagcgtctgt ccgaagggat
     9061 tgtgatcagc agccgaagcg cttttcgagc gcttcgcgca gcacgggctt gagcgcctcc
     9121 gtcatgcggc ggcgctcggc ctgaacgtct gcgggaacgt cgcgctgcac cggcccccag
     9181 gggcattcgg ggaagtggct gtcgtcctgc cagcgcgcga tgacgtgcca gtgcaggtgc
     9241 ggcaccatgt tgccgaactg ggcgagattc accttgtgcg ggcggagcgc cttgatgatc
     9301 tcctcctcaa ccacgttgag aatgccccag agctccgttc tttcctcgga cgtgagcgag
     9361 gacatttcgg gtacatgtcg acatgagaca accctcagga agcaggggaa ggcgggatcc
     9421 tccgcatcga tgaggtagaa gtttccgttg cgccagattt cggacttggc gcggtcggtg
     9481 caaagcgggc aggtcatgtt gttctccttc agtccacgag cctgatgagg ccgttgtcgt
     9541 cctcgacgat ttcgtaatcc catttcttgt tgccctgcat gtcctcgcgc caggcggctt
     9601 tggggacgtc gagctcgtcg agcatggcgt tgatgtactg ctcggcctca aacggggtgg
     9661 cgaagttctc ccattcgccg ttgccccagt cgacgatgat ctgggtgacg cagtctcttc
     9721 tcatgatcag ttctccgacg gaatgccggc gatgagttcg gcgaagacgc aggtgagctt
     9781 ttcagcgtag gcaaggtcca gccattcgtt gggagcgtgt gcgttgcacg tggggccgag
     9841 tgcgcccgtg ttgacgaagg ggctcgtcgg gaaggcggcc ttgaaggcgg gcagcgtgcc
     9901 gatggaggcg ccgcagaaga ggtagcccgt gctgttgccg aagatcgagg cgcctgcgtc
     9961 ctcgagcgtc tcgcgcagcc agccggaagg cagcggcgaa acgaagccgg aggcggcttc
    10021 tgcgcggctc acggtcacct gtgcgccgca gggcacgttt tcggtcaggg tgcggatgac
    10081 ggcttcgagt gcggccttcg cgtcaacgag aggcggggtc ctgaaggaca ggcgaagcga
    10141 ggtgttggat cggagcacgt tgcccgcgtt gtcgcagggc gggagaccgt cggcgcccac
    10201 gacgcacatg gagggcttcc aagtattcgt cagcagggcc tcaaaggcgg tctcggcctg
    10261 agcgtgcgtg tcgcccgccc agtcgaagca ctcgcgaacg tcgccttcct gagcaagcga
    10321 gcgaaggcat tcgagaatgt cgggcggtac ttcgcagttg agctcgggga gcagaagctc
    10381 gcccgtggcg gcatcctcga tgcggtcgag cagggcgcgc atgatgcgga aggagctggg
    10441 cacgacgccg ctggccagac ccgaatgaga cggcgtgttg agcacgcgca cgtcgaccgt
    10501 gagcgagacg gtgcctcgca acgactgggt gagccagacg cgcttcatgt cgttgcagcc
    10561 gaggtcggcg atggcgatga aggcggggtc gccgatgcgc ggcgcaagtt cctgaatgta
    10621 ccgggccaga tcacgggagc cggattcctc gtccgtctcg aagaggcaga agatttcggg
    10681 atgggctgcg ccggcttctt ccagagcctt cacggcggtg agcgtcagat agtagttgta
    10741 gccgtcgtca acgcctccgc ggccgtagag gcggccgtcg cgcacgacgg gcgtccaggg
    10801 gccgaggccc tcgctccagc cttcggtttc aggctgcttg tcgagatggc cgtagaagaa
    10861 tgcaggcttg cccttgtggc cgcctgcggt gggaatgcgg atgaagagcg cgggcggaat
    10921 ccccggcttt tcaatgattt cgaattcggc gcagggcagg aggcggcggc cccattcggc
    10981 cgaatcctca agcgtacgct tcagaatgcc gtcggactcc cagttgggat tgaaggcgcg
    11041 ggacttggcg ggaatgcgga tgaagtcctt caggccttca agcgtgtcgc ccatccacgc
    11101 acggtggatg gattcgtaga cattggacgg gagagacatg aaatttcctc gacgaatttt
    11161 cgaaaacttg agcggttatt tctgaacggc aaaggcgccg ctgccgatga tgtccttgac
    11221 gttcagggcg gctgcgcgcg cttcgttgag cgacatgccg ttgccgagca cgacgcggta
    11281 tccgccgttg tcgcggacaa ggcgggcgct tcgggcggac gacgtggagg ccagcacggc
    11341 ctcggcgtgg gcggcgtagg caagcgcgtt gtcgcgctcc gtgaagaagc cgatctgcac
    11401 gctccagcct gcggcaggcg cggtcacaac ggcgtcggcg gacggcgtgg cggactgcac
    11461 gggcgcctga acctgcgtgg agttcgtgac gggatcagtg aggccgttgc ccgactgcca
    11521 cgtgcctg
//
LOCUS       DBMDTI010000042        11184 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1738_length_11184_cov_3.022106, whole genome shotgun sequence.
ACCESSION   DBMDTI010000042 DBMDTI010000000
VERSION     DBMDTI010000042.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11184)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1738_length_11184_cov_3.022106"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            105..1589
                     /gene="putP"
                     /locus_tag="ACZPB6_03595"
     CDS             105..1589
                     /gene="putP"
                     /locus_tag="ACZPB6_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HIR1183591.1"
                     /translation="MFSNPTAITFVGYLLVMVCVGFYAYYHTRSYGDYILGGRSLGGF
                     VTALSVGASDMSGWLLMGLPGAVYLAGISQSWIGIGLVLGSYINWCVVASRLRVYTEK
                     THNALTLPDYFSHRFEDKQNLLRIIAAVVILLFFTIYCASGVVAGARLFENTFSMSYE
                     TALILGAAATICYVFVGGFLAVSWTDTIQALLMCLALIVTPIAVMYDLGGFTAATECV
                     ASVDIKMLNILEGHTLVGIVSLLAWGLGYFGQPHILVRFMATRSIKVIPNARRVATLW
                     MSFCLSGAVAVGFFGAAFFAEHRDVASLVEQNHERVFIVLSGLLFNPWIAGILMSAIL
                     AAVMSTLSCQLLVCSSTLTEDFYRTFIRRNASQRELVWFGRSMVLLVSVVAVWLARDP
                     NSLVLSLVSYAWAGFGAAFGPVIIMSLWWRRMTRNGALAGMITGAVTVLVWNHYAWLG
                     LYEILPGFVFALIAIVVVSLLDKAPSKSIAKTFDEVQAAVRNPA"
     gene            1781..3037
                     /locus_tag="ACZPB6_03600"
     CDS             1781..3037
                     /locus_tag="ACZPB6_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183592.1"
                     /translation="MARSSHLAAQRRELSFILNRLTAYTTLADRDILEQRLRTILEKP
                     LSDDFYIMANEVFDKIEAQHPSVANEARGNFYGMLSEVKYEYRTPTESTSMLMVGLMM
                     STFFKTACPNVTLSQETADKVVEILKKHYINPKAKITIFHEMLRSNQGPANDTETAGR
                     LTRAMAECKGTLYTKEHLDVPEGEDDPIAALENERIYGLYLRHLVITVTVPAGELAVV
                     HPYRWWDTTRHPEFCVSGHFDPNQILKNPFAFDMQEALKSNIASCQTIFTEPMPLVKG
                     LDFFEQQLSPFRIYPLLTNAVQTADCEPDQFCASIAIFCSRPDAAKIYASEIRIALSM
                     ETSRDQPFSGDCISFQDTGVDLNTYATNFERFFNMLGLTDVVIHEEPRYYEYEDEGGR
                     IYVNARGVSMPLNPNFAPVMPAHFLN"
     gene            complement(3388..5982)
                     /gene="clpB"
                     /locus_tag="ACZPB6_03605"
     CDS             complement(3388..5982)
                     /gene="clpB"
                     /locus_tag="ACZPB6_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR1183593.1"
                     /translation="MRQDKLTTKFQEALGEAQSLALAHDNQVIEPVHILAAVLADGEG
                     SSRSLLERAGVNVQSLTREVNAAVERLPKVSGTGGDVQISRDLMNLLNLTEKEAMKRG
                     DEFISTEMLLLALTEDRSEAGRLAKNAGLTRAAIEAAVETVRGGDKADNPDAESQREA
                     IKKYTVDLTERARQGKLDPVIGRDDEIRRTMQILQRRSKNNPVLIGEPGVGKTAVVEG
                     LAQRIVNNEVPDSLRGKRILSLDMAGLIAGAKYRGEFEERLKKLLKEVTAESGRIILF
                     IDELHTVVGAGKTEGSMDAGNMLKPALSRGELHVIGATTLDEYRKYVEKDAALERRFQ
                     KVLVDEPSVEDTIAILRGLQERYEAHHGVEITDPAIVAAAELSHRYITDRFLPDKAID
                     LIDEAAARVKMEIDSKPEALDKLDRRLIQLKIEREAMKKETDDASKKRLQAIEDEIDK
                     LSREYADLEEVWKKEKSEALGAKSVRDEIDRTRHQMEEYRRTAQYEKLAELQYAVLPK
                     LEERLKKAEKAEAGDAPKPKLLRTTVGAEEIAEVVSRATGIPVAKMMEGERQKLLHMK
                     DALEERVVGQDEAVKRVAETILRSRAGLSDPNRPYGSFLFLGPTGVGKTELTKALAQF
                     LFDTEDALVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRHPYSVILLDEV
                     EKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSSEIQEMIGESRESVK
                     NAVMTEVREHFRPEFINRIDEIVIFNSLGSEAIRRIAAIQLKSLQARVAAQDVTLEVT
                     DEAMAELAEVGFDPHYGARPLKRAIQDHIENRLAMVMLEGSAGPGDTIRVGAKDGRFT
                     FDVEKAAQ"
     gene            complement(6219..6611)
                     /gene="crcB"
                     /locus_tag="ACZPB6_03610"
     CDS             complement(6219..6611)
                     /gene="crcB"
                     /locus_tag="ACZPB6_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR1183594.1"
                     /translation="MTRDGPLWLVILAVGTGAGLGAVCRWMLSYFLNPLAGWMPWGTL
                     ASNAAGGLLIGAALAWTSANPELPPAVRLFVITGFLGGLTTFSTFSAEAFSLITSGAF
                     IRAGVHMASHLLLSLTGTWAGWTAVRSL"
     gene            complement(6608..7078)
                     /gene="moaE"
                     /locus_tag="ACZPB6_03615"
     CDS             complement(6608..7078)
                     /gene="moaE"
                     /locus_tag="ACZPB6_03615"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HIR1183595.1"
                     /translation="MSLTTIRVSEADFNPGEEIERLIESSPEAGGVASFVGVVRNRND
                     GNDVTHLTLEHYPAMTERALAGIVERARARWDVCGVTVIHRVGEMAVGEHIVLVVVTS
                     AHRREAFEACEFIMDWLKTEAPFWKKERTPEGERWVDARESDEEARRRWGEAAR"
     gene            complement(7081..7338)
                     /gene="moaD"
                     /locus_tag="ACZPB6_03620"
     CDS             complement(7081..7338)
                     /gene="moaD"
                     /locus_tag="ACZPB6_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HIR1183596.1"
                     /translation="MKIRILYFALLRDALGRAQEELEVPEDVTTVAGLREWLRGCGEP
                     YATALGNARRVRAAVNQEMAEPDDAVKAGDEVAFFPPVTGG"
     gene            complement(7392..8597)
                     /locus_tag="ACZPB6_03625"
     CDS             complement(7392..8597)
                     /locus_tag="ACZPB6_03625"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR1183597.1"
                     /translation="MITYAEALRRLMEKAVPVEGMETVPLLYSTHRVLAEDIVSPIAV
                     PGWDNSQMDGYAVRAAELAEASEDHPVVLPVSDRIPAGQVGSRLAQGSVARIFTGAPM
                     PEGADTVVPQENVEVTPEGVCFKSPVREGAWVRRRGSDVPQGGVVLKKGARLTPAAIG
                     MVASIGRAYVKVYRRLRVGIFFSGDELVQPGEPLPPGGIYNSNRFMIRSLLQELGCEA
                     LDLGSVPDTLEATIEAFESAAASADVILTTGGMSVGEEDHIKPAVEAVGSLNVWRVRL
                     KPGKPLAFGEVRGVPFIGCPGNPVAGFVVFLMMARPYLLRTMGLTDIDVKPMAVRADF
                     DWLKPGDRQEFVRVRRNDEGGLDLYHTQNSQVLSSCAWADGLVDIPVGGTVRKGDMVS
                     YYPFCQFFA"
     gene            complement(8594..9082)
                     /gene="mobB"
                     /locus_tag="ACZPB6_03630"
     CDS             complement(8594..9082)
                     /gene="mobB"
                     /locus_tag="ACZPB6_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIR1183598.1"
                     /translation="MPKTVGFVGFSESGKTTLATRVAAELTRRGFRVAALKDAHHGFD
                     MDKPGKDSWRYREAGASQVIVRSDRRWAMLVETPDQPSVEELLARFSDVDVILVEGFK
                     NEGAFPKIEVRRRACEANPRLSLMHDDVVAVASDFEEPDERVPRLDINDPAAVADFVV
                     SL"
     gene            complement(9075..10511)
                     /gene="thrC"
                     /locus_tag="ACZPB6_03635"
     CDS             complement(9075..10511)
                     /gene="thrC"
                     /locus_tag="ACZPB6_03635"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR1183599.1"
                     /translation="MQYISTRGLESSFSFSDILFEGYAEDGGLYVPAVFPKVDAHTLM
                     TWRGLSYASLAFEVIRLFAEELPKEDLWQICSEAYQAGNFGHTRGMFTAEEITPVKWL
                     HDQVGLLELSNGPTLAFDDIALQFMSRLFDTSFAQGRPRTLLGATTGDMGAAAEAAFG
                     GHKNIKVVMLSPKDRMSQFQAAQLYSNQDPNVVNIEVNGTFDECQDIVRTILEDREYA
                     KAHGLGAINSVLWARIVPQIVYYFYGYLRAVERVGEEVVFAVPGGNFGNAYACWVAKK
                     MGLPIMRLIVCTNENDAMDEFLRTGRYAPKPSSETIATSSPSTDISRAASFERFLFDV
                     LGRDGTRVRELAEDLKTKGEFTLTAEEFRKVRLSGVVSGSSNHPNRLEIIEQMHLEYD
                     TYVDPHTADALFSGIYLHPVGVKTLCVETVQAVKFPRMVRQATGVAVEPPEDMADLFS
                     RPLRKVQTPACEAAVREEIERLTERPDA"
     gene            10800..>11184
                     /locus_tag="ACZPB6_03640"
     CDS             10800..>11184
                     /locus_tag="ACZPB6_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR1183600.1"
                     /translation="MPLPKRPAKRGDVLTDYGPEADLTKPAGKAAPKAAAKPSQAPET
                     ASKPRRTTRKTVKAALAETTKTVEKTVTAQETAAPKSTAKTPAKPAVKKPLVKKAATA
                     VKPQTAPAVAESDVRESRHSNRRGRA"
BASE COUNT         1969 a         3843 c         3285 g         2087 t
ORIGIN      
        1 ttttttttgc gcccgttttc ttcaccctgc caggccccct tctcgatgac gcgcccgccg
       61 cgcccccgcc cggcgaacaa tcaatataag aaagggtccc ccttatgttc agcaatccca
      121 ctgcgatcac cttcgtcggc tatctgctcg tcatggtgtg cgtcggcttc tacgcctact
      181 accacacccg cagctacggc gactacattc tcggcggacg tagcctcggc ggcttcgtga
      241 cggccctttc ggtcggcgcc tccgacatgt ccggctggct tctgatgggc ctgcccggcg
      301 ccgtctacct cgccggcatc tcccagtcgt ggatcggcat cggcctcgtc ctcggctcct
      361 acatcaactg gtgcgtcgtc gcctcccgcc tgcgcgtcta cacggagaag acccacaacg
      421 cgctcacgct ccccgactac ttctcgcacc gcttcgagga caagcaaaac ctgctgcgca
      481 tcatcgccgc cgtcgtgatc ctgctcttct tcacgatcta ctgcgcctcg ggcgtggtcg
      541 cgggcgcccg cctcttcgag aacaccttca gcatgtccta tgagacggcc ctcattctgg
      601 gcgccgccgc cacgatctgc tacgtcttcg tgggcggctt cctcgccgtc tcctggaccg
      661 acacgattca ggctctcctc atgtgcctcg ccctgatcgt gacgccgatc gccgtcatgt
      721 acgacctcgg cggcttcacc gccgccaccg agtgcgtcgc gagcgtcgac atcaagatgc
      781 tcaacatcct cgagggccac accctcgtgg gcatcgtcag ccttctcgcc tgggggctgg
      841 gctacttcgg ccagccgcac atcctcgtgc gcttcatggc cacccgctcc atcaaggtga
      901 tcccgaacgc ccgccgcgtc gccaccctct ggatgagctt ctgcctctcg ggcgccgtcg
      961 ccgtcggctt cttcggcgcg gccttcttcg ccgagcaccg cgacgtcgcc tccctcgtcg
     1021 aacagaacca cgagcgcgtc ttcatcgtcc tctcgggcct tctcttcaat ccctggatcg
     1081 ccggcatcct aatgtccgcc attctcgcgg ccgtcatgtc cacgctctcc tgccagctgc
     1141 tcgtgtgctc gtcgactctc accgaggact tctaccgcac gttcatccgc cgcaacgcct
     1201 cccagcgcga actcgtctgg ttcggccgca gcatggtgct cctcgtctcg gtcgtcgccg
     1261 tctggctcgc ccgcgacccc aacagcctcg tgctctccct cgtctcctac gcctgggcgg
     1321 gcttcggcgc ggccttcggc cccgtcatca tcatgtcgct ctggtggcgc cgcatgacgc
     1381 gcaacggcgc gctcgcgggc atgatcaccg gcgccgtcac ggtcctcgtc tggaaccact
     1441 acgcctggct cggcctctac gaaatccttc cgggcttcgt cttcgccctc atcgccatcg
     1501 tcgtcgtgag ccttctcgac aaggctccct ccaagagcat cgcgaagacc ttcgacgaag
     1561 tccaggccgc cgtgcgcaat cccgcctgac cgcttcaggc ctgccacgat tgaaaagagg
     1621 agcctcgcgc tcctcttttc acatccgacc cgaatgcagt ccgtgcaggc caaatccgtt
     1681 agaatctcct gcacaacgct tttcggggaa gccgccgcag cggccgtccc catcccgctt
     1741 tccgcggcgc ccgcaaccgg ctgccgcatg accttcattc atggcacgct cttcacatct
     1801 tgcggcccag cgccgcgaac tcagcttcat cctcaaccgc ctgacggcct atacgaccct
     1861 cgccgaccgt gacatcctcg agcagcgtct gcgcacgatc ctcgagaagc cgctctccga
     1921 cgacttctac atcatggcca acgaagtctt cgacaagatc gaggcccagc atccttccgt
     1981 cgccaacgag gcccgcggga acttctacgg catgctctcc gaggtgaagt acgaatacag
     2041 gaccccgacc gagtccacat cgatgctgat ggtcggcctg atgatgagca cgttcttcaa
     2101 gaccgcctgc cccaacgtca cgctctcgca ggaaaccgcc gacaaggttg tcgagatcct
     2161 caagaagcac tacatcaacc cgaaggccaa gatcacgatc ttccatgaaa tgctccgcag
     2221 caatcaaggg cccgccaacg ataccgagac ggcgggcagg ctcacgcgtg cgatggccga
     2281 atgcaaggga accctctaca ccaaggaaca cctcgacgta cccgaaggcg aggatgaccc
     2341 catcgccgcg cttgagaacg aacgcatcta cggcctctac ctgcgccacc tcgtcatcac
     2401 cgtaaccgtg cctgcgggcg agctcgccgt cgtgcatccc taccgctggt gggacaccac
     2461 gcgccacccc gagttctgcg tcagcggcca cttcgatccc aaccagatcc tcaagaatcc
     2521 gtttgcgttc gacatgcagg aggccctcaa gtccaacatc gcgtcctgcc agacgatctt
     2581 cacggaaccc atgcccctcg tcaagggact cgacttcttc gagcagcagc tctcgccctt
     2641 ccgcatctac ccgctcctta caaatgccgt gcagacggcg gattgcgagc ccgaccaatt
     2701 ctgcgcctcg atcgccatct tctgctcgcg tcccgacgcg gccaagatct acgcctccga
     2761 aatccgcatt gcactctcca tggagacctc ccgcgaccag cccttctcgg gcgactgcat
     2821 ctccttccag gacacgggcg tcgacctcaa cacatacgcc accaacttcg aacgcttctt
     2881 caacatgctc ggcctcaccg acgtcgtcat ccacgaagag ccccgctact atgaatacga
     2941 ggacgagggc ggccgcatct acgtgaacgc ccgcggcgtc tccatgccgc tcaacccaaa
     3001 cttcgccccc gtcatgcccg cacacttcct caactgacgc gcggcacatg caaaaagacg
     3061 gccctgcgct tcctgcgcga gaccgtcttt ttcacgaacc ccctccccct acacttgcaa
     3121 acatggagct gaccatgtat tgccaacccg agcaccctcc tgcttggcac catggcctct
     3181 gctgacttct cacggcaagc tttactccat cgccctgttg gggcgatgtc cgtgaggcct
     3241 cctcgggcaa ggacacgctc actcccctca tgcagctgcc tcattgcgca tcgcgacgca
     3301 cacactgcga aggggcttcg ccgttgccgg cgaagcccct ttcgtcatga gcagctcacg
     3361 agtcggacgg atccgagcac ctcagcgtca ctgagcggcc ttctccacgt cgaacgtgaa
     3421 cctgccgtcc ttcgcgccca cgcggatcgt gtcgccggga cctgcggaac cctcgagcat
     3481 gaccatcgca agacgattct cgatgtggtc ctggatcgcg cgcttgagcg gacgtgcgcc
     3541 gtaatgcgga tcgaagccca cttcggcgag ttccgccatg gcctcgtcgg tcacctcgag
     3601 cgtcacgtcc tgggcggcca cgcgggcctg caggctcttg agctggatcg cggcgatgcg
     3661 acggatggcc tcgctgccaa gcgagttgaa gatcacgatc tcgtcgatgc ggttgatgaa
     3721 ctccggacgg aagtgctcgc gcacctcggt catcacggca ttcttcacgc tctccctcga
     3781 ctcgccgatc atctcctgaa tctcggagga gcccagattc gaggtcatga cgatcacggt
     3841 gttcttgaag tccaccgtgc ggccctggcc gtcggtcatg cggccgtcgt caagcacctg
     3901 cagcaggacg ttgaagacat cgggatgagc cttctcgact tcgtcaagaa gaatcacgct
     3961 gtagggatga cggcggacgg cctccgtcag gtagccgcct tcctcgtaac ccacgtatcc
     4021 cggaggcgcg ccgatcaggc gggcgacgct gtgcttttcc atgaactcgc tcatgtcgat
     4081 gcgcacgagc gcatcctccg tgtcgaagag gaactgcgcg agcgccttcg tgagctcggt
     4141 cttgccgacg cccgtcgggc cgaggaagag gaagctgccg taaggacggt tcggatccga
     4201 aagccctgca cggcttctca gaatcgtctc ggccacgcgc ttgacggctt cgtcctgccc
     4261 cacgacgcgc tcctcgagcg catccttcat gtgcaggagc ttctggcgct cgccctccat
     4321 catcttcgcg acgggaatgc ccgttgcgcg actgacgact tcggcgattt cctcggcacc
     4381 cacggtggtt ctgagaagct tcggcttggg cgcatcgccc gcttcggcct tctcggcctt
     4441 cttcaggcgt tcctcaagct tgggcagaac ggcgtactga agctcggcga gcttttcgta
     4501 ctgggccgtg cggcggtact cctccatctg gtgacgcgtg cggtcgatct cgtcgcggac
     4561 gctcttggcg ccgagcgcct cgctcttctc cttcttccag acctcctcaa ggtcggcata
     4621 ctcgcgggag agcttgtcga tctcgtcctc aatcgcctgc agacgcttct tcgaggcgtc
     4681 gtcggtctcc ttcttcatgg cctcgcgctc gatcttgagc tggatcaggc ggcggtcaag
     4741 cttgtcgaga gcctcgggct tggaatcgat ctccatcttc acgcgggccg cggcctcgtc
     4801 gatcagatcg atggccttgt cgggcaggaa gcggtcggtg atgtagcggt gcgaaagttc
     4861 ggccgcggcc acgatggctg ggtcggtgat ctcgacgcca tgatgcgcct catagcgttc
     4921 ctgcaggccg cgcagaatgg cgatcgtgtc ctcgacgctc ggttcgtcga ccaacacctt
     4981 ctggaagcgg cgctcgaggg cggcatcctt ctcgacgtac ttgcggtact cgtcaagagt
     5041 ggtcgcgccg atcacgtgca gctcgccgcg cgagagcgcg ggcttgagca tgttgcccgc
     5101 atccatcgag ccttccgtct tgccggcgcc caccacggtg tgcagctcgt cgatgaagag
     5161 gatgatcctg ccggactcgg ccgtcacttc cttcagaagc ttcttgagac gctcctcgaa
     5221 ctcgccgcgg tacttcgcac ccgcaatcag gcccgccatg tcgagcgaga gaatgcgctt
     5281 gccgcgaagg ctgtcgggca cctcgttgtt gacgatgcgc tgcgccagac cctcgaccac
     5341 ggccgtcttg cccacgccgg gctcgccgat caggacgggg ttgttcttgc tcctgcgctg
     5401 caggatctgc atcgtgcggc ggatctcgtc gtcacgaccg atcacgggat caagcttgcc
     5461 ctgacgggcg cgctcggtca ggtcgaccgt gtatttcttg atcgcctcgc gctggctttc
     5521 cgcatcgggg ttgtcggcct tgtcgccgcc gcggacggtt tcgaccgccg cttcaatggc
     5581 agcgcgagtc agccctgcat tcttcgcaag acggcccgcc tcgctcctgt cctccgtgag
     5641 ggcgagaagc agcatttccg tcgagatgaa ctcgtcgccg cgcttcatcg cctccttctc
     5701 cgtgaggttg aggaggttca tgaggtcgcg cgagatctgc acgtcgccgc ccgtgcccga
     5761 aaccttcggc aggcgctcga cggccgcgtt cacttcgcgc gtcaggctct ggacgttcac
     5821 gcccgcacgt tcaagaaggc tcctgctcga accttcaccg tcggcgagca cagccgccag
     5881 aatgtgcaca ggctcgatga cctggttgtc atgagcaagc gcgagcgact gggcctcgcc
     5941 caacgcttcc tggaatttag tcgttaactt gtcttgacgc atgttcttgt tccttcgaaa
     6001 acccctgcat gggaccgcat tgactctgcc tgcgatcacg ggattctcat ctgatgtccc
     6061 tacctatatg gggatagagc aggacatttc aagatgcctg gacattgaag ttgctacaga
     6121 gtttgtccgg cgtcaagact ctcggagagc cgaaatgaaa cgaccccgtc tggtgcaaga
     6181 ccggcggggc cgtgagcgtt caggctgcct tcccgtgctc agagcgagcg gacggccgtc
     6241 cagcccgccc aagtgcccgt caggctcagc agcaaatgcg aggccatgtg cacgccggcc
     6301 ctgatgaagg cgcccgacgt gatgagcgag aaggcttcag ccgaaaaggt cgagaaggtc
     6361 gtcagacctc cgagaaagcc cgtgatgacg aaaagccgta cggcgggcgg cagctcggga
     6421 ttcgcgctcg tccaggcgag tgcggcgccg atcagaaggc cgcccgcggc attggaggcg
     6481 agcgttcccc agggcatcca tcctgcgagc ggattgagga aataggagag catccatcgg
     6541 cagacggcgc cgagtccggc gcccgtaccg acggcgagga tcacgagcca gagcggcccg
     6601 tccctggtca tcgtgcggcc tcaccccagc gacgcctggc ctcttcgtcg ctctcgcgcg
     6661 cgtcgaccca gcgctcgccc tcgggcgtcc tctccttctt ccagaagggg gcctcggtct
     6721 tgagccagtc catgatgaat tcgcaggcct cgaaggcctc gcggcggtgc gcgctcgtga
     6781 cgacgacgag cacgatgtgc tcgcccacgg ccatctcgcc cacgcggtgg atgaccgtca
     6841 cgccgcagac gtcccagcgt gcccttgcgc gctcgacgat gcccgcgagc gccctttcgg
     6901 tcatcgcggg atagtgctcg agcgtgagat gcgtgacgtc gttgccgtca ttgcggtttc
     6961 tcaccacgcc cacgaagctc gccacgccgc ccgcttcggg agaggactcg atgagcctct
     7021 cgatctcctc gccgggattg aagtccgcct cgctcacgcg aatggtcgtc agggacatga
     7081 tcagcctccg gtcaccggcg gaaagaaggc gacctcgtcg cccgccttca cggcgtcgtc
     7141 gggctcggcc atctcctgat tgacggccgc gcgcacgcgg cgcgcattgc cgagcgcagt
     7201 cgcatacggc tcgccgcagc cgcgaagcca ctcgcgaagc cctgcgaccg tcgtcacgtc
     7261 ctcaggcacc tcaagctcct cctgcgcacg accgagggcg tcgcgcagaa gcgcaaagta
     7321 aaggatgcgg atcttcattt tgttatgtcc tctctttctt gtgtttcagg tttgcgcatt
     7381 tcgagccact ctcaggcgaa gaactggcag aacgggtagt agctgaccat gtcgcccttg
     7441 cggacggtgc cgcccacggg aatgtcgacg agaccgtcgg cccaggcgca ggaggaaagc
     7501 acctgcgaat tctgcgtgtg atagaggtcg agcccgccct cgtcgttgcg gcgcacgcgc
     7561 acaaactcct gacggtcgcc cggcttgagc cagtcgaagt cggctctcac ggccatgggc
     7621 tttacgtcga tgtcggtgag gcccatcgtc ctcaggaggt agggtctggc catcatcagg
     7681 aagaccacga agcccgcaac gggattgccc ggacagccta tgaagggcac gccgcgcacc
     7741 tcgccgaacg cgaggggctt gccgggcttg agacggacgc gccagacgtt gagcgaaccg
     7801 actgcttcga ccgccggctt gatgtggtcc tcttcgccca cgctcatgcc gcccgtcgtc
     7861 aggatgacgt cggccgaggc ggccgcgctc tcgaaagcct cgatcgtggc ctcaagcgtg
     7921 tcgggcacgc tgcccaggtc gagcgcctcg cagccgagct cctgaaggag gctgcggatc
     7981 atgaagcggt tcgagttgta gatgccaccc ggcggcaggg gttcgcccgg ttgcacgagc
     8041 tcgtcgcccg aaaagaagat gccgactcga agacggcggt aaaccttgac gtaggcgcgg
     8101 ccgatggacg cgaccatgcc gatcgcggcg ggcgtcaggc gggcgccctt cttgagcacg
     8161 acgccgccct gaggcacgtc ggagccgcgg cggcggaccc atgcgccctc gcgcacgggg
     8221 gacttgaagc agacgccctc gggcgtcacc tcgacgtttt cctggggcac gaccgtgtcg
     8281 gcaccctcgg gcatcggcgc gcccgtgaag atgcgcgcga cgctgccctg cgcaagcctt
     8341 gagccaacct gaccggcggg aatgcggtcg gaaacgggca gcacgacggg atgatcttcg
     8401 gacgcctcag ccagctcggc cgctctgacc gcgtagccgt ccatctggga gttgtcccat
     8461 ccgggaacgg cgatagggct cacgatgtct tcggcgagca cgcggtgggt tgaatagagg
     8521 agcggaacgg tttccattcc ctcgacgggc accgccttct ccatgaggcg gcgcagggct
     8581 tcggcgtagg tgatcatagg cttacaacaa aatctgcaac ggctgcaggg tcgttgatgt
     8641 cgagccgcgg caccctttcg tccggctcct cgaagtcgga ggcgacggcc accacgtcgt
     8701 catgcatgag cgagagtctc ggattggcct cgcacgccct gcgtcggact tcaattttag
     8761 ggaatgcgcc ctcgttttta aacccctcca caaggatgac gtcgacatcc gaaaatctgg
     8821 caagcagctc ttcaaccgac ggctgatcag gcgtttcaac gagcatggcc catcttcggt
     8881 cgctgcgcac gatcacctgc gaggcacccg cctccctgta gcgccagctg tccttgccgg
     8941 gcttgtccat gtcgaacccg tggtgcgcgt ccttaagcgc cgcgacccgg aaaccgcggc
     9001 gggtgagttc cgccgcaacc cgggtcgcaa gggtcgtctt gccgctttcg gaaaagccga
     9061 cgaacccgac cgttttaggc atcagggcgc tccgtcagcc tctcgatctc ctctcggacc
     9121 gccgcttcac acgcaggcgt ctggaccttg cgcagcggac ggctgaagag gtcggccatg
     9181 tcctcgggcg gctcgacggc aacgcccgtg gcctgcctga ccatcctcgg gaacttgacg
     9241 gcctgaaccg tctcgacgca gagcgtcttg acgccgacgg gatggaggta gatgcccgag
     9301 aagagcgcgt cggccgtgtg cgggtcgacg tacgtgtcgt actcgaggtg catctgctcg
     9361 atgatctcga ggcgattcgg atggttcgag gaacccgaca cgacgcccga caggcggacc
     9421 ttgcggaatt cctcggccgt gagcgtgaat tcgcccttcg tcttcagatc ctcggcaagc
     9481 tccctgacac gagtgccgtc gcggccgagc acgtcgaaga ggaagcgctc gaagctcgcg
     9541 gcgcgcgaga tgtcagtgga gggcgaggac gttgcgatcg tctcgctcga gggcttcggc
     9601 gcatagcgac ccgtgcgaag gaactcgtcc atcgcgtcgt tttcgttcgt gcagacgatg
     9661 aggcgcatga tcggaagccc catcttcttg gcgacccagc acgcgtacgc attgccgaag
     9721 ttgccgcccg gcaccgcgaa gacgacctcc tcgcccacgc gttcgacggc cctcagatag
     9781 ccgtagaagt agtagacgat ctgcggcacg atgcgggccc agaggacgga gttgatcgcg
     9841 ccgaggccgt gggccttcgc gtactcgcga tcctcgagga tcgtgcgcac gatgtcctgg
     9901 cattcgtcga acgtgccgtt gacctcgatg ttgacgacgt tcggatcttg attggaataa
     9961 agctgcgccg cctggaactg gctcatgcgg tccttcgggg aaagcatgac gaccttgatg
    10021 ttcttgtgcc cgccgaacgc ggcctcggcc gcagccccca tgtcgcccgt cgtggcgccg
    10081 agcagcgtgc gcgggcgccc ctgggcgaac gacgtgtcga agagcctcga catgaactgc
    10141 agggcaatgt cgtcgaaggc aagcgtcgga ccgttcgaaa gctcgagcag gccgacctgg
    10201 tcgtggagcc acttcacggg cgtgatctcc tcggccgtga acataccgcg ggtgtggccg
    10261 aagttgccgg cctgataggc ttcggagcag atctgccaga gatcctcctt cgggagctcc
    10321 tcggcgaaga ggcggatgac ctcgaaggcg agcgaggcat aggagaggcc gcgccacgtc
    10381 atcagggtgt gggcgtcgac cttcgggaac accgcaggca cgtagaggcc gccatcctcg
    10441 gcatagccct cgaagagaat gtcgctgaac gagaagctcg attcgaggcc tcgggtggaa
    10501 atgtactgca ttaaacctcc ttgaaaaggg ggaaatagtc gctgtcactc atcaggcaaa
    10561 tgacgataaa gccatactac ctgaaaatca ttctccgaaa acttaaagag acggtttttt
    10621 acggaaccca ggcatttgac ctacagcgaa ataggtgctt tcgagcaaat gctcacgcga
    10681 aacacccgtc cactcgatcg taattgtaag tcttgtcgac cttcagacag aagggggacg
    10741 cccgccggcc accgaacctc tacaatgact tcattgacaa tagattagag agaaacgcca
    10801 tgccgcttcc gaaaagaccc gccaagcgag gggacgtgct gaccgactac ggtcccgaag
    10861 ccgatctgac caagcccgcc ggaaaggctg cgcccaaggc cgcagccaaa ccgtcgcagg
    10921 ctcccgagac ggcttcgaag ccccgccgca cgacgaggaa gaccgtgaag gccgcgctcg
    10981 ccgaaacgac gaagactgtc gaaaagaccg tcaccgcgca ggagactgcc gcccccaaat
    11041 cgacggcgaa gacgcccgcg aagcccgccg tgaagaagcc cctcgtcaaa aaagccgcaa
    11101 ccgcagtgaa gccccagacg gcccccgcag tcgccgagtc cgacgtgcgc gaatccaggc
    11161 actccaaccg ccgcggacgc gccg
//
LOCUS       DBMDTI010000043        10955 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1778_length_10955_cov_3.197070, whole genome shotgun sequence.
ACCESSION   DBMDTI010000043 DBMDTI010000000
VERSION     DBMDTI010000043.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 10955)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10955)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10955
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1778_length_10955_cov_3.197070"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="ACZPB6_03645"
     CDS             <1..120
                     /locus_tag="ACZPB6_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HIR1183601.1"
                     /translation="DLGISIKTVEAHRASIMDKTNSGTVADLMRVVMGTKLLH"
     gene            288..1142
                     /gene="folD"
                     /locus_tag="ACZPB6_03650"
     CDS             288..1142
                     /gene="folD"
                     /locus_tag="ACZPB6_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR1183602.1"
                     /translation="MTAKIIDGKALAARIQDELKTRVEACTEHGHRPGLAVVLVGEDP
                     ASQVYVRNKVRACERTGIRSDEIRMPAETTEAELLAVIDRLNHDDAVDGILVQLPLPK
                     HLSSELVIAAISPEKDVDGFHVVSAGSLLTGRTGFRPCTPYGVMKLLEEAGCDPAGKH
                     AVVIGRSNIVGKPQALMLLEKNASVTIVHSRTTDLAEHTRRADILVAAVGRAKMVKAD
                     MVKPGAVVIDVGMNRDENGKLCGDVDYENVRGVASAITPVPGGVGPMTIAMLMTNTVE
                     AVERRLGL"
     gene            1163..3199
                     /locus_tag="ACZPB6_03655"
     CDS             1163..3199
                     /locus_tag="ACZPB6_03655"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR1183603.1"
                     /translation="MTESVNPLLNASGLIDYASVRPEHVAPAVETLSKNLEETLARVT
                     APETPATWEAVAEPLEKATLAFTRGWGVVGHLQSVVDTPALRDAYNAALPAVTQLFID
                     LSQNEALFAKYKAMKASDGFASLPPVRRRIIERELRDFRLSGAELPEAERARVKVVGE
                     RLSMLSQKFSENLLDATNAWELVVTDETRLAGIPEDARALFAANAKSAGKEGWRITLH
                     FPSYLPVMQYADDRSLRETLYRAFSTRASEFDGGKYDNTPLISEILRLRREEAALLGF
                     ADYAESSLATKMADTPAEVIAFLRDLASRAKPFAEKNMAALRAFAADELDIADMQPWD
                     QAYASEKLRQARYSYSDQEVKQYLTEPTVFSGLFKLAETLYGIRIRPATASVWHSDVK
                     YFEVCRDDEVIASFYADLYAREGKRSGAWMDADRTRCVMDGVLQTPVAYLVCNFAQGV
                     NGRPATLTHDDVTTLFHEFGHCLHHLLTDQTEVAVSGISGVEWDAVELPSQFMENFAW
                     EWDVVKGLAKHVETGESIPESLFEKMLAAKNFQSGIACVRQVEFALFDMLLHTSFNPD
                     TDDVQDLLQDVRREVAVSFPPDYNRFPQSFSHIFAGGYAAGYYSYKWAEVLSADAFSA
                     FEETGVLNPETGARFRREILGRGSSRDAMENFIAFRGRRPEIDALLRHSGMTVN"
     gene            3279..4085
                     /gene="xth"
                     /locus_tag="ACZPB6_03660"
     CDS             3279..4085
                     /gene="xth"
                     /locus_tag="ACZPB6_03660"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR1183604.1"
                     /translation="MSNITLASWNVNSLKVRLPQLLDWLKTSGVDAVVLQETKLTDDL
                     FPAESIREAGYDVVFTGQKTYNGVALLSKRDVFLPPEDVLLGLPDFPDDHKRFVAATL
                     VTRLEEKAIRFIGGYFPNGMTPGSWKYLYKLDWIAALTRHLKDTLTQCPDLVLGGDFN
                     IAPEDADVWNPAERKDDILVSAPERAAFRELLKLGLSDSFRLVSQESARYSWWDYRMK
                     GFENNHGLRIDHLLVSEALKERVKAADIDTGPRGNLQPSDHAPATVTLEL"
     gene            complement(4094..4591)
                     /locus_tag="ACZPB6_03665"
     CDS             complement(4094..4591)
                     /locus_tag="ACZPB6_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183605.1"
                     /translation="MVWAIWGMLGTAFTMEGTTGTEGLGGLGMAALLTAPFWLAFVLW
                     PLFWIWRRVRDRQLWTEKVELLVHDPESSEPFGLEVLFGRDGVRVAVDEVNGVEGLSD
                     ALTGIPTRKPDEAAGIPFETYDAADLAAWGVAWLEVHPDGEGALAEFARWTDTLRHAD
                     NAARR"
     gene            complement(4771..6249)
                     /gene="ntrC"
                     /locus_tag="ACZPB6_03670"
     CDS             complement(4771..6249)
                     /gene="ntrC"
                     /locus_tag="ACZPB6_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HIR1183606.1"
                     /translation="MGDKEQNVQSAPVWVVDDDHAIRWVLSRALSKAGIECRLFEEAE
                     SVLKALEDSAPMVLVSDIRMPGVSGVDLLARVKARMPNLPVIIMTAYSDLESAVSAFQ
                     GGAFEYLAKPFDITKAVELIERAMKEAQNMTPAVEAEAEQPASEESESQSVPDLIGQA
                     PAMQEVFRAIGRLSHSLVTVLLTGESGAGKEVVARAIWRHSQRAAKPYIAINMAAIPR
                     DLLESELFGHEKGAFTGATAQRLGRFEQARGGTLFLDEIGDMPMELQTRLLRVLSNGF
                     FYRVGGHQPIKADVRIIAATNQNLEQRVKDGLFREDLYHRLNVIRLRLPPLRERPEDI
                     APLAEHFLRTEARALGVEAKRLTPEALARLEAFRFPGNVRQLENLCRWLLVMAPAVEV
                     RVEDLPEEIREASENAPARTAGASSEAAYGDWTALLEELVSAKLAAGESGIWAELFPD
                     FERTVIRTALNVLNGRRIEAAQRLGLGRNTITRKIQELGIEE"
     gene            complement(6298..7407)
                     /gene="glnL"
                     /locus_tag="ACZPB6_03675"
     CDS             complement(6298..7407)
                     /gene="glnL"
                     /locus_tag="ACZPB6_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HIR1183607.1"
                     /translation="MVESKSAAGADAPFAILDMMSATVLLTDTLGRVLWGNSASEVLL
                     GCSRRTFVGTDFRDRLAECDEWYAHLASGAAVESARVFGRLLRAHPGAQPDDVRVQAV
                     LTRLPEGWPACRDAGVLIEIVDVEETLHQDREWMNCELMDANRQLLRNLAHEIKNPLG
                     GIRGAAQLLESDLVREEDRECTSVIIGEADRLQALVDRFLAPYRQKAQCEEVNVHEVL
                     EHVRSLVAMEFPDGLSFVRDYDISAPNVRGDRARLTQVFLNIVRNAAEVMDGERAEGR
                     AAIVLRTRIVRDVMAGTERVRMALAVDIIDNGPGVPVDMQEKIFYPLVTGRAEGSGLG
                     LSLAQTFVRQAGGTITLESVPGRTVFRVLLPFSQS"
     gene            complement(7498..8910)
                     /gene="glnA"
                     /locus_tag="ACZPB6_03680"
     CDS             complement(7498..8910)
                     /gene="glnA"
                     /locus_tag="ACZPB6_03680"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR1183608.1"
                     /translation="MTSASDVLQMIKDNDVKFVDLRLTDTLGKEMHVTVPADCIDEDV
                     FELGQPFDGSSFIGWRGIEASDMLLMPDPDSARMDPFREVNTLILQCNVHEPKTGKDY
                     DRDPRSLGLRAEAYLKSTGIGDTAYFGPEPEFFIFDSVRWEHRMGKSFFEIDSEEGPW
                     ATGLKTEGGNLGYHNRVKGGYFPVSPVDSFADMRSEMCTLLEQQGVGVEIHHHEVGGA
                     GQCEIGTRFNTLVKRADWTQILKYTVHNVAAAYGKTATFMPKPIEGDNGSGMHVHQSI
                     WKDGQNLFAGNGYAGLSELALYYIGGVIKHARALNAITNPSINSYRRLVPGFEAPVKL
                     AYSSCNRSASIRIPHVNSPKARRVEVRFPDPTANPYLAFSALLMAGLDGIQNKIHPGE
                     AADKNLYDLPPEENAKIPTVCSSLDQALENLDRDREFLTRGGVFSNDFIDAYIAVKME
                     EVNRWRLAVTPIEFDMYYSL"
     gene            complement(9225..10397)
                     /gene="hemW"
                     /locus_tag="ACZPB6_03685"
     CDS             complement(9225..10397)
                     /gene="hemW"
                     /locus_tag="ACZPB6_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR1183609.1"
                     /translation="MSAQSSWTAEAVANIPLSLYLHWPWCVRKCPYCDFNSHAAPADL
                     AEREYVDIVLKDLEGWLSVAGGRRIETVFIGGGTPSLMSGDAVARLLEGADGLFGFTS
                     DCEITLEANPGTVDEGRFRAFRDAGVNRVSVGVQSFMNEKLRKLGRIHVSEEALRAAA
                     HAGEVFGNFNLDLMFALPGETLEEVAREVETAVVTGATHLSFYQLTIEPGTAFAKRVP
                     EDLPDDDLCADMSDLVIRSLEEAGFEHYEVSGYAGPGCRCRHNLNYWTFGDYLAAGAG
                     AHGKVTTQDGVVREVRHASPRKYMASVSETGSGIAERRAVESGDLPFEFMLNALRLTE
                     GVPAELFEARTGLSLKVVEPILSDLRVQGLLAADELVIRPTARGRAFLSDLQEAFL"
     gene            complement(10397..>10955)
                     /gene="rdgB"
                     /locus_tag="ACZPB6_03690"
     CDS             complement(10397..>10955)
                     /gene="rdgB"
                     /locus_tag="ACZPB6_03690"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR1183610.1"
                     /translation="LREFQAMFAEFGVDVINQGALGVEACPEPFGTFIENCLAKARHA
                     SKMTGRPAMADDSGVCVNALNGMPGVLSARFAGEPSNDEANNRLLVERLQGKDDRRAH
                     YTCVLTAVRTPDDPEPLVAVGRWYGEICDEPAGEGGFGYDPHFFVPEYGRTAAELTAE
                     EKNRVSHRGKALVQMHKLLREAWGW"
BASE COUNT         2185 a         3664 c         3082 g         2024 t
ORIGIN      
        1 gacctcggca tctccatcaa gaccgtcgaa gcccaccgag cctccatcat ggacaagacc
       61 aactcgggca cggtcgccga cctcatgcgc gtcgtcatgg gtacgaagct gctccactga
      121 gcatcaattg ccgatacact gtgggatgtg cgcccgagga tcagccggaa acggctcccg
      181 cgggcggcct tcccgccatc aaggcctctc cgcctcaacg gcgcgggagg cccttttgtt
      241 ttttgaattc gacagaacat tgtcagctgt ggaccggagg tttcttaatg acagccaaga
      301 tcattgacgg caaggcactt gccgcacgca tccaggacga actcaagacc cgcgtcgagg
      361 catgcaccga acacgggcac cgtcccggac tcgccgtcgt cctcgtgggc gaggatcccg
      421 ccagccaggt ctacgtccgc aacaaggtcc gcgcctgcga gcgcacgggc atccgctctg
      481 atgaaatccg catgcccgcc gaaacgaccg aagccgaact cctcgccgtc atcgacagac
      541 tgaaccatga cgacgccgtg gacggcatcc tcgtgcagct tccgcttccg aagcacctct
      601 cctccgaact cgtcatcgcc gccatctctc ccgaaaagga cgtcgacggc ttccatgtcg
      661 tctccgccgg ctctctcctc acgggtcgca ctggttttcg tccgtgtacg ccctacggcg
      721 tcatgaagct tcttgaggaa gcggggtgcg atcccgccgg caagcatgcc gtcgtgatcg
      781 gccgctccaa catcgtgggc aagccccagg cgctgatgct tctcgaaaag aacgcctccg
      841 tcacgatcgt gcacagccgc acgacagatc tggccgagca cacccgccgc gccgacattc
      901 tcgtcgccgc cgtcggccgc gccaagatgg tcaaggctga catggtgaag cccggcgccg
      961 tcgtgatcga cgtcggcatg aacagggatg aaaacggcaa gctctgcggc gacgtcgact
     1021 acgaaaacgt ccgcggagtc gcatccgcca tcactcccgt cccgggcggc gtgggcccca
     1081 tgacgatcgc catgctgatg accaacaccg tcgaggcggt cgaacgccgc ctcggtctct
     1141 gatgacaggg agacgcaccg ccatgaccga atccgtcaat cccctgctca atgcgtcggg
     1201 cctgatcgac tacgcctccg tccgtcccga gcacgtggcc cccgccgtcg agacgctatc
     1261 gaagaatctt gaggagacgc tcgcacgcgt cacggcaccc gaaacgcccg ccacgtggga
     1321 ggccgtcgcc gaaccgctcg aaaaggcgac actcgccttc acgcgcggct ggggcgtcgt
     1381 cggccacctg cagagcgtcg tcgacacgcc tgcgttgcga gacgcctaca atgccgcgct
     1441 tcccgccgtc acgcagctct tcatcgatct gtctcagaac gaggcgctct tcgccaagta
     1501 caaggcaatg aaggcctccg acggcttcgc ctctctcccg cccgtccgac gccgcatcat
     1561 cgagcgtgaa ctccgcgact tccgcctctc gggcgccgag ctgcccgaag ccgagcgcgc
     1621 acgcgtcaaa gtcgtcggcg aaaggctctc catgctgagc cagaagttca gcgaaaacct
     1681 gctcgacgcc accaatgcct gggagcttgt cgtcacggac gagacgcgcc ttgccggcat
     1741 tcccgaggat gccagggcgc tcttcgccgc caacgcgaag tccgcaggaa aggaaggctg
     1801 gcgcattacg cttcacttcc cgtcctacct gcccgtcatg cagtacgcgg atgaccgctc
     1861 gcttcgcgag accctctacc gagccttctc gacccgagcc tccgaattcg acggcggcaa
     1921 gtacgacaac accccgctca tcagtgaaat cctcaggctt cgccgtgaag aggcggctct
     1981 tctgggcttt gccgactatg cggagtcgtc gcttgccacc aagatggccg atacccctgc
     2041 cgaagtcatc gcctttcttc gtgatctcgc ctcccgcgcc aagccctttg ccgaaaagaa
     2101 catggcggcc cttcgtgcct ttgctgctga tgagctcgac atcgccgaca tgcagccgtg
     2161 ggatcaggcc tatgcttccg aaaagctgcg tcaggcccgc tacagctact ccgatcagga
     2221 ggtaaagcag tacctcaccg aaccgacggt cttctcagga ctcttcaagc ttgcggaaac
     2281 gctctacggc atccgcatcc gtcccgccac ggccagcgtc tggcactccg acgtcaagta
     2341 ctttgaagtc tgtcgcgacg acgaggtcat cgcatccttc tacgccgacc tctatgcgcg
     2401 agaaggcaag cgctccggcg cctggatgga cgccgaccgc acgcgctgcg tcatggacgg
     2461 cgtcctgcag acgccggtcg cctatctcgt ctgcaacttt gcccagggcg tcaacggccg
     2521 ccccgccacg ctcacgcatg acgacgtgac gacgctcttc cacgaattcg gccactgcct
     2581 ccaccatctc ctcacggatc agacagaggt cgccgtctcg ggcatcagcg gcgttgaatg
     2641 ggacgccgtc gaacttccga gccagttcat ggagaacttt gcctgggagt gggacgttgt
     2701 caagggactt gcgaagcatg tcgagacggg cgagtccatt cccgagtcac tttttgaaaa
     2761 gatgctcgcc gccaagaact tccagtccgg catagcatgc gtgcgacagg tcgagtttgc
     2821 actcttcgac atgctcctgc acacgagctt caatcccgac acggatgacg ttcaggacct
     2881 tctgcaggac gtgcgccgag aggttgccgt gagcttcccg cccgactaca accgcttccc
     2941 gcagagcttc tcccacatct ttgccggcgg ctatgccgca ggctactaca gctacaagtg
     3001 ggccgaagtc ctctccgccg acgcgttctc cgcctttgag gagacgggcg tgctcaatcc
     3061 cgagaccggc gcacgcttcc gaagggaaat cctcggacgc ggctccagcc gcgatgcgat
     3121 ggaaaacttc atcgccttcc gtggccgccg tccggaaatc gacgccctgc tgcgccacag
     3181 cggcatgacc gtcaactgac cgacgccgaa acggcccctt cttccaaacg gcggaggggc
     3241 cgccgttttt tcccatcttc atccagaaaa ggtgcccctt gtccaacatc acgctagcca
     3301 gctggaacgt caactccctg aaggtccgcc ttccccagtt gctcgactgg ctgaagacct
     3361 caggcgtcga cgcggtcgtc ctgcaggaga cgaagctcac cgacgaccta ttccccgccg
     3421 aatccatcag agaggcgggc tatgacgtcg tcttcacagg ccagaaaacc tacaacggag
     3481 ttgcgctcct ttcgaaacgc gatgttttcc tgcctcccga ggatgtgctt ctcggacttc
     3541 ccgactttcc cgacgaccac aagcgcttcg tggccgccac acttgtaacg cgactcgagg
     3601 agaaagcgat caggttcatc ggcggctact tcccgaacgg catgacgccc ggaagctgga
     3661 agtacctcta caagctcgac tggatcgccg ccttgacgag gcatctcaag gacacgctga
     3721 cgcaatgccc cgacctcgtc cttggcggcg acttcaacat cgcccctgaa gacgccgacg
     3781 tatggaaccc tgccgagcgc aaggacgaca ttctcgtgag cgctcccgag cgcgccgcat
     3841 tcagggaact gttgaagctg gggctttccg acagcttcag gctcgtctct caggaatccg
     3901 cccgctattc atggtgggat taccgcatga aaggctttga aaacaaccac ggacttcgca
     3961 tcgaccatct tctggtctcc gaagccctga aggagcgcgt caaagccgca gacatcgata
     4021 cggggccgcg tggcaacctg caaccctccg accatgctcc cgcaactgtg acgctcgagc
     4081 tttgatcagc cgctcagcgg cgtgccgcat tgtcggcatg ccgcagagtg tcggtccagc
     4141 gtgcgaattc ggccagcgct ccctcgccgt caggatgcac ctccagccat gccacacccc
     4201 aggcggcgag gtccgcggca tcgtacgttt caaagggaat ccccgccgct tcgtcaggct
     4261 tccttgtcgg aattcccgtc agggcatccg aaagtccttc aacgccattc acctcatcca
     4321 ccgccacgcg cacgccgtct ctgccgaaga gcacctcaag cccgaagggc tcggaacttt
     4381 cgggatcatg caccaacagc tccaccttct ccgtccaaag ctgacggtct cgaacacgtc
     4441 gccatatcca gaagagaggc cagagcacaa acgccagcca gaagggagca gtcagcagag
     4501 cagccatgcc cagccctcca agcccctcgg ttcctgtcgt cccctccatc gtgaatgcag
     4561 tgccgagcat gccccatatt gcccacacca tccaggcgcg ccctgccctt gttcctgaca
     4621 ttcggcagcg aagcaccttc ctccaatcgt tcatgatcct cctcgacagt tgaccaaccc
     4681 ggccctcccc tgctttcgtg catgccggcc gtcctgcgca tcataccgac acgcgtgcag
     4741 accgttcatc gcaacacgcg caaaccagcc tcactcctcg attcccagtt cctgaatctt
     4801 tcgcgtgatg gtgttgcgac caagccccag acgctgtgcg gcttcgatcc tgcgaccgtt
     4861 cagcacgttg agcgccgttc ggatgaccgt tctctcgaag tcggggaaga gttcggccca
     4921 tatgccgctc tcccctgccg caagcttcgc cgacacgagt tcctccagaa gagccgtcca
     4981 atcgccatag gccgcttccg aagaagcccc ggccgtcctt gcaggtgcat tctcggacgc
     5041 ttccctgatt tcctcgggaa ggtcctcgac acgtacctcg acggcgggcg ccatcacgag
     5101 aagccatcgg cagagattct caagctgtcg aacattgccg ggaaagcgaa acgcctccag
     5161 acgcgcaagt gcctccggcg tcaggcgctt cgcctccacg ccaagcgccc gcgcctcagt
     5221 gcgcaggaag tgttcggcca gaggcgcaat gtcttccgga cgttcacgca ggggcggcag
     5281 acgcagccga atcacgttca gacgatggta gagatcctct cgaaaaaggc cgtcctttac
     5341 acgttgttcc agattctgat tggtggcggc gatgatgcgg acatccgcct tgatcggctg
     5401 atgaccaccc acgcgataga agaagccgtt gctcagaacg cgcagaagcc tggtctgcag
     5461 ttccatcggc atgtcgccga tttcatcgag aaagagcgtg ccgcccctcg cctgctcaaa
     5521 acgaccaagg cgctgtgcgg tagcccccgt gaatgctccc ttttcatggc cgaaaagctc
     5581 gctctccaac agatcacgcg ggatggccgc catattgatg gcgatgtagg gcttcgccgc
     5641 cctctggctg tggcgccaga tggccctggc cacgacctcc ttgcccgccc cggactcacc
     5701 tgtcaaaagc accgtcacaa ggctgtggga aagacgcccg atcgcgcgaa agacctcctg
     5761 catcgcaggc gcctgaccga tgaggtcggg aacggactgc gactcagact cttccgatgc
     5821 aggctgctcc gcctccgctt ctacggctgg cgtcatgttc tgagcctcct tcatggcgcg
     5881 ctcgatgagc tcgaccgcct tcgtgatgtc gaagggcttt gccagatact cgaaggcccc
     5941 gccctgaaag gccgagacgg cgctctcaag atccgaatag gccgtcatga tgatgacggg
     6001 gaggttaggc attctggcct tgacacgagc cagcagatcc acccccgaca ctcccggcat
     6061 gcggatgtcg ctcacgagca ccatgggcgc agagtcttca agcgccttca gtacggactc
     6121 cgcctcctcg aaaagcctgc attcgatgcc cgccttggaa agcgcccgtg aaagcaccca
     6181 ccggatggca tgatcgtcat caacaaccca gacaggggcg ctctgtacat tctgttcctt
     6241 gtcacccata ggacttctct cgctcactac ttgaattctt tctcagatca ggaaccgtca
     6301 agactgcgaa aacggcagca gcactcggaa gacggtccgc ccgggaacgc tctcgagcgt
     6361 gatcgtacca cccgcctgcc tcacgaaggt ctgggccaga ctgagtccca gccccgatcc
     6421 ctcggccctc cccgtcacca gcgggtagaa gatcttctcc tgcatgtcga cgggaacgcc
     6481 ggggccgttg tcaatgatgt cgacggcaag cgccattcgg acacgctccg ttcccgccat
     6541 cacgtcccgc acgatgcggg tcctcagtac aatggccgcc cgcccctccg cacgctcccc
     6601 gtccatgact tccgccgcgt tgcggacgat gttgagaaac acctgcgtga gtctggcccg
     6661 gtcgcctctc acattcggcg ccgaaatgtc atagtcgcgc acaaaggaga gtccatcggg
     6721 gaactccatc gcaaccaggc ttcttacgtg ctccagcacc tcatgaacgt tgacctcctc
     6781 gcactgcgcc ttctggcgat agggcgcaag gaagcggtct accaaagcct gcagacgatc
     6841 ggcctctcca atgatgacgc tcgtgcattc ccgatcctcc tcacgaacga ggtcggactc
     6901 aaggagctgc gccgcaccgc ggatgccgcc cagaggattc ttgatttcgt gcgcaagatt
     6961 tcgaagaagc tgcctgttgg cgtccatcag ctcgcagttc atccactccc tgtcctgatg
     7021 aagcgtttcc tcgacatcaa cgatttcaat cagaacaccg gcgtccctgc aggcgggcca
     7081 gccttcgggc aggcgcgtca gcacggcctg aacgcggacg tcgtcaggct gagcgccggg
     7141 atgcgccctg agcagacgcc caaacacgcg ggcggactcc acggccgccc ccgacgccag
     7201 atgcgcatac cactcgtcac actccgcgag acggtcgcga aagtccgtac cgacaaacgt
     7261 tcgcctcgaa catcccagca gcacttcgga cgcactgttg ccccagagca cccttcccaa
     7321 agtatcggtc agcagcaccg tggcggacat catgtcgaga atggcaaacg gggcgtccgc
     7381 ccctgctgcg cttttggact caaccatgga cggttctccc aaacaaaaag gcgggcgacg
     7441 gcttgaaaat cgagccgcca cccgcctgtc aggtctgcgt cactccgacg gctgaggtca
     7501 gaggctgtag tacatgtcga attcgatcgg cgtcacggcg aggcgccaac ggttgacctc
     7561 ctccatcttc acggcgatgt aggcatcgat gaagtcgttg gagaagacgc cgccgcgggt
     7621 caggaactcg cggtcacggt caagattttc gagcgcctga tcaagcgaag aacagacggt
     7681 cggaatcttg gcgttttcct caggcggcag atcgtagagg ttcttgtcgg ccgcttcgcc
     7741 cggatggatc ttgttctgga tgccgtcgag acctgccatc aacagcgcgg agaaggcgag
     7801 gtagggattg gccgtcggat ccgggaagcg aacctcaacg cggcgggcct tcgggctgtt
     7861 gacgtgcgga atgcggatgg aagccgaacg gttgcaggac gagtaggcga gcttcacggg
     7921 tgcttcaaag ccgggcacga gacgacgata cgaattgatc gacgggttcg tgatggcatt
     7981 gagggcgcgg gcgtgcttga tgacgccgcc gatgtagtag agcgcaagct cggagagacc
     8041 ggcgtaaccg ttgcccgcaa agaggttctg gccgtccttc cagatggact gatggacgtg
     8101 catgccggaa ccgttgtcgc cctcgatggg cttgggcatg aacgtagccg tcttgccgta
     8161 ggcggcggcg acgttgtgaa ccgtgtactt gagaatctgc gtccagtcgg cgcgcttgac
     8221 gagcgtattg aagcgggtgc cgatttcgca ctggcctgcg ccgcccactt catggtgatg
     8281 gatttcaacg ccaacgccct gctgttcaag cagcgtgcac atttcagagc gcatgtcggc
     8341 aaaggaatcg acgggagaga cggggaagta gccgcccttg acgcggttgt ggtagccgag
     8401 gttgccgcct tccgtcttga gaccggtcgc ccaagggcct tcttcgctgt cgatttcgaa
     8461 gaagctcttg cccatgcggt gttcccagcg gacgctgtcg aagatgaaga actcgggttc
     8521 gggaccgaag taggccgtgt caccaatgcc cgtcgacttc agataggctt cggcgcgcag
     8581 gccgagggaa cgaggatcac ggtcatagtc cttgccggtc ttgggttcgt gcacgttgca
     8641 ctggagaatg agggtgttga cctcgcggaa cgggtccatg cgggcgctgt cgggatccgg
     8701 catcagaagc atgtcggagg cttcaatacc gcgccagccg ataaaggacg agccgtcaaa
     8761 gggctgtcca agctcgaaca cgtcctcgtc aatgcagtcg gcgggaaccg tcacgtgcat
     8821 ttccttgccg agcgtgtccg tgaggcgaag gtccacgaac ttgacatcgt tgtccttgat
     8881 catctgcaga acatcagaag cggacgtcat ttgatttttc tcctagagat tagccggtaa
     8941 ggcgatccat gcgcctgacc tgtgaggaat tgtctgccga cgggggcgcc cccgacggtc
     9001 gtactccaat ataagcaaaa ggcgtgccaa aacccgaaga ctgcactctc acgcgcgttt
     9061 tcccgattct gacgcctgtg ccgatacctg cgcccagaag gcttcacccc tgcactgcgc
     9121 tgaacgcacc gacttggttg cgaacgcttt attatcgcac caaatcagtg catttcaaca
     9181 cctcaggagc gccttcttcg agtgcgtgcg ggttgcgcca tccgtcagag aaaggcttcc
     9241 tgcagatccg aaagaaaggc ccgccctctg gctgtcggac gaatcaccaa ctcatccgcc
     9301 gccagcagtc cctgcacacg caaatcggaa agtatcggct caacgacctt gagcgaaagc
     9361 ccagtgcgag cctcaaaaag ttccgcaggc acgccttccg tgagcctcag ggcgttgagc
     9421 atgaattcga agggaaggtc cccagactcg acggccctgc gttcggcaat gccgcttccc
     9481 gtttcagaaa ccgaagccat gtacttgcgg ggggatgcgt gccgcacctc gcgaacaact
     9541 ccgtcctgtg tcgtaacctt tccatgcgcg cccgctcccg cagcgagata gtcgccaaag
     9601 gtccagtaat tgaggttgtg gcggcaacgg cacccgggtc cggcatagcc cgacacctcg
     9661 tagtgctcga agcccgcttc ttcgagcgag cgaatcacca gatcagacat gtccgcacag
     9721 aggtcatcgt caggaaggtc ctccgggacg cgcttggcga acgcggtacc gggttcaatc
     9781 gtgagctggt agaacgaaag gtgtgtggca ccggtaacaa ccgccgtctc cacctccctc
     9841 gccacctcct cgagtgtttc accaggcagc gcgaacatca gatcaaggtt gaagtttccg
     9901 aacacctcac ccgcatgcgc ggccgcgcgc agggcctctt ccgatacgtg aatccttccg
     9961 agcttccgaa gcttttcgtt catgaagctc tgcacgccaa cggaaactcg gttgacgcct
    10021 gcatcgcgga aagccctgaa gcggccctcg tccactgtgc cggggttggc ctctagcgta
    10081 atctcacagt cggatgtgaa cccgaacagt ccgtcggccc cttcaaggag tctggccacg
    10141 gcatcaccac tcatcaggct cggcgtgccg ccgccaatga agaccgtttc gattcgacgg
    10201 ccgcctgcga cggaaagcca gccctccaga tccttcaata cgatgtcgac atattccctt
    10261 tctgccaaat cggcaggcgc ggcatgggag ttgaagtcac agtagggaca cttgcgcacg
    10321 caccagggcc agtgcagata aagcgacagc ggaatattcg caaccgcttc agccgtccac
    10381 gaagattgag cgctcatcac cagccccagg cctcgcgaag cagcttgtgc atctgcacaa
    10441 gggccttgcc gcgatggctc acgcggttct tttcctctgc ggtgagctct gcggccgttc
    10501 gaccgtactc aggtacaaaa aagtgaggat cgtagccgaa gccgccctca cccgcaggct
    10561 cgtcgcagat ttcgccgtac cagcggccga cggccacgag cggctcggga tcgtcgggcg
    10621 tgcgcacggc cgtcagcacg caggtgtagt gcgccctcct gtcgtccttg ccctgcagtc
    10681 tctcgaccag aagacggttg ttggcctcat cattgctcgg ctctcccgca aagcgggccg
    10741 acaaaacgcc gggcatgccg ttcaaggcat tgacgcaaac gcccgaatcg tccgccatgg
    10801 cggggcgtcc cgtcatcttc gacgcatgac gagccttggc gagacaattc tcaatgaagg
    10861 tgccgaaggg ctccggacag gcttcgacac caagcgcgcc ctgattgatc acatcgacgc
    10921 cgaactcggc gaacatcgcc tgaaactcac gaagt
//
LOCUS       DBMDTI010000044        10944 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1781_length_10944_cov_2.816010, whole genome shotgun sequence.
ACCESSION   DBMDTI010000044 DBMDTI010000000
VERSION     DBMDTI010000044.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10944)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1781_length_10944_cov_2.816010"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="argG"
                     /locus_tag="ACZPB6_03695"
     CDS             complement(<1..663)
                     /gene="argG"
                     /locus_tag="ACZPB6_03695"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR1183611.1"
                     /translation="MSETILQSVPVGQKVGIAFSGGLDTSAALRWMKNKGALPYAYTA
                     NLGQPDETDYEAIPRRAMEYGAENARLIDCREQLVNEGIAAIQSGAFHISTAGQLYFN
                     TTPLGRAVTGTMLVAAMREDGVNVWGDGSTYKGNDIERFYRYGLLANPNLKIYKPWLD
                     VQFISELGGRAEMSAFLQKEGFNYRMSAEKAYSTDSNMLGATHEAKDLESLDSNMKIV
                     EPI"
     gene            1278..2321
                     /gene="ansB"
                     /locus_tag="ACZPB6_03700"
     CDS             1278..2321
                     /gene="ansB"
                     /locus_tag="ACZPB6_03700"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HIR1183612.1"
                     /translation="MSMKQTLIGLGLVAASGAALALPNIAVLATGGTIAGAGASATGT
                     AYQAGKVSVNHLVAAVPELANIANVTPKQVAQIGSQDMSDEVWLKLAQTINADCKKYD
                     GFVITHGTDTMEETAYFLNLTLKCDKPVAMVGAMLPSTGLGADGPRNLYNAVLTAATP
                     ETAKQGVVVAMDNIVVGAREVIKTNTVQPETFQAANFGKVGTIFNNKVTYSTLPVRAH
                     TTATPFDVTGLKELPKVGIVYNHAGVEGLQAEALVKAGYKGIVNAGVGNGNIHKSIWP
                     VLEQATKNGAVVVRSSRVPTGATTKDAEVDDKAQHWVSSGELNPQKARVLLQLALTKT
                     SDWKEVQKYFDLY"
     gene            complement(2533..2608)
                     /locus_tag="ACZPB6_03705"
     tRNA            complement(2533..2608)
                     /locus_tag="ACZPB6_03705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2572..2574),aa:Arg,seq:cct)
     gene            complement(2696..5149)
                     /locus_tag="ACZPB6_03710"
     CDS             complement(2696..5149)
                     /locus_tag="ACZPB6_03710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1183613.1"
                     /translation="MSSDLLQSLNPRQLQAVTAPEKSVLILAGAGSGKTRVLTTRIAW
                     LLSEHKAQTGDILAVTFTNKAAKEMLVRLEAILPYDLRRMWVGTFHGLCNRILRTHAQ
                     DAGLPKTFQILDSSDQLSMVKRVMKALNIDTESYDPRQVANVINWYKENGMRASHAAG
                     QQGGDETAVKVYQAYEQQCQREGVVDFAELLLRCYELLDRNEIVRSHYQNRFRHILVD
                     EFQDTNVLQYRWLQMLAGFGRGPGGAPMNAVFAVGDDDQSIYAFRGANVGNMSDFLRD
                     FGVEEPIRLEENYRSTGTILDAANALIAHNSNRLGKNLWTSGSRGDRICVAELDDDRA
                     EAEWIAREIEADRSRGRAWRDHAILYRANAQSRALESAFTARGIPYRIYGGQRFFERA
                     EVKHVLAYLRLMDNPGDDTSFQRVVNFPLRGIGARTIETLAEEAAARGLTLFGTLFAP
                     DYKVPPKLAAFRELIMNMREEADSLSLQDAIKLVIVRSGLEAHYNAEKDGEDRIANMR
                     EMMSAAAGYIANEGLPDSMPAFRPADEGGQTPLQGFLTQATLEAGDKNEQGDVDAVQL
                     MTVHAAKGLEFPQVFIAGVEEGLFPHFSALKDERGPGVSEERRLMYVAITRAKQRLVI
                     TYCKSRMQYGEYRRNEPSSFLDEIPEQLVDNKDLTAEAQGYRSSYGGQSRASGYGWER
                     PSFGGSRGGERSYGRSSSSSRSWSSSSSSRGGSSSYGSSRSSASQDKDDWRRGLSGAG
                     GTYRSGEDPVVKKAAARAAGSTFGYEAGDKVEHKKFGRGVIESITGSGNDARVYIRFN
                     DVGRKELLLALAAKNMQKK"
     gene            5380..8016
                     /gene="leuS"
                     /locus_tag="ACZPB6_03715"
     CDS             5380..8016
                     /gene="leuS"
                     /locus_tag="ACZPB6_03715"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR1183614.1"
                     /translation="MRETYSAQAVESEVQAMWKAKDVYRAVENAVDNNGQPKPKFYVC
                     SMLPYPSGKLHMGHVRNYTLNDVMYRFYRMKGYNVMTPMGWDAFGLPAENAAIAKKVA
                     PAAWTYSNIADMKSQMEPLGLAFDWSREVATCKPEYYRWSQWMFLKMLEKGIAYRKTQ
                     IVNWDPVDKTVLANEQVIEGRGWRSGAVVEKREIPGYYLGITQYAQELLDDLDQLQGW
                     PEQVRRMQEHWIGRSEGVNLSFPYELEGTPKLLRVYTTRPDTLMGVSFVAIAAEHPLA
                     AYVSRNRPDLQAFIEECSKGSVSEADMASMEKKGVPTGFYVRHPITGDNVEVWIANYV
                     LMSYGEGAVMGVPAHDERDFAFAKKFGLPIRQVIAVEGETYSTEAWQDWYGDKTLDGR
                     LVNSGKFDGLGIHEAFDAIATDLKARGLGDKQVQFRLRDWGISRQRYWGTPIPMINCP
                     CCGPVPVPEQDLPVVLPEDLIPDGSGNPLTKCESFLNVKCPKCGGDAQRETDTMDTFV
                     DSSWYFQRYCSPDCSTAMVDKRADYWMPMDQYIGGIEHAVLHLLYARFWTKVMRDLGL
                     VKYDEPFKNLFTQGMLMADCYYRNGDDGRKVWFYPDEVEVQYDEKGRPFGAVCKADGQ
                     PVTLGGIEKMSKSKNNVVEPRDIIKKFGADTARSFVMFAGPPDQSAAWSNSGAEGTYR
                     FLRRIWNWAFAKKNLIASAGALEGQMLPHDAKTLRREVHTTLRQAEYDFSRMQYNTVV
                     SACMKMFNTIEGFKGETPADLAALCECASILLRTLYPIAPHITTALWQELGFVERIGD
                     LIDAPWPEVSEDAIKADELKLVVQVNGKLRGEILVPAEAAQAEIEAAALANPDVQRFT
                     EGMTVRKIIIVKNKLVNVVVAK"
     gene            8205..8717
                     /locus_tag="ACZPB6_03720"
     CDS             8205..8717
                     /locus_tag="ACZPB6_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly lipoprotein LptE"
                     /protein_id="HIR1183615.1"
                     /translation="MTSRRHLLGAALIGALPLTGCGFRLRGSYSTPFRTLYLQMPENS
                     YLAVRLRQELLAGSDVTIVNTPSEAEAILELLSDSRSRDVLSINDTGRAREYEITLSL
                     SFRVSNPSGIDYMEAVTLTASRDLTYSESDFLSREKEEAFLYRDMEADLVNQLMRRIE
                     AIKPPKQAGG"
     gene            8721..9722
                     /gene="holA"
                     /locus_tag="ACZPB6_03725"
     CDS             8721..9722
                     /gene="holA"
                     /locus_tag="ACZPB6_03725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR1183616.1"
                     /translation="MKADQLQGYLARSAKDGRLDPFWLLTGSDAFLAVEAGDAIRAEA
                     RRQGYTDRQVLDMNASADWSRLPMAAADIGMFDDRKIVDVRLSTGNPGKNGAPAIVKY
                     LDNPIDGVVTLITMPAPDWTVTKEAWWRALQRKATVVDCSPVERAQLPSWLKARLARH
                     ELVMSPETLDYFADMMEGNLFAAAQEIEKLSLLHPKGEITSEQLRQAVSSNARFDFDT
                     LFESMRLGQADRIVRIIDGLEAQAEALPFLLAMLTAEIRSLIKLRTGYDNGQSRVTGV
                     FATPSLQKAARRLTVKKLCGALSVCADIDRLVKGLPVPKRDSDPWIELKSVVLFLAS"
     gene            9884..>10944
                     /locus_tag="ACZPB6_03730"
     CDS             9884..>10944
                     /locus_tag="ACZPB6_03730"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR1183617.1"
                     /translation="MPEITITPSDYISRIGRQAREASRSMARASTAAKNAVLERIAEL
                     LLEQEADIRAANEADLEAARAEGLDEVFLDRLTISAVGLRAMAEGCRQIAALEDPVGT
                     VTDLAFRPSGIQVGRMRVPLGVLAMIYESRPNVTIDAAALAVKSGNAIILRGGHEALR
                     SNTVLWELVGRALAEKGLPARAVQLIESSDRALVGELIRARDYVDVLIPRGGKSLVAR
                     LTAEATVPMIKHLEGICHTYVDAEADLDIAVRVTENAKTQRYSPCNATETLLVHRAVA
                     FDFLPPMARVFHDHDVEMRCDELSRRIVEQSGMSAVDATPQDWDTEYNAPIISIRIVE
                     SLDEAIDFINLHSSHHTDA"
BASE COUNT         2127 a         3660 c         3144 g         2013 t
ORIGIN      
        1 gatcggctca acgatcttca tgttggagtc gaggctttcc aggtctttgg cctcatgcgt
       61 ggcgccgagc atgttcgagt cggtcgagta ggccttctcg gcgctcatgc ggtagttgaa
      121 gccctccttc tgaaggaagg cagacatttc ggcgcggccg ccgagctcgc tgatgaactg
      181 cacgtcgagc caaggcttgt agatcttgag gttcgggttg gcaagaagac cgtagcggta
      241 gaagcgctcg atgtcgttgc ccttgtacgt ggatccgtca ccccagacgt tgacgccgtc
      301 ctcacgcatg gcggcgacga gcatcgtgcc cgtcacggcg cggccgagcg gagtggtgtt
      361 gaagtaaagc tggcctgccg tcgagatgtg gaaggcgccg gactgaatgg cggcgatgcc
      421 ttcgttcacg agctgttcgc ggcagtcgat caggcgggcg ttttcggcgc cgtactccat
      481 ggcgcgacgc ggaatggctt catagtcagt ttcgtcgggc tggccgaggt tggccgtgta
      541 ggcgtaaggc agagcgccct tgttcttcat ccagcgaaga gcagcgctcg tatcgaggcc
      601 gccggaaaag gcaatgccga ctttctggcc gacgggaacg ctttgaagga tcgtttcaga
      661 catgttttga gtttcttgtg agttcgtgtt gttgtttacc cagcaacctg gggcctgtgg
      721 cgatgattat ccgctctctc agccccgcct gccgcctccg aacgccacta tgtgagactt
      781 tttgaagagt cctcaaacag cttgaggagt tgtacctaga cggtctgaga acaacagaca
      841 aatggtcgac atatgaccgc atttgtccgc ctgcgcaccg cacccatcgc agggaaacac
      901 accaaatccg taagaaccct caggtacatg cttgtttgct cttaaacgac accttccctc
      961 ctttcccctc caggagaaca gttctcgaac aatccgttac aggaaaatca gggaagggct
     1021 tttccatttt tccatagggg ttttcctatt ttttaatctt tgtagtccaa gatcaaacgc
     1081 aatgcactgt tgattgcttg acctcaatca atccgtatag actgaatgag ttgcctgaag
     1141 gtaccgcggt ttcaggacca tgaaaacgaa ctgcagggta cgcctgacgg ccagtccgcc
     1201 acttttcaag cggcggacac tcaaacaaca ggagcgccat tcaactgacg cgctccaaac
     1261 tagaggagca aacaaccatg tccatgaagc agaccctgat cggcctcggc ctcgtcgccg
     1321 cctccggcgc cgccctcgcc cttccgaaca tcgccgttct cgccaccggc ggcacaatcg
     1381 ccggtgccgg cgcctccgct acgggcactg cctatcaggc cggcaaggtg agcgtcaacc
     1441 atctcgttgc cgccgttccg gaacttgcca acatcgccaa cgtgaccccg aagcaggtcg
     1501 cccagatcgg ctctcaggac atgtccgacg aagtctggct caagctcgcc cagacgatca
     1561 acgccgactg caagaagtat gacggcttcg tcatcacgca cggcacggac acgatggaag
     1621 aaaccgccta cttcctgaac ctcacgctca agtgcgacaa gcccgtcgcc atggtcggcg
     1681 ccatgctgcc gtccacgggc ctcggtgctg acggcccgcg caacctctac aacgccgttc
     1741 tgaccgccgc cacgcctgaa accgccaagc agggcgtcgt ggtcgccatg gacaacatcg
     1801 tcgtcggcgc tcgtgaagtc atcaagacca acacggttca gcccgaaacg ttccaggccg
     1861 ccaacttcgg caaggtcggc acgatcttca acaacaaggt gacgtactcc acgctcccgg
     1921 ttcgtgctca cacgaccgcc acgccgttcg acgtcacggg tctgaaggaa ctcccgaagg
     1981 tcggcatcgt ctacaaccac gccggcgtgg aaggcctcca ggctgaagcg ctcgtcaagg
     2041 ccggctacaa gggcatcgtg aacgccggcg tcggcaacgg caacatccac aagtccatct
     2101 ggccggttct tgaacaggcc acgaagaacg gtgccgtcgt tgttcgctcc tcccgcgttc
     2161 cgacgggcgc caccacgaag gacgctgaag tggacgacaa ggcccagcac tgggtctcct
     2221 ccggcgaact gaatccgcag aaggcccgcg tcctcctgca gctcgccctg acgaagacct
     2281 ccgactggaa ggaagtccag aagtacttcg acctctacta atcgaagtct ccgcctgcgc
     2341 ttcacatgat tgctgaagcg catgactcaa gtcttgaaaa agccctgatc cgaaaggttc
     2401 agggcttttt tcatcacagc ggcggcacgg ccgcagccaa ggcagctgtt gaacaaatac
     2461 agggactctc cagaaacggc aatggcccgt tcacattgaa cgagccactt cgttttgacg
     2521 tcaagatgtg cctggtgcgg ccaccgggac tcgaacccag atccgtccct taggagggga
     2581 cagttctatc cagttgaact atggccacca ccgtcctgtc ttgcgtcagg gtagtgaatt
     2641 ctagcagatc ctgaccgatt gcagtgaagt ccttgcaaaa agccagtccc gtgcatcact
     2701 tcttctgcat gttcttcgct gcaagcgcaa ggaggagctc cttgcggcca acgtcgttga
     2761 agcggatgta gacgcgggca tcattgcccg agcccgtgat cgactcaatc acgccgcgac
     2821 cgaacttctt gtgctctacc ttgtcgcccg cctcgtagcc gaatgtagag cctgctgcgc
     2881 gcgcggccgc cttcttcacg acgggatcct cacccgagcg gtaagtgccg cctgcgcccg
     2941 agagtcccct gcgccagtcg tccttgtcct gagaagcgga cgagcgcgag gaaccgtagg
     3001 aggacgagcc accgcgggac gacgaagacg aactccagct gcgggaggaa gaggacgagc
     3061 ggccgtagct tcgctcgcca ccgcgcgatc cgccgaagga cggacgctcc catccgtaac
     3121 cacttgcacg actctgcccg ccgtacgaac tcctgtagcc ctgtgcttcg gccgtcaggt
     3181 ccttgttgtc gaccagctgc tcaggaatct cgtcaaggaa gcttgagggt tcgttcctgc
     3241 ggtattcgcc gtactgcatg cggctcttgc aatacgtgat gaccagacgc tgcttggcgc
     3301 gcgtgatggc cacgtacatc aggcggcgtt cttcggagac gcctggaccc cgctcgtcct
     3361 tgagggcgga gaagtgcggg aagagtcctt cttcgacacc ggcgatgaag acctgcggga
     3421 actccagacc cttggcggcg tgcaccgtca tgagctgaac ggcgtccaca tcaccctgct
     3481 cgttcttgtc acccgcctcg agcgtcgcct gagtcagaaa gccctgaagc ggcgtctgac
     3541 cgccttcatc ggcgggcctg aaggcaggca tgctgtcggg cagaccctcg ttggcgatgt
     3601 agcccgccgc agccgacatc atttcacgca tgttcgcaat gcggtcctcg ccgtccttct
     3661 cggcgttgta gtgggcctca agacccgagc gcacgatgac gagcttgatg gcgtcctgaa
     3721 ggctgaggga atccgcttcc tcgcgcatgt tcatgatgag ctcgcggaag gccgcgagct
     3781 tcgggggcac cttgtagtcg ggcgcaaaga gtgtgccgaa gagcgtgagt ccgcgtgcgg
     3841 cggcttcctc cgccagcgtc tcgatcgtgc gcgcaccgat cccgcgaagc gggaagttga
     3901 cgacgcgctg aaagctcgtg tcgtcgcccg ggttgtccat caggcgcaga taggcgagca
     3961 cgtgcttgac ttcggcacgc tcgaagaagc gctgtccgcc gtagatgcgg tagggaatcc
     4021 ctcgggcagt aaaggcgctt tcgagcgcgc gcgactgcgc gttcgcacga tagaggatcg
     4081 catggtcgcg ccaggcgcgg cctcgggagc ggtccgcctc gatttcgcgg gcgatccact
     4141 ctgcttcagc gcggtcgtcg tcaagctctg ccacgcagat gcggtcgccg cggctgcccg
     4201 acgtccagag attcttgccg agacggttcg aattgtgtgc aatgagcgcg ttggccgcgt
     4261 caagaatcgt ccccgtcgag cgatagttct cctcaagcct gatgggctcc tcaacgccga
     4321 agtcgcggag gaagtcgctc atgtttccca cgttcgcacc tcggaaggcg tagatcgact
     4381 ggtcgtcgtc acccacggcg aagacggcgt tcatcggtgc gccgccgggg cctcgtccga
     4441 accccgccag catctgaagc caacggtact gcaggacgtt cgtgtcctgg aactcgtcga
     4501 cgagaatgtg acggaagcgg ttctgataat gcgagcggac gatttcattg cggtcgagaa
     4561 gctcgtagca gcgcagaaga agctcggcaa agtccacgac gccttcgcgc tggcactgct
     4621 gctcgtaggc ctgatagacc ttgacggcgg tctcgtcgcc gccctgctgg cctgcggcgt
     4681 gcgacgcacg catgccgttt tccttgtacc agttgatgac gttcgccacc tgacggggat
     4741 cgtagctctc ggtatcgatg ttgagcgcct tcatgacgcg cttgaccatg gagagctgat
     4801 cgctcgagtc aagaatctgg aaggtcttcg ggagccccgc gtcttgggcg tgggtgcgca
     4861 gaatgcggtt gcacaggccg tggaaggttc cgacccacat gcggcgcaga tcgtagggca
     4921 ggatagcctc cagtcggacg agcatctcct tggcggcctt gttcgtgaag gtcacagcca
     4981 gaatgtctcc cgtctgggcc ttgtgctcgc tcaagagcca ggcgatgcgc gtcgtcagca
     5041 cgcgcgtctt gcccgaaccc gcgcccgcca gaatgagtac gctcttttcg ggggccgtga
     5101 cggcctgcag ctggcgggga ttgagggact gaagaagatc ggacgacatg cgggaaaacc
     5161 tccggcgaag aattcggaag ccggcaggaa gggccggacg ggctaaaagg tagcacattt
     5221 cgacgacagg ctcacgcccc cgctccgctc ccctccccaa cgcccgaaat cacgacctgg
     5281 atgccatccg ctaggcagtc cctgcaatgc cgaaggcgct ccgcaacagg tatactaggc
     5341 aattattctt tttttgaacc ctcttctttg gaaggcgtta tgcgcgagac ttactctgct
     5401 caggcagtgg aatccgaagt ccaggccatg tggaaggcca aggacgtcta ccgtgccgtc
     5461 gagaacgcgg tcgacaacaa cggacagccc aaacccaagt tctacgtctg ctccatgctc
     5521 ccttacccga gcggcaagct tcacatggga cacgtccgta actacacgct caacgacgtg
     5581 atgtaccgct tctaccgcat gaaaggctac aacgtgatga ccccgatggg atgggacgcc
     5641 ttcggcctgc cggctgaaaa cgccgccatc gccaagaagg ttgctcccgc cgcctggacg
     5701 tactcgaaca tcgcggacat gaagagccag atggagccgc tcggcctcgc cttcgactgg
     5761 tcgcgcgaag tcgccacttg caagcccgaa tactaccgct ggagccagtg gatgttcctc
     5821 aagatgctcg agaagggcat cgcctaccgc aagacccaga tcgtcaactg ggaccccgtc
     5881 gacaagaccg tgcttgccaa cgagcaggtg attgaaggcc gcggctggcg ctctggtgcc
     5941 gtcgtcgaaa agcgcgagat ccccggctac tacctcggca tcacgcaata tgctcaggaa
     6001 ctccttgacg acctcgacca gcttcagggc tggcccgagc aggtccgccg catgcaggag
     6061 cactggatcg gtcgctccga aggcgtgaac ctctccttcc cgtacgaact cgagggcacc
     6121 ccgaagcttc tgcgcgtcta cacgacccgc cccgacacgc tcatgggcgt gagcttcgtt
     6181 gcgatcgccg ccgagcatcc gctcgccgcc tacgtctccc gcaaccgccc cgaccttcag
     6241 gcctttatcg aggaatgctc gaagggcagc gtgagcgaag ccgacatggc ctccatggaa
     6301 aagaagggcg tgcccaccgg cttctacgtc cgccacccga tcacgggcga caacgtcgag
     6361 gtctggatcg ccaactacgt tctcatgagc tacggtgagg gcgccgtgat gggcgtgccc
     6421 gcccacgacg agcgcgactt cgccttcgcc aaaaagttcg gtcttccgat ccgtcaggtc
     6481 atcgccgtcg agggcgagac ctactccacc gaagcatggc aggactggta cggcgacaag
     6541 acgcttgacg gccgtctcgt caactccggc aagttcgacg gcctcggtat ccacgaagcc
     6601 ttcgacgcca tcgccaccga cctcaaggcc cgcggcctcg gcgacaaaca ggtccaattc
     6661 cgtctgcgcg actggggcat ctcccgccag cgctactggg gcaccccgat cccgatgatc
     6721 aactgcccgt gctgcggccc cgtgcccgtc cccgagcagg atctccctgt cgtgctgcct
     6781 gaagacctga ttcccgacgg ctccggcaac ccgctcacca agtgcgaatc cttcctcaac
     6841 gtcaagtgcc cgaagtgcgg cggcgacgcc cagcgcgaaa ccgacacgat ggacacattc
     6901 gtcgacagct cctggtactt ccagcgctac tgctcgcccg actgctccac ggccatggtc
     6961 gacaagcgcg ccgactattg gatgccgatg gaccagtaca tcggcggcat cgagcacgcc
     7021 gtgctccatc tgctctacgc ccgcttctgg acgaaggtga tgcgcgatct cggcctcgtc
     7081 aagtacgacg agccgttcaa gaacctcttc acccagggca tgctgatggc cgactgctac
     7141 taccgcaacg gcgacgacgg ccgcaaggtc tggttctacc cggacgaggt tgaggtccag
     7201 tacgacgaga agggccgccc cttcggtgcc gtctgcaagg ccgacggtca gcccgtcacc
     7261 ctcggcggca tcgaaaagat gtccaagtcg aagaacaacg tggtcgagcc gcgcgacatc
     7321 atcaagaaat tcggcgccga cacggcccgc agcttcgtga tgttcgctgg tcctcccgat
     7381 cagtccgccg cctggtccaa ctccggcgcc gaaggcacct accgcttcct gcgccgcatc
     7441 tggaactggg cattcgcgaa gaagaacctc atcgcaagtg caggtgccct tgaaggccag
     7501 atgcttccgc acgacgccaa gaccctgcgc cgcgaagtcc acacgacgct tcgccaggcc
     7561 gagtacgact tcagccgcat gcagtacaac acggtcgtct ccgcctgcat gaagatgttc
     7621 aacacgatcg aaggcttcaa gggcgagacg cccgccgatc tggcggctct ctgcgaatgt
     7681 gcctcgatcc tgctgcgcac gctctacccg atcgccccgc acatcacgac ggcgctctgg
     7741 caggagctcg gcttcgtcga acgcatcggc gacctgatcg acgcaccgtg gcccgaagtc
     7801 agcgaagacg caatcaaggc cgacgagctc aagctcgtcg tccaggtcaa cggcaagctc
     7861 cgcggcgaga tcctcgtacc cgccgaggcc gctcaggccg aaatcgaggc cgccgcactc
     7921 gccaatccag acgtgcagcg cttcactgaa ggcatgacgg tccgcaagat catcatcgtg
     7981 aagaacaagc tcgtgaacgt cgtggtcgcc aagtaaggcg ccgcaccttc aagctgacca
     8041 tcctaagggg gcctgtgtcc gaccggacgc aggccctcgt tctttcgcac tcccccgtct
     8101 tcttgactcc ttctcgactc ctgcggtcgg ccgttataat tgatgattcc gaagcccttc
     8161 caaggggcct ccaacatcat cctgccatcc cgaggatcat ctccgtgact tcccgtcgtc
     8221 atcttctcgg cgccgccctc atcggcgcac tcccgctcac aggctgcggc ttccggctgc
     8281 gcggctccta ttcgacgccg ttcaggaccc tgtacctcca gatgccggaa aactcgtacc
     8341 ttgcggtgcg tctcagacag gagcttctgg caggatccga cgtcacgatc gtgaacacgc
     8401 cctccgaggc cgaagcgatc cttgaactcc tctccgactc ccgttcgcgc gacgtcctct
     8461 cgatcaacga cacgggccgc gcccgtgaat acgagatcac gctctcgctc tccttccgag
     8521 tgtcgaaccc cagcggcatc gactacatgg aagccgtgac gctcaccgcc agccgagacc
     8581 tcacctactc cgaatcggac ttcctctcgc gtgaaaagga ggaggcattc ctctaccgcg
     8641 acatggaggc cgacctcgtc aaccagctca tgcgccgcat cgaggcgatc aagcccccga
     8701 agcaggcggg aggctgatcc gtgaaggccg accagctcca gggctacctc gcgcgatccg
     8761 cgaaggacgg acgtctggat cccttctggc ttctgacggg ctcggacgcc ttcctcgccg
     8821 tcgaagcagg cgacgccatc cgtgccgaag cgcgccgaca gggctacaca gaccgtcagg
     8881 tgctcgacat gaacgcctca gcggactggt cgcgcctgcc gatggccgcc gccgacatcg
     8941 gcatgttcga cgaccgcaag atcgtcgacg tgcgtctctc cacggggaat ccgggcaaga
     9001 acggcgcgcc cgccatcgtg aagtatcttg acaacccgat cgacggcgtc gtcacgctca
     9061 tcacgatgcc cgcccccgac tggacggtca cgaaggaggc gtggtggagg gcgcttcagc
     9121 gcaaggccac cgtcgtagac tgctcgcccg tggaacgcgc acaactcccc tcctggctca
     9181 aggcaagact cgcccgccac gagctcgtca tgtcccccga gacgctcgac tacttcgccg
     9241 atatgatgga gggcaacctc tttgctgccg cacaagagat cgaaaagctc tctcttctcc
     9301 atcctaaggg cgagatcacc tccgaacagc tccgtcaggc cgtctcgagc aacgcccgat
     9361 tcgatttcga cacgctcttt gagagcatgc gcctcggtca ggccgaccgc atcgtgcgca
     9421 tcatcgacgg gcttgaggcg caggccgaag ccctgccctt cctgctggcc atgctcactg
     9481 ccgaaatccg ttccctcatc aagctgcgca cgggctacga caacggccag tccagagtga
     9541 ccggagtctt cgcaacgccg tcccttcaga aggccgcccg ccgcctcacc gtgaagaagc
     9601 tctgcggcgc cctctccgtc tgcgccgaca tcgacaggct cgtcaagggg cttcccgttc
     9661 ccaagcggga ctccgatccc tggatcgagc tcaagagcgt cgtcctcttt ctcgcctcct
     9721 gaagccggct ccacgggagg aggccttcgc gcatccgcag gtcctgcaaa gatcaaagag
     9781 atgccgtcgt tcgggctaaa atggaaagac atgcccggaa aatgcatgcc gccccgtcct
     9841 ttgagggcgg cgttctccgg ccctttttca ggaaccgcgc atcatgcccg aaatcaccat
     9901 cactcccagc gattacatca gccgcatcgg ccgtcaggcc cgcgaagcct cgcgctccat
     9961 ggctcgcgcc tccacggccg ccaagaatgc ggtgcttgaa cgcatcgccg agcttctgct
    10021 cgaacaggag gcggacatcc gcgccgccaa tgaggccgac ctcgaggccg cccgcgccga
    10081 aggtctggac gaggtctttc tcgatcgtct gacgatcagc gccgtaggtc ttcgcgccat
    10141 ggccgaagga tgccgccaga tcgccgcact cgaggatccc gtgggtactg tcaccgatct
    10201 tgccttccgg cctagcggca ttcaggtggg ccgcatgcgc gtgcccctcg gcgtgctcgc
    10261 catgatctac gagtcccgtc ccaatgtgac gatcgacgcc gccgcccttg ccgtcaagtc
    10321 gggcaacgcc atcatcctgc gcggcggcca tgaggcgctt cgcagcaaca cggtcctctg
    10381 ggagcttgtc ggccgggccc ttgccgaaaa aggcctcccc gcacgcgccg tccaactcat
    10441 cgaatcctcc gaccgcgccc tggtgggcga gctcatccgt gcccgcgact acgtcgacgt
    10501 gcttattccg cgcggcggca agagcctcgt cgcccgtctc acggccgaag cgaccgttcc
    10561 gatgatcaag catcttgagg gcatctgcca cacctacgtc gacgccgaag ccgacctcga
    10621 catagccgtt cgcgtgactg aaaacgccaa gacccagcgc tactcgccct gtaacgccac
    10681 tgaaacgctg ctcgtgcacc gcgccgtcgc cttcgacttt ctgcctccca tggcccgcgt
    10741 cttccacgac cacgatgtcg agatgcgctg tgacgagctc tcccgccgca tcgtcgaaca
    10801 gagcggcatg agcgccgtcg acgccacccc gcaggactgg gataccgagt acaatgcccc
    10861 gatcatttcg atccgcattg tcgaaagtct cgacgaggcg attgacttca tcaacctcca
    10921 ttcctcgcac cacaccgacg ccat
//
LOCUS       DBMDTI010000045        10613 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1830_length_10613_cov_2.628520, whole genome shotgun sequence.
ACCESSION   DBMDTI010000045 DBMDTI010000000
VERSION     DBMDTI010000045.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10613)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1830_length_10613_cov_2.628520"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            334..1719
                     /gene="cuyB"
                     /locus_tag="ACZPB6_03735"
     CDS             334..1719
                     /gene="cuyB"
                     /locus_tag="ACZPB6_03735"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate racemase"
                     /protein_id="HIR1183618.1"
                     /translation="MTKILKIGLVAGVNPIVAPFVKAAIFRAAAESARTVELVEDELE
                     LRKDAPTERIALGDSKITALESAWRLQEKGCDVALIANLRSEAYIDEVRTELQMPIVS
                     LYAGMPEAVKAKGARKIGLLGCALDVALIRKLLPECEIIEGSGLSCTNTTNPEIVREE
                     AVKLLAAGAELLLPVCGRVAGAAFELISEGMPILDVLTTAAHKAIDETPVRLPKPFKV
                     GLIGGLGPAATVDLYDKIVKATPAKNDQEHFKLVVEQNPQISDRTACLLKGGADPTLA
                     MYGCARRLQKDGCDAIIVPCNTAHAFVPYLERHLDIPFINMQIATMEEIKAKLGDKAR
                     IGLLATSGTVQTGIYGDKAKALNLPMFVPDELHQERVMAAIYGPLGAKAGYTDGQCRE
                     DLVSAAEYLVKTYDCNCLILGCTELPLILDECDDFVCAGKEVCVVDPTSALARKVVRI
                     ATETNKARGTR"
     gene            1850..2482
                     /locus_tag="ACZPB6_03740"
     CDS             1850..2482
                     /locus_tag="ACZPB6_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR1183619.1"
                     /translation="MKDLSVYVLVSFIACVTPGSGVLYTVTNGLRGGLRTVLASPLGT
                     TLGVGLMSLISATGLGALITSSPALYAGLQLVSAAVLFWLGWGSWNAGAVRLDKLGRL
                     ESNDSLTFSSIFKGALYVQASNVMLIVFLLSLMPQFISPEDDYVSRVTLLSVLFVGIC
                     FLVHLGYSAIAAVARKWLAGPNFSIWLNRVSGALFWLLGLSVIWNALFNR"
     gene            complement(2485..3297)
                     /locus_tag="ACZPB6_03745"
     CDS             complement(2485..3297)
                     /locus_tag="ACZPB6_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1183620.1"
                     /translation="MTSVTIVVDNFCQRQGLYAEWGFSAFIRTPSGNLLWDTGGILHV
                     LLHNLRALGIRPEGVENLALSHGHFDHASGLTDILRVAPSAKLWASREIARLRWGDAD
                     ASRASGGGPLFAGLPFTPVDGGVEILPEVIAFTVPADERDPRFLVFDNMFETGATGDK
                     VPDTFADDVSLLVKGSAGWSVVLGCAHAGLPNILNYAMKRFGIEGFDAVTGGTHLSGA
                     CSEELPVWMDALAAVPVRRWRVNHCTGFRAAAALAGRFDDVDWAGAGAVLTF"
     gene            3319..3675
                     /locus_tag="ACZPB6_03750"
     CDS             3319..3675
                     /locus_tag="ACZPB6_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183621.1"
                     /translation="MKAGGDLVNRGPNARSSLHYTPEDLENSTPTPPDPPFRALRRLL
                     TLLAALLAGVAAGLIVPVLAGFGERGLAVGIATTALILLGVGIRDRVRDPDGRAGSLF
                     IGAGIVCVFLLIVVLV"
     gene            3748..4086
                     /locus_tag="ACZPB6_03755"
     CDS             3748..4086
                     /locus_tag="ACZPB6_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183622.1"
                     /translation="MKRLLAAAALAAALSCSAAGTQDIQKHGEEYFRIFSILPDGQGI
                     LARRCTKLTQSGLCVGAPVLLPADAVKAPAEEKVVRLADPKEDGWHTLQHDNGQKKTV
                     MKMTGREVTE"
     gene            5011..5736
                     /locus_tag="ACZPB6_03760"
     CDS             5011..5736
                     /locus_tag="ACZPB6_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase domain-containing protein"
                     /protein_id="HIR1183623.1"
                     /translation="MTTTTCSWRPYRVALRTQECRGVISLYLEPTDGALPPAFAAGQY
                     VELRMPGIEPHAFFLVGPSGDRLVRLTIEVGDEPFIGEVGRIAKLRIGDPVELSEPKG
                     ELAMEPGETPVVIMTEDIGCLVAHAFLAEMAEVEPMRNVRVLCQFPNGTRMLMREELR
                     KLVDLMPNAGLAVFLAAPFSTDTAGKDYEVAGPITVENARSVCVNPDADFFVVGSRAF
                     TEALRTDLVKLGIIHSRVHTQVL"
     gene            5932..6462
                     /locus_tag="ACZPB6_03765"
     CDS             5932..6462
                     /locus_tag="ACZPB6_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1183624.1"
                     /translation="MHLTRFTDLSLRVLIYLTHNERKGLVTVTELAERFKWSRNHMVK
                     VVHFMSTKNWVQTHRGRFGGLTLKMHPSEYKIGDIVQILEEQGELIDCFEPHECALAK
                     CCKLRSLLNEATRDFYSRLNTATLETLTNSDEIREIISGKDLLRAQPDAPTPPSSELI
                     RLMEARVARELRKARQ"
     gene            complement(6555..6905)
                     /locus_tag="ACZPB6_03770"
     CDS             complement(6555..6905)
                     /locus_tag="ACZPB6_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1183625.1"
                     /translation="MRAITEERAGTDMVFRVSVPANKADAVRRHLESMMMLLGESVVT
                     IGAPEETPDLPPGPMLKKLRKERGLSQKSLAEQLGTNQVRVSDMEQGVRPITPEFATK
                     LAAVFGVSARFFLV"
     gene            7049..7894
                     /locus_tag="ACZPB6_03775"
     CDS             7049..7894
                     /locus_tag="ACZPB6_03775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1183626.1"
                     /translation="MSKKSLQDLRREVSGFTDEIRRELKEHVSEIRENLDRQVTRLRA
                     EVEDVKEELERRFGTDDSHVVFRALPKPRRLNMPLTEVLERRQTRRTFSDEPLSDLDM
                     ATILWAADGINRSSGRRTTPTALDWRETDIYLLKSNGIWRWVPEKNGLLFCELADLRS
                     ETFFAAPHLKVAPVHIVYVSNRPRTETLISRLGEGVVEKLRHSAWTPEKLEEMRTRSM
                     IIDVGAKIQAVYMAAAAMDLSCVARTGFDAHHVERILRLQKGESVVAIQTLGYRPNSI
                     LDAIK"
     gene            complement(7902..8855)
                     /locus_tag="ACZPB6_03780"
     CDS             complement(7902..8855)
                     /locus_tag="ACZPB6_03780"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1183627.1"
                     /translation="MIKGEKNNIDLSFEDMQFLLALYETQSLTQTAGDMRLSMGAASR
                     RLSHVREVFGDELFVRSGLSMLPTSRMRELRDRLAELVDQTRRLLNPEPLELHEMNRV
                     VRFISADNGIATLLSAAVERFYNAVPNSCFAIEPLDVRVLERLRANEADMAFFPATEL
                     PPDFHSLRIYRSKKGILVRDGHPVIEAYEKAGVMTWELLNRWPAVCVTAGMKDGGAKS
                     VEGRRVGVVLPYFLTVPYVVARTDFTFEGPLITLRRFMMDSQFQFRILPMPEESGPFE
                     PRLVWHHCSHTDPFLQWVRGLVVDAARSEARLLGVIDTSEE"
     gene            9017..10507
                     /locus_tag="ACZPB6_03785"
     CDS             9017..10507
                     /locus_tag="ACZPB6_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1183628.1"
                     /translation="MAGAQAPATIRPTDQTAERRTDQKENQVMWDRLLRNARVVDPVN
                     GRDGVMDVAVKDGRIAAVGPNLQGEASEVEDLTGLVVMPGLIDPHLHLGSMFGSAYGT
                     RMAAAAGVTTCLDMAGPVDEILETSKTCGAGINVAMLEGFSPMKHCGTMTPTREQLEK
                     FVRESLEKGAVGVKIMGGHWPLPLETSRELVKTANDMNAYVAWHAGSHTAGSNILGMR
                     EVIEAAKGQRLHLAHINAYCRGRVNPVDEESKEAIEMLRANPNLWCEAYVSPNNGTVL
                     DCDEDGQIIDHVTRTCLETFGLTPDAAGMREAFLTHRCFAIADTGFMSELVEGEAALE
                     LWEKQGMKGAGSFPVNPALSRFMVATAKREDGSFVVDAISTDGGCIPRNVAISVGLSL
                     VKFGALTLPEFVVKTSVNPARHLRLHDRGHLSEGAAADITVFDYDRQQAVETIVAGKS
                     VMKNGEITGTGITLVTTKAGIPAAERNGFKWIETEFESPEPERYIP"
BASE COUNT         2172 a         3687 c         2869 g         1885 t
ORIGIN      
        1 tgatttttaa agaacagcgc tcctaagagc acagagtcga gagtttacca actcatcaac
       61 tctttgtcaa atttattcac tgatgacttg gcgtcgtcag cgaaggattg aaattaaaca
      121 cgacttcggc acacttgtca aacattcgca tcaaattccg tattaatact tagaaaacgc
      181 acaaaaacca cctttctgca gatgcgactc atgggaaaca ctcttggcaa gaccgtcttc
      241 aaggcgtaat gtgttgggat gaccaagaac cctgtgccgc cccatccctt tcattccatg
      301 cggggcgcac gcatctggtt agaggagaaa cacatgacca agatcctgaa gatcggcctc
      361 gtggccggcg tcaaccccat cgtcgccccg ttcgtcaagg ccgccatctt ccgcgccgca
      421 gccgaatctg cccgcaccgt cgagctcgtc gaggacgaac ttgagctgcg caaggacgct
      481 cctacggagc gcatcgccct cggtgacagc aagatcaccg ctcttgaaag cgcctggcgc
      541 cttcaggaaa agggctgcga cgtggccctc atcgccaacc ttcgctctga agcctacatc
      601 gacgaagtcc gcacggagct tcagatgccg atcgtctcgc tctacgccgg catgcccgag
      661 gccgtgaagg ccaagggcgc acgaaagatc gggctcctcg gctgcgcgct cgacgtcgcc
      721 ctcatccgca agctgctccc cgaatgcgaa atcatcgaag gttcgggcct ctcctgcacc
      781 aacacgacca atcctgaaat cgtccgggaa gaagcagtca agcttctcgc cgccggcgcc
      841 gagcttctgc tccccgtctg cggtcgcgtc gccggcgccg ccttcgagct catctccgaa
      901 ggcatgccca ttctcgacgt cctgaccacc gccgcccaca aggcgatcga cgaaaccccg
      961 gtccgcctgc ccaagccctt caaggtcggc ctcatcggcg gcctcggccc tgccgcaacc
     1021 gtcgacctct acgacaagat cgtgaaagcc acgcccgcca agaacgacca ggaacacttc
     1081 aagctcgtcg tcgagcagaa tccgcagatc tccgaccgca ccgcctgcct tctcaagggc
     1141 ggcgccgatc ccacgctcgc catgtacggc tgcgcccgcc gcctccagaa ggacggatgc
     1201 gatgcgatca tcgtgccctg caacacggcc cacgccttcg tgccctatct tgaacgccac
     1261 ctcgacattc cgttcatcaa catgcagatc gccacgatgg aggaaatcaa ggccaagctc
     1321 ggcgacaagg cccgcatcgg tctgctcgcc acctccggca cggtccagac cggcatctac
     1381 ggcgacaagg ccaaggccct caacctcccg atgttcgtgc ccgatgaact gcatcaggaa
     1441 cgcgtgatgg ccgccatcta cggtccgctc ggcgccaagg ccggctacac cgacggccag
     1501 tgccgcgaag acctcgtgag cgccgcggaa tacctggtga agacctacga ctgcaactgc
     1561 ctcatcctcg gctgcaccga actgccgctc atccttgacg aatgcgacga cttcgtctgc
     1621 gccggcaagg aggtctgcgt cgtcgaccca acctccgcgc tcgcccgcaa ggtcgttcgc
     1681 atcgccactg agaccaacaa ggcccgcggc acccgctgat ccctgatctc aggctgtcag
     1741 gtctgatgtt aggcaaggcg gctgtccgaa cctccggacg gccgcctttt tgtcagaatc
     1801 caagtcaaca actcctgccc aaaggcagag gttttcgcgc cgaatttcta tgaaagatct
     1861 gtccgtctac gttctggtga gcttcatcgc ctgcgtcaca cccggctcgg gcgttctcta
     1921 caccgtcacg aacggactgc gcggcgggct ccgcacggtc cttgcatctc cgctcggcac
     1981 cacgctcggc gtcggcctga tgtcgctcat ctccgcgacg ggcctcggcg ccctcatcac
     2041 ctcatcgccc gcactctatg caggcctgca gctcgtttca gccgccgtgc tcttctggct
     2101 cggttggggc agctggaacg caggcgcagt gcgccttgac aagctaggca ggcttgagtc
     2161 caacgactcg ctcaccttca gcagcatctt caagggcgcc ctgtacgttc aggcgagcaa
     2221 cgtgatgctg atcgtcttcc tgctctcgct catgccgcag ttcatctcgc ccgaggatga
     2281 ctacgtctcg cgcgtcacgc tcctctcggt cctcttcgtc ggaatctgct ttctcgtaca
     2341 cctcggttac agcgccattg cggcggtggc ccgcaagtgg cttgcaggcc cgaacttcag
     2401 catctggctc aaccgcgtca gcggcgctct cttctggctt ctcgggctga gcgtcatctg
     2461 gaacgcactc ttcaaccgct agaatcagaa ggtcaggacc gcgcccgctc ccgcccagtc
     2521 gacgtcgtcg aagcgtcctg ccaacgccgc cgctgccctg aaccccgtac agtggttcac
     2581 gcgccagcgc ctgacgggaa cggctgcgag cgcatccatc cagacgggca gttcctccga
     2641 acaggcgcca ctcaagtgcg ttccgcccgt caccgcatca aagccttcga tcccgaatct
     2701 cttcatcgcg tagttcagaa tattgggaag acccgcatga gcacacccga gcacaacaga
     2761 ccaaccagca ctccccttca cgaggaggga gacgtcgtcc gcgaaggtgt cggggacctt
     2821 gtcaccagtg gctccagtct cgaacatgtt gtcgaagaca aggaacctcg ggtcgcgctc
     2881 gtccgcagga acggtgaagg cgatcacttc aggcaggatt tcgactccgc cgtcgacggg
     2941 cgtgaagggg aggcctgcga aaaggggacc gcctccgctc gccctcgacg catccgcatc
     3001 accccatctg aggcgcgcga tctctcgaga cgcccaaagc tttgccgacg gagccacgcg
     3061 gaggatgtcc gtgaggccag acgcatgatc gaagtggccg tgggaaagcg caagattctc
     3121 aaccccctca ggtcgaatgc caagcgcccg aaggttgtga aggagaacat ggagaatgcc
     3181 tcccgtgtcc cagagcagat tgccggacgg cgttctgatg aaggcggaga atccccattc
     3241 ggcataaagc ccctgcctct ggcaaaagtt gtcgacaacg atcgtgacgc tggtcatgat
     3301 gcaacctcct ccagactcgt gaaggcgggc ggggacctcg tcaacagggg tcctaacgca
     3361 cgctcatcac ttcactatac ccccgaggac ctcgaaaact ccacgccgac cccgcccgat
     3421 cctcccttca gggccctcag acgactcctc acgcttcttg ctgcgctgct tgcaggcgtc
     3481 gctgcgggac tgatcgtgcc cgtccttgca ggctttggcg agcgcggcct cgccgtcggc
     3541 atcgcaacca ccgccctcat acttctcggc gtcggcatca gggatcgagt gcgcgatcca
     3601 gacgggcgcg caggctcgct cttcatcggg gcaggaatcg tctgcgtctt tctcctcatc
     3661 gtcgttctcg tatgagcccg tcaggtccgc ataacaaagc gatcctatat tgagagtctc
     3721 aacggaccat ccacatcagg agaaactatg aaacgtcttc tcgctgccgc agccctcgcc
     3781 gcggcgcttt cctgcagcgc cgcaggcacg caggacatcc agaagcacgg cgaagaatac
     3841 ttccgcatct tcagcatcct gcccgacggc cagggcattc tcgcccgccg ctgcaccaag
     3901 ctcacgcaga gcggcctgtg cgtcggcgca cccgtgttgc tccccgccga cgccgtcaag
     3961 gcgcccgccg aggagaaggt cgtgcgcctc gccgatccga aggaggacgg ctggcacacg
     4021 ctccagcatg acaacgggca gaagaagacc gtcatgaaga tgacgggccg cgaagtcact
     4081 gaatgagcgg ttgagcaagg acatcctcaa gtgggctgca tgcacaagca tgcggcccgt
     4141 ttttcttgcc gttccccgca tccaaacaca aaaacgcccc gaaacatgag gagcagtttc
     4201 agggcgtcaa tgcaaaagca aacggtctgc cgaaccacca gcagaccgtt gccatccaac
     4261 ctatgaagct tcagaggacc agtccgaagc ctccgtactc aagcctgacc aaggctcttg
     4321 tacaagcaca agcaacaggg gaccagcctg cctgcctaag cagacgcttg cgcaaatgtt
     4381 tcttgtccgg gggacttgaa actcgaagcg tcgtcgggag gagagagtat ccgacgatct
     4441 tcttttcttc gacgcagcgg ccaaaggaga aaatgccgtc gcatcgcgtg agcttggtaa
     4501 acccttaagg gttaatccct gctcaacaca gaacgaagta tatacggatt taccccataa
     4561 ggaaacccct aatggcaatt ttttcgccga tacccacctc caagatgaaa caaaatgttt
     4621 tcacctcacc caaaacgccc cctgaatacg gccatttccc ttgtcccaca gccaaccaaa
     4681 cccaaagaac cccattttgc ggaacacagg gttcaatccg cccgaacggc ctctcacagt
     4741 gcgagttcgg ccgttttgtc ccccataggt gccattgacg gaaaagggct ccgccgggcg
     4801 agcggctcag ccgctctcgt ccgttcctgt ttcgtggcct cagggaaaca ccgagcaaaa
     4861 aatgcttctg tttttactgc cgtccccctt aaaattgaat aaagggtgga agttctttcc
     4921 gccccagtca agggatgccg gcagagtcct cccgcgtcga gcaggagcgg aatccggatg
     4981 ccgccgtctc cgaacatggg aggtatgcat atgacaacaa cgacatgcag ctggaggccc
     5041 taccgtgtcg ccctgcgcac ccaggagtgc cgaggcgtca tcagtctcta cctcgaaccg
     5101 acggacggag cgcttccgcc cgcatttgcc gccggccagt acgtcgagct ccgcatgccc
     5161 ggcatcgagc cgcacgcctt cttcctcgtc ggtcccagcg gcgaccgcct cgtgcgcctc
     5221 acgatcgagg taggtgacga gcccttcatc ggcgaagtcg gccgcatcgc caagctccgc
     5281 atcggcgacc ccgtcgaact ctccgagccc aagggcgagc ttgcgatgga gcccggtgaa
     5341 acccccgtcg tcatcatgac cgaggacatc ggctgcctcg tcgcccacgc attcctcgcc
     5401 gaaatggccg aggtcgagcc catgcgcaac gtccgtgtcc tctgccagtt ccccaacggc
     5461 acccgcatgc tcatgcgcga ggagctcagg aagctcgtcg acctgatgcc caacgcgggg
     5521 ctcgccgtct tcctcgcagc ccccttctcg acagacacgg cgggaaagga ctacgaggtc
     5581 gcaggcccca tcactgttga aaacgcaagg agcgtctgcg tcaacccgga cgcggacttc
     5641 ttcgtcgtcg gcagcagggc cttcaccgaa gccctcagga ccgacctcgt caagctcggc
     5701 atcattcact cccgagttca cacgcaggtc ctctgagcgc ttttacccgg gacgggggtc
     5761 gtcgttcgca aacctcccct gcccctgtaa aattcgtcct tgccgatagc gtgatattcc
     5821 aaaacggtct acacttctat ctatctgtct gtcaggcaac ataatttata aaacaacaac
     5881 gtcagacgga ttccatccgt ccgacatccc ccatttaaaa agattccgcc gatgcattta
     5941 actcgattta cagacctcag cctccgcgtg ctgatttacc tcacccacaa cgaacgcaag
     6001 ggactcgtta cagttacaga gcttgccgaa cgcttcaagt ggtcccgcaa ccacatggtc
     6061 aaggttgttc acttcatgag caccaagaac tgggttcaga ctcaccgcgg cagattcggc
     6121 ggcctcaccc tcaagatgca cccgtccgaa tacaagatcg gcgacatcgt tcagattctt
     6181 gaggaacagg gcgaactcat cgactgcttc gagccgcacg aatgcgccct tgccaaatgc
     6241 tgcaagctcc gcagccttct caacgaggcc acccgcgact tctactcgcg cctcaacacg
     6301 gccacgcttg agacgctcac gaacagcgac gaaatcaggg aaatcatctc cggcaaggac
     6361 ctgctccgtg cccagcccga cgccccgacg ccgccgagca gcgagctcat ccgccttatg
     6421 gaggcgcgcg tcgcccgcga actccgcaag gccagacagt aatgcttctg cagcactgat
     6481 cgccccaaaa aagccccgcc aggcgcctgc ctgcggggct tttccgttcc gtcgttttcg
     6541 ctttttcctg acgcctacac caggaagaac ctcgccgaaa cgccgaagac ggccgcgagc
     6601 ttcgttgcga actcgggcgt aatgggcctc acgccctgct ccatgtcgga gacgcggacc
     6661 tgattcgtgc ccagctgctc ggccagagac ttctgcgaaa gtcccctttc cttcctgagc
     6721 ttcttgagca tcggacccgg cggcaggtcg ggcgtctcct cgggtgcgcc gatcgtaacg
     6781 acggactccc cgagaagcat catcatgctc tcgagatgtc ttctcacggc atccgccttg
     6841 ttcgcaggaa cgctcaccct gaacaccatg tcggtgccag cgcgttcttc cgtaatcgcc
     6901 ctcatcagcc agccctcctc atctcaatca acacattcat cacatttggc aacttacgga
     6961 ccgcccagaa cggtccgtct tcgcgctatt ctacagacaa tgcggcaagc gccccgctcc
     7021 gggagcgcac gcatgaagga aagcacgaat gtccaagaaa tcacttcaag acctgcgcag
     7081 ggaggtctcc ggcttcaccg acgaaatccg tcgcgaactc aaggagcacg tatccgagat
     7141 ccgcgagaac ctcgatcgac aggtcacgcg cctgcgcgca gaggtcgagg acgtgaagga
     7201 ggagctcgag cgccgcttcg gcaccgacga ctcgcacgtc gtattccgcg cgctccccaa
     7261 gccccgtcgc ctcaacatgc cgctcacgga ggtcctcgag cgccgccaga cccgccgcac
     7321 cttcagcgac gagcccctgt ccgacctcga catggccacg atcctctggg cggcggacgg
     7381 catcaaccgc tcgagcggac gccgcaccac gcccaccgcg ctcgactggc gcgagaccga
     7441 catctatctt ctcaagagca acggcatctg gcgttgggtg cccgaaaaaa acggccttct
     7501 cttctgcgag cttgcggacc tcaggagcga gaccttcttc gccgcgccgc acctcaaggt
     7561 tgcgcccgtg cacatcgtct acgtctccaa ccgcccgcgc accgagacgc tgatttcacg
     7621 cctcggcgag ggcgtcgttg aaaaactcag gcactccgcc tggacacccg aaaagctcga
     7681 ggaaatgcgc acgcgcagca tgatcatcga cgtgggcgcc aagattcagg ccgtctatat
     7741 ggccgcagcc gccatggacc tctcctgcgt cgcccgcacg ggcttcgacg cccaccacgt
     7801 cgagcgcatc ctgcgcctgc agaagggcga gtccgttgtc gccatccaga cgctcggcta
     7861 caggccgaac tccatcctcg acgccatcaa gtagcgcgcc gtcactcttc ggacgtgtcg
     7921 atcacgccca ggagcctcgc ctcgcttctt gcagcatcca ccacgagccc cctcacccac
     7981 tgcaggaagg ggtccgtgtg gctgcagtgg tgccagacga gcctcggctc gaaggggccc
     8041 gactcctcag gcatcggtag aatcctgaac tgaaactgcg agtccatcat gaagcgcctg
     8101 agggtgatga gcggcccctc gaacgtaaag tccgtcctcg ccaccacata gggaacggtc
     8161 aggaagtaag gcagcaccac gcccaccctg cgtccctcga cactcttcgc gccgccgtcc
     8221 ttcatccccg cggtgacaca gacggcgggc caacggttga gcaactccca ggtcatgacg
     8281 cccgccttct cgtaggcctc gatcacgggg tgtccatccc tgacgagaat gccctttttc
     8341 gagcggtaga tcctgagcga gtgaaagtcg ggcggcaatt ccgtcgcagg aaagaatgcc
     8401 atgtcggcct cgttggcgcg aagcctctcg agcaccctca cgtcgagcgg ctcgatcgca
     8461 aagcagctgt tcggaacggc attgtagaag cgctcgaccg ctgcgctcag tagtgtggcg
     8521 atgccgttgt cggctgaaat gaagcgcacc acgcggttca tctcgtgcaa ctcaagcggc
     8581 tcgggattga gaagtcgacg cgtctggtcc acgagctcgg caaggcggtc cctgagctcg
     8641 cgcatgcggc tcgtcggcag catcgacaaa cccgagcgca cgaagagctc gtcgccgaag
     8701 acctccctca catgcgaaag cctgcgcgag gccgcgccca tgctgagcct catgtcgccc
     8761 gccgtctgcg tgaggctctg cgtttcatag agcgccaaca gaaactgcat gtcctcgaat
     8821 gagaggtcga tgttgttctt ttcgcctttg atcatggtct ttgtcgtcct tagcccaaag
     8881 cggccggcac ggggcaccgc aaccgctcgg aagtgcctag aagtatcgca tttttgccgg
     8941 tggggacgcc gccgagcccc gcgcatatgc gaaaacccta cggaccggtc ggtaaattca
     9001 atcctacaat tgaagaatgg ctggcgcgca ggcgcccgcc accatccgac caacagacca
     9061 gacggccgag cgccgcaccg accaaaagga gaatcaagtt atgtgggatc gtcttctcag
     9121 gaatgcccgt gtcgtggacc ccgtcaacgg ccgcgacggc gtcatggacg tggccgtcaa
     9181 ggacggccgc attgcagccg tcggaccgaa cctccagggc gaagcttccg aagtcgagga
     9241 cctcacgggc ctcgtcgtca tgcccggcct catcgatccc catctgcatc tcggcagcat
     9301 gttcggctct gcttacggca cgcgcatggc ggctgccgcc ggcgtgacga cctgcctcga
     9361 catggcgggt cccgtcgacg aaatcctcga gacgtccaag acctgcggcg caggcatcaa
     9421 cgtcgcgatg ctcgaaggct tctcgcccat gaagcactgc ggcacgatga cgcccacgcg
     9481 cgagcagctc gagaagtttg tccgtgaaag cctcgaaaag ggcgccgtcg gcgtcaagat
     9541 catgggcggc cactggccgc tgccgcttga aacgagccgc gaactcgtca agaccgccaa
     9601 cgacatgaac gcctacgtcg cctggcacgc cggttctcac actgcgggct ccaacatcct
     9661 cggcatgcgc gaggtcatcg aggccgccaa gggccagcgc ctgcacctcg cccacatcaa
     9721 cgcttactgc cgcggccgcg tcaatcccgt cgacgaagag tcgaaggaag ccatcgagat
     9781 gctgcgcgcc aacccgaacc tctggtgcga agcctacgtg agccccaaca acggaaccgt
     9841 gcttgactgc gacgaggacg gccagatcat cgaccacgtc acccgcacct gcctcgagac
     9901 gttcggcctc acgcccgacg ccgccggcat gcgcgaagcc ttcctcacac accgctgctt
     9961 cgcgatcgcc gacacgggct tcatgtccga actcgtcgag ggcgaggccg cgctcgaact
    10021 ctgggaaaag cagggcatga agggcgcagg cagcttcccc gtcaaccccg cactctcccg
    10081 cttcatggtc gccacggcca agcgcgagga cggtagcttc gtggtcgacg ccatctccac
    10141 cgacggcggc tgcattccgc gcaacgtcgc catctcggtc ggcctctcgc tcgtcaagtt
    10201 cggcgccctg acgctccccg agttcgtcgt caagacctcc gtcaacccag cacgccacct
    10261 gcgcctgcat gaccgcggcc acctctccga aggcgccgcc gccgacatca ccgtcttcga
    10321 ctacgaccgc cagcaggccg tcgagacgat cgtcgccggc aagtccgtca tgaagaacgg
    10381 cgagatcacg ggcaccggca tcaccctcgt cacgacgaag gcgggcatcc cggctgccga
    10441 acgcaacggc ttcaagtgga tcgaaaccga gttcgaaagc cccgaacccg aacgctacat
    10501 cccctaagcc gcattcgact ccatgagccg aagaccgccg caggcccgcc ctgcggcgtt
    10561 tttttggctt ttactcccat tcacgcccgc agcattcctt cctttggcgc gaa
//
LOCUS       DBMDTI010000046        10485 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1849_length_10485_cov_2.045551, whole genome shotgun sequence.
ACCESSION   DBMDTI010000046 DBMDTI010000000
VERSION     DBMDTI010000046.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10485)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10485
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1849_length_10485_cov_2.045551"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="ACZPB6_03790"
     CDS             complement(<1..211)
                     /locus_tag="ACZPB6_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183629.1"
                     /translation="MFSKTTRLQILLVWASIPEQPVSPGKSPAFHMIPGGAASNRFRF
                     NIADVHRRLIILKMRVPQAFVVFAEQ"
     gene            393..683
                     /locus_tag="ACZPB6_03795"
     CDS             393..683
                     /locus_tag="ACZPB6_03795"
                     /inference="COORDINATES: protein motif:HMM:NF037081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1183630.1"
                     /translation="MNKKEIAQAVAERLVLSPSLVEGIVDAVHEEIVEAMARGERVTF
                     WNFGRWELKTTAARVGREFKTGKSVVIPERNKVCFTASPNLVAAVEDAMKTL"
     gene            963..2288
                     /locus_tag="ACZPB6_03800"
     CDS             963..2288
                     /locus_tag="ACZPB6_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HIR1183631.1"
                     /translation="MGYFPQWKLKEKGVVAPDERLPTGQTVAMGVQHVVAMFGSTILA
                     PILMGFDPNMAVLMSGIGTLIFFAIVGGRVPSYLGSSFAFIGVVIAATGYVAGSGLNG
                     NIPVALGGIVVCGLVYCVVGLIVMLTGVKWIEKLMPPVVTGAVVAVIGLNLAPTACRS
                     VGPTDFDAWMAVVTFMCVGGVAVWTRGFIQKLLILIGLVIAYFIYAVLANGFGMGKPI
                     DFSIISNAAWFGVPTVHTPVFEMHAIALIVPVVIILVAENLGHVKAVTAMTGRNLDPY
                     MGRAFLGDGIATMLSGSFGGTGVTTYGENIGVMAATRVYSTLLFPVAAIFAIILGFSP
                     KFGAVIQTIPQPVLGGTSIVVFGLIAVAGARVWVVNKVDFGNSRNLIVAAVTLILGGG
                     DFTLNLGFFSLGGIGTATFGAIILNAVMSLGEPKEEKASGEPIELDRKA"
     gene            complement(2683..3162)
                     /locus_tag="ACZPB6_03805"
     CDS             complement(2683..3162)
                     /locus_tag="ACZPB6_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183632.1"
                     /translation="MSDNDKNRIKIKLPAVSYDIVKLAATLTGTSVKSFAAQAVINAA
                     LHVTAEIENRSLGSNIEADIHLTEEEAQALLALVESLDTHDGKDEDVEEATAEDFEEE
                     EPAECECDCEVECKCDCDCKCEDDAECKCEESEKAEEPAAEAAPAAQPKARHGRNHR"
     gene            3452..3928
                     /locus_tag="ACZPB6_03810"
     CDS             3452..3928
                     /locus_tag="ACZPB6_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183633.1"
                     /translation="MSEGTTRGPRTVAGRVRTLLLLAACASMLGGCAVGSGEVRDLST
                     EKAARLIIDGRTTMAQIDAELGEPDYEIHMGHEIVRHYSWMRGRPSAKNFIPFNPISE
                     FPITQKNLRIWFDKTGVVKRHEFTGVFYIYRAPLVGTDAPHSFRPLTPAELDHFSE"
     gene            3934..4491
                     /locus_tag="ACZPB6_03815"
     CDS             3934..4491
                     /locus_tag="ACZPB6_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183634.1"
                     /translation="MTIDFKRRTLGKAAAILSASLGAPMLAGCSGPQQHADNRMMPGT
                     VFTPEALKATAKGKTYVRDFVAVYGRPLRRMNDHVRHPDQKGYVWEWVATRPSFCSNL
                     NERPYMTPTVKKEIHVWRRNSDAVVTDVEVVGTFYVQVKHKMLPLVITDIRPLTRDEL
                     TASRVPAGPGENPREIELWYQSQSR"
     gene            complement(4777..6045)
                     /locus_tag="ACZPB6_03820"
     CDS             complement(4777..6045)
                     /locus_tag="ACZPB6_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183635.1"
                     /translation="MLRFPVPGAPLTPTDNERTLMKPLIRILEKGSIRWGIGCADWLP
                     IDPQHCAAEIGLNLRHVKLSEIGDTAELEAFFDEFANPEAPCLFLSDIDVPVLAAWDD
                     EEEREAMRGIIVRGLARIAATHGHHFIVTGLTSLTKDICREAFENAEPLAPHETEELF
                     TISHEQVGLLYGADLARISEERTMVPDLVMMELDRHAALGARYVEPYILQWVRQGKAG
                     ILPPVSGALTPRFLERLQNAGLMDLKRMALFQEIPAVFGKDRLDLLAEAMVTLGFWTP
                     LPAGAADEQPDDVRLYDYASVWGVRLLAVLALRFVMSEGADWMTPTRELMRAMWSGEP
                     NEAMDALCEMLDCIEDQDADLDIGGLCAACILALDLDLTRVVNEDEGLDILAVRLPGG
                     ICELVFAPEDRDPAELGSPDGTPRMVLTIG"
     gene            complement(6286..7626)
                     /gene="brnQ"
                     /locus_tag="ACZPB6_03825"
     CDS             complement(6286..7626)
                     /gene="brnQ"
                     /locus_tag="ACZPB6_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HIR1183636.1"
                     /translation="MQNTDNKRFVAIGLMLFAILFGAGNLIFPASMGQASGDHFVWAF
                     IGFCITGVGLPLLAIMAMAYAGCNDLQEAAGRAHPLYGLFYTVVVYMSIGPCFAIPRT
                     GTVAFEIGLRPFLTEGQVEMGLWIFLVLFFVVSYWLAATPQKLVDRVGKILTPALVLT
                     LGALIVKSVVDPLGVPQAAQPNYAGVLTATVAGILDGYNTLDAIAAFVFATLVTNFVR
                     EGGAKTAKEVTSQVYKSGILAVSLLAVIYFFIAKIGAESITAIGMQDTGAPVLAETAK
                     ILFGNFGAILLSVIVLLACLTTSIGLITCCAAYFMRIVGRLTYKQYCLLFTIVSFLIG
                     MFGLKTIIVSTIPVLMFVYPLCVILIVLLFTDKLFDGRQCVYAFTTAFTFVMAAVSGL
                     ETAGVNLGAISEAIKAYVPFAQYGLAWVPFAVTGYVLGLAYKAAVPCKACCKPA"
     gene            complement(8131..9309)
                     /gene="ppk2"
                     /locus_tag="ACZPB6_03830"
     CDS             complement(8131..9309)
                     /gene="ppk2"
                     /locus_tag="ACZPB6_03830"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HIR1183637.1"
                     /translation="MAKDHSHKSAVEPTGGGTLEVVDVERKPSYPVAPLEPKHVEPHS
                     FDSMTQVVTYLRDKLRDTPLVSPEESAAEVFKMLNRDLTQTERDALFKLLLVKEAHQA
                     GLFPKKPIDMNALSDKLREGAYPYKNRYPNKLYEKELYDLQVELLKLQDWVKRTNSKI
                     IVLFEGRDAAGKGGTIRRFTENLNPRGARTVALSKPTENEAGQWYFQRYCAHLPNPGE
                     ICFFDRSWYNRAVVEPVMGFCTPEQTEIFLNEVTGFEQSLIRGGTHIIKFWLAVSQDE
                     QRRRFRERRENPLKRWKLSPVDIASMDRWDDYSKAIRRMFQMTDTAETPWTVIRNEDK
                     RRGRLNAIRVLLQSIPYDNRDMKKVGNIDPLIVGRAGSLFASNGDPLRFDINNEIGNS
                     "
     gene            complement(9479..>10485)
                     /locus_tag="ACZPB6_03835"
     CDS             complement(9479..>10485)
                     /locus_tag="ACZPB6_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1183638.1"
                     /translation="PEEIVAKIREAGIVAPLAVAKYLKDANRIPERDFCIVAFRMEEC
                     PEFGRAYAGSLALTGQLNPFELELPHRDTGKLLKDVLLERGVDVERIASGEPLIDLSK
                     IAAFVELHIEQGPRLDKEPVNRAAVVTGIRGYLRHKRCRVEGETAHSGAVDYEFRHDA
                     VAALTDLLYVMNRHWEAWLHDGKDLVFTCGVINTPSTADISIISGDVSFGLDMRSLSM
                     ETIRAFHDLLLEEAAAIEEKHGVKFIFDDVIVGEAARPGAILFNRLVKAATDHGIPHL
                     QMASGAGHDAAVMTSAGVPTCMLFVANQNGSHNPHEAMRISDFLSGAEILMHAVVDYD
                     "
BASE COUNT         2136 a         3009 c         3308 g         2032 t
ORIGIN      
        1 gttgctcagc aaacactaca aacgcctgtg gcacgcgcat tttcaaaatg atcagcctcc
       61 tgtgaacgtc agcaatgtta aatcgaaacc gatttgaggc cgcaccgccg ggaatcatgt
      121 gaaatgcagg ggatttcccg ggactgacgg gctgttcagg aatggaggcc cagacgagaa
      181 gtatttgcag gcgagtcgtt ttggaaaaca ttacaattcg tctgggtgac acccccaagc
      241 atatttcccc gcaggtacaa ttgggatgtc accttaacgt gacaggcgga ccacaacaca
      301 gacgttccac aggaccggaa cggaaagcgt cgccgaaaga gggtgcccgg acgggcagcc
      361 cgtccccaag aataaagaga aggactaccg caatgaacaa gaaggaaatc gcgcaggctg
      421 tcgcagaacg cctcgtgctc tcgccctccc tcgtagaggg catcgttgat gccgtgcatg
      481 aagagatcgt cgaggccatg gcccgcggcg aacgcgtcac gttctggaac ttcggccgct
      541 gggaattgaa gactaccgcc gctcgcgtcg gtcgtgaatt caagaccggc aagtccgtcg
      601 tgattccgga acgcaacaag gtctgcttca ccgccagccc gaacctcgtc gcggccgtcg
      661 aagacgccat gaagacgctc tgacgctcag gcaccagaga gacaaaaggg gacgccgtcc
      721 gaaatggatg acgtcccttt tttgcgcacg caggcttcgc tccggagcgg cgtccgcccg
      781 tctagtcaaa acaccttatg tttatttttc gaaatgtgtg cttataatac gaaagtttgc
      841 acagacgatt cgtccttagg aggaaccctt acgactcttt gagcgcgagg cgttttgtcc
      901 ggaaagccga aggacgaggc gcctgtgcgt gtagattcaa cgtgtttgag gagtaaggaa
      961 aaatgggtta tttcccgcag tggaagctca aggaaaaggg cgtggtggcc cctgacgagc
     1021 gtcttcccac cggccagacg gtggcgatgg gcgttcagca cgtggtggcc atgttcggct
     1081 ccaccattct cgccccgatt ctcatgggct ttgaccccaa catggcggtt ttgatgagcg
     1141 gcatcggcac gctcattttt ttcgccatcg tgggcggcag ggtgccgagc tatctgggtt
     1201 catcctttgc gttcatcggc gtggtgatcg cagcgacggg ctacgttgcg ggctcgggcc
     1261 tcaacggcaa cattcccgtt gcgctgggcg gcatcgtggt ctgcggtctc gtctactgtg
     1321 tcgtgggcct catcgtgatg cttacgggcg ttaagtggat tgaaaagctg atgcctcccg
     1381 tcgtgacggg ggcggtcgtc gccgtgatcg gcctcaacct cgccccgacg gcctgccgtt
     1441 cggtgggtcc gacggacttc gacgcctgga tggccgtcgt caccttcatg tgcgtgggcg
     1501 gcgttgccgt gtggacgcgc ggcttcatcc agaagctcct catcctcatc ggccttgtga
     1561 tcgcgtactt catctacgcg gtgctcgcca acggcttcgg catgggcaag ccgatcgact
     1621 tctcgatcat ctccaacgcc gcctggttcg gcgttccgac ggttcacaca ccggtcttcg
     1681 agatgcacgc catcgcgctc atcgttccgg tcgtgatcat tctcgttgcc gagaacctcg
     1741 gccacgtgaa ggccgtgacc gccatgacgg gccgcaacct tgacccgtac atgggccgtg
     1801 cgttcctcgg cgacggcatc gcgacgatgc tctcgggctc cttcggcggc acgggcgtga
     1861 cgacctacgg tgaaaacatc ggcgtgatgg ccgccacgcg cgtctactcc acgctgctct
     1921 tcccggtcgc cgcgatcttc gcgatcatcc tcggcttctc cccgaagttc ggcgctgtga
     1981 tccagacgat tccgcagccc gtcctcggcg ggacctcgat cgtggtcttc ggcctcatcg
     2041 ccgtggccgg cgctcgcgtc tgggtcgtca acaaagtcga cttcggcaac agccgcaacc
     2101 tgatcgtggc tgccgtcacg ctgattctcg gcggcggcga cttcaccctc aacctcggct
     2161 tcttcagcct cggcggcatc ggtaccgcga ccttcggcgc gatcatcctc aacgccgtca
     2221 tgtcgctcgg cgaacccaag gaggaaaagg cctcgggcga gcccatcgag cttgaccgca
     2281 aggcctgacg cctgatctga cttcaagtcc cgaacggggc tgcttctcaa gggagcggcc
     2341 ccgtttttcg ttaatggcaa aggagagcag gttgggaagg gaggcggcaa agtcggaccg
     2401 tctgaaacct gcaagtcggc aaaagatcac tcaaggcaca gaaacgatgg acgatcgcgt
     2461 gcgcaggctg ctttatctgg gcctcaccag ggtgtccgca cgcatcgagc ttgtcgtgag
     2521 caccaaggcc atggccgcac tcaatgcggc ggcaggctga ccatcggaag tacgcaggtc
     2581 gcctgaggcc cgacgaatga tggaaaagag aaggggaccc gttgttgatg gatccccttt
     2641 gtgttctcag gtcccttgcg acaagggact caagtcgaaa gatcagcggt ggttgcggcc
     2701 gtgcctggcc ttgggctggg cagcgggagc ggcctctgcg gcaggctctt cagccttttc
     2761 ggactcttcg cacttgcatt cggcgtcgtc ctcgcacttg cagtcgcagt cacacttgca
     2821 ttcgacttcg caatcacatt cgcattccgc gggttcttct tcctcgaagt cctcggcggt
     2881 ggcttcctcg acgtcttcgt ccttgccgtc gtgcgtgtcg aggctttcga cgagggcgag
     2941 aagagcctga gcctcttcct cggtgagatg aatgtcggct tcgatgttgg agccgaggga
     3001 gcggttttcg atttcagccg tgacgtgcag ggctgcgttg atcaccgcct gggccgcgaa
     3061 ggacttcacg gacgtaccgg tgagagtggc ggcaagtttc acgatgtcgt aggagacggc
     3121 gggaagcttg atcttgatgc ggttcttatc gttgtcggac atgaccttta cctccggagg
     3181 ccaacacttt gaaaaatgct gaacggggcg tgagaaccac gccgacgttt atgacaacta
     3241 taataactcg acttaatgaa atttgcgcgg caaaagtgtg aacgttggtc aggaacttcc
     3301 cctgaaccac gctcgttttg ggctgtcggc ccccttgagg ctgcctctgc gcgaagcgag
     3361 gggtgttcaa tgggacccga gcgagttcaa aatgaatttc atcgcaccgg gcacgcacag
     3421 gactgaacga gcgctacaaa aggagaagac cttgtcggaa ggaaccacaa ggggcccgcg
     3481 gaccgtcgct ggccgcgtcc gcactctgct gcttctggca gcctgcgcct cgatgctcgg
     3541 cggctgtgcc gtcggaagcg gcgaggtgcg tgacctctcg accgagaagg ccgcgaggct
     3601 catcatcgac ggccggacca ccatggcgca gatcgatgcg gagctcggcg aacccgacta
     3661 tgaaatccac atgggccacg agatcgttcg ccactacagc tggatgaggg gacgcccctc
     3721 tgccaagaac ttcatacctt tcaacccgat ctcggaattt cctatcacgc agaagaacct
     3781 gcgcatctgg ttcgacaaga cgggcgtcgt gaagcggcat gaattcacgg gcgtcttcta
     3841 catctaccgc gcgccacttg tcggcacgga cgcgccacac agcttcaggc cgctcacgcc
     3901 cgccgagctc gatcatttct cggagtagca aggatgacga ttgacttcaa gcgcagaacg
     3961 ctcggaaagg ccgcggccat tctcagcgcc tctctcggag ctccgatgct cgcaggctgc
     4021 agcggtcccc aacaacacgc cgacaaccgc atgatgccgg gcactgtctt caccccggag
     4081 gcgctcaagg caacggccaa agggaagacc tacgtgcgcg acttcgtcgc cgtctacggc
     4141 cggccccttc gccgcatgaa cgaccacgtg cgccatcccg accagaaggg ctacgtgtgg
     4201 gaatgggtgg cgacgaggcc gtcgttttgc agcaatctca atgagcgccc ctacatgacg
     4261 cccaccgtca agaaggaaat ccacgtctgg cgacgcaaca gcgatgctgt cgtcaccgac
     4321 gtagaggtgg tcgggacctt ctacgtccaa gtgaagcaca agatgcttcc actcgtcatc
     4381 accgacatcc gaccgctcac ccgagacgaa ctcaccgctt cgcgcgtgcc tgcggggccg
     4441 ggcgagaatc cacgcgaaat cgagctctgg taccagagcc agagtcgata agctcacttg
     4501 aatagaattc cctttgagga actctattca aaaatcagga caaattgaaa gggacacttt
     4561 atcccagacg tcgaaggtcg aagtccaaag aacgcactgt ctcgctttgg caacacgtgc
     4621 cttgaagctc catccctgcg ggacgggcgc ttagagggga acgcttgttc aggacaaggc
     4681 atcatccgcc atgaaaagag ggcctgccct cggaatggag agacaggccc ttcgaaagcg
     4741 ctcaggaagt tccccaaagg agaagaagaa cgcgcgtcaa ccgatggtca ggaccatgcg
     4801 gggcgtgccg tcggggcttc cgagttcggc gggatcacga tcctcgggcg cgaagacgag
     4861 ctcgcagatg ccgcccggga gcctcacggc gaggatgtcg aggccttcat cttcattgac
     4921 cacgcgcgtg aggtcaaggt cgagcgcgag gatgcaggcg gcgcaaagcc cgccgatgtc
     4981 gaggtcggca tcctggtcct cgatgcagtc aagcatttcg cagagggcat ccatggcctc
     5041 gttgggctcg cccgaccaca tggctcgcat gagttcgcgc gtgggcgtca tccagtcggc
     5101 tccttcgctc atcacgaacc tgagcgccag gacggccagc aggcgaacgc cccagacgga
     5161 ggcgtaatcg tagagcctca catcgtcagg ctgctcgtcc gcagccccgg cgggaagcgg
     5221 cgtccagaag ccgagcgtca ccatcgcctc ggcaagaagg tcgagcctat ccttgccgaa
     5281 aacagcgggg atttcctgaa agagcgccat gcgcttcaga tccatcaggc ctgcgttttg
     5341 gagtcgctcg aggaagcggg gcgtgagcgc gccggacacg ggcgggagga tcccggcctt
     5401 cccctggcga acccattgaa gaatgtacgg ttcgacgtag cgtgcgccga gtgcggcatg
     5461 gcggtccagc tccatcatga cgaggtcggg aaccattgtc ctttcctcgg agatgcgggc
     5521 aaggtccgcg ccgtagagaa ggccgacctg ctcatgggag atcgtgaaga gctcctcggt
     5581 ctcgtgaggc gcgagaggct cggcgttctc aaacgcttcg cggcagatgt cctttgtgag
     5641 gctcgtgagg cccgtcacga tgaaatgatg tccatgtgtc gcggcgatgc gtgcaagccc
     5701 gcgcacgatg atcccgcgca ttgcctctcg ctcctcctcg tcgtcccagg ccgcaaggac
     5761 gggaacgtcg atgtcggaaa ggaagaggca gggcgcttcc ggattggcaa actcatcaaa
     5821 aaaggcttcg agttcggccg tatccccaat ttcactcagc ttgacgtgcc tgagattgag
     5881 cccgatctcg gccgcgcagt gctgcggatc gatggggagc cagtcggcgc agccgatgcc
     5941 ccagcggatg ctgccttttt ccaggatgcg gatgaggggc ttcatgagcg tacgctcgtt
     6001 gtctgtgggc gtcagaggcg cgccgggaac gggaaatcgg agcatgcgat ggatccttga
     6061 tgggggaacg gtgcccgtag tttatctgaa agagaatcgg accacgcaga cggtctgctg
     6121 cagggatcgc ctgaaggagg cggagaggtc ctcgaatacg gtgaagagag gcgcaaaagc
     6181 ccgaaagccg gaaaagagaa ggccccgcgc ttcggggaaa agtgcggggc cctttgggtt
     6241 ttctcagtga gtcctgaccg ggggcttcac ttgagagtca ggttgtcagg ccggcttgca
     6301 gcaggccttg caggggacgg cggccttgta ggcgaggccg aggacgtagc cggtgacggc
     6361 gaacgggacc caggcgaggc cgtactgggc gaacgggacg taggccttga tggcttcgga
     6421 gatcgcgccg aggttcacgc cggcggtttc gagaccggag accgcggcca tcacgaacgt
     6481 gaaggcggtg gtgaaggcgt agacgcactg gcggccgtcg aagagcttgt ccgtgaagag
     6541 cagcacgatc aggatcacgc agagcgggta gacgaacatc aggaccggga tcgtggagac
     6601 gatgatggtc ttgaggccga acatgccgat caggaacgag acgatcgtga agagcaggca
     6661 gtactgcttg tacgtcaggc ggccgacgat gcgcatgaag taggcggcgc agcaggtgat
     6721 gaggccgatg gacgtcgtca ggcaggcgag cagcacgatg accgagagaa ggatggcgcc
     6781 gaagttgccg aagaggatct tggcggtttc agcgagcaca ggagcacccg tgtcctgcat
     6841 gccgatggcg gtgatggact cagcgccgat cttcgcgatg aagaagtaga tcacggcgag
     6901 gagcgacacg gcgaggatgc cggacttgta gacctgggag gtgacttcct tcgcggtctt
     6961 ggcaccgcct tcacggacga agttcgtgac gagcgtggcg aagacgaacg cggcgatggc
     7021 gtcgagcgtg ttgtagccgt caaggatgcc cgcaaccgtg gcggtgagga cgcccgcata
     7081 gttcggctgg gcggcctgcg gaacgccgag cggatctacg acggacttga cgatcagagc
     7141 accgagcgtg agcacgaggg ccggcgtcag gatcttgccg actcggtcga cgagcttctg
     7201 cggcgtggcg gcaagccagt aggaaaccac gaagaagagg acgaggaaga tccacaggcc
     7261 catttcaacc tgaccttcgg tgaggaaggg acgcaggccg atttcaaaag ccaccgtgcc
     7321 cgtacgcgga atggcgaagc aggggccgat ggacatgtag accacgaccg tatagaacag
     7381 accataaagg ggatgtgcgc ggccggcagc ttcctgcagg tcattgcatc ccgcataagc
     7441 catcgccatg atggcgagaa ggggaaggcc tacacccgta atgcagaagc caatgaaagc
     7501 ccaaacgaag tgatcgccgg aagcctggcc catggaggcg gggaagatga gattgcctgc
     7561 gccgaacagg atcgcgaaca gcatcaggcc gatcgcgaca aagcgcttgt tgtctgtgtt
     7621 ttgcatagct tttctaatgc gggaggtgtt gtaggactct tgtgtggggc gccttcggga
     7681 ccgtccgtcg ttttcagcag agacggtgcg ggccttgtga ccgatgccct tgaataggat
     7741 tatatggaca tgtagcttat ggttacatag ggcaaacagc ttacatgagg cggatttctg
     7801 caaggttccg tcactttgaa cccgttgcgg gacatggatt ccgcccaaaa aaggctctgc
     7861 tgtttcgcaa aagttgcagg aatcaacgcc tgaaatcgca ccttctgccg acggcagccg
     7921 ggccttcaga ggcgcaaagc gcataatttt atatgtgggg cgcgcgcttt gggggcatcg
     7981 ttcccgatcg ggcgccaaac gcccgattca gttcgcatga cgtgcggcag tggccgaaag
     8041 cccacggtca gcgataagag gagggtgaaa aaatcaaagg gcccgaagat gtcgtatctg
     8101 cgggccctcg aaacaacggt ttcaggtcga tcagctgttg ccgatctcgt tgttgatgtc
     8161 aaagcgcagc ggatcaccgt tagaggcgaa gaggctgcct gcgcgaccga cgatgagcgg
     8221 gtcgatgttg cccaccttct tcatgtcgcg gttgtcgtac ggaatcgatt gcagaagcac
     8281 gcggatggcg ttgagacgac cgcggcgttt gtcttcgttg cggatcaccg tccagggcgt
     8341 ttcggccgtg tcggtcatct ggaacatgcg gcgaatggcc ttcgaatagt cgtcccagcg
     8401 gtccatggac gcgatgtcga cgggagagag cttccagcgc ttcaacgggt tctcgcggcg
     8461 ttcgcggaag cggcggcgct gttcgtcctg gctcacggcg agccagaact tgatgatgtg
     8521 cgtgccgccc ctgatgaggg actgctcgaa gcccgtcact tcgttgagga agatttcggt
     8581 ctgctcgggc gtgcagaatc ccatcacggg ttcgaccacg gcgcggttgt accaggagcg
     8641 gtcgaagaag cagatctcgc ccggattggg caggtgcgcg caatagcgct ggaagtacca
     8701 ctggcccgct tcattctcgg tcggcttcga aagggccacc gtgcgcgcgc cgcgaggatt
     8761 gaggttctcg gtgaagcggc ggatcgtgcc gcccttgccg gccgcgtcac ggccttcaaa
     8821 gagcacgatg atcttcgagt tcgtgcgctt gacccagtcc tgaagcttca gaagctcgac
     8881 ctgcaggtca tagagttcct tctcatagag cttgttcggg tagcggttct tgtacggata
     8941 ggcgccttcg cggagcttgt ccgaaagagc gttcatgtcg atgggcttct tcgggaagag
     9001 gcccgcctga tgcgcctcct tgacgagcag cagcttgaag agcgcgtcac gttccgtctg
     9061 cgtcaggtcg cggttgagca tcttgaacac ttcggcggcc gactcttcgg gggagacgag
     9121 cggcgtgtcg cgaagcttgt cgcgaagata ggtgacgacc tgtgtcatcg agtcgaacga
     9181 atgaggttcg acatgcttgg gttcaagggg agccacagga taggagggct tcctttcaac
     9241 gtctacgact tcaagggtgc cgccgccggt gggttcgacg gcagacttgt gagaatgatc
     9301 cttggccatg tgcgtcctcc tttctgattt ttactgtcac gcataagcac gtgggataag
     9361 tgtacaaccg cctgtaacga aatgttatga gcaatgtccc gtaaccgtca cgacgcgacg
     9421 gccctgactc agggaatcgt cgcaggcgtt gtgtgaggga gaatggctcc cgtttccgtc
     9481 aatcataatc cacgacggcg tgcatgagaa tttccgcacc tgagaggaaa tccgaaattc
     9541 gcatcgcctc atgcgggttg tgagacccat tctggttggc gacgaagagc atgcacgtgg
     9601 gcacgcccgc gctcgtcatg actgcggcgt cgtggcccgc tccgctcgcc atctgaagat
     9661 gaggtatgcc gtgatccgtc gcggccttga cgagccgatt gaaaaggatt gcgccgggac
     9721 gtgccgcctc gcctacgatg acgtcgtcaa agatgaactt cacaccgtgc ttttcttcga
     9781 tggcggccgc ctcctcaaga aggaggtcgt ggaaggcgcg gatcgtctcc atcgaaaggc
     9841 ttctcatgtc gagcccaaag gaaacgtcgc ccgagatgat tgagatgtcg gccgtcgaag
     9901 gcgtgttgat gacgccgcag gtgaagacca gatccttgcc gtcatggagc caggcctccc
     9961 agtgcctgtt catcacgtaa agcaggtccg tcagggccgc gacggcgtcg tgcctgaatt
    10021 cataatcaac ggcgcccgaa tgcgcggtct cgccctcaac gcggcatcgc ttgtgcctca
    10081 gatacccgcg gattcccgtg acgaccgccg cacggttgac aggttccttg tcaagcctcg
    10141 gaccctgctc aatgtgaagc tcgacaaagg ctgcaatctt cgagagatcg atgagcggct
    10201 ctcccgacgc gatacgctcg acatcgaccc cgcgctcaag gagaacgtcc ttcagaagct
    10261 tccccgtatc cctgtgagga agttctagct cgaagggatt gagctgcccc gtcagggcga
    10321 gactgcccgc ataggcacgt ccaaattcag ggcactcctc catgcggaat gcaacaatac
    10381 agaagtcccg ctcaggaatt ctatttgcat ccttcagata tttggccacg gcgagcggcg
    10441 cgacgatgcc ggcttcgcga atttttgcca cgatttcttc cggcg
//
LOCUS       DBMDTI010000047        10307 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1887_length_10307_cov_3.020478, whole genome shotgun sequence.
ACCESSION   DBMDTI010000047 DBMDTI010000000
VERSION     DBMDTI010000047.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 10307)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10307)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10307
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1887_length_10307_cov_3.020478"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            113..1906
                     /gene="lepA"
                     /locus_tag="ACZPB6_03840"
     CDS             113..1906
                     /gene="lepA"
                     /locus_tag="ACZPB6_03840"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016723245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR1183639.1"
                     /translation="MQKIRNFSIIAHIDHGKSTLADRLIQRCGGLSDREMSAQVLDSM
                     DLERERGITIKAQTASLKYKAKDGEVYELNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DATQGVEAQTVANCYTAIDLGVTVLPVLNKMDLQSANPDAAAEEIEDVIGIDATDAIP
                     CSAKTGMGIDDILERVVRDVPPPKGDPDAKLQALVIDSWFDNYVGVVMLVRVVNGTLR
                     PKDKIRLMATGANHLVEQLGVFTPKSQSRTSLSAGEVGFVIAGIKELKDARVGDTVTS
                     AQNPADEAVPGFKEVKPQVFAGLYPVESNQYDALRDALTKLQLNDAALQFEPEVSQAL
                     GFGFRAGFLGLLHMDIVQERLEREYNMDLITTAPSVVYEVLQTDGTVVHVENPSKLPP
                     VDKIDEIREPIDTVTIFVPDEYVGAVMKLCQDKRGIQTNLAYHGRQVHLTYELPLAEI
                     VLDFFDRMKSMTRGYASMDYEFKEYRASDVVRVDMLINGDRVDALSSILHRSNAIFRG
                     REIAQRLRSLIPRQMYEVSIQAAIGANIIARENVKALRKNVLAKCYGGDITRKRKLLE
                     KQKAGKKRMKQVGSVEIPQEAFLAILQQDEQ"
     gene            1975..2865
                     /gene="lepB"
                     /locus_tag="ACZPB6_03845"
     CDS             1975..2865
                     /gene="lepB"
                     /locus_tag="ACZPB6_03845"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1183640.1"
                     /translation="MNFPLILLILTVVTGIFWVLERRSFLPARKARAEEAARRFEADN
                     REAIDRGEKAVIDARNDLYAREIRQPWWLEYTAGLFPVIAIVFFVRSFLFEPFRIPSG
                     SMLPTLHVGDFILVNKYEYGIRLPVTNWKIIPLGSPQRGDVVVFKYPMDESVDYIKRV
                     VGVPGDTIEYRNKVVYVNGVEQKQAGHVDFIDTHSHVTLDQREENLSGVEHMIAVDGR
                     RPSWVPAQAILKKVPECTYTNTGFICKVPEGKYFMMGDNRDNSEDSRYWGFVDDSSLV
                     GRATFIWANFGDMSRIGSFR"
     gene            2902..3606
                     /gene="rnc"
                     /locus_tag="ACZPB6_03850"
     CDS             2902..3606
                     /gene="rnc"
                     /locus_tag="ACZPB6_03850"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR1183641.1"
                     /translation="MQDLSILEERIGYKFADRKLLERAVTHRSFAAQHNERLEFLGDS
                     VLNCVVGYALFQRDKHFTEGDLSRVRANLVCERTLAVIAKQIDVSAFLRMGEGEMKTG
                     GAHRPSITADAMEAIFGAVFAESGFEAAQKVILGLFEPILAAPTAEQLGKDSKTRLQE
                     YLQGRHLPLPQYVVVSVTGAAHAQHFNCECRIPSLEITTQGHAASRRSAEQEAATAAL
                     EILKNRESTNGRSRHE"
     gene            3599..4573
                     /gene="era"
                     /locus_tag="ACZPB6_03855"
     CDS             3599..4573
                     /gene="era"
                     /locus_tag="ACZPB6_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR1183642.1"
                     /translation="MSEEKTVPAMSDAELERMLAQAGAASVQVPEGFRCGYVAVIGRP
                     NVGKSTLINHLIGEKVSITSKKPQTTRDRVLGVVTEPDAQIVFMDTPGFQTKVGNQLI
                     RRMNRTVRSTLGEVDAIVFVIESLGWKPADLEVLKLLPKDASNVILAINKTDLNKSKD
                     SLLPLMAASMEKFPFAAIVPVSAEKGRQLGDLLGEIKKFLPESVPFFDPDTYTDRSPR
                     FLAAETIREKAFRLLGDELPYGIAVTIDKWQEDEESAEIVATLIVERESHKPIVIGEG
                     GAKLREIGRLARTDVAAMLGKRVHLEVWVRVRRGWSDDVKALKTLGYE"
     gene            4566..5492
                     /gene="recO"
                     /locus_tag="ACZPB6_03860"
     CDS             4566..5492
                     /gene="recO"
                     /locus_tag="ACZPB6_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR1183643.1"
                     /translation="MNKDALEAGVPHRRVRNRSEGPRSSRSPVSSLEGPAAQRASTLS
                     GILERRRAAERQMADVFSALPEPAERIPARVTGELGFILHAWDWSETSLLLDVLTVRY
                     GRVFLVARGARRASSQYRGLLTAFCPLRFSWTGSKEAKMLVRAEWQGTMVPLTGEALL
                     SGFYVNELILKLTVREERHPGLFEAYVKAIHALALDERDAMQPALREFEMAVLKAAGW
                     APAFSDDGRSPWYCVRDGALEGLAGVEGLPEGVRAWPGDLVREMLSGEPMSPAALRAS
                     RDILRTLIDFHLDGRSIHSRRILAELNGLQKT"
     gene            5564..6325
                     /locus_tag="ACZPB6_03865"
     CDS             5564..6325
                     /locus_tag="ACZPB6_03865"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR1183644.1"
                     /translation="MLRTKLSVNLNKVALLRNSREGNWPDPVFFGRIALEAGAAGLTV
                     HPRPDERHVRATDVPAIAALVSEYPDREYNIEGNPFHNLMGHLEAVRPDQATFVPDSL
                     GQKTSDHGFDLSDPAVCDAVRPLIAQAKAWGCRVSLFMDPAPALMAKAAELGADRVEL
                     YTEGWAQAHGTEAQDKVLEQYAASVKAAREAGLDVNAGHDLSLDNLEVFLKACREVSE
                     VSIGHALTAEALQFGFAETVRRYEEILDRVAASED"
     gene            6341..7318
                     /gene="nagZ"
                     /locus_tag="ACZPB6_03870"
     CDS             6341..7318
                     /gene="nagZ"
                     /locus_tag="ACZPB6_03870"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR1183645.1"
                     /translation="MIEVLKPGPVCVDVEGLSLTEHERGRLRHPMTGMVILFTRNYRD
                     REQLRALCDEIHAVRPGILISVDHEGGRVQRFRSEFTDVPAMSEIAAHEDAEARFEAA
                     GLVLAAELRACGVDFTFAPVLDVDYGRSAVIGSRSLGTTPELVTAHAAALIRGLRRGG
                     MASCGKHFPGHGWAEADSHTALPEDERDAESLMRDMLPYGKLPELASVMTAHVAYHAF
                     EGEVATFSRRLLQDELRERLGFRGLVFSDDLSMKGAAGGSVTEQAEKALAAGCDMVLV
                     CNRPDMADELLANLRWTRTPEFDARLAGLAPAAACGGAELEEARSLLAA"
     gene            7366..8547
                     /gene="pncB"
                     /locus_tag="ACZPB6_03875"
     CDS             7366..8547
                     /gene="pncB"
                     /locus_tag="ACZPB6_03875"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR1183646.1"
                     /translation="MIIESLLDTDLYKFTMQQCVLHQFPGADAEYRFKCRTPGIDLSP
                     FVDEINAELDHLCSLYFREDELAYLDSLRFIKSDYVEFLSLFHLKRKYISVTKSQDYP
                     CGIDITAKGPWLHTILFEIPVLAIVNEVYFRNTYPDLDETEGVARLERKIELLRNEPD
                     NGGLRISDYGTRRRFSKAWQKKVLLNMKERLGGIFTGTSNVMYAKELGLTPHGTMAHE
                     FLQACQALGPRLRDTQVFAFEMWAKEYRGDLGIALSDVYGMDPFLRDFDMFFCKLFDG
                     VRHDSGDPFVWGERMIEHWKANRCDPRTKSLIFSDSLTVPKAIELYERFHGRVKVAFG
                     IGTNLMNDRGPQPLNVVMKMVKANGQPVAKISDAPEKGMCEDEGYLRYLRQVFGLPPV
                     A"
     gene            8897..10246
                     /locus_tag="ACZPB6_03880"
     CDS             8897..10246
                     /locus_tag="ACZPB6_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HIR1183647.1"
                     /translation="MASDMNGAELGLAWAVPFAGILLSIALFPLFAPHIWHHHFGKIS
                     VFWALCCIIPLATVFGPDTAFHEIAHVLLADYVPFIIFVGSLFIVAGGIHVRGSFVGR
                     PIVNTAILALGAVLANFMGTTGAAMLLIRPLIGANENRRRKVHTFIFFIFLVANVGGA
                     LTPLGDPPLFLGFLKGVSFFWTLEHLMLPWLVTCAILLAVYFAIDSFMFSRDVKDGFK
                     APEEKQAIGVDGGINILFLAAIVGTVLMSGFWRSGVDFTFLGVHFSLEGVLRDALFIV
                     ISGASLLLTTKATREANHFSWDPILEVAKLFFGIFCCIVPVLAMLRAGHDGHFAPLVA
                     LVTNADGSFNNTFFFWLTGMLSAFLDNAPTYLAFFNLAGGDPTVLMGEGAKTLMAISM
                     GSVFMGAVTYIGNAPNFMTVSICNERGIKMPTFFGYMLWSVTILFPTFFLMDMLFLI"
BASE COUNT         1828 a         3550 c         3091 g         1838 t
ORIGIN      
        1 aggtcctaga aaggcccgat gacggccatg ttttgcaaga gccgcccgtt cccgtcgagc
       61 attgcgctcc ggccggcggc ccgtacaaat tccgacccat tcagtctctt caatgcagaa
      121 aatccgcaac ttctcgatca tcgcccacat tgaccacggc aagtcgacgc tcgccgaccg
      181 cctgatccag cgctgcggcg gtctttccga ccgcgaaatg agcgcccagg tgctcgacag
      241 catggacctc gagcgtgaac gcggcatcac gatcaaggcg cagaccgcct ccctcaagta
      301 caaggccaag gacggcgagg tctacgaact caacctcatc gacactccgg ggcacgtcga
      361 cttctcctat gaagtgagcc gttcgctctc cgcgtgcgaa ggcgcgctgc tcgtcgtcga
      421 cgctacgcag ggcgtcgagg cccagacggt tgcgaactgc tacaccgcca tcgacctcgg
      481 cgtgaccgtg ctccccgtcc tcaacaagat ggaccttcag agcgcgaacc ccgatgcggc
      541 cgccgaggaa atcgaggacg tgatcggcat cgacgcgacg gacgcgattc cctgctcggc
      601 caagaccggc atgggcatcg acgacatcct tgagcgcgtc gtacgcgacg ttccgcctcc
      661 gaagggcgat ccggacgcca agctccaggc cctcgtcatc gactcctggt tcgacaacta
      721 cgtcggcgtg gtgatgctcg tgcgcgtcgt caacggcacg ctccgtccga aggacaagat
      781 ccgcctgatg gccaccggcg ccaatcacct cgtcgagcag ctcggcgtct tcacgccgaa
      841 gtcccagagc cgcacctcgc tctccgcggg tgaagtgggc ttcgtgattg ccggcatcaa
      901 ggagctcaag gacgctcgcg tgggcgacac cgtgacgagc gcccagaatc ctgccgacga
      961 ggccgtgccc ggcttcaagg aagtgaagcc gcaggtgttc gcgggcctct atcccgtcga
     1021 gtccaaccag tacgatgcgc ttcgcgacgc cctcacgaag ctccagctca acgacgccgc
     1081 gctccagttc gagcccgaag tctctcaggc gctcggcttc ggcttccgcg ccggcttcct
     1141 cggcctcctg cacatggaca tcgtccagga gcgccttgaa cgcgaataca acatggacct
     1201 catcacgacg gccccgtccg tggtgtacga ggttctgcag acggacggca cggtggtgca
     1261 cgtcgagaac ccgtccaagc tcccgcccgt cgacaagatc gacgagatcc gcgagccgat
     1321 cgacacggtc acgatcttcg tacccgacga atacgtgggc gccgtcatga agctctgcca
     1381 ggacaagcgc ggcatccaga cgaaccttgc ctaccacggc cgccaggtgc acctcaccta
     1441 cgagctgccg ctcgccgaga tcgtgctcga cttcttcgac cgcatgaagt cgatgacccg
     1501 cggctatgcc tcgatggact acgagttcaa ggaataccgc gcctccgacg tggtgcgcgt
     1561 ggacatgctg atcaacggcg accgcgtgga cgccctgagc tccatccttc accgcagcaa
     1621 cgcgatcttc cggggccgcg aaatcgcaca gcgcctgcgc tcgctgattc cgcgccagat
     1681 gtacgaggtc tcgattcagg ccgccatcgg cgcgaacatc atcgcccgag agaacgtcaa
     1741 ggccctgcgc aagaacgtgc tcgccaagtg ctacggcggc gacattacgc gaaagcgcaa
     1801 gctccttgaa aagcagaagg ccggcaagaa gcgcatgaag caggtgggct cggtggagat
     1861 tccgcaggaa gccttccttg caatccttca gcaggacgaa cagtaatccc gccgcaaagc
     1921 ggaggttcga cgacggacct ccgcttcgcc gcatgaacga aacggaagac attgatgaac
     1981 tttcctctga tccttctcat tctcacggtc gtcacgggca tcttctgggt gcttgagcgc
     2041 cgcagcttcc tgcccgcccg caaggcccgc gccgaagagg ccgcccgccg cttcgaggcg
     2101 gacaaccgcg aggcgatcga tcgcggcgaa aaggccgtga tcgacgcgcg caacgacctc
     2161 tacgcccgcg agatccgcca gccctggtgg ctcgagtaca cggcgggcct cttccccgtg
     2221 atcgcgatcg tcttcttcgt gcgcagcttc ctcttcgagc cgttccgcat tccgtcgggc
     2281 tcgatgctcc cgacgctgca cgtgggcgac ttcatcctcg tcaacaagta cgagtacggc
     2341 atcaggctcc ccgtcaccaa ctggaagatc attccgctcg gcagcccgca acgcggcgac
     2401 gtcgtggtct tcaagtaccc gatggacgag tcggtcgact acatcaagcg cgtcgtcggc
     2461 gttccgggcg acacgatcga ataccgcaac aaggtcgtct acgtcaacgg cgtcgagcag
     2521 aagcaggccg gccacgtcga cttcatcgac acgcactccc acgtcacgct cgaccagcgc
     2581 gaggagaacc tttcgggcgt cgagcacatg atcgccgtcg acggccgccg tccgtcctgg
     2641 gttccggcgc aggccattct gaagaaggtc cccgagtgca cgtacacgaa cacgggcttc
     2701 atctgcaagg tccccgaagg caagtacttc atgatgggcg acaaccgcga caactccgag
     2761 gacagccgct actggggctt cgttgatgac tcgagcctcg tcggccgcgc cacgttcatc
     2821 tgggcgaact ttggcgacat gtcccgcatc ggctccttcc gctgagcgga cccggtctga
     2881 cagacaccgt ttggaataaa aatgcaagat ctttcgattc ttgaggagcg catcggctac
     2941 aagttcgccg accgcaagct tctcgaacgt gcggtgacgc accgcagctt cgcggcgcag
     3001 cacaacgagc gcctcgaatt cctgggcgac tcggttctca actgcgtcgt gggctacgcg
     3061 ctcttccagc gcgacaagca cttcaccgaa ggcgaccttt cgcgcgtgcg cgccaacctc
     3121 gtctgcgaga ggaccctcgc ggtcatcgcc aaacagatcg acgtctcggc cttccttcgc
     3181 atgggcgaag gcgagatgaa gacgggcggt gcgcatcgtc cctcgatcac ggccgatgcc
     3241 atggaggcca tcttcggcgc cgtgttcgcc gaaagcggct ttgaggctgc ccagaaggtg
     3301 atcctcggcc tcttcgagcc gatcctcgcc gcgcccacgg ccgaacagct cggcaaggac
     3361 tccaagacgc gcctgcagga gtacctgcag ggccgccacc tgccgctgcc gcagtacgtc
     3421 gtcgtcagcg tgacgggtgc cgcgcacgcc cagcacttca actgcgagtg ccgcattccg
     3481 tcgcttgaaa tcacgacgca gggccatgct gcgagccgac gctcggccga gcaggaggcc
     3541 gccaccgccg cgcttgagat tctgaagaac cgcgagtcca ccaacgggag gtccagacat
     3601 gagtgaagaa aagaccgtgc cggcaatgtc cgacgccgaa cttgaacgca tgctcgcgca
     3661 ggcgggcgcc gcctccgtgc aggtgcccga gggcttccgt tgcggctatg tggccgtcat
     3721 cggccgtccg aacgtcggca agtcgaccct gatcaaccac ctgatcggcg agaaggtctc
     3781 gatcacctcg aagaagccgc agacgacgcg cgaccgcgtg ctcggcgtcg tgaccgaacc
     3841 cgacgcccag atcgtcttca tggacacccc ggggttccag acgaaggtcg gcaaccagct
     3901 catccgccgc atgaaccgca ccgtgcgctc cacgctcggc gaggtcgatg cgatcgtctt
     3961 cgtgatcgag tccctcggct ggaagcccgc cgacctcgaa gtcctcaagc ttctgcccaa
     4021 ggatgcgtcg aacgtcatcc tcgccatcaa caagacggac ctcaacaagt cgaaggactc
     4081 gctccttccg ctcatggccg cctcgatgga gaagttcccg tttgcggcca tcgtgcccgt
     4141 gtccgctgaa aagggccgcc agctcggcga cctgctcggc gagatcaaga agttcctccc
     4201 cgagagcgtc ccgttcttcg atcccgacac ctacacggac cgctcgccgc gcttcctcgc
     4261 ggccgagacg atccgcgaga aggccttccg cctgctcggc gacgagcttc cgtacggcat
     4321 tgccgtgacg atcgacaagt ggcaggagga cgaggagagc gccgagatcg tcgcgaccct
     4381 catcgtcgag cgtgaaagcc acaagccgat cgtgatcggc gagggcggcg ccaagcttcg
     4441 cgagatcggc cgtctggctc gcaccgacgt cgccgcgatg ctcggcaagc gcgtgcacct
     4501 cgaggtctgg gtccgcgtgc gccgcggctg gtccgacgac gtgaaggccc tcaagaccct
     4561 gggctatgaa taaggacgcc ctggaggcgg gggtgccgca ccgcagggtg cgcaaccgtt
     4621 ccgaggggcc ccgctcctca cggtctcccg tctcctcgct cgaggggccc gcagcgcagc
     4681 gcgcctcgac cctctcgggc attctcgaac gccgccgcgc tgcagagcgg cagatggcgg
     4741 acgtcttctc cgcgctcccg gaaccagcgg agcgcatccc cgcccgtgtc acgggcgagc
     4801 tcggcttcat cctccacgcg tgggactgga gcgagacgag ccttcttctc gacgtgctga
     4861 ccgtacgata cggccgcgtc tttctcgtcg cgcgcggcgc ccgccgcgcg tcctcccagt
     4921 acaggggcct tctcaccgcg ttctgtccgc tccgcttctc atggacgggc agcaaggagg
     4981 ccaagatgct cgtgcgcgcc gagtggcagg ggacgatggt gcccctcacg ggcgaggcgc
     5041 tgctctcggg cttctacgtc aacgaactca tcctcaagct caccgtccgc gaggagcgcc
     5101 atccggggct cttcgaggcc tacgtcaagg cgatccatgc gttggcgctc gacgagcgcg
     5161 acgccatgca gcccgcgctt cgcgaattcg agatggcggt cctcaaggcc gcaggctggg
     5221 cgcccgcctt ctccgacgac ggccgctcgc cctggtactg cgtcagggac ggcgccctcg
     5281 aggggctcgc cggcgtcgaa gggttgcccg agggcgtgcg cgcctggccg ggggacctcg
     5341 tgcgcgaaat gctttcggga gagccgatga gtcctgcggc gcttcgcgcc tcgcgtgata
     5401 ttcttcgtac gttgatcgac tttcatctcg acgggcgcag catccattcc cgtcgcatcc
     5461 tcgccgagct caacggcctg cagaagacct gacgcccgcc aagccgatcg aaccggacaa
     5521 acagggggcg ctgcggcgtc ccgaacacca ggagtgcatc gcaatgcttc gtaccaagct
     5581 ttccgtcaat ctgaacaagg tggcgcttct tcgcaactcc cgcgaaggca actggcccga
     5641 cccggtcttc ttcggccgca tcgcgcttga ggcgggcgcc gccggcctga cggttcatcc
     5701 gcgtcccgac gagcgccacg tccgcgcgac ggatgtcccc gcgattgccg cactcgtgag
     5761 cgaatatccc gaccgcgaat acaacatcga gggcaacccc ttccacaatc tgatgggcca
     5821 ccttgaggcc gtgcgtcccg atcaggcgac gttcgtgccc gacagcctcg gccagaaaac
     5881 gtccgaccac gggttcgatc tttcggatcc cgccgtctgc gacgccgtcc gcccgctcat
     5941 cgcgcaggcg aaggcctggg gctgccgcgt gagcctcttc atggatcccg cccccgccct
     6001 gatggcgaag gccgccgaac tcggcgccga ccgcgtcgaa ctctacacgg agggctgggc
     6061 tcaggcgcac ggcaccgaag cccaggacaa ggtcctcgag cagtacgccg ccagcgtgaa
     6121 ggccgcccgc gaggccggac ttgacgtcaa cgccgggcac gacctctcgc tcgacaatct
     6181 cgaggtcttc ctcaaggcct gccgcgaggt gagcgaggtc tcgatcggcc atgcgctcac
     6241 cgccgaggcg ctccagttcg gctttgccga aacggtcagg cgctacgagg agatcctcga
     6301 ccgcgtcgcc gcgtccgaag actgaaggga ggagctgggc atgatcgaag tactgaagcc
     6361 gggtcccgtc tgcgtcgacg ttgagggcct ttcactcacc gagcatgagc gtgggcgcct
     6421 gcgtcatccg atgacgggca tggtgatcct tttcacccgc aactacaggg accgggaaca
     6481 gctcagggcg ctgtgcgacg aaatccacgc cgtgcgtccg ggcatcctca tcagcgtcga
     6541 ccatgagggc ggccgcgtgc agcgcttccg ctccgaattc accgatgtgc ccgccatgtc
     6601 cgagatcgcg gcgcacgagg acgccgaggc gcgcttcgag gcggccggtc tcgtgcttgc
     6661 cgccgagctt cgcgcctgcg gcgtcgactt caccttcgcg cccgtgctcg acgtcgacta
     6721 cggccgatcg gccgtgatcg gctcgcgcag ccttggcacg acgcccgagc tcgtgacggc
     6781 gcatgccgcc gcgctcattc gcgggcttcg caggggcggc atggcctcct gcggcaagca
     6841 ttttccgggg cacggctggg ccgaggccga cagccatacg gcccttcccg aggacgaaag
     6901 ggatgcggag agtctgatgc gggacatgct cccctacggc aaactgcctg aacttgcatc
     6961 cgtcatgacg gcccatgtgg cttatcatgc ctttgagggg gaggtcgcca ccttctcgag
     7021 gcgcctcctg caggacgagc ttcgagaacg gctgggcttt cgcggcctcg tcttctcgga
     7081 cgacctcagc atgaagggcg ccgcgggcgg cagcgtgacg gagcaggccg agaaggcgct
     7141 tgcggcgggc tgcgacatgg tactcgtatg caatcgtccc gacatggccg acgagcttct
     7201 cgcgaacctg cgttggactc gaacgcccga attcgacgcg cgccttgcgg ggctcgcgcc
     7261 cgccgcggct tgcggcggtg cggagcttga agaggcccgc tcccttctgg cggcctgacg
     7321 gccggccccg aaggcgtcaa tcattccacg tataggaagg ctgtcttgat catcgaatca
     7381 ctgctcgaca cggacctcta caaattcacg atgcagcagt gcgtgctgca ccagttcccg
     7441 ggggccgatg ccgaataccg cttcaagtgc cgcacgcccg gcatcgatct ttcgcccttc
     7501 gtcgacgaga tcaacgccga gctcgaccat ctctgcagcc tctacttcag ggaggacgag
     7561 ctcgcctacc tcgacagcct gcgcttcatc aagtcggact acgtcgagtt tctgagcctc
     7621 ttccatctga agcgcaagta catctccgtg acgaagtctc aggactatcc ctgcggcatc
     7681 gacatcaccg cgaagggccc gtggctgcac acgatcctct ttgagattcc cgtgcttgcg
     7741 atcgtcaacg aggtctactt caggaacacg tatccagatc ttgacgagac ggagggggtc
     7801 gcacgccttg agcgcaagat cgagcttctg cgcaacgagc ccgacaacgg cgggctgcgg
     7861 atctccgact acggtacgag gcgccgcttc tccaaggcct ggcagaagaa ggttctcctc
     7921 aacatgaagg agcgtctcgg cggcatcttc actggcacgt cgaacgtcat gtacgccaag
     7981 gagctcggcc tcacgcccca cggcacgatg gcccacgagt tcctgcaggc ctgccaggcg
     8041 ctgggcccaa ggctgcgcga cacgcaggtc ttcgccttcg agatgtgggc caaggagtac
     8101 aggggcgacc tcggcatcgc actctccgac gtctacggga tggatccctt cctgcgcgac
     8161 ttcgacatgt tcttctgcaa gctcttcgac ggcgtcaggc acgactcggg cgatcccttc
     8221 gtctggggcg agcgcatgat cgagcactgg aaggccaacc gctgcgaccc gaggacgaag
     8281 tccctcatct tcagcgacag cctgacggtc ccgaaggcca tagagctcta cgagcgcttc
     8341 cacggcaggg tcaaggtcgc cttcggcatc ggcacgaacc tgatgaacga cagggggccg
     8401 cagccgctca acgtcgtcat gaagatggtc aaggccaatg ggcagcccgt cgccaagatc
     8461 tccgacgcgc ccgagaaggg catgtgcgag gacgagggct atctgcgtta cctcaggcag
     8521 gtcttcggac tgccgcccgt ggcataggag tctcacgggc acgatgaaag gcgcggacct
     8581 agggcctgcg cctttcattt tccagtcctt tcggcttaag gggcccctca tttgtaaaga
     8641 aacctcggaa agttctgcag ggatcagaaa atccgtgtat gtttgcattc tgagatattt
     8701 tgtaacgcgg tgcacattgg aaagtacagt tggcgcctcc gctcccggga agcgctttcg
     8761 accgcacctt ccaacgtacg ccattttcaa ccggcggccg gcgctcaggc ggtcgcccca
     8821 gggagtctcc atcgtgactt tgtccattaa aagcgttgcg gctgcaactg caacccttct
     8881 gctgcctgct gccgccatgg cgagcgacat gaacggcgcc gagctcggcc ttgcgtgggc
     8941 cgtgccgttt gccggcattc tgctttccat cgcgcttttc ccgctgttcg ccccgcacat
     9001 ctggcaccat catttcggca agatctccgt cttctgggcc ctgtgctgca tcattccgct
     9061 tgcgaccgtc ttcggtcctg acacggcctt ccacgagatc gcccacgtgc ttctcgccga
     9121 ctacgtgccg ttcatcatct tcgtgggctc gctcttcatc gttgccggcg gcattcacgt
     9181 ccgcggcagc ttcgtcggtc gtccgatcgt gaacaccgcg atcctcgcgc tcggcgccgt
     9241 gcttgccaac ttcatgggca cgacgggcgc tgccatgctt ctcatccgcc cgctcatcgg
     9301 cgccaacgag aaccgcaggc gcaaggtcca caccttcatc ttcttcattt tcctcgtcgc
     9361 caacgtgggg ggcgcgctga ccccgctcgg cgatcctccg ctcttcctcg gcttcctcaa
     9421 gggcgtgagc ttcttctgga cgcttgagca cctgatgctt ccgtggctcg tgacctgcgc
     9481 catcctgctt gcggtctact tcgcgatcga cagcttcatg ttctcccgcg acgtcaagga
     9541 tggcttcaag gcgcccgagg aaaagcaggc catcggcgtc gacggcggca tcaacatcct
     9601 gtttctcgcc gccatcgtcg gcacggtcct gatgtccggc ttctggcgct cgggcgtgga
     9661 cttcacgttc ctcggcgtgc acttctcgct cgagggcgtg ctccgcgacg cgctcttcat
     9721 cgtgatctcc ggcgcttccc tgctgcttac cacgaaggcc acccgcgaag ccaaccactt
     9781 cagttgggat ccgatccttg aggtcgccaa gctcttcttc ggcatcttct gctgcatcgt
     9841 gccggtgctc gccatgctcc gcgcgggtca cgacggccac ttcgcgccgc tcgtcgccct
     9901 cgtgacgaac gccgacggca gcttcaacaa cacgttcttc ttctggctga cgggcatgct
     9961 ctccgcgttc cttgacaacg cgccgacgta tctggcgttc ttcaacctcg ccggcggcga
    10021 tccgaccgtg ctgatgggcg agggcgccaa gaccctgatg gccatctcga tgggctccgt
    10081 gttcatgggc gccgtgacct acatcggcaa cgccccgaac ttcatgacgg tctccatctg
    10141 caacgagcgc ggcatcaaga tgccgacgtt cttcggctac atgctctggt cggtgacgat
    10201 cctctttccg accttcttcc tgatggacat gctcttcctc atctgagagc cgtcctgaag
    10261 aaaacaggct gaaacaaggc tgaaaaacag gcgccccgaa gcgaagg
//
LOCUS       DBMDTI010000048        10227 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1902_length_10227_cov_3.389690, whole genome shotgun sequence.
ACCESSION   DBMDTI010000048 DBMDTI010000000
VERSION     DBMDTI010000048.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10227)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1902_length_10227_cov_3.389690"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(350..1087)
                     /locus_tag="ACZPB6_03885"
     CDS             complement(350..1087)
                     /locus_tag="ACZPB6_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR1183648.1"
                     /translation="MEYLFDLLANLHWAAVGQIIMIDILLGGDNAVVIAMACRNLPES
                     QRNKGIFWGTAGAIALRVVLITCAVMLLDLPFLKVAGGALLVWIGAKLLLPEDGDHEK
                     VEGSSKLIGAIKTIIVADFVMSFDNVIAIAGAAQQAHETHQTLLIIFGLLVSVPFIVF
                     GSQIILKLIDRFPAIIYFGAGLLGWIAGGMIASDVFVTTRWPEQAFMMHYPLSVGCAA
                     LVILIGHFLGKAKMKKLLAKEMEKDRS"
     gene            complement(1233..2051)
                     /gene="recX"
                     /locus_tag="ACZPB6_03890"
     CDS             complement(1233..2051)
                     /gene="recX"
                     /locus_tag="ACZPB6_03890"
                     /inference="COORDINATES: protein motif:HMM:NF001055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HIR1183649.1"
                     /translation="MGRNWEEGELFVWGDDGLVPADSQEKAVRSAAEPARALPLETPP
                     VRRSEEPAVRRVGRSSGLGEIVTGVDEASGIVRTAPKVLPRRQRSTYESDDDFCNDPE
                     ELESRQAQKKAARPKKERPSLFVRAIGSLARREYSRRDLEAKLRRSLSEDEDPDEVGR
                     VLDRLEGAGYLSDARFAESRARSRSRVMGDARIRRELRQCGVAGEHVDAAMDEIEEPE
                     EVRAYRLWARRFGELPADRKERDRQIRFMMYRGFGMSAISRVLKGAVEPPDDMA"
     gene            complement(2165..3280)
                     /gene="recA"
                     /locus_tag="ACZPB6_03895"
     CDS             complement(2165..3280)
                     /gene="recA"
                     /locus_tag="ACZPB6_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR1183650.1"
                     /translation="MAAKSANPTRPGRCEGEERKKALSVALANIEKQFGKGAIMRMAD
                     GAEVEDIETVSTGSLGLDLALGVGGLPRGRIIEVFGPESSGKTTLSLQVIAEMQKIGG
                     TCAFIDAEHALDVQYAQRLGVQLSDLLISQPDTGEQALEITDALVRSGSVDLIVVDSV
                     AALTPKSEIEGDMGDALPGLQARLMSQALRKLTGSITKTKSMVIFINQIRMKIGGYGN
                     PETTTGGNALKFYSSVRLDIRRIGAIKKGDEVIGNETRVKVVKNKVAPPFKQAEFDIL
                     YGEGISRYGEIIDIGTELNVLEKSGAWYSYNGQRLGQGKENVREFLKQHPDVCGEVEQ
                     KIREMKGIHLKSISAVDGDDEGMPEDDLPLADADIDL"
     gene            3670..3745
                     /locus_tag="ACZPB6_03900"
     tRNA            3670..3745
                     /locus_tag="ACZPB6_03900"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3703..3705,aa:Lys,seq:ttt)
     gene            3771..3846
                     /locus_tag="ACZPB6_03905"
     tRNA            3771..3846
                     /locus_tag="ACZPB6_03905"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3804..3806,aa:Lys,seq:ctt)
     gene            3879..3954
                     /locus_tag="ACZPB6_03910"
     tRNA            3879..3954
                     /locus_tag="ACZPB6_03910"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3912..3914,aa:Lys,seq:ctt)
     gene            4134..5183
                     /locus_tag="ACZPB6_03915"
     CDS             4134..5183
                     /locus_tag="ACZPB6_03915"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIR1183651.1"
                     /translation="MIKLDHITQTYKTPEGREFKALDDVSIEIRPGEIFGIIGRSGAG
                     KSTLVRCINLLNRPSEGTVTVDGKNLTELSEDELRESRRSIGMIFQHFNLLSSRTVYD
                     NVALPLELVGTPKNVIREKVEPLLKLVGLTEHAHKYPSQLSGGQKQRVGIARALTNDP
                     KVLLSDEATSALDPETTVATLALLKRINKELGLTIVMITHEMQVVKQICERVVVMNYG
                     KIVEQGKVVDIFMSPQHETTKALIGNVMARDMPASILDRFRKARENHPNSDAVYLLRL
                     AFSGNEVTRPVISECSRRFNLDFNILRGTVDDVQGQTLGSLTVLIEAENSVFIEAVNF
                     LRENGVVVEEINDVK"
     gene            5173..5826
                     /locus_tag="ACZPB6_03920"
     CDS             5173..5826
                     /locus_tag="ACZPB6_03920"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIR1183652.1"
                     /translation="MSSNLIQMIWDSFLETIIMVAISGGIGALAGIPLGILLFVTDRK
                     SFLPMPGFNAVLGTVINALRSVPFIILLVAIIPFTRLIVGSSIGTAAAIVPLTLSVAP
                     FIARIVETSLREVDKGLVEAAQSMGATNMQIITKVLLPEAMPGIVAGLTISIISLIGY
                     SAMAGAVGGGGLGDLGIRYGYQRFMPEVMWTVVIVLIILVQGIQSFGDWIVRRISHK"
     gene            6052..6852
                     /locus_tag="ACZPB6_03925"
     CDS             6052..6852
                     /locus_tag="ACZPB6_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1183653.1"
                     /translation="MKRRTLVAAAGILAIAGSIPLSALAAEKTIVVGVTAGPHAEIME
                     VVKRIAEKQGMKIRIVEFNDFVQPNAALVAGDLDINSFQHKPYMDQQNEDRGYKLASA
                     APTITFPLTYYTRKGYKTLADLPQGARVGIPNDPTNANRALHMLEEQGLVRLNPKAGI
                     RATVLDIIDNAKKLRIIELDSAQLPRSLEDLDAAAINASYAEPAGLTPAKDGIAQERP
                     DGPYANILCVRAEDLSKPWVAKFVQCYHSPEVKAFVEKRFNGGMICAW"
     gene            6912..7322
                     /locus_tag="ACZPB6_03930"
     CDS             6912..7322
                     /locus_tag="ACZPB6_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HIR1183654.1"
                     /translation="MDQNHNSETAPLSDAAVKFNSVLTFAQGFALVAIAVAALVGICS
                     SIWSMVELEKASLGDLLLLFLYIEILSMVKGSALGTREIPIRTPVALAIVAVARYIVV
                     DVEHMTPAFMLMTAGAILVLVCALWVLSHVRFRH"
     gene            7392..7465
                     /locus_tag="ACZPB6_03935"
     tRNA            7392..7465
                     /locus_tag="ACZPB6_03935"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7424..7426,aa:Gly,seq:ccc)
     gene            7551..8309
                     /gene="trmB"
                     /locus_tag="ACZPB6_03940"
     CDS             7551..8309
                     /gene="trmB"
                     /locus_tag="ACZPB6_03940"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR1183655.1"
                     /translation="MEKNCEKPELTPEELEALKKRHIRSFVMRRGHISAAQKRALDES
                     LPQYQIEYAPELLDADKAFGRHAPLVLEIGCGMGETTAAIAQAHPEVNFLGCEVFVAG
                     VGALAKRLDEMSLTNVRIVRHDAVEIVRDMIAENSLDGVHIYFPDPWRKARHHKRRLV
                     AQPFIGLLASRIRPGGYIHCATDWENYSEQMLEVLEGEPLLENLHGAGNFSPVMGNPL
                     CERPRTKFQARGERLGYGIWDLVYIRKQPAPSAS"
     gene            complement(8369..9199)
                     /locus_tag="ACZPB6_03945"
     CDS             complement(8369..9199)
                     /locus_tag="ACZPB6_03945"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR1183656.1"
                     /translation="MFDWLYLLQAAFLGIVEGLTEFLPVSSTGHLIVAADLVGFTDAT
                     SDTFVVAIQAGAILAVCWYYRKRIIRVLRGLFSEPVQQRLVVNTLVAFLPAAVVGVLV
                     SDLLKTYLFNPLTVATALVIGGLIILYVEHRHEKLGIEPRVKEMDDMDWKDALKVGVA
                     QCLAMIPGTSRSGATIIGGLIFGLSRKAATEFSFFLAIPTIFGATVWDLWQAREQLVA
                     ANLPGLAVGTVVSFFSALVVVHWLIRFVSRHDFRGFGWYRIIFGGVILLTWATGLVSW
                     "
     gene            9362..10057
                     /locus_tag="ACZPB6_03950"
     CDS             9362..10057
                     /locus_tag="ACZPB6_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA/YgfB family protein"
                     /protein_id="HIR1183657.1"
                     /translation="MANEKQMDSELIEELQQRLEALNNEGVMDAGVIDGFLTAYALNP
                     EHPSKHDIYPYIFSEEGDPAALPEDDRILELLDIRLNEIQAALAAKGGLDPVIFPLVD
                     DNDEIITDEEGIEALEPWASGFFFGMMHWREDLIADDEINSLATPIIRNISPDDFETP
                     EEAEQFVQEYRSGEMPKNLEDALYDLVKAVFDLKQLVDPNKPVVREEPRVGRNDPCPC
                     GSSKKFKQCCGKK"
BASE COUNT         2106 a         3401 c         2839 g         1881 t
ORIGIN      
        1 actatctgaa cgtgcccgag agaattcccg atttcttcgc tgcggttgac gaagacgaca
       61 actaggcgac cgagcagggc ctcgacgaca gtcatctgag cgaacaaggg cttcgtgcac
      121 gcgaagagcg cagggcaatg gaactgtctc aaaaggagct ggcggaaatc gagggcgtca
      181 cggccaaaca gatctgcagg cgcgagaccg aacgcctgac ttggaaggcg aagatgaagg
      241 aagagaagaa caaatgaggc ttgacctcct tccgacgctc gcctgaaaca aaaaaagcgc
      301 agcccttgcg ggctgcgctt ttttgggtac tgcatcagag ggaggcagct cagctgcgat
      361 ccttttccat ttccttcgca agcaacttct tcatcttcgc cttgccgagg aagtgaccga
      421 tgaggatcac gagcgccgca cagcccacgg agagcgggta gtgcatcatg aaggcctgtt
      481 cgggccagcg cgtcgtgacg aacacgtcgg aggcgatcat gccgccggca atccagccga
      541 gaaggcctgc gccgaagtag atgatggcgg ggaagcggtc gatgagcttc aggatgatct
      601 gggagccgaa gacgatgaac ggcaccgaga cgagcagccc gaagatgatc aggagcgtct
      661 gatgggtctc atgagcctgc tgggcggcgc ccgcaatggc gatcacgttg tcgaagctca
      721 tcacgaagtc cgcgacgatg atggtcttga tggcgccgat gagcttgctg ctgccctcga
      781 ccttttcatg atcgccgtcc tcgggaagca ggagcttggc gccgatccag acgagaagcg
      841 caccgcccgc caccttgagg aacgggaggt cgagaagcat caccgcgcag gtgatcagca
      901 cgacgcgcag ggcgatggcg ccggccgtgc cccagaagat gcccttgttg cgctggctct
      961 caggcaggtt gcggcacgcc atggcgatca cgacggcatt gtcgccgccg agaagaatgt
     1021 cgatcatgat gatctggccg accgcagccc aatgcaggtt ggcaagcaga tcgaagagat
     1081 attccaaaat agcctccgtg ggaaaatctc aaaaacaggt agccgatgat tttaacaaaa
     1141 gcaacaggaa aaaacttgaa atcccccgcc tcgaatccgc ttcaggacag ggatcgggag
     1201 cccgttcgaa caatgagccg gcaaacaaat cgctaggcca tgtcgtcagg cggctcgacg
     1261 gcgcccttga gcaccctgct gatggcgctc atgccgaatc cgcggtacat catgaagcgg
     1321 atctgcctgt cgcgctcctt cctgtccgca ggcagctctc caaagcgcct tgcccacagc
     1381 ctgtaggccc tgacctcctc gggctcctcg atctcgtcca tcgcagcatc gacatgctcg
     1441 cccgccacgc cgcactggcg cagctcccgc ctgatgcggg cgtctcccat gacccttgac
     1501 cttgagcggg cgcggctttc ggcaaagcgc gcgtcggaaa gatagcccgc gccctcgagc
     1561 ctatcgagca cgcgcccaac ctcgtccgga tcctcatcct ccgacaggga gcggcggagc
     1621 ttcgcctcaa gatcccgtct ggaatactcg cggcgggcca gcgagccgat cgccctgacg
     1681 aaaagactcg ggcgctcctt cttcggacgg gcggccttct tctgcgcctg ccggctctcg
     1741 agctcctcgg gatcgttgca gaagtcgtcg tcactttcat aggtgctccg ctggcggcgc
     1801 ggcagcacct tgggcgccgt gcgaacgatg cccgaggcct cgtcgacgcc cgtgacgatt
     1861 tcgccgagcc ccgagctgcg cccgacgcgc ctgacggcgg gctcttcgct gcggcgcacg
     1921 ggaggcgtct ccaaaggaag cgcacgtgcg ggttccgcgg cggaccgcac ggccttctct
     1981 tgcgaatcag cgggcacgag tccgtcgtca ccccagacga agagctcgcc ttcctcccag
     2041 tttcttccca tttcccttct cccacggatc attcacggaa aaaaagggga cggtcctcca
     2101 tgtccggacc accccccttt tccctgaagc cgttcttcaa tccggtccga aaaccggctc
     2161 tccattacag atctatgtcg gcgtcggcca gcggcagatc atcctcgggc atgccctcgt
     2221 catcaccgtc gacggcggag atgctcttga ggtggatgcc cttcatctcg cggatcttct
     2281 gctcgacctc gccgcagacg tcgggatgct gcttgaggaa ctcgcgcacg ttctccttgc
     2341 cctggccgag gcgctggccg ttgtaggagt accaggcgcc ggacttctca agcacgttga
     2401 gctcggtgcc aatgtcgatg atctcgccgt agcgggaaat gccctcgccg tagaggatgt
     2461 cgaactctgc ctgcttgaac ggaggcgcaa ccttgttctt gacgaccttg acgcgggttt
     2521 cgttgccgat cacctcgtcg cccttcttga tggcgccgat gcgacgaatg tcgagacgca
     2581 cggagctgta gaacttgagc gcgttgccgc ccgtcgtggt ttccgggttg ccgtagccgc
     2641 cgatcttcat gcggatctgg ttgatgaaga tcaccatgga cttcgtcttg gtgatcgagc
     2701 ccgtgagctt gcgcagggcc tgggacatca ggcgggcctg aaggccgggc agcgcgtcgc
     2761 ccatgtcgcc ctcaatttcg ctcttgggcg tcagcgcggc gaccgagtcc acgacaatca
     2821 ggtcgacgga gcccgagcgc acgagggcgt cggtgatctc aagggcctgt tcgcccgtgt
     2881 cgggctggga gatcagaagg tcggagagct gcacgccgag acgctgtgcg tactggacgt
     2941 cgagcgcgtg ttccgcgtcg atgaaggcgc acgtaccgcc gatcttctgc atttcggcga
     3001 tgacctgcag ggagagcgtc gtcttgccgg aggattcggg accgaacacc tcgatgatgc
     3061 gaccgcgggg cagaccgccc acgccgagcg caaggtcaag gcccagggag cccgtggaca
     3121 ccgtctcgat gtcctcgact tcggcgccgt cggccatgcg catgatggcg cccttgccga
     3181 actgcttttc aatattggca agcgcgacgc tgagcgcctt cttgcgttct tccccttcgc
     3241 aacgaccggg gcgggtcgga ttcgcggact tggcagccat gacggactcc tgtctcaaac
     3301 aatcacaata ctaaatattt atgaatacac gacaatcaat ttacatctcg aatggcagga
     3361 tcatttcact agcctcgatg cacaaaggat catccgcttc acctgcaaga cgggagtttt
     3421 gagcaccctg ccgccttgtt cgttaccgtc atgatgcctg aaaaaatccc gcttgccaag
     3481 gtctactttg ttggtgttta ccctaacaaa agacaccttt acattctatt tacgggcgcg
     3541 gaatgattgc cttaggcgac aggctcacca actttttttt catgcactat tgacaggtcc
     3601 ggaaggcacg tgcataattc gtttcctcag ttagttgttc acgtcgtttg tgactcatct
     3661 gcggcaaacg ggtcgttagc tcagttggta gagcagcgga cttttaatcc gttggtcgtg
     3721 ggttcgagtc ccgcacgacc caccacaact atctgcggac aatctggaat gggaacgtag
     3781 ctcagttggt agagcagcgg actcttaatc cgtcggtcca gggttcgaat ccctgcgttc
     3841 ccaccattcc ttctctgtcc gatacaatct gcttgtgtgg gaacgtagct cagttggtag
     3901 agcagcggac tcttaatccg tcggtccagg gttcgaatcc ctgcgttccc accacacctc
     3961 ccaatgatcc tgctgacaga tggtccgcgg gatttttttt tcgttcctct cttcccttcc
     4021 ggcggtaata cctcaagcat ccgcccattt ccccgcgctt tcgctccctt tgcgccgcac
     4081 aacaggtaaa ctgtccgaac cttccccttt gcgattcata cgcacataga aaaatgatca
     4141 agcttgacca cataacccag acctacaaga cgcctgaagg tcgggagttc aaggccctcg
     4201 acgacgtgtc gatcgagatc cgccccggcg agatcttcgg catcatcggc cgctccggcg
     4261 ccggcaagag cacgctcgtg cgctgcatca accttctcaa ccgcccgagc gaaggcaccg
     4321 tcacggtcga cggcaagaac ctcaccgagc tctccgaaga cgagctgcgc gaaagccgcc
     4381 gcagcatcgg catgatcttc cagcacttca acctgctgag ctcccgcacg gtctacgaca
     4441 acgtcgcgct cccgctcgag ctcgtcggca cgcccaagaa cgtcatccgc gaaaaggtcg
     4501 agccgcttct caagctcgtc ggcctcaccg aacacgccca caagtatccg tcccagctct
     4561 ccggcggcca aaagcagcgc gtcggcatcg cccgcgccct gaccaacgac cccaaggtcc
     4621 ttctctccga cgaggccacc tcggccctcg accccgagac gactgtcgcc accctcgcgc
     4681 ttctcaagcg catcaacaag gagctcggcc tcacgatcgt catgatcaca catgaaatgc
     4741 aggtcgtgaa gcagatctgc gagcgcgtcg tcgtgatgaa ctacggcaag atcgtcgagc
     4801 agggcaaggt cgtcgacatc ttcatgtcgc cgcagcacga aaccacgaag gccctgatcg
     4861 gcaacgtgat ggcccgcgac atgccggcct ccattctcga ccgcttccgc aaggctcgcg
     4921 aaaaccatcc caacagcgac gcagtctacc tgcttcgcct cgccttctcg ggcaacgaag
     4981 tcacgcgccc cgtcatttcc gaatgctcgc gccgcttcaa cctcgacttc aacatcctcc
     5041 gcggcactgt cgacgacgtc cagggccaga ccctcggctc gctcaccgta ctcatcgagg
     5101 ccgaaaacag cgtattcatc gaagccgtca acttcctgag ggagaacggc gtggtcgttg
     5161 aggagatcaa tgatgtcaag taatctcatt cagatgatct gggactcctt cctggagacg
     5221 atcatcatgg tcgccatctc cggcggcatc ggcgccctcg cgggcattcc cctcggcatc
     5281 ctgctcttcg tgacggaccg caagtccttc cttccgatgc cgggcttcaa cgccgttctc
     5341 ggcacggtga tcaacgctct ccgctcggtg ccgttcatca tcctgctcgt ggcgatcatt
     5401 ccgttcaccc gactcatcgt cggctcctcg atcggcacgg ccgccgccat cgttcctctg
     5461 acactctcgg tggcgccctt catcgcccga atcgtcgaga cgagcctgcg cgaagtggac
     5521 aagggcctcg tcgaggccgc ccagtcgatg ggtgccacga atatgcagat catcacgaag
     5581 gtcctgcttc ccgaagccat gcccggcatc gtcgcgggcc tgacgatctc gatcatcagc
     5641 ctgatcggct actccgccat ggccggcgcc gtgggcggcg gcggtctcgg cgacctcggc
     5701 atccgctacg gctaccagcg cttcatgcct gaagtcatgt ggaccgtcgt gatcgtgctg
     5761 atcatcctcg ttcagggcat ccagagcttc ggcgactgga tcgtgcgaag gatctcccac
     5821 aagtaagctc agcaggacac tgcagaaggc gcgctgctcg aaagcggcgc gctttttttc
     5881 aatccctcgt gttccatctc cctcaaaccg ggcggaacat caggtgtttt ccgtatgccg
     5941 gtgtgcggta atcaacatac cttcaatctc cccgacggcc ccctgcaccc gcgtgcataa
     6001 tgccgccacg ccccgcctcg gggcggttca tacccgaaaa ggacattccg aatgaaacgc
     6061 cgcacccttg tcgccgccgc aggcattctt gccattgccg gcagcattcc gctctccgcg
     6121 ctcgccgccg aaaagaccat cgtcgtcggc gtcaccgcag gcccgcacgc cgaaatcatg
     6181 gaagtcgtca agaggatcgc cgaaaaacag ggcatgaaga tccgcatcgt cgaattcaac
     6241 gacttcgtgc agcccaacgc ggcgctcgtc gcgggcgatc tggacatcaa ctcgttccag
     6301 cacaagccct acatggacca gcagaacgag gaccgcggct acaagctcgc ctccgccgcc
     6361 cccaccatca cgttcccgct cacctactac acccgcaagg gctacaagac ccttgcggac
     6421 cttccccagg gcgcccgcgt cggcattccg aacgatccga cgaacgccaa ccgcgccctg
     6481 cacatgctcg aggaacaggg actcgtgaga ctcaatccga aggccggcat tcgcgccacc
     6541 gtcctcgaca tcatcgataa cgccaagaag ctccgcatca tcgagctcga ctccgcccag
     6601 ctgccccgca gcctcgagga ccttgacgcc gccgccatca acgcgagcta cgccgagccc
     6661 gcaggactca cgcccgccaa ggacggcatc gcccaagaac gccccgacgg cccctacgcc
     6721 aacatcctct gcgtccgcgc cgaggacctc tccaagccct gggtcgccaa gttcgtccag
     6781 tgctaccact cccccgaagt caaggcattt gtagaaaagc gcttcaacgg gggcatgatc
     6841 tgcgcctggt gatgctaata tgcaatgaac aaacacaccc tcgtttttca taagcattac
     6901 ctatcgccac aatggatcaa aaccacaact ccgagacggc gccgctttcc gatgccgccg
     6961 tcaagttcaa ctctgttctg acgttcgctc agggcttcgc gctcgttgcc atcgccgtcg
     7021 ccgccctggt cggcatctgc tcctcgatct ggagcatggt cgagcttgag aaggccagcc
     7081 tcggcgacct gctcctgctc tttctctaca tcgagatcct ctccatggtg aagggctccg
     7141 cgctcggcac ccgcgagatc ccgatccgca cgccggtggc tcttgccatc gtggccgtcg
     7201 cccgctacat cgtggtcgac gtcgagcaca tgacgcccgc cttcatgctc atgaccgcag
     7261 gcgccattct ggttctcgtc tgcgccctct gggtgctcag ccacgtgcgc ttccgccact
     7321 gagcggcaac gtccggcgca tgccgcccga agaacggcgg catgctttta tggtagaatt
     7381 ccagacctgt tgcgggtgta gctcaatggc agagcaatag cctcccatgc taaagacgag
     7441 ggttcgattc ccttcacccg ctccatattc caaggatccc gcctgagcgt aagctgcggg
     7501 atcttttcgt atctaccgcc gccccgtgcg gcccgcccct gctccgcacc atggaaaaga
     7561 actgcgaaaa gcccgagttg acgcctgaag aactcgaggc gctcaagaag cgccacatcc
     7621 gctccttcgt catgcgccgc ggccacatct cggccgccca gaagcgcgcc cttgacgagt
     7681 cgctcccgca ataccagatc gagtacgccc ccgagcttct cgacgccgac aaggccttcg
     7741 gccgccacgc cccgctcgtt ctcgaaatcg gctgcggcat gggcgagacg accgccgcca
     7801 tcgcgcaggc ccaccccgag gtcaacttcc tcggctgcga ggtcttcgtg gccggcgtcg
     7861 gcgcgcttgc caagcgtctc gacgagatgt ccctcaccaa tgtgcgcatc gtccgccacg
     7921 acgccgttga aatcgtgcgc gacatgatcg ccgagaactc cctcgacggc gtgcacatct
     7981 acttccccga tccctggcgc aaggcccgcc atcacaagcg acgcctcgtc gcccaaccct
     8041 tcatcggact cctcgcctcc cgcattcgtc cgggcggcta catccactgc gccacggact
     8101 gggagaacta ctccgagcag atgctcgaag tgctcgaggg cgaaccgctc ctcgagaacc
     8161 tccacggcgc cggcaacttc agccccgtga tgggcaaccc gctctgcgag cgtccccgca
     8221 cgaagttcca ggcccgcggc gaacgcctcg gctacggcat ctgggatctg gtctacatcc
     8281 gcaagcaacc cgctccctct gcgtcataaa aaaagcgcgc cttcccggaa aaggcgcgct
     8341 tttttcttga gccatcggca aacggcgatc accagctcac cagccccgtc gcccaggtaa
     8401 ggaggatcac gccgccgaag atgatgcggt accatccgaa gccgcggaag tcgtgcctcg
     8461 aaacaaagcg gatgagccag tggacgacga cgagcgcaga gaagaaggac accaccgttc
     8521 cgaccgccag tcccggcaga ttggcggcga caagctgctc gcgcgcctgc cagaggtccc
     8581 agaccgtggc gccgaagatc gtcggaatcg cgaggaagaa ggagaactcg gtcgcagcct
     8641 tgcgcgaaag cccgaagatg aggccgccga tgatcgtggc gcccgagcgg ctcgtgccgg
     8701 gaatcatcgc gagacactgc gcgacgccga ccttgagcgc gtccttccag tccatgtcgt
     8761 ccatctcctt cacgcgcggc tcgatgccga gcttctcatg acgatgctcg acatagagga
     8821 tgatgaggcc gccgatgacc agcgccgtcg ccacggtgag cggattgaag agataggtct
     8881 tgagaagatc cgagacaaga acgccgacga cggcagcggg gaggaaggcc acgagcgtat
     8941 tgacgacgag gcgctgctgg acgggctccg aaaagagacc acggaggacc ctgatgatcc
     9001 tcttcctgta gtaccaacag accgcaagga tcgcacccgc ctgaatggcg acgacgaacg
     9061 tgtctgacgt tgcatccgtg aatcccacga ggtccgccgc cacgatcaga tgcccagtcg
     9121 aactcaccgg aaggaattca gtgaggcctt cgacgatgcc gaggaaggcg gcctgcagca
     9181 gataaagcca gtcgaacatt gagcgtcctg tatcggaatg gtgaaaagcg cccatattgt
     9241 cgcagatacg acgcacggac acacttgagg gaaacaagcc gttacggctc tcgagccctg
     9301 caggccaaag cccgctttgt taagatagcg ccatcatcaa tcctcaaccg gaactccaga
     9361 aatggcgaac gaaaaacaga tggacagcga gcttattgaa gagcttcagc agcgccttga
     9421 agcgctcaac aacgaaggcg tgatggatgc aggcgtgatc gacggcttcc tgaccgccta
     9481 tgcgctcaac cccgagcacc cgagcaagca cgacatctac ccctacatct tcagcgagga
     9541 aggcgatccc gccgccctgc ccgaagacga ccgcatcctc gagctgctcg acattcgcct
     9601 caacgaaatc caggccgcgc tggccgccaa gggcggtctt gatccggtca tcttcccgct
     9661 cgtcgacgac aacgacgaga tcatcaccga cgaagagggc atcgaagccc tcgaaccctg
     9721 ggcctccggc ttcttcttcg gcatgatgca ctggcgcgaa gacctcatcg ccgacgacga
     9781 gatcaactcg ctcgccacgc cgatcatccg caacatctct cccgacgact tcgaaacccc
     9841 cgaggaagcc gagcagttcg tgcaggaata ccgcagcggc gagatgccga agaacctcga
     9901 ggacgccctc tacgacctcg tcaaggccgt cttcgatctc aagcagctcg tcgatccgaa
     9961 caagcccgtc gtgcgcgaag aaccccgcgt aggccgcaac gacccctgcc cctgcggcag
    10021 cagcaagaag ttcaagcagt gctgcggtaa aaagtaaggt tcatctcgga agtccgtgga
    10081 acgcggcctt gacttttaag aaaaagtctg gtcggctccc tccaagaaaa aagtagcgct
    10141 cactccaaat ttcaggcaga ttcaagttac tagagaagaa ccgcgaaaaa aggatgagcg
    10201 ctatgaccaa tattgtaggg caacttt
//
LOCUS       DBMDTI010000049        10140 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1921_length_10140_cov_4.265963, whole genome shotgun sequence.
ACCESSION   DBMDTI010000049 DBMDTI010000000
VERSION     DBMDTI010000049.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1921_length_10140_cov_4.265963"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2238
                     /gene="rpoB"
                     /locus_tag="ACZPB6_03955"
     CDS             <1..2238
                     /gene="rpoB"
                     /locus_tag="ACZPB6_03955"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR1183658.1"
                     /translation="AQANAEMDENGRLTQELVSARDNGETVLASPDRIQYMDVAPSQI
                     VSCAAALIPFLEHDDANRALMGSNMQRQGVPCLRPEKPVVGTGIERTVAVDSKTAVQA
                     RRGGVVDYVDANRVVIRVNDEESIAGETGVDIYNLQKFTRSNQSTNINQRPIVVKGDR
                     VAVGDVLADGASTDTGELALGQNMLIAFMPWNGYNYEDSVLISERVVADDRYTSIHIE
                     ELSVVARDTKLGAEEITRDISNLSENQLSRLDDSGIVFIGAEVKAGDVLVGKVTPKGE
                     TQLTPEEKLLRVIFGEKASDVKDTSLRVPSGMTGTVIDVQVFTRDGVKRDKRAESIIE
                     DALKRYRRDLDDQLRIVERDAFDRLRRQLVGHKVAGGPDAFKPGVALTMEMLEAVPGY
                     DLFNLRMEEEGAQHIIDLTMRAIQDTREQNDRKYATKKDKLTRGDELPPGVLKMVKVY
                     IAERRRLQPGDKMAGRHGNKGVVSKICPVEDMPYMADGRPADIVLNPLGVPSRMNIGQ
                     VLEVHLGWAAKGLGWRIEEMLKTEQVRQIRAFLNEVYNRTGKKEDLDSLTDDELMAMA
                     RNLRNGVPFATPVFDGATEEQVRMMLDLAFPDDEAARLQLTPAKTQARLYDGRTGEAF
                     ERPVTVGFMHYLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALE
                     AYGASYVLQEMLTVKSDDVNGRTKVYENIVKGDHKIEAGMPESFNVLVKEIRSLGIDI
                     DLVKN"
     gene            2253..6566
                     /gene="rpoC"
                     /locus_tag="ACZPB6_03960"
     CDS             2253..6566
                     /gene="rpoC"
                     /locus_tag="ACZPB6_03960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR1183659.1"
                     /translation="MRMPLNDIFNQVQSEDHFDAIRIGLASPEKIHSWSYGEVKKPET
                     INYRTQKPERDGLFCAKIFGPVKDYECLCGKYKRLKNRGVICEKCGVEVTLAKVRRER
                     MGHIDLASPVAHIWFLKSLPSRMGMVLDIPLRDIERVLYFEAYIVVDPGLSTLERGQL
                     LSEEDFIAATTEFGDDFVAMMGAEAIGELLRTIDIDQEVERLRTELRETSSEAKIKKF
                     AKRLKVLEGFQRSGIKPEWMIMTVLPVLPPDLRPLVALDGGRFATSDLNDLYRRVINR
                     NNRLKRLLEIKAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIKG
                     KSGRFRQNLLGKRVDYSGRSVITVGPELRLHQCGLPKLMALELFKPFIFNKLVLRGLA
                     VTPKAAKKMVENQDPVVWDILEEVIYEHPVMLNRAPTLHRLGIQAFEPKLIEGKALQL
                     HPLVCAAFNADFDGDQMAVHVPLSLEAQLEARSLMMASNNVLFPANGDPSIVPSQDMV
                     LGLYYATREKINGKGEGMFFADVAEVQRAWDNKVVELQTRCTVRIREYDVNKETGEKT
                     PRMVRYETTVGRALLSEILPEGMSFKELNITLKKKEIAKLINRCFRKCGLRATVMFAD
                     KLKDNGYRLSTRAGISICVGDMAVPAQKEKLVHDAEQEVKEIEAQYTSGLVTQGERYN
                     KVVDIWGRTADQVGKVMMTELSTEPTIDRHGKKVRQESFNSVYMMADSGARGSAAQIR
                     QLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVTEDDCGTTNGVEMRALVEGGEVIQALRDRVLGRVTATDVVNPDDG
                     EVVIAAGTLIDEDCCDLIEKLAIDMVKVRTPLTCETRYGLCAKCYGRDLGRGSIVNAG
                     ESVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAVASSVEAKSSGVVSYSPAMRYVRSA
                     KGELVVISRSGEIVITENGRERERHKVPYGATLLVMPDQEIKAGQMLANWDPMTRPII
                     TEYAGTIKFENVVENVTVMNQVDEVTGLSSLVVIDPKRSTTTAKGKGKSQNAGILRPL
                     VHLIDENGNEVKIPGTDHAVTIQLPVGAFVVVRDGQQIGMGEVLARIPVESQKTRDIT
                     GGLPRVAELFEARSPKDAGMLAEVTGTVTFGKETKGKQRLIITGNDGVAHETLISKDK
                     TVLVHDGQVVQKGEEVVDGPVDPHDILRLLGIEELARYIVDEVQDVYRLQGVKINDKH
                     IEVIVRQMLRRVQITDPGDTHFITGEQVERSEMLDENDRVVAQGLRPASYDNVLLGIT
                     KASLSTDSFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLAYHQA
                     HKAREQAERLEAETARASVTTEDPFLESSAVVDPFASLDAPLTTTGEEFADGSKKEGA
                     K"
     gene            6822..7199
                     /gene="rpsL"
                     /locus_tag="ACZPB6_03965"
     CDS             6822..7199
                     /gene="rpsL"
                     /locus_tag="ACZPB6_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR1183660.1"
                     /translation="MPTINQLVRKPRELTHDKSKSPALKNCPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGSL
                     DTQGVKDRKQARSKYGAKRPKAA"
     gene            7358..7828
                     /gene="rpsG"
                     /locus_tag="ACZPB6_03970"
     CDS             7358..7828
                     /gene="rpsG"
                     /locus_tag="ACZPB6_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR1183661.1"
                     /translation="MPRRREVPKREVLADPKFGSVEITKFINVIMLDGKKAVAERIVY
                     GALQQIEEKSGKNALEVFTTALNNVRPLVEVKSRRVGGANYQVPVEVRPVRRMALAMR
                     WLRESAKKRSEKSMPQRLAGELLDAAEGRGGAMKKRDEVHRMAEANKAFSHFRF"
     gene            7889..9991
                     /gene="fusA"
                     /locus_tag="ACZPB6_03975"
     CDS             7889..9991
                     /gene="fusA"
                     /locus_tag="ACZPB6_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR1183662.1"
                     /translation="MARQTPISRYRNIGISAHIDAGKTTTSERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWRGMEMQWEPYRLNIIDTPGHVDFTVEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQANKYRVPRLAFVNKMDRTGANFFKVYDQMKK
                     RLNANPVPVVVPIGAEDQFEGVVDLIKMKGIIWDAKSQGMTFEYVDIPANLVDVAQEW
                     HEKMVESAAEASEELMNKYLENGELSEEDIIAGLRQRTIACEIQPMLCGSAFKNKGVQ
                     RMLDAVVQFLPAPSDIPPVEGFDLDEKPTFREAKDEAPFAALAFKIMTDPYVGQLTFL
                     RVYSGLLNSGDTVMNSVKGQKERIGRLLQMHANERKEIKFVEAGDIAAAVGLKNVTTG
                     DTLCAIDAPVILERMEFPEPVISQAVEPKTKADQEKMALALNRLAQEDPSFRVRTDEE
                     SGQTIISGMGELHLEILVDRMKREFNVEASVGKPQVAYRETIRSVVENAECKFAKQSG
                     GRGQYGHVVLKLEPLEPGKGFEFVDAIKGGVVPREYIPAVEKGVEDTLKAGVLAGYPV
                     VDVKVTLHFGSYHEVDSNENAFKMAASMAFKEGMRKADPVLLEPIMAVEVETPEEKMG
                     DVMGDLSSRRGVIQGMDDLVGGGKSIKAEVPLAEMFGYATQLRSLTQGRATYTMEFKH
                     YAEAPRNVAEAVIADKAK"
     gene            10040..>10140
                     /locus_tag="ACZPB6_03980"
     CDS             10040..>10140
                     /locus_tag="ACZPB6_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1183663.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITT"
BASE COUNT         2167 a         3166 c         2897 g         1910 t
ORIGIN      
        1 gctcaggcca acgccgagat ggacgagaac ggccgtctga cgcaggaact cgtttccgct
       61 cgtgacaacg gcgaaacggt tctcgccagc ccggaccgca tccagtacat ggacgtggct
      121 ccgtctcaga tcgtctcctg cgccgccgct ttgattccgt tcctggaaca cgacgacgcg
      181 aaccgtgctc tgatgggttc gaacatgcag cgtcagggcg tgccctgcct ccgtccggaa
      241 aagccggtcg tgggtaccgg catcgagcgc acggttgccg ttgactccaa gactgccgtt
      301 caggctcgcc gcggcggcgt cgtcgactat gtcgacgcga accgcgtggt gatccgtgtg
      361 aacgacgagg aaagcatcgc cggcgaaacc ggtgtggaca tctacaacct gcagaagttc
      421 acgcgttcca accagtccac gaacatcaac cagcgtccga tcgttgttaa gggtgaccgg
      481 gttgcggtcg gcgacgtgct cgccgacggt gcctccactg acaccggcga actcgccctc
      541 ggccagaaca tgctgatcgc gttcatgccg tggaacggct acaactacga agactcggtg
      601 ctcatctccg aacgcgtggt tgccgacgat cgctacacct cgatccacat cgaggagctc
      661 tccgtcgtcg cccgcgacac gaagctcggc gctgaggaaa tcacgcgcga catctcgaac
      721 ctgtccgaaa accagctgtc ccgtctcgac gactctggca tcgtgttcat cggtgctgaa
      781 gtgaaggccg gcgacgtgct tgtcggcaag gtcacgccga agggcgaaac ccagctcacg
      841 ccggaagaaa agctcctgcg cgtgatcttc ggtgagaagg cctccgacgt caaggatacc
      901 tcgctgcgcg ttccgtccgg catgaccggc accgtgatcg acgttcaagt cttcacgcgt
      961 gacggcgtta agcgcgacaa gcgtgccgaa agcatcatcg aggacgccct caagcgctat
     1021 cgccgcgacc tggatgacca gctgcgcatt gttgaacgcg acgcgttcga ccgtctgcgt
     1081 cgtcagctcg tcggccacaa ggttgccggc ggccccgacg ccttcaagcc gggcgttgcg
     1141 ctcacgatgg aaatgctcga ggccgttccc ggctacgatc tcttcaacct ccgcatggag
     1201 gaagagggtg cccagcacat catcgatctg acgatgcgcg ccattcagga cacgcgtgaa
     1261 cagaacgacc gcaagtacgc cacgaagaag gacaagctca cccgcggcga cgaactgccc
     1321 ccgggcgtgc tcaagatggt caaggtgtac atcgccgaac gccgtcgtct gcagcctggc
     1381 gacaagatgg ccggccgtca cggtaacaag ggtgtggtct ccaagatctg cccggttgaa
     1441 gacatgccgt acatggccga cggtcgtccc gccgacatcg ttctgaaccc gctcggcgtg
     1501 ccgtcccgta tgaacatcgg tcaggtgctt gaagtgcatc tcggctgggc tgccaagggt
     1561 ctcggctggc gcattgagga aatgctcaag accgaacagg tccgccagat ccgcgccttc
     1621 ctgaacgagg tgtacaaccg cacgggcaag aaggaagatc tcgacagcct taccgacgac
     1681 gagctgatgg ccatggcgcg caatctgcgc aatggcgttc cgtttgccac gccggtgttc
     1741 gacggtgcca cggaagaaca ggtccgcatg atgctcgacc tcgccttccc ggacgacgaa
     1801 gccgcccgtc tgcagctcac gcccgccaag acgcaggccc gcctctatga cggccgcacc
     1861 ggtgaagcgt tcgagcgccc cgtcacggtt ggcttcatgc actatctgaa gctgcaccac
     1921 ctggtcgacg acaagatgca tgcccgttcc acggggccgt actccctcgt cacccagcag
     1981 ccgctcggcg gcaaggcgca gttcggcggc cagcgcttcg gtgaaatgga agtctgggcc
     2041 ctcgaagcct acggcgcctc ctacgtgctg caggaaatgc ttactgtcaa gtctgacgac
     2101 gttaacggtc gtaccaaggt ttacgaaaac atcgtcaagg gtgaccacaa gattgaagcg
     2161 ggcatgcccg aatccttcaa cgtgttggtc aaggaaatcc ggtcgttggg tatcgacatc
     2221 gacctcgtca agaactaagg ggaaattaag cgatgagaat gccgcttaac gatatcttca
     2281 accaggtcca gtcggaagat catttcgacg cgatccgcat cggacttgcc agtcccgaaa
     2341 agatccactc ctggtcctac ggtgaagtca agaagcctga gacgattaac tatcgtaccc
     2401 agaagccgga acgcgacggt cttttctgtg ccaagatctt tggcccggtg aaggattatg
     2461 aatgcctttg cggcaagtac aagcgcctca agaatcgcgg cgtgatctgc gaaaagtgtg
     2521 gcgttgaagt cacgctcgcc aaggttcgcc gcgaacgcat gggtcacatc gacctcgcca
     2581 gccctgtcgc tcacatctgg ttcctcaaga gccttccgag ccgtatgggc atggtgctcg
     2641 acatcccgct gcgcgacatc gagcgcgtgc tgtacttcga agcctacatc gtcgttgatc
     2701 cgggcctttc cacgctcgag cgtggccagc tgctcagcga agaggacttc attgccgcca
     2761 cgaccgaatt cggtgacgac ttcgtcgcca tgatgggcgc tgaagccatc ggcgagctcc
     2821 tgcgcacgat cgacatcgat caggaagtcg aacgtctgcg caccgagctc cgcgagacga
     2881 gctccgaagc caagatcaag aagttcgcca agcgcctcaa ggtgcttgaa ggcttccagc
     2941 gctccggcat caagcccgaa tggatgatca tgacggtcct cccggttctt ccgccggacc
     3001 tgcgtccgct cgtcgccctt gacggcggcc gcttcgcgac gtccgacctg aacgacctgt
     3061 accgccgcgt catcaaccgt aacaaccgtc tgaagcgcct cctcgagatc aaggctccgg
     3121 acatcatcgt gcgcaacgaa aagcgcatgt tgcaggaagc cgtcgactct ctgctcgaca
     3181 acggccgccg cggcaaggcg atgaccggtg ccaacaagcg cccgctcaag tccctcgccg
     3241 acatgatcaa gggcaagagc ggccgcttcc gtcagaacct gctcggcaag cgcgtggact
     3301 actccggccg ttccgttatt acggtgggtc ctgaactccg tctgcaccag tgcggtctgc
     3361 cgaagctcat ggctctcgag ctcttcaagc cgttcatctt caacaagctc gtgctccgcg
     3421 gtctcgccgt gacgccgaag gctgccaaga agatggttga gaaccaggat cctgtcgtct
     3481 gggacatcct cgaagaggtg atctacgaac acccggtcat gctcaaccgt gctccgacgc
     3541 tgcaccgtct cggcattcag gcgttcgagc ccaagctgat cgaaggcaag gccctccagc
     3601 tgcatccgct cgtctgcgcc gccttcaatg ccgacttcga cggtgaccag atggccgttc
     3661 acgtgccgct ctcgctcgaa gcccagcttg aagcccgctc gctgatgatg gcctccaaca
     3721 acgtcctgtt cccggccaac ggcgatccgt cgatcgtgcc gtctcaggac atggtgctcg
     3781 gtctctacta cgctacgcgt gaaaagatca acggcaaggg cgagggcatg ttctttgccg
     3841 acgtcgccga agttcagcgc gcctgggaca acaaggttgt cgaactccag acccgctgca
     3901 cggtccgtat ccgcgaatac gacgtcaaca aggaaacggg tgaaaagacc ccgcgcatgg
     3961 ttcgttacga aaccaccgtc ggccgcgctc tcctctccga aatcctgccg gaaggcatga
     4021 gcttcaagga actcaacatc accttgaaga agaaggagat cgccaagctg atcaaccgct
     4081 gcttccgcaa gtgcggcctg cgcgcgacgg tcatgttcgc cgacaagctc aaggacaacg
     4141 gctaccgtct gtcgacgcgc gccggcatct ccatctgcgt gggtgacatg gccgtccccg
     4201 ctcagaagga aaagctcgtc catgacgccg aacaggaagt caaggaaatc gaagcccagt
     4261 acacgtccgg tctcgtgacc cagggtgaac gctacaacaa ggtggtggac atctggggcc
     4321 gcacggccga ccaggtgggc aaggtgatga tgaccgagct ctccaccgaa ccgacgatcg
     4381 accgtcacgg caagaaggtt cgtcaggaat cgttcaacag cgtctacatg atggccgact
     4441 ccggtgctcg tggttccgca gcccagattc gccagctcgc cggcatgcgc ggcctgatgg
     4501 ccaagccgga cggctccatt attgaaacgc caattaccgc gaacttccgt gaaggcttga
     4561 acgttctgca gtacttcatt tcgacccacg gtgcccgtaa gggtctggcc gacacggctc
     4621 tgaagaccgc taactccggt tacctgacgc gtcgtctcgt cgatgtgacg caggacctcg
     4681 tggtgacgga agacgactgc ggcacgacca acggcgttga aatgcgcgct ctggtcgaag
     4741 gcggtgaagt cattcaggct ctccgtgacc gcgtccttgg ccgcgtgacc gccaccgacg
     4801 tcgtcaaccc cgacgacggc gaagtggtca ttgccgccgg cacgctgatc gacgaagact
     4861 gctgcgatct gatcgaaaag ctcgcgatcg acatggtcaa ggtccgtacg ccgctcacct
     4921 gcgaaacgcg ctacggcctt tgcgccaagt gctacggccg cgacctcggc cgcggctcga
     4981 tcgtcaatgc cggtgaatcc gtcggcgtga tcgccgccca gtccatcggt gaaccgggta
     5041 cccagctgac gatgcgtacg ttccatatcg gtggtgccgc ttcccgtgcc gccgtggcct
     5101 ccagcgtcga agccaagtcc tccggtgtcg tgagctactc cccggccatg cgctacgtcc
     5161 gctccgccaa gggcgaactg gtcgtcattt cccgttctgg cgaaatcgtc attacggaaa
     5221 acggccgcga acgcgaacgc cacaaggttc cgtacggcgc cacgttgctc gtgatgcccg
     5281 accaggaaat caaggccggc cagatgctcg ccaactggga tccgatgacg cgtccgatta
     5341 tcacggaata cgccggtacg attaagttcg aaaacgtcgt tgaaaacgtg accgtgatga
     5401 atcaggtcga cgaagtgacg ggcctttcca gcctcgtcgt cattgacccg aagcgctcca
     5461 ccacgaccgc caagggcaag ggcaagtccc agaacgccgg catcctgcgt ccgttggttc
     5521 acctcattga cgaaaacggc aatgaagtga agatcccggg cacggaccat gccgtgacga
     5581 ttcagctgcc tgtcggcgcc ttcgtggttg tccgtgacgg ccagcagatc ggcatgggtg
     5641 aggttctcgc ccgcatcccg gtcgaatcgc agaagacccg agacattacc ggcggtctgc
     5701 cgcgcgttgc cgaactcttt gaagcccgca gcccgaagga tgccggcatg ctcgccgaag
     5761 tgacgggcac ggtgaccttt ggcaaggaaa cgaagggcaa gcagcgcctg atcatcaccg
     5821 gcaacgacgg cgtcgcccac gaaacgctga tcagcaagga caagacggtt cttgtccatg
     5881 acggtcaggt cgttcagaag ggtgaagaag tggttgacgg tccggtcgat ccgcacgaca
     5941 tcctccgcct gctcggcatc gaggagctcg cccgctacat cgtcgacgaa gtgcaggacg
     6001 tttatcgtct gcagggtgtg aagatcaacg acaagcacat tgaagtgatc gtccgccaga
     6061 tgctgcgccg cgtccagatc accgatccgg gcgacacgca cttcatcacc ggcgaacagg
     6121 tcgaacgctc cgaaatgctc gacgagaacg atcgcgtcgt cgctcagggc ctgcgccccg
     6181 ccagctacga caacgtcctc ctcggcatta cgaaggcctc gctttccacg gacagcttca
     6241 tctcggccgc ttcgttccag gaaacgacgc gcgtccttac ggaagccgcc atcatgggca
     6301 agcaggacga gctccgcggt ctgaaggaaa acgtcatcgt cggccgtctg atccccgccg
     6361 gtacgggtct cgcctatcat caggctcaca aggctcgcga acaggccgaa cgcctggaag
     6421 ctgagaccgc ccgcgcttcg gtcacgaccg aggatccgtt cctcgagtcc tccgccgttg
     6481 tcgatccgtt cgcatctctt gatgcgccgc tgacgaccac tggcgaagag tttgccgacg
     6541 gttccaagaa ggaaggcgcc aagtaatagc gcccgagttc tgaaatgaac agaaagcccg
     6601 tctcctgaaa gggaggcggg ctttttagta caaaaaaagc cccttcactt gacagggaag
     6661 ggtgttatgg tcgaaaatca catgcttttc agggacagtg tctccatcga atcggctctt
     6721 tgcctctgcg cctaggctgt tcggggagaa tagggtgcct gaagcgttta taaccagagc
     6781 ctgcatggtg caggccaaac tttagtttta gacggacgtt tatgcctact attaaccagc
     6841 ttgttcgcaa gccccgtgag ctcacgcacg acaagagcaa gagccccgct cttaagaact
     6901 gcccgcagaa gcgcggcgtt tgcactcgtg tgtacaccac gaccccgaag aagccgaact
     6961 ctgctcttcg taaggtcgcc aaggtgcgcc tgaccaacgg tttcgaagtc atttcgtaca
     7021 tcggcggtga aggccacaac ctccaggaac actctgtcgt gcttatccgc ggcggccgtg
     7081 tgaaggacct tcctggtgtt cgttaccaca tcgtccgcgg ctctctggat acccagggcg
     7141 tcaaggatcg taagcaggcc cgttccaagt acggtgccaa gcgcccgaag gccgcttaat
     7201 tttcgcggcg atacgccgtg gacaaacggt tgtcgccttg aattaaggcg taaccgagta
     7261 agtcgcgatc catgggatcg ctttaaatca aatttatccg ctcgttactt tttttcactg
     7321 ggcggatata gactgaagac acgaaagagg aaatcaaatg ccccgtcgtc gcgaagttcc
     7381 gaagcgcgaa gtgcttgccg acccgaagtt cggcagcgtc gaaattacca agttcatcaa
     7441 cgtgatcatg cttgacggca agaaggccgt ggccgaacgc atcgtttacg gcgccctcca
     7501 gcagatcgaa gaaaagtccg gcaagaacgc cctggaagtg ttcaccaccg ccctgaacaa
     7561 cgtccgtccg ctggtggaag tcaagtcccg ccgcgttggt ggtgccaact atcaggttcc
     7621 ggtcgaagtc cgtcctgtcc gccgtatggc cctcgccatg cgctggctcc gtgaatctgc
     7681 caagaagcgt tctgaaaagt ccatgccgca gcgtctcgcc ggcgaactgc tcgatgcggc
     7741 cgaaggccgt ggcggcgcca tgaagaagcg tgacgaagtc catcgtatgg ctgaagccaa
     7801 caaggccttc tcgcacttcc gcttctaatt cctgttcgct gcatggggaa tttcctcttc
     7861 atgcagcttg tctagtgagg aaccttaaat ggctcgtcaa accccgattt cgcgttaccg
     7921 caatatcggt atctctgccc acattgacgc gggcaagacc accacgtccg aacgtattct
     7981 gttctacacc ggtgtcaacc acaagatcgg tgaagtgcat gatggcgccg ccaccatgga
     8041 ctggatggaa caggaacagg aacgtggcat cacgattacg tccgctgcca ccaccgcctt
     8101 ctggcgcggc atggaaatgc agtgggaacc gtatcgtctg aacatcatcg acaccccggg
     8161 gcacgttgac ttcaccgttg aagttgaacg ttccatgcgc gtcctcgacg gcgcctgcat
     8221 ggtctactgc gccgtgggcg gcgtgcagcc gcagtccgaa accgtttggc gtcaggccaa
     8281 caagtaccgc gttccccgtc ttgccttcgt gaacaagatg gaccgtaccg gtgccaactt
     8341 cttcaaggtc tacgaccaga tgaagaagcg cctcaatgcc aatccggttc ccgtcgttgt
     8401 gcccatcggt gctgaagacc agttcgaagg cgttgtcgac ctcatcaaga tgaagggcat
     8461 catttgggat gcgaagtctc agggcatgac cttcgaatac gtcgacatcc ccgccaacct
     8521 cgttgacgtt gcccaggaat ggcacgaaaa gatggttgag agcgctgcgg aagcttccga
     8581 agagctcatg aacaagtacc tcgaaaacgg cgaactctcc gaagaggaca tcatcgccgg
     8641 tcttcgtcag cgtaccatcg cctgcgaaat ccagccgatg ctttgcggtt ccgccttcaa
     8701 gaacaagggc gtgcagcgca tgctcgacgc cgtcgtgcag ttcttgccgg ccccgtccga
     8761 cattccgccg gttgaaggtt tcgacctcga cgaaaagccg accttccgtg aagccaagga
     8821 cgaagctccg ttcgccgctc tcgcgttcaa gatcatgacc gacccgtacg tcggccagct
     8881 gaccttcctc cgcgtctact ccggccttct gaactccggt gacacggtca tgaactccgt
     8941 caagggtcag aaggaacgta tcggtcgtct gctccagatg cacgctaacg aacgtaagga
     9001 aatcaagttc gtggaagccg gcgacatcgc cgctgccgtg ggcctgaaga acgttacgac
     9061 gggtgacacg ctctgcgcta tcgacgctcc ggtcatcctc gaacgcatgg aattcccgga
     9121 gccggttatt tcccaggctg tcgaaccgaa gaccaaggcc gaccaggaaa agatggctct
     9181 cgctctgaac cgtcttgctc aggaagaccc gtccttccgc gtccgcacgg acgaagaatc
     9241 cggccagacg attatttccg gtatgggcga actccacctc gaaattctcg tcgaccgcat
     9301 gaagcgcgaa ttcaacgtcg aagcctccgt gggcaagccc caggttgctt accgtgaaac
     9361 gatccgctcc gtggttgaaa acgccgaatg caagttcgcc aagcagtccg gcggccgtgg
     9421 tcagtacggt cacgtcgtgc tcaagctcga accgctcgaa cccggcaagg gcttcgaatt
     9481 cgtcgacgcc atcaagggcg gcgtggttcc gcgcgaatac atcccggccg ttgaaaaggg
     9541 tgtcgaagac acgctcaagg ctggcgtcct cgccggctac ccggttgttg acgtgaaggt
     9601 cacgctccac ttcggttcct accacgaagt cgactcgaac gaaaacgcgt tcaagatggc
     9661 tgcctccatg gccttcaagg aaggtatgcg caaggctgat ccggttcttc tcgagccgat
     9721 catggctgtc gaagtcgaaa cgccggaaga aaagatgggc gacgtcatgg gcgacctttc
     9781 ttcccgccgt ggtgtgatcc agggcatgga cgacctcgtt ggcggcggca agagcatcaa
     9841 ggctgaagtg ccgctggctg aaatgttcgg ctacgctacg cagcttcgct ccctcacgca
     9901 gggccgtgcc acgtacacga tggaattcaa gcactacgct gaagctccgc gcaacgtcgc
     9961 cgaagccgtg atcgccgaca aggccaagta agatctacaa tagaactgtt ttttaacccc
    10021 taatctttaa ggaccaaaaa tggccaaggg taagtttgaa cgtaccaagc cccacgttaa
    10081 cgtgggcacg atcggtcacg tggaccatgg taagaccacg ctgacggctg ctatcaccac
//
LOCUS       DBMDTI010000050         9798 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1975_length_9798_cov_2.442405, whole genome shotgun sequence.
ACCESSION   DBMDTI010000050 DBMDTI010000000
VERSION     DBMDTI010000050.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9798)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1975_length_9798_cov_2.442405"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="ACZPB6_03985"
     CDS             <1..689
                     /locus_tag="ACZPB6_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HIR1183664.1"
                     /translation="AGTVVAKGKGSGFLREPEAMIRFLDEIFSADLPIAISVKTRLGW
                     ASEDEFDRLVDVYNRFPMKRLTVHPRLKSDHYKGSVRLETLDRLYPSLRMKVGYNGDV
                     VTPADAVAMTERYPGIDQMMIGRALMADPALLRKIRGGEPATEAELDDFRRDLFESYT
                     AAFGSLKNAMMRMKEYWFYQLALYDESERPAKEIFKSKTPEAFADAVRRLVETCPLRR
                     DARASWYKPL"
     gene            complement(622..1596)
                     /locus_tag="ACZPB6_03990"
     CDS             complement(622..1596)
                     /locus_tag="ACZPB6_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1183665.1"
                     /translation="MSRRPVVPLSFTRHGDASRPAVILLQGLGMASNAWPAELVDALV
                     ETGLQVVLPDNRDAGKSPRVTGVRVRDVEVLGAVARTLARRKVSAAYDLDDMADDVVA
                     LADGLGIGRFHVAGFSMGGMIAQTLALRHPERILSLASLSSATGNPATGLGRLGTIWK
                     IIRPRGPARTKEEAREEMREILLALSGPAFRGDVEALERMLDRIEDTPLDMEATKRQL
                     LAILASGNRSEALGQIRVPTLVIHGTADPLLPFAAGEETAAVIPGARLEPVEGMGHEV
                     PAALGRRYAELVAGNAARAEPKGGSLIKEACTKRRGRRGEAGRSRRAS"
     gene            complement(1696..2637)
                     /locus_tag="ACZPB6_03995"
     CDS             complement(1696..2637)
                     /locus_tag="ACZPB6_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1183666.1"
                     /translation="MMKKLLAAAVCGAFAASALAAMPEKVTIVYVKSPFNLQNMVMKE
                     KGMLEKAFEKDGVKVEWKSINSGAKQAQAMAAGAVDVSAVMNTASLLMANGSGNPVYV
                     ASGVSHPTDTFAIVGKPGASLSVKNLKGKKVAGPRGTVLHQLLIAALVKEGMSADDVE
                     FLSMDIPAAMTAVTSGRVDAALLAASGIIKAEEAGCRVVTTAKGLVDVNLVMTASKQF
                     AEKHPEALETVVRVEREADEWIKANWQEAVAIGAKEHGISLADAEKLAGWSHYYGVLT
                     DADVKGLEADQKFLIENGLMTTPVDVKSIVLPIALGK"
     gene            complement(2654..3400)
                     /locus_tag="ACZPB6_04000"
     CDS             complement(2654..3400)
                     /locus_tag="ACZPB6_04000"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1183667.1"
                     /translation="MVKVEIRNLRKAFSSDSRTTAPIDGLSLEMPAGGITAVIGRSGC
                     GKTTLLRLLAGLEKPDSGEIRMEDAQGRAAAPRTAVVFQEHRLFPWMSVRRNVEVAVR
                     HLPADERRAKVDAVLALTGLAHASESMPAALSGGMSQRVGLARALAAEPELLLLDEAF
                     SSLDALTRRQLYDEFVRIHAARPVTTVLVTHDVTEAVLLSSRICRLENGVIARTYDNP
                     APYPRSLATPGLGALSEEILRGFLEEEPLL"
     gene            complement(3401..4162)
                     /locus_tag="ACZPB6_04005"
     CDS             complement(3401..4162)
                     /locus_tag="ACZPB6_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1183668.1"
                     /translation="MNRSSLPLLGLVIPAALLAAWWLAAHSGGIAPVLLPSPEAVWEA
                     FVRMAADGSLAAHIGMSAMRTMTGFVLAAAFGIGCGVWFAVRPAAAAASHFVIEFLRL
                     TPPLALIPVLILWLGIDEAPKIAIVFLSSFFPIYLSALTAVKGIDPKLAEVAELLRFT
                     SAERFRMLTLPAAMPGILAGLRMGFGYSWRALVGAELIAASSGLGFLITESSEFGKTD
                     VVFVGIFTIVGLGIAADAVISRLVSALSRRISEAA"
     gene            complement(4375..5175)
                     /locus_tag="ACZPB6_04010"
     CDS             complement(4375..5175)
                     /locus_tag="ACZPB6_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR1183669.1"
                     /translation="MLDFIELFILGIGVGSFGALVGIGGGLIMVPLFMYFMMPPSGST
                     FTNVQEVVGTSLFGVLLNALSGTWAYFRQKRIILSVAMPFALATVPGAFLGSYVSEWF
                     SGPAFSITFGASLAFLGYFMYWSSRNKAANRSADEFDPNELPRSKIWLGIGCSFFVGF
                     ISSILGIGGGVVHVPMMVFLLGFPPLVAVATSTFVLMVSAAIGVVGHALLAHIVWAPA
                     VAVGCGAIVGAQLGARLARKSKPRLIVILLSCVMVALGCQLVWRGFSL"
     gene            complement(5386..6273)
                     /locus_tag="ACZPB6_04015"
     CDS             complement(5386..6273)
                     /locus_tag="ACZPB6_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1183670.1"
                     /translation="MIRSVSLRGFMNINDGRHAFAPGLNVIVAPNGAGGHNLLKLLCA
                     VESAGAFTTRWMTKLGYAQSITGRLRENFRPGCVGDLARGECAVELEHEEAQGTLAFG
                     FKSGSEFVSVDAMPSGTAPGRPVFIPMQELASFVPWFPKLFEMWHVGFEGVWRDTCSV
                     LGAEDTKSEPAAPLAALLDRLEKRLGGRVAEHPDDKSLVVIREGAMVPMSELDSGERR
                     IVMLARLVRNGMISPGMILFWNEPDACMDDELRRLVATSAAAFVEAGVQVFACTHSEV
                     LADLMIGRCPPGLAARIRL"
     gene            complement(6300..7478)
                     /locus_tag="ACZPB6_04020"
     CDS             complement(6300..7478)
                     /locus_tag="ACZPB6_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR1183671.1"
                     /translation="MTAMTELFYRDPYVREFTSKVTSCVEGEKGFEVMLEDTAFYPEG
                     GGQPADHGTIDGAAVFDVRRTPAGIVHYCEKAFDVGQDVKGVLDWERRFDNMQNHTGE
                     HVFSGIVNARFGFDNVGFHMDDDVITCDFSGVMTEEQVAEVERACNEAIVANVEVGIS
                     FPDAEALEKLAYRSKKALKGDVRIVDVPGCDRCACCGVHVRSTGEIGLIKVLSSMKHR
                     GGTRVFFVCGIRALRDYEARIRETRAVSALLSAKPLEISSAVERVLAESAAKDARIAA
                     MNRTIFDFKADAIPAQETPLVVLEEGFTPFELRQFCVQLSEAKKAPFIAVMSETEPGV
                     MSYVCAIPDDLLRPVSIALNKRLNGRGGGAKGFVQGSWKADETAVREAVAAVWAEHTA
                     "
     gene            complement(7620..8213)
                     /locus_tag="ACZPB6_04025"
     CDS             complement(7620..8213)
                     /locus_tag="ACZPB6_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1183672.1"
                     /translation="MSSEKALRTRAAIIAAARGIILKEGIGALTMDRAAQAAGMSKGA
                     CMYHFKNKRALHAALIEDYAAHLDGQMKRHEALFEGRPDETLVPGFVEWFKTFEADSR
                     DWADVGVALLSNFVHDDELMKPVHDWYQRLWFRIQALPEVERLPRFVAIMALEGFFYT
                     RKFGVNPPFAELNAQAYGFINEKLVGEGAVRRTKDVA"
     gene            complement(8419..>9798)
                     /locus_tag="ACZPB6_04030"
     CDS             complement(8419..>9798)
                     /locus_tag="ACZPB6_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HIR1183673.1"
                     /translation="PKTSNYDVVVIGAGGAGFSAAITARNAGANVVLLEKMPAVGGNS
                     LISGAEMNAAKNWVQPKLGINDDSPELHAEDTFKGGDGKGDMKVINVMTHQALDAAKW
                     CRDYLGVRFEDDNLFFFGGHSRKRALIPVGHTGTEFIAKFQAKADELGIPVITNMKAE
                     ELIKNKDGRVVGVKATMDGSEYTFNAKGGVVLATGGFGANPEMVKKYNPKIDERFKTT
                     DAPGSTGEALYMAERAGAELVNMGYIQTYPICDPLSGAIELIADARFDGAIMLNQEGK
                     RFVEELQRRDVLSEAILNQTGRYCWVLWNDNIGKISNTVKAHANEYEAFTKQGIMTTC
                     DDLKCIADFTKIPFDQLQKTVKRVSDMAGKGNDKDFNHRAGLMDMQQGKYYVIKAVPS
                     THHTMGGVRINEKAEALTAEGKVIPGLWAAGEVTGVTHGTNRLGGNAYTDIIVFGRIA
                     GEAAAKAAK"
BASE COUNT         1763 a         3083 c         3263 g         1689 t
ORIGIN      
        1 ccgcgggcac agtcgtcgcc aagggcaagg gttcgggctt tctgcgcgag cccgaggcca
       61 tgatccgctt tctcgacgag atcttcagcg cggaccttcc gatcgcgatt tcggtgaaga
      121 ctcgcctcgg ctgggcctcc gaggacgagt tcgaccgtct cgtcgacgtc tacaaccgct
      181 ttccgatgaa acgcctcacg gtgcacccgc gcctcaagag cgaccactac aagggcagcg
      241 tgcgcctcga gacgctcgac cgcctctatc cgagcctcag gatgaaggtg ggctacaacg
      301 gcgacgtcgt cacgcccgcc gacgcggtcg cgatgacgga gcgctatccg ggcatcgacc
      361 agatgatgat cggccgcgcc ctgatggccg accccgcgct gcttcgcaag atccgtggtg
      421 gcgagcccgc gaccgaagcc gagctcgacg acttccggcg cgatctcttc gaaagctaca
      481 cggccgcctt cggcagcctc aagaacgcca tgatgcgcat gaaggaatac tggttctacc
      541 agctcgccct ttatgacgag agcgagcgcc ccgccaagga gatcttcaag tcaaagacgc
      601 ccgaggcctt cgccgacgcc gtcaggaggc tcgtcgagac ctgcccgctt cgccgcgacg
      661 cccgcgcctc ttggtacaag cctctttgat caggctcccc cccttcggtt cggcccgagc
      721 cgcattccct gcgacaagct cagcataacg ccgtccgagc gccgcaggca cttcgtggcc
      781 catgccttcc acgggctcga gcctcgcgcc cggaatcacg gccgccgtct cttcgcccgc
      841 cgcgaacggc agcagcggat ccgccgtgcc gtgaatgaca agcgtcggaa cccgaatctg
      901 cccgagcgcc tccgacctgt ttccgctcgc gaggatcgca agcagctgcc gcttcgtcgc
      961 ctccatgtcg agcggcgtgt cctcgatgcg gtcgagcatc cgctcgagcg cctcgacgtc
     1021 gccgcgaaag gcgggccccg agagcgcaag caggatctcc cgcatctcct ccctcgcctc
     1081 ttccttcgtc cgcgcgggtc ctctcgggcg gatgatcttc cagatcgtgc cgagcctgcc
     1141 gaggcccgtg gcgggattgc ccgtcgcgga cgagagcgac gcgagcgaga gaatcctctc
     1201 gggatgacgc agggcgagcg tctgcgcgat catcccgccc atggaaaagc ccgcgacgtg
     1261 aaagcgcccg atgccgagac cgtcggcgag cgccacgacg tcgtccgcca tgtcgtcaag
     1321 atcgtaggca gccgaaacct tcctgcgcgc gagcgttctc gccacagcgc cgagcacctc
     1381 gacatccctg acgcgaacgc ccgtcacgcg cgggcttttt cctgcgtccc tgttgtcggg
     1441 cagcactacc tggagtcccg tctcgacaag cgcatcgacg agttcggcgg gccaggcgtt
     1501 cgacgccatg ccgagtccct gcagaagaat gacggcggga cgggacgcat caccatgacg
     1561 cgtgaaggag agcggaacga cgggacggcg ggacatcggg aaaatcctcc tgaacaatca
     1621 gaaaaaaggc ccgggacgcc gccgggatgc gggccggacc ttcgtgacgg cgctgaactt
     1681 gaacgctgtc cgggcttact tgcccagagc gatcggaagc acgatgctct tcacatcgac
     1741 gggcgtcgtc atcaggccgt tctcgatgag gaacttctgg tcggcctcaa ggcccttgac
     1801 gtcggcgtcg gtcagcacgc cgtagtagtg ggaccagccc gcgagctttt cggcgtcggc
     1861 gagcgagatg ccgtgttcct tcgcgccgat ggcgacggcc tcctgccagt tggccttgat
     1921 ccactcgtcg gcctcacgct cgacgcgcac gacggtttcg agcgcctcgg gatgcttttc
     1981 ggcgaactgc ttcgaggcgg tcatgacgag gttgacgtcg acgaggccct tggcggtagt
     2041 cacgacgcgg cagcccgcct cctcggcctt gatgatgccg ctcgcggcaa gaagcgccgc
     2101 gtcgacgcgg ccgctcgtga cggcggtcat ggcggcggga atgtccatgg agaggaactc
     2161 gacgtcatcg gccgacatgc cttccttcac cagagcggcg atgaggagct gatggagcac
     2221 ggtgccgcgg ggacccgcga ccttcttgcc cttgaggttc ttcacggaga gactggcgcc
     2281 gggcttgccc acgatggcga aggtgtccgt cgggtgcgag acgcccgagg cgacgtagac
     2341 gggattgccc gagccgttgg ccatgaggag cgaggcggtg ttcatgacag cggagacgtc
     2401 gacggcgccg gccgccatgg cctgggcctg cttggcgccc gagttgatgg acttccactc
     2461 gaccttgacg ccgtccttct caaaggcctt ctcgagcatg cctttttcct tcatgaccat
     2521 gttctgaagg ttgaacgggc tcttcacgta gacgatcgtc accttttcgg gcatggcggc
     2581 gagggcggac gcggcaaagg cgccgcagac ggcggcggca agaagcttct tcatcatttt
     2641 caggatttcc tttttataga agcggctcct cttcaaggaa gccgcggagg atttcttcgg
     2701 aaagcgcgcc gagtccagga gtcgcgaggc tcctcggata aggcgcggga ttgtcgtagg
     2761 tgcgcgcgat cacgccgttc tcaagcctgc agatgcggct cgagagcagc acagcctcgg
     2821 tcacgtcgtg cgtgacgagc acggtcgtga cgggacgcgc ggcgtgaatg cgaacgaatt
     2881 cgtcatagag ctggcggcga gtcagagcgt cgagcgaact gaacgcctcg tcgagcagga
     2941 ggagttcggg ctcggcggcg agcgctcggg caaggcccac gcgctggctc atgccgcccg
     3001 agagcgcggc gggcatcgac tcggaggcat gcgcgaggcc cgtgagcgca agcacggcgt
     3061 cgaccttcgc gcgtcgctcg tcggcgggca ggtgtctcac ggccacctcc acgtttcttc
     3121 tcacgctcat ccagggaaac agcctgtgct cctgaaagac cacggccgtg cggggcgcgg
     3181 cggcgcgacc ctgcgcgtcc tccatgcgga tttcgcccga atcgggcttc tcaagtcccg
     3241 caaggagcct cagcagcgtc gtcttcccgc agccgctgcg gccgatcacg gccgtgatgc
     3301 cgcccgcggg catctcgagc gacagtccgt cgatcggagc cgtcgtgcgg ctgtcggacg
     3361 aaaaggcctt cctgagattt ctgatttcaa ccttcaccat ctacgcagct tccgaaatgc
     3421 ggcgcgagag cgccgaaacg agcctcgaga tcacggcgtc ggccgcaatg ccgagcccca
     3481 cgatcgtgaa gatgccgacg aacacgacgt cggtcttgcc gaattcggag ctctccgtaa
     3541 tgagaaaacc cagccccgat gaggcggcga tgagctcggc acctacgagg gcgcgccagc
     3601 tgtacccgaa gcccatgcga aggcccgcca gaatcccggg catggccgca ggaagcgtga
     3661 gcatcctaaa gcgctcggcg gacgtaaacc tgagaagctc ggccacttcg gcaagcttcg
     3721 ggtcgatgcc cttcaccgcc gtgagcgcgc tcagatagat cggaaagaag ctcgagagaa
     3781 agacgatcgc aatcttgggc gcctcgtcga tgccgagcca gagaatgaga acgggaatga
     3841 gcgcaagcgg aggcgtgagc ctcaggaatt cgatgacgaa atgcgaggcc gccgcggctg
     3901 cgggcctcac cgcgaaccag acgccgcagc cgatcccgaa ggcggccgca agcacgaagc
     3961 ccgtcatcgt gcgcatcgca ctcatgccga tgtgcgccgc cagcgacccg tcggccgcca
     4021 tgcgcacgaa cgcctcccac accgcctcgg gcgacggcag gagaacgggc gcgatcccgc
     4081 ccgagtgcgc ggcgagccac caggcggcaa gaagcgccgc cgggatgaca aggcccagta
     4141 aaggaagcga tgaacggttc atgtcggagc gggcggaccg gagacggcta cggtcatacc
     4201 cgagtaaatt tgtcgggata atgtagcaga tgagcggtcc tgatgcaaac cattctcaca
     4261 cgaggagtcc ggaacgttgg aaaacggcga agaaggcaca gcccctgaag ggacgcgccc
     4321 ggcgctgggc cgggcgtgga aaagacatgc gggacggaag tagtctgtac ctgatcagag
     4381 cgaaaatccg cgccagacaa gctggcagcc gagggcgacc atcacgcagg agagcaggat
     4441 gacgatgagg cggggcttgc tctttcgagc aaggcgtgcg ccgagctgtg cgcccacgat
     4501 ggcgccgcag cccactgcga cggcgggggc ccagacgatg tgggcgagta gcgcatggcc
     4561 gacgacgccg atggcggcgc tcaccatgag cacgaaggta gaggtggcca cggcgacgag
     4621 cggcgggaag ccgagcagga agaccatcat cggaacgtgc acgacgccgc cgccgatgcc
     4681 cagaatggag ctgatgaagc ccacgaagaa ggagcagccg atgccgagcc agatcttgct
     4741 tctggggagc tcgttcggat cgaactcgtc ggccgagcgg ttggcggcct tgttgcggga
     4801 gctccagtac atgaagtagc cgaggaaggc gagcgacgcg ccgaaggtga tcgagaaggc
     4861 ggggcccgag aaccattcgc tcacgtagct cccgaggaag gcgcccggca ccgtggcgag
     4921 tgcaaagggc atcgccacgc taaggatgat gcgcttctgc ctgaagtagg cccaggtgcc
     4981 cgagagtgca ttgaggagca cgccgaagag gctcgtgccc acgacctcct gaacgttcgt
     5041 gaaggtgctg cccgaaggcg gcatcatgaa gtacatgaag agcggcacca tgatgaggcc
     5101 gccaccgatg ccgacgagag cgccgaagga gccgacgccg atgccgagaa tgaagagctc
     5161 gatgaagtca agcatgaccg tgttgtcgtc ttgtattgat gggtgtaacc gagggagtgc
     5221 gcatgacgcg ccgtgtgcgc ggcggaccgt gcgggacttt ccgacatgat acgcccgccg
     5281 tcttccgtca aggacgaaaa cccgccgtcc tcttcatcct gctcaaaaaa tgcggggcgc
     5341 ctaaccatgg gcgccccaca tttcgttttc acagtcttct ttgcgtcaca gcctgatgcg
     5401 cgcggctagc cccggcgggc aacggccgat catcaggtcg gcgagcacct cggagtgcgt
     5461 gcacgcgaag acctgcacgc cggcttcgac gaaggcggcc gcggaggtcg cgacgagcct
     5521 gcgcaactcg tcgtccatgc aggcgtcggg ctcgttccag aagaggatca tgcccggcga
     5581 gatcatgccg ttgcgaacga gccttgcgag catcacgatc ctgcgctcgc cagaatccaa
     5641 ctcggacatc ggcaccatgg cgccttcacg gatgacgacc agactcttgt cgtcaggatg
     5701 ctcggcgacg cggccgccga gcctcttttc gagtcggtca aggagcgccg caagcggggc
     5761 tgcgggttcg gacttcgtgt cctcggcgcc gagcaccgag caggtgtcgc gccagacgcc
     5821 ctcgaacccg acatgccaca tctcgaagag cttcgggaac cagggcacga aggaggcgag
     5881 ctcctgcatg ggaatgaaga cggggcggcc aggggccgtg cccgacggca tggcgtcgac
     5941 ggagacgaat tcggagccgc tcttgaagcc gaaggcgagc gtgccctggg cctcctcgtg
     6001 ctcgagctcg acggcgcact cgccgcgcgc gaggtcgccc acgcagcccg gacgaaagtt
     6061 ctccctcagc cggcccgtga tcgactgcgc gtatccgagc ttcgtcatcc agcgcgtcgt
     6121 gaaggcgccc gcggactcga cggcgcacag cagcttgagc aggttgtgtc cgcctgcgcc
     6181 attgggcgcg acgatgacgt tgagaccggg cgcaaacgca tggcgcccgt cattgatgtt
     6241 cataaagccg cggaggctca cggatcggat caaggccgtt cctccttgca atattagtgt
     6301 taagcggtgt gctcggccca gacggcggcg accgcctccc tgacggcggt ctcgtccgcc
     6361 ttccaactgc cctgcacgaa gcccttcgca ccgccgccgc ggccgttgag gcgcttgttg
     6421 agcgcgatgc tcacggggcg cagaaggtcg tcggggatgg cgcagacgta gctcatgacg
     6481 ccgggctcgg tctcggacat gacggcgatg aagggagcct tcttcgcttc ggagagctgc
     6541 acgcagaact ggcggagctc aaacggggtg aagccttcct ccaggacgac gagcggcgtc
     6601 tcctgagcgg gaatggcgtc ggccttgaag tcgaagatcg tgcggttcat cgccgcaatc
     6661 ctcgcgtcct tggcggccga ctcggcgagc acgcgctcga cggccgagga gatctcgagg
     6721 ggcttcgcgg agagaagcgc ggagacggcg cgggtctcgc ggatgcgggc ctcgtagtcc
     6781 ctgagggcgc ggatgccgca tacgaagaag acgcgggtgc cgccgcggtg cttcatcgag
     6841 gagagcacct tgatgaggcc gatctcgccc gtgctcctca cgtgcacgcc gcagcatgcg
     6901 caacggtcgc atccgggcac gtccacgatg cgcacgtcgc ccttgagggc cttcttgctg
     6961 cggtaggcga gcttttcaag cgcctcggcg tcgggaaacg agatgcccac ctcaacgttg
     7021 gcgacgatcg cctcgttgca ggcgcgctcg acttcggcca cctgctcctc ggtcatcacg
     7081 ccgctgaagt cgcacgtgat gacgtcgtcg tccatgtgaa agcccacgtt gtcaaagccg
     7141 aacctcgcgt tgacgatgcc cgagaagacg tgctcgcccg tgtggttctg catgttgtcg
     7201 aagcggcgct cccagtcaag cacgcccttg acgtcctgcc cgacatcgaa cgccttttca
     7261 caatagtgca cgatgcccgc cggcgtgcgg cgcacgtcga acacggctgc gccgtcgatc
     7321 gtaccgtggt ccgcaggctg tccgccgcct tccggataaa aggccgtgtc ttcaagcatg
     7381 acctcgaagc ccttttcgcc ttccacgcat gaggtcacct tcgaggtgaa ttcgcggaca
     7441 taggggtcac gatagaaaag ttcggtcatg gcggtcatgg acggctcctt gaaaaaaacc
     7501 tgagtggtga gactgcaaag tctatgcgca ttcttcggga tgcgcaaaac gcgcaacgaa
     7561 gacggcccga agccggcgct ccgggccgca ggacaatgca tgcttgggga agacgtgaat
     7621 caggcgacgt ccttcgttct tctgacggcg ccctcgccca cgagcttttc gttgatgaag
     7681 ccgtaggcct gcgcgttgag ttccgcaaag ggcggattga cgccgaactt gcgagtatag
     7741 aagaagccct cgagcgccat gatggccacg aatcgaggca ggcgctccac ttcgggcagc
     7801 gcctggatgc ggaaccagag cctctggtac cagtcgtgca cgggcttcat gagctcgtcg
     7861 tcgtgcacga agttggagag cagcgcgacg cccacgtcgg cccagtcgcg gctgtcggct
     7921 tcgaaggtct tgaaccactc gacgaatccc ggcacgagcg tctcgtcggg acggccctcg
     7981 aagagcgcct cgtggcgctt catctggccg tcgagatgag cggcgtagtc ctcgatgagc
     8041 gcggcgtgga gcgcgcgctt gttcttgaag tggtacatgc acgcgccctt gctcatgcct
     8101 gcggcctggg ccgcgcggtc catggtgagc gcaccgatgc cttccttgag gatgatgccg
     8161 cgggcggcgg cgatgatggc tgcgcgggtt cggagggcct tttcactgct catgggaaat
     8221 gcttgccgga aatgtaggta gatggtggtc actgacccga ttttacgccc tgatgcgttg
     8281 aaacgggcgc ggcgcatggg tttgcaggga ggacgccgca tatggacaac ggcccgcgcc
     8341 ggagactggc gcgggccgct gaccttgatc agggtcccgg agaggaggca tgggaccctg
     8401 aaggagcctt ttcgcgaatt acttcgcagc cttggcggcg gcttcaccgg cgatgcggcc
     8461 gaatacgatg atgtccgtgt aggcattgcc gccgagacgg ttcgtgccgt gcgtgacgcc
     8521 ggtcacttca cccgcagccc agagacccgg gatgaccttg ccttcggccg tcagggcctc
     8581 ggccttttcg ttgatgcgaa cgccgcccat cgtgtggtgg gtggacggaa cggccttgat
     8641 gacgtagtac ttgccctgct gcatgtccat gaggcctgcg cggtggttga agtccttgtc
     8701 attgcccttg ccggccatgt cggagacgcg cttgaccgtc ttctgaagct ggtcgaacgg
     8761 gatcttcgtg aagtcggcga tgcacttcag atcatcgcag gtcgtcatga tgccctgctt
     8821 cgtgaaggct tcgtactcgt tggcgtgagc cttgaccgtg ttggagatct tgccgatgtt
     8881 gtcgttccag agcacccagc agtagcggcc ggtctggttg aggatggctt cggagagcac
     8941 gtcgcggcgc tggagttcct cgacgaagcg cttgccttcc tggttgagca tgatggcgcc
     9001 gtcgaagcgg gcgtcggcga tgagctcaat ggcgcccgag agcggatcgc agatcgggta
     9061 ggtctggatg tagcccatgt tcacgagctc ggcgcccgcg cgttcggcca tgtagagagc
     9121 ttcgcccgtg gagcccgggg cgtcggtcgt cttgaagcgc tcgtcgatct tcgggttgta
     9181 cttcttgacc atttcggggt tcgcgccgaa gccgcccgtg gcgagcacga cgccgccctt
     9241 ggcgttgaac gtgtattcgg agccgtccat cgtggccttg acgccgacga cgcggccgtc
     9301 cttgttcttg atgagctctt cggccttcat gttggtgatg accgggatgc cgagttcatc
     9361 ggccttggcc tggaacttgg cgatgaattc cgtgccggtg tggccgaccg gaatcagggc
     9421 gcgcttgcgg ctgtggccgc cgaagaagaa gaggttgtcg tcctcgaagc gaacgccgag
     9481 gtaatcgcgg caccacttgg cggcgtcaag cgcctggtgc gtcatgacgt tgatgacctt
     9541 catgtcgccc ttgccgtcgc cgcccttgaa ggtgtcttcg gcatggagtt cgggcgagtc
     9601 gtcgttgatg ccgagcttgg gctggaccca gttcttggcg gcgttcattt cagcacccga
     9661 gatgagcgag ttgccgccga cggcgggcat cttttcgagc aggacgacgt tggcgccggc
     9721 gttcctggcg gtgatggcag cggagaagcc cgcaccgccc gcaccgatca cgacgacgtc
     9781 gtagttcgag gtcttcgg
//
LOCUS       DBMDTI010000051         9669 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1996_length_9669_cov_2.126986, whole genome shotgun sequence.
ACCESSION   DBMDTI010000051 DBMDTI010000000
VERSION     DBMDTI010000051.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1996_length_9669_cov_2.126986"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(16..912)
                     /locus_tag="ACZPB6_04035"
     CDS             complement(16..912)
                     /locus_tag="ACZPB6_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1183674.1"
                     /translation="MPVDRSNLTGVALVLLAGILWGAMGTSVQYLFSSGAFETPLELV
                     TLRQLCAGSLFVLVMSLVRPRAIWRLWTDRGMVIDAAVSGALLFGAHNAFFESIYYSN
                     AGTGAILLVTVPLWCGLWSAIVNRSPVPRLEIACFLLAAAGVSLIVTDGDFSGLVFSP
                     MAIVWGLVSSFMAAAYNIQSKRLVARAGVVPALAWGLVFGGVWASVFCNPLSISAEWT
                     AASAGAFAFLVLFGTMFAFWSFLAGLRHVSPVVAGLLNSAEPLAGYFFSMLFLGDVLG
                     FWQTAGIALVIANVALLAFRKN"
     gene            complement(1066..1335)
                     /locus_tag="ACZPB6_04040"
     CDS             complement(1066..1335)
                     /locus_tag="ACZPB6_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR1183675.1"
                     /translation="MNDNEVSDDVLPTNLSGSDVSDGLDRLMRARITPEMHMQCKVCW
                     FVYDPEEGLPEFGIEPGTPFNDLPEDWTCPECGHPKASFIPAEGF"
     gene            complement(1332..2174)
                     /locus_tag="ACZPB6_04045"
     CDS             complement(1332..2174)
                     /locus_tag="ACZPB6_04045"
                     /inference="COORDINATES: protein motif:HMM:NF016682.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation C-terminal
                     domain-containing protein"
                     /protein_id="HIR1183676.1"
                     /translation="MSSLTLDGAGRWAEVPQLPPEVERAAARALVDFLREVEKAVLEA
                     KAAGPEALEELHRVWDFRSFDRAWLPFILPMLGSGEVRITLHDGLARMEETGIPALWR
                     LQMGGHDSFILGRIPRCVWLAARGGEETVGTIVNNGSDVFAAPAILEELRAAQQKIDW
                     SRLPESPAHMVELTKQPLSPGDSRAILSTLGTGDIRAEITGFAKSTINRTGVRGIWHT
                     KMLNNAGKPLLDAYVCAVIPPEVASPCEAFDDTVERCREMVDWVEKDLERGAIGGEPV
                     EEAR"
     gene            complement(2190..2648)
                     /locus_tag="ACZPB6_04050"
     CDS             complement(2190..2648)
                     /locus_tag="ACZPB6_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR1183677.1"
                     /translation="MAKPKPPVEAVVDTAVHPLFARLREEAGFAEFSGATLETMLEAP
                     GLKMVLFAEDPNLRRETLDIAVIGPELRKALGAAVASGWYTAVSEGRALGARYGIRKF
                     PAVALFRGAEYLGAAEGLMGWDEYIQALVEIGAREKGPSRTIAILQKTED"
     gene            complement(2652..2978)
                     /locus_tag="ACZPB6_04055"
     CDS             complement(2652..2978)
                     /locus_tag="ACZPB6_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HIR1183678.1"
                     /translation="MCIAVPMRIVEAEGLSAVARREGAPDLKVDTSLVGELAPGDWVL
                     VFRESVIRTVSEEEAERIGRALACVSAVMAGDESAAAEAAGFEDLTGEPRLPPHLQAL
                     VGKKGA"
     gene            complement(3018..3686)
                     /locus_tag="ACZPB6_04060"
     CDS             complement(3018..3686)
                     /locus_tag="ACZPB6_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HIR1183679.1"
                     /translation="MALDVLDDRCEVLVLGIGNILWADEGFGPRCVEHFHRLYEDRPE
                     VRVEDGGTLGFYLMNLITSARRILVFDCCDFHEEPGTMMVLRDDDVEPWLTTKISPHQ
                     TGFSELLATAALMGRAPESLVVVGVQPERLDDYGGSLSDTLRNLLDEAVELGRAEVAR
                     WGFDLARRPEGAEVAPLMAACVEEAPYEEGRPCEAEACRIGDERFLVRPAGVHVEDPS
                     FERK"
     gene            complement(3810..5204)
                     /locus_tag="ACZPB6_04065"
     CDS             complement(3810..5204)
                     /locus_tag="ACZPB6_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1183680.1"
                     /translation="MTAPASGDKAAKTRGELLTGPVFPMVVKFGVPTMVSMLTPAIYN
                     LTGAYFVSTLGTTAVAALGIVFSLHMILAAVGIMTGQGCASQTSRLLGAGEYEAAGFI
                     AGTGILLSVLGGVAIGVLGLLFDEPLLRVLGATPTIMPDAVRYAEVLLIGSPVVCASF
                     TFNNLLRSEGLAVVGMRALLAGGILNLALTPAFILGLGFGIEGAAWAAVLSQAAGLLI
                     LVNHYRRKAGLLRIIPPTLERLRRLSPTILRNGMPSLLRNALAAVAASILNLTAGGFG
                     DAAVAAMSIVGRVLMTVNAVMVGLGQGYQPVAGFNWGAGNYARVKKALVSTIVAGTAF
                     TTAAAIPGFIFSESIVSVFAGGDPEVMETGTTAMRWAFAGFPFIAVSMIANMTYQVLG
                     RPKAASFLSSLRQGWCFLPLILTLPGWFGLTGLCASQPLAEVLATLVSLRWIRAIVLE
                     ADDLTAKKNASRKA"
     gene            complement(5201..5983)
                     /gene="nadE"
                     /locus_tag="ACZPB6_04070"
     CDS             complement(5201..5983)
                     /gene="nadE"
                     /locus_tag="ACZPB6_04070"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR1183681.1"
                     /translation="MLANPRQTVDDLAEWIRRYFEENGPKCSAVVGLSGGKDSTVTAA
                     LCVKALGAGRVVGVLMPDGVQADIEDAREIARVLGIRIVEVNIHAAVEGLRGALEASP
                     GLLELSGTDGLTRDAAINMPARIRMTTLYAVGQMLPQGGRVANTCNLSEDWVGYSTKY
                     GDAAGDFSPLSHLLVHEVRQIGHALGLPGSLVDKTPSDGLSGSTDEERLGFTYAVLDR
                     YVLTGECPDEAVRARIDRLHALNLHKLRLMPSFEPAEDARLA"
     gene            6198..7409
                     /locus_tag="ACZPB6_04075"
     CDS             6198..7409
                     /locus_tag="ACZPB6_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1183682.1"
                     /translation="MAALLKLLLFTLLMHPACTSLRFTATLDAIHAGASSFEVGLMLA
                     SISFGPMFLAIAAGRWLDRSGPRAPMLASRLSVMAAAGCAILLPASDLGIAPLFGACV
                     LTGLSFMLTNVVIQRLTGDVTTPKNRRFAFTALSLTTASSNLAAPVVAGYVIERLSYT
                     AVYVWSAVAPLILSVLLFVPAFGIVMKARPRAVRAEAGRTKGSAADFLRDGPMRAVLV
                     ASVIISIAWEVGNLLIPIYAAGVGLTPSEIGWVLGSFAAATFIVRFVTPFLLKVVLEW
                     HMIVLSLLLATAAFAVMPLVTTQGAMMGAAFVLGLGLGASLPNMMSLVYLFSPPERIG
                     EAIGLRMMALNLGKSVFPVLAGLLGNWIGAGSTIWCLAAFAAGGCGYAGAAARTVLSR
                     MKALRDAKIDG"
     gene            complement(7549..8811)
                     /gene="dcuC"
                     /locus_tag="ACZPB6_04080"
     CDS             complement(7549..8811)
                     /gene="dcuC"
                     /locus_tag="ACZPB6_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HIR1183683.1"
                     /translation="MNITIGLALLVVCATVYALIKRYETRLVLLTAGLVMTLIAMDPM
                     AAFKQFDKSMTNGSLIIAICSALGFAGVISLTGCDRHLVRLLTKPLGKLGIFLLPACI
                     LVGFVVATAIPSMAGMSAAVGPTLIPLLVRAGFRPAMAAAAVASCVMAGYFNPGVSHN
                     PFIAKLAGMEVMEFVGGYANVTLMVCGCLVVLMTGVCVAYGDFKKGGFEDAAQTEEEH
                     QDEKVNVLCALAPLVPVALLLGFSFYIPSVKISVATAMLIGMMYAMLVTRADPQKTIS
                     KFFDGMGKGYGSILGIIIAAGVFASGLRATGVIDLFVNYLTHANEVAKIGGSVGPYVF
                     GVLTGSGDAAAFAFNEAVTPHAAEFGMKIDGLGYLAMIAAGLGRMSSPLAGGLILLSG
                     IAGVSPIEVVKRTAPAAIITLGILYFLV"
     gene            9182..>9669
                     /locus_tag="ACZPB6_04085"
     CDS             9182..>9669
                     /locus_tag="ACZPB6_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HIR1183684.1"
                     /translation="MSATIVPPLAVAPMEGLTGFVFRQVFSRHFTGADDWFIPFVTPT
                     TLPQFTDRQMRELAPEANAGIHAVPQLLTRRTADFLWSAKALADMGYDEVNLNFGCPA
                     GTVVAKGKGSGFLREPEAMIRFLDEIFSADLPIAISVKTRLGWASEDEFDRLVDVYNR
                     FP"
BASE COUNT         1681 a         3192 c         3193 g         1603 t
ORIGIN      
        1 tgacaatgca ttcgctcaat tcttcctgaa ggccaggagc gccacgttgg cgatgacaag
       61 cgcgatgccc gcggtctgcc agaagccgag cacgtcgccc agaaagagca tcgagaagaa
      121 atagcccgca agcggctcgg cgctgttgag cagccccgcc accacgggcg agacgtgcct
      181 caggcccgcg agaaagctcc agaaggcaaa catcgtgccg aatagaacga gaaaggcgaa
      241 ggcgcctgcg gacgccgcgg tccattcggc cgaaatcgag agcggattgc agaagacgga
      301 cgcccagacg ccgccgaaga ccagacccca cgcaagcgcg ggcacaacgc ccgcgcgggc
      361 gacgaggcgc ttggactgga tgttgtaggc ggcggccatg aaggacgaaa cgagtcccca
      421 gacgatcgcc atcggcgaga agacaagccc ggagaagtcg ccgtcggtca cgatgaggga
      481 gacgcctgcg gcagcgagca ggaagcacgc gatctcaagc cgcggcacgg ggcttcggtt
      541 cacgatcgcg ctccagagtc cgcaccagag cggaacggtc acgagcagga tcgcgcccgt
      601 gcctgcgttc gaatagtaga tggactcgaa gaacgcattg tgcgcgccga agaggagcgc
      661 gccgctcacg gcggcgtcga tcaccatccc gcggtcggtc cagagcctcc agatcgcccg
      721 cgggcgcacg aggctcatga cgagcacgaa gagcgagcct gcgcagagct ggcgcagcgt
      781 tacgagctcg agcggcgtct cgaaggcgcc cgaggaaaag agatactgca cggacgtgcc
      841 catcgcgccc cacagaatgc ctgcgaggag gactagcgcg acgcccgtga ggttggaacg
      901 gtcgactggc ataaaaaaaa tgaaatctgc gtggttttcc acgcctgttt cgttatctta
      961 tcaggcggaa cgggagctcg cgacaacgca gaaaaggcat cgggcgccgc cttgtcagcg
     1021 acgcccgatg agtcttgatg atgcgcctct tccctgcagc gcccgtcaga atccttctgc
     1081 cggaatgaag ctcgccttgg gatggccgca ctcggggcag gtccagtcct cggggagatc
     1141 gttgaaggga gtgcccggct caatgccgaa ttcgggaagc ccctcttcgg gatcgtagac
     1201 gaaccaacac accttgcact gcatgtgcat ctccggcgtg atgcgtgcgc gcatcaggcg
     1261 gtcgagaccg tcgctcacgt ccgagcctga aaggttcgtc gggaggacgt catcgctcac
     1321 ttcattgtcg ttcatcgggc ctcctccacg ggttctccgc cgatggcgcc gcgctcgagg
     1381 tccttttcga cccagtcgac catctcgcgg cagcgctcga cggtgtcgtc gaaggcctcg
     1441 cagggcgagg ccacctcggg cgggatcacc gcgcagacgt aggcgtcaag gagcggcttg
     1501 cccgcattgt tgagcatctt cgtgtgccag atgccgcgca cgcccgtgcg gttgatcgtg
     1561 cttttcgcaa agcccgtgat ctcggcccgg atgtcacccg tgccgagcgt ggagagaatc
     1621 gcgcgggagt cgcccggcga gagcggctgc ttggtgagtt cgaccatgtg ggcggggctc
     1681 tcggggagcc tcgaccagtc gatcttctgc tgtgcggcgc gcagctcctc cagaatcgcg
     1741 ggagccgcga agacgtcaga gccattgttg acgatcgtgc cgaccgtctc ttcgccgccc
     1801 cgggcggcaa gccacacgca gcgcgggatg cgtccgagaa tgaaggagtc gtggccgccc
     1861 atctgcaggc gccagagggc gggaatgccc gtttcctcca tgcgggcgag cccgtcgtgg
     1921 agcgtgatgc gcacctcacc gctgccgagc atcggcagga tgaagggcag ccaggcgcgg
     1981 tcgaagctcc tgaagtccca gacgcggtga agctcctcga gggcttcggg cccggcggcc
     2041 ttcgcctcaa gcacggcctt ctcgacctcg cgcagaaagt caacgagcgc ccgcgcggct
     2101 gcgcgctcta cctcgggcgg gagctgcggc acctcggccc atcggccggc gccgtccaaa
     2161 gtcagactgg acatggggga tctcctaact caatcttccg ttttctgcag gatcgcaatc
     2221 gtgcgcgaag ggcccttctc acgggccccg atctcgacga gcgcctgaat gtattcgtcc
     2281 cagcccatca gtccctcggc ggcgccgagg tactccgcgc ccctgaagag cgcgacggcg
     2341 ggaaacttgc ggatgccgta gcgtgcgccg agggccctgc cctcggagac ggccgtgtac
     2401 cagcccgagg cgacggcggc gccgagcgcc ttcctgagct cggggccgat cacggcgatg
     2461 tcgagcgtct cgcgcctcag gttcggatcc tccgcaaaga ggaccatctt gaggccgggc
     2521 gcctcgagca tcgtctcgag cgtggcgccc gagaattccg caaagcccgc ctcttcgcgc
     2581 aggcgcgcaa agagcggatg aacagccgtg tcgacgacgg cctcgacggg cggcttgggt
     2641 tttgccatgg atcaggctcc tttctttccg acgagcgcct gcagatgcgg cgggagccga
     2701 ggctcgcccg tcaagtcctc aaagccggcg gcctcggccg ccgccgattc gtcacccgcc
     2761 atcacggcgg agacgcacgc gagcgctcga ccgatccttt cggcctcctc ttcgctcacg
     2821 gtgcggatga cgctctcgcg aaagacgagc acccagtcgc ccggcgcgag ttcgcccacg
     2881 agcgacgtgt cgaccttcag gtcgggcgcg ccctcgcgcc ttgcaacggc cgagaggccc
     2941 tcggcctcaa cgatccgcat gggaacggca atgcacatgc catgcctcct ttttatgcgg
     3001 gcctcctgcg ggaggcctca ttttctctcg aaggacggat cctcgacgtg tacgcccgcg
     3061 ggcctcacga ggaagcgctc gtcgccgata cggcaggcct cggcctcgca gggacggcct
     3121 tcctcatagg gcgcctcctc cacgcaggcg gccatgagcg gcgcgacttc ggccccttcg
     3181 ggacggcggg caagatcaaa gccccagcgc gccacttcgg cacgaccgag ctcgaccgcc
     3241 tcgtcaagca ggttcctgag cgtgtcggaa aggcttccgc cgtagtcgtc gagcctttcg
     3301 ggctgaacac ccacgacgac caggctctcg ggggcgcggc ccatcagggc cgcagtggcg
     3361 agcagttcgg agaatcccgt ctgatgcggg gagatcttgg tggtgagcca tggctcgacg
     3421 tcgtcgtcgc gcagcaccat catcgtgccg ggctcctcgt ggaagtcgca gcagtcgaag
     3481 acgaggatgc ggcgcgcgga cgtgatgagg ttcatcagat aaaagcccag cgtgccgccg
     3541 tcctcgacgc gcacctcagg cctgtcctca tagagcctgt gaaagtgttc gacgcagcga
     3601 ggcccgaacc cctcgtcggc ccagaggatg ttgccgatgc cgagcacgag cacttcgcat
     3661 cggtcgtcta gtacgtcaag cgccatgaat gcccccgttg attgtcctga cctttaatat
     3721 agaccagcac gcctcggtcg gcatcagtca ggcgtagcga atccactacg cactatggag
     3781 gagaggccgc gcgacgttcg gcagggtttt caggccttgc gcgaggcgtt cttcttcgcc
     3841 gtgaggtcgt cggcctcgag cacgatggct cgaatccacc tgagactcac gagggttgca
     3901 agcacctcag caagaggctg ggaggcgcac aggcccgtga ggccgaacca tcccgggagc
     3961 gtgaggatga gcggcaggaa gcaccagccc tgcctgaggc tcgagaggaa ggatgcggcc
     4021 ttggggcggc cgaggacctg ataggtcatg ttggcgatca tcgagacggc gatgaaggga
     4081 aagcccgcga acgcccagcg catggccgtc gtgcccgtct ccatcacctc cgggtctccg
     4141 cccgcgaaca ccgagacgat gctctccgag aatatgaagc ccggaatcgc agccgccgtc
     4201 gtgaaggcgg tgcccgcgac gatggtcgag acgagcgcct tcttgacgcg cgcgtagttg
     4261 cccgcgcccc agttgaagcc cgccacgggc tggtagccct ggccgaggcc caccatcacg
     4321 gcgttcaccg tcatgagcac gcggcccacg atgctcatcg ccgccacggc cgcgtcgccg
     4381 aagccgcctg cggtgaggtt gaggatcgag gccgcgacgg ctgcgagcgc gtttctcaga
     4441 aggctcggca tgccgtttcg cagaatcgtg ggcgagagcc tgcgcaagcg ctcgagcgtg
     4501 ggcggaatga tcctgaggag ccccgccttc ctgcggtagt gattgacgag gatgagcaga
     4561 cccgccgcct gggagaggac ggcggcccag gccgcgccct cgatgccgaa gcccagtccg
     4621 aggatgaagg cgggcgtgag cgcaagattg aggatgccgc ccgcgagcag cgcgcgcatg
     4681 ccgacgacgg caaggccctc gcttctgagc aggttgttga aggtgaagct cgcgcacacg
     4741 acgggcgagc cgatgagaag aacctcggcg tagcgcacgg cgtcgggcat gatcgtgggc
     4801 gtcgcgccca ggaccctgag gagcggctcg tcaaagagta ggcccagcac accgatcgcc
     4861 acgccgccca gcacggagag gagaatgccc gtgcccgcga tgaagcccgc ggcctcgtat
     4921 tcgcccgcac cgaggaggcg cgaggtctgg ctcgcgcagc cctgtcccgt catgatgccg
     4981 acggcggcga ggatcatgtg gagcgagaag acgatgccga gcgcggcgac agccgtcgta
     5041 cccagggtcg agacgaagta ggcccccgtc aggttgtaga tggcgggcgt gagcatgctc
     5101 accatggtgg gcacgccgaa cttgacgacc atcgggaaga cgggccccgt gaggagctcg
     5161 cctctggttt tcgcggcctt gtcgccggac gcgggcgccg tcatgcgagg cgggcgtctt
     5221 cggccggctc gaaggacggc atgaggcgca gcttgtggag gttcagggcg tggagcctgt
     5281 cgatgcgggc cctgactgcc tcgtcggggc actcgcccgt gagcacgtag cggtcaagca
     5341 cggcataggt gaagccgagg cgctcctcgt cggtcgagcc cgagaggccg tccgagggcg
     5401 tcttgtcgac gagcgacccg ggcagtccga gcgcatggcc gatctggcga acctcgtgca
     5461 cgagaaggtg cgagagcggg ctgaagtcgc ccgcggcgtc gccgtacttc gtcgagtagc
     5521 cgacccagtc ctcggaaaga ttgcaggtgt tggcgacgcg cccgccctgc gggagcatct
     5581 ggccgacggc atagagcgtc gtcatgcgga tgcgggcggg catgttgatc gcggcgtccc
     5641 gcgtgaggcc gtccgtgccc gacagctcga gcagtcccgg cgaagcctcg agcgcgcccc
     5701 tgaggccctc gaccgccgca tgaatgttga cctcgacgat cctgatgccg agcacgcgcg
     5761 caatctccct cgcatcctcg atgtcggcct gcacgccgtc aggcatgagc acgcccacga
     5821 cgcgcccggc gccgagcgcc ttcacgcaga gcgcggccgt cacggtcgag tccttgccgc
     5881 ccgagagccc caccacggcg ctgcacttcg ggccgttctc ttcaaaatac ctgcggatcc
     5941 attccgcaag gtcgtccacc gtctgccgag ggttcgcgag cattcgtcac ctttcacgtc
     6001 ttttgcggac ggcgcaccgc gcgccgccct gcctcccgtc attttagcgg ctcacccgcc
     6061 tgagggtgac tctcatttgc aagcccgccc cggtcgggaa aggcgatcct cccgagtaga
     6121 atatgagaga cacgcgaaag attgaagcag acgtcaataa cccccgccta aggcggatgt
     6181 tccctcgcgg caattctatg gcagcgctcc tcaaacttct tctcttcacg cttctgatgc
     6241 acccggcgtg cacgtcgctg cgctttaccg cgacgcttga cgccattcat gcaggggcct
     6301 cgtccttcga ggtgggcctc atgctcgcct cgatctcgtt cgggcccatg ttccttgcga
     6361 tcgccgcggg ccgctggctc gaccgttcgg gtccgcgcgc tccgatgctc gcctcacggc
     6421 tctccgtcat ggcggctgcg ggctgcgcca tcctgcttcc cgcatccgac ctcgggatcg
     6481 cgcctctttt cggcgcctgc gtcctcacgg gcctatcctt catgctcacg aacgtcgtga
     6541 tccagcgcct cacgggcgac gtcacgacgc cgaaaaaccg ccgcttcgcc ttcacggcgc
     6601 tctcgctcac gaccgcgagc tcgaaccttg cggcccccgt cgtcgcgggc tacgtgatcg
     6661 agcgactctc ctacacggcc gtctacgtct ggagcgcggt tgcgcccctc attctctccg
     6721 tgctcctctt cgtgcccgcc ttcgggatcg tcatgaaggc aaggccccgc gccgtcaggg
     6781 cggaggcggg acgcacgaag gggtcagccg cggacttcct gcgcgacggc ccgatgcgcg
     6841 ccgtgctcgt cgcctcggtc atcatctcga tcgcctggga ggtcggcaac ctgctcatcc
     6901 cgatctacgc cgcgggcgtg gggcttacgc cctccgaaat cggctgggtg ctcgggagct
     6961 tcgcggcggc cacgttcatc gtgcgcttcg tgacgccgtt cctcctcaag gtcgtgctcg
     7021 agtggcacat gatcgtgctc tcgctcctgc tcgccacggc ggcctttgcc gtcatgccgc
     7081 tcgtcaccac ccagggcgcc atgatgggcg ccgccttcgt gctcgggctc ggcctcgggg
     7141 cgtcgcttcc caacatgatg tccctcgtct acctcttctc gccgcccgag cgcatcggcg
     7201 aggccatcgg ccttcgcatg atggcgctca acctcggcaa gtcggtcttt cccgtcctcg
     7261 ccgggctcct cggcaactgg atcggcgcgg gctccaccat ctggtgcctc gcggccttcg
     7321 ccgcaggcgg atgcgggtac gcgggcgctg cggcccgcac cgtgctctcg aggatgaagg
     7381 cgctcaggga cgcgaaaatc gatggatgag tcctggatga cggggatgga aagagcaagg
     7441 gcctgcaggc tttggaaagc ctgcgggccc ttcacttgtg tttcaccgcg gatccttttt
     7501 cagccccccg gcccgcctcg tggatgaggc gggttccgac gtccggaatt agacgaggaa
     7561 gtacaggatg ccgagcgtga tgatggcggc gggagccgtg cgcttgacga cttcgatcgg
     7621 ggagacgcct gcgatgcccg agagcaggat caggccgccc gcgagcggag aggacatgcg
     7681 gccgaggccg gcggcgatca tggcgaggta gcccagaccg tcgatcttca tgccgaattc
     7741 ggcggcgtga ggcgtgacgg cttcgttgaa ggcgaacgcg gcggcgtcgc cggagcccgt
     7801 caggacgccg aagacgtacg ggccgacgga accgccgatc ttggcgactt cattggcgtg
     7861 cgtcaggtag ttgacgaaga ggtcgatcac gcccgtggcg cgaagaccgg aggcgaacac
     7921 gccggcggca atgatgatgc cgaggatgct gccgtagccc ttgcccatgc cgtcgaagaa
     7981 cttggagatg gtcttctgcg ggtcggcgcg ggtgacgagc atcgcgtaca tcatgccgat
     8041 gagcatggcg gtcgcaacgg agatcttgac ggacggaatg tagaaggaga agcccagaag
     8101 cagagcgacc ggaacgagcg gagcaagcgc gcagagcacg ttgacctttt cgtcctgatg
     8161 ttcttcttcg gtctgggcgg cgtcttcaaa accgcccttc ttgaagtcgc cgtaggcgac
     8221 gcagacgccc gtcatcagga cgacgaggca gccgcagacc atgagggtca cgttggcgta
     8281 accgcccacg aactccatca cctccatgcc ggcgagcttg gcgatgaacg ggttgtggga
     8341 gacgcccgga ttgaagtagc cggccatcac gcaggaggcg acggcagcgg cggccatggc
     8401 cggacggaag cccgcgcgaa cgagaagcgg gatgagcgtc gggccaacgg cggcggacat
     8461 gccggccatc gacggaatgg cggtggcgac gacgaagccc acgaggatac aggccggaag
     8521 caggaagatg ccgagcttgc cgagcggctt cgtgaggaga cggacgaggt gacggtcgca
     8581 gcccgtgagc gaaatgacgc cggcaaagcc gagcgccgag cagattgcga tgatgagcga
     8641 gccgttcgtc atcgacttat cgaactgctt gaaggccgcc atcggatcca tggcgatgag
     8701 ggtcatcacc agaccggcgg tgagaagcac gagtcgcgtt tcatagcgct tgatgagcgc
     8761 atagacggtg gcgcacacga cgagaagcgc cagaccgatg gtaatgttca tgggattgtc
     8821 tcctgccgca gcatgccccg cgggcgctgc gcagcgtggt tgtgtcggat gtccgcagtc
     8881 cgccgcagcg cttccgggag ccttctccga tgccggaatc cgctctgccg gaccgctccg
     8941 cggccgcctg ttgtgtacag gccgcagatt ttaccaaatg tgtcattcaa gaaccgttgg
     9001 ggaaactcct cgttttttca tccgcccccc aatttcgacg caccggtcgt ctttcgcctc
     9061 cccttcctca ttccggtccg ccgcaccgtc ccgaatgcgt gcccgaggcg ccgatcgggt
     9121 agaatcccga atcccatcag ccacgcatcc atttccaatc ttttttccaa agcaaaccga
     9181 catgtccgcc acgatcgttc cgccgcttgc cgtcgccccc atggagggcc tcacgggctt
     9241 cgtcttcagg caggttttca gccgccactt cacgggcgcg gacgactggt tcattccttt
     9301 cgtgacgccc acgaccctgc cgcagttcac ggaccgccag atgcgcgagc tcgcgcccga
     9361 ggccaatgcg ggcattcatg cggttccgca gcttctgacg cgccgcacgg ccgacttcct
     9421 ctggtccgcg aaggcgctcg ccgacatggg ctacgacgag gtgaacctca acttcggctg
     9481 tcccgcgggc acagtcgtcg ccaagggcaa gggttcgggc tttctgcgcg agcccgaggc
     9541 catgatccgc tttctcgacg agatcttcag cgcggacctt ccaatcgcga tttcggtgaa
     9601 gactcgcctc ggctgggcct ccgaggacga gttcgaccgt ctcgtcgacg tctacaaccg
     9661 ctttccgat
//
LOCUS       DBMDTI010000052         9653 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_1999_length_9653_cov_2.840348, whole genome shotgun sequence.
ACCESSION   DBMDTI010000052 DBMDTI010000000
VERSION     DBMDTI010000052.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9653)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9653
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1999_length_9653_cov_2.840348"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="ACZPB6_04090"
     CDS             <1..568
                     /locus_tag="ACZPB6_04090"
                     /inference="COORDINATES: protein motif:HMM:NF019284.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HIR1183685.1"
                     /translation="TVQLKHMYADHQLHGEALDYFENGQLRSSVNFEHGKQSGPFKLF
                     SDDGQLLEEGMLKNGLRHGPFTVYFPQTGKPARQGTYKMNEYDGELTTWRPDGMKVVQ
                     TYKNGVANGWQKNYNSQGALMGEIMMKNGRATGEFRAYDSRGNIVQEGTHEDNVVSTV
                     KGMSSPRPEPNEVSPNDNGGIMIKEVNE"
     gene            1022..3589
                     /gene="bcsA"
                     /locus_tag="ACZPB6_04095"
     CDS             1022..3589
                     /gene="bcsA"
                     /locus_tag="ACZPB6_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016759 - cellulose synthase activity
                     [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HIR1183686.1"
                     /translation="MFPTSIKHALENIGSLYKNWRAAGSGCIAASFNIVCLGLLWLMA
                     PLESEPLASLRRNKAFFYPQVDFNAPKLFDPLRIFIQTVFLLAVLPPRGHGPRLVRTV
                     VDILRAAGALINRAGDAFGAAALKCVRAVVDDSEEKKPGGRRLPSWVPVVLLVPGLVL
                     AALCITQPFNLHGQIVFLALMFVSMIVLMRIRARITLMLLFVISFVVSGRYLWWRCTS
                     TVSTGNGLDLFFGGLLLVAEVYAFSVMVLGYFQVCWVLDRKPAALPADRSVWPHVDIF
                     IPTYNESLDVIKPTVYAALNMDWPADKLHVYVLDDGSRDFIQAFAEAAGAGYIKREEH
                     NHAKAGNINHAMTVTSGEYIVIFDCDHVPSNDFLVSTTGWLVRDPKIALVQTPHHFYS
                     PDPFEKNMHLERAMPIENSLFHDFIQKGNDTWNATMFCGSSAVMRRKALEEIGGIAVE
                     TVTEDAHTSLKLNRRGWNSAFISRPLASGLSTETLAAHIGQRIRWARGMIQIFRLDNP
                     MLGKGLTLPQRLCFFNAMLHFLHGLPRIIFLLAPLPYMYADIYVIYATAASVFAYVIP
                     HMVHTAVTNSILQKGYRYPFLGGVYEAVLSWYILIPTTVALIFPHYGKFNVTAKGGTI
                     GEKYLDWHISWPFVALIALNAVGLFIGLEKAFFDPDPQYVTLVINLGWIAYNLMVLGA
                     AMSVAVEEKEAHRFPRVGLNLPIVLETGDGMRHNVRTVEYSQKELRVRALDTAFTMPA
                     AGERVAFEFAEEAGPVRFEGTVIENGEGWTDIAVDLPDMACERRWNSVTFSRRGMWAM
                     NPEGTVDDRFLTGFLMLGRHALYGYRSMIEFLPGRVLPAVRDAVLSMLPRQPVARKS"
     gene            3613..5835
                     /locus_tag="ACZPB6_04100"
     CDS             3613..5835
                     /locus_tag="ACZPB6_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HIR1183687.1"
                     /translation="MKNPTLIGTAAACLMAAAFAAPAWSAPSDGMKSVSQLQPVWRTD
                     VTGSRTEVVPLMKLVPGGSAAAVKLTGLSRVQAFDFGVRRDEVVSEATLDLSFTPSPA
                     LAPSGSQINFYLNGRLQRSVPISASMVGKPSQLTLKLSPKVIESVNQLTVEFIGHTPS
                     VCENPADAAIWLDIAAESRLTLVKQRVRLANDLAAFPAPFVDTASNEPSVLPMVFTSA
                     PTSMEKQAAAVLASIVGRMSDWRGADFPVFYNGTPAEGPFVVFATNERRPDFLHDLPK
                     ADGPMVTIADAPASLSGKMLVIQGRNDEDLLAAVRALGTDRPVMVGETFRVKKAEVPP
                     VREAYDAPRWINTDAEIPFSKLMQYPGQLSARGHVMAPVQLPVKFAPDLFMVGDAELG
                     MQIRYRYAKPMPEETAQLRTFVNGYLADSDTLAGRDGRGSRTVQLPAFYGAISVDDPM
                     GATLTHSTSLGFSVSYERTIQGGSQDNCRSVSLIGHQMEIEPTSTLTVKGLFHHAELP
                     NLKLFTRAGYPFTKYADLSQTAVFIPQDASRSELTTMLNTMGRMSAMTGAPAMHVHVT
                     GDMNDPELAKRDILAFARFPAPVTDIDVENAGRIQEKLVEAFRAKSPSVQKTAEGAYD
                     NGIAAIVSAESPLAGGRTLVALLSEGASGAARLNAELVNPAGLGSVTGSVAVVNAAGV
                     TGFDVGEHYTVGNLPWYHKVWMTVIDRPGVLVAAALLSALFVGFGIFLFMRYWIRSRS
                     "
     gene            5835..6920
                     /gene="bcsZ"
                     /locus_tag="ACZPB6_04105"
     CDS             5835..6920
                     /gene="bcsZ"
                     /locus_tag="ACZPB6_04105"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HIR1183688.1"
                     /translation="MFKKMIISAALACAFCSASSAMAAWPVWDEFRNDALDNGRVVDK
                     SDDRKVTTSEGQSYAMFFALVTNDQVTFDGLAAWTADNLSGGDLTKTLPAWLWGRGRG
                     DKWGILDTNNATDSDMWIAWCFLEAGRLWKSEEYTKKGMAMLELLKKEVRTVDNLGSV
                     VLPGRVGFEDNGTVKLNPSYYPLFLFKRFALEDAYWNEVYEGSLRMLLRSSPAGIAPD
                     WALFDKGGRLVPPNGDDYEIGSYNAIRTYLWAGMMSPDDPNRAILMKHFSPMVEATRA
                     LNMPPEKIHTVNLTVNDTGPFGFGACLIPMLGNDRTAGLVRSVVDSERLSGESYYSSM
                     LALYGRGWDEGRFAFDADGRLIFPKGR"
     gene            6926..8545
                     /gene="bcsG"
                     /locus_tag="ACZPB6_04110"
     CDS             6926..8545
                     /gene="bcsG"
                     /locus_tag="ACZPB6_04110"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HIR1183689.1"
                     /translation="MQTRIENGAGPRFTGLGVWNVYFIIAFALAAFGYIELNLLGNAL
                     LMAWLLLPVGPKWLRILRGTLGVAAAAVLLYSESWLPGVDSILANKGGIAGFSLLYMA
                     EFALDFINVKMVGWGLLVFLGYFLVRRYVRVTFFTIVYFLGAVTMPWVQSILPERAPV
                     VTADAGGQTNEAAAPAKTGKADAKAVGEWYDAFLAYEHDRRARFPNGLSEKDTPFDIL
                     LLSICSVSNDDLAVSQLDQHPLFKEFNIRFDSFNAATAYSGPALLRLLNGACGQPSHS
                     ELYGERRPECEIMTRLGTLGYSQRLLMDHSGEYDNFLQSMRDKAGVTATLDNAKYPTR
                     YMGFDDEEIADSLAVLRHWQRTQVKSKAGRTATLINFIALHDGNRLPGRGRAEPFKPR
                     AQEMFDNIRTFLRELERSGRKTMVVIVPEHGAAVRGDKIQVPRLRDIPTMRISRVPVM
                     VKFVGLKGMPNEPIHVTGNTSYLALTSLIGKTLETDYFSKDGGTVPLEQLVHDLPQTN
                     PVSENGTVQTLEYQGREYFRQNGGEWKPYGG"
     gene            complement(8952..9248)
                     /locus_tag="ACZPB6_04115"
     CDS             complement(8952..9248)
                     /locus_tag="ACZPB6_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183690.1"
                     /translation="MSKDITVIRDVLCGHAQGLSLKKIQEVTGVPKTSVKRIIDQAHA
                     TELSIEALLHQPDEAIIELMMPSRRACMNYIEPDWERVFLNYERPRNPPGLQVC"
     gene            complement(9321..>9653)
                     /locus_tag="ACZPB6_04120"
     CDS             complement(9321..>9653)
                     /locus_tag="ACZPB6_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183691.1"
                     /translation="IQPDVSCSMSGNRISVRTLDLNRPFGDIRARLDAIAAREHESGT
                     FFLAKWNDRRSSWNGSYPMNSRKTERLSRQSGTLLSQTGTSLFGTTGLERLPRPDSWS
                     QSRKMERA"
BASE COUNT         1753 a         3204 c         2805 g         1891 t
ORIGIN      
        1 cacagtccaa ttaaagcaca tgtacgctga tcatcagtta catggtgaag ctttagatta
       61 ttttgaaaac ggacaacttc ggagttctgt gaacttcgag catggtaaac agagtggtcc
      121 gtttaaactt ttttctgatg acggtcagct tttagaagag gggatgctta aaaacggcct
      181 acgtcatggt ccttttactg tctatttccc gcaaacaggt aaacccgccc gtcaaggaac
      241 ttataagatg aatgagtacg acggtgagtt gactacatgg cgacctgatg gcatgaaagt
      301 cgttcagact tacaaaaatg gtgttgccaa tggctggcag aaaaactaca actcacaagg
      361 ggcgttgatg ggggaaataa tgatgaagaa tggtcgtgcg acgggggaat ttcgtgccta
      421 tgattcgcgt ggcaatattg ttcaagaggg cacccacgaa gacaatgttg taagcactgt
      481 caaaggcatg tcctcaccaa gaccggagcc gaatgaggtt tcgcccaatg acaatggcgg
      541 gatcatgatc aaagaggtga atgagtagct cactagcgga taaccgtaat taaggtaaaa
      601 gcctagaatg ttgagaggaa agcctggagt ctttctcggc tccgtgtgag gcaagctctc
      661 aaataatccg tttctcgact ggcgagtggg acggtttgga gaggcgtgca gtggggccga
      721 ctcgtgaaag aaaatgtaaa tgcttcaatc gtttgcaagg acttgctgat tgcggaagtc
      781 gcaatcagac aaggttgttg gccgaaaatg cctccagaac agagcaaaaa atggacggct
      841 tgagttcgag gtgtacgagt gtctaggagg agggcggccg cacgactgag cggtgtgtca
      901 actccttctc gaaatccctt gtagatctga gtagaatcta aagatttacc aagggcggct
      961 tccgtcccgg gcccggcggg tgagccccgc acgcctccaa tcccttatcc gtcttcaagg
     1021 aatgtttccg acttccataa agcacgcgct cgagaacatc ggcagcctgt acaagaactg
     1081 gcgtgccgcc ggctctggct gcatcgccgc gtcgttcaat atcgtctgcc tcggtcttct
     1141 ctggctcatg gcgccgcttg agtcggagcc actcgcctcg ttgcgccgca acaaggcgtt
     1201 cttctatcct caggtcgact tcaacgctcc gaagcttttc gatcccctgc gcatcttcat
     1261 ccagacggtc tttctcctgg ctgtccttcc gccgcgcggt cacggcccgc gcctcgtgcg
     1321 cacggtcgtc gacatcctgc gcgccgctgg cgccctgatc aaccgcgcgg gcgatgcctt
     1381 cggcgcagcg gccctcaagt gcgtgcgcgc cgtcgtcgac gacagcgagg agaaaaagcc
     1441 gggcggccgc aggcttccca gctgggtgcc cgtcgtcctg cttgtgccgg gactcgtcct
     1501 tgccgcgctc tgcatcaccc agcccttcaa cctgcacggc cagatcgtct tccttgcgct
     1561 catgttcgtc tccatgatcg tgctcatgcg catccgcgca cggatcacgc tcatgctgct
     1621 cttcgtcatt tcgttcgttg tctctggccg ctacctctgg tggcgctgca cgtcgaccgt
     1681 gagcacgggc aacgggctcg acctcttctt cgggggcctg cttctcgtgg ccgaggtcta
     1741 tgcgttctcc gtgatggtgc tcggctactt ccaggtctgc tgggtgctcg accgcaagcc
     1801 tgccgcgctg cccgccgacc gctccgtctg gccccacgtc gacatcttca tcccgaccta
     1861 caacgagtcg ctcgacgtca tcaagccgac ggtctacgcc gcgctcaaca tggactggcc
     1921 cgccgacaag ctccacgtct atgtgctcga cgacggcagc cgcgacttca tccaggcctt
     1981 tgcggaggcc gcaggtgcgg gctacatcaa gcgcgaagag cacaatcatg ccaaggcggg
     2041 caacatcaac cacgcgatga ccgtcacgag cggcgagtac atcgtgatct tcgactgcga
     2101 ccacgtcccc tcgaacgact tcctcgtctc gacgacgggc tggctcgtcc gtgatccgaa
     2161 gattgcactc gtgcagacgc cgcaccactt ttactcgccc gatcccttcg agaagaacat
     2221 gcatctcgag cgcgccatgc cgatcgagaa ctcgctcttc cacgacttca tccagaaggg
     2281 caacgacacg tggaacgcca cgatgttctg cggttcaagc gccgtcatgc gccgcaaggc
     2341 tctcgaggag atcggcggca ttgcggtcga gaccgtgacc gaagacgccc acacgtctct
     2401 caagctcaac cgcaggggct ggaactcggc cttcatctcg cgtccgctcg cttcgggcct
     2461 ctccacggag acccttgcgg cccacatcgg ccagcgcatt cgctgggcgc gcggcatgat
     2521 ccagatcttc cgactcgaca atccgatgct cggcaagggc ctgacgcttc cgcagcgcct
     2581 gtgtttcttc aacgcgatgc tgcatttcct gcacggcctg ccgcgcatca tcttccttct
     2641 ggccccgctt ccctacatgt atgcggacat ctacgtgatc tacgcgacgg cggcctccgt
     2701 cttcgcctac gtgatcccgc acatggtgca cacggccgtg acgaactcga tcctgcagaa
     2761 gggctaccgc tatccgttcc tcggcggcgt gtacgaggcc gtcctctcct ggtacatcct
     2821 catcccgacc acggtcgcgc tcatcttccc gcattacggc aagttcaacg tgacggccaa
     2881 gggcggcacg atcggcgaga agtacctcga ctggcacatc tcctggccct tcgtcgcgct
     2941 catcgcgctc aacgccgtcg gactgttcat cggccttgag aaggccttct tcgatccgga
     3001 tccgcagtac gtgacgcttg tcatcaacct cggctggatc gcctacaacc tgatggtgct
     3061 tggcgccgcc atgtcggtcg ccgtcgagga gaaggaggcg caccgcttcc cgcgcgtcgg
     3121 cctcaacctg ccgatcgtcc ttgagactgg agacggcatg cgccacaacg tacgtacggt
     3181 cgaatactcc cagaaggagc ttcgcgtgcg cgccctcgac acggccttca ccatgcctgc
     3241 ggccggcgag cgcgttgcat tcgaattcgc cgaagaggcg ggccccgtcc gcttcgaggg
     3301 caccgtcatt gaaaacggag agggttggac ggacatcgcc gtcgaccttc ccgacatggc
     3361 ctgcgagcgt cgctggaaca gcgtgacgtt ctcccgccgc ggcatgtggg cgatgaatcc
     3421 cgagggcacg gtcgacgacc gcttcctcac gggcttcctg atgctcggcc gccatgcgct
     3481 ctacggctac cgctccatga tcgaattcct gcccggccgg gttctgcccg ccgttcggga
     3541 cgccgtcctc tccatgctgc cgcgacagcc ggttgcaagg aagtcctgat ggcaaacgca
     3601 aactgagaag acatgaagaa tcccacactc atcggaaccg ctgccgcgtg cctcatggcc
     3661 gctgcgttcg ccgctcccgc ctggagcgcg ccctctgacg gcatgaagtc cgtctctcag
     3721 ctccagcccg tctggcgcac agacgtcaca ggctcgcgta ccgaagtcgt tcccctcatg
     3781 aagctcgtgc ccggcggcag tgccgcggcc gtcaagctta caggcctctc ccgtgtgcag
     3841 gccttcgact tcggcgtgcg ccgtgacgag gttgtctctg aggcgacgct cgacctgagc
     3901 tttacgcctt cgcccgcgct cgccccgtcc ggctcgcaga tcaacttcta cctcaacggc
     3961 cgcctgcaac gctcggtgcc catctcggcc tcgatggtgg gcaaaccctc gcagctcacg
     4021 ctcaagcttt cgcccaaggt gatcgagtcc gtcaatcagt tgacggtcga gttcatcgga
     4081 cacacgccgt cggtctgtga aaatccggcc gacgccgcca tctggctcga catcgcggcc
     4141 gaaagccgcc tcacgctcgt caagcagcgc gtcaggctcg ccaacgatct cgcggccttc
     4201 cccgcgccgt tcgtcgacac cgcctcgaac gagccttccg tgctgccgat ggtcttcacc
     4261 tcggctccga cctcgatgga aaagcaggcc gccgccgtgc tcgcgagcat cgtcggccgc
     4321 atgagcgact ggcgcggtgc cgacttcccc gtcttctaca acggcacgcc cgccgagggc
     4381 cccttcgtcg tcttcgcgac gaacgagcgt cgtcctgact tcctgcacga tctgcccaag
     4441 gccgacggtc cgatggtgac catcgccgac gcgcctgcca gcctctccgg caagatgctc
     4501 gtcattcagg gccgcaacga cgaggacctc ctcgctgccg tgcgtgcgct cggcaccgac
     4561 cgtcccgtga tggtgggcga gaccttccgc gtcaagaagg ccgaagtgcc gcccgtgcgc
     4621 gaggcctatg acgctccgcg ctggatcaac accgacgccg agattccgtt ctcgaagctc
     4681 atgcagtatc cgggtcagct ttccgcccgc ggtcacgtaa tggccccggt gcaactgccc
     4741 gtcaagttcg cacccgacct cttcatggtg ggcgacgccg agctcggcat gcagatccgc
     4801 taccgctacg ccaagccgat gcccgaggag accgcccagc tgcgcacctt cgtcaacggc
     4861 tacctcgccg acagcgacac gctcgcgggc agggacggcc gcggttcgcg caccgttcag
     4921 cttcctgcct tctacggcgc catctccgtc gatgatccga tgggcgccac gctcacccat
     4981 tcgacgagcc tcggcttctc cgtgagctac gagcgcacga ttcagggcgg ctcccaggac
     5041 aactgccgct ccgtctcgct catcggccat cagatggaga tcgagcccac gtccacgctg
     5101 acggtcaagg gcctctttca ccacgccgag cttcccaatc tgaagctctt cacgcgtgcg
     5161 ggctatccgt tcacgaagta cgccgacctc tcgcagaccg cagtcttcat tccgcaggac
     5221 gcgagcagga gcgagctcac gacgatgctc aacaccatgg gccgcatgtc cgccatgacg
     5281 ggcgcccccg cgatgcacgt gcacgtgacg ggcgacatga acgatcccga gcttgccaag
     5341 cgtgacattc tcgcctttgc gcgcttcccc gcccccgtga ccgacatcga cgtcgagaat
     5401 gcgggccgca ttcaggagaa gctcgttgag gccttcaggg cgaagtcccc ctccgtgcag
     5461 aagacggccg aaggtgccta tgacaacggt attgccgcca tcgtctcggc agagtccccg
     5521 ctcgcgggcg gccgcacgct cgtggcgctc ctctccgaag gcgcgtccgg cgccgcccgt
     5581 ctcaacgccg agctcgtcaa tcctgccggc ctcggctccg tgaccggttc cgtcgccgtc
     5641 gtcaacgccg ccggcgtgac gggttttgac gtgggcgagc actacaccgt cggcaacctg
     5701 ccctggtacc acaaggtctg gatgaccgtc atcgaccgtc cgggcgtcct cgttgcggcc
     5761 gctctgctga gcgcgctctt cgtgggcttc ggcatcttcc tcttcatgcg ctactggatc
     5821 aggagccgct cgtaatgttc aagaagatga ttatctccgc agcgcttgcc tgcgccttct
     5881 gcagcgcttc gtccgccatg gccgcctggc ccgtctggga cgagttccgc aatgacgcgc
     5941 tcgacaacgg ccgcgtcgtc gacaagagcg atgaccgcaa ggtgacgacg agtgagggcc
     6001 agtcctatgc gatgttcttc gcgctcgtca ccaacgatca ggtgaccttc gacggcctcg
     6061 ccgcctggac cgccgacaac ctctccgggg gcgacctcac gaagacgctt cccgcctggc
     6121 tctggggcag gggacgcggc gacaagtggg gcattctcga caccaacaac gccaccgact
     6181 ccgacatgtg gatcgcctgg tgcttcctcg aggcaggtcg tctctggaag tccgaggagt
     6241 acacgaagaa gggcatggcg atgctcgagc tcctcaagaa ggaggtccgc accgtcgaca
     6301 atctgggcag cgtcgtgctg ccgggccgcg ttggctttga ggacaacggc acggtcaagc
     6361 tcaatccgag ctactacccg ctcttcctct tcaagcgctt cgcgctcgag gacgcctact
     6421 ggaacgaggt ttacgaggga tcgcttcgca tgttgctgag gtcctccccc gcgggcatcg
     6481 cccccgactg ggcgctcttt gacaagggcg gccgtctagt gccgcccaac ggcgacgact
     6541 acgagatcgg cagctacaac gccatccgca cgtatctgtg ggcgggcatg atgtcgcccg
     6601 acgatcccaa ccgcgcgatc ctcatgaagc acttcagccc gatggtcgag gcgacgaggg
     6661 cgctcaacat gccgcccgag aagatccaca cggtcaatct taccgtcaac gacacgggtc
     6721 ccttcggctt cggcgcctgc ctcatcccga tgctcggaaa cgaccgcacc gcgggcctcg
     6781 tgcgcagcgt ggtcgactcc gaaaggcttt cgggcgagag ctactactcg agcatgctcg
     6841 cgctctacgg ccgaggctgg gatgaaggcc gcttcgcgtt cgacgccgac ggccgcctga
     6901 tctttccgaa gggacgataa gacgcatgca gaccagaatt gaaaacggcg ccggcccccg
     6961 cttcacgggg ctcggcgtct ggaacgtcta cttcatcatt gcctttgcgc ttgcagcctt
     7021 cggctacatc gagctcaacc ttctgggcaa cgcgctcctg atggcctggc tcctgctgcc
     7081 cgtcggcccg aagtggctgc gcatcctgcg cggtacgctc ggcgtcgccg cggctgccgt
     7141 gctcctctat tcggagagct ggctgccggg cgtcgacagc atcctcgcca acaagggcgg
     7201 catcgcgggc ttctcgcttc tctacatggc cgagttcgcg ctcgacttca tcaacgtgaa
     7261 gatggtgggc tggggccttc tcgtcttcct cggctacttc ctcgtgcgcc gctacgtgcg
     7321 cgtgacgttc ttcacgatcg tctacttcct cggcgccgtg acgatgccgt gggtgcagtc
     7381 gatcctgccc gaacgcgcgc ccgtcgtgac ggccgacgca ggcggtcaga cgaatgaggc
     7441 cgccgcgccc gccaagacgg gcaaggccga cgccaaggcc gtgggcgagt ggtacgacgc
     7501 cttcctcgcc tacgagcacg accgacgcgc ccgcttcccg aacggccttt ccgagaagga
     7561 cacgccgttc gacatcctgc tcctgagcat ctgctcggtc tcgaacgacg acctcgccgt
     7621 ctcgcagctt gaccagcatc cgctcttcaa ggagttcaac atccgcttcg actcgttcaa
     7681 cgcggcgacg gcctattcgg gcccggccct gctgcgcctg ctcaacggcg cctgcgggca
     7741 gccgtcccac agcgagctct acggcgagcg ccgtcccgag tgcgagatca tgacgaggct
     7801 cggcacgctc ggctattcgc agcgcctgct gatggaccac tcgggcgagt acgacaactt
     7861 cctgcagtcg atgcgcgaca aggcgggcgt caccgccacg cttgacaacg cgaagtaccc
     7921 gacgcgctac atgggctttg acgacgagga aatcgccgac agcctggccg tgctgcgcca
     7981 ctggcagcgc acgcaggtga agtcgaaggc gggccgcacg gccacgctca tcaacttcat
     8041 cgcgctccac gacggcaacc gcctgcccgg ccgcggccgc gccgagccct tcaagccgcg
     8101 tgctcaggag atgttcgaca acatccgcac cttcctgcgc gagcttgaac gctccggccg
     8161 caagacgatg gtcgtgatcg tgcccgaaca cggcgctgcc gtgaggggcg acaagatcca
     8221 ggtcccgcgc ctgcgcgaca tcccgaccat gcgcatttcg cgcgttccgg tcatggtcaa
     8281 gttcgtgggc ctcaagggca tgcccaacga gcccatccac gtgacgggca acacgagcta
     8341 cctggcgctc acgtcgctca tcggcaagac gctcgagacc gactactttt ccaaggacgg
     8401 cggcacggtt ccgctcgagc agctcgtcca tgatctgccg cagaccaacc ccgtgagcga
     8461 gaacggcacc gttcagacgc tcgaatatca gggccgcgaa tacttccgcc agaacggcgg
     8521 cgagtggaag ccctacggcg gctgatttcc gagaccgcag acatgaatga caaggcccgc
     8581 gaacacctgt gtgatcgcgg gccttacttc gtcatggtat cccgggagcg ggcgcaccgt
     8641 cgtcactcgg ggaaatattc ggctgcaatt gcttcaaact tccaaaatgg aacgtttttt
     8701 ctctcaaatt ggaacggggg ttactggctc ctccctctgg cttcattcca aggaaggttc
     8761 tggcctcaaa actcgggggt tgcttaaatc agcggagccc gtagggcgaa gctgatttaa
     8821 gcaacccccg aagcgccgga ccgtgtgcag gccttcaacc atgactgcag agtttggtcc
     8881 tgtgttgcca ggcagaaggt cgagtagctc attacccggc caaggggttc tgcacgcttc
     8941 cggtactgtt cctagcagac ctgaagcccc ggcgggttgc gtggcctctc gtagttgagg
     9001 aacacccttt cccaatccgg ctcaatgtaa ttcatgcaag cgcgtcggga cggcatcatg
     9061 agttcgatga tggcttcatc cggttggtga aggagtgctt cgatcgagag ctcagtggca
     9121 tgggcctgat cgatgatgcg cttgactgag gtcttgggta cccctgtcac ctcttggatc
     9181 tttttcaggg acagtccttg tgcgtggccg cacagtacat ccctgatgac agttatgtcc
     9241 tttgacatgg ttgtctccta taatcccgcg agcccaatgc ttcgggtatt tctagagtat
     9301 caacctcgtt ccgtcaaatg ctacgcccgt tccattttgc gagactggct ccaggaatct
     9361 ggtcggggca accgttccaa gccagtcgtt ccaaagagag aggtaccggt ttgagagagg
     9421 agcgttccgg attggcgcga gagccgttcc gttttgcgag aattcatggg atatgagccg
     9481 ttccatgagc tgcggcgatc gttccacttt gcgagaaaaa acgttccact ttcgtgctcg
     9541 cgcgcagcaa tggcgtcgag ccgggcacga atgtcgccga acggcctgtt gaggtcaagc
     9601 gtgcggaccg agatcctgtt gccgctcatg ctgcaggaga cgtcgggctg gat
//
LOCUS       DBMDTI010000053         9561 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2019_length_9561_cov_2.640381, whole genome shotgun sequence.
ACCESSION   DBMDTI010000053 DBMDTI010000000
VERSION     DBMDTI010000053.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2019_length_9561_cov_2.640381"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="ACZPB6_04125"
     CDS             <1..548
                     /locus_tag="ACZPB6_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014704.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR1183692.1"
                     /translation="YSNGFINSAAFFKMTEIDWIICALLVLSTVVGIMRGVIREVLSI
                     VGWVAGFMLSMSFAGEIADRIPLDSIGYIPRVIIAAVLILVACLFVVGLFGFILRKML
                     EVAALTFEDRILGAAFGFVRGIIVVAACVFFFGLSQSLSSSRMWQQSVMIGPAETVIE
                     WSMPYMPEWIRALRGTKVTGL"
     gene            689..2191
                     /gene="purF"
                     /locus_tag="ACZPB6_04130"
     CDS             689..2191
                     /gene="purF"
                     /locus_tag="ACZPB6_04130"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR1183693.1"
                     /translation="MCGIIGLMSDTPVNQRVYDALHLLQHRGQDAAGIATLDGLQFHF
                     FKGLGLVRDVFRTRNMRDLTGSIGIGHVRYPTQGNATSPDECQPFYVNAPFGIAFAHN
                     GNLTNADDLRRELYDTDRRHLNTTSDSEVLLNVLASELSRATSEGRRLTKTAVFDAVR
                     ALHRRVRGSYACVAVIAGKGMLAFRDPCGIRPICWGSNKLENGFTEYMVSSESVTMIG
                     LGFKTERDLKPGEAMWIDLEGNVSFSQCADEPSLHPCAFEFVYFARPDSTIDGINVYA
                     ARLRLGEILADHVREAVDLKDIDVVMPIPDSARPCAQQLAQVLGLPYREGFIKNRYMG
                     RTFIMPGQTVRKKSVRQKLNAMPVEFEGKSVLLVDDSIVRGTTMTEIVRMAREAGAVK
                     VFVASSAPRVMFPNVYGIDMPTRSELLCGDGKSAEEVAKILGADAVIYPPVEDLNKVL
                     CDMNPSVESFDCSCFDGRYVTGDITPEYLDALDAKAAAAKTAVKAAGSDD"
     gene            2282..2611
                     /locus_tag="ACZPB6_04135"
     CDS             2282..2611
                     /locus_tag="ACZPB6_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR1183694.1"
                     /translation="MDDRNSEGFSTITPEEARAMMDDGDVVILDVREPSEFATGHVPG
                     AINVPLGEIRMGRTLPECPDLDKTVLIYCRSGRRSDIGGRIMAASGYRDVRNFLGVIQ
                     WPYELVR"
     gene            2767..4170
                     /gene="leuC"
                     /locus_tag="ACZPB6_04140"
     CDS             2767..4170
                     /gene="leuC"
                     /locus_tag="ACZPB6_04140"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR1183695.1"
                     /translation="MARTLYRKILDKHVVRAIDANTVLLYVDLHFANEYTSPQAFAGL
                     VERGVKAPVPDAHLCVVDHIIPSADVTPRVIADAASLRQAETLEANCRRNGIDAFYGA
                     NDPHQGIEHVVVAEQGLVRPGMVVICGDSHTTTHGALGALGFGIGTSEIEHILATQTL
                     VYRLAKTMLIRIDGELPEGSTAKDLALTVLRTISARGALGHVVEYQGSAVDALSIEGR
                     MTLCNLTVEAGARGALIAPDEKTTAWVEAHVPDLAGEELEAARSFWRTLAPDEGAVYD
                     RTVTIDASSVRPMVTWGTSPDQCTAIDEPIPEPESFADPIARAAARRGLAYQGLEPGM
                     TMEDVPVQHVFIGSCTNARLADLRAAAGILRGRHVAPGVRAQVVPGSMEVRCQAEEEG
                     IAQIFRDAGFEWRKSGCSLCLCMNDDILEAGVRCASTTNRNFEGRQGRGARTHLVSPQ
                     MAAAAAVTGRFTDWRRL"
     gene            4185..4829
                     /gene="leuD"
                     /locus_tag="ACZPB6_04145"
     CDS             4185..4829
                     /gene="leuD"
                     /locus_tag="ACZPB6_04145"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR1183696.1"
                     /translation="MEKLTSLTAVAAPLPIDNCDTDQIMPKQFLRGIDKSGLARGVFF
                     NMRCNPDGSPRADCVFNRPGFEKTAIICAGPNFGCGSSREHAVWGLMQMGIRAIIASG
                     FGEIFYSNCFNNGLLACRVSPEDAAAIMAALSDTTPVELTVDLVAQEIRAGDGVWSFE
                     VAPRHRTMLMEGLDMIAATLRDMDEIERFRAVHEAEFPWMTGLAGKAKRRLEGR"
     gene            complement(4933..7263)
                     /gene="pbpC"
                     /locus_tag="ACZPB6_04150"
     CDS             complement(4933..7263)
                     /gene="pbpC"
                     /locus_tag="ACZPB6_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HIR1183697.1"
                     /translation="MRRFARLLLAAAVLAAAAGGGLMIWAKTQPKPGLVPEDTHWSQA
                     VTDRDGRLLHLTLSEDGAYRLPVLQGAVSREALDAVVRYEDRFFHSHPGVNPLSIGRA
                     AWSTLTGTRRIGGSTITMQLARRLQGLDTRSWRGKLEQMLWALRYDMHYTKDEILTAY
                     LTLTPYGGNVEGIEAASLVYFGKHASQLTAAESIALSVVPQNPVKRHPVTGPDFDRAR
                     LAVGRKAINDGVVSERLRPVVEGPLKVRGTRALPFEAPHFTRLVEGTRPDPVLKTTLS
                     LPLQESMEKILSENVARLRPYGIENGAMLVVDSRDMSVLAQVGSIDFFNSAIAGEVDG
                     TRAARSPGSTLKPFVYGLALDQGLIHSRTVLLDSQRSFRGYQPGNADKRFRGPLPAVE
                     ALNDSRNVPAVTLASEIRPDLYDFLREAGVPMKHPREHYGLSIVLGGAEASAEDLARL
                     YGTLGNGGLLRPLVRLPDEEPAAARQMLSPESAWIVRRMLAEGGEALKVEGVSVPLLW
                     KTGTSNGFRDAWTAGYAGRYIIVVWLGNFNGRPDPWLQGALVAQPVFRSAAVRLLTDR
                     EFAMSAQDVKRFDEQPAGVRSEPVCRSTGDLATDAQGRVRCTDTVGAWFIPGVSPIRD
                     TGFLKEILVDEATGLRACEEGDGVRRVFVESWPSEYQQRFLEAGIVKTPIPDWKPECR
                     PEGAVSGTPPAILSPRAGTRYYTGTAGPKQAGIVLRASLAPDAKIVYWFADDRFIGAS
                     DAGGSVVWQPEPGSHRVSAVDDLGRRSHRTVTVRQP"
     gene            complement(7295..>9561)
                     /locus_tag="ACZPB6_04155"
     CDS             complement(7295..>9561)
                     /locus_tag="ACZPB6_04155"
                     /inference="COORDINATES: protein motif:HMM:NF036531.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR1183698.1"
                     /translation="PDTRELKVTVKSDAPGRVFLWAVDTGILSLTGYRTPSPVHALLE
                     DRGLQVDTRQTLEGLMPEGMKLPGEAPFGGGFGSRAISADAMANPFRRTMDRAAVWWA
                     GSLETDEKGRTVTVSLPSEFNGGVRLMAVGASEGRVGGASIDTAVRAPFILTPILPGF
                     AAPGDVFTGVVSLNGEAPWKGSVALGLPEGLSAGVLSAPLVLEAPGEATARFDIAAGA
                     VPGAASVTVTATPEKGSAVTRTTSFSIRPAALRTHDVLWGPLADVKTADVTVKARLLP
                     LENETSLTVSTTPLPLAHGLVRSLPEAFWLSSDAALSAAMPWAQLVGAPAAKLPAGDA
                     DALRRDATARIQKALDTIEGQLGWNGLSAWPWGSPSLMATTWALDFVLTLRENGRDVR
                     PELVRRLADAISNALDGLTPDTLGQARTAAWALAQLTREGTLEAERIEALRNRMDARE
                     LDWRNDPAALYIAYAYRLMRLTDEADELQKAAISIDVSKSEGNDTLTLGGIPALASAV
                     RLAPTPEGTAHLLQTLESRPLASLTDRERAFLASALLSAPSNGAHAGKLDAVTLACTA
                     PAAEKRPAESLAKGDGFVTLSAPGCTAFRLSSAASLKGLYHSTTAAGWPAEPHKTASY
                     EGLEVVKRILNEAGEPVTSVKAGDVVTVEIRARRTSGNAESPVVITDLFPGGFAPAST
                     DESLQGVDVRQTAVSEDRMVGVCMLEHQESTFTYRLRAQTHGKYTVPAVQAADGADPT
                     IKAHDKSLTLTVTP"
BASE COUNT         1585 a         3087 c         3251 g         1638 t
ORIGIN      
        1 gctatagtaa cggcttcatc aattctgcag cctttttcaa gatgactgaa atcgactgga
       61 tcatctgtgc cctgctcgtt ctttcgacgg tggtgggcat catgcgcggt gtcatccgcg
      121 aagtgctctc gatcgtcggc tgggtggccg gcttcatgct gagcatgagc ttcgcgggtg
      181 aaatcgccga ccgcattccg ctcgatagca tcggctacat tccgcgcgtg atcattgcgg
      241 ccgttctgat tcttgtggcc tgcctcttcg tcgtcgggct cttcggcttc attctgcgca
      301 agatgctcga ggtggctgca ctcaccttcg aggaccgcat cctcggcgcg gcattcggct
      361 tcgtgcgcgg catcatcgtt gtggccgcct gcgtgttctt cttcgggctg tcgcagtcgc
      421 tcagctcaag ccgcatgtgg cagcagtccg tcatgatcgg acctgccgag accgtgatcg
      481 agtggtcgat gccctacatg cccgagtgga ttcgggcgct cagaggcacg aaggtgacgg
      541 gattgtgacg aatttcatca aggggacctt gcacatgcat ggtccccttg gtcgttttta
      601 gacgactttt ttctctcaac tctcctcaat cacagcctgc cgccgatccc tcgttggtgc
      661 ggcacgggct aaaggaaaaa caagcatcat gtgcgggatt atcggcctga tgtccgacac
      721 gccggtcaac cagcgtgtct atgacgcgct tcacctcttg caacaccgcg gccaggacgc
      781 cgccggcatt gcgacactcg acggccttca gtttcacttc ttcaaggggc tcggcctcgt
      841 gcgtgacgtc ttccgtacgc gcaacatgcg cgacctgacg ggcagcatcg gcattggcca
      901 cgtccgctac ccgactcagg gcaacgcgac gtcgcctgac gaatgccagc ccttctacgt
      961 gaacgcgccg ttcggcattg cgttcgcgca caacggcaac ctgacgaacg cggacgacct
     1021 gcgccgcgaa ctctacgaca cggaccgccg ccacctcaac acgacgagcg actccgaggt
     1081 tcttctcaac gtgctcgcct ccgagctctc ccgcgccacg agcgagggcc ggcggctgac
     1141 gaagacggcc gtcttcgacg cggttcgcgc gctgcaccgc cgtgttcgcg gctcctacgc
     1201 ctgcgtcgcc gtgatcgcgg gcaagggcat gctcgccttc cgcgatccct gcggcattcg
     1261 tccgatctgc tggggctcca acaagcttga gaacggcttc acggagtaca tggtgagctc
     1321 cgagtccgtg acgatgatcg gcctgggctt caagaccgag cgtgacctta agccgggcga
     1381 ggccatgtgg atcgacctcg agggcaacgt ctcgttctcg cagtgcgccg acgagccctc
     1441 gcttcatccg tgcgccttcg agttcgtcta cttcgcccgt cccgactcca cgatcgacgg
     1501 catcaacgtc tacgccgccc gcctgcgtct gggcgagatc ctcgccgacc acgtgaggga
     1561 ggccgtcgac ctcaaggaca tcgacgtcgt gatgccgatt cccgacagcg cccgcccgtg
     1621 cgcccagcag ctcgcccagg tgctcggact gccgtatcgc gaaggcttca tcaagaaccg
     1681 ctacatgggc cgtaccttca tcatgcccgg ccagacggtc cgcaagaaga gcgtgcgcca
     1741 gaagctcaac gccatgcccg tcgagttcga gggcaagagc gtcctgctcg tcgacgactc
     1801 catcgtgcgc ggcacgacca tgaccgaaat cgtgcgcatg gcccgcgagg ccggcgccgt
     1861 caaggtcttc gtggcctcgt ccgcccctcg cgtcatgttc ccgaacgtct acggcatcga
     1921 catgccgacg cgctccgaac tgctctgcgg cgacggcaag agcgccgagg aggtcgcgaa
     1981 gatcctcggc gccgacgccg tgatctaccc gcccgtcgag gacctcaaca aggtgctctg
     2041 cgacatgaac ccgtcggtcg agtccttcga ctgctcgtgc ttcgacggcc gctacgtcac
     2101 gggcgacatc acacccgaat atcttgacgc actcgacgcg aaggccgcgg ccgccaagac
     2161 cgcggtgaag gcggcgggct ccgacgactg aagcagcccg agcctgcgct aaaatcggca
     2221 cgcatccggc acgcacggag cgtgccgatt ctttatgaaa aatacattca ggaggtccga
     2281 tatggacgat cgcaattccg aaggcttctc gaccatcact cccgaggaag ccagagccat
     2341 gatggacgac ggcgacgtcg tcatcctcga cgtgcgcgag ccctcggagt tcgccacggg
     2401 ccacgtgccc ggcgccatca acgtccctct gggcgaaatc cgcatgggcc gcacgcttcc
     2461 cgaatgcccc gatctcgaca agaccgtcct catctactgc cgctcgggcc gacgcagcga
     2521 catcggcggc aggatcatgg cggcgagcgg ctaccgcgac gtgcgcaact tcctcggcgt
     2581 catccagtgg ccctacgagc tcgtgcgctg aacgccgcct tcgaaatccg agggccggcg
     2641 gattcggatt gcaacatcgg gcaggggaca atttctatat gaaaatctat atttaggtgt
     2701 aagattttct acaaatgagc cgcctctcct caggccctga ggcggatgat gtttggagaa
     2761 gtcccgatgg cccgaactct gtaccggaag attctcgaca agcacgtggt gcgcgcgatc
     2821 gacgcgaaca ccgtgctgct ctatgtcgat ctgcattttg cgaacgaata cacgagcccg
     2881 caggcgtttg cgggcctcgt cgagcgaggc gtgaaggcgc ccgttcccga cgcgcacctc
     2941 tgcgtcgtgg accacatcat tccgagcgcg gacgtcacgc cgcgcgtcat tgccgacgcg
     3001 gcctcgctgc gtcaggccga aacgcttgag gccaactgcc gccgcaacgg catcgacgcc
     3061 ttctacggcg cgaatgaccc gcatcagggc atcgagcacg tcgtggtggc agagcaggga
     3121 ctcgtgcgtc ccggcatggt cgtcatctgc ggcgacagtc acaccacgac ccacggggcg
     3181 ctcggcgccc tcggattcgg gatcggaacc tctgaaatcg agcacattct tgcgacgcag
     3241 acgctcgtct atcgtctggc gaagacgatg ctgatccgca tcgacggcga gctgcccgag
     3301 ggcagcacgg ccaaggatct ggcgctcacg gtgcttcgca cgatcagcgc ccgcggtgcg
     3361 ctcggacatg tggtcgagta tcagggcagt gcggtcgatg cgctttcgat cgagggccgc
     3421 atgacgctct gcaacctcac ggtcgaggcg ggcgcccgag gcgccctgat cgcgcccgac
     3481 gagaagacga ctgcgtgggt cgaggcccat gtgcccgatc ttgcgggaga ggagcttgag
     3541 gccgcccgca gcttctggcg gacgctcgcg cctgacgaag gcgccgtcta cgaccgcacg
     3601 gtgacgatcg acgcttcgag cgtcaggccg atggtcacct ggggcacaag tcccgaccag
     3661 tgcacggcga ttgacgagcc gattcccgaa cccgaatcct tcgcggatcc gattgcgcgc
     3721 gcagccgccc gtcgcgggct cgcctatcag ggacttgagc ccggcatgac gatggaagac
     3781 gttcccgtgc agcacgtctt cattggctcc tgcaccaatg cgcgtctggc ggaccttcgc
     3841 gccgcagccg ggatcctccg ggggcggcat gtggcgccgg gcgttcgcgc acaggtggtg
     3901 ccgggttcca tggaggtgcg ctgccaggcg gaagaggagg gcatcgcgca gatcttccgc
     3961 gacgcgggct tcgagtggcg aaagagcggc tgttcgctct gcctctgcat gaacgacgac
     4021 attctggagg cgggcgtgcg ctgcgcctcc accacgaacc gcaacttcga gggtcgtcag
     4081 gggcgaggcg cccgcacgca cctcgtgagc ccgcagatgg ctgcggccgc ggccgtgacg
     4141 ggcaggttca ccgactggcg ccgtctttga gaaggagcat caacatggaa aagctgacct
     4201 cgctcacggc cgtcgccgca ccgcttccga tcgacaactg cgacaccgac cagatcatgc
     4261 ccaagcagtt cctgcgcggc atcgacaagt cggggctcgc ccgcggcgtc tttttcaaca
     4321 tgcgctgcaa tcccgacggc tcgcccagag ccgactgcgt cttcaacagg cccgggttcg
     4381 aaaagaccgc gatcatctgc gcgggtccga acttcggctg cggctcgagc cgcgagcacg
     4441 ccgtctgggg cctgatgcag atgggcatcc gagccatcat cgcatcgggc ttcggcgaga
     4501 tcttctactc gaactgcttc aacaacggtc tcctcgcctg ccgcgtcagc cccgaggacg
     4561 ccgcggcgat catggccgcg ctctccgaca cgacgcccgt cgagctcacg gtcgacctgg
     4621 tcgcgcagga aatccgtgcg ggcgacggcg tctggtcctt cgaggtcgcg ccgagacacc
     4681 gcaccatgct catggaaggc cttgacatga tcgccgccac gctgcgcgac atggatgaga
     4741 tcgagcgctt ccgagccgtg cacgaggccg agttcccttg gatgacggga ctcgcgggca
     4801 aggcgaagcg aaggcttgag ggccgctgac cagcgagagc tgcatgtaca ggaccgggat
     4861 gtctcccggt ccttttttca aaaaaaacgt ccgcatcgca aggatgcgga cgtttcatga
     4921 gcggattcag gctcagggct gcctgacggt cacggtcctg tgcgacctgc ggccgaggtc
     4981 atcgaccgca ctcacgcggt gcgagccggg ctcaggctgc cagacgacgg agccgcccgc
     5041 gtccgaagca ccgatgaagc ggtcgtcggc gaaccagtag acgatcttgg cgtcaggggc
     5101 gaggctggcc ctgaggacga tgcccgcctg cttcgggccc gccgtgcccg tgtagtagcg
     5161 cgtgccagcg cgcgggctca ggatggcggg cggggtgccc gagacggcgc cctcgggcct
     5221 gcattcgggc ttccagtcgg gaatgggcgt cttgacgatg cccgcctcga ggaagcgctg
     5281 ctggtattcg gagggccagg attcgacgaa gactcgcctg acgccgtcgc cttcctcgca
     5341 ggctctcagt cccgtggcct cgtcgacgag gatttcctta agaaagcccg tgtcgcggat
     5401 gggactcacg ccgggaatga accaggcgcc gaccgtgtcg gtgcagcgca cgcggccctg
     5461 cgcgtcggtc gcaaggtcgc ccgtcgagcg gcaaacgggc tcggacctga cgcctgcggg
     5521 ctgttcgtcg aagcgcttca cgtcctgagc ggacatggcg aactcgcggt ccgtgagaag
     5581 gcgcacggcg gcgctcctga agacgggctg cgcgacgagc gcaccctgca gccaaggatc
     5641 gggcctgccg ttgaagttgc cgagccacac gacgatgatg tagcggcccg catagcctgc
     5701 ggtccaggcg tcgcggaagc cgttcgaggt gcccgtcttc cagaggagag gaacggacac
     5761 gccctcgacc ttgagggcct ctccgccctc ggcgagcatg cggcgcacga tccaggcgga
     5821 ttcaggcgag agcatctgtc tggcggctgc tggctcctcg tcgggaaggc gcacgagcgg
     5881 acgcagaagg ccgccgttgc cgagcgtgcc gtatagcctt gcaaggtcct cggcgctcgc
     5941 ttcggcgccg cccagcacga tcgagaggcc gtagtgctcg cgcggatgtt tcatcggaac
     6001 gccggcctcg cgcaggaagt cgtagaggtc gggccttatc tcggaggcga gcgtcacggc
     6061 gggcacgttg cgcgagtcgt tgagggcctc gacggcggga agcggtccgc ggaagcgctt
     6121 gtcggcgttg ccgggctggt agccgcggaa gcttcgctgc gagtcgagaa ggacggtgcg
     6181 cgagtggatg aggccctggt cgagcgcgag gccgtagacg aagggcttga gcgtggagcc
     6241 ggggcttctc gccgcgcggg tgccgtcgac ttcgcccgca atggcgctgt tgaagaaatc
     6301 gatgctgccc acctgggcga ggacggacat gtcgcgcgag tcgacgacga gcatcgcgcc
     6361 gttttcaatg ccgtaggggc gaagccgagc gacgttttcg gagaggatct tctccatcga
     6421 ctcttgaagc ggcaggctca gcgtggtctt gaggacggga tcgggacgcg tgccctcgac
     6481 gaggcgcgtg aagtgcgggg cctcgaaggg aagcgctcgc gtgcccctca ccttgagggg
     6541 accctcgacg acgggacgca ggcgctcgct cacaaccccg tcgttgatcg ccttcctgcc
     6601 cacggcaagg cgtgcacggt cgaagtccgg acccgtgacg ggatggcgct tgacggggtt
     6661 ctggggcacg acggagagcg cgatcgactc ggcggcggtg agctgcgagg cgtgcttgcc
     6721 gaagtagacg agcgaggcgg cctcgatgcc ctcgacgttg ccgccatagg gcgtcagggt
     6781 cagataggcc gtgaggatct cgtccttggt gtagtgcatg tcgtagcgca gcgcccagag
     6841 catctgctcg agcttgccgc gccagctgcg ggtgtcgagt ccctgaagcc tgcgggcgag
     6901 ctgcatggtg atggtcgagc cgccgatgcg gcgggtgccc gtgagggtgg accaggcggc
     6961 tcggccgatc gagaggggat tgacgccggg atgcgagtgg aagaagcggt cctcgtagcg
     7021 cacgaccgcg tcaagcgcct cgcgcgacac cgctccctgg aggacgggga gcctgtaggc
     7081 gccgtcctcg gagagcgtga ggtgaaggag gcggccgtcg cggtcggtga cggcctggga
     7141 ccagtgggtg tcttcgggca cgagcccggg ctttggctgg gttttcgccc agatcatgag
     7201 tccgccgccg gccgctgccg ccagaacggc tgcggccaga agcagacggg caaaccttct
     7261 catgaggtgc tccgtttggt gttgtccgtg aggctcatgg cgtcacggtg agggtcaggg
     7321 acttgtcatg cgccttgatc gtcggatcgg cgccgtcggc cgcctgaacg gccggcaccg
     7381 tatattttcc atgcgtttgc gctcgaagtc gataggtgaa tgtcgattcc tgatgctcga
     7441 gcatgcagac gccgaccatg cggtcctccg aaaccgcagt ctggcggacg tccaccccct
     7501 gcaggctctc gtccgtggag gcgggtgcaa agccgccggg aaagaggtcc gtgatgacga
     7561 cgggggattc ggcgttgccc gaggtgcggc gcgcgcggat ctcgaccgtg actacgtcgc
     7621 ccgccttcac cgacgtgacg ggctcgcccg cctcgttgag gatgcgcttg acgacttcaa
     7681 gtccttcgta ggacgcggtc ttgtggggct cggcgggcca gcccgcagcg gtcgtgctgt
     7741 ggtagagccc cttgagggag gcggcggacg agaggcggaa ggcggtgcag cccggcgccg
     7801 aaagcgtcac gaagccgtcg cccttcgcaa gcgactcggc ggggcgcttc tccgctgccg
     7861 gcgccgtgca ggcgagcgtc accgcatcaa gctttcctgc gtgtgcgccg ttcgagggcg
     7921 ccgagaggag cgcgctcgca aggaaggcgc gttcgcgatc cgtgagcgac gcaagcggcc
     7981 tcgattcgag cgtctgaagg agatgtgcgg tgccttctgg cgtcggcgcg aggcgcacgg
     8041 ccgatgcaag ggcggggatg ccgccgagtg tcagcgtatc gttcccctcg gacttcgaga
     8101 cgtcgatcga aatggcggcc ttctgcagtt cgtccgcttc atcggtgagg cgcatcaggc
     8161 ggtaggcgta cgcaatgtag agcgccgcag ggtcgtttcg ccagtcaagt tcgcgggcgt
     8221 ccatgcggtt tcggagcgcc tcgatgcgct ccgcttcgag cgtgccctcg cgcgtgagtt
     8281 gggccagagc ccaggcggcc gttcgggcct ggccgagcgt gtcgggcgtc aggccatcga
     8341 gggcgttgct gatggcgtcg gcaagcctgc ggacgagttc gggacgcacg tcacgaccgt
     8401 tttcacgcag cgtgagcacg aagtcgaggg cccatgtcgt ggccatgagg ctcggggagc
     8461 cccagggcca tgccgagagg ccgttccagc cgagctggcc ctcgatcgtg tcgagcgcct
     8521 tctggatgcg ggcggtcgca tccctgcgaa gcgcatcggc gtcgcccgcg ggaagcttcg
     8581 cggcgggggc gccgacgagc tgcgcccagg gcatggcggc cgagagggcc gcgtccgaac
     8641 tcagccagaa ggcttcggga agactgcgaa cgaggccgtg cgcaaggggc agcggcgtcg
     8701 tcgagaccgt gagggaggtt tcgttttcca agggaagcag gcgggctttg acggtcacgt
     8761 cggccgtctt cacgtcggcg agcggtcccc agaggacgtc atgggtcctg agggctgcgg
     8821 gcctgatcga gaaggacgtc gtgcgcgtga cggcggatcc cttttcgggc gtggccgtca
     8881 cggtgacgga tgcggcgccc ggcactgcgc ctgcggcaat gtcaaagcgt gcggtggctt
     8941 cgcccggcgc ctcaaggacg agaggcgcgg agagcacgcc tgcgctcagt ccttcgggca
     9001 ggcctagcgc cacggatccc ttccagggcg cctcgccgtt gagggagacg acgcccgtga
     9061 agacgtcgcc aggtgcggcg aaccccggca ggatcggcgt caggatgaag ggcgcgcgca
     9121 cggccgtgtc gatcgaggcg ccgcccacgc gaccctcgga ggcgcccacg gccatgagac
     9181 gcacgccgcc gttgaattcg gatgggagcg acaccgtgac ggtgcggccc ttttcatccg
     9241 tctcaaggct tcccgcccac cagacggccg cgcgatccat cgtgcgcctg aagggattgg
     9301 ccatcgcgtc ggccgaaatg gccctcgagc cgaagccgcc gccgaagggc gcctcgcccg
     9361 gaagcttcat gccttcgggc atcaggcctt cgagcgtctg gcgcgtgtcg acctgaagtc
     9421 ctcggtcttc gagaagcgca tgaacgggag acggcgtcct gtagcccgtg agcgacagaa
     9481 tgcccgtgtc gaccgcccag aggaagacgc gccccggcgc atctgacttc accgtgacct
     9541 tcagctcgcg cgtgtcgggc a
//
LOCUS       DBMDTI010000054         9487 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2032_length_9487_cov_2.168762, whole genome shotgun sequence.
ACCESSION   DBMDTI010000054 DBMDTI010000000
VERSION     DBMDTI010000054.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2032_length_9487_cov_2.168762"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="ACZPB6_04160"
     CDS             <1..185
                     /locus_tag="ACZPB6_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183699.1"
                     /translation="ATDLLSAGIDLKEKALSAVESAKEDIADAVAEAQVKSQEKKARA
                     EEAEAAKVEDKTAEAA"
     gene            225..2744
                     /locus_tag="ACZPB6_04165"
     CDS             225..2744
                     /locus_tag="ACZPB6_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1183700.1"
                     /translation="MLFHLVHEVGNRQRWRTKETLSHASAALIADQISGIAGVTGVSA
                     NPRTGSVIVTYDTGEARAALAEYLAGLAVNPPIRRAERAAFVSAPRGPVVLEPAEVVR
                     RVVPTRAAEAIEVLSENRLVRHVSKAVRSGASNMPVLSFVVRPILRFLGLAGQGMGGI
                     VSRIAPALASPKAAGGEGALAANNYRADDAELDFGPLARFVFLRPFLPVVANAANAFL
                     GAVPIIREGISELFKGRLNVAVLDASALIVSLLRRDFKTAGLLVLLLGMGEMLENYAR
                     RKSMASLAEQLSVKCDVVWVREDEELRQKPLADVTPEDVIVVRSGSVIPVDGVVLDGE
                     AAVNQTAMTGEPLAVHRTTGGAVFAGTVVESGEIGIRPTGIGDGTRLSQIISFVEESE
                     KVKAGIESKALKFADAIVPFNFALAAIVWLVTRDLTRTASVLLVDYSCALRLATPLAI
                     LSSMKEGTARGVVVKGGRYLEALAEVDTVVFDKTGTLTNATPHLSDVVSLTEDMSEDE
                     LLTLSACLEEHFPHPVSRAIVDAAEEKGLAHHDEQHDAEVKYVVAHGICSKVDGETVL
                     LGSRHFIEDDEGVSCEAARPHVERLASQGKTILYVALSGRLIGVLGIEDPIRDEAEGV
                     IKALHARGKKVVMLTGDDERTAAAVAARLGIDAWRAQVLPSDKADEVLRLKDAGAKVL
                     MIGDGINDSPALSAADVGVTMRDGTDIAQEVADVIMAPSLEYLLVALDLGEATMKRIR
                     SNVGISVGLNSAFLAGGLTGILMPAVSALLHNATTIGVCLNAMRPELGHYDGETRYAD
                     ELRKDVADMFNRLGGVIRGVDSEAAGAPRVAVTALEATGVA"
     gene            2755..3357
                     /locus_tag="ACZPB6_04170"
     CDS             2755..3357
                     /locus_tag="ACZPB6_04170"
                     /inference="COORDINATES: protein motif:HMM:NF040096.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMA2 domain-containing protein"
                     /protein_id="HIR1183701.1"
                     /translation="MADIRDCVRSLLPGRARLRHPMLHGLEAESCREVAGMLETIPGV
                     TGVSINPRVGSLLITWNEAETTAETLLETVEGYAAIFFAAGAEDEAGAAAPADEKETS
                     CTACRAVERAAEAAGSVLGRIESAGVGTFGAAARTLMPETSRRNPKRAARILQNRTML
                     GALALSIGALGVRQTGLHLWAGVAFMALLALHLQQHRRVL"
     gene            3394..3768
                     /locus_tag="ACZPB6_04175"
     CDS             3394..3768
                     /locus_tag="ACZPB6_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183702.1"
                     /translation="MQKDSLPTRILKIILAVLVTVLAVSALAAVTALLLYGLVLAAVA
                     LVLLFILSPEDLRAFVKAAGERIDGWIGRLEGLWRSVKEGLDAWGAREMAARGEGDNE
                     RPPAQTGQGDGLPASGEPSEKA"
     gene            complement(3853..4947)
                     /locus_tag="ACZPB6_04180"
     CDS             complement(3853..4947)
                     /locus_tag="ACZPB6_04180"
                     /inference="COORDINATES: protein motif:HMM:NF025124.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1183703.1"
                     /translation="MAAVIPALEPDLRPAYITDRGPVAGSFIRTGDGDKRLTHYEVDR
                     MCESRMQPVWDTEHVSEASVTDLDAAVLKTMVERNRAMTPSVFGGMAGEEILVKTGVL
                     AEGAGRLVPTLGGLLAAGVYPQQFFPRLNIVLTVRGTGETRSINGPVPYMVETAVNLL
                     AERLRTADGFAYPLRACHEAVVNALQHRDYSPEGRGSQVRIDLFDDRLEILNPGGFFR
                     AAVYNRQIHGLAALRNANLTRILECTPCPDADGRPGTVLEPGTQGLLLIDEALEAAGR
                     PRAVVHDWVSAVSLVFFREERIVHWTDFEYDLTTALSERDSIALTEIVRCSGLSRSTV
                     LAKLRRMIDAGLIERMEPLHSPRQRYRLSS"
     gene            complement(4911..5246)
                     /locus_tag="ACZPB6_04185"
     CDS             complement(4911..5246)
                     /locus_tag="ACZPB6_04185"
                     /inference="COORDINATES: protein motif:HMM:NF016232.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AlbA family DNA-binding domain-containing
                     protein"
                     /protein_id="HIR1183704.1"
                     /translation="MQDPNKLLAKIVRRLRTIGTDTQLVEVKEAAWKLPESIGETLSA
                     FMNHTGGILILGLSERRGFTPVEGFNARRLHNALLSACDTITPPCRPDSRSRAPKSWP
                     PSFLRSNPT"
     gene            5518..6780
                     /locus_tag="ACZPB6_04190"
     CDS             5518..6780
                     /locus_tag="ACZPB6_04190"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HIR1183705.1"
                     /translation="MSQRVERIVVKPGHTHYLACHRLCSAARKLGNQAVYIQRQRIFA
                     KEPWLNRVQLDQTVRSECTESYRNMPSAASAQRQTQIIQEQFVSWNKAKKAYLSDPSK
                     FQAMPRLPGYSKKYRTFVVGRNGYKVVDGNLVITDKNDIGFPRLPIRSCENQPLNAKI
                     NETIVGDVRIVPHGNAFFIELTYQVACSTSILLDGSRACLVDVGLRNIVTLASTISGI
                     HPVVVKGGMLKSINAKYNKDAASLQTHGKQAHRRSKVMKRICRINDNLHKVSRFVVKF
                     FLQHDLGTVVIGHNDGWKQNVNLGKVNNQNFVSIPFNRLIHMIRYKAEEYGIKVIVRE
                     ESYTSRQSALDFDPLPNYGDKNANQVLSTGRRVRGLFFRSNGQVINSDVNGCLNIGRK
                     EFGDDWLKKLVETDKGCLMQPVTVRIGQ"
     gene            complement(6975..8435)
                     /locus_tag="ACZPB6_04195"
     CDS             complement(6975..8435)
                     /locus_tag="ACZPB6_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR1183706.1"
                     /translation="MQYIDAKYDLLINGEWVPAEGGETFETFNPANGEKLADCANASP
                     ADIDRAVAAARAAFPAWAAKSPQERAALLLKVADLIDENAARLAMVETLDNGKPIRET
                     TLVDVPLSSDHFRYFAGCLRAEEGSAVMIDENTMSIVLHEPIGVVGQIIPWNFPLLMG
                     AWKIAPALAAGNTLVIKSSSTTPLSLFELGKLLNQVLPAGVVNIVSGRGARTGQAMLD
                     HPGFDKLAFTGSTEVGYSVAEAAAKRLIPSTLELGGKSANIIFDDAQLDKAIEGAQIG
                     ILFNQGQVCCAGSRIFVQEGIYDKFVGELAEAFKKIKVGDPTDMSTQMGAQINERQLS
                     KILGCVEEGVSEGARVLVGGARASEKGAFMQPTLLVDVTNDMKVAREEIFGPVAVVIK
                     FKTEEEVVRMANDSDYGLGGAVWTRDINRAMRVARDVRTGRMWINTYNELPAHTPFGG
                     YKKSGIGRETHKMMLAHYSQVKNIYLSLNEQKRGFY"
     gene            8926..>9487
                     /locus_tag="ACZPB6_04200"
     CDS             8926..>9487
                     /locus_tag="ACZPB6_04200"
                     /inference="COORDINATES: protein motif:HMM:NF023742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1183707.1"
                     /translation="MKANIAYKFELMPTREQKRIFAQAAGWRRFVWNKAIELQKRCLE
                     QEVPFLKYTELAGRLVLWKKEDDMLWLQEAPSQALQQCLQQCLMDASAAIEAFKAKKK
                     GFPKFQARGDGDSFRIPHSASSPDGLGRSQRSSPYPEGRLRAIQKVASDSRQDQADDG
                     FRRMRALVCLGRDGDGFRQRRSASSRR"
BASE COUNT         1751 a         2848 c         3099 g         1789 t
ORIGIN      
        1 tggccacgga cctcctctcc gccggcatcg acctcaagga aaaggccctg tccgcagttg
       61 aaagcgcgaa ggaggacatc gccgacgccg tcgccgaagc ccaggtcaag tctcaggaga
      121 agaaggcccg cgccgaagag gccgaagccg ccaaggtcga ggacaagacg gctgaagccg
      181 cctgatcgcc ggccccgccg gcccgactct ataggcatgc gcacatgctt ttccatcttg
      241 tacatgaggt cggcaaccgc cagcgctggc gtacgaagga gactctttcg cacgcctccg
      301 cggcgctgat tgccgatcag atctcaggca ttgccggcgt caccggcgtg tcggccaatc
      361 cgcgcacggg ctccgtgatc gtgacctacg acacgggcga ggcccgtgca gcgctggccg
      421 aatatctcgc cggccttgcc gtcaatccgc cgatccgccg cgccgagcgc gcggcgttcg
      481 tttccgcgcc ccgcggcccc gtcgtgctcg agcccgctga ggtcgtgcgc cgcgtggtgc
      541 ccacccgcgc cgccgaggcg atcgaggtgc tgagcgaaaa ccgtctcgtg agacacgtct
      601 cgaaggcggt gcgctcgggc gcctccaaca tgcccgtcct ctcgttcgtg gtgcgcccga
      661 tcctgcgctt cctcggactg gcggggcagg gcatgggcgg aatcgtctcc cgcatcgcgc
      721 ccgcgcttgc ctcgccgaag gctgcgggcg gcgagggcgc ccttgccgcc aacaactaca
      781 gggcggacga cgccgagctc gacttcggtc cgcttgcgcg cttcgtcttc cttcgccctt
      841 tcctgcccgt cgttgccaat gcggccaacg ccttcctcgg cgccgtgccg atcatccgcg
      901 agggcatctc cgagcttttc aagggccgtc tcaatgtcgc cgtactcgac gcctccgcgc
      961 tcatcgtctc gctcctgcgc cgcgacttca agaccgccgg cctgctcgtt ctgctgctcg
     1021 gcatgggcga gatgctcgag aactacgcgc gcaggaagtc gatggcctcg ctcgccgagc
     1081 agctttcggt gaagtgcgac gtcgtctggg tccgcgagga cgaagaactg cgccagaagc
     1141 ccctcgccga cgtgacgccc gaggacgtga tcgtcgtgcg ctctggcagc gtcattcccg
     1201 tcgacggcgt ggtgcttgac ggcgaggccg ccgtgaacca gaccgcgatg acgggcgagc
     1261 cgctcgcagt tcaccgcacc acgggcggcg ccgtctttgc cggcaccgtc gtcgagagcg
     1321 gcgagatcgg cattcgtccg acgggcatcg gcgacggcac gcgcctctcg cagatcatca
     1381 gcttcgtcga ggaaagcgag aaggtgaagg cgggcatcga gagcaaggcg ctcaagttcg
     1441 ccgatgcgat cgtgcccttc aacttcgcgc tcgcggccat cgtctggctc gtcacgcgcg
     1501 atctgacccg cacggcctcc gtgctgctcg tcgactacag ctgcgcgctg cgcctggcga
     1561 cgccgcttgc catcctctcc tccatgaagg aaggcacggc ccgaggggtc gtcgtcaagg
     1621 gcggccgcta cctcgaggcg cttgccgaag tcgacaccgt cgtcttcgac aagacgggca
     1681 cgctcaccaa cgccactccg cacctctccg acgtggtctc cctcacggag gacatgtccg
     1741 aggacgagct gctgacgctc tccgcctgtc ttgaggagca cttcccgcat cccgtctcgc
     1801 gcgccatcgt ggacgcggcc gaagagaagg ggcttgcaca ccatgacgag cagcacgacg
     1861 ccgaggtgaa gtacgtcgtc gcccacggca tctgctcgaa ggtcgacggc gagaccgtgc
     1921 tgctgggttc gcgccacttc atcgaggacg acgaaggcgt gagctgcgaa gccgcacgcc
     1981 cccacgtcga gcgtctggcc tctcagggca agaccattct ctacgtcgcg ctttcgggcc
     2041 gcctcatcgg cgtgctcggc atcgaggatc cgatccgcga tgaggctgaa ggcgtcatca
     2101 aggcactgca cgcccgcggc aagaaggtcg tcatgctcac gggtgacgat gagcgcacgg
     2161 ctgcggccgt cgcggcacgc ctcggcatcg acgcctggcg cgctcaggtg cttccgtccg
     2221 acaaggccga cgaagtcctg cgtctcaagg acgcgggcgc gaaggtcctg atgatcggcg
     2281 acggcatcaa cgacagtccc gcgctgagcg cggccgatgt cggcgtgacg atgcgcgacg
     2341 gcacggacat cgcgcaggag gtggcggacg tcatcatggc gccttcgctc gaatacctgc
     2401 tcgtcgccct tgacctcggc gaagccacga tgaagcgcat ccgcagcaac gtcggcattt
     2461 ccgtgggcct caactcggcg ttccttgcgg gcggcctcac gggcattctg atgccggccg
     2521 tctcggcgct gcttcacaac gccacgacga tcggcgtgtg cctcaacgcc atgcgccccg
     2581 aactcggcca ctacgacggg gagacccgtt atgcggacga actccggaag gacgtggccg
     2641 acatgttcaa ccgcctgggc ggcgtgatcc gcggcgtcga ttcggaggcc gccggcgctc
     2701 cgcgcgttgc cgtgaccgcg ctcgaagcca cgggcgtcgc ataaggagac agacatggca
     2761 gacattcgcg actgcgtgag aagcctgctg ccgggacgcg cgcgcctgcg ccatcccatg
     2821 cttcacggcc tggaggccga gtcttgcagg gaggtcgcgg gcatgctcga gaccattccc
     2881 ggcgtgacgg gcgtgtcgat caacccgcgc gtgggctcgc tcctcatcac ctggaacgag
     2941 gccgagacga cggccgagac gctgcttgag acggtcgagg gctacgccgc catcttcttc
     3001 gccgcaggcg cagaagatga ggctggggcc gctgcgcccg cggacgaaaa ggagacgtcc
     3061 tgcacggcat gtcgtgccgt cgaaagggcg gcggaagccg ccgggagcgt gctcggcagg
     3121 atcgagtccg caggcgtcgg caccttcggc gcggccgccc gcacgctcat gcccgaaacg
     3181 tcgaggcgca atccgaagcg cgccgcccgc atcctgcaga accgcacgat gctcggcgcc
     3241 cttgcgctct cgatcggcgc gctcggcgtc aggcagacgg ggctgcatct ctgggcgggc
     3301 gtcgccttca tggcgcttct tgccctgcac ctgcagcagc accgccgcgt cctctaaaat
     3361 acgggcattc accaccgccg ccacggattg cgcatgcaga aagactccct cccgaccagg
     3421 atcctgaaga ttatcctcgc cgtgctcgtc acggtactgg ccgtctccgc cctggccgcc
     3481 gtgacggcgc tccttctcta cggcctcgtg ctggcggcgg tggcgctcgt gctccttttc
     3541 attctttcgc ccgaggacct cagggccttc gtcaaggcgg ccggagaacg catcgacggc
     3601 tggatcggcc gcctcgaggg gctctggcgc agcgtcaagg aggggctcga cgcctggggc
     3661 gcccgcgaaa tggccgcgcg cggcgagggc gataatgagc gcccgcccgc tcagacaggg
     3721 cagggcgacg ggcttcccgc tagcggcgag ccttcggaaa aggcatagtg ccggacggcg
     3781 aaagagacat cgaaaagagg cggactccgc agggagccgc ctcttttttg taagccgttc
     3841 ggcagtccgt cttcaactgg agagcctgta gcgctgccgc gggctgtgga gcggttccat
     3901 gcgctcgatg aggcctgcgt cgatcatgcg gcggagcttg gcgagcacgg tgcttctcga
     3961 gaggcccgaa catcgcacga tctcggtgag cgcaatcgag tcacgctcgg agagcgccgt
     4021 cgtgaggtcg tattcgaagt ccgtccagtg cacgatgcgc tcctcgcgga agaagacgag
     4081 cgaaacggcg gagacccagt cgtgcacgac ggctctgggt ctgcctgcgg cctcaagcgc
     4141 ctcgtcgatg agaagcagcc cctgagtgcc aggctcgagc acggtgccgg gccttccgtc
     4201 cgcatcaggg cagggcgtgc actcgagaat gcgtgtgagg ttggcgttgc gaagcgccgc
     4261 cagaccgtgg atctggcggt tgtagacggc cgccctgaag aaaccgcccg gattgaggat
     4321 ctcgagcctg tcgtcgaaga ggtcgatgcg cacctgcgag ccacggcctt cgggcgaata
     4381 gtcgcggtgc tgaagcgcgt tcacgaccgc ctcgtggcag gcgcgaagcg gatatgcgaa
     4441 gccgtccgcc gtcctcaggc gctctgcaag gaggttcacg gccgtctcga ccatgtaggg
     4501 cacggggccg ttgatggaac gggtttcacc cgttcctctg accgtgagga cgatgttgag
     4561 ccttggaaag aactgctgcg gatagacgcc cgccgcgaga agtccgccga gcgtcgggac
     4621 aaggcgtccc gcgccctcgg cgagcacgcc cgtcttgacg agaatctcct cgcccgccat
     4681 gccgccgaag acggagggcg tcatggcgcg gttgcgctcg accatcgtct tgaggacggc
     4741 ggcgtcgagg tccgtgacgg acgcctcgga gacgtgctcg gtgtcccaga cgggctgcat
     4801 gcggctttcg cacatgcggt cgacctcgta gtgcgtcagt cgcttgtcgc cgtcgcccgt
     4861 gcggatgaag cttccggcca cggggccgcg gtccgtgatg taggcgggcc tcaggtcggg
     4921 ttcgagcgca ggaatgacgg cggccacgac ttcggcgccc tcgaacggct gtcgggtctg
     4981 cagggaggcg tgatggtgtc gcaggcggaa aggagcgcgt tgtgaaggcg tcttgcgttg
     5041 aagccctcga ccggtgtgaa gccgcggcgc tcggagaggc cgaggatgag gatgccgccc
     5101 gtgtggttca tgaaggccga aagcgtctca ccgatgctct cgggcagctt ccaggcggcc
     5161 tccttcactt cgacgagctg agtgtccgtg ccgatggtgc gcaggcggcg cacgattttg
     5221 gcgagcagct tgttcggatc ctgcatgatg cacgctcctt cttcgcgggc ttttgcagga
     5281 agtgtacgaa gtcgtgcggc aagtgcacca gaggattaac aaaacccgcg taattcctca
     5341 tccaatgcga cgcattgggt ggggatgtat agcgggccga gcgttagctc ggctacagct
     5401 gtatagtatt gagtacggtt ctggtgctca tttttccatg attcgacacc accaaatccc
     5461 aatggatgta actgttgcag acgctgtctg cgcggtttcg aaataacggt ctttgaaatg
     5521 tctcaacgag tggaacggat agtcgtcaag cctgggcata cgcattacct cgcgtgccat
     5581 aggctgtgtt ctgccgcccg caagttgggc aatcaggccg tctacatcca acggcagaga
     5641 atttttgcca aagaaccttg gttgaaccga gttcagttgg atcaaaccgt tcggagcgaa
     5701 tgcaccgagt cgtatagaaa tatgccttcg gctgcatccg ctcaacgaca gactcagatc
     5761 atccaagagc aattcgtttc atggaataag gccaagaaag cttatctgtc tgatccgagc
     5821 aagttccagg cgatgccgcg actgccgggt tacagcaaaa agtatcgaac tttcgtcgtt
     5881 gggcgtaatg gctataaggt tgtcgatggc aatcttgtta tcaccgacaa gaacgacatt
     5941 ggttttcctc ggttgccaat ccgctcttgt gaaaatcagc ctctcaacgc caaaataaat
     6001 gaaacgatag ttggcgacgt gcgaattgtt ccgcacggga acgccttttt catcgaactg
     6061 acctatcagg tcgcttgttc gacctccatc ctgctcgacg gcagccgtgc gtgcttggtt
     6121 gatgttgggc ttcgcaatat cgtcactctt gcttcgacga tcagcgggat tcatcccgtg
     6181 gttgtcaaag gtggcatgtt gaaaagcatc aacgccaagt acaacaagga cgccgcaagt
     6241 cttcagactc atggaaagca agcgcatcgt cgctccaagg tcatgaagcg catttgccgc
     6301 atcaatgaca acctgcacaa ggtttcccgc ttcgttgtga agtttttcct tcaacatgac
     6361 ttgggaaccg ttgtcatcgg gcataacgac gggtggaagc agaacgtcaa ccttggcaag
     6421 gtcaacaatc aaaattttgt gagcattccg ttcaatcgct tgatccatat gattcgctac
     6481 aaggcggagg agtacggcat caaggtgatt gttcgtgagg aaagctacac ctcgcgtcag
     6541 tctgcgcttg acttcgatcc attgccgaat tacggcgata agaatgcgaa tcaggttctt
     6601 agcacgggca ggcgagtgcg aggactgttt ttccgatcga acggtcaagt tatcaactct
     6661 gatgtcaacg gatgcttgaa cattggcaga aaagaattcg gtgatgactg gctcaaaaag
     6721 cttgtcgaaa ccgataaggg ctgtttgatg cagcccgtca cagtccgtat tggacagtga
     6781 ctagaatggg ggccacggcc cccagaatcc ccttccaatc cctaatgaca tgggattggg
     6841 aggggagcat caacccagtg gatgccagag gctgagaatg aggacgggca tgaagaaggg
     6901 gccggcgctt tcgcgctggc cccttcaggc ggagacggga tctgggagcg gaggtccgga
     6961 cctcctgcgc cctgttagta gaagccgcgc ttctgttcgt tgagcgagag gtagatgttc
     7021 ttcacctggc tgtagtgggc gagcatcatc ttgtgggtct cgcggccgat gccggacttc
     7081 ttgtagccgc cgaacggggt gtgcgcgggc agctcgttgt aggtgttgat ccacatgcgg
     7141 cccgtgcgga cgtcgcgggc gacgcgcatg gcgcggttga tgtcgcgggt ccagacggcg
     7201 ccgccgaggc cgtagtcgct gtcgttggcc atgcggacga cttcctcttc ggtcttgaac
     7261 ttgatgacga cggcgaccgg gccgaagatc tcctcacggg cgaccttcat gtcgttggtg
     7321 acgtcgacga ggagcgtcgg ctgcatgaac gcaccctttt cagaggcgcg ggcgccgccc
     7381 acgaggacgc gcgcaccctc gctgacgcct tcctcgacgc agccgaggat cttggagagc
     7441 tggcgttcgt tgatctgggc gcccatctgg gtcgacatgt cggtcggatc gcccaccttg
     7501 atcttcttga aggcctcggc gagttcgccc acgaacttgt cgtagatgcc ttcctgcacg
     7561 aagatgcggg agcccgcgca gcagacctga ccctggttga agaggatgcc gatctgggcg
     7621 ccttcgatcg ccttgtcgag ctgggcgtcg tcgaagatga tgttggcgga cttgccgccg
     7681 agctcgagcg tgctcggaat caggcgcttg gcggccgctt cggcgacgct gtagccgact
     7741 tcggtcgagc ccgtgaaggc gagcttgtcg aagcccgggt ggtcaagcat ggcctggccc
     7801 gtgcgggcgc cgcggccgga gacgatgttg acgacgccgg cggggagcac ctggttgagg
     7861 agcttgccga gctcgaagag cgaaagcggc gtcgtcgagg aggacttgat cacgagcgtg
     7921 ttgcccgcgg cgagggccgg cgcgatcttc caggcgccca tcaggagcgg gaagttccac
     7981 ggaatgatct ggccgacgac gccgatgggc tcgtgcagca cgatgctcat cgtgttttcg
     8041 tcgatcatga cggcggagcc ctcttcggcg cgcaggcaac cggcgaagta gcggaagtgg
     8101 tcggaggaga gcggaacgtc gacgagcgtg gtttcgcgga tcggcttgcc gttgtcgagc
     8161 gtttcgacca tggcgagacg ggcggcgttt tcatcgatga gatcggcgac cttcagaaga
     8221 agggcggcgc gctcctgcgg ggacttggcg gcccaggcgg ggaaggcggc gcgggcggcg
     8281 gcgacggcac ggtcgatgtc ggcgggagag gcgttggcgc agtcggcgag cttctcgccg
     8341 ttggcgggat tgaacgtctc gaaggtttcg ccgccttcgg cgggaaccca ttcgccgttg
     8401 atgagcagat cgtacttggc gtcaatgtat tgcattttag gagcctccat gggcaagttc
     8461 gttcaaggag tcggcggaac tgctcggatg ccttctatca ataggcaacg tctaaaacga
     8521 aatagttctc aataaccgag atgtgaattc agtttatgag gaatttttct tgctgtcatt
     8581 gaccgaaggg ggtgttttgg gacttcattg gaactagtac caagggcgta agacgaaatt
     8641 caaatgtttc gaaatgcctg atgttcagtg gcttgtgagg aaaagcagga gggtgacgag
     8701 ggaggcgcac ttttgggcgc caccgtgcaa ggatgtataa aaaaatctat ggttgttgag
     8761 agaggctggg ggcgggcttt gcgtcgcccg atattcggaa ttttataaag tcgagccgag
     8821 aaaacccgcg gttttaaccg ccgggatgat aggcgctcaa atccccttcg cgcaaactcg
     8881 tgcgaagcat gcgtcctcga aaaaggacgg tgtaggattg aagtcatgaa agccaacatc
     8941 gcctacaagt tcgaactgat gcctacccgg gagcagaagc gaatcttcgc tcaagctgcc
     9001 gggtggcggc gtttcgtgtg gaacaaggcg atagaactgc agaagcggtg ccttgaacaa
     9061 gaggttcctt ttctcaagta cacggagctg gctggacggt tggtgctgtg gaaaaaggaa
     9121 gacgacatgt tgtggttgca ggaagcgcct tctcaggcac ttcaacagtg ccttcaacag
     9181 tgcctgatgg acgcgtctgc tgcaatcgaa gcgttcaagg ccaagaaaaa gggcttccca
     9241 aagttccaag ctcggggcga cggggattcc ttccgcattc cgcattccgc aagttcgccc
     9301 gacggattgg gacgaagcca acggtcgagt ccgtatcccg aaggcaggct tcgtgcgatt
     9361 cagaaagtcg cgtccgattc aaggcaagat caagcagatg acggtttccg tcgaatgcgg
     9421 gcattggttt gtctcggtcg tgacggagat ggatttcgcc aacgacggtc cgcgtcgtcc
     9481 cggcgag
//
LOCUS       DBMDTI010000055         9317 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2066_length_9317_cov_3.034288, whole genome shotgun sequence.
ACCESSION   DBMDTI010000055 DBMDTI010000000
VERSION     DBMDTI010000055.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9317)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2066_length_9317_cov_3.034288"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="ACZPB6_04205"
     CDS             <1..418
                     /locus_tag="ACZPB6_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024855.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HIR1183708.1"
                     /translation="VEAAPAPEQAAADQTAQEKTAAEQAAAEKAAAEKAAAEKAAAEK
                     AAAEKARAQSQAAATGERRVTLSTSGACWVQILLPNGRSFFAKEMAEGGSETLNVPVG
                     ARVTVGNASVMTLTVDGRPYELTKSTRNGICRFVVK"
     gene            502..1764
                     /gene="ispG"
                     /locus_tag="ACZPB6_04210"
                     /gene_synonym="gcpE"
     CDS             502..1764
                     /gene="ispG"
                     /locus_tag="ACZPB6_04210"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR1183709.1"
                     /translation="MSENTLEFRRKTHAVKVAWGGRVVTIGGMNPIVVQSMTNTSTAD
                     VESSVAQVLALARAGSELVRLTVNTPDAARGVARIREALDRSGCDVPLVGDFHYNGHK
                     LLADHPECAAALSKYRINPGNVGKGEKGMENFASMIECAKKYGAAVRIGVNWGSLDQA
                     LLARMMDENNALSDPLPPDAVLRAALVESAVQSADMAVKLGLPADRIVLSAKVSEVQG
                     LISVYRMLARRGSYALHLGLTEAGIGSKGIVASTAALAVLLQEGIGDTIRVSLTPSPG
                     APRTEEVVVAQEILQCMGLRSFTPMVTSCPGCGRTTSDLFQRLAAGTQDYLRSRMPEW
                     RVSCPGVENMTVAVMGCVVNGPGESRQADIGISLPGSGESPVAPVYVRGEKVCALKGD
                     DMQARFHAMIEEFVQTTYGASSRGQSDN"
     gene            1770..3056
                     /gene="hisS"
                     /locus_tag="ACZPB6_04215"
     CDS             1770..3056
                     /gene="hisS"
                     /locus_tag="ACZPB6_04215"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR1183710.1"
                     /translation="MSKEKFAGVKGMNDMLPADAAVWQTLQQTAVGVLERYGYQEIRT
                     PILENTKVFTRGVGEVTDIVEKEMYSFTDSMNGDQLTLRPECTSAVVRSVNEHSLLYG
                     ATQRLWYCGPMFRHERPQRGRYRQFHQLGAEALGMEGPDIDAEMIMMLARIWKELGVG
                     DVRLELNTLGALPERQAHREALIKYFEGNQDVLDEDARRRLYTNPLRILDTKNPEMQE
                     LVNAAPRLLDFLGEESRAFLARMEQLVSAAGIEYVINPRLVRGLDYYNHTVFEWITDK
                     LGAQGTICGGGRYDPLIEMLGGRPAPGVGFAMGMERVIELMRECGRVPVRTQTDVYVI
                     HSGGDTQVQAMLLAEALRDAGVRVMVHPGKSGFKSQMKKADASGARFAVFAAEDELAA
                     GTVSVKALREGEKSYAEQTALPLAGAAAAIAAALAA"
     gene            3130..3732
                     /locus_tag="ACZPB6_04220"
     CDS             3130..3732
                     /locus_tag="ACZPB6_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0044877 - protein-containing complex
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HIR1183711.1"
                     /translation="MKAWWDQWGTPITAAVCVCCLGFAGWNGWQWYQRNQAAQASGAY
                     VQLQNAIYQNDTKNVKSIADGLIEDYGTTIYAPLGALASAAAEVQEGNLDLARDRLVW
                     VIEKSGHPEYDTIARVRLAGVELSAKKPEAALKALEPAKPVPDQTALVEDRLGDVYYA
                     MNDFEKARAAWQKAVEAAGEQSPIRGVLHYKLASLPPVAE"
     gene            3915..5048
                     /gene="bamB"
                     /locus_tag="ACZPB6_04225"
     CDS             3915..5048
                     /gene="bamB"
                     /locus_tag="ACZPB6_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HIR1183712.1"
                     /translation="MFAKKALLGAAAALTVLTLAGCSTPDSHEPADLQDFREMVSPKV
                     SWTASVGESDGYLTPAVTDNAVYAAGGSSLYRLDRHTGDKVWEAEAGSRIVAGVGTDG
                     MHQAVVTDRGEVEVYDAEGKFIWRATLSAETDVPPLIGQGLVVVKTSDTRITAFDIVT
                     GERRWHYQGQAPALTLQAFNQMAWSPAGILAGQANGRLLALGLNGKPVFDAVIGQAAG
                     ITEVERLIDVVGRPWVDNQLMCAAAFQGNLVCMSSTNGQLVWNAKVDSVTGPVADARM
                     VYVVDDQSYVHAYNRANGREAWVNRDLEYRTVSAPIRIGATVAVADYEGYVTMMNPAN
                     GEVVGRTRLDGAVRAPAAQFGYGAVFQTVEGEVAYVLQESLGE"
     gene            5139..6485
                     /gene="der"
                     /locus_tag="ACZPB6_04230"
     CDS             5139..6485
                     /gene="der"
                     /locus_tag="ACZPB6_04230"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR1183713.1"
                     /translation="MLPVIALVGRPNVGKSTLFNRLTRSRDAIVADFPGLTRDRQYGQ
                     GRVGRMPYIVVDTGGFEPVKSEGIVKAMASQAELAIDEADVVIFVCDARSGLTPHDQR
                     IAVRLRQSGRKVILVVNKAEGLSAVAAAEFYELGFGEPWLISAAHGQGVNAVMDLALE
                     GFEEEPEDEEDDDPDAPKRLKVTLAGRPNAGKSTLINALIGEERLIAFDMPGTTRDAI
                     RVDFEYNGRPYELVDTAGLRKKGKVFEAIEKFSVVKTLQAIEDANVVILVVDAKNGIA
                     DHDAHIAGYVLDSGRALVVAVNKWDLMSDYDREMFNLELERKLHFLRWARMIRISALK
                     RNGLNHLMKAVDEAHAAAYRKIPTPKLTRALFEAVERQQPPRARGGRPKPRFAHQGGS
                     NPPVIVIHGNALEDIGESYRRYLEGFFREKFDLAGTPLRIEFRTKENPFVKDDNKR"
     gene            6791..7024
                     /gene="hfq"
                     /locus_tag="ACZPB6_04235"
     CDS             6791..7024
                     /gene="hfq"
                     /locus_tag="ACZPB6_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR1183714.1"
                     /translation="MTQKGQLLQDPFLNILRKDRIPVSIYLVNGIKLQGQIESFDQYV
                     VLLRNTVTQMVYKHAISTVVPTRAVNMPSVPEN"
     gene            7059..8258
                     /gene="hflX"
                     /locus_tag="ACZPB6_04240"
     CDS             7059..8258
                     /gene="hflX"
                     /locus_tag="ACZPB6_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR1183715.1"
                     /translation="MKATRAFLVCVVVGRVVYQDSAEELALLVKSDGLEPVGLLDAKR
                     DKPDPAFFIGSGKVDELGELAHELHADVVVFDVPLSAAQQRNIERAIELPVLDRTQLI
                     LDIFRARAKSREGRLQVELAQLEHLSTRLVRGWTHLERQRGGLGKTGGPGEKQIELDR
                     RMIGVRMKQLRDQLKRLARQRDTQRRSRSRSDTLSVSLVGYTNAGKSTLFNRLTRAKS
                     YAADQLFATLDTTARRCWISDEETVVASDTVGFIRGLPHQLVEAFKSTLDETVHADLL
                     LHVVDASSSVREDQISEVNAVLEEIKADDVPAILVYNKIDNTALEPEIVRHPDGRPKA
                     VFVSALTGEGLDLLRQAIGEFAHQWRVDNPNEPRAPEPWEEEVAHDLVALADDNIEDF
                     LVRKKKR"
     gene            complement(8328..>9317)
                     /locus_tag="ACZPB6_04245"
     CDS             complement(8328..>9317)
                     /locus_tag="ACZPB6_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1183716.1"
                     /translation="LEGGPMKNSLVIGAAGQIGSELVPELRRRGYMTVAAGSGRTPLT
                     AALSEGGPTAVVNLLDADGLRRTVEHYAIDTIFNMAAILSAVGESKPMTAWDIGVNGV
                     IGLLELARDKNLSVFTPSSIGVFGPETPHDPTPQDTVCRPRTMYGITKVAGELLSDYY
                     RAKYGVDARSVRYPGLISHVTPPGGGTTDDAVDIYWKAVEHGRYASPLAEGTRLPMMF
                     MADALRAAIELMEADASRLRHFNSFNVGAMSFAPEDVAASIRRRMPGFELTYEVNPLL
                     QGIAESWPDALDDTTAREEWDWKPEYDLDAMTDVMLEAISERLGGKSGHSSQA"
BASE COUNT         1650 a         3268 c         2832 g         1567 t
ORIGIN      
        1 agttgaagcg gcgcccgccc ctgaacaggc ggcagccgat cagactgctc aggagaagac
       61 tgcggccgaa caggccgccg ccgagaaggc cgctgccgag aaggccgctg cggagaaggc
      121 cgccgctgaa aaggcggccg ccgaaaaggc ccgcgcccag tcgcaggccg ccgcgacggg
      181 tgagcgccgc gtcaccctct cgacctcggg cgcctgctgg gtgcagatcc ttctgcccaa
      241 cggccgcagc ttcttcgcca aggagatggc cgagggcggc agcgagaccc tgaacgtgcc
      301 cgtcggggcc cgagtgacgg tcggcaacgc ctccgtcatg acgctcacgg tcgacggcag
      361 gccttacgaa ctcaccaagt ccacccgcaa cggcatctgc cgcttcgtgg tgaagtaaga
      421 agattcaaca gagcagcggc cttcgggccg ctcttttcac accggcctcc ggccggtgcg
      481 tatttcccgg agacttaagc cgtgtcggaa aacacccttg aattccgtcg caagacccac
      541 gccgtcaagg tcgcctgggg cggccgcgtc gtgacgatcg gcggcatgaa cccgatcgtc
      601 gtgcagtcga tgacgaacac ctcgacggcc gacgtcgagt cgagcgtcgc ccaggtgctt
      661 gcgcttgcgc gcgcgggcag cgagctcgtc cgactcaccg tcaacacgcc cgacgccgct
      721 cgcggcgtcg cccgcattcg cgaggcgctc gaccgctccg gctgcgacgt cccgctcgtg
      781 ggcgacttcc actacaacgg ccacaagctg ctcgcagatc atcccgaatg cgccgccgcg
      841 ctctccaagt accgtatcaa tcccggcaac gtcggcaagg gcgagaaggg catggagaac
      901 ttcgcctcca tgattgagtg cgcgaagaag tacggcgccg ccgtacgcat cggcgtcaac
      961 tggggctcgc ttgatcaggc gctcctcgcc cgcatgatgg acgagaacaa cgcgctctcc
     1021 gatccgcttc ctcccgacgc cgtccttcgc gccgctctcg tcgagagcgc cgtgcagagc
     1081 gccgacatgg ccgtgaagct cggccttccc gccgaccgca tcgtgctgag cgccaaggtc
     1141 tccgaagtcc agggcctcat ttccgtctac cgcatgctcg caaggcgcgg cagctacgcg
     1201 ctccacctcg gccttaccga ggcgggcatc ggctccaagg gcatcgtcgc ctcgacggct
     1261 gcgctcgccg tgctccttca ggaaggcatc ggcgacacga tccgcgtctc gctcacgccg
     1321 tctcccggcg cgccccgcac cgaggaggtc gtggtcgccc aggagatcct gcagtgcatg
     1381 ggcctcagga gcttcacccc gatggtgacg agctgccccg gctgcggtcg tacgacgtcg
     1441 gacctcttcc aacgccttgc cgcgggcacg caggactacc tgcgctcgcg catgcccgag
     1501 tggcgcgtct cgtgccccgg cgtcgagaac atgacggtgg ccgtcatggg ctgcgtcgtc
     1561 aacggtcccg gcgagagccg tcaggccgac atcggcatca gcctgccggg ctccggtgag
     1621 agccccgtgg ctcctgtcta cgttcgcgga gagaaggtct gcgccctcaa gggcgatgac
     1681 atgcaggccc gctttcacgc tatgattgaa gaattcgtcc aaacgaccta cggcgcatcg
     1741 tcccgtgggc aatccgataa ttgacaagaa tgagcaaaga aaaattcgcc ggcgtcaagg
     1801 gcatgaacga catgctcccc gccgatgccg ccgtctggca gaccctgcag cagacggccg
     1861 tcggcgttct cgaacgctac ggctatcagg aaatccgcac tccgatcctt gaaaacacga
     1921 aggtgttcac ccgcggcgtc ggcgaagtga ccgatatcgt ggagaaggag atgtactcct
     1981 tcaccgacag catgaacggc gaccagctca cgctgcgtcc cgaatgcacc tccgccgtcg
     2041 tgcgctccgt caacgagcac agcctgctct acggcgccac gcagcgcctc tggtactgcg
     2101 gcccgatgtt ccgtcacgag cgtccgcagc gcggccgcta ccgccagttc caccagctcg
     2161 gcgccgaggc gctcggcatg gagggtccgg acattgacgc cgagatgatc atgatgctcg
     2221 cccgcatctg gaaggaactc ggcgtgggcg atgtccgcct cgagctcaac acgctcggcg
     2281 cgctgcctga acgccaggcc caccgcgagg ccctgatcaa gtacttcgag ggcaatcagg
     2341 acgtgctcga cgaggacgcc cgccgccgtc tctacacgaa tccgctgcgc attctcgaca
     2401 cgaagaaccc cgagatgcag gagctcgtca acgctgctcc gcgtctgctc gacttcctcg
     2461 gtgaggaaag ccgcgccttc ctcgcccgca tggagcagct cgtctccgcc gccggcatcg
     2521 agtacgtgat caatccccgc ctcgtgcgcg gcctcgacta ctacaaccac acggtcttcg
     2581 agtggatcac ggacaagctc ggcgcccagg gcacgatctg cggcggcggt cgctacgacc
     2641 cgctcattga aatgctcggc ggtcgtcccg cccccggcgt gggcttcgcc atgggcatgg
     2701 agcgcgtcat cgaactcatg cgtgaatgcg gccgcgtgcc ggtccgcacc cagaccgacg
     2761 tctacgtcat ccattcgggc ggcgacacgc aggtccaggc gatgcttctc gccgaagcgc
     2821 ttcgcgacgc gggcgtgcgc gtgatggtgc accccggcaa gtccggcttc aagagccaga
     2881 tgaagaaggc cgacgcgagc ggcgcccgct tcgcggtctt cgccgccgag gacgagcttg
     2941 ccgccggcac ggtctccgtc aaggcccttc gcgagggcga gaagtcctac gccgagcaga
     3001 cggccctgcc gctcgccggc gccgccgcgg ccattgccgc cgcgctcgcc gcgtaatcat
     3061 tcaatcgagt tgacttaagg agcgcaagcg atggcttacg acttggaaga acaagagagc
     3121 attgaccaaa tgaaggcctg gtgggatcag tggggcaccc cgatcaccgc cgccgtctgc
     3181 gtctgctgcc tcggctttgc cggctggaac ggctggcagt ggtaccagcg caatcaggcc
     3241 gcacaggcgt ccggcgccta cgtgcagctg cagaacgcga tctatcagaa cgatacgaag
     3301 aacgtgaagt cgatcgccga cggcctcatc gaggactacg gcacgacgat ctacgcgccc
     3361 ctgggcgcgc tcgcctccgc cgcggccgaa gttcaggagg gcaatctcga cctcgcccga
     3421 gaccgtctcg tctgggtgat cgagaagtcg ggccatcccg agtacgacac catcgcgcgc
     3481 gttcgcctcg cgggcgtcga gctctcggcc aagaagcccg aggccgccct caaggcgctt
     3541 gagcccgcca agcccgttcc cgaccagacc gctctggtcg aggaccgcct cggcgacgtc
     3601 tactacgcca tgaacgactt tgaaaaggcc cgcgccgcct ggcagaaggc cgttgaggcc
     3661 gctggcgagc agagcccgat ccgcggcgtc ctccactaca agctcgcctc gcttccgcct
     3721 gttgcggaat gacggactgc gccgcccggc acggccgtga ccgggcagga ggaccctgca
     3781 tgacggcggc gcaggacgaa tgaaaaatac gagtcatttg tccccgctgc cgttcttttt
     3841 gctaatacaa tcatggattc agccgcgcgg tggtttgcct gcggcgagaa gcccaacaca
     3901 acgagatgaa tacgatgttt gccaagaaag ctctgctcgg cgccgccgca gcgttgacgg
     3961 ttctcaccct cgccggctgc agcacgcccg acagccacga acctgcggac ctgcaggact
     4021 tccgtgaaat ggtcagcccg aaggtctcct ggacggcttc cgtcggcgaa agcgacggct
     4081 atctcacgcc tgccgtgacg gacaatgccg tctacgccgc gggcggcagc agcctctacc
     4141 gccttgaccg ccatacgggc gacaaggtct gggaagccga agccggctcc cgcatcgtcg
     4201 caggcgtcgg cacggacggc atgcatcagg ccgtcgtaac ggaccgcggc gaagtcgagg
     4261 tctacgacgc cgagggcaag ttcatctggc gtgccacgct ctccgccgag acggacgttc
     4321 cgccgctcat cggccagggc ctcgtcgtgg tgaagacctc cgacacgcgc atcaccgcct
     4381 tcgacatcgt caccggcgaa cgccgctggc actatcaggg ccaggctccc gcgctcacgc
     4441 tccaggcctt caaccagatg gcctggtccc ccgcgggcat cctcgccggt caggccaacg
     4501 gccgtcttct cgccctcggc ctcaacggca agcccgtctt tgacgccgtc atcggtcagg
     4561 ccgccggcat caccgaggtc gagcgcctca tcgacgtcgt cggtcgtccg tgggtcgaca
     4621 accagctcat gtgcgccgcc gccttccagg gcaacctcgt gtgcatgagc tcgacgaacg
     4681 gtcagctcgt ctggaatgcg aaggtcgaca gcgtcaccgg tccggtcgcc gacgcccgca
     4741 tggtctacgt cgtcgacgac cagagctacg tccacgccta caaccgcgcc aacggccgcg
     4801 aagcctgggt caaccgcgac ctcgagtacc gcacggtctc cgctccgatc cgcatcggtg
     4861 ccacggtggc cgttgccgac tatgagggct acgtcacgat gatgaacccc gccaacggcg
     4921 aggtcgtcgg ccgcacccgc cttgacggcg ccgtgcgcgc gcccgccgcc cagttcggct
     4981 acggcgcggt cttccagacc gtcgaaggcg aggtcgccta cgtgcttcag gagtcgctcg
     5041 gcgaatgacg ccgcgcgccc gcgctcttta aaacgccttg aatccacgcc ggtcgatgct
     5101 cctctggagc gtcgtccggc ctctcttttt ccagaaacat gcttcctgtc attgctctcg
     5161 tgggccgtcc gaacgtcggc aagtccacgc ttttcaatcg cctcacgcgc agccgcgacg
     5221 ccatcgtcgc cgacttcccc gggcttaccc gcgaccgtca gtacggtcag ggccgtgtcg
     5281 gccgcatgcc ctacatcgtc gtcgacacgg gcggcttcga gcccgtcaag tccgaaggca
     5341 tcgtcaaggc catggccagc caggccgagc ttgcgatcga cgaggccgac gtcgtcatct
     5401 tcgtctgcga cgcccgttcc ggcctcactc cgcacgacca gcgcatcgct gttcgcctgc
     5461 gccagtccgg ccgcaaggtg atcctcgtcg tcaacaaggc cgaaggcctt tccgctgtcg
     5521 ccgccgccga gttctacgag ctcggcttcg gtgagccgtg gctcatctcg gccgcccacg
     5581 gccagggcgt caacgccgtc atggacctcg ccctcgaggg cttcgaggag gagcccgagg
     5641 atgaggagga tgacgacccc gatgcgccca aacgcctcaa ggtgacgctc gccggccgtc
     5701 cgaacgccgg caagtccacg ctcatcaacg ccctgatcgg cgaagagcgc ctcatcgcct
     5761 tcgacatgcc gggcaccacc cgcgacgcca tccgcgtcga cttcgaatac aacggccgtc
     5821 cctacgagct cgtcgacacg gccggccttc gcaagaaggg caaggtgttc gaggccatcg
     5881 agaagttctc cgtcgtcaag accctgcagg ccatcgagga cgccaacgtc gtcattctcg
     5941 tcgtcgacgc caagaacggc atcgccgatc atgacgccca cattgcgggc tacgtactcg
     6001 actcaggccg cgccctcgtc gtcgccgtca acaagtggga cctcatgtcc gactacgacc
     6061 gcgagatgtt caacctcgag ctcgagcgca agctgcactt cctgcgctgg gcccgcatga
     6121 tccgcatctc ggccctcaag cgcaacggcc tcaatcacct catgaaggca gttgacgagg
     6181 cccacgccgc ggcctaccgc aagattccga cgcccaagct cacgcgagcc ctcttcgagg
     6241 ccgtcgagcg ccagcagccc ccgcgcgccc gcggcggccg tccgaagccc cgcttcgcgc
     6301 accagggcgg ctcgaatccg ccggtcatcg tgattcacgg caacgccctc gaggacatcg
     6361 gcgaaagcta ccgccgctat ctcgaaggct tcttccgcga gaagttcgac ctcgccggca
     6421 cgccgcttcg catcgagttc cgcacgaagg aaaacccctt cgtcaaggac gacaacaagc
     6481 gctgatcctc tcgggatccg ggggcggccg tcgcccccgc gtgcacgaaa acgctcccct
     6541 cgaatctggc gggagcgttt ttttcgccgc cgtcttttct gatgcccgtg aaaacgcgtt
     6601 tttcggtttc tttttctctt tgcggacgat atttacaatc gtcaggtgat aagatagaag
     6661 ggagtgccgc agtacgtgcg gccgtgcgcg ggcggttgcc ggaaccggtt cagatccacc
     6721 cgcatcgaat aacaacaaag actgatgggc tgccgcaagg aacaggccca gacggtcgga
     6781 gaacaacaaa atgactcaga aaggtcagct tcttcaggat ccgttcctga acatccttcg
     6841 caaggatcgc atccccgttt ccatttatct cgtcaacggc atcaagcttc agggccagat
     6901 cgaatccttt gaccagtacg tcgtgcttct gcgcaacacg gtcacccaga tggtctacaa
     6961 gcatgcgatc agcaccgtcg ttccgacgcg tgccgtgaac atgccctccg tgccggagaa
     7021 ctgagataag cacgttccag atcgatgaga cgcgcgcggt gaaggccacg cgcgcctttc
     7081 tcgtatgcgt cgtcgtcggc cgcgtcgtct accaggactc ggccgaagag cttgcgcttc
     7141 tcgtcaagtc cgacggactc gaacccgtcg gacttcttga tgccaagcgc gacaagcccg
     7201 atcccgcctt cttcatcggc tcgggcaagg ttgacgagct cggagagctc gctcatgagc
     7261 tgcatgccga cgtcgtcgtc ttcgacgtcc ccctctcggc ggcccagcag cgaaacatcg
     7321 agcgcgccat cgagcttccc gttcttgacc gcacccagct gattctcgac atcttccgcg
     7381 cccgcgcgaa gtcccgcgaa ggccgcctgc aggtcgaact cgcccagctc gagcatctct
     7441 ccacgcgcct cgtgcgcggc tggactcatc tggagcgcca gcgcggcggc ctcggcaaga
     7501 ccggcggccc cggcgaaaag cagatcgagc ttgaccgccg aatgatcggc gtgcgcatga
     7561 agcagcttcg cgaccagctc aagcgtctcg cgcgtcagcg tgacacgcag cgccgctcgc
     7621 gctcccgctc cgacacgctc tccgtttcgc tcgtgggcta cacgaacgca ggtaagtcga
     7681 cgctcttcaa ccgcctcacg cgcgccaaga gctacgcggc cgaccagctg ttcgcaacgc
     7741 tcgacaccac cgcccgccgc tgttggatca gcgacgagga gacggtcgtc gcgagcgaca
     7801 ctgtgggctt catcaggggc cttccgcatc agctcgtcga ggccttcaag tccacccttg
     7861 acgaaacggt ccatgcggat cttctgcttc acgtggtcga cgcctcctcg tcggtccgag
     7921 aggaccagat cagcgaggtt aacgcggtgc tcgaggagat caaggcggac gacgtccccg
     7981 cgatcctcgt ctacaacaaa atcgacaata cggctctcga acccgagatc gtgcgtcatc
     8041 ccgacggccg gcccaaggcg gtcttcgttt ccgcccttac gggtgagggg ctcgatcttc
     8101 tgcgtcaggc gatcggcgag ttcgcgcacc agtggcgcgt tgacaatccg aacgaaccgc
     8161 gcgcgcccga accctgggag gaagaggtcg cacacgacct tgtcgccttg gcggacgaca
     8221 acatcgagga cttcctcgtt cgcaagaaga agagatagcc gctgacgccc ttctgcgcct
     8281 tcatgcaaga aagcctgcat tcgcgatgaa tgcaggcttt ctgttcatca ggcctgagag
     8341 ctgtgccctg actttccgcc gagcctttcg gagatcgcct cgagcatgac gtccgtcatt
     8401 gcgtcgaggt cgtactcggg tttccagtcc cattcctcgc gcgccgtcgt gtcgtcaagt
     8461 gcgtcaggcc agctctcggc gatgccctgc agcaggggat tcacctcgta ggtgagctcg
     8521 aacccgggca tccgccttct gatgctcgcc gccacgtcct cgggcgcaaa gctcatcgcg
     8581 cccacgttga agctgttgaa gtgccggagt cttgaggcat cggcctccat cagctcgatg
     8641 gcggcgcgca gcgcatcggc catgaacatc atcggcagcc tcgtcccttc ggcgagcgga
     8701 gaggcgtagc ggccgtgctc gactgccttc cagtagatgt cgaccgcatc gtcggtcgtg
     8761 ccgccgccgg gcggcgtcac gtgggagatg aggcccggat acctgacgct tcgggcgtcg
     8821 acgccgtact tcgccctgta gtagtcggag agcagctcgc ctgccacctt cgtgatgccg
     8881 tacatcgtcc tcggcctgca gaccgtgtcc tgaggcgtcg gatcatgggg cgtttcggga
     8941 ccgaagaccc cgatggagct cggtgtgaag acggagaggt tcttgtcgcg tgcgagctcg
     9001 agaagaccga tcacgccatt gacgccgatg tcccatgccg tcatgggctt cgactcgccc
     9061 acggccgaga gaatggccgc catattgaag atcgtgtcga tggcgtagtg ttcgacggtc
     9121 ctgcgcaggc cgtccgcgtc gagaaggttg acgacggccg tcggaccgcc ttcggagagc
     9181 gcggcggtca gcggcgtgcg accgctgcct gcggccacgg tcatgtagcc gcggcgcctg
     9241 agttcgggga cgagctcgga gccgatctgg cctgccgcgc cgatcacgag gctgttcttc
     9301 atgggccctc cttccag
//
LOCUS       DBMDTI010000056         9290 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2074_length_9290_cov_3.972358, whole genome shotgun sequence.
ACCESSION   DBMDTI010000056 DBMDTI010000000
VERSION     DBMDTI010000056.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2074_length_9290_cov_3.972358"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(181..693)
                     /gene="secB"
                     /locus_tag="ACZPB6_04250"
     CDS             complement(181..693)
                     /gene="secB"
                     /locus_tag="ACZPB6_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HIR1183717.1"
                     /translation="MAENENAQHQDNQQPQQEAAPEPVFQLQRCYLKDASLEMPHAPE
                     ILLQAQQEQPQVDIKFEVSQKTVAEALYDVTVRGTITVKAADKVIILVEGTQSGLFAI
                     HGIPVEALQHVTNVVCPAMVYPYLRANLADLMNRANVPPVHLPEVNFEVLYQQRLAQA
                     QAEAEGAAQA"
     gene            complement(806..1207)
                     /locus_tag="ACZPB6_04255"
     CDS             complement(806..1207)
                     /locus_tag="ACZPB6_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR1183718.1"
                     /translation="MSQFLIDNSLLIVVIAVSVLGLLIPVINTRRYAPEVSPAQATEL
                     INRQGAQIVDVRKAADFAKGHIANSRNIPADQIQNEFGKLKRERPVILVDQTGAGSRP
                     VARLLRGVGFQRVTILERGIVGWLQAKMPLE"
     gene            1415..2677
                     /locus_tag="ACZPB6_04260"
     CDS             1415..2677
                     /locus_tag="ACZPB6_04260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR1183719.1"
                     /translation="MKPFRLKFLCTACLAGALWLSPMHDGSDALAASAQQKAAQEKKR
                     VEGQRANIEQKLQSLQAELDAREARSEEASQALKTADQAISQANRKLRDLREEKRGVE
                     DELAKLRKSGNNVADNLKDAENTVAQIAKAQYINSRRSHWQNLIGGINPSTLASDKGK
                     LEYLARTQQAAIDDLEERQSNIRNLSEKHLKQHHELARIQQEEEKERKSLLKEKQERQ
                     QAYGKLQSEIKSRQARIDKLKKDQARLSSLVAAIDARLEKERRQAEQVAKKQAIRPPS
                     QKQNASYAPQVGSFGKLKGRLTRPVKGTVVGRFGTERQDQPGTTWQGMQFRAQEGSEV
                     SACAAGKVVFSDWLRGFGNLIIIDHGNTYMSIYGNNESIFTNVGDTVKQGETISSVGI
                     SGGLGEPGLYFELRYKGKPINPQPWLKP"
     gene            2733..4262
                     /locus_tag="ACZPB6_04265"
     CDS             2733..4262
                     /locus_tag="ACZPB6_04265"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR1183720.1"
                     /translation="MHKLRSVTLLCAGICAGVLSTVGITAYAEKQSAAPLPLAEIRQF
                     TSAFNAIKDFYVDDVSDAKLLQSAVDGMVSGLDPHSSFLDVEGYKDMTESTQGSFGGL
                     GLEVTKDPAGVRVISPIDDTPAARAGMRAGDIITRIDGKSVADMSLNAAVKLMRGQPN
                     TKIELVVARKGANKPLNFSLVRALIKTQSVKMKKLDDKVGYIRISQFQERTAEDLSKY
                     LDELAKDGHLEGLVLDLRNDPGGLLQAAIGVSAAFLPKGSDVVSTKGRQAQSDYTFKA
                     VEADYRAGNAVRALSGLTPVAKKVPIVVLINSSSASASEIVAGALQDHKRATIMGDRS
                     FGKGSVQTIMPMKFGDKTVAVKLTTARYYTPSGRSIQAKGIIPDIYVDDTPKGNYPSF
                     QIREADLTHHLINQDENKDEKDLLKDLPYDDEEGEIPDYQYMFGDDKDWQLQQAVNFL
                     KNKPVAESKYRGKPRDLVKKLKAEAAAEKLKANRAKASKMEKKAEDAKADKAEDAKTE
                     N"
     gene            complement(4333..4944)
                     /gene="slmA"
                     /locus_tag="ACZPB6_04270"
     CDS             complement(4333..4944)
                     /gene="slmA"
                     /locus_tag="ACZPB6_04270"
                     /inference="COORDINATES: protein motif:HMM:NF007015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HIR1183721.1"
                     /translation="MTDAVEVTLSHRRTKGERRQKVLQHLAGMLNEATLEKITTARIA
                     EVMDISEGLLYRYFKNKADMFDALIDFAEESLMGLTGQIRASEGMSGLDQVRASVNVM
                     LQFADANPGITRVMTGQVLIYEDPRLIERVSRLHLALEAGLKLSYRTAVAEGALPADF
                     DASGRASLLMASVLGQWQRFVLTGFKVRPGLKSSSDTELFLKP"
     gene            complement(4990..6768)
                     /gene="sppA"
                     /locus_tag="ACZPB6_04275"
     CDS             complement(4990..6768)
                     /gene="sppA"
                     /locus_tag="ACZPB6_04275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR1183722.1"
                     /translation="MFSRIAAAYALLDGVRRLLVNLLFLLTLVTLAVVFWVASEAPNL
                     PKGTLLRIDLVGEVKESGPAEPSFMLSMFGQDSFEENTRLADVVDALDRAAHDDRIAG
                     VVLRVDDLAGAGMASIREIGAAIDRYRQTSGHQVWTWSISYTQPQYLVAAHADHVGIH
                     PMGDAIVKGLSATTFYWGPLLNAAGLEVEVHKAGAFKSAPEIFTSGRPSAESLAAQKS
                     YMDDAWAGLVSRLEARRGLVNGTVEKFIAGIAKDGTAEKSLSVFFREAGLIDELETRD
                     AYLQKLADKYAGGQVKDLKAIDATAYLAATETVSAGNTVAVLIAEGEITGMPELGGMT
                     PDGINGVIDEIQEDPKVKALVVRVNSPGGDAVAAELIRARLVEYKKKTGNPVIISMGD
                     TAASGGYWIATAGDRIVADPLSITGSIGVFAMSVHAEGLREELKVGRGGYRTTPLADF
                     GNPAAAPGDVERRLIEGGVSRTYADFKKLVAESRGMSVEEVERVAQGRVWTGAQAVKF
                     GLADAQGGMNEALKLACREAKLPAETASVLWEPVDTSWRGALRAFLQSRAGAAVGKVL
                     EVRQALDALAAQSNRPLAWAPFEPTL"
     gene            complement(6731..7471)
                     /locus_tag="ACZPB6_04280"
     CDS             complement(6731..7471)
                     /locus_tag="ACZPB6_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HIR1183723.1"
                     /translation="MPKLWLFDLDDTLFEASGGMLHKIHLLMNEYMCRELGMEWEEAS
                     ALRRHYWSVYGATFLGLWRHHGIDPRDFLSFAHDFDPRLYIQFSGCPAEDVKRLPGRK
                     VVFTNGPRNYARAVLEALELDHVVDGLVASTDMHALGQWRPKPSRLMFLMTCRRWGVS
                     PADTVFVDDSPMNLMAAHAEGIRTVWCTGYRKKNGKLANRIVRPCADFTIGHIRELSR
                     LQFGTPDDRIFSNGCLNVLTHRRSLCAA"
     gene            complement(7604..8023)
                     /gene="dksA"
                     /locus_tag="ACZPB6_04285"
     CDS             complement(7604..8023)
                     /gene="dksA"
                     /locus_tag="ACZPB6_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HIR1183724.1"
                     /translation="MGTTKLLTVDELLKMSEDDYMDERQLAFFKNRLQTMEAELRANA
                     DQTTVNLRETTVVPDPADRATIEEEHALELRTRDRERKLLKKVQAAIKRIDDGEYGYC
                     EETGDPIGVARLLARPTATLSLEAQQRRELKQKLYGD"
     gene            complement(8189..9181)
                     /locus_tag="ACZPB6_04290"
     CDS             complement(8189..9181)
                     /locus_tag="ACZPB6_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR1183725.1"
                     /translation="MMVEEQQPDIPVTILTGFLGAGKTTLLNRILSENHNYKIAIVEN
                     EFGKENIDTDLLVQTDREQIVNMNNGCVCCTIRGDLSKVLTDLRLRRSRGEIKFDYVV
                     IETSGVANPGPVAQTFFMDDAVAAFFRLDGVVTVVDCKHGSATLDEEAAARDQVGFAD
                     RILLSKTDLCTAEEVAAMEQRLRQMNARAPIRRVNMGECPVDEVLNITGFNMNDVLDV
                     DPAFFNNDHHMHHHNDDIHSFVYESDRPFNPMKLERYIASLTSVYGQDMLRYKGVLYL
                     SGTDRRVVFQGVHMLAVSDVLDTWGDRKPFSKIVFIGRNLPEEAIKRGLATCLD"
BASE COUNT         1979 a         2926 c         2651 g         1734 t
ORIGIN      
        1 gttggtaaaa aaaacagcac ctcctggcgt cgttaagaaa aactgaacgg aacatttttg
       61 ttccatccgg cttggtaaca ggggtttttg aagagcttcc taagaacgcc tgctcgccaa
      121 cacctgaaaa aacgaaaggc agcccgaagg ctgcctttcg ccggcgaatc aaagtaccga
      181 tcaggcctga gcggcgcctt cggcttcagc ctgagcctgg gcgagacgct gctggtaaag
      241 aacttcaaag ttcacttcag gcagatggac cggcggaaca ttggcgcggt tcatcaggtc
      301 ggcgaggttg gcgcggaggt acggatagac catggccggg cagacgacat tcgtgacatg
      361 ctgcagggcc tcgaccggaa tgccgtggat ggcgaaaaga ccagactgcg tgccttcgac
      421 gaggataatc accttgtcgg cggccttgac cgtgatggtg ccgcggacgg tcacgtcgta
      481 aagggcttcg gcaaccgtct tctggctgac ttcaaacttg atgtcgacct gcggctgttc
      541 ctgctgggcc tgcagaagga tttcaggagc atgcggcatt tcaagggagg cgtccttgag
      601 gtagcagcgc tgaagctgga acacgggttc cggagcggct tcctgctggg gctgctgatt
      661 atcctggtgc tgggcgtttt cgttttctgc cattttggat tccttgcccg gcggcctcgc
      721 gggcaaccgg aattaaacaa ctgacgagaa tgcccggcga accctttcgt caggcacttt
      781 atttggatat ggggatgatt tcgaattatt caagcggcat cttggcctgc agccagccga
      841 cgatgcctcg ctctaggatg gtcacccgtt ggaagcccac gccgcgaagc agtcgggcga
      901 cgggacggga gcccgcgccg gtctggtcaa cgaggatcac ggggcgctcg cgcttgagct
      961 tgccgaattc gttctggatc tggtcggcgg gaatgttgcg gctgttggcg atgtgtccct
     1021 tcgcgaagtc ggccgccttg cggacgtcga cgatctgggc accctggcga ttgatgagct
     1081 cggtcgcctg agcgggcgag acttcgggag cgtagcggcg ggtgttgatg acgggaatga
     1141 gaagaccgag gacggaaacc gcgatcacga cgatcagcag gctgttgtca atgagaaact
     1201 gcgacatgtt tattgcgttc gattatggat cggaagcccc ctgatccccg cactctgacg
     1261 agtgccgccg gagcagaggc gaatccaatg attgtagacg agatgcacac tcatcgggct
     1321 cttttgccgc tatgatgagc atcatggaaa gcgcgaactt tccttttgtt ttgcttttgt
     1381 ttttgcctag ccgatccacg ccgtacttcg caccgtgaag cccttccgtc tgaaattcct
     1441 ctgcaccgcc tgccttgccg gcgcattgtg gctctccccg atgcacgacg gctcggacgc
     1501 gcttgccgcc tccgcccagc agaaggctgc ccaggaaaag aagcgcgtcg agggccagcg
     1561 cgccaacatc gagcagaagc tgcagagcct gcaggccgag ctcgatgcac gcgaagcgcg
     1621 cagcgaggag gccagccagg cgctcaagac cgccgatcag gccatctcgc aggccaaccg
     1681 aaagcttcgc gaccttcgcg aggaaaagcg cggggtcgag gacgagctag ccaagctccg
     1741 caagagcggc aacaacgtgg ccgacaatct caaggacgct gaaaacaccg tcgcccagat
     1801 cgccaaggct cagtacatca acagcaggcg cagccactgg cagaacctga tcggaggcat
     1861 caacccgagc acgctcgcca gtgacaaggg caagctcgag tatctggccc gcacgcagca
     1921 ggcggctatc gacgatcttg aagagcgtca gagcaacatc cgcaacctct ccgaaaaaca
     1981 cctcaagcag caccacgagc tcgcacgcat ccaacaggag gaggaaaagg agcgcaagtc
     2041 ccttctcaag gagaagcagg agcgccagca agcctacggc aagcttcagt ccgagatcaa
     2101 gtcgcgtcag gcccgcatcg acaagctcaa gaaggatcag gcgcgcctga gttcacttgt
     2161 ggccgcaatc gacgcgcgcc tcgaaaagga acgccgtcag gcggagcagg tcgccaaaaa
     2221 gcaggccatc cgcccgccgt cccaaaagca gaacgcctcc tatgcgccgc aggtcggcag
     2281 cttcggcaag ctcaagggac gcctgacccg tcccgtcaag ggcaccgtgg tcggccgctt
     2341 cggcaccgag cgtcaggatc agccgggcac cacctggcag ggcatgcagt tccgggcaca
     2401 ggaaggctcg gaggtttccg cctgcgcagc cggcaaggtc gtcttctccg actggcttcg
     2461 cggcttcggc aacctcatca tcatcgacca cggcaataca tatatgtcga tttatggcaa
     2521 caatgaatcg atcttcacga acgtgggcga caccgtcaaa caaggcgaaa caattagttc
     2581 cgtaggcatt tcgggaggcc ttggcgagcc gggcttatac tttgagctcc gctacaaggg
     2641 caagccgatc aatccccagc cctggctgaa accgtaaccc atcccgcccc atgaggaagc
     2701 cctcctctcc agtcgggcac ctaaaaatca tcatgcacaa gcttcgatcc gtcaccctgc
     2761 tgtgcgcagg catctgcgcc ggcgtcctct ccaccgtcgg cattaccgcg tacgccgaaa
     2821 aacagtcggc cgcgcccctg ccccttgcag agatccgcca gttcacgagc gccttcaatg
     2881 cgatcaagga cttctacgtt gacgacgtct cagacgccaa gctccttcag tccgccgtcg
     2941 acggcatggt gagcggtctc gatcctcact cgagcttcct cgacgtcgag ggctacaagg
     3001 acatgactga aagcactcag ggctccttcg gcggcctcgg gcttgaagtg acgaaggacc
     3061 ccgcgggcgt tcgcgtcatc tccccgatcg acgacacgcc cgccgcacgc gcaggcatgc
     3121 gtgcaggcga catcatcacg cgcatcgacg gcaagtcggt ggcggacatg tccctcaatg
     3181 cggccgtcaa gctcatgcgc ggtcagccca acaccaagat cgagctcgtc gtggcccgca
     3241 aaggcgccaa caagccgctc aacttctcgc tcgtccgagc cctcatcaag acgcagtccg
     3301 tcaaaatgaa gaagctcgac gacaaggtgg gctacatccg catctcacag ttccaggagc
     3361 gcacggccga ggatctctcg aagtatctcg acgaactggc caaggacggt catctcgaag
     3421 gcctcgtgct tgacctgcgc aacgacccgg gcggcctgct ccaggcagcc atcggcgtct
     3481 ccgccgcctt cctgccgaag ggctccgacg ttgtttccac gaagggacgt caggcgcagt
     3541 ccgactacac cttcaaggcg gtcgaagccg actaccgcgc gggcaacgcc gtcagggcgc
     3601 tctccggcct gacgcctgtc gcaaagaagg tgcccatcgt cgtcctgatc aactcctcgt
     3661 ccgcctccgc ctccgaaatc gtggcgggcg ccctgcagga ccacaagcgc gcgaccatca
     3721 tgggcgaccg ctccttcggc aagggctccg tgcagaccat catgccgatg aagttcggag
     3781 acaagaccgt cgccgtgaag ctcacgacag cacgctacta cacgccgtcg ggccgctcga
     3841 ttcaggccaa gggcatcatc cccgacatct atgtcgacga cactccgaag ggcaactacc
     3901 cctccttcca gatccgcgag gccgacctca cgcaccacct gatcaatcag gatgaaaata
     3961 aggatgaaaa ggacctgctc aaggatctgc cctacgatga cgaggaaggc gaaatccccg
     4021 actaccagta catgttcggc gacgacaagg actggcagct ccagcaggcc gtcaatttcc
     4081 tcaagaacaa gcccgtggcc gaatccaagt accgcggcaa gccccgcgac ctggtgaaga
     4141 agctcaaggc cgaagcggcg gccgaaaagc tcaaggctaa tcgagccaag gcctccaaga
     4201 tggagaagaa ggccgaggac gccaaagctg acaaagctga agacgccaag accgaaaact
     4261 aagccgcact gaccaaaaaa ggaggccgcg aaactatccg cgacctcctt tttccatctt
     4321 ctgtctccct gctcaaggct tcaagaagag ctccgtatcg gagctgctct tgagcccggg
     4381 gcgcaccttg aagcccgtca gcacaaaacg ctgccactgg ccgagcacgg acgccatcag
     4441 gagggacgcc ctgccggacg catcgaaatc ggcaggaagc gcaccttcgg caacggccgt
     4501 gcggtaactc agcttcagcc ccgcctcaag ggcgagatgc agtcggctga cgcgttcgat
     4561 gagcctggga tcctcgtaga tgagaacctg ccccgtcatg actcgcgtga tgccgggatt
     4621 ggcgtcggca aactgcagca tcacattcac gctggccctc acctgatcga ggccggacat
     4681 gccctccgac gcacggatct gccccgtcag ccccatcagg ctttcctcgg caaaatcgat
     4741 cagtgcgtca aacatgtccg ccttgttctt gaagtagcgg taaagaaggc cctcgctgat
     4801 gtccatcacc tcggcaatgc gcgctgtggt gatcttttca agcgttgctt cgttgagcat
     4861 gcctgcaagg tgctgcagga ctttctggcg gcgctctccc ttcgtgcgtc gatgggagag
     4921 cgtaacttcg acggcatccg tcatgatgtt ctttcttcta cagtgctgaa gtctcgggaa
     4981 agcgtaagtt tacagcgtcg gctcgaacgg agcccaggcc agcggtcggt tcgactgagc
     5041 ggcaagcgca tcaagcgcct gcctcacctc gaggaccttt cccacggccg cccccgccct
     5101 gctctgcagg aacgcacgca gcgccccccg ccaggacgtg tcgacaggct cccacagcac
     5161 ggatgcagtt tcggccggca gcttcgcctc acggcacgca agcttcaggg cctcattcat
     5221 tccgccctga gcatccgcca gaccgaactt gacggcctga gcacccgtcc agacgcggcc
     5281 ttgggcgacg cgctcgacct cctcaacgga catgccgcgg gactcggcca ccagcttctt
     5341 gaagtccgcg tacgtacggc ttacgcctcc ctcgatgagt ctgcgttcca catcgccggg
     5401 cgccgcagca ggattgccga agtccgcaag cggcgtcgtg cggtagccgc cgcgtcccac
     5461 cttcagctcc tcacgaagtc cctcggcgtg cacgctcatc gcaaagacgc cgatcgagcc
     5521 cgtgatggaa agcggatccg ccacaatgcg gtcccccgcc gtggcgatcc agtagccgcc
     5581 ggaagccgcg gtgtcgccca tcgagatgat gacagggttc cccgtcttct tcttgtactc
     5641 aaccagacga gcacgaatca gttccgccgc aaccgcatca ccgccgggac tgttgacgcg
     5701 cacgacgagg gccttgacct tgggatcctc ctgaatttca tcgatcacgc cattgatgcc
     5761 gtcgggcgtc atgccaccaa gctcgggcat gcccgtgatc tcaccctccg caatgaggac
     5821 ggccactgtg tttcccgctg aaacggtctc tgtcgcggcc agataggccg tggcgtcaat
     5881 ggccttgagg tccttcacct gaccgcctgc atacttgtcg gcaagcttct gaagataggc
     5941 atctcgcgtc tcaagctcgt cgatgagtcc tgcctccctg aagaaaacgg agagcgactt
     6001 ttccgccgtt ccgtccttcg caatcccggc gatgaacttc tcgaccgtgc cgttgaccag
     6061 accacgacga gcctcgagac ggctcacaag ccccgcccaa gcgtcgtcca tgtaactctt
     6121 ctgggctgca agactttccg ccgaaggcct gcccgacgta aagatctcgg gagcgctctt
     6181 gaaggcgccc gccttgtgca cctcgacctc aagccccgca gcgttcagaa gcggtcccca
     6241 gtagaacgtc gtggcggaaa gccccttgac gatggcatcc cccatcggat gaatgccgac
     6301 gtgatcggca tgcgccgcga ccagatactg cggttgcgtg tacgaaatac tccatgtcca
     6361 gacctgatgc ccgctcgtct gacgatagcg gtcaatcgcg gctccgattt cacgaatgga
     6421 ggccatgccc gctcccgcca gatcgtcgac acgaagcacg acgcccgcga tgcggtcgtc
     6481 atgcgccgcc ctgtcgagcg catcaacgac gtccgcaagc ctcgtattct cttcaaagga
     6541 atcctgcccg aacatggaca gcatgaacga cggctctgca ggaccgctct ccttcacttc
     6601 tccgaccagg tcaatgcgaa gaagcgttcc cttgggcagg ttcggcgcct ccgaggccac
     6661 ccagaagacg accgccagcg taacgagcgt caggagaaag agaaggttca ccagaagacg
     6721 gcgtaccccg tcaagcagcg cataggctgc ggcgatgcgt gagaacattc aaacacccat
     6781 tgctaaaaat tctgtcgtcc ggcgttccga actgaaggcg ggaaagctcc ctgatgtgcc
     6841 cgatcgtaaa gtccgcacag ggacgcacga tgcggttggc aagcttgccg ttcttcttgc
     6901 ggtaccccgt gcaccagacc gtccggatcc cctcggcgtg cgcagccatg aggttcatcg
     6961 gactgtcgtc aacaaagacc gtgtcggcgg gagaaacgcc ccagcgccgg cacgtcatca
     7021 gaaacatcag gcgactcggc ttcggacgcc attggcccag cgcatgcatg tcagtgctgg
     7081 ccaccaggcc gtccacaaca tggtcaagct ccagcgcttc aagcacggcc cgggcatagt
     7141 tgcgcggacc gttcgtaaag acaaccttgc gtccgggaag cctcttcacg tcctctgcgg
     7201 gacatccact gaattggatg tagagacgcg gatcgaaatc gtgcgcaaag ctcaggaagt
     7261 cccggggatc gatgccatga tgcctccaga gccccaggaa cgtggcgccg taaacgctcc
     7321 agtagtgccg gcgaagagcc gaagcctcct cccattccat gccgagctct ctgcacatgt
     7381 attcgttcat cagcagatga atcttgtgga gcatgccgcc cgacgcctca aagagcgtgt
     7441 catcgagatc aaatagccac aacttcggca taggtttcct tctgttcgag tcgcaaatac
     7501 aaaaaaactc ggcaaccgtc cgtcatttta cggacaacga ttgccgagtt catgggagcg
     7561 cccgggtttc gggcgcatcc agcagggttg actccccgca gcatcagtct ccgtagagct
     7621 tctgcttgag ttcgcgacgc tgctgggcct cgagagaaag cgtggcggtc gggcgggcga
     7681 gcagacgggc gacgccgatg ggatcaccgg tttcctcgca atagccgtat tcgccgtcgt
     7741 cgatgcgctt gatcgcagcc tgaaccttct tcagaagctt gcgctcgcgg tcacgggtac
     7801 gcagttcaag cgcgtgctcc tcttcgatcg tggcgcggtc ggccggatcg ggcacaaccg
     7861 tcgtttcgcg aagattcacc gtcgtctgat cagcatttgc acgaagctcc gcttccatcg
     7921 tctgaaggcg gttcttgaaa aatgcaagct ggcgctcatc catgtagtcg tcttccgaca
     7981 tcttcaggag ctcgtcaacc gtcaaaagtt tagtcgtgcc cataacttct cgtctttgta
     8041 tttagattgg cattgtgtgg ccgaacaaag cggtgcggcc gtcagaaata tggctttata
     8101 caggaaaact agaccctttg ccaactgccc aaggccgttt tatttcagta acttccctga
     8161 gagtcaatcc cgcacctgct ccacgcatct agtccaaaca ggtggcgagg ccgcgcttga
     8221 tggcctcctc gggaagattg cgcccgatga acacaatctt cgagaagggc ttgcggtctc
     8281 cccacgtatc aagcacgtcg ctcacggcaa gcatgtgcac gccctggaag acgactcggc
     8341 ggtcggtgcc gctcagatag agaacgccct tgtagcgaag catgtcctga ccgtagacgc
     8401 tcgtgagcga agcgatgtag cgttcgagct tcatcggatt gaacggtcga tcggactcat
     8461 acacaaacga gtggatgtcg tcgttgtgat ggtgcatgtg atgatcgttg ttgaagaacg
     8521 caggatcaac atcaagcacg tcgttcatgt tgaagcccgt gatgttaagc acttcgtcaa
     8581 cagggcattc gcccatgttg acgcggcgga tcggggcgcg ggcgttcatc tgacgaagac
     8641 gctgctccat ggccgcaact tcctcggccg tgcagagatc cgtcttcgaa agcaggattc
     8701 ggtctgcaaa gccgacctga tcgcgcgcag cggcctcctc gtcaagcgtg gcggaaccgt
     8761 gcttgcagtc gacgaccgtc accacgccgt caagacggaa gaaggcggcg acggcatcgt
     8821 ccatgaagaa ggtctgggcg acggggccag gattggcaac gccgctagtc tcgatgacga
     8881 cgtagtcgaa cttgatctcg cccctgctgc gacgcaggcg cagatcagtg agaaccttgg
     8941 acaggtcgcc gcggatcgtg cagcagacgc agccgttgtt catgttcaca atctgttccc
     9001 tgtccgtctg aacaagcaga tccgtgtcga tgttctcctt gccgaattca ttttcgacaa
     9061 tggcgatctt gtagttgtgg ttctcgctga ggatgcgatt cagcagcgtc gtcttgccgg
     9121 caccgagaaa acccgtaaga atcgtcaccg gaatgtccgg ctgttgttct tcaaccatca
     9181 tttttttatc ctcgtcaata acgaaccaac cacgggcgag cccgtggtta tgcatgcaat
     9241 cagtctacgc ctcaaggcct caccaggaga tgaagtccct tcaggtattc
//
LOCUS       DBMDTI010000057         9254 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2083_length_9254_cov_2.637168, whole genome shotgun sequence.
ACCESSION   DBMDTI010000057 DBMDTI010000000
VERSION     DBMDTI010000057.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2083_length_9254_cov_2.637168"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="ACZPB6_04295"
     CDS             complement(<1..316)
                     /locus_tag="ACZPB6_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183726.1"
                     /translation="MAPVGRTTIPARGRRKTGVWKGHPLTAAFARCLIMSVTHRVTAP
                     LFDTASDFAFGNNLQTFIYDTFLEPTVPQAKRKGYEKADEKKGRRLREPSPLQRKAPL
                     QRQ"
     gene            368..1558
                     /locus_tag="ACZPB6_04300"
     CDS             368..1558
                     /locus_tag="ACZPB6_04300"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR1183727.1"
                     /translation="MQRLTHLYERSVFGGAELTRNDSAVLKALAILLIAAHNFFHQVK
                     PFPGENEMAFGEATFRNTVEQIAANPLDAFHPLVSFFGHYGVHVFILLSGYGLMKKAL
                     GIASRQGGISTADLFRMAGNQIAKIMLLTVVGVSVLILYKLMAYGGLPDAEFFRKYLV
                     FLTFTENLRPSDFGYFVTVWWFMALIVQCYLLFPFVYRLATGSAWGAAGVALLSMTGA
                     YAFYQPLLDAGVLVYATPLGQLPLFLLGAWLAAGRRIPATVVFGLAAVLPLTFVDPEF
                     FHLSFLSVVTVAFLLYGIFREKLSASRFLVWTGGLSMFIYIVHGDLRWLIIPIVNETQ
                     NVWFAYVAFFGFMTEVFLFALLARWISEKTRLFRLVPFRMKGGSQTQEAPSALPVRPA
                     LQGD"
     gene            complement(1642..4242)
                     /locus_tag="ACZPB6_04305"
     CDS             complement(1642..4242)
                     /locus_tag="ACZPB6_04305"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIR1183728.1"
                     /translation="MTAPEPDAAGAPAPTIREVFDRERERLAREWEAKHDSEAYLRAH
                     TSVLDTAVLAMLAESGLPADRIAVAAVGGYGRGELYPYSDIDLLVLTDESVKEDESCQ
                     TALATFTTRLWELGLTVGASVRSTSEFLSEAAEDVSIATTYLESRCLWGNGRLYAQAR
                     MHFAQTLDPRRFFRDKMLELARRHQKFEDTPYSLEPNIKESPGGLRDLQVFLWCAQAA
                     GIAQSVSDMASAGLITEREMHTLVACEHHLESLRITLHLLTHRPENRLLFDVQEALAS
                     ALGYSATAQLRPSEALMKRYYLNAKAVVQMSVIQLQAIADRLFGSYDNVPAVRLEAAF
                     VARGDEMDIAGDDVFRTDPNAMLRLFYVYHSHPELTRLSTRLLRALWHAAPTIDDAFR
                     HDLINQATFLDILRMPKGTYHSLKLMNTWGVLGRFLPPFRHIVGQMQHDLYHIFTVDQ
                     HTLRTVRNIRRFARSEFGHEYPLCSQIMGEIPDNWRLALAGLYHDIGKGLGGHHEIIG
                     AEKVTAFCRAFGLDDSTTDFIAFLVREHLTMSLTAQKRDISDPEVVRRFADVVKTKTR
                     LDALYLLTVADIRATSPKVWSPWKAQLLETLYHSTLAMLQGEDTAVSTEHTQAERKTR
                     ALKILGDTVPDQVREAFWRELNVVYFMRHTPEEIAWHTASLAERMHTTEPVVEVRRSE
                     RMGGILILLYLPDRKDLFLRAVAAIGKCSLSVVDARVHTTRHGWALDTFLVTDRYERE
                     DEEALSRKITREMMKLLTGDGPLPPAPKGKLSRRSRHFPTRTVVTTLPDESGRAWILN
                     IICTDRPGLLYSISEVLAQYRINLQTARIATLGERAEDVFLIDGPVLSDDETLLEVEA
                     KLVEAVAPPK"
     gene            complement(4223..5053)
                     /locus_tag="ACZPB6_04310"
     CDS             complement(4223..5053)
                     /locus_tag="ACZPB6_04310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1183729.1"
                     /translation="MPAEPAAYRPVIVIPVYNHPATVEDVVRRLLSTGTPVLLVDDAS
                     DPPAQEACERCRAMGAGLVRRQSTGGRGASILTGFKAARQAGFTHALVFDAGGRHDPA
                     AVGSFLELSRKHPNDLVCGYARTSSAYPARARLARRMADAGACLAALSLAPKDVMCRL
                     RVYPLAAVSQLSACIGLGRRAEFEFESLVRLMWMGLRLRELPVDPRPPADGVSHYQPV
                     RDTLRIAGMSAKLFIIMLTRLPAVLISRSTGWSPAPGLGSEHPQAHPSRSPSDDRSRT
                     "
     gene            complement(5147..5971)
                     /gene="map"
                     /locus_tag="ACZPB6_04315"
     CDS             complement(5147..5971)
                     /gene="map"
                     /locus_tag="ACZPB6_04315"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR1183730.1"
                     /translation="MAIHIKTPEEIAGLRIAGRLASELLDYLTPFVKPGITTGELDKL
                     AYDYTVNVQKCIPACLGYQPEGMTPYPATTCISINHVVCHGIPGEKKLKHGDIVNIDV
                     TSIKDGFHGDTSRMFVVGKPTIAGKRLCDLAFKTMWAGIEAVRPGGTFNDIGVACQAF
                     ADKNGISVVRDYGGHGIGRGFHEEPHVHHFPINHPTPKFVPGMVFTIEPMVNAGRYGV
                     TMLGDGWTVVTRDRSLSAQWEHMVLVTETGYDILTCSAGTPEPPDFVKGWKKPEGL"
     gene            6352..7110
                     /gene="rpsB"
                     /locus_tag="ACZPB6_04320"
     CDS             6352..7110
                     /gene="rpsB"
                     /locus_tag="ACZPB6_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR1183731.1"
                     /translation="MVSMREMLEAGCHFGHQTRFWNPKMGQYIFGARNKIHIINLEKT
                     VAKFEEACKFARQLSAQGGKILFVDAKRGGREIIAAEAQRCGSCWVDQRWLGGTLTNF
                     KTVRGTIKRLKDMEQQLEDGSAANLTKKEALDFQRNVEKLNKSIGGIKEMSALPDALF
                     IVDVGYHKIAIQEANKLGIPVIGIVDTNHSPDGVDYVVPGNDDSSKAVAIYARGIADA
                     VLAGRADSQTEIVEAAADEEFVEVAAEAEEQPAA"
     gene            7333..8217
                     /gene="tsf"
                     /locus_tag="ACZPB6_04325"
     CDS             7333..8217
                     /gene="tsf"
                     /locus_tag="ACZPB6_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR1183732.1"
                     /translation="MAAITAAMVKELRVKTDAPMMECKKALTEADGDMAKAEEILRVK
                     LGNKATKAAARIAAEGVVAVYLSDDRKLGAILEVNSETDFVAKNPEFLQMAADAVRAV
                     AEQNPADIEALAACKIGDATLEEVRKGLVGKIGENMTFRRFTRVEAKGELNSYIHGSK
                     IGVIVDVIGGNAELSHDVAMHIAAAKPKALDETGVDQAQIEAERRIAIEKAREAGKPE
                     AMLERIAEGTVKKFLKEVTLVNQPFVKDDKKSVGELVKAAGAQIGGYTLFVVGEGIEK
                     RVDDFAAEVAAQVAAAKA"
     gene            8517..9254
                     /gene="pyrH"
                     /locus_tag="ACZPB6_04330"
     CDS             8517..9254
                     /gene="pyrH"
                     /locus_tag="ACZPB6_04330"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR1183733.1"
                     /translation="MDQTAETAAKPAYKRILLKLSGEALMGEDSFGINRTVLARMVEE
                     IKSVVELGVQVGIVVGGGNIFRGVALGATGMDRATGDYMGMLATVMNAMALQDACRNN
                     GVEARVQSALNIEQVAEPYIRGKALRHLRLGRVVIFAAGTGNPFMTTDTTAALRGAEI
                     GAEIVIKATKVDGIYTADPKKDPTATMFDEITFDDALRSNLKVMDATAFALCREQGLP
                     IKVFNINKKGALRRVILGEQEGTLVHS"
BASE COUNT         1657 a         2779 c         3006 g         1812 t
ORIGIN      
        1 cctgacgttg caaaggcgcc tttcgctgca ggggagaagg ttctcggagc ctcctcccct
       61 ttttttcgtc cgccttttcg tatccttttc tcttcgcctg cgggacagtg ggttcgagga
      121 aggtgtcgta aataaatgtt tggaggttgt tgccaaacgc aaagtccgat gcagtgtcaa
      181 aaaggggagc cgtgacgcgg tgtgtcaccg acataatgag gcatcgggcg aaggctgcgg
      241 tgagcggatg tcccttccag acgccggtct tccgacgtcc tcttgccgga atcgtcgtcc
      301 gtccgaccgg agccatgctc cggcccctca acgcccggct tcgtgccgga ctcaccggat
      361 acgcgacatg caaaggctca ctcatctcta cgagcgatcg gtctttggcg gcgcggagct
      421 taccagaaac gactccgccg tcctcaaggc actggccatc ctcctcatcg ccgcgcacaa
      481 cttcttccat caggtcaagc ccttccctgg cgaaaacgag atggcctttg gagaggcgac
      541 cttccgaaac acggtcgagc agattgccgc caatcccctt gacgccttcc acccgctcgt
      601 ctccttcttc gggcactacg gcgtgcacgt cttcatcctt ctttccggct acggcctcat
      661 gaagaaggcg ctcggcatcg cctcaaggca gggcggtatt tcgacggccg atctcttccg
      721 catggcgggc aaccagattg ccaagatcat gcttctcacg gtcgttggcg tctccgttct
      781 catcctctac aagctgatgg cctacggcgg cctgcccgac gcggagttct tcaggaagta
      841 cctcgtcttc ctcaccttca ccgaaaatct ccgcccaagc gatttcggct acttcgtgac
      901 cgtctggtgg ttcatggcgc tcatcgtgca gtgttatctg ctcttcccgt tcgtctacag
      961 gcttgccacg ggcagcgcct ggggcgctgc gggcgtcgcg ctcctttcga tgacgggcgc
     1021 ctacgccttc taccagccgc ttctggacgc aggcgtcctc gtctatgcaa cgcctctggg
     1081 ccagctgccg ctcttccttc tcggcgcatg gcttgccgca ggccgccgca ttcccgcgac
     1141 cgtcgtcttc gggcttgccg ccgtgctgcc gctcactttc gtcgatcccg agttcttcca
     1201 cctgagcttc ctctcggtcg tgacggttgc cttccttctt tacggcatct tccgggagaa
     1261 gctctcggcc tcgaggtttc tcgtctggac gggcggcctc tcgatgttca tctacatcgt
     1321 tcacggcgat ctgcgatggc tcatcattcc gatcgtcaac gagacgcaga acgtctggtt
     1381 cgcctatgtt gccttcttcg gcttcatgac ggaagtcttc ctcttcgcgc tcctcgcacg
     1441 ttggatttcg gaaaagacgc gcctcttccg tcttgtgccc ttcagaatga agggtggaag
     1501 tcagactcag gaagcgccct ccgcacttcc tgtccgtccc gccttgcagg gagattgagt
     1561 ctctcctcag gcagcaaagg caaagccgcc cggcagcgtg tgactgcggg cggctttttc
     1621 atggcgcgag tgcttcttct tttatttcgg cggtgccact gcctcaacga gcttcgcttc
     1681 gacctcaagg agcgtctcgt cgtcggagag cacggggccg tcgatgagga agacgtcttc
     1741 ggctctctcg ccgagcgtgg cgatgcgggc cgtctgcagg ttgatgcggt attgggccag
     1801 cacttcggag atgctgtaga gcaggcccgg cctgtccgtg cagatgatgt tgaggatcca
     1861 ggcccggccg ctttcgtcgg gcagcgtcgt cacgaccgtg cgggtcggga agtggcggga
     1921 gcgtctggag agcttgccct tcggggcggg cggaagaggg ccgtcgcccg tgaggagctt
     1981 catcatctcc cgggtgatct ttctggagag cgcctcctcg tcctcccgct cgtagcggtc
     2041 ggtgacgagg aaggtgtcca gcgcccagcc gtggcgggtc gtgtgcacgc gcgcatcgac
     2101 gaccgaaagg ctgcacttgc cgatcgccgc tacggctctc aggaagaggt ccttgcggtc
     2161 ggggaggtag agaaggatta ggatgccgcc catgcgctcg cttcttctga cttcgacgac
     2221 gggttctgtc gtgtgcatgc gctcggcgag cgatgcagtg tgccaggcga tttcctcggg
     2281 cgtgtggcgc atgaagtaga cgacgttgag ctcgcgccag aaggcctctc ggacctgatc
     2341 aggaaccgtg tcgccgagaa ttttgagcgc tcgggtcttg cgttcggcct gcgtgtgttc
     2401 ggtcgaaacg gccgtgtcct cgccctgcag catggcgagg gtcgagtgat agagcgtttc
     2461 gagaagctgg gccttccagg gcgaccagac cttggggctc gtcgcacgga tgtcggcgac
     2521 ggtgagaagg taaagggcgt cgagtcgcgt cttggtcttg acgacgtccg cgaatctgcg
     2581 cacgacctcg gggtcggaga tgtcgcgctt ctgggccgta aggctcatcg tgaggtgctc
     2641 cctcacgagg aaggcgatga agtctgtcgt tgaatcgtcg agcccgaagg cgcggcagaa
     2701 ggccgtgacc ttttcggcgc cgatgatctc atggtggccg ccgagtccct ttccgatgtc
     2761 atggtagagg cctgcgagcg ccagacgcca gttgtcggga atctcgccca tgatctggga
     2821 gcagagagga tactcgtgcc cgaattcact gcgcgcgaag cggcggatgt tgcgcactgt
     2881 cctgagcgtg tgctggtcga ccgtgaagat gtggtagagg tcgtgctgca tctgtcccac
     2941 gatgtgcctg aagggcggca ggaagcgtcc gagcacgccc cacgtgttca tgagcttgag
     3001 cgagtggtag gttcccttgg gcatcctgag gatgtcgagg aaggtcgcct gattgatcag
     3061 atcgtgccgg aaggcgtcgt cgatcgtcgg ggcggcgtgc cagagtgcgc gcagcaggcg
     3121 cgttgagagg cgagtgagct cgggatgcga atggtagacg tagaagagcc tcagcatcgc
     3181 gttgggatcg gtcctgaaga cgtcgtctcc ggcgatgtcc atctcgtctc cgcgagcgac
     3241 gaacgcggcc tcgagcctga cggcgggcac gttgtcgtag ctgccgaaca gacggtcggc
     3301 gattgcctga agctgaatga ccgacatctg cacgacggcc ttggcattga ggtagtagcg
     3361 cttcatcaaa gcctcggagg gcctcagctg tgccgtggcg ctgtagccga gtgcgctcgc
     3421 aagcgcctcc tgcacgtcga agagcagacg gttttccgga cggtgtgtga gcagatgcag
     3481 cgtgatgcga agcgactcga gatggtgttc gcaggcgacg agcgtatgca tctcgcgctc
     3541 ggtgatgagg ccggcggagg ccatgtcgga gacggactgt gcaatgcctg cggcctgggc
     3601 gcaccagaga aagacctgca ggtcgcgaag gcctccgggg ctctccttga tgttgggctc
     3661 gagcgaatag ggcgtgtcct cgaacttctg atggcgtcgg gcaagttcaa gcatcttgtc
     3721 gcggaagaag cgcctggggt caagcgtctg cgcgaagtgc attctcgcct gggcgtagag
     3781 tctgccgttg ccccagaggc agcggctttc gagatacgtg gtcgctattg agacgtcctc
     3841 ggcggcctcg gagagaaatt cagaggtact gcgcaccgat gcgccgaccg tgagaccgag
     3901 ctcccagagt ctggtcgtga aggtggcgag ggccgtctga caggactcgt cctccttgac
     3961 ggactcgtcc gtgaggacga gaaggtcgat gtcggaatag ggatagagct cgccgcggcc
     4021 gtagccgccc acggccgcca ccgcaatgcg gtcggcgggc aggccgctct cggcgagcat
     4081 ggcgagaacc gccgtgtcga gcacgctcgt gtgggctctt agataggcct cgctgtcgtg
     4141 cttggcctcc cattcgcgag cgagccgctc gcgttcgcgg tcaaagacct cgcggatggt
     4201 gggggcgggt gcgcctgcgg cgtcaggttc gggagcggtc atcggatgga ctcctggacg
     4261 gatgtgcttg agggtgctcg gagccgagtc ccggggcggg cgaccagccc gtggaacggg
     4321 agatcagcac ggcggggagc ctcgtgagca tgatgatgaa gagcttggcg ctcatgcctg
     4381 cgatgcggag cgtgtcgcgc acgggctggt agtgggagac gccgtcggcg ggcggtctgg
     4441 gatcgacggg aagctcgcgc aggcgaagtc ccatccacat gaggcgcacg agtgactcga
     4501 attcgaattc ggcgcgtctg ccgagtccga tgcaggcgga gagctgcgag acggcggcga
     4561 gcgggtagac gcgcaggcgg cacatgacgt ccttgggcgc aagactgagt gcggccagac
     4621 atgcacctgc gtcggccatg cggcgtgcga gacgtgcgcg ggcgggatag gcgctcgacg
     4681 tgcgggcata gccgcagacg aggtcgttgg gatgcttgcg ggagagttcg agaaagctgc
     4741 cgacggcggc gggatcatgc ctgccgcctg cgtcgaagac gagcgcgtgc gtgaagcccg
     4801 cctgccttgc ggccttgaag cccgtgagga tggaggcgcc cctgccgccc gtcgactgac
     4861 ggcggacgag gccggcgccc atggccctgc atcgttcgca ggcttcctgc gcgggcgggt
     4921 cggaggcgtc gtcgacgaga aggacgggag tgcccgtgga gagcaggcgt ctgacgacgt
     4981 cctccaccgt ggcgggatgg ttgtagaccg ggattacgat gaccggcctg tatgcggcgg
     5041 gttctgcggg catgcggaaa ctcccacggg ggcggggctc ctgcgcggac tgggccgttc
     5101 aggatgcggt gcaggccgtc ggagacggcc tgcggaggtt tcaggctcag aggccctcgg
     5161 gcttcttcca gcccttgacg aagtcgggcg gttcgggtgt gcccgcggag cacgtcagga
     5221 tgtcgtagcc cgtttcggtg acgaggacca tgtgctccca ctgtgcggag agcgagcggt
     5281 cgcgcgtgac gacggtccag ccgtcgccga gcatcgtgac gccgtagcgg ccggcgttca
     5341 ccatgggttc gatcgtgaag accatgcccg ggacgaactt cggcgtcggg tggttgatcg
     5401 ggaagtggtg gacgtggggc tcctcgtgga agccgcggcc gatgccgtgg ccgccgtagt
     5461 cgcgcacgac ggagatgccg ttcttgtcgg cgaaggcctg acaggcgacg ccgatatcgt
     5521 tgaacgtgcc gccaggacgc acggcctcga tgcccgccca catcgtcttg aaggcgaggt
     5581 cgcagaggcg cttgcccgcg atggtcggct tgccgacgac gaacatgcgg gacgtgtcgc
     5641 cgtggaagcc gtccttgatg ctcgtgacgt cgatgttgac gatgtcgccg tgcttgagct
     5701 tcttttcacc cggaatgccg tggcagacca cgtggttgat cgagatgcag gtggtggcgg
     5761 gatacggcgt catgccttcg ggctggtagc cgaggcaggc ggggatgcac ttctggacgt
     5821 tgaccgtgta gtcgtaggcg agcttgtcga gttcgcccgt ggtgatgccc ggcttgacga
     5881 agggcgtcag gtagtcgaga agttcggagg cgaggcggcc ggcgatgcgc aggcccgcaa
     5941 tctcctccgg cgttttgatg tggatggcca tgatgtgcgg aatttgttgc tgtttaatag
     6001 tgctgcatga ccgcaggcgg tccctgctgt cggggcggcg ggcggtcctt atcggcgata
     6061 gtacaccaat tcgacggggg caactgcagg cggacgcccc gagggcggac gggaggcgcg
     6121 tgatggaccg aagaaaattg cccgcgacgc ttgcaacgcc gttcaaccct cacgtataat
     6181 cgtgcgcttt cagatccgga gcctcgtgcc ccggagtttt tcatcctgcc cggtgaacct
     6241 tggtcggtcc cgggtgaaat cagcgcacgt tctgttccga ggtgctgtgc aggcttgtcc
     6301 tgcgctcaac ttatcaggac gtgtcagact ctaaaccttg gagaattaat catggtcagc
     6361 atgcgtgaaa tgcttgaagc tggttgccat tttggtcacc agacccgttt ctggaacccg
     6421 aagatgggtc agtacatctt tggcgcccgc aacaagattc acatcatcaa cctcgaaaag
     6481 acggttgcca agttcgaaga agcctgcaag ttcgcccgcc agctctccgc tcagggcggc
     6541 aagatcctct tcgtcgacgc caagcgcggc ggtcgcgaaa tcatcgccgc cgaagcccag
     6601 cgttgcggca gctgctgggt tgaccagcgc tggctcggcg gtacgctcac caacttcaag
     6661 acggtgcgcg gcacgatcaa gcgtctcaag gacatggagc agcagctcga agacggctct
     6721 gctgccaacc tgacgaagaa ggaagccctc gacttccagc gcaacgttga aaagctcaac
     6781 aagtccatcg gtggcatcaa ggaaatgtcc gctcttccgg acgctctctt catcgttgac
     6841 gtcggctacc acaagatcgc catccaggaa gccaacaagc ttggcatccc ggtcatcggc
     6901 atcgtcgaca cgaaccactc tccggatggc gttgactacg ttgttccggg caatgacgac
     6961 tcctccaagg ctgtcgccat ctacgcccgc ggcatcgctg acgccgttct ggccggccgt
     7021 gccgacagcc agacggaaat cgttgaagcc gccgctgacg aagagttcgt cgaagtggct
     7081 gctgaagccg aagaacagcc cgctgcctaa tttcggttga agcggattcc ctgcaccggc
     7141 ggtcgacata cattacaatg tcgaccgttc gcagagaacc gcgactctgt ccttaagcag
     7201 gagcctctgg caggccgaat tacttctgcc cctcaggtct ccggctgaag aaagggggcg
     7261 acatcatgtc accccctttt tttcgtgatt ctgaattcta aaattttcta ctgatactct
     7321 aggagagtga acatggctgc tattaccgcc gcgatggtca aggaactgcg cgtcaagacg
     7381 gacgccccga tgatggaatg caagaaggcc ctcacggaag ctgacggcga catggccaag
     7441 gctgaggaaa tccttcgcgt caagctcggc aacaaggcca cgaaggccgc cgcccgcatc
     7501 gctgccgaag gcgtcgtcgc tgtctacctt tccgacgatc gcaagctcgg cgccattctc
     7561 gaagtcaact ccgaaacgga cttcgttgcc aagaacccgg aattcctgca gatggctgct
     7621 gacgccgtcc gcgccgttgc cgaacagaac ccggccgaca tcgaagccct cgccgcctgc
     7681 aagatcggcg atgccacgct tgaagaagtc cgcaagggcc tcgttggcaa gatcggcgaa
     7741 aacatgacgt tccgccgctt cacgcgcgtt gaagccaagg gcgagctcaa ctcctacatc
     7801 cacggttcca agatcggcgt catcgtcgac gtgatcggcg gcaatgccga actctcccac
     7861 gacgtggcca tgcacatcgc cgccgccaag ccgaaggctc tcgacgagac cggcgttgac
     7921 caggcccaga tcgaagccga acgccgcatc gccatcgaaa aggcccgcga agccggcaag
     7981 ccggaagcca tgctcgaacg catcgccgaa ggtacggtca agaagttcct caaggaagtc
     8041 acgctcgtca accagccgtt cgtcaaggac gacaagaagt ccgtcggcga actcgtgaag
     8101 gctgccggcg cccagatcgg cggctacacg ctcttcgtcg ttggtgaagg catcgaaaag
     8161 cgcgttgacg acttcgctgc tgaagtcgcc gcccaggttg ccgccgccaa ggcctaatct
     8221 gacgcgtgca cgaatcagcc caagcggctg attcagcctc aagtgaaaac gggatgccct
     8281 gttgcgggac atcccgtttt ttcatggtgg ccaagcaagc acaaaccgtg ccgaaacggc
     8341 tcgtgggcag tgtcccgcag gctttgtctg agtctcgtca gggcctctga aaatattgtt
     8401 tgcgtaaaaa tcgggctagc ttataattag gtaattttgc ctacgggacg tacctttcga
     8461 cgccctgcag gcatggttga cagcacaaat tacagtttga ctttcggagt gacgacatgg
     8521 atcagacggc ggaaacggcg gccaagcccg cctacaagcg cattcttctc aaactttccg
     8581 gcgaggccct gatgggcgaa gacagcttcg gcatcaaccg tacggttctt gcccgcatgg
     8641 ttgaggaaat caagagcgtc gtcgagctcg gcgttcaggt cggcatcgtc gtgggcggtg
     8701 gcaacatctt ccgcggcgtc gcactcggcg ccaccggcat ggaccgcgcc acgggcgact
     8761 acatgggcat gctcgccacg gtcatgaacg ccatggccct tcaggacgcc tgccgcaaca
     8821 acggcgtcga ggctcgcgtt cagtccgccc tcaacatcga gcaggtcgcc gagccgtaca
     8881 tccgcggcaa ggccctccgt catcttcgtc tcggccgcgt ggtgatcttc gccgccggca
     8941 cgggcaaccc cttcatgacg accgacacga cggccgccct gcgcggcgcc gaaatcggcg
     9001 ccgagatcgt gatcaaggcc acgaaggtcg acggcatcta cacggccgac ccgaagaagg
     9061 atccgaccgc cacgatgttc gacgaaatca ccttcgacga tgcgctccgc tccaacctca
     9121 aggtgatgga cgccaccgcc ttcgcgctct gccgcgaaca gggcttgccg atcaaggtct
     9181 tcaacatcaa caagaagggc gctctgcgcc gcgtcattct cggcgaacag gaaggcacgc
     9241 tcgtgcattc ctga
//
LOCUS       DBMDTI010000058         9238 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2086_length_9238_cov_3.639378, whole genome shotgun sequence.
ACCESSION   DBMDTI010000058 DBMDTI010000000
VERSION     DBMDTI010000058.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2086_length_9238_cov_3.639378"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="ACZPB6_04335"
     CDS             <1..558
                     /locus_tag="ACZPB6_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR1183734.1"
                     /translation="TAYRKMAEHAMLVATLRHLIPPRLCVTKDLPIFCDPNWDHAALE
                     IEAEAGDAVDEKVVQFALYARDHEFARTPEDVLFRRLRLGQMNEARTNALLPKIAAAF
                     GLPVEECCCADEACTCEEGKCECEEDKCECEKAECECKAEVCEAEVCECKAEEKAEAA
                     PVEAEKVIDVEVVEEKPAEEAEQKK"
     gene            968..1396
                     /gene="rplM"
                     /locus_tag="ACZPB6_04340"
     CDS             968..1396
                     /gene="rplM"
                     /locus_tag="ACZPB6_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR1183735.1"
                     /translation="MKTFSAKPLEVKRDWFVVDASDKVLGRLASEIALRLRGKHKPEF
                     TPHVDTGDFIVVINADKLKLSAEKDTKKTYYRHTGYPGGIYETTFREMQDKHPGRALE
                     IAVKGMLPKGPLGYAMIKKLKIYAGAEHPHSAQQPKVLDL"
     gene            1416..1808
                     /gene="rpsI"
                     /locus_tag="ACZPB6_04345"
     CDS             1416..1808
                     /gene="rpsI"
                     /locus_tag="ACZPB6_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR1183736.1"
                     /translation="MIGNYNYGTGRRKSSVARVFIKRGTGKIVINGRPLEQYFHRETG
                     RMIVMQPLQLTENTETFDIMVNVCGGGESGQAGAIRHGITRALVDYDAGLKSVLSNAG
                     LVTRDAREVERKKVGLRKARRAKQFSKR"
     gene            2071..2448
                     /gene="erpA"
                     /locus_tag="ACZPB6_04350"
     CDS             2071..2448
                     /gene="erpA"
                     /locus_tag="ACZPB6_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR1183737.1"
                     /translation="MTEKIETPQEEMPELMPDPINFTDAAVAKVKELMDEEGNPSLKL
                     RVFVQGGGCAGFQYGFTFDEVQNPDDAVMEKGGVTLLIDSMSYQYLVGAKIDFKDDLS
                     GAQFVIDNPNAVTTCGCGSSFGV"
     gene            complement(2543..3646)
                     /locus_tag="ACZPB6_04355"
     CDS             complement(2543..3646)
                     /locus_tag="ACZPB6_04355"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR1183738.1"
                     /translation="MTERITIGLMSGTSLDGTDAVAMAFDGRVMRSLGHSSLAFPPNL
                     RAALAALTLPGPDEIERMGEASVALARHYAEATGLLLARTGLSPADVAALGVHGQTIR
                     HRPQKGFTLQLNHPALVAELTGIDVVADLRSRDVAAGGEGAPLVPAFHAECFTGDVPR
                     VVLNIGGIANISVLPARSESPTILGGDTGPGNMLIDTWTQRITGALYDRDGRWAASGR
                     VSTTLLDDLLSEPYFALPIPKSTGRELFSPAWLDARLCGHESLPPEDVARTLVELTAR
                     TITNTVARFAPDAAELYVCGGGVYNKTLMQAIASHFPGLTATTEALGVPPMEVEAAAF
                     AWLAERFLASLPGNLPAVTRAAGPRILGALYPR"
     gene            complement(3727..5109)
                     /locus_tag="ACZPB6_04360"
     CDS             complement(3727..5109)
                     /locus_tag="ACZPB6_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR1183739.1"
                     /translation="MVVIGTTGAMLGVSPSLAQEKHRADFVTGSVDDSVLTQALDALR
                     RNGSVTPRSLTVRNGETLSSLLARLEVVDPQAVSYIRSQRMLRPLVMPQDGQYVNAGV
                     LPDGRLAFLHLYLEGPHPQDSRRIEVTRTGELFAAAEYPVAFDTLEESISGVASSGFA
                     ATVESLRIPADIAEQLKVVWEGADNPVRTLKQGDSLRLVFERKFAGGDFIRNGQLLAV
                     QVVSGEDVHEAYWFENDSRAGGFYTLAGRASQQTFMRVPLDVKDVSSEFAPLRRHPVT
                     GVVRPHNGTDFRAPSGSRIFAAADGVVTFVGYERKGYGRYVKIDHGLGRTTLYAHMSR
                     ITKNLRAGQKVERGDVIGYVGRTGLATGPHLHYELMFDGVQINPRTADLPDTENLTAF
                     QVAQLKTIARPIQARFDLLAESESLPVPTVLRTHRDDATAVAKHLLGDEEQAEGASRV
                     AHRVTSMKED"
     gene            5475..6734
                     /gene="tyrS"
                     /locus_tag="ACZPB6_04365"
     CDS             5475..6734
                     /gene="tyrS"
                     /locus_tag="ACZPB6_04365"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR1183740.1"
                     /translation="MTVELEKAQEAKKLPVTDDIREAMALIRRGTDTFLIEEEFEQKL
                     ARSKMTGEPLRCKLGLDPTAPDIHIGHTVVLNKLRQLQDLGHTVIFLVGDFTAAIGDP
                     SGRNATRPPLSHEQIVQNAQTYLNQAGLVLDLDRTEVRYNSEWCNKLGSVGLIQLASR
                     YTLARLLERDDFAKRYAEQAPIAMHELIYPLMQGYDSVALKADLELGGSDQRFNLLVG
                     RELQRQYGQEPQCILTMPLLVGLDGQVKMSKSKHNYIGITDAPNDMFGKVMSISDSLM
                     WDWYDLLSLRGNAEIAQLKKECSEGRNPRDAKVLLAKEIVGRFHSDCAANAAEDEFNA
                     RFRAGAMPSDISEVTVEAPAGEIGVGQMLKAAGLVSSTSDANRNIDQGGVRIEGEKVS
                     DKGLKLKAGTVVTVQVGKRKWARITIA"
     gene            6745..7206
                     /gene="dtd"
                     /locus_tag="ACZPB6_04370"
     CDS             6745..7206
                     /gene="dtd"
                     /locus_tag="ACZPB6_04370"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR1183741.1"
                     /translation="MIVLIQRVAEASVSVEGRVTGAVGRGLLALVCGEPGDTPAVIGK
                     LARKTARLRIFEDEAGRMNRSVVDVGGDVLCVSQFTLAADTMSGNRPSFSRAAPPEEG
                     LAAFNAFVTALRGEGLSCPTGEFGAHMRVSLVNDGPATFSLHLRADGAGAD"
     gene            7303..7905
                     /locus_tag="ACZPB6_04375"
     CDS             7303..7905
                     /locus_tag="ACZPB6_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR1183742.1"
                     /translation="MKITSDNFKQGDFIPEALAWGRIGEDGSTVRSTNLNPQLSWTDA
                     PEGTASFVLACLDDDVPTDLAERDASGEIPVSQPRRRFVHWVQPNCPADVTSIAEGAL
                     AEERKTTPGFGAVCINDYSRGGTPAPGETGTGYDGPCPPFFDARWHYYRFMVFALDAP
                     RLELPENATWQDVDAAMKGHVLASAELVGRYTLNPRLAAL"
     gene            7983..>9238
                     /gene="glyA"
                     /locus_tag="ACZPB6_04380"
     CDS             7983..>9238
                     /gene="glyA"
                     /locus_tag="ACZPB6_04380"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR1183743.1"
                     /translation="MFKRSNTIEAVDPAVWDVISHETARQEDHIELIASENYASPAVM
                     QAQGSCLTNKYAEGYPGKRYYGGCEHVDVVEQLAIDRAKKLFCEPAGVEMAVNVQPHS
                     GAQANSAVYFGLLEVGDTIMGLSLAEGGHLTHGMHLNYSGRYFKVVPYGLDANEAIDY
                     DRVEQLARECKPKIIVAGASAYSLRIDFKRFAEIAHSVGAYLMVDMAHYAGLIAAGVY
                     PTPFGHADIVTTTTHKTLRGPRGGLIFCRPDLEKKINSAVFPGMQGGPLMHVIAAKAV
                     ALGEALDPSFKVYGEQVMKNAAAMAEELVKRGLRIVSGHTESHVMLVDLRPLKITGKA
                     AETLLNAAHITVNKNAIPNDPEKPFVTSGIRLGSPAMTTRGFGEAEARKVANLIVDLL
                     EAPEDQAVFERVKAEVANLTAAFPVYG"
BASE COUNT         1747 a         2944 c         2961 g         1586 t
ORIGIN      
        1 acggcctacc gcaagatggc cgagcacgcc atgctcgtcg ccacgctgcg tcacctcatc
       61 ccgccgcgtc tctgcgtcac gaaggatctg ccgatcttct gtgatcccaa ctgggaccat
      121 gccgctcttg aaatcgaggc cgaagccggt gacgccgtcg acgaaaaggt cgttcagttc
      181 gccctctacg cccgcgacca cgagttcgct cgcacgcccg aggacgtgct cttccgccgt
      241 ctgcgcctcg gccagatgaa cgaggctcgc acgaacgccc tgctgcccaa gatcgccgcc
      301 gccttcggcc tgcccgtcga ggaatgctgc tgcgccgacg aagcctgcac ctgtgaagaa
      361 ggcaagtgcg aatgcgagga agataagtgc gagtgcgaga aggccgaatg cgaatgcaag
      421 gccgaggtct gcgaagccga agtctgcgaa tgcaaggctg aggaaaaggc cgaggctgcg
      481 cccgttgaag ccgaaaaggt gatcgacgtc gaagtcgtcg aggaaaagcc ggccgaagag
      541 gctgagcaga agaagtaaga ccgatgcctg aagggccccc gggccctttg gccgccggct
      601 ttcggtgcgg gcgactccat ggcggaggcg cccgtgccgt ttcttttggc gtgccgcgct
      661 gtctcttgag gacggcggcg gacggaaata aaaaaatcgg tccttcttgt ggacaagtaa
      721 gggcgattag ggtaaaatcg cgatcctgac tttgcttcac ggattgctta ccccatcttt
      781 ttccactggc gccgtgattc acacgcgaag ttcagaacgc catatttcta gggcgaaccc
      841 aaattcaggt tcgcttttgt ttttattcag catctgcatg tcagcgcggg tcctcagaaa
      901 tgatgacgga ccgcggcgcc atccgttgag cgttgcagca gctgccttac ttattaggtg
      961 aaacgcaatg aagaccttct cggccaagcc gcttgaggtt aagcgcgact ggttcgtggt
     1021 cgatgccagt gacaaggtgc tcggccgtct tgccagcgaa atcgctctgc gccttcgtgg
     1081 caagcacaag cccgaattca cgccccatgt cgacacgggt gactttatcg tcgtgatcaa
     1141 cgcggacaag ctcaagcttt ccgctgaaaa ggacacgaag aagacgtact atcgccacac
     1201 gggctatccc ggcggcatct acgaaacgac gttccgcgaa atgcaggaca agcacccggg
     1261 ccgcgctctg gaaatcgccg tcaagggcat gcttccgaag ggcccgctcg gctacgccat
     1321 gatcaagaag ctcaagatct acgccggcgc cgaacacccg cactccgccc agcaacccaa
     1381 ggttcttgac ctctaatttc cgaaaggctt gaaagatgat cggtaactac aactacggca
     1441 ccggccgccg caagagctcg gtcgctcgtg ttttcatcaa gcgcggcact ggcaagatcg
     1501 tgatcaacgg ccgtccgctg gaacagtact tccaccgtga aacgggccgc atgatcgtca
     1561 tgcagccgct tcagctcacg gaaaacaccg aaacgttcga catcatggtc aacgtttgcg
     1621 gtggtggcga atccggccag gccggcgcca tccgtcacgg catcacgcgc gccctcgttg
     1681 actacgacgc cggcctcaag agcgttctct ccaacgccgg tctcgtgacc cgcgacgctc
     1741 gtgaagtcga acgtaagaag gtcggtctgc gcaaggcccg ccgcgccaag cagttctcca
     1801 agcgctaatc gcttcgagta cggatttgca gaaggctcgt ccttctgcgg atcccggcaa
     1861 aaagaaggtc gcctgcatca agcagcgcgg ccttttttgt cgtctgcgcg tgccgaatct
     1921 gacggcgaga cccgtcacga gcgcgctttt tcgtgtagca tagatgatcg cttgaacaac
     1981 gccgtgaacg gcctccgccc atcgtggaag agaagccccg gcgtcccgtc acatttttgg
     2041 agggccgtcc tttggacggc acataccccg atgactgaaa agattgaaac gcctcaggaa
     2101 gaaatgcccg agctcatgcc cgatcccatc aacttcaccg acgccgccgt cgcgaaggtc
     2161 aaggagctga tggacgaaga gggcaatccc agcctcaagc tgcgcgtgtt cgttcagggc
     2221 ggcggctgcg ccggcttcca gtacggcttt acgttcgacg aggtccagaa ccccgacgac
     2281 gccgtgatgg aaaagggcgg tgtgacgctt ctcatcgatt ccatgagcta ccagtacctc
     2341 gtcggcgcca agatcgactt caaggacgac ctgagcggcg ctcagttcgt catcgacaat
     2401 cccaatgcgg tgacgacctg cggctgcggc tcctccttcg gagtctgatg ctggaggacc
     2461 gaaggcccga cgacacaaaa acaaacggac gacgttccat gcgaacgccg tccgtttttt
     2521 catagacctg cttccgtcct gatcagcgcg gatagagggc gccgagaatt cgcggtcccg
     2581 ccgcccgcgt gaccgcaggc aggtttccgg ggaggcttgc gagaaagcgc tccgcaagcc
     2641 aggcgaaggc agccgcctcc acctccatgg gcggcacgcc cagagcttcg gtcgtggccg
     2701 tgaggccggg gaaatgcgag gcgatggctt gcatgagggt cttgttgtag acaccgccgc
     2761 cgcagacgta aagctcggcg gcgtcagggg cgaaccgtgc gacggtgttc gtgatcgtgc
     2821 gggcggtcag ttcgacgagc gttcgtgcga cgtcttcggg cggcaggctc tcatggccgc
     2881 aaaggcgggc gtcgagccag gcgggcgaga agagctccct gcccgtcgac ttcggaatgg
     2941 gaagtgcaaa gtagggctcg ctcagaaggt cgtcgagaag cgtcgtgctg acgcggcctg
     3001 aggccgccca ccggccgtcc cggtcgtaaa gggctcccgt gatgcgctgc gtccatgtgt
     3061 cgatgagcat gttgcccgga cccgtgtcgc cgccgaggat ggtgggggac tcgctgcgag
     3121 cgggcagaac ggagatgttg gcgatgccgc cgatgttgag gacgacgcgc gggacgtctc
     3181 ccgtgaagca ttcggcgtgg aatgcgggca cgagcggcgc tccctcgcct cctgcggcca
     3241 cgtcgcggga gcgcaggtcc gcaacgacgt cgatgcccgt gagctctgcg acgagcgcgg
     3301 gatggttgag ctgcagcgtg aagccctttt gaggacgatg gcggatcgtc tggccgtgca
     3361 cgccgagtgc ggcgacgtcg gcgggggaga gtcccgttcg ggccagaaga aggccggtgg
     3421 cttctgcata gtggcgagca agcgcgacgg aggcttcgcc catgcgctcg atttcgtcgg
     3481 ggccgggcag cgtgagcgcc gcgagcgcag cgcgcaggtt gggcggaaag gcgagcgagg
     3541 agtgcccgag actcctcatg acacggccgt cgaaggccat ggcgacggcg tccgtgccgt
     3601 caaggctggt gccggacatg agtccgatcg taatgcgttc ggtcatggtg gttccctgcg
     3661 gaaatggcgg acaaggaaaa gccgacgcca tggcgccggc tttctgatgc ctgcatggag
     3721 aaggaatcag tcttccttca tcgaggtgac gcggtgcgcc accctggaag cgccttcggc
     3781 ctgctcctcg tcgccgagaa gatgcttggc gacggcggtt gcgtcgtccc tgtgggtgcg
     3841 caggacggtc ggaacgggaa gcgactcgct ttcggcaagc aggtcgaagc gggcctgaat
     3901 cggacgggcg atcgtcttga gctgggccac ctggaaggcg gtcaggtttt cggtgtcggg
     3961 caggtcggcc gtgcgcggat tgatctgcac gccgtcgaac atcagttcgt agtgcagatg
     4021 cggaccggtg gcaaggcccg tgcggcccac gtagccgatc acgtcgccgc gctcgacctt
     4081 ctggcctgcg cgcaggttct tggtgatgcg ggacatgtgc gcgtagagcg tggtgcggcc
     4141 gaggccgtgg tcgatcttga cgtagcggcc gtagcccttg cgctcgtagc ccacgaaggt
     4201 gaccacgccg tcggcggcag cgaagatgcg ggagcccgag ggggcgcgga agtcggtgcc
     4261 gttgtgcggg cgcacgacgc ccgtgacggg atgacggcga agcggcgcga actcgctgct
     4321 cacgtccttg acgtcgagcg gtacacgcat gaaggtctgc tgcgaggcgc ggccagcgag
     4381 cgtgtagaag ccgccggcac ggctgtcgtt ctcaaaccag taggcctcgt ggacgtcctc
     4441 gcccgagacc acctgaacgg caagcagctg gccgttgcgg atgaagtcgc cgcctgcgaa
     4501 cttgcgctcg aagacgaggc ggagcgagtc gccctgcttg agggtgcgga cgggattgtc
     4561 ggcgccctcc cagacgacct tgagctgttc ggcgatgtcg gcgggaatgc gcagggactc
     4621 gacggtggca gcgaagcccg aggaggcgac gccggagatg ctttcctcga gcgtgtcgaa
     4681 ggcgacgggg tattcggcgg cggcgaagag ctcgcccgtg cgggtgacct cgatgcggcg
     4741 gctgtcctga gggtgcgggc cctcgagata gaggtgcagg aaggcaagtc ggccgtcggg
     4801 cagaacgcct gcgttgacgt actggccgtc ctgcggcatg acgagcggac gcagcatgcg
     4861 ctgcgagcgg atgtaggaga cggcctgagg gtcgacgact tcaaggcgtg cgagcaggga
     4921 ggagagggtc tcgccgttgc gtacggtcag gctgcggggc gtaacggagc cgttgcggcg
     4981 cagggcgtcg agcgcctgcg tgagaacgga gtcgtcaacg gagcccgtca cgaagtccgc
     5041 gcggtgcttt tcctgcgcga gactgggcga gacgccgagc atggcgcccg tcgttccgat
     5101 cacgaccatc gaggctatcc ccgccgttgc ggccccggta ctcagtctgc acgcgtggag
     5161 aagcggggac gaggcccaga ccttgagcca ggatcgaaac gccctcccgg cggtgcggag
     5221 ctgccgggca atgagggagg gagcggtcat gatgagtcgg agttgcgaaa ggcgcggaaa
     5281 aagggaacaa tacttagatt gccattttaa atcagaaccg ctgtgcgtcc cgcctaaaaa
     5341 accttaaaaa tggttacagg cgggcatttc atgcgtctcc ggggcctttt gcatgacttt
     5401 gtgtgcagat gagcctgcga acgggcgctg caacgcggcg gccccagttt gtttaagtca
     5461 ttgaagggat actgatgacc gttgaattag agaaggctca ggaggccaag aagctccctg
     5521 ttaccgacga catccgcgag gcgatggccc tcatccgccg cggcaccgac acgttcctca
     5581 ttgaggagga gttcgaacag aagctcgccc gctccaagat gaccggtgaa ccgctgcgct
     5641 gcaagctcgg cctcgacccg acggctcccg acatccacat cggccacacg gtcgtgctca
     5701 acaagctgcg ccagcttcag gacctcggcc acacggtgat cttcctcgtg ggcgacttca
     5761 cggccgccat cggcgatccg tccggccgca acgccacgcg tccgccgctc tcgcacgagc
     5821 agatcgtcca gaacgcccag acctacctca atcaggccgg cctcgtgctc gaccttgacc
     5881 gcaccgaagt ccgctacaac tccgaatggt gcaacaagct cggctccgtg ggcctcattc
     5941 agcttgcctc ccgctacacg ctcgcacgcc ttcttgagcg cgacgacttc gccaagcgct
     6001 acgccgagca ggctccgatc gccatgcacg agctcatcta cccgctgatg cagggctacg
     6061 actccgtggc cctcaaggcc gacctcgagc tcggcggctc cgaccagcgc ttcaacctgc
     6121 tcgtcggccg cgaactccag cgccagtacg gtcaggagcc ccagtgcatc ctgacgatgc
     6181 cgcttctcgt gggtcttgac ggtcaggtca agatgagcaa gagcaagcac aactacatcg
     6241 gcatcaccga cgctccgaac gacatgttcg gcaaggtgat gtccatctcc gactcgctga
     6301 tgtgggactg gtacgacctg ctgagcctgc gcggcaacgc cgagatcgcc cagctcaaga
     6361 aggagtgctc cgagggccgt aatccgcgcg acgccaaggt gctgctcgcc aaggagatcg
     6421 tgggccgctt ccattcggac tgcgccgcca atgccgccga agatgaattc aacgcccgct
     6481 tccgtgccgg cgccatgccg agcgacattt ccgaagtgac ggtcgaggct cccgccggcg
     6541 agatcggcgt cggccagatg ctcaaggccg ccggtctcgt ctcctccacg tccgacgcca
     6601 atcgcaacat cgatcagggc ggcgtgagga tcgaaggcga gaaggtctcc gacaaggggc
     6661 tcaagctcaa ggccggcacc gtcgtgacgg tgcaggtcgg caagcgcaag tgggcccgca
     6721 tcacgatcgc ctgagaagcg gatcatgatc gttctgatcc agcgagtggc cgaagcctcg
     6781 gtgagcgttg aaggacgcgt gaccggggcc gtcggccgcg ggcttcttgc gctggtgtgc
     6841 ggggagcccg gcgacacgcc cgccgtcatc gggaagctcg cacgcaagac cgcgagactt
     6901 cgcatcttcg aggacgaggc gggcaggatg aacaggagcg tcgtcgacgt cggcggcgat
     6961 gtgctctgcg tctcgcagtt cacgcttgcc gcggacacga tgtcgggcaa ccgcccgagc
     7021 ttctccagag ctgcgccgcc cgaagagggc cttgccgcct tcaatgcgtt tgtgacggcg
     7081 ctcaggggcg aggggctgtc ctgccccaca ggcgaattcg gcgcgcacat gcgcgtctcg
     7141 ctcgtcaatg acggaccggc gaccttcagc ctccacctta gggctgatgg tgctggcgcc
     7201 gactagtccc aatgctctag accgtggtct tgggccgaaa gtgatgatat aaatagagga
     7261 cgccgcccgc tccgggcggc tccgcacgtg gagaagcacc cggtgaaaat cacttccgac
     7321 aacttcaaac agggcgattt cattcctgaa gcccttgcct ggggccgaat cggagaggac
     7381 ggctcgaccg tccgctcgac gaacctcaat ccccagctct cctggacgga cgcgcccgag
     7441 ggcacggcgt ccttcgtgct tgcctgcctt gacgacgacg tgccgacgga tctcgccgag
     7501 cgcgacgcct cgggcgagat tcctgtttcc cagccccgcc gccgcttcgt gcattgggtg
     7561 cagcccaact gccccgctga cgtgacgagc atcgccgaag gggcgcttgc ggaggagagg
     7621 aagacgacgc cgggcttcgg cgccgtttgc atcaacgact acagccgcgg cggcacgcct
     7681 gctccgggcg aaacgggcac gggctacgat ggcccctgtc cgccgttctt cgacgcacgc
     7741 tggcactact accggttcat ggtcttcgcg ctcgacgccc cgaggctcga gcttcctgaa
     7801 aatgcgacat ggcaggacgt cgacgccgcc atgaagggcc atgtcctcgc gtcggctgag
     7861 cttgttggcc gctatacgct caatccccgt ctggccgccc tttgagacga ccggcattca
     7921 cagaatccac gtcattaacc tccggcggca tccgccgccg tcttactaaa ggaactgaag
     7981 tcgtgttcaa gcgctccaat accattgagg ccgtcgatcc cgccgtctgg gacgtcatca
     8041 gccacgagac cgcccgtcag gaagaccaca ttgagctcat cgcgagcgaa aactacgctt
     8101 ccccggcggt catgcaggcc cagggttcct gcctcacgaa caagtacgcg gaaggctatc
     8161 ccggcaagcg ctactacggc ggctgcgagc acgtcgacgt cgttgaacag ctcgccatcg
     8221 accgcgccaa gaagctcttc tgcgagcccg caggcgtcga gatggccgtc aacgtgcagc
     8281 cccactccgg cgctcaggcc aactccgccg tgtacttcgg ccttctcgag gtcggcgaca
     8341 cgatcatggg cctctcgctt gccgaaggcg gccatctgac gcacggcatg cacctcaact
     8401 attccggccg ctacttcaag gtcgttccct acggtcttga cgccaacgag gcgatcgact
     8461 acgaccgcgt tgaacagctc gccagggaat gcaagccgaa gatcatcgtc gccggcgcct
     8521 ccgcctactc gcttcgcatc gacttcaagc gctttgccga aatcgcccac tccgtcggtg
     8581 cctacctgat ggtcgacatg gcccactatg cgggcctcat cgccgccggc gtctacccga
     8641 ccccgttcgg ccacgccgac atcgtgacga cgaccacgca caagacgctc cgcggtccgc
     8701 gcggcggcct catcttctgc cgtcccgacc ttgagaagaa gatcaactcc gccgtcttcc
     8761 cgggcatgca gggcggtccg ctcatgcacg tcatcgccgc caaggccgtg gccctcggcg
     8821 aagcccttga cccgtccttc aaggtctacg gcgagcaggt gatgaagaac gccgctgcca
     8881 tggccgaaga gctcgtcaag cgcggcctgc gcatcgtttc cggccacacg gaaagccacg
     8941 tgatgcttgt cgaccttcgt ccgctcaaga tcacgggcaa ggccgccgaa acgctgctca
     9001 acgccgctca catcacggtc aacaagaacg ccatcccgaa cgatccggaa aagcccttcg
     9061 tgacgtccgg catccgtctc ggctctcccg ccatgacgac ccgcggcttc ggcgaagccg
     9121 aggcccgcaa ggtggcgaac ctgatcgtcg atctgctcga ggcgcctgaa gaccaggccg
     9181 tcttcgaacg cgtcaaggcc gaagtggcga acctcaccgc cgccttcccg gtctacgg
//
LOCUS       DBMDTI010000059         9207 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2097_length_9207_cov_2.367889, whole genome shotgun sequence.
ACCESSION   DBMDTI010000059 DBMDTI010000000
VERSION     DBMDTI010000059.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9207)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2097_length_9207_cov_2.367889"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="ACZPB6_04385"
     CDS             complement(<1..667)
                     /locus_tag="ACZPB6_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1183744.1"
                     /translation="MPDTQAIQAHWKTTLVILTLSAVLMSLSYTMLIPFLPMYLIEEL
                     GVEQSRANFWSGLVFSVSFLISGVMAPIWGAMADKRSRKLMALRAAILLAVSYALGGI
                     VQNEWQLLAMRCFQGFASGLWPACLAIMASTVPRERIGLAMGIMQGGMTAGGVLGPFF
                     GGALAELFSMRMTFFIGGAALAVITLLILFFIHEPPRKEQKKAAAAREKTSLLKIPVV
                     QRML"
     gene            complement(1329..1838)
                     /locus_tag="ACZPB6_04390"
     CDS             complement(1329..1838)
                     /locus_tag="ACZPB6_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183745.1"
                     /translation="MEARILKKVKRRAALCATCAAASLLAACSSTQEAAPEEPSYRSP
                     YQSYRSLCVVPAEEHSASFDLTLARMLRDRGFEPELLETGDLPSVRRCRGIVTFSTGR
                     TLRPFEAPASMSLTFLDAYTGETYHVAASRAKGGAGHALFADAPFSDPAQMIRQLVER
                     LFPERTDHE"
     gene            1968..2534
                     /locus_tag="ACZPB6_04395"
     CDS             1968..2534
                     /locus_tag="ACZPB6_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183746.1"
                     /translation="MSKNKSGAANRPALYNPTAACVLALLFTPIFGALLQARNWDALG
                     EHGRARASRMWVRTTLWLLFVFVIMQAVFQNEPFMQFGGLYFLVVTWASWMVTTGWKQ
                     IGYVRELFGSDYPRERLGRVTIFAGGCWVIYSMVSISIAMAIQLTGLDKMLPGAERAE
                     SQGVVLRVPEGSDKVVVEPLPVETRKAE"
     gene            2553..3608
                     /locus_tag="ACZPB6_04400"
     CDS             2553..3608
                     /locus_tag="ACZPB6_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019843.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1183747.1"
                     /translation="MPKRPDPRFPIPAPEKAEDYVLDLLGGIVRRRPLTGEEAMAQLI
                     RLEEDARRGNPAAQTEFARRLLADAPDSRKNRRALSLLRKAAARKLPEALHLLGVVML
                     RGQGLDADPAEGAKLLEEAAYLGSAGAAADLARLCCMGLGRPASDRDALYWYRIAGAK
                     GHTASQREAGLMLRDGRGARADNDEAARWLKAAAQGRDPQAQYELARLHQRRGWAGAD
                     PEEAARWLTEAALSGVVEAQYRLGVHYWAGRGGRVDLREAVRWTCRAAENGSTQALTT
                     LADFFLTGSALDMNRFNAYTLLKCAARLHDAGAATTLTALERMLTRSEKARATRLVMI
                     SKTPRVLVETLIPRQSR"
     gene            4351..4650
                     /locus_tag="ACZPB6_04405"
     CDS             4351..4650
                     /locus_tag="ACZPB6_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1183748.1"
                     /translation="MNRFDLVDRIAARNNISKAEADRVVVSVFEEIMSAVTMGDSVTL
                     IGFGSFRAVESAPRTGRNPATGEAIRIPAVRKPKFVAGTYFREIVNKYEGIKDEE"
     gene            complement(4791..5534)
                     /locus_tag="ACZPB6_04410"
     CDS             complement(4791..5534)
                     /locus_tag="ACZPB6_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1183749.1"
                     /translation="MNGHDRNGPAASELKLAGLTPFTTIDFPGRLSAVAFVQGCPWRC
                     VYCQNSWMQQRSFDPGLEHSSWEELESLLKRRHGLLDGVVFSGGEPCLDPALPAAVRA
                     VKEMGYEVGLHTGGSYPARLAEVLPDLSWVGLDVKAVPADGAHWAKVTGVESAQDKWT
                     ESLGLLLASGVPFECRTTAHPDYFSEAQLLETTAFLQARNVKDYALQICRKPPGLIAR
                     FGAVDPAWPSEPVLEKLRSGFERFTLRRD"
     gene            complement(5544..5741)
                     /gene="nrdD"
                     /locus_tag="ACZPB6_04415"
     CDS             complement(5544..5741)
                     /gene="nrdD"
                     /locus_tag="ACZPB6_04415"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR1183750.1"
                     /translation="MTNEVKKDVELTNEERQPCEVWTRVMGYHRPVQSFNTGKKGEFA
                     ERVCFTEKRAAEHDCKCRKAE"
     gene            complement(5786..7810)
                     /locus_tag="ACZPB6_04420"
     CDS             complement(5786..7810)
                     /locus_tag="ACZPB6_04420"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HIR1183751.1"
                     /translation="MEICERFAGKHIVKRNGSVKPFDVTKIVSAVTRAGKATGEFGEA
                     KALDLVCAYVLPRLDEKSTLCIELVQDAVEHALFEAGCFKTLRAYIVYRETRTKARDA
                     KQSWVNVESSINEYLDQIDWRVNANANQGYSLGGLILNVSGKVMANYWLNFIYPPEVG
                     RAHREADLHIHDLDMLSGYCAGWSLRTLLNEGLNGVAGKVEAAAPKHLSSATGQIVNF
                     LGTMQNEWAGAQAFSSFDTYLAPFIRKDNLPYAEVLQCMQELIYNLNVPSRWGTQTPF
                     TNLTFDWTCPGDLRAEHPMIGGETMPFTYGELQAEMDMINRAYIEVMLKGDAKGRVFT
                     FPIPTYNITPDFDWDSPNAMRLFDMTARYGLPYFQNFINSELKPNMIRSMCCRLQLDL
                     RELLKRGNGLFGSAEQTGSLGVVTVNCARLGFLFKDDEEGLLRRLDYLLELAKTSLEI
                     KRKVIQRHIDQGLFPYTKRYLGTLRNHFSTIGVNGINEMIRNFTSDREDITTDWGHAF
                     ALRLLDHVRARLVEFQEETDHMYNLEATPAEGTTYRFAKEDRKRFPGILQAGTPSNPY
                     YTNSSQLPVNFTDDPFEALERQDDLQRKYTGGTVLHLYMNEAVSSPEACRDLVRRTLT
                     RFRLPYITVTPTFSICPKHGYLSGRHDFCPKCDAELLAKKRAKAAREEAS"
BASE COUNT         1712 a         2945 c         2797 g         1753 t
ORIGIN      
        1 ggagcatgcg ctgcacgacg ggaatcttga ggaggctggt cttctcgcgc gcggcggccg
       61 ccttcttctg ctcctttctc ggaggctcgt ggatgaagaa taggatgagg agcgtgatga
      121 cggcaagtgc ggcgccaccg atgaagaagg tcatgcgcat cgagaagagc tctgcgagcg
      181 ccccgccgaa gaagggcccg agaacgccgc ccgcagtcat tccgccctgc atgatgccca
      241 tggccaggcc gatgcgctcg cgcggcacgg tgctcgccat gatcgcgagg caggcgggcc
      301 agaggcccga ggcgaacccc tggaagcaac gcatggccag cagctgccac tcgttctgga
      361 cgatgccgcc cagcgcatag gagacggcga ggaggattgc ggcgcgcagc gccatgagct
      421 tgcgcgagcg tttgtcggcc atggcgcccc agatcggggc catgacgcct gaaatcagaa
      481 aggagaccga aaagacgaga ccgctccaga aattcgcccg cgactgctcg acgccgagct
      541 cctcgatgag gtacatcggc agaaagggga tgagcatcgt gtagctgagc gacatcaaaa
      601 ccgcgctcag ggtcaggatg acgagcgtcg tcttccagtg ggcttggatc gcttgtgtat
      661 caggcacgtc ggatctccgt tgtgggtttc tggcttttct tcttcgtcct gccgccgagg
      721 ggaagcggac tcgacaataa tacgcccggg cgggagtcct gaacagcccg aaccttttgg
      781 cgagcccaaa agaaaaggga gcgtctggtc gaacgctccc gtaagatccg tgaaaagtga
      841 aagggggcat gccctttcgg gcgctccgga ggttcgcacg ggctggtctt cccgacgatc
      901 ctccatccct tcacaggcgg ccgctgcaac cggagagtca tgccggctgc gggccgtcgg
      961 gcagtcacgg actgccgatg gtccggcagc atctggtcgt ggcgctcctt tgcacatccc
     1021 ccgatgtaag cgccgatgcc gcctgcgtct gaagccgcct gatccggcct cattcgcctg
     1081 agtcgaacgg ttggttccgt tcaactgcac gcaaatttta gtgattttcg gcggaatttc
     1141 tactccttta ggctaactcc acaccccaat tccgcgcttc ttttgacgat aatcaggtaa
     1201 tttcacctgt ttacatctcg gttagggata tccccaagga tgtccttcta cccccaccga
     1261 ggtatgtctc ggaccacatg ccggtgcatg catgaaggcg aacgcccgcc ctcattctgt
     1321 ggaaaccctt actcatgatc ggtgcgctct ggaaacagtc tctccacgag ctgcctgatc
     1381 atctgagcgg gatccgagaa gggcgcatcg gcaaagagcg cgtgtcccgc gccgcccttt
     1441 gcacgggagg ccgccacgtg gtaggtctcg cccgtgtagg cgtcgaggaa ggtgagcgac
     1501 atggaggcgg gggcctcaaa gggtctcagg gtcctgcccg tcgagaaggt gacgatgccc
     1561 cggcagcggc gcaccgacgg caggtcgccc gtctcgagga gctcgggttc gaagccgcgg
     1621 tcgcggagca tgcgcgcgag cgtcagatcg aacgaggcgg aatgctcctc ggcgggcacg
     1681 acgcacagcg acctgtagga ctgatagggg cttcggtagg agggttcctc aggggccgcc
     1741 tcctgagtgc tcgagcaggc ggcgagcagc gaggccgcgg cacaggtcgc gcacagggcg
     1801 gcgcgccttt tcaccttttt caaaattcgt gcttccatcc ttctcctcat atatggatct
     1861 caatgacgat aggttacacc cactgaccgt agcccaaccc gtttgcgcgt acaattctcc
     1921 acttccgaac ccgcttccgc aaacaaccca tttccagtac gccgaccatg tccaagaaca
     1981 agtccggggc cgcgaaccgc cctgccctct acaatccgac cgccgcctgc gttctcgcgc
     2041 tcctcttcac gccgatcttc ggcgccctgc ttcaggcgcg caactgggac gcgctcggcg
     2101 aacacggccg cgcccgcgcc tcgcgcatgt gggtgcgcac gacgctctgg ctccttttcg
     2161 tcttcgtcat catgcaggcc gttttccaga acgagccctt catgcagttc ggcggcctct
     2221 acttcctcgt cgtcacctgg gcgagctgga tggtcacgac cggctggaag cagatcggct
     2281 acgtgcgcga gctcttcggc agcgactatc cccgcgagcg cctcggccgc gtgacgatct
     2341 tcgcaggcgg ctgctgggtg atctactcca tggtctccat ctcaatcgcg atggccatcc
     2401 agctcacggg cctcgacaag atgctccccg gcgccgagcg tgccgaaagt cagggcgtcg
     2461 tcctgcgcgt gcccgaaggg tccgacaagg tggtagtgga gccgctcccc gtcgagacca
     2521 gaaaggccga ataaggccct cctcacttcc gcatgcccaa gcgtcccgac ccgcgctttc
     2581 cgattcccgc ccctgaaaag gccgaggact acgtgctcga cctgctcggc ggcatcgtga
     2641 ggcgcaggcc cctcacgggc gaggaggcga tggcccagct catccgcctc gaggaggacg
     2701 cccgccgcgg caatcccgcc gcacagaccg aattcgcccg ccgccttctc gccgacgcgc
     2761 ccgacagccg caagaaccgc cgtgcgctct cgctcctcag gaaggccgcc gcccgaaagc
     2821 tccccgaggc ccttcacctg ctcggcgtcg tcatgctgcg cggccagggg ctggatgcgg
     2881 atcccgccga aggcgcgaag ctccttgagg aagccgccta tctgggcagc gcaggcgcgg
     2941 ccgccgacct cgcccgactc tgctgcatgg ggctcggacg ccccgcatcc gacagggacg
     3001 cactctactg gtaccgaatc gcgggcgcca agggacacac cgcctcccag cgcgaggcgg
     3061 gcctcatgct cagggacgga cgcggcgccc gcgccgacaa cgacgaggcc gccagatggc
     3121 tcaaggccgc cgcccagggg cgcgaccctc aggcccagta cgaactcgcc cgccttcacc
     3181 agcgccgagg ctgggctggc gccgatcccg aggaagccgc ccgatggctc accgaagccg
     3241 ccctttcggg cgtcgtcgag gcgcagtacc gcctcggcgt ccactactgg gcgggcaggg
     3301 gcggcagggt cgacctgcgc gaggccgtcc gctggacgtg ccgcgccgcc gaaaacggca
     3361 gtacgcaggc cctcaccacc ctcgccgact tcttcctgac gggctcggcc ctcgacatga
     3421 accgcttcaa tgcctacacg ctcctcaagt gcgccgccag gctccacgac gcgggcgccg
     3481 cgaccacgct caccgcactc gagcgcatgc tcacccgaag cgagaaggcc cgcgccaccc
     3541 gccttgtgat gatctcgaaa acccctaggg tcctcgtcga gaccctcatc ccccgccaga
     3601 gccgctgaaa gccgcccggt gctgcacaag gccctccctg cctgcgtaaa aaaaggtcag
     3661 aatccctgca ggacagggat ctgacctcaa caaccaaaag catatggatg acggacatga
     3721 ctccgcattg cttcgccggc atcggccgac agattcattc tgccaccgtc cgctctccct
     3781 ctcaatgcct ccaaaccgct ctcccggggc ttttgcctgc ccgacggcat gcgggatcaa
     3841 gtttcgcctc cctcttaaca cttttttcta cccctgaagt agcatgggat ttaccctaac
     3901 gacacttcaa ggcgaagcct cccgagcttt tctcgagtgc gtcccggaac agatcgagtg
     3961 ttttcaccta tgcagcggcg tacgggatca cctcccccaa cttccctgtg ccataggcgt
     4021 tgcaaggtca tatacaaaaa tccccttgcc ccgcaacagg ttgcgaaaca ccttcaccaa
     4081 taaaatcgcc cccctcgagg gacaatttcc tgtctttcgc tgcagggcag ggtttttgct
     4141 tgggtttacc tttggagtcc gcatcgctaa aatgcttggc atgcgaaatc acgggatctt
     4201 tcattcgcct ctaggagcgg cattttcgcc cttttgtcgt tttcaggcac tcccctgatt
     4261 cttcaacgca acccccaaaa atattcttcg tccccgcagc cacatccctt tcaactgacg
     4321 ggggcgggca gatccaccag gagtgttcaa atgaatcgtt tcgacctcgt tgaccgcatt
     4381 gccgcacgca acaacatttc caaagctgaa gctgaccgtg tcgtcgtgag cgtttttgaa
     4441 gaaatcatgt ccgccgttac gatgggcgat tccgtgactc tcatcggctt cggcagcttc
     4501 cgtgccgttg aaagcgctcc ccgcacgggc cgcaaccccg ccacgggcga agccatccgc
     4561 attccggcgg ttcgcaagcc gaagttcgtc gccggcacgt acttcaggga aatcgtcaac
     4621 aaatacgagg gcatcaagga cgaggaatga cggcagggct ctccaagcct tccgtctgag
     4681 ttgaagaagg accgcgtcgc acactgcgtc gcggtccttt tttcatagaa actcggcgcg
     4741 gaaacctctg tcttcagaca gggggtattg ccccatttga cggccttggc tcagtcccgc
     4801 ctgagcgtga agcgctcgaa tcccgagcgg agcttctcaa gcacgggttc ggagggccat
     4861 gcgggatcca cggcgccgaa ccttgcgatg agtcccggcg gcttgcggca gatctggagc
     4921 gcatagtcct tcacgttgcg cgcctgaaga aaggcggtcg tctccagaag ctgcgcctcg
     4981 ctgaagtagt cgggatgcgc cgtcgtgcgg cactcgaagg gaacgcccga agcgagaagc
     5041 agcccaaggc tctcggtcca cttgtcctgc gcgctctcaa cgcccgtcac cttcgcccag
     5101 tgagcgccgt cggcgggcac cgccttgacg tcgagcccca cccaggagag gtcggggagc
     5161 acctcggcga gcctcgcggg gtagctgccg cccgtgtgca gaccgacctc gtaccccatc
     5221 tccttcacgg cccgcacggc ggcggggagc gcaggatcga ggcagggctc gccgcccgag
     5281 aagacgacgc cgtcgagaag tccgtggcgc cgcttcagaa gagactccaa ctcctcccag
     5341 gacgaatgct cgagcccggg atcgaagctc ctctgctgca tccagctgtt ctggcagtag
     5401 acgcaccgcc agggacagcc ctgcacgaac gccaccgccg agagtctgcc cggaaagtcg
     5461 atggtcgtaa agggcgtgag gcccgcgagc ttcagttccg aagccgcggg cccgtttcgg
     5521 tcatgaccgt tcatgccgcg gccttattcg gccttcctgc acttgcagtc gtgttcggcc
     5581 gcgcgctttt ccgtgaagca gacgcgttcg gcgaactcgc ccttcttgcc ggtgttgaat
     5641 gactgcaccg gacggtggta gcccatcacg cgggtccaga cttcgcaggg ctggcgctct
     5701 tcattcgtga gttcaacgtc cttcttgact tcgttggtca ttttcgtgac tcctgataaa
     5761 aaagttgttt tcctttggag ccccctcagg aggcctcctc ccttgcggcc ttcgcgcgct
     5821 tcttcgccag cagctcggcg tcgcacttcg ggcagaagtc gtggcggccc gagagataac
     5881 cgtgcttggg acagatcgag aacgtcggcg tcaccgtgat gtacggcagg cggaagcgcg
     5941 tgagcgtgcg gcgcacgagg tcgcggcagg cctcgggcga ggagaccgcc tcgttcatgt
     6001 agaggtggag caccgtgccg cctgtgtact tccgttgcag gtcgtcctga cgctcgagcg
     6061 cctcgaacgg gtcgtccgtg aagttgacgg gcagctgcga ggagttcgtg tagtacggat
     6121 tcgagggcgt gcccgcctgc aggatgccgg ggaagcgctt gcggtcctcc ttggcgaagc
     6181 ggtaggtcgt gccctcggcc ggcgtcgcct cgaggttgta catgtggtcc gtctcctcct
     6241 ggaactcgac gaggcgagcg cgaacatggt cgagcaggcg cagcgcaaag gcatggcccc
     6301 aatccgtcgt gatgtcctcc ctgtcgctcg tgaagttgcg gatcatctcg ttgatgccgt
     6361 tgacgccgat cgtcgagaag tgattgcgca gcgtgccgag atagcgcttc gtgtacggga
     6421 agaggccctg atcgatgtgg cgctggatga ccttgcgctt gatctcgagg cttgtcttcg
     6481 caagctcgag cagatagtcg aggcggcgca gaagcccctc ctcgtcatcc ttgaagagga
     6541 agcccagacg cgcgcagttg accgtcacca cgccgagcga ccccgtctgc tccgcggagc
     6601 cgaagaggcc gttgccgcgc ttgagcagct cgcgcaggtc gagctgcagg cggcagcaca
     6661 tcgagcggat catgttgggc ttgagctcgg agttgatgaa gttctggaag tacggcagac
     6721 cgtagcgcgc cgtcatgtcg aagagcctca tcgcattggg gctgtcccag tcgaagtcag
     6781 gcgtgatgtt gtaagtcgga atcgggaacg tgaacacgcg tcccttcgca tcgcccttga
     6841 gcatcacctc gatgtaggcc ctgttgatca tgtccatctc ggcctgcagc tcgccgtagg
     6901 tgaacggcat cgtctcgccg ccgatcatcg gatgctcggc gcgcaggtcc ccgggacacg
     6961 tccagtcgaa cgtgaggttc gtgaacggcg tctgcgtgcc ccagcggctc ggcacgttca
     7021 ggttgtagat gagctcctgc atgcactgga gcacttcggc gtagggcagg ttgtccttcc
     7081 tgatgaaggg cgcgagatac gtgtcgaagc tcgagaacgc ctgggcgccc gcccactcgt
     7141 tctgcatcgt gccgaggaag ttgacgatct gacccgtggc ggacgaaagg tgcttcggcg
     7201 ccgcggcctc gaccttgccg gccacgccgt tgaggccctc gttgaggagc gtcctcagag
     7261 accagcccgc gcagtagccc gagagcatgt caaggtcgtg tatgtgcagg tcggcctcgc
     7321 ggtgcgcgcg gcccacctcg ggcggataga tgaagttgag ccagtagttg gccatcacct
     7381 tgccggagac gttcaggatg aggccgccga gcgaatagcc ctggttggcg ttcgcgttga
     7441 cgcgccagtc gatctggtcg aggtattcgt tgatggagct ttcgacgttg acccagctct
     7501 gcttggcgtc gcgagccttc gtgcgggtct cgcgatagac gatgtaggcg cggagcgtct
     7561 tgaagcaacc cgcctcgaag agcgcgtgct cgacggcgtc ctgaacaagt tcgatgcaca
     7621 gcgtcgactt ctcgtccaga cgcggcagca cgtacgcgca gacgagatca agggccttgg
     7681 cctcgccgaa ctcgcccgtc gccttgccgg cgcgcgtgac ggcgctcacg atcttcgtga
     7741 cgtcgaaggg cttcacgctg ccgtttcgct tgacgatgtg cttgccggcg aaccgttcac
     7801 aaatctccat ttgcttctcc attcttttcc tgtcacttcg ggcgaaacac gttcaaacac
     7861 tatatatagt atgtctcacc ccgccgaacc acgagctgta gtggtgccgc gcagaacggg
     7921 tctaatgact atagcaaaga gaatacggaa ccgcacccct ccattcgggg gatcccgggc
     7981 taccggcggc gcccgagaag acgcccctaa agcgccggaa cgcctgcggg caaaggattg
     8041 tcagtacatg cggagaggac gtgcggagtg cccgtcggcc caataggtga tttacctagt
     8101 gcatagagag tgccttatga gtagaatagg gcttagtcag acggttctgc ctaaccggat
     8161 ctcttgtttg tgcgtagaga gcatccgcag aattcgtcgg actcctgtct tcggcgtccg
     8221 aggggctgca tcggcaagat cagcgttgtc tttccgatgc aagcaagtat gccttgacag
     8281 ttttttcacc cgcaacacat ttgttgcggg tttttttatc ctttttttcc gccctctcct
     8341 ccgatccggg ccgtccttcc ccgtctagtc actttgtctc attcgactca cttcggcccc
     8401 ggcgaaaatc tgcgtatctt tacttacaag atcctgccta tgccaaacgc ccccaaagac
     8461 ggggaaaacc cgaaaaaagg ctcgatttca ggtgtttttt gcccaaaaaa acgcacgaaa
     8521 caatgacaat agtcaaattg tacgggcgcg tttacactta ttcgacgcat ttttcacccg
     8581 ccagcgcccg tcttcggccc gggatttcgc cctgaacagc cccctgggcc cgatcgtctt
     8641 ccgccctttt tcatagcggg aggctgcgcc cgcacgtcgg tgtctccctc tcaggatcat
     8701 gcgcgcccct gcggcgtcgt cggaggacgg agtgagggaa cgaagcgttc gacgtcgcgc
     8761 gtgagggcgc gtaaatgaag agaggggcat ggagggtgcg gagatgctca ggtccttcac
     8821 tggaagcgca ccgggcgtgc atgaggcctg catgaggggc gcacgaaggg cgaacgccgc
     8881 ccgcgcggca gtgcgcgacg gcacggaagg tgtgggagag aggaggacga ggatgcaaaa
     8941 aggcgcgccg cgaagcagcc cggctccgaa acgcctgcag gagaggcaca ggaaggaaat
     9001 caagtgcgaa gcgcggataa actaaagggg ctgatcggaa ctccctttca attccaatca
     9061 gccccctagc agaggaacgc ggagtgttaa cgaggcagga ccaaccacat cgtcaatctg
     9121 cgtttgagga ggagcaatga acttttgttt tcttgcttgc agataaagtt actcgcattt
     9181 acctcaggtt gcaaatgttc tgaaaac
//
LOCUS       DBMDTI010000060         9190 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2100_length_9190_cov_2.868742, whole genome shotgun sequence.
ACCESSION   DBMDTI010000060 DBMDTI010000000
VERSION     DBMDTI010000060.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9190
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2100_length_9190_cov_2.868742"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(327..974)
                     /gene="mobA"
                     /locus_tag="ACZPB6_04425"
     CDS             complement(327..974)
                     /gene="mobA"
                     /locus_tag="ACZPB6_04425"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HIR1183752.1"
                     /translation="MRKTAVAAPERANTIGLVLAGGRATRMGGVNKGLVLFNGEPMAG
                     RVIRTLAGQVSRVWVSANRDADAFVKLGAQKVFADVLEGFPGPLAALDSLNERLRTEG
                     TEGVEWILTVPCDVPLVPETLLEVFAGAFDGSPAYTIETGGYDQNTISLVHVSVLPTV
                     RPFLEGGDRKLGLWFERQGRGHVHWDGPKSDFYNVNAPQDLRQLESEAKTGALRR"
     gene            1057..2268
                     /locus_tag="ACZPB6_04430"
     CDS             1057..2268
                     /locus_tag="ACZPB6_04430"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR1183753.1"
                     /translation="MSKAIRADFDEVTRALREAVAPLDGACEAVPLLEAQGRILSESV
                     AAQFNFPHFDNSAMDGFALRTADLPETDSVKLPVCGIATAGHPFEEPLPECSALRIMT
                     GAPVPAGLDAVIPIEKVRVEDDFITFEPAGLRPGTNVRRQGEIFRAGEVLLTAPRRLT
                     RGDIGLAASLGYAKLSCRPVVRAAVFSSGDELVEPSDGRIYNANGAYVTLAARALGID
                     AEYAGILPDDDAVIEARFREAAATHDLVICSGGVGPGDCDFTAAVLNRLADLRHYHVA
                     LRPGKPFSFGYFKEAHTLILGLPGNPVASATSAENFLRTAVEALQGVHEKQLVLTARL
                     EGRIKSRAGRRDIVRGVLKTAEDGTLLFTPCPQQSSASLLSVSQSNAAAIVPEEAELL
                     QAGDAVRVVIP"
     gene            complement(2565..3620)
                     /locus_tag="ACZPB6_04435"
     CDS             complement(2565..3620)
                     /locus_tag="ACZPB6_04435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1183754.1"
                     /translation="MPEIFFCGDPHGTFDQINEAARLYSPDAMVILGDLQPPAPLHVV
                     LEEALAYTDIWWIPGNHDTDSDEFYDRLWRSELAGHNLHGRVACVAGMRIGGLGGVFR
                     GQIWMPDGNPNYYSPATFMRRVGQGNIWRGGVPRRHRSTIFPSVYGNLQRQKADILVT
                     HEAPSCHKKGFCALDRLAKTMGVKWLFHGHQHEDRAYGMQGLILTRAVGYNGIVNLKG
                     EVVVEAKLDPREEAALQATDEWRYMSDKNPIIVRDEDGHISLVPEGRECTPAILPVSE
                     KVLAKLKAAQEEGGKTPALEEKGRKAQKSEQGGAPQGVRKQGDEEKRGRSHRSSRSRS
                     GRDRWRRAKKPSQQQQG"
     gene            complement(3750..4715)
                     /locus_tag="ACZPB6_04440"
     CDS             complement(3750..4715)
                     /locus_tag="ACZPB6_04440"
                     /inference="COORDINATES: protein motif:HMM:NF052219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1183755.1"
                     /translation="MRRVLIDRLLSDKSVVTLQELQDVLKVSIPTIKRDLRYMRKELK
                     APIVYSRTRGGYLYDLPAGKSGASPEFQRFWFSAQELYVVVKMVDMLKALSKDKSSVI
                     LQDLEPLRSRVAGLLNLGGKSPREILGRVKVIDPSVIHGEPETFLTIGSALFEHKRVQ
                     ISYYSRTRRAESFREISAQRLVHYRNYWYVDAFCHATNALRTFAIDNIRRVIVLGTTA
                     KRVPLHTIEYNLDQSYGLFMSGDPKLAVLRFDAEASPYLKRETWHPRQTLEEHGDEVV
                     LTVPYTNPTELIGDIFRWREHVVVEAPEELRREVRECLLKTLSQY"
     gene            complement(4922..5560)
                     /gene="lolA"
                     /locus_tag="ACZPB6_04445"
     CDS             complement(4922..5560)
                     /gene="lolA"
                     /locus_tag="ACZPB6_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein periplasmic chaperone
                     LolA"
                     /protein_id="HIR1183756.1"
                     /translation="MTELTRRTFIIGLCLASAAALAAGKTEATGSLARLKAFLKSTTS
                     ARGTFRQTVIDRSGREVSKPSSGYFRFMRPGRFEWTYEKPYVQRIMSDGKTLWIHDPE
                     LFQVTEKALEGALPSTPAAILFGERDFEEDWNVTEISDTVLEARPKNPDGSFESVVIE
                     FDARGELAGMELVDTFAQRTTLVFGEIVRERMDPASFRLEIPEGTDVVKSAF"
     gene            complement(5561..7987)
                     /locus_tag="ACZPB6_04450"
     CDS             complement(5561..7987)
                     /locus_tag="ACZPB6_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR1183757.1"
                     /translation="MARETKKGTRKQKTKKDVRDQATEPVVQAAVVPGRDLKGLLCAW
                     FMNTIRTLWAWSWLLSVFFAIVIALTLGSYSLNDPSFSVSTGRTPENFCGALGAWTAD
                     LMLSAFGFSAWWFVLGSAMIGFFAIRTAWRRMKGETDPLRINPPKITAAVGFLAVLVG
                     STCLEALRLRRFQMILPGEPGGILGDSLAWGIRHYIGVGLSTVLFVSLIAVGLSLLMD
                     FQWGDVAERIGRFICCWFIDPVARLLKTKRREEVLAPQDEPETVDTDASIVPIVRVVD
                     DDTPPAAFVETLDRADAFSASVPASAGPAVQAAPRASKHADCPLSMSLLDEPPAHREA
                     NSADDLQMTGRLIVSKLKSYGIEASIGGIQPGPVITQYWLEPGPGVKGSQIDNVRDDL
                     RRTLGVQAVRVVPSIPNTSFIGLEVPNPVRETVRLKEILESKAFRESTAPLTLALGKD
                     IGGKAFVIDLAKMPHLLVAGTTGSGKSVGINAMILSMLYRNSPADLRLVLIDPKMLEF
                     SLYNGIPHLLCPVVTDMNKAASALKWLTREMDRRYAVMSRMGVRHFNNYNEKIRRAAE
                     NGETIPDPLQSPEDPVQKPLEVWPFIVCIVDELADLMLTNRKEVEGEITRLTQKARAA
                     GIHLIIATQRPSVDVVTSLIKANVPTRISFQVASGIDSRVILGESGAESLLGWGDMLL
                     RRPGVPQSTRIQGCFVADDEVLRVVTELQKLGEPDYIDSVTEEPDDEGGDGDAGAGGG
                     RRSGESDPLYDKAVDLVLRERRATISFVQRHLGVGYNRAANLLEAMEEARIVSKANSM
                     GRREILVPEN"
     gene            8281..>9190
                     /gene="trxB"
                     /locus_tag="ACZPB6_04455"
     CDS             8281..>9190
                     /gene="trxB"
                     /locus_tag="ACZPB6_04455"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR1183758.1"
                     /translation="MEAKHSKLIVLGSGPAGFTAAIYAARANLKPMLITGMDHGGQLM
                     TTSEVDNWPSAVDGIQGPELMERLQKQAERFGTEVAFDMITSVDLSKRPFTLKGDMGG
                     EYTCDALIIATGASAMYLGLESEEQYKGKGVSGCATCDGFFYRGKPVAVVGGGNAAVE
                     EALYLSNIASHVTLVHRRDTFRAEPIMVDHLMEAVREGRITLELDRTVKEVLGDGKGV
                     TGLELASTKGEADKTVDVEGVFIAIGHRPNTSLFEGKLELDRGYIVTAGTRSPAATAT
                     SVPGVFAAGDVQDQTYRQAVTSAATGC"
BASE COUNT         1676 a         3039 c         2759 g         1716 t
ORIGIN      
        1 ttgtcgctct tcgccggaat tgaaagagaa ggaattaacc ttcgtctttc ggcttttcct
       61 tcaaagcgcg aacttcgtaa gttttttgag tgcttttgaa agtcactctt gaaactgccg
      121 atgtccacac ttatcggttc gttgaattct gatttttaaa gaacagcgct ccgaagagca
      181 cagaattgag agtttatcag ctcatcaact ctttgtcaaa tttattcgcc gatgacttgg
      241 cgtcgtcagc gaaggattga aattaaacac ctttccgcag gctttgtcaa gactcgccgt
      301 aaaaataccg caaacacggt acttcctcac cttcggagag cccccgtttt cgcctcagat
      361 tccaactgcc gcaggtcttg aggagcattc acgttgtaaa agtcggattt ggggccgtcc
      421 cagtgcacat gccctcttcc ctgccgctcg aaccagagtc cgagctttcg gtctccgccc
      481 tcaaggaacg ggcgcaccgt aggaaggacc gacacatgga cgagcgagat cgtgttttgg
      541 tcgtatcccc ccgtctcgat cgtataggca ggactcccgt caaacgcgcc cgcgaacacc
      601 tcgaggagcg tctccggcac gagcggcacg tcgcacggca ccgtgagaat ccactcgacg
      661 ccctccgtcc cctcggtcct gaggcgctcg ttgagcgagt cgagcgcagc gagcggaccg
      721 gggaatccct cgagcacgtc ggcaaagacc ttctgcgccc cgagcttcac gaaggcgtcc
      781 gcgtccctgt tggccgaaac ccaaacgcgg gacacctgac ccgccagcgt cctgatgacc
      841 cgccccgcca tgggctcgcc gttaaagagc accagtccct tgttgacgcc ccccatccga
      901 gtggcccgtc cgcccgccag cacaaggccg atcgtattcg ccctttcggg cgccgcaaca
      961 gccgttttcc tcatggctag tctccgccgg cagcgctgct gccgctaaaa tccaagaatg
     1021 gccgcatcgg cggccgacac attgaaggat tttgacatgt cgaaagccat tcgtgccgac
     1081 tttgatgaag tcacccgcgc gctgcgcgag gccgtcgcac ctcttgacgg tgcctgcgaa
     1141 gcagtgcctc tgctcgaggc acagggacgc atcctctccg aaagcgtcgc cgcgcagttc
     1201 aacttccccc atttcgacaa ctcggccatg gacggcttcg cgctgcgcac ggccgacctg
     1261 cctgagacgg actccgtcaa gcttcctgtc tgcggcatcg caactgctgg ccatcctttt
     1321 gaagagcccc ttcccgagtg ctccgcactg cgcatcatga cgggcgcccc cgtgcccgcc
     1381 ggtctcgacg ccgtcattcc cattgaaaag gtccgcgtgg aggacgactt catcaccttt
     1441 gagcccgcag gcctcaggcc cggcaccaac gtgcgccgac agggcgagat cttcagggcg
     1501 ggcgaagtgc tcctcaccgc gccgcgccgc ctcacccgag gcgacatcgg cctcgccgcc
     1561 agtctcggct atgcgaaact ctcctgccgc cccgtggtca gggccgccgt cttctccagc
     1621 ggcgacgagc tcgtcgagcc ttccgacggg cgcatctaca acgccaacgg cgcctatgtc
     1681 acgcttgccg cccgcgcact cggcatcgac gccgaatatg caggcatcct ccccgacgac
     1741 gacgccgtga tcgaggcgcg ttttcgcgag gcggccgcca cgcacgacct cgtcatctgc
     1801 tcgggcggcg tgggtcctgg cgactgcgac ttcacagccg ccgtcctcaa ccgcctggcc
     1861 gacctgcgcc actaccatgt ggcgctgcgc cccggaaagc ccttctcgtt cggctacttc
     1921 aaggaggcgc acacgctcat tctcggcctg cccggcaatc ccgtcgcctc cgccaccagc
     1981 gccgagaact tcctgcgcac ggccgtcgag gccctgcagg gcgttcatga gaagcagctc
     2041 gtcctcacgg cccgcctcga gggacgcatc aagagccgcg ccggcagacg cgacatcgtc
     2101 cgcggcgtgc tgaagacggc cgaggacggc acgctccttt tcacgccgtg cccgcagcag
     2161 agctccgcct cgcttctgag cgtctcgcag agcaacgccg cagccatcgt ccccgaagag
     2221 gccgagcttc ttcaggcggg cgacgccgtg cgcgtggtca tcccgtagcc gtcaccgttc
     2281 cgccaaaaca agaatcgggt aggagcccgt ggattatttc cacgggcgtc cttccacacc
     2341 acgtggcgta cggttctcgt accacgcggt tgcgagcagt catcggagtg gcctccgtcc
     2401 tctctgctct tctgtttgca tcccctgttc cctttaccta cctcagtaga ccgctctgtt
     2461 ctgcagagtt ctcattcaga ggcgctcccg tgtagattgg gcccatgtct ggcacaccag
     2521 aaaaggcgcc gtcccactgg agacggcgcc tttttcgtca tagatcatcc ctgctgctgt
     2581 tgcgacggct ttttcgcacg ccgccagcgg tcccggccgc tgcgcgagcg agaggaacgg
     2641 tgcgaacgcc cccttttctc ctcatcgccc tgctttctca ctccctgcgg agcgccgccc
     2701 tgctcggact tctgggcctt cctgcccttt tcttcgagcg cgggagtctt tcccccttcc
     2761 tcctgagccg ccttgagctt ggcgagcacc ttttcggaca cgggcagaat cgcgggcgtg
     2821 cactcacgtc cttcgggtac gagcgagatg tgcccgtcct cgtctcggac gatgatcggg
     2881 ttcttgtccg acatatagcg ccactcgtcg gtcgcttgca gcgcggcttc ctctctcgga
     2941 tcgagcttcg cctcaacgac gacctcgccc ttgaggttca cgatgccgtt gtagccgact
     3001 gcacgggtga gaatcaagcc ctgcatgccg taggcgcggt cctcgtgctg gtgcccgtgg
     3061 aaaagccact tcacgcccat cgtcttggca agcctgtcga gtgcgcaaaa gcccttcttg
     3121 tgacacgagg gggcctcgtg cgtcacgaga atgtcggcct tctgcctctg aaggttgccg
     3181 tagacggacg ggaagatcgt cgagcggtgc ctgcgcggca cgccgccccg ccagatgttg
     3241 ccctggccca cgcgacgcat gaaggtcgcg ggactgtagt agttcggatt gccgtcaggc
     3301 atccagatct gaccacgaaa gacgccgccc agaccgccga tgcgcatgcc cgccacgcag
     3361 gcgacgcgcc cgtgcaggtt gtggcccgca agctcgcttc gccacagcct gtcgtagaac
     3421 tcgtccgaat ccgtgtcgtg attacccgga atccaccaga tgtccgtata ggcgagcgcc
     3481 tcctccagca ccacgtgcag cggcgccggc ggctgaaggt cgccgagaat gaccatggca
     3541 tcgggcgaat aaaggcgggc ggcctcgttg atctgatcaa acgtgccgtg cggatcgccg
     3601 cagaaaaaga tctcgggcaa gtcccggaca ctcgggcggc gatggcgcct gccgcgatgt
     3661 ctcctcgccc tgacctgctc ttccgtcatg attgaagttc ctgtctaccg tcaatccgga
     3721 agcgagccgc cctcaaggcg gcgtcagcct cagtattgac tcaatgtctt caggagacat
     3781 tcacggactt cgcggcgcag ttcttcagga gcctcgacga cgacgtgctc tcgccagcgg
     3841 aagatgtcgc cgatgagttc cgtcgggttc gtgtagggaa ccgtcaggac cacctcgtct
     3901 ccatgctcct cgagtgtctg cctcggatgc cacgtctcgc gcttgagata gggcgacgcc
     3961 tcggcgtcga acctgagcac ggcaagcttg ggatcgccgc tcatgaagag accgtagctc
     4021 tgatcgaggt tgtactcgat cgtatgaagc ggcacgcgct tggccgtcgt ccccaggacg
     4081 atgacgcggc ggatgttgtc gatggcgaac gtcctcaggg cgttcgtggc gtggcagaag
     4141 gcgtcgacgt accagtagtt tcggtaatga accagacgct gggccgagat ctcacgaaag
     4201 gactccgccc tgcgggtgcg gctgtaatag gaaatctgca cgcgcttgtg ctcgaacagg
     4261 gcgctcccga tcgtcaggaa ggtctcgggc tctccatgaa tcaccgacgg gtcgatgacc
     4321 ttcaccctgc cgagaatctc gcgcggactc ttgccgccga gattgagcag gcccgccacg
     4381 cgcgagcgca gcggttcgag atcctgaaga atgaccgaag acttgtcctt cgagagtgcc
     4441 ttgagcatgt cgaccatctt cacgacgaca tagagctcct gagcggaaaa ccagaacctc
     4501 tggaactcgg ggctcgcgcc ggacttgccc gctggaagat cgtaaagata gccgccgcgg
     4561 gttcgtgaat agacgatcgg cgccttcagc tccttgcgca tgtaacgcag gtcgcgcttg
     4621 atcgtgggaa tgctgacctt caggacatcc tgcagttcct gaagcgtgac gacgctcttg
     4681 tcggaaagca gtcggtcaat cagaactctg cgcatgctgt cctgcatatg ctggccaccc
     4741 aaaacggtcc gcctgagcag accttgctga tacttcttga aaaaaactgt gccgggtgaa
     4801 gccgtgattc gcctcccctg ctatgggatt cattgtatca gctcaacgga cgatcctcgg
     4861 tgaggctttc ccgtaccgat agagagggaa agcctctttt tgcccgcccg tccgaccgtg
     4921 ctcagaaggc ggacttgacg acgtccgtgc cctcgggaat ctcgagcctg aaggaggcgg
     4981 gatccatgcg ctcgcggacg atctccccga agacgagcgt ggtacgctgg gcgaacgtgt
     5041 cgacgagctc catgcccgca agctcgcccc ttgcgtcgaa ctcgatgacg accgactcga
     5101 acgagccgtc ggggttcttc ggacgcgcct cgagcacggt gtccgaaatc tccgtcacat
     5161 tccagtcctc ctcgaagtcg cgctcgccga agagaatagc ggcgggcgtg gacgggagcg
     5221 ccccctcaag ggccttctcc gtcacctgaa agagttcggg atcatgaatc cagagcgtct
     5281 tgccgtcgct catgatgcgc tggacgtacg gcttttcata cgtccattcg aagcggcccg
     5341 gacgcatgaa gcggaagtac cccgaggacg gcttcgagac ctcgcggccc gaacggtcga
     5401 tcaccgtctg cctgaaggtg ccgcgggcgc tcgtggtcga cttcagaaag gccttgagcc
     5461 tggcgagcga gccggtggcc tccgtcttcc ctgcggcaag ggccgcggcg gacgcaaggc
     5521 agagtccgat gatgaaggtt cggcgcgtca gttcagtcat ttagttctcc ggtacaagaa
     5581 tttcacgtcg gcccatgctg ttggccttgc tcacgatgcg ggcctcctcc atggcttcga
     5641 gaagattggc ggctcggttg tagccgacgc cgagatggcg ctgcacgaac gagatcgtcg
     5701 cgcggcgctc gcgaagcacg agatcgaccg ccttgtcgta aagcggatcg gactcgcccg
     5761 acctgcggcc gccgcccgca cccgcatcgc cgtcgccgcc ctcgtcgtca ggctcctcgg
     5821 tgacgctgtc gatgtagtcg ggctcgccga gcttctgcag ttcggtgacg acgcgaagca
     5881 cttcatcgtc agccacgaag cacccctgga tgcgggtcga ctgcggcacg ccgggacggc
     5941 gcaggagcat gtcgccccag ccgagcaggg actcggcgcc ggactcgccc agaatcacgc
     6001 ggctgtcgat gcccgaagcc acctggaagg agatgcgcgt gggcacgttt gccttgatga
     6061 ggcttgtcac cacatcgacc gacggacgct gggtcgcgat gatcagatga atgcctgctg
     6121 cacgggcctt ctgcgtgagt ctggtgatct cgccctcgac ctccttgcgg ttcgtgagca
     6181 tgaggtccgc gagttcgtcg acgatgcaga cgatgaaggg ccagacctcg agcggtttct
     6241 gaacgggatc ctcgggagac tgcagcggat cgggaatcgt ctcgccgttt tccgcggccc
     6301 tgcggatctt ctcgttgtag ttgttgaagt gacgcacacc catgcggctc atcacggcat
     6361 agcggcggtc catctcgcgg gtgagccact tgagggccga ggccgccttg ttcatgtcgg
     6421 tgacgacggg gcagagaaga tgcggaatgc cgttgtagag cgagaactcg agcatcttcg
     6481 ggtcgatcag cacgagcctg aggtccgcgg gcgaattgcg gtaaagcatc gagagaatca
     6541 tggcattgat gccaaccgac ttgcccgacc ccgtcgtacc agccacgagc agatgcggca
     6601 tcttggcgag gtcgatcacg aaggccttgc cgccgatgtc cttgccgagc gcgagcgtga
     6661 gcggcgccgt gctttctcgg aaggccttgc tctcgaggat ctccttgagg cggaccgtct
     6721 cccgcacggg gttcggaact tcaagaccaa tgaagctcgt gttcggaatg ctcggcacga
     6781 cgcgcacggc ctgaacgccg agcgtgcggc gcaagtcgtc cctcacgttg tcgatctgcg
     6841 aacccttgac accgggaccg ggctcgagcc agtactgggt gatcacggga ccgggctgga
     6901 tgcctccgat cgaggcctcg atgccgtagc tcttgagctt ggagacgatg agcctgcccg
     6961 tcatctgcag gtcgtcggcg ctgttggcct cgcgatgagc gggcggctcg tcgagaagcg
     7021 acatgctgag ggggcagtcc gcatgcttgg aagccctcgg ggcggcctgc actgcaggac
     7081 ctgcggaggc cggaacggat gcagagaacg catccgccct gtcgagcgtc tcgacaaatg
     7141 cggcgggcgg agtgtcatca tcgaccacgc gcacgatcgg cacgatgctc gcatccgtgt
     7201 ccacggtctc gggctcatcc tggggtgcga gcacctcctc gcggcgcttg gtcttgagga
     7261 gacgggccac gggatcgatg aaccagcagc agatgaagcg gccgatgcgc tccgcgacgt
     7321 ctccccactg gaagtccatc agaagcgaaa ggccgactgc aatgagcgag acgaaaagca
     7381 ccgtggagag tccgacgccg atgtagtggc ggatgcccca ggcgaggctg tcgccgagga
     7441 tgccgcccgg ctcgcccgga aggatcatct ggaagcggcg aagacgcagg gcctcgaggc
     7501 aggtcgagcc cacgagcacc gccagaaagc cgacggcggc cgtgatcttg ggaggattga
     7561 tgcgaagagg atccgtctcg cccttcatgc ggcgccaggc cgtgcggatc gcgaagaagc
     7621 cgatcatcgc gctgccgagc acaaaccacc aggcggaaaa gccgaaggcc gagagcatga
     7681 gatcggccgt ccaggcgccg agcgcgccgc agaagttctc gggcgtgcga cccgtcgaaa
     7741 ccgagaagga cggatcgttg agcgaatagc tcccgagcgt aagcgcgatc acgatggcaa
     7801 agaagaccga cagcagccag ctccatgccc agagggtcct gatcgtgttc atgaaccatg
     7861 cgcacagcag tcccttgagg tcgcggccag gaacgaccgc cgcctgaacg acaggctcgg
     7921 tcgcctgatc ccggacgtct ttcttcgtct tctgcttgcg agtgcctttt ttcgtttctc
     7981 ttgccatttt tacgcttgac gtgtagggag gcctgtctgc gcctcctcct gatccaccta
     8041 attctaaaaa gttcggccgc cgtcgtcggt ccctttgccg ccgcggcagg cagccggcca
     8101 tgcatggaaa tcgttacagc gtattgcccc gcccgcgaag gccgggacgc cttaagacat
     8161 cactattaat tttaggtcta aaatcaaatt catctatcat tttgcatgag ggcgccccga
     8221 agagtgcgcc cctcatgccg cctgcgctcc aaaggcgcag gtccaccgga gatttttccc
     8281 atggaagcca aacacagcaa gctcatcgtc ctcgggtccg gccccgccgg cttcaccgcc
     8341 gccatctacg ccgctcgcgc caacctcaag cccatgctca tcacgggcat ggaccacggc
     8401 ggtcagctca tgacgacctc cgaagtcgac aactggccct ccgccgtcga cggcatccag
     8461 ggccccgagc tcatggaacg ccttcagaag caggccgagc gcttcggcac cgaggtcgcc
     8521 ttcgacatga tcacctccgt cgatctttcc aagcgtccct tcacgctcaa gggcgacatg
     8581 ggcggcgaat acacctgcga cgcgctcatc atcgcgacgg gcgcctccgc catgtacctc
     8641 ggtcttgaaa gcgaggagca gtacaagggc aagggcgtct cgggctgcgc cacgtgcgac
     8701 ggcttcttct accgcggcaa gcccgtcgcg gttgtgggcg gcggcaatgc agccgtcgag
     8761 gaggccctct acctctccaa catcgccagc cacgtcacgc tcgtgcaccg ccgcgacacc
     8821 ttccgcgccg aacccatcat ggtcgaccac ctgatggagg ccgtcaggga aggccgcatc
     8881 acgctcgagc tcgaccgcac agtcaaggaa gtgctcggcg acggcaaggg cgtcacgggt
     8941 cttgagctcg cctccacgaa gggcgaagcc gacaagacag tcgacgttga aggcgtcttc
     9001 atcgccatcg gccaccgtcc gaacacgtcc ctcttcgagg gcaagctcga gctcgaccgc
     9061 ggctacatcg tcacggccgg cacgcgcagc cccgccgcta ccgccaccag cgttcccggc
     9121 gtcttcgccg caggcgacgt gcaggaccag acctaccgtc aggccgtcac ctccgccgcc
     9181 acgggctgca
//
LOCUS       DBMDTI010000061         9166 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2105_length_9166_cov_4.295753, whole genome shotgun sequence.
ACCESSION   DBMDTI010000061 DBMDTI010000000
VERSION     DBMDTI010000061.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9166)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9166
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2105_length_9166_cov_4.295753"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="ACZPB6_04460"
     CDS             <1..219
                     /locus_tag="ACZPB6_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1183759.1"
                     /translation="VLLLAGLPPGDAISAAWAMLSNIGPGLGGVGPTGNYADLSDIQL
                     SVCSFLMLIGRLEIFTVFAIFTRNFWRI"
     gene            complement(253..624)
                     /locus_tag="ACZPB6_04465"
     CDS             complement(253..624)
                     /locus_tag="ACZPB6_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HIR1183760.1"
                     /translation="MSLVLFSCRATGPFIMFEETARRVFKVLGRPWTETGAFSCDELP
                     EILRILDEEARKDKERLAQIEAERERKLRESTYDEELRMREEEKRNSVEKINFFQRVV
                     PLQSMIKRAIKHGDAIMWGRP"
     gene            710..1147
                     /locus_tag="ACZPB6_04470"
     CDS             710..1147
                     /locus_tag="ACZPB6_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1183761.1"
                     /translation="MSAGICIIAHEPLASALKSCARHIFSATGDTFCDSIAVFDVPCD
                     VDAEQGEAEARRLISNFPANQGVLVFTDVLGATPSNIAHRLLDNPQVRVITGVNLPAL
                     ITALSHHEECAARIAVIAEGAARGGISTSCGKPSAIKDTVNAD"
     gene            1137..1406
                     /locus_tag="ACZPB6_04475"
     CDS             1137..1406
                     /locus_tag="ACZPB6_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1183762.1"
                     /translation="MQTKQLTVLNKLGLHARPCALLAKTAGFFKSSITLTHNNHSVDA
                     KSILGLMTMAVPAGTTLTVTADGPDEDSAIKDITALFDNKFGEQA"
     gene            1422..3284
                     /gene="ptsP"
                     /locus_tag="ACZPB6_04480"
     CDS             1422..3284
                     /gene="ptsP"
                     /locus_tag="ACZPB6_04480"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR1183763.1"
                     /translation="MSDEPLVASSTVEEQAPPPEKLTGISVYPGIAYGLCQQFSAGDL
                     EIPQFVIEKTGTRGEIQRLRAAIATVDKQLGALIDSADEDMPAEAAAFIDVHRTILRD
                     PTLIEETVDIIKEKLVNAEWALSLRLEKTRRDFESIEDDYIRERIKDIAHVVLRVQRV
                     LTGRRSASSLLEAEGLDETIILVADQLDPADMLLLRGRDDLDIAGIILEEGSITSHAV
                     ILARSLEIPTLVGVKGACEILETDVPVLLNADEELIDLSPSAEKRRNARQRMKLLTAE
                     KKRLKKLKQFDAVTLDGHTIKLFANIALPEDVKDVHRAGADGVGLFRTEFLFMNRDDL
                     PCEDEQYNAYCRVIRSMKGQPVVIRTADLGGDKVMSQDALEKLTTGPLEELNPSLGLR
                     GLRFCFAHPKLFVTQLRAILRAAATGSGNVRILLPMVTSPEDVSRVREFISLACSELA
                     AEGQKFTADVPLGGMVEVPAAVMMLKDLIPVLDFFSLGTNDLVQYTLAVDRTNAAVSK
                     YYDECHPSVLRMIATTVRQVHQAGKSVSVCGEMAARPELVPFFIGLGCTRLSMTLTQI
                     PAIKKRILKTDRAKAEVFAASVLRRRSAKAVHQSFIQEEEAFQNRKVADPSTTL"
     gene            3281..3973
                     /locus_tag="ACZPB6_04485"
     CDS             3281..3973
                     /locus_tag="ACZPB6_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA/YgfB family protein"
                     /protein_id="HIR1183764.1"
                     /translation="MSNSYEKPLNDDEFVELGELLAAMPEPFEPMEPDYMDGFLTALL
                     CLPDEPSPGDWMPYIFDSQARQDAALADPDEQDRLEELIYRRYRSIDTTLARCRPIDP
                     IIYEIEDNRGRPVRGADSVAAVIPFALGFSEVINRWEGLKDSTDDRINGALLGILRHL
                     PDDVAGDLAEIKADLDLESPIENLDQALEDIAESVAEIAAVTRGFEKKEEPPKKKPAP
                     KGPRRPQGGRRH"
     gene            complement(3983..5320)
                     /locus_tag="ACZPB6_04490"
     CDS             complement(3983..5320)
                     /locus_tag="ACZPB6_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HIR1183765.1"
                     /translation="MNVRALISDFRVYLQPQPVRMFLLGFSSGLPLLLILGTLSFWLR
                     EAGVDLKTIGFFSWIGLVYGFKWVWAPAVDRLRIPVLGRLLGRRRSWLLLSQAGVIAA
                     LAGMAVSDPLHHLQGIMVFALMCAFFSATQDIALDAYRIESADSNLQAVLAAMYQTGY
                     RLAMIWAGAGTLALAAWSAGGETGYFPHAWEFAYLGMAASMCVGVLTVLFVKEPEAPV
                     RPVEAVSRSWPERFAINVVAPFRDFFVRFGWGAAGILLLIATYRISDIVMGVMANPFY
                     SDTGFTKEEIATVSKVFGVVMTLAGAFVGGIVTMRLGILKTMAVGAVLAAVTNLLFAA
                     LAGIGHNVGFLIFTVSADNLAAGLASAAFIAYLSSLTNKAYSATQYALFSSLMLLLPK
                     FIAGFSGVMVESLGYQGFFLLTASMGVPVILLVWWVSRQARARELMSGGRAEK"
     gene            complement(5321..6010)
                     /locus_tag="ACZPB6_04495"
     CDS             complement(5321..6010)
                     /locus_tag="ACZPB6_04495"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HIR1183766.1"
                     /translation="MLLKDVLFSQGFGTRYDCAAMVASGAVEIAAEVHDDPDEDVETE
                     GLVFRCLGTDWPYFEKAIIALNKPAGYECSMKPSAHPSVMTLLPSPLRRRNMQPVGRL
                     DVDTTGVLIFTDDGALQHRLIHPKRHVSKIYDVVCRHPVDEKTREKLMAGVVLDDDPK
                     PVAAKDVEILDEHHLRMTLVQGKYHQVKRMIAACSNRCDGLHRSAFGLYVMPETLKQG
                     EWVWLTKKDLV"
     gene            complement(6014..6796)
                     /locus_tag="ACZPB6_04500"
     CDS             complement(6014..6796)
                     /locus_tag="ACZPB6_04500"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR1183767.1"
                     /translation="MFRIVTFNANGVRSALTKGFFNWFQAQNADVLCIQELKAQEKDI
                     PAEVAGLPGYQAFFHCAEKKGYSGCGIWCRRKPDEVRYGFGDPEFDAEGRYVEAIFGD
                     LSVISCYFPSGSSSEERQEAKFRFLASFLKHMDELRTSGRRVILCGDVNIAHKEIDLK
                     NWKGNVKNSGFLPEERQWLTDLFEKHGWRDVFRLVDARPEQYTWWSQRGQARAKNVGW
                     RIDYHLATENVAAAARAASIYPDEKFSDHAPLTIDYDFSLED"
     gene            6911..7588
                     /gene="pyrE"
                     /locus_tag="ACZPB6_04505"
     CDS             6911..7588
                     /gene="pyrE"
                     /locus_tag="ACZPB6_04505"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR1183768.1"
                     /translation="MQPIQEQFIEFALEADVLRFGEFKTKAGRLSPYFFNAGLFNTGA
                     LLDRLGEFYAKTLLAARDAGRIEFDMIFGPAYKGIPLAASVAMNLSRLGCDVPWAFNR
                     KEAKDHGEGGVIVGAPLKGKVVIIDDVISAGTSVRESVKIIQANGAVPSGVLIALDRQ
                     ERGGNAVEMTETSAVQDVEKTLGMNVASIICLNDLLAFMNGDTDMAKAVSCHKEAVAA
                     YRARYGA"
     gene            complement(7685..9136)
                     /gene="gatB"
                     /locus_tag="ACZPB6_04510"
     CDS             complement(7685..9136)
                     /gene="gatB"
                     /locus_tag="ACZPB6_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR1183769.1"
                     /translation="MQWEVVIGLETHVQLSTRSKIFSGAARHFGDEPNVNACYVDLAL
                     PGSLPVLNHSAVEKAIRFGLAIDATVANHSVFDRKNYFYPDLPKGYQISQFELPVVLG
                     GKLTFPVQPKKGDPYVKTVNLTRAHLEEDAGKSIHGLVQGCSGIDLNRAGTPLLEIVT
                     EPEMRSAAEAVGYARALHALVVWLGISDGNMQEGNFRCDANVSVRPVGQKEFGTRCEI
                     KNLNSFRFLQEAIDYEVRRQIELIEDGGKVVQATRLYDPDKGETRQMRTKEDSMDYRY
                     FPDPDLLPLEISDEWIERVRGEMPELPHQLCERLQSEFGLPQYDASMLTASRDIADYY
                     VALAAKVSDRKMAANWVMGELSAAVNGVEGMTFGKAPVTPEILAAILGRILDNTVNQK
                     GAKQIFAAVWEGEGTDVDALIESKGLKQISDTGALEGIIDEVLAANQKSVDEFRAGKQ
                     KAFNALVGQCMKATRGKANPAQVNELLRKKLGA"
BASE COUNT         1830 a         2987 c         2729 g         1620 t
ORIGIN      
        1 gttctgcttc tggcgggact cccccccggg gacgccatca gcgccgcatg ggccatgctt
       61 tcgaacatcg gcccgggcct cggcggcgtc ggcccgacgg gcaactatgc ggacctgagc
      121 gacatccagc tgtccgtctg ctccttcctg atgctcatcg ggcgcctgga gatcttcacc
      181 gtgttcgcga tcttcacgcg gaacttctgg cgaatctgat gcaggcggtg aggccgcctg
      241 cgacgtgcag tcctacggac ggccccacat gatggcgtcg ccgtgcttga tggcgcgctt
      301 gatcatcgac tggagaggca cgacgcgctg aaagaaattg atcttctcga cggagttcct
      361 cttctcctcc tcgcgcatgc ggagctcctc gtcgtaggtc gactcgcgaa gcttcctttc
      421 ccgctcggcc tcgatctgcg caaggcgctc cttgtccttg cgggcctcct catcaaggat
      481 cctcaggatt tcgggcagct cgtcgcagga gaaggcgcct gtttcggtcc acgggcggcc
      541 gagcaccttg aagacccgcc tggccgtttc ctcgaacata atgaacggcc ctgtggcacg
      601 gcatgaaaaa agcaccagag acatagaaga cactcccgat cacaaccttg ggttacacta
      661 cttcgcataa catacccact ggattttatc gtacctaacg gagccttctg tgtctgcagg
      721 gatctgcatc atcgcccacg agccgctggc cagcgcattg aaaagctgtg cccggcacat
      781 cttcagcgcc acgggcgaca ccttctgcga ctccatcgca gtcttcgacg tgccctgcga
      841 cgtggacgcc gagcaaggcg aggcggaagc ccgcaggctc atctcgaact tccccgcgaa
      901 tcagggcgtg ttggtcttca ccgacgttct cggcgcgacg ccttccaaca tcgcccatcg
      961 tcttctggac aatccgcagg tgcgcgtcat cacgggcgtc aatctgcccg cactcatcac
     1021 cgctctcagt catcacgagg aatgcgcggc ccgcattgcc gtcatcgccg agggagccgc
     1081 gcgaggcggc atctccacgt cctgcggcaa gccttccgcc atcaaggaca ctgtcaatgc
     1141 agactaagca gctcaccgtg ctcaacaagc tcgggctcca tgcccgtcct tgcgccctgc
     1201 tcgccaagac cgcaggcttc ttcaaatcct ccatcacgct cacgcacaac aaccacagcg
     1261 tggatgccaa gtcgattctc ggcctcatga cgatggccgt gcccgcaggc acgacgctca
     1321 ccgtcacggc ggacggtccc gacgaagaca gcgccatcaa ggacatcacc gcccttttcg
     1381 acaacaagtt cggcgaacaa gcctaagacg gagaatccac catgagtgac gaacccctcg
     1441 ttgcgtcctc cacagtcgaa gaacaggcac cgcctcccga aaagctgacg ggcatcagcg
     1501 tctatcccgg catcgcctac ggcctctgcc agcaattctc cgccggcgat ctggagatcc
     1561 cgcagttcgt catcgaaaag accggaaccc gcggcgaaat ccagcgcctg cgcgccgcca
     1621 tcgccaccgt cgacaagcag ctcggcgccc tgatcgactc cgccgacgaa gacatgccgg
     1681 ccgaggcggc ggcgttcatc gacgtgcacc gcaccatcct tcgagacccc accctgatcg
     1741 aggagaccgt cgacatcatc aaggaaaagc tcgtcaacgc cgaatgggcg ctgtcgctgc
     1801 gccttgaaaa gacgcgacga gacttcgagt ccatcgagga cgactacatc cgcgaacgca
     1861 tcaaggacat cgcgcacgtc gtcctgcgcg tgcagcgcgt gctcacgggc aggcgctccg
     1921 cctcaagcct tcttgaagcg gaaggccttg acgagacgat cattctcgtg gccgaccagc
     1981 tcgatcccgc cgacatgctg cttctgcgcg gccgagacga cctcgacatc gcgggcatca
     2041 tcctcgagga aggcagcatt acgagtcatg ccgtcattct tgcgcgctcg cttgaaattc
     2101 cgactctggt cggcgtcaag ggcgcctgcg agatcctcga gaccgacgtg cccgtcctgc
     2161 tcaacgcgga cgaagagctc atcgacctga gcccctccgc cgaaaagcgc aggaacgcac
     2221 gccagcgcat gaagcttctg accgccgaga agaagcgcct caagaagctc aagcagtttg
     2281 acgcggtcac gctcgacgga cacacgatca agctcttcgc caacatcgcg ctccccgagg
     2341 acgtcaagga cgtccatcgc gcgggtgcgg acggcgtcgg gctcttccgc acggaattcc
     2401 tcttcatgaa ccgcgacgac cttccctgcg aagacgagca gtacaacgcg tactgccgcg
     2461 tcatccgctc gatgaagggc cagcccgtcg tgattcgcac ggccgacctc ggcggcgaca
     2521 aggtcatgtc gcaggacgcc ctcgaaaagc tcaccaccgg accgctcgag gagctgaatc
     2581 cctcgctcgg cctcaggggc ctgcgcttct gctttgcgca tccgaagctc ttcgtcacgc
     2641 agcttcgcgc aatcctgcgc gccgccgcca cggggtcggg caatgtcaga atcctgctgc
     2701 cgatggtcac atcgcccgag gacgtcagca gggtgcgcga attcatctcg ctggcctgca
     2761 gcgaacttgc cgccgaaggc cagaagttca ccgccgacgt tccgctcggt ggaatggtcg
     2821 aggttcccgc cgccgtcatg atgctcaagg acctgattcc cgtcctcgac ttcttctcgc
     2881 tcggcaccaa cgacctcgtg cagtacacgc tggccgtcga ccgaacgaat gcagcggttt
     2941 caaagtacta cgacgagtgc cacccgagcg tgctgcgcat gatcgccacg acggtcagac
     3001 aggtccacca ggcaggcaag agcgtgtccg tatgcggtga aatggctgcc cgaccggaac
     3061 tcgtcccctt cttcatcgga ctcggatgca cgcgtctttc catgacgctc acccagattc
     3121 ccgccatcaa gaagcgaatt ctgaagaccg acagggccaa ggccgaagtc ttcgcagcca
     3181 gcgtgctgcg acgccgatcc gccaaggccg tccatcaatc ctttattcag gaggaggagg
     3241 ccttccagaa caggaaggtc gccgatccgt ccactaccct atgagcaatt cctacgaaaa
     3301 accgctgaac gatgacgagt tcgtcgaact cggcgaactg cttgccgcca tgcccgaacc
     3361 gttcgagccg atggagcccg actacatgga cggcttcctc accgcgcttc tgtgcctgcc
     3421 cgacgagccg tctcccggcg actggatgcc ctacatcttc gactcccagg cccgtcagga
     3481 cgccgctctg gcggatcccg acgaacagga ccgtctggag gaactcatct accgccgcta
     3541 ccgctccatc gacacgacgc ttgcgcgctg ccgccccatc gatccgatca tctacgagat
     3601 cgaggacaac cgcggccgtc ccgtgcgggg cgccgacagc gtggccgccg tcattccgtt
     3661 cgccctcggc ttctccgaag tgatcaaccg ctgggaaggt ctcaaggaca gcacggacga
     3721 ccgcatcaac ggcgccctgc tcggtatcct ccgtcatctg cccgacgacg tggcgggcga
     3781 ccttgcggaa atcaaggcgg accttgatct ggaaagcccg atcgagaacc ttgaccaggc
     3841 gcttgaagac atcgcggaaa gcgtcgcgga aatcgcagcc gtcacccgcg gcttcgaaaa
     3901 gaaggaggag cctcccaaga agaagcccgc tccgaagggc ccgcgccgtc cgcagggcgg
     3961 ccgacgccac tgaggcacga gactacttct ccgcgcgccc gccgctcatc agctcccggg
     4021 cgcgcgcctg cctgctcacc caccagacaa gcaggatcac gggcacgccc atcgatgccg
     4081 tcaggagaaa gaagccctga tagccgaggc tctcgaccat tacgcccgaa aagcctgcga
     4141 tgaacttcgg cagaagcagc atcaggctgc tgaagagcgc gtactgcgtc gcagaatagg
     4201 ccttgttcgt aaggcttgag aggtacgcga tgaaggccgc gctcgcaaga cctgccgcaa
     4261 ggttgtcggc ggacaccgtg aagatgagaa atcctacgtt gtgaccaatg ccggccagtg
     4321 cggcgaagag aaggttcgtc acggcggcaa ggaccgcacc gacggccatc gtcttgagaa
     4381 ttcccagacg catcgtcacg atgccgccca cgaaagcacc cgcgagcgtc atcacgacgc
     4441 cgaacacctt ggagaccgtc gcgatctcct ccttggtgaa gcccgtgtcc gaatagaagg
     4501 ggttggccat cacgcccatg acgatgtcgg agatgcggta ggtggcgatc aacagcagaa
     4561 tgcccgccgc cccccagccg aagcgcacga agaagtccct gaagggcgcg acaacattga
     4621 tagcgaagcg ctcgggccac gagcggctca cggcttctac gggccggacg ggtgcctcgg
     4681 gctccttcac gaagagcacc gtcagcacgc ccacgcacat ggacgccgcc atgccgagat
     4741 aggcgaactc ccaggcatgc ggaaagtagc ccgtctctcc gcctgcgctc caggccgcca
     4801 gcgcgagcgt gcctgcgccc gcccagatca tggccagccg atagcccgtc tggtacatgg
     4861 cggcgagcac ggcctgcaga ttcgagtccg cagactcgat gcggtaggcg tcgagcgcaa
     4921 tgtcctgtgt cgccgaaaag aacgcgcaca tgagcgcaaa gaccatgata ccctgcaggt
     4981 gatgcagggg atccgacacc gccatgcccg ccaacgccgc aatgacgccc gcctgggaca
     5041 gcaggagcca ggagcgacgg cgcccgagca ggcgcccgag cacgggaatg cgcagacggt
     5101 cgacggccgg cgcccacacc cacttgaagc cgtagacgag tccgatccag ctgaagaagc
     5161 cgatggtctt cagatcaacc cccgcctccc tcagccagaa gctgagcgtg cccagaataa
     5221 gcagcagggg aagtcccgag ctgaagccaa gcaggaacat cctgacaggc tgaggctgca
     5281 ggtagacacg aaagtcggat atcagcgccc tgacgttcat ttagaccaga tctttcttcg
     5341 tcagccagac ccattcgccc tgcttgagcg tctcgggcat cacgtagagg ccgaaggcgg
     5401 agcggtgaag gccgtcgcag cggttcgagc acgctgcgat catgcgcttg acctgatggt
     5461 acttgccctg cacgagcgtc atgcgaagat ggtgttcgtc aaggatctcc acgtccttgg
     5521 cggccacggg cttgggatcg tcgtcgagca cgacgcccgc catgagcttt tcgcgagtct
     5581 tctcatccac gggatgcctg cagacgacgt cgtagatctt cgagacgtgg cgcttgggat
     5641 ggatgagacg atgctgcagc gcgccgtcgt ccgtaaagat cagcacgccc gtcgtatcga
     5701 cgtcgagacg gccaacgggc tgcatgttgc ggcggcgaag cggacttggc agcagcgtca
     5761 tgacggaggg atgagcggaa ggcttcatcg agcactcgta gcccgcaggc ttgttgagcg
     5821 caatgatcgc cttctcaaag tacggccagt cggtgccgag gcagcggaag acgagacctt
     5881 cggtctcgac gtcctcgtcg ggatcgtcgt gcacttcggc tgcgatttca acggcaccgg
     5941 aggccaccat ggccgcacag tcatagcgcg tgccgaagcc ctgggaaaaa agcacgtcct
     6001 tgagcagcat tcgtcagtcc tcgagagaaa agtcgtagtc gatggtgagc ggcgcatgat
     6061 ccgagaactt ctcgtccgga taaatcgacg cggcgcgggc ggcagccgcc acgttttcgg
     6121 tggccagatg atagtcgatg cgccagccga cgttcttggc acgcgcctga ccgcgctggc
     6181 tccaccaggt gtactgctcg ggtctggcgt cgacaagacg gaagacgtcg cgccaaccgt
     6241 gcttctcgaa cagatccgtc agccactggc gttcctcggg aagaaagccc gagttcttga
     6301 cgttgccctt ccagttcttg aggtcgatct ccttgtgggc gatgttcacg tcgccgcaga
     6361 gaatgacgcg acggccggag gtcctgagct cgtccatgtg cttcaggaag gatgcgagga
     6421 agcggaactt cgcctcctga cgttcctcgg acgaggagcc cgatgggaaa tagcaggaga
     6481 tgacgctgag atcaccaaag atcgcctcaa catagcggcc ttcggcatca aactcgggat
     6541 cgccgaatcc gtagcggacc tcgtcgggct tccgacggca ccagatgccg cagcccgagt
     6601 agcccttctt ttcggcgcag tggaagaagg cctgatagcc gggaagcccg gcgacctcgg
     6661 cagggatgtc cttctcctga gccttgagtt cctggatgca gagcacgtcc gcgttctggg
     6721 cctgaaacca attgaagaag cccttggtca gtgcggaacg cacgccgttg gcgttgaaag
     6781 tcacgatacg gaacatcgga gtcctctaga aacaagattc gaatggttaa ggataccgag
     6841 gtttagcagt acaatgccac acgatcccgc gaggtcgtgc gacctctttt tcccttttga
     6901 aaaaacagtc atgcaaccga ttcaagaaca gttcatcgaa tttgccctgg aagccgacgt
     6961 ccttcgcttc ggcgagttca agaccaaggc cggccgtctc tctccgtact tcttcaacgc
     7021 cggcctcttc aacaccggtg ccctgctcga ccgcctcggc gagttctatg cgaagacgct
     7081 tctggctgcc cgcgacgccg gccgcatcga gttcgacatg attttcggtc ccgcctacaa
     7141 gggcatcccg ctggccgcct ccgtggccat gaatctctcc cgcctcggct gcgacgtgcc
     7201 ctgggccttc aaccgcaagg aagccaagga ccacggcgaa ggcggcgtga tcgtcggcgc
     7261 cccgctcaag ggcaaggtcg tgatcatcga cgacgtgatc tccgccggca cctccgtgcg
     7321 cgaatccgtc aagatcatcc aggccaacgg tgccgtcccc tccggcgtgc tgatcgccct
     7381 tgaccgtcag gaacgcggcg gcaatgccgt cgaaatgacg gaaacctccg ccgttcaaga
     7441 cgtcgaaaag acgctcggca tgaacgtcgc ctcgatcatc tgcctcaacg acctgctcgc
     7501 cttcatgaac ggcgacacgg acatggccaa ggccgtctcc tgccacaagg aagccgtggc
     7561 cgcctaccgt gcccgctacg gcgcctgaga ggcacgctga acagcgtcgg gcctgaggcc
     7621 cgacaacaaa aaaggaagcg tcaaatgacg cttccttttt ttatttgccg gtcaggcgaa
     7681 ctgatcaggc gccgagcttc ttgcgaagga gttcgttgac ctgggcggga ttggccttgc
     7741 cgcgcgtggc cttcatgcac tggcccacga gagcgttgaa ggccttctgc ttgcctgcgc
     7801 ggaactcgtc gacggacttc tggttcgccg caagcacttc atcaatgatg ccttcgagcg
     7861 cgccggtgtc tgaaatctgc ttcagaccct tgctctcgat gagcgcgtcg acgtcggtgc
     7921 cctcgccttc ccagacggcc gcaaagatct gcttggcgcc cttctggttg acggtgttgt
     7981 cgaggatgcg gccgagaatc gcggcgagga tttcaggcgt cacgggtgcc ttgccgaagg
     8041 tcatgccctc gacgccgttg acggcggcgg agagttcgcc catgacccag ttggcggcca
     8101 tcttgcggtc ggagaccttg gcggcaagcg caacgtaata gtcggcgatg tcgcggctgg
     8161 cggtcagcat cgaggcgtcg tactgcggca ggccgaattc ggactgcagg cgctcgcaga
     8221 gctggtgcgg aagctcgggc atctcgccgc gaaccctttc gatccattcg tcgctgattt
     8281 cgagcggaag aagatcggga tccgggaagt aacggtagtc catcgagtct tccttcgtgc
     8341 gcatctgacg cgtctcgccc ttgtccgggt catagaggcg ggtggcctga acgaccttgc
     8401 cgccgtcctc gatgagctcg atctggcgac ggacttcgta gtcgatggct tcctgcagga
     8461 agcggaagga attcaggttc ttgatttcac agcgcgtgcc gaactccttc tggccgacgg
     8521 ggcgcacgga cacgttggcg tcacagcgga agttaccctc ctgcatgttg ccgtccgaga
     8581 tgccgagcca gaccacgagg gcgtgaagcg cgcgggcgta gcccacagct tcggcggcgg
     8641 agcgcatttc cggttccgtc acgatctcga gaagcggcgt gccggcgcga ttgagatcga
     8701 tgccggagca gccctggacg aggccgtgaa tggacttgcc ggcgtcttcc tcaagatgag
     8761 ctcgcgtcag gttgacggtc ttgacgtacg gatccccctt cttgggctga acggggaagg
     8821 tcagcttgcc accgaggacg accggcaact cgaactggct gatctggtag cccttgggca
     8881 ggtcagggta gaagtagttc ttgcggtcga aaacggaatg attcgcgacc gtggcgtcga
     8941 tggcgaggcc gaagcggatg gccttctcaa cggcgctgtg gttgagcacc ggcaggctgc
     9001 cgggaagagc caggtcaacg tagcaggcgt tgacgttggg ctcgtcgccg aagtggcggg
     9061 cggcgccgga aaaaatcttg gaacgggtcg agagctgaac gtgcgtctca agaccgatca
     9121 caacttccca ctgcatggtt tatttctcct gagactctca ataacc
//
LOCUS       DBMDTI010000062         8876 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2183_length_8876_cov_2.130712, whole genome shotgun sequence.
ACCESSION   DBMDTI010000062 DBMDTI010000000
VERSION     DBMDTI010000062.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8876)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2183_length_8876_cov_2.130712"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            400..2214
                     /locus_tag="ACZPB6_04515"
     CDS             400..2214
                     /locus_tag="ACZPB6_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HIR1183770.1"
                     /translation="MKSFTLKLAAAAVAAACILPGTASASGGGSGPNVQYAVQGKIGE
                     VITNPYKIAPLTAVIRNGGYQLKDAAVRIVPKHGGAEIKYNVSDVQLRTHAGIPVFGL
                     YPDYNNTVEVSYTLVDGTKETRVENEVYRIYAPGIHTETNGTAAQHHAMFETEVKKVA
                     PEFKDRLYFINNFLPSGGNVARTTWNNPMGGALEWVYYPQNAVIDTAGDVRWYMFVSP
                     IYDPENIYKSGIMMGFHQADDGFLTFGYGQRYAKYDLMGREVFNRRLPAGYADFSHAM
                     DPAQNGHYFLRVSSADYRRPDGKRVHTVRDVILEVDQNGCAVDDFRLFDILDPYRDNV
                     IKAMDQGAVCLNVDASKSGQTLSAEELAKMDTNNQFGDIAGVGPGRNWAHVNSVDYDP
                     SDDSIIISSRHQSALIKIGRDKKVKWIVGSHEGWKKEFQDKLLTPIDKNGKPLKCEGS
                     KCEGGFDWTWTQHTGWRIDSKSDKDVFYLSVFDNGDARGMEQPALPEMKYSRAVIYRI
                     DQKKMTIEQVWEYGKERGFEWYSPITSVTEYQADKNSVFVYSATASLNMKQFKSVSPN
                     PVINEFKWGSTEPSVEIVLKSTMGYRALPISLDKAFSR"
     gene            2456..2803
                     /locus_tag="ACZPB6_04520"
     CDS             2456..2803
                     /locus_tag="ACZPB6_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183771.1"
                     /translation="MAFVFAAAALLALPCGAHACPDHAAVVQAVSASWQGEPASVGSH
                     ASRHEAAVRAECSAAAPEAPCTDRLCCVTPPAAPAPECRTAYSASVLPPATGSRPADT
                     SVILGRDSPPPKA"
     gene            2950..3416
                     /locus_tag="ACZPB6_04525"
                     /pseudo
     CDS             2950..3416
                     /locus_tag="ACZPB6_04525"
                     /inference="COORDINATES: protein motif:HMM:NF042810.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6803 family protein"
     gene            complement(3491..4963)
                     /locus_tag="ACZPB6_04530"
     CDS             complement(3491..4963)
                     /locus_tag="ACZPB6_04530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1183772.1"
                     /translation="MMKDTRGGVMTQRRRLLAGAGLAAAVPGRLLAAGAPDTLDPGAS
                     GVWDVIVAGSGAAGLSAAAAALESGAGHVLVLEKGPLVGGHSIYSSGSVSAVAPSRSH
                     DPNDTLGRFVEDALEVGGGTGDAAVLAKIGEDSAGVLDWLESLGVFWSEPFIAYSGKQ
                     AKSYAMPGNSAGRSYVLALMAHLRRFGCRLALSTPVTGFRPEGHAWVVEVKSPQGART
                     LRTRSLVIACGGFTANVEARMKINPLLTPDVPTSANPAGTVFDGATGELIECAGRNGA
                     FVTTGFGLQALPFWGGRLLDYQGADIYVDMHGRRFVNENSPWNVISNAILSLPERRCW
                     VITDDRSFKGATLGVKLINGVVRKSDSIDAMAAAMDIEPVVLARTIEDYNRAADKGYD
                     ARTGKRLFRQRIERPPFYWGAERIYVHTTLDGIRTNRAAEVIASSGGLVPGLYAAGEC
                     AGGIFGGDRLGGAGMTACFVMGREAGRQAAARAKELQAAA"
     gene            complement(4960..5610)
                     /locus_tag="ACZPB6_04535"
     CDS             complement(4960..5610)
                     /locus_tag="ACZPB6_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008541402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1183773.1"
                     /translation="MKRMIRTPLVRLVDDDDAFRESQREFLKAMGWQVEDYAGAADLL
                     ERDDLSRPGCLVLDIRMPGMTGVELQRRLFDAKCTLPVIFLTGHGDITTAVHTMKYGA
                     ADFLEKRGDPLALAGAVERACAKSMAAESDAAEADDYRRTFESLTQREREVFMLAAEG
                     ITNKDVAERLGIGAETVKMHKANAYAKLGVASALDAYRWLENLPADYRAGLAGTGK"
     gene            complement(5607..7592)
                     /locus_tag="ACZPB6_04540"
     CDS             complement(5607..7592)
                     /locus_tag="ACZPB6_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HIR1183774.1"
                     /translation="MNPLDVKRFSAAGFLAALFWTVLSGPVGAVVPGSSGAAPDGLSH
                     PSLTAPADDTRPVMTVAWPDTLVNPYDAFTIHETIAALKERLRGYCIRTATFAAAESA
                     EQLERLRPDFIFARSVFPLDAGVSSVRIATRRLKGLAEAERAVGAAFVVRSDSHYRTL
                     RDLKDRTVFSSLPTAIDGWLAAERVFRREGIDPTRLFARVVRRNNDYPDVVGAILSGR
                     ADMAVVPACLLETLGDEGLADVSGLRVLESEESKSAPVIPCRYSTELYPDISLLALPH
                     APSEMVRDMTIAILSLRNTAGAEWLTNVSHAGVDALLKELQLGPWAYLRDMSPAALYA
                     RHRTAFWLAAGLLLFLIFNELRLDRLVRRRTEDLRRSMAEQERLSAVAAATRTQLAMF
                     ERRSVVQQMSGMIAHEINAPVGAIRTWAALARIKCPTAAVADPAAAEALSNALSRIDG
                     EATRIADIVSRVRKYARREHERVAICELDAIVESAVRAYRAEERSDARVPINVAVAER
                     QAGVFGQPLELEVLVLNLVRNAASAVRRLDCLKGPEVAIGVELVRVADGRWRITVENP
                     GPVLSAQVFEKLNARAEGLACEVLDGEADREIPEGLGLGLTICRGIADSHGATLRFEA
                     RPEGGVRATVEIDAAGPAGEDTEGRKTMKVNEQGEDA"
     gene            7798..8721
                     /gene="aroD"
                     /locus_tag="ACZPB6_04545"
     CDS             7798..8721
                     /gene="aroD"
                     /locus_tag="ACZPB6_04545"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIR1183775.1"
                     /translation="MDRRSFITGLAAGTAALAHTGLAQASTSEAFSKKTRPVDTMNRI
                     GGWTNGSVEVIDIKGVRIGEGRPKLIAPTTSKTEADLIATVKKFADMPALNVIEVRID
                     YLSKIDPKDYARITRDAYAAAGDKVVLVTLRNGTDGGPFIADDEWYGRVYEAVLTEGR
                     ADIVDLELFRDRAMIDRLVKLARQQGVKVIISDHEFNFTPDEDEIIRRLMLEEQAGAD
                     ILKIAVMAHSPEDALAVMSATAKMRHYHSARPMLTMAMGRAGVLTRITGEGFGSDMTF
                     ASVGGKASAPGQIPAEDCLRVLETLHKAMNP"
BASE COUNT         1586 a         3113 c         2625 g         1552 t
ORIGIN      
        1 gactcgcggg atcgacggtc tcctcgacga agaggtcctc gaagcttgcg atcacggccg
       61 ccaccttcgg ctcgagcgcg acgccgtcgc ctgcggcatg ccgagctgcg agacggcccg
      121 ctgaaggttg tgcggcgcgc agagcgtcct gaagagaagc aaatggtcgc ggtcgagagg
      181 ttccctcggt tcttttctca tttttgaacg gttccattcg tcaaatatga cctgaacacg
      241 tctgaaattc acaatggttg gaaattccgg tcttctctaa ccttacagcc atggcgcgac
      301 agcctcccgg cagagaatgc cgcgacctaa agtacgtccg cgccagaaga caaaaccacg
      361 ctttcatctc cccgaccagg gatccaaagg agtttcctca tgaagtcctt cacactgaag
      421 ctcgccgccg cggccgttgc cgccgcctgc attcttccgg gcaccgcctc cgcctcgggc
      481 ggcggctccg gcccgaacgt ccagtacgcc gtccagggca agatcggcga agtcatcacg
      541 aacccgtaca agatcgcgcc gctcacggcc gtcatccgca acggcggcta ccagctcaag
      601 gatgccgcgg tccgcatcgt tcccaagcac ggcggcgccg aaatcaagta caacgtgagc
      661 gatgttcagc tgcgcaccca tgcgggcatc ccggtcttcg gcctctatcc cgactacaac
      721 aacacggtcg aggtgtctta cacgctcgtc gacggcacga aggaaacccg cgtcgaaaac
      781 gaggtctacc gcatctacgc gcccgggatc cacactgaga cgaacggcac ggccgcccag
      841 caccacgcca tgttcgaaac cgaagtgaag aaggttgcgc ccgaattcaa ggaccgcctc
      901 tacttcatca acaacttcct gccttcgggc ggcaacgtcg cacgcacgac atggaacaat
      961 ccgatgggcg gcgccctcga gtgggtctac tatccgcaga acgccgtgat cgacacggcg
     1021 ggcgacgtcc gctggtacat gttcgtctcg ccgatctacg atcccgagaa catctacaag
     1081 tccggcatca tgatgggctt ccatcaggcc gacgacggct tcctcacctt cggctacggc
     1141 cagcgctacg ccaagtacga cctgatgggc cgcgaggtct tcaaccgccg cctgcccgcc
     1201 ggctacgcgg acttctcgca cgccatggac ccggcccaga acggccacta cttcctgcgc
     1261 gtttcctcgg ccgactaccg ccgtcccgac ggcaagcgcg tccacacggt ccgcgacgtg
     1321 atccttgagg tcgaccagaa cggctgcgcg gtcgacgact tccgtctctt cgacatcctc
     1381 gacccgtatc gcgacaacgt catcaaggcg atggaccagg gcgccgtgtg cctcaacgtc
     1441 gacgcctcca agtccggcca gacgctctcc gccgaagaac tcgcgaagat ggatacgaac
     1501 aatcagttcg gcgacatcgc gggcgtcggt ccgggccgca actgggccca cgtcaactcc
     1561 gtcgactatg acccgtcaga cgactcgatc atcatctcct cccgccacca gtcggccctc
     1621 atcaagatcg gccgcgacaa gaaggtgaag tggatcgtcg gctcgcatga aggctggaag
     1681 aaggagttcc aggacaagct tctcacgccg atcgacaaga acggcaagcc gctcaagtgc
     1741 gaaggctcca agtgcgaggg cggcttcgac tggacttgga cgcagcacac cggctggcgc
     1801 atcgactcca agtccgacaa ggacgtcttc tacctctccg tcttcgacaa cggtgatgca
     1861 cgcggcatgg aacagccggc gctgcctgaa atgaagtact cccgcgccgt gatctacagg
     1921 atcgaccaga agaagatgac catcgagcag gtctgggaat acggcaagga acgcggcttc
     1981 gaatggtaca gcccgattac ctccgtgacc gaataccagg ccgacaagaa ctccgtcttc
     2041 gtctactcgg ccaccgcctc actcaacatg aagcagttta agagcgtgag cccgaacccc
     2101 gtcatcaacg agttcaagtg gggttccacg gaaccgtccg tcgaaatcgt gctcaagtcc
     2161 accatgggct accgcgcgct tccgatctcg ctcgacaagg ccttcagccg ataagcatcg
     2221 gcggattcag tcctgcggtc tctcttctct ttggaaccgc aggcctcctc aggaggcgca
     2281 tcgtcttcag gcgcctccct ccttctcgcg ccgcgccttc gggcacggcg ccttgttttt
     2341 atccgccgcc cgcaccgcct actgcgttcg ccctatgccg cccgaatgca tcggagctac
     2401 aatctattct gtcttcaatt ctttcagagg cggtttcaca atattgatcc gctcaatggc
     2461 cttcgtattc gctgcagccg cgctccttgc gctgccttgc ggtgcgcacg cctgtccgga
     2521 tcatgccgcc gtcgtgcagg cggtctccgc ctcatggcag ggagaacccg cctcagtcgg
     2581 gtcacacgcc tcgcgtcacg aggcggccgt tcgggccgaa tgttccgcag cggcgcccga
     2641 agcgccctgc accgatcgcc tctgctgcgt gacgccgccc gcggcgcccg cgcccgagtg
     2701 tcggactgcg tactccgcct ccgtcctccc gcccgccaca ggttcccgtc ccgccgacac
     2761 gagcgtcatc ctcggcaggg actctccgcc tccgaaagcc tgaagcttcg ggccctgcgc
     2821 ccgcacgcct tccggacgga cagccgtccg tccgcgtcac tcaagcttct tttttcggag
     2881 acacacaaaa tggaaatgac ccactatatg gagctcatga tgggctcctg gcacatgctc
     2941 gtcctcttca tggccgcacc gatgctgctc gcggagtgct acgtcgtgag cgaaatcctt
     3001 ctgctcatca agccgcctca gggcggcgcc ctcgccgcct tcaacaaggc ctcggcctat
     3061 gcagccgcgc tcggcatcgc gctcctctcg ggctgcgtgg cccttcagtt ctcgtcggtc
     3121 gtcgcatggc gcacctgggt cgacgccgtt tcggccctcg cctacgtggc cgcgggcctt
     3181 ccgttcatct acgtcggcct tctcgaggcg gcctcgtcgc ccgcagcagg acggccgtct
     3241 ggaagggacg ctcggccgtc gcggcggtgg tcgtctatct cgtcctctcc cacgccgcca
     3301 tggtcttcgg gatgttcgat cccgtgcagt tcggctggcg ggcttcagac gacatgcaca
     3361 tgacgcacgg cgctcacgcg atgcctgaga cgcacgcagg ccacgtcatt cactgacgca
     3421 ggccgcaaaa aggcgcggcg cgaagcctgc gggcttctct gccgtgccct gcgtccatgc
     3481 gcgcggatgc tcacgctgcg gcctggagct ccttcgctct tgccgcagcc tgtcttccag
     3541 cctcgcgtcc catcacgaag caggcggtca tgcctgcgcc gccgagtcgg tcgccgccga
     3601 aaatgccgcc cgcgcattcg ccggcggcgt agagtccggg cacgaggccg cccgaggacg
     3661 caatgacttc ggccgcacgg ttcgtcctga tgccgtcgag cgtcgtgtgc acgtagatgc
     3721 gctcggctcc ccagtagaag ggcggccgct cgatcctctg gcgaaagagc ctcttgcccg
     3781 tgcgtgcgtc gtaccccttg tccgccgccc tgttgtagtc ctcgatcgtc ctcgcgagca
     3841 cgacgggctc gatgtccatg gcggccgcca tcgcgtcgat cgagtccgac ttcctcacga
     3901 cgccgttgat gagcttgacg ccgagcgtcg ctcccttgaa ggaacggtcg tccgtgatca
     3961 cccagcagcg cctttcggga agcgacagaa tggcgttcga aatcacgttc cagggactgt
     4021 tctcgttgac gaatcgcctg ccgtgcatgt cgacgtagat gtcggcgccc tgatagtcga
     4081 gcaggcgtcc gccccagaac ggaagcgcct gaaggccgaa gcccgtcgtc acgaacgcgc
     4141 cgtttcgtcc cgcgcactcg atgagttcgc ccgttgcccc gtcaaagacc gtgcccgccg
     4201 gattggcgct cgtcggaacg tcgggcgtga gcaggggatt gatcttcatg cgcgcctcga
     4261 cgttggccgt gaagcccccg caggcgatga caaggcttcg cgtcctgagc gttcttgcgc
     4321 cctgcggcga tttcacttcg acaacccacg catgcccctc gggtctgaag cccgtcacgg
     4381 gcgtcgagag cgcaagcctg cagccgaagc gcctcaggtg cgccatgagc gcaagcacgt
     4441 agctgcggcc cgccgagttg cccggcatcg cgtagctctt ggcctgcttg cccgaatacg
     4501 cgatgaacgg ttcgctccag aacacgccga ggctctcgag ccagtcgagc acgcccgccg
     4561 aatcctcgcc gatcttcgcg agcaccgccg cgtctcccgt gccgccgccg acctcgagcg
     4621 catcctcgac gaacctgccg agcgtgtcgt tcggatcgtg cgagcgcgag ggcgccacgg
     4681 ccgaaaccga accgctcgaa tagatcgaat gtccgcccac gagcggcccc ttttcaagaa
     4741 ccagcacgtg gcccgcgccc gactccagag ctgcggccgc agccgagagc cccgccgcac
     4801 ccgagcccgc gacgatcacg tcccagacgc ccgaggcgcc cggatcgagc gtgtcgggcg
     4861 cgcccgcggc aagaagccgg cccggcaccg ccgccgcaag acccgcgccc gccagaagac
     4921 ggcgcctctg cgtcatgacg ccgcctctcg tgtccttcat catttgcccg tccccgcaag
     4981 gcccgccctg tagtccgcag gcaggttctc gagccagcgg taggcgtcga gcgcgcttgc
     5041 gacgccgagc ttggcgtagg cgttggcctt gtgcatcttc accgtctcgg cgccgatgcc
     5101 gagcctttcg gcaacgtcct tgttggtgat cccctcggcg gcaagcatga acacctcgcg
     5161 ctccctctgt gtaagcgact cgaaggtcct cctgtagtcg tccgcctcgg ccgcatcgct
     5221 ctcggcagcc atcgacttcg cgcaggcgcg ctcgacggcg cccgcaagcg caagcggatc
     5281 gccgcgcttt tcaagaaagt cggctgcgcc gtacttcatc gtgtgcacgg ccgtcgtgat
     5341 gtcgccgtgc cccgtgagga agatgacggg cagcgtgcat tttgcgtcaa agagcctgcg
     5401 ctgaagctcc acgcccgtca tgccgggcat gcggatgtcg agcacgaggc agcccggcct
     5461 cgagaggtcg tcgcgctcga gaaggtcggc cgcacccgca tagtcctcga cctgccagcc
     5521 catggccttc agaaactcgc gctggctctc gcgaaacgca tcatcgtcgt cgacaagcct
     5581 cacgagcggc gtcctgatca tccgcttcat gcatcttctc cctgttcatt caccttcatg
     5641 gttttcctgc cttcggtgtc ctctcctgcg gggcctgccg cgtcgatctc gaccgtggcc
     5701 cgcacgccgc cctcgggtct tgcctcgaag cgcagcgtcg caccgtgact gtcggcaata
     5761 cccctgcaga tcgtgagtcc gagtccaagg ccttccggaa tctcacggtc ggcctcgccg
     5821 tcgagcacct cgcaggcaag accttcggcg cgggcgttga gtttttcgaa gacctgcgcc
     5881 gagaggacgg gccccggatt ctcgaccgtg atgcgccacc tgccgtccgc gaccctcacg
     5941 agctcgaccc cgatggcaac ctcggggccc ttgaggcagt cgaggcgcct cacggcggag
     6001 gccgcattgc gaacgaggtt gaggacgagc acctcgagtt cgagcggctg tccgaagacg
     6061 cccgcctggc gctcggcgac tgcaacgttg atcgggacgc gggcgtcgct tcgctcttcg
     6121 gccctgtagg ccctgacggc gctctcaacg atggcgtcga gttcgcagat cgcgacgcgc
     6181 tcgtgctcgc gccttgcata ctttcggacc ctcgagacga tgtcggcgat cctcgtcgcc
     6241 tcgccgtcga tgcgcgagag tgcattggag agcgcctcgg ccgccgccgg atcggccacc
     6301 gccgccgtcg ggcacttgat tcttgcaagg gcggcccaag tgcggatcgc gcctacgggc
     6361 gcattgatct catgggcgat catgcccgac atctgctgca cgacgcttct gcgctcgaac
     6421 atcgcgagct gggttcgcgt ggcagccgcg accgccgaaa gcctctcctg ctcggccatc
     6481 gagcgcctga ggtcctcggt cctgcgcctc acgagcctgt cgagcctcag ctcgttgaag
     6541 atgagaaaca gaagaagccc cgccgccagc cagaaggcgg ttctgtgcct tgcatagagc
     6601 gcggcgggac tcatgtccct cagataggcc caggggccga gctgcagctc cttgagaagc
     6661 gcgtcgacgc ctgcgtggga aacgttggtg agccattcgg cgcccgcagt gttcctgagg
     6721 ctcagaatgg cgatcgtcat gtcgcgcacc atttcggacg gcgcgtgcgg cagtgccaga
     6781 agactgatgt cgggatagag ctccgtcgag tacctgcacg gaatgacggg cgcgctcttc
     6841 gactcctcgc tctcgagcac cctcaggcct gagacgtccg cgaggccctc gtcgccgagc
     6901 gtctcgagca gacatgccgg cacgaccgcc atgtcggccc tccctgagag aatggcgccc
     6961 acgacgtcgg gatagtcgtt gttgcggcgc acgactctgg caaacagccg cgtgggatcg
     7021 atgccctcgc gcctgaagac gcgctcggcg gcaagccaac cgtcgatggc cgtgggcaga
     7081 gacgaaaaga ccgtgcggtc cttgaggtca cggagcgtcc tgtagtgcga atcgctcctc
     7141 acgacgaagg ccgcgcccac ggcgcgctcg gcctcagcga gccccttgag gcgcctcgtg
     7201 gcgatgcgca cagaggagac gcccgcgtcg agcggaaaca ccgaacgcgc aaagatgaag
     7261 tcgggcctga gcctctcgag ctgctccgca ctctcggcgg ccgcgaaggt cgccgtcctg
     7321 atgcagtagc cgcgcagcct ctccttgagg gcggcgatgg tctcgtggat ggtaaaggcg
     7381 tcgtacggat tgacgagcgt gtcgggccag gccaccgtca tcacggggcg ggtgtcgtcg
     7441 gcaggcgccg tcagggacgg gtgcgaaagc ccgtccggag ccgctccgga agatcccggc
     7501 acgactgcgc cgaccgggcc cgaaagcacg gtccagaaca gcgccgcaag aaaacccgca
     7561 gccgaaaaac gcttgacgtc gagaggattc atgatgcttc cgagaggagg aagaacccta
     7621 gggtttacac ttacccccca tgcaggggta ggcgatttaa aaaaactcta gcagaatttg
     7681 gacctgaagc agaacttcat tttcaagtcc gccgaagagc ggaccgctcc tcggaaaaac
     7741 agttcgaccc aaactttcag cattccaacg cgaacctgca acacaaggac atccacaatg
     7801 gaccgccgct ccttcatcac gggccttgcc gccggcaccg ccgctcttgc gcacacgggc
     7861 cttgctcagg ccagcacctc cgaagccttc tccaagaaga cgcgtcccgt cgacaccatg
     7921 aaccgcatcg gcggctggac gaacggcagc gtcgaagtga tcgacatcaa gggcgtgcgc
     7981 atcggcgaag gtcgtccgaa gctcattgca ccgacgacct ccaagaccga agccgacctg
     8041 attgccacgg tgaagaagtt tgcggacatg cccgcgctca acgtgatcga ggtccgcatc
     8101 gactacctca gcaagattga tccgaaggac tacgcccgca tcacccgcga cgcctatgcc
     8161 gccgcaggcg acaaggtcgt gctcgtcacg ctgcgcaacg gcacggacgg cggtcccttc
     8221 atcgccgacg acgaatggta cggccgcgtc tacgaggccg tcctcacgga aggccgcgcc
     8281 gacatcgtcg accttgagct cttccgtgac cgcgccatga tcgaccgtct cgtcaagctt
     8341 gcccgccagc agggcgtcaa ggtcatcatc tccgatcatg aattcaactt cacgcccgac
     8401 gaggatgaaa tcatccgccg cctgatgctc gaagaacagg ccggcgccga catcctcaag
     8461 atcgccgtca tggcccacag ccccgaggac gccctcgcgg tgatgagcgc cacggccaag
     8521 atgcgccact accactccgc ccgtccgatg ctcacgatgg ccatgggccg tgcaggcgtc
     8581 ctcacccgca tcacgggcga gggcttcggc agcgacatga cgttcgcgag cgtgggcggc
     8641 aaggcctcgg ctccgggcca gatcccggcc gaggactgcc tcagggttct tgaaacgctt
     8701 cacaaggcca tgaacccgta aggctcctct tcctcctgcg ggcctgcgcc cctccatccc
     8761 ccgggggcgc gggcctgtct ccttcgatcc gaaggacaaa cgttctcctt tttcatcaac
     8821 ctacgactca tcacaagttc aggaatttat tatgaagaag actctgatcg cctttg
//
LOCUS       DBMDTI010000063         8800 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2197_length_8800_cov_3.790436, whole genome shotgun sequence.
ACCESSION   DBMDTI010000063 DBMDTI010000000
VERSION     DBMDTI010000063.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8800)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2197_length_8800_cov_3.790436"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="ACZPB6_04550"
     CDS             <1..390
                     /locus_tag="ACZPB6_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014385.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR1183776.1"
                     /translation="ALSMPVLNWGSLAAQEERAAASLDKAKANYLSVIVKALEETDNA
                     LNCIRSAQDRTALLRTALQHTEKTYELTGLEYRSGIGDYSMLLEAQQRLLTLRESVTN
                     NMTELSLQHVKLYRALGGAWKAQESAR"
     gene            387..1658
                     /locus_tag="ACZPB6_04555"
     CDS             387..1658
                     /locus_tag="ACZPB6_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1183777.1"
                     /translation="MTEQENNDRTKLLLKDESRSRYVLWAAGGVLAALVAGAWYWYQG
                     TQNTNAVKYVTEAVERGDVHVSVSADGTLAPVRTVSLGSELSGIVREVNVDVNDEIKE
                     GQSLIVLDKRNLEAKVLQNRASLRSAEASRRQSAASLREAELKLKRMQELNRLSKGTM
                     PSKTELETQAASVDTARAAVAVADANIADAMASLQTAENDLSKADIKSPIDGVVLARS
                     VEPGYAVAASLQAVELLTLATDLSQLELEVSVDEADIGVVKQGQKAYFTVSAYPNRRF
                     PAELTKVSYGATTTENVVTYTAYLQVDNQQMQLRPGMTASATISTADKQDVLLVPNSA
                     FRFRPKAASESDKPKMNAMMPGPPHGAASKKVKEIAQHGQASRTVYVLRDGKPVRVDV
                     VTGLTDGMQTEIVSGDIAEGDKVVVDQQKGA"
     gene            1668..2369
                     /locus_tag="ACZPB6_04560"
     CDS             1668..2369
                     /locus_tag="ACZPB6_04560"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1183778.1"
                     /translation="MGQPLVELVDIRKRYGTGEAAFEALRGVSFSIDEGEFVAIMGAS
                     GSGKSTAMNIIGALDSPTAGAYLFSGMHVESLSRDQRALLRRKYLGFVFQGFNLLART
                     TALENVELPLLYRGMGASERRKLARAALDRVGLLDWEHHTPAELSGGQQQRVAIARAI
                     VTRPKLLLADEPTGSLDTARSEEIMALLSALNAEDGITVVLVTHEPDMARYAKRHLVF
                     RDGLLISDSKTEAAS"
     gene            2366..3574
                     /locus_tag="ACZPB6_04565"
     CDS             2366..3574
                     /locus_tag="ACZPB6_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1183779.1"
                     /translation="MIGNAFVLAVRQISRNPLRSLLTVLGVVIGVMAVITMVTVGNGA
                     SQAIRTQIESFGNNQLMLRPGLRMGPGQSVGAPNFTLEDADALKRQIAGIVAVAPQAS
                     KTMTVVANGRNWQTSVIGTDNNYFTIDNREIVDGRTFEQGEMQSGAAVCVIGGTIQKE
                     LFGEGAEVIGRTIRTGSFSCRVVGLLKTKGTAAMGGDQDDLLVMPIKTLQRRILGKNR
                     VGALLVSVNPQSDRDRLRDAVTELMRERRSLSDGDDDNFQILDTAEIAAKVASTTQIM
                     TTLLAAVAAVSLLVGGIGIMNIMLVSVTERTREIGIRLAIGALEREVLLQFLIEALML
                     GCMGGALGVLAALGVSKALAVYMGVPFIFSPGINLLAFTVSALTGVVFGFFPARRAAK
                     LDPIEAVRHE"
     gene            complement(3683..4171)
                     /locus_tag="ACZPB6_04570"
     CDS             complement(3683..4171)
                     /locus_tag="ACZPB6_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1183780.1"
                     /translation="MASINKVILIGNLGRDPETRYTTDGGTAITTLNIATTRRYRNAE
                     GQTVSETEWHRVVLFSRLAEIAKEYLHKGSQCYIEGRLRTRKYQAKDGTDRYATEIIG
                     ETMQLLDRRQSDNQGMDDGFESAPRASRPAPQPAPQPARQSAPVTSNTPFDQLEGDDV
                     PF"
     gene            complement(4197..5399)
                     /locus_tag="ACZPB6_04575"
     CDS             complement(4197..5399)
                     /locus_tag="ACZPB6_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1183781.1"
                     /translation="MTAQEKRSSISLAGIFALRMLGLFLILPVFAVYARHLPGGDSDF
                     LVGLTLGIYGLTQSVLQIPFGAASDRLGRKPVIVAGLLLFIAGSVVAAMGTTIWAVMI
                     GRALQGAGAISAAVTALIADSVRDHVITKAMALVGASIGLTFAFSLVAAPPLTELWGV
                     PGLFWLTAVLSAGAVWIVLKVVPDVPAVATEEADDHQPWPKVAFDPQLMRLNAGIFCL
                     HCVQMALFVVIPTQLVEMGLPVLHHWYIYLPAVLIGFAVMMKPIIWAERNRKVASLLR
                     MNVLLLAVVFVLFSFLMHSVWEVAFLVTLFFIGFNILEATLPGLISRTAPKADKGLAL
                     GIYNTTQSFGLFVGGAAGGWIAQHFSSQAVFYASSFAMLCWFLLALGLKEPKPRRQHE
                     QGEAIQVR"
     gene            5543..8329
                     /gene="uvrA"
                     /locus_tag="ACZPB6_04580"
     CDS             5543..8329
                     /gene="uvrA"
                     /locus_tag="ACZPB6_04580"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1183782.1"
                     /translation="MKAISVRGARTHNLKNVSLDIPRDKLTVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESISAYARQFLELRPRPDVDGIDGLSPSIAIGQRRSSGSSRSVVGTVT
                     EISDYMRLLFARAGVPYCPEHGKHLEMSSIASIVDRTLTLSDGAKIMVLAPVSRGKTG
                     NFKAYFEKSLADGYVRFRIDGVVQVLEVAPSLNDGRPHDVDVVVDRLKVSPEARTRLA
                     ESCQSASDLSNGRVYIEEFGTGMHYEFSTQFACPLCSFSVPALEPIHFSPFNPAGCCT
                     ACEGTGTVRAFDAGKVVTAETGSIEAGAIPGWDARNGVKFKLISAAAEVLGVSVRTPW
                     CEYPEEVKQVFLYGNEQTRKLRVPFIGVMNDLQRQWDDPRTLSYMRQGLETYRSDVTC
                     PVCKGERLRPELLSVYVGDGDKRYSYGEMNSMSLSQLRAAFAGLEFSERRAAVAERLT
                     AAISSRLAFLENVGLGYLSLNRRADTLSGGEMQRIRLASQIGSGLSGVLYVLDEPSIG
                     LHQRDNERLLKSLADLRDLGNTVIVVEHDEDAMRSADYLVDMGPGAGEKGGEVLAAGT
                     PEEVMASERSLTGKYLRGELKIPVPKERLKPERGWLTLSGACGHNLRNVTLRVPVGLL
                     SVVTGVSGSGKSSLVIDTLYAAMSRTINRATTMRPLPYGRIENIEAFDKVIMVDQSPI
                     GRTPRSNPATYTGIFSAIREVFEQTQTARERGYAASRFSFNVKGGRCEACQGDGIVCM
                     QMQFLPDVYVPCDVCHGRRYNRETLEVTYQGRNIADVLEMTVDEALPFFDAYPKIKRV
                     LQALADVGLGYIKLGQSATTFSGGEAQRIMLAAELSRPETGRTLYILDEPTTGLHFKD
                     IEQLLGVLRRLTDQGNTVLLIEHNLDVVKCADYVVDMGPDGGENGGVIMAEGTPEEVA
                     ESDSVSSPYLKRVL"
     gene            8361..>8800
                     /locus_tag="ACZPB6_04585"
     CDS             8361..>8800
                     /locus_tag="ACZPB6_04585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016228.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009226 - nucleotide-sugar biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1183783.1"
                     /translation="MILGASGRLGRALWESRPQTLEVTALTHAELDVTDIRAVEAVIA
                     LARPDVVINAAAWTDVAGAQTNAAAARAVNAVAPGAMGRLFARTGVRIVHFSTDYVFS
                     GEGSSPWNEASEAHPRQAGVYGVTKHEGERLLEESGVSGAIVRAG"
BASE COUNT         1631 a         2719 c         2956 g         1494 t
ORIGIN      
        1 gcgctgtcca tgcccgtgct caactggggc agtctggccg cacaggagga gagggccgcc
       61 gcatcgctgg acaaggcgaa ggcgaactac ctatccgtca ttgtgaaggc gcttgaggag
      121 acggacaacg ccctcaactg catccggtcc gcacaggacc gaacggcgct tctcaggacg
      181 gcccttcagc acacggagaa gacctacgag ctcacggggc tcgaatacag gtcgggcatc
      241 ggcgactatt cgatgcttct tgaagcacag cagcgcctcc tcaccctgag ggagagcgtg
      301 acgaacaaca tgacggagct ttcccttcag cacgtgaagc tctaccgcgc cctgggcggc
      361 gcctggaagg cacaggagag tgcaagatga ccgaacaaga aaacaacgac cgcacgaagc
      421 ttctgctcaa ggatgaaagc cgctcgcgct atgtcctctg ggcggcgggc ggagttctgg
      481 ccgcgctcgt ggcaggcgcc tggtactggt atcagggcac gcagaacacc aacgccgtca
      541 agtacgtgac cgaggccgtc gagcgcgggg acgtgcacgt cagcgtgtcc gcggacggca
      601 cgctcgcacc tgtgcgcacc gtctcgctcg gttccgagct ttcgggcatc gtgcgcgagg
      661 taaacgtcga cgtcaacgac gagatcaagg agggccagag cctcatcgtg cttgacaagc
      721 gcaatctgga ggcgaaggtg cttcagaaca gggcttcgct gcgaagcgcg gaggccagcc
      781 gcaggcagag tgcggcgagc ctgagggagg cggagctcaa gctcaagcgc atgcaggagc
      841 tcaatcgtct ctccaagggc accatgccga gcaagaccga gcttgagacg caggccgcgt
      901 ccgtcgacac ggcgcgcgcg gcggtcgccg tggccgatgc gaacatcgcc gacgccatgg
      961 cctcgctcca gacggctgag aacgatctct ccaaggccga catcaagtct cccatcgacg
     1021 gcgtggtgct cgcccgttcg gtcgagcccg gctatgcggt ggcggcaagc ctgcaggcgg
     1081 tcgagctgct gacgctcgcc accgatctga gtcagcttga gctcgaggtg tccgtcgacg
     1141 aggccgacat cggcgtcgtc aagcaggggc agaaggccta ctttacggtg agcgcctatc
     1201 cgaaccgccg cttccccgct gaactgacca aggtttccta cggcgccacg acgacggaga
     1261 acgtggtgac ctacacggcc tatcttcagg tcgacaacca gcagatgcag ctgcgccccg
     1321 gcatgacggc ttcggccacg atctcgacgg ctgacaagca ggacgtgctc cttgtcccca
     1381 attccgcctt ccgcttccgt cccaaggccg catcggagag cgacaagccg aagatgaatg
     1441 ccatgatgcc ggggccgccc cacggcgccg ccagcaagaa ggtcaaggag attgcccagc
     1501 acggccaggc gagcaggacc gtttacgtgc tgcgtgacgg caagcccgtg cgcgtcgacg
     1561 tcgtcacggg tctcaccgac ggcatgcaga ccgaaatcgt ctcgggcgac atcgccgagg
     1621 gcgacaaggt cgtcgtcgac cagcagaagg gcgcctgagg agacgcaatg ggacagccac
     1681 tagttgaact cgtcgacatc cgcaagcgct acggcacggg cgaggcggcc ttcgaggccc
     1741 tgcgcggcgt gagcttttcg attgacgagg gcgaattcgt cgccatcatg ggcgcgtcgg
     1801 gctcgggcaa gtcgacggcc atgaacatca tcggcgcgct cgacagcccg acggcgggcg
     1861 cgtatctctt cagcggaatg cacgtcgaga gcctgtcgcg cgaccagcgt gcgctcctgc
     1921 ggcgcaagta cctcggcttc gtctttcagg gcttcaatct tctcgccaga acgacggcgc
     1981 ttgagaacgt ggagcttccg ctcctctacc gcggcatggg cgcctccgag cgccgcaagc
     2041 tggcgcgggc cgcgctcgac cgcgtaggcc tgctggactg ggagcaccat acgccggcgg
     2101 agctctcggg cggacagcag cagcgcgtcg cgattgcgcg cgccatcgtc acgcgcccga
     2161 agctgcttct cgcagacgag ccgacgggca gtcttgacac ggcgcgaagc gaggagatca
     2221 tggcgcttct ttcggccctc aacgccgagg acggcatcac ggtcgtgctc gtcacgcacg
     2281 agcccgacat ggcccgctac gcaaagcgcc accttgtctt cagggacggc ctgctcatca
     2341 gcgacagcaa gacggaggcc gcgtcatgat cgggaatgca ttcgtgctgg ccgtgcgcca
     2401 gatcagtcga aatccgctca gaagcctgct caccgtgctc ggcgtcgtga tcggcgtcat
     2461 ggccgtgatc acgatggtga cggtcggcaa cggggcctcg caggccattc gcacgcagat
     2521 cgagtccttc ggcaacaacc agctgatgct gcgtcccggt cttcgcatgg ggccgggcca
     2581 gagcgtgggc gcgccgaact tcacgctcga ggacgccgat gcgctcaagc gccagatcgc
     2641 gggcatcgtc gccgtggcgc ctcaggcctc caagaccatg acggtcgtgg ccaacgggcg
     2701 caactggcag acgagcgtga tcggcacgga caacaactac tttacgatcg acaaccgcga
     2761 aatcgtcgac ggacgcacct tcgagcaggg cgaaatgcag tcgggtgccg ccgtctgcgt
     2821 gatcggcggc accattcaga aggagctctt cggcgaagga gcggaggtca tcggacgcac
     2881 catccgcacg ggaagcttct cgtgcagggt cgtgggcctt ctcaagacca agggtacggc
     2941 cgccatgggc ggcgatcagg acgacctgct cgtcatgccg atcaagacgc tccaacgccg
     3001 catcctcggc aagaaccgag tcggcgccct tctggtttcg gtgaatccgc agtcggaccg
     3061 agaccgcctg cgcgatgccg tcacggagct catgcgcgag aggcgcagcc tctccgacgg
     3121 cgatgacgac aacttccaga ttctggacac ggcggagatc gccgccaagg tggcatccac
     3181 cacgcagatc atgactacgc tgctcgccgc agtggcggcc gtgagcctgc tcgtgggcgg
     3241 catcggcatc atgaacatca tgctggtgtc cgtgacagag cgaacccgtg aaatcggcat
     3301 ccgactcgcc atcggcgccc tcgagcgcga ggtgctgctc cagttcctga tcgaggcgct
     3361 gatgctcggc tgcatgggtg gcgcgctcgg cgtgctggcg gctctgggcg tctcgaaggc
     3421 ccttgcggtc tacatgggcg tgcccttcat cttcagcccg ggcatcaatc tgctcgcctt
     3481 caccgtgtcg gccctcacgg gcgtggtgtt cggcttcttc cccgccagac gtgcggccaa
     3541 gctcgacccg atcgaggcgg tgcgtcacga gtgacgcggg agcgcagcgg aaaagcagaa
     3601 aggtccgaag cgtgagcttc ggacctttca ttttgtttgc tggtgcgctt gccagattcg
     3661 catgaaggcg aaagcgcacg gcttagaacg gaacgtcgtc gccttcaagc tggtcgaagg
     3721 gggtgttgga cgtcaccggg gcgctctggc gggctggctg gggagctggc tggggagcgg
     3781 gacgcgaggc gcgcggagcg ctttcgaagc cgtcgtccat gccctggttg tcggactggc
     3841 ggcggtcgag gagctgcatc gtttcgccga tgatttcggt ggcgtagcgg tcagtgccgt
     3901 ccttggcctg gtacttgcgg gtgcgcaggc ggccttcgat gtagcactgg ctgcccttgt
     3961 ggaggtattc cttggcgatt tcggcaaggc gggagaagag gaccacgcgg tgccattcgg
     4021 tttcgctgac ggtctggcct tcggcgttgc ggtagcggcg cgtcgtggcg atgttgagcg
     4081 tcgtgatggc ggtgccgccg tccgtcgtgt agcgggtttc gggatctcgg ccgagattgc
     4141 cgatcaggat taccttgttg atggaagcca tgattaacta acttgcaaaa ttatatctat
     4201 cgaacttgaa ttgcttcacc ttgttcatgc tgtctgcggg gcttgggctc cttgaggcca
     4261 agtgcaagca ggaaccagca aagcatagca aaactcgatg catagaacac ggcctgcgat
     4321 gaaaaatgtt gggcgatcca gccgcccgcg gcgccgccga cgaagagccc gaagctctgc
     4381 gtcgtgttgt agatgccgag tgcaagcccc ttgtcggcct tgggggccgt gcgcgagatg
     4441 aggccgggca gcgtggcctc gaggatgttg aagccgatga agaagagcgt cacgaggaag
     4501 gccacctccc agacggagtg catcaggaag gagaagagca cgaagacgac cgcgagaagg
     4561 agcacgttca tgcgcaggag cgaggcgacc ttgcggttgc gctcggccca gatgatgggc
     4621 ttcatcatga cggcgaagcc gatgagcacg gcgggaagat agatgtacca gtggtgcagg
     4681 acgggaagtc ccatctcgac gagctgcgtc ggaatgacga cgaagagggc catctgcacg
     4741 cagtgcaggc agaagatgcc cgcgttgagg cgcatgagct gagggtcgaa ggcgaccttg
     4801 ggccagggct ggtggtcgtc cgcctcctcg gtggcgacgg cgggcacgtc gggcacgacc
     4861 ttgaggacga tccagacggc gcctgcggaa aggacggccg tgagccagaa gagtccgggc
     4921 acgccccaga gttcggtgag cggcggggcg gcgacgaggc tgaacgcgaa ggtgaggccg
     4981 atcgaggcgc cgacgagcgc catggccttc gtgatgacgt ggtcgcgcac gctgtccgca
     5041 atgagcgcgg tgacggcggc ggagatggcg cccgcgccct gaagcgcgcg gccgatcatg
     5101 acggcccaga tcgtggtgcc catggcggcg acgacgctgc ctgcaatgaa gagcaggagg
     5161 ccggcgacga tgacgggctt gcggccgaga cggtcgctcg cagcgccgaa cggaatctgc
     5221 aggacgctct gggtgaggcc gtagatgccg agcgtgaggc cgacgagaaa gtcgctgtct
     5281 ccgccgggca ggtgcctggc atagactgcg aagacgggaa gaatgaggaa gagtccgagc
     5341 atgcgcaggg cgaaaatgcc tgcgagggag atgctggaac gcttctcctg agccgtcatg
     5401 gctccggagg cgtttgaggg atcgtgtttg gcgcgcatga gtagccgtaa gaatgaaaag
     5461 tcgggaggac atcctttccc cgaacggtag gacagtctat atttgttagt ttgattttgc
     5521 ccttaagagc cgtcttcaca tgatgaaagc catttccgtg cgtggcgcgc gcacccacaa
     5581 cctcaagaac gtcagtctgg atatcccgcg agacaagctc acggtcatca cgggactttc
     5641 gggcagcggc aagagttcgc tcgccttcga cacgctttac gcggaggggc agcgccgcta
     5701 cgtggagagc atttcggctt atgcgcggca gttccttgag ctgcgtccga ggcccgacgt
     5761 cgacggcatc gatggacttt cgccctccat cgccatcgga cagcgcagga gttcgggctc
     5821 gtcgcgctcg gtcgtgggca ccgtcaccga aatctcggac tacatgaggc ttctcttcgc
     5881 ccgcgcgggc gtgccctact gccccgagca cggcaagcat ctggagatga gctcgatcgc
     5941 ctcgatcgtc gaccgcacgc tcacgctctc ggacggcgcg aagatcatgg tgctcgcgcc
     6001 tgtttctcgc ggcaagacgg gcaacttcaa ggcgtatttc gagaagtccc tcgccgatgg
     6061 ctacgtgagg ttcaggatcg acggcgtcgt tcaggttctc gaggttgcac cgtccctcaa
     6121 cgacggccgg ccgcacgacg tggacgtcgt ggtcgaccgt ctcaaggtct cgcccgaggc
     6181 ccgcactcgt ctggctgaaa gctgccagag cgcatccgac ctgagcaacg gccgcgtcta
     6241 catcgaggag ttcggcacgg ggatgcacta cgagttcagc acgcagttcg cctgtccgct
     6301 ctgcagcttc agcgttcctg cgctcgaacc cattcacttc tctcccttca atcccgccgg
     6361 atgctgcacg gcctgcgaag gcacgggcac cgtgcgggcc ttcgacgcag ggaaggtcgt
     6421 aacggccgag acgggatcga ttgaggcggg cgccatcccg ggatgggatg cgaggaacgg
     6481 cgtcaagttc aagctcatct ccgctgcggc ggaggtgctc ggcgtgtccg tccgcacgcc
     6541 ctggtgcgag tatccggaag aggtgaagca ggtcttcctc tatggcaacg agcagacgcg
     6601 caagctacgg gtgcccttca tcggcgtgat gaacgacctg cagaggcagt gggacgatcc
     6661 gaggaccctc agctacatga ggcagggtct cgagacgtac cgctccgacg tcacttgtcc
     6721 cgtctgcaag ggcgaacgac ttcgccccga gcttctgtcc gtctacgtgg gggacggcga
     6781 caagcgctac tcctacggcg agatgaactc gatgagcctc tcgcagcttc gagcggcgtt
     6841 cgcgggactt gagttctcgg agcgcagggc tgcggtggcc gagcgtctga cggcggccat
     6901 ctcgagccgc ctcgccttcc ttgagaacgt gggtctgggc tatctctccc tgaaccgccg
     6961 cgccgacacg ctgtcgggcg gcgagatgca gcgcatcagg cttgcgagcc agatcggctc
     7021 ggggctctcg ggcgtgcttt acgttctgga cgagccgagc atcggcctgc atcagcgtga
     7081 caacgaaagg cttttgaaga gcctcgccga cctgagggac ctcggcaaca ccgtcatcgt
     7141 cgtcgagcat gacgaggacg ccatgcgttc ggccgactat ctcgtcgaca tggggcccgg
     7201 tgcgggcgag aagggcggcg aggttctcgc tgccggcacg cccgaggagg tcatggcaag
     7261 cgagcgctcc ctgacgggca aatacctcag gggcgagctg aagattcccg tgccgaagga
     7321 gcgcctcaag cccgagcgcg gctggcttac gctttcgggc gcctgcggcc acaacctgcg
     7381 caacgtcacc ctcagggttc ccgtcggact tctgagcgtc gtgacaggcg tttcgggctc
     7441 gggcaagtcc tcgctcgtca tcgacacgct ctatgcggcg atgtcccgca cgatcaaccg
     7501 tgcgacgacg atgcggccgc ttccgtacgg acgcatcgag aacatcgagg ccttcgacaa
     7561 ggtcatcatg gtcgaccagt cgcccatcgg acgcacgccc cgttcgaacc ctgcgaccta
     7621 tacgggcatc ttctccgcca tccgcgaggt gttcgaacag acgcagactg cgcgcgaacg
     7681 cggctacgcg gcaagccgct tcagcttcaa cgtcaaggga ggccgatgcg aggcctgtca
     7741 gggcgacggc atcgtctgca tgcagatgca gttcctgccc gacgtctacg tgccctgcga
     7801 cgtctgccat ggacgccgct acaacaggga gacgcttgag gtgacctatc agggcaggaa
     7861 catcgccgac gtgctcgaga tgaccgtcga cgaggcgctc ccgttcttcg acgcctatcc
     7921 gaagatcaag cgcgtcctgc aggcgctcgc cgacgtcggc ctcggctaca tcaagctcgg
     7981 ccagagcgcg acgaccttct cgggcggcga ggcccagcgc atcatgctcg ccgccgagct
     8041 gtcgcgaccc gaaacgggcc gaacgctcta catcctcgac gaacccacga cgggccttca
     8101 cttcaaggac atcgagcagc ttctcggcgt cctgcgccgc ctgacggatc agggcaacac
     8161 cgtcctcctc atcgagcaca acctcgacgt cgtcaagtgc gccgattacg tagtcgacat
     8221 gggccccgac ggcggtgaga acggaggcgt catcatggcc gagggcacgc ctgaggaagt
     8281 ggcggagtcc gacagcgtct cgtcgcctta tctgaagcgt gttttataag tcgatataag
     8341 gaaatacatc tgtgtccgtg atgattttgg gtgcgtcggg ccgtctcggc agggcgcttt
     8401 gggagagccg tccgcagacg ctcgaagtga cggcgctgac gcatgcggag cttgatgtaa
     8461 cggacattcg ggcggtcgag gccgtcattg cgctcgctag gcccgacgtc gtgatcaatg
     8521 ccgcggcttg gacggacgtg gcgggcgccc agacgaacgc ggccgctgcg cgggctgtca
     8581 atgccgtggc gcccggcgcc atggggcgtc ttttcgcccg cacgggcgtc aggatcgtgc
     8641 acttctcgac ggactatgtc ttttcgggag agggttcctc accctggaac gaagcttccg
     8701 aggcccatcc cagacaggcg ggcgtctacg gcgtcacgaa gcacgagggg gagaggctcc
     8761 ttgaggaaag cggcgtttcg ggagcgatcg tgcgcgcggg
//
LOCUS       DBMDTI010000064         8708 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2221_length_8708_cov_2.883815, whole genome shotgun sequence.
ACCESSION   DBMDTI010000064 DBMDTI010000000
VERSION     DBMDTI010000064.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2221_length_8708_cov_2.883815"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1840)
                     /gene="ppk1"
                     /locus_tag="ACZPB6_04590"
     CDS             complement(<1..1840)
                     /gene="ppk1"
                     /locus_tag="ACZPB6_04590"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR1183784.1"
                     /translation="MTDSKNPQPEASAATEAAPQQAAQAEAAGKPARRTTARRTARKT
                     AEKTVEKSVEKAGAPVAPKIVDVDDPSLYINREISWIEFDRKVLETAADPTVPLLQRV
                     QFLAIFFNNLDEFYMVRVMNLQRQARMGAEPTGADRMPPARQLTEIRRKVTEMLEVAE
                     DLWLKTLKPALEKKDIKFGRYSQLSPKRRSALDRYFDRQIFPILTPQAVDAGHPFPMI
                     SNTSINFVVELEGVHADEPGKVRFGRVKCPNNVPRFLFLPEDADETTVLDVRSREGTI
                     IMVEDLIMQRMEKLFPGYRVRSQGLFRITRNTDGEIEEDEADDLLAAVRDYVEQRRFG
                     SIVRLELERGMPQCLQDFLVKHLGTRPYQVFKSRMPLAFSEFMGLGGIDRPALKYPPF
                     HPTLPEALEPETDTFAAIRKRDLMFYHPYETFGGVLNFLQKAATDPNVVAIKQTLYRC
                     GSDSPVVRSLLEARRLGKQVTAVVELKARFDEEQNINWAEELEAAGVNVVYGFAGLKI
                     HAKLCLVVRREPEGLRRYVHVGTGNYNAGSAKIYTDLGILTADDAICDDVQDLFNVMT
                     GCGIIDEYRKLFVSPKGTRPNIVRLIEEEVALHKKHDGGHIILKCNQ"
     gene            complement(2040..2729)
                     /locus_tag="ACZPB6_04595"
     CDS             complement(2040..2729)
                     /locus_tag="ACZPB6_04595"
                     /inference="COORDINATES: protein motif:HMM:NF023089.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HIR1183785.1"
                     /translation="MKRLLLPAVLATILAAGCAQNSTPAPYGSCATQPSKPQAVRTSA
                     APELKAKAQAAADDAGKPASCIVAAPLFRPDFRAGVRFDETQRMRFSFSKEAVSEGAP
                     EQIPDFSASIIVKGDSISASLSALNTPFWRISATPSTTAESRHPLLSSRFRAKGLIRD
                     LTFMYWPVDSIRASLAKTCPDCTLSVSGSTRTLARSGQRVLESSTRGGATVLRNYPEG
                     YELSIEPESAR"
     gene            3026..4381
                     /locus_tag="ACZPB6_04600"
     CDS             3026..4381
                     /locus_tag="ACZPB6_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183786.1"
                     /translation="MQNKKLIALAIASLALPAIAAEAPAAALAASTAAPVAAPASAPD
                     SESEDALDSVVNGLASASETGEASPEGEKAEEARTDAAQEVAEGTAGDKTGAADAKSE
                     EKNEDAGAGSADESGADGVAELRTADAVSPLAIVPPFLDFMPYVTSLKDVEEVFGKNA
                     RLYDDGRYGRRHIITGQDFDLGVTSILVYYTDGGLLSDVFLRIPAKERPRVLEALREM
                     TPSMNPDGIWTSDGGRDYWRTKTAELSVGPVKGDDFTVEYGATARQIRETRAWIEADP
                     AHRAPRFAGLSIGISTAADVKAKADGKRCRILGPSHAKDGSSTYALSGPCFGVPGEYQ
                     SLAWIAADSGRLTRLFLQSRGDAVGFSSVLPALEKRYRPTGKPGEFEAGEAPARNVWP
                     AVIRFTPYRKAAGGTVEEGTLEFFAGIDGVKKEEAVWDALLEEKKAREAQARQVDSLF
                     E"
     gene            complement(4475..4954)
                     /locus_tag="ACZPB6_04605"
     CDS             complement(4475..4954)
                     /locus_tag="ACZPB6_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183787.1"
                     /translation="MKKQIMTVAALGAALLAAGCTTNADRRAEQYAEAGGQMEYNIGV
                     VKAEQERIQAEEYYAEQARQKAEAQKQAAKAKADKARAQANAKANAAKKAKQDKLDAL
                     ALRERELKVRLAELKVQEREAQVGTSVAEEAVRTEKAREKVELELERTRAEIKKLDQ"
     gene            complement(4982..5506)
                     /locus_tag="ACZPB6_04610"
     CDS             complement(4982..5506)
                     /locus_tag="ACZPB6_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR1183788.1"
                     /translation="MPQKKLLVIGLFSLTVALSGCATDGNYYRSDVYSSSQVNQAQRV
                     NTVEIIAINPAQVAVNNSENSDRARATGAILGAIAGAAIGNHNNHSTSSRVLGGLAGG
                     AIGGLAGDAVGGSGTTYVDGVQLVYRSSDGQVYQSTQVGRICEYKLGSAIMASSRSGE
                     TRIQPNNPYGCGKR"
     gene            complement(5726..6025)
                     /locus_tag="ACZPB6_04615"
     CDS             complement(5726..6025)
                     /locus_tag="ACZPB6_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183789.1"
                     /translation="MNAVVRNGCCEIRGGAGKPRVTLPPMNIDEAILHRDHVAVVLRN
                     GYCQLYNTEGKLVTTYSVNDAKFVRFVGDDQLAIHRRNGRVEIHDFTGRLVRVSA"
     gene            complement(6341..7015)
                     /locus_tag="ACZPB6_04620"
     CDS             complement(6341..7015)
                     /locus_tag="ACZPB6_04620"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046132 - pyrimidine ribonucleoside
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR1183790.1"
                     /translation="MFKSKIAENILAEHLINNGAVSFLTERPMRLKSGLITPIYVDNR
                     RLTAVPEAWRDILETMATKVVELGLEFDIVAGVEGAGVSHAAALAYRLNKPSIFVRQA
                     AKTYGNKSRVEGCDIAGKRVLLIEDHISTGLSLLSAVEGLKAEGATVTDCLAITSFGI
                     DETNKLFEKQQVRCHEMIDFTKVIDKAIELGTISADKKEVLNDWLANPWTWAARHALT
                     PMDNEN"
     gene            complement(7039..8031)
                     /locus_tag="ACZPB6_04625"
     CDS             complement(7039..8031)
                     /locus_tag="ACZPB6_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1183791.1"
                     /translation="MSTPNAPLTIRDMIEPAIMAAGGWVNTHAHADRAYTLSPDVLEM
                     RRTCTLQQKWDALDALKRNSTEEDFYRRFSMFFENQIAQGVSALATFVDIDPQSEDRA
                     IKAGLRAREHYQDQLTVKFANQTLKGVIHPEARKWFDIGAELVDIIGALPKRDERDYG
                     RGDEAFDIIMETAKSRNKMVHVHVDQFNESLEYETEQLCEKTIQHGMQGKVVAIHGIS
                     IAAHSRAYRQRLYTLMREADVMMIACPTAWIDTPRSEQIGPMHNSMTPVDELVPAGIT
                     VALGTDNVCDAMVPWNAGDMWHEMTTLATGCRFDYFDELVKIATVNGRKAIGID"
     gene            8383..>8708
                     /locus_tag="ACZPB6_04630"
     CDS             8383..>8708
                     /locus_tag="ACZPB6_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1183792.1"
                     /translation="MMGARSVNRRHFRLSMLSFEAEAAVQAEPVPPVLPVLYLDDVMC
                     AVMKPEKLLVHRTEIANGDTVFAVQCVREQLGRRVWPVHRLDRGTSGVLVFACDADTA
                     SRLGRI"
BASE COUNT         1444 a         2432 c         3064 g         1768 t
ORIGIN      
        1 gctggttgca cttgaggatg atgtggccgc cgtcgtgctt cttgtgcagc gcgacctctt
       61 cctcgatgag gcggacgatg ttcggacgcg tgcccttcgg ggagacgaag agcttgcggt
      121 attcgtcgat gatgccgcag cccgtcatca cgttgaagag gtcctggacg tcgtcgcaga
      181 ttgcgtcgtc ggccgtgagg atgccgaggt cggtgtagat cttggcggag ccggcgttgt
      241 agttgccggt gcccacgtgc acgtagcggc gcaggccctc gggttcgcgg cgcacgacga
      301 ggcagagctt ggcgtggatc ttgaggccgg cgaagccgta gacgacgttg acgccggcgg
      361 cttcgagttc ctcggcccag ttgatgttct gctcttcgtc gaagcgggcc ttgagctcca
      421 cgacggccgt gacctgcttg ccgagacggc gggcctcaag gaggctcctc accacgggcg
      481 agtcgctgcc gcagcggtag agcgtctgct tgatggcgac gacgttcgga tcggtcgcgg
      541 ccttctgcag gaagttgagt acgccgccga aggtttcgta cgggtggtag aacatcaggt
      601 cgcgcttgcg gatcgcggcg aacgtgtcgg tttcaggctc gagcgcttcg ggaagcgtcg
      661 ggtggaaggg cgggtacttg agcgcggggc ggtcgatgcc gccgaggccc atgaactcgc
      721 tgaaggcgag cggcattctg gacttgaaca cctgataggg gcgcgtgccg agatgcttga
      781 cgaggaagtc ctgcagacac tgcggcatgc cgcgctcgag ctcgaggcgc acgatggagc
      841 cgaagcggcg ctgctcgacg tagtcgcgca cggccgcgag caggtcgtcg gcctcgtcct
      901 cctcgatttc gccgtcggtg ttgcgggtga tgcggaagag gccctgggag cgcacgcggt
      961 agcccgggaa gagcttctcc atgcgctgca tgatgaggtc ctcgaccatg atgatcgtgc
     1021 cttcgcgcga gcgcacgtca agcaccgtcg tctcgtcggc gtcttcgggc aggaagagga
     1081 agcgcgggac gttgttcggg cacttcacgc ggccgaagcg gaccttgccc ggctcgtcgg
     1141 cgtgcacgcc ttcgagttcg accacgaagt tgatgctcgt gttcgagatc atcgggaagg
     1201 gatggcccgc gtcgacggcc tgcggcgtga ggatcgggaa gatctggcgg tcgaagtagc
     1261 ggtcaagcgc gctgcggcgc ttcggagaga gctgcgagta gcggccgaac ttgatgtcct
     1321 tcttctcgag ggcgggcttg agggtcttga gccagaggtc ttcggcgacc tcgagcattt
     1381 cggtgacctt gcggcggatc tcggtgagct ggcgggcggg cggcatcctg tcggcgcccg
     1441 tgggctccgc acccatgcgg gcctgacgct gcaggttcat cacgcgaacc atgtagaact
     1501 catcgaggtt gttgaagaag attgcgagga actgaacgcg ctgcaacagc ggcaccgtcg
     1561 gatcggcggc cgtctcgagc accttgcggt cgaattcgat ccagctgatc tcgcggttga
     1621 tgtagaggga cggatcgtca acgtcgacga tcttgggggc gacgggagcc ccggctttct
     1681 caacggactt ttcaacggtc ttttcggccg tctttctggc ggtgcgtcgt gcggtcgtgc
     1741 ggcgggcggg cttgcccgcg gcttcggcct gcgcggcctg ctgaggggca gcttcggtcg
     1801 cggcggaggc ttcgggctgg gggttcttgg aatcagtcat gtctctattg tccttgtcta
     1861 gcagcacaac gaatgtcgat tcttatattg tgtgccattt ttcatgacga tgccatgacg
     1921 cattgatgac gtttccctga atcgtgtcac attctgacgt gcgcaggagg aggttcgaag
     1981 acatgcaaaa acggccgcgg atgactgcgg ccgtggcgtt ttgcgtgatt tgacctcgat
     2041 tatcgggcgc tttcgggctc gatggagagc tcgtatccct cagggtagtt ccttagaacc
     2101 gtggcgccgc cccgggtgct cgattcgagc acgcgctggc cgcttcgggc gagtgtgcgg
     2161 gtcgaacccg agacggagag cgtgcagtcg gggcaggtct tggcgaggga ggccctgatc
     2221 gagtccacgg gccagtacat gaaggtgagg tccctgatga ggcccttggc gcggaatctt
     2281 gaggaaagga gcggatggcg ggactcggcc gtcgtcgagg gcgttgcgct gatgcgccag
     2341 aagggcgtgt ttagcgcgga gagggatgcg ctgatcgagt cgcccttgac gatgatcgag
     2401 gcggagaagt cgggaatctg ttcgggggcg ccttcgctca cggcctcctt cgagaaggag
     2461 aacctcatgc gctgcgtttc gtcgaacctc acgcctgcgc ggaagtctgg gcgaaagagc
     2521 ggggccgcca cgatgcagga ggcgggcttg cctgcgtcat cggccgcagc ctgagccttc
     2581 gccttgagtt cgggcgccgc cgacgtgcgc acggcctggg gcttggaggg ctgcgtggcg
     2641 caagatccgt acggagcggg cgtcgaattc tgggcgcatc cggccgcaag gatcgttgca
     2701 aggacggcgg ggaggagaag gcgtttcatg tcggcgggtg acgggcggga ttgaagagcg
     2761 gccacattgt agccgatgca acgatcggaa tacgtaacgg tcagtatcgg tgataaagct
     2821 gaggcagcct gagggcgcct gcgggacagg ctgggaccga gggatgcgtg atgcagggag
     2881 gattgcatgg acggctgaaa gcctcttggc gagagggcca ctcgccgcgc tctccgtgtt
     2941 ccggcgtcaa aatcccgcta atatagaact cgttattgaa gacgcgccgc ccgcccaaga
     3001 gcctgcggcc cacactggtc tcgacatgca gaacaaaaag ctcatcgccc ttgccatcgc
     3061 ctcgctggcc ctgccggcca tcgcggccga agcgcccgcc gcagctctgg cggcgtccac
     3121 cgccgccccc gtggctgcgc ccgcatccgc tccggactcg gagtcggagg acgcgctcga
     3181 cagcgtggtg aacggcctcg cctccgcctc ggagacggga gaggcctcgc ccgaagggga
     3241 gaaggccgaa gaggctcgta cggatgcggc tcaggaagtc gctgaaggaa cggccgggga
     3301 caagaccggg gcggcggacg caaagtctga ggaaaaaaac gaggatgccg gcgcggggag
     3361 tgcggacgag tccggagcgg acggtgttgc cgagctgcgc acggccgatg cggtgtcgcc
     3421 tcttgcgatc gtgccgccgt ttctggactt catgccatac gtgacgagcc tcaaggacgt
     3481 cgaggaggtc ttcggcaaga atgcccgcct ctatgacgac ggacgctacg gcagacgcca
     3541 catcatcacg ggtcaggact tcgatctcgg cgtcacgagc attctcgtct actacacgga
     3601 cggcggactt ctctccgacg tcttcctgag gattcccgcg aaggagcgtc cccgcgtgct
     3661 cgaggccctc agggagatga cgccctcgat gaatcccgac ggcatctgga cttcggatgg
     3721 cgggcgcgac tactggcgca cgaagaccgc agaactctcc gtgggccccg tcaagggcga
     3781 cgacttcacg gtcgagtacg gcgcgacggc gcgccagatc cgcgagacgc gcgcctggat
     3841 cgaggccgat cccgctcacc gcgcaccgcg ctttgcggga ctttcgatcg gcatctcgac
     3901 ggcggccgac gtcaaggcga aggccgacgg caagcgctgc cgcatcctgg ggccgagcca
     3961 tgcgaaggac ggctcctcga cctatgcgct cagcggtccc tgtttcgggg tgccgggcga
     4021 atatcagtcg ctggcgtgga ttgccgccga cagcggcagg ctcacgcgcc tcttcctgca
     4081 gagccgcggc gacgccgtgg gattttcctc cgtgctgcct gcgcttgaaa agcgctacag
     4141 gcccacgggc aagcccggcg aattcgaggc gggcgaggcg cctgcgcgca acgtctggcc
     4201 tgccgtcatc cgcttcacgc cctatcgcaa ggccgcgggc ggcaccgtcg aggagggcac
     4261 ccttgaattc ttcgccggca tcgacggcgt gaagaaagag gaggccgtct gggacgcgct
     4321 tctcgaggag aagaaggcga gggaggctca ggcaaggcag gtcgacagcc tgttcgaatg
     4381 acctcggcaa cggcttgaac aaacgaaagg ccctgcagga gcgaatcctg cggggccttg
     4441 ttcaaggttt caggcctgag ggccttggcc gggctcattg gtcgagcttc ttgatttcgg
     4501 cgcgggtgcg ttcaagctcg agttcgactt tttcacgggc tttctcggtg cggacggcct
     4561 cttcggcaac ggacgtgccg acctgggctt cacgctcctg gaccttcagc tcggcgaggc
     4621 ggaccttgag ctcgcgttcg cgaagggcga gtgcatcaag cttgtcctgc ttggccttct
     4681 tggcggcgtt ggccttggcg ttcgcctggg cgcgggcctt gtcggccttc gccttggcgg
     4741 cctgcttctg ggcttcggcc ttctggcgag cctgttcggc gtagtattcc tcggcctgga
     4801 tgcgttcctg ttcggccttc acgacgccga tgttgtactc catctggccg cctgcttcgg
     4861 cgtactgctc ggcgcggcgg tcggcattgg tcgtgcagcc tgcggcgaga agagcggcgc
     4921 cgagggcggc gaccgtcatg atctgcttct tcatgatgac acttccttca ggaaacccgg
     4981 atcagcgctt gccgcagcca tacgggttgt tcggctggat gcgggtctcg ccgctgcgag
     5041 acgaggccat gatggcggag ccgagcttgt attcgcagat gcgaccgacc tgcgtggact
     5101 ggtagacctg cccgtcggag gagcggtaga cgagctgcac gccgtcgaca taggtcgtgc
     5161 cggagccgcc gacggcgtcg cctgcaagac cgccgatcgc gccgccggca aggccgccga
     5221 ggacgcggga cgaggtcgag tggttgttgt ggttgccgat ggcggcgccg gcgatcgcgc
     5281 cgaggatggc gcccgtggcg cgggcacggt cggagttttc gctgttgttc acggccacct
     5341 gagcgggatt gatggcgatg atttcaaccg tgttcactcg ctgagcctgg ttcacctggc
     5401 tggagctgta gacgtcggaa cgatagtagt tgccgtcggt ggcgcagccg ctgagggcga
     5461 cggtcaggct gaaaaggccg atcacgagga gtttcttctg gggcatggct ttgttcttgt
     5521 cttgggtcga aaaaacggtc attttggact gatcttccgt tcagtccaca tcctcacgat
     5581 acaccattgc gggtgcggcg ggcggatgag ggctgcggtc cgaaacaaga cgttacgcat
     5641 ccttgcgcag gcaggatgca gtggatgtag aaaagccgcc ggtcgctgcg gaatctgcgg
     5701 ctttgcggtg cgtcgcgggc cctgatcagg cggacacgcg gacgagacgg ccggtgaagt
     5761 cgtggatttc gacgcggccg ttgcggcggt ggatggccag ctggtcgtcg ccgacgaagc
     5821 ggacgaactt ggcgtcgttg acactgtacg tcgtgacgag cttgccttct gtgttgtaaa
     5881 gctgacagta gccgtttctc aggacgacgg cgacatggtc gcgatgaagg atggcttcgt
     5941 cgatgttcat cgggggaagc gtgacgcggg gcttgccggc gccgcctctg atctcacagc
     6001 aaccgttgcg gacaactgca ttcattgttt ctctcctcct tggtgggctt cagcatctgc
     6061 atgagggaag aatatgacgg ggtgcgtcag tatgtgacgc aagcagaagc gcgcgcatca
     6121 tgttcgagac tccatcagtt tctgccgaag cttatcgaat caattctacc agaaatgaaa
     6181 tcaggatttc acgcttgtcg ttcgggaggg tgtgcggctg atcttcgcgg cggcgtgcac
     6241 ttgatgcaaa aaaggagcgc tgctttcgca aagcgctcct ttcgactgtc ccgcgcgcgt
     6301 ttacaaagaa cgcgggacta ttcaaaccga acgacggaaa ttagttttcg ttgtccatcg
     6361 gggtgagggc gtgacgagcg gcccaggtcc acggattggc gagccagtcg ttgaggactt
     6421 ccttcttgtc ggcggagatc gtgccgagtt cgatggcctt gtcgatgacc ttcgtgaagt
     6481 cgatcatttc gtggcagcgg acttgctgct tttcgaagag cttgttggtc tcgtcgatgc
     6541 cgaaggaggt gatggcgagg cagtccgtga ccgtggcgcc ttcagccttg aggccttcga
     6601 cggcggagag gagcgagagg ccggtggaga tgtggtcctc aatgaggagg acgcgcttgc
     6661 cggcgatgtc gcagccttcg acgcggctct tgttgccgta ggtcttggcg gcctgacgaa
     6721 cgaagatgct cggcttgttg aggcggtagg ccagagcggc ggcgtgggag acgccggcgc
     6781 cttcgacgcc cgcgacgatg tcgaattcga gaccgagttc gacgaccttc gtggccatag
     6841 tttcaaggat gtcgcgccag gcttccggga cggccgtcag gcggcggttg tcgacgtaga
     6901 tcggcgtgat caggccggac ttgaggcgca tcggacgttc cgtcaggaag gacacggcgc
     6961 cgttgttgat gaggtgttcg gcgaggatgt tttcagcaat cttggacttg aacattttct
     7021 atatctcttt cctgaaaatt agtcgatgcc gatcgccttg cggccgttga ccgtggcaat
     7081 cttgacgagt tcgtcgaagt agtcgaagcg gcagcccgtg gcgagcgtgg tcatttcgtg
     7141 ccacatgtcg ccggcgttcc agggcaccat ggcgtcgcag acgttgtccg tgccgagggc
     7201 gacggtgatg ccggccggga cgagttcgtc gaccggggtc atggagttgt gcatcgggcc
     7261 gatctgttcg gaacgcgggg tgtcgatcca ggcggtcggg caggcgatca tcatcacgtc
     7321 ggcttcgcgc atgagcgtgt agagacgctg gcggtaggcg cgggagtgag cggcgatcga
     7381 gatgccgtga atggcgacga ccttgccctg catgccgtgc tggatggtct tttcgcagag
     7441 ctgctcggtt tcgtattcaa ggctttcgtt gaactggtcg acgtggacgt ggaccatctt
     7501 gttgcgggac ttggccgttt ccatgatgat gtcgaaggct tcgtcgccgc ggccgtagtc
     7561 gcgttcgtcg cgcttcggga gcgcgccgat gatgtcgacg agttcagcgc cgatgtcgaa
     7621 ccacttgcgg gcttccggat ggatcacgcc cttgagggtc tggttggcga acttgacggt
     7681 cagctggtcc tgatagtgtt cgcgggcacg caggccggcc ttgatggcgc ggtcttcaga
     7741 ctgcgggtcg atgtcgacga aggtcgcgag ggcggacacg ccctgggcga tttggttttc
     7801 aaagaacatc gagaagcggc ggtagaagtc ttcttccgtg gagttgcgct tgagggcgtc
     7861 gagggcgtcc catttctgct gaagcgtgca ggtgcggcgc atctcgagaa cgtcggggct
     7921 gagggtgtag gcacggtctg catgagcgtg ggtgttgacc cagccgccgg cggccatgat
     7981 ggccggttcg atcatgtcgc gaatcgtcag gggggcgttt ggcgtagaca ttaagaacat
     8041 ctccttcgca gttagcgaaa acaaaagtgg tatttgccgt cagtactcag gaaaaagccg
     8101 cggatacgtc ggtttgaaag ggatgacgtt tctgcggcta aattgactac attttataga
     8161 tgggttttcg atgtggcgaa tggctacaca aattgttaca tcatcaaggc gtgacgcttg
     8221 tgcttcattc cgttttcttc gctagagccg cagtcgaagg caaatggatg ccggatgctg
     8281 gtcacggact ggggcctcgt gtcggcatgt gaaggtccgc cgttgcgcgt cctgaggcgg
     8341 gttttgtcga cgaatcccgc cgaaaaaggc ccggatttgg gtatgatggg cgcccgttcc
     8401 gtcaaccgac gccatttccg cctctccatg ctgtcattcg aagccgaagc ggccgttcag
     8461 gccgagcccg ttcctcccgt tcttcccgtc ctttatcttg atgacgtgat gtgtgccgtg
     8521 atgaagcccg agaagcttct cgtgcaccgc accgagatcg ccaacggcga cacggtcttt
     8581 gccgtgcagt gcgtgcgtga gcagctcggc cgtcgggtct ggcccgtgca caggcttgac
     8641 cgcgggacgt cgggcgtgct cgtctttgcg tgtgatgcgg acacggcctc gaggctcggg
     8701 cgcatcaa
//
LOCUS       DBMDTI010000065         8696 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2225_length_8696_cov_2.186853, whole genome shotgun sequence.
ACCESSION   DBMDTI010000065 DBMDTI010000000
VERSION     DBMDTI010000065.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8696)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2225_length_8696_cov_2.186853"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="ACZPB6_04635"
     CDS             complement(<1..892)
                     /locus_tag="ACZPB6_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183793.1"
                     /translation="MDADAFWRNRRDNPSQGLERAGHGAVGAPPSAWPLTSIFLNMKF
                     RTYIIVALSATAFVPFVAWSVLHLGDMERRIAEADLEQHSATVDAAALVEERLQGINA
                     VVELADRTLAHSALKEGGDLNLLLQDIVESHPFIKNLHVDHRTADGIRVEAFFPSVSV
                     DGELRRGQNHDNRWHAVLIRAPEGAGTLYSPVLLSSERMPEPLMTFGRRMTGLPEHIL
                     SGALACSPVFEDIRRNLESRGLAFALLDERKQYVWPLDRSRITRWEASLESGTVVDAA
                     AGPVYMTSQKLHAGLPHWTVV"
     gene            1203..2483
                     /locus_tag="ACZPB6_04640"
     CDS             1203..2483
                     /locus_tag="ACZPB6_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HIR1183794.1"
                     /translation="MTLQTIAQLIMLATLILMITGRTPIYMTAITGAAISAIVAGFPL
                     AGGQGMTVTKMIVSGLNPVIADMTGILLFIGIMQATGFLDVIIRDIVRVGNKIGGGVG
                     VGAAGGVAAGVIGALTGFTQPVITAVITGPAAVRLGVKPNQSAGIIAHAGHIGNLAGF
                     THPTQVAILATAGIGYGLFNVLGLIAAGSIFVMSAIRITIDMKRRGVKIDAAEKERIL
                     KEIESREYSTTSWKAFFPFIVLVVGFIAGMPIFLVGLASAMATMILAHSKPKEAEAAM
                     MKGVSLIATPLVATIGFLFMSTVIREIGMVNTMSTLVSPVMSISPVLVLFGVAFLTGF
                     MTQSYAASVAVLVPFLQVTLQAGADPFAAAFAAASGASLIQYFLTGGPVAALATVIPV
                     IPGSDLKGANAFQRPSILFGCLVAFLITFCLNLL"
     gene            2561..3811
                     /locus_tag="ACZPB6_04645"
     CDS             2561..3811
                     /locus_tag="ACZPB6_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183795.1"
                     /translation="MTLELVRSLDVTAVIGISGHAGCGHAHSHCGFVQDDSGGLAAVL
                     ALLQRTTGLDLTITRVTVHTGRKGRFEVETASGGKGSAAARRGITTAEARLAQFVVGR
                     QAICTQALASTAFGRIYGQGAMEVPVALQTAIALAALNSFKVNFPDQVLVADEGVTGN
                     CGRILGTKIRINGVVASVLAVVNASEGGLGPNEDVEGNVNLGPKKALMDKLGLATMPT
                     LLIEGKVCADPASSLISRPTFLIRAYPDDDNVVVAQSYVAAAAKLGYPNLYLDNLLAR
                     SADAMRKLGNSQGENVIRLGKALRDAKTAVEKVRIAAELNEFCSQELGGITFMSEDVH
                     QVMGGVGMIPGTCACLSLFIPHTQLEEDVIPVLSEADAGRFADMVLAAAEDLSKHLPE
                     ACEVIDRIQKRYHEQSQALVEFTL"
     gene            3829..4821
                     /locus_tag="ACZPB6_04650"
     CDS             3829..4821
                     /locus_tag="ACZPB6_04650"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR1183796.1"
                     /translation="MQQKKVIVVATGGTIAMKYDEASQGLIPACSGEDLAAAVPGIDK
                     LAQLEFCQFSNIASPGMTPDDMWRLHLKCEEFLAREDVTGVVVTHGTDTLEETSYFLD
                     ITKISEKPIVTTAAMRGAGDTSPDGPWNIYCSVKTAASEEAVGMGVHVCLNETLHAPG
                     EVTKTHSANPATFQSPWWGPVGYVDLDKIVFRRKTLGVKRFHPEALTARVDLIKAMTG
                     SDREYIDFAVARGCKGIVLEGFGRGNLPPPMIPGVEEAVKKGVAVILTTRTPGGRVLD
                     VYGYPGSVTDSRRVGIAAAGEISAAKARLKLMVVLSEHPEYATNRDKLEEIFDI"
     gene            complement(4970..5704)
                     /locus_tag="ACZPB6_04655"
     CDS             complement(4970..5704)
                     /locus_tag="ACZPB6_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1183797.1"
                     /translation="MDTSAPSLILIVDDETKIRRIVASNLERAGYDVISAADGMHALE
                     IFELQSPKPALVLMDVMMPEMDGFECACRIRELGETPIIFMTAKTDSNSKLKGFNLGA
                     DDYVCKPFSMEELLARIKAVLRRTQAKNTPSESEFTWLVNGPIRICIEKRQCIFNGRE
                     VHLGDIEFRLLSLFVKDPGVLHEHNELLNKVWGANALGEVQYLRVACARLRRKFEDAG
                     LEGGIISAYSGVGYVMRDLRDEDQEV"
     gene            complement(5729..8344)
                     /locus_tag="ACZPB6_04660"
     CDS             complement(5729..8344)
                     /locus_tag="ACZPB6_04660"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1183798.1"
                     /translation="MNVLDVVFQYFEHRIPPSSPNPEEDVYNMRYRNRYFSLFPEDLN
                     KDYNPYDFIEEPVEPRSFRKILTGLIGWTVIIPFILFLLLYQKNIETLSSLVVDRQSL
                     STLVIKTKLDSADGELHNRAIKAINEYLTTSYVEGGLTPAGASEIYRTVQSDLLDAYV
                     RIKDFNTGKTYSFGQPPTDEKPYVTLVPFDATGLQKITMEISMPVDVVAASLREAMKE
                     SGGEAFLVDERGKVLFATDPSLAIENSDFEQIKAGLAHADLHVMDDKLADNKNGVFRT
                     AVKLNQTEYYLIFDQSKAARNLELQHNLIVGSLFFLLAIGSVFVVGYAVSRPLSRSVG
                     ELSDAVGDFPRTGKVEVAMSSDAPTELQELAERFEKMGREIVETQERLRSMNKLLEQK
                     VRSKTDALTTRNAELAAIQKLLAPIDTSLVHLTDETVARFKQLLRLSSLVFRTSASLQ
                     EGNEGPDLPSGAAFVQVRYGNKIYGWLETDAEELKKREVRDSLELLANSLAVQLNNHH
                     LLVSTVQQHQLLEELFSSMNEGVLLMDSNGVLVRFNAYLEERMARDGIALKPGCLVSA
                     ALRQAFEIREKHQDGFSPAPIAKKFEDGRTYELVPVKGAKDDEGGRVYHATAFSVVST
                     NPDAGAPDIGLLVRDVSADAEVERLKDQLISIVAHELKTPITALRLQAETLATQIGLE
                     DEERDQILADMQEESFRLRRLVDDWLDLTRFRDGHISLMPKIMHIATPIDKAAKLVTA
                     RFELKVTRTIDPEAECFKFDPERITQVFINLFSNAARYFREGVPPVVHVDVRRDGDKV
                     RIRVQDNGIGIPKDKATYVFERFYQADMTIARQRGGTGLGLAIVKGIVMAHQGTISLD
                     SELGCGTCFTIELPY"
BASE COUNT         1870 a         2644 c         2208 g         1974 t
ORIGIN      
        1 ccacgaccgt ccagtgcgga agccccgcat gaagcttctg gctcgtcatg tatacgggac
       61 ctgcagccgc atccaccact gttccgctct caagggacgc ctcccatcgc gtaatccgac
      121 tcctgtcgag cggccatacg tactgtttcc tttcgtcaag aagtgcgaag gcaagcccgc
      181 gagattcgag gttccggcgt atgtcctcaa agacaggtga gcacgcaagt gcccccgaca
      241 atatgtgctc gggaagcccc gtcatgcgac gcccgaaggt catcaggggt tcaggcatcc
      301 tttccgagct cagaagcacg ggggaatata gagtccccgc cccttcgggc gcgcgaatga
      361 gaaccgcatg ccaacggttg tcatggttct gaccacggcg cagctccccg tcgaccgaga
      421 cggacggaaa gaaggcttcg acccttatac cgtccgcagt tcgatggtca acgtgcagat
      481 tcttgatgaa cggatgagat tcgacgatgt cctgcaaaag cagattgaga tctcctcctt
      541 ccttcagggc cgaatgtgcg agcgtgcgat cagcgagctc cacgactgca ttgatgcctt
      601 ggagacgctc ctcaaccaga gcagccgcat cgaccgtggc ggaatgttgc tccagatcgg
      661 cttctgcaat acgtctttcc atgtcgccca gatgcagcac gctccaggcg acgaagggaa
      721 cgaaagccgt tgctgaaagc gcaacgatga tgtaggtgcg aaacttcatg ttcaaaaaga
      781 tggatgtcaa cggccatgcc gaaggaggag cgccaacggc gccgtggccg gcgcgttcta
      841 gaccttgaga tggattatcc cgtctattcc tccagaatgc atctgcatcc atcgtagagg
      901 ctggaatcca tgcctaaatt ccccttccgt acgtctccca ctattcgctc tgctctgggt
      961 taaccccata gttaagtgaa cgctttttcc ctatgtgcca cagcatttct acacctgcaa
     1021 tcttttggca acttttttcg gcacgaagga tcggatgttt tattttgccg caatgtttct
     1081 tttttaggct tatgagtgtg atgttcggct tcggcctcat ctactgctat gcatcccctg
     1141 aactgaacca ctttgaaatc aagggatgac tgaaacatcc aaccaatcag gagttaccgc
     1201 atatgactct tcagaccata gcccagctca taatgctggc aacgctcata ttgatgatta
     1261 cgggccgcac gcccatttac atgaccgcca ttacgggtgc ggccatttct gccatcgtgg
     1321 ccggttttcc gcttgcgggt ggtcagggca tgaccgtcac caagatgatc gtctcgggtc
     1381 tcaacccggt gattgccgac atgaccggca ttctgctctt catcggcatt atgcaggcca
     1441 cgggtttcct tgacgtcatc attcgcgaca tcgtccgtgt cggcaacaag attggcggtg
     1501 gcgttggtgt cggcgctgcc ggcggcgtcg ctgctggtgt tatcggcgct ctcaccggtt
     1561 tcacgcagcc cgtgatcacc gctgtcatta ctggtccggc cgccgtccgc cttggcgtca
     1621 agccaaatca gtccgccggt atcatcgccc acgcaggcca catcggcaac cttgccggct
     1681 tcacgcaccc gactcaggtc gccattcttg cgaccgcagg catcggctac ggcctcttca
     1741 atgtccttgg tctcatcgcg gcaggttcca tcttcgtgat gtccgccatc cgcatcacga
     1801 tcgacatgaa gcgccgaggc gtcaagattg acgccgccga aaaagaacgc attctcaagg
     1861 aaatcgaatc ccgcgaatat tcgacgactt cctggaaggc cttcttcccg ttcatcgtcc
     1921 tcgtcgtcgg cttcatcgcc ggcatgccga tcttcctcgt tggcctcgcc tctgccatgg
     1981 ccacgatgat ccttgcgcac tccaaaccga aggaagctga agccgccatg atgaagggcg
     2041 tgtccctcat cgccactccg ctcgtcgcca cgatcggctt cctgttcatg tccaccgtca
     2101 tccgcgaaat cggcatggtc aacacgatgt ccacgctcgt ttctccggtc atgagcatct
     2161 ctccggttct cgtccttttc ggcgtcgcct tccttacggg cttcatgacg cagtcctacg
     2221 ctgcatccgt cgccgttctg gtcccgttcc ttcaggtcac cctacaggcc ggcgccgatc
     2281 cgtttgcagc cgcctttgct gccgcttcgg gcgcctccct tatccagtac ttcctgacgg
     2341 gcggtccagt tgccgctctt gccacggtca ttccggtcat cccgggcagc gacctcaaag
     2401 gagcaaacgc cttccagcgt ccgtcgattc tcttcggttg cctggtggcc ttcctgatca
     2461 ccttctgcct caacctcctc taatgtagac aaagggcggg aagttcttcc ccgaacttcc
     2521 cgctcttgtt gtttaatcaa cgccatcgag gaacagccaa atgacgcttg aactggtcag
     2581 atcacttgat gtgacagccg tgatcggcat ttccggtcac gcgggttgcg gccacgccca
     2641 cagccattgc ggttttgtcc aggatgactc aggcggcctt gccgccgttc tcgcgctcct
     2701 gcagcgcacc acaggactgg acttgacgat cacccgcgta accgttcata caggtcgaaa
     2761 gggccgcttt gaagttgaaa cggcttcggg cggcaagggg agcgcagcgg cccgtcgcgg
     2821 cattacgacc gccgaggccc gtcttgccca gtttgtagtt ggccgacagg caatctgcac
     2881 acaggctctt gcctcaacag ccttcggccg catctacgga cagggcgcca tggaagtacc
     2941 cgtcgcacta cagactgcca tcgctcttgc cgcgctcaac agtttcaaag tcaacttccc
     3001 tgatcaagtc ctcgtcgctg acgaaggcgt gactggcaac tgcggtcgca ttctgggcac
     3061 caagatccgc atcaacggcg tcgtggcctc agttctggcg gtcgtcaacg ccagtgaagg
     3121 aggacttgga ccaaatgagg atgtcgaggg caacgtcaat ctgggcccca aaaaggctct
     3181 catggacaag ctcgggctcg ctaccatgcc cacgcttctc atcgaaggca aggtttgcgc
     3241 cgatccagcc tcatccttga tcagtcgccc caccttcctt attcgcgcat atcctgatga
     3301 cgacaacgtc gtcgtcgcac aaagttacgt tgcagccgca gccaagctcg gctatcccaa
     3361 cctctatctc gacaaccttc ttgcccgcag tgctgacgca atgcgcaagc tcggcaactc
     3421 tcaaggcgaa aatgtcattc gtctcggcaa agctttgcgc gacgccaaaa cggccgttga
     3481 aaaggttcgt attgctgcgg aactcaacga attctgcagt caggagctcg gcggcatcac
     3541 cttcatgtcc gaagatgtgc atcaggtcat gggcggcgta ggcatgatcc ccggcacctg
     3601 cgcctgcctg agcctcttca ttccacacac ccaactcgag gaggatgtca ttcccgttct
     3661 gtccgaggcc gatgcaggca ggtttgccga catggttctt gcagctgcgg aagatctttc
     3721 caaacacctc cccgaagctt gtgaagtcat tgaccgaatt cagaagcgct accatgaaca
     3781 gtctcaagcg ctcgttgaat tcaccctttg aatctctgga gaattgccat gcaacagaag
     3841 aaagtcatcg tcgtcgctac tggcggcacc attgccatga agtacgatga agcgagtcag
     3901 ggtctcatcc ccgcctgctc gggcgaagac cttgccgccg ccgtccctgg catcgacaag
     3961 cttgctcagc tcgagttctg ccagttttcc aacatcgcct ctcctggcat gacgcccgac
     4021 gacatgtggc gcctccacct caagtgcgag gaatttcttg cccgcgaaga cgtgacgggt
     4081 gttgtcgtca ctcacggcac tgacacgttg gaggagacct cttacttcct cgacattacg
     4141 aagatcagcg aaaagcccat cgttacgacc gccgccatgc gcggcgcagg cgacaccagt
     4201 cctgacggtc cctggaacat atactgctcc gtcaagactg ccgcctcaga agaagctgtc
     4261 ggtatgggcg tccatgtctg cctcaatgaa acgctccatg ctccgggcga agtgacgaag
     4321 actcattccg ccaatcccgc cacgttccag tcgccctggt ggggccctgt cggctacgtt
     4381 gacctcgaca agatcgtctt ccgtcgcaag acgcttggcg tcaagcgctt ccatcctgaa
     4441 gccctgaccg cccgagtgga cctcatcaag gccatgacgg gctccgatcg cgaatacatc
     4501 gacttcgccg ttgcccgtgg ttgcaagggc atcgtccttg aaggcttcgg acgcggcaac
     4561 cttccgcctc cgatgattcc tggcgtggaa gaggccgtca agaagggagt tgccgtcatc
     4621 ctgacgacta gaaccccggg tggccgagtt cttgacgttt acggatatcc cggctccgtc
     4681 actgacagcc gccgtgtcgg catcgctgct gcaggagaaa tttccgccgc caaagcccgt
     4741 ttgaagctca tggtcgttct ttccgaacac cctgaatacg caaccaaccg cgacaagctt
     4801 gaggaaatct tcgacatctg attcgcagtc tggggaactg atgaagtccc ctttgagacg
     4861 gccgaccgct gacaagcgcg caacggccgt ccgtttttta tgtgcaaaag gcggcaggaa
     4921 cgcttaactg agcagttctg ccgcctttct cttcttttta cagtgcctat cagacctcct
     4981 gatcctcatc cctcagatcc ctcatgacgt acccaacacc tgaatacgcc gaaatgattc
     5041 ccccctcaag accggcatcc tcaaactttc ttctcaggcg ggcacaggcc acgcgcagat
     5101 actgaacctc acccaatgca ttggcacccc acactttgtt gagaagctca ttgtgttcat
     5161 ggagcacgcc gggatcctta acgaagagtg acaagagcct gaactcaatg tcgccgagat
     5221 gcacttcccg accattgaaa atgcactgac gcttttcaat gcagatccgg attggcccgt
     5281 tgacgagcca cgtgaactcg gattctgacg gggtgttctt cgcctgcgta cgtctcagaa
     5341 ccgccttgat gcgcgccaga agctcctcca tcgagaaggg cttgcagaca tagtcgtcag
     5401 ccccaagatt gaagcccttg agctttgagt tcgaatcggt cttagctgtc atgaagatga
     5461 tcggcgtttc acccagctct cgaattcgac aagcacattc gaagccgtcc atttcaggca
     5521 tcatcacgtc catcagcaca agagcgggct tcggagattg caactcaaaa atttcaagcg
     5581 cgtgcatgcc atccgccgca gagattacgt catagccggc acgctccaga ttcgaggcca
     5641 cgatgcggcg aatcttggtt tcatcatcga caatcaaaat gagtgagggg gctgaggtat
     5701 ccattttcca aattctgtcc tttttaggtt agtacggcag ctcgatggtg aaacaggttc
     5761 cgcatccaag ctcactgtcg agtgaaatag taccctgatg cgccatgaca atgcccttga
     5821 cgatcgccag cccgagacct gttccgcctc tctgtcgcgc gatggtcatg tcggcttgat
     5881 agaagcgctc gaagacatag gtggccttat ccttcggaat gccgatcccg ttgtcctgca
     5941 ctcgaatccg caccttgtcg ccatccctgc gcacgtcaac gtgaacgact gggggaacac
     6001 cttcgcggaa ataacgagcg gcattgctga aaagattgat gaagacctga gtgatacgtt
     6061 cgggatcgaa tttgaaacac tcagcctcag gatcgatggt acgcgtcacc ttgagctcaa
     6121 atcttgccgt gacaagcttt gccgccttgt cgatcggtgt tgcaatatgc atgatcttgg
     6181 gcatgagcga gatgtgtcca tcacggaatc ttgtcagatc gagccagtca tcgacaagcc
     6241 tgcgcagtct gaagctttcc tcctgcatgt ccgcaaggat ctgatcacgc tcctcatcct
     6301 ccagtccgat ctgagtggcc agcgtttcgg cctgaaggcg aagagctgtg atgggagtct
     6361 tgagctcatg agccacgatc gatatgagct gatccttgag gcgctcaacc tccgcgtcag
     6421 cagaaacatc cctcaccaac aacccaatat caggcgcccc cgcatcggga ttggtagaca
     6481 cgacgctgaa ggccgttgcg tgatagaccc ggcccccttc atcatccttg gcacccttga
     6541 ctggtaccag ctcataagtg cgaccatcct cgaacttttt ggcaatcggt gcaggcgaga
     6601 agccgtcctg atgtttttca cgaatttcaa aggcctgccg caacgcagca cttaccagac
     6661 atccaggctt gagagcaatc ccatctctgg ccatgcgctc ctcgagatag gcgttgaaac
     6721 gaaccagcac cccattggaa tccatgagaa gcacgccttc attcattgac gagaacagct
     6781 cctctagaag ctggtgttgc tgcaccgttg acacgagcag atgatgatta ttgagctgaa
     6841 cggcaagcga atttgccaaa agttccaggc tgtcacgcac ctcccgtttt ttcaactcct
     6901 ccgcatcggt ctccagccaa ccgtagattt tgttcccgta acgaacctgc acaaaagccg
     6961 cacctgacgg aagatccggc ccctcgttcc cttcctgaag gctcgcggac gtcctaaaca
     7021 ccagagaaga aagcctgaga agctgcttga agcgcgcaac cgtctcatca gtcagatgta
     7081 ccagtgacgt gtcaatcggt gccagaagtt tctgaatggc ggccagttct gcatttcggg
     7141 tcgtgagagc gtccgttttg gagcgcacct tctgttcaag gagcttgttc atcgaacgaa
     7201 gacgttcctg cgtttctaca atctcccgcc ccatcttctc aaatctttcc gcaagctcct
     7261 gaagctctgt tggtgcgtcc gaagacatcg ccacctcaac cttccctgtc ctcggaaaat
     7321 cacccacggc atcggagagc tcgcctacgc ttcgactcaa cggccggctc acagcgtatc
     7381 cgaccacaaa gacagagccg atggcaagca ggaagaaaag actgccaacg atcaggttgt
     7441 gctgcaactc aagattccta gcagcctttg actggtcaaa aatcaggtaa tactcggttt
     7501 ggttgagttt gacagctgtc ctgaaaacgc catttttgtt gtcggccaac ttgtcatcca
     7561 tcacgtgcag atcggcatga gcaagccctg ccttgatttg ctcgaaatcc gagttttcaa
     7621 tggcaagtga cggatcggtg gcgaaaagca ccttccccct ctcatcgaca agaaaagcct
     7681 ctcccccact ttccttcatc gcctctcgca gcgaggcagc caccacatcg acaggcatgg
     7741 agatctccat ggtgatcttc tgaagccctg tagcgtcaaa gggaacaagc gtgacatatg
     7801 gcttttcatc cgtagggggc tgaccgaaac tgtaggtttt acccgtattg aaatccttga
     7861 tccgcacgta cgcatcgagc aggtcagact gaactgtccg atagatctcg ctcgcacctg
     7921 cgggagtcaa cccaccctcc acataagaag tggtgagata ctcattgatt gccttgatcg
     7981 cccgattgtg cagttcccca tcagctgaat cgagctttgt cttgatgacc aacgtcgaaa
     8041 gtgactgtcg gtccacgact agagacgaca gggtttcgat gtttttttga tacaagagaa
     8101 ggaacaggat aaacggaata atgaccgtcc aaccgatcaa ccccgtcaga atttttcgaa
     8161 agcttcgagg ctctacaggc tcctcgatga agtcgtaggg gttgtagtcc ttgttgaggt
     8221 cctcagggaa gagcgaaaaa tagcggttcc tgtaccgcat gttgtacacg tcttcctcgg
     8281 gattgggact cgagggtgga atcctgtgct caaagtattg gaaaacgacg tcaagaacgt
     8341 tcatggtggt gtcgcttttt gtccggtcat agagtcggac ggggtgcgct cgggattgga
     8401 gtacccacaa ccttagcccg tcccacaaag gttttcaata cagttttccc atgtcctaga
     8461 aagcgcccgt ctcggaccaa cacccgaaaa tgccctccga gagtcgcact gccccctgtc
     8521 cgattctcca gatctggtcg tgaaagttct tcctacacct cccttgagga aagcagtcag
     8581 gcaaagtgct cttcgtcctc ttcgtcgacg gccatgttga agacgtgggc gagctcctcg
     8641 ttcgagaggt ccccgatcca actttccccg gttgcgaccg tcatttcggt gaggcg
//
LOCUS       DBMDTI010000066         8663 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2237_length_8663_cov_2.223312, whole genome shotgun sequence.
ACCESSION   DBMDTI010000066 DBMDTI010000000
VERSION     DBMDTI010000066.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8663)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2237_length_8663_cov_2.223312"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            60..506
                     /locus_tag="ACZPB6_04665"
     CDS             60..506
                     /locus_tag="ACZPB6_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183799.1"
                     /translation="MTKRELVAVLAMGVALFVGGYRYAAAIYGEDIAELREDYASRAQ
                     QLEEKYREKERATAQSLVDAWEARDAALARASDLSGDLDRVRLEADAARRRLSGTTSD
                     TCKLERERLARCSGLVVRGAELVKRGVELSERTAIDKDALTKIVTE"
     gene            complement(935..1261)
                     /locus_tag="ACZPB6_04670"
     CDS             complement(935..1261)
                     /locus_tag="ACZPB6_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR1183800.1"
                     /translation="MLKRITPESEYIFSGSYYNNRSVARYYVSTMVQYASGSTATMHG
                     CRSTFRDWCAREGIDTAVAEKCLMHSLKSVQAAYQRDDLLERRRPVMQQWADAILPMS
                     LLSDHK"
     gene            complement(1271..1900)
                     /locus_tag="ACZPB6_04675"
     CDS             complement(1271..1900)
                     /locus_tag="ACZPB6_04675"
                     /EC_number="2.7.7.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF047080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1183801.1"
                     /translation="MAPLDVRAEHRAEHARRALDFASYAEHALETIFELKHLSEATKV
                     SWRGPMKNHILPVLRHKLVADITVQDICEVLDPLWEKKTTIASRIRGQQEAIFTLAKR
                     EGVYIGENPATWKGNLEAYFPPPARVHRRAHQKAYTIEALTDQLKASLRVDRPVHNAF
                     VFGVLTATRQAEFNDAKWSEIDFEHKVWTVPPERRKDRKPEPFRVPLSD"
     gene            complement(1886..1987)
                     /locus_tag="ACZPB6_04680"
                     /pseudo
     CDS             complement(1886..1987)
                     /locus_tag="ACZPB6_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024750.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(2288..4339)
                     /gene="rbr"
                     /locus_tag="ACZPB6_04685"
     CDS             complement(2288..4339)
                     /gene="rbr"
                     /locus_tag="ACZPB6_04685"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HIR1183802.1"
                     /translation="MSKHIRQEVRVAIDENNPAIVRDEDLCVECRLCAGICNEYVGVN
                     NAYDLAKTGGRSVCIHCGQCIPVCPTNSLQIKSSCDAVRKAVADPDKIVIFSTSPSVR
                     VALGDAFGMADGAFVEGQMVELLRRLGGDYVLDTNFAADLTICEEAAELIERITKAKG
                     PLPQFTSCCPAWVRYCETFHPEMIPHISSAKSPIGMQGPTVKTYFAKKMNLDPKRIVH
                     VAVTPCTAKKAEILREEMNAAGRLLGDPAMRDTDYVITTVELADWAKAARINFAELPE
                     GAFDRLMGEGSGAGVIFGNTGGVMEAALRTAYRHLTGETAPAELYHLTPVRGLQDVKE
                     ATVTIAGNDINVAVIYGTASAGEFIRRLKASETDKPYHFVEVMSCPGGCISGGGQPSA
                     FGNKADVLREARIASLYKRDEEMDCRTSDENEEIKALYRDFYGEPLSELAEELLHTNY
                     TDRRSDLGEKKMSYRCKVCGYIYEGDELPADYICPLCHKGVEVFEKVEEKPAATGEKT
                     LAGTKTEKNLQAAFAGESQARNKYTYFAEVAKREGYEQLAEIFLTTARNEQEHARLWF
                     SALGGIQSTAANLKAAAEGENYEWTDMYVQFAKDAEEEGFPELAAKFRAVGAIERAHE
                     ERYLKLLKNVEMQQVFEKAGECMWECRICGHLVVGTKAPEVCPVCGYSQAYFEVRAEN
                     Y"
     gene            4865..6358
                     /locus_tag="ACZPB6_04690"
     CDS             4865..6358
                     /locus_tag="ACZPB6_04690"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HIR1183803.1"
                     /translation="MFFRKLALTLAVTTALSASALACTTVVVGEGATADGSFLVARAA
                     DSSALKAQHFVIHPAKKNQSGMYRTKDHDGATNFEYPLPKESMRYTTVPNWKTQLHGA
                     VGFNEAGVGISGTESIFARDDALKLDPYNEETGITEDDIHDVVLPRAKTARKGIEILG
                     SIVETIGAGEGFGVVFVDQKEVWYFETGTGHQWMAHRTPKDTYFASGNQGRLQQYDAK
                     SADFMASKTLVSWAEKNGFYDPKKDGEFNFSKAYCRDDARDRVYNDPRVWQIQKLFNP
                     SLEQKPDEGRTYPVYLKPEKKLTVDDMKTMLRNHYEGTDHDPYSPVLNGNEPWRPISV
                     FRTYESHVMHVRPWLPKEIGEVAYVGIGMADLSCYVPYYHGLESYPAHYGMGTDKADS
                     KSIYWKYRKLQTLVMTDYQKLAPVVKAAYKTFEADTAKAQADMEKKYMEVVKKDPKAA
                     SKLLNEFNLKVMNDAEALTDDLTNQVFTIRTTDIQAANFFANNKRKD"
     gene            complement(6475..6921)
                     /locus_tag="ACZPB6_04695"
     CDS             complement(6475..6921)
                     /locus_tag="ACZPB6_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIR1183804.1"
                     /translation="MKEIFPWIFAAALLLAGSAFLYAGVKLTSTAWTLKTSGEQTTAT
                     IERVEVREAHVRKDDAPAIVHYYTEVVSFTAWDGSKVEARLPEKNDAEARGSPGDPIE
                     IRYRHDDPSVAAPVNGSPLGIQGPILIIAGLALLAFGARWTIVKLL"
     gene            7497..8255
                     /locus_tag="ACZPB6_04700"
     CDS             7497..8255
                     /locus_tag="ACZPB6_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HIR1183805.1"
                     /translation="MTLVRTAAALMAATFALGALAAGNTTNEDFRDSKKMLERKVYFD
                     HRVTLYCGYAFDKQKNVSLPAGFVAPQHKERAGRVEWEHVVPAENFGRAFKEWREGDG
                     RCTNEKGHFYKGRRCAEKMSAEYRLMQADMYNLFPAVGAVNAIRSNYNYELLPGVPNT
                     FGTCEMKIDGRRAEPPERSRGEIARAMLYMEGAYAPVFRMSDRQRRLAQNWNEQHPVV
                     QWECTRAARIEKLQGNVNPVVKDACLKAGLTYRP"
BASE COUNT         1697 a         2574 c         2709 g         1683 t
ORIGIN      
        1 aacacctata ggtcgtgatc aacattgtgt atattacaca actttgacgg gagagtcaag
       61 tgacaaagcg ggaattggtt gcggtactcg cgatgggcgt cgctcttttt gtcggtggtt
      121 accgatatgc agcggccatc tacggcgagg acatcgccga actgcgggag gactatgcat
      181 cccgcgcaca gcaacttgag gagaagtacc gtgagaaaga gagagccact gcgcagagct
      241 tggttgacgc atgggaggcg cgcgatgcgg cacttgctcg cgctagtgac ttgtctggcg
      301 accttgacag ggtgcgcctc gaggccgacg cagcccgccg tcgactgtcc ggaaccacct
      361 cagatacctg caagcttgaa agagagcgcc ttgccagatg ctcaggcctt gtcgtcagag
      421 gcgcagaact ggttaaacga ggcgtcgagc tttctgagcg gactgcgata gacaaggacg
      481 ctctgacaaa gatcgtcact gagtgacaaa gttgtagacg aatttggccg aaaatttaga
      541 cgaaggggtg gcgtcaaaat gggttgaaat cgcccgcaat cgttctgccc caacagtttg
      601 agaggtggcg tcaaaaaact tagttagacg aacctttagc cgaagaccaa gttaccaacg
      661 ctggtaaaat ggtcgtaaca acaccgcgca agcctagccg gggacaatcc gtccccggga
      721 agctcactcc gcgcggtttc ctttttattc gtaattgata gcctggggca tcggatctaa
      781 ttatgtagaa ttctacgtaa ttagaaattt ccgccgctcc gcgaggcaaa tttatacggg
      841 ctacgagagt attgatgttc gatcatatgg agcgtcgaga aatcctgccc ctgaaaacgc
      901 cccgcctacc atcatcggta ggtggggcgt ttttctattt gtggtcgctc agcaggctca
      961 tcggcaggat cgcatctgcc cattgctgca tgacgggtcg tctgcgctca agcaagtcgt
     1021 ctctctgata tgcggcctgc actgacttga gcgagtgcat aaggcacttt tctgccactg
     1081 cggtgtcgat gccttcgcgc gcgcaccagt cgcgaaatgt agacctgcag ccatgcattg
     1141 ttgccgtgct gccagaggcg tactgcacca ttgtcgagac atagtagcga gccactgagc
     1201 gattgttgta gtagctgccc gaaaagatgt actcgctctc tggcgtaatg cgcttgagca
     1261 acgcgatcgc ctagtcagac agtgggacgc ggaatggctc gggcttgcgg tctttccttc
     1321 gttcgggcgg aacagtccag accttatgct caaagtcgat ctcggaccat tttgcgtcat
     1381 tgaactcagc ttgtcgcgtg gcggtgagga cgccgaagac gaaagcattg tgcacgggcc
     1441 tgtcgacacg aagactcgcc ttcagctgat cggtgagcgc ctcgatggtg taggccttct
     1501 gatgtgcgcg cctatggacc ctcgccggcg gcgggaagta cgcctccaga ttccctttcc
     1561 aagtggcggg attctcgcca atgtagacgc cttcacgctt cgcgagcgtg aatatcgctt
     1621 cctgctgtcc acggatcctc gatgcgattg tcgtcttctt ttcccacagt gggtctagca
     1681 cttcgcagat gtcctgcacc gtgatgtctg cgactagttt gtgcctgagc actggcagga
     1741 tgtgattctt catcggtcct cgccaagaca ccttggtcgc ttcggacagg tgcttgagct
     1801 cgaagatcgt ttcgagcgcg tgctccgcgt aacttgcaaa gtcgagcgct cgcctggcgt
     1861 gctcggcgcg atgctctgct ctgacatcta aaggggcgat gccgcgcgcg agcagggcgc
     1921 gacacttggc agcttcttgc ttggcggccg tgacggtgac ggtggggtga ctgccgagcg
     1981 acagatctcg ccggcggccc gcaaactgtt agcgaaagat gaaggacgcc ttgcttttcg
     2041 taactcggac ttggagattc gtttcgatcg tgtacctgcc gtccggcagc aggccgatgt
     2101 tttttggggt gacttgcatt ttgcgaagtg agggagagaa tatgaagccc ttctattttg
     2161 tccctcagtc aaatccgtag tttgcaagca aatgagcggt gcgcagaaag taaaaacccc
     2221 gcaagatgac tgtggcacag tcaatcttac ggggtcttca agtatttagc tgttagttag
     2281 ccagtcatca gtagttttca gcgcgcactt cgaaataggc ctggctgtaa ccgcagacgg
     2341 ggcagacttc aggggccttc gtgccgacga cgaggtggcc gcagatgcgg cattcccaca
     2401 tgcattcgcc ggccttttcg aagacctgct gcatctcaac gttcttgagg agcttgaggt
     2461 agcgttcctc gtgggcgcgt tcgatggcgc cgacggcgcg gaacttggcg gcgagttcgg
     2521 gaaagccttc ctcttcggcg tccttggcga actggacgta catgtcggtc cattcgtagt
     2581 tttcgccttc agcggcggcc ttgaggttgg cggccgtgga ctggatgccg ccgagggcgg
     2641 agaaccacag gcgggcgtgt tcctgttcgt tgcgggccgt cgtcaggaag atttcggcga
     2701 gctgctcgta gccttcacgc ttggcgactt cggcgaagta cgtgtacttg ttgcgggcct
     2761 ggctttcgcc tgcgaaggcg gcctggaggt tcttttcggt cttggtgccg gcgagggtct
     2821 tttcgccggt ggcggcgggc ttttcctcaa ccttttcgaa gacctcgacg cccttgtggc
     2881 agagcgggca gatgtagtcg gcgggcagtt cgtcgccttc gtagatgtag ccgcagactt
     2941 tgcaacggta agacattttc ttttctccta aatccgaccg gcggtcggtg tagttggtgt
     3001 gaagcaattc ctcggcgagc tcgctgagcg gttcgccgta gaagtcacgg taaagggcct
     3061 tgatttcctc gttttcgtcg ctcgtgcggc agtccatctc ctcatcgcgc ttgtagaggc
     3121 tcgcaatgcg ggcctcgcga agcacgtcgg ccttgttgcc gaaggcggag ggctggccgc
     3181 cgccggagat gcagccgccg gggcaggaca tcacctcgac gaagtgatag ggcttgtccg
     3241 tctcgctcgc cttgaggcgg cggatgaact cgcctgcgga ggcggtgccg tagatgacgg
     3301 cgacgttgat gtcgttgccg gcgatcgtga cggtggcctc cttgacgtcc tgaaggccgc
     3361 ggacgggggt caggtgatag agctcggcgg gggcggtctc gcccgtcagg tggcggtagg
     3421 cggtgcgaag cgcagcctcc atgactccgc ccgtgttgcc gaagatgacg cccgcgccgg
     3481 agccttcgcc catcaggcgg tcaaaggcgc cctcggggag ctcggcaaaa ttgatgcggg
     3541 cggccttggc ccagtcggcg agctcgacgg tcgtgatgac gtagtcggtg tcgcgcatgg
     3601 cgggatcgcc gagcaggcgg cccgcggcgt tcatctcctc gcgcaggatc tcggccttct
     3661 tggccgtgca gggcgtcacc gccacgtgca cgatgcgctt cgggtcgagg ttcatcttct
     3721 tcgcgaagta ggtcttgacg gtcggaccct gcatgccgat ggggctcttc gcggaggaga
     3781 tgtgcggaat catttcggga tggaaggtct cgcagtagcg gacccaggcg gggcagcagc
     3841 tcgtgaactg gggcagcgga cccttggcct tggtgatgcg ctcgatgagc tcggcagcct
     3901 cctcgcagat cgtaaggtcg gcggcgaagt tcgtgtcgag gacgtagtcg ccgccgaggc
     3961 ggcgaaggag ctcgaccatc tgtccctcga cgaaggcgcc gtcggccatg ccgaaggcgt
     4021 cgccgagcgc cacgcgcacc gacgggctcg tcgagaagat cacgatcttg tcgggatcag
     4081 cgacggcctt cctgacggcg tcgcaggagg acttgatctg aagggagttg gtcgggcaga
     4141 cgggaatgca ctgtccgcag tggatgcaga cggatcgtcc gcccgtcttg gcaagatcgt
     4201 aggcgttgtt gacgccgaca tactcgttgc agatgccggc gcagaggcgg cattcaacgc
     4261 agagatcctc gtcgcggaca atggcgggat tgttctcgtc gatggcgacg cgaacctcct
     4321 gccgaatgtg cttggacata cgctctccta aactgggtgg tctgaatggg gtgttccgca
     4381 accgccgggg gagcggacct ggaacgggca tcctcattgt agcccgtcgc gttcccgact
     4441 gcggcgcggt tttcccgaag cggcggcttc gcgggacgat gatgcatttc catcctgcgt
     4501 gagccggagc gggttttcgc taggtgaaga tgccgtttac cctgagaacg attgcggtag
     4561 cgagatcaag tcaactaaga tgatctgcct agacgacaga cagaccgacg gttcgacgaa
     4621 cggtccgcgc cccgtcgccg tcgcaccttc gcaggcgtcc gccgccacct atatatagag
     4681 gcggatgcag gcgcgccccg tcagcggaca gggcgcgcgg ccccatgcat attccgaacc
     4741 gccggacgtc gcacggggct tgagcacgaa ggagcgggcg cccgatgcag acggttcacc
     4801 cacagccccg cccatccctt ttgaccactc gggcgggact acgtgaaaca tgaggagacc
     4861 agccatgttc tttcgcaagc ttgccctgac gctcgccgtc acgaccgcgc tttccgcctc
     4921 cgccctcgcc tgtaccacgg tcgtggtggg tgaaggcgcc accgccgacg gttccttcct
     4981 cgtcgcgcgt gcggccgaca gcagcgccct caaggctcag cacttcgtca ttcatccggc
     5041 caagaagaac cagtccggca tgtaccgcac caaggaccat gacggtgcga cgaacttcga
     5101 atatccgctc cccaaggaga gcatgcgcta cacgacggtg cccaactgga agacccagct
     5161 gcacggcgcc gtgggcttca atgaagccgg cgtcggcatc tccggcacgg aatcgatctt
     5221 cgcccgcgac gacgccctga agcttgaccc gtacaacgaa gagaccggca tcaccgaaga
     5281 cgacatccat gacgtcgtcc tgccgcgtgc caagaccgcc cgcaaaggca tcgagatcct
     5341 cggctccatc gtcgagacga tcggcgccgg cgaaggcttc ggcgtggtgt tcgtcgacca
     5401 gaaggaagtc tggtacttcg aaaccggcac gggccaccag tggatggctc accgcacccc
     5461 gaaggacacg tacttcgcct ccggcaacca ggggcgcctg cagcaatacg acgccaagag
     5521 cgccgacttc atggcctcca agaccctcgt gagctgggct gaaaagaacg gcttctacga
     5581 tccgaagaag gacggcgagt tcaacttctc gaaggcctac tgccgtgacg acgcccgcga
     5641 ccgcgtctac aacgacccgc gcgtctggca gattcagaag ctcttcaacc cgagcctcga
     5701 acagaagccc gacgaaggcc gtacgtaccc ggtctacctc aagcccgaaa agaagctcac
     5761 cgtcgacgac atgaagacga tgcttcgcaa ccactacgag ggcacggacc acgatccgta
     5821 cagccccgtc ctcaacggca acgaaccgtg gcgtccgatc agcgtgttcc gcacgtatga
     5881 atcccacgtc atgcacgtcc gtccgtggct gccgaaggaa atcggcgaag tcgcctacgt
     5941 gggcatcggc atggccgacc tctcctgcta cgttccgtac taccacggcc tcgaatccta
     6001 cccggcccac tacggcatgg gcacggacaa ggccgacagc aagtcgatct actggaagta
     6061 ccgcaagctc cagacgctcg tcatgacgga ctaccagaag ctcgccccgg tcgtgaaggc
     6121 tgcctacaag acgtttgaag ccgacaccgc caaggcccag gccgacatgg aaaagaagta
     6181 catggaagtc gtgaagaagg acccgaaggc cgccagcaag ctcctcaacg agttcaacct
     6241 caaggtcatg aacgacgccg aggcgctcac ggacgacctg acgaaccagg tcttcacgat
     6301 ccgcacgacg gacatccagg ccgccaactt cttcgccaac aacaagcgca aggactaagc
     6361 gagaccgtct gagccgcctg cgcctcgcct gaggcgcgga cggcgtccga tgaaaacaaa
     6421 atggccccgc aggacatcaa gcctgcgggg ccgttttgtt tcgtgcggcg caagtcagag
     6481 gagcttcacg atcgtccagc gcgcgccgaa ggcgagaagc gccagacccg cgatgatgag
     6541 gatcggcccc tgaatgccga gcggactgcc gttgacggga gcggccaccg agggatcgtc
     6601 gtggcggtag cggatctcga tcgggtcgcc ggggctgccg cgggcctcgg cgtcgttctt
     6661 ctcgggcaga cgagcctcga ccttgctgcc gtcccaggcc gtgaacgaga cgacctcggt
     6721 gtagtagtgc acgatcgcag gcgcatcgtc cttcctgacg tgggcctcgc gaacctcgac
     6781 gcgctcgatc gtcgcggtcg tctgctcgcc gctcgtcttg agcgtccagg cggtggaggt
     6841 gagcttcacg cccgcgtaga ggaaggcgga gccggcaagg agcagcgcgg cggcgaagat
     6901 ccagggaaaa atctctttca tgactgagtc ccaaaatgtc accgcagggc gcggtacctg
     6961 aggctacttt agcaactttc agaaggggtg gagccgcagg acggtgcggg cggatgcttc
     7021 ccgcgcaaaa agagaagccc gcccagcatc gctgctgggc gggctgtcct cagacccatc
     7081 aaaacacgat ctcgaacgca ggagaaagaa cgttcggttt gttcgaggcg gtgctcatcc
     7141 gcctgccact cagcgcgacc cctcacgctg ttttgcagtg gaaccgtgtt tcgttgaaat
     7201 gaactatatc gaacctgaga atcgttcgca aggcaaatac ccgagtaaat gactcgggta
     7261 ttgtaagaaa gtttttccaa gggtttgccc tgtggcggcg tttcttaaga aagcgggctg
     7321 gacgcctgca gcacagtcgt tttggattaa gtcgggcggc aagatgccgc aacatttgcg
     7381 aggggaattt tgagggcggg aggcggtcgg atggggagaa aagggggcgc gagttgcgga
     7441 caatctcggt atgatggacg gacattgaga gaacccgaaa tgggaaggag acataaatga
     7501 ctcttgtccg caccgcggcc gccctgatgg ccgccacgtt cgccctcggc gccctcgctg
     7561 cgggaaacac cacgaacgaa gacttccgtg actcgaagaa gatgctcgag cgcaaggtct
     7621 acttcgacca tcgcgtgacg ctctactgcg gctacgcctt cgacaagcag aagaacgtca
     7681 gtctgcctgc gggcttcgtc gcgccccagc acaaggagcg cgcgggccgc gtcgagtggg
     7741 agcacgtcgt gcccgccgag aacttcggcc gcgccttcaa ggagtggcgc gaaggggacg
     7801 gtcgctgcac gaatgaaaag ggccactttt acaagggccg tcgctgcgcc gagaagatgt
     7861 ctgccgaata ccgcctgatg caggccgaca tgtacaacct gttccccgcc gtcggcgccg
     7921 tcaacgccat ccgctcgaac tacaactacg agctcctgcc cggcgtgccc aacaccttcg
     7981 gcacctgcga gatgaagatc gacggccgcc gcgccgagcc gcccgagcgc tcccgcggcg
     8041 agatcgcccg cgccatgctc tacatggagg gcgcctatgc acccgtcttc cgcatgagcg
     8101 accgccagcg ccgcctcgcc cagaattgga acgagcagca tcctgtcgtg cagtgggagt
     8161 gcacccgcgc cgcacgcatc gagaagctcc agggcaatgt caaccccgtc gtgaaggacg
     8221 cctgcctgaa ggctggtctg acctatcgcc cctaatggct gaatccggcc tcgcccgagg
     8281 ccgggatcct gcccgatcct tcaaaaggga gttgagctcg cgctcggctc cttttttcgc
     8341 atccgttcct ctgacgggtg acggttccgg gaggtgacgc atcggcatcc tcatggatgc
     8401 cggcggacag ttcgccgcga gacgtcaccg cctcgcgttc cattcgggct gagggacgcc
     8461 tgcgccgtct gaaagcgtca gggttaaccc ggtctcttgg gggcgtcatc taggcactta
     8521 cgccaatgca ggttgagcaa tgcggtgttg cggcgctttt tcccgttgaa gtctgttacg
     8581 ccttgctaca cttcgcatca gcctaaaatc cgccactacg tcctcgcggg accgacacct
     8641 gcggggaaga ggattgaagg act
//
LOCUS       DBMDTI010000067         8554 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2255_length_8554_cov_2.622383, whole genome shotgun sequence.
ACCESSION   DBMDTI010000067 DBMDTI010000000
VERSION     DBMDTI010000067.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2255_length_8554_cov_2.622383"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(396..839)
                     /locus_tag="ACZPB6_04705"
     CDS             complement(396..839)
                     /locus_tag="ACZPB6_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183806.1"
                     /translation="MRNSAIIVLCCLGCSSAAAMPGAEPAAGLGDHCQEVEAPCIRQW
                     TEMSASERAHLWPYLDEVSRAMHWRSMSTRERSDMRREMSAADRDKLRKRFCSEQAGA
                     VMSKETRKLRRDERQLLRQQITEFHVQLTGSGEGRAAYAAGASAR"
     gene            1246..1965
                     /gene="ompR"
                     /locus_tag="ACZPB6_04710"
     CDS             1246..1965
                     /gene="ompR"
                     /locus_tag="ACZPB6_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmolarity response regulator transcription
                     factor OmpR"
                     /protein_id="HIR1183807.1"
                     /translation="MERNPKILVVDDDPRLRDLLRRYLGENGFQVFVSENGAAMSRLW
                     VREHFDALILDLMMPGEDGLQILRRLRAAKDMTPVIMLTARGEDVDRIVGLELGADDY
                     IAKPFNPRELLARIHAVLRRRPAGDAPGAPSLENETVRFGEFELDLGTRVLKKNGEVQ
                     PLTTGEFAVLKAFARHPRQPLSRDKLMEMARGREYEAFDRSLDVQVSRLRKLIEPDPS
                     KPRYLQTVWGLGYVFIPDGHS"
     gene            2103..3578
                     /locus_tag="ACZPB6_04715"
     CDS             2103..3578
                     /locus_tag="ACZPB6_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1183808.1"
                     /translation="MLSLRSNHRPSLFWRTFILLCGLIFFSVVGWLQSFRVLSELPYS
                     QGVAQHIVSTANLTKYALVTADPLFRVDLLKLLAAREGLLLSPRDATDKVVVLSGDGF
                     NGLIEQLVKHQLGQDTILASSVNGVSGLWVSVDIDGDAYWMQTDSGILNPPLGTAWLW
                     WALAACLASLFGATLLTRHVIDPLAKLSQVAAQLGQGKVPDPLPEDAGTAEIQAVNMS
                     FNRMVQDLRRMEADRELLLAGVSHDLRTPITRLRLEVELADLPEDSRTAMVSDLEQME
                     NIVNQFLSYARHSHEEQELVNLGEAVQNAMSNARLASDSTVKITSHIAPNVFIVAHPL
                     ELSRAVQNLFTNAMRYGRSEDGILRLHITTGLTDNGKNAMLTVGDEGAGLPEDQRERV
                     MRPFERGESARSGVTGTGLGLAIVDRIVRRSSGSVQLDSMDPHGLLVRVGFPAADPAA
                     IKAKEEKRAREQEKLEKKMLKEFQKNQPAAPADHVDEDPKA"
     gene            complement(3668..4846)
                     /gene="ispF"
                     /locus_tag="ACZPB6_04720"
     CDS             complement(3668..4846)
                     /gene="ispF"
                     /locus_tag="ACZPB6_04720"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR1183809.1"
                     /translation="MSEATKVVGLVCAAGVGSRMGLGSPKQYMKFGSEAMLVHTVRAL
                     RAVERMGEIVVVVSPTDPYIDDVARHFPENVRVLRCGGRERAESVVNGLLNAGFDRDA
                     WVLVHDAARPCLKPSEAARLIDEVLADGGVSGGILAVPVADTIKRADKSRMILETVPR
                     SDLWRAATPQMFRVHDLVEALSGDLAGITDEASAMERLGRPVRIVPGRTTNIKVTEPA
                     DGVLASLILGDGAVVPFRVGQGYDSHRLVEGRSLILGGVQIPFELGLDGHSDADVLLH
                     AVTDAVLGAAALGDIGQHFPPSDPKWKGADSRVLLRAVVALARLKGWIVVNCDATIVA
                     ERPKIGPHMPAIRASLAETLGVSEEVVNVKAKTNERMDAVGRMEGMMAQAVVLMSRMA
                     "
     gene            5025..6674
                     /locus_tag="ACZPB6_04725"
     CDS             5025..6674
                     /locus_tag="ACZPB6_04725"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR1183810.1"
                     /translation="MQSFIHMAGGIALLLWGAYMVKTGMLRTFGVALKDFLSVRLKNR
                     AIAMASGTTLASLLQSSTAATLIVASIQAEGFLTTNMAFATILGADFGSALMTRVLTF
                     DLSFLSPLLIFIGTCLFFMRKADTRQGQFGRILIGLGIIMLALSLIVAATMPLRTSTE
                     LAPLFLEISHSPILAVLLGLCLGFGCFSSLAAVIITSGLVTAGVIPVSTGLWVALGAD
                     IESTILALMTTMTASRIGRRGPVANTMWRTVTLSFTALLLATCPFIAEGFGTIPDSVI
                     YFHVTLNLIISGTGLFIIKPFARIADRIIPEKKADAKTGGEAADLFAKENLISSGMSL
                     NVARTELQRITGDVRNFWHDIEIMLRINPADGEILILHDRGNYINQRTSTMTRYLGLV
                     MRGQLTTAEAIEWQYLKNANGSIKVALNTIDRIITVIMNEKCRKNRSFTPDGLKELQA
                     LHHRVGVCLEQLAVILAEKDLEKRRALCNTLNNEREAILRDSYELTLRHMERVSRGLS
                     GAIDTSALHLELQSLFNRIVGIIGSAASMDYGTPNDPEPQG"
     gene            7059..8279
                     /locus_tag="ACZPB6_04730"
     CDS             7059..8279
                     /locus_tag="ACZPB6_04730"
                     /inference="COORDINATES: protein motif:HMM:NF015316.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR1183811.1"
                     /translation="MKNPTFKIAAAAMMVAGAFATQTYAAGFQLSEQSAIQMGRAMAG
                     AGIVGDDLSAVHYNPAGMTLLSGTRMQATGTWVAVNLDYEGNYGQSENGRLKGQTIPA
                     GFITHQINDSLWAGLGLTVPYGMGTEYGEGWEGRERGTESMILTFDINPNLAWKVNDK
                     LSVGGGISLQYAKAELGMGMGPMQANVKGDSWAWGWNVGLMFQPVETVRLGLAYRSHI
                     AHNAEGHTDVKSLQNLTSDMKVRIKTPDTVTLSATWEATDALRLSGTARWSKWSNFRT
                     LDVQNLDFNTPPLDAYANVPVENNWDDTWFFSVGADYKLNGQWTVRGGVAYDQGPVEN
                     QYRMAVIPDTDRVWFSGGASYKYTDNLTFDFGATYIKGVGDTDLYDKVGGKKIGEFKS
                     LDSYIFSAQMQYLF"
BASE COUNT         1579 a         3002 c         2400 g         1573 t
ORIGIN      
        1 gccgagcgca tcctcgccgc attcggcgag gagctcgtcg atcatggcga ggatgcgctc
       61 ggcatgcttg ttgccgaccg tctcggactt ccaggccgaa acttcgccgt cgcggctcag
      121 aagggccgcg ctgcaccgct ggtgcgccgt atcaagagca agaatgaatg aaggcatgac
      181 cgtccagttg ttttctgcag gcctcgaaaa ggcctccgaa cgcccgaaat cggcgtccca
      241 caatgaattc gatcattata ccatcgagcc tccggcgccc ctcggaggca cgaaaaaggc
      301 cgtccacccc tgcggatgaa cgaccgtttt gccttgtttg gcttgctgtg atcgaatctc
      361 cggccgcccc tgcgtacggc ttgccgtccg aacgtctatc gggccgaggc gccagccgca
      421 taggccgcgc gtccttcgcc gctgcccgtg agctgaacgt ggaattccgt gatctgctga
      481 cgaaggagct gacgttcgtc gcgacgcagc ttgcgcgtct ccttcgacat cacggccccc
      541 gcctgctcgc tgcagaagcg ctttctgagc ttgtcgcggt cggctgcgga catttccctg
      601 cgcatgtcgc tgcgctcgcg ggtgctcatg ctgcgccagt gcatggcgcg ggaaacctcg
      661 tcgagatagg gccacaggtg cgcacgctcg ctcgcggaca tctcggtcca ctggcggatg
      721 catggcgcct cgacctcctg acagtggtcc ccgagacccg cagcgggctc ggccccgggc
      781 atggcggccg ccgaactgca tccgaggcag cagagcacga tgatggcgct gtttctcata
      841 agctagcttc ccgtcatgaa atctgcagac gaattttccg gccttcgcct caaaacgcct
      901 gcgccgttgt cgttcaggga cagcccgcca tttggcgcac aagggttatc cccaaccatg
      961 ttcgcgggat gggttcgaac caccgggccc gaccgtctcc cgaccccgca ccgtcaatat
     1021 acagttcgcc gctcttctag gcagaaacca tgatttagga cctgttaaca agaggctgat
     1081 tttgtaacac gattttgttt tccttccacg aggcatttgt aacaggcgac aatggggtca
     1141 tgcaaaagag tgtccaaaag gcacggctta ctgtccgaca cggctttcgc ggcaatcccg
     1201 cccgaaattc cgatttgcaa cggacgctga ttccggagaa ccaccgtgga acgcaatccc
     1261 aaaattctcg ttgttgacga cgatccccga cttcgcgacc ttctgcgccg ctatctcggc
     1321 gaaaacggct tccaggtatt cgtgagcgaa aacggcgccg cgatgagccg cctctgggtt
     1381 cgcgagcact tcgacgccct cattctcgac ctgatgatgc cgggtgagga cggccttcag
     1441 attctgcgcc gccttcgcgc cgccaaggac atgacgcccg tcatcatgct caccgcccgc
     1501 ggcgaggacg tcgaccgcat cgtcggtctt gagctcggcg ccgacgacta catcgccaaa
     1561 cccttcaacc cgcgcgaact gcttgcccgc atccatgcgg tgctccgccg ccgtccggcg
     1621 ggcgacgccc cgggcgctcc ctcgctggaa aacgagacgg tccgcttcgg cgagttcgag
     1681 cttgacctcg gcacccgcgt actcaagaag aacggcgagg tgcagccgct cacgaccggc
     1741 gagttcgccg tcctcaaggc cttcgcccgc catccgcgcc agccgctctc ccgcgacaag
     1801 ctgatggaaa tggcccgcgg ccgcgaatac gaggccttcg accgctcgct cgacgtgcag
     1861 gtttcccgcc ttcgcaagct gatcgaaccc gatccctcca agccgcgcta cctgcagacg
     1921 gtctggggcc tcggctacgt cttcatcccc gacggccaca gctgatcgcc gggaccgcat
     1981 caggccagtt ctgagaaatt ctgaaagctc ccgtccgcca caagcggcgg gagctttttc
     2041 cgcagtacag aaccgctaca ctttcaccaa acggtttcaa cccacacctg ccggacacac
     2101 tcatgctttc cctacgcagc aaccatcgcc cgagcctctt ctggcgcacc ttcattctgc
     2161 tgtgcggcct catcttcttc agcgtcgtcg gctggctgca gagcttccgc gtgctctccg
     2221 aacttcccta ctcgcagggc gtcgcgcagc acatcgtctc gaccgcaaac ctgacgaagt
     2281 acgcgctcgt gacggccgac ccgctctttc gcgtcgacct gctgaagctt cttgccgccc
     2341 gcgaaggcct tcttctctcg ccgcgcgatg cgaccgacaa ggtcgtcgtc ctctcgggcg
     2401 acggcttcaa cggcctcatc gagcagctcg tcaagcacca gctcggccag gacacgatcc
     2461 tcgcctcgag cgtcaacggc gtctcgggcc tctgggtctc ggtcgacatc gacggcgacg
     2521 cctactggat gcagaccgac agcggcatcc tcaatccgcc gctcggcacc gcctggctct
     2581 ggtgggcgct cgctgcctgt cttgccagcc tcttcggcgc cacgctcctc acccggcacg
     2641 tgatcgatcc gcttgccaag ctctcgcagg tggccgcgca gctcggtcag ggcaaggtcc
     2701 ccgatcctct tcctgaggat gcgggcacgg ccgagatcca ggccgtaaac atgagcttca
     2761 accgcatggt gcaggacctg cgccgcatgg aggccgaccg cgagctgctt ctcgcgggcg
     2821 tgagccacga ccttcgcacg ccgatcacgc gcctgcgcct cgaggtcgag ctcgccgacc
     2881 tgcccgagga ctcccgcacc gccatggtga gcgaccttga gcagatggag aacatcgtca
     2941 accagttcct ctcctacgcg cgtcacagcc atgaggagca ggagctcgtc aacctgggcg
     3001 aggccgttca gaacgccatg tccaacgcca ggcttgcgag cgactcgacc gtcaagatca
     3061 cgagccacat cgcgcccaac gtcttcatcg ttgctcaccc gctcgagctc tcccgcgccg
     3121 tgcagaacct cttcacgaac gcgatgcgct acggccgcag cgaggacggc atcctcaggc
     3181 tccacattac gacgggcctg accgacaacg gcaagaacgc catgctcacc gtcggcgacg
     3241 agggcgcggg ccttcctgaa gaccagcgcg agcgcgtcat gcgacccttc gagcgcggcg
     3301 aaagcgcgcg aagcggcgtg acgggcacgg gcctcggcct cgccatcgtc gaccgcatcg
     3361 tccgccgctc gagcggcagc gtgcagctcg acagcatgga tccgcacggc cttctggtgc
     3421 gcgtaggctt ccccgccgcc gatcccgccg ccatcaaagc caaggaggag aagcgggccc
     3481 gcgaacagga gaagcttgag aagaagatgc tcaaggaatt ccagaagaac cagcccgcag
     3541 cccccgccga tcacgtcgac gaagacccga aggcgtgatg caggaacaag ctgaaagcac
     3601 ctgaaacacg aaggggtcgc tctcacgagc ggcccctttc cctgttggaa cgggcttcag
     3661 attcgcctca agccatcctg ctcatcagca cgacggcctg agccatcatg ccctccatgc
     3721 gtccgacggc gtccatcctc tcattggtct tcgccttgac gttgaccacc tcttcggaga
     3781 cgccaagcgt ctcggcgagc gaggccctga tggcgggcat gtggggaccg atcttcggac
     3841 gttcggccac gatcgtcgcg tcacagttga cgacgatcca gcccttcagt cttgcgagcg
     3901 ccacgacggc cctgaggagc acgcggctgt ccgcgccctt ccacttcgga tccgagggcg
     3961 ggaagtgctg tccgatgtcg ccgagggcgg cagcgccgag cacggcgtcg gtcacggcgt
     4021 gcaggagcac atcggcgtcc gagtggccgt caaggccgag ttcaaacgga atctgcacgc
     4081 cgcccagaat gagcgaacgg ccttcgacca atcgatggga gtcgtatccc tgacctacgc
     4141 ggaagggcac cacggcgccg tctcctaaga tgaggctcgc gagcacgccg tcggcgggtt
     4201 cggtcacctt gatgttggta gtgcgtcccg gcacgatcct gacgggacgg ccgagcctct
     4261 ccatggcgct cgcctcgtcg gtgatccccg cgaggtcgcc cgaaagcgcc tcgacgaggt
     4321 cgtgcacgcg gaacatctgc ggggtggccg cgcgccacag atccgagcgc ggcaccgtct
     4381 cgaggatcat gcggctcttg tccgcacgct tgatcgtgtc ggcgacgggt acggcgagga
     4441 tgccgcccga gacaccgccg tcggcgagca cctcgtcgat gaggcgggcg gcctcgctcg
     4501 gcttcaggca gggtctcgcc gcatcatgca cgagcaccca cgcgtccctg tcgaaccctg
     4561 cgttgagaag gccgttgacg accgactccg cacgctccct gccgccgcag cgcagcaccc
     4621 tgacgttttc aggaaagtgg cgcgccacgt cgtcgatgta gggatccgtc gggctcacga
     4681 ccacgacgat ctcgcccatg cgctcgacgg ctcgaagggc gcgcaccgta tgcacgagca
     4741 tcgcctccga gccgaacttc atgtactgct tgggagagcc gagccccatg cggctgccga
     4801 cgccggccgc gcacacgagc ccgaccacct tcgtggcttc actcatccgt ttctccttct
     4861 tgtcccgtgg cctccgcagg cgcggcaggc cctgatgggc acattgtacc gaggcttagc
     4921 tacaaagcgt tttcccgcag gaagagcact ttgaacggac cttccgctaa tatcgaaaga
     4981 catccgccta ttttcaacac atttttccac ggaggcaggc ttttatgcag tcgttcatcc
     5041 acatggccgg aggcatcgcc ctcctgctct ggggagccta catggtcaag accgggatgc
     5101 tccgcacctt cggtgtggcc ctcaaggact tcctctccgt ccggctcaag aaccgggcca
     5161 tcgccatggc gtcgggcacc acgctcgcat ctcttctcca gagctccacc gccgccacgc
     5221 tcatcgtggc gagcattcag gccgaaggct ttctcaccac caacatggcc ttcgcgacga
     5281 ttctcggcgc cgacttcggt tcggccctga tgacccgcgt gctcaccttc gacctttcgt
     5341 tcctgtcgcc gctcttgatt ttcatcggca cctgcctctt cttcatgcgc aaggcggaca
     5401 cgcgtcaggg ccagttcggc cgcatcctga tcggcctggg catcatcatg cttgcgctca
     5461 gcctcatcgt ggccgccacg atgccgctgc gaacgagcac ggagctcgcg cctctcttcc
     5521 tcgagatctc ccattcgccg atcctcgccg tactgctcgg cctgtgcctc ggcttcggct
     5581 gcttctcgag ccttgccgcc gtgatcatca cctcaggcct cgtcaccgca ggcgtcatcc
     5641 ccgtctccac gggcctctgg gtggcgctcg gcgcggacat cgaaagcacg atccttgcgc
     5701 tcatgaccac gatgaccgcc tcgcgcatcg gccgccgcgg tccggtcgcc aacacgatgt
     5761 ggcgcaccgt gacgctttcg ttcacggccc tgctgctcgc aacgtgcccc ttcatcgccg
     5821 agggcttcgg cacgatcccc gacagcgtca tctacttcca cgtcacgctc aacctgatca
     5881 tctcgggcac gggcctcttc atcatcaaac ccttcgcaag gattgccgac cgcatcatcc
     5941 ccgaaaagaa ggctgatgcc aagacgggcg gcgaggccgc agacctcttc gcgaaggaaa
     6001 acctgatctc gagcggcatg tccctcaacg ttgcgcgcac ggagctgcag cgcataacgg
     6061 gcgacgtccg caacttctgg cacgacatcg aaatcatgct gcgcatcaat cccgccgacg
     6121 gcgagatcct catcctgcat gaccgcggca actacatcaa ccagcgcacg agcacgatga
     6181 cacgctacct cgggctcgtc atgcgcggcc agctcaccac ggccgaggcg atcgagtggc
     6241 agtacctcaa gaacgccaac ggcagcatca aggtcgccct caacacgatc gaccgcatca
     6301 tcacggtcat catgaacgag aagtgccgca agaaccgctc gttcacgccc gacggcctca
     6361 aggagcttca ggccctgcat caccgcgtcg gcgtctgcct cgaacagctt gccgtgattc
     6421 tcgccgagaa ggacctcgag aagcgccgcg ccctctgcaa cacgctcaac aacgagcgcg
     6481 aggcgatcct gcgcgacagc tacgagctga cgttgcgcca catggagcgc gtctcccgcg
     6541 gcctgtcggg cgccatcgac acctccgccc tgcacctcga gctgcagtcg ctcttcaacc
     6601 gcatcgtcgg catcatcggt tcggccgcct ccatggacta cggcaccccc aacgatcccg
     6661 aacctcaggg ctgacacccg ctcgaatcct cggccaatgg cccggtcttc attgaagccg
     6721 ggccattttt gcatccattt tccgaggatc ttcgggctcg tttcgggccc atttcggacc
     6781 attttcaaag tatttcggat taccatgcca ccccttcggg gaaggctcat cgagacgctt
     6841 tccccttcca ttcaattgga acacagggat ttacacgatt tcattcactt cagcgaacat
     6901 catccgcgcg gattaagccg tccatcactt ggggttaacc ctttgaaaga tgattcgttt
     6961 tcggtccttt gattcttcaa aattccctca attcattttt tcgacggcgc ccgtcgcggg
     7021 acgcctcgct cagactttca attgacagga gttttccgtt gaagaacccg actttcaaga
     7081 ttgccgccgc tgcgatgatg gttgccggcg cctttgccac ccagacctac gccgcgggct
     7141 tccagctctc cgagcagtcc gccatccaga tgggccgtgc catggccggc gccggcatcg
     7201 tgggtgacga cctttccgcc gttcactaca atcccgccgg catgacgctt ctctccggca
     7261 cgcgcatgca ggcgacgggc acctgggttg cggtcaatct tgactacgaa ggcaactacg
     7321 gccagagcga aaacggccgt ctcaagggcc agaccattcc cgccggcttc atcacgcacc
     7381 agatcaacga cagcctctgg gcaggtcttg gtctcaccgt cccctacggc atgggcacgg
     7441 aatacggcga aggctgggaa ggcagggagc gcggcaccga gtccatgatc ctcaccttcg
     7501 atatcaaccc gaacctggcc tggaaggtca acgacaagct cagcgtcggc ggcggcatct
     7561 cgctccagta cgccaaggcc gaactgggca tgggcatggg tccgatgcag gcgaacgtca
     7621 agggcgacag ctgggcctgg ggctggaacg tgggcctgat gttccagcct gtcgagaccg
     7681 tccgcctcgg cctcgcctac cgctcgcaca tcgctcacaa cgccgaaggt catactgacg
     7741 tgaagagtct ccagaacctg acgagcgaca tgaaggtccg catcaagacc cccgacacgg
     7801 tcacgctctc cgccacgtgg gaggccaccg acgcgctgcg cctttccggc acggcccgct
     7861 ggtccaagtg gtccaacttc cgcacgctcg acgtccagaa cctcgacttc aacacccccc
     7921 cattggacgc atacgccaac gttcccgtcg aaaacaactg ggacgacacg tggttcttct
     7981 ccgtcggtgc cgattacaag ctcaacggcc agtggactgt ccgcggcggc gtggcctacg
     8041 accaggggcc tgttgaaaac cagtaccgca tggccgtcat tcccgacacc gaccgcgtct
     8101 ggttctcggg cggcgcctcc tacaagtaca cggacaacct gacgttcgac ttcggcgcca
     8161 cctacatcaa gggcgtcggc gacaccgacc tctacgacaa ggtcggcggc aagaagatcg
     8221 gcgaattcaa gtcgctcgac tcctacatct tctccgccca gatgcagtac ctgttctaag
     8281 gtagtccttc aggattaaaa aagcaccgaa aaccctctcc cagagggctt gacggtgctt
     8341 ttcgtctttc aggggactca tcatgctgac ggattagccc gcttctaaaa ctctgttgca
     8401 atgtgttagg gtgcacacct cgttcgggaa aacacgcata aaaagcccga aaagcgacat
     8461 gtgtcgcgca ctccaatcca taccaggagc agatcttgca tcagaatttc atccgcgccg
     8521 ccgctgccgc cgccgtggcc gccaccttct cctc
//
LOCUS       DBMDTI010000068         8509 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2268_length_8509_cov_2.922812, whole genome shotgun sequence.
ACCESSION   DBMDTI010000068 DBMDTI010000000
VERSION     DBMDTI010000068.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2268_length_8509_cov_2.922812"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..61)
                     /locus_tag="ACZPB6_04735"
     CDS             complement(<1..61)
                     /locus_tag="ACZPB6_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR1183812.1"
                     /translation="MDAFYASVEQRDRPELRGRP"
     gene            348..671
                     /gene="fdxA"
                     /locus_tag="ACZPB6_04740"
     CDS             348..671
                     /gene="fdxA"
                     /locus_tag="ACZPB6_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HIR1183813.1"
                     /translation="MPHVVCEACIGCKRTDCVDVCPVDCFREGPNFLVIDPDECIDCA
                     VCIPECPEAAIFAEEDVPEDQQEFIALNAELAREWPSITRRKPYPDDADQYHGVKGKL
                     KFLKR"
     gene            746..1474
                     /locus_tag="ACZPB6_04745"
     CDS             746..1474
                     /locus_tag="ACZPB6_04745"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HIR1183814.1"
                     /translation="MQTVKLTSRREVAPAVYELLFTRPEGWTFEAGQFARIGLAADGD
                     EPVFRAYSIAGSPKAEGLRFLVKSVEGGALSPRLCALNPGDEVLLDGEAQGNLLPARI
                     PGGDTVWMLATGSGLAPFIAMTGDEGTLGAWKKHILVIGARTREEVDRLAAYAESEAR
                     IPLTILTAASREAGELTGRIPALIESGALEAKAGAAFTPESARLLLCGNPDFIRETRA
                     LMKARGIVSPRLGKPGQLLVESLW"
     gene            1499..2119
                     /locus_tag="ACZPB6_04750"
     CDS             1499..2119
                     /locus_tag="ACZPB6_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HIR1183815.1"
                     /translation="MTTLEEIDDLLPQTQCRQCGCDGCLAYARAILEEGAAINRCAPG
                     GTEGIARLAAATGRPVVPLDPEYGREVPFAVARIRARDCIGCAWCIRVCPTDAIAGSP
                     KHLHGIVEERCTGCALCLPACPMDAIDMVENGRTWTLEDAHRAREHHRNAAARRERLE
                     AEDLARLDALRKPAAEKAAGGPSALIADIMARARRANAGDNRGGLT"
     gene            2119..2787
                     /gene="nth"
                     /locus_tag="ACZPB6_04755"
     CDS             2119..2787
                     /gene="nth"
                     /locus_tag="ACZPB6_04755"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR1183816.1"
                     /translation="MKAADRGAFMAALAAAHPNPESELNWTTPFELLVAVVLSAQATD
                     KGVNIATARLFPVANTAEQIAALGVEGLTPFIQTIGLYRNKAKNVVRLSEILRDEYAG
                     EVPEDFDKLVALPGVGRKTANVVLNVAFGHPTIPVDTHIFRVSNRTGLAKGKTPDEIE
                     TKLEKIIPDAYKHEAHHWLLLHGRYCCKARSPECERCPVAQWCNAPEKKKRLADFGSP
                     SKSE"
     gene            3010..4221
                     /locus_tag="ACZPB6_04760"
     CDS             3010..4221
                     /locus_tag="ACZPB6_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR1183817.1"
                     /translation="MTKSCTCHQTPTPSYSQIGKPKESSVIGEILALAARPEVISFAG
                     GLPSAEGFPVKAIKDAADWVLETCPQVALQYSAVGGMPALLEAIAKIETDHGVPTTPD
                     EVMIVSGSQQALDMIARVFTDEGDKVLVETPTYMGALYAFELCKPKFVSLPTDDEGLN
                     PDLIDDRVAGAKFAYVMPTYQNPTGRTISEERRRKLAEKARQYDFWLIEDNPYGELYY
                     AERPPVSLRAFAPERTLRLGTMSKVLAPGFRLGYICGPKHVLNALKQMKTSMDLHTST
                     YTQLITARVLDQDLFSEHLPAVRRLYAEKAKVMLDSLEEFMPKHPEVSWTKPTGGMFI
                     WLNLPKNVDSADLMKKVLASDVPVGFVPGVAFYTDNPEVNHCRLSFVTVPSEKIVAGI
                     KSVAEALKTFL"
     gene            4638..5582
                     /gene="argC"
                     /locus_tag="ACZPB6_04765"
     CDS             4638..5582
                     /gene="argC"
                     /locus_tag="ACZPB6_04765"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR1183818.1"
                     /translation="MTTIFIDGESGTTGLRLRERIANRADLKLISLPADERRSPDARL
                     EAMKAADVTVLCLPDDAAREAVKLADEAGTRVLDASSAHRTAPEWVYGMPELMNGRAA
                     EIARASRVSVPGCHASGFILLVAPLVSAGIIAPDAFLSATSVTGYSGGGKKMISAYET
                     AGRTAFDSLAMPLPYAHSQTHKHLPEMTIAAGLARPPVFQPIVGSFRCGMLVTVPLDL
                     GLLGTTKETVQSVWAKHYAGSPVVRPVEFESLLEDGALRLDAMAGHDGMKIAVSGGPH
                     RVLLTALYDNLGKGSSGAALECLNLMTDSNPARGLSLS"
     gene            5649..6887
                     /gene="argJ"
                     /locus_tag="ACZPB6_04770"
     CDS             5649..6887
                     /gene="argJ"
                     /locus_tag="ACZPB6_04770"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR1183819.1"
                     /translation="MTDSNTAVTFLPDGNVCTPKGFRASGIHCGLRRNKTKKDLALIV
                     SDVPAAAAAVYTLNAVKGAPIAVTKANIADGRAQAMLCNSGNANTCAPNGEAIARKMC
                     KLLAEASGLSADDVIIASTGVIGQPLPIEPIATGMKPLVDALSYEGGEDCAAAIMTTD
                     LVAKHCAVTVDIGGKTVTVGGIAKGSGMIHPNMATMLVFITTDAAISSEMLQKAVSTD
                     VQNTFNMISVDGDTSTNDMLSVMANGLAGNAEITAAGADFDAFAAALHTVTSHLSRAI
                     ARDGEGATKLLTCTVTGATDDATARVLARSVISSSLVKTAMFGSDANWGRVLCALGYS
                     GAPFNPEAADVSFASSAGIVDVCSAGRALDFSEELAKKVLLEKEVSIIVTLHDGDADA
                     TAWGCDLSYEYVRINGDYRS"
     gene            6898..7779
                     /gene="argB"
                     /locus_tag="ACZPB6_04775"
     CDS             6898..7779
                     /gene="argB"
                     /locus_tag="ACZPB6_04775"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR1183820.1"
                     /translation="MNELTDFDIARLLSDSLKYVRLYRSHTVVVKYGGNAMKNPELQQ
                     QVMHDLVLLSMIGIRIVLVHGGGPSINKSLAALGIESKFVRGLRVTDEETMRVVQRVL
                     AGETNKDLVKLLCAEGSKAVGLCGLDAGLITARPEAEDLGLVGHITGVDVSIVETLLE
                     KGCIPVIASVAGDGEGRSYNVNADTAAAAIAGALGARRLVLMTDINGLMRDVKDPDSL
                     ISVLTTEDIGPLKEQGVISGGMIPKIDCALHAIESGVERVAMINGTLPHSILVEMLTK
                     GGIGTLVKTGPRTAASA"
     gene            7821..>8509
                     /locus_tag="ACZPB6_04780"
     CDS             7821..>8509
                     /locus_tag="ACZPB6_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1183821.1"
                     /translation="MKDIKTIDSLYHACTYGRAPVVFERGRGARLWDNKGRDYIDFGS
                     GIGVMSLGYANPEWVKAVSEQAAVLPHTSNLFYNEKTAMLAGRLALRTHFERVFLSNS
                     GAEANECLIKTARKWASDTKGEGEHPFVTLVNSFHGRTLTTVTATGQDTFHKFFGPFT
                     PGFRYVPAGDLAALERQLDAEDCSAVLVEIVQGEGGVRALDADYLKAVEALCHEKNVL
                     FCVDEVQTGNGR"
BASE COUNT         1664 a         3019 c         2411 g         1415 t
ORIGIN      
        1 tcgggcgtcc ccgaagctcg gggcggtcac gctgctcgac cgaagcatag aatgcgtcca
       61 tgtcgacgtg cacgatcctg cgcgagacgt tgcgcacaag cgtgaggcct tcggccccgt
      121 ttcctgcgga aacgcatgcg ggaactgact gtgaagcggg aggacgtgtt gccatggtcg
      181 tcattgtcgg gaaaggggga tttcgaacga caggataaat cagatcgcag ggcctcgagg
      241 cgccgtctac ctccaaattc cgtctttcgg acatggtctg cgggttgcaa aaggactaca
      301 atctctttct tacatttcgt accctcaaat ccaaggagat tccttcaatg cctcatgttg
      361 tctgcgaagc ctgcatcggc tgcaagcgca ccgactgcgt cgacgtctgc cctgtcgact
      421 gcttccgcga aggcccgaac tttctcgtga tcgaccccga cgaatgcatc gactgcgccg
      481 tgtgcatccc cgaatgcccg gaagcagcca tcttcgccga agaggacgtc cctgaggatc
      541 agcaggaatt catcgcgctc aacgccgagc tcgcccgcga atggccctcc attacgcgcc
      601 gcaagcccta ccctgacgac gccgaccagt accacggcgt caagggcaag ctgaagttcc
      661 tcaagcgctg acaagcgctg gcaatggcgg ctcttgcgaa gggccgccat tttttttcat
      721 catcttccac ccggaggctg ccgccatgca gaccgtcaag ctcacgagcc gccgcgaagt
      781 cgcgcccgcc gtttacgaac tcctcttcac ccgtcccgag ggctggacct tcgaggcggg
      841 gcagttcgcc cgcatcggcc ttgccgccga cggcgacgag cccgtcttcc gcgcctactc
      901 gatcgcaggc tcgcccaaag ccgaaggcct gcgctttctc gtgaaatccg tcgaaggcgg
      961 cgcactctca ccccgcctgt gcgccctcaa tccgggcgac gaagtcctgc tcgacggcga
     1021 agcccagggg aaccttctcc ccgcccgcat tccgggcggc gacacggtct ggatgctcgc
     1081 gacgggctcc ggccttgcgc ccttcatcgc catgacgggc gacgaaggca cgcttggcgc
     1141 ctggaaaaag cacatcctcg tcattggtgc tcgcacccgt gaggaggtcg accgcctggc
     1201 cgcctacgcc gagagcgagg ctcggattcc gctcacgatc ctcacggccg cctcccgcga
     1261 agcgggcgaa ctcacggggc gcattcccgc gctcatcgaa tcgggtgccc ttgaagcgaa
     1321 ggccggtgcc gctttcacgc ccgaatccgc ccgtctgctc ctgtgcggca accccgactt
     1381 catccgcgag acccgcgccc tcatgaaggc ccgcggcatc gtatcgccgc gtctgggcaa
     1441 gccgggccag cttctcgtgg aatccctttg gtaagcaacc attccccccg ccagacccat
     1501 gactacgctt gaggaaatcg acgacctgct cccgcagacg cagtgccgcc agtgcggctg
     1561 cgacgggtgc ctcgcctacg cccgcgccat tcttgaggaa ggcgcggcca tcaaccgatg
     1621 cgcgcccggc ggtacggagg gcatcgcccg tcttgccgcc gccacaggcc gccccgtcgt
     1681 gccgctcgat cccgaatacg gacgcgaagt gcccttcgcc gtcgcacgca tccgcgcgcg
     1741 tgactgcatc ggttgcgcct ggtgcatccg cgtctgtccg accgacgcca ttgcaggctc
     1801 tcccaagcac ctgcacggca tcgtggagga gcgttgcacg ggctgcgcgc tctgcctgcc
     1861 agcctgtccc atggacgcga tcgacatggt cgaaaacgga aggacgtgga cgctggagga
     1921 tgcgcacagg gcgagagagc accatcggaa tgccgcagcc cgccgcgagc gcctcgaggc
     1981 cgaggacctc gcccgactcg acgcactccg aaagcctgcg gctgaaaagg ccgccggcgg
     2041 cccctccgcg cttatcgcgg acatcatggc tcgagcccgc cgtgcaaacg ccggcgacaa
     2101 cagaggagga ctgacatgat caaggctgca gaccgcggcg cattcatggc ggcgctcgcc
     2161 gccgcgcacc cgaatcccga gagcgaactc aactggacga cgcccttcga gcttctcgtc
     2221 gccgtcgtgc tctccgcaca ggcgaccgac aagggcgtca acatcgcgac ggccagactc
     2281 tttcctgttg cgaacacggc cgagcagatc gccgcactcg gcgtcgaggg cctcacgccc
     2341 ttcattcaga cgatcggcct ctaccgcaac aaggccaaga acgtcgtgag actctccgaa
     2401 atccttcgcg acgaatacgc gggagaggtg cccgaggact tcgacaagct cgtcgccctg
     2461 cccggcgtgg gccgaaagac ggccaacgtt gtcctcaacg tcgccttcgg acacccgacg
     2521 atccccgtcg acacgcacat cttccgcgtc tcaaaccgca cgggactcgc caagggcaag
     2581 acacccgacg aaattgaaac caagctcgag aaaatcattc ccgacgccta caagcacgag
     2641 gctcaccact ggcttcttct tcacggccga tactgctgca aggcccgctc gcctgaatgc
     2701 gaacgctgcc ccgtcgcgca gtggtgcaac gcccccgaga agaagaagcg ccttgccgac
     2761 ttcggatccc cctcgaagtc agagtgattc ccgttttcca gcgcctatgc agtttgtcct
     2821 agcggctttt ccctagacgg catgacatag acgcagagag gtcgaaataa ccccttcgga
     2881 tgacgcccgg acgatgtccg ggcgtttttt gtctgcctaa aatcacatga aggcgacagg
     2941 ccctcggacg gccgcccccg aacagaaaac ctcgaattcg aacattcagt tgaaaacagg
     3001 aaaagcatca tgacaaagag ctgcacatgc caccaaaccc cgacgccctc ctacagccag
     3061 atcggcaagc ccaaggaaag ctcggtgatc ggcgagatcc tcgccctcgc cgcccgtcct
     3121 gaagtgatca gcttcgccgg cggccttccc tccgccgaag gcttcccggt caaggccatc
     3181 aaggatgcgg ccgattgggt tctggagacg tgtccgcagg tggctctgca gtactccgcc
     3241 gtgggcggca tgcccgcact tctcgaagcc atcgccaaaa tcgagaccga ccacggcgtg
     3301 cccacgacgc ccgacgaagt catgatcgtc tccggctccc agcaggccct cgacatgatc
     3361 gcccgcgtct tcaccgacga gggtgacaag gtcctcgtcg aaacgccgac ctacatgggc
     3421 gctctctatg cattcgagct ctgcaagccg aagttcgtca gcctgccgac cgacgatgag
     3481 ggcctcaacc ccgacctgat cgacgaccgc gtcgcgggcg ccaagtttgc ctacgtgatg
     3541 cccacctacc agaacccgac cggccgcacg atcagcgagg aacgccgccg caagctcgcc
     3601 gaaaaggccc gtcagtacga cttctggctc atcgaggaca atccctacgg cgagctctac
     3661 tacgcagagc gtccgcctgt ctccctgcga gccttcgcac ccgagcgcac gctgcgcctc
     3721 ggcacgatgt ccaaggtgct cgcccccggc ttccgcctcg gctacatctg cggtcccaag
     3781 cacgtcctca acgccctcaa gcagatgaag acctccatgg acctgcacac gagcacctac
     3841 acccagctca tcaccgcccg cgtgctcgac caggacctct tcagcgaaca cctccccgcc
     3901 gtccgacgcc tctacgccga aaaggccaag gtcatgctcg acagcctcga ggagttcatg
     3961 cccaaacacc ccgaagtcag ctggacgaag cccacgggcg gcatgttcat ctggctcaac
     4021 ctcccgaaga acgtcgactc cgccgacctg atgaagaagg tcctcgcgtc cgacgtgccc
     4081 gtcggcttcg tgcccggcgt agccttctac acggacaatc ccgaggtcaa ccactgccga
     4141 ctctccttcg tgacggtccc gtccgagaag atcgtggcgg gcatcaagtc cgtcgccgaa
     4201 gcgctcaaga ccttcctctg agattttccc gatctttctc cccgacacag gcggcgcacg
     4261 ctcaagacgt gcgcccgctc cgccgccaag ggcctgacag gcatcgaccg tcaggccctt
     4321 tcacatactt cacatccgcc ctccggcccg caggcctccc tgcgggcttt gtttttgtcg
     4381 agcagagaca gaaccgaaag cgacccgaaa ggccattttg tcgacaatag atacaccttt
     4441 gaaagacctc ttgttcaatc gaaaaggcca aaactaaggt ctttggagga ggtgttttcg
     4501 catcaagcaa caaaggctcg attagtccgt tcttcattgc gaaatccgcc gttcaactca
     4561 ttacgattgc acagcgcact tccggccccc cctcgaggcc cgccggaatc ccactggcaa
     4621 gacaaaggaa aacacccatg accaccatct tcatcgacgg cgaaagcggc acgacgggcc
     4681 tgaggctccg cgagcggatt gccaacaggg ccgacctcaa gctcatttcg cttcccgcag
     4741 acgagcgccg ctcccccgac gcccgtcttg aggcgatgaa ggcggcagac gtgacggtgc
     4801 tctgcctgcc tgacgacgcc gcacgagagg cggtgaagct ggcagacgaa gccggcaccc
     4861 gcgtgcttga cgcctcgagc gctcacagga ccgctcccga gtgggtctac ggcatgccgg
     4921 aactcatgaa cggccgcgcc gccgaaatcg cgcgcgcatc cagagtatcg gtgccgggct
     4981 gccacgcctc aggcttcatc ctgcttgtgg ctccgcttgt ttccgcaggc atcattgcgc
     5041 cggacgcctt cctttcggcc acatcggtga cgggttattc gggcggcggc aagaagatga
     5101 tctccgccta cgagacggca ggccgcacgg ccttcgacag cctggccatg ccgcttccct
     5161 acgcccattc gcagacgcac aagcaccttc ccgaaatgac gatcgccgca ggtctggctc
     5221 gtccgcccgt cttccagccc atcgtgggga gtttccgctg cggcatgctc gtgacggttc
     5281 cactcgatct tggacttctc ggcacgacaa aggagaccgt tcagtccgtc tgggcaaagc
     5341 actacgcggg ttcccccgtc gtgcgccccg tcgaattcga gtcccttctt gaggacggcg
     5401 ccctgcgcct cgacgcgatg gcgggacacg acggcatgaa gatcgcggtg tcgggcggtc
     5461 cccaccgcgt ccttctcacc gcgctttacg acaacctcgg caaaggctcc tccggagccg
     5521 cgctcgaatg cctgaacctc atgacggact ccaatcccgc ccgagggctc agcctttcct
     5581 aacccggctt cttccagcca ctccgtcgac acgctcgact ccaaacgcac cacgacaaga
     5641 agaacaccat gactgacagc aacaccgccg tgacctttct ccccgatggc aacgtctgca
     5701 ctccgaaggg cttccgggcc tcgggcattc actgcggcct tcgccgcaac aagacgaaga
     5761 aggacctcgc cctcatcgtc tccgacgtgc ccgccgccgc agccgccgtc tacacgctca
     5821 acgccgtcaa gggcgcgccc atcgccgtca ccaaggccaa catcgccgac ggacgcgcgc
     5881 aggcaatgct ctgcaactcc ggcaacgcca acacctgcgc ccctaacggc gaggctatcg
     5941 cccgcaagat gtgcaagctg ctcgccgaag cgtccggcct cagcgcagac gacgtcatca
     6001 tcgcctcgac aggcgtcatt ggccagccgc tcccgatcga gccgatcgcc accggcatga
     6061 agcctcttgt cgacgctctt tcctacgaag gcggcgaaga ctgcgcagcc gcgatcatga
     6121 cgaccgacct cgtggccaag cactgcgcgg tcacggtcga catcggcggc aaaaccgtca
     6181 ccgtcggcgg catcgccaag ggctccggga tgattcaccc gaacatggcc acgatgctcg
     6241 tcttcatcac cacggacgca gcgatctctt ccgagatgct tcagaaggcc gtgagcaccg
     6301 acgttcagaa caccttcaac atgatcagcg tcgacggcga cacgtcgacg aacgacatgc
     6361 tgagcgtcat ggccaacggc cttgcgggca acgccgaaat caccgcggca ggcgcagact
     6421 tcgacgcgtt tgccgccgcg ctccacacgg tcacttctca cctgagccgc gccattgcca
     6481 gagacggcga aggcgcgacc aagctcctca cctgcacggt gacgggcgcg actgatgatg
     6541 cgacggcccg cgtgctagcc aggagcgtca tctcctcgag cctcgtgaag accgccatgt
     6601 tcggttccga cgccaactgg ggccgcgtgc tctgcgcgct cggctacagc ggggcaccgt
     6661 tcaaccccga ggcggccgac gtgtcgttcg cctcctccgc gggcatcgtc gacgtctgca
     6721 gcgcaggtcg cgcgctcgac ttctccgagg agcttgccaa gaaggtgctc cttgaaaagg
     6781 aggtctccat catcgtgacg ctccacgacg gcgatgccga tgcaactgcc tgggggtgcg
     6841 acctctccta tgaatacgtg cgcatcaacg gcgactaccg cagctgaggt caagaccatg
     6901 aacgaactca ccgacttcga catcgcaagg ctcctgagcg actcactcaa gtacgtcagg
     6961 ctctaccgca gccacaccgt cgtggtgaag tacggcggaa acgccatgaa gaaccccgag
     7021 ttgcagcagc aggtgatgca cgacctggtg ctcctctcga tgatcggcat ccgcatcgta
     7081 ctcgttcacg gcggcggtcc ctccatcaac aagtcgctcg ccgcccttgg gatcgaatcg
     7141 aagttcgtgc gcggacttcg cgtcaccgac gaggagacga tgcgggtcgt gcagcgcgtg
     7201 ctcgcaggcg agacgaacaa ggacctcgtc aagctcctgt gcgccgaggg ctcgaaggcc
     7261 gtgggactgt gcggacttga cgcaggtctc atcaccgcac gacccgaagc ggaggatctc
     7321 ggtctcgtgg ggcacatcac gggcgtcgac gtctcgattg tcgagacgct tctcgaaaag
     7381 ggatgcattc ccgtcatcgc gtccgtagcc ggtgacggcg agggccgaag ctacaacgtc
     7441 aacgccgaca cggcggccgc agcgattgcc ggcgcgctcg gcgcgcgccg cctcgtgctg
     7501 atgaccgaca tcaatggact catgcgcgac gtgaaggatc ccgattcgct catctccgtc
     7561 ctcacgacgg aggacatcgg tccgctcaag gaacagggcg tcatttcggg cggcatgatc
     7621 ccgaaaatcg actgcgcgct tcacgccatc gaatcagggg ttgagcgcgt cgccatgatc
     7681 aacggcacgc ttccccacag catcctcgtc gaaatgctca ccaagggcgg catcggtacc
     7741 ctcgtcaaga ccggaccccg cactgctgcg tccgcctgag cggccgcacc acgaacagaa
     7801 ttctctgaag gacattcatc atgaaggaca tcaagaccat cgacagcctt taccacgcct
     7861 gcacctacgg gcgcgcgccc gtcgtcttcg agcgcggccg cggagcccgt ctctgggaca
     7921 acaagggccg cgactacatc gacttcggca gcggcatcgg cgtcatgagc ctcggctatg
     7981 ccaaccccga atgggtgaag gccgtctccg aacaggccgc agttcttccg cacacctcga
     8041 acctcttcta caacgagaag accgccatgc tcgcgggacg ccttgcgctt cgcacgcact
     8101 tcgagcgcgt ctttctttcc aactcaggcg ccgaggccaa cgagtgcctg atcaagaccg
     8161 cccgaaagtg ggcttccgac accaagggag aaggcgagca tcccttcgtc acgctcgtca
     8221 acagcttcca cggccgcacg ctcacgaccg tcaccgccac ggggcaggac acgttccaca
     8281 agttcttcgg gcccttcacg ccgggattcc gctacgtgcc cgcgggcgac cttgccgcac
     8341 tcgaaaggca gcttgacgcc gaggactgct ccgccgttct cgtcgagatc gttcagggcg
     8401 aaggcggcgt gcgcgcgctc gacgcggact acctgaaggc cgtagaggcg ctctgccacg
     8461 aaaagaacgt gctcttctgc gtcgacgaag tgcagacggg caacggccg
//
LOCUS       DBMDTI010000069         8449 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2286_length_8449_cov_4.746526, whole genome shotgun sequence.
ACCESSION   DBMDTI010000069 DBMDTI010000000
VERSION     DBMDTI010000069.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8449)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2286_length_8449_cov_4.746526"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="ACZPB6_04785"
     CDS             complement(<1..704)
                     /locus_tag="ACZPB6_04785"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR1183822.1"
                     /translation="MNKICLAAATAAAAFLLSACSTGSKYYQHDGPPSSSAELDTSSA
                     TPRVEHFYPASLRPYTVMGQRYVPVTSDVKMDQTGIASWYGKQFHGNKTAIGETYNMH
                     AMSAAHPTMPLPSYARITNLENGRSVIVRVNDRGPFLYNRVMDLSYAAATALDYANKG
                     TARVRVERLTNQEIAAGTWQSGNGLTDPVTNSTQVQAPVQSATPSADAVVTAPAAGWS
                     VQIGFFTERDNALAYAA"
     gene            complement(734..1846)
                     /gene="rodA"
                     /locus_tag="ACZPB6_04790"
     CDS             complement(734..1846)
                     /gene="rodA"
                     /locus_tag="ACZPB6_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR1183823.1"
                     /translation="MIETISRTGYAFLRYLATRLLRIDLMLLALVVTLTTIGFIALFS
                     AGYSFPWRIEGQIRNIAVAGVVMLVVSCIPVKAFQKATVPIFIVGCVLLLLTHFFGVT
                     VKGATRWLNVGVRIQPSELMKLAVPMMLAWYYNVRGENVCNWDHLTAFIIMAVPVGLI
                     VKQPDLGTSILVLIAGASVIFFAGLSKRILVSLTGILILSMPVIWSLMHDYQRQRVLT
                     LLDPSADPLGKGFHILQALIAIGSGGFSGKGWMEGTQAHLDFIPERTSDFLFAVFSEE
                     FGFVGNLVLLALYTALIGRSFYIAAFAETRFSRLLAGAIGCIFFTYTFVNMGMVSGIL
                     PVVGVPLPFMSYGGTALLILGVCTGILLSVSAESKH"
     gene            complement(1861..3756)
                     /gene="mrdA"
                     /locus_tag="ACZPB6_04795"
     CDS             complement(1861..3756)
                     /gene="mrdA"
                     /locus_tag="ACZPB6_04795"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR1183824.1"
                     /translation="MALDFKQVDADLVPFRRRLLFVFFLVIVLFCVLIGRFAWLQIIN
                     KNAYTERAEKNRTVTVVSQAARGLIYDRNGELLAKNTLAYSLEITPDKVSDLEKTIDA
                     LSEIIPITTADRRRFVRLREDLNRYDSIPIRQELTDEEVAVFTGQQWRFPGVEINQRE
                     YRVYPEGSVGSHILGYIGSLSQSDKKRLTSEGLIDDYEGERVIGKVGIERSYESLLHG
                     TPGHETLEITAGGHAVRSLAFEPPKAGKNLHLTIDMNLQRVAENAMKGKVGAVIAIQP
                     KTGEILSFVSMPTYDPNLFPGGIDPKSWSQLNTAETKPLLNRAMRGLYPIGSTYKPFV
                     ALAGLETGATTADFVLDDTGVFTLGKHRFRDMSGVPKGRLDLRKSIAVSSDVYYYWLA
                     TRLGVDTIHDFMVPWGFGQKTGIDLVGEQTGVLPSREWKEARVKQPWMVGDTPSIGIG
                     QGYNAFTLLQLAHATSTLANRGIVMTPHLVKRITDPSTNKAEDVPVEPAAVIPLKSRN
                     VETVIAGMTDVSTKGTARFVFKGVPYTVACKTGTAQVVTIAQDDKYDAKKLAKKHHDH
                     ALFIAFAPARNPRIALAVLVENGGFGAQAAAPVARQLIDYWLTGANSLELPPPKGVPL
                     ITQRRNR"
     gene            complement(3772..4311)
                     /gene="mreD"
                     /locus_tag="ACZPB6_04800"
     CDS             complement(3772..4311)
                     /gene="mreD"
                     /locus_tag="ACZPB6_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR1183825.1"
                     /translation="MKQYKTLDMNDGRGGEHIQTRARPLWIISTFLISYLLNLASSTA
                     NVLWLPDPLALTLIYWTIHHPRHVGMTIAFICGLLMDVQNGSVLGQQALAYVILSYSA
                     YSLHRRLPWFGLAGQALHILPLLLVSQLVVLMIRLWFDGLWPGFPWFLQSFTGALLWP
                     AASLILSLPERRSLGRESV"
     gene            complement(4308..5201)
                     /gene="mreC"
                     /locus_tag="ACZPB6_04805"
     CDS             complement(4308..5201)
                     /gene="mreC"
                     /locus_tag="ACZPB6_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR1183826.1"
                     /translation="MEYGPPPLFRQGISARARFLFFVLICVILILVDGRLRSLDNFRS
                     AVISFTTPMVHVATLPITLLGEGEGYFISKVKLKTANDQLLEENQKLSLEVARLREMR
                     EENERLRQLVNAVPREADRVVTGEVLGRVSDQFTRRLQINLGEKSGIVQGMPVIGAFG
                     ILGQITRVASTQSEITLITDHRQRIAVMNERTGERYILAGTGEELMDLLFVQPSADIK
                     GGDRLVTSGLDKIFPRQIMVGTVNETLHKPGETYMAVSVIPSVELEDIQFATVILTNP
                     NVTSVFDKKDKDSTIRRRAGR"
     gene            complement(5286..6329)
                     /locus_tag="ACZPB6_04810"
     CDS             complement(5286..6329)
                     /locus_tag="ACZPB6_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR1183827.1"
                     /translation="MFGFFRNYFSNDLAIDLGTANTLIYMRGKGIVLNEPSVVAIRQE
                     GGPNGKTTIHAVGHTAKQMLGRVPGNINAIRPMKDGVIADFTVTEQMLKQFIRMASPS
                     RLFAPSPRIIICVPCGSTQVERRAIKESALAAGASEVYLIEEPMAAAIGAGLPVNEAA
                     GSLVVDIGGGTTEVGLISLGGMVYAGSVRVGGDKFDQAIINYIRRNFGMLIGEPTAEM
                     IKKKIGSAFPGSEVMEIEVKGRNIAEGVPRTFTVHSNEILEALSEPLNQIVVAVKNAL
                     EKTPPELGADIAEHGLMLTGGGALLRDLDQLLKEETGLPVHVAEEPLNCVVKGCGIAL
                     ENMDKLRTAFAAG"
     gene            6606..6881
                     /gene="gatC"
                     /locus_tag="ACZPB6_04815"
     CDS             6606..6881
                     /gene="gatC"
                     /locus_tag="ACZPB6_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR1183828.1"
                     /translation="MDDIRRIADLGRIDISDEQARIVQGELNDIFQMIERISSVDTTG
                     IAPMPNPHDGVQRLRDDVVSEEVDRSENMKNAPEQAEGCFLVPQVIE"
     gene            6897..8357
                     /gene="gatA"
                     /locus_tag="ACZPB6_04820"
     CDS             6897..8357
                     /gene="gatA"
                     /locus_tag="ACZPB6_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR1183829.1"
                     /translation="MEKDITFASVAELSRMLAAREVSAVELAQHYLARIEAHKDLNAF
                     LDVRPEATLEQARQADERIAAGTATPLTGIPIAHKDIFVTREWASTAASRMLEGYMSP
                     FDATVVRQLKDEGMVCLGKLNCDEFAMGSGNENSAYGKVFNPWDANAVPGGSSGGSSS
                     AVAAGLAPIVTGTDTGGSIREPASFTGVTGMKPTYGRPSRLGMIAFASSLDTAGLLGH
                     SAEDCAWVLGSMVGFDPKDSTSVNMPTEDFKRHLNDGVAGLRIGVPRKWLGEGLDPEL
                     RTSIEEAIETYRRLGAEIVDIELPMAELGVPVYYVVACAEASSNLSRFDGVRYGHRAA
                     DYTDIVDMIKKSRNEGFGAEPKRRIMIGTYVLSHGYYDAYYLKAQKVRRLIAKEFHET
                     FKKVDVIISPVTAGTAYDFGANADPVSAYLSDLYTVPASLAGLPGISVPCGIHSNGRP
                     LGFQLLGETFSEARLLGVAAAWQRETDWHLRRPAGY"
     gene            8379..>8449
                     /locus_tag="ACZPB6_04825"
     CDS             8379..>8449
                     /locus_tag="ACZPB6_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183830.1"
                     /translation="MQWEVVIGLETHVQLSTRSKIFSG"
BASE COUNT         1574 a         2450 c         2844 g         1581 t
ORIGIN      
        1 gcggcgtagg caagcgcgtt gtcgcgctcc gtgaagaagc cgatctgcac gctccagcct
       61 gcggcaggcg cggtcacgac ggcgtcggcg gacggcgtgg cggactgcac gggcgcctga
      121 acctgcgtgg agttcgtgac gggatcagtg aggccgttgc ccgactgcca cgtgcctgcg
      181 gcgatttcct gattggtcag gcgctcgacg cgcacgcgcg cggtgccctt gttggcgtag
      241 tcaagcgccg tggcggccgc gtaggaaaga tccatgacgc ggttgtagag gaagggaccg
      301 cggtcgttga cgcgcacgat gacggagcgc ccgttttcaa ggttggtgat gcgggcgtag
      361 ctcggtagcg gcatcgtggg atgggcggcc gacatggcgt gcatgttgta cgtttcgcct
      421 atggccgtct tgttgccgtg gaactgcttg ccgtaccaag aggcgatgcc cgtctggtcc
      481 atcttgacgt cgctcgtgac gggcacgtag cgctgcccca tgaccgtgta gggacgcagg
      541 gaggccgggt agaagtgctc gacgcgcggc gtcgcgctcg acgtgtcaag ctcggccgac
      601 gaggacggag gaccgtcgtg ctgatagtac ttggaacctg tggaacaggc gctcaacaga
      661 aacgcggcgg cggccgtggc ggcggcgagg cagattttgt tcattgcgcg aagctctttt
      721 ctgatgcggc gtgtcaatgc ttggactcgg cggaaacgga gagcagaatg cccgtgcaga
      781 cgccgagaat gagaagggcc gtgccgccgt agctcatgaa gggcagcggc acacccacca
      841 cgggcaggat accactgacc atgcccatgt tgacgaaggt gtacgtgaag aagatgcagc
      901 cgatggcgcc cgcaaggagg cgggagaagc gcgtttcggc gaaggccgca atgtagaagc
      961 tgcggccgat gagcgcagtg tagagcgcaa gcaggacgag gttgccgaca aagccgaatt
     1021 cctcgctgaa aacggcgaag aggaagtcgc tcgtgcgttc gggaatgaag tcgagatggg
     1081 cctgcgtgcc ttccatccag cccttgcccg agaagccgcc cgagccgatg gcgatgaggg
     1141 cctgcagaat gtggaagccc ttgccgagcg ggtccgcgga cggatcgaga agcgtcagca
     1201 cgcgctgacg ctggtagtcg tgcatcaggc tccagatgac gggcatcgac aggataagga
     1261 tgcccgtgag ggaaacaagg atgcgcttgc tcaggcctgc gaagaagatg acggatgcgc
     1321 ccgcgatgag cacgaggatc gaggtgccga ggtcgggctg cttcacgatg aggccgacgg
     1381 ggacggccat gatgatgaag gccgtcagat ggtcccagtt gcagacgttc tcccctctga
     1441 cgttgtagta ccaggcgagc atcatgggga ccgcaagctt catgagctcc gagggctgga
     1501 tgcgcacgcc gacgttgagc cagcgggtgg cgcccttgac ggtcacgccg aagaaatgcg
     1561 tcagaagcag cagcacgcag ccgacgatga agatcggaac ggtcgccttc tggaaggcct
     1621 tcacgggaat gcaggacacg acgagcatga cgacgcctgc gacggcgatg ttgcgaatct
     1681 gtccctcgat gcgccacggg aagctgtagc cggcggagaa gagcgcgatg aagccgatcg
     1741 tcgtcagcgt gacgacgagg gcgagcagca tcaggtcgat gcgtagcaga cgcgttgcaa
     1801 ggtacctcag gaacgcgtag ccggttctgg aaatggtttc aatcatttca aaatcgctcc
     1861 tcagcggttg cgtcgctggg tgatgagggg aacgcccttc ggaggcggaa gctcgagcga
     1921 attcgcgccc gtgagccagt agtcgatgag ctgtcgggca acgggagcgg ccgcctgggc
     1981 gccaaagccg ccgttttcaa caagaacggc aagcgcgatg cgcggattgc gtgcaggcgc
     2041 gaacgcgatg aagagcgcgt ggtcgtggtg cttcttcgcg agcttcttgg cgtcgtactt
     2101 gtcgtcctgg gcgatggtga cgacctgggc ggtgcccgtc ttgcaggcga ccgtgtaggg
     2161 cacgcccttg aagacgaagc gggccgtgcc tttggtcgag acgtcggtca tgccggcgat
     2221 cacggtctcc acgtttctgg acttgagcgg aatgacggcg gcgggttcga cgggaacgtc
     2281 ctcggccttg ttggtcgacg gatccgtgat cctcttgacg agatggggcg tcattacgat
     2341 gcccctgttg gccagcgtgg acgtggcgtg cgcgagctgc agcagcgtga atgcgttgta
     2401 gccctggccg atgccgatcg acggtgtgtc gcccaccatc cacggctgct tcacgcgcgc
     2461 ctccttccac tccctgctgg gcagaacgcc cgtctgctcg cccacgaggt cgatgcctgt
     2521 cttctggccg aagccccagg gcaccatgaa gtcatggatc gtgtcgacgc ccaagcgggt
     2581 ggcgagccag tagtagtaga cgtcagacga gacggcgatc gacttgcgaa ggtcgagcct
     2641 gcccttgggc acgccgctca tgtcgcggaa cctgtgcttg ccgagcgtga agacgcccgt
     2701 gtcgtcaagg acgaagtccg cagtcgtcgc tcccgtctcc aggcctgcga gcgcaacgaa
     2761 cggcttgtac gtggagccga tcggatagag gccgcgcatg gcgcggttga gaagtggctt
     2821 cgtctcggcc gtgttgagct gggaccagga cttcggatcg atgccgcctg ggaagagatt
     2881 cggatcgtag gtcggcatgg agacgaagga gaggatctca cccgtcttgg gctggatggc
     2941 gatgacggcg cccacctttc ccttcatggc gttttcagcg acgcgctgaa ggttcatgtc
     3001 gatcgtcagg tgcaggttct tgcctgcctt gggcggctcg aaggcgagag agcgcacggc
     3061 atggccgcct gcggtgatct ccagggtctc gtgtcccgga gtgccgtgaa ggaggctctc
     3121 gtagctgcgc tcgatgccga ccttgccgat gacgcgctca ccctcgtagt cgtcgatgag
     3181 gccctcgctg gtcaggcgct tcttgtcgga ctgggacagg gagccgatgt agccgaggat
     3241 gtggctgccg acgctccctt cgggatagac gcggtattcg cgttggttga tctcgacgcc
     3301 gggaaagcgc cactgctggc ccgtgaagac ggcgacctcc tcgtcggtga gctcctgacg
     3361 gatggggatc gagtcatagc ggttgaggtc ttcgcgcagg cgcacgaagc ggcggcggtc
     3421 cgccgtcgtg atgggaatga tctccgagag cgcgtcgatg gtcttctcga ggtcggacac
     3481 cttgtctggc gtgatttcca gagagtaggc gagcgtgttc ttcgcgagca gctcgccgtt
     3541 gcggtcgtag atgaggccgc gggcggcctg cgagacaacc gtgaccgtgc ggttcttttc
     3601 cgcacgctcc gtgtaggcgt tcttgttgat gatctgcagc cacgcgaagc gtccgatcag
     3661 aacgcagaag agcacgatca ccagaaagaa gacgaagagc agacgccttc tgaagggaac
     3721 cagatccgcg tccacctgct tgaagtcaag cgccaccttg ccgactcccc cttaaacgga
     3781 ctcgcgaccg agcgagcgcc tctccggaag ggagaggatg agggaggcgg ccggccacag
     3841 aagggcgccc gtgaagctct ggaggaacca cgggaagccg ggccagaggc cgtcgaacca
     3901 caggcggatc atcaggacga cgagctggga cacgagcagc agcggcagga tgtgcagcgc
     3961 ctggcccgcc agtccgaacc agggcaacct gcggtgcagg gagtaggccg aataggacag
     4021 gatgacgtag gcgagcgcct gctggccgag gacgctgccg ttctgcacgt ccatcagaag
     4081 gccgcagatg aaggcgatcg tcatgcccac gtgacgcgga tggtggatcg tccagtagat
     4141 gagcgtgagc gcgagcggat cgggaagcca gaggacgttc gccgtcgagc ttgccagatt
     4201 gagcaggtag ctgatcagaa acgtgctgat gatccagagc gggcgcgcgc gggtctggat
     4261 gtgctcgccg ccgcgtccgt cattcatgtc aagggtcttg tactgcttca tcgtccggct
     4321 ctgcgcctga tcgtgctgtc cttgtccttc ttgtcgaaga cggacgtgac gttggggttc
     4381 gtcagaatga ccgtggcgaa ctggatgtcc tcaagctcga cactcggaat caccgagacg
     4441 gccatgtagg tttcgccagg tttgtgaagg gtttcgttca cggtgcccac catgatctgc
     4501 cttgggaaga tcttgtcgag gccggacgtg acgagtcggt cgccgccctt gatgtccgcg
     4561 gagggctgca cgaagagcag gtccatcagc tcttcgccgg tgcccgcaag aatgtagcgt
     4621 tcgcccgtgc gctcgttcat gacggcaatg cgctgacggt ggtcggtgat gagggtgatc
     4681 tcggactggg tggaggccac gcgtgtgatc tggccgagaa tgccgaaggc gccgatgacg
     4741 ggcatgccct gcacgatgcc gctcttctcg ccgaggttga tctgcaggcg gcgcgtgaac
     4801 tggtcggaga cgcgaccgag cacttcgccc gtgaccacgc ggtctgcctc gcggggaacg
     4861 gcgttgacga gctgtcgaag gcgctcgttc tcctcgcgca tttcacggag acgcgcgact
     4921 tcgagcgaga gcttctgatt ttcctcgagc agctggtcgt tggcggtctt gagcttgacc
     4981 ttgctgatga agtagccctc gccttcgccg agcagcgtga tgggaagcgt ggcgacgtgc
     5041 accatgggcg tggtgaagct gatcaccgcg ctgcggaagt tgtcgagtga ccgaaggcgc
     5101 ccgtcgacca gaatgaggat gacgcagatc agaacgaaga acaaaaatct ggctcgtgca
     5161 gaaatgcctt gtctgaagag tggaggcggg ccgtattcca tgaagagatg tcctagggat
     5221 ccttgccttc ggttgaaaca aaaaatgcgc ccgggacggt cccgcgggag cagtcctgga
     5281 agtgcttagc ctgccgcgaa ggcggtgcgg agcttgtcca tgttttcaag ggcgatgccg
     5341 cagcccttca ccacgcagtt gagcggttct tcggcgacgt gcacgggcag gcccgtctcc
     5401 tccttgagga gctggtcaag gtcgcgcagc agggcgccgc cgcccgtcag catgaggccg
     5461 tgttcggcga tgtcggcgcc gagttcgggc ggcgtcttct caagggcgtt cttgacggcg
     5521 acgacgatct ggttgagcgg ctcggagagc gcctcgagaa tctcgttgga gtggacggtg
     5581 aaggtgcggg gcacgccttc ggcgatgttg cggcccttga cttcgatttc catgacttcg
     5641 gagccgggga aggcggagcc gatcttcttc ttgatcatct cggccgtcgg ttcgccgatg
     5701 agcatgccga agttgcggcg gatgtagttg atgatggcct ggtcgaactt gtcgccgccc
     5761 acgcggacgg agcctgcgta gaccatgccg cccagcgaga tgaggcccac ttccgtcgtg
     5821 ccgccgccga tgtcgacgac gagggagcct gcagcctcgt tcacgggaag gcccgcaccg
     5881 atggcggcgg ccatcggttc ttcaatcagg tagacctcgg aggcgccggc ggccagagcg
     5941 ctttccttga tcgcgcggcg ctcgacctgc gtggagccgc agggaacgca gatgatgatg
     6001 cgcggagagg gagcgaagag acgggacggg ctggccatgc ggatgaactg cttgagcatc
     6061 tgctcggtca ccgtgaagtc ggcgatcacg ccgtccttca tcggacggat ggcgttgatg
     6121 ttgccgggca cacggccgag catctgcttg gcggtgtggc cgacggcatg aatcgtggtc
     6181 tttccgttgg gaccgccctc ctggcggatg gcaacaacgc tgggttcatt caggacgatg
     6241 cccttgccgc gcatgtagat gagcgtgttg gcggtgccga ggtcaatggc aaggtcattg
     6301 gagaagtaat tgcggaagaa tccaaacatt ttctgttgcc tggtgtaggg tgaagcggcc
     6361 gcttcgaggg agggaatgga acggtgccgg aagcgctgag attcgctgac ggcgcgggtt
     6421 tggcgagacc cagaagcaat ctgttaatga taaactaact gatcgcccgc tggagtgtga
     6481 attaggttcg cgagctttgc tctcttgtgt ttgagtgttt caaagcttaa aaaaatcctt
     6541 ggcgggaact gaatcccatc ctcgaattta aaccgcataa gagaagcacg actatgtccc
     6601 ttacgatgga cgacattcgc cgcattgccg accttggccg tatcgacatt tctgatgagc
     6661 aggccaggat cgttcagggc gaactcaacg atattttcca gatgatagaa cgcatcagct
     6721 ccgtggatac gacgggcatc gctcctatgc ccaatccgca cgacggcgtt cagcgtctgc
     6781 gtgacgacgt cgtctcggaa gaggtggacc gcagcgaaaa catgaagaac gcaccggagc
     6841 aggccgaagg ctgtttcctg gtcccccagg tcattgaata atcgaacgag caggaaatgg
     6901 aaaaggacat tactttcgct tcggtggccg aactgtcccg aatgctggcc gcccgtgaag
     6961 tttcggcggt cgaactcgcc cagcactatc tggcccgcat tgaagcccac aaggatctca
     7021 acgctttcct cgacgtccgt ccggaagcca cgctcgagca ggctcgtcag gccgacgagc
     7081 gcattgccgc gggaacggcc acgccgctca ccggcattcc catcgcccac aaggacatct
     7141 tcgtaacgcg cgaatgggcc tccaccgccg ccagccgcat gctcgagggc tacatgagtc
     7201 cctttgacgc tacggtcgtg cgccagctca aggacgaggg catggtctgc ctcggcaagc
     7261 tcaactgcga cgagtttgcc atgggctcgg gcaatgaaaa ctccgcctac ggcaaggtct
     7321 tcaatccctg ggacgcgaac gccgttccgg gcggctcctc cggcggctcc tcgagcgccg
     7381 tggccgcagg tctcgcgccc atcgtcacag gcaccgacac gggtggctcc atccgcgagc
     7441 cggccagctt caccggtgtg accggcatga agcccacgta cggccgtccg tcccgtctcg
     7501 gcatgattgc cttcgcctcc tcgctcgaca cggctggtct tctcggtcat tcggccgagg
     7561 actgcgcatg ggtgctcggc agcatggtcg gcttcgaccc caaggactcc acgtccgtca
     7621 acatgcccac cgaggacttc aagcgtcacc tcaacgacgg cgtcgccggc cttcgaatcg
     7681 gtgtgccgcg caagtggctc ggcgaggggc tcgatcctga acttcgcacc tccattgaag
     7741 aggctatcga aacgtaccgc cgcctcggtg ccgaaatcgt cgacatcgag ctgccgatgg
     7801 ccgagctcgg cgtgcccgtc tactacgtcg tggcctgtgc cgaagccagc tccaacctgt
     7861 cccgcttcga cggcgttcgc tacggtcatc gcgccgccga ctacacggac atcgtggaca
     7921 tgatcaagaa gtcccgcaac gagggcttcg gtgcggaacc caagcgccgc atcatgatcg
     7981 gaacgtacgt gctttcccac ggctactacg acgcctacta cctgaaggct cagaaggtcc
     8041 gtcgcctgat tgccaaggaa ttccatgaaa ccttcaaaaa ggtcgacgtg atcatcagcc
     8101 ccgtgacggc cggcaccgcc tacgacttcg gcgccaatgc tgaccccgtc tcggcctatc
     8161 tgtccgacct ctacaccgta cctgcctctc tcgcaggcct gcccggcatt tccgtgccgt
     8221 gcggcatcca ttccaacggc cgcccgctcg gcttccagct actcggcgaa acgttcagcg
     8281 aagcccgtct gctcggggtc gcggctgcct ggcagcgtga aacggactgg catcttcgcc
     8341 gtccggccgg ttattgagag tctcaggaga aataaaccat gcagtgggaa gttgtgatcg
     8401 gtcttgagac gcacgttcag ctctcgaccc gttccaagat tttttccgg
//
LOCUS       DBMDTI010000070         8438 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2290_length_8438_cov_3.263284, whole genome shotgun sequence.
ACCESSION   DBMDTI010000070 DBMDTI010000000
VERSION     DBMDTI010000070.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2290_length_8438_cov_3.263284"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(68..152)
                     /locus_tag="ACZPB6_04830"
     tRNA            complement(68..152)
                     /locus_tag="ACZPB6_04830"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Leu,seq:gag)
     gene            complement(318..402)
                     /locus_tag="ACZPB6_04835"
     tRNA            complement(318..402)
                     /locus_tag="ACZPB6_04835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(366..368),aa:Leu,seq:gag)
     gene            complement(468..833)
                     /gene="secG"
                     /locus_tag="ACZPB6_04840"
     CDS             complement(468..833)
                     /gene="secG"
                     /locus_tag="ACZPB6_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR1183831.1"
                     /translation="MHFLLSIALVLQIISAVVVIVLVLMQHGKGADLGAAFGSGASGS
                     VFGATGSANFLSRSTAIAATVFFCSTIALTLASGAFNKRSAQPAGGVLSTVEQPTSHE
                     YGMSGSENPNKAPTNEIPR"
     gene            complement(859..1608)
                     /gene="tpiA"
                     /locus_tag="ACZPB6_04845"
     CDS             complement(859..1608)
                     /gene="tpiA"
                     /locus_tag="ACZPB6_04845"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR1183832.1"
                     /translation="MREKIVIANWKMNGSAEANRLWAEEFLAKLGDLNCRTAVCAPFV
                     YLSELVRLFKDTGVAVGAQTVSPRDQGAFTGEVSAAMLADIGMSLCLVGHSERRTLWG
                     EDDESVAVQAENLVAHGIAPVVCVGETLAEREAGLTEAVVLRQLRTVLTRVPVAKLGA
                     VAYEPVWAIGTGRTATPQAAQHVHAALRACLTEADAEAAAKTPLLYGGSIKPDNAHEL
                     FSQPDIDGGLIGGAALKAEDFHSVCAAARVD"
     gene            complement(1810..3933)
                     /gene="pnp"
                     /locus_tag="ACZPB6_04850"
     CDS             complement(1810..3933)
                     /gene="pnp"
                     /locus_tag="ACZPB6_04850"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR1183833.1"
                     /translation="MTMFNKVSKSFRFGNHDVTLTTGEIARQATGACICQMDDTVVLA
                     TVVAKKETKPGQDFFPLTVDYIEKTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIR
                     PLFPDGFFNEVQVIIHVLSADPAVDPDIPAMIGASAALAISGIPFRGPIGACRVGYIN
                     DEIVINPTTEELKTSRLDLVVAGTERAVLMVESEADILPEKTMLEAVMAGHKAMQVCI
                     QAINELVAEAGKPAWDWQPAPKNEALVARIVELCDAGLKEAYGIRSKQARTEKLREVY
                     AACEAQLVADAEAAGTEAPDMNEVHGILFELEAKLVRSNILNGEPRIDGRDTRTVRPI
                     EIRQSVLPRTHGSALFTRGETQALVLTTLGTKQDEQIIDGLCEETHDRFMLHYNMPPF
                     ATGETGRVGSPKRREIGHGRLAKRALKAVLPTAEEFQYTIRVVSEITESNGSSSMASV
                     CGGCLSMLDAGVPLKDYVAGVAMGLIKEGNKFAVLTDILGDEDHLGDMDFKVAGTAEG
                     VTALQMDIKIEGITAEIMQAALAQAHEGRQHILGKMHEMAGGGAKELSDFAPRMISFK
                     IDQDKIREVIGKGGATIRALTEETGTTINIEDDGTVTIASPDTARVEEARRRIEIITA
                     KIEVGQIYEGTVQRLLDFGAIVQLLPGKDGLLHISQIANERVNQVSDYLKEGQQVRVK
                     VIEADEKGRVRLSMKALLKDEAAQA"
     gene            complement(4196..4462)
                     /gene="rpsO"
                     /locus_tag="ACZPB6_04855"
     CDS             complement(4196..4462)
                     /gene="rpsO"
                     /locus_tag="ACZPB6_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR1183834.1"
                     /translation="MSVEMNKSEIVAKFQRAEGDTGSPEVQVALLTARINHLTDHFKV
                     HTKDYHSRRGLLRMVSRRRKLLDYLKRTDLNAYRALIEALNLRK"
     gene            complement(4718..5383)
                     /locus_tag="ACZPB6_04860"
     CDS             complement(4718..5383)
                     /locus_tag="ACZPB6_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1183835.1"
                     /translation="MEFDLSLIVNSLPLLLKGAGLTLEITALAVGLGLIIGLFLGLAQ
                     LSKNPIFRWPAKVYVDVIRGTPLLIQIFIIYFALPNIIGERIDPYIAAVAACSLNSGA
                     YIAEIFRAGIQSISLGQMRAGLSLGMTYPQTMRYIVLPQAVKRIIPPLGNEFIAMLKD
                     SSLVSVIGFEELTRSGQLVIAETYGTMEIWTCVAILYLVMTLTISQLVGWLEKRYRLS
                     DER"
     gene            complement(5642..6421)
                     /locus_tag="ACZPB6_04865"
     CDS             complement(5642..6421)
                     /locus_tag="ACZPB6_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1183836.1"
                     /translation="MQFKLARAGVKTFAGVIMSAAVLGGMSANAFAERLIKVATEPTF
                     PPFEFVNTQTGEVSGFEMDLVRAVGKELGAKIEFQVLSFDAIIPAMLSGTVEMGAAGF
                     SITEERKKRVLYSDTFYTSGLSLVTTKAKASAVKSMKDLEGKTIGVQIGTTSHNAAKK
                     VKGAKIVTFNTAGEAILDLSIGGSDAVINDRPVTAYIMTQQPKLGAQLVHQPETYSAD
                     DFAFVFRKDQSALKDEVNAALAKLKSNGEYDKIYNKWFGKN"
     gene            complement(6654..7409)
                     /locus_tag="ACZPB6_04870"
     CDS             complement(6654..7409)
                     /locus_tag="ACZPB6_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1183837.1"
                     /translation="MMFKKVFAAAASALAIAAFAGNAMARDTLRVGTEPTFAPFEFLD
                     TKTQEFSGFDIDLIKAVADKAGYDVKVMNMGFDALIPALSAGTIDVIASGISITEERQ
                     KKVDFTSPYYQSGLSYLVRKADAEKIKTFADLKGKTLAVQIGTTGAAYAKNVEGARVS
                     AFNTTSEAFMDLNVKNADAVVLDRPVLAYFLKTKPRVAKNLQLSTEIADAEHFGFAVK
                     KGNAELLQKLNAALEELKKSGEFAKIEDKWFAK"
     gene            complement(7650..8348)
                     /locus_tag="ACZPB6_04875"
     CDS             complement(7650..8348)
                     /locus_tag="ACZPB6_04875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1183838.1"
                     /translation="MTAFRPVPATGFMPLAAVFDLDHTLIAGDATIGWTEYLYERGVV
                     TDPVFREINERMKASYHAGTLDIAAWLREAVPAYSGLDEAERNALVSDFIEEKIRPIA
                     YPQGFETIRAAKAAGMLTLIISASNAFFVKPIAERVFGVDVALGTELVVRNGRITPEL
                     SGTPTFREGKVVRLCAALEHRGLRPAETVFYTDSRNDLPLALASGDCVAVNPDPTLLE
                     AARENGWLVNAWKL"
BASE COUNT         1520 a         2250 c         2966 g         1702 t
ORIGIN      
        1 tcgaacctac gaatgtcgga ttcagaatcc gatgccttaa ccggacttgg cgatacccca
       61 atcaatctgg tgcggtcgga gagactcgaa ctctcatggc tctcaccaca gcgacctcaa
      121 cgctgcgcgt ctaccaattt cgccacgacc gcgaaacctt ctcagcttgt atttggtgct
      181 acgcaccgga cgcgtcgtca ttgctgagcc tgacggcaca tccaacagaa cggttggcag
      241 gggtagtagg attcgaacct acgaatgtcg gattcagaat ccgatgcctt aaccggactt
      301 ggcgataccc cagtaaatgg tgcggtcgga gagactcgaa ctctcatggc tctcaccaca
      361 gcgacctcaa cgctgcgcgt ctaccaattt cgccacgacc gcggcccgaa ctgttcccca
      421 ctgatgtgca tccgaagaag cgaggtcacc agcagaggag gtgtatttta cctcggaatt
      481 tcgttcgtgg gggccttatt agggttttca ctaccagaca taccatattc atgggatgtc
      541 ggctgttcga ccgttgacaa aacaccaccc gctggctgag cggaacgctt gttgaaggcg
      601 ccggaagcga gcgtcagggc gatcgtggag cagaagaaga ccgtggcggc gatggccgtg
      661 gaacgggaga ggaagtttgc gctgcccgta gcgccgaaga cagagccgga ggcgccggaa
      721 ccgaaggcgg caccgaggtc ggcacccttg ccgtgctgca tgagcacgag cacgatcacg
      781 acgacggcag agatgatctg cagaacgaga gcgatgctaa gaagaaaatg catggcggtt
      841 aaaccttaga gtgaaaaatc aatcgacgcg ggcagccgcg cagacggaat ggaaatcttc
      901 agccttgagg gcggcgccgc cgatgaggcc gccgtcgatg tcgggctggg agaagagttc
      961 atgggcattg tccggcttga tgctgccgcc gtagagcagc ggcgtcttcg cggcggcttc
     1021 ggcgtcggcc tcggtgaggc aggcgcgcag agccgcgtga acgtgctggg cggcctgcgg
     1081 agtcgccgtg cgtcccgtgc cgatggccca gacgggttca taggcgacgg cgccgagctt
     1141 ggcaacgggc actcgcgtga gcacggttcg aagctggcgc agcacgacgg cctcggtgag
     1201 gcctgcctcg cgctcggcga gcgtctcgcc cacgcagacg acgggggcga tgccgtgagc
     1261 aacgaggttt tcagcttgga cggccacgga ttcgtcgtcc tcaccccaga gggtgcggcg
     1321 ttccgaatgg ccgacgaggc agaggctcat gccgatgtcg gcgagcatgg cggcggacac
     1381 ttcgcccgtg aaggcgccct gatccctggg cgaaaccgtc tgggcgccga cagcgacccc
     1441 cgtgtccttg aagaggcgga cgagttcgga cagatagacg aagggcgcgc acacggcggt
     1501 cctgcagttg agatcgccga gtttggcaag gaattcctcc gcccagaggc ggttggcctc
     1561 ggcggagccg ttcattttcc agttggcgat gacaatcttt tcacgcacgc tgagtttctc
     1621 caacaatgcc ggtgaaggat gcggatggtc cgtttttgac gcggacaatg cataaccctc
     1681 gcattttata cggtttccct caagtcttca aagcactcgg ggaaaaaacg atggggtttt
     1741 gaaacaagaa gccctgctct cctcgcggag agcagggctc gtgtcttcaa gcccggaagg
     1801 gctcagggat taggcctggg cggcttcgtc cttgagaaga gccttcatgg aaaggcgcac
     1861 gcggcccttt tcgtcggctt cgatgacctt gacgcggacc tgctggcctt ccttgaggta
     1921 gtcggagacc tggttgacgc gttcgttggc gatctggctg atgtgaagga ggccgtcctt
     1981 gcccggcagg agctggacga tggcgccgaa gtcgagcagg cgctggaccg tgccttcgta
     2041 gatctggccg acttcgatct tggccgtgat gatctcgatg cggcggcggg cttcttcaac
     2101 gcgggccgtg tccgggctgg cgatggtgac cgtgccgtcg tcttcgatgt tgatcgtcgt
     2161 gccggtttct tcggtgaggg cgcggatggt ggcgccgccc ttgccgatga cttcacggat
     2221 cttgtcctga tcgatcttga aggagatcat gcgcggggcg aagtcggaga gttccttggc
     2281 gccgccgccg gccatttcat gcatcttgcc gaggatgtgc tggcggcctt cgtgggcctg
     2341 ggccagggcg gcctgcatga tttcggccgt gatgccttcg atcttgatgt ccatctggag
     2401 ggcggtcacg ccttcggcgg taccggccac cttgaagtcc atgtcgccga gatggtcttc
     2461 gtcaccgaga atgtcggtga ggacggcgaa cttgttgcct tccttgatga ggcccatggc
     2521 cacgccggcg acgtagtcct tgagcggaac gccggcgtcg agcatggaga ggcagccgcc
     2581 gcagacggag gccatcgagg aggagccgtt ggattcggtg atttcagaaa cgacgcggat
     2641 cgtgtactgg aattcctcgg cggtcgggag aacggccttc agggcgcgct tggcgagacg
     2701 gccgtggccg atttcgcggc gcttcgggct gccgacgcgg cccgtttcgc ccgtggcgaa
     2761 cgggggcatg ttgtagtgca gcatgaagcg gtcgtgcgtt tcttcgcaga gaccgtcgat
     2821 gatctgttcg tcctgcttcg tgccgagcgt cgtaagcacg agggcctgcg tttcgccgcg
     2881 ggtgaagagg gcggagccat gggtgcgcgg aagcacgctc tggcggattt cgatcgggcg
     2941 gaccgtgcgg gtgtcgcggc cgtcgatgcg gggttcgccg ttgaggatgt tggagcgaac
     3001 gagcttggct tcgagttcga agaggatgcc gtggacttcg ttcatgtccg gagcttcggt
     3061 gccggcggct tcggcgtcgg cgacgagctg ggcttcgcag gcggcataga cttcgcggag
     3121 cttttccgta cgagcctgct tggagcggat gccgtaggct tccttgaggc cggcgtcgca
     3181 gagttcgacg atgcgggcga cgagggcttc gttcttcggg gcgggctgcc agtcccaggc
     3241 cggcttgccg gcttcggcga cgagctcgtt gatggcctgg atgcagacct gcatggcctt
     3301 gtggccggcc atcacggctt cgagcatcgt cttttccgga aggatgtcgg cctcggattc
     3361 aaccatgagg acggcgcgtt ccgtaccggc gacgacgagg tcgaggcggg aggtcttgag
     3421 ttcttccgtg gtcgggttga tgacgatttc gtcattgatg tagccgacgc ggcaggcgcc
     3481 gatcgggccg cggaacggga tgccggagat ggcgagggcg gcggaggcgc cgatcatggc
     3541 cggaatgtcc ggatcaacgg cggggtcggc ggagaggacg tggatgataa cctggacttc
     3601 gttgaagaag ccgtccggga agagcgggcg gatcggacgg tcgatcaggc gggaggtgag
     3661 cgtttccttt tcggacggac ggccttcgcg cttgaagaag ccgccgggga tcttgccggc
     3721 agcgtaggtc ttttcgatgt agtcgacggt gagcgggaag aagtcttggc cgggcttcgt
     3781 ttccttcttg gccacgaccg tggcgagcac caccgtgtcg tccatctggc agatgcaggc
     3841 gccggtggcc tgacgggcga tctcaccggt ggtgagggtc acgtcgtgat tgccgaagcg
     3901 gaaggacttg gacactttgt tgaacatcgt catgatgtat tcctctgtgc atggttgtcg
     3961 tcgctgacgt tactcatcaa ctttccgggc gcaagagcaa ttcgagttcg aagctctttg
     4021 gcacgctgcg gcatcagatc ccttttccct tgtgccggag ggggagggag ctcatgtcgc
     4081 aacgcggtag cctcgcgtgg aaagaaagtg tgccgtcaac gaacgacgtg ggttttagat
     4141 gcgacgacgg cctgtcgtcg ttcggcaggc cgttgtcagg gagaaatgtt ccgtcttact
     4201 tacggagatt gagggcttca atcagggcgc ggtaagcatt gaggtcggtg cgcttgaggt
     4261 agtcaagcag cttgcggcgg cgggagacca tacggagaag gccacgacgg gagtggtaat
     4321 ccttggtgtg aaccttgaag tggtccgtaa ggtggttgat gcgggcggtg agaagagcaa
     4381 cctgcacttc gggagagccg gtgtcgcctt cagcgcgctg gaacttggca acgatctcag
     4441 acttgttcat ttcaacggac atttgttttc ctgaataaag aaaaaggtat ggaaacttgc
     4501 ggacacgagc gttgagactc gagccgtgaa agcggagatt gtacctactg catgggcgcc
     4561 gtgcaagtgc aaagcgagga aattttcaca caaggcggcg cgggctccga aacggcctcc
     4621 gcgggttgac tgtcaagggc cccggaagcc ctgaaaaagg tatgaaaaac gccgcgctcc
     4681 cgtggggatg cgcggcgttc ggaaggctct tgggatgtca gcgctcgtcg ctcagacggt
     4741 agcgcttttc aagccagccg acgagctggc tgatggtgag cgtcatgaca aggtagagga
     4801 tcgcgacgca ggtccagatc tccatcgtgc cgtaggtttc ggcaatgacg agctggccgg
     4861 agcgggtcag ttcctcgaag ccgatcacgg agacgaggga ggagtccttc agcatggcga
     4921 tgaactcgtt gccgagcggc gggatgatgc gcttgacggc ctgcggcagc acgatgtagc
     4981 gcatcgtctg cgggtaggtc atgccgaggg agaggccggc gcgcatctgg ccgagcgaga
     5041 tggactggat gcccgcacgg aagatttcgg cgatgtaggc gcccgagttg agcgagcagg
     5101 ccgcgacggc ggcaatgtag gggtcgatgc gctcaccgat gatgttcggc agggcgaagt
     5161 agatgatgaa gatctggatc agaagcggcg tgccgcggat cacgtcgacg tagactttgg
     5221 cgggccagcg gaagatggga ttcttcgaaa gctgggcaag accgaggaaa aggccgatga
     5281 tcaggccgag acccacggcg agcgccgtga tttcaagcgt caggcccgcg cccttgagaa
     5341 gcaggggaag gctgttgacg atcagggata aatcaaattc catgacgggt gacctctaag
     5401 cagattgtgg tttgcgtccg cttccggatg aagggcgggg gggatgctct tcgaaggggg
     5461 agggcccggg cgcggcgcaa tgataaaagt gtaaggcaaa atttgctgaa ttttacagac
     5521 acggatcaag gttgtctaac cgtacagggc aaaccttgag gcgtctcaaa gaaaaagccg
     5581 gcgcgctccc ttgcgggaga ggccggactc ctgtgtcgtc agatcacgga tttgcgaaaa
     5641 atcagttctt gccgaaccac ttgttgtaga tcttgtcgta ttcgccgttc gacttcagct
     5701 tggcgagcgc agcgttgacc tcgtccttga gtgcggactg gtccttgcgg aagacgaagg
     5761 caaagtcgtc ggcggagtag gtttcgggct ggtgcacgag ttgggcgccg agcttgggct
     5821 gttgcgtcat gatgtaggcc gtcacgggac ggtcgttgat tacggcgtcg ctgccgccga
     5881 tggagaggtc gagaatggcc tctcccgcgg tgttgaacgt cacgatcttg gcgcccttga
     5941 cctttttggc ggcgttgtgg ctcgtcgtgc cgatctgcac gccgatcgtc ttgccctcaa
     6001 ggtccttcat gctcttgacg gcgctggctt tggccttcgt ggtgacgagg gagaggccgg
     6061 aagtgtagaa cgtgtcggag tagaggacgc gcttcttgcg ctcctcggtg atggagaagc
     6121 ctgcggcgcc catctcgacg gtgcccgaga gcatggcggg gatgatggcg tcgaagctga
     6181 ggacctggaa ttcgattttg gcgccgagtt ccttgccgac ggcccgcacg aggtccattt
     6241 cgaagccgga gacttctccc gtctgggtgt tgacgaattc aaaggggggg aacgtcggtt
     6301 ccgtggccac cttgatgagg cgttccgcaa acgcgttggc ggacatgccg ccgagaacgg
     6361 cggcgctcat gatgacgccg gcgaacgtct tgacgccggc acgtgcgagc ttgaattgca
     6421 tttgtaaggc cctcctgggc ttgtagcggg agcgcggtgc tccctgtttt aataccgttc
     6481 gcgtgaacgg cccgagagtt cctgggctgc agggggatgc ggcttcggac gttggaaatt
     6541 cttttcaggc ggcctgtttc aggttgtcgg actgcgggtg atgtgctgtt tttggtcagt
     6601 ccggcccgcg cgccgcgcgg ctggcgcggc acggggactt tggtgaggag aggttacttg
     6661 gcgaaccact tgtcctcgat cttggcgaat tcgccggact tcttgagctc ttcaagggcg
     6721 gcgttgagct tctggaggag ctcggcgttg cccttcttca cggcgaagcc gaagtgttcg
     6781 gcgtcggcga tctcggtgga gagctggagg ttcttggcaa cgcgcggctt cgtcttcagg
     6841 aagtaggcga gcaccggacg gtcaagcacg acggcgtcgg cgttcttgac gttgaggtcc
     6901 atgaaggcct cgctcgtcgt gttgaaggcg gagacgcggg cgccctcgac gttcttggcg
     6961 taggcggcgc ccgtcgtgcc gatctgcacg gcgagggtct tgcccttgag gtcggcgaag
     7021 gtcttgatct tttcagcatc ggccttgcgg acgagatagg agaggccgga ctggtagtag
     7081 ggggaggtga agtcgacctt cttctgacgt tcttcggtga tggagatgcc cgaggcgatc
     7141 acgtcgatcg tgccggcgga gagcgccggg atgagggcgt caaagcccat gttcatgacc
     7201 ttgacgtcgt agccggcctt gtcggcgacg gccttgatga ggtcgatgtc gaagccggag
     7261 aactcctggg tcttcgtgtc gaggaattcg aacggcgcga acgtgggttc ggtgccgacg
     7321 cgcaacgtgt cgcgggccat ggcgttgccg gcgaaggcgg cgatggcaag ggcggaggcg
     7381 gcagcggcga aaactttctt gaacatcatg gtaaggcggt cctttgagat tctgtctgtt
     7441 cgttttgcgg atccggagcg cctcctgcaa ggcgcttcgt ccgtaggatg caatatagcc
     7501 gaagtgttcg gccccctgcg agacgaatcg cccaagtttg tacgccgctc agaccgtata
     7561 ggtcctatac cccaatattc gcgcgccttc gtatcaaagc cgcccaattg tcggcaaaag
     7621 gcgggggcgg aagaagcctc aagggcgtct caaagcttcc atgcgttcac cagccatccg
     7681 ttctcgcgag cggcctcgag cagcgtgggg tcggggttga ccgccacgca gtcgcccgag
     7741 gcgagcgcga gcggcaggtc gttgcggctg tcggtgtaga agaccgtctc ggcggggcgc
     7801 agccccctgt gctcgagtgc ggcgcaaagg cgcacgacct tgccctcgcg gaaggtgggc
     7861 gtgcccgaga gctcgggcgt gatgcggccg ttcctgacga cgagctcggt gccgagcgcg
     7921 acgtcgacgc cgaagacgcg ttcggcgatg ggcttcacga agaaggcgtt ggaggcggag
     7981 atgatgagcg taagcatgcc cgcggccttc gcggcgcgga tcgtctcaaa gccctgcgga
     8041 taggcgatgg gacggatctt ctcctcgatg aagtcggaga cgagcgcatt gcgctcggcc
     8101 tcgtcaagtc ccgagtaggc gggaaccgcc tcgcgcagcc aggcggcgat gtcgagcgtg
     8161 cccgcgtggt aggaggcctt catgcgttcg ttgatctcgc ggaagacggg atcggttacg
     8221 acgccccttt catagagata ctcggtccag ccgatcgtgg catcgcccgc gatgagcgtg
     8281 tggtcgaggt cgaacacggc ggcaagcggc atgaagccgg tggcgggaac ggggcggaag
     8341 gcggtcatgg cggggagagt ccttggagtg cttgcaggga tcgtcattct agtcgttctc
     8401 atccaaatgt gatgaaagcc gcaccgggct tttctcgt
//
LOCUS       DBMDTI010000071         8293 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2342_length_8293_cov_4.485474, whole genome shotgun sequence.
ACCESSION   DBMDTI010000071 DBMDTI010000000
VERSION     DBMDTI010000071.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2342_length_8293_cov_4.485474"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="ACZPB6_04880"
     CDS             <1..323
                     /locus_tag="ACZPB6_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183839.1"
                     /translation="AFFTMPARIQADPVLWTPDWTAAHASAREHVGELPWTAGNFKDR
                     LRQGLENWWNNLDASELKLPEFNTEDFSLPEFRLPDIELPDWANEFIGKEKAPENSDA
                     RNPV"
     gene            complement(330..608)
                     /locus_tag="ACZPB6_04885"
     CDS             complement(330..608)
                     /locus_tag="ACZPB6_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HIR1183840.1"
                     /translation="MITDECISCGVCLPECPNEAIVEDNGVFRIRGGRCTECAGFFDE
                     PKCRELCPVSECIVPNPRRPETREMLLEKHRRLELRKRVEAAMKVTGL"
     gene            complement(621..1112)
                     /gene="coaD"
                     /locus_tag="ACZPB6_04890"
     CDS             complement(621..1112)
                     /gene="coaD"
                     /locus_tag="ACZPB6_04890"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR1183841.1"
                     /translation="MTTAVYPGTFDPMTNGHVDLVKRASRIFDHVVVGVAESRKKRPM
                     LSLETRVALARGICREFENVTVQPFDGLLKDFVAACGAGVIIRGARAVSDFEYEFQMA
                     GMNRQLMPDVETIFLTPSDQYQFVSGTFVREIAQLSPKDAARFVTPEVLEALIEACAD
                     KKE"
     gene            complement(1109..1708)
                     /locus_tag="ACZPB6_04895"
     CDS             complement(1109..1708)
                     /locus_tag="ACZPB6_04895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015559.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HIR1183842.1"
                     /translation="MSGKWKRSVVPVACAEGLRPTSERVRETVFDWLTHLFGTLEGCD
                     ALDMFAGSGAMGLEAASRGADYVDWLDIHRQGIAGIRQALTRLGADDHKQARVADAFR
                     FLQDAPRSYDVIFIDPPFSLDMQVKAARAAFGALKEEGLLYVESPTELLSDEALESLG
                     VVRVRSGSAGAVRFELLAKAGSGMAGLVRLSKEEKRSRK"
     gene            1811..2761
                     /gene="ftsY"
                     /locus_tag="ACZPB6_04900"
     CDS             1811..2761
                     /gene="ftsY"
                     /locus_tag="ACZPB6_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR1183843.1"
                     /translation="MTGSSEGWFSRLKNGLARTRVNIVGLFSGGVIDDDFLEELEYAL
                     ITADVGVQASTRILQRLRDTIKLKGLKTQDEVRLALRDEIARMLAPAQRELDIEQAHP
                     LVIMMTGVNGAGKTTSIGKISKYLSQRGKSVLLAAGDTFRAAAREQLAVWGDRNQIEV
                     IAQQGGDPAAVVFDAVSAGKARGTDVVIADTAGRLPTQINLMEELRRIRRAQDKAMTG
                     APHEAILVVDGTNGQNALTQVKAFDAAVGLTGLIVTKLDGTAKGGVLVAITQMRPENP
                     LPIYFIGVGEAIDDLQPFNAVQFANALVGLDPNTPATAPE"
     gene            2786..4255
                     /locus_tag="ACZPB6_04905"
     CDS             2786..4255
                     /locus_tag="ACZPB6_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR1183844.1"
                     /translation="MLLGAVDLGSNSFRVEIGRVDGDRIVTQSYWKETIRMAAGFDKN
                     GALTPEMQEKALAALSRFHEKLAGLPATQVRAVGTQALREAANSSEFLPKAEAALGYP
                     IGILSGHEEARLVFKGCARTLPPSDKRRLVVDIGGASTEFVVGQDLEAQEYESYHVGC
                     VNTSMKFFADGVITAEKLDKAIVACCAEFSEGEHKFNDTKFDEAYGSAGTFGAVSDIC
                     VAQGWSDGEVTPEHLEKIRALLLRAGNINNIQFPGLKPDRREVIAGGYAVLTAVYRTA
                     GIKSMRPASGALRVGLLYEQLGIVQNRDTRDISVDAIIRTCGVSREQADIVAGYAVEF
                     LRMLDPGIDPAALKRLRWAALMHEVGTTISTSRYHRHGEYIVSNADLPGFTRTDQQYM
                     AKLILAQRGGLGKAEDFLKSQANTRQVLAMRLAVIFAHARKPAPLPLITITRSLNNSV
                     LNIAIDRTWLEQHPLTAYMLQQEADDWKKVGRVITVTTF"
     gene            4264..4941
                     /locus_tag="ACZPB6_04910"
     CDS             4264..4941
                     /locus_tag="ACZPB6_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR1183845.1"
                     /translation="MSETEAPLLQLNGVSVVYDGHAVIDDLSLEVGRGEFVVIHGSTG
                     CGKSTILRLFAGLIDPTKGEVVVAGDRIDEFDEAQRRWLRRSMGLMMQEELLLEDRTV
                     LENVMLPALAADETAQEARRRALLALNKCGAADLADLRPSSLSAGQRQIACLARAVVN
                     RPVLILADEPATHLDKTNAQTLINLLSAFALAGVTVVAATHQHLTPEGCRSREITLSA
                     SNRGGAA"
     gene            4938..5828
                     /locus_tag="ACZPB6_04915"
     CDS             4938..5828
                     /locus_tag="ACZPB6_04915"
                     /inference="COORDINATES: protein motif:HMM:NF037183.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR1183846.1"
                     /translation="MSYASSRAWALKETFRGFRQNFSLFSLATFLSALALSIPLFIST
                     IFYELAEPIRQIPTSVEITVFTKSGTDMEKLSKAVAEIETIERVSLVPKDEAIRSLNE
                     HLGLKDKAVKHNPLPDILIATVSSSTSPAGVDAAAGRIEKIEGVDMLAYETDWREKLD
                     ALSNAARLGLLCLGIVVLTLVMLVVAASIRLTTLSVQPQMKALYLFGASPSFAMRPIA
                     WRGFILMMSASLGAIAITYAGLAVLGHPIATVARLYEVELTLALPPVDWCAGFVIICS
                     LIGYIIALLSAQDAWRKARR"
     gene            5932..6858
                     /gene="rpoH"
                     /locus_tag="ACZPB6_04920"
     CDS             5932..6858
                     /gene="rpoH"
                     /locus_tag="ACZPB6_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HIR1183847.1"
                     /translation="MTEKPKGTALVAYKAPSVPALVGPLGDLEAFMHAAERAPILTPE
                     EERSLAVRLRDDGDVEAAQRLILSHLRLVISIARGFLGYGLPYADLIQEGNIGLMKAI
                     RHYDPDRGARLMTFAVHWIRSEIQEYIIRNWRIVKLATTKNQRKLFFNLRQMKEDPQT
                     ALTNSQAEHIALALDVKPKEVLEMEERLYGQETSLDTPSNADDDSDNAFTPSDWLTRE
                     SDEPEAILEEKEHQRLEGEGLQMALRSLDERSRRIITARYLNLDKEGNSKAVTLQELA
                     NEFGVSAERIRQIEKLAIQKMRQTLEQAHYSE"
     gene            6867..7337
                     /gene="trmL"
                     /locus_tag="ACZPB6_04925"
     CDS             6867..7337
                     /gene="trmL"
                     /locus_tag="ACZPB6_04925"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HIR1183848.1"
                     /translation="MFNVVLVHPEIPPNTGNVIRLCANTGCTLHLVKPLGFSLDDKHM
                     KRAGLDYHEYATIRIHESWEAFLESEHPDVSCMYAMTTHGSHPFADSSFTPGDWFVFG
                     CEGSGLPEGIRNSFPEAQRIRLPMRPKNRSLNLSNAVAVTVFEAWRQNGYDGGI"
     gene            complement(7429..8091)
                     /locus_tag="ACZPB6_04930"
     CDS             complement(7429..8091)
                     /locus_tag="ACZPB6_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1183849.1"
                     /translation="MTQSIRIILVDDHTLFRSGVKALLQRQPDFEVIGEASDGLEGVK
                     LVEQLDPDVVLLDVDMPSMNGCEAMAQIHESHPGLAVLMLTVSEDCETLAECLRLGAR
                     GYLLKKIDQDYLLRAIRSAYNGESIIAPQMMTKFVNRFADPEPRNAQDMEMTALTRRE
                     RQTLAWLARGVSNKEIARAMNLAESTVKVHVQSCLRKLGLSSRVQAAIYAVEHGLDKE
                     LD"
BASE COUNT         1650 a         2874 c         2302 g         1467 t
ORIGIN      
        1 ccgccttctt cacgatgccc gcccgcatcc aggccgaccc cgtcctctgg acgccagact
       61 ggacggccgc ccacgcctcc gcccgcgaac acgtcggcga gttgccctgg acggccggca
      121 acttcaagga ccgcctccgt cagggacttg aaaactggtg gaacaacctc gatgcgtccg
      181 agctgaagct ccccgaattc aacaccgagg acttcagcct ccccgagttc aggctgcccg
      241 acatcgagct tcccgactgg gcgaatgaat tcatcggcaa agaaaaggcg ccggaaaact
      301 ccgacgctcg caaccccgtc tgacgaacgt tacaagcccg tcaccttcat cgccgcctcc
      361 acccgctttc tgagctcgag gcggcgatgc ttttcaagca gcatctccct tgtttcagga
      421 cggcggggat tgggcacgat gcactcgctc acagggcaaa gctccctgca cttgggctcg
      481 tcaaagaagc ccgcgcactc cgtgcagcgg ccgccgcgga tgcgaaagac gccgttgtcc
      541 tccacaatgg cctcgttggg gcattccggc aggcagacgc cgcaggaaat gcattcgtca
      601 gtaatcatca gcgccatcat ctactccttc ttgtcagcgc atgcctcaat cagtgcctcg
      661 agcacctcgg gcgtcacgaa gcgggccgca tccttcggcg agagctgtgc aatctccctg
      721 acgaaggtcc cactcacgaa ctggtactga tccgaaggcg tcagaaagat cgtctcgacg
      781 tccggcatca gctgccggtt catgccagcc atttgaaatt catactcaaa gtcactcacg
      841 gccctcgcgc cgcgaataat cacgcctgca ccgcaggcgg cgacgaagtc cttgagcaac
      901 ccgtcgaagg gctgcaccgt cacgttttcg aactccctgc agatcccgcg ggcaagagcc
      961 acgcgggttt cgagcgaaag catcggacgc ttcttgcgac tttccgcaac gcccaccacc
     1021 acatgatcga agatgcggga ggcgcgcttc acgagatcaa cgtgcccgtt cgtcatggga
     1081 tcgaacgtgc ccggatagac tgccgtcgtc atttcctgct cctcttctct tccttggaca
     1141 ggcgtacgag ccccgccatg ccgcttcccg ctttggcaag cagctcgaag cgcacggcgc
     1201 ccgcactgcc cgagcgcacg cggacgacgc cgagggactc gagcgcctcg tcggagagca
     1261 gctccgtcgg cgactccacg tagagaaggc cctcttcctt gagcgccccg aacgcggcgc
     1321 gtgcggcctt cacctgcatg tcgagactga agggcggatc gatgaagatc acgtcatagc
     1381 ttcggggcgc gtcctgcaga aagcggaagg catcggccac gcgcgcctgc ttgtgatcgt
     1441 cggcaccgag tcgcgtgagc gcctggcgga ttcccgcaat gccctgacga tggatatcaa
     1501 gccagtcgac gtaatccgca cccctgctcg cggcctccag ccccatggcg ccggaaccgg
     1561 caaacatgtc gagcgcatcg catccctcga gggttccgaa gaggtgcgtg agccagtcga
     1621 agacggtctc cctcacccgc tcggaagtgg ggcgaagccc ttcggcacaa gcaacgggaa
     1681 cgacgctgcg cttccacttg cctgacacaa ttcgtacgct tcccgctccc cgaacgggcg
     1741 cgggcgttgc taaacttgag ttacttgcgc ccaggcgcct tgaattcttt cgaactgcca
     1801 ttagagaaat atgactggat cttccgaggg atggttttcc cgattaaaaa atggtcttgc
     1861 cagaacgcgc gtcaatatcg tcggcctgtt ttcgggcggc gtgatcgacg acgactttct
     1921 tgaagagctc gaatacgcac tgatcacggc cgacgtcggc gtgcaggcgt cgactcgtat
     1981 cctacaacgt ttgcgcgaca ccattaagct caagggcctc aaaacgcagg acgaagttcg
     2041 ccttgcactt cgagatgaaa tcgcccgcat gctcgcgccg gcgcagcgcg agctcgacat
     2101 cgagcaggcg catcccctcg tcatcatgat gacgggcgtc aacggcgcag gcaagaccac
     2161 ctccatcggc aagatctcca agtacctgag ccagcgcggc aagagcgtcc tactcgccgc
     2221 aggcgacacg ttccgagccg ccgcccgcga acagctcgcc gtctggggcg accgcaacca
     2281 gatcgaagtc atcgcccagc agggcggcga tcctgccgca gtcgtcttcg acgccgtgag
     2341 cgcaggcaag gcgcgcggca ccgacgtcgt catcgccgac accgccggcc gcctgcccac
     2401 ccagatcaac ctcatggaag agttgcgccg aatccgccgc gcccaggaca aggccatgac
     2461 cggcgctccg cacgaggcga ttctcgtcgt cgacggcacc aatggtcaga acgcgcttac
     2521 ccaggtgaag gccttcgacg ccgcggtcgg cctgaccggc ctcatcgtca cgaaactcga
     2581 cggcaccgcc aagggcggcg tgctcgtcgc cattacacaa atgcgtcccg aaaatcccct
     2641 gccgatctac ttcatcggcg tgggcgaagc catcgacgac ctgcagccct tcaatgcggt
     2701 gcagttcgcc aatgcgctcg tcggactcga tcccaacact cccgctacag ccccggaata
     2761 atcaattaaa aatggagcac acaagatgct tttaggtgca gttgatcttg gaagcaacag
     2821 cttccgcgta gagatcggcc gtgttgacgg cgaccgcatc gtcactcaga gctactggaa
     2881 ggaaaccatt cgcatggccg cgggcttcga caagaacgga gccctcacgc ccgagatgca
     2941 ggagaaggca ctcgccgcac tgagccgctt ccacgaaaag ctcgcaggcc ttcccgccac
     3001 ccaggtgcgt gccgtcggca cgcaggcgtt gcgcgaggcc gcgaactcct ccgaattcct
     3061 tcccaaggct gaagctgcgc tcggctatcc catcggcatt ctgagcggcc acgaagaggc
     3121 ccgcctcgtc ttcaagggct gtgcacgcac gttgcccccg tccgacaagc gccgtctcgt
     3181 cgtcgacatc ggcggcgcga gtacggagtt cgtcgtcggc caggaccttg aggcccagga
     3241 atacgagagc taccacgtcg gctgcgtgaa cacgtcgatg aagttcttcg ccgacggcgt
     3301 catcaccgcc gaaaagctcg acaaggcaat cgtcgcctgc tgcgccgagt tcagcgaagg
     3361 cgagcacaag ttcaacgaca ccaagttcga cgaagcctac ggctccgccg gcaccttcgg
     3421 cgccgtttcc gacatctgcg ttgcgcaggg ctggagcgac ggcgaagtca cgcccgagca
     3481 tcttgaaaag atccgcgcgc ttctgcttcg cgccgggaac atcaacaaca tccagttccc
     3541 cggcctcaag cccgaccgcc gcgaagtcat cgctggcggc tatgccgtcc tcaccgccgt
     3601 ctaccgcacc gcaggcatca agtccatgcg tcccgccagc ggcgcccttc gcgtgggtct
     3661 tctctacgaa cagctcggca tcgtccagaa ccgcgacacc cgcgacatca gcgtcgacgc
     3721 gatcatccgc acctgcggcg tgtcccgaga gcaagccgac atcgtcgcag gctacgccgt
     3781 tgaattcctc cgcatgctcg accccggcat cgatcccgcc gccctcaagc gcctgcgctg
     3841 ggccgcgctc atgcatgaag tcggcacgac gatctcgacg agccgctacc accgccacgg
     3901 cgaatacatc gtgagcaatg cggacctgcc cggattcacg cgcaccgacc agcagtacat
     3961 ggctaagctg atcctcgccc agcgaggcgg cctcggcaag gccgaagact tcctcaagtc
     4021 ccaagccaac acccgtcagg tgctcgccat gcgccttgcc gtcatcttcg cgcatgcccg
     4081 caagcccgcg ccgcttccgc tcatcacgat cacccgcagc ctcaacaact cggtgctcaa
     4141 catcgccatc gaccgcacct ggcttgaaca gcatcccctc accgcctaca tgcttcagca
     4201 ggaggcggat gactggaaga aggtcggccg cgtgatcacc gtgaccacct tctgagaggt
     4261 ctcatgtccg aaaccgaagc acccctcctg cagctgaacg gcgtgagcgt cgtctacgac
     4321 ggtcatgccg tcatcgacga cctcagtctt gaagtcggac gcggcgaatt cgtcgtcatt
     4381 cacggcagca cggggtgcgg caaaagcacc atcctcaggc tcttcgccgg tctcatcgac
     4441 ccgacgaagg gcgaagtcgt cgttgcggga gaccgcattg acgagttcga cgaggcccag
     4501 cgccgatggc tcaggcgctc catgggcctc atgatgcagg aggagcttct acttgaggac
     4561 aggaccgttc tcgaaaacgt catgttgcct gcgctcgcag cggacgaaac ggcacaggaa
     4621 gcgcgccgca gggcgcttct ggccctcaac aagtgcggcg ccgctgatct cgccgacctt
     4681 cgtccctcga gcctctcagc tggccaacgc cagatcgcct gtctggcccg cgccgtcgtc
     4741 aaccgtcccg tgctcatcct tgccgacgag cccgcgacgc acctcgacaa gacgaacgcc
     4801 cagacgctca tcaaccttct gagcgccttt gccctcgcgg gcgtcaccgt cgtcgccgcc
     4861 acgcaccagc atctgacgcc ggagggctgc cgttcgcgcg aaatcacgct ctccgcgtcc
     4921 aacaggggag gcgccgcatg agctacgcct cctcgcgcgc ctgggcgctc aaggaaacct
     4981 tcaggggctt ccgccagaac ttcagcctct tctcgctcgc gaccttcctg tcggcgcttg
     5041 cgctttccat tccgctcttc atctcgacga tcttctacga gctcgccgag cccatcaggc
     5101 agattccgac ttccgtcgaa atcaccgtct tcacgaagtc gggcacggac atggagaagc
     5161 tctccaaagc cgtggccgaa atcgaaacca tcgagcgcgt ctcgctcgtg cccaaggacg
     5221 aggccatccg aagcctgaat gaacatctcg ggctcaagga caaggcggtc aagcacaatc
     5281 cccttcccga catcctgatc gccaccgtca gcagcagcac ctcgcccgca ggcgtcgacg
     5341 ccgccgcagg ccgcatcgaa aagatcgagg gcgtggacat gctcgcctac gagaccgact
     5401 ggcgcgaaaa gctcgacgcg ctctccaacg cggcgcgtct tggcctcctc tgcctcggca
     5461 tcgtcgtgct cacgctcgtc atgctggttg tcgccgcctc catcaggctc acgactcttt
     5521 ccgttcagcc gcagatgaag gccctctatc ttttcggcgc ctctccgtcc ttcgccatgc
     5581 gtccgatcgc ctggcgcggc ttcatcctga tgatgtcggc aagcctcggc gccattgcca
     5641 tcacgtacgc aggcctcgca gtcctcggcc acccgatagc caccgtcgcc cgcctctacg
     5701 aggtcgagct cacgcttgcg cttcctcccg tcgactggtg cgctggcttc gtgatcatct
     5761 gcagcctgat cggctacatc atcgcccttc tttcggctca ggacgcctgg cgaaaggccc
     5821 gccgctgacc ctgtttcgag agaccgagca tacgtttttt tccggattgg gcgcgccgat
     5881 cggtgctacc atacagtctt ttccgaattt tcggctccga ctcacccaca aatgactgag
     5941 aaacccaaag gcacagccct cgtcgcatac aaggcaccct ccgtaccggc gctcgtcggt
     6001 ccgctcggag acctcgaagc cttcatgcac gcagccgagc gtgctccgat cctgacgccc
     6061 gaggaggaac ggtccctggc cgtccgtctt cgcgacgacg gtgacgtgga ggccgcccag
     6121 cggctcattc tcagccatct ccgcctggtc atctcgatcg cccgaggctt tctcggatac
     6181 ggcctgccct atgcggacct cattcaggaa ggcaacatcg gcctcatgaa ggccatccgc
     6241 cactacgatc cggaccgcgg cgctcgcctg atgacctttg ccgtgcactg gatccgctcg
     6301 gaaattcagg aatacatcat ccgcaactgg cgaatcgtca agctcgccac cacgaagaac
     6361 cagcgcaagc tcttcttcaa tctgcgccag atgaaggagg atcctcagac ggccctcacc
     6421 aacagtcagg ccgaacacat cgcgctcgcg ctcgacgtca agcccaagga ggtcctcgag
     6481 atggaggagc gcctctacgg tcaggagacg agcctcgaca cgccttcgaa cgccgacgac
     6541 gattccgaca acgcgttcac cccgtccgac tggctcacgc gcgaaagcga cgaacccgaa
     6601 gccatcctcg aggaaaagga gcaccagcgc cttgaaggcg aaggcctgca gatggccctg
     6661 cgctcgctcg acgagcgcag ccgccgcatc atcacggccc gctacctgaa tctcgacaag
     6721 gaaggcaaca gcaaggccgt cacgctgcag gaacttgcca acgagttcgg cgtctcggca
     6781 gagcgcatcc gccagatcga gaagctcgcc atccagaaga tgcgtcagac tcttgagcag
     6841 gcccactatt cggaatagtc tccaacatgt tcaacgtcgt tctggttcat cccgaaattc
     6901 cgcccaacac gggcaacgtc atccgcctct gcgccaacac gggctgcacg cttcaccttg
     6961 tgaagccgct cggtttctcc cttgacgaca agcacatgaa gcgcgcgggg ctcgactatc
     7021 acgagtacgc gacgatccgc atccacgaat cgtgggaggc gtttctcgag tccgagcatc
     7081 ccgacgtctc ctgcatgtac gccatgacga cgcacggctc gcatccgttt gcggactcga
     7141 gctttacgcc gggcgactgg ttcgtcttcg gctgcgaagg ctccggcctt cccgaaggca
     7201 tccgcaactc cttccccgag gcgcagcgca tccgcctgcc tatgcggccg aagaaccgca
     7261 gcctcaacct gagcaatgcc gttgccgtca ccgtctttga ggcatggcgt cagaacggct
     7321 acgacggcgg catctgaacc cgccgtccaa gaatcggaca agaaaaaagg gcatcccacg
     7381 cgggcgccct ttctcttgag cggtacagca ggcgtcagcc tgcctgcatc agtcgagttc
     7441 cttgtcgaga ccgtgctcga ccgcgtagat ggcggcctga acacggctcg aaaggcctag
     7501 cttgcgcagg cagctctgaa catggacctt gaccgtgctt tcggcaagat tcatggcgcg
     7561 ggcgatctcc ttgttggaga cgccgcgggc aagccaggcg agcgtttggc gttcgcgacg
     7621 cgtgagcgcc gtcatttcca tgtcctgcgc gttacgcggt tcaggatcgg caaagcggtt
     7681 gacgaacttc gtcatcatct gcggcgcaat gatgctttcg ccgttgtagg cggagcgaat
     7741 ggcgcgaagc aggtaatcct gatcgatctt cttgagcaga taaccgcggg cgcccagacg
     7801 caggcattcc gcgagggtct cgcagtcctc gctcaccgtg agcatcagaa ccgccaggcc
     7861 gggatgggat tcatggatct gggccatggc ctcgcagccg ttcatgctcg gcatgtccac
     7921 gtcaagcaga acaacgtcag gatccagctg ttcgacaagc ttgacgcctt ccagaccgtc
     7981 gctggcctcg ccgatcacct cgaagtcagg ctgcctctga agaagggcct tgacaccgct
     8041 gcgaaacagc gtgtggtcat cgaccaaaat aatgcggatg ctttgagtca tttttttcaa
     8101 tcccgagagc ggctccgact caaatgcggc aaggcggggc tgcttggcaa attcggagtc
     8161 gaacaatttt tgaacagtct ttccattgta ccaacatcac ccgccgcatt ttgcttaaga
     8221 acgcctgctc gccaacacct gaaaaaacga aaggcagccc gaaggctgcc tttcgccggc
     8281 gaatcaaagt acc
//
LOCUS       DBMDTI010000072         8184 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2368_length_8184_cov_3.834591, whole genome shotgun sequence.
ACCESSION   DBMDTI010000072 DBMDTI010000000
VERSION     DBMDTI010000072.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2368_length_8184_cov_3.834591"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="ACZPB6_04935"
     CDS             <1..955
                     /locus_tag="ACZPB6_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HIR1183850.1"
                     /translation="VATDVAARGIDLENLTHVINFGIPASYETYVHRIGRTGRAGRTG
                     TAITILEPREHRHLRAIEGITKARIEVRSVPSATDVRARRLEMMRGSLAEILEAGDLD
                     RFRVVVESLCEEYDPFDVAAAAMKLAQENEGANDDDTEIPAYSPFERPRRERGDKPVR
                     GERAPRRERREAPEEGMTRLFVGAGRDLGIRPSDIVGAIANEAGVPSKCIGAIDITDR
                     FTIVEVDSDRVEQIIDAMEGVRFKGRETTVKLDRPRREPREDRGDFRIEGRGEGRGEY
                     RRERGEDRGWQQRDDSREYRGAKTKRDFRREGGYDKRGPRR"
     gene            1046..1672
                     /gene="pth"
                     /locus_tag="ACZPB6_04940"
     CDS             1046..1672
                     /gene="pth"
                     /locus_tag="ACZPB6_04940"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR1183851.1"
                     /translation="MTEQGIRLIVGLGNPGSEYARTRHNAGFWFVDELARKTGAFFRD
                     ERKFQGEVCRTRIGDSDVWLLKPSTYMNRSGDAVVALALYYKITPDQILVVHDELDLP
                     PGCMKIKQGGGNAGHNGLKDISAKLSTPNFWRLRLGTGHPRTLGMAQQVADFVLSAPS
                     SEHEAAIEDCIGIALKEAGPLAEGDFTGVSRRLAKYGNPPKPPKKAAE"
     gene            1669..2163
                     /locus_tag="ACZPB6_04945"
     CDS             1669..2163
                     /locus_tag="ACZPB6_04945"
                     /inference="COORDINATES: protein motif:HMM:NF016356.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-thiouracil desulfurase family protein"
                     /protein_id="HIR1183852.1"
                     /translation="MKLLVSRCLLGEPCRYDGRVKTDLRAPLRAAGIGDDCWISVCPE
                     SDGGLPTPRTPSEIRGTARGVLEGRDHIMAADGRDNTACFMKGARIAADLCRENGVRL
                     ALLKAKSPSCGFGQVYDGTFTGTLVAGRGLTAELLSDAGVRVLTENDLDLLASLKTQE
                     DPAC"
     gene            2227..3321
                     /gene="ychF"
                     /locus_tag="ACZPB6_04950"
     CDS             2227..3321
                     /gene="ychF"
                     /locus_tag="ACZPB6_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR1183853.1"
                     /translation="MGLKCGIVGLPNVGKSTIFNALTKAGIAAENYPFCTIEPNVGVV
                     EVPDPRLKALSEIVHPERVVPAAVEFVDIAGLVAGASKGEGLGNQFLANIRECDAIAH
                     IVRCFTDDNIVHVSGKVDPLDDINVINTELALADLQTVEKALNRYSKQARSGGDKEAL
                     KLVLVLEKLLPALNEGRAARSVKLTPEEIAVVRPLFLLTIKPTMYVANVEDHGFENNP
                     LLDAVREFAASEHSPVVAVCAKTEADIADLDDEDKEMFLADMGMTEPGLDRVIRAGYD
                     LLGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQTIAYEDFIAFKGE
                     KGAQEAGKMRAEGKDYIVQDGDVMNFLFNV"
     gene            3438..3809
                     /locus_tag="ACZPB6_04955"
     CDS             3438..3809
                     /locus_tag="ACZPB6_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1183854.1"
                     /translation="MATTSTKKRQEIGERLRHERERLGYTKQQIAQLLGVPIPAYECF
                     ENGDDDPGVYRLPRLAACGFDVMFIITAERHVPVAEENELLSRFRDLSQRGRASIFST
                     MDALERLAPNLKQEFRRRFKN"
     gene            3957..4520
                     /gene="ahpC"
                     /locus_tag="ACZPB6_04960"
     CDS             3957..4520
                     /gene="ahpC"
                     /locus_tag="ACZPB6_04960"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HIR1183855.1"
                     /translation="MSLLNTEILPFKAQAYANGEFVELTEASLKGHWSVVFFYPADFT
                     FVCPTELEDLAENYAEFKSLGVEVYAVSTDTHFTHKAWHESSAAVGKVQFPMIGDPTA
                     TLARNFQVLIEADGLAERGTFVINPEGKIVIMEHHDGGIGRDASELLRKVKAAQYVAA
                     NPGQVCPAKWQPGAATLTPSLDLVGKI"
     gene            4609..6159
                     /gene="ahpF"
                     /locus_tag="ACZPB6_04965"
     CDS             4609..6159
                     /gene="ahpF"
                     /locus_tag="ACZPB6_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HIR1183856.1"
                     /translation="MLDANIKAQLQSYFTKLRRPIVLKAALDGSQDATNIEALLKEVA
                     ELSDKISYEVESDASVRRPSFTVAPQGGHEGIRFACLPMGHEFTSFVLALLQTGGHPA
                     KISDEESRQIAALKGPLNFEVFISLSCHNCPEVVQALDLISILNPNATVTVIDGALFR
                     EEAAGRNVMAVPTVFLNGQSFLSGRRELSEILAKLDTSVAEAAQASISAKAPFDVLVV
                     GSGPAGSTAAIYAARKGLRTGIVADRLGGQVNETADIENFTSILKTDGTALAGNFTAH
                     LKSYDIDIITPHSVAKIEREGDMWRLVLENGSELRSKTIIAASGARWKQLEVPGEEEF
                     KGRGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGIASSVVLLQRGSELTADKV
                     LQEKLLSLPNVTVKYRASTSKLTGSNKLEKLFYLNRETGETESIDVDGCFVQIGLKPN
                     TEWLDGAVELNAYGEVAVSDRCTTSAPGLFAAGDCTSVPYKQIVIALGEGAKASLSAF
                     DYLIRQGA"
     gene            6262..>8184
                     /locus_tag="ACZPB6_04970"
     CDS             6262..>8184
                     /locus_tag="ACZPB6_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR1183857.1"
                     /translation="MRYVRSFLLALTTLAFFLLVLVAGLYVFITPERVSARLANTLSH
                     HLGLKIEMAKLHIDTRLPSLKFTVKDAVLTSADGAVRGSVPSATIRLHPLALFTRSPR
                     ISEITTADGSLTFGNTSPEDVRAWIEGTVKPLAFNVDHLVVHRGAVYFSDPLTGPEPW
                     AYVNNASVSISNLSEAGAAYSLNGVIKLPEMLGSAELRGITDWSRGLLEARTEQLDAS
                     FKGEVRGRRTEFAGHADRIALTRENASLSNVAASLKRGEETLLELSSPAVTLDRHTLD
                     TPVLTTALTVMSPEGSLTLSASSALKADFGARRLEAPSITVAASERAAGATTASNAGS
                     LTGSLLWNASSGEGRAEFDGTLQGTPVTLNLSVSNAVPFTADRAFFPHKFAAIPRLTG
                     RISLGEIALKTAVGLMSSETLLKDVESAVDFNVMLKSPHAGRHEATGRLVTAAGKAYV
                     VDGSLKLPSAPVPFDADLAADGTWHIRSQWSGVDAGDLLPSILSGPLSGSLDASGQLK
                     DASKSVAEVRLEARDGEFLGADLEQASKIMREVQPEALPTSAFLRASRTAFTALSCEL
                     SLNGGAWTVRNGSAEGSNWKAAFTGAYGHIDGRVDFTTDNSSAFFTMPARIQADPVLW
                     TPDWTAAHASAREHVGE"
BASE COUNT         1561 a         3025 c         2223 g         1375 t
ORIGIN      
        1 cgtggcgacc gacgtcgccg cccgcggcat cgaccttgaa aaccttacgc acgtcatcaa
       61 cttcggcatt cccgcctcct acgagacgta cgtgcaccgc atcggccgca cgggccgcgc
      121 cggccgcacc ggcaccgcca tcaccattct cgagccgcgc gaacatcgtc acctgcgcgc
      181 catcgagggc atcacgaagg cccgcatcga agtgcgctcc gtgccgtccg ccacggacgt
      241 gcgcgcccgc cgtctcgaaa tgatgcgcgg ctccctcgcc gaaatcctcg aggcgggcga
      301 cctcgaccgc ttccgcgtcg tcgtcgagtc cctctgcgaa gagtacgacc cgttcgacgt
      361 ggctgccgcc gccatgaagc tcgcccagga aaacgaaggc gcgaacgacg acgatacgga
      421 aatccccgcc tactcgccct tcgagcgtcc ccgccgcgaa cgcggcgaca agcccgtgcg
      481 cggcgaacgc gctccgcgcc gtgaacgccg cgaggctccc gaagaaggca tgacgcgcct
      541 cttcgtcggc gccggccgcg acctcggcat tcgtccgtcc gacatcgtcg gggccatcgc
      601 caacgaagcc ggcgttccgt ccaagtgcat cggcgccatc gacatcaccg accgcttcac
      661 gatcgttgaa gtcgactccg accgcgtcga gcagatcatc gacgccatgg aaggcgtgcg
      721 cttcaagggc cgtgaaacca cggtcaagct cgaccgcccg cgccgcgagc cgcgcgaaga
      781 ccgcggtgac ttccgcatcg aaggtcgtgg tgaaggccga ggcgagtacc gccgtgaacg
      841 cggcgaagac cgcggctggc agcagcgcga cgactcccgc gaataccgcg gcgccaagac
      901 caagcgcgac ttccgtcgtg aaggcggcta cgacaagcgc ggtccccgcc gctaaaatga
      961 cgcaggtccg gacagtctcc ttcgagacgc tccggaccca gttttccacg gccggctctg
     1021 ccggcctttt cacgcattga acgtcatgac cgaacaagga attcgattga tcgtcggcct
     1081 cggcaatccg ggcagcgaat acgcccgcac ccgccacaac gccggcttct ggttcgtcga
     1141 cgagctcgcg cgcaagacgg gcgccttctt ccgcgacgag cggaagttcc agggcgaggt
     1201 ctgcaggacg cggatcggcg acagcgacgt ctggcttctc aagccctcga cctacatgaa
     1261 ccgctcgggc gacgccgtgg tggcgcttgc gctctactac aagatcacgc ccgatcagat
     1321 cctcgtcgtt cacgacgagc ttgacctgcc tcccggctgc atgaagatca agcagggcgg
     1381 cggcaatgcc ggtcacaacg gcctcaagga catctccgcc aagctctcca cgccgaactt
     1441 ctggcgcctg aggctcggca cggggcatcc gcgcacgctc ggcatggccc agcaggtggc
     1501 cgacttcgtg ctctcggcac cgtcctccga acacgaggcc gccatcgaag actgcatcgg
     1561 catcgccctc aaggaggccg gtcctcttgc agaaggcgac ttcacgggtg tctcccgccg
     1621 tcttgccaag tacggcaatc cgcccaagcc gcccaagaag gccgccgaat gaagctgctc
     1681 gtgagccgct gcctgctcgg cgagccctgc cgatacgacg gcagggtgaa gacggacctc
     1741 agggcccccc tgcgcgccgc aggcatcgga gacgactgtt ggatcagcgt ctgccccgag
     1801 agcgacggcg gacttcccac gccgcgcacg ccctccgaaa tccggggcac ggcccgcggc
     1861 gtgcttgaag gacgcgacca catcatggcc gcggacggca gggacaacac ggcctgcttc
     1921 atgaagggcg cccgcattgc ggccgacctc tgccgcgaga acggcgtcag gcttgcgctc
     1981 ctcaaggcca agagcccctc ctgcggcttc ggccaggtct acgacggcac ctttacgggc
     2041 accctcgtcg cgggccgcgg cctcaccgcc gaactacttt cggatgcagg cgtacgcgtt
     2101 ctcaccgaga acgacctcga tcttcttgcc tctctcaaga cgcaagaaga tcccgcttgc
     2161 taaaatagaa tgattttccg gacgattttg tctgcaactt ttttttgatt tcaacagaga
     2221 ttcaccatgg gtttgaagtg tggcatcgta ggccttccca acgtcggcaa gtcgacgatc
     2281 ttcaatgcgc ttaccaaagc cggcattgct gccgaaaact atcctttctg caccattgaa
     2341 ccgaacgtcg gcgtcgttga agttcccgat ccccgcctga aggcgctctc cgaaatcgtt
     2401 catcccgaac gcgtcgtccc ggccgccgtc gaattcgtcg acatcgccgg cctcgtcgcc
     2461 ggcgccagca agggcgaagg cctcggcaac cagttcctcg ccaacatccg cgagtgcgac
     2521 gccatcgccc acatcgtccg ttgcttcacc gacgacaaca tcgttcacgt ctccggcaag
     2581 gtcgatccgc ttgacgacat caacgtcatc aacaccgaac tcgccctcgc cgaccttcag
     2641 accgtcgaaa aggccctcaa ccgctacagc aagcaggccc gctccggcgg cgacaaggaa
     2701 gccctcaagc tcgtgctcgt gctcgagaag ctcctgcccg ccctcaacga aggccgtgcc
     2761 gcccgttccg tgaagctcac gcccgaagag atcgccgtcg tgcgcccgct cttcctcctc
     2821 acgatcaagc cgacgatgta cgtcgccaac gtcgaggacc acggctttga aaacaatccg
     2881 ctgctcgacg ccgtgcgtga attcgccgcc tccgagcact cccccgtcgt ggccgtctgc
     2941 gccaagaccg aggccgacat cgccgacctc gacgacgagg acaaggaaat gttcctcgcc
     3001 gacatgggca tgaccgaacc tggcctcgac cgcgtcatcc gcgcgggcta cgacctgctc
     3061 ggcctgcaaa cctacttcac ggccggcgtc aaggaagtcc gcgcctggac gatccacaag
     3121 ggcgacaccg ccccgcaggc cgccggcgtc atccacaccg acttcgaacg cggcttcatc
     3181 cgcgcccaga cgatcgccta cgaagacttc attgccttca agggtgaaaa gggcgctcag
     3241 gaagccggca agatgcgcgc cgaaggcaag gactacatcg ttcaggacgg cgacgtcatg
     3301 aacttcctct tcaacgtctg atcgtcgaag acgcatcatg aacttcaacg ggaggctgcc
     3361 ttcggggcag cctcccgttt tctttccgta tgccgttata ctgaacggcg tcccaacaac
     3421 tccacaacca cgctgtcatg gccacgacct ccacaaaaaa gcgtcaggaa atcggagaac
     3481 gcctccgcca cgaacgcgaa cgacttggct atacgaagca gcagatcgca cagctgctcg
     3541 gcgttccaat ccccgcctac gaatgcttcg aaaacggcga cgacgatcct ggcgtctacc
     3601 gcctgccccg tcttgccgcc tgcggcttcg acgtcatgtt catcatcacg gccgaacgtc
     3661 acgtgcccgt ggctgaggaa aacgaacttc tctcgcgctt tcgcgacctc tcgcagcgcg
     3721 gccgcgcgtc catcttctcc accatggacg cactcgaaag gctggcgccg aacctcaagc
     3781 aggaattccg ccgccgcttc aagaactgaa cgactcccgc ccctccaggg cgggattttt
     3841 cgattcatcg caatagtttt ttacttattc tgttgacacg ggtataaaag ttagctaaga
     3901 taaccccatc aacaaatgag actccttctc acacgtctca taccggagtt aacaaaatgt
     3961 cccttctgaa cactgaaatc cttcccttca aggcccaggc ctacgccaac ggcgaattcg
     4021 tcgaactgac cgaagcctcc ctcaagggtc attggtccgt cgtcttcttc tacccggccg
     4081 acttcacgtt cgtctgcccg acggaactcg aagacctcgc cgaaaactac gccgaattca
     4141 agtccctcgg cgttgaggtc tacgccgtct ccacggacac gcacttcacg cacaaggcct
     4201 ggcatgaaag ctccgccgcc gtgggcaagg ttcagttccc gatgatcggc gacccgaccg
     4261 ccacgctcgc ccgcaacttc caggtgctca tcgaggccga cggccttgcc gagcgcggca
     4321 cgttcgtgat caatcccgaa ggcaagatcg tgatcatgga acatcacgac ggcggcatcg
     4381 gccgcgacgc ctccgaactc ctccgcaagg tgaaggccgc ccagtacgtc gccgccaacc
     4441 ccggccaggt ctgcccggcc aagtggcagc cgggtgccgc cacgctgacg ccgtcccttg
     4501 acctcgtcgg caagatctaa tccagcgatc ggctgaaaca gacaggccgc agacccatac
     4561 ggtcggcggc ctgtttcctt atcctcaaag cgaatgccct catccatcat gcttgacgcc
     4621 aacatcaaag cccagctgca gagctacttc accaaactcc gccgtcccat cgtgctcaag
     4681 gccgcccttg acggctcgca ggacgccacc aacatcgagg cgctcctcaa ggaagtcgcc
     4741 gagctttcgg acaagatctc ctacgaggtc gagtccgacg cctccgttcg ccgtcccagc
     4801 ttcacagtcg cccctcaggg cggtcacgaa ggcattcgct tcgcctgcct cccgatgggc
     4861 catgaattca cgagcttcgt gctcgccctt ctgcagacgg gcggccatcc cgccaagatc
     4921 tccgacgagg agtcccgcca gatcgcagcc ctcaagggcc cgctcaactt cgaggtcttc
     4981 atctcgctct cctgccacaa ctgccctgaa gtcgtgcagg cgcttgacct catctcgatc
     5041 ctcaacccga acgccaccgt caccgtgatc gacggagcgc tcttccgcga ggaggccgca
     5101 ggccgcaacg tgatggccgt tccgacggtc ttcctcaacg gccagtcctt cctctccggc
     5161 cgccgcgaac tttccgaaat cctcgccaag ctcgatacgt ccgtcgccga agcggctcag
     5221 gcctcgatca gcgccaaagc cccttttgac gtactcgttg tcggcagcgg cccggcgggc
     5281 agcacggccg ccatctacgc cgcccgcaag ggcctgcgta cgggcatcgt tgccgaccgc
     5341 ctcggcggcc aggtgaacga aaccgccgac atcgagaact tcacgtccat cctcaagacg
     5401 gacggcaccg cgctcgccgg caacttcacg gcgcatttga agtcctacga catcgacatc
     5461 atcaccccgc actccgtcgc aaagatcgaa cgcgagggcg acatgtggag gctcgtgctc
     5521 gaaaacggct ccgaactccg ctccaagacc atcatcgccg cctccggcgc ccgatggaag
     5581 cagcttgaag tgcccggcga agaggaattc aagggccgcg gcgtggccta ctgcccgcac
     5641 tgcgacggcc cgctcttcaa gggcaagcgc gtcgccgtgg tcggcggcgg caactccggc
     5701 gtcgaggccg ccatcgacct cgccggcatt gcctcctctg tcgtgcttct gcagcgcggc
     5761 tccgagctca ccgccgacaa ggtgcttcag gaaaagctcc tctcgctccc gaacgtcacc
     5821 gtcaagtacc gtgccagcac ctccaagctc acgggctcga acaagcttga aaagctcttc
     5881 tacctcaaca gggaaacggg cgagaccgaa tccatcgacg tcgacggctg cttcgtgcag
     5941 atcggcctca agcccaacac cgaatggctt gacggcgctg tcgagctcaa cgcgtacggc
     6001 gaagtcgccg tctccgaccg ctgcacgacg tcggcccccg gtctctttgc cgccggcgac
     6061 tgcacctcgg ttccctacaa gcagatcgtc atcgccctcg gcgaaggcgc caaggcctct
     6121 ctgagcgcct tcgactacct gatccgtcag ggcgcctgac gacacgatca ctttctccct
     6181 gaaaggggac gggcctgcgc ccgtccccat ttcttttgtg gcacacttgt tcgcgcaaag
     6241 actcaaagag aggattccgc catgcgctat gtgcgctcat tcctgcttgc cctcaccacg
     6301 ctcgccttct tcctgctggt gctcgtcgcc ggcctttacg tcttcatcac gcccgaacgc
     6361 gtctccgccc gtctggccaa cacgctctcg catcatctcg ggctgaagat cgaaatggcc
     6421 aaactgcaca tcgacacccg cctgccgagc ctgaagttca ccgtcaagga cgccgttctc
     6481 accagcgccg acggcgccgt ccgcggcagc gtgccctccg ccacgatcag gcttcatccg
     6541 cttgcactct tcacgcgcag cccccgcatc tccgagatca cgaccgccga cggctcgctc
     6601 accttcggca acacgtctcc cgaagacgtt cgcgcctgga tcgaaggcac ggtcaagccg
     6661 cttgccttca acgtcgacca cctcgtcgtt cacaggggcg ccgtctactt ctccgatccc
     6721 ctgacgggac ccgaaccctg ggcatacgtc aacaacgcct ccgtctcgat cagcaacctg
     6781 agcgaagcgg gtgccgccta cagcctcaac ggcgtcatca agcttcccga aatgctcggt
     6841 agcgccgaac tccgcggcat caccgactgg tcccgaggcc ttcttgaagc cagaaccgaa
     6901 cagctcgacg cctccttcaa gggtgaagtc cgcggccgcc gcaccgaatt cgcagggcac
     6961 gctgacagga tcgcactgac gcgcgaaaac gcctcgctct ccaatgtcgc cgcctccctg
     7021 aagcgcggtg aagagacgct gctcgagctt tcctcccccg ccgtcacgct cgacaggcac
     7081 acacttgaca cccccgtcct gacgacggcc ctcaccgtca tgtcccccga aggctccctg
     7141 accctttccg cctcctcggc cctcaaagcc gacttcggtg cgcgtcgcct cgaggctccg
     7201 tccatcacgg tcgccgcaag cgaaagggcc gcaggcgcaa cgaccgcctc gaatgccggc
     7261 tcccttacgg gcagccttct ctggaacgcc tcttcgggcg agggacgcgc cgaattcgac
     7321 ggcactcttc agggcacgcc cgttacgctg aacctgtccg tctcgaacgc cgtgcccttc
     7381 acggccgacc gtgcgttctt cccgcacaag tttgcggcaa tcccccgtct cacgggacgc
     7441 atatcccttg gagaaatcgc cctcaagaca gccgtcggcc tgatgtcctc cgaaacgctc
     7501 ctcaaggacg tcgagtcggc cgtggacttc aacgtcatgc tcaagtctcc ccatgcaggc
     7561 aggcatgaag cgaccggacg tctcgtcacc gccgcaggca aggcctacgt ggtcgacggc
     7621 agcctgaagc ttccctccgc gcccgttccc ttcgacgccg accttgccgc agacggcacc
     7681 tggcacatcc gcagccagtg gagcggcgtt gacgcaggag accttctccc ctccatcctg
     7741 agcggtcctc tctcgggatc gctcgacgcc tccgggcagc tcaaggacgc ctcgaagagc
     7801 gtcgccgaag tcaggcttga ggcacgcgac ggcgagttcc tcggcgccga cctcgagcag
     7861 gcctcgaaaa tcatgcgtga agtccagccc gaggcccttc ccacaagcgc cttcctgcgc
     7921 gcaagccgca cggccttcac cgcgctatcc tgcgaactct ccctcaacgg cggcgcttgg
     7981 accgtcagga acggttcggc cgaaggctcg aactggaagg ccgccttcac gggcgcctac
     8041 ggacacatcg acggccgcgt cgacttcacc accgacaata gctccgcctt cttcacgatg
     8101 cccgcccgca tccaggccga ccccgtcctc tggacgccag actggacggc cgcccacgcc
     8161 tccgcccgcg aacacgtcgg cgag
//
LOCUS       DBMDTI010000073         8116 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2390_length_8116_cov_3.408609, whole genome shotgun sequence.
ACCESSION   DBMDTI010000073 DBMDTI010000000
VERSION     DBMDTI010000073.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8116)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8116
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_8116_cov_3.408609"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            3..1232
                     /locus_tag="ACZPB6_04975"
     CDS             3..1232
                     /locus_tag="ACZPB6_04975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1183858.1"
                     /translation="MRASGLFNASDPARQEPMGITDSPSWHYEQTMASGHGRNDPAVV
                     RRFVCEALPTLKWLESLGVRFMPETVATWGAEWPRGHKPTQPRGQAYIRTLSSECIRR
                     GVKIRTRCAMTRLLMKNGRAASIEVEDLSSGRGKDKMVFHARKAVILTAGGFAANPEL
                     VESYRADLAGIPTDNNPGSTGEVMLEAIRAGAASNALECIQCVPGAPEGRGFQVRLDL
                     DAGRVLMVDGGGRRFIDEDGSRDGLAKAILARPRGSVWMVTDDGAVKAMDLISRKDVY
                     RGLQTGDALRADSWDALALLMGVDADALKDSVRVFNVEAGMGGGKCARVRCLQLSEPP
                     YWASRVVLNIHTTMGGLVITPDAQVVGLDGKPVTGLFAAGEITGSVHGENRMGGNGIA
                     DALTFGRIAGRTAVLKR"
     gene            1364..2848
                     /locus_tag="ACZPB6_04980"
     CDS             1364..2848
                     /locus_tag="ACZPB6_04980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1183859.1"
                     /translation="MTTSRREFFKTAAMGAVGAGAAFGTVAAEAKAAAPSKYDIIILG
                     AGCGGLTCAVRAAENGLKPLLLEKMAMPAGNTIYAAGFLLGVNTSFQKAKNVQGDSAE
                     QFLKDWMKVTQGKADEKLARVVCENADATLEWLHGYCGINFAAGSKLVWPMLSRAHLV
                     VGEHKPGGGELALTLMKKAKELGVEIRFNTKAVALLSDEKTGAVNGVRVKTKEALSKI
                     HSKFGVVLATGGFSANQAMVTQYIGSGGAKMPIRGSRIIAGENINLALPFMPKIVNVD
                     QYHCGPIYGPTGANPLNIVNNGICVNDKGERFTDEGQTYVQMSRDVAAMTKANWAYMI
                     VDQAGHDVPILANDWESYKRTKAPVYVGQTLDEAAEKAGLPVDAVKATVKAYNEAIAA
                     GKGKTLTPVNTLPKARTVEKAPFYIVPFQGGMTATFGGPLINVKGQVLDTENHVIDGL
                     FAIGNAAGGLFYDNYIGGAQLTSAAVYGIVVADFVKAAKTPAGK"
     gene            3099..3560
                     /locus_tag="ACZPB6_04985"
     CDS             3099..3560
                     /locus_tag="ACZPB6_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183860.1"
                     /translation="MFKKTIAMTGAAALLALGLTGCGATLGGGAQSEEAAASADNQLC
                     VIPRTPTPQLLEAVSAGLKTAGYEVKVLPEDAKTDACDHCVLYGLMLNEKRDGVKAFV
                     FQSFKNGQPELAANAPVENNQVNLQQVAQYAVEFAAALKKNGEAGGTPAAR"
     gene            3729..4430
                     /gene="uppS"
                     /locus_tag="ACZPB6_04990"
     CDS             3729..4430
                     /gene="uppS"
                     /locus_tag="ACZPB6_04990"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR1183861.1"
                     /translation="MSASHIPHHVAIIMDGNGRWAKSRLRPRTEGHRQGVEALNRVVE
                     AAARAGIERLTVFAFSSENWRRPAAEVSMLMKLFAQALRRWCEPLAEAGVRLRVIGDR
                     TAFSDTVREAIAENEAATAGGTKMELVIAANYGGRWDCCQAASECAAAGLEMTPENIG
                     RFMSVPDVDLLIRTGGECRISNFLLWQSAYAEIYFTDALWPDFGEDDLKEALRWYEGR
                     ERRFGRTSEQLRNGG"
     gene            4786..5634
                     /locus_tag="ACZPB6_04995"
     CDS             4786..5634
                     /locus_tag="ACZPB6_04995"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR1183862.1"
                     /translation="MLLTRVITAAVLLAVLTLSIYIGPFAFASVMAIAFGATLYEWLR
                     IGGLGPRPALLVAAVEMVVQLSLYWAGALDRMNWFLMLMNGCVMLAWFVIFFAELFHR
                     ENGFKVSQRQCLWSAVTFVPAAYLSLLWLFEQGDWVLVLSVLLIVWGADISAYFCGRA
                     WGKHRMAPAISPKKTFEGAIGAYVIVIVFFALTYVFCVQQNVFTNYLFDNAGFLWGVG
                     VLAMLVALSIAGDLWESMLKRLVGIKDSSNLLPGHGGFFDRMDASLPVLPATAFIMLW
                     IQLIVH"
     gene            5712..6344
                     /locus_tag="ACZPB6_05000"
     CDS             5712..6344
                     /locus_tag="ACZPB6_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HIR1183863.1"
                     /translation="MTDAYKVPDLAPGAFHRAEETVRRSRFIVTMARVSSPEEAKAFI
                     DRIREEHAGATHNCWAYNAGAPGDTAQVGASDDGEPKGTAGRPMLTALLRSDVGEVAA
                     VVTRYFGGILLGTGGLVRAYQGSVKLGLEGLPVTLREDMRRFVVSIEPHQVSDFQHFL
                     AEARGRVQSSDFRFDATFEVLVPSDEADAFVERLSEATAGEALIDPIDEE"
     gene            6526..7668
                     /locus_tag="ACZPB6_05005"
     CDS             6526..7668
                     /locus_tag="ACZPB6_05005"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1183864.1"
                     /translation="MRFAIFHAVCALWVVWRFWLPMNAPQVVKVAGFVVTLLIAAFPA
                     VTATFFGGLVSPELPAWVLAAGNGCVFALTVLAGLTLLREVVIFLSVLAGRSGERMHR
                     FVQKDRRVAIGMTAAAVGISAFGLRQGLRVPCVKEMTVPIEDLPAGLEGMRIVQLSDL
                     HASALLRAPHMKDLVARVNSLSPDLVVITGDLVDGEVDVRSEDVAPLAELRARHGVIA
                     VEGNHEHYLDYEGWMKKIPSLGIRLLRNEWRAVEHNGATLVVGGVTDPWARRFGRELP
                     DPIKAFAGAPEKGPRILLSHQPKHARKYDEAVRFDLLLSGHTHGGQLFALTELVAILN
                     ATFVRGWYALKHARLYVHSGSGVWNGFPVRVGVPSEIAVFTLSRKA"
     gene            7789..>8116
                     /locus_tag="ACZPB6_05010"
     CDS             7789..>8116
                     /locus_tag="ACZPB6_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR1183865.1"
                     /translation="MTQSITILGATGSIGRSTLDVVSRYPERFSVHAVAGGSRVEPLV
                     DVCLSARPKRAVIADASKVVELEQALCTVGLGDIEVLGGPEAVSRLADDPDTDVVVQA
                     VVGAAGV"
BASE COUNT         1499 a         2386 c         2573 g         1658 t
ORIGIN      
        1 cgttgcgtgc aagcggtctg ttcaatgcgt ctgatcctgc ccgtcaggag ccgatgggta
       61 tcacggactc cccttcctgg cactatgaac agacgatggc gtcaggacac ggccgaaacg
      121 acccggctgt tgtgaggcgc ttcgtgtgcg aagcgttgcc aaccttgaag tggcttgagt
      181 ctcttggcgt tcgattcatg cccgaaactg tcgccacctg gggcgcagaa tggccgcgcg
      241 gccacaagcc cacgcaaccg agggggcagg cctacatccg gacgctttcc tcggagtgca
      301 taaggcgtgg cgtaaagatc cgcacaagat gtgccatgac gaggcttcta atgaagaacg
      361 gccgagcagc aagcatcgaa gtcgaggatc tttcctccgg aagagggaag gacaagatgg
      421 tctttcatgc cagaaaggcc gtcattctga cggcgggagg ctttgcggcg aatcctgaac
      481 tggtggaaag ctacagggct gaccttgctg gcattcctac ggacaacaat cctgggagta
      541 cgggcgaggt catgcttgag gcgataaggg ctggtgccgc ttcgaacgcg ctcgaatgca
      601 ttcagtgcgt gccgggcgcc cctgaaggac ggggcttcca ggtgagactt gatctggacg
      661 cgggccgtgt tctgatggtg gatggcggag gacgccgctt tattgacgag gacggctcgc
      721 gtgacggact cgcgaaggcg atactcgcca gacccagagg gtccgtctgg atggtgacag
      781 acgatggcgc ggtcaaagcc atggatctta tcagcagaaa ggacgtctac agagggttgc
      841 agacgggcga cgcgctccgt gccgattcct gggacgcatt ggctctgctg atgggtgtcg
      901 atgcagatgc gctcaaggac agtgtccgtg ttttcaacgt cgaagcaggc atgggcggcg
      961 gcaagtgcgc gcgtgtacgc tgcttgcagc tgtcggagcc gccctattgg gcgtcaagag
     1021 tcgtcctcaa tattcacacg acgatgggag gactcgtcat tacgcctgac gcacaggttg
     1081 ttggactcga cggcaagccg gtgaccggac tctttgcagc tggggagatc acggggagcg
     1141 tgcacggtga aaaccgcatg ggcggcaatg gcatcgccga tgcgctgact ttcggtcgga
     1201 ttgcgggaag gacggcggtt ctcaagcgtt gagatcactt tttgtcagtc ttgtcaagat
     1261 cccgatgtgc tttagattcc atcgggagga gcctatagtc gatttcaagc gatgcgggag
     1321 cacaaccgca tcgggaccat aaagactaat ggagaacagg caaatgacta cctctcgtcg
     1381 tgaattcttc aagaccgccg ccatgggagc ggttggtgca ggtgctgcat tcggcacggt
     1441 tgccgctgaa gccaaggctg cggccccctc caagtacgac atcatcattt tgggcgctgg
     1501 ttgtggcggc ctcacctgtg ccgtgcgcgc ggctgaaaac ggcctcaagc cgcttcttct
     1561 tgaaaagatg gccatgcctg cgggcaacac gatttatgct gcgggcttcc tccttggcgt
     1621 caatacctcc ttccagaagg ccaagaatgt tcagggcgat tccgccgaac agttcctcaa
     1681 ggactggatg aaggtcacgc agggcaaggc tgatgaaaag ctcgctcgtg ttgtttgcga
     1741 aaacgccgat gccacccttg aatggcttca cggttattgc ggcatcaact ttgcggctgg
     1801 ttccaagctt gtctggccga tgctttcccg cgcacatctc gttgttggcg aacacaagcc
     1861 gggtggcggc gagctggccc tgacgctcat gaagaaggcc aaggagcttg gtgtcgagat
     1921 ccgcttcaat acgaaggccg tggcactgct ttccgatgaa aagacgggcg ccgtgaacgg
     1981 tgtgcgcgtc aagacgaagg aagctctctc caaaattcat tcgaagttcg gcgtcgtgct
     2041 ggccacgggc ggcttctccg ccaatcaggc catggtcacg cagtacatcg gttcgggtgg
     2101 tgccaagatg ccgatccgcg gttcgcgcat catcgcaggt gagaacatca atcttgcgct
     2161 cccgttcatg ccgaagatcg tcaacgttga ccaataccac tgcggtccga tctacggacc
     2221 gaccggcgcg aacccgctca acattgtcaa taacggcatc tgcgtcaatg acaagggcga
     2281 acgcttcacg gacgaaggcc agacctacgt gcagatgtct cgagacgttg cggccatgac
     2341 caaggccaac tgggcgtaca tgatcgtcga ccaggcggga catgacgtgc cgattcttgc
     2401 caacgactgg gagtcctaca aacgcaccaa ggctcccgtg tacgtcggtc agacgcttga
     2461 cgaagcggcc gaaaaggcag gtcttcccgt tgacgccgtc aaggccactg tcaaggctta
     2521 caacgaggcg atcgctgcgg gcaagggcaa gaccctgacg ccggtcaaca ccctgccgaa
     2581 ggcacgcacg gttgaaaagg caccgttcta tatcgttccc ttccaggggg gcatgacggc
     2641 tactttcggc ggtccgctca tcaacgtcaa ggggcaggtg ctcgatacgg aaaaccacgt
     2701 tatcgatggt ttgttcgcca ttggcaacgc tgcgggcggt ctcttctacg acaactacat
     2761 tggcggcgcc cagcttacga gtgcggcggt gtacggcatc gtggtcgccg attttgtcaa
     2821 ggcggccaag actcccgccg gcaaatgaac ggcttcagat gaactgagct accacagcgg
     2881 ctgttgctct tgagatgagc gcaagcatgc cgctcttata aaggggactc ctttgcaaaa
     2941 gaggagtccc cttgttgcct gatgctccca gcttcgcgga cggcgatttg caggatttct
     3001 aaatcagaat cattgtcatt ggcctaagtg ttcgcgtatg attgaagaac ggcctccagt
     3061 cggagggttg actgaaccat ttgcggagag acaaaagaat gttcaagaag actatcgcca
     3121 tgactggcgc tgcggcgctt ttggccctgg gcctgacggg ctgcggtgca actctgggcg
     3181 gaggcgccca gtccgaggag gctgccgcgt ccgcagacaa ccagctctgc gtaattccgc
     3241 gcacgccgac cccgcagctg cttgaggccg tgtccgcggg gttgaagact gcgggctatg
     3301 aagtcaaggt cctgcccgaa gacgccaaga ccgacgcctg cgatcattgt gtgctctacg
     3361 gcctcatgct caacgagaag cgcgacggcg tgaaggcctt cgtcttccag agcttcaaga
     3421 acggtcagcc tgagcttgcc gccaacgctc ccgttgaaaa caaccaggtg aacctgcagc
     3481 aggtcgcgca gtatgcggtc gagtttgcag cggcgctcaa gaagaatggt gaggcgggtg
     3541 gcactcctgc ggctcggtaa ttcctctctc cggaagaaca gcggcatccg gcaggcttgc
     3601 gggtctgccg gatgtttgtt ttggctagcg gacaaatacg ttacgggatg cgttccgggg
     3661 ctgttctcag cgcctcggcg tgtaaaatgc ctgccttgta caaacaccgc ttttcctgct
     3721 cctttctcat gtccgcctcc cacattcccc atcacgtcgc gatcatcatg gacggcaacg
     3781 gccgctgggc caagtcgcgt ctgcgtccga ggacagaggg gcacaggcag ggcgtcgagg
     3841 cgctcaaccg cgtggtggag gcggccgcca gggcaggcat cgaacgcctg acggtattcg
     3901 ccttttcgag cgaaaactgg cgcaggcccg ccgcagaggt ctcgatgctc atgaagcttt
     3961 tcgcacaggc gcttcgccgc tggtgcgagc cgctcgccga ggcgggcgtc aggcttcgag
     4021 tcatcgggga caggacggcc ttttccgata cggttcgcga ggccatcgct gaaaatgaag
     4081 ctgccactgc gggtggcacg aagatggagc tcgtcattgc ggcgaactac ggcggacgct
     4141 gggactgctg tcaggcggcg tccgaatgcg ccgcggcagg gcttgagatg acgcccgaga
     4201 atatcggaag gttcatgagc gtgcctgatg tcgatctttt gattcgtacg ggcggcgaat
     4261 gcaggatttc caactttctt ttgtggcaga gtgcttacgc ggaaatctac ttcaccgatg
     4321 cgctctggcc cgacttcggc gaggacgact tgaaggaggc tcttcgctgg tatgaggggc
     4381 gcgagcgccg cttcggtcgc acgagcgaac agctcaggaa cggcggctga agtcaggggg
     4441 gctttccgac tttccgacaa catggatgta gagacggcct gcctttcagc gggactttct
     4501 tgtgcgctcc aaccgaagag gcggaagaac gggagtgctc gtcttcgcct gaatgcagcc
     4561 gcgttttcgg ctttctgggt ttcggatgca ggacatgagg cctgcagtga ggagcgggcg
     4621 caggcctgaa caagacggtg ttttcaacgc gcccctttga ggaattcggc cacgcgaggt
     4681 cctgcattgg ctaaaatggc aggatgctgg cctttatatg aaaggcgaca cccccataga
     4741 catacaacgc gccggtcacg agaaccggcc ggaggttcct tcatcatgtt gcttacgcgc
     4801 gtgatcaccg cagccgtgct gctggccgtt ctgacgctct cgatctacat tggtccgttt
     4861 gcctttgcaa gcgtcatggc cattgccttc ggcgccacgc tctatgagtg gctgcgcatc
     4921 ggcggcctcg gtccccgtcc cgctcttctc gtcgccgccg tcgaaatggt agtccagctt
     4981 tcgctctact gggcgggtgc gcttgaccgc atgaactggt tcctgatgct catgaacggc
     5041 tgcgtgatgc tggcctggtt cgtgatcttc tttgcggaac tcttccatcg tgaaaacggc
     5101 ttcaaggtct cgcagcgcca gtgcctctgg tccgccgtga ccttcgtgcc cgccgcctac
     5161 ctgtcgcttc tctggctctt tgaacagggg gactgggtgc tcgtcctctc cgtgctcctt
     5221 atcgtctggg gcgccgacat ctccgcctac ttctgcggtc gcgcctgggg caagcaccgc
     5281 atggcgcccg ccatttcgcc caagaagacc ttcgagggcg cgatcggcgc ctacgtgatc
     5341 gtgatcgtct tcttcgcgct cacgtacgtc ttctgcgtgc agcagaacgt cttcacgaac
     5401 taccttttcg acaatgcggg cttcctgtgg ggcgtcggcg tgctcgcgat gctcgttgcg
     5461 ctctcgattg ccggcgacct ctgggagtcg atgctcaagc gccttgtcgg aatcaaggac
     5521 tcttctaacc tgctgcccgg ccacggcggc ttcttcgacc gcatggacgc ctcgctcccc
     5581 gttctgcctg ccacggcctt catcatgctg tggatccaac tgatcgttca ctaagcctga
     5641 actgaggatc atgcagaggg cgcggtaccg acaactcggc ccgcgcccgt tcttattttg
     5701 gaccctgatt catgaccgac gcctacaaag tccccgacct tgcgcccggc gcctttcacc
     5761 gggctgaaga gaccgtgcgc cgctcccgct tcattgtgac gatggcgagg gtttcgagtc
     5821 ccgaggaggc gaaagccttc atcgaccgca tccgagagga gcacgcgggc gccactcaca
     5881 actgctgggc ctacaacgca ggcgcacccg gcgacacggc tcaggtgggc gcgagcgacg
     5941 acggagagcc aaagggcacg gcgggacgtc ccatgctcac cgcactcctg cgcagtgacg
     6001 tgggtgaggt ggcggccgtc gtgacgcgct acttcggcgg catcctgctc ggtactggcg
     6061 gtcttgtcag ggcgtatcag ggttcggtca agctcggact tgagggcctt cccgtcacgc
     6121 ttcgcgagga catgcgccgc ttcgtggtct cgatcgagcc ccatcaggtt tcggacttcc
     6181 agcactttct cgccgaggcg aggggacgcg tgcagtcgtc ggacttccgc tttgacgcaa
     6241 ccttcgaggt gctcgtcccg tcggacgagg cggacgcctt cgtcgagcgc ctgtcggagg
     6301 ccacggcggg cgaagcgctc atcgatccga ttgacgagga gtaggtcctc agggagattc
     6361 aagcccgggg ggatcccatg aatgtgactg tatctgtcag gctgcaagtc ttcgcaagag
     6421 aaggatcgcg tgaaaacatc gcggtctaga atggggtcat gggcagttcg ccctgaagga
     6481 tttgccggcc gcaaggccta aacaggacga gaggattggt tcgccgtgcg ttttgcaatt
     6541 tttcatgccg tctgtgcgct ctgggtggtc tggcgctttt ggcttccgat gaatgcaccg
     6601 caagtggtga aggtcgcggg ctttgttgtg acgcttctca tcgccgcctt tcccgccgtc
     6661 accgccacgt tcttcggcgg gctcgttagc cccgagctgc ccgcctgggt gctggccgcc
     6721 ggcaacggct gcgtcttcgc gctcaccgtg cttgcaggcc tcacgctgct tcgcgaggtc
     6781 gtcatctttc tgtccgttct ggccggccgt tcgggagagc gcatgcatcg cttcgtgcag
     6841 aaggacaggc gcgtggcgat cggcatgacg gccgcggccg tcggcatttc ggccttcggt
     6901 cttcggcagg gactcagggt tccctgcgtc aaggagatga cggttccgat cgaggacctg
     6961 cctgcgggac ttgagggcat gaggatcgtg cagctctccg acctgcatgc gtcggcgctt
     7021 ctgcgcgcgc cccacatgaa ggatctcgtg gcaagagtca acagcctctc gcccgatctt
     7081 gtcgtcatca cgggcgacct cgtcgacggc gaggtggacg tcaggtccga ggacgttgct
     7141 ccgctcgcgg aacttcgtgc acggcacggc gtcatcgcgg tcgagggcaa tcacgagcac
     7201 taccttgact acgaaggttg gatgaagaag attccgtcgc tcggcataag gcttctgaga
     7261 aacgagtgga gggccgtcga gcacaacggg gccacccttg tcgtgggcgg cgtgacggat
     7321 ccttgggcaa ggcgcttcgg acgcgagctg cccgatccca tcaaggcctt tgcgggcgca
     7381 cccgaaaaag gaccgagaat tcttctttcc catcagccca agcacgcccg aaagtacgac
     7441 gaggctgtgc gttttgacct gctgctttcg gggcacaccc atggcgggca gctctttgcg
     7501 ctcaccgagc tggtggcgat cctcaacgcg accttcgtgc ggggctggta tgcgctcaaa
     7561 catgcgaggc tctacgtgca ttcgggctcg ggcgtctgga acggcttccc cgtgcgcgtc
     7621 ggggtgccga gcgagatcgc cgtcttcacg ctatcccgaa aggcctgagg cttaaggccc
     7681 gggcgttcgg ccgcgggtaa aatactgcgc agaacaatgg aagcctgacg gcaaggaccg
     7741 caggccgacc gacaggaaca atcgaatcaa caactgacaa ggggcaacat gacacagtcc
     7801 atcacgattc tcggcgcaac gggtagcatc ggccgttcga cgcttgacgt cgtctcgcgc
     7861 tatcccgagc gcttcagcgt tcacgccgtg gcgggcggct cccgcgtgga gccgctggtg
     7921 gacgtctgcc tctctgcacg tccgaagcgt gccgtcattg cggatgcctc gaaggtggtg
     7981 gagctggaac aggccctctg cacggtcggc ctcggcgaca tcgaggtgct cggcggcccc
     8041 gaggccgtct cccgcctcgc ggatgatccc gacacggacg tcgtcgtgca ggccgtggtc
     8101 ggcgccgcgg gtgttg
//
LOCUS       DBMDTI010000074         8094 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2401_length_8094_cov_2.365320, whole genome shotgun sequence.
ACCESSION   DBMDTI010000074 DBMDTI010000000
VERSION     DBMDTI010000074.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8094)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2401_length_8094_cov_2.365320"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(57..362)
                     /locus_tag="ACZPB6_05015"
     CDS             complement(57..362)
                     /locus_tag="ACZPB6_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183866.1"
                     /translation="MMDLSDFTAQVQAAHWGWTIALFLWLVGLSGMGMFLNNWVREKA
                     LVYVCTITGIVGTLLVVSHLTRILNLPMAAISALLEMSFNFGSWMFIGICLLVDYII"
     gene            complement(359..937)
                     /locus_tag="ACZPB6_05020"
     CDS             complement(359..937)
                     /locus_tag="ACZPB6_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1183867.1"
                     /translation="MKKVKKQLAHLFDATQCINCGACTTACAQTNYPDLINEFIPAWN
                     TVCNNIRQVKVEKNRPMQLLVQCQQCSEAPCVTTCPFGANYYDENGLVRNDPKRCIGC
                     NYCVASCPYDARWSNPKTGLPSKCMGEGCLELIKGGSLPACVTACPAGARLFGDTLDP
                     QSEISRKIASSKTQTLLESHGTKPNFYVVVQK"
     gene            complement(944..3520)
                     /locus_tag="ACZPB6_05025"
     CDS             complement(944..3520)
                     /locus_tag="ACZPB6_05025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR1183868.1"
                     /translation="MADTIVDLRMSRRGLIKLGGAAAVATTGAAGAMWPIEAQLKELE
                     HQGWSRHPLACCMCGAFCGLVAMKKIGEPTSEKTVRIFPNPDHPQRGYCGRAAATMWI
                     WNHPLRIRKPLKRVGERGEGKFKEVSWDEALNDIAAKLKDVVAKHGEQSVVSTSHSFS
                     SYSKWITFPLGSPNDIGHSSTCNSASIAGRDWVYGKGFSGAGKIEPDYKHLRYLILIG
                     RSMGSAMGCLHSLNEARERGAQVVSVDPRMPDIAYGDAKWVPIRPGTDASFVLAMMNV
                     MLQEGLADLKFLAKHTNAAYLIKADGRPLTEADMKAGGRKDRYAVHDLKTNRIVFQGV
                     VLDEKGAAVGFDESADTDANLHFAQEVKLADGTTTAVRTALDILAEIAARHTPEASQK
                     ITGIPADELVRLAHNFANLKGVVDDGWYSSKNGNDIELYQLINILNAFNGNVDREGGI
                     VVTAGGGFKRPGVAAGKGPNGEKWDMVDAKRIDKMLSPESAGNLWAALDAMITGKPYP
                     IRALFTVGTTLFHRESDSTRLAKALKTLDLLVVQDLLPHEVCDYADYVLPATYFLERR
                     ETAGVKWALDGSVHMNDAGIRPPEGVEARHDVWILLEILRRAYPERAERVGYKECKTA
                     DEFDAWWNKFDDKGIAKFVKDQEAKNPGSGEKILADFKTKGWTTVNKKKYGVYPWKKP
                     FGTPTGKIEIYGFKSFAKPGYEKIKPISDYFPAPAYTAPKPQSNEFVLVSGKNCTSSS
                     GLNIFAATTRFTGDRTLWMNPYDAERLGIANKEKVMVEGVDREYQAEVVVTVTKKVMA
                     GSVFAFGFSGGVRTKELVNDPRFDFVKEGINSHWFATGYAQPVVGNLANNSCIRVKRI
                     KG"
     gene            3974..5899
                     /locus_tag="ACZPB6_05030"
     CDS             3974..5899
                     /locus_tag="ACZPB6_05030"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1183869.1"
                     /translation="MSGPLSRALAGLLFVLMLVPGVSAASDDGMLVPDSAHAAAESET
                     ASVKRLRLAIVNSYGMSIMDDYYSRTIEAVKEKLSPIEIDVKIYGPDSFLKAAEKESF
                     DMSIASSGLTSLMIERTGGLPLAAVVSDEAPDPNYANGAAIIVRADRPELRTLEDLRG
                     RSVAIMSRTAFAGWQIPASEMVRKGLDPEVFFSEIRVSGYPMTRIVQEVKSGEVDVGF
                     VATCLLERMARAGQIDQKDFRVIGEHADRLVACRHSTELYPNWFFSIKPSVPTSVART
                     LTATLLDVPAAPDGTQWTISNDNRRLFEVFRVLQVPYGNPDDFGRFVRRILPWVVGAG
                     GVLLILLLNTLLLARLASHRARALEKAMDEKLRAELAAQQNAVQIESLTRASAVGLLS
                     SMVAHELKQPLTVIGNYAGSLRRRLSRGEVPHETLLASITEIEDSGTRAAAIIDRVRS
                     YGQVRTRSLAAVDLSDLVKTAVARWRRHSAHRVPCTLDLEPGLTVEADRLEIELAVLN
                     IVKNAAAAVKDQPDAAIEVTLKADGAYARLCIADNGPELSDEEIARLGDIGYTTTAGG
                     LGLGIAIVHSLLEAHGGSLAYRRRTDRPRRPRPRLHREAPALRRPRGQDRRLSRRRIP
                     PLCFQLPRFPCGIRQNA"
     gene            complement(6168..7451)
                     /locus_tag="ACZPB6_05035"
     CDS             complement(6168..7451)
                     /locus_tag="ACZPB6_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1183870.1"
                     /translation="MEDKTKYYEEMVRNRRELHKRPEEGWTEFETTYFVVSFLRNLGL
                     PVTVGKANINESEVLGRDPQLVKDGMVRALKHGVPQSFLDETEGYTGAVAVLDTGRPG
                     PTTAFRADMDCVLVRETDDEAHLPNRLGFASERPGFMHACGHDGHTAVGLEVARWLVD
                     HKNELCGRFKLIYQPAEEGVRGARAMAAAGVVDDVDYFISGHVGGLAKLGEIGVMDGG
                     FLASTKFDIDIEGQPAHAGNAPQLGHNALMAACAASMMLQGIPRNGDGTTRVSVGTLH
                     AGEGRNVIPAHARLQMEVRGETEEVNGYMRDYVYDIFSGVDKAYRVKSTVTMAGESTT
                     LLQSPELFDKVEEVMKTVPNTTMLPRIHAPSGSEDCACFIRRVIQKGGQAAFILWGCN
                     HHGHHRQNFDIQDESSMPNAFNVYTGFARKMNGIE"
     gene            complement(7470..>8094)
                     /locus_tag="ACZPB6_05040"
     CDS             complement(7470..>8094)
                     /locus_tag="ACZPB6_05040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1183871.1"
                     /translation="EAPSMNWLEMVRTPLFYPLFILFTSSAMMGLMLLSNISAIAQEQ
                     VGAGAGLAALGVSVISIANTSGRFLSGTLSDRIGRIPTLVLVLVVALAGLGMLVSAGH
                     GDTGLFFAGIVGIGTCFGAFIGTYPSLVADEYGPKHNSVNFSLMMFGYSVGGLTGPLL
                     IRWANAGGDFSRAYIVCVAAAVVAIVCALIAIRLKHCALPVADKASA"
BASE COUNT         1467 a         2575 c         2535 g         1517 t
ORIGIN      
        1 atgagtacaa tatcctgcta aagcttcatc aatactgtta tctacaactt catatactaa
       61 atgatgtaat ccacgagcag gcagatgccg atgaacatcc agctgccgaa gttgaagctc
      121 atctcaagca gggccgagat ggcggccatc ggcaggttga ggatgcgcgt cagatggctg
      181 acgacgagaa gcgtgcccac gatgcccgtg atcgtgcaga cgtagacgag cgccttctcg
      241 cgcacccagt tgttgaggaa catccccatg cccgagagac ccacgagcca gaggaagagc
      301 gcaatcgtcc agccccagtg ggcggcctgg acctgcgcgg tgaagtcgga gagatccatc
      361 atttctgcac caccacatag aagttcggtt tggtcccgtg gctctcaagg agcgtctggg
      421 tcttggaaga ggcgatcttg cgggagattt cggactgcgg atcgagcgtg tcgccgaaga
      481 ggcgggcgcc cgccgggcag gccgtcacgc aggcaggcag gctgccgccc ttgatgagct
      541 ccagacagcc ctcgcccatg cacttgctcg gcaggcccgt cttcgggttg gaccagcggg
      601 cgtcgtacgg acaggaggcc acgcagtagt tgcaaccgat gcagcgcttc ggatcgttgc
      661 gcacgaggcc gttctcgtca tagtagttgg cgccgaacgg gcaggtcgtg acgcagggcg
      721 cttcggagca ctgctggcac tggacgagga gctgcatcgg acggttcttc tcgaccttga
      781 cctgacggat gttgttgcac accgtgttcc aggccgggat gaactcgttg atgaggtccg
      841 gatagttggt ctgggcgcag gcggtcgtgc acgcgccgca gttgatgcac tgggtcgcat
      901 cgaagaggtg cgccagctgt ttcttgactt tcttcatggt gacttatccc ttgatgcgct
      961 tgacacggat gcagctgttg ttggcaagat tgccgacgac gggctgcgca tagccggttg
     1021 caaaccagtg cgagttgatg ccctccttca cgaagtcgaa gcggggatcg ttgaccagct
     1081 ccttcgtgcg gacgccgccg gagaagccga aggcgaacac gctgccggcc atgaccttct
     1141 tcgtcacggt gacgacgacc tcggcctggt actcgcggtc aacgccttcg accatgacct
     1201 tttccttgtt ggcgatcccg aggcgctcgg cgtcgtacgg gttcatccag agggtgcggt
     1261 cgcccgtgaa gcgggtggtc gcggcgaaga tgttgagacc cgaggaggac gtgcagttct
     1321 tgcccgagac gagcacgaac tcgttgctct ggggcttcgg agccgtgtag gcgggagccg
     1381 ggaagtagtc gctgatcggc ttgatcttct catagcccgg cttcgcgaag gacttgaagc
     1441 cgtagatttc gatcttgccc gtgggcgtgc cgaagggctt cttccaagga tagacgccgt
     1501 acttcttctt gttgaccgtg gtccagccct tggtcttgaa gtcggcgagg atcttttcgc
     1561 cggagcccgg gttcttggct tcctggtcct tgacgaactt ggcgatgccc ttgtcgtcga
     1621 acttgttcca ccaggcgtcg aactcgtcgg ccgtcttgca ttccttgtag cccacgcgtt
     1681 cggcgcgttc cggataggcg cggcggagga tttcgaggag gatccacacg tcgtggcggg
     1741 cctcgacgcc ctcgggcgga cggatgccgg cgtcgttcat gtgcacggaa ccgtcaagcg
     1801 cccacttcac gccggcggtt tcgcgacgtt cgaggaagta ggtggccggc agcacgtagt
     1861 cggcgtagtc gcacacctcg tgcggcagca ggtcctgcac gacgagaagg tcgagcgtct
     1921 tgagggcctt ggcaaggcgc gtggagtcgg actcgcggtg gaagagcgtg gtgcccaccg
     1981 tgaagagcgc gcggatcgga tagggcttgc ccgtgatcat cgcgtcgagc gcggcccaga
     2041 ggttgcccgc ggattccgga gagagcatct tgtcgatgcg cttggcgtca accatgtccc
     2101 acttctcgcc gttggggccc ttgcccgcgg cgacgcccgg acgcttgaag ccgccgccgg
     2161 cggtcacgac gatgccgcct tcgcggtcga cgttgccatt gaaggcgttg aggatgttga
     2221 tgagctgata gagctcgatg tcgttgccgt tcttggagga ataccagccg tcgtcaacga
     2281 cgcccttgag gttggcgaag ttgtgggcga gcctgacgag ttcgtcggcc gggatgcccg
     2341 tgatcttctg ggaggcctcg ggggtgtggc gggcggcgat ttcggcgagg atgtcgagcg
     2401 cggtgcgcac ggcggtcgtc gtgccgtcgg caagcttcac ctcctgggcg aagtgcaggt
     2461 tggcgtccgt gtcggcggat tcgtcgaagc ccacggcggc gcccttttcg tcgagcacga
     2521 cgccctggaa gacgatgcgg ttcgtcttga ggtcatgcac ggcgtagcgg tccttgcggc
     2581 cgccggcctt catgtcggct tcggtgagcg gacggccgtc ggccttgatg aggtaggcgg
     2641 cgttcgtgtg cttggcgagg aacttcaggt cggcgaggcc ttcctggagc atgacgttca
     2701 tcatggcgag cacgaaggag gcgtcggttc ccggacgaat cgggacccac ttggcgtctc
     2761 cgtaggcgat gtcgggcatg cgcgggtcga cgctcaccac ctgggcgccg cgttcacggg
     2821 cctcgttgag cgagtgcagg cagcccatgg cggagcccat ggagcggccg atgaggatca
     2881 ggtagcgcag gtgcttgtag tcgggctcga tcttgccggc gcccgagaag cccttgccgt
     2941 acacccagtc gcgaccggcg atcgaggcgg agttgcaggt gctcgagtgg ccgatgtcgt
     3001 tcggggagcc gagcgggaac gtgatccact tggagtagga ggagaaggag tgcgacgtgg
     3061 agacgacgga ctgctcgccg tgcttggcga cgacgtcctt gagcttcgcg gcgatgtcgt
     3121 tgagggcttc gtcccaggag acttccttga acttgccttc accgcgttcg cccacgcgct
     3181 tgagcggctt cctgatgcgc agcgggtggt tccagatcca catcgtggcg gcggcgcggc
     3241 cgcagtagcc gcgctggggg tgatccggat tcgggaagat gcgaaccgtc ttttcagagg
     3301 tcggctcgcc gatcttcttc atggccacga ggccgcagaa ggcgccgcac atgcagcagg
     3361 cgagcggatg acggctccag ccctggtgct cgagctcctt gagctgtgct tcgatgggcc
     3421 acatggcgcc ggccgcgccg gtcgtggcga cggcagccgc accgccgagc ttgatcagcc
     3481 cgcgacgcga cattcgaagg tcgacaatgg tgtctgccat tcgttgttct ccttggtgtt
     3541 ttgtacgaac ggctcccctg aacggggcgg gagccgcaca gaaacagatt ctgtccgcta
     3601 tgcgggacgg gagggagccg ccttcggtac tacccctatt ggatatttgt ccatactgcc
     3661 ctaccccgtt attccagata taaacagcga atcagcctga aatcggtgaa aaatccccaa
     3721 aaacaccatg gttttgcctg aaaacgggcg tcctgcaggt gtttataccc aaaaccaccc
     3781 tttcaatccg agctaaccct tatacacgaa acgccctatg gcggcaatag cattcaccta
     3841 tttacctagt tccaaagaac taggcggccg tttcccctcc tcctccacct cctccttttg
     3901 gcgaagtttc gggggcgcgg gttagaatcg aaccgtccct cttctctcct tcccgtcatt
     3961 ctcctcaggc acgatgtccg gtcccttatc cagagccctt gcgggcctcc ttttcgtcct
     4021 catgctcgtc cccggcgtct ctgcggcgtc cgacgacggc atgctcgtgc ccgacagcgc
     4081 ccacgcggcc gccgagtcgg agactgcgtc cgtcaagcgc ctcaggcttg cgatcgtcaa
     4141 ctcctacggc atgtcgatca tggacgacta ctacagccgc acgatcgagg ccgtgaagga
     4201 gaagctctct ccgatcgaga tcgacgtgaa gatctacggt cccgacagct ttctgaaggc
     4261 ggccgaaaag gaaagcttcg acatgtcgat cgcctcgagc ggcctcacga gcctcatgat
     4321 cgagcgcacg ggcgggctgc cgcttgcggc cgtcgtctcg gacgaggcgc ccgaccccaa
     4381 ctacgccaac ggcgcggcga tcatcgtgcg cgccgaccgt cccgaactca ggacgctcga
     4441 ggacctgcgc ggcaggagcg tcgccatcat gagccgcacc gccttcgcgg gctggcagat
     4501 tcccgcctcc gagatggtcc gcaaggggct cgaccccgag gtcttcttct ccgagatccg
     4561 cgtctcgggc tatccgatga cgcgcatcgt gcaggaggtg aagtcgggcg aggtggacgt
     4621 gggcttcgtg gcgacgtgcc tgctcgagcg catggcccgc gccgggcaga tcgaccagaa
     4681 ggacttccgc gtgatcggcg agcacgccga caggctcgtc gcctgccgcc actcgaccga
     4741 gctctatccg aactggttct tctcgatcaa gccctcggtc cccacgtccg tcgcccgaac
     4801 gctcaccgcc acgctgctcg acgtgccggc cgcacccgac ggcacgcagt ggacgatctc
     4861 gaacgacaac cgacgcctct tcgaggtgtt ccgcgtcctg caggtgccct acggcaatcc
     4921 cgacgacttc gggcgcttcg tgaggcgcat cctgccctgg gtcgtgggcg cgggcggcgt
     4981 gctcctcatc ctgctcctca acacgcttct tcttgcgcgc ctggcctcac accgcgcacg
     5041 cgcgctcgag aaggccatgg acgaaaagct tcgcgccgag ctcgccgccc agcagaacgc
     5101 cgtccagatc gagagcctga cgcgcgcgag cgccgtgggc cttctttcga gcatggtcgc
     5161 gcacgaactc aagcagcccc tcaccgtcat cggcaactat gccggcagcc tgcgaagacg
     5221 cctgagccgc ggagaagttc cacacgagac gctgctcgcc tccatcaccg agatcgagga
     5281 ctcggggacg cgtgccgccg ccatcatcga ccgcgtgcgc agctacggac aggtgcgcac
     5341 acgctcgctt gccgccgtcg acctatcgga cctcgtcaag actgccgtcg cccgctggcg
     5401 ccgccactct gcgcaccgcg tcccctgcac gctcgacctc gaaccgggcc tcaccgttga
     5461 ggccgaccgc cttgagattg agctcgccgt gctcaacatc gtgaagaacg cggccgccgc
     5521 cgtgaaggac cagcccgacg ccgccatcga ggtgacgctc aaggcagacg gcgcctacgc
     5581 cagactctgc atcgccgaca acgggcccga gctctccgac gaggagatcg cgcgtcttgg
     5641 cgacatcggc tacaccacca ctgcgggcgg cctcggactc ggcatcgcca tcgtccactc
     5701 actcctcgag gcccacggcg gctcgctcgc ctaccgccgc aggaccgacc gacctcggcg
     5761 gccgcggcct cgtctgcacc gtgaggctcc cgctctccgg cgcccccgag gacaggaccg
     5821 ccgcttgagc cgcaggcgga ttccgcccct ttgtttccaa ttgccgcgct tcccgtgcgg
     5881 gatccgccaa aatgcgtaga attgacggag ccgatcccat gacgacggga aatttcgtat
     5941 gagagcgaag cagtcgtcga gcgccgacag accaaactgc ggcggcgccg agatggtccc
     6001 cggacattgt tccggcagcc ataggaccgg cggacggggc cgcgggcaga gactgcacgc
     6061 ggccccgtct tcatttcaga ggcatcaaaa aagcgggcgc cccttggcgc ccgcctccag
     6121 acaacccgac tgcgggtcgg ttcaaggact ttcgtccgta tccgtcatca ttcgatgccg
     6181 ttcatctttc tggcaaaacc cgtgtagaca ttgaaggcat tgggcatgct cgactcgtcc
     6241 tgaatgtcga agttctgacg atggtgtccg tgatggttgc agccccaaag gatgaaggca
     6301 gcctgaccac ccttttgaat gacgcggcga atgaagcagg cgcaatcctc ggagcccgag
     6361 ggggcatgaa tgcgcggcag catcgtcgta ttcggcaccg ttttcatcac ctcctcaacc
     6421 ttgtcgaaaa gctcgggact ctgaaggagg gtcgtggact cacccgccat cgtcacggtc
     6481 gacttgacgc gataggcctt gtcgacaccc gaaaaaatgt cgtaaacgta gtcgcgcatg
     6541 tagccgttga cctcctccgt ctcgcctcga acctccatct gaagtctggc atgggcagga
     6601 atcacgttgc ggccctcgcc cgcatggagc gtgccgaccg agacacgggt ggtgccgtca
     6661 ccgttgcgcg gaatgccctg aagcatcatc gaagccgcac aggccgccat cagggcgtta
     6721 tgcccgagct gcggcgcgtt gcccgcatgt gcaggctggc cctcgatgtc gatgtcgaac
     6781 ttcgttgagg cgaggaagcc gccgtccatg acgccgatct caccgagttt cgcgagaccg
     6841 cccacatggc cgctgatgaa atagtccacg tcatcaacga cgccggccgc agccatcgca
     6901 cgcgcgcccc gcacaccttc ttcggccggc tgatagatga gtttgaaccg accacagagc
     6961 tcgttcttgt gatccacaag ccagcgcgcc acctcaaggc caacggccgt atggccgtca
     7021 tgaccacagg catgcatgaa acccggacgt tcggatgcaa agccaagcct gttgggcaga
     7081 tgcgcctcat cgtccgtctc gcgaacgagc acgcagtcca tgtctgcgcg gaaagccgtc
     7141 gtcggccctg gcctgcctgt atcaagcacc gccaccgcgc ccgtatagcc ctcggtctcg
     7201 tccaggaagg attgcggcac gccgtgctta agagcacgga ccatgccgtc cttcacgagc
     7261 tgcggatccc gtccgagaac ctccgactca ttgatgttgg ccttcccgac cgtgactgga
     7321 aggccgagat tcctgagaaa actcacgacg aagtacgtcg tctcgaattc cgtccagccc
     7381 tcttccggtc gcttgtgcag ctcgcggcgg ttgcggacca tttcctcgta atacttggtt
     7441 ttgtcttcca tgatggactc ctgaagttct caagcagagg ccttatctgc aacaggcaga
     7501 gcacagtgct tcaatcttat ggcaatcagc gcgcagacga tggctacgac ggcggctgcg
     7561 acgcaaacga tgtacgcgcg gctgaagtcg ccgcctgcat ttgcccagcg gatcagcagg
     7621 gggcctgtca ggccgcccac ggaatagccg aacatcatca atgaaaaatt gacgctgttg
     7681 tgcttcggac cgtactcgtc agcgacgagg ctcggataag tgccgatgaa ggcaccgaaa
     7741 catgtcccga tcccgacgat gcccgcaaaa aaaaggcctg tatcgccatg ccccgcagac
     7801 accagcatgc caagccccgc cagtgccacg accagcacga gcaccagcgt cggaatgcgg
     7861 ccgatcctgt cggagagcgt gcccgagaga aagcgacctg aagtgttggc gatcgaaatg
     7921 acggaaacgc ccagagccgc aagcccggct cccgctccca cctgctcctg agcaatggcc
     7981 gaaatgttcg aaagcagcat gagtcccatc atcgcggagg acgtaaagag aatgaacagc
     8041 ggataaaaga gcggcgttct caccatctcg agccagttca tgctcggagc ctcg
//
LOCUS       DBMDTI010000075         8007 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2435_length_8007_cov_2.263218, whole genome shotgun sequence.
ACCESSION   DBMDTI010000075 DBMDTI010000000
VERSION     DBMDTI010000075.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2435_length_8007_cov_2.263218"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="ACZPB6_05045"
     CDS             <1..1012
                     /locus_tag="ACZPB6_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter family
                     protein"
                     /protein_id="HIR1183872.1"
                     /translation="LLTILSGTGFTTMSILNVIQEVAKQNGVRPSQPLTSAVVASQIA
                     ISASPISAATAAMWVVVETMGVSFGQVLCVILPAGLFGAAVASLISAFQGVDLEKDPI
                     FQARMKAGLVAMSSQEQRDAPLPKGAKLSVVIFLLSVVFIVAMLLFKKEIGHTLGSRD
                     IIVITMLFISMLMYWFCDVELTKIKNSSIFKGGYESLVVILGICWLASTVIGIYIPTI
                     KEQATVLLGQYPGLLALAFFCVSALLFSQGATSALLVPVAASLGCDAPMILACFVAVS
                     GIFVTNVYPTSAFAISCDDTGSFMGKWSGTYVINHPFFLPGCLGLVAAIPFGFLMAGL
                     VF"
     regulatory      1139..1268
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1458..2072
                     /gene="ribB"
                     /locus_tag="ACZPB6_05050"
     CDS             1458..2072
                     /gene="ribB"
                     /locus_tag="ACZPB6_05050"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HIR1183873.1"
                     /translation="MTHAFDTIEKALEDLRAGKIILVTDDPDRENEGDFICAGEFATP
                     ENINFMAVHGRGLICMPMSGELCDRLGLAPMVAKNTDNHETAFTVSIDHVETTTGISA
                     FERSHTALAALRPDARPEDFRRPGHMFPLRAKAGGVLVRNGHTEATVDLCRLAGLAPC
                     GLCCEIMADDGTMMRTPELIEFARRHDMTFITIADLQAWIRARA"
     gene            2280..3551
                     /locus_tag="ACZPB6_05055"
     CDS             2280..3551
                     /locus_tag="ACZPB6_05055"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1183874.1"
                     /translation="MKLIKKSKKLNNVLYDIRGPIVDRAKEMEAQGQQLIKLNIGNLA
                     SFDFQVPEEIQRDMILNLPNSAGYSDSKGIFAARKAVMHYTQELGIRGVTLDDIYLGN
                     GASDLISLATNALLDEGDELLIPMPDYPLWTATTSLSGGTPVHYLCDEENGWQPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYPKETLLKLVDIAREFGLIIFADEVYDKILYEDA
                     KHIAIASLSTDVLTITFNSLSKAYRACGYRAGWMIISGNKLPARDFIEGLNMLANMKL
                     CANVPGQWAIQTALGGYQSINDLVCEGGRLRVQRDLAWELLTQIPGVTCVKPQGSLYM
                     FPRLDPKIYPIENDRQFFKELLEATRVLLVQGTGFNWPHPDHFRVVFLPHEPQLREAI
                     SRIAEYLAAWRAAHPVKEEPATPRKKEGAAE"
     gene            3630..4136
                     /locus_tag="ACZPB6_05060"
     CDS             3630..4136
                     /locus_tag="ACZPB6_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015509.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1183875.1"
                     /translation="MSVPQQRHAEPSQTLRKVLTNPLIIATVAVLLVKASGLTVPQTA
                     TTFLAHLGNDSLAMGLLCIGAGLRLHDFRDHLALITTSVVCRLLAVPAIAWGAVTLAG
                     LDPVEAGVAILFAALPTAQSCYVMTASMKGDAPSVANVTTAQTLAAMATLPFWISTVL
                     IGLSPIAG"
     gene            complement(4223..5125)
                     /locus_tag="ACZPB6_05065"
     CDS             complement(4223..5125)
                     /locus_tag="ACZPB6_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183876.1"
                     /translation="MTVMHTEAERLAMIREALERLTAAATGLFEDWQQARSFLPSGCR
                     KLMEDLTFEQASWLGRFEREAGEGTSFSALESFEGFAANVRRLVSEHAASPLALVGEV
                     FESDDEERLFFRRWKNFLDAGIGTIARSFVIDDIIFAGQIAASMRLVGLLRRITAEWA
                     HLSPEHRHQLGIDFRENAQALSDAYGTASSADFILMKDRFADRLKGEFEGFLEAADHP
                     VDETDRDAVIFRAASERFSKGVIATVKSVVTQCERGGPEAVRAFGLTLDAGRMFLELP
                     GGDETAEYCRRTLLPRLERLLESV"
     gene            complement(5462..6973)
                     /locus_tag="ACZPB6_05070"
     CDS             complement(5462..6973)
                     /locus_tag="ACZPB6_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HIR1183877.1"
                     /translation="MTDLSRRHLIQGAVAAGAALAGSTVAVASPKQKWDEVVDVIVIG
                     SGFAGLAAAIEAKKAGANVVVLEKMATFGGNSIINGGILTATGCPQQKLHKIEDSVEL
                     LEKDILTAGNYMNYSSKVRYLAEHALSNYEWTVKELGVQYLPDAIGQEGGHSVPRYVT
                     TTNGSGSGIVTKELDKCKELGIPLRNRVFVEHIIRGDNGVEGVQVREGYRFPKADSGK
                     VKTIRATKGVVLCYGGFSADVKFRMFQDPKLNETLDTTNQPGATSEMWRETSAIGALQ
                     VQNDWIQCGPWGCPREKGMGIGWQFNQTAAAEYGLWVNAEGKRFVNELANRKVRADAI
                     MVEQANGKKCFAICNEPNVQPLKKQRPGFLEKMLERKIVEKYDSLEALAKAAGIKADA
                     LKAQVEEFNKVVKAKADPVWSRYINNDQVPLVEGPWYVCELQPKVHHCMGGLVTDDNC
                     EVLDVRSDKPIGHLFAAGEATGGVHGAVRLGSVAILDCLVFGRLAGQKVAAIK"
     gene            7698..>8007
                     /locus_tag="ACZPB6_05075"
     CDS             7698..>8007
                     /locus_tag="ACZPB6_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR1183878.1"
                     /translation="MLIGVLLGIAAGALWGLIYIAPLLLPQYNPVLVALGRFIAFGLV
                     SLPFMFFMKEDLKRFTKPDIIEAFRLPFFGNVVFYSLITVCIRMSGAPLAGMFMAVIP
                     V"
BASE COUNT         1392 a         2751 c         2300 g         1564 t
ORIGIN      
        1 ccttctgacg atcctctccg gcacgggctt cacgacgatg tccatcctga acgtgattca
       61 ggaagtggcc aagcagaacg gcgttcgtcc gtcccagccg ctgacctccg ccgtcgtcgc
      121 ctcccagatc gccatttccg cctccccgat ttcggccgcc accgccgcca tgtgggttgt
      181 cgttgaaacg atgggcgtct ccttcggcca ggtcctctgc gtgattctgc cggccggcct
      241 cttcggcgcc gccgtcgctt cgctcatctc cgccttccag ggcgtcgacc ttgaaaagga
      301 cccgatcttc caggcccgca tgaaggccgg tctcgtcgcc atgtcctctc aggaacagcg
      361 tgacgctccg cttccgaagg gcgccaagct ctccgtcgtg atcttcctcc tctccgtggt
      421 gttcatcgtt gccatgctcc tcttcaagaa ggaaatcggc cacaccctcg gttcccgcga
      481 catcatcgtc atcacgatgc tcttcatctc gatgctgatg tactggttct gcgacgtcga
      541 gctcaccaag atcaagaact cctcgatctt caagggcggt tatgaatccc tcgtcgtgat
      601 tctcggcatc tgctggctcg cctccacggt catcggcatc tacatcccga cgattaagga
      661 acaggccact gtcctcctcg gccagtaccc gggcctcctc gccctcgcct tcttctgcgt
      721 gtccgctctg ctcttctcgc agggtgccac ctcggccctg ctcgtcccgg ttgccgccag
      781 cctcggttgc gacgccccga tgatcctcgc ctgcttcgtg gccgtctcgg gcatcttcgt
      841 cacgaacgtc tacccgacct ccgccttcgc catctcctgc gacgacacgg gctccttcat
      901 gggcaagtgg tccggcacgt acgtgatcaa ccatccgttc ttcctcccgg gctgccttgg
      961 cctcgtcgcc gccatcccgt tcggcttcct catggccggc ctcgtgttct aacacgcggt
     1021 ttcgaacaac ggaatccaag cctccaacgg gcgttcacct tcgggcgaac gcccgttttc
     1081 gtttccgcga acgcccgttt tcttgacatc gtcccttccc ttccgtataa attagctctg
     1141 caatcttcag ggcagggtga aagtccctac cggcggtaca gcccgcgagc gcctctccct
     1201 caggggaagc gcatgatcag gtgaaactcc tgggccgacg gtcacagtcc ggatgtaaga
     1261 agatgcaggt caaaggcgga cgcgtgcctg cgtccggcat gcgcgtgccc gcgcctgcgc
     1321 cctttgccgt cgccctgaac gttcggccaa caccgggttc gggtcgcctt cgtctcttcc
     1381 ttgagcggaa gctccccatc ccgccttcaa tcctctagag aaccgaagcc cttggcttcg
     1441 gacaaggaag acacgtcatg acccatgcgt tcgacaccat tgaaaaggcg cttgaagacc
     1501 ttcgcgccgg caagatcatc ctcgtcaccg acgatcccga tcgtgaaaac gaaggcgact
     1561 tcatctgtgc gggcgagttt gccacgcccg agaacatcaa cttcatggcc gtccacggcc
     1621 gcggcctcat ctgcatgccc atgtcgggcg agctctgcga caggctcggc ctggcgccga
     1681 tggtcgcgaa gaacaccgac aaccacgaga ccgccttcac ggtgagcatc gaccacgtcg
     1741 agacgacgac gggcatctcc gccttcgagc gctcccacac ggcgctcgcc gcccttcgcc
     1801 ccgacgcccg tcccgaggac ttccgccgcc ccggccacat gttcccgctg cgcgccaagg
     1861 cgggcggcgt gctcgtgcgc aacggccaca ccgaagccac cgtcgacctg tgccgcctcg
     1921 cgggcctcgc gccctgcggc ctgtgctgcg agatcatggc cgacgacggc acgatgatgc
     1981 gcacgcccga actcatcgag ttcgcccgca ggcacgacat gaccttcatc acgatcgccg
     2041 acctccaggc ctggatccgc gcccgcgcct aaattctgga cactcacgac gcaccgcgtc
     2101 tactcccggc ttcacgacgg cccgccgttt tcaacgaaac ggcgggccga actgcatcag
     2161 gatgcaagca aaacgccgcc ttcacgcaca cggcgtcgcc tttgcgcgta cactttgaag
     2221 ttcgacgccg ccattgacgg gcggcctttc ccccgcttga aactccgccg gaaaacctca
     2281 tgaagctcat caagaaatcc aagaagctca acaacgtcct ctacgacatc cgcggcccga
     2341 tcgtcgaccg cgccaaggag atggaggccc agggccagca gctcatcaag ctcaacatcg
     2401 gcaatctcgc ctcgttcgac ttccaggtcc ccgaggaaat ccagcgagac atgatcctca
     2461 acctgcccaa ctccgcgggc tactccgaca gcaagggcat cttcgccgcc cgcaaggccg
     2521 tgatgcacta cacgcaggag ctcggcatca ggggcgtgac gctcgacgac atttatctcg
     2581 gcaacggcgc ctcggacctc atctcgctcg cgacgaacgc gctgctcgac gaaggggacg
     2641 aactcctcat cccgatgccc gactatccgc tctggacagc caccacgagc ctctcgggcg
     2701 gcacccccgt gcactacctc tgcgacgagg agaacggttg gcagcccgat cttgacgaca
     2761 tccgcgccaa gatcaccccg aggacccgcg gcatcgtgat catcaacccg aacaacccga
     2821 cgggcgccgt ctatccgaag gagacgcttc tcaagctcgt cgacatcgcc cgcgagttcg
     2881 gcctcatcat cttcgccgac gaggtctacg acaagatcct ctacgaggat gcgaagcaca
     2941 tcgccatcgc gagcctctcc accgacgtcc tcacgatcac gttcaattcg ctctccaagg
     3001 cctaccgcgc ctgcggctac cgtgcgggct ggatgatcat ctcgggcaac aagctgcccg
     3061 cccgcgactt cattgagggc ctcaacatgc tcgccaacat gaagctctgc gccaacgtgc
     3121 cgggccagtg ggcgatccag accgcgctcg gcggctacca gtcgatcaac gacctcgtct
     3181 gcgagggcgg ccgcctgcgc gttcagcgcg acctcgcctg ggagctcctc acgcagattc
     3241 cgggcgtcac ctgcgtcaag ccccagggct cgctctacat gttcccgagg ctcgatccga
     3301 agatctaccc gatcgagaac gaccgccagt tcttcaagga gctccttgaa gccacgcgcg
     3361 tcctgctcgt gcagggcacg ggcttcaact ggccgcatcc cgatcacttc cgcgtcgtgt
     3421 tcctgccgca cgagccccag ctgcgcgagg ccatcagccg cattgccgaa tacctcgccg
     3481 cctggcgcgc cgcacatccc gtgaaggaag aacccgccac accgaggaag aaggagggcg
     3541 ccgccgaata agcgctgttc ggcagcttcc cgcctgtcca tgcagcggcc tgaaggccat
     3601 gatgtcttca ggccgctttt tctcatccta tttccgtacc tcaacagcgc catgcagagc
     3661 cttctcagac gcttcgcaag gtgctgacca atccgctcat catcgcgacc gtggcggtcc
     3721 ttctcgtcaa ggcatcgggc ctcacggtcc cgcagaccgc cacgaccttc ctcgcgcatc
     3781 tgggcaacga ctcgctggcg atgggactcc tctgcatcgg cgcgggcctc aggctccatg
     3841 acttcaggga tcacctcgcg ctcatcacga catccgtcgt ctgccgcctg cttgcggtcc
     3901 ccgccatcgc ctggggtgcc gtcacgctcg cgggcctcga ccccgtggag gcgggcgtcg
     3961 cgatcctctt tgcggcgctt cccaccgcac agagctgcta cgtgatgacg gcctcgatga
     4021 agggcgacgc cccgtcggtc gccaacgtca cgaccgccca gacgctcgcc gccatggcga
     4081 cgctcccctt ctggatctcg acggtcctca tcggcctctc gcccatcgcg ggctgagacc
     4141 ggaatcaggg aaaggcagat gcacgaacgt ccgcaggacc gcgtgtcctt gcgggcgtct
     4201 cattgttcga tccgggatga ggtcagaccg actcgaggag tcgctcgagc ctcggaagaa
     4261 gcgtgcggcg gcagtattct gccgtctcgt cgccgcccgg gagctcgagg aacatgcggc
     4321 ctgcgtcgag cgtgagcccg aaggcgcgca cggcctcggg gccgccgcgc tcgcactgcg
     4381 tgaccacgct cttgacggtg gcgataacgc ccttggagaa gcgttcggaa gcggcgcgga
     4441 agatcacggc gtccctgtcg gtctcgtcga cggggtgatc ggccgcctcg agaaagccct
     4501 cgaactcccc cttgaggcgg tcggcaaagc ggtccttcat cagtatgaag tcggcgctcg
     4561 aggcggtgcc gtaggcgtcg ctcagcgcct gcgcgttttc gcgaaagtcg atgccgagct
     4621 ggtggcgatg ctcgggcgag agatgcgccc actcggccgt gatgcggcgc agcaggccca
     4681 cgagacgcat gctcgcggcg atctggcccg caaagatgat gtcgtcgatc acgaagctgc
     4741 gcgcgatcgt gccgatgccc gcgtcaagga agttcttcca gcggcggaag aagaggcgct
     4801 cctcgtcgtc gctctcaaag acttcgccca cgagcgcaag cgggctcgcg gcgtgctcgg
     4861 agacgagcct gcgcacgttg gcggcaaagc cctcgaagct ctcgagcgcc gaaaaggacg
     4921 tgccctcgcc cgcctcgcgc tcaaagcgcc caagccagga ggcctgctcg aacgtcaggt
     4981 cctccatcag cttcctgcag cccgacggga ggaagctcct cgcctgctgc cagtcctcga
     5041 aaaggcccgt cgcggccgcc gtgaggcgct cgagcgcctc cctgatcatc gccaggcgct
     5101 cggcctcggt gtgcatcaca gtcatgttgt gtcgtcttct cattgttgtt gtgacgtcat
     5161 gcggcgcgcc tcgcttttca caggatttcc ctgcgcacgg cgggcctttc gccccgccgt
     5221 tccttttttt cgcgcttgca tgagaatgtt tcccaatagt ttacctcaat caaggcatat
     5281 ctgccctacc caagagacta ttgatttttc gaacgacgga ttcggcggga ccgttcggag
     5341 gcgttcaggg agacgtaaat gaagagcgcc cggaggcctg ggaccttcgg gcgcttcagg
     5401 cgtcctgctg cggcgcagga cggggttcag ggggctccgc cctgaggcgg agcgtcaggc
     5461 cttatttgat ggcggcgacc ttctggccgg cgaggcggcc gaagacgagg cagtcaagga
     5521 tggccacgga gccgaggcgc actgcaccgt gcacgccgcc cgtcgcttcg ccggcggcga
     5581 agagatggcc gatcggcttg tcggagcgga cgtcgaggac ttcgcagttg tcgtccgtga
     5641 cgaggccgcc catgcagtga tggaccttgg gctggagttc gcagacgtac cacggacctt
     5701 cgacgagcgg gacctggtcg ttgttgatgt agcggctcca gaccgggtcg gccttggcct
     5761 tcacgacctt gttgaattcc tcgacctgtg ccttgagggc gtcggccttg atgccggcgg
     5821 ccttggcgag ggcttcgagc gagtcgtact tctcgacgat cttgcgttcg agcatttttt
     5881 caaggaagcc cgggcgctgc ttcttgagcg gctgcacgtt gggttcgttg cagatggcga
     5941 agcacttctt gccgttggcc tgttcgacca tgatggcgtc ggcgcggacc ttgcggttgg
     6001 cgagctcgtt gacgaagcgc ttgccttcgg cgttgaccca gaggccgtat tcggcagcgg
     6061 ccgtctggtt gaactgccag ccgatgccca tgcccttttc acgcgggcaa ccccacgggc
     6121 cacactggat ccagtcgttc tggacctgga gggcgccgat ggcggacgtt tcacgccaca
     6181 tttcggacgt ggcgcccggc tggttcgtcg tatcgagcgt ttcgttgagc ttcggatcct
     6241 ggaacatgcg gaacttcacg tcggcggaga agccgccgta gcagagcacc acgcccttcg
     6301 tcgcgcggat cgtcttcacc ttgccagagt cggccttcgg gaagcggtag ccttcgcgga
     6361 cctgaacgcc ctcgacgccg ttgtcaccgc ggatgatgtg ttcgacgaac acgcggttgc
     6421 gcagcggaat gccaagttcc ttgcacttgt cgagttcctt cgtaacgatg cccgaacccg
     6481 agccgttcgt cgtcgtgacg tagcgcggaa cggagtgacc gccttcctgg ccgatggcgt
     6541 cgggcaggta ctgcacgccg agttccttga cggtccattc atagttggag agcgcatgct
     6601 cggccagata gcggaccttg ctcgagtagt tcatgtagtt gccggccgtg agaatgtcct
     6661 tctcaagcag ctcgaccgag tcctcgatct tgtggagctt ctgctgcggg cagcccgtgg
     6721 cggtcagaat gccgccgttg atgatcgagt tgccgccgaa cgtggccatt ttttcaagaa
     6781 cgacgacgtt cgcgcccgcc ttcttcgcct caatggcggc ggcaagaccg gcaaaacccg
     6841 agccgatgac gatcacgtcg acgacttcgt cccacttctg cttcggagag gcgacggcga
     6901 cggtggagcc tgcaagagcc gcaccggcgg cgactgcacc ctggatgaga tgacggcggg
     6961 aaagatcggt catggtaaat tcctcctcaa actgactcca gacgagccga cttctcctga
     7021 ggccgcgtcc ggagattccg cccttcgccc cgacgggacg cgggcgagca tagggcgcac
     7081 tttgtcgccc tccgttatta tcttactatc gacaggcgca atgcgggaga gaacaaaccc
     7141 gcatccgaat cacctcccac tgaattcccg ttcaggccta tacgtcacta ccacagttct
     7201 tgacaggaca tggaaagacg acgccgcagg cgttttgacc cctcaacgcc cctttcctca
     7261 cggtaaccga cgttacactt tgaaataagg ttgcattttc gactcatgat tacgtatttt
     7321 tactgagttt ttctttgcac ttttccgcca aaaatcagca gaatcccctc gtacctaggg
     7381 attacgtgaa gtacctaagg acttccccta aatgcatctc gcgtatttcc cgcatttttg
     7441 ttagcatgag gctgaccaaa acaagcgcat tgaacagccg ttcagaccag aagaaggctg
     7501 ttgcggattg aggcggtcgc gccgggaaaa gataccttcc cggcagccca gcggggaagc
     7561 gtcaggcggg ttccgaaagg agcgcgggcg cgcccacccg ggtcaagcac gggcggcgcc
     7621 atccctttca caccagtcgc ccgttacgga ccgcgtccgg gtgaaagacg gacgcaagaa
     7681 ccggtaatcg tcaacccatg ctcatcggcg ttctgcttgg catcgctgcg ggtgctctct
     7741 ggggcctcat ttacattgcg ccccttcttc taccgcagta caaccccgtt ctcgtcgccc
     7801 tcgggcgatt catcgccttc ggcctcgtgt cgctgccctt catgttcttc atgaaggagg
     7861 acctcaagcg attcacgaag cccgacatca tcgaagcctt ccggcttccc ttcttcggca
     7921 acgtcgtctt ctacagcctg atcaccgtct gcatccgcat gtcgggcgcg ccgcttgccg
     7981 gcatgttcat ggccgtcatt cccgtgc
//
LOCUS       DBMDTI010000076         7993 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2439_length_7993_cov_2.509757, whole genome shotgun sequence.
ACCESSION   DBMDTI010000076 DBMDTI010000000
VERSION     DBMDTI010000076.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2439_length_7993_cov_2.509757"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            79..1107
                     /locus_tag="ACZPB6_05080"
     CDS             79..1107
                     /locus_tag="ACZPB6_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR1183879.1"
                     /translation="MIEQTQAQQTQAQAPKRPASSVPLLVGGAFFAGIAAFLGWGVWE
                     ATHPAPVPLQGMVDARTISVSAKVPGRLAELKVREGDVLKAGDTVAVIAIPEIEAKLV
                     QVKAQERAAQAREDLANTGARVQEKDAARADWERAKAALTLASKTYERVDALYREGLI
                     PAQRHDEATAQLAAARKVTEAAAAKLSAVDEGARVEDKTAAKALVDQARGGVSEVSSL
                     ASESIVKAPAAGEVTRIVMEEGEVAPAGFPLVLVTDLSDKWVVFNIREDELPGVEVGT
                     VLKGFIPAVNRTVDLKVNWINPRGEYAVWRATRQSTGYDLRTFEVRARPVEELPALRP
                     GMTVIVER"
     gene            1118..2368
                     /locus_tag="ACZPB6_05085"
     CDS             1118..2368
                     /locus_tag="ACZPB6_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024110.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1183880.1"
                     /translation="MKHWFEGLVNTARIECRYTRRHPQQWVICALLPVMWLLFVANTF
                     GTGLMTKLPVGLVNEDGGPLARELVMVLDAVPSLGLRGFETATEAEVALSRAETYGTI
                     VIPRDFTKDSLNGRGSTLELIINKSYYAVGTILEVDVKTALAAVMKSAGTIRMTAARG
                     GSLKAESNNLRIASPEVYFLGNTGFNFSAYLLPTLIPGLIALAAGLTFSGVLVREWRD
                     GGATRLLAAANDFASAAILGKLLPWFVFYVVLGLCWVFGLTGVLGWTAAGSVGVWIGA
                     TVMLIAAMAAIAILFTAVSLSWPIAVSALICFAAPSFPFTGFSYPLESMTPGAAFFGQ
                     LLPLTHYLAVQGECWILNSPLDHVFTRLGPLALLIVVPMAAGLPILGVRLRAWSSKDP
                     EKDRIRTLLVKESLITDETQGGAR"
     gene            2365..3636
                     /locus_tag="ACZPB6_05090"
     CDS             2365..3636
                     /locus_tag="ACZPB6_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024110.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1183881.1"
                     /translation="MNRNNQAEQMKAPGFWYTFLHTIKASFLSRDTILIFAGAIAFYL
                     IFYAWPYGNQQIQHVPSAVLDLDRSAAARRLVTAMDASPAISVVRITQDEGEAMEAFR
                     REEFSVLITIPKDFEKSLTRGENVTVHVLGNGAFPVKARAVQAALGGVVTDKTKLLDD
                     AAVYATGLPGTSVHSHHQAAPGLRVQYMYNEIGGYGNYTVPVVGPVIIQAVMLMAITM
                     ALGGWLVAARREPFVEAALDRPLHRGAAVWLAFTLITFLWFVYMQGFDFWWHEYGSMR
                     SVAATLGTGLLFSASVASFGMAVTILLGSNRWSSQAVVMISAPAVFISGGIWPMTNVL
                     SEPVFALSLFIPTSPAVPALLAASQDGALTESILPYWGILGIQTLFYLSTALHLVHRR
                     DWREDAVQAYSRGALRREPLVKTDRPSDWDY"
     gene            3648..4436
                     /locus_tag="ACZPB6_05095"
     CDS             3648..4436
                     /locus_tag="ACZPB6_05095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1183882.1"
                     /translation="MQFIDSHSHLNSDAFDEDRDEVIARMKAAGMAAAMVIACEDDEL
                     PKLEALLAEHPGWLFGAWALHPEFPDKPEPTVERIAEICSRPGFAAVGETGLDFYWCK
                     EPLDWQRARFRRHIEAAKAIGKPLIIHARDSEREALEILRDMHAGDVGFVMHCFCGDT
                     DTALAVVDAGGHVSFTGNLTFKRNEALRETARALPLESLLLETDCPYMAPVPKRGKRC
                     EPQYVEYVAECLAGLFGVDKAHVARQTTANAVRLFRLPIRLEEA"
     gene            4444..5214
                     /locus_tag="ACZPB6_05100"
     CDS             4444..5214
                     /locus_tag="ACZPB6_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR1183883.1"
                     /translation="MKKLAVLLAAAPLLLCAPLASSADASSAGDPGAFVLSEAEVDQM
                     ARNTHDFNQAIAAGNAADVRRLLSTGITPNIVLENGDTPLTWALRSESWDLARVLLDA
                     QGIDVNMENRHGETPLMLAVIKGREAEFDRLLKLGAKVNKTRGWTPLHYAATEGRTSM
                     MKRLIELGADVNAQTKAGITALDMAARKPSREAVMMLLRAGAYRDYCTDRGMSAADFA
                     KKAGDEELAKYLAVKTCAVTGPARPELYRQPAGITLRH"
     gene            5290..7242
                     /locus_tag="ACZPB6_05105"
     CDS             5290..7242
                     /locus_tag="ACZPB6_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1183884.1"
                     /translation="MSHTPLPLTALELLAPARDADTGIAAIDHGADAVYIGGPAFGAR
                     QSAGNTMDDIASLAEYAHRYNARVFMALNTLFTNEELPRARSLAFDAAKAGVDVLIVQ
                     DMGLMAGPLPDIELHASTQCDIRTPEKAAFLEKAGFSQMVLARELSLAEVAACRAALH
                     SARIEYFIHGALCVSYSGQCYISEAITGRSANRGACAQLCRLPYDVYTESGEQIARRS
                     HVLSLRDNNQTDTLEALIDAGVSSFKIEGRLKDLVYVKNITAWYRQQLDAIIARRPEL
                     SRTSDGVSTFSFTPDPEKSFQRSRTDYFVHGRQFAKPYELAQLESPKNTGSPVGCCER
                     VEPGEILVRPAAGVSLANGDGLTYLGDDEEIHGLAVNRADPAGRGLVRLTLRNRRELP
                     EGLRRGMTLMRNLDRSFVRELSGESAVRRIPIVMTFLVEDDALTLIVTDGRECAEAHV
                     AMDLDAPSNPERNRETLIRNLGRLGDTLYTASDIFVPEDLDVFVPASVVNMMRREAVD
                     ALTKMREEARVRPGRAPVDDEAPYPDRILGFAANVANDAAREFYERHGARVTAPAFEI
                     KPVEKADLMTCRHCIRAALKRCPKMLKAFPELLEKTPREAFRPEPLVLVNSSGERFEA
                     IFHCKADPCFMTITHADDASGSRSAG"
     gene            7279..>7993
                     /locus_tag="ACZPB6_05110"
     CDS             7279..>7993
                     /locus_tag="ACZPB6_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HIR1183885.1"
                     /translation="MTNDEEPIELLMPSAAMQPVLSDLRAALVRLGDVKTRSAPRVWD
                     NIDFEGTAANIARFTLWTEGWDEGVIPAGDVNAASDIGHRLSAELEIMSPDDLEERFR
                     VIVDGAPVTRLYRRISDAQTVSRLMKDTMALGWSGFDMLALSPAEQETMRRRRMFVDA
                     FRNDFDWTRLRALELSQSAALLEAALYVEMVQPADIERLRRRIAGEAIARCASWTAFA
                     RALLCARTFCSLRDGALSTR"
BASE COUNT         1350 a         2973 c         2387 g         1283 t
ORIGIN      
        1 tcgtcaactc ccttacccgc agcgacctcg tggtcgtgaa gtgactcaga acaacgcttt
       61 tccaccggct ggaacaaaat gatcgaacag actcaagctc agcagactca ggcgcaggca
      121 cccaagcgtc ccgcctcctc cgtcccgctt ctcgtgggag gcgccttctt cgcaggcatc
      181 gcggcttttc tcggatgggg tgtctgggag gccactcatc ccgcgcccgt gcccctgcag
      241 ggcatggtcg acgcccgcac gatctccgtc tccgccaagg tgccgggacg ccttgccgaa
      301 ctcaaggtcc gcgagggcga cgtgctcaag gcgggcgaca ccgtcgccgt gatcgccatt
      361 cctgaaatcg aggccaagct cgtacaggtg aaggcacagg aacgcgccgc ccaggcgagg
      421 gaggacctcg ccaacacggg tgcccgcgtt caggaaaagg atgcggcccg cgcagactgg
      481 gagcgcgcca aggcagctct cacccttgcg tccaagacct acgaacgcgt cgacgccctc
      541 tacagggagg gcctcatccc cgcccagcgc catgacgagg cgaccgccca gcttgcagcc
      601 gcacgcaagg tgaccgaggc cgctgccgcc aagctctccg ccgtggacga aggtgcccgc
      661 gtcgaggaca aaaccgccgc gaaggccctc gtcgaccagg caagaggcgg cgtctccgaa
      721 gtctcgagcc tcgcctccga aagcatcgtg aaggcgcccg ccgcgggcga agtcacccgc
      781 atcgtgatgg aggaaggcga agtggcaccc gcgggcttcc cgctcgtgct cgtcaccgac
      841 ctcagcgaca agtgggtcgt cttcaacatc cgcgaggacg agcttccggg cgtcgaggtg
      901 ggcacggtcc tcaagggctt catccccgcc gtcaaccgca cggtcgacct caaggtcaac
      961 tggatcaatc ctcgcggcga gtacgcagtc tggcgcgcga cgcgtcagtc gacgggctac
     1021 gacctgcgca cgttcgaggt gcgcgctcgt cccgtcgaag agcttcccgc cctgcgtccc
     1081 ggcatgaccg tcatcgtcga gcgctgagcg gcacgccatg aagcactggt ttgagggcct
     1141 cgtcaacaca gcccgcatcg aatgtcgcta cacccgcagg cacccgcagc agtgggtgat
     1201 ctgcgcactc ctgcccgtca tgtggctgct cttcgtggcg aacaccttcg gcaccggcct
     1261 catgacgaag ctccccgtgg gtctcgtcaa cgaggacggc ggcccgctcg cgcgtgagct
     1321 cgtcatggtc ctcgatgccg taccgagcct cggcctcagg ggctttgaaa ccgcgaccga
     1381 agccgaagtc gcgctctcgc gcgcagaaac ctacggcacg atcgtcattc caagggattt
     1441 cacgaaggac agcctgaacg gacgcggcag cacgctcgag ctcatcatca acaaaagcta
     1501 ctacgccgtc ggcaccattc tggaagtgga cgtgaagacg gcgctcgccg ccgtcatgaa
     1561 gagcgcgggc acgatcagga tgaccgccgc gcgcggcggc tcgctcaagg ccgagtcgaa
     1621 caacctgcgc atcgcctcgc ccgaggtcta cttcctcggc aacacaggct tcaacttcag
     1681 cgcctacctg ctgccgacgc tcattcccgg cctcatcgcg cttgccgcag gactcacctt
     1741 ctcgggcgtg ctcgtgcgcg agtggcgcga cggaggtgcg acgcgccttc ttgcggccgc
     1801 aaacgacttc gcgtccgccg caatcctagg caaactcctg ccctggttcg tcttctacgt
     1861 cgtgctcggc ctgtgctggg tgttcggcct cacgggcgtg ctcggctgga cggccgcagg
     1921 ctccgtcggc gtctggatcg gcgccacggt gatgctcatc gcggctatgg ccgcgatcgc
     1981 catcctcttc acggccgtct cgctcagctg gccgatcgcc gtctcggcgc tcatctgctt
     2041 tgccgcgccg tcctttccgt tcacgggctt ttcctatccg ctcgagtcga tgacgccggg
     2101 cgccgccttc ttcgggcagc tcctgccgct tacgcactac ctcgcggtcc agggcgagtg
     2161 ctggatcctg aattcgcccc tcgaccacgt cttcacgcgc ctcgggcccc ttgcgctcct
     2221 catcgtcgtg cccatggccg ccggccttcc gatcctcggc gtgcgcctgc gcgcctggag
     2281 ttcgaaggac cccgagaagg accgcatccg cacactcctc gtcaaggaga gcctcatcac
     2341 cgacgaaacg cagggaggcg cacgatgaac aggaacaatc aggctgagca gatgaaggcg
     2401 cccggcttct ggtacacctt cctacacacg atcaaggcct cctttctctc ccgcgacacg
     2461 atcctcatct tcgcgggcgc gatcgccttc tacctgatct tctacgcctg gccctacggc
     2521 aaccagcaga tccagcacgt cccgtcggcc gtgctcgacc ttgaccgctc ggccgccgcc
     2581 cgccgtctcg tcacggccat ggacgcctcg cccgccatca gcgtcgtgcg catcacgcag
     2641 gacgaaggcg aggcgatgga ggccttcagg cgcgaggagt tctcggttct catcacgatt
     2701 ccgaaggact tcgagaagtc cctcacccga ggcgagaacg tcaccgtgca cgtgctcggc
     2761 aacggcgcct tccccgtgaa ggcccgagcc gttcaggccg cgctgggcgg cgtcgtgacc
     2821 gacaagacga agctcctcga cgatgcggcc gtctatgcga cggggctccc cggcacgagc
     2881 gtgcactcgc accatcaggc cgcgcccggc cttcgcgtgc agtacatgta caacgagatc
     2941 ggcggctacg gcaactacac cgtgcccgtc gtgggtcccg tgatcattca ggccgtgatg
     3001 ctcatggcca tcacgatggc gctcggcggc tggctcgtcg ccgcaaggcg cgagcccttc
     3061 gtggaggccg cgctcgaccg tccgctccac aggggcgcgg ccgtctggct cgccttcacg
     3121 ctcatcacct tcctctggtt cgtctacatg caggggttcg acttctggtg gcacgagtac
     3181 ggctccatgc gcagcgttgc cgcgacgctg ggcaccggcc tgctcttctc ggcctcggtc
     3241 gcctccttcg gcatggcggt cacgatcctt ctgggctcga accgctggtc gagccaggcc
     3301 gtcgtgatga tctcggcccc cgccgtattc atctcgggcg gcatctggcc catgacgaac
     3361 gtgctgagcg agcccgtctt cgcgctctcg ctcttcattc cgacctcgcc cgcagtgccc
     3421 gcgctccttg cggcctcgca ggacggggcc ctcaccgagt ccatccttcc ctattgggga
     3481 atcctcggca ttcagacgct cttctatctc tcgacggcgc tccatctcgt gcaccgccgc
     3541 gactggcgcg aggacgccgt acaggcctac agccgcggcg ccctcaggcg cgagcccttg
     3601 gtcaagaccg accggccctc cgactgggac tattgaggca acagcacatg cagttcatcg
     3661 attcacacag ccatctcaac agcgacgcct tcgacgagga ccgcgacgag gtcatcgcgc
     3721 gcatgaaggc ggcgggcatg gcggccgcca tggtcatcgc ctgcgaggac gacgagctcc
     3781 cgaagctcga ggcgctcctc gcggagcatc cgggctggct cttcggcgcc tgggcgcttc
     3841 atcccgaatt tcccgacaag cccgagccca ccgtcgagcg gatcgccgag atctgttcgc
     3901 gcccggggtt cgccgccgtg ggcgagacgg ggctcgactt ctactggtgc aaggagccgc
     3961 ttgactggca gcgcgcacgt tttcgccgcc acatcgaggc cgcgaaggcc atcggcaagc
     4021 ccctcatcat ccacgcgcgc gacagcgagc gcgaggcgct cgagatcctg cgcgacatgc
     4081 acgcgggcga cgtcggcttc gtgatgcact gcttctgcgg cgacacggac acggcgctcg
     4141 ccgtcgtgga cgcgggcggc cacgtaagct tcacgggcaa cctcaccttc aagcgaaacg
     4201 aggcgctgcg cgagaccgcg agggcgcttc cgctcgaaag cctcctgctc gagaccgact
     4261 gtccgtacat ggcgcctgtg cccaagcgcg gcaagcgatg cgagcctcag tacgtcgaat
     4321 atgtcgcaga atgtctcgca ggacttttcg gcgttgacaa agcgcacgtc gcccgacaaa
     4381 ctacggccaa cgccgtcagg ctcttccgcc tgcccatccg tcttgaggaa gcataatcag
     4441 tctatgaaaa agctcgccgt tcttcttgcc gccgccccgc ttcttctctg cgcgcccctc
     4501 gcatcgtccg ccgacgcgtc gtccgcaggc gacccgggcg ccttcgtcct ctccgaggcc
     4561 gaggtcgatc agatggcccg caacacgcac gacttcaatc aggcgatcgc cgccggcaac
     4621 gccgccgacg tcaggcgcct tctctccacg ggcatcacgc ccaacatcgt gctcgaaaac
     4681 ggcgacacgc cgctcacctg ggccctgcgc agcgagtcgt gggatctcgc ccgcgtgctg
     4741 ctcgacgccc agggcattga cgtcaacatg gagaaccgcc acggcgagac gccgctcatg
     4801 cttgcggtga tcaagggccg cgaggctgaa ttcgacaggc tgctcaagct cggtgcgaag
     4861 gtcaacaaga cccgcggttg gacgccgctc cactacgccg ccaccgaagg ccgcacctcc
     4921 atgatgaagc gcctcatcga actcggcgcc gacgtcaacg cccagacgaa ggcgggcatc
     4981 accgcgctcg acatggccgc aaggaaaccg agccgcgagg ccgtcatgat gctccttcgc
     5041 gcaggcgcct accgcgacta ctgcaccgac aggggcatgt ccgccgcaga cttcgccaaa
     5101 aaggcgggcg acgaggagct cgccaagtac ctcgccgtca agacctgcgc cgtcacgggc
     5161 cccgccagac ccgagctcta caggcaaccc gccggcatca cgctccgtca ctgacgctcg
     5221 gcccctcatg cacaggccat cggacgcacg cccgcgcggc ctttccacga caatcccgag
     5281 gattccttcg tgtcccacac gcctcttccc ctcaccgctc ttgaacttct cgcccccgcc
     5341 cgcgacgccg acaccggcat cgccgcgatc gatcacggcg ccgacgccgt ctacatcggc
     5401 ggccctgcct tcggcgcccg ccagtccgcg ggcaacacga tggacgacat cgcgagtctc
     5461 gccgagtacg cgcaccgcta caacgcccgc gtcttcatgg cgctcaacac gctcttcacg
     5521 aacgaggagc ttccgcgcgc ccgctcgctt gccttcgacg ccgccaaggc cggagtcgac
     5581 gtcctgatcg ttcaggacat gggcctcatg gcgggcccgc tgcccgacat cgagctccat
     5641 gcctcgacgc agtgcgacat ccgcacgccc gaaaaggccg cgttcctcga gaaggcgggc
     5701 ttctcgcaga tggtgctcgc gcgcgagctc tcgctcgccg aggtcgccgc ctgccgagcc
     5761 gcgctccatt cggcgcgcat cgagtacttc atccacggcg cgctctgcgt gagctattcg
     5821 ggccagtgct acatcagcga ggcgatcacg ggacgaagcg ccaacagggg cgcctgcgcg
     5881 cagctctgcc gcctgcccta cgacgtctac acggagtcgg gcgagcagat cgcccgccgc
     5941 agccacgtgc tttcgctgcg cgacaacaac cagaccgaca ctctcgaggc cctgatcgac
     6001 gcgggcgtct cgtccttcaa gatcgagggc cgcctcaagg acctcgtcta cgtgaagaac
     6061 atcacggcct ggtaccgtca gcagctcgat gccatcatcg cgcgacgccc cgaactctcc
     6121 cgcacctccg acggcgtctc gaccttcagc ttcacgcccg accccgagaa gagcttccag
     6181 aggagccgaa ccgactactt cgtgcacggc cgtcagtttg caaagcccta cgagctcgcc
     6241 cagctcgaga gccccaagaa cacgggcagc cccgtcggct gctgcgagcg cgtcgagccg
     6301 ggcgaaatcc tcgtgcgtcc cgctgcgggc gtctcgcttg ccaacggcga cggcctcacc
     6361 tatctcggcg acgacgagga gatccacggc cttgccgtca accgtgccga tcccgccggc
     6421 aggggcctcg tcaggctcac gctgcgcaac cgccgcgaac tccccgaggg gcttcgccgc
     6481 ggcatgacgc tcatgcgcaa cctcgaccgc tccttcgtgc gcgagctctc gggcgagtcc
     6541 gccgtgcgcc gcattccgat cgtcatgacc ttcctcgtcg aggacgacgc gctcacgctc
     6601 atcgtgaccg acggccgcga atgcgccgaa gctcatgtgg cgatggatct cgacgccccc
     6661 tcgaaccccg agcgcaacag ggagacgctc atcaggaacc tcggcaggct cggcgacacg
     6721 ctctacacgg cctccgacat cttcgtgccc gaggacctcg acgtcttcgt gcccgcctcg
     6781 gttgtcaaca tgatgcgccg cgaagccgtc gacgcgctca cgaagatgcg cgaggaagcc
     6841 cgggtccgtc ccggccgcgc ccccgtcgac gacgaggcgc cctatcccga ccgcatcctc
     6901 ggcttcgccg ccaacgtcgc caacgatgcc gcgcgcgagt tctacgagcg ccacggcgcc
     6961 cgcgtgaccg cgcccgcctt cgagatcaag cccgtggaga aggcggatct catgacctgc
     7021 cgccactgca tccgcgccgc gctcaagcgc tgtccgaaga tgctcaaggc cttccccgaa
     7081 ctgctcgaga agacgccgcg cgaagccttc aggcccgagc cgctcgtcct cgtcaactcg
     7141 tcgggcgagc gcttcgaggc gattttccac tgcaaggccg atccctgctt catgacgatc
     7201 acgcacgccg acgacgcgag cggctcccgc tcagcgggat aaaatgaagg gtcgccccat
     7261 cctccggctc ccaaacccat gacgaacgac gaagaaccga tcgagctcct gatgccatcc
     7321 gccgccatgc agcccgtcct ttcggacctg cgtgccgcgc tcgtgcgcct cggcgacgtg
     7381 aagaccagat ccgcgcctcg cgtctgggac aacattgact tcgagggcac ggccgcgaac
     7441 atcgcgcgct tcacgctctg gacggaggga tgggacgaag gcgtgatccc cgcaggcgac
     7501 gtcaacgccg caagcgacat cggacaccgc ctctcggccg agcttgagat catgtcgccc
     7561 gacgacctcg aagaacgctt ccgcgtgatc gtcgacggcg cccccgtcac caggctctac
     7621 cgccgcatct ccgacgccca gaccgtcagc cgcctgatga aggacaccat ggcgctcggc
     7681 tggtcgggct tcgacatgct cgcgctctcg cccgccgagc aggagacgat gcgccgcagg
     7741 cgcatgttcg tcgacgcctt ccgcaacgac ttcgactgga cgcgcctgcg cgccctcgaa
     7801 ctctcccaga gcgcagcgct tctcgaggcc gcgctatacg tcgagatggt ccagcccgcc
     7861 gacatcgaac gcctgagacg ccgcatcgcg ggcgaggcga tcgcgcgctg cgcgagctgg
     7921 accgcattcg cccgtgcgct tctgtgcgca cgcaccttct gcagcctcag ggacggggcg
     7981 ctcagcaccc gaa
//
LOCUS       DBMDTI010000077         7960 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2452_length_7960_cov_2.352844, whole genome shotgun sequence.
ACCESSION   DBMDTI010000077 DBMDTI010000000
VERSION     DBMDTI010000077.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2452_length_7960_cov_2.352844"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="ACZPB6_05115"
     CDS             complement(<1..418)
                     /locus_tag="ACZPB6_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1183886.1"
                     /translation="MSFEIERRRIYSSGTIFRMAVPLMLSFLLEQLIGMTDVAFLGRV
                     GEVELGAAALAGVFFLMLMMFGMGYGFGVQAHMSRKNGEKAWAEIGRSFRSGVLFLTG
                     LAAVLIVLSDLAAKPFLDTACESAAVAEAADTYLYWR"
     gene            complement(572..841)
                     /locus_tag="ACZPB6_05120"
     CDS             complement(572..841)
                     /locus_tag="ACZPB6_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183887.1"
                     /translation="MAAILASFSWTILREFIMEGITYRQDIEETIERYKKRVAKNPED
                     EIAKEVLQDLEWELRLIDRKEMMDKMREEEPDRWTFFMSGPNINE"
     gene            1146..2330
                     /locus_tag="ACZPB6_05125"
     CDS             1146..2330
                     /locus_tag="ACZPB6_05125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HIR1183888.1"
                     /translation="MSKQVLLDRRGFLRGGASVAMAAVLGSSETLAAEAGEKPKIRPL
                     EGKMDYESIRKRAREMMMPRCYVCPECNGRGPCVGQVPGFGGMGASRGFQANYDSLAA
                     VQLNSRVIHGVHVPDTSIDFFGTKISMPAVAAPTGGTTYNMGGKLTEKKFVTAICGGC
                     TKAGTLGAVADGIGDPLPVFEERLQTLKSLGYKAIVGLKPRLNKDIIERMKLAEQAGV
                     VALTIDLDSAGRAARATKGQTVEPKTFEQLKELVSASPLPLLFKGIMTPDEAELCVQA
                     GAAGIVVSNHGGRTLADTPGTAAVLPRIADKVNGRCYIMVDGTVARGTDVEKYLALGA
                     QCTLVGRHFVRAAHGGLEDGVALFANKIKSELAVAMVLTGAQRVSDINRKMIVIPPEY
                     LA"
     gene            2472..3542
                     /locus_tag="ACZPB6_05130"
     CDS             2472..3542
                     /locus_tag="ACZPB6_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024138.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1183889.1"
                     /translation="MYSVEFAYNIVPSDEAGQGLSNLLIRQLAAIESGKGLQQAARSL
                     GVSYRYFWGQIEIWEERFGQKLVIREQGKRARLSPLGQKLLWAERTVMANYAVQMEKM
                     RTELNAAFAAACNPDADIVTIAGCFDPWLATLPAFLFPSHLIADLQFSTSVSGLRALA
                     DEKCDFAGFNFPRGAGRESEAARTFAPLIDPEAMCLCRFASRTQGLAVAPGNPLRISS
                     IRDVLRDGLQYAGRAVGTGTEVLLRDLCAQADLDPAGLHRISTEVSHRAVAMAVAAGR
                     ADAGLCVENAARQAGADFLPLTVEDYFLAWRRESAPAHLDKVLSTLSSDEWRSTVVRF
                     AGVSGINCGEPVDCRTEFGWWG"
     gene            complement(3663..3857)
                     /locus_tag="ACZPB6_05135"
     CDS             complement(3663..3857)
                     /locus_tag="ACZPB6_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183890.1"
                     /translation="MVVIIALLHCLPPILGALIGGREGLATGTIIGVICAFVFGALVF
                     TIPDLLGVGLGFVIGKVFTR"
     gene            complement(4280..4462)
                     /locus_tag="ACZPB6_05140"
     CDS             complement(4280..4462)
                     /locus_tag="ACZPB6_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR1183891.1"
                     /translation="MEDKDVILEAMRKAGEPLNAGKVAELTGLDRKAVDKAFAAMKKT
                     GEIVSPVRCKWEPAQK"
     gene            4908..6455
                     /locus_tag="ACZPB6_05145"
     CDS             4908..6455
                     /locus_tag="ACZPB6_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1183892.1"
                     /translation="MFKIRKTAALAALFCSSALNAWASADHMVIAMPQIPTIIEPQGI
                     NNNAIDRYVGNVFETLLKADQKTGELKPGLAESWRRLSPDTVEFKLRSGVRFHDGTPL
                     TADDVVFTFGPERFSGEKAPGRAVAWEFLGNLKEVVKVDDLTVRVTMKAPDPMIERRF
                     SARTSEIISEDGWKAAGGWEKWLKAPVGTGPYRIASFKTGNRLELTRFDGYWGKTAPA
                     AKVSFVEVPELSARVAGLRSGEFDLITEVPPDQVKPLSADGRIDVTGGAIDNIYGLVF
                     DCKSSAVMQSKELRQAILHAIDRDILVNALFAGRTSTADNFQSKTFGELYLTTAGEKL
                     YDPAKARALVKASGYKGEPIVWRIQAGYYTQELVVTQAVASMLKAAGLNVKIEVKENW
                     TQVEAPGRDRMINNASFSAYYPDPVAQLWRRLKPSSFWVQNGYVADSAEYRRFCELGE
                     KLETSVDPAERKAAWGEMLKVFSQNPWACPLYSLPMLYAKQKNVVWEASALQGSLDLS
                     AENLSFQ"
     gene            6645..>7960
                     /locus_tag="ACZPB6_05150"
     CDS             6645..>7960
                     /locus_tag="ACZPB6_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1183893.1"
                     /translation="MSNMSAETVSTCKLLDVRGLRVSFDAVDVLHGVDLTVHCGRIHA
                     LIGESGSGKSVLARSILGLAGRGARTTGSIRFLGRELVGLSEREYRAIRGADIGLVVQ
                     DAMSALNPMRTIGEQLVETIACRHPDFRSGASRASRSSRADRHDIALELLRTVGITAP
                     EKRMNAYAHQLSGGMRQRVMIALALTGSPRLLLADEPTTALDVVVQRELLQEMRALVQ
                     ARGMSMLIVTHDLNLARDVADDVSVMYAGHVLEEGPVSDVITRPAHPYTEGLLRAMPA
                     MDGTRGQLIPIKGEIPPPDKLGSGCPFASRCPRVTNACRESLGAMTAVEDGRRRTRCD
                     AALLTAAPHAPSCASRLASLPVQDTEFPVISDIRIARHCYVSRQGLLGRRRPWDVLQD
                     IDLQVRSGEVMGLVGESGCGKSTLARLALGLIEPTEGTVRFKNAPIP"
BASE COUNT         1487 a         2670 c         2294 g         1509 t
ORIGIN      
        1 cgcgccagta gagatacgtg tcggccgcct cagccaccgc cgccgactcg caggccgtat
       61 cgagaaaagg cttggccgca aggtcggaga gcacgatgag aaccgccgcg agccccgtga
      121 gaaagagcac cccggagcgg aacgaccgcc cgatctccgc ccaggccttc tcgccgttct
      181 tcctgctcat gtgcgcctgc acgccgaagc cgtaccccat gccgaacatc atgagcatga
      241 ggaagaagac gcccgcgagc gccgcggcgc cgagctccac ttcgccgaca cgcccgagaa
      301 aggcgacgtc ggtcatgccg atgagctgct cgagcaggaa gctcagcatc agcggaacgg
      361 ccatgcggaa gatggtgccg ctcgaataga tgcggcggcg ttcgatttcg aaggacatgt
      421 gcagaaagac tgaagaggcc cgaaacgcag gccctgaact gaaagtgccg tattttaccg
      481 acttgcagac tccttttcag cgcgcccgcg agcgccaaaa agtcagggcg cccgtccgaa
      541 gacaggcgct cctcatcctt tttttaggct cttactcgtt gatgttcggg ccgctcataa
      601 agaaggtcca ccggtcgggc tcttcctccc tcattttgtc catcatctct ttcctatcga
      661 tcaacctgag ctcccactcc agatcctgaa gcacttcctt cgcaatctcg tcctcgggat
      721 tcttcgccac tcgtttcttg tagcgctcga tcgtttcctc gatatcctgt cggtacgtaa
      781 tcccttccat tatgaattcc ctcaagatcg tccacgaaaa cgacgcgagt atcgcagcca
      841 tgctgccggg caatcctcac gccatcggtg ctcccgatct cttcgtcgag agctctgccc
      901 ggtcctgcgt cggacgggtg ccgcaatctg agtataggac ttgacgccct gaccgacgac
      961 atcaattcgg gcggaccaga taccgtaaat tcactcatgc tctatgccgt ccgtcaacat
     1021 atgaacacat aaacatatgt acagagaaac ataagcggat aatgggcttg accctcgaga
     1081 ggtcgttgaa tcggacacgg gcgtgtcggt gatcggcctc tctctgaaaa aaatggagaa
     1141 agaccatgtc caaacaagtt ttgcttgatc gtcgcggctt cctgcgcggc ggcgcatccg
     1201 tcgccatggc ggcggttctc ggatcctctg aaacgttggc ggctgaagcc ggtgaaaagc
     1261 cgaagattcg tccgctcgaa ggcaaaatgg attatgagtc cattcgcaag cgcgcccgtg
     1321 aaatgatgat gccgcgctgc tacgtctgtc ctgaatgcaa cggcaggggg ccttgcgtcg
     1381 gtcaggttcc cggtttcggc ggcatgggcg caagccgagg cttccaggcc aactatgact
     1441 ctctcgccgc agtgcagctc aacagccgtg tgatccatgg agtgcatgtg cccgacacgt
     1501 ccatcgactt cttcgggacg aagatctcca tgccggccgt tgccgcgccg acgggcggca
     1561 ccacctacaa catgggcggc aagctcaccg aaaagaaatt cgtcactgcc atttgcggcg
     1621 gctgcacgaa ggccggcacg ctcggtgccg tggcggacgg catcggtgat ccgctccccg
     1681 tcttcgagga acgtcttcag acgctcaagt ccctcggcta caaggccatc gtcggtctca
     1741 agccgcgtct caacaaggac atcatcgaac gcatgaagct tgccgaacag gccggtgtcg
     1801 tcgcgctcac gatcgacctt gacagtgcag gccgagccgc tcgcgccacg aagggccaga
     1861 ctgtcgaacc caagaccttc gaacagttga aggaactcgt cagtgcctcg ccgcttccgc
     1921 ttcttttcaa gggcatcatg acgcctgacg aagccgaact gtgcgttcag gcgggcgccg
     1981 ccggcatcgt cgtctccaat cacggcggcc gtacgctcgc cgacactccg gggacggcag
     2041 ccgtgctccc gcgcatcgcc gacaaggtca acggtcgctg ctacatcatg gtggacggca
     2101 cggttgcccg aggtaccgac gtcgagaagt atctggcgct cggcgcccag tgcacgctcg
     2161 tcggccgtca cttcgtccgt gctgctcatg gcggtctcga agacggcgtt gcgctctttg
     2221 ccaacaagat caagagcgaa ctcgcggtcg cgatggttct caccggcgca cagcgcgtct
     2281 ccgacatcaa tcgcaagatg atcgtcattc cgcctgagta ccttgcttga ggcgagagcc
     2341 tcatgatgct cccggcgtct gagacgcctc ggagcatctc acgcctagta acgcccggtc
     2401 cttcgatcgg gcgtttgctt ttgaggcgca aggcgggcaa tatcaaggca tcagcttaag
     2461 ggccgtactc catgtattca gtcgaattcg cttataacat cgtgccgtcc gacgaggccg
     2521 gacagggact ctccaacctt ctcatccgcc agcttgccgc gatcgaaagc ggcaagggac
     2581 ttcagcaggc cgcccgcagc cttggcgtct cctaccgcta tttctggggg cagatcgaga
     2641 tctgggagga gagattcgga cagaagctcg tcattcgcga acaaggcaaa cgcgcgcgac
     2701 tctcgccgct tggccagaag cttctctggg cggagcgaac cgttatggcg aactacgccg
     2761 ttcaaatgga gaagatgcgg accgagctca acgctgcttt tgccgcagcc tgcaaccctg
     2821 atgcggacat cgtgaccatt gccggctgct tcgatccatg gctcgcgacc ttgcccgcct
     2881 ttctctttcc ttctcatctc atcgcggacc tgcagttcag taccagcgtc tcggggctcc
     2941 gggctctggc ggacgagaag tgcgatttcg ccggcttcaa ctttccccgc ggcgccggca
     3001 gagagagtga agcggcccgt accttcgctc ctctcatcga tcccgaagcc atgtgcctct
     3061 gtcgatttgc ctcccgcact cagggactgg ccgtggcacc cggaaatccc cttcgcatct
     3121 cctccatccg ggatgtcttg cgcgacggcc ttcaatatgc aggccgcgct gtcggtaccg
     3181 ggaccgaagt ccttttgaga gatttgtgtg ctcaggcgga tctggatccg gcaggattgc
     3241 accgcatttc gacggaggtg tcgcatcgcg ccgtcgcgat ggccgtcgct gcaggacgtg
     3301 cggacgcggg cctctgcgta gaaaacgctg cacggcaggc cggcgccgac tttctgcctt
     3361 tgaccgttga ggactacttt ctggcttgga gaagagaatc ggcacctgca catctcgaca
     3421 aggtactcag cacactctcc tcggatgagt ggcgaagcac tgtcgtccga ttcgccggtg
     3481 tttcgggcat caattgcgga gagccggtcg attgccgtac tgagtttggt tggtggggat
     3541 gagagcgggc gtgatcgctt gcaggtgcaa acgggacgag agaaagccgg atgcccgcca
     3601 gataaagcct caagccgtca ataacgggag gcagtcagac gggcagcggt tgagggacaa
     3661 tttcagcggg tgaacacctt gccgatgaca aaaccgagac caacgcccag aaggtccgga
     3721 atcgtgaaca ccaatgcgcc gaagacaaag gcgcaaatca caccgatgat cgtgccggtg
     3781 gcaagtccct ctcttcctcc gatgagtgct ccgaggatgg gcggaaggca gtgaagcaat
     3841 gcaatgatga ctaccatgat gggatacctc agggcaaagc ccggcctgtg tgcttcggag
     3901 cctttggaga ccagccgatt gacctgaggt ttcgaacagt caggttttag gtggcgatat
     3961 cggaggatgg ctcgcgtgtg acagcaggca ggagcgtgct cgactatgca tgttgttttc
     4021 gaatcgcatg gatctcatgg agggaactag cgaaatcttt ttcattatag gatgccgtgc
     4081 atggaatgac tttgccggac accacacggt acggatcgat ccctcactga aaatcgatgg
     4141 atgaaagtcg ggaatggatt ttcatctcct ccgggcaggg ccatgcgaag ttgcccgcga
     4201 gcgccaaaaa gtcagggcgc ccgtccgaag acaggcgccc ctcatccttt tttttagtca
     4261 agcccatgca gggcgcggct tatttctgag ccggctccca cttgcagcgg accggcgaga
     4321 cgatttcccc cgtcttcttc atcgccgcaa aggccttgtc gaccgccttg cggtcaagcc
     4381 ccgtaagttc ggcgaccttg ccggcattga gcggttcgcc cgccttgcgc atggcttcga
     4441 gaatgacgtc cttgtcttcc atttctgtct tcctttgact tcgtagggtt cggggcggca
     4501 caccgccccc tctgtccgct cattgtccca gactttcgga aggactgtcc cgattgacat
     4561 agtgttttac aaacactcct catgatattg atggcatcaa tttgaacgat tgaacggccc
     4621 tattcctgag catccgccgc atccatccgc gaaccgaccg tccgcatcca tcgtcatcaa
     4681 aacgtcatgt ccgtgtcgtc ttcgtgaagc acaaggcctt cagaatctca cggcgacggc
     4741 gaacgtcgtg cggcccgcgc cttcagttcg tgttctccgg tgccggagcc tcagcgtgag
     4801 tcgtcaccgc attcagacct ttgttttcgg actccgtgcg gagtccggcg cgggactctc
     4861 ccttcattcg tcattcggag ccccgcgcgt tgctgcagga aatgtaaatg ttcaagatcc
     4921 gcaagaccgc cgccctcgcg gccctcttct gttccagcgc cctcaacgcc tgggcctcgg
     4981 ccgatcacat ggtgatcgcg atgccgcaga ttccgaccat catcgagccg cagggcatca
     5041 acaacaacgc gatcgaccgc tatgtcggca acgtcttcga gacgctcctc aaggcggacc
     5101 agaagacggg cgagctcaag ccgggacttg ccgaatcgtg gcgccgcctc tcacccgaca
     5161 cggtagagtt caagctgcgc tccggcgtca ggttccacga cggcacgcca ctcacggccg
     5221 atgacgtcgt cttcacgttc gggcccgagc gcttctcggg cgaaaaggcg ccgggccgcg
     5281 ccgtcgcctg ggaattcctc ggcaacctca aggaagtggt caaggtcgac gacctcaccg
     5341 ttcgcgtcac catgaaggcg cccgacccga tgatcgagcg ccgcttctcc gcgcgcacct
     5401 ccgagatcat ctccgaggac ggctggaagg ctgcgggcgg ctgggagaag tggctcaagg
     5461 cgcccgtggg cacgggtccc tacaggatcg cctccttcaa gaccggcaac cgtcttgagc
     5521 tcacgcgctt cgacggctac tggggcaaga cggcacccgc cgccaaggtg agcttcgtcg
     5581 aggtgcccga actctccgcc cgcgtcgcgg gccttcgctc gggcgaattc gacctcatca
     5641 ccgaagtccc gccggatcag gtgaagcctc tttccgccga cggtcgcatc gacgtcacgg
     5701 gcggcgccat cgacaacatc tacggcctcg tcttcgactg caagtcctcc gccgtcatgc
     5761 agtcgaagga actccgtcag gccatcctcc acgcgatcga ccgcgacatt ctcgtcaacg
     5821 cgctcttcgc gggccgcacc tccacggccg acaacttcca gtcgaagacc ttcggcgaac
     5881 tctatctcac cacggcgggc gaaaaactct acgatcccgc caaggccagg gctctcgtca
     5941 aggcctcggg ctacaagggc gagcccatcg tctggcgcat ccaggcgggc tactacacgc
     6001 aggagctcgt cgtcacgcag gccgtcgcct ccatgctcaa ggccgcaggc ctcaacgtga
     6061 agatcgaggt caaggaaaac tggacccagg tcgaggcgcc gggccgcgac cgcatgatca
     6121 acaacgcgag cttctccgcc tactatcccg atcccgtcgc ccaactctgg cgccgcctca
     6181 agccctcgag cttctgggtg cagaacggct acgtcgccga ctcggccgag tataggcgct
     6241 tctgcgagct tggcgaaaag ctcgagacga gcgtcgatcc cgccgagcgc aaggccgcct
     6301 ggggcgagat gctgaaggtc ttctcgcaga atccctgggc ctgcccgctc tactcgctcc
     6361 cgatgctcta cgccaagcag aagaacgtcg tctgggaggc gagcgccctt cagggaagcc
     6421 tcgacctctc ggcggagaac ttgtccttcc agtaatgccg gcatcctccg gcatcggatt
     6481 ccggacgaac tcccgcgcag ggcggccgtc cgggatctgg ccccgcacgc cgaacgcacg
     6541 aaagccgcgg acgcagggat cccgtcatcc cgtgcgtccg cccgcgttct ccgtcccaga
     6601 ctttccggat tcctcacgtc cctcaccttc aacacatgtc tcacatgtcc aacatgtctg
     6661 cagagactgt ctccacctgc aagcttcttg acgtgcgcgg ccttcgcgtc tcgttcgacg
     6721 ccgtcgacgt gctgcacggt gtggacctca ccgttcactg cgggcgcatc catgcgctca
     6781 tcggcgagtc gggctcgggc aagagcgtcc tcgcccgctc gatcctcggt ctggccggcc
     6841 gcggcgcccg caccacgggc tccatccgct ttctcggccg cgagctcgtc ggtctttccg
     6901 aacgcgagta ccgcgccatc cgaggggccg acatcggcct cgtcgtccag gacgccatgt
     6961 ccgcgctcaa cccgatgcgc acgatcggcg agcagctcgt cgaaaccatc gcctgccgcc
     7021 atcccgactt ccgaagcggc gcctcgcgtg cttcgcgcag ctcacgcgcc gaccgccacg
     7081 acattgcgct cgagcttctg cgcaccgtcg gcatcaccgc gcctgaaaag cgcatgaacg
     7141 cctacgccca ccagctctcg ggcggcatgc gccagcgcgt gatgatcgcg ctcgccctca
     7201 cgggcagccc caggctgctc ctcgccgacg aacccaccac ggcgctcgac gtcgtcgtgc
     7261 agcgggagct cctgcaggaa atgcgtgcgc tcgtgcaggc gcgcggcatg tcgatgctca
     7321 tcgtcacgca cgacctgaac ctcgcccgcg acgtcgccga cgacgtctcc gtcatgtacg
     7381 cgggccacgt ccttgaggaa ggccccgtct ccgacgtcat cacgcgtccc gcccatccct
     7441 acaccgaagg ccttctgagg gccatgcccg ccatggacgg cacccgcggc cagctcatcc
     7501 cgatcaaggg cgagattccg ccgcccgaca agctcggctc gggctgtccc ttcgcctcgc
     7561 gctgcccgcg cgtcacgaac gcctgccgcg aaagtctcgg cgccatgacg gccgtcgagg
     7621 acggccgcag gcgcacccgc tgcgacgccg cgctcctcac cgccgccccg catgcgcctt
     7681 cgtgcgcctc gcgccttgcg tccctgcccg ttcaggacac cgagtttccc gtcatctccg
     7741 atatccgcat cgcccgccac tgctacgtct cgaggcaggg ccttctcggc cgccgccgtc
     7801 cctgggacgt cctgcaggac atcgacctgc aggtccgctc gggcgaggtc atgggactcg
     7861 tgggcgaatc gggctgcggc aaatcgacgc tcgcgcgcct cgcgctcggc ctcatcgagc
     7921 ccaccgaagg cacagtgcgc ttcaagaacg cgccgatccc
//
LOCUS       DBMDTI010000078         7841 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2502_length_7841_cov_2.245736, whole genome shotgun sequence.
ACCESSION   DBMDTI010000078 DBMDTI010000000
VERSION     DBMDTI010000078.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2502_length_7841_cov_2.245736"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2771)
                     /locus_tag="ACZPB6_05155"
     CDS             complement(<1..2771)
                     /locus_tag="ACZPB6_05155"
                     /inference="COORDINATES: protein motif:HMM:NF024486.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HIR1183894.1"
                     /translation="MIATEAQREAIDSLLTFIEEATSLRQTAARHLSRCGRLLRLPEL
                     EAAVPGLVAGDPTGADADLVCRIEKLEMPPCPPIPESLAPYATGDWRAPEWTPDWKAL
                     PDPAEHPESAELYETLADWLRIRGSWLEARGEILPNHALFERLQDLRDHLSESNLRDE
                     CVIGTAVFTSNPDVTRAKNPVRWPLLVQPLVIELGSSRRNLPVIEVRRNGDEDPRLLA
                     EILAPFAEDGIDLKAASRFEEWLEDAGADANLAGDESLSEALEKFAASLHPDCRFMPA
                     EEELPEDAGEKPAFTLEASPVILIQPRPSGVRSAIRSIRKDIAEHRDVPAHLMEIVCP
                     DVFPTLASGLTPTLSLEAKLAATAGEDPDVLLAKPANPEQLAIAHEVEHNNVVLVQGP
                     PGTGKTHTIANLLGHFLSQGKRVLVTSHTTKALSVVKDLLPKEIQPLCVTMLGDRKDL
                     EQTSSELITRLTRLNVEDLEARIRALGLERHRLGTALADRRRRIFEQRRIEHEAVEFN
                     GRRYTLTEIAKFLREAERLQALIPGEVAEGPLPLSFRELLELYGTNGRWDAETARDLS
                     GLLPGFELLPDVDTMRGMNRRWRDVLAEDAGDVTIDERRSASGERLHAFMKGGETLLV
                     APESGISALTPAMDLSPLTETDEMKRLALSLGLADEGCREAFEQLERELAAANELALA
                     VDRASLLDNRTVVIPDAIDADAAMKAAEWFTANDPDGSTGFLGRLFNKEAREAADALA
                     GVEVDGSRPASKEAFEAVALHARLKAAVKTVARTWDSLAERADAPAFATYGNNAVRTL
                     FTRYGTSLSDTCIWWRRVFVPYIKGLLSAGIEAEGMKELLESADPLEAARRFATDVLA
                     PVHTARHREAELHALREWQATESQKLYAAAPACATARRLAAATLVDPDAWELEAERLR
                     KLLEDQPLFA"
     gene            3156..3548
                     /locus_tag="ACZPB6_05160"
     CDS             3156..3548
                     /locus_tag="ACZPB6_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183895.1"
                     /translation="MLRRNLLLGVAGVAVLGVTGCATHPVSVQQAAELPAAQLRTCIK
                     RAAHRRGWRVVTDEPGKMRIKYVKGPHVLVADVTYDNEGYMIEAVKEGSTLFNKDGTA
                     HRKVNSWTANLAVTIREEENKILLRQTN"
     gene            3575..4657
                     /locus_tag="ACZPB6_05165"
     CDS             3575..4657
                     /locus_tag="ACZPB6_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR1183896.1"
                     /translation="MKESIKKTRRTPALEALNEAHRLAVSPFVFQALCAMTDLGMMKA
                     LDEAGPEGLTLEALCEKSPCSDYAARALLEVAVRFGVAQRSDEGRFSLTNTGIFFVND
                     LHVGINFRFTRDVCYEGLAGLTDSLRESRPAGLKVFGDWPTIYPGIQKLPSRAKESWF
                     EFDHHYSDEAYPKALPLVFTDGIDSITDVGGNTGKWARSCCAWRSDIRVAVIDLPEQC
                     ETVDRVMAEEGLASRVTTHPMNVLSDQPFPKTGTKAWWMSQFLDCFSPSQIVSILKKA
                     HAAMEDDAVLYILEPLIGRQPFPVADACLAAMSLYFTALANGTSRFYPYDEFEVFVEA
                     AGFEIERVHDGLGMEHTLLVCRKKKA"
     gene            4657..5388
                     /locus_tag="ACZPB6_05170"
     CDS             4657..5388
                     /locus_tag="ACZPB6_05170"
                     /inference="COORDINATES: protein motif:HMM:NF025100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HIR1183897.1"
                     /translation="MMTAFDIASHVVLAQGLSSGEDLRAWAQAALPGMPEGLPDAPLA
                     FTKRLPMMKARRMSPGARLACEAALALEGAGEAKAWIFSSRHGETVRGVKILDAISAG
                     EPPSPTDFMMSVHNAAAGMFTIEGRVHAPASSVAAGAESFHMALVEAAGMLVDGMDCV
                     AVVDFDDCQPGALLDAFESRPCDALYATAWLLKKGAGVTMTPRSLKQAEPVLPASLQL
                     AAGLASERSAFVTSGAATRFEWERA"
     gene            5400..6239
                     /locus_tag="ACZPB6_05175"
     CDS             5400..6239
                     /locus_tag="ACZPB6_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1183898.1"
                     /translation="MTQEKAERTEGRLAFEWRRLKTGLCWVIFGLGGLGLSMTYFPYL
                     RLTVRDPVARRLKARAMIARCFDFYARTMDRLGVMMIERGSLAQMRSLRGTIIVANHP
                     TILDYVLTASMLPEMDCLVKASLRHNFFLKHVIRAADYMSNDGSLETLEEITRRLEAG
                     ENILIFPEGTRTRAGVSMKLKRGVAHAALRTGCRLAVVHIRSSHAWLNKDTQWYEIPD
                     VRPSIRLTFEGMIDPKDYLREGEDGYSLASRRLTEAMRRELLKAAGREDEIEGKARDR
                     RQN"
     gene            6302..6568
                     /locus_tag="ACZPB6_05180"
     CDS             6302..6568
                     /locus_tag="ACZPB6_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HIR1183899.1"
                     /translation="MNNLENEVKALIIEALNLEDMTPEDIETDAPLFGEGLGLDSIDA
                     LELGLAVKKRFGVSLSGETEEVRAHFRSVASLAAFICENRTEEK"
     gene            6572..6847
                     /locus_tag="ACZPB6_05185"
     CDS             6572..6847
                     /locus_tag="ACZPB6_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1183900.1"
                     /translation="MAKTYTKEELFDEIVRVMGDLFEVDTEGVTRESRLVEDLDLDSI
                     DAVDMIVHLQNMIGKRIKPEAFKSVRTVGDVVDVIYDFSQQNGEAAK"
     gene            6860..7426
                     /locus_tag="ACZPB6_05190"
     CDS             6860..7426
                     /locus_tag="ACZPB6_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG4648 family protein"
                     /protein_id="HIR1183901.1"
                     /translation="MTGTILNAVTAAVMAVWPFFVLYAVTNGLMTLLLAGTACVLGVR
                     LALAFAGRGETARSGRLLSAAGLVLVGLAALLDEADWMLWYPVLVNASLLSVFGASLF
                     SGMPVVERLARLGLRGKPLPPEGVAYTRRVTAVWCGFFVVNGSIAVATILADDLTLWA
                     LWNGGLSYVAIGCLMGGEYLYRKLVKHV"
     gene            7419..>7841
                     /locus_tag="ACZPB6_05195"
     CDS             7419..>7841
                     /locus_tag="ACZPB6_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183902.1"
                     /translation="MSEAERSPVAGWLEGRPEAPAAVHAGGAVTTRAELARRVSAAAA
                     ALAGMPAGSRIAVDADAPHDFAVLLSAVLWRGCVPVLFAGRGAMHETLADEYDAVLTT
                     ESHRSFPKPALVADFAENPCPAPELRAPEAERPLVLFTS"
BASE COUNT         1307 a         2543 c         2615 g         1376 t
ORIGIN      
        1 gcaaagagcg gctggtcctc aagaagcttc ctgaggcgct cggcctcgag ctcccaggcg
       61 tcgggatcga cgagcgttgc ggcggcaagg cgtcttgcgg tcgcgcaggc aggtgcggcc
      121 gcatagagct tctggctctc ggtcgcctgc cattcgcgaa gcgcatgaag ctcggcctcg
      181 cggtgacggg ccgtgtgcac gggcgcgagc acgtcggtcg caaacctgcg ggccgcctcg
      241 agcggatcgg cggattcaag gagctccttc atgccctcgg cttcgatgcc ggcggaaaga
      301 agccctttaa tatagggaac gaagaccctg cgccaccaga tgcaggtgtc ggatagcgac
      361 gtgccgtagc gcgtgaagag cgtgcgcacg gcgttgttgc cgtaagtggc gaaggcgggt
      421 gcgtccgcgc gttcggcaag gctgtcccag gtgcgggcga cggtcttcac ggcggccttg
      481 aggcgcgcgt gcagcgcgac ggcttcgaag gcctccttcg aggcagggcg cgagccgtcc
      541 acctcgacgc cggcaagcgc atcggcggcc tcgcgggcct ccttgttgaa gaggcggccg
      601 aggaagcccg tgctgccgtc gggatcgttg gctgtgaacc attcggcggc cttcatggcg
      661 gcgtcggcgt cgatggcgtc ggggatgacg acggtgcggt tgtcaagaag cgaggcgcgg
      721 tcgacggcga gagcaagttc gtttgcggcc gcgagctcgc gctcgagctg ctcgaaggcc
      781 tcgcggcacc cttcgtccgc aaggccgagc gagagcgcaa ggcgcttcat ttcgtccgtt
      841 tcggtaagcg gcgaaagatc catagcgggc gtgagcgcgg agatgcccga ctcgggtgcg
      901 acgagaagcg tttcgccgcc cttcatgaag gcgtggagcc gttcgcctga ggcgctcctt
      961 cgctcgtcga tggtgacgtc gcctgcgtcc tcggcgagca cgtcgcgcca acggcggttc
     1021 atgccgcgca tcgtgtcgac gtcgggcaga agctcgaatc cggggagcag gcccgagagg
     1081 tcgcgggccg tctcggcgtc ccaccggccg ttcgtgccgt atagttcaag aagctccctg
     1141 aaggagagcg gcaggggacc ttcggccact tcgcccggaa tgagcgcctg caggcgctcg
     1201 gcctcgcgca ggaacttcgc gatttcggtg agcgtgtagc ggcggccgtt gaattcgacg
     1261 gcctcgtgct cgatcctgcg ctgctcgaag atgcggcggc gacggtcggc gagcgcggtg
     1321 ccgagtcgat gacgttcaag gccgagcgcc ctgatgcggg cctcgaggtc ctcgacgttg
     1381 aggcgggtga ggcgcgtgat gagttcggac gaggtctgct cgaggtcctt cctgtcgccg
     1441 agcatcgtga cgcagagcgg ctggatctcc ttcggcagga ggtccttgac gactgagagc
     1501 gccttcgtcg tgtggctcgt gacgagcacg cgcttgccct gcgagaggaa gtggccgaga
     1561 aggttcgcga tggtgtgggt cttgcctgtg ccgggagggc cctgcacgag cacgacgttg
     1621 ttgtgttcga cctcgtgcgc gatcgcgagc tgctcggggt tcgcgggctt ggcgagaagt
     1681 acgtcggggt cctcgcccgc cgtcgcggcg agtttggctt cgagcgaaag cgtgggcgtg
     1741 aggcccgagg cgagcgtcgg gaagacgtcg ggacagacga tctccatcag gtgcgcgggc
     1801 acgtcgcggt gttcggcgat gtccttcctg atgctgcgga tcgcactgcg aacgccgctc
     1861 ggacgcggct gaataagaat gacgggactc gcctcgagcg tgaaggcggg tttttcaccc
     1921 gcgtcctcgg ggagttcttc ttctgcgggc atgaagcggc agtctgggtg aaggcttgcg
     1981 gcgaactttt cgagcgcctc ggaaagcgac tcgtcgcccg caaggttcgc gtccgcaccc
     2041 gcatcctcga gccattcctc aaagcgcgag gcggccttga ggtcgatgcc gtcctcggca
     2101 aagggcgcga ggatttcggc aaggagcctc gggtcctcgt cgccgtttct gcgcacttcg
     2161 atcacgggca ggttgcggcg gctcgagccg agctcgatga cgagcggctg cacgaggagc
     2221 ggccagcgca cggggttctt cgcgcgcgtg acgtcggggt tcgacgtgaa gacggccgtg
     2281 ccgatcacgc actcgtcgcg aaggttcgac tcggagagat ggtccctcag atcctgcagg
     2341 cgctcgaaga gcgcatggtt gggaagaatc tcgcctctgg cctccagcca ggagccgcgg
     2401 atcctgagcc agtcggcgag cgtctcgtag agttcggccg actcgggatg ctcggccgga
     2461 tcgggcaggg ccttccagtc gggcgtccac tcgggggcgc gccagtcgcc cgtcgcatac
     2521 ggggcgagag actcgggaat cggcgggcac ggcggcattt cgagcttttc gatgcggcag
     2581 acgaggtcgg cgtcggcgcc cgtcgggtcg cccgcaacga gccccggcac ggccgcctcg
     2641 agctcgggga gcctgagaag cctgccgcac ctcgagaggt gccttgcggc cgtctgcctg
     2701 aggctcgtgg cctcttcaat gaaggtgaga agactgtcga ttgcttcgcg ctgtgcttcg
     2761 gtggctatca tcggaattgt cttggctgcc gtccggagaa tcgcccggcc ggaaacggat
     2821 ttcggaatct aaacactaca ttgtactgaa gcggacgcaa tccgcctcca catggggcgt
     2881 cggacagcgt ccgcagcttc gcaaatgcgt cgctgcaggc gtgcggtctt gtgaagtgat
     2941 gatatcggga ggcttgagga aagaggagga atctgcggaa atcccgtgcg agcgaacgga
     3001 atcaggtaga atcgtgatat ttcctgaaga cgcaagaact gaatgaatgt tcattcagga
     3061 gcacattccg ggaggtccct gcgccgccga tccctgcagg acggggagag cagggctcgt
     3121 gtcgcaacaa aacaacttca aaaggagagg ccaacatgct tcgccgcaat cttcttctcg
     3181 gcgtcgccgg cgtcgccgtt ctcggcgtca cgggctgcgc aacccatccc gtcagcgtcc
     3241 agcaggccgc cgagcttccc gccgcccagc ttcgcacgtg catcaagcgc gctgcccatc
     3301 gtcgcggctg gcgcgtcgtc acggatgaac cgggcaagat gcgcatcaag tacgtcaagg
     3361 gtccccatgt cctcgtcgcc gacgtcacct atgacaacga gggctacatg atcgaagccg
     3421 tgaaggaagg ctcgacgctc ttcaacaagg acggcacggc tcaccgcaag gtcaattcct
     3481 ggacggccaa cctcgccgtg acgatccgtg aggaagaaaa caagatcctc cttcgccaga
     3541 cgaactgatt gtcaacgatc cgaccgccga gccgatgaaa gagtccatca aaaagacccg
     3601 ccgcacgccg gcccttgaag ccctcaacga ggcccatcgt cttgccgtct ctcccttcgt
     3661 cttccaggcg ctctgcgcca tgacggacct cgggatgatg aaggcgctcg acgaggccgg
     3721 ccccgaaggc ctcaccctcg aggcgctctg tgaaaagtcg ccctgcagcg actacgccgc
     3781 cagggcgctc cttgaggtcg ccgtgcgatt cggcgtggcg cagcggtcgg atgaaggccg
     3841 cttctcgctc acgaacacgg gcatcttctt tgtcaacgac ctgcacgtgg gcatcaactt
     3901 ccgcttcacg cgcgacgtct gctacgaggg cctcgcgggc ctcaccgact ccttaaggga
     3961 gtcccgtccc gcaggcctca aggtcttcgg cgactggccg acgatctatc ccggtatcca
     4021 gaagctgccg agccgtgcga aggagagctg gttcgaattc gaccaccatt attccgacga
     4081 ggcctatccc aaggcgcttc cgctcgtctt caccgacggg atcgactcga tcacggacgt
     4141 cggcggcaac accggcaagt gggcccgctc ctgctgcgcc tggcgcagtg acatccgcgt
     4201 ggccgtcatc gacctgcccg agcagtgcga gaccgtcgac cgcgtgatgg ccgaagaagg
     4261 cctcgcctcc cgcgtcacca cgcatccgat gaacgtcctc tccgaccagc cgttcccgaa
     4321 gacgggcacg aaggcctggt ggatgagcca gttcctcgac tgcttctccc cgtcgcagat
     4381 cgtctccatt ctgaagaagg cgcatgccgc gatggaggac gatgcggtcc tctatattct
     4441 tgagccgctc atcggccgtc agccgttccc cgtggccgac gcctgccttg cggcgatgtc
     4501 gctctacttc acggcgctcg ccaacggcac gagccgcttc tatccctacg acgagttcga
     4561 ggtgttcgtc gaggccgcgg gcttcgagat cgaacgcgtc cacgacggcc tcggcatgga
     4621 gcacacgctc ctcgtctgcc gcaagaagaa ggcctgatga tgacggcctt cgacatcgcc
     4681 tcccacgtgg tgctcgcgca ggggctctcc tccggcgagg acctccgcgc atgggcgcag
     4741 gctgcgctcc ccggcatgcc cgaggggctt cccgacgcgc cgctcgcctt cacgaagcgg
     4801 cttccgatga tgaaggcgcg ccgcatgagt ccgggcgcaa ggcttgcctg cgaggcagcc
     4861 cttgcacttg agggcgcggg cgaggcgaag gcctggatct tctcgagccg ccacggcgag
     4921 accgtgaggg gcgtgaagat cctcgacgcg atttcggcgg gcgagccgcc gtcgcccacg
     4981 gacttcatga tgtcggtcca caacgccgcc gcaggcatgt tcacgatcga gggacgggtg
     5041 cacgcgcccg cctcgagcgt cgccgcaggc gccgaaagct ttcacatggc gctcgtcgag
     5101 gcggcgggca tgctcgtaga cggcatggac tgcgtggccg tcgttgactt cgacgactgt
     5161 cagcccgggg cgcttcttga tgcgttcgag tcgcgcccct gcgatgcgct ctacgccacc
     5221 gcctggctcc tgaagaaagg cgcgggcgtc acgatgacgc cgcgttccct gaagcaggcc
     5281 gagcccgtgc ttcccgcttc tctccagctc gccgcaggcc ttgcgtctga aaggtccgcg
     5341 ttcgtcacgt cgggcgccgc cacccgcttc gagtgggagc gtgcgtgatg gaggccgccg
     5401 tgacgcagga aaaggccgaa cgcaccgaag gcaggctcgc cttcgagtgg cgccgtctca
     5461 agacggggct ctgctgggtg atcttcggac tcgggggcct gggcctctcg atgacgtact
     5521 ttccctatct gaggctcacg gtccgagatc ccgtggcgcg ccgcctcaag gcccgcgcga
     5581 tgattgcgag gtgtttcgac ttctatgcgc ggacgatgga tcgccttggt gtcatgatga
     5641 ttgagcgcgg gtcgctcgca cagatgcgct ccctcagggg aacgatcatc gtcgccaacc
     5701 atccgacgat tctcgactac gtcctcacgg cctcgatgct ccccgagatg gactgtctcg
     5761 tgaaggcgtc gctcaggcat aatttcttcc tcaagcatgt gatccgtgcg gccgactaca
     5821 tgtcaaacga cggctctctg gaaacgcttg aggagatcac ccgcaggctt gaggccggcg
     5881 agaacatcct gatcttcccc gagggcacgc gcacgcgcgc gggtgtctcg atgaagttga
     5941 agcgcggcgt cgcccatgcg gccctgcgca cgggatgcag gctcgccgtc gtgcacatca
     6001 ggagctccca cgcgtggctc aacaaggaca cgcagtggta cgagattccc gacgttcgcc
     6061 cgtcgatccg cctgaccttc gagggcatga tcgatccgaa ggactacctg agggaaggcg
     6121 aggacggcta ctcactggcg agccgcagac tcaccgaggc gatgcgccgc gaactcctca
     6181 aggccgcggg ccgcgaggac gagatcgaag gaaaggcgcg cgaccgccgg cagaattgac
     6241 gcgctgcgca gaaggcggca gcgcttgcga accaatccct catacaacgg atgaagaact
     6301 gatgaacaat ctggaaaacg aagtcaaggc cctcatcatc gaggccctca accttgagga
     6361 catgacccct gaagacatcg agacggacgc tccgctcttt ggcgaaggtc ttggtctgga
     6421 ctccatcgac gccctcgagc tcggccttgc cgtcaagaag cgcttcggcg tctcgctctc
     6481 gggcgaaacg gaggaggtgc gcgcacactt ccgcagcgtc gcctcgctgg ccgccttcat
     6541 ctgcgaaaac cgcaccgagg agaagtaaga aatggcgaag acctacacca aggaagaact
     6601 gtttgacgaa atcgtccgcg tgatgggcga tctcttcgag gtcgacaccg aaggcgtcac
     6661 ccgcgaaagc cgcctcgtcg aggacctcga ccttgacagc atcgacgccg tcgacatgat
     6721 cgtgcacctg cagaacatga tcggcaagcg catcaagccc gaggccttca agtccgtgcg
     6781 cacggtgggc gacgtcgtcg acgtcatcta cgacttctcg cagcagaacg gcgaagccgc
     6841 caagtaagcg gcgcgcctga tgacgggcac gattctcaat gcggtcacgg ccgccgtcat
     6901 ggcggtctgg ccgtttttcg tcctttacgc cgtgacgaac ggcctgatga cgctgctgct
     6961 ggcgggcact gcctgcgtgc tcggcgtgag gctcgcgctc gcctttgcgg ggcgcggcga
     7021 gaccgcccgc agcggccgcc ttctttcggc tgcgggcctc gtgctcgtgg gccttgcggc
     7081 ccttcttgac gaggccgact ggatgctctg gtatcccgtc ctcgtcaacg cctcgctcct
     7141 ctccgtcttc ggcgcctcgc tcttttcggg catgcccgtc gtcgagcgcc tggctcgcct
     7201 cggcctgcgc ggcaagcccc tgccgcccga aggcgtcgcc tacacgcgcc gcgtgacggc
     7261 cgtctggtgc ggcttcttcg tcgtcaacgg cagcatcgcc gttgcaacga tcctcgcgga
     7321 cgacctgacg ctctgggccc tctggaacgg ggggctgagc tacgtcgcca tcggctgcct
     7381 catgggcggc gaatatctct acaggaagct tgtcaagcat gtctgaagcc gagcgctctc
     7441 ccgtcgcagg ctggctcgaa ggccgccctg aggcgcccgc cgcagtccat gcgggcggcg
     7501 ccgtgacgac ccgcgccgaa ctggcgcgca gggtctcggc cgctgccgcc gcactcgcag
     7561 gcatgcccgc gggctcgcgc atcgccgttg acgccgatgc gccgcacgac ttcgcggtgc
     7621 tcctctcggc cgtcctctgg aggggctgcg tgcccgtgct ctttgcgggc cgcggtgcca
     7681 tgcacgagac gctcgcagac gaatacgacg ccgtcctcac gacggagtcg cataggtcgt
     7741 tcccgaagcc cgcgctcgtc gcggacttcg ccgagaatcc gtgcccggct cccgaactca
     7801 gggcgcccga ggcggaaagg ccgctcgtac tcttcacgtc g
//
LOCUS       DBMDTI010000079         7830 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2503_length_7830_cov_2.899081, whole genome shotgun sequence.
ACCESSION   DBMDTI010000079 DBMDTI010000000
VERSION     DBMDTI010000079.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2503_length_7830_cov_2.899081"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="ACZPB6_05200"
     CDS             <1..914
                     /locus_tag="ACZPB6_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1183903.1"
                     /translation="IRRIASFAEKMVPIMALIYVIGSLTIIFTNLEGVMHALEMIFVG
                     AFDPMAATGGVIGAGIKEAIRYGVARGLFSNEAGMGSTPHAHAVARVKHPAEQGLAAI
                     IGLFIDTFIVVNMTAFVILCTGSLDGTTTGIALTQKAFTLGLGTIGNGFVAVCLLFFA
                     FTTIVGWYFFAEQNVKYLVGVRYVSIYRVLVLAFIMAGSFLHVTLVWELADLFNGLMA
                     IPNIIALIGLSKIVGRALEDYEKKFLAGETPEFGELAPVPADIPRYNGAPAQDPALEP
                     KPQHHRRLGGLRNRFRRRPHNAQENDA"
     gene            complement(1010..1210)
                     /locus_tag="ACZPB6_05205"
     CDS             complement(1010..1210)
                     /locus_tag="ACZPB6_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HIR1183904.1"
                     /translation="MKKIMLAAMMICGAVALAGCNTVAGFGTDLKKGANHVGTALESI
                     GSKGQSSQAPAKSAESKPVEDK"
     gene            complement(1252..2715)
                     /locus_tag="ACZPB6_05210"
     CDS             complement(1252..2715)
                     /locus_tag="ACZPB6_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR1183905.1"
                     /translation="MTTDTLLLILTGLCAFLAAAVVWLVLEMRLLKIQTSENLRRDEQ
                     DEMIEAILTGVRSERQEVQQAVLNVERGVSETLAGLTGYLTEQQNRSAAEVRTLNETA
                     RQSLAETREVMNRQLLELQGLVDARFAKIIETNASASEALGAKLESKFSEIRTSVDAS
                     LEKVRAGNEARLEEMRATVQEKLDRTLSERLTASFRTVDEKLGLVQTGLGEMRTMAES
                     VRDLKGILANVKTRGTFGETQLAAILANILTPAQYGAQVRVVPGSREAVDFAVRMPGA
                     SGDGPCWLPMDSKFPVEDYARLLDAESRADAAAAAQARVALERAVLVQAKSIHDKYVR
                     PPYTTEFAVMYLPSEGLYAEVIRIPGLFEKLQRDWRITPAGPTVVSALVNSLQMGFVT
                     LALQERSSEVWKVLGEVKGEFLQFAEGFAKVQKKFSEAQSSLDAMKTRQNVMQKKMSS
                     IEAAGLLEEDGQEETGAADKLSQGVRGSNASVAAGLN"
     gene            complement(2879..3976)
                     /locus_tag="ACZPB6_05215"
     CDS             complement(2879..3976)
                     /locus_tag="ACZPB6_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1183906.1"
                     /translation="MQKRTAFKLCLAGATAVALNAAPVAVFAKDEPLKVAFVYVSPAN
                     EEGWSSQHDIARKFVEEKFGDKIKVTWIENIPENADAQRVIRDLAQQGNKIIFATSFG
                     YMNSVMKVAKQFPNVYFEHATGYKTSKNVKNYTARFYEGRYLAGMLAAATTKTNILGY
                     VAALPIPEVFQGINAYTLGARAVNPNIEVRVVWTSSWYDPGKEADATKALIGMKADVI
                     THHTNSTAVASTCEEAKVPVISYNSAMHKAAPTMLLGGVVHRWEEYYTNEIQKVLDGK
                     WDNTPVWGGANVHMIELADMTPKAPENVASRIKATYAKLEKGEFHPFTGPVVSNEGKV
                     MIPEGKVATDEELQQMMYFVEGVASKVPTAK"
     gene            complement(4069..4989)
                     /locus_tag="ACZPB6_05220"
     CDS             complement(4069..4989)
                     /locus_tag="ACZPB6_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1183907.1"
                     /translation="MSLAASIISSTLNAGTPLLIAAVGIIIHEKSGVLNLGIEGIMLM
                     GAVSGFAVTHATGSFELGFLAGMGVGLLLGLLFAFFTIGLRANQYAAGLALTLFGTGL
                     SAFLGQSLQGAALPGRAPLGIPGLEGIPFLGEAFFSQHWLAYAALAMIAGVWYFLFKS
                     RPGLILRAVGESPESAFALGYPVRLIRFWAVLFGAMMAGLAGAYLSLVYTPLWVEGMV
                     AGRGWIALALVTFATWRPARVLVGAYLFGGVTMLQFAVQGMGIDIAPQFMAMTPYIAT
                     ILVLVLISRNPAWIRLNVPASLGKPFAPRS"
     gene            complement(4989..6071)
                     /locus_tag="ACZPB6_05225"
     CDS             complement(4989..6071)
                     /locus_tag="ACZPB6_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1183908.1"
                     /translation="MNFPIAMTSRQEPARSLRWLSPALALLLTVVGGAVIFAALGKDP
                     VEALVTFFISPLDSLSGWAEVCVKMTPLLLCSVGLVVCFRANVWNIGAEGQLVAGAIA
                     GGAVALMCNDTTGPWFVVLVMLASALGGALWGAITALLRHKFHANEILVSLMLVYVAQ
                     FLLSWAVQGPLRDPDGFGFPQTALFESGALLPVILEGTRLHAGALMAVIAALGIWVLM
                     DKMEIGMEFKISGMAPLAARYAGYRPGHLIWISMLICGALAGLAGGIEAAGPLGQLTP
                     TISPGYGFAAIIVAFVGRLTPLGCVPAAFIMALFYLGGELAQSRLGLPSSITGVYQGL
                     LLFFILACDTMIFYQFKWIGFKKEAA"
     gene            complement(6068..7594)
                     /locus_tag="ACZPB6_05230"
     CDS             complement(6068..7594)
                     /locus_tag="ACZPB6_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1183909.1"
                     /translation="MHPRLSLKNITKRYPGIVANDGINMDIAPGEVLAVLGENGAGKS
                     TLMKIIYGSVRPDEGSLEFDGRPLDVSSPAEARASGIAMVHQHFALFDTLTVAENVAL
                     GLPPGRSTAEISEEVKTLGEKYGLEVDPAAVVYELSMGERQRVEIIRALMTNPKLLIL
                     DEPTSVLVPQAVRKLFVTLHRLAEEGVSILFISHKLDEIRELADRCVVLRAGRIVSSV
                     NPKAETEASLARLMIGAEPPAIREASGTPGEVVFEMKDVSYPGSLRVCGIENVSVAVR
                     KGEIVGVAGISGNGQARLMAVAAGEAPVADEGVMLFGKPVGSLGTRDRRRLGLRYVPE
                     QRLGHAAVPEISLQTNTYLTGDDLVKHGFILEEKALRFTEKIIERFHVKTPSPQKHAG
                     GLSGGNLQKFIMGREILQNPGVLIVNQPTWGVDVGAAATIRNALMRLREDGAAVLVVS
                     EEVDELFEICDRIAVMYRGHLSPAVPKSEMNIEIVGRWMSGLWPDGPAGASGHEGGEA
                     "
BASE COUNT         1459 a         2516 c         2507 g         1348 t
ORIGIN      
        1 gcatccgtcg catcgcctcg ttcgccgaaa agatggtccc gatcatggcc ctcatctacg
       61 tcatcggctc gctcaccatc atcttcacga acctcgaagg cgtgatgcat gcccttgaaa
      121 tgatcttcgt gggcgccttc gacccgatgg ccgccaccgg cggcgtcatc ggcgccggca
      181 tcaaggaagc catccgctac ggcgtcgccc gcggcctctt ctcgaacgaa gccggcatgg
      241 gctcgacccc gcacgcccac gccgtggccc gcgtcaagca ccccgccgaa caggggctcg
      301 ccgccatcat cggcctcttc atcgacacgt tcatcgtcgt gaacatgacc gccttcgtaa
      361 tcctctgcac gggttcgctt gacggcacga cgaccggcat cgcgctcacc cagaaggcct
      421 tcacgctcgg tctgggcacg atcggcaacg gcttcgtcgc ggtctgcctg ctcttcttcg
      481 ccttcacgac catcgtcggc tggtacttct tcgccgagca gaacgtcaag tacctcgtcg
      541 gcgtgcgcta cgtctcgatc taccgcgtgc tcgtcctcgc cttcatcatg gccggctcct
      601 tcctccacgt caccctcgtg tgggagctcg ccgacctttt caacggcctg atggcgatcc
      661 cgaacatcat cgccctgatc ggtctgtcga agatcgtggg ccgcgcgctc gaagactacg
      721 agaagaagtt cctcgcaggc gaaacgcccg aattcggcga actcgccccg gtccccgccg
      781 acatcccgcg ctacaacggc gccccggccc aggaccccgc gctcgaaccc aagccgcagc
      841 atcaccgtcg ccttggcggc ctgcgcaacc gcttccgccg tcgtccgcac aatgcgcagg
      901 aaaacgacgc gtaagcacgg ccctcaccca gacctgaacc ccgcgcttag aaaaaggacc
      961 cgcttcgacg ttcgaagcgg gtcccgttca ttcgcttgac ggaaaggact tacttgtcct
     1021 cgaccggttt ggattctgcc gacttggcag gcgcctgact gctctggccc ttggaaccga
     1081 tgctctcaag cgccgtgccg acgtggttgg cacccttctt gaggtcggtg ccgaaaccgg
     1141 cgacagtgtt gcatccggca agcgcaacgg cgccgcagat catcatggcg gcaagcatga
     1201 tcttcttcat atttactcct tgaaagcgcc gcacccgcgg cggccatgcg ttcagtttag
     1261 cccagccgca acgctcgcgt tcgaacctcg tacgccttga gacagtttgt ccgcggcgcc
     1321 cgtctcttcc tgcccatctt cctcgagaag accagccgcc tcaatcgaac tcatcttctt
     1381 ctgcatcacg ttctgccgcg tcttcatcgc atcgaggctc gactgcgcct cggagaactt
     1441 cttctgcacc ttcgcgaagc cctcggcaaa ctgcaggaac tcacccttga cctcgccgag
     1501 caccttccag acctcgctcg aacgctcctg aagcgcaaga gtcacaaagc ccatctgcag
     1561 gctgttgacg agcgccgata cgaccgtcgg ccccgcgggc gtgatccgcc agtcgcgctg
     1621 aagcttctcg aagagccccg gaatcctgat cacctccgca taaagtccct cgctcggcag
     1681 gtacatcacg gcgaactccg tcgtgtaggg cggcctgacg tacttgtcat gaatcgactt
     1741 tgcctgcaca aggacggccc tctcaagcgc caccctcgct tgggcggccg cagccgcatc
     1801 cgccctcgac tcggcatcaa gcaggcgtgc atagtcctcg acagggaact tcgagtccat
     1861 agggagccaa caggggccgt ctcccgaagc gcccggcatc ctcaccgcaa agtccaccgc
     1921 ctcccggctg cccggcacga ccctcacctg ggcgccgtac tgcgctggcg tgaggatgtt
     1981 ggcgaggatg gcggcgagct gcgtctcgcc gaaggtgccg cgggtcttca cgtttgcgag
     2041 gatgcccttg aggtccctga cgctctcggc catcgtacgc atctcgccca gccccgtctg
     2101 cacgaggccg agcttctcgt ccacggtcct gaagctcgcc gtaaggcgct cggagagcgt
     2161 gcgatcgagc ttttcctgaa ccgtcgcacg catctcctcg aggcgggcct cgttgcctgc
     2221 gcgcaccttc tcaaggctgg cgtccacgct cgtgcggatc tcggagaact tggactcgag
     2281 cttggcgccg agcgcttcgc tcgccgatgc attcgtctcg atgatcttcg cgaagcgcgc
     2341 gtccaccagc ccctgcagct caaggagctg gcggttcatc acctcgcgcg tctcggcaag
     2401 cgactgtctg gccgtctcgt tgagcgtacg cacctcggcc gcgctcctgt tctgctgctc
     2461 ggtgaggtag ccggtcagcc ccgccagcgt ctccgagacg ccgcgctcga cgttgaggac
     2521 cgcctgttgc acctcctggc gctcgctcct cacccccgtg aggatcgcct caatcatctc
     2581 gtcctgctcg tcgcgccgca ggttctccga agtctggatc ttcagaaggc gcatttcgag
     2641 cacgagccag accacagcgg cggcgagaaa ggcgcacagg cccgtaagta tcagaagaag
     2701 cgtgtcggtc gtcatgtcgg catccggaaa aaatgcaggg gcgctcgccc cgttcggaac
     2761 gctatcttag ccgagcctgc gaaaagactc atgcctcgca cgacgcgtgc cgagaatgca
     2821 aaaaggcctc cgcctcccga aggaggaaga ggccctgaat caagcccttg agagcggatt
     2881 acttggcggt cgggaccttg ctggccacgc cttcaacgaa gtacatcatc tgctggagtt
     2941 cttcgtcggt ggcgaccttg ccttccggaa tcatgacctt gccttcattg ctgacaaccg
     3001 gacccgtgaa cggatggaac tcgccctttt caagcttggc gtaggtggcc ttgatgcggg
     3061 aggcgacgtt ttccggagcc ttcggggtca tgtcggcaag ctcaatcatg tggacgttcg
     3121 cgccgcccca aaccggggtg ttgtcccact tgccgtcaag caccttctgg atttcgttcg
     3181 tgtagtactc ctcccagcgg tgcacgacgc cgccgagaag catcgtcgga gcagccttgt
     3241 gcatggcgga gttgtaggag atgaccggaa ccttggcttc ttcgcaggtg gaggcgacgg
     3301 ccgtggagtt cgtgtgatgc gtgatcacgt cggccttcat gccgatgagg gccttcgtgg
     3361 cgtcggcttc cttgcccgga tcgtaccagg aggacgtcca gacgacgcgg acctcgatgt
     3421 tcgggttcac ggcgcgggcg ccgagcgtgt aggcgttgat gccttggaac acttcgggaa
     3481 tcggaagggc ggcgacgtag ccgaggatgt tggtcttcgt cgtggcggcg gcgagcatgc
     3541 cggcgaggta gcggccttca tagaagcggg ccgtgtagtt cttgacgttc ttgctcgtct
     3601 tgtagcccgt ggcgtgctcg aagtagacgt tcgggaactg cttggcgacc ttcatgacgg
     3661 agttcatgta gccgaacgac gtcgcgaaga tgatcttgtt gccctgctgg gcgaggtcgc
     3721 ggatgacgcg ctgggcgtcg gcattttcag ggatgttctc gatccaggtc accttgatct
     3781 tgtcgccgaa cttctcttcg acgaacttgc gggcgatgtc atgctgggag gaccagcctt
     3841 cctcgttggc cgggctgacg tagacgaagg cgaccttcag cggctcgtcc ttggcgaaga
     3901 cggcgaccgg ggcggcgttg agcgcgacgg cggtggcgcc ggcgaggcag agcttgaagg
     3961 cagtacgttt ttgcatggat gaattctcct gaagacagaa gtaaaagaat caatggctag
     4021 tttagccaag aaggcaggac atgcctacct tctcgccagg tgaaattctt agctgcgcgg
     4081 cgcgaacggc ttgccgagcg aggcgggcac gttgaggcgg atccaggcgg gattgcggct
     4141 gatgagaacc agcacgagaa tcgtcgcgat gtacggcgtc atggccatga actgcggcgc
     4201 gatatcgatg cccatgccct gaaccgcgaa ctgaagcatc gtgacgccgc cgaagaggta
     4261 ggcgccgacg agcacgcgcg cgggacgcca ggtggcgaac gtgacgagcg caagtgcgat
     4321 ccagcctcgg cccgcaacca tgccctcgac ccagagcggc gtgtagacga gcgagaggta
     4381 ggcgcccgca agccccgcca tcatggcacc gaagagcacg gcccagaagc ggatgaggcg
     4441 cacgggatag ccgagcgcga atgcagattc gggagactcg ccgacggctc ggagaatgag
     4501 gccgggacga gacttgaaga ggaagtacca gacgcccgcg atcatcgcaa gcgcggcata
     4561 ggcgagccag tgctgggaga agaacgcctc gccgaggaac ggaatgccct cgaggcccgg
     4621 aatgccgagc ggagcgcggc cgggaagcgc ggcgccctgc agggactggc cgaggaaggc
     4681 cgagagcccc gtgccgaaga gcgtgagcgc aagacctgcg gcgtactggt tggcgcgaag
     4741 gccgatcgtg aagaacgcaa agagcaggcc gagcagaagg cctacgccca tgcccgcgag
     4801 aaagccgagc tcgaagctgc ccgtggcatg cgtcaccgca aagcccgaaa cggcgcccat
     4861 gagcatgatg ccctcaatgc cgaggttgag cacgcccgac ttctcatgaa tgatgatgcc
     4921 gacggcggcg atgagaaggg gcgtccccgc gttgagcgtg gaggaaatga tcgatgcggc
     4981 gagactcatc aggcagcctc cttcttgaag ccgatccact tgaactgata gaagatcatc
     5041 gtatcgcagg cgagtataaa gaagagaaga aggccctggt agacgcccgt gatcgaggac
     5101 ggaaggccga ggcggctctg cgcgagctcg ccgcctagat agaagagcgc catgatgaac
     5161 gcggcgggca cgcagcccag cggcgtcagg cgccccacga aggcgacgat gatagctgcg
     5221 aagccatacc ccggagagat ggtcggggtg agctggccga gcggacccgc ggcctcgatg
     5281 ccgccggcaa gccccgcgag ggcgccgcag atgagcatcg agatccagat caggtgaccc
     5341 ggacggtagc ccgcataacg ggcggcgagc ggcgccatgc cggagatctt gaactccatg
     5401 ccgatctcca tcttgtccat gaggacccag atgccgagcg cggcgatgac ggccatcagg
     5461 gcgccggcgt gcaggcgggt gccctcgagg atgacgggca ggagcgcgcc gctttcaaag
     5521 agcgcggtct gcgggaagcc gaagccgtcg ggatcgcgaa gcggcccttg gacggcccag
     5581 gagagcagga actgcgccac gtagacgagc atgagcgaga caagaatctc gttggcgtgg
     5641 aacttgtggc gaagaagcgc cgtgatcgcg ccccagagag cgccgccgag cgcagaggcc
     5701 agcatgacga ggacgacgaa ccaggggccc gtcgtgtcgt tgcacatcag ggcgaccgcg
     5761 ccgcccgcaa tggcgcccgc aacgagctgg ccctcggcgc cgatgttcca gacgttggcg
     5821 cggaagcaca ccacgaggcc cacggagcag agcagcaacg gcgtcatctt gacgcacacc
     5881 tcggcccagc cgctcaggct gtcgagcgga gagatgaaga aggtgacgag ggcctcgacg
     5941 ggatccttgc cgagcgcggc gaagatcacg gcgcccccga cgaccgtgag caggagtgca
     6001 agcgcgggcg acagccagcg caggctgcgg gcgggctcct gacgcgaggt catggcaatg
     6061 gggaaattca tgcttcgcct ccttcatgac cggacgcgcc ggcggggccg tcgggccaga
     6121 gaccggacat ccagcggccg acgatttcaa tgttcatttc agacttggga acggcgggag
     6181 agagatggcc gcggtacatg accgcgatgc ggtcgcagat ctcgaagagt tcgtcgactt
     6241 cctcggaaac gacgagcacg gccgcgccgt cctcgcgcaa gcgcatcagc gcgttgcgga
     6301 tcgtggcggc ggcgccgacg tcgacgcccc aggtgggctg attgacgatg agcacgcccg
     6361 gattctgaag aatctcgcgg cccatgatga acttctgcag attgccgccc gagaggccgc
     6421 ccgcatgctt ctgcggggac ggggtcttca cgtggaagcg ctcgatgatc ttctccgtga
     6481 agcgcagggc cttctcctcg agaatgaagc cgtgcttcac gaggtcgtcg cccgtgagat
     6541 aggtgttcgt ctggagcgag atttcgggca cggcggcgtg gccgaggcgc tgctcgggca
     6601 cgtagcgtag gccgaggcgg cggcggtcac gggtgccgag gctgccgacg ggcttgccga
     6661 agagcatgac gccctcgtcg gcgacggggg cctcgcccgc agcgaccgcc atcaggcgcg
     6721 cctgaccgtt gcccgagatg ccggcgacgc cgacgatctc gcccttgcgc acggccacgg
     6781 agacgttctc gatgccgcac acgcgcagcg agcctggata gctgacatcc ttcatctcga
     6841 agaccacttc gcccggcgtg cccgaggcct cgcggatcgc gggcggctcg gcgccgatca
     6901 tcagacgggc aagcgacgcc tcggtctcgg ccttcggatt gacggacgag acgatcctgc
     6961 ccgcacgcag cacgacgcag cggtcggcga gctcgcggat ttcgtcaagc ttgtgggaga
     7021 tgaagagaat ggagacgcct tcctcagcca gacggtggag cgtcacgaag agcttcctga
     7081 cggcctgggg cacgagcacc gaggtgggct catcaagaat gaggagcttg ggattcgtca
     7141 tcagggcgcg gatgatctcc acgcgctggc gctcacccat cgagagctcg tagacgaccg
     7201 cagcgggatc cacctcaagg ccgtacttct cgccgagcgt cttcacctcc tcggagattt
     7261 cggccgtgga gcggccgggc ggaagcccga gggccacgtt ctcggccacc gtcagcgtgt
     7321 cgaaaagcgc gaagtgctgg tgcaccatcg caatgccgct cgcacgcgct tcggcaggac
     7381 tcgatacgtc gaggggcctg ccgtcgaact cgaggctccc ctcgtcgggc cgcaccgagc
     7441 cgtagatgat cttcatgagg gtggacttgc cggcgccgtt ctctccgagg acggcgagca
     7501 cttcaccggg cgcgatatcc atgttgatgc cgtcgttggc aacgatcccc gggtaccgct
     7561 tggtgatatt tttcaggctg agacgtggat gcatgctgag gcaggacgca gggtcgggcg
     7621 tccgtgtcaa aaacttgttc ggtccggccc cgccgccgca aggcgggcga ccccggattt
     7681 aaacacgtaa ttttatcgtt ccgcagacac gttgtctaac atcaagcacc ccttataaag
     7741 tcgagccgag aaaacccgcg gttttaactg ccgggatgat aggcgctcaa atccccttcg
     7801 cacaaactcg tgcgaagcat gcgtcctcga
//
LOCUS       DBMDTI010000080         7791 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2514_length_7791_cov_2.135631, whole genome shotgun sequence.
ACCESSION   DBMDTI010000080 DBMDTI010000000
VERSION     DBMDTI010000080.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7791)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2514_length_7791_cov_2.135631"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="ACZPB6_05235"
     CDS             <1..1495
                     /locus_tag="ACZPB6_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR1183910.1"
                     /translation="VKKAATAVKPQTAPAVAESDVRESRHSNRRGRAATPADKRKLFV
                     LDTNVLIHDPMSIFSFAEHDVFLPMTVLEELDNHKKGTSDVARNARSVSRSLDQLMEL
                     NGHDLTQGVPLSLLGNKEATGRLFFETHAVDVPIPAALPTFKGDNRILAVVKGVEGAF
                     PDLAVVLVSKDINMRIKATTLGIPAEDYLSDKVLDDTDMLYSGRMALPSDFWTTVGNS
                     LKSWQDDGTTHYRFETKEAGRYVVNMCLTIEGDPDFMAIVTSVEPDAVTIRLLRDYRT
                     RLKVWGIHARNAEQNMALNLLTDPEIDFVTLLGQAGTGKTLMTLAAALTQTIDERRFS
                     EIIVTRATVSVGEDIGFLPGTEEEKMAPWMGALEDNLEVLHRSENSTGGDWGRQATLD
                     LIKARIRIKSMSFMRGRTFLKKFVIIDEAQNLSPKQMKTLITRAGPGTKIVCLGNIAQ
                     IDTPYLTEGSSGLTYVVERFQGWPHAATITLTRGERSRLADFATEAL"
     gene            1793..6079
                     /locus_tag="ACZPB6_05240"
     CDS             1793..6079
                     /locus_tag="ACZPB6_05240"
                     /inference="COORDINATES: protein motif:HMM:NF045282.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183911.1"
                     /translation="MKACDLEEKTNHLQACVAIDLGARYTGTFSILHKSSRLPSKDDC
                     RAATYVLPEDGGKMTYSQRNRTAVRHRIRGKKRFALARRMAMLIIREKLAAKSVTLTS
                     SETRRLAEAVSSLLRRRGYNRLETETDNDLTVLENIEPEVFSEHEILGKYFYAGTDLA
                     IQWETLSQDLSKISSLLAERLSEKDFKKYLTGLIPDDKNKIKHIISVFKMLIDEAESI
                     NMQCVLGHKHRTQYLSDIHADMHRDTRLSKAAEAFGGVDRLWRLLGNLSNLQLRAHRW
                     YFNAPHLFDDQALEPARLQKTLVRAFKYLHCPQGVESRAQAELVKELEEADDIIDALC
                     TIDPVRTIPPYENQQNRRPPFDQTLYLSPDLLDRQFGHTWKSWAVAFAKEEPLLEDGL
                     EVILAQTDRRSRVKALNKNCRDALDYKFSYVLQRVLDRSKACDSFCLRALAAGAEGSR
                     LTVARNELQRVLGTQHVNDFLRLAKLYYRETGEAKNGLWLQEHACVLERADIHPPMKS
                     KIINQLLGAVFGLDADFGRLFKNKIWNRTVSGRSTVRSMCQNIEQTRKAHGGEFNLLF
                     KQATAGAEGVAEDIAKTVAAVGKLREFFSTIPELDECTINRVANPYSLVQLYTLLEVD
                     RSGFSSVSAAAHFENEWRMKTWKDETCSRCSRLPADTVRPFDGVLRRVLDRQTSELSR
                     QIASDFFQRISGESAQIDLAIVIEENKFAFSASLADLKKNRAAADKAKDALARQEKRW
                     LSKSERIKIAARGLCAYTGEALSDAGEFDHIIPRSQTTAAMGTAFNSEANLLFVSQKG
                     NQQKKDGRYFLTDLKDNYLNRIFGTSDRKQISEEIEKTVDELVQSGRIRHFELLNQKQ
                     QDCVRHALFLNDASEARQAVLRELSATNRSKVNGTQAWFVRSLVSKLTRITADWRNKH
                     NHTLNIRSCRVNAQAASSIRMALGKLAPDLAKPDIQPVASHSIDAMCAYAAACASVDF
                     CSFVGGNPSHSDPESFTNDNQSNLAALYPDACDVRWVKSSSQKDISAKAIFKEGILAE
                     NFLPILVKQDKVYVGFTLPSASGAGGNALPVTGKNPLHLLEVAGPYLDKTPASNSSQT
                     QCCNIVKQKAFALFNKQAQDPDSLTREETEAAALLKHLHYITERIPLSKMLLNDTGNK
                     LLDAAKLKKKIDAQTLSCTIKSKKSQFSVTGTLALPAQVEWARLASDPSLVPHLGESS
                     KTFDLNVWIRSVTHLNAGSKCRHVSVKRIASLPVVSKASGGFRIRRTTATAQEVYQLY
                     NIAGAKCAGFASRNGEVSDWKRPLLKKELVHDNLTPLDVAEYAADESVGLLDFRLVAE
                     ENDITVYIAPGTADRRYVRLELPFPTLRNWLTNAEAVAIPDSPLGLPSEIILKKSKAF
                     APTEPAKVAKLLGTPRSHLFVEQLGSRVRLWYIVESSNSDMSRAFNRAQS"
     gene            6076..7077
                     /gene="cas1"
                     /locus_tag="ACZPB6_05245"
     CDS             6076..7077
                     /gene="cas1"
                     /locus_tag="ACZPB6_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HIR1183912.1"
                     /translation="MKHLSICNFGDCLGVSGNRLTVRSKEGELQEYCLTHLRSICIAK
                     NGISISSNLVQACAARGIRLYFLDWTGKSTACISGEHQHAVVQVRKAQFRAIESVLSH
                     FLSREIILAKLRNQRAVLLYFQKYQAKAFPEKAALFHLAMDRIEHLHDHLKTLDHLMQ
                     TDEWRTVLLGYEGQAAEVYWRTLRTSGLLPSSFQKREGRGSLEVTNAALNYGYSILEK
                     FCWSALENAGLELYAGLLHVDRPGKPSLVLDFMEEYRAWVVDRNIIKLRARLGKETSL
                     TPDLKTEIINGIDATMSAFITHRGKKVRLENIMQRQAYRLAGAMTDGKRYKSIRFKW"
     gene            7082..7378
                     /gene="cas2"
                     /locus_tag="ACZPB6_05250"
     CDS             7082..7378
                     /gene="cas2"
                     /locus_tag="ACZPB6_05250"
                     /inference="COORDINATES: protein motif:HMM:NF021356.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HIR1183913.1"
                     /translation="MSLYVKYLVCYDISNTRTRSKFFDFLKDLGLTPIQKSVFFGDLK
                     TPEVRSLKAAAEKWLDASCDSCLYFPCHLKVEDIRKCFGLKHFTYIEPHENRVI"
     gene            7359..7760
                     /gene="cas4"
                     /locus_tag="ACZPB6_05255"
     CDS             7359..7760
                     /gene="cas4"
                     /locus_tag="ACZPB6_05255"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HIR1183914.1"
                     /translation="MKTESSSIPASLLRQYCFCPRIPFFTYVRQLHPPRAPWVEAGVA
                     MHQRIEQLLKRRDLTKLTSGRSYRLKTEVPLYSPDVPFHGICDAVLELEDGTYIPLEI
                     KSGNASPHTGEIIQLCAYAILLEQLHGGRID"
BASE COUNT         1884 a         2383 c         1978 g         1546 t
ORIGIN      
        1 cgtcaaaaaa gccgcaaccg cagtgaagcc ccagacggcc cccgcagtcg ccgagtccga
       61 cgtgcgcgaa tccaggcact ccaaccgccg cggacgcgcc gcaacgcccg ccgacaagcg
      121 caagctcttc gtgctcgaca ccaacgtgct gatccacgat cccatgagca tcttcagctt
      181 cgccgagcac gacgtcttcc tgcccatgac cgtgctcgaa gagctcgaca accacaagaa
      241 gggcacgagc gacgtcgcac gcaacgcgcg ttcggtgagt cgcagcctcg atcagctgat
      301 ggagctcaac ggacacgatc tgacacaggg cgtgccgctt tcgcttctgg gcaacaagga
      361 agcgacgggc aggctcttct ttgaaaccca cgccgtcgac gtgccgattc ccgccgcact
      421 gccgaccttc aagggcgaca accgcatcct cgcggtcgtc aaaggcgttg aaggcgcctt
      481 ccccgatctg gccgtcgtgc tggtgagcaa ggacatcaac atgcgcatca aggcgacgac
      541 gttgggcatt cccgcggagg actatctctc cgacaaggtg ctcgacgaca ccgacatgct
      601 ctactcgggc cgcatggcgc tgccctccga cttctggacg accgtcggca attccctcaa
      661 gtcctggcag gacgacggca cgacgcacta ccgcttcgag acgaaggagg ccggccgcta
      721 cgtcgtcaac atgtgtctga cgatcgaggg cgaccccgac ttcatggcca tcgtcacgag
      781 cgtcgagccc gacgcggtca cgatccgcct gctgcgcgac taccgcactc gcctcaaggt
      841 ctggggcatt catgcgcgca acgccgaaca gaacatggcg ctcaacctgc tcaccgatcc
      901 cgagatcgac ttcgtcacgc tgctcggtca ggcgggcacg ggcaagaccc tgatgaccct
      961 tgccgccgcc ctcacccaga cgatcgacga gcgccgattc tccgaaatca tcgtgacgcg
     1021 cgcgaccgtc agcgtcggcg aggacatcgg cttcctgccc ggcaccgagg aggagaagat
     1081 ggcgccgtgg atgggcgcgc tcgaggacaa cctcgaggtg cttcaccgct cggaaaacag
     1141 cacgggcggc gactggggtc ggcaggcgac gctcgacctc atcaaggccc gcatccgcat
     1201 caagtcgatg agcttcatgc gcggacgcac cttcctcaag aagttcgtca tcatcgacga
     1261 ggcgcagaac ctgagcccga agcagatgaa gaccctcatc acgcgcgcgg gccccggcac
     1321 gaagatcgtc tgtctgggca acatcgccca gatcgatacg ccctacctga cggaaggcag
     1381 ctccggcctc acctacgtcg tcgaacgctt ccagggctgg ccgcacgcag ccacgatcac
     1441 gctcacgcgc ggcgagcgct cgcgtctggc ggacttcgcg actgaagccc tataaaaaac
     1501 ttgcaaggct gtgaattagg agttatcatt caagtacatc ttaactcaag ccaacatcag
     1561 gaaaacgtct ccggacgtac tgaaatcacc aaaagttaag cttgattccg gccactgtct
     1621 gatgcaagat ccgacatccg ggaagaaaaa ggcgtcgaaa ggcgcctttt ttgttgcctt
     1681 tttccatgct cacaagggaa atccctgaaa aattgacagc agtccaaagc ctagcctatc
     1741 atgctataaa acaatcacta ccctatacaa attccataca tgaggaatca tcatgaaggc
     1801 atgcgactta gaggaaaaaa ccaaccacct tcaagcttgc gttgcgattg accttggcgc
     1861 gcgttacaca ggcaccttct ccatcctgca taagtcgagt cgtctgcctt ccaaagacga
     1921 ctgtcgcgcc gcaacctacg tgctgccgga ggacggcggc aagatgacct attctcaacg
     1981 aaaccgcact gctgtccgac atcgaattcg cggaaagaag cgttttgcac tcgcgcgtcg
     2041 catggccatg ctcatcatcc gcgagaagct ggccgcaaag agcgtcacgc tcacttcttc
     2101 ggaaacccgc cgtcttgctg aagcggtcag cagtctcctg cgccgtcgtg gctacaatcg
     2161 tctcgaaacc gaaaccgaca acgacctgac cgtccttgaa aacatcgagc ctgaagtgtt
     2221 ttccgagcac gagattcttg gaaagtattt ttatgcagga accgatctcg ccatccaatg
     2281 ggaaacacta tcgcaggatc tgagcaaaat ctcctcgctc cttgccgaac gcctttccga
     2341 gaaagacttc aagaagtatc tgacaggctt gattcccgac gacaagaaca agatcaagca
     2401 catcatcagt gtgttcaaaa tgctgatcga cgaagcggaa tcgatcaaca tgcagtgcgt
     2461 tctcggacac aagcaccgca ctcagtacct ttccgacatt catgccgaca tgcatcggga
     2521 cacccgtctg tcaaaggctg ctgaggcttt cggcggcgtg gaccgcctgt ggcggcttct
     2581 gggcaacctc tcgaatcttc agcttcgcgc ccatcgctgg tatttcaacg ctccccacct
     2641 ctttgacgat caggcacttg aaccggccag gctgcagaag acgctggtac gcgcattcaa
     2701 gtatcttcat tgcccgcaag gcgtcgaatc ccgtgcacag gccgaactcg tgaaggagct
     2761 cgaagaagcg gacgacatca ttgacgcact gtgcaccatc gatcccgtcc gcacgattcc
     2821 gccctacgaa aatcagcaaa acagacggcc gcccttcgat cagacgctgt acctgtctcc
     2881 cgatctgctc gacaggcagt tcggacacac ctggaaatcc tgggcagtcg cttttgcaaa
     2941 agaagagccc ctcctcgaag acggacttga ggttattctg gcgcagactg acaggcgctc
     3001 ccgcgtgaag gccttaaaca aaaactgcag ggatgctctg gactacaaat tctcctacgt
     3061 ccttcagcga gttctcgacc gcagcaaggc ctgcgactca ttctgtcttc gtgctctggc
     3121 cgcaggcgct gaaggctcgc gtctgaccgt cgcacgcaat gaactccaac gtgtgcttgg
     3181 aacgcagcac gtgaacgact tcctcagact tgcgaaactc tactaccgcg aaaccggtga
     3241 agccaaaaac ggactctggc tgcaggagca cgcctgcgtg ctcgagcggg cggacattca
     3301 tccgcccatg aaatccaaaa tcattaatca gctgctcggc gccgtcttcg gcctcgatgc
     3361 cgactttgga cgtcttttca aaaacaagat ctggaaccgt accgtcagcg gccgcagtac
     3421 cgtacgttcg atgtgccaaa acattgaaca gacccgcaag gcgcacggcg gcgaattcaa
     3481 tctgctcttc aagcaggcga cggcaggcgc agaaggcgtc gctgaagaca ttgccaaaac
     3541 tgtagcggca gtcggcaagc tgcgtgaatt cttctcgacg attccagagc ttgacgaatg
     3601 cacgatcaat cgagttgcca atccctattc actggttcag ctctacacgc ttctggaagt
     3661 agacagaagc ggcttttcct cagtctcggc agctgcgcat ttcgaaaacg aatggcgcat
     3721 gaagacctgg aaggacgaga cgtgcagcag atgctcgcgt ctgcctgctg ataccgtgcg
     3781 gccgttcgac ggcgttttgc gccgcgtgct cgatcgccag acgagtgaat tgtcccggca
     3841 gatcgcttcc gacttcttcc agcgcatctc cggcgaatcc gcacaaatcg atctagccat
     3901 cgtcattgag gaaaacaagt ttgcattcag cgcctctctg gccgacctga agaagaaccg
     3961 tgccgccgcc gacaaggcca aagacgctct ggcgcgtcag gagaaacgct ggctctccaa
     4021 aagcgaacgc atcaaaattg ctgcgcgcgg cctgtgtgcc tacacgggcg aagcgctcag
     4081 cgatgcaggc gaattcgatc acatcattcc tcgttcccaa acaaccgcag cgatgggaac
     4141 cgccttcaat tccgaagcga acttgctctt cgtttcgcaa aaaggcaacc agcagaagaa
     4201 ggacgggcgc tatttcctga cggatctgaa agacaactat ctgaacagaa tcttcggcac
     4261 ctcggacaga aagcaaatct ccgaggaaat cgagaagacg gtagacgaac tcgtgcagag
     4321 cggacgaatt cgtcattttg agcttctgaa tcagaagcag caggactgcg tccgccacgc
     4381 tctgtttctt aacgacgcgt cggaggcccg tcaggccgtc ctgcgtgaac tgtccgccac
     4441 caaccgcagc aaggtcaatg gaacgcaggc ctggttcgtt cgatcccttg tctcgaagct
     4501 gacccgcata acagcagact ggcgaaacaa gcacaaccat acgctcaaca tcagatcgtg
     4561 ccgagtcaac gctcaagccg ccagcagcat tcgtatggcg ctgggcaagc ttgccccgga
     4621 cctcgccaag cctgacatcc aaccggttgc ctcccattcg atcgacgcga tgtgcgcata
     4681 cgcggccgcc tgcgcgtccg tggacttctg ttcgttcgta ggcggcaatc caagccattc
     4741 ggaccctgaa agcttcacga acgacaatca aagcaatctg gctgccctct atcctgacgc
     4801 atgcgacgtc aggtgggtca agagctcctc gcaaaaggac atctccgcca aagccatttt
     4861 caaagaaggc attttggccg agaatttcct gcctattctt gtcaagcagg acaaggtcta
     4921 cgtcggcttc acgcttccgt ccgcgtcagg tgccggcggc aacgctctgc ccgtcactgg
     4981 caaaaaccct ctgcatctgc tggaggtcgc cggaccgtat ctcgacaaga cgcccgcctc
     5041 gaactcatcg caaactcagt gctgcaacat cgtcaagcag aaggcttttg cgctcttcaa
     5101 caagcaggct caggatcctg actcactgac cagagaagaa acagaagccg ctgcacttct
     5161 caagcacctg cattacatca ccgaaagaat tcctctctcg aagatgctac tgaacgatac
     5221 cggcaacaag cttctggacg ccgccaagct caagaaaaaa attgatgcgc agacgctttc
     5281 ctgcacaatc aagtcaaaga aaagccagtt ctcggtaacc ggcacgcttg cgctcccggc
     5341 gcaggtcgaa tgggccaggc tcgcaagtga tccgtccctc gttccgcatc tcggcgaatc
     5401 cagcaagact tttgacctca acgtctggat tcgctccgtt acgcacctca atgcaggcag
     5461 caagtgcagg cacgtttccg taaagcgcat tgcaagtctt cccgttgtca gcaaggcctc
     5521 aggcggattc agaatccgac gcacgacagc aacagctcag gaggtctatc agctgtacaa
     5581 catcgcaggc gccaaatgcg caggcttcgc atcgagaaac ggtgaagtct cagactggaa
     5641 acgcccgctt ctcaagaaag aactcgttca tgacaatctg acgccgctcg acgtggccga
     5701 atacgccgcc gacgaatccg tcggcctgct cgacttcaga ttggttgccg aagaaaacga
     5761 catcaccgtt tacattgcac ccggcactgc agatcgacgg tatgtacgac tagagctgcc
     5821 gttcccgaca ttgagaaact ggctcacaaa tgctgaagca gtcgctattc cggactcccc
     5881 tctgggactg ccgtcagaga tcattctcaa gaaaagcaag gcgttcgcac cgacagagcc
     5941 tgcgaaagtt gccaaacttc ttggcacacc gcgttcgcat ctcttcgtcg aacagctcgg
     6001 ttcgcgcgtg aggctttggt acatcgtcga atcgagcaac tccgacatgt cacgtgcgtt
     6061 caacagggcc cagtcatgaa acacttgagc atctgcaatt ttggcgattg cctgggagtc
     6121 tcaggcaatc gcctgaccgt gcgttccaaa gagggagagc ttcaggaata ctgcctgact
     6181 cacctccgtt ccatttgcat cgctaaaaat ggcatttcca tttcatccaa ccttgttcag
     6241 gcgtgtgccg caagaggaat ccgcctgtac tttctggact ggacaggaaa atccacagcc
     6301 tgcatctcgg gcgaacacca gcatgcagtt gtgcaggttc gcaaggctca gttccgagca
     6361 atcgagtcag ttctttctca ctttctgagc agagagatca ttcttgcaaa attgagaaat
     6421 caacgtgccg tactgctcta cttccaaaaa tatcaggcaa aggcatttcc tgagaaagct
     6481 gcattgttcc acctcgccat ggatcgcatt gaacaccttc acgaccattt gaagacgctt
     6541 gaccacctca tgcagaccga tgaatggcga acggttcttc tcggatacga aggacaggcc
     6601 gcagaagttt actggcgcac tctgagaaca agcggccttc tcccttcatc gtttcaaaaa
     6661 cgtgagggca gaggctccct tgaagtgaca aatgctgctc tcaactacgg atacagcatt
     6721 cttgaaaagt tctgctggtc tgctttggaa aatgcagggt tggaactcta tgcaggactg
     6781 ctgcacgtcg accgccccgg caaaccctcc cttgtgctgg acttcatgga agagtaccga
     6841 gcctgggtcg tggaccgaaa catcatcaag ctcagagcgc gtctgggaaa ggaaaccagt
     6901 ctgactcccg atctcaaaac cgaaatcatc aacggcatcg atgcaaccat gagtgctttc
     6961 attactcatc gcggcaaaaa ggtccggctc gagaacatca tgcaacgtca agcatatcgt
     7021 ctggccggcg cgatgacgga cggcaagcgc tacaagtcga tcaggttcaa gtggtgaagt
     7081 catgagtctt tacgtcaaat atcttgtctg ctacgacatc tccaatacca gaacccgctc
     7141 gaagtttttc gattttctca aagacctcgg actgacgccg attcaaaaat ccgttttctt
     7201 cggcgatctc aagactccgg aggtacgctc gttgaaagcg gcagctgaaa aatggctcga
     7261 cgcctcctgc gactcctgcc tgtacttccc ctgccatttg aaagttgagg acatcagaaa
     7321 atgctttggc cttaagcact tcacatacat tgagccgcat gaaaacagag tcatctagca
     7381 ttcccgcatc cctactgcga cagtactgct tctgcccacg cattccattt ttcacgtatg
     7441 taaggcaact acatccgccc cgcgcccctt gggtagaagc gggagttgca atgcatcaga
     7501 ggatcgaaca actgctcaag cgaagggatc tcaccaaact cacatcgggg cggtcttaca
     7561 ggctgaaaac ggaagtcccc ctctattctc cggatgtgcc ctttcatggc atctgcgacg
     7621 ctgtactgga acttgaagac ggtacgtaca ttccgctgga gatcaagtcc ggaaacgctt
     7681 ccccgcacac gggcgagatc attcagcttt gtgcatacgc cattttgctt gaacaactgc
     7741 atggcggcag gatcgattga ggcgggtgta gaccaaatcc cgggctcctc t
//
LOCUS       DBMDTI010000081         7715 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2545_length_7715_cov_2.935251, whole genome shotgun sequence.
ACCESSION   DBMDTI010000081 DBMDTI010000000
VERSION     DBMDTI010000081.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2545_length_7715_cov_2.935251"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(163..1284)
                     /locus_tag="ACZPB6_05260"
     CDS             complement(163..1284)
                     /locus_tag="ACZPB6_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR1183915.1"
                     /translation="MKHSILCCAVTGALASGAAFAADTQIYGVIDTGFSVRNNQYVNG
                     NSDTTTRMVSGQYIGSRFGIKGSEDLGNGYKAGFVLESSIKTDDGSLGQGGRLWGREA
                     RVYLSGDFGEVSFGRVGPIVGGNGPYARFGHVMNAFSCGWGDVGGSLQVVSLGYEFVD
                     NAVTYLTPKFGGLDAVFQYSFGADTQSDAYKDGTEGKSSVERMYAGAVRYQNSTVLVS
                     AGVEGINQDQPSADRNGLDDALSFNLGGSYDAGFAKFYVYSQVFQNYAKAAKVTMFSI
                     PSGVDGYGVNLGFETKALGGTVKASFGWGDFEGSHADNLTMKTYQTALGYNYPLSRRT
                     TLYTAAGWIHNDFSDDYAAAKPTAVENDYEWICGITHKF"
     gene            complement(1460..3421)
                     /locus_tag="ACZPB6_05265"
     CDS             complement(1460..3421)
                     /locus_tag="ACZPB6_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1183916.1"
                     /translation="MNKSLSRRAFLRTSIASGASLAAAAAGAAPAGIYRPGTYSAKAS
                     GIGEVTVTMTFDADRIIDVVLDVSHETPGIGQAAADTLKKNLMAAQAAEIDGVSGATI
                     TSKAVSKAAAKCIAQAKGEIPVEVISDKKADEDDGDWLGKEPEIAEKDIVATHETDIL
                     VVGCGTGGLFAVAAAAEAGGKVIGIDRFAVGTGIREDLGAIDSRYQKAWGTKIDKFEF
                     ITMATQYAGGHIQQDLVKLWCDKSGEAIDWVGDRLAERNIELWHESGDKNDETRYKHF
                     AIGHSPKLPIDPKTGKFKITLAQVVEQYAAKKGARFDYNTKMVKLEKKNGRVTGVIAE
                     NADGKYVRYNAAKGVVVATGGYAQNWKMMEALQPWNLRIIGRSGALPGARGDGIRACL
                     WAGAKMDETHSIMMFDRTAIRPDQQPGPDTVKSGDSGFFWIGSQPWLKVNADGRRFMN
                     ESGTYENILHADEYQKGHCHYTIFDSNWVKYAEQFKMHGCSRLFPFENGAEPNIPWEV
                     FRDRMLPGLIAKGYVQKADTIEELAKKLGLPAAQLKATVARNNELYRKGEDVDYGKEK
                     HRLSAVDKAPFYGAKNTGWMLCTMDGIQINTNMNAIDTEGNPIPGLFVIGNDSGGYFA
                     NEYPNLATGMACGRTVTFGRIVGRMLAKA"
     gene            complement(3885..5351)
                     /locus_tag="ACZPB6_05270"
     CDS             complement(3885..5351)
                     /locus_tag="ACZPB6_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-cytosine permease family protein"
                     /protein_id="HIR1183917.1"
                     /translation="MSDNSSASAIEVNGVNPVPDSERYGTPAGLFPIWFSWNISIFGI
                     TLGIYVLGLGLSVWQAMLAGVIGYFVSCALVGILAVGSVRTGLPTLVQSRFAFGYHGN
                     KIPTFFGYVANMGWKVTMLSMASTTLADLMCHLVPMLAQVDGSPTSACVFGSFIVVIV
                     LTMTGAVWGYQLIMKIEKAIAWITGLFTLVYLFFFIPQIDFSVLGDRPSAGIVEFLGA
                     VVLSMTMVGLGFLNYGGDYSRYLPRNTRAGGVIFWTMTGIALPVSALLILGVMLSAGN
                     PELLAKASHEPIAALTGILPFWFYVPFSIVIIVSLISAGMTGVYSSGLALLAMGVPLS
                     RAATTVVNAVIITLGAFYLLFISDSFVSTFQSFLAAISVIMGSWGAIEMVDMLRQKRL
                     NWDVRMAFDYGEGGRSGRWTALLSLAVATIIGLGTITSSDPYIARIVSFLLPSGAETS
                     VFAKANIGLVVAMIAGGLLYAVLTFVCRFDVPPVLKKK"
     gene            complement(5453..6397)
                     /locus_tag="ACZPB6_05275"
     CDS             complement(5453..6397)
                     /locus_tag="ACZPB6_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR1183918.1"
                     /translation="MRSIRSVLHSLKPQKRVLIIGSAFVDMVINVQEMPRAGADVEGD
                     YRCTTVGGCSFNVADVLYKLDLPFESYMPVGEGRFADLVAQALTERGYPVHREAGEGD
                     NGWCLSLADASGERTFISMFGLERRMKPDWFERFDIESFDYFYVSGYQAEGENGRVIL
                     EALSRKRPDAVVVFDPGPRVHFMPRERIEDFCRLGCLFTVNADEALFMTGTTRVEDAA
                     LRLHDMSGRDAVVTDGGRGAIVADNASVRRIPGFPVAIVDTIGSGDAHTGGVIAGLMC
                     GLPIDECVLLANRVASVVTGREGGACAPTIDELLTEHC"
     gene            complement(6410..7411)
                     /locus_tag="ACZPB6_05280"
     CDS             complement(6410..7411)
                     /locus_tag="ACZPB6_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1183919.1"
                     /translation="MTIENRIAGALAGMVLGDALGVPSELWPREKVRARFGHIDTFLD
                     GPEDNIVACYFKAGHYTDDSAQAFVILKALLKAGTVPPVKVLADDLIAWVESMNGFEI
                     NLLGPSSKASLLAHSRGEDYTKYTKLSLTNGAGMRIAPVGCLVDWNDSEKLAETVARV
                     SIVTHGTDVAIGGAAMVAQAVASGIGGRSWEEAAEDALRIWNVARAKGEPTWAASVAE
                     RFRLALSVMKDFDDDEKFSRWVYDILGTGTMTSESVSAALAVAFYCRSAERAAIMCAN
                     MGGDTDTIGAMACAICGAFEGFDALPESRSSFLETTNALDFRAMAAEIARARTTFHL"
BASE COUNT         1307 a         2164 c         2829 g         1415 t
ORIGIN      
        1 aaaggaggtg ttgcggccgc ttcatcattc ccgtgaagga ctgatccgga gtgtggcgcg
       61 gatgaaaaca aacgggggcg gccgtgaggc cgtccccgct tgcttctggt tttttctgcc
      121 tgtcagctca ggccttcgtc ggtcagaacc ggttgttctt catcagaact tgtgcgtgat
      181 gccgcagatc cattcgtagt cgttttcgac ggcggtgggc ttggcggccg catagtcgtc
      241 ggagaagtcg ttgtggatcc agccggcggc cgtgtagagc gtcgtgcggc gggagagcgg
      301 gtagttgtag ccgagggccg tctgataggt cttcatcgtc aggttgtcgg cgtggctgcc
      361 ttcgaagtcg ccccacccga aggaggcctt gaccgtgccg ccgagagcct tcgtttcgaa
      421 gccgaggttc acgccgtagc cgtcgacgcc ggagggaatg gagaacatcg tgaccttggc
      481 ggccttggcg taattctgga agacctggct gtagacgtag aacttggcga agccggcatc
      541 atagctgccg ccgaggttga aggagagtgc atcgtcgagg ccgttgcggt cggcgctcgg
      601 ctgatcctga ttgatgcctt caacgccggc ggacacgagg accgtgctgt tctgatagcg
      661 gacggcgcct gcgtacatgc gctcgacgct ggacttgcct tccgtgccgt ccttgtaggc
      721 gtcggactgc gtgtcggcgc caaaggagta ctggaagacg gcgtcaaggc cgccgaactt
      781 gggcgtaagg taggtgacgg cgttgtcgac gaattcgtag ccgagcgaga cgacctgaag
      841 gctgccgccg acgtcgcccc agccgcagga gaatgcgttc atgacgtggc cgaagcgggc
      901 gtacggaccg ttgccgccga cgatcgggcc gacgcggccg aagctgactt cgccgaagtc
      961 gccggaaagg tagacgcggg cttcgcggcc ccagaggcgg ccgccctgac cgagggagcc
     1021 gtcgtcggtc ttgatgctgc tttcgaggac gaagccggcc ttgtagccgt tgccgaggtc
     1081 ttcggagccc ttgatgccga agcgggagcc gatgtactgg ccggagacca tgcgggtggt
     1141 ggtgtcgctg ttgccgttga cgtactggtt gttgcgaacg gaaaaaccgg tgtcgatgac
     1201 gccgtagatc tgagtgtcgg cggcgaaggc ggcgccggag gcgagggcgc cggtcacggc
     1261 gcagcagagg atggaatgct tcatatcagt tcgggcgtcc tgcgtgcggg atcggcgcgg
     1321 cgcccttgtc ctgttgtggt tttgaggaag gttgaagatg atgggggcgg gccgccggag
     1381 gattgttccc tctcttgcgg ccctggaggc atccgaggtg agctgccttt ttcgatgcgt
     1441 gccctttccg tgcgcgggat caggccttgg cgagcatgcg gcccacgatt cggccaaagg
     1501 tcaccgtgcg gccgcaggcc atgccggtgg cgaggttcgg gtactcgttg gcgaagtagc
     1561 cgccgctgtc gttgccgatg acgaagaggc ccgggatcgg gttgccctcg gtgtcgatgg
     1621 cgttcatgtt ggtgttgatc tggatgccgt ccatggtgca gagcatccag cccgtgttct
     1681 tggcaccgta gaagggagcc ttgtcgacgg cggagagacg gtgcttttcc ttgccgtagt
     1741 cgacgtcctc acccttgcgg tagagctcgt tgttgcgggc gaccgtggcc ttgagctggg
     1801 cggccggaag accgagcttc ttggcgagct cttcaatcgt gtcggccttc tggacgtagc
     1861 ccttggcgat caggccaggg agcatgcggt cacggaagac ctcccaggga atgttgggct
     1921 cggcgccgtt ttcgaacggg aagaggcgag agcagccgtg catcttgaac tgttcggcgt
     1981 acttgaccca gttggagtcg aagatcgtgt agtggcagtg gcccttctga tattcgtcgg
     2041 cgtggaggat gttctcgtag gtgccggatt cgttcatgaa gcgcctgccg tcggcgttga
     2101 ccttgagcca gggctgggag ccgatccaga agaagccgct gtcgccggac ttgaccgtgt
     2161 cggggccggg ctgctggtcg ggacggatgg cggtgcggtc gaacatcatg atcgagtgcg
     2221 tctcgtccat cttggcgccg gcccagaggc aggcgcggat gccgtcgccg cgggcgccgg
     2281 gcagggcgcc ggagcggccg atgatgcgga ggttccaggg ctgcagggcc tccatcatct
     2341 tccagttctg ggcgtagccg ccggtggcga ccacgacgcc cttcgcggcg ttgtagcgga
     2401 cgtacttgcc gtcggcgttc tcggcgatga cgccggtcac gcggccgttc ttcttctcga
     2461 gcttgaccat cttcgtgttg tagtcgaagc gggcgccctt cttggcggcg tactgctcga
     2521 cgacctgagc gagcgtgatc ttgaatttgc ccgtcttcgg atcgatcggg agcttcggag
     2581 agtggccgat ggcgaagtgc ttgtagcggg tttcgtcgtt cttgtcgccg gactcatgcc
     2641 agagctcgat gttgcgctcg gcgaggcggt cgccgaccca gtcgatggct tcgccggact
     2701 tgtcgcacca gagcttgacg aggtcctgct ggatgtggcc gcccgcgtac tgcgtggcca
     2761 tcgtgatgaa ctcgaacttg tcgatcttgg tgccccaggc cttctggtag cgggagtcga
     2821 tggcgccgag gtcctcgcgg atgcccgtgc cgacggcgaa tcggtcgatg ccgatcacct
     2881 tgccgcccgc ttcggcagcg gcggcgacgg cgaagaggcc gcccgtgccg cagccgacga
     2941 cgagaatgtc ggtctcgtgc gtggcgacga tgtccttttc ggcgatttcg ggctccttgc
     3001 cgagccagtc gccgtcgtct tcgtcggcct tcttgtcgga gatgacttca acgggaattt
     3061 cgcccttggc ctgcgcaatg cacttggcgg cggccttgga gacggccttc gacgtgatcg
     3121 tggcgccgga gacgccgtcg atttcggcgg cctgagcggc catcaggttc ttcttgagcg
     3181 tgtcggcggc ggcctgaccg atccccgggg tttcgtggga gacgtcgagc acgacgtcga
     3241 tgatgcggtc ggcgtcaaag gtcatcgtga ccgtgacttc gccgatgccg gaggccttgg
     3301 cggaatacgt gcctggcctg tagatgccgg cgggagccgc accagcggcc gcagcggcga
     3361 gagaggcgcc gctcgcaatg ctcgtgcgga ggaatgcacg acgggagaga gacttgttca
     3421 tgatgagttc cgaatcgaat gggacccgca gggagaggct gcgggtttgc cgtaaagggg
     3481 cgcaggagaa ggagaacctg cgggcgccct tggaaatgaa atgacctggg cgcaactcta
     3541 cgggtgcgtg tctgattggg gtcaagtacc tctcaagagg taggtttgag gccgtttccg
     3601 ggcttttctt taggtgaaat cccgaatgct ttggcgaggc aaacggccgc gtgcagggag
     3661 gctcggacgt cggcgtatgg cgggtatggt tggaaacgcc tgcggcactg ggaagagcgg
     3721 cgtgcgctcg atggaagttg agggaagggc tcgacttcgg ggccctgagg acgctgcggg
     3781 cgggtagcgg gcgcaggatt agtggacgag gatcaagaaa aggccccgcg tttacataag
     3841 cgcggggcct tccggaggat ttcagcctga atgaatgggc gggattattt ctttttcagg
     3901 acgggcggga cgtcgaagcg gcagacgaag gtgaggactg cgtagagcag gccgcccgcg
     3961 atcatggcga cgacgaggcc gatgttggcc ttggcgaaga cgctcgtctc ggcgcccgag
     4021 ggcaggagga agctcacgat tcgggcgatg taggggtcgc tcgacgtgat ggtgcccaga
     4081 ccgatgatcg tggcgacggc gagcgagagg agtgcggtcc agcggccgct gcggccgccc
     4141 tcgccgtagt cgaaggccat gcggacgtcc cagttgaggc gcttctggcg cagcatgtcg
     4201 accatttcaa tggcgcccca ggagcccatg atcacggaga tcgctgcgag gaacgactgg
     4261 aaggtcgaga cgaaggagtc cgaaatgaag agaagataga aggcgccgag cgtgatgatc
     4321 acggcgttga cgacggtggt tgcggcgcgg gagagcggca cgcccatggc gaggagcgcg
     4381 aggcccgagc tgtagacgcc cgtcatgccg gccgagatga ggctcacgat gatgacgatt
     4441 gagaacggta cgtagaacca gaagggaagg atgccggtga gcgcggcgat cggttcgtgg
     4501 gaggccttgg cgagaagctc ggggttgccc gcagagagca tgacgccgag gatgaggaga
     4561 gccgagacgg gaagtgcgat gcccgtcatc gtccagaaaa tgacgccgcc cgccctcgtg
     4621 ttgcggggca ggtagcggga gtagtcgccg ccatagttga gaaagcccag gcccaccatc
     4681 gtcatggaaa gcacgacggc accgaggaat tcgacgatgc ctgcggacgg acggtcgccg
     4741 aggacggaga agtcgatctg cgggatgaaa aaaaagaggt agacgagcgt gaagaggccc
     4801 gtgatccatg cgatcgcctt ctcgatcttc atgatgagct ggtagcccca gacggccccc
     4861 gtcatcgtga ggacgatcac gacgatgaag gagccgaaga cgcaggcgga cgtgggggag
     4921 ccgtcgacct gtgcgagcat gggaacgagg tggcacatca ggtcggcgag cgtcgtcgag
     4981 gccatcgaga gcatcgtgac tttccaaccc atgttggcca cgtagccgaa gaaggtcggg
     5041 atcttgttgc cgtggtagcc gaacgcgaag cgcgactgca cgagcgtggg cagccccgtg
     5101 cgtacggaac ccacggcgag gatgcccacg agggcgcagg agacgaagta gccgatcacg
     5161 cctgcgagca tggcctgcca gacggagaga ccgagcccga gcacgtagat gccgagcgtg
     5221 atgccgaaga tggagatgtt ccaggagaac cagatgggaa agagcccggc gggcgtgccg
     5281 tagcgttcgc tgtcgggaac ggggttgacg ccgttgacct caatggcgga ggcggaggag
     5341 ttgtcgctca tgttggttgt cttttcttga ggaagggatg aaattcccgg ttcggcccag
     5401 gggaaggccg ggcggggcgg ttgatttgaa tgggaatgag ggcccttgta cgtcagcagt
     5461 gctcggtgag gagctcgtcg atcgtgggcg cgcaggcgcc gccctcgcgc cccgtcacga
     5521 cggaggccac gcggttggcg agcagcacgc attcgtcgat cgggaggccg cacatgaggc
     5581 ctgcgatcac gccgcccgtg tgtgcgtcgc ccgagccgat cgtgtcgacg atcgcgacgg
     5641 gaaagcccgg gatgcggcgc acgctggcgt tgtcggccac gatcgcgccg cggccgccgt
     5701 cggtgacgac ggcatcgcgt cccgacatgt cgtgcaggcg cagtgcggca tcctcgaccc
     5761 gggtcgtgcc cgtcatgaaa agggcctcgt cggcgttgac ggtgaagagg cagccgaggc
     5821 ggcagaagtc ctcgatgcgc tcgcgcggca tgaagtgcac gcggggaccc ggatcgaaca
     5881 ccacgacggc gtctgggcgc ttgcgggaga gggcctcgag gatgacgcgg ccgttttcgc
     5941 cctcggcctg gtagcccgag acgtagaagt agtcgaagct ctcgatgtcg aagcgctcga
     6001 accagtcggg cttcatgcgg cgctcaaggc cgaacatcga gatgaaggtg cgctcgccgg
     6061 aggcgtcggc aagcgacagg caccagccgt tgtcgccttc gcccgcctcg cggtgcacgg
     6121 gatagccgcg ctccgtgagg gcctgcgcga cgaggtcggc gaaccggccc tcgcccacgg
     6181 gcatgtagct ctcgaatgga aggtcgagct tgtagaggac gtccgcgacg ttgaaggagc
     6241 agccgccgac ggtcgtgcag cggtagtcgc cctcaacgtc ggcgcccgca cgcggcattt
     6301 cctgaacgtt gatcaccatg tcgacgaagg ccgagccgat gatgaggacg cgcttttgag
     6361 gcttgagcga atggaggacg gagcggatgc ttcgcatgca ggggctccct cagaggtgaa
     6421 aggtcgtgcg ggcgcgggcg atttcggccg ccatggcgcg gaagtcgagc gcgtttgtcg
     6481 tctcgaggaa ggacgagcgc gattcgggca gcgcatcgaa tccctcgaag gcaccgcaga
     6541 tcgcgcaggc catcgcaccg atcgtgtcgg tgtcgccgcc catgttggcg cacatgatcg
     6601 cggcgcgttc ggcgctgcgg cagtagaagg cgacggcaag cgcggcgctc acgctctcgc
     6661 tcgtcatcgt gcccgtgccg aggatgtcgt agacccagcg ggagaacttc tcgtcgtcgt
     6721 caaaatcctt catgacggag agtgcgaggc ggaagcgttc cgcgaccgaa gctgcccagg
     6781 tgggctctcc cttggcgcgc gcaacgttcc agatgcggag cgcgtcctct gcggcttcct
     6841 cccaggagcg tccgccgatg ccgctcgcga cggcctgggc gaccatggcc gcgccgccga
     6901 tcgccacgtc cgtgccatgc gtgacgatcg agacgcgcgc aaccgtttcg gcgagctttt
     6961 cggaatcgtt ccagtcgacg aggcagccca cgggcgcaat gcgcatgccc gcgccgttcg
     7021 tgagcgagag cttggtgtac ttcgtgtagt cttctccccg ggaatgggcg agaaggcttg
     7081 ccttggaact cgggccgagc aggttgattt cgaacccgtt catcgactcg acccaggcga
     7141 tcagatcgtc ggcgagcacc ttgacgggcg gcaccgtgcc ggccttgaga agcgccttga
     7201 ggatcacgaa ggcctgtgcg gaatcgtccg tgtagtggcc ggccttgaag tagcaggcga
     7261 cgatgttgtc ctcggggccg tcgaggaacg tgtcgatgtg cccgaagcgg gcgcggacct
     7321 tctcgcgggg ccacagttcg ctcggcacgc cgagggcgtc gccgagcacc atgccggcca
     7381 gagcgccggc gattctgttt tcaatcgtca tgtcttgtgc gttcgaaagt ggcggtgggc
     7441 cctcgcgcac gcgcgcgacg gaccgcagga ttttcggagc cgaattgtag cggaagtcgg
     7501 cctttgcgtc acctgtcgaa tgcgcgggtt tcgttgtgcg agtgtcgttt taaagcgact
     7561 ggcgcatgtg tacgattgag tgggagtggg tttcggaggg gcgtggcggg ggcggaaatg
     7621 aagacggggc cgcgtgcagt ctctgcccgc ggccccgtcc gccggtccta tggctgccgg
     7681 aacaatgtcc ggggaccatc tcggcgccgc cgcag
//
LOCUS       DBMDTI010000082         7672 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2559_length_7672_cov_2.327302, whole genome shotgun sequence.
ACCESSION   DBMDTI010000082 DBMDTI010000000
VERSION     DBMDTI010000082.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2559_length_7672_cov_2.327302"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="ACZPB6_05285"
     CDS             <1..425
                     /locus_tag="ACZPB6_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013717.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR1183920.1"
                     /translation="GEPSKPGEYRLVSPKSAWRLHSQLDAVTRRAHNMDGCEPCWINE
                     DDAARLGISTGDAVRLSTPRGSVRAAAVVTDRVRPGVLALHHGAWAETTEAGEREDGG
                     VRLDCRHGAANVLVPDLPTSGWSRGNNAAAYDVRLERL"
     gene            complement(527..757)
                     /locus_tag="ACZPB6_05290"
     CDS             complement(527..757)
                     /locus_tag="ACZPB6_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183921.1"
                     /translation="MQEGMFLQKSAVAKGTVLKERRGEREEGRKEGSAPELEPGALGG
                     PGGERAANRALITVINEMLAANGDGVGRNARR"
     gene            1005..2591
                     /gene="leuA"
                     /locus_tag="ACZPB6_05295"
     CDS             1005..2591
                     /gene="leuA"
                     /locus_tag="ACZPB6_05295"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR1183922.1"
                     /translation="MIYTDPKRIILFDTTLRDGEQALPQSLSAREKMQIALALEQLGV
                     DVIETGFPVSSRGDFESVRSIASVLKRAVPCGLSRAVAGDIDACAEALKAAERFRIHT
                     FIATSDIHVRDKLRKDFDRILEMGVEAVRRARNYTDDVEFSCEDAGRTPIDHLCRMVE
                     AAIDAGATTVNIPDTVGYTVPEEFGGIITTLFNRVPNIDRAIISVHCHNDLGMATANS
                     ITAIQHGARQIECCMNGLGERAGNCSLEEVAMAIKLREKSLGGLYTGINPKQIARTSG
                     LVSKICSEPVPAHKAIVGSNAFAHSSGIHQDGVLKNRETYEILTPESVGFTGNTMRMT
                     ARSGRHMIRASLANMGYKDDEYDLNEIYARFLKLADKKGQVFDYDLEALLFCSTLEEE
                     ENTFELDALSVMTGGSHSIPTASVRLRVGKRTRSDSSIGNGPVDAVYNCITRLTGIRC
                     ELTSFTISANGAGMDALGQVDVNVRHEGRVFHGIGMSVDILEAAALALIHASNNIDRA
                     NRIRESRRHEKDADVPQHLP"
     gene            2651..3751
                     /gene="leuB"
                     /locus_tag="ACZPB6_05300"
     CDS             2651..3751
                     /gene="leuB"
                     /locus_tag="ACZPB6_05300"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR1183923.1"
                     /translation="MQTRTHHVAVLAGDGIGPEVMAEALKVMDAAAKKFGFTVTRDER
                     LVGGAAIDACGRPLPDETVEACDRTDAILFGSVGGPKWDALPHESRPEVGALLPLRKR
                     YELFCNLRPARFYEGLEGLSPLRRDIAEKGFDMVCVRELTGGIYFGRPKGREGEGEAE
                     RAFDTETYTRAEVRRIAKLAFEIARHRRGRVTSVDKANVLATSRLWRETVEEVAADHP
                     DVTLEHMYVDNATMQLLKAPSQFDVVLCSNMFGDILSDECAMVTGSMGMLPSASLGGS
                     GFGLYEPAGGSAPDIAGKGIANPIAQILSAAMMLRHSLGESEAAACIEAAVAKVLKSG
                     VITGDLASAMPGATVVGTAAMGDAVARAVEEA"
     gene            3754..5154
                     /gene="leuC"
                     /locus_tag="ACZPB6_05305"
     CDS             3754..5154
                     /gene="leuC"
                     /locus_tag="ACZPB6_05305"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR1183924.1"
                     /translation="MGRTLYEKVYDAHVVNELSGELPLLYIDRHLLHEVTSPQAFSGL
                     REAGRKLRRPDLMLATMDHDISTQKATLDVCSPMAREQVTTLIRNCREFGVTLFGLGH
                     PGQGIVHIMGPQTGFTLPGTTLVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLKQ
                     QRFKTMRIVCDGVLPKGVYAKDLILAIAARITTAGGTGYAIEFAGTGVEALSMEGRMT
                     LSNMAIEVGAKVGMIAPDETTFAYLKGRPFAPKGEAWDAAVEYWRTLRSDDDAVFDKE
                     VHIDCSSLEPHVTWGTNPGQAMPVTGRVPVLAEMADPVERAGAEAALAYIGVEPGAPV
                     TDAKVDTVFIGSCTNGRLEDFREAARVLKGRRVADGVRALAVPGSMAVRAAAEAEGLD
                     RIFIEAGFEWRLPGCSMCLAMNDDRATEGSRVASTSNRNFVGRQGRGSRTHLMSPASA
                     AAAAVAGHIADVRDYI"
     gene            5169..5804
                     /gene="leuD"
                     /locus_tag="ACZPB6_05310"
     CDS             5169..5804
                     /gene="leuD"
                     /locus_tag="ACZPB6_05310"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR1183925.1"
                     /translation="MEKFTTLTGVAAPLDAANVDTDQIIPKQFLLAVDRKGFGVHLFH
                     ERRYLDDAETIENPDFVLNKPAYRSASILVARRNFGNGSSREHAPWTLLDFGIRAIIA
                     PSFADIFSNNALGNGLVLVNLAEEEVDELFRILEADPGMKVTVDLVTMTCTAGERTWR
                     FTLDPFRRKCLLEGLDAVSLTLEHEDDIRAREKREPAWLVPHVEDLKSCPV"
     gene            complement(5785..6042)
                     /locus_tag="ACZPB6_05315"
     CDS             complement(5785..6042)
                     /locus_tag="ACZPB6_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183926.1"
                     /translation="MPMQTARLFEERDGIVAIPIHLGPESNRGRGAFDTRLIRHERVA
                     DEPALQWLRGLFAVHAHREDPKAKTGAFRAFRRRRFRPDRT"
     gene            6976..>7672
                     /locus_tag="ACZPB6_05320"
     CDS             6976..>7672
                     /locus_tag="ACZPB6_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HIR1183927.1"
                     /translation="MAARKEFPVKVRRVAVTNKKTGVKYIEERRYQYDPAKGYNVLLS
                     SRRTGEKILEGETVTTRCRPKKKPAEAAQTAELSAKRTRVGALDLIRHAGAVAGLESS
                     VRRAYPNGGTSEKLLSVSQYLVATGETVHNVEAWQCEHDLPYEAGLSEDICDDLFHEL
                     GLDESGSQSLFRELARTAGNDEQPAIAFDSTSHSVYGNGLKPYARQGFNKDGDGLDIY
                     KIITFYSLDSGLPV"
BASE COUNT         1453 a         2548 c         2358 g         1313 t
ORIGIN      
        1 tcggcgagcc ctcgaagccg ggcgaataca ggctcgtgag tccgaagtct gcctggcggc
       61 ttcacagcca gctcgacgcc gtgacgcgca gggcgcacaa tatggacggg tgcgagccgt
      121 gctggatcaa tgaggacgat gcggcgaggc tcggcatctc gacgggcgac gccgtgaggc
      181 tttcgacgcc gagaggctcg gtgcgcgcgg ctgcggtcgt caccgacagg gtacgccccg
      241 gcgtgcttgc gctccatcac ggcgcatggg ccgagacgac ggaggcgggc gagcgtgagg
      301 acggcggcgt gaggctcgat tgccgccacg gcgccgccaa cgtgctcgtc cccgacctgc
      361 ccacctcggg ctggagccga ggcaacaacg ccgcagccta cgacgtgagg ctcgagcgcc
      421 tctgaccgcc cgcccgtgag gggacgcatg ttttcagagg tcgtgctagc atccgaacaa
      481 cggacgcgca cccgaaggtt cgtcgccggc gtttcgccgg cgctgctcaa cggcgggcat
      541 ttcgcccgac cccgtctccg ttcgcggcga gcatctcgtt gatcacggtg atcaacgctc
      601 tgttcgccgc ccgttcgccg cccggacctc caagggcccc gggttcaagt tcgggtgcgc
      661 ttccttcctt ccttccttcc tctctctctc ctctcctctc cttcagaacc gtgcctttcg
      721 cgacggcgga tttttgcaaa aacatgcctt cctgcatttt tgatgtcttg caaaagcgtc
      781 actttttgca gaaaatcaaa ctaggtgaaa ttccctaagc acaaattcat ttctcattaa
      841 aatcaacgaa ttggcaataa ccatgtgatt tttgcggttt ttgcaattga cgtcggtttt
      901 gcgaagacat aaagttgtct tgccgccctt cgtgacgcgc attgtgaaga tgcggtccgg
      961 ggcttcgaac ccagttcaac gcacaacgac aaagagccga cctcatgatc tacacggatc
     1021 ccaagcgcat catccttttc gacaccacgc tccgcgacgg cgagcaggcc ctgcctcaga
     1081 gcctctccgc acgcgagaag atgcagatcg cactcgccct cgagcagctc ggggtcgacg
     1141 tgatcgagac gggctttccc gtatcctccc gcggcgactt cgagagcgtg cgcagcatcg
     1201 cttccgttct caagcgtgcg gtgccctgcg gcctctcccg cgccgtggcg ggcgacatcg
     1261 acgcctgtgc cgaggcgctc aaggcggccg agcggttccg catccacacc ttcatcgcca
     1321 cgtccgacat tcacgtgagg gacaagctgc gcaaggactt cgacaggatc ctcgagatgg
     1381 gcgtcgaggc cgtcaggcgc gcccgcaact acacggacga cgtcgaattt tcctgcgagg
     1441 acgcgggccg cacgccgatc gaccatctct gccgcatggt ggaggccgcg atcgatgcgg
     1501 gcgccacgac cgtcaacatt cccgacaccg tgggctacac ggtgcccgag gagttcggcg
     1561 gcatcatcac gacgctcttc aaccgcgtgc ccaacatcga cagggcgatc atttcggtgc
     1621 actgccacaa cgacctcggc atggccacgg ccaactcgat caccgccatt cagcacggcg
     1681 cgcgccagat cgagtgctgc atgaacggtc tgggcgagcg tgcgggcaac tgctcgctcg
     1741 aggaggtcgc catggcgatc aagctgcgcg agaagtcgct cggcggtctc tacacgggca
     1801 tcaacccgaa gcagatcgcc cgcacctcgg gcctcgtctc caaaatctgc agcgagcccg
     1861 tgcccgcgca caaggcgatc gtgggctcca acgccttcgc gcactcgtcg ggcattcatc
     1921 aggacggcgt cctcaagaac cgcgagacct acgagatcct cacgcccgag agcgtgggct
     1981 tcacgggcaa cacgatgcgc atgacggcgc gctcgggccg ccacatgatc cgcgcctcgc
     2041 tcgccaacat gggctacaag gacgatgaat acgatctgaa cgaaatctac gcgcgcttcc
     2101 tgaagctcgc cgacaagaag ggccaggtct ttgactacga cctcgaggcg ctcctcttct
     2161 gctccacgct cgaagaggag gagaacacgt tcgagctcga tgccctgagc gtgatgacgg
     2221 gcggctcgca ctcgatcccg accgcctccg ttcgcctgcg cgtcggcaag cgcacccgca
     2281 gcgactcctc gatcggcaac ggtcccgtcg acgcggtcta caactgcatc acgcgcctca
     2341 ccggcatccg ctgcgaactt acgagcttca cgatctccgc caacggcgca ggcatggacg
     2401 cactcggcca ggtcgacgtc aacgtgaggc acgagggccg cgtcttccac ggcatcggca
     2461 tgtcggtcga cattctcgag gccgccgcgc tcgccctgat ccacgccagc aacaacatcg
     2521 accgcgcgaa ccgcatccgc gagtcgcgcc gccatgagaa ggacgcggac gtgccgcagc
     2581 acctgccctg attcatcagc atcaaggtca accccaaaac tcaacgacag catcaaaaga
     2641 gacaagcatc atgcagacac gcacgcatca cgtcgccgtc ctcgccggcg acggcatcgg
     2701 ccccgaagtc atggccgagg cgctgaaggt catggacgca gcggcgaaga aattcggctt
     2761 cacggtcacc cgcgacgagc gcctggtcgg cggcgccgcc atcgacgcct gcggcaggcc
     2821 gctgcccgac gaaaccgtcg aggcctgcga caggaccgac gccattctgt tcggctccgt
     2881 gggcggcccg aagtgggacg cgctcccgca cgagtcccgc cccgaagtgg gcgcgctgct
     2941 tcccctgaga aagcgctacg aactcttctg caacctgcgt ccggcccgct tctacgaagg
     3001 cctcgagggc ctctcgcccc tgcgccgcga catcgccgaa aagggcttcg acatggtctg
     3061 cgtgcgcgag ctcacgggcg gcatctactt cggtcgtccg aagggccgcg aaggcgaggg
     3121 cgaggccgaa cgtgcgttcg acaccgaaac ctacacccgc gccgaagtga ggcgcatcgc
     3181 gaagctcgcc ttcgagatcg cccgccaccg ccgcggccgc gtgacgtccg tcgacaaggc
     3241 caacgtcctc gccacgtccc gcctctggcg cgagaccgtc gaggaggtcg cggccgacca
     3301 tcccgacgtg acgctcgagc acatgtacgt cgacaatgcc acgatgcagc tcctcaaggc
     3361 gccctcgcag ttcgacgtcg tgctctgctc caacatgttc ggcgacatcc tctccgacga
     3421 atgcgccatg gtgacgggct cgatgggcat gctcccgtcg gcttcgctcg gcggctcggg
     3481 cttcggcctc tacgagcccg cgggcggctc cgcgcccgac atcgcgggca agggcatcgc
     3541 caatccgatc gcccagatcc tctcggccgc catgatgctg cgccattccc tcggcgaatc
     3601 cgaggccgcc gcctgcatcg aggccgccgt cgcgaaggtc ctgaagtccg gcgtcatcac
     3661 gggcgacctg gcctcggcca tgccgggcgc cacggtcgtc ggcaccgccg ccatgggcga
     3721 cgccgtcgcc cgcgccgtcg aggaggcctg atcatgggaa ggacgctcta cgaaaaggtc
     3781 tacgacgccc acgtcgtcaa cgagctgtcg ggcgaactgc cgctcctcta catcgaccgc
     3841 catctgctcc atgaggtcac ctctccgcag gccttctcgg gcctgaggga ggcgggcagg
     3901 aagctacgcc gtcccgacct gatgctcgcg acgatggacc acgacatctc gacgcagaag
     3961 gcgacgcttg acgtctgcag tcctatggcg cgcgagcagg tgacgaccct gatccgcaac
     4021 tgccgcgagt tcggcgtgac gctcttcggc ctgggccatc ccggccaggg catcgtgcac
     4081 atcatgggtc cgcagacggg cttcacgctg ccgggcacta cgctcgtgtg cggcgacagc
     4141 cacacggcca cgcacggcgc cttcggggca ctcgcgttcg gcatcggcac gtccgaggtc
     4201 gagcacgtga tggcgaccca gaccctcaag cagcagcgct tcaagacgat gcgcatcgtc
     4261 tgcgacggcg tcctcccgaa gggcgtctac gccaaggacc tgatcctcgc gatcgccgca
     4321 aggatcacga ccgcaggcgg cacgggctat gcgatcgaat tcgcgggcac gggcgtcgag
     4381 gcgctctcga tggaaggacg catgacactc tccaacatgg ccatcgaggt gggcgccaag
     4441 gtgggcatga tcgctcccga cgagacgacg ttcgcttatc tgaagggccg tcccttcgct
     4501 ccgaagggcg aggcctggga cgccgccgtc gagtactggc gcacgctgcg ctcggacgac
     4561 gacgccgtct tcgacaagga ggttcacatc gactgttcga gcctcgagcc gcacgtgacc
     4621 tggggcacga atccgggaca ggccatgccc gtgacgggca gggtgcccgt tctcgccgaa
     4681 atggccgatc ccgtcgagcg tgcgggcgcc gaagccgcgc tcgcctacat cggcgtcgag
     4741 ccgggtgcgc ccgtcaccga cgcaaaggtg gacacggtct tcatcggctc gtgcacgaac
     4801 ggacgtctcg aggacttccg cgaggcggcc agagtactga agggccgcag ggttgccgac
     4861 ggcgttcgcg ccctggccgt gccgggctcc atggccgtgc gcgccgcggc cgaggccgag
     4921 ggactcgacc ggatcttcat cgaggcaggg ttcgagtgga ggcttccggg ctgctcgatg
     4981 tgccttgcca tgaacgacga ccgcgccacc gagggctcgc gcgtcgcctc cacctccaac
     5041 cgcaacttcg tcggccgcca gggacgcggc agccgcaccc atctgatgag ccccgcatcg
     5101 gccgccgccg cagccgttgc cggccacatc gccgacgtcc gcgattacat ttgaggaggc
     5161 gcgcagaaat ggaaaagttc acgactctga cgggcgtcgc cgccccgctc gacgccgcca
     5221 acgtcgacac cgaccagatc atcccgaagc agttcctgct tgcggtcgac cgcaagggct
     5281 tcggcgtgca tctctttcac gagcgccgct atcttgacga cgccgagact atcgagaatc
     5341 ccgacttcgt gctcaacaag ccggcctaca ggagcgcctc gatcctcgtc gcgcgccgca
     5401 acttcggcaa cggctcgagt cgcgagcacg cgccctggac gctgctcgac ttcggcatcc
     5461 gcgccatcat cgcgccgtcc ttcgccgaca tcttcagcaa caacgcgctc ggcaacgggc
     5521 tcgtgctggt gaacctcgcc gaggaggagg tcgacgagct cttcaggatc ctcgaggccg
     5581 atcccggcat gaaggtgacg gtcgatctcg tcacgatgac ctgcacggcg ggcgagagga
     5641 cctggcgctt cacgctcgat cctttccgcc gcaagtgcct cctcgaaggc ctcgacgcgg
     5701 tctcgcttac gctcgagcat gaggacgaca tccgtgcaag agagaagcgc gagcccgcct
     5761 ggctcgtgcc tcatgtcgag gacctcaagt cctgtccggt ctgaagcgcc tgcgcctgaa
     5821 agcacggaat gcgcctgtct tcgccttcgg atcctcccgg tgggcgtgga ccgcgaagag
     5881 gccgcgcagc cactgcaggg cgggttcgtc ggcgacgcgt tcgtgccgga tgaggcgggt
     5941 gtcgaaggcg ccccggccgc ggttgctttc gggaccgaga tgaatgggaa tggcgacgat
     6001 gccgtcccgt tcctcgaaga gtcttgccgt ctgcatgggc atgactgccg tgaagtccgt
     6061 gggttgagaa gcgaggggac ggcgaggaaa tatgggatcg tgacggcggt ttcctgaagc
     6121 gcctctccga gcacggtcgt ctcgtccatg cagtacactt caagtcggtt ctgttcgcgg
     6181 ttcgtgaccg tgttcttgcg ccagtggcgg agcatgtcga gcgtcacctc gtccttttcg
     6241 cgggaggcct cgaccagcgg atgtctcttg cgaacgcaga gcgcgtagct gatcggatag
     6301 aaggagcatg tctatgactg cctgggtgaa tcgagcgtgt ctcagggtag accgcgcagt
     6361 cgagagacgc cgcgatatcc tgccctgatc aacgtcgatg ttcatgagac atgacgcgat
     6421 cgccgagagc cgaacatatc cggctcgaag atccacggaa acgatggcga tcgtcactcg
     6481 atagtcggca aagtcgagct tcattgcgcc gcccccctga tgcttgaaga gatccataat
     6541 caatcggtcg ggatcggcag actcgaactc gacgtgcgta ccgttgggca tgctcagacg
     6601 aaccgtgcgg gaaggagcga gttcgtcaag gtaagggaag tcaggaactg cctggagcgt
     6661 ctcggaggta aaagaggcaa cctgtacaac ttcggaagat gaactgccgg cgggattatg
     6721 aagttgcccg cttgctgcca gattctccag gagccgaaga gatgaataga acacgccaag
     6781 cggcggacgc ttgcgggagg aaatcagggt gggaagacac tggcgataga actctcgctt
     6841 gcttaacctg caagtcgtca aaagatcact caaagcagac acttctctta aaagccctgt
     6901 ttaatcgggg cttttttctt gtgccatgat gggtgtgtgt gatataaata atatacactc
     6961 atcaaaggag atcaaatggc agctcggaag gagttcccgg tcaaagttcg ccgtgtggca
     7021 gtaacgaaca aaaagaccgg cgtcaaatac atcgaggaac gtcgctatca atacgatccg
     7081 gcaaagggct acaacgtcct tctgtcgagc cgtcggacgg gtgaaaaaat cctggaaggc
     7141 gaaacggtga caacccgttg ccgccccaag aagaagccag ccgaagcggc tcagactgcc
     7201 gagctctctg ccaaacgaac ccgtgtaggg gctctggact tgattcgtca tgccggggca
     7261 gtggccggac tcgaaagttc ggtccgcagg gcgtatccca acggtggaac ctccgagaag
     7321 ctgctctctg tctctcagta cttggttgct acgggtgaaa ctgttcacaa cgtggaggcc
     7381 tggcaatgcg aacatgatct cccctacgaa gccggcctgt ccgaggacat ttgcgacgac
     7441 ctcttccacg agctcggcct ggatgaatcc ggttcgcagt ctctcttccg ggaactggcc
     7501 cgtacggcag gcaacgacga acagccggcc atcgcattcg attcaacctc gcattccgtc
     7561 tacggcaacg gcctgaaacc gtacgcgcgg caaggcttca acaaagacgg cgacggcttg
     7621 gacatctata agatcatcac cttctactcg cttgattccg gtctgcccgt tt
//
LOCUS       DBMDTI010000083         7668 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2560_length_7668_cov_2.315184, whole genome shotgun sequence.
ACCESSION   DBMDTI010000083 DBMDTI010000000
VERSION     DBMDTI010000083.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2560_length_7668_cov_2.315184"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="ACZPB6_05325"
     CDS             <1..184
                     /locus_tag="ACZPB6_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1183928.1"
                     /translation="VRINEKAEALTAEGKVMPCRWSAGEVTGVTHGTNRLGGNAYTDI
                     IVFGRIAGEAAAKAAK"
     gene            311..1054
                     /locus_tag="ACZPB6_05330"
     CDS             311..1054
                     /locus_tag="ACZPB6_05330"
                     /inference="COORDINATES: protein motif:HMM:NF020296.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR1183929.1"
                     /translation="MTPDEIHDRLSTLADPACKAFGDSLQPGVTDRLGVRMPLVRRVA
                     RDVMRTEDVRAFLNAMLAAGGFASQEALMVCVIVAGGAKALELEERLAFVDRLLPHMT
                     GWATCDLTGSAVKVFRENREELIGYVGEKLASDDPWTVRVAEVWLLEHYRDARWTQAA
                     LDLLGGGTSRALVLAASGDYYLSMSLAWCLSMLATADLEAVCSRIESWRAEGRLDDAT
                     LRRTVRKIRESLQFTKETKAAVSARFAAR"
     gene            1368..2150
                     /locus_tag="ACZPB6_05335"
     CDS             1368..2150
                     /locus_tag="ACZPB6_05335"
                     /inference="COORDINATES: protein motif:HMM:NF025446.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4261 domain-containing protein"
                     /protein_id="HIR1183930.1"
                     /translation="MTEETNASESEVQITPVEASVLLSGAEWDPKLLATTLAQEWEIT
                     VPEKDLPADASESLVFEAEGAVIAVKLFEEPLPAEMARMHAAMNPLWPQAGAMAQLHR
                     AHLAVAAIPKTASLVEAAQVLVKVVSSLCNQPSALAIDTGSTILGTDGYREGATVMKG
                     GELPLFNLVTFAIAKADETNFVAFTMGMSFLGHNELEISPTAEKPEVIQEFLYTVASY
                     TLKTGKGLRDGQILGLHPEKAQAVRYAESSMIPGLKTMQITL"
     gene            2178..2630
                     /locus_tag="ACZPB6_05340"
     CDS             2178..2630
                     /locus_tag="ACZPB6_05340"
                     /inference="COORDINATES: protein motif:HMM:NF038048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="HIR1183931.1"
                     /translation="MRLAEALAERKALDARLSSIEGRLRANARIQEGDAVSEDPERLF
                     ALLDSTAEELRTLMVRIARTNRETVVDGRPLADWIAERDVSVRRFNILSGVADRASER
                     IERRSADDVRVVPVIDVVARKHEADALAARIRGIDARIQAANWATELS"
     gene            2641..3096
                     /locus_tag="ACZPB6_05345"
     CDS             2641..3096
                     /locus_tag="ACZPB6_05345"
                     /inference="COORDINATES: protein motif:HMM:NF038048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="HIR1183932.1"
                     /translation="MKLAEALNARKELEARLTRLEERLHASARVQEGLEPDESPDALY
                     AMLEKTAAELVEIVSRISCTNVETVVEGRILADWIAERDVSWRRLRVLQSALRAAAPS
                     YDRHGGPGSVRSVTTFSTARRYAELDALALRINAIDARIQQANWETELL"
     gene            3868..4248
                     /locus_tag="ACZPB6_05350"
     CDS             3868..4248
                     /locus_tag="ACZPB6_05350"
                     /inference="COORDINATES: protein motif:HMM:NF015317.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HIR1183933.1"
                     /translation="MSIVFSDFAHTGRPWNRSSKNSSTARWILAPFFLGLSLALLALA
                     LKFFHEVWHVLPHVFELSESDLILSLLSMVDMGLVGGLIMMVMYSSYENFVSRLDLDE
                     KDSERLGWLGKMDAGSLKNKVALC"
     gene            complement(4427..5026)
                     /locus_tag="ACZPB6_05355"
     CDS             complement(4427..5026)
                     /locus_tag="ACZPB6_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012354.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1183934.1"
                     /translation="MKIRQIKAFLAIVSTGGVRPAAKKLCLTPSTVAKSIGQLESDLG
                     APLFERSALNLRLNAADPPSSSSSRAAFSQTAELSALVEEPLFEVDHGIVAAPGHPVL
                     APDADLKTVFAQSEWLTTVQDEGFLLEKLGGLGISPERVTLCDFFGIDALKGRNDALT
                     LSPLSVIEDSLYAGRPRRTSPCALSAFSPHRLLLSPKGG"
     gene            complement(5153..6358)
                     /locus_tag="ACZPB6_05360"
     CDS             complement(5153..6358)
                     /locus_tag="ACZPB6_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1183935.1"
                     /translation="MELIPRKDYLRQLRTFRDTQLIKVITGVRRCGKTTLMTLFINEL
                     KAGGIEDERIVRINFEDYDAIELTDPKALHAYLKSKLQPGKTVYFFLDEIQNVSDFQR
                     VIDSLYIRDNVDIYLTGSNGTMLSSDLATLLSGRYVEIKMLPMSFGEFVEGTHANGSL
                     SKSYRRYLEVGSFPYALSLPNAEAVRTYYEGVFSTILLKDLALRLKLSDTSLLLSICR
                     FLFENIGSLVAPKRIADYLNAQGRKCDVKTVDKYLNALMDCFVLYEARRFDIHGKAEL
                     ARLEKYYLVDLGLRRTVLGNKAMDVGHTLENVVYLELLRRGFEVYVGRVDQTEVDFVA
                     KSADGYQYIQVSATVRDPDTLARELRPLQNIKDHYPKLLLTLDDDPDMDHDGIRQCNA
                     LEWLLTRKP"
     gene            6624..6746
                     /locus_tag="ACZPB6_05365"
     CDS             6624..6746
                     /locus_tag="ACZPB6_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183936.1"
                     /translation="MAILRLHDTKRSLADALLEGADMSSRLSKEELLKLLSDQG"
     gene            complement(6883..>7668)
                     /locus_tag="ACZPB6_05370"
     CDS             complement(6883..>7668)
                     /locus_tag="ACZPB6_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183937.1"
                     /translation="TNPDNYETVEVVRHGKKHKSLKVKSGRLQWRFSKRYEKLRSELA
                     EMLRLCAATRKREHGEVCNRLLGHAGHIIVEDNSYKAFQKGRFGKAIGRHAPAALYAQ
                     LASKAESAGLLVEVVSPKKLKPSQHNLLTDTFVKHELWERRARLGEDDDDRRIDRDAA
                     ACLNLLYADLENQAYAPERIREAVPAGVTAWLDAGVAVIQAREGITEREFGRFRRRGI
                     PSLAVQGLRQQGFRGRSDGKSGATPKRKASTVSKPSHFSGEVV"
BASE COUNT         1469 a         2313 c         2345 g         1541 t
ORIGIN      
        1 cgttcgcatc aacgaaaagg ccgaggccct gacggccgaa ggcaaggtca tgccgtgtcg
       61 ctggtctgcg ggtgaagtga ccggcgtcac gcacggcacg aaccgtctcg gcggcaacgc
      121 ctacacggac atcatcgtct tcggccgcat cgcgggcgag gctgccgcca aggccgccaa
      181 gtaagaggac tcgatgacgg ggcgcatccg cgttctgcgt ccggatccgc catgaagccg
      241 ctcccggcgt tttcgccggg gcggcttttt caacagtctt ccatttgatt tcgaacgaaa
      301 tcccgaaacc atgacgcccg acgaaatcca tgaccgcctc tcaactctgg ccgatcccgc
      361 ctgcaaggcc ttcggcgaca gcctccagcc cggcgtcacc gaccgcctcg gcgtgcgcat
      421 gccgctcgtg cgaagagtgg cccgagacgt catgcgcacg gaggacgtgc gcgcctttct
      481 caacgccatg ctcgctgcgg gcggcttcgc ctcccaggag gcgctgatgg tctgcgtgat
      541 cgtggcgggc ggcgcgaagg cgctcgagct cgaggagcgg ctcgccttcg tcgacaggct
      601 cctgccgcac atgacgggct gggcgacctg cgacctgacg ggatcggctg tcaaggtctt
      661 cagggagaac agggaggagc tcatcggcta cgtcggcgaa aagctcgcct cggacgatcc
      721 ctggaccgtg cgcgtggcgg aggtctggct ccttgagcac taccgcgacg cccgatggac
      781 gcaggcggcg ctcgaccttc tgggaggcgg gacgagcagg gcgctcgtgc ttgccgcctc
      841 gggtgactac tatctttcga tgtcgcttgc ctggtgcctc tccatgctcg cgacggcgga
      901 ccttgaggcc gtctgctcgc gcatcgagtc ttggcgcgca gaaggccgcc ttgacgacgc
      961 cacgctgcgc aggaccgtga ggaagatccg cgagtcgttg cagttcacga aggagacgaa
     1021 ggcggcggtg tcggcaaggt tcgccgcaag gtgagaggcg acgggaccgc ctcccctgag
     1081 gcggccgtct tcggaacgtc cgtcttcaaa ttgcaagttc attttcaaca ggcatgtccg
     1141 gctcgttccc gtgcagttct aggtcctcgg cagcccggag cggcgagtgt ctgcagacca
     1201 cggcgtacgg gatgcggtcg tcctcgcccg gcatcacggt cctgaggaac gcgggaggga
     1261 aagcgcagcg gaccggcggg ctataatgcc tgatccatgc ggtccggcgt ccgaggagag
     1321 cgctgctcga agccgcacat ccctttgaga caaacacgaa gttcatcatg accgaagaaa
     1381 ccaacgcttc cgaatccgaa gtccagatca cgcccgttga ggcctccgtc ctgctgtccg
     1441 gtgccgaatg ggatccgaag cttctcgcca cgacccttgc ccaggaatgg gagatcacgg
     1501 tgcccgagaa ggacttgccc gccgacgcct ccgagtccct cgtgttcgag gccgagggcg
     1561 ccgtcatcgc cgtgaagctc ttcgaagaac cgcttcccgc cgagatggcc cgcatgcacg
     1621 ccgccatgaa cccgctctgg ccgcaggcgg gcgccatggc ccaactgcac cgcgcccacc
     1681 tcgccgttgc tgcgattccg aagaccgcct cccttgtcga ggccgcccag gtgctcgtca
     1741 aggtggtttc ctcgctctgc aaccagccct cggcccttgc catcgacacg ggctccacga
     1801 ttctcggcac ggatggctac cgtgagggcg ccaccgtcat gaagggcggc gaacttccgc
     1861 tctttaatct cgtcaccttc gcgatcgcga aggccgacga gaccaacttc gtcgccttca
     1921 ccatgggcat gagcttcctc ggtcacaacg agctcgagat cagtccgacc gccgaaaagc
     1981 ccgaggtgat ccaggaattc ctctacacgg ttgcttccta cacgctcaag acgggcaagg
     2041 gcctgcgcga cggtcagatc ctcggtctcc atcccgagaa ggcccaggcg gtccgctacg
     2101 ccgaaagcag catgatcccc ggtctcaaga ccatgcagat cacgctctga gccgcgccgc
     2161 aggttgggag acatagtatg aggcttgcag aagcgctcgc tgagcgcaag gcgctagacg
     2221 cgcggctctc ctcgatcgag ggccgcctca gggccaacgc ccgcattcag gagggcgacg
     2281 ccgtgagcga ggatcccgaa aggctcttcg cgctcctcga ctcaaccgcc gaggagctcc
     2341 gcaccctgat ggtgcgcatc gcccgcacga acagggagac tgtggtcgac ggtcgtccgc
     2401 ttgcggactg gatcgccgag cgcgacgtct ccgtgcgccg cttcaacatc ctgtcgggcg
     2461 tcgccgacag ggcgtccgag cgcatcgagc gccgcagcgc ggatgatgtc cgcgtcgtgc
     2521 ccgtcatcga cgttgtcgcc cgcaagcacg aggccgacgc gctcgccgcc cgcatccgcg
     2581 gcatcgacgc ccgcattcag gccgccaact gggcgaccga gctcagctga ggagatcttg
     2641 atgaagcttg ccgaagcact caacgcccgc aaggagctcg aggcgcgcct cacgcgtctt
     2701 gaggagaggc tccacgcctc ggcccgcgtc caggaaggac tcgagcccga cgagtcgccc
     2761 gacgcgctct acgctatgct cgagaagacc gccgcagagc tcgtcgagat cgtctcgagg
     2821 atctcctgca ccaacgtcga aaccgtcgtc gagggccgca tcctcgccga ctggatcgcc
     2881 gaacgcgacg tctcctggag acgcctgcgc gtgctccaga gcgccctcag ggccgccgcc
     2941 ccgtcctacg accgccacgg cggtcccggc tcggtcaggt cggtgacgac cttcagcacc
     3001 gcccgccgct atgcagagct cgacgcgctt gcgcttcgca tcaacgcgat cgacgcccgc
     3061 atccagcagg cgaactggga gaccgagctg ctctgacgat catgccgtcc ggcgaagcct
     3121 cgtgaaggcg gagccgccgc tgtcaggccg gatcggccgg aagccgccgg tcagaactgc
     3181 acttttacga ttcacttcca cgctcgtact ttcacggttc agcacgaagc cttcttacca
     3241 ttggctcgat tcacggaatt cgttcggacg gcacccatcc aaagcctcga ggcgctgcat
     3301 catgtgatgc ggcgcctttt tcaaggcgtc cgaaggcggg cggacaattc ttgttgcact
     3361 tttcactgta tgcccggttc tggaaatgcg acctcctgac gggaactgaa ttgatcgagc
     3421 actgaacgag tcgttttgta ggaggcgggg gcgggaaggc aagatgaggc gaaatgcgtg
     3481 cggtttcgtc cgttcttttt gtgcgtacgg cagtgaatct cgtgagctcc aacgggtttc
     3541 gcgtgtgcga tgaattcttg gtttttcttg atgcagagtg cttgagcgtg agcggatgtg
     3601 tgaccttatg ggcgtctcct gcccttcttg cattgtcggc tcccgcttcg tgtgaacgat
     3661 ctgcagaatc gtttgcttga cgaggcgttt gagttcgaac gcgctctgca atgcgatgct
     3721 cgggcctgtt cccgggcgtc tgcagcaggc agcatgcgta cctcaggaaa ccgctgaaga
     3781 aaaagcggga ataccgtcat ccgggatgga cgcacgaaga agcctgcatg ttgtagaatc
     3841 tgtggccctc gtgctatcgg cttgtgtgtg tcgatagttt tttcagactt cgctcatacg
     3901 ggacgcccat ggaatcgttc ttcgaaaaac tcttctacag cgcgctggat tcttgcgccg
     3961 ttctttctcg gcctgtcgct tgcgcttctg gcgctggcgc tcaagttctt ccatgaggtc
     4021 tggcacgtgc tgccgcacgt cttcgagctc tcggagtccg acctcatcct ctcgcttctt
     4081 tcgatggtcg acatgggcct ggtgggcggc ctgatcatga tggtcatgta ctcgtcctac
     4141 gagaacttcg tttcgaggct tgaccttgac gagaaggatt cggagcgtct cggctggctc
     4201 ggcaagatgg acgcgggcag cctcaagaac aaggtggcct tatgttagtt ggaatttatt
     4261 cccgtttctt ttgaaaatgg tttcaaaggt ggaattgatt ccaagaaaca taagtgagcg
     4321 tgttggtctc caacgcgctc gaatgaagcg gcgcggcttg gcggccatcc attgcgcgaa
     4381 ggccaggcga agcgcgtggc gcatgaagtc ggcgggcggc gagggctcaa ccgcctttcg
     4441 gtgaaagaag gagacggtga ggggagaaag cggaaagagc gcagggtgaa gtgcgtcgag
     4501 ggcggcctgc gtagaggctg tcctcaatga cgctcaaggg agagagggtc aaagcgtcat
     4561 tgcgtccctt gagcgcatcg atgccgaaga agtcgcagag cgtcactcgc tcgggtgaaa
     4621 tgccgagacc gccgagcttt tcgagaagaa agccttcgtc ctgcacggtg gtgagccact
     4681 cgctttgagc gaacaccgtt ttgagatccg catcgggcgc gagcaccgga tggccggggg
     4741 ccgcgacgat gccgtggtcg acctcaaaga gcggctcctc gacgagcgcg gaaagttctg
     4801 cagtctgaga aaaagccgct cgtgaagacg agcttgacgg cgggtcggcg gcattgagcc
     4861 tcaggttcag agcgctgcgc tcaaagagcg gtgcgccgag atccgactcg agctggccga
     4921 tggatttggc gaccgtcgac ggcgtgagac agagcttctt ggccgccggg cgcacgccgc
     4981 ccgtggacac gatggccaga aatgccttga tttgacgaat tttcatgaag aacctcaggg
     5041 cggctgcccg aggcgatgga tcgaaggatg acggtacctg catccattgt ctcgaatatc
     5101 gcgcaatggg agccatgcag gccaggtgcc gtcgccggcg gcgagaagag cctcacggct
     5161 ttcgcgtcag caaccattcc aacgcattgc actgccggat gccgtcgtga tccatgtccg
     5221 ggtcgtcgtc gagggtgagc aggagtttcg gataatggtc cttgatattc tgcaagggcc
     5281 tcaattcccg tgcaagggtg tcgggatccc gcacggttgc cgacacttgg atgtattggt
     5341 agccgtcagc cgatttggca acgaagtcca cttccgtctg atcgacgcgc ccgacgtaga
     5401 cttcaaatcc gcggcgcagc aattcgagat agaccacatt ttccagcgtg tgcccaacat
     5461 ccatcgcctt gttgccgagc accgttcggc gaagcccaag gtcaaccaga tagtatttct
     5521 ccaaacgggc cagctcagcc ttaccgtgaa tgtcgaagcg tctcgcttca tagagtacaa
     5581 agcagtccat caacgcattg aggtacttgt cgacggtttt gacgtcgcac tttcgaccct
     5641 gtgcattcag gtagtccgca attcgtttgg gtgcaacaag gctcccaata ttctcaaaga
     5701 gaaagcgaca aatgctcaga aggagcgacg tgtccgagag cttcagccgc agcgccaaat
     5761 ccttgagcag gattgtgctg aagactccct cataataagt gcgcaccgcc tcggcattgg
     5821 gaagagagag cgcataaggg aaggagccga cttccagata tcggcgatag cttttactga
     5881 gcgaaccatt ggcgtgagtg ccttcaacaa actcgccgaa ggacatcggg agcatcttga
     5941 tttcgacata cctcccgctc aacagtgtgg cgaggtctga ggacagcatc gtgccgtttg
     6001 atcccgtcaa atagatgtcg acattgtcgc gaatgtagag gctgtcgatg acgcgctgga
     6061 aatccgaaac gttttggatt tcgtcgagaa agaaatagac ggtctttccg ggctgtagct
     6121 tcgacttcag ataggcatgc agggctttcg ggtcggtcag ctcaatggcg tcatagtctt
     6181 cgaagttgat gcgaacgatc cgctcgtctt caatgccacc tgccttcagc tcattgatga
     6241 agagcgtcat caaggtggtc tttccgcatc gacggacgcc cgtgatgact ttgatgagct
     6301 gggtgtctcg aaatgtgcgc agctggcgca gatagtcttt tcttggaatc agttccatct
     6361 tagacgtgtt ttgcaaagga gttggactaa attccaatat aacatgggtc tgcatgggtg
     6421 gttggctttg gagccgttca gttgcttctg tttgttccaa gtcggcattc aggtggtgtt
     6481 cgggtctggg ttgcgcaagc ttggaaacga agcggccttc cgtctgcaga gctaacgtgc
     6541 gaacgaaagg ccgcggagga gtcttcagat gtcggcgcaa gccttatgcg gcggcgagtt
     6601 cgcggccgac gagccaaccc caggtggcga tccttcgcct gcacgatacg aagcgctcgc
     6661 tcgccgatgc gcttcttgag ggcgccgaca tgtcgagccg tctctcgaag gaggagctcc
     6721 tcaagctcct ttccgaccaa ggctgacaac ctcagacaca tggcgatgga cgccccgccg
     6781 aacgacatgc gttcggcggg cgcagacgaa cacgaacgga gaaatcgatg ggcgatgcga
     6841 cctcgacgac gcttgacctc tcagacctga cggacgttga gcctaaacaa cctctccact
     6901 gaagtgggag ggtttcgata cagtcgaggc ttttcgcttc ggggttgccc cagatttgcc
     6961 gtcactccga ccacgaaatc cttgctgacg cagcccttgt acggcaaggg acggaatccc
     7021 ccgccgtcgg aacctcccga actcgcgctc ggtgattcct tctcgggctt gaatgactgc
     7081 gacaccggcg tcaagccatg ctgttactcc ggctggaacg gcttcacgga tccgttccgg
     7141 ggcgtaggct tgattttcaa gatctgcgta caacaggttc agacaagccg ccgcatcgcg
     7201 gtcgatccgt cgatcgtcgt catcttcacc cagtcgcgcg cggcgctccc aaagttcgtg
     7261 cttgacaaac gtgtccgtca gcagattgtg ctgagagggc ttgagtttct ttggcgatac
     7321 gacttccacc aacaaaccgg cgctttcagc cttgcttgcg agttgagcgt ataacgctgc
     7381 cggcgcatgt cgcccgatgg ccttgccgaa gcgtcccttc tggaacgcct tatagctgtt
     7441 gtcctccaca atgatgtgac ctgcatggcc gagaagccgg ttgcagactt caccgtgctc
     7501 gcgttttcgc gtggcggcgc aaagacgaag catctccgca agttccgacc ggagcttttc
     7561 gtagcgtttc gagaaacgcc actgaagcct tccggacttc accttcagag acttgtgctt
     7621 cttgccgtga cgaaccacct caaccgtttc gtagttgtcg ggattcgt
//
LOCUS       DBMDTI010000084         7655 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2564_length_7655_cov_1.917792, whole genome shotgun sequence.
ACCESSION   DBMDTI010000084 DBMDTI010000000
VERSION     DBMDTI010000084.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2564_length_7655_cov_1.917792"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="ACZPB6_05375"
     CDS             complement(<1..1569)
                     /locus_tag="ACZPB6_05375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1183938.1"
                     /translation="MNRFLTTTLSACLLLECSAAFAEGAPLRMGISGFALPEHRTEIV
                     RATEKVIRPMFGEDGLTVRDYTVSELEEAVRRGEVDIILSSAGLARRVSPYGVRPLVT
                     VTAPGLENPNSNEGSLLVVRADSSVRTLDDLKGASVSANLPWGFSGWQIALGEVAAAG
                     HDPDRFFGEKVFVGRSAGMTEIAQNVRSGRTVAGVLRLCAYESMAETDPTLIDGLRIV
                     SERREPGVACRVSTQLYPAQTLSVTPRVDAQTARELMLRLLQMAPTADGRYWSVASDF
                     HHVDELLKTLKIGPYRYLREWSLRRFLEVAWPWLMLACFLVAGLYWHSRRTERLLALR
                     GELLGRMFAREAEQAERLTFLQRSNTVNQISSMVAHDLRQPLAALGFYADGLRMMVDL
                     GRCDEDKLQRFSRGITKEVARASGILDSVRAYARNSRHPRRALVVDNLIEEAIGIFQA
                     GRPNKIHVAVKIAQPGLSVKGNELELTLVFVNLLKNAREAVEKEAVRKVCIEVRRIPD
                     DGATVEVRVLDNGPR"
     gene            2244..2366
                     /locus_tag="ACZPB6_05380"
     CDS             2244..2366
                     /locus_tag="ACZPB6_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183939.1"
                     /translation="MSLGKQYLDLAVSLRRAIHKRPEPGWCEFETTYKIVRPRL"
     gene            complement(2375..2548)
                     /locus_tag="ACZPB6_05385"
     CDS             complement(2375..2548)
                     /locus_tag="ACZPB6_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183940.1"
                     /translation="MVFAFAACVEKTDGAGVAARGVDERLRHAVGERAFDGGLHLGFV
                     SAHDGFGRDGVMG"
     gene            2580..3110
                     /locus_tag="ACZPB6_05390"
     CDS             2580..3110
                     /locus_tag="ACZPB6_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1183941.1"
                     /translation="MQRCDIVMGYAPSHDDPNLVKRYCSTIPYIARASRAYVKEFGSP
                     ATPADCFRHRGILINSVVRTSVKMLSKNGVTMPLPWKSTMVLSNPIAAARALSCGAGI
                     VPDMSLYHAYQHLESGEWQIVMPGWQRPSVDAFLYTTEETYRKRRIRTLIDWLSEHNQ
                     KIDKHLRTDYPKLFLR"
     gene            3204..3668
                     /locus_tag="ACZPB6_05395"
     CDS             3204..3668
                     /locus_tag="ACZPB6_05395"
                     /inference="COORDINATES: protein motif:HMM:NF025569.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HIR1183942.1"
                     /translation="MCPVCGKFEFTELQETDLLFRDEMQCSICGWKYDQHQHEDHDLK
                     NGLNELSLNEYQAWYKQKIKENPDFDFQDENYQAAAHMCPVCHHYQFEDENSFDVCPF
                     CGWCDDALMENEPDKWADNANDLCLNEFRERYQKFCLANPKYRFEKNGFRNS"
     gene            complement(3858..5684)
                     /locus_tag="ACZPB6_05400"
     CDS             complement(3858..5684)
                     /locus_tag="ACZPB6_05400"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008541534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HIR1183943.1"
                     /translation="MFKKSLIAVASLLAAVGTAQACSTLVIGKAVSETGNIIVAHNED
                     NGGRLFNMQHYVPPAKHKKGEMLKFEKFAAEIPQVEETLGFYWTQTFVPDGASFADGF
                     FNDAGVVVATNWCGEIFDADRMEVKDGGIGYGIRRLVAERAHSAREGVEIATKLLAEF
                     GYFHDGRTYTIADANEAWQLAIHQGNSWVARKIHDNEVVYIPNNFLMDKVDLNDKETW
                     KIEPKQVERAIKDGRHDAKNPIFNWRKVVAQESVRHERWNANRNVLAWKFLTGVEYND
                     PEKFPYSAVIDRKLGVKDAMALLRLHEDYIGQDQELYHSKSEGICRTTSHDSIVYNLT
                     ADPTLTEAWKTLGRPCQSVFVPLYPLAGPAKGTAFTDPATATKEHFAGTPAMFDYRAD
                     FTPHSVFSASNNAIDYLRGDELAKRTQLIQAEEEKFMKDRAAVTKKAAALKGEERTKF
                     LHDYNQKSYDHVLKIMQAENARLMPNKVKILADVVKADGDGTVDFALLGAKGHEVLSA
                     NMEATRAGMSANQLNSTRQWKNFAPAEKMVYKDVNNDGITDVVFTFKAKDVTKGAVAG
                     AAMDLWLYTQINGHRVTGFDVVPVENDKVKMHKGARDGKGLL"
     gene            complement(6146..6778)
                     /locus_tag="ACZPB6_05405"
     CDS             complement(6146..6778)
                     /locus_tag="ACZPB6_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1183944.1"
                     /translation="MSRYGIIFNVDRCIGCEACFVACKEENKVAPGIQWNKIRRIENV
                     GKRVINYYRVSCQHCEDPACMKVCPAKAISKGPHGEVLVDHKKCIGCRMCLAACPYGA
                     PLFNEDGRTGYFGDKEPLLKPEPKAHQVRVPGKAEHCTLCTHRLAEGRLPACVENCST
                     KALTLVDYESKDPEVQALIKRSICLSEEAGTQPKVRYICSNMDFKSVKLK"
     gene            complement(6789..>7655)
                     /locus_tag="ACZPB6_05410"
     CDS             complement(6789..>7655)
                     /locus_tag="ACZPB6_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR1183945.1"
                     /translation="AKAISKLEFAFATDYFYRKESHRDLDLILPAAMNFERYAPFGMY
                     GAKYAPRTPVKPLGEAKEDWRIALEIGCLVDKPEDFFDGDPVKACNAVLKVWEGNYEK
                     AVAALPAVSTLKCEKNEPYKYVSGKLRPDGKPGFNTPTGKLELFSTITSKYGLEGLPV
                     YKEMMKPEGDFNLRLINGTRKPYITHSKTRSDAPYLLELEPVSTIHMNPADAKKRGLV
                     TGDFVEMRSKFGGPVRARIDVSIIVPEGTIDSQYGWRGDQNTQNLIPRQWDPISGYAP
                     YFEVNVSVAKVK"
BASE COUNT         1501 a         2326 c         2274 g         1554 t
ORIGIN      
        1 acgcggacca ttgtcgagca ctctgacctc caccgttgcg ccgtcgtccg gaatgcggcg
       61 aacctcaatg cagaccttgc gcaccgcctc cttctcaacg gcctcgcgtg cattcttaag
      121 gagattgacg aacaccagcg tcagctcgag ttcgttgccc ttcacgctca ggccgggctg
      181 cgcgatcttc acggccacgt gaatcttgtt tggacgaccc gcttgaaaga tcccgatcgc
      241 ctcttcaatc agattgtcga cgaccagtgc cctgcgggga tggcggctgt tccgggcgta
      301 ggcgcggacg ctgtccagaa tgccgctagc tctcgccacc tccttcgtga tgcctcgaga
      361 aaacctctga agcttgtcct cgtcgcaacg cccgaggtcc accatcatgc gcagaccgtc
      421 ggcataaaac cccagagccg ccagaggctg ccgcagatca tgagccacca tgctcgaaat
      481 ctgattgacg gtgttgctgc gctgcaaaaa ggtgagcctt tcggcctgct cggcctcacg
      541 ggcgaacatg cgtcccaaaa gctccccgcg caaagccaga agcctttcgg ttctccggct
      601 gtgccaatac aggcccgcga ccagaaaaca cgccagcatc agccagggcc aggcgacttc
      661 cagaaagcgc ctgaggctcc actcccgaag ataccggtag ggcccgatct tcaaagtctt
      721 cagcagttcg tccacatgat ggaagtcgct ggcgacggac cagtatctgc cgtcggccgt
      781 cggagccatc tgaaggagcc tcagcatcag ctcgcgagcg gtttgagcgt cgacgcgagg
      841 cgtgaccgag agcgtctgcg cgggatagag ctgcgtggac accctgcagg caacgcccgg
      901 ctcccttcgc tcgctgacga tccgcaggcc gtcgatcagc gtcggatcag tctcggccat
      961 cgactcgtag gcgcacaaac gcaggacgcc ggcgacggtc cggcccgaac ggacgttttg
     1021 agcgatctcc gtcatgcctg cagaacgtcc gacaaacacc ttttcgccga aaaatcggtc
     1081 cggatcatgc cctgcggcgg ccacttctcc aagcgcaatc tgccatcccg aaaagcccca
     1141 gggcaggttg gcgctcaccg acgcgccctt cagatcgtcg agcgtccgaa ctgaactgtc
     1201 ggcgcgaacc acaagcaacg acccctcatt ggaattcggg ttctcgagtc ccggcgccgt
     1261 cacggtcaca agaggtctca cgccgtacgg cgacacgcgg cgcgcgagtc cagcgctgct
     1321 gagaatgatg tccacctcgc cccgcctgac ggcctcctcg agctcgctca ccgtatagtc
     1381 gcgcacagtc aggccgtcct caccgaacat cggacgaatg accttctcgg tcgccctcac
     1441 gatctccgtg cgatgctcgg gaagtgcgaa tcccgaaatg cccatgcgaa ggggcgcccc
     1501 ctcggcaaag gcagccgaac actcaagcag cagacacgcc gacagcgtcg tcgtcaggaa
     1561 acgattcatc gagggaaagg cagggaagaa aaatgaggac acgttcgtcc tcattgcgaa
     1621 tgattgaaaa acgggacccg cagcacagcc cggagcgcgg tctttttttg tgccaaaaca
     1681 tctgcgttga tccggagttt tgaaaatcgc ggcagggttt tcgcttccac ctgcgcgatg
     1741 caggcgcgtg agcagacgaa tgaacaggcg ctcagaatca tccgtttcct cttcaccggc
     1801 gttgggcttc ggatcccgtc gacagcccct tttcatgtgc aggagaggct tatgaacatt
     1861 ttaaagcaaa gccgtcgcga accgtcagag ccctaaacgc ccgttcccca tttccgctca
     1921 ccctcatctg ggaattaccc gcagaacttg catttcaagc acccgcagtt tgtcgttttt
     1981 gaaacccgct ttctcgatcg aaaaacgatt tcccctctcc cgaagtcaca aggcgtcatc
     2041 aatttgccgt tttggcaaat tcccattgct caatttgtct tttctgccgc catgaggttt
     2101 tcgataattg tgaccgcgcc gcatgccgtc cgccgaagag gaccaagtcc ctggaacgga
     2161 cgcccgcatg gaatggatcc ggcccctgcg catggtccgg tccaccgcgc ccagtccccc
     2221 gccacaatca aaaggaaact tccatgtccc tcggaaagca gtatctcgac ctcgccgtat
     2281 cgcttcgacg cgccattcac aagcgtccgg agccgggctg gtgcgaattc gaaaccacat
     2341 acaaaatcgt caggcctcgg ctttgacgtg aagttcagcc catgacgccg tcgcgcccga
     2401 agccgtcatg ggctgaaacg aagccgaggt gcaggccgcc gtcgaacgcg cgctcgccca
     2461 cggcgtgccg aagtcgttca tcgacgcctc gggcggctac accggcgccg tcggtctttt
     2521 cgacacaggc cgcgaaggca aagaccacga cgctcgaata tccgctctac gccaccggtg
     2581 tgcaacgctg cgacatcgtc atgggctacg cgccgtcgca cgatgatccg aacctcgtca
     2641 agcgctactg cagcacgatt ccctacatcg cccgcgcctc cagagcctac gtgaaggaat
     2701 tcggctcgcc cgcgacgccc gccgactgct tccgccaccg tggcatcctc atcaactccg
     2761 tcgtgcgcac ctccgtcaag atgctttcca aaaacggcgt cacgatgccg ctcccgtgga
     2821 agtcgacgat ggtgctgagc aacccgatcg ccgccgcccg cgccctgtcc tgcggggcgg
     2881 gcatcgtccc cgacatgtcg ctctaccacg cataccagca tctcgaaagc ggcgaatggc
     2941 agatcgtcat gccgggctgg cagcgcccga gcgtcgacgc cttcctctac accaccgagg
     3001 aaacctaccg aaagcgcagg atccgcacgc tcatcgactg gctctcggaa cacaaccaga
     3061 agatcgacaa gcaccttcgc accgactacc cgaagctctt cctgcgctga cggcgccccc
     3121 ggagcacgaa gctcccgatg ctctgagtaa ccctgaaaaa acagccaaaa gaaatgattg
     3181 acaaaagttc ctatcaccca atgatgtgtc ccgtatgcgg caagttcgaa ttcaccgaac
     3241 tacaagaaac agatttgctt ttcagagacg aaatgcagtg ctcgatctgc ggctggaaat
     3301 atgatcaaca ccagcacgaa gatcacgatc taaaaaatgg gcttaatgaa ctttccttga
     3361 acgaatatca ggcatggtac aagcaaaaaa tcaaggaaaa tcccgacttc gacttccagg
     3421 atgagaatta ccaggctgcc gctcatatgt gcccggtctg ccatcattat caattcgaag
     3481 atgaaaacag ctttgacgtc tgtccgtttt gtggttggtg tgatgacgcc ctaatggaaa
     3541 acgaaccgga caaatgggca gacaacgcaa acgacctttg tctaaatgaa ttcagggaac
     3601 gctatcaaaa gttctgcctt gcaaatccaa agtatcgttt cgagaaaaac ggctttagga
     3661 attcttgaat cggacagggg tgagcttaga atctgcagac taaatgctga aggaatgcgg
     3721 cgagtcggct gaacgcgcaa atttgccgtt tgcggaatcc gatgccgcaa caatgctcaa
     3781 gggcgcgcga ttcgatgaac cgcgcgccct cttttcaggt caggaacagg tcccgctgcg
     3841 tggacccgtc gtcgtgatta aaggaggccc ttgccgtcgc gggcgccctt gtgcatcttg
     3901 accttgtcgt tttcgacagg caccacgtcg aagcccgtca cgcgatggcc gttgatctgg
     3961 gtgtagagcc aaaggtccat ggcggcgccg gcaacggcgc ccttcgtgac gtccttggcc
     4021 ttgaacgtga agacgacgtc ggtgatgccg tcgttgttga cgtccttgta gaccatcttt
     4081 tcagccggag cgaagttctt ccactggcgg gtggagttga gctggttggc gctcatgccg
     4141 gcgcgggtcg cctccatgtt ggcgctcagc acttcgtggc ccttggcgcc gagcagcgcg
     4201 aagtcgaccg tgccgtcgcc gtcggccttg acgacgtcag cgaggatctt gaccttgttg
     4261 ggcatcaggc gggcgttttc ggcctgcatg atcttgagga catggtcata ggacttctgg
     4321 ttgtagtcgt gcaggaactt cgtgcgttcc tcacccttga gggcggcggc cttcttcgtc
     4381 acggcggcgc ggtccttcat gaacttttct tcctcagcct ggatgagctg cgtgcgcttg
     4441 gcgagttcgt cgccgcgcag gtagtcgatg gcgttgttgg aggcgctgaa gacgctgtgc
     4501 ggcgtgaagt cggcgcggta gtcgaacatg gcgggagtgc ccgcgaagtg ttccttcgtg
     4561 gccgtggccg gatccgtgaa ggcggtaccc ttggcgggac cggcgagcgg atagagcggc
     4621 acgaagacgg actggcacgg acggccgagc gtcttccagg cttcggtgag cgtcggatcg
     4681 gccgtcaggt tgtagacgat cgagtcatgg gacgtcgtgc ggcagatgcc ttcggacttg
     4741 gagtgataga gttcctggtc ctgaccgatg tagtcctcgt gcaggcgcag gagggccatg
     4801 gcgtccttga cgccgagctt gcggtcgatg acggcgctgt acgggaactt ttcaggatcg
     4861 ttgtattcga cgcccgtgag gaacttccag gcgaggacgt tgcggttggc gttccagcgt
     4921 tcgtggcgaa cggattcctg agccacgacc ttgcgccagt tgaagatcgg gttcttggcg
     4981 tcatggcggc cgtccttgat ggcgcgctcg acctgcttgg gctcgatctt ccaggtttcc
     5041 ttgtcgttca ggtcaacctt gtccatgagg aagttgttcg ggatgtagac gacttcgttg
     5101 tcgtgaatct tgcgggcgac ccagctgttg ccctgatgga tggcgagctg ccaggcttcg
     5161 ttcgcatcgg cgatcgtgta ggtgcggccg tcgtggaagt agccgaattc ggcgaggagc
     5221 ttcgtggcga tttcaacgcc ttcacgggcg gaatgggcgc gttcggcgac gaggcggcgg
     5281 atgccgtagc cgatgccgcc gtccttgact tccatgcggt cggcgtcgaa gatctcgccg
     5341 caccagttcg tggcgaccac gacgccggcg tcgttgaaga agccgtcggc gaagctcgcg
     5401 ccgtcaggaa cgaaggtctg cgtccagtag aagcccagcg tctcctcgac ctgcgggatt
     5461 tcggccgcga acttctcgaa cttcagcatt tcacccttct tgtgcttggc gggcggaacg
     5521 tagtgctgca tgttgaagag acgaccgccg ttgtcttcgt tgtgggcgac gatgatgttg
     5581 cccgtttcgg aaacggcctt gccgatcacg agcgtgctgc aggcctgggc cgtgccgacg
     5641 gcggcaagca gcgaggcaac ggcgatcaga gacttcttga acattttcga actcctttgt
     5701 catgagaacg gcggacccgt ggtcgctgac ccgccgaaat tggtcgccac gaagtctata
     5761 gacccgcaaa cgcaggcacc acggtgtttc cgagggattg acaacggcaa gaaagtcaat
     5821 tatgggcaac atatgcggca tgaccgcacg aagcgattcc tgagaacagc tcgagctctt
     5881 cgagcaaccc gatttcagga tcaccggctt cgtcggcagc tccttcgccg tcgtgccctc
     5941 gggcaggttc tgcgccgtga cggactacat cgtctacggc cgcatcgcgg gccgcaatgc
     6001 cgccaaggag acgccctaga ggtgagtcaa tcctgaagct cctgagtcct ccgtcctgct
     6061 tcagtcccac ataaacagaa agccgtgcag gtcgcgagac cgcacggctt tcatggtcat
     6121 cagaacagga cgtccgtcag gaaccctact tcagcttgac ggacttgaag tccatgttcg
     6181 agcagatgta gcggaccttg ggctgcgttc ccgcctcttc ggagaggcag atggagcgct
     6241 tgatgagcgc ctggacttcg ggatccttcg actcgtagtc gacgagcgtc agggccttgg
     6301 tcgagcagtt ttcgacgcag gcgggcagac gcccttcggc gagacgatgg gtgcagagcg
     6361 tgcagtgctc ggccttgccg gggacgcgca cctgatgggc cttgggctcg ggcttgagga
     6421 gcggctcctt gtcgccgaag tatccggtcc tgccgtcctc gttgaagagc ggcgcaccgt
     6481 acgggcaggc ggccaggcac atcctgcagc cgatgcactt cttgtggtcg acgagcactt
     6541 caccgtgcgg ccccttggag atcgcctttg cggggcagac cttcatgcat gcgggatcct
     6601 cgcagtgctg gcacgagacg cgatagtagt tgatgacgcg ctttccgaca ttctcgatgc
     6661 ggcggatctt gttccactga atgccgggag cgaccttgtt ttcttccttg caggccacaa
     6721 agcaggcttc gcagccgatg cagcggtcta cgttgaaaat gatgccgtag cgactcattg
     6781 cagcctcctt acttgacctt ggcaacgctc acgttgactt cgaaatacgg cgcgtagccc
     6841 gaaatcggat cccactggcg cgggatgaga ttctgggtgt tctggtcgcc gcgccagccg
     6901 tactgcgagt cgatcgtgcc ttcgggcacg atgatcgaga cgtcgatcct tgcgcggacg
     6961 ggaccgccga acttgctgcg catttcgacg aagtcgcccg tgacgaggcc gcgcttcttc
     7021 gcgtcggcag gattcatgtg aatcgtgctg acgggctcga gctcaagcag gtacggcgca
     7081 tcggagcggg tcttcgaatg cgtgatgtag ggcttgcgcg tgccgttgat gagtcgcagg
     7141 ttgaagtcgc cctcgggctt catcatctcc ttgtagacgg gaagaccctc gagaccgtac
     7201 ttcgacgtga tcgtggagaa gagctcgagc ttgcccgtcg gcgtgttgaa gccgggcttg
     7261 ccgtccggac ggagcttgcc ggagacgtac ttgtagggtt cgttcttctc gcacttgagc
     7321 gtcgagacgg caggcagggc ggcgacggcc ttttcgtagt tgccttccca gaccttgagc
     7381 accgcgttgc aggccttgac cgggtcgccg tcgaagaagt cctcgggctt gtcgacgagg
     7441 cagccgattt caagcgcaat gcgccagtcc tccttcgctt cgccgagcgg cttgacaggc
     7501 gtcctcggcg catacttcgc gccgtacatg ccgaacggcg catagcgctc gaagttcatg
     7561 gcggcgggca ggatgaggtc gaggtccctg tggctttcct tgcggtaaaa gtagtcggtc
     7621 gcgaacgcga actcaagctt cgagatcgcc tttgc
//
LOCUS       DBMDTI010000085         7629 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2578_length_7629_cov_2.377524, whole genome shotgun sequence.
ACCESSION   DBMDTI010000085 DBMDTI010000000
VERSION     DBMDTI010000085.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2578_length_7629_cov_2.377524"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="ACZPB6_05415"
     CDS             complement(<1..459)
                     /locus_tag="ACZPB6_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183946.1"
                     /translation="MISRVFKRTVFAAVVAAGILAGTAAHATTLDDVYRAVAQGTRVE
                     AADAKAAVDAMRASAAAAGAARDEAARTKKSLEAEVAALEEAVSAAQERVRERRAKLE
                     KERASVEAVLASIERHEGLLTESVMKTQIGRSKAFMDDAPVSKTDGVARLR"
     gene            complement(459..1223)
                     /locus_tag="ACZPB6_05420"
     CDS             complement(459..1223)
                     /locus_tag="ACZPB6_05420"
                     /inference="COORDINATES: protein motif:HMM:NF023358.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 family protein"
                     /protein_id="HIR1183947.1"
                     /translation="MTNFRKTALALASAFMMAGAAGAAGLPDILERDAAGTSAAQKAG
                     AELARLVDGKDDAEAARRMEEREARLFAHYRDKLAASLSRLEASEAAIEAERESVRRM
                     RMETGPLMDEMAASLAEVVAADRKFLSGERRARLENLRAVLADPTLTDGQKMDVLLDA
                     WQVEASYGLTVGADRGVNDSGALAVFLRIGRLLHAEVSEDGRRARIETADGWRELSEK
                     DAAMLAEAVKTASGAAAPDLMLVAESLLGEAVKEAK"
     gene            complement(1223..4117)
                     /locus_tag="ACZPB6_05425"
     CDS             complement(1223..4117)
                     /locus_tag="ACZPB6_05425"
                     /inference="COORDINATES: protein motif:HMM:NF017044.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR1183948.1"
                     /translation="MNKIRTGLRAALLAAAVCEALFIMTGCTAADRTGGAEAARSEAT
                     VSEGVLPTASAMKRRVLPNGMTVFLYPRRDATGSLEARLVVRAGSLQETEEERGLAHY
                     VEHMAFNGTRDFPDQSAFKALEADGIMLGADVNAVTSLGGTTYRLSVPQATRTGLDTA
                     LHIMREWAFNITFRPEAFEREREIIVEEWRLREGVGARINGPLQTLRYEGSASRDRDP
                     IGLIDVIRTAPVERAKAFYERWYSPQNMTLLLVGAFDEATADELIERYFASEPARGLE
                     TPADWGRFEPVANEDRLTTLVLDPEVSDRFVQIQLQRTLESTSDTVNEAWRETIERLT
                     LDILGRRFALMKENGGQGSLQAPESSWILSPSETQVLLLARPGTDEPLEAALERASGA
                     LKTLAAFGPSKEELDAAVRARRDAVRSRRLAAVRISNASVADDFADAVVYRLPMLDET
                     QQDEMVSAFLAEVTADHVRASASALLASRVKLGAVAGASEKTSKASLRAAWTKGVASP
                     AKPWTEVRRTVSLDVTPPAGPVTAETTVLPSPTGSGRARRFEFANGLKLVIIEDPSLT
                     GRTTLNLRLAGGTSAVDHGLLAVPAALTLPMRSGIGSLTVADIRRVARDAKVSVMSYA
                     EQLHHGIRAEADPEGVPAMMAILHARLSAPRFDEKALADMRRDRIRDLEHAPAERRFM
                     DAIARDAFTAGGTMVAGRDDVDALASTEKLSALEAKLLGDPSRMTVTIVTRESVEKTY
                     EEAAPWLASLAKRGEGFEFWRDQGVRPASGTGVKVWPWATADKAMVQMHYRSAMPWSE
                     ADADRLSLIGYAANRALRESLRTKASGVYVVNLNPLLVKEPAPYFIGRLNFTSAPARA
                     EDLARRADEVLKTLARTGIDAAAFEELKAEWRVRHERDVRDAYYWCESAAMTDGSRTA
                     FEDLAGRTARFEAIGLEDTNRLLATLFGDEPRTYMMTPADRRSAATAGDK"
     gene            complement(4114..5781)
                     /locus_tag="ACZPB6_05430"
     CDS             complement(4114..5781)
                     /locus_tag="ACZPB6_05430"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017775.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HIR1183949.1"
                     /translation="MHQLKTFWRLVRPFWMQSTGRTGARLLLLAVIGLSLSTVWFSVR
                     LNTWNGAFFNAIQAMDGGTIYPLLLEFIAIVAAFVFVLVYADWLRKKLVIEWRRWMTE
                     DLLGRWLSADGRHYRLQLAGLEPDNPDQRIAEDVRLLIESSLTLVISFLRSLLTIVSF
                     VTILWTLSGTLEWTMFGTEWALPGYMVWTCIGYTLVATGLTHVIGRPLMKLNFEQQRR
                     EADFRTSLFDIRRHGESIAGLGAEEAEQARLRASFERIVGNWRSLMNRERDLAFFTVG
                     FGQVTQLVPIFFALPKFLSGAIQLGGLMQIRIAFQQVASAIGWFIYAYRDIAEWSATV
                     DRLAGFEAALSAPLPGSCVRQGERVAACGLVRSKTGEPLLEDLRLGAEPGRLTVVRGA
                     SGIGKTTLLRALAGFWPWVEGHLENRPGAVWLTQRTWLAPMPLRELLAYPHGADAVSR
                     SGCEAALRDAGLERLVPLLDEDEPRSWMNTLSGGEQQRVLFARLLLARPAAVLMDETT
                     SGLDDALAVRLLTLLRERLPDTTFLLVSHQPAVHRIADRLYDLGERA"
     gene            complement(5893..>7629)
                     /locus_tag="ACZPB6_05435"
     CDS             complement(5893..>7629)
                     /locus_tag="ACZPB6_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183950.1"
                     /translation="TPVAGGTQTQRRTADNFFAKATLYATPWTEASLALTYSGYESKG
                     FLTTVADSGYEDHHDGLNLTASVKHRMKAGVLDSSLAYTRMTDKRTSDVKNWTQLDRY
                     TIGMDDTGSTSTTSMTSAASGGLGDLESTQSVINAKTRMDCEPVMVGSVSHQFSAGAD
                     LSSTHAEYRRGSNYYRWGVTQSVMQSDYGEFSQTDFYTTRWKAGTYEADYNQAALFGE
                     HRMGVGDFVIRTGLRAEYDDFTKDVNIAPRFTTSWDLFGNGGSVITVGANRYYGRNIL
                     TYALYKAQNGGMENIYDYASDDSPAADGWFAANDFDGLERLKTPFSDEFSIGLTQRLG
                     DWSASAAYVHRNGHDEVRSRSRTEGSGYDTRFVREFYNGGESEHDSVIFSINNTAPLK
                     FAKANHWVRFSAAWQETKSNAAIDQGYSELESGKTVNMDKVWNDGSVIDAEDLDSTDF
                     NIPVKFDLEATSEWAQWGLTWYNFLHLSLDRSQAVRDDGEYYAWTHEGEYGPMTEQIR
                     KYSRVDFASAWSWDTKVLYRPDWARGVGVSLEVYNVTNNRNASDVFVYKGTQYKSYDP
                     GRQFWVQLSYDY"
BASE COUNT         1184 a         2482 c         2669 g         1294 t
ORIGIN      
        1 gcgcaggcgt gcgacgccgt ccgtcttcga gacgggcgca tcgtccatga aggccttgct
       61 cctgccgatc tgggtcttca tcaccgattc ggtgaggagt ccttcgtgcc tttcgatcga
      121 ggcgagcacg gcctcgaccg aggccctctc cttctcgagc ttcgcgcgcc tctcgcgcac
      181 gcgctcctga gcggccgaaa cggcttcctc aagcgccgca acctcggcct cgagcgactt
      241 cttcgttcgg gcggcctcgt cacgggccgc gcctgcagcg gcggcggacg ccctcatggc
      301 atcgacggcc gccttcgcat cagccgcctc gaccctcgtc ccctgagcga cagctcggta
      361 tacgtcgtca agcgtggtgg cgtgcgcagc cgttccggca agaatgccgg cagcaacgac
      421 tgcggcaaaa acggtcctct tgaaaacacg ggagatcatc acttcgcctc cttcacggcc
      481 tcgccgagga gcgactcggc gacgagcatc agatcgggcg cagcggcgcc cgaagcagtc
      541 ttcacggctt cggcgagcat ggcggcatcc ttttcggaga gctcgcgcca gccgtccgcg
      601 gtctcgatgc gagctcggcg cccgtcctcg gagacctctg cgtgaagaag cctgccgatg
      661 cgcaggaaga cggcgagcgc gcccgagtcg ttgacaccgc ggtcggcgcc gaccgtgagg
      721 ccgtagctcg cctccacctg ccaggcatca aggagtacgt ccatcttctg gccgtcggtg
      781 agcgtcggat cggcaagcac ggcgcgcagg ttctcgagcc tcgcccggcg ttcgcccgaa
      841 agaaacttcc tgtccgccgc cacgacctcg gcgagcgagg ccgccatctc gtccatgagg
      901 ggcccagtct ccatgcgcat tcggcgcacg gactcacgct ccgcctcgat ggcggcctcg
      961 gaggcctcga gacgcgagag ggatgcggcg agcttatcgc ggtagtgcgc aaaaagcctt
     1021 gcctcgcgct cctccatccg ccttgcggcc tcggcgtcgt cctttccgtc gacgaggcgg
     1081 gcgagctcgg cgcccgcctt ctgcgcggca gacgtgcccg cggcgtcacg ctcgagaatg
     1141 tcgggcaggc cggccgcgcc tgcggcaccc gccatcatga aggccgacgc aagcgcgagg
     1201 gccgttttcc tgaagttcgt catcacttgt ctccggccgt cgcggcgctc cttcggtccg
     1261 cgggcgtcat catgtaggtg cggggctcgt ccccgaaaag cgttgcgaga agtctgttcg
     1321 tgtcctcgag gccgatcgcc tcgaaccgtg cggtcctccc cgcgagatcc tcgaaggccg
     1381 tcctcgagcc gtccgtcatg gcggcggact cgcaccagta gtaggcgtcg cgcacgtcgc
     1441 gctcatggcg cacgcgccac tcggccttga gctcctcgaa ggccgccgcg tcgatgcccg
     1501 tgcgggcgag tgtcttcaga acctcgtcgg cgcgccttgc cagatcctcg gccctcgcgg
     1561 gcgcggacgt gaagttgagg cggccgatga agtacggggc gggctccttc acgaggagcg
     1621 gattgaggtt cacaacgtag acgcccgagg ccttcgtcct gagcgactcg cgcagcgcgc
     1681 ggttcgccgc atagccgatg aggctcagcc tgtcggcgtc ggcctcgctc cagggcatgg
     1741 cggaccgata gtgcatctgc accatggcct tgtcggccgt ggcccagggc cagaccttca
     1801 cgcccgtgcc ggacgcgggc ctcacgccct gatcgcgcca gaactcgaaa ccctcgccgc
     1861 gcttggcgag cgaggcgagc cagggtgccg cctcttcata ggtcttctcg acggactccc
     1921 tcgtaacgat cgtcaccgtc atgcgcgacg ggtcgccgag gagcttcgcc tcaagggcgg
     1981 aaagcttctc ggtcgaggcg agcgcatcca cgtcgtccct gcctgcgacc atcgtgccgc
     2041 ccgccgtgaa cgcatcgcgg gcgattgcgt ccatgaagcg gcgctcggcg ggtgcatgct
     2101 cgaggtccct gatgcggtcg cgccgcatgt cggcgagcgc cttttcgtcg aacctcggag
     2161 cggagagcct ggcgtgcagg atcgccatca tggcgggcac gccctcaggg tcggcctcgg
     2221 ccctgatgcc gtggtggagc tgttcggcgt agctcatcac cgagaccttt gcgtcccttg
     2281 caacacggcg aatgtcggcg accgtaagag agccgatccc ggacctcatc ggcagcgtga
     2341 gtgcggcggg cacggcgaga aggccgtgat cgaccgcgga cgtgccgccc gcaagcctca
     2401 ggttgagggt cgtcctgccc gtgagcgacg gatcctcgat gatcacgagc ttcaggccgt
     2461 tggcgaattc gaatcgacgg gcgcggcccg agcccgtggg cgacggcaga accgtcgttt
     2521 cggccgtgac gggaccggcg ggcggcgtga catcgagcga cacggtcctc ctcacctcgg
     2581 tccagggctt tgcgggcgag gcgacgccct tcgtccaagc ggcgcgaagc gaggccttcg
     2641 acgtcttctc ggaggcgccc gcaacggcgc cgagcttgac gcgggaggca aggagcgctg
     2701 aggcggaggc cctgacgtgg tccgccgtca cctcggcaag gaaggccgag accatttcgt
     2761 cctgctgcgt ctcgtcgagc atcggcagcc ggtagacgac ggcgtcggcg aagtcgtcgg
     2821 cgacgctcgc gttcgaaatg cgcacggcgg cgagcctgcg gcttcggacc gcgtcgcgtc
     2881 ttgccctcac ggcggcgtcg agctcctcct tcgagggtcc gaaggcggcg agcgtcttca
     2941 gggcacccga ggcgcgctca agtgcggcct caagcggctc gtccgtgccg gggcgcgcaa
     3001 ggagcagaac ctgcgtctcg gagggcgaca ggatccagct cgactcgggc gcctgaagcg
     3061 agccctgtcc gccgttttcc ttcatgagcg cgaagcggcg gccgaggatg tcgagggtga
     3121 ggcgctcgat cgtctcgcgc cacgcctcgt tcaccgtgtc ggaggtcgac tcgagcgtgc
     3181 gctggagttg gatctgcacg aagcggtccg agacctcggg gtcgagcacg agcgtcgtca
     3241 ggcgatcctc gtttgcaacg ggttcgaagc ggccccagtc ggcgggcgtc tcgagcccgc
     3301 gggcaggttc ggaggcgaaa tagcgctcga tgagctcgtc cgccgttgcc tcgtcgaagg
     3361 cgcccacgag aaggagcgtc atgttctgcg gcgagtacca gcgctcgtag aaggccttcg
     3421 cgcgctcgac gggcgccgtg cggatcacgt cgatgagacc gatcggatcg cggtcgcgcg
     3481 aagcgcttcc ttcgtagcga agggtctgga gcggcccgtt gatgcgtgcg cccacgcctt
     3541 ccctcaggcg ccactcctcg acgatgatct cgcgttcacg ttcgaaggcc tcgggcctga
     3601 acgtgatgtt gaaggcccat tcgcgcatga tgtgcagagc cgtgtcgagc cccgtgcggg
     3661 tggcctgggg gacggagagc ctgtaggtgg tgccgccgag agacgtgacg gcgttgacgt
     3721 ccgcgccgag catgatgccg tcggcctcaa gcgccttgaa ggcgctctgg tcggggaagt
     3781 cacgcgtgcc gttgaaggcc atgtgctcga cgtagtgtgc aagcccgcgc tcctcctcgg
     3841 tctcctgaag gctgcccgcg cgcacgacga ggcgcgcctc gaggctgccc gtcgcatccc
     3901 tgcgcggata aaggaacacc gtcatgccgt tgggcagaac acggcgcttc atggcggatg
     3961 ccgtcggaag cacgccttcg ctcacggtcg cctcgctgcg ggcggcttcg gcgccgcccg
     4021 tgcggtccgc ggcggtgcag cccgtcatga tgaaaagcgc ctcgcacacg gccgcggcca
     4081 gcagtgcggc gcgcaggcct gtcctgattt tgttcatgct cgttctccga gatcgtagag
     4141 gcggtccgca atgcgatgga ccgcgggctg gtgcgagacc agaaggaagg tggtgtcggg
     4201 gagccgctcc ctgaggagcg tcagaagcct gacggcgagc gcgtcgtcga gccccgaggt
     4261 cgtctcgtcc atgaggacgg cggcgggtct tgcgagcagc aggcgcgcga aaagcacgcg
     4321 ctgctgctcg ccgcccgaga gcgtgttcat ccagctgcgg ggttcatctt cgtcaagaag
     4381 cgggacgagc cgctctagcc ctgcgtcgcg aagcgcggcc tcgcagccgc tcctcgagac
     4441 ggcatcagca ccgtgcggat aggcaagcag ctctctgagc ggcatcggcg cgagccacgt
     4501 gcgctgcgtg agccagacgg cgcccggcct gttttcaaga tgtccctcga cccaaggcca
     4561 gaagcccgcg agggcgcgaa ggagcgtggt cttgccgatg ccggaagcac ccctcacgac
     4621 ggtgaggcgg ccgggctcgg ccccgaggcg gaggtcttca agaagcggct cgcccgtctt
     4681 cgagcgcacg aggccgcagg cggcgacgcg ctcgccctgc ctgacgcagc ttcccggaag
     4741 gggcgcactc agggcggcct cgaagcccgc gagtcggtcg accgtggccg accactcggc
     4801 gatgtcgcgg taagcgtaga tgaaccagcc gatagcggag gcgacctgct ggaaggcgat
     4861 gcggatctgc atgaggccgc cgagctggat ggcgcccgag aggaacttcg ggagcgcgaa
     4921 gaagatgggc acgagctgcg tcacctggcc gaagcccacg gtgaagaatg cgaggtctcg
     4981 ctccctgttc atgagcgagc gccagttgcc gacaatgcgc tcgaacgaag ccctgagcct
     5041 cgcctgctcg gcctcctcgg cgcccaggcc cgcaatcgac tcgccgtggc ggcggatgtc
     5101 gaagagcgag gtgcggaagt cggcctcccg cctctgctgc tcgaagttga gcttcatcag
     5161 gggacggccg atcacgtgcg tgagccccgt cgcgacgagc gtgtagccga tgcaggtcca
     5221 gaccatgtag ccgggaagcg cccactcggt gccgaacatc gtccactcga gcgtgcccga
     5281 cagcgtccag aggatggtga cgaagctcac gatggtgagg agcgagcgca ggaaggagat
     5341 gacgagcgtg agcgacgatt cgatcaggag ccgcacgtcc tcggcaattc gctggtcggg
     5401 gttgtcgggc tcaaggcccg cgagctgcag gcggtagtgg cggccgtcgg cggagagcca
     5461 acggccgagc aggtcctcgg tcatccagcg gcgccactcg atcacgagct tcttgcgcag
     5521 ccagtccgca tagacgagga cgaacacgaa ggccgcgacg atcgcgatga attccagaag
     5581 aagcgggtag atcgtcccgc cgtccatggc ctgaatggca ttgaagaagg cgccgttcca
     5641 ggtgttgagg cgcacggaga accagacggt cgagagcgac aggccgatga cggccagaag
     5701 cagcaggcga gcgcccgtgc gccctgtgct ctgcatccag aagggacgca cgagccgcca
     5761 gaaggtcttg agctgatgca tgggacttgt attgaagtga ggagcgggcc gggcggcgga
     5821 gtcgtccgcc gtccggccgg gtcaggattt gaatgagccc cggctgagcc tcggctgaag
     5881 ccggagcgcg gatcagtagt cgtaggagag ctggacccag aactggcggc ccggatcgta
     5941 gctcttgtac tgggtgccct tgtagacgaa gacgtcagag gcgttgcggt tgttggtcac
     6001 gttgtagacc tcgagcgaga cgcccacgcc gcgggcccag tcgggcctgt agaggacctt
     6061 cgtgtcccag gaccaggcgg aggcgaagtc gacgcggctg tacttgcgga tctgctcggt
     6121 catcggaccg tactcgccct cgtgcgtcca ggcgtagtac tcgccgtcgt cgcgcacggc
     6181 ctggctacgg tcgagcgaga ggtgcaggaa gttgtaccag gtgagtcccc actgagccca
     6241 ttcggacgtt gcctcgaggt cgaacttcac aggaatgttg aagtcggtcg agtctaggtc
     6301 ctcggcgtcg atcacgctgc cgtcgttcca gaccttgtcc atgttgacgg tcttgccgga
     6361 ctcgagctcg ctgtagccct ggtcgattgc tgcgttgctc ttcgtctcct gccatgcggc
     6421 ggagaagcgc acccagtggt tcgccttcgc gaacttgagg ggcgcggtgt tgttgatgct
     6481 gaagatcacg gaatcgtgct cggactcgcc gccgttgtag aactcgcgca caaagcgcgt
     6541 gtcgtagccg ctgccctcgg tcctcgagcg cgagcgcacc tcgtcgtggc cgttgcggtg
     6601 cacgtaggcg gcggatgcgg accagtcgcc gaggcgctgc gtgaggccga tgctgaactc
     6661 gtcgctgaag ggggtcttca ggcgctcgag gccgtcgaag tcgttggcgg cgaaccagcc
     6721 gtcggcggcg ggactgtcgt cggaggcgta gtcgtagatg ttctccatcc cgccgttctg
     6781 cgctttgtag agcgcgtagg tgaggatgtt gcggccgtag tagcggttgg cgcccacggt
     6841 gatgacggag ccgccgttgc cgaagaggtc ccacgaggtc gtgaagcgcg gcgcgatgtt
     6901 gacgtccttc gtgaagtcgt cgtactcggc gcgcaggccc gtgcggatca cgaagtcgcc
     6961 cacgcccatc ctgtgctcgc cgaagagcgc cgcctggttg tagtcggcct cgtacgtgcc
     7021 cgccttccag cgcgtcgtgt agaagtcggt ctgcgagaat tcgccgtagt ccgactgcat
     7081 cacggactgc gtgacgcccc agcggtagta gttgctgccg cgcctgtact cggcgtgcgt
     7141 cgaggagagg tccgcgcccg cgctgaactg atggctgacg ctgccgacca tgacgggctc
     7201 gcagtccatc ctcgtcttcg cgttgatgac ggactgcgtg ctctcgaggt ccccgagacc
     7261 gcccgaggcg gccgaggtca tgctcgtcgt cgaggtgctc cccgtgtcgt ccatgccgat
     7321 cgtatagcgg tcgagctgcg tccagttctt gacgtcgctc gtgcgcttgt cggtcatgcg
     7381 cgtgtacgcg agggacgagt cgagcacgcc cgccttcatg cggtgcttga cgctggccgt
     7441 caggttgagg ccgtcgtggt gatcctcgta gccggagtcg gcgactgtcg tgaggaagcc
     7501 cttcgactcg tagcccgaat aggtgagcgc gagactcgcc tccgtccagg gcgtcgcgta
     7561 gagcgtggcc ttcgcgaaga agttgtcggc cgtgcggcgc tgcgtctgcg tgccgcccgc
     7621 gaccggcgt
//
LOCUS       DBMDTI010000086         7536 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2612_length_7536_cov_3.475588, whole genome shotgun sequence.
ACCESSION   DBMDTI010000086 DBMDTI010000000
VERSION     DBMDTI010000086.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7536)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2612_length_7536_cov_3.475588"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="ACZPB6_05440"
     CDS             complement(<1..437)
                     /locus_tag="ACZPB6_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1183951.1"
                     /translation="MKKDLTQEEIPVWVVDDEVRYSIRFALATHGIDARTYASGEDFF
                     NLVETDQPGCLVVDLTMPGMSGIEVLKRLRDLDSPIKVIVLSGHGTIRTAVEAMENGA
                     ISFMEKPVDPDDLLVKINAARDASLLEVCKTRVRHMLRQFSKR"
     gene            642..1709
                     /locus_tag="ACZPB6_05445"
     CDS             642..1709
                     /locus_tag="ACZPB6_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014017.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR1183952.1"
                     /translation="MMKGKPMIERSREPLFDDAPRPDAPVSGEPPRRSVLEASARLSR
                     LVRLGTLGFGRPYWRGTIYSASSGALRMKGLDGLSVLARTPWLGCVCLERGYLHPHSQ
                     AELAVLASAVPADFRFIVRAPALVTSVFVHDRRGRAGGLNREFLNVAAAAAFVRSCTD
                     GLGEKLGGVLFDFGPYPSSQMKMLQGRQKAVEELGAFAEGLVRELGSADAAPVLAFEV
                     RNPTLLTPRLMALLRNFGIRPVMGLNEGMPGLQRQMRALAACDAQDPSDPDWRLSGPL
                     FVRWHRSGPLSPVFVRDPESKSAGDPVTRTLIASLVMRAVRSGVPAYVLAGDDAEGDA
                     PRTLLDILASLDGMRAAGLRR"
     gene            complement(1714..2271)
                     /locus_tag="ACZPB6_05450"
     CDS             complement(1714..2271)
                     /locus_tag="ACZPB6_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1183953.1"
                     /translation="MNESFFKMILSRHSLGAKHLVIPAPDVGALRLAVTAACRVPCHQ
                     ETLPFRWVEISSRDRLADLFESVLPADADEEMRAKARGKALKAPMCMALVGTGLSPDS
                     QDRDADERLMTAGASLMNFLAGLHAQGFAAKAVSAKDFPAPDGLYDPTCERLLCFVLV
                     GTPDTPVNYQDIGMGNDERQPLSRW"
     gene            2365..2997
                     /locus_tag="ACZPB6_05455"
     CDS             2365..2997
                     /locus_tag="ACZPB6_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - L-tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR1183954.1"
                     /translation="MRSLWDISPSVSTRAPVFPGDTPFQVLWNERINDGGVANISTIH
                     MSPHVGAHVDAPMHLDSGAVDVATLRLERFIGRCRVIDLSGADTTEPVSREELSSACF
                     HERVLIKTRRTAPERWTEDFRSVSPGAVRMLLESGVMLIGLDTPSIDPADSTLLLSHR
                     VALQGGISIIENLDLSQVEAGDYELIALPLKLEGVEASPVRAVLRSVAHR"
     gene            3007..3459
                     /locus_tag="ACZPB6_05460"
     CDS             3007..3459
                     /locus_tag="ACZPB6_05460"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR1183955.1"
                     /translation="MSGKVFEQPLRIRWNHCDPAGIVYHPQYFVMLNNAMEDFFRECA
                     GLSYEESLVEGYGFPIAGIRCDFCAPSRCGDDCVLRMWIESIGTTSVRFAMTIHAGDE
                     CRLACIETAVCAAREGDAVVKRPIPAEMRGRLEEYLKAPEVPELALRA"
     gene            complement(3449..4255)
                     /gene="murI"
                     /locus_tag="ACZPB6_05465"
     CDS             complement(3449..4255)
                     /gene="murI"
                     /locus_tag="ACZPB6_05465"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR1183956.1"
                     /translation="MTDTRPVGILDSGFGGLSVARAVAELLPQENLVYAADCGFAPWG
                     DRTDDFILERTHALVSHLLRKDVKAIVIACNTATAVCAESLRAELSMPVVGIEPAVLP
                     GARTTRTGIIGVMATTKTLASAKYRHLKSIAPKEVRIIDCPCPGLMDCVEAGEFRTPH
                     TMALLREYVEPLVEAGADTLVLGCTHYPFLSDAIRLTAGPGIRLIDPAPAVARQLKRR
                     LDEPGLLNDTKKAPARIFCTTDANEARERILRLLWTDNAVLQKLDGSVRP"
     gene            complement(4252..5175)
                     /locus_tag="ACZPB6_05470"
     CDS             complement(4252..5175)
                     /locus_tag="ACZPB6_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1183957.1"
                     /translation="MHKSTLETHHQPIMPVLALVLATFFWGSSFITVGSALEYTNPLT
                     LVMLRFGVAAFFVLLMLKGRIADIPKRTWKAGAACAFLIYLGYTTNAAGLMTLQSSES
                     GFLTALYVPITPLLVWVVFGKAPTKGAVFGVMMAFAGLVLLANPFTLSFSNNWGEWVT
                     ILSALISAVEIMVVGRVAPGTQARQLAFTQLFFTAVFAAVGLAVATAAGVPLKETTFN
                     TTVVGGILWLAVIVAFVQVLLSWAQRYVPAGRAAVIFAMESVFAAIIGWFAGESLGTA
                     GIIGGVLIVGGILCGEIRLPRRKAAPKELSS"
     gene            complement(5235..5588)
                     /locus_tag="ACZPB6_05475"
     CDS             complement(5235..5588)
                     /locus_tag="ACZPB6_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HIR1183958.1"
                     /translation="MNKIYGIKNCGSVKKALEWFVARDLPFEFHDFRKEGIDPVLLDE
                     WIAKTSCDVLLNRKGLLWRKLDKARREAVCDNPEGMRALMLENPVIIKRPVIVHPDGS
                     VTVGVDEEAWERLAG"
     gene            complement(5601..6596)
                     /locus_tag="ACZPB6_05480"
     CDS             complement(5601..6596)
                     /locus_tag="ACZPB6_05480"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR1183959.1"
                     /translation="MTQTPPVDEVRIAEAVRVLEAGGLVAMPTETVYGLAADADNESA
                     VLSTYAAKGRPADHPLIVHVAGIDAISHWAEDVPPEAWLLAKKYWPGPLTLVLKKKAR
                     CGLWVTGGQDTVALRCPSHPWAHALISAFSGPTWRGLTAPSANTFGRISPSCAAHVAE
                     DLGVKPEGKLDMILDGGSCEFGIESTMINLSQGRPTLLRHGVVTREMVEETLGFAVPD
                     AGKDAPRASGRLKSHYAPRTKLELVSAEALPARFAEMGAAPVAVMASGEVLDHLEGTP
                     VLAIKAPADLHGYGRDLYENLHRLDRAGAERILVELPPQSPDWAAVNDRLGRAAA"
     gene            complement(6598..>7536)
                     /locus_tag="ACZPB6_05485"
     CDS             complement(6598..>7536)
                     /locus_tag="ACZPB6_05485"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIR1183960.1"
                     /translation="FENVPAPSLEYLEKAGLRVCPPSAAVAVTQDRNVEKHFVSEVAG
                     VPVAPHAAVRSEADVDALDASLLPGILKTARLGYDGKGQARVATIEDVRKAFRDFGGV
                     DCVLEKRLSLKLEVSVIVARNSHGETATFPVSENFHRNGILAVSVLPARIDEDTASRA
                     RGYAAKIAQALNYVGVLCVELFILEDGTILVNELAPRPHNSGHATIDACVTSQYEQQV
                     RTMAGLPLGSTEQHSHAVMLNILGDEWFDEEGVKREPDWAGVTAIPGAKLHIYGKADA
                     RRARKMGHVTCIGRTAEEAMERAREAARILHLPEPN"
BASE COUNT         1247 a         2304 c         2694 g         1291 t
ORIGIN      
        1 tcgcgcttgg agaactggcg gagcatgtgg cgcacgcgcg tcttgcagac ctcgagaagg
       61 gaggcgtctc gtgcggcgtt gatcttcacg agaaggtcgt cgggatcgac gggcttctcc
      121 atgaaggaga tcgcgccgtt ctccatggct tcgacggccg tgcggatagt gccgtggccg
      181 gaaagcacga tgaccttgat ggggctgtcg agatcgcgaa ggcgcttcag aacctcgatg
      241 ccgctcatgc cgggcatggt caggtcgacg acgaggcagc cgggctggtc cgtttcgacg
      301 aggttgaaga agtcttcgcc gctggcgtag gtgcgtgcat cgatgccgtg cgtcgcaaga
      361 gcaaagcgga tcgaatagcg gacctcgtcg tcgacgaccc agacggggat ttcctcctgc
      421 gtcaggtcct tcttcatggc cttctcttcg gaactatcag tgatgggcat atttcaatta
      481 atatcgcttg agcaggcctg cggcgtacgg atgtacggga gacgaacgcg gccgcgaata
      541 tcattcaatg ctaccaaaaa agaggtcgtc tgagtgccct cgctgggatg cctgcgatgt
      601 caggacgcac gtacgcgaga gcatgtcagg acgaaaacgc catgatgaag ggcaagccga
      661 tgattgagag aagcagggag ccgctctttg acgatgcgcc gcgtccggac gcgcccgtct
      721 cgggcgagcc gccccgcagg agcgtgctcg aggcttcggc acgtctttcg aggctcgtgc
      781 gtctgggcac gctcggcttc gggcggccgt actggcgcgg caccatatac tcggcctcga
      841 gcggcgcgct ccgcatgaag ggactagacg gcctgtccgt gctcgcgcga acgccctggc
      901 tgggctgcgt ctgcctcgag cgcggatacc tccatcccca ttcacaggcg gagcttgccg
      961 ttctggcgtc cgccgttccc gccgacttcc gcttcatcgt gcgcgcgccc gcgctcgtga
     1021 cgagcgtctt cgtgcacgac agacgcggcc gtgcaggcgg cctcaacagg gagttcctca
     1081 acgtggcggc cgccgcagcc ttcgtgagaa gctgcacgga cggactcggc gagaagctcg
     1141 gcggcgtgct cttcgacttc gggccctatc cgtcctcgca gatgaagatg cttcagggca
     1201 ggcagaaggc cgtggaggag ctgggtgcct tcgcagaggg gctggtgcgg gagctgggct
     1261 ctgcggacgc cgcccccgtg ctcgccttcg aggtgagaaa tccgacgctg ctcacgccgc
     1321 gcctcatggc gctcctgaga aacttcggca tcaggcccgt gatgggactc aatgagggca
     1381 tgcccggact gcagcgccag atgcgggcgc ttgccgcctg cgacgcgcag gatccctcgg
     1441 atcccgactg gaggctttcg gggccgctct tcgtgcgttg gcatcgttcg ggcccgcttt
     1501 cgcctgtctt cgtgagggac ccggaaagca aaagtgcggg cgaccccgtc acccgcactc
     1561 tgattgcatc cctcgtcatg cgcgccgttc gcagcggcgt gcccgcctac gtgctggcgg
     1621 gcgacgatgc cgaaggcgat gcgccgcgca cgcttctgga catcctggcc tcgctcgacg
     1681 gcatgcgcgc cgccgggctc aggcgctgac gtcctaccag cgggagagcg gctggcgttc
     1741 gtcgttgccc atgccgatgt cctgatagtt gacgggcgtg tcgggcgttc ccacgagcac
     1801 gaagcagagc aggcgctcgc aggtcggatc gtaaaggccg tcgggggcgg ggaagtcctt
     1861 ggcggagact gccttcgcgg caaagccctg ggcatgcagg cctgcaagga agttcatgag
     1921 cgaggcgcct gccgtcatca ggcgttcgtc ggcgtctcgg tcctgggagt cgggggagag
     1981 gcccgtgccc acgagcgcca tgcacatcgg cgccttcagg gccttgccgc gggccttggc
     2041 gcgcatctcc tcgtcggcgt ccgcaggcag aaccgattcg aagaggtcgg cgagacggtc
     2101 gcgggaggag atctcgaccc agcggaaggg aagcgtctcc tgatggcagg gcacgcggca
     2161 ggcggccgtc acggccagac gcagggcgcc cacgtcaggg gcgggaatga cgagatgctt
     2221 ggcgccaagg gagtggcgcg acaggatcat tttgaagaaa ctttcattca cggcgtaaat
     2281 ctccgacatt gccggctgga aaacgtgcgg ccggacacgc agaatattat gctacgaaga
     2341 caagaacatt caggaggcca ctgcatgcgc agcctttggg acatttcccc ttccgtaagc
     2401 acccgagcgc ccgtattccc gggcgacacg ccgttccagg tcctctggaa cgagcgcatc
     2461 aacgacggcg gagttgccaa catctcgacg atccacatgt cgccgcacgt gggcgcgcat
     2521 gtcgacgccc cgatgcatct cgacagcggc gccgtggacg tcgccacgct caggctcgag
     2581 cgcttcatcg gccgctgccg cgtcatcgat ctgagcggtg cggacacgac agagcccgtc
     2641 agccgcgagg aactctcgag cgcctgcttc cacgagcgcg tcctcatcaa gacccgcagg
     2701 accgcgcccg agcgttggac ggaggacttc aggtccgtct ctcccggcgc cgtgcgcatg
     2761 ctcctcgagt cgggcgtgat gctcataggg ctcgacaccc cgagcatcga ccccgccgac
     2821 agcacgctcc tgctttcgca ccgcgtggcc cttcagggcg gcatctccat catcgagaac
     2881 ctcgacctga gccaggtcga ggcgggcgat tacgagctca ttgcgctgcc cctcaagctc
     2941 gagggcgtcg aggccagtcc cgtgcgcgcc gtcctgcgct ccgttgcaca tcgatgaggt
     3001 ggcgtcatga gcggaaaggt ttttgagcag ccccttcgca ttcgctggaa ccactgtgat
     3061 ccggcgggca tcgtctacca tccccagtac ttcgtgatgc tcaacaacgc catggaggac
     3121 ttcttccgcg agtgtgcggg cctgagctac gaggagagcc tcgtcgaggg ctacgggttc
     3181 ccgatcgcgg ggatccggtg cgacttctgc gctccgagcc gatgcgggga tgactgcgtg
     3241 ctgcgcatgt ggatcgagag catcggcacg acgtccgtgc gcttcgccat gacgatccac
     3301 gcgggcgacg aatgcaggct cgcctgcatc gagacggcgg tctgcgccgc acgcgagggc
     3361 gacgccgtcg tcaagcgtcc gattcccgcc gaaatgagag ggcgcctcga ggagtatctc
     3421 aaggcgcctg aagttccgga gctggccctc agggcctgac gctcccgtcg agcttctgca
     3481 ggacggcgtt gtccgtccag aggagcctga gaatgcgctc gcgcgcctca ttggcgtccg
     3541 tcgtgcagaa gatgcgcgcg ggcgcctttt tcgtgtcgtt gaggaggccc ggctcgtcaa
     3601 ggcggcgctt gagctgacgc gcgacggcgg gggcgggatc gatgagcctg atgcccggtc
     3661 ccgcagtgag gcggatggca tcggagagga agggatagtg cgtgcagccg agaacgagcg
     3721 tgtcggcgcc cgcctcgacg agcggctcca cgtactccct gagaagggcc atcgtgtgcg
     3781 gcgtgcggaa ttcgcccgct tccacgcagt ccatgaggcc ggggcacgga cagtcaatga
     3841 tcctgacctc cttgggggcg atggacttga ggtggcggta tttggcgctt gcgagcgtct
     3901 tcgtcgtcgc catcacgccg atgatgcccg tcctggtcgt gcgggcgccg ggaagcacgg
     3961 cgggctcgat gcccacgacg ggcatggaga gctcggcgcg caggctttcc gcacagacgg
     4021 ccgtggccgt gttgcaggcg atgacgatgg ccttcacgtc cttcctgaga aggtgtgaga
     4081 cgagcgcatg cgtgcgctcg agaatgaagt cgtccgttct gtcgccccag ggggcgaagc
     4141 cgcagtcggc ggcgtagacg aggttttcct gcggaagcag ctcggcgacg gcgcgggcga
     4201 cggagaggcc tccgaagccc gagtcgagaa tgccgacggg acgggtgtcc gtcatgacga
     4261 aagctccttg ggtgcggcct tcctgcgcgg aagcctgatt tcgccgcaga gaatgccgcc
     4321 cacgatcagg acgccgccga tgatgcccgc cgttccgagg ctttcgcctg cgaaccaacc
     4381 gatgatggcg gcaaagacgc tctccatggc gaagatgacg gccgcgcggc ccgcaggtac
     4441 gtagcgctgc gcccaggaga gcagcacctg cacgaaggcg acgataacgg caagccagag
     4501 gatgccgccc accaccgtcg tgttgaaggt cgtctccttg agcgggacgc ctgccgccgt
     4561 ggcgacggcc aggccgacgg ctgcaaagac ggcggtgaag aaaagctggg tgaatgcgag
     4621 ctgcctcgcc tgagtgcccg gcgcgacgcg gcccaccacc atgatttcga cagcggagat
     4681 gagggccgag agaatcgtga cccactcgcc ccagttgttc gagaagctga gcgtgaaggg
     4741 gttggcgagc aggacgaggc ccgcaaacgc catcatcacg ccgaagacgg ctcccttagt
     4801 gggcgccttg ccgaagacca cccagacgag aagcggcgtg atcggcacgt agagcgccgt
     4861 gagaaagcca gactcggagc tctgcagggt catgaggccc gcggcgttcg tcgtgtagcc
     4921 cagatagatg aggaaggcgc aggccgcccc agccttccag gtgcgcttgg gaatgtccgc
     4981 aatccgcccc ttgagcatga gcaggacgaa gaaggcggcc acgccaaacc tgagcatcac
     5041 gagcgtgagc gggttcgtgt actcgagcgc ggagccgacc gtgatgaagg agctccccca
     5101 gaagaaggtg gcgaggacga gcgccagcac gggcatgatc ggctggtggt gggtttccaa
     5161 tgtcgactta tgcatctttc agaaaaaagg ccgccgttgc cggcggccgt cgttcggatt
     5221 gtcggtggcc cgcgtcagcc ggcgagcctt tcccaggctt cctcgtcaac gccgacggtc
     5281 acgctgccgt cgggatggac gatcacggga cgcttgatga tgacggggtt ctcgagcatc
     5341 agggcgcgca tgccttcggg attgtcgcac acagcctcgc gtctggcttt gtcaagcttg
     5401 cgccagagca ggcccttgcg gttgaggagc acgtcgcagg aggtcttcgc gatccactcg
     5461 tcaaggagga cgggatcgat cccctccttc ctgaagtcgt ggaactcgaa cgggaggtct
     5521 cgggcgacga accattcgag ggccttcttg acagagccgc agttcttgat gccgtagatc
     5581 ttgttcatgt cgcgttctcc tcaggctgcg gcgcggccga ggcggtcgtt gactgcggcc
     5641 cagtcgggcg actggggcgg cagttcgacg aggatgcgtt cggcgcctgc gcggtcgagc
     5701 ctgtgcaggt tttcgtagag gtcgcgtccg tagccgtgaa ggtcggcggg ggccttgatc
     5761 gcgaggacgg gcgtgccctc gaggtggtca agcacctcgc cggaggccat gacggcgacc
     5821 ggagcggcgc ccatttcggc gaatcgggcg ggcagggctt cggcggacac gagctcgagc
     5881 ttagtgcggg gcgcatagtg gctcttgagg cggccggagg cgcggggcgc atccttgcct
     5941 gcgtcgggca ctgcaaagcc gagcgtctct tcgaccatct cgcgggtgac gacgccgtgg
     6001 cgcaggagcg tggggcgtcc ctgcgagagg ttgatcatcg tggactcgat gccgaactcg
     6061 cagctgccgc cgtcgaggat catgtcgagc ttgccttcgg gcttgacgcc gaggtcttcg
     6121 gcgacgtgtg cggcgcacga cgggctgatg cgcccgaatg tgttggccga aggcgccgtg
     6181 aggccgcgcc aggtcggacc cgagaaggcc gagatgagcg catgagccca gggatgggac
     6241 gggcagcgga gcgccaccgt gtcctgtcct cccgtcaccc agagtccgca gcgcgccttc
     6301 ttcttgagaa cgagcgtgag cgggcccggc cagtacttct tggccagcag ccaggcttcg
     6361 ggcggaacgt cctcggccca gtgggagatg gcgtcgatgc ctgcgacgtg gacgatcagc
     6421 ggatggtccg ccgggcggcc cttggccgcg taggtggaga gcactgcgga ttcgttgtcg
     6481 gcgtcggcgg cgaggccgta gacggtttcg gtcggcatgg cgacgaggcc gcccgcctca
     6541 agaacgcgga cggcttcggc gattctgact tcgtcgacgg gaggagtctg ggtcatctca
     6601 gttcggttcc ggaagatgaa ggatgcgggc ggcttcgcgg gcgcgctcca tggcttcttc
     6661 ggcggtcctg ccgatgcagg tcacgtggcc catcttgcgg gcgcggcggg cgtcggcctt
     6721 gccgtagatg tggagcttgg cgccgggaat ggcggtgacg ccggcccagt cgggctcgcg
     6781 cttgacgcct tcctcgtcga accactcgtc gccgaggatg ttgagcatca cggcgtggga
     6841 gtgttgctcc gtggagccga gcggaaggcc ggccatcgtg cggacctgct gttcgtactg
     6901 gctcgtgacg cacgcgtcga tcgtggcgtg gccggagttg tgcggacggg gagcaagctc
     6961 gttgacgagg atcgtgccgt cttcaaggat gaagagctcg acgcagagga cgccgacgta
     7021 gttgagcgcc tgggcgatct tggcggcata gccgcgggcg cgggaggccg tatcctcgtc
     7081 gatgcgggcg ggaaggacgg agaccgcgag gatgccgttg cggtggaagt tctcggaaac
     7141 ggggaacgtg gccgtttcgc cgtgcgagtt gcgcgcgacg atgacggaga cctcaagctt
     7201 gagggagagg cgcttttcga gcacgcagtc cacgccgccg aagtcgcgga aggccttgcg
     7261 gacgtcctcg atcgtggcga ctcgggcctg gcctttgccg tcatagccga ggcgggcggt
     7321 cttgagaatg ccgggcagga gcgaggcgtc gagggcgtcg acgtcggctt cgctgcgcac
     7381 ggcggcatgc ggggcgacgg gcacgccggc cacttcggag acgaagtgct tctcgacgtt
     7441 gcggtcctgg gtgacggcga cggcggcgga cggcgggcag acgcgcaggc ccgccttctc
     7501 gaggtattcg agcgaggggg cggggacgtt ctcgaa
//
LOCUS       DBMDTI010000087         7534 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2615_length_7534_cov_3.932867, whole genome shotgun sequence.
ACCESSION   DBMDTI010000087 DBMDTI010000000
VERSION     DBMDTI010000087.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2615_length_7534_cov_3.932867"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="ACZPB6_05490"
     CDS             <1..438
                     /locus_tag="ACZPB6_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR1183961.1"
                     /translation="TKEDFARSHPGGALGRRLLIHVQDIMRTGEDIPAVREEVGVLEA
                     VREITKKHIGMTAVVDDQGHVTGIFTEGDLRRLIERLGDIRPCVMKDVMIRNPKTIRA
                     QELAASAVKLLDTFHCNQLLVVDEDNRLIGALHLHDLMGAKVL"
     gene            461..994
                     /locus_tag="ACZPB6_05495"
     CDS             461..994
                     /locus_tag="ACZPB6_05495"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR1183962.1"
                     /translation="MTTVNERAARIRMVVLDIDGVMSDGTINVSGTGELYKSFYVRDG
                     LGIKMLQKHGIEIAIITGRTSAIVAERMRELGVTRIAQGQRFKTTAYEALLEETGLTD
                     EQVAYMGDDVPDLPLLMRVGLSTTPADGNPEVLNYTHWQSRFPGGRGAVRELSELILK
                     SQGAWDTLVHNTYVLGK"
     gene            994..1620
                     /gene="lptC"
                     /locus_tag="ACZPB6_05500"
     CDS             994..1620
                     /gene="lptC"
                     /locus_tag="ACZPB6_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR1183963.1"
                     /translation="MIHRSLVLRERITAIVGAALLLLLVGASYYYSVQTRIEALKYVP
                     SETSPDFTARRVTLTDFDERGVATARLSANTMEHFSDERMRATDASYHSLDPAKPQLA
                     LTGDRAWSNDSLETVELEGNVQLSRAPFKDEPDLFFTTEYIKGYLDTYRFETDRKVYM
                     RRGIDTTEASNGMKYDNILHTVELDGSVVSVFHPNAQSTDTSANAAQK"
     gene            1617..2186
                     /gene="lptA"
                     /locus_tag="ACZPB6_05505"
     CDS             1617..2186
                     /gene="lptA"
                     /locus_tag="ACZPB6_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HIR1183964.1"
                     /translation="MKKSIVFSILAAIAAPTWALSTDKSQPIEVHADQFNGDEVKQTA
                     VYSGNVVVDQGSMNLTGARLDLAITPKGYRRATITGSPARFKQQRDPKTKGVEEWVHA
                     HASRITYDEETDKVTLEGKAQLSRSENGQQKDMTSGERIVYDMRNARSEVNSGEGKRV
                     TTIIAPRSKTDAGTQRQGTSLSNSTKLQQ"
     gene            2199..2972
                     /gene="lptB"
                     /locus_tag="ACZPB6_05510"
     CDS             2199..2972
                     /gene="lptB"
                     /locus_tag="ACZPB6_05510"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR1183965.1"
                     /translation="MTENATVSHSHTHALEATGLKKHYGPRNVVKGVSLAVKSGEVVG
                     LLGKNGAGKTTTFYMVVGLVVADDGQIRLDGQDITHMPIYKRARMGLSYLPQEASVFR
                     KLSVEDNVRAILELQEGEDGKPLSKDVIEERLDQLLEELQIQHLRTNQAISLSGGERR
                     RTEIARALAANPRFILLDEPFAGVDPIAVIEIQRIVRFLKERNIGVLITDHNVRETLS
                     ICDHAYIINEGEVLASGTPDELVNDQAVRKIYLGENFRM"
     gene            2977..4410
                     /gene="rpoN"
                     /locus_tag="ACZPB6_05515"
     CDS             2977..4410
                     /gene="rpoN"
                     /locus_tag="ACZPB6_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR1183966.1"
                     /translation="MENHLLATLSQQQVLSQEQQQSLRILQMPTQELLGEIELMLESN
                     PLLEVEEPAQASDDPQARAQEHDDDRRDDRGEDDSEFDAAQAETPVDQPYEAWQGASS
                     DEAPFERIPAPESFRDELLADLGCLPLTPRQHLLVACLVEELDDRGFFTSPFETLMRY
                     YARILKEEGVENVTAAEWREALATLQSMDPPGIGTAGPVEALMKQIERIEPSIMHAAA
                     LETLKRIVSKHLDKVAADNRPALLKLANGKSSILDEALRVLKTLSPYPIARESSTLYI
                     VPDVIVRTQNGVSTAHLNGSSQLRLRLRINDARSEADPSTRRVLLGEAKTFIQRIEAR
                     RATLQRLADAIVTRQQAYFEHGPAALRPMTLSQIAEELGLSVPTVSRAVSGKFIACSQ
                     GTVEMRSLFSAVSFSNQGSGAEEQGASEIQAMICKLIETEDPRAPLSDEMLCEMLQKQ
                     GVPIARRTVAKYRTCAGIPSARSRRRL"
     gene            4481..4987
                     /locus_tag="ACZPB6_05520"
     CDS             4481..4987
                     /locus_tag="ACZPB6_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR1183967.1"
                     /translation="MNLLAPLLTLETVRLNLDVVSRKRLYEEAGLVFETSAGLSHTEA
                     FDALFAREKLGSTCLGSGCALPHGRVEGITEPAAVFLRTAAPLSLDAPDGRPVQLFLC
                     LLIPENDDGMYLKILREAACLFGNKPLRNALLHAESEVKICELIHNWTPPADLHYEPD
                     FSEDDEDA"
     gene            5017..5919
                     /gene="rapZ"
                     /locus_tag="ACZPB6_05525"
     CDS             5017..5919
                     /gene="rapZ"
                     /locus_tag="ACZPB6_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR1183968.1"
                     /translation="MQLIIVTGMSGGGKSIAIRQLEDCGYYCIDNLPAAFLLPVAQDL
                     RTHGIKSAAVAIDARSQSTFDGSLKAIRQLEEAGFDVRILFLTASDQELVKRFSETRR
                     RHPLSTLAASRDQLVTLQEAISRERDLLAPISAGAALMDTTGILSSQLREWVKKFVGQ
                     PQAMMTLTFESFAFKHGLPVASDLVFDVRCLPNPYYDPALRPLTGRDKPVADYLRTFP
                     TVINMVDDIEGFIRKWLPAYIAQNRHYLTVAVGCTGGQHRSVFVAEELGARFRDTDGV
                     VVRHRIIEQKALLSGEKSVQNEGS"
     gene            complement(5929..7005)
                     /locus_tag="ACZPB6_05530"
     CDS             complement(5929..7005)
                     /locus_tag="ACZPB6_05530"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF012933.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR1183969.1"
                     /translation="MRDPEKLFADAIIGWHEACGRHDLPWQVEDWYPRLVSEVMLQQT
                     QVVSVLPKYEAFMTAFPTPECLAAAPDDDVMALWAGLGYYSRARNLLKAVRIIVNEMG
                     GRCPETAAELEKLPGLGPSTAGAVAAFAFRERAVMADGNAQRVLARVFMVEGNAAQPG
                     FVKEIRTLAASLLPSSELMPTYTQALMDFGAMLCKRNPDCGVCPLADCCGARLAGRVA
                     DFPGRKPRRERPVRHAVMVFAFAENGLWLRRRTEKGVWHGLWTPPLRQQDEPVESADP
                     LSVCPELEGGILRTIRLEPLVHDFTHYRLIIHSVAVDLAQGFAPEGFEFLGEKETASV
                     GMPTPVVKLLTICREERDFRPLLF"
     gene            7111..>7534
                     /locus_tag="ACZPB6_05535"
     CDS             7111..>7534
                     /locus_tag="ACZPB6_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183970.1"
                     /translation="MLAVFAAAAGCAAAFPGVDAHSSESRDAAQSLYEVIVAEIAAHR
                     EQPEVALALLDQTLARTKSSEVGELAWRTALQTRNPDIVLEQARAWAAIDPKAGLARR
                     TILLNAVEKGRQSEYVELLSRMYADAEDKAELITDVTTL"
BASE COUNT         1495 a         2718 c         2112 g         1209 t
ORIGIN      
        1 acgaaggagg acttcgcgcg cagccatccg ggcggagccc tcggccgccg cctgctcatc
       61 cacgttcagg acatcatgcg tacgggcgag gacattcccg ctgtccgcga ggaggtcggc
      121 gtgcttgagg cggtccgcga aatcacgaag aagcacatcg gcatgaccgc cgtcgtcgac
      181 gaccaaggcc acgtgaccgg catcttcacc gaaggcgacc tgcgccgcct catcgagcgt
      241 ctcggcgaca tccgtccgtg cgtcatgaag gacgtgatga tccgcaatcc gaagaccatc
      301 cgcgcccaag agctcgccgc ctccgccgtc aagctcctcg acaccttcca ctgcaaccag
      361 cttctcgtcg tcgacgagga caacaggctc atcggcgccc tgcacctgca cgacctgatg
      421 ggtgccaagg tcctttgatc cacttttcgg gagacccgac atgaccacag tgaacgagcg
      481 cgcggcccgc atcagaatgg tcgtactcga catcgacggc gtgatgagcg acggcacgat
      541 caacgtctcg ggcacgggcg agctctacaa gagcttctac gtgcgcgacg gactcggcat
      601 caagatgctg cagaagcacg gcatcgagat cgcgatcatc acgggccgca cctctgcgat
      661 cgtcgccgag cgcatgaggg agctcggcgt cacccgcatc gcccagggcc agcgcttcaa
      721 gaccaccgcc tacgaggcac ttcttgagga gaccggtctc accgacgagc aggtcgccta
      781 catgggcgac gacgtccccg acctgccgct tctgatgcga gtgggcctct ccacgactcc
      841 cgcagacggg aatccggagg ttttgaacta tactcactgg cagtcccgct tccccggcgg
      901 ccgcggcgcc gtcagggagt tgtccgaact catcctcaaa tcccagggcg catgggatac
      961 actcgtgcac aacacctacg tcctcggcaa ataatgatcc accgcagcct agtcctccgc
     1021 gaacgcatca ccgccatcgt cggcgcggcc ctgctgttgc tgctcgtcgg cgcaagctac
     1081 tactactcgg tccagacccg catcgaggcc ctgaagtacg tccccagcga aacgagcccc
     1141 gacttcacgg cgcgccgcgt cacgctgacc gacttcgacg aacgcggcgt cgcaaccgca
     1201 cgcctctccg ccaacacgat ggagcacttc tcagacgagc gcatgcgcgc cacggacgcc
     1261 agctatcact ccctcgatcc tgcgaagccc cagctcgccc tcacgggcga ccgcgcatgg
     1321 agcaacgaca gcctcgagac cgtcgagctt gaaggcaacg tgcagctgag ccgcgcaccc
     1381 ttcaaggacg agcccgatct ctttttcacg accgaataca tcaaggggta tctcgacacc
     1441 tacaggttcg agacggacag gaaggtctac atgcgccgcg gcatcgacac caccgaagcc
     1501 tccaacggca tgaagtacga caacatcctg cacacggtcg agctcgacgg cagcgtcgtc
     1561 tcggtcttcc atcccaacgc gcagtccact gacacatccg ccaacgcagc ccaaaaatga
     1621 agaagtcaat cgttttcagc atcctcgccg ccatcgcagc gccgacctgg gccctgagca
     1681 ccgacaagag ccagcccatt gaagtccatg ccgaccaatt caacggtgac gaagtcaagc
     1741 agaccgccgt ctactccggc aacgtcgtcg tcgatcaggg ctcgatgaac ctgacgggcg
     1801 ctcgcctcga tcttgcaatc acgcccaagg gctaccgtcg cgccacgatc acgggctctc
     1861 ccgcacgctt caagcagcag cgcgatccga agacgaaggg cgttgaggaa tgggtgcacg
     1921 cgcatgcgag ccgcatcacc tacgacgagg agaccgacaa ggtcacgctc gaaggcaagg
     1981 cccagctctc gcgcagcgaa aacggccagc agaaggacat gacgagcggc gagcgcatcg
     2041 tctacgacat gcgcaacgcc cgttccgaag tcaacagcgg cgaaggcaag cgcgtcacga
     2101 cgatcatcgc gccccgctcc aagaccgacg cgggcacgca gcgtcagggc acgtccctct
     2161 ccaactccac caagcttcag cagtaacagg acttcagcgt gaccgaaaac gcaacagtta
     2221 gccattccca cacccacgcg ctcgaggcga ccggcctcaa gaagcactac gggccccgaa
     2281 acgtcgtcaa gggcgtcagc ctcgccgtca agtcgggcga ggtcgtggga ctgctcggca
     2341 agaacggtgc aggcaagacg acgaccttct acatggtcgt gggccttgtc gtggccgacg
     2401 acggccagat ccgcctcgac ggtcaggaca tcacgcacat gcccatctac aagcgtgcgc
     2461 gcatggggct ctcctatctg ccccaggagg caagcgtctt ccgcaagctc tccgtcgagg
     2521 acaacgtccg cgccattctc gagcttcagg agggagagga cggcaagccg ctttccaagg
     2581 acgtgatcga ggagcggctc gaccagctgc tcgaagagct gcagatccag caccttcgca
     2641 cgaaccaggc catttcgctc tcgggcggcg agcgccgccg cacggaaatc gcccgcgccc
     2701 ttgccgccaa tccccgcttc attctgctcg acgagccctt cgcgggcgtc gacccgatcg
     2761 ccgtgatcga gattcagcgc atcgtgcgct tcctcaagga gcgcaacatc ggcgttctga
     2821 tcaccgacca caacgtccgc gaaaccctgt cgatctgcga ccacgcctac atcatcaacg
     2881 aaggcgaagt gctcgcctcg ggcacgcccg acgagctcgt caacgaccag gccgtccgca
     2941 agatctatct cggcgaaaac ttccggatgt agccaaatgg aaaatcatct cctcgccaca
     3001 ctctcccagc agcaggttct ctcccaggag cagcagcagt cgctgcgcat tctgcagatg
     3061 cccacgcagg agcttctcgg cgaaatcgaa ctcatgctcg aatccaaccc cctgcttgaa
     3121 gtggaggagc ctgcacaggc ctccgacgac ccgcaggcgc gcgctcagga gcatgacgac
     3181 gacaggcggg acgaccgcgg cgaggatgat tccgaattcg acgcagccca ggcggagacg
     3241 cccgtcgacc agccctacga ggcctggcag ggcgcctcca gcgacgaagc gcccttcgag
     3301 cgcattcccg cccccgaatc cttcagggac gagctgctcg ccgatctcgg ctgcctgccg
     3361 ctcacgccgc gccagcatct gctcgtcgcc tgccttgtcg aggagctcga cgacaggggc
     3421 ttcttcacgt ccccgttcga gacgctcatg cgctactacg cgcgcatcct caaggaggaa
     3481 ggcgtcgaga acgtgaccgc cgccgagtgg cgcgaagccc tcgccaccct tcagagcatg
     3541 gatcctccgg gcatcggcac cgccggcccc gtcgaagccc tcatgaagca gatcgagcgc
     3601 atcgagccct ccatcatgca tgccgccgcg cttgaaaccc tcaagcgcat cgtctccaaa
     3661 catctcgaca aggtggccgc cgacaaccgt cctgcgcttc tcaagctcgc caacggcaag
     3721 tcctccattc tcgacgaggc gctgcgcgtg ctcaagaccc tctcgcccta tccgatcgcc
     3781 agagaatcca gcacgctcta catcgttccc gatgtcatcg tcaggacgca gaacggcgtg
     3841 agcacagcgc acctcaacgg ctcctcgcag ctgcgccttc gccttcgcat caacgacgcc
     3901 aggagcgagg ccgatccctc gacccgccgc gtccttctcg gcgaagccaa gaccttcatt
     3961 cagcgcatcg aggccagacg agccaccctg cagcgtctgg ccgacgccat cgtcacgcgc
     4021 cagcaggcct acttcgagca cggccccgcc gccctgcgtc cgatgacgct cagccagatc
     4081 gccgaagaac tcggcctgtc cgttcccacg gtctcccgcg ccgtttccgg caagttcatc
     4141 gcctgcagcc agggcacggt cgagatgcgc tcgctcttca gcgccgtctc cttcagcaac
     4201 cagggcagcg gcgccgagga gcagggagcc tccgaaatcc aggccatgat ctgcaagctc
     4261 atcgagaccg aggaccccag agcccccctt tcggacgaaa tgctctgcga aatgcttcag
     4321 aagcagggtg tgcccatcgc ccgccgcacg gtcgccaagt accgcacctg cgcaggcatt
     4381 ccctcggctc gctcccgccg caggctctga cttctccacc cctttggtac actgaacgaa
     4441 gttttcgatt cgacggtccc acaaggccgt ccgacgcgtc atgaatcttc tcgcacctct
     4501 gcttactctt gaaaccgttc gccttaacct cgacgtcgtg agccgcaagc gcctctacga
     4561 agaggcgggc ctcgtcttcg aaacctccgc tggtctttcc cacacggagg ccttcgacgc
     4621 gctcttcgca cgcgaaaaac tcggttccac ctgcctcggc agcggctgcg cgcttcccca
     4681 cggccgcgtc gagggcatca ccgaacccgc cgccgtcttt ctgaggaccg ccgcgccact
     4741 ctcccttgat gcgcccgacg gccgtcccgt ccagctgttc ctctgcctgc tcattcccga
     4801 aaacgacgac ggcatgtacc tgaagatcct gcgcgaagcc gcctgcctct tcggcaacaa
     4861 gccgctgaga aacgcgctgc ttcacgcgga gtcggaggtc aagatctgtg aactcattca
     4921 caactggacg cctcccgccg accttcacta cgagcccgac ttctccgaag acgacgaaga
     4981 cgcctgaaaa aactacgcca atacggaggc aacgccatgc aactcatcat cgtgaccggc
     5041 atgtccggcg gcggcaagtc catcgccatc cgccagcttg aggactgcgg ctactactgc
     5101 atcgacaacc tgcccgccgc cttcctgctc cccgtggcgc aggacctccg cacgcacggc
     5161 atcaagtccg ccgccgtcgc catcgacgcg cgaagccaga gcaccttcga cggctccctc
     5221 aaggcaatcc gacaactcga ggaggcgggc ttcgacgtgc gcatcctctt tctcaccgcc
     5281 tcggatcagg agctcgtcaa gcgcttctcc gaaaccaggc gccgccatcc gctctcgacg
     5341 ctcgccgcca gccgcgacca gctcgtcacg cttcaggagg ccatcagccg cgaacgcgac
     5401 ctgctcgccc ccatttctgc aggcgcggcc ctgatggaca ccaccggcat tctttcctcg
     5461 cagcttcgcg aatgggtgaa gaagttcgtc ggccagcccc aggccatgat gacgctcacg
     5521 ttcgagtcct tcgccttcaa gcacggcctt cccgtcgcca gcgatctggt cttcgacgtg
     5581 cgctgcctgc cgaaccccta ctacgatcct gcgctgcgcc ccctgacggg acgggacaag
     5641 cccgtcgccg actaccttcg caccttcccg accgtcatca acatggttga cgacattgaa
     5701 ggcttcatcc gcaagtggct tcccgcctac attgctcaga accgccacta tctgaccgtc
     5761 gccgtcggct gcacgggcgg acagcaccgc tccgtcttcg tggccgaaga gctcggcgcc
     5821 cgcttcaggg acacggacgg cgtcgtcgtg cgacaccgca tcatcgagca gaaggccctg
     5881 ctgtcgggcg agaagtccgt ccagaacgaa ggcagctgat tcagaagatc agaaaagcag
     5941 gggacggaag tcccgctcct ccctgcagat cgtcagcagc ttcaccaccg gcgtcggcat
     6001 tcccaccgac gccgtttcct tttctccgag aaactcgaag ccctcggggg cgaacccctg
     6061 tgccagatcg acggccacgg aatgaatgat gaggcggtaa tgcgtgaagt cgtgcacgag
     6121 aggctcgagc ctgatcgtgc ggagaattcc gccctcaagt tcaggacaga cggaaagcgg
     6181 atccgccgac tccacgggct cgtcctgctg cctcaggggc ggcgtccaga ggccgtgcca
     6241 gacgcccttt tccgtgcggc gcctgagcca gaggccgttc tcggcaaacg cgaacaccat
     6301 caccgcatgc ctcacgggac gctcgcggcg gggcttgcga ccgggaaagt cggccaccct
     6361 gcccgccaga cgcgcgccgc agcagtccgc cagaggacag accccgcagt cgggattccg
     6421 cttgcagagc atggcgccga agtccatcag cgcctgagta tatgtcggca taagctccga
     6481 agaaggcagc agggaggcgg cgagcgtcct gatctccttc acgaagccgg gctgtgcggc
     6541 gttgccctcg accatgaaca cgcgcgcgag cacgcgctgt gcgttcccgt ccgccatgac
     6601 ggccctctcg cggaaggcga aagcggccac ggcgcccgcc gtcgaggggc cgagtcccgg
     6661 aagtttttca agctcggccg cggtttcagg gcatctcccg cccatttcgt tgacgatgat
     6721 ccggaccgcc ttgagcaggt tgcgggcgcg cgagtagtat ccgaggcccg cccagagcgc
     6781 catgacgtcg tcgtcgggcg cagccgccag acactcgggc gtcggaaagg cggtcatgaa
     6841 ggcctcgtac ttgggaagca ccgacacgac ctgcgtctgc tgcagcatca cctccgagac
     6901 aagtctcgga taccagtcct cgacctgcca cggcaggtcg tgcctgccgc aggcttcgtg
     6961 ccagccgatg attgcatctg caaataactt ctcgggatcc cgcatgccgc attcctttag
     7021 ttttcgaggt aaagtacgga gacactctac accaacggct tcccaagctt tcactctttc
     7081 atgaccggaa aactattcaa aaaagcggcc gtgctcgccg tcttcgccgc agccgcaggc
     7141 tgcgcggcgg ccttccccgg cgtggacgcg cattcctcag aatcgcgcga tgccgcccag
     7201 tctctttatg aagtcatagt cgccgagatc gccgcgcaca gggagcagcc cgaggttgct
     7261 ctggcgctcc tcgaccagac gctcgcgcga acgaagtcct cggaagtggg cgaactcgcc
     7321 tggcgcaccg cccttcagac ccgcaatccc gacatcgtgc ttgaacaggc gcgcgcctgg
     7381 gccgcgatcg atccgaaagc tggccttgcc cggcgcacga ttctcctcaa cgccgtcgaa
     7441 aagggccgtc agtccgaata cgtcgagctg ctctcccgca tgtacgcaga cgcggaggac
     7501 aaggccgagc tgatcacgga cgtcacgacg ctcc
//
LOCUS       DBMDTI010000088         7449 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2642_length_7449_cov_3.368400, whole genome shotgun sequence.
ACCESSION   DBMDTI010000088 DBMDTI010000000
VERSION     DBMDTI010000088.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2642_length_7449_cov_3.368400"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(8..814)
                     /locus_tag="ACZPB6_05540"
     CDS             complement(8..814)
                     /locus_tag="ACZPB6_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013757.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR1183971.1"
                     /translation="MTESSESRHGIQVLDRANRLLARLEASSEPLALTTLARETGLSA
                     STAHRILGSLSAMGFVKQNPAGQWSLGLRLLELGNLVYERFTVRQRALPFMQQLHAAT
                     GMTVNLAIRDNDRLLYIEHVLAPEYKANQLRRIGSWAPLHITSGGKLFLARMSPDEIR
                     GYTERTGLKAGTQNSIQSLDHLLVTLAHARETGWAYDREEYESNVHCVAAPIFGAGGR
                     LIATISLAAHRSNEFTPERIAKLCGIAQSISSAMGAGIGERIKGKGYGDG"
     gene            complement(811..2826)
                     /locus_tag="ACZPB6_05545"
     CDS             complement(811..2826)
                     /locus_tag="ACZPB6_05545"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR1183972.1"
                     /translation="MTDHPIRPAFWNVPLWGEIGVYVLGFTAVALLIAGILRNRRLWL
                     RGAEDPHPVKDRRARLFSVIADVFTQKKIRETSAGRLHAVLAWGFFLLFCGTALATLD
                     WDVGHYVFDAQFLQGGFYLAYKFVLDCAGIAVLIALALGAARRWSKESGLPGDARFVC
                     AYLSLAFIVLTGFLVEGIRLAATLPAWSVYSPIGHLVAKMLLACGLSEAELRLLHTWI
                     WTIHGISSLAFIAAVPLTYYAHVYRTPAGLFTREEKPAGLLRKIDDIEEQETFGVSSF
                     SQFTTRERTAFDACTECGRCTAACPAVRAGTPLDPRALIVSLRDRMHMEEKTEEAAAL
                     PSLEETVSRDALWSCTTCGACSRACPAGISLPEIIVNMRRHLALEAGDFPEGVANALE
                     NTASVGNPWGLDPYERLDWAKDLDVPVAESGVHYDVLYWVGCAASYDRRARKIARSMV
                     RILKATGVNFAVMAEERCHGEFARRLGEEYLYQTAAQENIGNFAQYDFDRILTACPHC
                     FNTLKNEYPSFEDFRWPVVSHAVFIDELMKAGSLPYVKSEGLSAVYHDPCYLARINGI
                     VAEPRSVLNSVCTVLKSPAESGERTLCCGAGGGQMWIDRHGSNSVNVIRLKDLRASGA
                     DTIAVSCPHCLTMLESARAVTRDAESVSISDIAELVAAALPEEDASK"
     gene            complement(2927..3436)
                     /gene="thpR"
                     /locus_tag="ACZPB6_05550"
     CDS             complement(2927..3436)
                     /gene="thpR"
                     /locus_tag="ACZPB6_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR1183973.1"
                     /translation="MPRVFAALLLPHDVCDALARDAKAFARIDPLARFTRSTNLHLTL
                     AFVGEISTIDARRLSAALRPLCALTPRDITIGRIGTFDRQRILYAGLSPAESLDPVVN
                     EVRSTITRLGLPLDDKPFRAHITLARDWRRGHPPMTLPARTVPLSGPVLMETVKDPRT
                     GMIRYRQVL"
     gene            complement(3525..4577)
                     /locus_tag="ACZPB6_05555"
     CDS             complement(3525..4577)
                     /locus_tag="ACZPB6_05555"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIR1183974.1"
                     /translation="MLYSLCRRVLFSMQPETAHNVTMANLDWATHLGLHKLLTHMPDE
                     DPVEVMGLRFPNTIGLAAGMDKDGIRVNAFGALGFGHVEIGTITPLAQPGNPKPRCFR
                     VIPAKGIINRMGFNNEGCDKVIENLRSADAFRLRGGILGINIGKNAVTPIENALSDYE
                     KCLDKVYDHADYIAVNISSPNTKNLRQLQGADKFDHLVSGIVTRRQQLKDARNGKHVP
                     IAVKLAPDLENEEILRCVDTLIERGIDAVIATNTTITRDRIQGMPNCTETGGLSGAPL
                     RERSTEVVRLIADHVQGALPIIASGGVMTPQDAVEKMEAGAKLLQLYTGFIYSGPALI
                     AECSEAVARWRAAQKA"
     gene            complement(4584..6212)
                     /locus_tag="ACZPB6_05560"
     CDS             complement(4584..6212)
                     /locus_tag="ACZPB6_05560"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF002346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HIR1183975.1"
                     /translation="MTTDSAQDDIPIISGDAVGFDMRPRIPEDEERFTFSTVCRSNGL
                     SSRLIISGYTQGSFPWPDAKDADAGLYPWGRLFPCTILKTGRVNLTHSMRRRVRDAVH
                     RHYSGLELEILLDYDFDEVIEAIAEYHRKRSNGTWMTRDMIDMWKALHRQRIAHCVSV
                     HIDGELAGGLYFTSVGRMLYGESMFSLRPDASKLALAALAAFAGMAHLPVIDCQMPTE
                     HVIRMGAAVVEGRAFLKLNEFLARQSHFPWSRSESLSLLPFLESVYSEYAERTPNDLT
                     IVHEQRFAHAGGMLFHTLAIDGTCNYYEDGRRSTMEVIPLASTDPRVTPMYNRLIEAG
                     FRRDSTYLYRLACNECRRCIPTRIDVHAFRPDDTMRRTMKRNASLVMRELKLAPMTEE
                     QYELYKRYQDSRHRRSVMADMDRSTVSTVLFSTCSDTRLLEFRTSPDAPRPNELKMVC
                     IIDCLDNALSAVYTFFDPDVPKLSLGTFGILSEIEYARRNGLSYVYLGYWLPDYPGMD
                     YKTRFQPMDIFWENGWMPERKFTADPKLLDPTSL"
     gene            6349..6425
                     /locus_tag="ACZPB6_05565"
     tRNA            6349..6425
                     /locus_tag="ACZPB6_05565"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6383..6385,aa:Met,seq:cat)
     gene            complement(6501..7319)
                     /locus_tag="ACZPB6_05570"
     CDS             complement(6501..7319)
                     /locus_tag="ACZPB6_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1183976.1"
                     /translation="MTASAIRSATGCATPLTRLRRALPPVLLPILALGTFASIDALQT
                     QMRLPEHALFMSTGDTVELTGVIRAPLPSAPPARRLTISPDSVPVTLSGVTTSQRTLS
                     DDTVWRAKLTLGEVPAHLAFKADISFPDLHHEASQSWQIEAWPDRTSMQEASPSLLVS
                     KLGIDPLPAAFACLISALLLALLYPALYFIDRRTLARSGCLRVFHARTSGPDTLLYCV
                     QPVRDAPVRGTAYRVLSATGQLLGMAVLADSGRRHCVFRLHAARARAGCIVAVK"
BASE COUNT         1487 a         1912 c         2593 g         1457 t
ORIGIN      
        1 attggggtta gccgtcaccg tacccctttc ccttgatgcg ctctccgatc cctgcgccca
       61 tggcggagga tatcgactgg gcgatgccgc agagcttggc gatccgctcg ggggtgaact
      121 cgttggaacg atgggcggcg agcgagatcg tggcgatcag cctgccgccg gcaccgaaga
      181 tcggtgcggc aacgcagtgc acgtttgact cgtactcctc gcggtcataa gcccagccgg
      241 tttcacgggc gtgcgcaagg gtcacgagca ggtggtcgag ggactgaatg gagttctgcg
      301 tccctgcctt caggcctgtt ctttccgtgt agccgcgaat ttcgtcaggt gacatgcgtg
      361 ccagaaagag cttgccgcct gacgtgatgt gcagaggtgc ccaagacccg atgcggcgaa
      421 gttggttggc cttgtattca ggcgcaagca catgttcgat gtagagcagc ctgtcgttgt
      481 cgcgaatggc cagattcacc gtcatgcctg ttgcggcgtg aagctgctgc atgaacggaa
      541 gagcgcgctg gcgcaccgtg aagcgctcgt agacgaggtt gccgagttcc aacagcctga
      601 gtccaagact ccactgtccg gcgggattct gcttgacgaa tcccatggcc gagagagacc
      661 ccagaattcg gtgggccgtc gaggctgaaa gtcccgtttc ccgggcgagc gttgtcaggg
      721 cgagcggttc ggacgaggcc tccagtctgg caagcagacg gttcgctcgg tcaagcacct
      781 gaatgccgtg tctggattcg gagctctccg tcatttggag gcgtcctctt caggaagcgc
      841 ggcggcgacg agttcggcga tgtcggagat gctgaccgac tcagcatccc gggtaacggc
      901 tcgggcgctc tcgagcatcg tgagacagtg cgggcaggat acggcgatgg tgtctgctcc
      961 cgaggcgcgg aggtccttca ggcggatgac gttgacggag ttggagccgt gcctgtcgat
     1021 ccacatctgc ccaccgcccg caccgcagca gagcgtgcgc tcgccggatt cggcggggga
     1081 cttgaggacg gtacagacgg agttgagcac gctgcggggc tctgcgacga tgccgttgat
     1141 gcgcgcgagg tagcagggat cgtggtagac cgcactgagc ccttcggact tgacgtacgg
     1201 aagactgcct gccttcatga gttcgtcaat gaaaacagcg tgactgacca cgggccagcg
     1261 gaagtcctcg aaggacggat attcattctt gagcgtattg aagcagtggg ggcaggccgt
     1321 caggatgcgg tcgaagtcat attgggcgaa gtttccgatg ttttcctgag cagcggtctg
     1381 gtagaggtat tcctcgccga gtctgcgggc gaactcgccg tggcagcgct cttcggccat
     1441 gacggcgaag ttgacccctg tggccttgag aatcctcacc atgctgcgtg cgatcttgcg
     1501 agcgcggcgg tcgtagctgg cggcgcagcc aacccagtag aggacgtcgt agtgaacacc
     1561 gctttctgcg accggaacat caagatcctt ggcccaatcg aggcgttcgt agggatcaag
     1621 tccccaggga ttgccgacgg aggcggtgtt ttcgagcgca ttggcgacgc cttcagggaa
     1681 gtcgcctgct tcgagcgcca gatggcggcg catgttgacg atgatttcgg ggagactgat
     1741 gccggcaggg caggcgcgtg agcaggcgcc gcaggttgtg caggaccaga gggcgtcgcg
     1801 ggagacggtt tcctcgagag acggaagcgc ggctgcttct tcggtcttct cctccatgtg
     1861 catgcggtca cgcagtgaaa cgatcagggc tcgaggatca agcggcgttc ctgcgcggac
     1921 cgcaggacag gcggccgtgc aacggccgca ttccgtgcag gcgtcgaagg ccgttcgctc
     1981 gcgggtcgtg aactgactga agctggagac gccgaacgtt tcctgctcct caatgtcgtc
     2041 gatcttgcga aggaggcctg cgggcttttc ctcacgggtg aagagccctg caggtgtgcg
     2101 gtagacgtgc gcgtaataag taagcggaac ggctgcaatg aaggcaaggg atgagatgcc
     2161 gtgaatcgtc cagatccagg tgtgaagcag acgtagttcc gcctccgaca gaccgcaggc
     2221 gaggagcatc ttggcaacga gatgcccgat gggcgaatac acggaccagg cgggcagggt
     2281 ggccgccaga cggatgcctt caacaaggaa gcctgtgagc acgatgaatg cgagcgagag
     2341 ataggcgcag acaaatcgtg cgtcaccggg caggcccgac tccttggacc agcggcgggc
     2401 ggctccgagc gcgagtgcga tcagaacggc gatgcctgcg cagtcgagca cgaacttgta
     2461 ggcgaggtag aagcctccct gaaggaactg agcatcaaag acgtagtggc ctacgtccca
     2521 gtcgagcgtg gcgagcgccg tgccgcaaaa gagcaggaag aagccccagg caagcactgc
     2581 gtgtaggcgc cctgcgctcg tctcgcggat cttcttctga gtgaagacgt cggcgatgac
     2641 ggagaaaaga cgggctcttc tatccttgac ggggtgtgga tcctctgcgc ctcgaagcca
     2701 caggcggcga ttgcgtagga tgcctgcgat gaggagcgcg acggcggtga agccgagcac
     2761 atagacgccg atttcgcccc aaaggggcac gttccagaag gcggggcgaa tgggatggtc
     2821 tgtcatggga gactgtggta cggggttgat gaatgagggc gtcattgtac taaaaaagaa
     2881 gggagacttt ccgcaatgcg gaaagtctcc cttcgatggc tgtttctcag aggacctgac
     2941 ggtagcggat catgcccgtg cgtggatcct tgacggtttc catcagaacg gggcctgaga
     3001 gcgggactgt gcgggcggga agcgtcatgg gcggatggcc gcggcgccag tcgcgggcaa
     3061 gcgtgatgtg ggctctgaag ggcttgtcgt cgagcggaag gccgagtcgg gtgatggtac
     3121 tcctgacttc gttgacgacg ggatcgaggc tctcggcggg ggagaggccc gcgtagagaa
     3181 tgcgctgtcg atcgaacgta ccgatgcgtc cgatcgtgat gtcgcgcggc gtaagcgcac
     3241 agagcggacg gagggcggcg gagagtctgc gggcgtcgat cgtcgaaatc tcgcccacaa
     3301 aggcaagcgt caggtgaagg ttggtgcttc gggtgaagcg ggccagagga tcgattctgg
     3361 cgaaagcctt ggcgtcgcgg gcaagagcgt cgcacacgtc gtgaggcagc agaagcgcgg
     3421 caaaaacgcg gggcatggca aatcctgtca ttgaagggaa aaagaaaagg gcgctgcggt
     3481 ccgaggccgc agcgcccatt tgttctcaag gaagaccttg ttgattaggc cttctgagcg
     3541 gctcgccaac gggcgaccgc ttcggaacat tcggcgatga gggccggacc ggaatagatg
     3601 aagccggtgt aaagctggag gagcttggcg cccgcttcca tcttttcaac ggcatcctga
     3661 ggcgtcatga cgccgccgga ggcgatgatc ggaagagcac cctgaacgtg atcggcgatg
     3721 aggcggacga cttcagtgga gcgttcgcgg agcggagcgc cggaaaggcc gcccgtttcc
     3781 gtgcagttgg gcatgccctg aatgcggtcg cgcgtgatgg tcgtgttggt ggcgatcacg
     3841 gcgtcgatgc cgcgctcgat gagcgtgtcg acgcaacgca ggatctcttc gttttcaaga
     3901 tcgggggcaa gcttgacagc gatgggcacg tgcttgccgt tgcgggcgtc cttgagttgc
     3961 tggcggcggg ttacgatgcc ggaaacaaga tggtcgaact tgtcggcgcc ctgaagctgg
     4021 cgaagattct tcgtgttggg cgaggagatg ttgacggcga tgtagtcggc gtggtcgtag
     4081 accttgtcga ggcacttttc atagtcggaa agcgcatttt cgatcggcgt gacggcgttc
     4141 ttgccgatgt tgatgccgag aatgccgccg cgcagacgga aggcgtcggc gctgcggagg
     4201 ttttcgatca ccttgtcgca gccttcgttg ttgaagccca tgcggttgat gatgcccttg
     4261 gccgggatga cgcggaagca gcggggcttc ggattgccgg gctgggcgag cggcgtgatc
     4321 gtgccgattt cgacgtggcc gaagccgagg gcgccgaagg cgttcacgcg gatgccgtcc
     4381 ttgtccatgc cggcggcaag accgatcgtg ttggggaagc gcaggcccat cacttcaacc
     4441 ggatcctcgt caggcatgtg cgtcaggagc ttgtgaagtc cgagatgcgt ggcccagtcg
     4501 aggttggcca tggtgacgtt gtgagcggtt tccggctgca tcgaaaacag cacgcggcgg
     4561 caaagcgagt aaagcatgat gatctaaagg gacgttgggt cgagaagctt ggggtcggct
     4621 gtaaattttc gttccggcat ccagccgttc tcccagaata tgtccatcgg ctggaagcgc
     4681 gtcttgtagt ccatgcctgg atagtcggga agccagtacc ccagatacac gtaactgagg
     4741 ccgtttcgac gggcatattc gatttcggag aggatgccga aagtcccgag actcagcttg
     4801 ggaacatcgg ggtcgaagaa cgtatagacg gcactgagtg cgttgtctaa acaatctatt
     4861 atacaaacca tcttaagctc gttggggcga ggggcatcag gggaagtccg aaactcaagg
     4921 aggcgggtgt ccgagcacgt ggagaagagc accgtgctta ccgtgctgcg gtccatgtcc
     4981 gccatgacgc tgcggcgatg gcgcgagtcc tgatagcgct tgtagagttc gtactgttcc
     5041 tccgtcatgg gggccagctt gagctcgcgc atgacgagtg atgcattgcg cttcatcgtg
     5101 cggcgcattg tgtcgtcagg cctgaaggcg tgaacgtcga tgcgagtcgg gatgcaccga
     5161 cggcattcgt tgcaggcaag tctgtacagg taagtgctgt cgcgtcgaaa gccggcctcg
     5221 atcagacggt tgtacatggg cgtgacgcgg ggatcggtgg aagcaagtgg aatgacttcc
     5281 atcgtggatc tgcgaccgtc ctcatagtag ttgcacgtgc cgtcgatggc gagcgtatgg
     5341 aaaagcattc cacccgcgtg tgcgaagcgc tgttcatgga cgatggtcag atcgttcggc
     5401 gtgcgttcgg cgtattcgga atagacggac tcaagaaaag gaagcaggct gaggctttcc
     5461 gagcgactcc aggggaaatg ggattgcctc gcaagaaact cgttgagttt gagaaaggcc
     5521 ctgccttcaa cgacggcggc tcccatgcgg atgacgtgtt cggtgggcat ctggcagtcg
     5581 atgacgggca ggtgcgccat gcctgcaaag gctgccagtg cggcgagagc aagcttggat
     5641 gcgtctggac gaagggagaa catgctttcg ccgtagagca tccgtccgac ggatgtgaag
     5701 tagaggccgc ctgcgagctc tccgtcgatg tggacggata cgcagtgggc gatcctttgt
     5761 ctgtggaggg ccttccacat gtcaatcatg tcccgagtca tccaggtgcc gttcgagcgc
     5821 ttcctgtgat attcggcaat ggcctcaatc acctcgtcaa agtcgtagtc gaggagaatt
     5881 tcaagctcaa gtcccgaata gtgtcgatgg acggcgtcac ggacgcgcct tctcatggaa
     5941 tgagtgaggt tgacgcgtcc tgtcttaaga atggtgcacg gaaagagtcg cccccaggga
     6001 tagagtcccg cgtccgcatc ctttgcgtca ggccagggga aggaaccctg cgtgtaacct
     6061 gatatgatga gacgggaaga aaggccgttg ctgcggcata cggtggagaa tgtgaagcgc
     6121 tcctcgtctt cgggaatgcg gggcctcatg tcgaagccga cggcgtcgcc gctgatgatg
     6181 gggatgtcgt cctgggcgga atctgttgtc atggacttgc gttcgtgatg aaggtcaggc
     6241 tgtcatcata gcccagtcaa aaaatatttt tgccgaactc ttgacaaacg aaaaaatagt
     6301 ctgtaatatt caaatcctca acggaacagt ccgtttcgac agaattgccg cggggtggag
     6361 cagcccggta gctcgtcggg ctcataaccc gaaggtcgga ggttcaaatc ctccccccgc
     6421 aaccaatatg aaggcctcgg ttcgaaggaa ccgaggcctt tttgttgccc taaattcatg
     6481 gggcgagatt ttcatccctg tcatttcacg gcgacaatgc agcctgcgcg ggctcgggcc
     6541 gcatgcagtc tgaatacgca gtgtcgtcgg cctgagtcag ctaggaccgc catgccgagc
     6601 agctgcccgg tggcggaaag cacccggtag gcagtgcctc gaacgggtgc atctcttaca
     6661 ggctgaacgc agtagaggag tgtgtcgggc cctgacgtgc gggcgtgaaa gacgcgaaga
     6721 cagccgctcc gggcgagtgt tctcctgtcg atgaagtaga gtgcggggta gagcaatgcc
     6781 agcaggagcg cagagatcag gcaggcgaag gccgcaggca ggggatcaat gcctagtttg
     6841 gagacgagca gagagggact ggcctcctgc atcgatgtgc gatctggcca ggcttcaatc
     6901 tgccaggatt gggaggcctc atgatggagg tcaggaaaac tgatgtctgc cttgaaggca
     6961 aggtgtgcag gtacctcgcc aagagtcagt ttggcacgcc agacggtgtc gtctgagagc
     7021 gttctttgac tggtcgtgac gccggagagt gtgacgggca cggagtcggg gctgatagtc
     7081 aggcgccggg cgggaggtgc ggacgggagt ggtgcgcgaa tgacgcctgt cagttcaacg
     7141 gtgtctcccg tactcatgaa gagggcgtgc tcgggaaggc gcatctgcgt ctgaagcgca
     7201 tcaatgctgg cgaacgtgcc cagtgcgagg attggaagaa gcacgggcgg aagggcgcgg
     7261 cgaagcctag tgaggggcgt cgcacagcct gtggcggagc gaatggcgga tgcggtcatg
     7321 ggttctacaa atgctggagc ttgggacgta ggtgtgatgc gtgagtcttt tgcaaaagcc
     7381 tgaggcgcaa gtctaatgcg aatgtctacg cttgattgta gggtagggca gacatgtcag
     7441 ccgagccag
//
LOCUS       DBMDTI010000089         7399 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2661_length_7399_cov_4.073034, whole genome shotgun sequence.
ACCESSION   DBMDTI010000089 DBMDTI010000000
VERSION     DBMDTI010000089.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7399)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2661_length_7399_cov_4.073034"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="ACZPB6_05575"
     CDS             <1..685
                     /locus_tag="ACZPB6_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0120010 - intermembrane phospholipid
                     transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR1183977.1"
                     /translation="HAFNVMVDDAALKPVTKGYRWVTPQFVRDGVSNAIDNLGEVNNT
                     LNNVLQGKVDQGITSAFRFMLNSTIGVFGLFDVATWVGMERAPEDFGQTLGVWGVPEG
                     PYFELPLLGPSGGRDIFKWPVQTATNPITYLTWDEWEWSTAYAVVDAVDTRSRMMDAG
                     LDDMRANVVDEYVAIRDAYRQSRRMAVADGQIDEDAQLNSLTPLSFDDEEAEDDAGKP
                     AETDKDKEK"
     gene            688..1329
                     /locus_tag="ACZPB6_05580"
     CDS             688..1329
                     /locus_tag="ACZPB6_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1183978.1"
                     /translation="MQRRTLVLGGACGLALAAMPALAAEAVDPFEWIVALNNSILDEI
                     RADPKLHSGDQTALQALVDRRVMAAADFAMMTRMTIGPKWRKATKEERQRLMDGFEKL
                     LIRVYSGALKTVTDHKCELRPTRNRTIKDEMVIRTILKSTGNPDIGIDFRIYRDKAGE
                     WKIVDVNVEGIWMVENYRSQFASTLNTEGIPGLIKLLEEKADGLVDANAHKVQ"
     gene            1333..1605
                     /locus_tag="ACZPB6_05585"
     CDS             1333..1605
                     /locus_tag="ACZPB6_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013868.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR1183979.1"
                     /translation="MKFGTESITFREAKKAKDDIAIAVRNGDFVLDFEGVHRIDSTVL
                     ALSLHMLREAKKAGSTLRILSAPRGFADLVKLYGLETLLMPALEEA"
     gene            1814..3067
                     /gene="murA"
                     /locus_tag="ACZPB6_05590"
     CDS             1814..3067
                     /gene="murA"
                     /locus_tag="ACZPB6_05590"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1183980.1"
                     /translation="MQQLRINGGNRLVGEVRASGAKNAALPILAAALLTADDLVLHNV
                     PDLADVRTMLKLLEGMGVKVTRSGTDVTLNAGNVTSTKAPYELVKTMRASVLTLCPLA
                     ARFGSAEVSLPGGCAIGARPVDQHIRGLRQLGATVDIDHGYVRATAQRLKGAHIVTDM
                     VTVTGTENLVMAAVLAEGETVIENAAREPEVVDLCDCLNAMGAHITGAGTPVISIEGV
                     ERLHGAEHTVIADRIEAGSFLVAGVVTQGDVLVTHCQPSQMEVVLDKLRATGAVLDIG
                     ADWVRVRMTGRPKPVSIRTVPHPGFPTDMQAQFMTLNCFAEGTANITETIFENRFMHV
                     PELCRMGAHITIEGHTAIVEGVAQLTGTTIMATDLRASASLVIAALAAQGSSTVDRIY
                     HLDRGYERMEEKLRALGADIVRIDV"
     gene            3083..3487
                     /gene="hisI"
                     /locus_tag="ACZPB6_05595"
     CDS             3083..3487
                     /gene="hisI"
                     /locus_tag="ACZPB6_05595"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIR1183981.1"
                     /translation="MTHKEFEMNWIDEVNFNEEGLVPVIVQDVKTSRVLMLGWANREA
                     LESTVATGRGVYFSRSRGQVWCKGESSGNHQVVVGMQLATDGQSVIYRVDPQGGVASH
                     TGHASCFWRELVPGGWRECEPVVRAPEDMYEK"
     gene            3724..4065
                     /locus_tag="ACZPB6_05600"
     CDS             3724..4065
                     /locus_tag="ACZPB6_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR1183982.1"
                     /translation="MQLDDCLFCRIARGEIPAAKVYEDDEVLAFKDIHPKAPVHFLII
                     PKKHIQSLAHAEPEDNALLGKMLGLTRKLAMQEGAVNGFRVIINTGRDGGQEVDHLHI
                     HVLGGPQPWTK"
     gene            4076..4303
                     /gene="tatA"
                     /locus_tag="ACZPB6_05605"
     CDS             4076..4303
                     /gene="tatA"
                     /locus_tag="ACZPB6_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HIR1183983.1"
                     /translation="MGSLSVWHWLIVLVIVVLVFGTGKLKNMGKDLGGAIKGFKEGMK
                     EGDDDKSDAKKEIAQDKNTVDVEAKDKTNAS"
     gene            4434..5045
                     /gene="tatB"
                     /locus_tag="ACZPB6_05610"
     CDS             4434..5045
                     /gene="tatB"
                     /locus_tag="ACZPB6_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR1183984.1"
                     /translation="MFDFGFSEMLVIGVIALVVLGPERLPVVARTAGEWVGKAQRFVA
                     QVKSDIDRETELSELKRIQDEAKALADDVKSTVEKSAAQIESDVKSAAGEAKAVAEKT
                     QDNLKEIEETASGQTTVMSNDEISDFYGWGDNSDGPAAEPMYKWEEPKTFTKRYHAGP
                     SVDELAQEIAKLRRELGLREAQMGGSNRRLASRARTNRPRIYR"
     gene            5055..5852
                     /gene="tatC"
                     /locus_tag="ACZPB6_05615"
     CDS             5055..5852
                     /gene="tatC"
                     /locus_tag="ACZPB6_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR1183985.1"
                     /translation="MAENNKLLEAPDDPANEQPLVSHLIELRNRTVKASIAVLVIFAC
                     LSPFMKQIFDFLSQPLMVALPQGVKLLATGVVAPFMVPLKVTLFVAFLIALPYVLYQL
                     WAYVAPALYRREKRLALPILVSSIVMFAVGMAYCYFIVFRMVFQFIAGFSPDSVNFAP
                     DIDSYFSFVLTMFVAFGLTFEVPIVVMVLNHVGLASYEKLVKARPFVIVGAFVIAAIF
                     TPPDVLSQLLLALPLVILFQAGIWLVKFFGKSQDEIDGMPKDDEDEE"
     gene            complement(5924..7054)
                     /locus_tag="ACZPB6_05620"
     CDS             complement(5924..7054)
                     /locus_tag="ACZPB6_05620"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1183986.1"
                     /translation="MIRKLWLIFAQAVTVSAGIAVGLITVTDWRPFAPAQEAVHIPDF
                     SSAVERAAPAVVTIFARHATPGKRAEQIGTNWNSLPEEDFNTLGSGVLVSSDGFVLTN
                     YHVVASIQGLYVGLPDSGQAEAVLVGSDPETDLALIKISGESDLPFIGLGDTSKLKVG
                     QSVLAIGNPFDVGQTVTSGIISALGRHGLGLNSYEDFIQTDAAINQGNSGGALVNTEG
                     ELIGINTAIFSPEQSDGFIGIGFAIPSSIISRVLPSLMEGKTVERGYLGFVPRQLSRE
                     FAMDLGLTVTRGVMVSHVLGKSPAGRAGLRAFDIVKSLNGEEILGVNRLQQLIARLKP
                     GEPAELTVVRGAQTLTLTVTPTARPKNAREREKAGPAAINTI"
     gene            7109..>7399
                     /locus_tag="ACZPB6_05625"
     CDS             7109..>7399
                     /locus_tag="ACZPB6_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR1183987.1"
                     /translation="MQTQALIEHLNAYLNAASFKDYAPNGLQVEGKPEIHRIVLGVSA
                     SQALIDHACSMGADCILVHHGWFWKGERSEVVGMKQRRLKALLKNDINLIAYH"
BASE COUNT         1457 a         2245 c         2311 g         1386 t
ORIGIN      
        1 gcacgcgttc aacgtgatgg tcgacgatgc ggcgctcaag cccgtcacga agggctatcg
       61 ctgggtgacg ccgcagttcg tccgggacgg cgtctccaac gcgatcgaca acctcggcga
      121 agtgaacaac acgctcaaca acgtgctgca gggcaaggtg gatcagggca tcacgtccgc
      181 gttccgcttc atgctcaatt ccacgatcgg cgtgttcggt ctcttcgacg tggcgacctg
      241 ggtcggcatg gaaagggctc ccgaagactt cggccagacg ctcggcgtct ggggcgtgcc
      301 tgagggcccg tatttcgagc tgccgcttct cggtccctcg ggcggacgcg acatcttcaa
      361 gtggcccgtg cagaccgcca ccaatcccat cacctatctg acgtgggacg agtgggagtg
      421 gagcacggcg tatgcggtcg tcgacgcggt cgacacccgc tcccgcatga tggacgcagg
      481 tcttgacgac atgcgcgcca acgtggtcga cgagtacgtt gcgatccgcg acgcctaccg
      541 tcagtcgcgc cgcatggccg ttgccgacgg tcagatcgac gaggatgccc agctcaacag
      601 tctgacgccc ctgagttttg acgacgagga ggccgaggac gacgccggca agccggctga
      661 aacggacaag gacaaggaaa agtaatcatg cagagacgta ctttggttct cggcggcgcc
      721 tgcggcctcg cgcttgcggc catgcccgcc cttgcggccg aggccgtcga tcccttcgag
      781 tggatcgtcg ccctcaacaa cagcattctt gacgagatcc gcgcggatcc caagctgcac
      841 tcgggtgatc agacggcgct tcaggcgctc gtcgaccgtc gcgtcatggc ggccgcggac
      901 tttgcgatga tgacgcgcat gacgatcggc ccgaagtggc gaaaggccac gaaggaagaa
      961 cgccaacgcc tgatggacgg cttcgagaag ctgctcatcc gcgtctactc cggcgccctc
     1021 aagaccgtga cggaccacaa gtgcgaactc cgtccgaccc gcaacaggac gatcaaggac
     1081 gagatggtca tccgcaccat cctcaagtcg acgggcaatc ccgacatcgg catcgacttc
     1141 cgcatctacc gcgacaaggc gggcgagtgg aagatcgtcg acgtcaacgt cgagggcatc
     1201 tggatggtcg agaactaccg ctcgcagttc gcctccaccc tcaacaccga aggaattccc
     1261 ggtctgatca agctcctcga ggagaaggcc gacggtctgg tcgatgccaa tgcccacaag
     1321 gtgcagtaag ccatgaagtt cggaaccgag agcattacct tccgcgaggc caagaaggcc
     1381 aaggacgaca ttgccatcgc tgtgcgaaac ggcgacttcg tgctcgactt cgagggcgtg
     1441 catcgcatcg acagcacggt tctcgcgctc tcgctacaca tgttgcgcga ggcgaagaag
     1501 gcggggagca cgctccgcat cctgtcggcg ccgaggggct ttgcggatct ggtgaagctc
     1561 tacgggcttg agacgcttct catgccggcc cttgaagagg cgtgacggcg gtgcaggcgg
     1621 actgaatgga cggcgcgggt ttgaacccgc gcctttttct tgggcgcgga gtccctcccg
     1681 tcgcctccga tacaattgaa gccgcaagcg gattctccgc cgacgcctgc ccgattgtgg
     1741 tagcgttcaa agatcgggtc cgcgcagcgg atgaagacag aacacctcta cagacaaaag
     1801 aaatcccaac atcatgcaac agctcagaat caacggaggc aaccgcctcg tgggcgaagt
     1861 tcgcgcgtcc ggcgccaaga acgccgcact tcccatcctt gcggcggcgc tgctcacggc
     1921 ggatgacctg gtgcttcaca acgtgccaga cctggccgac gtgcgcacga tgctcaagct
     1981 cctcgagggc atgggcgtca aggtgacgcg atcgggcacg gacgtgacgc tcaatgcagg
     2041 caacgtgacg agcacgaagg ctccgtacga gctcgtgaag acgatgcgcg cctccgtgct
     2101 gacgctctgc ccgcttgccg cgcgcttcgg aagcgccgag gtgagcctgc ctggcggctg
     2161 cgccatcggc gcccgccccg tggaccagca catccgcggc ctgcgccagc ttggcgcgac
     2221 tgtggacatc gaccacggat atgttcgtgc gacggcgcag cgcctcaagg gcgcgcacat
     2281 cgtgaccgac atggtcacgg tgaccggcac ggagaatctg gtgatggccg ccgtgctcgc
     2341 cgagggcgag acggtgatcg aaaatgccgc ccgcgagccc gaggtggtcg atctgtgcga
     2401 ctgcctcaac gccatgggag cgcacatcac cggcgcgggc acgcctgtga ttagcatcga
     2461 gggcgtcgag cgcctgcacg gcgccgagca cacggtcatc gccgaccgca ttgaagccgg
     2521 atcctttctc gtcgcaggcg tggtaacgca gggcgacgtg ctggtgaccc actgccagcc
     2581 ctcccaaatg gaggtggtgc tcgacaagct ccgcgccacg ggcgccgtgc tcgacatcgg
     2641 cgccgactgg gtgcgcgtgc gcatgacggg ccgtccgaag cccgtttcca tccgcacggt
     2701 gccgcatccc ggctttccca ccgacatgca ggcgcagttc atgacgctca actgctttgc
     2761 cgaagggacc gccaacatca ctgagactat cttcgagaac cgcttcatgc acgtgccgga
     2821 gctctgccgc atgggcgccc acatcacgat cgaaggccat acggccatcg ttgagggcgt
     2881 tgcgcagctc acgggtacga cgatcatggc aaccgatctg cgcgcctccg cctcgctcgt
     2941 cattgcggcg ctcgcggctc agggctcgag tacagtggac aggatctacc atctggaccg
     3001 cggctatgaa cgcatggagg agaagctccg cgcgctcggc gccgacattg tccgcattga
     3061 tgtctgaact tgaaataaag acctgactca taaggagttt gagatgaact ggattgatga
     3121 agttaacttc aacgaagagg gcctcgtgcc cgtcatcgtt caggacgtga agaccagccg
     3181 cgttctgatg ctcggctggg ccaacaggga ggcgctcgag tcgaccgtcg ccaccggtcg
     3241 cggcgtctac ttctcgcgct ccagaggaca ggtctggtgc aagggcgagt cgtccggcaa
     3301 ccatcaggtc gtggtgggca tgcagctcgc cacggatggc cagtccgtca tctaccgcgt
     3361 ggacccgcag ggcggcgtcg cgtcccatac gggacatgcg agctgcttct ggcgcgaact
     3421 cgtgccgggc ggctggcgcg agtgcgagcc tgtcgtgcgc gcgcccgagg acatgtacga
     3481 aaaatgacat cctcccgggt ggaaatactt gaatttgtaa agatgcgggg aaatcccccg
     3541 tttgcgtcga aacctatacc gaagtaggtg gcgtggatgg ggaattccct ccataattca
     3601 agcgctttgt ctggcaatgg taagctacgt gtaacccagg ccagtaaaat gctatgacag
     3661 ggtgcgaaat gagcgcgatc ccgtcttttg cccgggaacg gctctcttct tccagaggtt
     3721 tacatgcagc ttgacgattg cttgttctgc cgcatcgccc gtggcgaaat tccggcagcc
     3781 aaagtttatg aagatgatga agtgctggcc ttcaaggaca tccatccgaa ggctcccgtg
     3841 cacttcctga tcattcccaa gaagcacatt cagtctctgg ctcacgccga gccggaggac
     3901 aacgctcttt tgggtaaaat gcttgggttg actcgaaagt tggccatgca ggaaggtgcc
     3961 gtcaacggtt tccgcgtcat catcaacact ggccgtgacg gcggtcagga agtcgatcac
     4021 ttacacattc atgttctggg cggtcctcag ccttggacga aataaggagt aaaaaatggg
     4081 ttccctttct gtttggcact ggctgatcgt tctcgtcatc gtcgtgctgg ttttcggtac
     4141 cggtaagctc aagaacatgg gcaaggacct tggcggcgcc atcaagggct tcaaggaagg
     4201 catgaaggaa ggcgacgacg acaagtccga cgcgaagaag gaaatcgccc aggacaagaa
     4261 cacggtcgac gtcgaagcca aggataagac caacgcttct tgatccgcac ggccctgacg
     4321 ggccatgtgg cttgccgcac tgacggcgtg tctgtcagtg ctggcgctta agctaacggc
     4381 cgcggcagtc tcaggggctg accgcggctc tttagtgaaa gacaggaata attatgtttg
     4441 actttggttt cagtgagatg ctggtgatcg gtgtgatcgc cctcgtggtc ctcggaccag
     4501 agcgtctccc cgtggtcgca cgcacagccg gcgaatgggt cggaaaggcc cagcgctttg
     4561 tcgcccaggt gaagtcggac atcgaccgcg aaacggagct ctccgaactt aaacgcattc
     4621 aggacgaggc caaggctctt gccgacgacg tcaagtcgac ggtcgagaag tcggccgccc
     4681 agattgaaag cgacgtcaag tctgccgccg gcgaagccaa ggccgtcgcc gaaaagaccc
     4741 aggacaacct caaggaaatt gaggagactg cgtccggcca gactaccgtg atgtccaacg
     4801 acgagatctc ggacttctac ggctggggcg acaattcgga cggtccggcc gccgaaccca
     4861 tgtacaagtg ggaggagccc aagaccttca cgaagcgcta tcatgcgggt ccgtcggttg
     4921 acgagcttgc gcaggaaatc gccaagcttc gccgcgaact cggtcttcgc gaagcccaga
     4981 tgggcggcag caaccgtcgt ctggcttccc gtgcgcgcac gaaccgtccg cgcatctacc
     5041 gataaagaac aatcatggct gaaaacaaca aactacttga ggcgccggac gatcccgcga
     5101 acgagcagcc gctcgtcagc cacctgatcg aattgcgcaa ccgcacggtc aaggcgtcga
     5161 ttgctgttct ggtcatcttc gcctgcctct cgcccttcat gaagcagatc ttcgacttcc
     5221 tgagccagcc gctcatggtc gccctgcctc agggcgtgaa gcttctggcc acgggcgtgg
     5281 tggcgccgtt catggtgccg ctcaaggtga cgctcttcgt ggcgttcctc atcgcgctgc
     5341 cgtacgtgct ctatcagctg tgggcgtatg tggcgccggc gctctacagg cgcgagaagc
     5401 gcctcgcgct tccgattctg gtctcctcga tcgtgatgtt cgccgtgggc atggcctact
     5461 gctacttcat cgtgttccgc atggtgttcc agttcatcgc aggcttctcg cccgacagcg
     5521 tcaacttcgc gcctgacatc gactcctact tcagctttgt gctgacgatg ttcgtcgcct
     5581 tcggtctcac gtttgaagtg ccgatcgtcg tgatggtgct caatcacgtc gggctcgcca
     5641 gctacgaaaa gctcgtgaag gcccgtccct tcgtgatcgt cggcgccttc gtgatcgccg
     5701 ccatcttcac gccgcccgac gtgctctcgc agctgcttct cgctcttccg ctcgtgatcc
     5761 tcttccaggc cggcatctgg ctcgtgaagt tcttcggaaa gtcgcaggac gagatcgacg
     5821 gcatgcccaa ggatgatgag gacgaggagt aagcttccga ctcatgcatg aaaaaaagcg
     5881 cattcccaga gggatgcgct tttttctttg ggccttggat tcgtcagatc gtgttgatcg
     5941 cggcggggcc tgccttctcg cgttcgcgcg cgttcttcgg acgggcggtt ggcgtcacgg
     6001 tcagcgtgag ggtctgcgcg cctcgcacca ccgtcagctc ggcgggttcg cccggcttga
     6061 ggcgggcgat gagctgctgg aggcggttga cgccgaggat ctcctcgccg ttgaggctct
     6121 ttacgatgtc gaatgcacga aggcccgccc tgcctgcggg gctcttgccg agcacatgac
     6181 tcaccatcac gcctcgggtg acagtgagac cgaggtccat ggcgaattca cgcgagagct
     6241 ggcggggcac gaagccaaga tagccgcgtt caacggtttt gccctccatg aggctcggaa
     6301 gcacgcggct gatgatcgag ctcggaatcg caaagccgat gccgatgaag ccgtccgact
     6361 gctcgggcga aaagatggcc gtgttgatgc cgatgagctc gccctcggtg ttgacgagcg
     6421 cgccgcccga attgccctgg ttgatggcgg cgtccgtctg gatgaagtcc tcatagctgt
     6481 tgaggccgag cccgtggcgc ccaagggccg aaatgatgcc cgaggtgacc gtctggccga
     6541 cgtcgaaggg gttcccgatg gcaagcacgg actggccgac cttgagcttc gaggtgtcgc
     6601 cgagaccgat gaagggcagg tcgctctcgc cggaaatctt gatgagggca aggtcggttt
     6661 cggggtcgct gcctaccagc acggcttcag cctgtccgct gtcgggcaag ccgacataga
     6721 gtccctggat cgaagcgacg acgtggtagt tcgtgagcac gaagccgtcg gacgagacga
     6781 ggacgccgct gccgagcgtg ttgaagtcct cttcaggaag gctgttccag ttggtgccga
     6841 tctgctcggc acgctttccc ggggtggcat gccgtgcaaa aatagtgacg acggcgggag
     6901 ccgcacgttc gacggcgctg ctgaagtcgg gaatgtgcac ggcctcctgt gcaggcgcga
     6961 acggacgcca gtccgtgacg gtgatgagac cgaccgcaat gcccgccgac acggtgacgg
     7021 cttgtgcaaa aatgagccac agcttgcgga tcatcgaacg gaacctccga gcggaaggct
     7081 caggaacagc aaatgggagt agatcattat gcagactcaa gccctgattg aacatttgaa
     7141 cgcttatctt aacgcggcgt cattcaaaga ctacgctccg aacggccttc aggtcgaggg
     7201 aaagcccgaa attcatcgca tcgtgcttgg cgtcagcgca agccaggcgc tcatcgacca
     7261 tgcctgcagc atgggggcgg actgcattct cgtgcaccac ggttggttct ggaagggcga
     7321 gcgctccgaa gtggtcggca tgaagcagcg ccgcctcaag gcgctcctga agaacgacat
     7381 caatctcatc gcctatcac
//
LOCUS       DBMDTI010000090         7326 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2691_length_7326_cov_3.085260, whole genome shotgun sequence.
ACCESSION   DBMDTI010000090 DBMDTI010000000
VERSION     DBMDTI010000090.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2691_length_7326_cov_3.085260"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="ACZPB6_05630"
     CDS             complement(<1..287)
                     /locus_tag="ACZPB6_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1183988.1"
                     /translation="MKLPVYLDYSATTPCDPRVVDKMVPYLYEKFGNPASHSHSYGWE
                     AEKAVEEARGHVAALIGADPREIVWTSGATESDNLAIKGAAHFYKDKGRHL"
     gene            complement(379..837)
                     /locus_tag="ACZPB6_05635"
     CDS             complement(379..837)
                     /locus_tag="ACZPB6_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIR1183989.1"
                     /translation="MKLTTKGRFAVTAMVDLALHEEEAPISLTQISERQQISVAYLEQ
                     IFCKLRRAGLVASVRGPGGGYRLGAPAGDITAAAIIEAMDEDIDATQCRGDGSCLGGA
                     ACLTHHLWEDLNRTTRKFLEGVTLAGMVEAHAARHGKQVVSAVIPVRKHA"
     gene            complement(1058..1570)
                     /locus_tag="ACZPB6_05640"
     CDS             complement(1058..1570)
                     /locus_tag="ACZPB6_05640"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR1183990.1"
                     /translation="MIKVLFVCTGNICRSPTAEAIFRKKVAEAKLDDVIFSDSAATSG
                     HHVGETPDVRAQLACRKRGLDIKEHRAREIAPEDFAKFDLILVMDWENLTDLQHRSPP
                     QFKHKIHLLMRYANDYDAAVVPDPYYGLNEGFNQVLDYCTDACGGLLETLERKARMMQ
                     KSLQATKAAG"
     gene            complement(1778..3814)
                     /gene="uvrB"
                     /locus_tag="ACZPB6_05645"
     CDS             complement(1778..3814)
                     /gene="uvrB"
                     /locus_tag="ACZPB6_05645"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR1183991.1"
                     /translation="MAQIVRYPDSPFILHQPFPPAGDQPSAIEALSEGLEDGVMFQTL
                     LGVTGSGKTYTMANVIARLGVPALIMAPNKTLAAQTYAEMRDFFPENAVEYFVSYYDF
                     YQPEAYVPARDLFIEKDAAVNEHIEQMRLAATKSILERRDTIIVATVSAIYGIGKPES
                     YTEMRLILREGDRKDQRRLITQLVKMQYRRTDTDFVRGTFRVRGDTIDVFPAEHAESA
                     VRIELFDDEVEAVHLFDPLTGRIEQKVPRFVVYPASHYVTPREEVIRAMTGIKAELKE
                     RLAELYADGRIVEAQRLQQRTMFDLEMLDQVGFCKGIENYSRHLTGLAPGEAPPCLID
                     YLPSDSIMFFDESHVMMGQLGGMYRGDRARKETLVNYGFRLPSALDNRPLRFDEFERK
                     MRRSVFVSATPAAYELEKSSGEVVEQVVRPTGLVDPRVEVRPAVTQVDDLLSEITLTV
                     KKEERVLVTTLTKRMAEDLTDFLSEHGVRVRYLHSDIDTVERVEIIRDLRAGRFDVLV
                     GINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGRAILYADRMTD
                     SMERALAETGRRREKQLAYNAEHGIVPRGVKKEIRDIIDGVYRGPDVAKPASRRVEET
                     DFGALDEKSLSARLKELEARMMDFARNLDFEKAAQVREEIKHLREQAFGASAEA"
     gene            4015..5211
                     /locus_tag="ACZPB6_05650"
     CDS             4015..5211
                     /locus_tag="ACZPB6_05650"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HIR1183992.1"
                     /translation="MTREYFASLEPRPDDPILGLVELYKNDPRDTKVNLGVGIYLDNE
                     GRLPLMKAVGAAEQRLAQRGAPHGYIPMSGLPAYCAAIQKLVFGATNEAVLSGRIATI
                     QSLGGTGALRLGAVFAHEYLGLTKAVVSNPTWGNHITLFEHAGLKVDRYPYLNKEKNG
                     VDFEAMKACLEGLEAGTVMLLHACCHNPTGYDLNAEQWAEVTRIIQERDLLPFLDIAY
                     QGFGSGLDDDAAAVRALCAAGVSFMVSSSCSKNFGLYGERAGGLHVVTPNADEAAVVT
                     SIIKAIVRAEYSNPATHGAAIVNEVLNDEELYAVWEDEVTGMRTRIRSMRESLVAAGE
                     AVGADLSFAVRQAGMFSFTGLNAEQMLALREKYGVYGVANGRICIAGLNTENVTYVAK
                     AIAGVR"
     gene            5311..5386
                     /locus_tag="ACZPB6_05655"
     tRNA            5311..5386
                     /locus_tag="ACZPB6_05655"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5344..5346,aa:Asn,seq:gtt)
     gene            5429..5504
                     /locus_tag="ACZPB6_05660"
     tRNA            5429..5504
                     /locus_tag="ACZPB6_05660"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5462..5464,aa:Asn,seq:gtt)
     gene            6013..7077
                     /locus_tag="ACZPB6_05665"
     CDS             6013..7077
                     /locus_tag="ACZPB6_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HIR1183993.1"
                     /translation="MRYPAAKLESLARDFLEATGSSEDEARVVAEHMIGANLRGHDSH
                     GVGMIAMYAQYLNEGKLIPNAPLNVIKDTGPVLQLDGCRGYGQRIGREATDMAIERAK
                     KHGICMYTIGRSCHLGRIGTYGEQAAAAGMVSIHFVNVNHFFPLVAPFNGSKARFGTN
                     PMCVAMPGTDAHGPFILDFATSIVAMGKTRVAYLAGKKFDEPVVLDVDGHPTNDPTPM
                     WQEGEKGALMAFAKHKGSGLAMACELLAGLLAHGETIQPGHERDGSIVNNMTAFMIDP
                     SVLGNLDWMKAEFDAMVDYVKSSPAPDPVNNPVLTPGEPERLRMADRLANGVEISDGE
                     MAAILNVCQAAGVHHHWEVI"
     gene            complement(7238..>7326)
                     /gene="rrf"
                     /locus_tag="ACZPB6_05670"
     rRNA            complement(7238..>7326)
                     /gene="rrf"
                     /locus_tag="ACZPB6_05670"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1354 a         2329 c         2239 g         1404 t
ORIGIN      
        1 atgaggtgac ggcccttgtc cttgtagaag tgcgcggcac ccttgatggc gagattgtcg
       61 ctctccgttg cgccggacgt ccagacgatc tcgcggggat cggcgccaat gagggcagca
      121 acgtgaccgc gggcctcttc aacggccttc tcggcctccc agccgtagct gtggctgtgg
      181 ctggcgggat tgccgaactt ctcgtagaga tacggaacca tcttgtcgac cacgcggggg
      241 tcgcagggcg tcgtcgcgga gtagtcaagg taaacgggca gcttcatcat gaatcctttt
      301 tgggaacgga tcgtcggtgc cggcgatgct tgtaggttta gagggggctc ttttgtgctt
      361 cggctgaaag gcggcgcctc aggcgtgctt tcgcaccgga atgacggcgg acacgacctg
      421 cttgccgtgg cgggctgcgt gagcctcgac catgccggcg agcgtcacgc cctccagaaa
      481 ctttctcgtg gtccggttga ggtcctccca cagatggtgc gtgaggcagg cggcgccgcc
      541 aaggcagctg ccgtcgccgc ggcactgcgt ggcgtcgatg tcctcgtcca tcgcctcgat
      601 gatcgcggcg gcggtaatgt cgcccgccgg agcgccgaga cggtacccgc cgcccggacc
      661 gcgaacgctc gcaacgagcc ctgcacggcg gagcttgcag aagatctgct cgagataggc
      721 cacggagatc tgctggcgtt cggaaatctg cgtgagggag atgggggcct cctcttcatg
      781 cagggccagg tccaccatgg ccgtgacggc aaatcgtcct ttggtcgtca gcttcaagat
      841 gcagctccaa tttgacgggg gttgaatgac gggaaatcac gggggagtga ccgaatcccg
      901 accaagttgg tcggatatta gcgaagaata cccgactaat caagtcaatt attgagtcta
      961 cgcaaaaagg aggacaaaac ttacacgcca gaaagccccc catcaacgcg aaaagcccgc
     1021 gggagcgggc ttttcaatgc acgggccgtc ctgaggatca gccggcggcc ttcgtggcct
     1081 gaaggctctt ctgcatcatg cgggccttgc gctcgagcgt ctcgagcagg ccgccgcagg
     1141 cgtcggtgca gtagtcgagc acctgattga agccctcgtt gaggccgtag tacggatccg
     1201 gcacgacggc ggcgtcatag tcgttggcgt agcgcatcag cagatggatc ttgtgcttga
     1261 actgcggagg agagcggtgc tgcagatcgg tgagattctc ccagtccatc acgaggatga
     1321 ggtcgaactt cgcgaaatcc tcgggcgcaa tctcacgcgc acggtgctcc ttgatgtcga
     1381 gaccgcgctt gcggcaggca agctgagccc tcacgtcggg cgtttcgccg acatgatgtc
     1441 cgctcgtggc cgcgctgtcc gaaaaaatca cgtcatcaag cttggcctcc gcgaccttct
     1501 tgcggaagat cgcctcggcg gtcggactgc ggcagatgtt gcccgtgcac acaaaaagaa
     1561 ccttaatcac tttctagttt tccgaaattc tgcagtgccc cgtccgtcct cgggaggggg
     1621 caggttttcc aaaccaacgt gcatatgcta ctgcagaagg cgtgttctgc ctagtctcaa
     1681 tttcacccgt catcccgcct tcattgccgc agaggcatat gcaacctgaa caaattttgc
     1741 ggggaaagcc gctaaggcct cttcccgagc cgttcgctca ggcttcagcg gatgctccga
     1801 acgcctgctc cctcaggtgc ttgatctcct cgcgcacctg agcggccttc tcgaagtcga
     1861 ggtttcgcgc gaagtccatc atgcgggctt cgagctcctt gagccttgcg ctgaggctct
     1921 tctcgtcgag cgctccgaag tcggtctcct cgacgcgcct cgaggcgggc ttcgcgacgt
     1981 cggggcccct gtagacgccg tcgatgatgt cgcggatctc cttcttgacg ccgcgcggca
     2041 cgatgccgtg ctcggcgttg taggcgagct gtttctcgcg gcggcggccc gtctcggcaa
     2101 gcgcgcgctc catcgagtcg gtcatgcggt cggcatagag gatcgccctg ccgttgaggt
     2161 ttcgagcggc tcggccgatc gtctggatga gcgagcgctc gctgcgcagg aagccctcct
     2221 tgtccgcgtc gagaatggcg acgagcgaga cctcgggaat gtcgaggccc tcacgaagca
     2281 ggttgatgcc cacgagcacg tcgaacctgc ccgcgcgcag gtcgcggatg atctcgacgc
     2341 gctcgacggt atcgatgtca gagtggaggt acctcacgcg cacgccgtgc tcggaaagga
     2401 agtccgtgag gtcctcggcc atgcgctttg tgagcgtcgt cacgagcacg cgctcctcct
     2461 tcttcacggt gagcgtgatc tcggagagca ggtcatcgac ctgggtgacg gcgggacgca
     2521 cctcgaccct ggggtcgacg aggcccgtcg gacgcacgac ctgctcaacc acctcgcccg
     2581 aactcttctc gagctcgtag gctgcgggcg tcgccgagac gaagacgctg cggcgcatct
     2641 tgcgctcgaa ctcgtcgaag cggagcgggc ggttgtcgag cgcggacgga agacggaagc
     2701 cgtagttgac gagcgtctcc ttgcgcgcgc ggtcgccgcg gtacatgccg ccgagctgtc
     2761 ccatcatcac gtggctctcg tcgaagaaca tgatgctgtc ggacgggagg tagtcgatga
     2821 gacagggcgg cgcctcgccg ggcgcaaggc ccgtgaggtg gcgggagtag ttttcaatgc
     2881 ccttgcagaa gcccacctga tcgagcatct ccagatcgaa catcgtgcgc tgctgcaggc
     2941 gctgcgcctc tacgatgcgc ccgtcggcat agagctcggc gagacgctcc ttgagctcgg
     3001 ccttgatgcc cgtcatggca cgtatgacct cctcgcgggg cgtgacgtag tggcttgcgg
     3061 ggtacacgac gaagcggggc accttctgct cgatgcggcc ggtgagcgga tcgaagagat
     3121 gcacggcctc gacttcgtcg tcgaagagct cgatgcgtac ggcgctctcg gcatgttctg
     3181 cggggaagac gtcgatcgtg tcgccgcgaa cgcggaatgt gccgcgcacg aagtccgtgt
     3241 cggtgcggcg gtactgcatc ttgacgagct gcgtgatgag ccggcgctgg tccttcctgt
     3301 cgccctcgcg aaggatgagc ctcatctcgg tgtagctctc gggcttgccg atgccgtaga
     3361 tcgccgagac ggtggcgacg atgatcgtgt cgcggcgctc gagaatggac ttggtggcgg
     3421 caagacgcat ctgctcgatg tgctcgttga cggccgcgtc cttctcgatg aagaggtcgc
     3481 gtgcggggac gtaggcctcg ggctggtaga agtcgtagta ggagacgaag tactccacgg
     3541 cgttctcagg gaagaagtcg cgcatttcag cgtaggtctg cgcggcgagc gtcttgttgg
     3601 gcgccatgat gagggcgggg acgccgagcc tcgcgatgac gttcgccatc gtgtaggtct
     3661 tgcccgaacc cgtcacgccg agcagcgtct ggaacatgac gccgtcctca agcccctcgg
     3721 agagcgcctc gatggccgag ggctgatcgc ccgccggcgg aaagggctgg tgaaggatga
     3781 agggcgagtc gggatatcgg acgatctgtg ccactggaaa cctcggtcag ggatctgaga
     3841 tggttagaca gtcgattgta tgctgttttg aagcgaacac gcagtacctc atagtcctta
     3901 tggcatctga ccaatctcga aataccctca gagagccgcc cgccgagccg acgggcggtc
     3961 cctcggttgg ttatagtgaa tggagcttct ttttcaacac gggctccaca caaaatgact
     4021 cgcgaatact ttgcttccct tgaaccccgt cctgacgatc ccattctcgg cctcgttgaa
     4081 ctctacaaga acgatccccg cgacacgaag gtcaacctcg gcgtcggcat ctacctcgac
     4141 aacgaaggca ggcttccgct catgaaggcc gtcggcgcgg ccgaacagcg cctcgcccaa
     4201 cgcggcgctc cgcacggcta catcccgatg tcgggccttc ccgcctactg cgccgccatc
     4261 cagaagctcg tcttcggcgc cacgaacgaa gccgtcctct cgggccgcat cgccaccatc
     4321 cagtctctcg gcggcacggg cgcgctccgt ctaggcgcag tgttcgcgca cgaatacctc
     4381 ggcctcacga aggccgtcgt ctccaacccc acctggggca accacatcac gctttttgag
     4441 cacgcaggcc tcaaggtcga ccgctacccc tacctcaaca aggagaagaa cggcgtcgac
     4501 ttcgaagcca tgaaggcctg tctcgagggc ctcgaggccg gcacggtcat gcttctgcac
     4561 gcctgctgcc acaacccgac cggctacgac ctcaacgccg agcagtgggc cgaggtcacg
     4621 aggatcattc aggaacgcga cctgcttccc ttcctcgaca tcgcctatca gggcttcggc
     4681 tccggccttg acgacgacgc agccgccgtc cgcgccctgt gcgccgccgg cgtctccttc
     4741 atggtcagtt cctcctgctc gaagaacttc ggcctctacg gcgaacgtgc gggcggtctg
     4801 catgtcgtca ctcccaacgc agacgaagcc gcagtggtca cctccatcat caaggccatc
     4861 gtccgcgccg aatactccaa ccctgccacg cacggcgccg ctatcgtcaa cgaagtcctc
     4921 aacgacgaag agctctacgc cgtctgggag gatgaggtga ccggcatgcg cacccgcatc
     4981 cgctccatgc gcgagagcct ggtcgccgcg ggcgaggccg tcggcgccga cctctccttc
     5041 gccgtccgtc aggcgggcat gttctcgttc acaggcctca acgccgaaca gatgctcgca
     5101 ctgcgtgaga agtacggcgt ctacggcgtg gccaacggcc gcatttgcat cgccggcctc
     5161 aacactgaaa atgtaaccta cgtcgccaaa gcgattgcag gagtaagata agaagaaaaa
     5221 aatcggcacg aggcataccg tgtttatggt attttcaaga aattctcttg ccaaagactc
     5281 aaagtctccg atatacttcg tgtccttgat tccccgatag ctcagtcggt agagcgacgg
     5341 actgttaatc cgcaggtcgc tggttcgagc ccagctcggg gagccaagtc agattggcca
     5401 atccgactta ccggccaatt caaagtactc cccgatagct cagtcggtag agcgacggac
     5461 tgttaatccg caggtcgctg gttcgagccc agctcgggga gccacgaatt cgaaaagcgg
     5521 cgctttcgca agaaggcgcc gctttttttc gtccctgcaa tgcgccgcac gcctccgcct
     5581 tcgggcttgg atttcgcatt agtccggaac cactaggcac ttttccctgc ccactttgcc
     5641 gcgcgtcacg attcccgcgt ttccccttct cgtctaagca ctttacccat tgaggcaccg
     5701 ccttgtggca tgtatcgttg acttcggcga atcggattgg gttatccgga tcgtcccggt
     5761 ggagagaaac ctttctgtct gtcttccctc cccggtttgg ctcttccggt acaggtggac
     5821 cgaaagagcc gggtcttggg ccgcgtcgac gctcgcatgc gtccgcggcc cccttttttg
     5881 gacccgcttt cttgcgaagc gcctcgagga ctaggtcatg agcctgatgt ccgccaccct
     5941 cccgtcgtcc tagaatgtag gaatcggaat cctgcggatt tcgaaccgac tttcatataa
     6001 aggacaacag ccatgcgcta cccagcagca aaacttgaga gccttgcccg tgacttcctc
     6061 gaggccaccg gcagcagcga ggacgaagcc cgcgtcgtcg ccgaacacat gatcggcgcg
     6121 aaccttcgcg gccacgacag ccacggcgtg ggcatgatcg ccatgtacgc ccagtacctc
     6181 aacgaaggca agctcattcc caacgccccg ctcaacgtca tcaaggacac gggccccgtg
     6241 ctccagctcg acggttgccg aggctacggc cagcgcatcg gccgcgaggc gaccgacatg
     6301 gccatcgagc gcgcgaagaa gcacggcatc tgcatgtaca cgatcggtcg cagctgccat
     6361 ctcggccgca tcggcaccta cggcgagcag gctgccgccg caggcatggt ctcgatccac
     6421 ttcgtgaacg tcaaccactt ctttccgctc gtcgcgccgt tcaacggctc caaggcccgc
     6481 ttcggcacaa atcccatgtg cgtggcgatg cccggcaccg acgcgcacgg tccgttcatc
     6541 ctggacttcg ccacgtcgat cgtggcgatg ggcaagaccc gcgtcgccta tctcgccggc
     6601 aagaagttcg acgaacccgt ggtgctcgac gtcgacggtc atcccaccaa cgaccccaca
     6661 ccgatgtggc aggaaggcga gaagggtgcc ctgatggcgt tcgccaagca caagggctcg
     6721 ggcctcgcca tggcctgcga gctgcttgca ggcctcctcg cgcacggcga gaccatccag
     6781 ccgggccacg agcgcgacgg cagcatcgtc aacaacatga cggccttcat gatcgatccg
     6841 tccgtgctcg gcaacctcga ctggatgaag gccgaattcg atgcgatggt cgactacgtc
     6901 aagagctctc cggctcccga ccccgtcaac aaccccgtgc tcacgccggg cgaacccgaa
     6961 cgcctccgca tggccgacag gcttgccaac ggcgtcgaga tctccgacgg cgaaatggcg
     7021 gccattctga acgtctgcca ggccgcaggc gtgcaccacc actgggaagt catctgaggc
     7081 tccccaaggg cctctgaagg cgtctgagac acttcctgag gtcccggccc gcaggccccg
     7141 tctgcgggcc gttgcgcatc cgcggggccg aaatccttga ggcgttgaaa aacaaaaacc
     7201 ccggtctctt tcgaaaccgg ggtttttgga atggtgagcc tgacgatgtc ctactttcac
     7261 tggaaataca accaactatc atcggcgata aggtgtttca ctgtcctgtt cggaatggga
     7321 aggagt
//
LOCUS       DBMDTI010000091         7214 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2730_length_7214_cov_2.378462, whole genome shotgun sequence.
ACCESSION   DBMDTI010000091 DBMDTI010000000
VERSION     DBMDTI010000091.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2730_length_7214_cov_2.378462"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(85..381)
                     /locus_tag="ACZPB6_05675"
     CDS             complement(85..381)
                     /locus_tag="ACZPB6_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013933.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR1183994.1"
                     /translation="MSSLFALFLVTSLANILTPGLGVVMIITLAAEYGWRRTMSGALG
                     TALGIAFLFIAGMSGVGVIVASSPMLFAAIKLLGACFLIWLSLKLPCLKTEDSL"
     gene            complement(394..1563)
                     /locus_tag="ACZPB6_05680"
     CDS             complement(394..1563)
                     /locus_tag="ACZPB6_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1183995.1"
                     /translation="MQFVDFAPVKLVFGPGRLEALRTEPLPGTRALLLTSNGRSMERI
                     GALARVTAILEERGVAVVHMGVVEPNPTVSTVMKVAARMKDERCDFLVALGGGSVMDC
                     AKISGLMATNPGELWDYVQGGTGGRRAFEHDPLPLVAITTTAGTGSEVDAGGVITNPE
                     TREKVGIGHPGCFPKLAIVDPELMVSVPPALTAYQGFDALFHAVEGYVGKRPNFMSDM
                     YALEAVRHLAAWLPKAVADGTDIKAREHVAFANTMAGFVMTTTRLTSHHAMEHALSAF
                     HPELPHGAGLIMLSMAYFGHMVAVGAQSERFVALARAMGVEDAVQPSDFLSALSRLIH
                     DCGMQDLKMSDWGITCEELPAMTEVCRSAGAHAFEQDANYFTFEDTLAVYEAAFR"
     gene            complement(2487..4838)
                     /gene="feoB"
                     /locus_tag="ACZPB6_05685"
     CDS             complement(2487..4838)
                     /gene="feoB"
                     /locus_tag="ACZPB6_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HIR1183996.1"
                     /translation="MTKKAICVVGNPNCGKTTLFNALTGAKQDVGNWPGVTVEKKTGS
                     YAWRGEEVEITDLPGIYSITPSSAAGEDERVARDYILTGETECVVNIIDASNLERNLY
                     LTAQLIEMRVPMLVVVNMLDVAKQHKIEIRLNELEKALGCPVVGIVASRSSGISELKD
                     RISEFLANPVVPPMKIRFDERITTCIDKLEAELKAGGVARPRWFALQVMESAPGIEEK
                     LPDGMFERAKSIMEPVSADFDGDLDIAIAGARYEFVGAIAADAVVRKGKPGETLTDRI
                     DRIVLNRWLGLPIFLFVMYLMFLFTQNLGAAFIDFFDILVGGVLVDGFGALLETLGAP
                     DWLRVMLANGIGGGIQTVATFIPVVFFLFFFLAILEDSGYMARAAFVMDRLMRALGLP
                     GKAFVPLLVGFGCNVPAIMATRTMDRASDRIITIMMAPFMSCGARLPVYVLFATAFFP
                     TNGQNLVFGLYLIGILAAVVTGLLLKRIALPGAASAFVMEIPPYHIPTVKGVMLRTWD
                     RLKGFVLRAGRVIVVIVACLSILNSMGTDGTWGHEDTNESVLSEIGRTIVPVLEPMGV
                     SEENWPAAVGIFTGVLAKEAVVGTMNSLYDSMARAKNAENGVAEEASEDEAGWSFGAT
                     LVEALESVRTNLADLGGALLDPAGIHVDDLSDTAAAAEEQEVAVDTIDMMQQLFGGGF
                     AAFCYLLMVLLYMPCGAAVATVWREAGTAWTLFLCGWTTALGYTSATIVYRLGTFAEN
                     PTYSIVAIALSVAILAGMLLWMRTFAKKNGGKGRKVIPIYATR"
     gene            complement(4883..5110)
                     /locus_tag="ACZPB6_05690"
     CDS             complement(4883..5110)
                     /locus_tag="ACZPB6_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1183997.1"
                     /translation="MLLKELSPGARGRIVALRKEQPEYRRRLLMLGATPGAEFEVVRI
                     APLGDPVEIRVRGSMISVRRDEAAIMEVSVD"
     gene            complement(5132..5377)
                     /locus_tag="ACZPB6_05695"
     CDS             complement(5132..5377)
                     /locus_tag="ACZPB6_05695"
                     /inference="COORDINATES: protein motif:HMM:NF015956.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1183998.1"
                     /translation="MMNNTPAKTLRRLTDLETGSCASVARIKLDAAEEARLSELGLRP
                     GAEIRRLHGEAGQSILIGVGDGRIGVNFDTAKRIYVF"
     gene            5742..6599
                     /locus_tag="ACZPB6_05700"
     CDS             5742..6599
                     /locus_tag="ACZPB6_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HIR1183999.1"
                     /translation="MALRDIRLWLHDGTPAGARTVFEGDAFVSILGADDAAREALALL
                     GGREAVTVFAGPDAMPGRLVAAVVSGDVREAAAGCLPENGRTPFTSVVVLGAPGAAHL
                     EYAVRAAVASSGGVTFLDGVEAQEEAGPLPAAWTSLAAVAGGAPAWLAERHDVRDGDC
                     ARAFPGLPLSCTGRGAQARARLVRGGILILAGSRCSPSDATPSLIAPIRDAIKRLVDA
                     GDLEKRDGVWTFVRDVSLASPVQAASIVMRTSAGAAAWETEDGDTLAERLAPRHHTSA
                     HTAEGRDKP"
     gene            6933..>7214
                     /locus_tag="ACZPB6_05705"
     CDS             6933..>7214
                     /locus_tag="ACZPB6_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184000.1"
                     /translation="MNETTKYGLFFLGGVVLGALGAVAVTRGKLDVKPLATDLLSAGI
                     DLKEKALSAVESAKEDIADAVAEAQVKSQEKKARAEEAEAAKVEDKTAEA"
BASE COUNT         1333 a         2092 c         2535 g         1254 t
ORIGIN      
        1 gaacgggaaa gcggtctgcc gcgcacactt tgtacagacg gtgacgttca ggcggctttg
       61 ccccccgaaa gagctgtctg acggtcaaag tgaatcctct gtcttcagac aggggagctt
      121 caacgacagc cagatgagga agcaggcgcc gaggagcttg atcgccgcga agagcatggg
      181 actcgaggcg acgatgacac ccacgccgga catgcccgcg atgaagagga acgcgatgcc
      241 gagcgccgtg ccgagggcgc ccgacatggt ccggcgccac ccgtactcgg cggcgagcgt
      301 gatgatcatc actacgccga gccccggcgt caggatgttc gcgaggctcg tgacgagaaa
      361 gagcgcgaag agactcgaca tgaggccctt ttcttagcgg aaggcggctt cgtacacggc
      421 gagcgtgtct tcgaaggtga agtagttcgc gtcctgctcg aaggcgtggg cgcctgcaga
      481 gcggcagact tcggtcatgg cgggcagctc ctcgcacgtg atgccccagt cgctcatctt
      541 caggtcctgc atgccgcagt cgtggatgag acgcgagagc gcgctcagga agtcggaggg
      601 ctgcacggcg tcctcgacgc ccatggccct ggcgagcgcg acgaagcgtt cggactgtgc
      661 gcccacggcg accatgtggc cgaagtaggc catcgagagc atgatgaggc ctgcgccgtg
      721 cgggagttcg ggatggaagg ccgagagcgc gtgctccatc gcatggtggc tcgtgaggcg
      781 cgtagtcgtc atgacgaagc ccgccatggt gttggcgaag gcgacgtgtt cgcgggcctt
      841 gatgtcggtg ccgtcagcga cggccttggg aagccacgcg gcgagatggc ggacggcctc
      901 aagcgcatac atgtcgctca tgaagttcgg acgcttgccc acgtagcctt cgacagcgtg
      961 gaagagcgcg tcgaagccct ggtaggcggt gagggcgggc ggaacggaga ccatgagttc
     1021 ggggtcgacg atagcgagct tcgggaagca gcccgggtgc ccgatgccga ctttctccct
     1081 cgtttcggga ttggtgatga cgccgccggc gtcgacttcg ctgccggtgc ccgcggtcgt
     1141 cgtgatcgcg acgagcggaa gcggatcatg ctcgaaggcc ctgcgtccgc cggtaccgcc
     1201 ctggacgtag tcccagagct cgccgggatt cgtagccatg aggcccgaga tcttcgcgca
     1261 gtccatgacg gagccgccgc ccagcgcgac gaggaagtcg catctctcgt ccttcatgcg
     1321 ggccgccacc ttcatgacgg tcgagaccgt cgggttgggc tcgacgacgc ccatgtgaac
     1381 cacggcaacg ccacgttcct cgagtatggc ggtgacgcgc gcgagcgcgc cgatgcgctc
     1441 catcgagcgg ccgttcgagg tgagaagcag ggccctggtg ccgggcaggg gctcggtgcg
     1501 aagggcctcg agcctgccgg gaccgaaaac aagcttgacc ggggcgaagt cgacgaattg
     1561 catgaaagtc tctccaaaca gttgaaggcc ggccggaaag ggagaaccgg acggcgtcgt
     1621 tctaagcagt attgcgtagg gacattctag cgccgaaggc gcgcggacca agaggggctt
     1681 gaagccgggg ggaagaaggc ccggagagag gcttgatgag aaaagcaggg gcgcaaaaca
     1741 aaaaaggccg gtccgcgagt cggaatcggc cttcagtcag cgtgcaataa aaaagggaca
     1801 cgttcttcaa catgtcccgc gccttgtggt gagtggtctc aaaccgtcgg ctccttgatc
     1861 caggcttgcg gcagcttgca acacctgcta cccaaatgga ctcccaacag cgacccgtga
     1921 tttcgaagtt ccggtctgat acgcaaggaa ccacatcaac cacaacaaag ttaactatat
     1981 caggggttcc acggatttgc aagtcgacgt tgcattttgt tgcatccgtc gtccgcattc
     2041 cgcgtgcatg cggctcgtct tcacgaatcc gcttgccgcc tgacgcgttc ggggcccgtc
     2101 ccggaaggac gcttcccgac tgccgtgatg agcttaggca tttcctgcct ccggccgcct
     2161 tgtctcgagc tcgaagatcc ttgaggtgct tcgaatggac tcccaacagc ggctttcgac
     2221 tttcgcgtcc ttcggtcgga agaaccgcgt tcatctcagc gacttgaatg gtacaggaac
     2281 ccggccgtcg cgtgcggcga tctaacaaac acttgcaaac caatggaaac gtcatctgaa
     2341 ggcgactttt gtgacgactt cgtgacgtgc gagggaggaa ggcgggatgc aggggaaagg
     2401 ggccggcggt cgtgcgaccg ccggcccctt cgggaaggcc tggggagccg tgctgccgtc
     2461 agggcggagc ctgacggcgg cactgatcat cgcgtggcgt agatcgggat caccttgcgg
     2521 cccttgccgc cgttcttctt cgcgaaggtg cgcatccaga ggagcatgcc tgccaggatg
     2581 gcgacggaga gggcgatggc gacgatcgag tacgtcgggt tttcggcgaa ggtgccgagg
     2641 cggtagacga tcgtggcgga ggtgtagccg agcgccgttg tccagccgca caggaagagc
     2701 gtccatgccg tgcccgcttc gcgccagacg gtcgcgacgg cggcgccgca gggcatgtag
     2761 agcaggacca tgagcaggta gcagaaggct gcgaagccgc ctccgaagag ctgctgcatc
     2821 atgtcgatcg tgtcgacggc gacttcctgt tcttcggcgg cagcggccgt gtcggagagg
     2881 tcgtcgacgt ggatgccagc cgggtcgagg agagcgccgc cgagatcggc gaggttcgtc
     2941 ctgacgcttt cgagggcctc gacgagcgtg gcgccgaagg accagccggc ttcgtcttca
     3001 gaggcttcct cggcaacgcc gttctcggcg ttctttgcgc gggccatgct gtcgtagagc
     3061 gagttcatcg tgccgaccac ggcttccttg gcgaggacgc ccgtgaagat gccgacggcg
     3121 gcgggccagt tctcttcgga gacgcccatg ggctcgagga ccggaacgat cgtgcggccg
     3181 atttcggaga ggacggactc gttcgtgtcc tcatggcccc aggtgccgtc ggtgcccatc
     3241 gagttgagga tggagagaca ggcgacgatc acgacgatga cgcggccggc gcgcagcacg
     3301 aagcccttga ggcggtccca ggtgcgcagc atcacgccct tgacggtcgg gatgtggtac
     3361 ggcgggatct ccatcacgaa ggccgaggcg gcgcccggaa gggcgatgcg cttgagcagg
     3421 aggcccgtga cgacggcggc gaggatgccg atgaggtaga ggccgaagac gaggttctgg
     3481 ccgttggtcg ggaagaacgc cgtggcgaag agcacgtaga ccgggagacg ggcgccgcag
     3541 ctcatgaacg gagccatcat gatcgtgatg atgcggtcgg aggcgcggtc catcgtgcgg
     3601 gtggccatga tggcgggcac gttgcagccg aagccgacga gcagcggcac gaaggccttg
     3661 cccggcaggc cgagggcgcg catcaggcgg tccatgacga atgcggcgcg ggccatgtag
     3721 ccggagtcct cgagaatggc gaggaagaag aagaggaaga agacgaccgg gatgaaggtt
     3781 gcgaccgtct ggatgccgcc gccgatgccg ttggcgagca tgacgcgcag ccagtcgggc
     3841 gcgccgaggg tttcgagcag ggcgccgaag ccgtcgacga ggacgccgcc cacgagaatg
     3901 tcgaagaagt cgatgaaggc cgccccgaga ttctgcgtga agaggaacat caggtacatc
     3961 acgaagagga agatcggcag gccgagccag cggttgagca cgatgcggtc gatcctgtcc
     4021 gtgagcgtct cgccgggctt gcccttgcgg acgacggcat cggcggcgat cgcgccgacg
     4081 aattcgtagc gggcgcccgc gatggcgatg tcaaggtcgc cgtcgaagtc ggcggagacg
     4141 ggctccatga tcgacttggc gcgctcgaac atgccgtcgg ggagcttttc ctcgatgccg
     4201 ggagcggatt ccatcacctg gagggcaaac cagcgcggac gggcgacgcc gcccgccttg
     4261 agctcggcct cgagcttgtc gatgcaggtg gtgatgcgct cgtcgaagcg gatcttcatc
     4321 ggcgggacga cggggttggc gaggaactcg ctgatgcggt ccttgagctc gctgatgccc
     4381 gaggagcggg aggcgacgat gcccacgacg gggcagccga gggccttctc aagctcgttg
     4441 aggcggattt cgatcttgtg ctgcttcgcg acgtcgagca tgttgacgac gacgagcatc
     4501 ggcacgcgca tctcgatgag ctgtgcggtg aggtagaggt tgcgctcgag gttggaggcg
     4561 tcgatgatgt tgacgacgca ttccgtctcg cccgtgagga tgtagtcgcg agcgacgcgc
     4621 tcatcctcgc ccgctgcgga ggacggcgtg atggagtaga tgccgggaag gtcggtgatc
     4681 tcgacttcct cgccgcgcca ggcgtaggag cccgtcttct tctcgacggt cacgccgggc
     4741 cagttgccca cgtcttgctt ggcgcccgtg agcgcattga agagcgtcgt cttgccgcag
     4801 ttcggattgc cgacaacgca gatggctttt ttcgtcatga tgttcgcttg aatggttgga
     4861 aagtgaatgc ggtcggcgtg aatcagtcca cggagacttc catgatggcg gcttcgtcgc
     4921 ggcgcacgct gatcatgctg ccgcgaacgc ggatttcaac cggatcgccc agaggtgcga
     4981 tgcggaccac ttcgaattcg gcgccgggcg tggcgccgag catcaggaga cgacggcggt
     5041 attcgggctg ttcctttctg agggcgacga tcctgccgcg ggcgccggga gagagttcct
     5101 tgagaagcat gagagtggat cctttttctg attagaagac gtagatgcgc ttggccgtgt
     5161 cgaagttgac gccgatgcgg ccgtcgccga cgccaatgag aatgctctgg ccggcttcac
     5221 catgaagacg gcggatttcg gcgccggggc gaagaccgag ctcggacaga cgcgcctctt
     5281 cggcggcatc gagcttgatg cgggcgaccg aggcgcagga gccggtctca agatccgtga
     5341 gacggcggag cgtttttgcg ggagtgttgt tcatcatttg cggcttgtcc gagtgtgagt
     5401 aaggacgatt ccgccgttgt ggacggaatg gtgatcggaa tataactgat aaccattcgc
     5461 attacaactt ttgtcaagac gggcgccgac ggcttcggta gcgttgcggc agtggggaaa
     5521 acattgaatg gagtgagaat cattctcctt atgcagcatg tatttttttt gacttaagtc
     5581 aaatgagaag caatctcatt ccctattgcg agagcgaagg ccataggaga ggacttggag
     5641 tcccgttcgg ggcgccttga ggcggaactc gcggaggcgt ctgcagaggc actagaatga
     5701 ggcaggagat tttcaggaca cggcacaagg gaggcgcctc catggcactg cgcgacatca
     5761 gactctggct tcacgacggc acgcccgcgg gcgcgaggac cgtcttcgag ggggacgcct
     5821 tcgtctcaat cctcggagcg gatgacgccg ctcgggaggc gcttgcgctt cttggcgggc
     5881 gtgaggccgt gacggtgttc gcggggcccg atgcgatgcc gggacgcctg gtggcggccg
     5941 tcgtctcggg agacgttcgc gaggctgcgg cggggtgcct gcctgaaaac ggcaggacgc
     6001 ccttcacgtc ggtcgtcgtg ctcggcgctc cgggggcggc gcaccttgag tatgcggtcc
     6061 gcgcggcggt ggcgtcctcg ggcggcgtga ccttccttga cggcgtcgag gcgcaggaag
     6121 aggcggggcc gctgcccgcc gcctggacct cgcttgcggc cgtggcgggc ggagcgcccg
     6181 catggcttgc cgaacgtcac gacgtccgtg acggagactg cgcccgcgcg ttccccggtc
     6241 tgccgctctc ctgcacgggg cgcggcgcac aggcccgcgc acggcttgtg cgcggcggca
     6301 tcctcatcct tgcagggagc cgctgctcgc cctccgacgc aacgccgtcc ctcatcgccc
     6361 cgatccgcga cgccatcaag cgcctcgtcg acgcgggcga tcttgaaaag cgcgacggcg
     6421 tctggacctt cgtgcgagac gtgagcctcg cctcgcccgt gcaggccgcc tcgatcgtaa
     6481 tgcgcacctc ggcgggagcc gccgcctggg agaccgagga cggcgacacg ctcgccgaaa
     6541 gactcgcacc gcgccaccac acgagcgcac acacagccga gggtagggat aaaccctgat
     6601 aaaaattggt gcgaaaacaa cagccatcct gagcacggag ccctttttcg acgggtttcg
     6661 tgcttttttg cattcgcttg agcaggacgc accaaagacg gcgataatgg aatttcgcgg
     6721 aaacgattgc atttcaggcg ttttcgttgt ctttgcgcga caggtcgggc gtgccgcccg
     6781 atttttggcg tggcaatgag aattgttcgc gtatagaatg cagtcggcgg ggcggcttca
     6841 ggagacgcat tcccgcgcac attttcagga cggccggacc agcacgccgg cggccgctcc
     6901 tttttcccac gaaggcgtgc ggagaacgaa aaatgaatga aacgacgaag tacggcctct
     6961 ttttcctcgg cggcgttgtc ctcggtgctc ttggtgcagt ggcggtgacc cgcggcaagc
     7021 tcgacgtcaa gccgctggcc acggacctcc tctccgccgg catcgacctc aaggaaaagg
     7081 ccctgtccgc agttgaaagc gcgaaggagg acatcgccga cgccgtcgcc gaagcacagg
     7141 tcaagtctca ggagaagaag gcccgcgccg aagaggccga agccgccaag gtcgaggaca
     7201 agacggctga agcc
//
LOCUS       DBMDTI010000092         7141 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2766_length_7141_cov_3.720312, whole genome shotgun sequence.
ACCESSION   DBMDTI010000092 DBMDTI010000000
VERSION     DBMDTI010000092.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2766_length_7141_cov_3.720312"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="ACZPB6_05710"
     CDS             complement(<1..335)
                     /locus_tag="ACZPB6_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIR1184001.1"
                     /translation="MNANEVLMPGETLGLMGGGQLGRMFAQAAATMGYHVAVLEPGKA
                     CPAGEVSREQIETKYDDVAGLGALAGRVKAVTTEFENVPAPSLEYLEKAGLRVCPPSA
                     AVAVTQDRNV"
     gene            complement(353..850)
                     /gene="purE"
                     /locus_tag="ACZPB6_05715"
     CDS             complement(353..850)
                     /gene="purE"
                     /locus_tag="ACZPB6_05715"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR1184002.1"
                     /translation="MTDAVKPIVGIVMGSNSDWEVMKNAAEMLKAFDIPFEARVVSAH
                     RMPDEMFDYAETARDRGIRVIIAGAGGAAHLPGMIAAKCTIPVCGVPVPSKYLRGQDS
                     LYSIVQMPKGVPVATFAIGEAGAANAALYAAQILSVTDAGLAQKLADFRAAQNAKARA
                     MELPL"
     gene            complement(1010..1903)
                     /locus_tag="ACZPB6_05720"
     CDS             complement(1010..1903)
                     /locus_tag="ACZPB6_05720"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR1184003.1"
                     /translation="MAGLSQINIPSLKKLYSGKVRDTFAVGDDKLLIVASDRISAFDV
                     ILGDPIPGKGKVLTSLTDFWFKQLEGVCPNHLTGIDPESVVKPEEVPMVKGRSCVAMR
                     LKPILIECVARGYLIGGGWKDYCATGCVCGIKLPEGLQMGQKLPEPIFTPAAKAEVGT
                     HDENISFERVVELYGEEIAEKIRSYTLELYRRASEYAATKGIIIADTKFEFGIDEEGT
                     IRLMDEVCTPDSSRFWPADQYNVGVSPPSYDKQFIRDWLEAQPWDKTPPAPKVPDEVI
                     EKTSDKYREALRRLAETDIEQ"
     gene            complement(2032..2451)
                     /locus_tag="ACZPB6_05725"
     CDS             complement(2032..2451)
                     /locus_tag="ACZPB6_05725"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR1184004.1"
                     /translation="MTTPNSQDKNATDLKGKKGLRRLINATGYSMKGLSAAYRNEAAF
                     REEVLLACVLIPCALLLGLPAVETVLLIGSVLLLMLVEILNSAIEAVVDRIGPELHPL
                     SGRAKDLGSAAVFIAMIILGVTWLLIAAPALWAFLAG"
     gene            complement(2753..3817)
                     /gene="fba"
                     /locus_tag="ACZPB6_05730"
     CDS             complement(2753..3817)
                     /gene="fba"
                     /locus_tag="ACZPB6_05730"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432158.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR1184005.1"
                     /translation="MALVSMRQLLDHAAENGYGVPAFNVNNLEQVQAIMAAASEVGAP
                     VIMQASAGARKYAGEHFLRHLISAAVEAYPDIPVCMHQDHGQSPSVCQGAIRMGFTSV
                     MMDGSLMADGKTISTYDYNVEVTRRVVEMSHCIGVSVEGELGCLGSLETMKGDKEDGH
                     GTDAVMTREQLLTDPDQAADFVKATQLDALAIAIGTSHGAYKFTRKPTGETLSIQRVK
                     EIHERLPNTHLVMHGSSSVPQEYLATIRQFGGDMRETYGVPVEEIQEAIKHGVRKVNI
                     DTDIRLAMTAATRQYLFENPSKFDPRDFLKVARKAATELCKQRYLQFGCEGMASRIKP
                     LPLDVVAARYADGTLRQNVL"
     gene            complement(3992..5323)
                     /locus_tag="ACZPB6_05735"
     CDS             complement(3992..5323)
                     /locus_tag="ACZPB6_05735"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1184006.1"
                     /translation="MIDRRRLVLGSASTLLLSLAPWQIARGARMVDVRMWPAEEYTRV
                     TLEYDSPLNFKYFFVRSSKPLRLVVDIKGLELTVALQKQIAAVKPDDPYIAAMRIGQF
                     QPDTVRLVMDLKTDVKPEVFQLQPFANYKYRLVFDIYPAHPTDEISRLLNGLEEGSEA
                     PDGDPLGSLLAGLDKGAAESPVKPPLEEPEPKEPVRPSRPSKPKKPSKSIKPKLIIVV
                     DPGHGGEDPGAIGRGRNREKTVVLQIAKRLAALINDDPGMKAIMTRNTDHFVSLGGRV
                     AIARRAKAHLLVSIHADAWIKASARGSSVFCLSERGATSAAARWLAKNQNESDLIGGV
                     NIASVDRQVASVLVDMTSSWTISYSLGLGASVLNEMKSVNRLHKKHVEQAGFAVLKGQ
                     GIPSILVETAFISNPTEEKLLINGRHQQKIAQAIYDGIRKQVAANPSLLTG"
     gene            complement(5320..5820)
                     /gene="tsaE"
                     /locus_tag="ACZPB6_05740"
     CDS             complement(5320..5820)
                     /gene="tsaE"
                     /locus_tag="ACZPB6_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR1184007.1"
                     /translation="MASLTFEASLPHPDDTDKLGARLADAVSKEAGAVAGHGLAVRLE
                     GNLGAGKTSLVRAMLRRLGFAGPVKSPTFSLVETYPVAGLTLNHFDFYRFEEPEEFED
                     AGFRDLFGPGFVSATEWTEKASPYVPDADLVIELALEGLGRRAVLKGLTPLGEALVAH
                     VSEAGE"
     gene            6035..6110
                     /locus_tag="ACZPB6_05745"
     tRNA            6035..6110
                     /locus_tag="ACZPB6_05745"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6068..6070,aa:Thr,seq:cgt)
     gene            6283..6912
                     /locus_tag="ACZPB6_05750"
     CDS             6283..6912
                     /locus_tag="ACZPB6_05750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1184008.1"
                     /translation="MQKTGIIVLGSVAAMLPALAQASETVRLAEPDLAMEGSLMTALK
                     NRHSTREYAAKDLTGADLSNLLWATAGVNCPDGRRTAPTALNLQEIGVYVVTAKGAFS
                     YRHEGHELVKLTDKNLMPLVAMQQDFVNTAPLALVYVGDLTKLPGERGAQMAAVDAGI
                     AVQNALLYCSAAGLACVPRATMNVDGLAQALSLPKGAVPIMNVVVGMPK"
BASE COUNT         1274 a         1994 c         2489 g         1384 t
ORIGIN      
        1 acgttgcggt cctgggtgac ggcgacggcg gcggacggcg ggcagacgcg caggcccgcc
       61 ttctcgaggt attcgagcga gggggcgggg acgttctcga attcggtcgt gacggccttc
      121 accctgcctg cgagcgcgcc gaggccggcc acgtcgtcgt acttcgtttc gatctgctcg
      181 cgggagactt cgcccgcggg gcaggccttg ccgggttcga gcacggcgac gtggtagccc
      241 atcgtcgcgg cggcctgggc gaacatgcgg ccgagctggc cgccgcccat caggccgagc
      301 gtttcgcccg gcatcagcac ttcattggcg ttcatatgac ggaatttcct cgttagagcg
      361 ggagttccat ggcgcgggcc ttggcgttct gggcggcgcg gaagtcggcg agcttctggg
      421 cgaggccggc gtccgtgacg gagaggatct gggcggcgta gagcgcggcg ttggcggcgc
      481 ccgcttcgcc gatggcgaag gtggcgacgg gcacgccctt gggcatctgg acgatggagt
      541 agaggctgtc ctggccgcgg aggtacttgg acggaacggg cacgccgcag acggggatcg
      601 tgcacttggc ggcgatcatg ccgggcaggt gggcggcgcc gcctgcgccc gcgatgatga
      661 cgcggatgcc gcggtcacgg gcggtttcgg cgtagtcgaa catttcgtcg ggcatgcggt
      721 gagcgctcac gacgcgggct tcgaacggaa tgtcgaatgc cttgagcatt tcggcggcgt
      781 tcttcatcac ctcccagtcg gagttgctgc ccatcacgat gccgacgatc ggcttaacgg
      841 cgtcagtcat tggaaaacct cgctgtaaaa atcaaaaaac aaaaacataa aagaacgaca
      901 ggaaagccgt gctttcctct cgttcctcag aatctatgag tatagacaag aaaggaccgg
      961 caggcatgaa gactgtcggt cctttcaggg gcatcacagg ggatgcggat tactgttcga
     1021 tgtccgtttc ggcaaggcgg cgcagggctt cgcggtactt gtcggaagtc ttttcaatga
     1081 cttcgtccgg aaccttcgga gcgggcgggg tcttgtccca gggctgggct tcgagccagt
     1141 cgcggatgaa ctgcttgtca tagctcgggg gcgagacgcc gacgttgtac tggtcggccg
     1201 gccagaagcg gctggagtcg ggcgtgcaca cttcgtccat caggcggatg gtgccttcct
     1261 cgtcaatgcc gaactcgaac ttcgtgtcgg cgataatgat gcccttcgtg gcggcgtatt
     1321 cggaggcgcg gcgatagagc tcgagcgtgt agctgcggat cttctcggcg atttcctcgc
     1381 cgtagagctc gacgacgcgt tcgaacgaga tgttttcgtc atgcgtgccg acttcggcct
     1441 tggcggccgg cgtgaagatc ggttcgggga gcttctggcc catctggaga ccttcgggaa
     1501 gcttgatgcc gcagacgcag ccggtggcgc agtagtcctt ccagccgccg ccgatcaggt
     1561 agcctcgggc gacgcactcg atcaggatgg gcttgaggcg catggcgacg caggagcggc
     1621 ccttgaccat aggcacttcc tcaggcttga cgacggattc gggatcaatg ccggtgaggt
     1681 ggttcgggca gacgccttcg agctgcttga accagaagtc ggtgagagag gtgagcacct
     1741 tgcccttgcc cggaatcggg tcgcccagaa tgacgtcgaa tgcggaaatg cggtcgctcg
     1801 cgacgatgag cagcttgtca tcgccgacgg cgaacgtatc gcgcaccttg ccggagtaga
     1861 gcttcttcaa ggagggaatg ttgatctggg aaagaccagc catgttgaaa tctccctttg
     1921 agagatggtt aactgaaatg tggtgccgtc attgtaatgc cgatcatccg ttcgctacgc
     1981 tttttgacta gtagatttgg ctaggtcggc atgaggatcg gcaatgtttg gtcagcctgc
     2041 caaaaacgcc cagagcgcag gcgctgcgat cagaagccac gtgacgccga ggatgatcat
     2101 cgcgatgaag accgctgcgc tgccgaggtc cttggcgcgt ccgctcaagg ggtgaagctc
     2161 gggaccgatg cggtccacga ccgcctcaat ggcggagttg aggatctcga cgagcatcag
     2221 gagaaggacg gagccgatga ggagcaccgt ctcgacggcg ggtaggccga gcaggagcgc
     2281 gcagggaatg agcacgcagg cgaggagtac ctcctcgcgg aaggcggcct cgttcctgta
     2341 ggcggccgag aggcccttca tggagtagcc cgtggcgttg atgagtcggc gcaggccctt
     2401 cttgcccttg aggtccgtgg cgttcttgtc ttgggagttg ggtgtggtca tgatgcagtt
     2461 gacgttctct ccgccgtgaa cgacagagat tctctactga gtcagcagct tgcgctgctg
     2521 atcacttcgg tggattcctg ctgctgattc ggcgccagct ttttagatcg ttccgaattc
     2581 acttgagcct gagagttttt ctcagactcc ctacaggcta acgagcttcg tccgcgctct
     2641 tatatctccg ccctgaacgg agctttacgc cgcaattggg taaaagcgcg cccgccctga
     2701 cgcgtggtcg ggcgggcgac gtgtggttca gccgatcaat tcggactctc ggttagagca
     2761 cgttctggcg aagcgtgccg tcggcgtagc gggcggccac gacgtcgagc gggagcggct
     2821 tgatgcggga ggccatgcct tcgcaaccga actggaggta gcgctgcttg cagagctccg
     2881 tggcggcctt gcgggcgacc ttcaggaagt cgcgcgggtc gaacttggag gggttctcga
     2941 agaggtactg gcgggtggcg gccgtcatgg cgagacggat gtccgtgtcg atgttgacct
     3001 tgcgcacgcc gtgtttgatg gcttcctgga tttcctcgac gggcacgccg tacgtttcgc
     3061 gcatgtcgcc gccgaactgg cggatcgtgg cgaggtattc ctgcggaacg gaggaggagc
     3121 cgtgcattac gagatgggtg ttcggcaggc gttcgtggat ttccttgacg cgctggatgg
     3181 agagcgtttc gccggtgggc ttgcgcgtga acttgtaggc gccgtgggac gtgccgatgg
     3241 cgatggcgag ggcgtcgagc tgcgtggcct tgacgaagtc ggcagcctga tccgggtccg
     3301 tgaggagctg ttcgcgcgtc atgacggcgt cggtgccatg gccgtcttcc ttgtcgccct
     3361 tcatcgtttc gagggagccg aggcagccga gttcgccttc gacggagacg ccgatgcagt
     3421 gggacatttc aaccacgcga cgggtgactt cgacgttgta gtcgtaggtg gagatcgtct
     3481 tgccgtcggc catgagggag ccgtccatca tgacggaggt gaagcccatg cggatggcgc
     3541 cctggcagac ggacgggctc tggccgtggt cctgatgcat gcacacgggg atgtccggat
     3601 aggcctcgac ggcggcagag atcagatggc gaaggaagtg ctcgccggca tacttgcggg
     3661 cgccggcgga ggcctgcatg atcacggggg cgccgacttc gctcgcggcg gccatgatgg
     3721 cctgaacctg ttcgaggttg ttgacattga aagcaggaac gccgtagccg ttttcggcgg
     3781 cgtgatcgag cagctgacgc atggagacga gtgccatgac gatgaactcc gaatctgatg
     3841 aaagggaaaa acaaaagggc ggcgcaggcc ttgcgggccg ttttcgggcc gcccgaaact
     3901 gatcagatgc attgtatagg gacaatgcgt ctagggaaag aggtttatcc ctgcctgagg
     3961 ggcgaaggga cacaaaaaac gcgtcctccc gtcagcccgt cagcagggac ggattcgcgg
     4021 ccacctgctt gcggatgcca tcgtagatgg cctgggcgat cttctgctgg tgcctgccgt
     4081 tgatgaggag cttttcctcg gtcgggttcg agatgaaggc cgtctcgacg aggatcgacg
     4141 gaatgccctg acccttgagg accgcgaagc ccgcctgttc gacgtgcttc ttgtgcaggc
     4201 ggttgacgct cttcatctca ttgaggacgg aggcgccaag gccgaggctg tagctgatgg
     4261 tccagctcga ggtcatgtcg acgagcacgc tcgctacctg gcggtcgacg ctggcgatgt
     4321 tgacgccgcc gatcaggtcg gactcgttct ggttcttggc gagccagcgg gcggcggcgg
     4381 acgttgcgcc gcgttcggaa aggcagaaga cgctcgaacc tcgggcgctg gccttgatcc
     4441 aggcgtcggc gtgaatgctc acgagcaggt gcgccttggc gcggcgggcg atggccacgc
     4501 ggccgccgag gctcacgaag tggtccgtgt tgcgggtcat gatggccttc atgccgggat
     4561 cgtcgttgat gagcgcggcg aggcgcttgg cgatctggag cacgaccgtc ttctctcggt
     4621 tgcgtccgcg gccgatggcg ccagggtcct cgccgccgtg gccggggtcg accacgatga
     4681 tgagcttggg cttgatggac ttcgagggct tcttcggctt ggatggtctg gaggggcgga
     4741 cgggctcctt cggctcaggc tcctcgaggg gaggcttgac gggggattcg gccgcgccct
     4801 tgtcgaggcc tgcgagcagt gagccgagcg ggtcgccgtc gggtgcctcg ctaccttcct
     4861 cgaggccgtt gagcagacga ctgatctcgt cggtcggatg cgcgggatag atgtcgaaga
     4921 cgagacggta cttgtagttc gcaaagggct ggagctggaa gacctcgggc ttgacgtcgg
     4981 tcttgaggtc catcacgaga cggaccgtgt cgggctggaa ctgcccgatg cgcatggcgg
     5041 cgatgtaggg atcgtcgggc ttgacggcgg caatctgctt ctgcagcgcg acggtgagct
     5101 cgagcccctt gatgtcgacg acgaggcgca gcggcttcga cgagcggacg aagaagtact
     5161 tgaagttgag cggggagtcg tactcgagcg tgactcgggt gtactcctcg gcgggccaca
     5221 tgcgaacgtc gaccatgcgg gcgcctctgg cgatctgcca cggcgcaagg ctgagaagga
     5281 gcgtgctcgc agagccgagc acgaggcggc gtcggtcgat cattcgcccg cctccgaaac
     5341 gtgggcgacg agcgcttcgc cgagcggggt gagtcccttc aggacggcgc ggcggccgag
     5401 accctcgagg gcgagctcga tgacgaggtc cgcgtcgggc acgtagggcg acgccttttc
     5461 tgtccattcg gtcgcggaca cgaagcccgg accgaagagg tcgcgaaagc ccgcatcctc
     5521 gaactcctcg ggctcctcaa agcggtagaa gtcgaagtga ttgagcgtga ggcccgcgac
     5581 gggataggtc tcgacgagcg agaaggtggg gctcttcacg gggccggcaa agccgaggcg
     5641 gcgcagcatg gcgcgaacga gcgacgtctt gcctgcaccg aggttgccct caaggcgcac
     5701 ggccagaccg tgcccggcaa ccgcgcccgc ttccttcgag acggcgtcgg caagacgggc
     5761 gccgagcttg tcggtgtcgt cgggatgggg aagagacgct tcaaaagtaa gggatgccat
     5821 gtcggtcctt ctgagccgtg gagccaaaac tttgatttta gcccatgacg ggagcctgct
     5881 cggacgaggg aatcgttgcg gcatgcaagc caaaaacagc ctttcgcggg ataatgtaac
     5941 gaaatgtaat tcgctcttgc caaagcggat gaagtccctc ataattcaaa tcctcagtcg
     6001 aacgcgactt gctccgaacg actgacagat ggtggccggt gtagctcagc tggtagagca
     6061 gcgcattcgt aatgcgaagg tcgggagttc gaatctcttc tccggcacca cgagaattca
     6121 gaaaggatct gtacagcatt gtacaggtcc ttttttcatt tccgccggcc ttttttcaag
     6181 ttccggcagg cggcctgacc gcttcgaatc ttgcgctcgg acggggcgcg gtgctagtct
     6241 cattcggtga aaacatctct cttgcaaagg aggaaccgaa ttatgcaaaa gacgggcatc
     6301 atcgtgctgg gctcggttgc ggccatgctt cccgcactgg cccaggcgtc cgaaacggtc
     6361 aggctcgctg aacccgacct cgccatggag ggctcgctga tgacggcgct caagaaccgt
     6421 cactcgactc gcgagtacgc ggcgaaggac ctgacgggcg cggatctctc caatcttctc
     6481 tgggcgacgg cgggcgtcaa ttgtcccgac ggacgccgaa cggcgcccac ggcgctcaac
     6541 ctccaggaaa tcggcgtcta cgtcgtgacc gccaaggggg ccttttccta cagacacgaa
     6601 ggacacgagc tcgtgaagct cacggacaaa aacctgatgc cgctcgtcgc gatgcagcag
     6661 gacttcgtca acacggcgcc gctggcactc gtctatgtgg gcgatctgac caagctgccc
     6721 ggagagcgcg gcgcccagat ggccgcagtt gacgcgggca ttgccgttca gaatgcactt
     6781 ctctactgct ctgcggcggg gcttgcctgc gtgccgcgcg ccacgatgaa cgtggacgga
     6841 cttgcacagg cgctctcgct cccgaaggga gccgtgccga tcatgaacgt cgtggtcggc
     6901 atgccgaagt aggccgggcg ccaccttcct caatgcaggg agggctcggg aaaggtacgg
     6961 aaaaaggccc gcacatcatg tcgatctgcg ggccttttcc ttgtgtgctc ggcatgggca
     7021 cgttctaacg gatgaaagtt ccgagagggg ccggcagtgg caacctcata gccaaaagca
     7081 aggtgtgcgt cgcgaggcgt aggctgaaag aagctggagg caaacccttg agctgatgaa
     7141 c
//
LOCUS       DBMDTI010000093         7087 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2784_length_7087_cov_2.375608, whole genome shotgun sequence.
ACCESSION   DBMDTI010000093 DBMDTI010000000
VERSION     DBMDTI010000093.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2784_length_7087_cov_2.375608"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="ACZPB6_05755"
     CDS             <1..477
                     /locus_tag="ACZPB6_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184009.1"
                     /translation="DDLPLAQGWYVAKRQVQGKQVAPDRSVRMTMEFLERVAHPEAVL
                     KRLKKHMPGYGKNKAGVFEQLFPFLGTALWRTLGGVDPEYGHGSRLPELLERLCSLAG
                     TTIVDVACRQALELASRCLAKAGGRSSGTCVRQKARPAAPKNPRASGVTRMPQCCA"
     gene            complement(577..1923)
                     /gene="brnQ"
                     /locus_tag="ACZPB6_05760"
     CDS             complement(577..1923)
                     /gene="brnQ"
                     /locus_tag="ACZPB6_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HIR1184010.1"
                     /translation="MKSFTLKERCLIGLTLFSMFFGAGNLIFPPFLGALAGDATLPAM
                     SGFAVTAVLFPILGVAAVARSGGLTALGNRVHPAFAFLFTLLIYLSIGPCLAIPRTAG
                     TSFEMAIAPLFAGEGALSGTILGFEAHAVAQAAYSVFFFLLAFTIALNPEKLTQRLGK
                     ILSPTLLTLIAVLFIAAIVHPLGEYGAVRAPYDAAPFVRGFIEGYQTMDTLAALNFGL
                     IIAMNIRALGVTKDSGVVRETIFAGFVAGFLLITVYAALAHIGAEAGGAGLTGENGAQ
                     TLTGVVIQQFGHAGLFILGAIFFIACLNTCVGLLSCCSNYFRDTFPVLGYRGWLTVFA
                     VTSTIIANAGLTAILKFSVPVLVAIYPLALVLIILAFLHPFIEKHRFAYPMTMLFTGA
                     AAITAGFGQAGFKLPVLADFFAAMPFAAEGLDWMLPAAVGLVVGIALSLVTPKKAA"
     gene            complement(2191..2266)
                     /locus_tag="ACZPB6_05765"
     tRNA            complement(2191..2266)
                     /locus_tag="ACZPB6_05765"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2231..2233),aa:His,seq:gtg)
     gene            2538..3560
                     /locus_tag="ACZPB6_05770"
     CDS             2538..3560
                     /locus_tag="ACZPB6_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1184011.1"
                     /translation="MARGPHLSELAAEWQALGAKPQHVRRIFLAWAGLAPWEARKGES
                     FPKAVAEALPDIRERLDRLSHALPVRSAEAETVKLILGLEDGECVESVLLPRQGLCVS
                     TQVGCAVGCVFCMTGKGGLVRQLSSAEIVAQYCEARRVRSDVRKIVLMGMGEPSHNLD
                     AVCEAVEFFGNVCGLAHKDIVVSTVGDHRLFDRLPALSVRPALALSLHTTDNALRRKL
                     LTNSKDIPVETLLERTLDYADETGYPAQIEWTLMAGINDAPEQVERLADLIEGRYAMV
                     NFIAVNPVEGSGFRRPESAHMQDLITILRRRGTVATLRDSAAQDIEGGCGQLRARVIG
                     IRKEQA"
     gene            complement(4003..4302)
                     /locus_tag="ACZPB6_05775"
     CDS             complement(4003..4302)
                     /locus_tag="ACZPB6_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184012.1"
                     /translation="MTTVDTIAQAIKEKKALEFAYHGFDRVVVPYACGLTPTNEIKMI
                     GFQIGGGSNSGVTEKLRFYTAADIAGVAFSDAAYREATEEDKAVFAQLSKIYEQI"
     gene            complement(4566..4697)
                     /locus_tag="ACZPB6_05780"
     CDS             complement(4566..4697)
                     /locus_tag="ACZPB6_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184013.1"
                     /translation="MRHALFAQAHCMGGVHGTVRLGSNTLTICIINGMIAARKVASR"
     gene            5052..5852
                     /locus_tag="ACZPB6_05785"
     CDS             5052..5852
                     /locus_tag="ACZPB6_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1184014.1"
                     /translation="MNLSRLFAPIALVAAIALSGISGSALADTTLRIGVTPVPHADIV
                     NFIKPTLEKEGVKLEVVEFNDYIQPNLALDAGELDANYFQTIPYLEEMVKSRGLNLHI
                     LVSVHLEPMALYSKKVKSLADIPAGAKVAVPSDPTNEGRALKVLEHAGLIKLKDGATL
                     LSGIGDIVSNPKKLKFVELEPALLPRALDDVTAAVINANYALEAGFNPAKNAIALEEK
                     SSPFGNVVAIREGDDKKEAFQKLKKAITSPEVKKFIEEKYQGGVLPVF"
     gene            5940..>7087
                     /locus_tag="ACZPB6_05790"
     CDS             5940..>7087
                     /locus_tag="ACZPB6_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR1184015.1"
                     /translation="MTRTDKLVAAHWGVARVRMTDGRPALVPIVEDMRPSERMADYLA
                     GRDGAERLLAPKVREGYLRACREGRDPALTREGRGREAFVEVEWEEALELAARAIERT
                     YADHGPSAVWGRSYGWKNTGEVNNPIGLLRRLLMLKGGFVQTFNSYSTAAIAAIQKVI
                     AGSGDPDVPPAAEVLEHAERVILWGADPVVTNDIAWTTTLHDTAKVFEAMRTKEGLEV
                     VAVNPVKPETLDVTGGRWIAPLPGTDAAFALGLVHTLLATGRADRHFLETRTTGFETL
                     EACLNGRLDGVVRDAAWAAGICGLDEKTIRDFAEELAAHRTMIMLGWGPQRARYGEST
                     VWAVWALAAALGQIGGRGTGIGTRYHYSSGGAGASAGRGLPGLPVNVAP"
BASE COUNT         1374 a         2070 c         2348 g         1295 t
ORIGIN      
        1 gatgatcttc ctctggcgca gggttggtac gttgcgaagc ggcaggtgca ggggaagcag
       61 gtggctccgg acagaagcgt ccggatgacg atggagtttc tggaacgtgt tgctcatccc
      121 gaggccgtgc tcaagcgcct gaagaagcac atgcccggtt acggcaagaa caaggcgggc
      181 gtgttcgagc agcttttccc gttcttggga accgctctgt ggcgcacgct tggaggcgtg
      241 gatcccgagt acggtcatgg ttcgcgactg cctgaactcc tcgagcggct ctgctctctt
      301 gcgggcacca cgatcgtcga tgttgcgtgc cgccaggcgt tagaacttgc ttcgaggtgc
      361 ctggcgaagg ccggcggtcg ttcttcgggc acctgcgttc ggcagaaggc caggccggct
      421 gcaccgaaga atccccgcgc gtcaggcgtc acgaggatgc cgcagtgttg cgcctgaggg
      481 tgttccgtga agtgaacggt caaatgaaag gcgccttcgg aatccatgga attcgcgaag
      541 gcgccctgat tttgagtgac ttcagagggg aggccgtcag gcggccttct tcggcgtcac
      601 gaggctcagt gcgatgccga cgacgaggcc gacggcggcg ggaagcatcc agtcaaggcc
      661 ttcggccgcg aacggcatgg cggcgaagaa gtcggcgagg accggcagct tgaagcctgc
      721 ctggccgaag cccgccgtga tggcggcggc gcccgtgaag agcatcgtca tcgggtaggc
      781 gaagcggtgc ttttcgatga acggatgcag gaaggcgagg atgatgagca cgagcgcaag
      841 cggatagatc gcgacgagga cggggaccga gaacttcagg atcgcggtga ggccggcgtt
      901 ggcgatgatc gtgctcgtca cggcgaagac cgtgagccag ccgcggtagc cgagaaccgg
      961 gaacgtgtcg cggaagtagt tcgagcagca ggagagcagg cccacgcagg tgttgaggca
     1021 ggcgatgaag aagatcgcgc cgaggatgaa gaggccggcg tggccgaact gctggatcac
     1081 gacgcccgtg agggtctggg cgccgttttc gcccgtaagg cccgcaccgc ccgcttcggc
     1141 gccgatgtgc gcaagcgcgg catagaccgt gatgagcaga aagcccgcga cgaagcccgc
     1201 gaagatcgtc tcgcggacaa cgcccgagtc cttcgtgacg ccgagcgcgc ggatgttcat
     1261 cgcgatgatg aggccgaagt tgagggccgc gagcgtgtcc atcgtctgat agccctcgat
     1321 gaagccgcgg acgaacggcg ccgcgtcata gggggcgcgc acggcgccgt actcgccgag
     1381 cggatgcacg atggcggcga tgaagagcac cgcgatgagc gtgagcagcg tgggcgagag
     1441 gatcttgccg aggcgctgcg tgagcttttc ggggttgagc gcgatcgtga aggcgagcag
     1501 gaagaagaag acggagtagg cggcctgggc gacagcatgt gcctcgaagc ccaggatggt
     1561 gcccgagagc gcgccttcac ctgcgaagag cggcgcaatc gccatctcga aggacgtgcc
     1621 cgccgtacgg ggaatggcga ggcagggacc gatcgagaga tagatcagaa gcgtgaagag
     1681 gaaggcgaag gccggatgga cgcggttgcc gagcgccgtg aggccgcccg agcgcgcgac
     1741 agcggccacg ccgagaatcg ggaagagcac ggccgtgacg gcaaagcccg acatggcggg
     1801 cagggttgcg tcgcccgcaa gggcgccgag gaacggcggg aagatcagat tgcccgcgcc
     1861 gaagaacatc gagaagagcg tgaggccgat gaggcagcgc tccttgagag tgaagctttt
     1921 catagtgtgc tgtgattgag tgtctgtctg cgcgcgcgtc ctctcgatcg cgcgcctgac
     1981 cgcagccggc ctctttcgga cggcttttgc ggagatcacg aacaatagca cagggcggat
     2041 gaggctgaaa tcggggaaag cgttgagggg cgcggtttcg ccgcagcacg aggaggaagt
     2101 ccctggaagc gggatgcaaa ggcggaaggg aggagaaagc ggacaaaaga aaagtccctg
     2161 cagacttgca tctgcaggga ctcaagaatc tggggtggaa aatgggactc gaacccacga
     2221 caaccggaat cacaatccgg gactctacca actgagctat tcccaccagt ctttttgttt
     2281 tctcaagagc gtgtcgttct cgagagattt gaattgtaca ggaagttttt ggttttgcaa
     2341 ggggtgagga ctaaaaatac cgtaaatgcg gtacgttgaa ttgcgcatga ctgtggcgca
     2401 atgaacttta ttgttttgta acaaatgcga atgaccggcc gttttacacg cacccgtgcg
     2461 gatccgctga aggcggcggg cgcatgggct aaaatcacgg ctttccgttc accatcccat
     2521 atataaaagg agcattcatg gcccgaggcc ctcatctgag cgaactcgcc gccgagtggc
     2581 aggcgctggg cgcgaagccc cagcacgttc ggcgcatctt tcttgcctgg gcggggcttg
     2641 ccccgtggga ggccagaaag ggcgagagct ttccgaaggc cgtggccgag gcgctgcccg
     2701 acatcaggga gcgtcttgac cgcctctcgc atgcgctgcc cgttcgaagc gccgaggccg
     2761 agaccgtcaa gctcattctc gggcttgagg acggcgagtg cgtcgagtcg gtgttgctgc
     2821 ccaggcaggg actgtgcgtc tctactcagg tgggctgcgc cgtcgggtgc gtcttctgca
     2881 tgacgggcaa gggtggcctc gtgcgccagc tctcgagcgc cgagatcgtc gcgcagtact
     2941 gcgaggccag gcgggtgcgc agcgacgtca ggaagatcgt cctgatgggc atgggcgaac
     3001 cgagccacaa cctcgacgcc gtctgcgagg cggtggagtt cttcggcaac gtgtgcggac
     3061 tcgcgcacaa ggacatcgtg gtctcgaccg tgggcgacca caggctcttc gaccgcctgc
     3121 ccgcgctttc cgtgcgtccc gcgctcgcgc tcagcctcca caccacggac aacgcgctgc
     3181 gcaggaagct cctcaccaac tcgaaggaca ttcccgtcga gaccctcctc gagagaacgc
     3241 tcgactacgc cgatgagacg ggataccccg cccagatcga gtggacgctg atggcgggca
     3301 tcaacgacgc gcccgagcag gtcgagcgtc tggccgatct catcgaggga cgctacgcca
     3361 tggtgaactt catcgccgtc aatcccgtcg agggctcggg cttcaggagg cccgagtcgg
     3421 cccacatgca ggacctcatc acgatcctgc gcagacgagg taccgtcgcg acccttcgcg
     3481 acagtgcggc tcaggacatc gagggcggct gcggacagct ccgcgcacga gtcatcggca
     3541 tcaggaagga gcaggcctga cggtccgttg cttcggtttc agaggccgcc tgtgggcggc
     3601 cttttcttct ctgggaattg aggatggcat tcgcctttct tcgcagatgt tttgcttcgc
     3661 cggacaggtt ctcccgaagg agcccttcgc ctgtgttcat gcaccggcac ctctgcacgg
     3721 caggtcccgg tgacagggaa aatttcctgt gagtcaagct cttcctgggt ccctgacctt
     3781 tccctgtaac aggctttggc tgttattttt tgtgggatcg aattcttttc tcttgaaaga
     3841 ggggctgaaa actgcggagt ttctatccaa ttctggagtg gctcggttgt tctgatgcac
     3901 tctgcccgcc tgcatcagtc cggcgggcgc aactcctgaa aaccacaaag cccggacggc
     3961 tcttgcgctt ccgggcttca gtcattaggc tgcttttgca gatcagatct gttcgtagat
     4021 cttggaaagc tgtgcgaaga cggccttgtc ttcttcagtg gcttcgcgat aggctgcgtc
     4081 ggagaaggcg acgcctgcga tgtcggccgc agtgtagaaa cggagttttt cggtcacgcc
     4141 ggaattgctg ccgccgccga tctggaagcc gatcatcttg atttcgttgg tgggcgtaag
     4201 gccgcaggca taggggacga ccacgcggtc aaagccgtgg tatgcaaatt ccagggcctt
     4261 cttttccttg atcgcctgtg cgatcgtgtc aactgtcgtc atttcaattc tccttggtcg
     4321 gaaagttgcg tcgaatattt cggacatgtg ctttaagtat ctgctcaatt gagtgtaaat
     4381 gacatgactt ggctcaaaag attgatatgt cgatacaaaa gaatgggtga cgatcggcga
     4441 agaagaggtg aaaatgcagg gttttcgagg gctttttgcg ggagatgttt tgcagtataa
     4501 gctgaacggc gtgcgtgttc ctgccggcac tcaggcagtt gagctgatgc tcggtacgtc
     4561 gtgcctcagc ggctcgcaac cttccgggcg gcgatcatcc cgttgatgat gcagatcgtg
     4621 agggtgttgg agccgaggcg gacggtgccg tggacgccac ccatgcagtg ggcttgggcg
     4681 aaaagcgcgt gacgcaccaa gggccgtcag tccgggctct aagacatttt tccaaggtcg
     4741 cgatgtaata cctcaaggtt cgctcacttt cacatgacgc ttcgaaaggc ttctgctaga
     4801 gttcaatcac ggtcttctga gagttgaccg aaacggaaac gcccggcagg tccgggctcg
     4861 ccttcagacg aacccgaggg gttctcgtca aaggcgacct tctccagaac agtcatccga
     4921 atcctcttcg gcctgatgcg gcatcctcgg ccgcacggac tgctctgatg tttctgcgaa
     4981 acggacggac aacgtccccg tccgacaatc ccaaaaactg tttttccgca atcaacatcc
     5041 ggagaaccca aatgaatctc tcccgacttt tcgcacccat cgccctcgtg gccgccattg
     5101 ccctgtccgg catttccggc agcgccctcg ccgacacgac gcttcgcatc ggcgtcacgc
     5161 ccgtgccgca cgccgacatc gtcaacttca tcaagccgac gcttgagaag gaaggcgtga
     5221 agctcgaggt ggtggaattc aacgactaca tccagccgaa cctcgcgctc gatgcgggcg
     5281 aacttgacgc caactatttc cagacgattc cgtatctgga ggaaatggtc aagtcccgcg
     5341 gcctcaacct gcacatcctc gtctccgtgc atcttgagcc gatggccctc tactccaaga
     5401 aggtgaagag cctcgccgac attccggcag gcgccaaggt ggccgtgccg tccgacccga
     5461 cgaacgaggg tcgtgcactc aaggtgctcg agcacgcggg cctcatcaag ctcaaggacg
     5521 gcgcgacgct cctctccggc atcggcgaca tcgtctcgaa tccgaagaag ctgaagttcg
     5581 tcgagcttga gcccgctctt ctgccgcgcg ccctcgacga cgtgacggcc gccgtcatca
     5641 acgccaatta cgcgctcgaa gcgggcttca acccggcgaa gaacgccatt gcgctggagg
     5701 aaaagagttc gcccttcggc aacgtcgtcg cgatccgcga aggcgacgac aagaaggagg
     5761 ccttccagaa gctcaagaag gcgatcacct ctcctgaagt gaagaagttc atcgaggaga
     5821 agtatcaggg cggcgtgctt cccgtcttct gagaattgcg ttagtctgaa gtgaaatgca
     5881 aggcaaggcc ggtccgggtt cgacccgtgc cggcctttgc gtcgatctgg agaatgatca
     5941 tgacccgtac cgacaagctc gtcgccgccc actggggtgt cgcccgcgtc cgcatgacgg
     6001 acggccgtcc cgcgctcgtt ccgatcgtcg aggacatgag gccctcggaa cgcatggccg
     6061 actatctggc ggggcgtgat ggcgccgaac gccttctcgc tccgaaggtc cgtgaaggct
     6121 atctcagggc ctgccgcgag ggacgcgatc ccgccctgac ccgtgagggc cgcggcaggg
     6181 aggccttcgt cgaggtcgaa tgggaggagg cgctcgagct tgccgcccgc gcgatcgaga
     6241 ggacgtatgc ggaccacgga ccctcggccg tctggggccg gtcctacggc tggaagaaca
     6301 cgggcgaggt caacaatccg atcggactcc tgagaaggct gctgatgctc aagggcggat
     6361 tcgtgcagac cttcaactcc tactccacgg ccgccatcgc cgccatacag aaggtgatcg
     6421 caggaagcgg agaccccgac gtgccgcctg cggccgaggt gctcgagcat gccgagcgcg
     6481 tcattctctg gggtgcggat ccggtcgtca cgaacgacat cgcctggacc acgacgctgc
     6541 acgacacggc gaaggtcttc gaggcgatga gaacgaagga gggactcgag gtcgtcgccg
     6601 tcaatcccgt gaagcccgag acgctcgacg tcacgggcgg gcgctggatc gcgccgcttc
     6661 ccgggacgga cgccgcgttc gcgctcggcc tcgtgcacac gcttctggcg acgggcaggg
     6721 ccgaccgtca ttttctcgag acccgcacga cgggcttcga aacgctcgag gcctgcctca
     6781 acggacgtct tgacggcgtc gtccgcgacg ccgcctgggc ggccggcatc tgcgggctcg
     6841 acgagaagac gatcagggat ttcgctgagg agcttgcggc gcaccggacg atgatcatgc
     6901 tcggctgggg gccccagagg gcccgctacg gcgagtcgac ggtctgggcc gtctgggcgc
     6961 tcgccgcagc gctcggccag atcggcggac gaggtacggg catcggcacg cgctaccact
     7021 actcttcggg cggcgcgggc gcgtcggcgg gcaggggcct tccgggcctt cccgtcaacg
     7081 tggcgcc
//
LOCUS       DBMDTI010000094         7083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2790_length_7083_cov_3.810370, whole genome shotgun sequence.
ACCESSION   DBMDTI010000094 DBMDTI010000000
VERSION     DBMDTI010000094.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2790_length_7083_cov_3.810370"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="ACZPB6_05795"
     CDS             <1..669
                     /locus_tag="ACZPB6_05795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1184016.1"
                     /translation="PLRVKGIEKFEGTQLHYSCKDPSVFEGKNVVICGGGDSALDWTL
                     NLVGRAESVVLVHRRDGFRAQAASVAKMKELCENWEMQFEVGQVTGFNEADGKLTEVL
                     VAGADGVTRRIPLDHLLVFFGLQPKLGPIENWGLELDRKQIVVDTARFETNIPGIFAV
                     GDINTYPGKKKLILSGFHECALAAFAACDYVFPEKRVFLQYTTTSPKLHKVLGVETPN
                     FDED"
     gene            complement(789..1877)
                     /gene="apbC"
                     /locus_tag="ACZPB6_05800"
     CDS             complement(789..1877)
                     /gene="apbC"
                     /locus_tag="ACZPB6_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HIR1184017.1"
                     /translation="MTISTEAIKSALSALIDPVTGTDYVSGKMFKSAETDENGNVKVF
                     IELGYPAKHRAEAVGQAVGEAVRAAGAASVEVSVTQNIIAHKVQGTLRVMPGVKNIIA
                     VSSGKGGVGKSTVSANLALALAHEGARVGVLDADIYGPSQPKMLGASGQPVSADGKTM
                     EPMESLGLQINSIGFMIEEDDPMIWRGPMAAGALQQLITQTNWHDLDYLIVDMPPGTG
                     DIQLTLSQQVPLTGAVVVTTPQDIALIDAKKGLRMFQKVNVPILGIIENMSVFICPHC
                     GEVEHIFGEGGAKRMSEQYGVPLLGELPLSAKIREQADSGLPTVAAEPDGKEALMYRE
                     MAMKVAGALARITKDYTAKMPGIRVVND"
     gene            2076..4133
                     /gene="metG"
                     /locus_tag="ACZPB6_05805"
     CDS             2076..4133
                     /gene="metG"
                     /locus_tag="ACZPB6_05805"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR1184018.1"
                     /translation="MSQRNLFVTTALPYANGAFHMGHIMEYIQADIWVRHERMSGNKV
                     HFVGADDVHGAPIMIAAQKRGITPQAFVAEIAAGRKQYLDGFHISFDNWHSTDAVENH
                     ELSQDIYRRLVAAGLIYTKEIEQYYDTVKGMFLADRFIKGTCPRCGATDQYGDNCEKC
                     SAVYSPMEVVNPYSTLSGSRPEIRKSTHYFFKLSDPQCVQFLRDWTNGKGKDGLPRVQ
                     QEVLAKDSEWLGKDGNLSDWDISRDEPYFGIAIPDAPGKYFYVWLDAPVGYLASLKNY
                     CDKQGLDFEGILSDEKTEQIHFIGKDIIYFHTLFWPAMLHFAGKPFRVPDHVNAHGFM
                     TVNGEKMSKSRGTGISPLQYLELGMDAEWLRYYLAAKMNSRVEDVDFTKSDFAQRVNS
                     DLIGKYVNIASRAAGFIFKKFEGRVDDSAMQDPLLEAIRSRAEEVKGFFEIREFARAT
                     RLVMELADKINEFVDQEKPWELAKNPEARAELHRVSSIALEGFRLLTLYLKPVLPATA
                     TRVEDFLSIEPLTWASVSVPLSSASPIKAFKHLMQRVDMEKQLDVLVPEKAPEPEPVL
                     PGGEAIAPECTIDDFTKIDLRVAKIVKCEPVEGSTKLLRLTLDLGEGRTRNVFSGIAS
                     AYKPEQLEGRMTVVIANLAPRKMRFGVSEGMVLAASDAGDKSLGLFVLEPHSGAIPGM
                     RIR"
     gene            4265..6013
                     /locus_tag="ACZPB6_05810"
     CDS             4265..6013
                     /locus_tag="ACZPB6_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR1184019.1"
                     /translation="MSFKDMAREFMHHVPLAKFHPVLLTCLKEYDSRMLSRDISAGLT
                     VGMVALPLAMAFGIASGVPPESGLYTAIIAGFLISLLGGCRVQIGGPAGAFVVIIYGI
                     IAQYGLSNLMLATLGSGVILFLMGLFKIGGFIKFIPVSIVIGFTNGIAVMIGLQQVKD
                     FLGLTVAKMPADFFGQVNTIINNIGTINPWAFGLALASFLIVKFWPKGYQMSGPQWKR
                     WLAHLPGTVVVLVLSTIIVTVFGLPVETIGSKFGGIPQSLPSLQIPDLEWDTAKQLLG
                     PMITIAVLGSIESLLCARVADTMTDDRHDPNQELMGQGVANMIVPFFGGIPATGTIAR
                     TVTNIKSGAFSPVAGMVHAITLLVVILAAAPLASNIPLCALAAILVFVAWNMGNWREF
                     LRLRSMTLSYKATLLITFFLTVIFDLTVAVQFGLVVACVFFLMRMNNLTVVEPVTLPF
                     NVPDTVEAWHIKGALFFGSVSKLEHVTDPARITSASAARIVILDFTDLLNIDNSAMDQ
                     IEVYARALHRHNHELIIAGASGHPLAQLKRTNIAQRLGANLVPNMEFAFARAEIILKE
                     QDEKAAAKRALLGGEA"
     gene            complement(6094..6969)
                     /locus_tag="ACZPB6_05815"
     CDS             complement(6094..6969)
                     /locus_tag="ACZPB6_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR1184020.1"
                     /translation="MTDLFTAHRGVDTQTEEKILARLYGEPLEECPDGLYIPPEALRV
                     FLASFEGPLDLLLYLIRKQKFDVMDIPMAELTDQYMRYVDLVRESDLELAAAYLVMSA
                     TLMQIKSRLLLPSVRTAEGTEPEDPRAELMRRLEEYERIRSAALKLQAMPRIDRDFRV
                     AEVERCVPVVALQPDVTPEMLASAWAAVAARLKLRDRHKVSRQELSVREHMTALLKRL
                     QTEPFVTLEGLWNGTRDRETMTVWFLALLELAKEGMIILTQGAPYAPLYATLPEDAGT
                     LELRCPDPDPLVLTS"
BASE COUNT         1270 a         2480 c         2007 g         1326 t
ORIGIN      
        1 ccgctccgcg tcaagggcat cgagaagttc gagggcacgc agcttcacta cagctgcaag
       61 gatccctccg tctttgaagg caagaacgtc gtcatctgcg gtggcggcga ctccgccctc
      121 gactggacgc tcaacctcgt gggccgcgcc gaaagcgtcg tgctcgttca ccgccgcgac
      181 ggcttccgcg cccaagccgc ctccgtcgcc aagatgaagg aactgtgcga gaactgggag
      241 atgcagttcg aagtgggcca ggtcacgggc ttcaacgaag ccgacggcaa gctcaccgaa
      301 gtgctcgtcg cgggcgccga cggcgtcacg cgccgcatcc cgctcgacca tctgctcgtc
      361 ttcttcggtc tgcagcccaa gctcggcccg atcgagaact ggggcctcga gctcgaccgc
      421 aagcagatcg tcgtcgacac cgcccgcttt gagacgaaca tcccgggcat cttcgccgtg
      481 ggcgacatca acacctaccc cggcaagaag aagctcatcc tctccggctt ccacgaatgc
      541 gccctcgccg ccttcgcggc ctgcgactac gtcttcccgg aaaagcgcgt gttcctgcag
      601 tacacgacca cgtcgccgaa gctccacaag gtactcggcg tcgagacgcc gaactttgac
      661 gaagactgat cgtctttgag tccaaggact ccatggacag aggggacggc cctgcggccg
      721 tcccctcttg ctttttcgcc tgcgaagacc cgcgctcaac gcgggccttc gacgccgttt
      781 tctttcgatc agtcgttcac gacgcggatg ccgggcatct tggccgtgta gtccttcgtg
      841 atgcgggcga gagcgccggc gaccttcatc gccatctcgc ggtacatcag ggcctccttg
      901 ccgtcgggct cggcggcgac cgtgggcagg ccgctgtcgg cctgttcgcg gatcttggcc
      961 gaaagcggaa gctcgccgag aagcggcaca ccgtactgct cgctcatgcg cttggcgccg
     1021 ccttcaccga agatgtgctc gacttcgccg cagtgcgggc agatgaagac ggacatgttc
     1081 tcgatgatgc cgaggatggg cacattcacc ttctggaaca tgcgcaggcc cttcttggcg
     1141 tcgatgagcg cgatgtcctg cggggtcgtc acgacgacgg cgcccgtgag cggcacctgc
     1201 tgcgagagcg tgagctggat gtcgcccgtt cccggaggca tgtcgacgat caggtagtcg
     1261 aggtcgtgcc agttcgtctg ggtgatgagc tgctgcaggg cgcctgcggc catcgggccg
     1321 cgccagatca tcggatcgtc ctcctcgatc atgaagccga tcgagttgat ctgcaggccg
     1381 agggattcca tcggctccat cgtcttgccg tcggcgctca cgggctggcc ggaggcgccg
     1441 agcatcttgg gctgggacgg accgtaaatg tccgcatcaa gcacgccgac gcgggcgcct
     1501 tcgtgcgcaa gcgcgagcgc gaggttggcg gagaccgtgg acttgccgac gccgccctta
     1561 ccggagctca ccgcaatgat attcttgacg ccgggcatga cgcgaagcgt gccctgcacc
     1621 ttgtgcgcga tgatgttctg cgtgacgctc acctcaacgg aggcggcacc tgcggcgcgg
     1681 acggcctcgc ccacggcctg accgacggct tcggcacggt gcttggcggg atagccgagc
     1741 tcaatgaaca ccttgacgtt gccgttctcg tcggtttcgg cgctcttgaa catcttgccg
     1801 gacacgtagt cggtgccggt gacagggtcg atcagcgcgg aaagggcgct cttgatcgct
     1861 tccgtggaaa tggtcatggg attcttctcc gttgatgtcc gcgcgcatcg cgcgtgtggt
     1921 tatgcggatt ttacgcgccg atcctttgat tttcctgaaa aggcccggac tggaaaggct
     1981 ctccaagccc cctcgggttt tcgctacgct aaaataactg attgcttcgg attttccgaa
     2041 gcgcccttca tttcaacttc ggatttaaca cgacgatgag tcagcgcaat ctttttgtta
     2101 cgaccgcctt gccttacgcg aacggcgcct tccacatggg ccacatcatg gagtacatcc
     2161 aggctgacat ctgggtgcgt cacgagcgca tgtccggcaa caaggtccac ttcgtcggcg
     2221 ccgacgacgt gcacggcgcg ccgatcatga ttgccgccca gaagcgcggc atcacgccgc
     2281 aggccttcgt cgccgaaatc gccgcaggcc gcaagcagta cctcgacggc ttccacatca
     2341 gcttcgacaa ctggcacagc accgacgccg ttgaaaacca cgagctcagc caggacatct
     2401 accgccgcct cgtcgccgcc ggtctcatct acacgaagga gatcgagcag tactacgaca
     2461 cggtcaaggg catgttcctc gccgaccgct tcatcaaggg cacgtgtccg cgctgcggcg
     2521 cgaccgacca gtacggcgac aactgcgaaa agtgttccgc cgtctactcc ccgatggaag
     2581 tcgtgaaccc gtactccacg ctttcgggct cccgtccgga aatccgcaag tccacgcact
     2641 acttcttcaa gctctccgac ccgcagtgcg tccagttcct ccgcgactgg accaacggca
     2701 aaggcaagga cggcctgccg cgcgtgcagc aggaggtcct cgccaaggac agcgaatggc
     2761 tcggcaagga cggcaacctc tcagactggg acatctcccg cgacgaaccg tacttcggca
     2821 tcgccattcc cgacgctccg ggcaagtact tctacgtctg gcttgacgcc cccgtcggct
     2881 acctcgcctc gctcaagaac tactgcgaca agcagggcct cgacttcgaa ggcatcctct
     2941 ccgacgaaaa gacggagcag atccacttca tcggcaagga catcatctac ttccacacgc
     3001 tcttctggcc cgccatgctg cactttgccg gcaagccctt ccgcgtgccc gatcacgtca
     3061 acgcccacgg cttcatgacc gtcaacggcg agaagatgtc caagagccgc ggcaccggca
     3121 tctcgccgct gcagtacctc gagctcggca tggacgccga atggctccgc tactaccttg
     3181 ccgccaagat gaactcccgc gtcgaggacg tcgacttcac gaagtccgac ttcgcccagc
     3241 gcgtcaactc cgacctgatc ggcaagtacg tcaacatcgc cagccgcgcc gcgggcttca
     3301 tcttcaagaa gttcgagggc cgcgtcgacg attccgccat gcaggatccg ctgcttgaag
     3361 ccatccgcag ccgcgccgag gaagtcaagg gcttcttcga aatccgcgag ttcgcccgcg
     3421 ccacgcgcct cgtaatggag ctcgccgaca agatcaacga gttcgtcgat caggaaaagc
     3481 cgtgggagct cgccaagaac cccgaggcca gggccgaact gcaccgcgtc tcctcgatcg
     3541 ccctcgaagg cttccgcctg ctcacgctct acctcaagcc cgtgctgccc gccacggcca
     3601 cccgcgtcga ggacttcctc tcgatcgaac ctctcacctg ggcgagcgtc tccgtgccgc
     3661 tctcctccgc gagcccgatc aaggccttca agcacctgat gcagcgcgtc gacatggaaa
     3721 agcagcttga cgtcctcgtc cccgaaaagg ctcccgagcc cgaacccgtc ctgcccggcg
     3781 gcgaagccat cgctcccgag tgtacgatcg acgacttcac gaagatcgac ctgcgcgtcg
     3841 ccaagatcgt caagtgcgag cccgtcgaag gctccacgaa gcttctgcgc ctcacgctcg
     3901 atctgggcga aggccgcacc cgcaacgtct tctccggcat tgcaagcgcc tacaagcccg
     3961 agcagctcga aggccgcatg acggtcgtga tcgccaacct cgccccgcgc aagatgcgct
     4021 tcggcgtctc cgaaggcatg gttctcgccg cctccgacgc cggcgacaag tcgctcggcc
     4081 tcttcgtgct cgaaccgcac tccggcgcta ttcccggcat gcgcatccga taagatcgac
     4141 ttatttgcgc aataggcctg attttttggc cattgcttgc gattgagcaa aggcatcctc
     4201 ttcctaagga ggatgccttt tcttacaatt agacgttttt ccctcacctc atccggtttt
     4261 cgacatgagc ttcaaagaca tggcccgcga attcatgcac cacgtgccgc tggccaaatt
     4321 ccatccggtg ctcctcacct gcctgaagga atacgacagc cgcatgctgt cgcgcgacat
     4381 ctccgccggc ctcaccgtgg gcatggtggc cctgccgctc gccatggcct tcggcatcgc
     4441 ctccggcgtg ccgcccgaat cgggcctcta caccgccatc atcgctggct tcctcatctc
     4501 gcttctcggc ggctgccgcg tccagatcgg cggtcccgcc ggcgccttcg tcgtcatcat
     4561 ctacggcatc atcgcacagt acggcttgag caacctcatg ctcgccacgc tcggctcagg
     4621 cgtcatcctc ttcctgatgg gcctcttcaa gatcggcggc ttcatcaagt tcatccccgt
     4681 atcgatcgtg atcggcttca cgaacggcat tgcggtcatg atcggcctgc agcaggtcaa
     4741 ggacttcctc ggcctcaccg tcgccaagat gcccgccgac ttcttcggcc aggtcaacac
     4801 gatcatcaac aacatcggca cgatcaatcc gtgggccttc ggccttgcgc ttgcgagctt
     4861 cctcatcgtc aagttctggc ccaagggcta tcagatgagc ggcccgcagt ggaagcgctg
     4921 gctcgcccac ctgcccggca ccgtcgtcgt cctcgtgctc tccacgatca tcgtcacggt
     4981 cttcggcctt cccgtcgaaa cgatcggctc caagttcggc ggcattccgc agtccctccc
     5041 gagcctgcag atccccgacc tcgagtggga caccgccaag cagcttctcg gcccgatgat
     5101 cacgatcgcc gtgctcggtt ccattgaaag cctgctgtgc gcccgcgtgg ccgacacgat
     5161 gaccgacgac cgccacgacc ccaatcagga actgatgggc cagggcgtcg ccaacatgat
     5221 cgttccgttc ttcggcggca tccccgccac cggcacaatc gcccgtaccg tcacgaacat
     5281 caagtccggc gccttctcgc ccgtcgcggg catggtccac gccatcacgc ttctcgtcgt
     5341 gatcctcgcc gccgccccgc ttgcgagcaa catcccgctc tgcgcgcttg ccgccatcct
     5401 cgtcttcgtg gcctggaaca tgggcaactg gcgcgagttc ctgcgcctgc gctcaatgac
     5461 gctctcctac aaggcgacgc ttctcatcac gttcttcctg accgtgatct tcgacctgac
     5521 ggtcgccgtc cagttcggcc tcgtcgttgc ctgcgtcttc ttcctgatgc gcatgaacaa
     5581 cctgacggtc gtcgaacccg tcacgcttcc gttcaacgtg cccgacacgg tcgaggcctg
     5641 gcacatcaag ggcgccctct tcttcggctc cgtctccaag ctcgagcacg tgaccgatcc
     5701 agcccgcatc acgtcggcct ccgcagcccg catcgtgatc cttgacttca ccgacctgct
     5761 caacatcgac aactccgcga tggaccagat cgaagtctat gcacgcgccc tacaccgcca
     5821 caaccacgag ctgatcattg cgggcgcctc cggccacccg ctcgcccagc tcaagcgcac
     5881 caacatcgcc cagcgtctcg gtgcaaacct cgtgccgaac atggagttcg ccttcgcccg
     5941 tgccgaaatc atcctcaagg agcaggatga aaaggccgcc gccaagcgcg ctctcctcgg
     6001 cggagaagcc taaacagcct ccacgctcag gcaaagacaa aaaccccgtc aggctctccg
     6061 cccggcgggg ttttgttttg tccgttcacc gtctcagctc gtcagcacga gcggatcggg
     6121 atcggggcaa cgaagctcaa gcgtgcccgc atcctcgggc agcgtggcgt agagcggcgc
     6181 gtagggcgct ccctgagtga ggatgatcat tccctccttg gcaagctcga ggagcgccag
     6241 gaaccacacg gtcatcgtct cgcggtctcg agtgccgttc cagagcccct ccagcgtcac
     6301 gaagggctcc gtctgcaggc gcttcagaag ggccgtcatg tgctccctca ctgaaagctc
     6361 ctgcctgctc accttgtgcc ggtcccgaag cttgagacgg gcggcgacgg cagcccaggc
     6421 cgatgcgagc atctcgggcg tcacatcggg ttggagcgcc accacgggca cgcagcgctc
     6481 cacctcggcg acacggaagt cccggtctat cctcggcatg gcctgcagct tgagcgccgc
     6541 agaacgaatc cgctcgtact cctcaagacg gcgcatgagt tccgccctcg gatcctcggg
     6601 ttccgtcccc tcggccgtcc tcacggaagg cagcagaagc ctgctcttga tctgcatgag
     6661 cgtcgcggac atcacgagat aggctgcggc cagctccaga tcgctctccc tcaccagatc
     6721 cacgtagcgc atgtactggt cggtaagctc cgccatcggg atgtccatga cgtcgaactt
     6781 ctgcttgcgg atgaggtaga gaagcaggtc gagcggtccc tcgaagctcg ccagaaagac
     6841 gcgcagcgcc tcgggtggaa tgtacagccc gtcgggacac tcctccagcg gctcgccgta
     6901 gaggcgtgcg aggatcttct cctcggtctg cgtgtcgacg cctcggtgcg cggtgaaaag
     6961 gtcggtcatg aagggacggg ttcctgcaga agatgaaggc ccgccgcatc agggctgacc
     7021 tctcgggcgt cggggagaat accacaacgc acgccctcgg gtcccgccct gcggctgcct
     7081 gcg
//
LOCUS       DBMDTI010000095         6824 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2892_length_6824_cov_3.754723, whole genome shotgun sequence.
ACCESSION   DBMDTI010000095 DBMDTI010000000
VERSION     DBMDTI010000095.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2892_length_6824_cov_3.754723"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="ACZPB6_05820"
     CDS             <1..462
                     /locus_tag="ACZPB6_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1184021.1"
                     /translation="APSARALDGADVTTDNAVKLRDERGRTLSGKLDYVAQSVNPKTS
                     TRLMRVRLDEGSGLLPGDFVHVDLQIGVRENAVRIPQKAVRQEPDGTYTVFVYQDGKA
                     RLRPVKLDRWDGPDWIATSGLATGDRVITNQLLKLRDGSAVQPAEASKTAK"
     gene            474..3575
                     /locus_tag="ACZPB6_05825"
     CDS             474..3575
                     /locus_tag="ACZPB6_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1184022.1"
                     /translation="MLSQFCIRRPIFATVLSIIIVLAGLIALRVLPLSQYPDISPPTV
                     RISTTYDGADAQTLARTVAAPIEDQLSGITGLLYYTTNIRSNGDMSISCVFDVGTDAN
                     DAMLEINNRVRTAERRLPDVVRNQGVSVRKRSEENLLMVALFSPDGSMAATDLADYAN
                     LNIVDELKRLPGIGDVSVFGNAQSAMRIWLDPEKMGLMGITIKDVENAIEAQNAQRSA
                     GRVGTAPTVSEQQLYYTITTPGQLLTAEEFADIIVKADGPDKLVRMKDIATTEIGKRS
                     YEFRVDINGTQGVNVGVYLQTGANAMAAAEAVKARIVELAAQFPENKIDYIITDDTSI
                     FVGASLNEVYHTLFEAGILVLLVVFVFLQNWRATLIPMLAVPVSLIGTMAGLWLFGFS
                     LNTLTLFAMTLSIGIVVDDAIVVLENVERLMRTEGLGPYEAAQKAMKEVSGALVAIVL
                     VLASVFIPVAFLGGIAGELYRQFAVTVALSVVLSGFVALTLTPALCAILLKERKPEER
                     KNRFFQAFDRGLASFTLRFLKLVKAALRHRVITAGLLVAVTAGCWQMLEHTPTSFIPR
                     EDQGIVRISVQLPEGAAFPRTEAVSTEILEHLKKNDAVQSVVTMVGYDTMSGDVRSNA
                     STFILKLKHWDDRKETAEEIQKDLQKYVASHPDIKGVAALPAAIKGLGSTNGFSGYIL
                     SHGNDNPLVLQNVAENFLDALQDRPELTGLRSYLTADTPQLKLYVDQTKAIALGVDVD
                     DIYDTISHLMGSKYVNDFTRNGKIYRVVVQAAPQFRSTPEDIGSGYVRSSSGEMVPIS
                     ALVRTERTSGAAALARMNGYLAAQFSGAAAQGVSSGDAIRIVEETAREVLPEGYTIEW
                     VGQAYHEKRIGASSATAFGFGILMMFLILAALYERWSLPIAVVLAVPYAFLGAMTAIW
                     LRGTPNDIYFQIGLLVLIGLTAKNAILIVEFAAQKMEEGMGPFDAAIEAAGLRFRPIL
                     MTSFAFVLGVVPLIIATGAGAGARHSMGTGVFGGMLAATFISTIFVPVFFTWFARKSS
                     KKR"
     gene            complement(3659..4447)
                     /locus_tag="ACZPB6_05830"
     CDS             complement(3659..4447)
                     /locus_tag="ACZPB6_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1184023.1"
                     /translation="MLELKNVTKTFFAGTANEKKALRGVSFTLEDGDFCTVIGSNGAG
                     KSTTLNAIAGAFPIDSGSILIDGVDVSRMPEYKRAAFIGRVFQDPMRGTAAGMMISEN
                     LAIADRRGRSQTLRWSDRADRTAYFTELVKMLGLGLENRMTAHVGLLSGGQRQALTLL
                     MATLVRPKLLLLDEHTAALDPKTAAKVLELTEKLVTEQQLTTLMITHNMRDALRYGNR
                     LIMMHDGRPILDVRGEEKAKLTAVDLLQFFEKAGDGVSDKMLLG"
     gene            complement(4478..5362)
                     /locus_tag="ACZPB6_05835"
     CDS             complement(4478..5362)
                     /locus_tag="ACZPB6_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1184024.1"
                     /translation="MLDLILSTVGQGLQWSVLALGVFLTFRILDIADLSVEGSFPLGA
                     AVAATAITAGLGLPAAFVLALVAGSLAGGVTGLLTTKLRIPALLAGILTMIGLYSVNL
                     HVMGKSNVGLLQNETVFTILENSLGLSVAMSGLVVGLVFAVAIAVVIYWFFGTELGTA
                     VRATGFNPQMARAQGINTNTMVVLGLVISNGLVALSGATVAQANGFADVGMGVGTIVI
                     GLASVIIGEVLFSPKTFKTSLAAVILGSIIYRLVIAFVLEMGMPPNDLKLFTAVLVAI
                     ALALPLIKEKMAGLRARA"
     gene            complement(5533..6525)
                     /locus_tag="ACZPB6_05840"
     CDS             complement(5533..6525)
                     /locus_tag="ACZPB6_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HIR1184025.1"
                     /translation="MQISKITRRSAIALSVAAALTLAGCGDKEPAKSELVPVGIVQLV
                     EHSALDACTKGVKDALAERGYKDGVNIKIDFQNAQGDQSNLHNIATRFVSNKDKLIFA
                     VATPAAQAVATTAKGTPIVATAITDFVAAKLVKSDEQPGGNVTGVSDLGPIEAQLELL
                     LKLVPDAKAVGTIYNSSEINSKYQVDIFKKAAEKRGVQVLEATVSNVNDIQQAAASLN
                     GKVQGMWLPTDNVLASAIPALAKVANPSKIPVIAGERGMTEAGCLGSISVDYYEIGRM
                     TGQMGADILDGKKKPAEMPVQHVANGTPVFNMKTAAAIGITIPDELKKGAVLFQ"
BASE COUNT         1249 a         2218 c         2107 g         1250 t
ORIGIN      
        1 gcgccgtccg cacgtgcgct cgacggcgcc gatgtgacga ccgacaatgc ggtcaaactc
       61 cgcgacgagc gcggccgcac gctctcgggc aaactcgact acgtcgccca gtccgtcaac
      121 ccgaagacga gcacgcgcct catgcgcgtt aggctcgatg aaggctccgg acttcttccc
      181 ggcgacttcg tgcacgtgga ccttcagatc ggcgtacgag aaaacgccgt gcgcatcccg
      241 cagaaggccg tgcgccaaga acccgacggc acttacacgg tcttcgtgta ccaggacggc
      301 aaagccaggc tccggcccgt caagctcgac cgctgggacg gtcccgactg gatcgccacc
      361 tcgggcctcg ccacgggcga ccgcgtgatc accaaccagc ttctcaagct tcgtgacggc
      421 tccgccgtgc agcccgccga agcttccaag acggccaagt aggatcctgc gccatgctca
      481 gccagttctg catccgccgc ccgatcttcg cgacggtcct ctcgatcatc atcgtgctcg
      541 cgggcctcat cgcgctgcgc gttcttccgc tctcccagta tccggacatc tcgcctccga
      601 cggtgcgcat ctcgaccacc tacgacggcg ccgacgccca gacgctcgcc cgcaccgtgg
      661 ccgctccaat cgaggaccag ctctcgggca tcacaggcct gctctactac acgacgaaca
      721 tccgaagcaa cggcgacatg tcgatttcct gcgtctttga cgtcggcacc gacgccaacg
      781 acgccatgct cgaaatcaac aaccgcgtcc gcacggccga acgcagactg ccagacgtcg
      841 tgcgcaatca gggcgtgagc gtgcgaaagc gctccgagga aaacctcctc atggtcgcgc
      901 tcttctcgcc cgacggcagc atggccgcca ccgatctggc cgactacgcg aacctcaaca
      961 tcgtcgacga actcaagcgc ctgcccggca tcggcgacgt gagcgtcttc ggcaacgcgc
     1021 agtccgccat gcgcatctgg ctcgatcccg aaaagatggg cctcatgggc atcacgatca
     1081 aggacgtcga aaacgccatc gaggcccaga acgcgcagcg aagcgcgggc cgcgtgggca
     1141 cggcgcccac agtgtccgag cagcagctct actacacgat cacgacgccc ggtcagctcc
     1201 tcaccgccga ggagttcgcc gacatcatcg tcaaggctga cggtcccgac aagctcgtgc
     1261 gcatgaagga catcgccacg accgaaatcg gcaagcgctc ctacgagttc cgcgtcgaca
     1321 tcaacggcac gcagggcgtc aacgtcggcg tctacctgca gacgggcgcc aacgccatgg
     1381 cggccgccga ggccgtcaag gcccgcatcg tcgagctcgc cgcccagttc cccgaaaaca
     1441 agatcgacta catcatcacc gacgacacgt cgatcttcgt gggcgcctcc ctcaacgagg
     1501 tctaccacac gctgttcgag gcaggcatcc tcgtgctcct cgtcgtcttc gtcttcctgc
     1561 agaactggcg cgccacgctc atcccgatgc tcgcagtgcc cgtgtcgctc atcggcacga
     1621 tggcgggcct ctggctcttc ggcttctcac tcaacacgct gacgctcttc gcgatgacgc
     1681 tctcgatcgg catcgtggtc gacgatgcca tagtcgtgct tgagaacgtc gagcgtctca
     1741 tgcgcaccga gggcctcggc ccctacgagg cggcccagaa ggccatgaag gaggtctcgg
     1801 gcgcactcgt cgccatcgtg ctcgtgctcg cctccgtctt catccccgtc gccttcctcg
     1861 gcggcatcgc aggcgagctc taccgccagt ttgccgtgac ggtcgccctg tccgtcgtgc
     1921 tctcgggctt cgtcgccctg acgctcacgc cggcgctctg cgcgatcctt ctcaaggaaa
     1981 ggaagcccga agagcgcaag aacaggttct tccaggcctt cgaccgcgga ctcgcgtcct
     2041 tcacgctcag gttcctcaag ctcgtcaagg cggcgctgcg ccaccgcgtg atcaccgcag
     2101 gcctgctcgt cgccgtgacg gccggctgct ggcagatgct cgagcacacg ccaacctcct
     2161 tcattccgcg cgaggaccag ggcatcgtgc gcatctcggt ccagctcccc gaaggcgcgg
     2221 ccttcccccg caccgaggcc gtctcgaccg aaatcctcga gcacctcaag aagaacgacg
     2281 ccgtgcagag cgtcgtgacg atggtgggct acgacacgat gagcggcgac gtccgcagca
     2341 acgcatcgac cttcatcctg aagctcaagc actgggacga ccgcaaggag accgccgagg
     2401 agatccagaa ggacctccag aagtacgtcg cctcccaccc cgacatcaag ggcgtggctg
     2461 cgctgcctgc ggccatcaag ggccttggct ccacgaacgg cttctcgggc tatatcctct
     2521 cgcacggcaa cgacaatccg ctcgtgctgc agaacgtcgc cgaaaacttc ctcgacgccc
     2581 ttcaggaccg ccccgagctt acgggcctgc gcagctacct gacggccgac acgccccagc
     2641 tcaagctcta cgttgaccag accaaggcga ttgcgctcgg cgtggacgtc gacgacatct
     2701 acgacacgat ctcccatctg atgggcagca agtacgtcaa cgatttcacc cgcaacggca
     2761 agatctaccg cgtggtggtt caggccgctc cgcagttccg ttcgacgccc gaggacatcg
     2821 ggtcgggcta cgtgcgctcg agctcgggtg aaatggtgcc catctcggcg ctcgtgcgca
     2881 ccgagcgcac gtccggtgcg gcggccctcg cccgcatgaa cggctacctc gccgctcagt
     2941 tcagcggcgc ggccgctcaa ggcgtctcgt cgggcgacgc catcaggatc gtcgaggaaa
     3001 ccgcccgtga agtgcttccc gaaggctaca cgatcgaatg ggtcggtcag gcctaccacg
     3061 aaaagcgcat cggcgcctcg tccgccacgg ccttcggctt cggcatcctg atgatgttcc
     3121 tcatcctcgc cgccctctac gagcgttggt cgctgccgat cgccgtcgtg ctggccgtcc
     3181 cgtacgcctt cctcggcgcc atgaccgcca tctggctgcg cggcacgccc aacgacatct
     3241 acttccagat cggtctactc gtgctcatcg gcctcacggc caagaacgcc attctgatcg
     3301 tggagttcgc ggcccagaag atggaggagg gaatgggtcc cttcgacgcc gccattgagg
     3361 ccgcgggtct tcgcttccgc ccgattctga tgacgtcctt tgcattcgtg ctcggcgtcg
     3421 tgccgctcat catcgcgacg ggcgcaggcg cgggggcccg ccactccatg ggtacgggcg
     3481 tcttcggcgg aatgctcgcc gccaccttca tctcgaccat cttcgtgccg gtgttcttca
     3541 cctggttcgc gaggaagtcg tcgaagaagc gctgatcagg aaaggctcca cgcaaatcgg
     3601 ccgcccctta cggggcggcc gatttttctt tgcggttctg cagcggttcc actgaacgtc
     3661 agccgagcag catcttgtcg gaaacgccgt cgccggcctt ctcgaagaac tgcaggaggt
     3721 ccacggcagt gagcttcgcc ttctcctcgc ccctgacgtc aaggatcgga cggccgtcgt
     3781 gcatcatgat gaggcggttg ccgtagcgga gagcgtcacg catgttgtgc gtgatcatga
     3841 gcgtcgtgag ctgctgttcg gtcacgagct tttcggtaag ttcaagcacc ttggcggcgg
     3901 tcttcgggtc gagtgcggcc gtatgttcgt caagcagcag gagcttggga cgcacgagcg
     3961 tggccatcag cagcgtgagc gcctgacgct gaccgcccga gagcaggccc acatgggccg
     4021 tcatgcggtt ctcaaggccg aggccgagca tcttgacgag ctccgtgaag taggccgtcc
     4081 tgtccgcacg gtcgctccag cgcagcgtct ggctgcggcc gcggcggtcg gcgatggcaa
     4141 ggttttcgga aatcatcatg cctgcggccg tgccgcgcat cggatcctga aagacgcggc
     4201 cgatgaaggc ggcgcgcttg tactcgggca ttctggagac atcgacgccg tcgatgagaa
     4261 tggagccgga gtcgatcggg aacgcgcccg cgatggcatt gagcgtcgtg gacttgcccg
     4321 cgccgttcga accgatcacg gtgcagaagt caccgtcctc gagcgtgaac gagacgcctc
     4381 gcagcgcctt cttttcattg gccgtgccgg cgaagaaagt cttggtgacg tttttgagtt
     4441 cgagcatttt ttctttcctt cgtgttcagt tcacaagtca ggcgcgggcg cgcaggccgg
     4501 ccatcttttc cttgatgagc gggagtgcca gcgcaatggc gacgagcacg gcggtaaaga
     4561 gcttgaggtc gttgggcggc atgcccattt caagcacgaa tgcaatgacg agtcggtaga
     4621 tgatggagcc gagaatcacg gcggcgaggc tcgtcttgaa cgtcttaggg gagaagagca
     4681 cctcgccgat gatgacggag gcaaggccga tcacgatcgt gcccacgccc atgccgacgt
     4741 cggcgaagcc gttggcctga gcgacggtgg cgcccgagag agcgacaagg ccgttcgaga
     4801 tgacgaggcc gagaacgacc atcgtgttgg tgttgatgcc ttgggcgcgg gccatctgcg
     4861 gattgaagcc tgtggcgcgc acggcggtgc cgagctccgt gccgaagaac cagtagatca
     4921 ccacagcgat ggcgactgcg aagacgaggc cgacgacgag gccgctcatg gcgacggaaa
     4981 ggccgaggct gttttcaaga atcgtgaaga ccgtctcatt ctgcagaaga ccgacgttgg
     5041 acttgcccat cacgtgcagg ttcacggaat agaggccgat catcgtcaga atgccggcga
     5101 gcagcgcggg aatgcggagc ttcgtcgtca gcaggccggt gacgccgccc gcgagcgaac
     5161 ccgcgacgag cgccagaaca aaggcggcgg gcagaccgag tcccgccgtg atggcggtgg
     5221 cggccacggc cgcgcccaat gggaacgagc cttcgaccga caggtcggcg atgtcgagaa
     5281 ttcggaaggt caggaagacg ccgagcgcga gaacggacca ctgaagtccc tggccaacgg
     5341 tcgagagaat gagatcaagc atgaaatttc cttttctagg tacgctcggc ggttcgggtt
     5401 gccgattgtt ttttaagtag gagcgccggc ggaatctgtt cctgccggcc tattcaaaaa
     5461 aatgcggagc tccgcagacg ccgtaccggc atccccgaca agctccgctc taggcgcggc
     5521 ctccttcaga ggctactgaa agagcactgc gcccttcttg agttcgtccg gaatcgtgat
     5581 gccgatcgcg gctgccgtct tcatgttgaa gaccggcgtg ccgttggcca cgtgctggac
     5641 gggcatctcg gccggcttct tcttgccgtc gagaatgtcg gcgcccatct ggcccgtcat
     5701 gcggccaatt tcgtagtagt cgaccgagat cgaaccgaga cagcccgctt cggtcatgcc
     5761 gcgctcaccc gcaatgacgg ggatcttgga ggggttggcg accttggcga gggcgggaat
     5821 ggcgctggca agcacgttgt ccgtcggcag ccacatgccc tgcaccttgc cgttgaggct
     5881 ggcagcggcc tgctggatgt cgttgacgtt ggagaccgtg gcctcgagca cctgcacgcc
     5941 gcgcttttcg gcggccttct tgaagatgtc gacctggtac ttggagttga tttcgctgga
     6001 gttgtagatc gtgccgacgg ccttggcgtc ggggacgagc ttgagaagaa gttcgagctg
     6061 ggcctcgatc ggaccgaggt cggagacgcc cgtgacgttg ccgccgggct gttcgtcgga
     6121 cttgacaagc ttggcagcga cgaagtcggt gatggcggtc gcaacgatcg gcgtgccctt
     6181 ggcggtcgtg gccacggcct gggcggcggg cgtcgcgacg gcgaagatca gcttgtcctt
     6241 gttggagacg aagcgggtgg cgatgttgtg cagattcgac tggtcgccct gggcgttctg
     6301 gaagtcgatc ttgatgttga cgccgtcctt gtagccgcgt tcggcaagcg cgtccttgac
     6361 acccttggtg caggcgtcga gggcggagtg ttcaacgagc tggacgatgc ccacgggcac
     6421 gagttcggac ttggcgggct ccttgtcgcc gcagcccgcg agtgtcagcg cggcagcgac
     6481 ggaaagcgcg atggcgctgc ggcgcgtaat cttggaaatc tgcatggggt cacccctgag
     6541 taatgaaagt cgaatgccag ggcttgtagc ccgaggatgt taatttcgat tatacgaacc
     6601 gaaatttgcc gtcttccgcc ctctaattcc ttttacttaa agcaaatccc ctaggcacat
     6661 gaagctcgct caaaagatgc gcaaatcttt tttcccaccc tgcccgacgc ttcgaagtcg
     6721 cataaaaaaa ggttcatctc ggaagtccgt ggaacgcggc cttgactttt aagaaaaagt
     6781 acagatcgtc tcggaagcca taagctcgtc gcttaatttg cttg
//
LOCUS       DBMDTI010000096         6814 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2898_length_6814_cov_2.558767, whole genome shotgun sequence.
ACCESSION   DBMDTI010000096 DBMDTI010000000
VERSION     DBMDTI010000096.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2898_length_6814_cov_2.558767"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..3286)
                     /locus_tag="ACZPB6_05845"
     CDS             complement(<1..3286)
                     /locus_tag="ACZPB6_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HIR1184026.1"
                     /translation="MTEMTAPARVREIPYNYTSLSDREIVIRLLGEKAWDTIESLRGS
                     RRTGRSARMLFEVLGDIWAVERNPYLVDDLIVHPKRRKLLVREMSRRLEAVRQRAEGN
                     ADVLRLVSEASAAVCCFDNSFPKLIERRAEVKRRLSAITDPDNIRFDGFARVTHVTDA
                     TDWRIEYPFVVLTPDREEEIAPLVRALFELKLTIIPRSGGTGYTGGAVPLHPDTAVIN
                     MEKFDRHSGPVMRRLPGVEGEHPVIECGAGCVTRRIEEAAAAAGLVFAVDPASADACC
                     IGGNVAINAGGKKAALWGTTIDNLAWWKMVDVNGRMIRVERVNHNFGRIHEVETAEFD
                     IHELSDEGAVVRTERLSIPGRTFRKAGLGKDVTNKLLNGLPAIQKEGTDGIIVSAAFV
                     LHRMPDFGRTVCLEFYGTVAESTPAIVEVRDFVRGHPTVELAGLEHIDWRYVRAVGYR
                     AKLEGRGMPKMVLLADIVSDNEADLDAVAERCCEMARARGGAGFIARTSADRKAYWKE
                     RSRTAAIAKHTNAFKLNEDVVIPLERLGEYSDEIERINIEFSFKNKIRIAGAFIERLA
                     DTALDERPGITFARDLAARVRTRWEEMRAAMDGDAAPYLAEGVEPREGETVFLAMRDG
                     RLRISVKREILEILAEALPADVMAELHALHKKHVRSRLFVALHMHAGDGNVHTNIPVN
                     SDDYGMMQDAEHTLGRIMSCAVKLDGVITGEHGIGLTKFEFVTDEELRPFREWKNRID
                     PEGRFNKGKLMPGGGLERAYTPSFELFRAESLILENSGLGAISQSVKSCLRCGKCKPV
                     CTTHVPRANMLYSPRNKILALGLMTEAFLYESQTRRGLSLKHFNELVDLADHCTICHR
                     CVKPCPVKIDFGKVTTAVKAFLNRSGHRDLNPAALAGGALVDAVNPIAVRALHAGAVR
                     AGFSLQRLGNELAEKYLAADLARIREKPANTTGRPSLKDQVIHLVSTPLPGRMPRQTM
                     RAALGIEDEITIPILRRPDMPADAEAVFYFPGCGSERLFGDVGLAVEAMLMHAGVQTV
                     LPPGYRCCGHPQLAYGETAKAEKMMTENRVLFHRMANTLNYLDIKTVVVSCGTCIKAL
                     DEYEFDR"
     gene            3680..3931
                     /locus_tag="ACZPB6_05850"
     CDS             3680..3931
                     /locus_tag="ACZPB6_05850"
                     /inference="COORDINATES: protein motif:HMM:NF015956.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1184027.1"
                     /translation="MSDTFTLDKAAIGDELEVVGVHAEGAPEDWEIWLEEIGFTAGEP
                     CVVLQKAALGGDPIAVRIGVSTFALRLVEARCIEVRRRD"
     gene            3931..5778
                     /gene="feoB"
                     /locus_tag="ACZPB6_05855"
     CDS             3931..5778
                     /gene="feoB"
                     /locus_tag="ACZPB6_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HIR1184028.1"
                     /translation="MAVSTVDIYPSGPLIAMLGNPNAGKTSLFNILTGSKQKVANYAG
                     VTVERVEGQLIGAPEKIRVLDLPGTYSFHPRSPDEKVTVDVLKGVARGERRPDLVVCV
                     LDATNLRRGLKLVMAAQRLKLPAVVAVNMMDLARRRGIELKAEDLSRELGVPVVETIA
                     VRKQGIDRLTELLSDPSAWSSWVPEATEEDDLARLNRILRKLGLDKIFPDEFSDRIDR
                     VVLNPWLGVTILMIILFFVFQAVFAWSAYPMEWIEEGFNMLGELCAEHLPDGFVKDLL
                     VDAVIGGVGGVVVFLPQILILFFFILLMEESGYLPRAAFLLDRTMGSVGLSGRSFLPL
                     LSGYACAVPAIMAARIVGNQRDRWATIAIAPLLTCGARLPVYAVMIAAFVPDVSVWGV
                     GLQGLVLFALYCLGMVGALVVAWILKHTTGKGENRTLMLELPSYHLPRIDALAHGLWQ
                     RACIYLQKVGTIILALTVFLWVLTTFPGAPAGATDPAINYSFAGMLGRALEPLFAPIG
                     FNWQMCISLVPAMGAREVAVSVLGTLYATASDAEDALVPILQHSWTIPMGLSFLAFFV
                     FSPSCFATLAAIKREMGTWKAPAVLWVAYTLLAYAFAFVVYRTALWVCG"
     gene            6540..>6814
                     /locus_tag="ACZPB6_05860"
     CDS             6540..>6814
                     /locus_tag="ACZPB6_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184029.1"
                     /translation="MSEVGNEDAGGVLARLMRGANLRRSVEVLKAPDESLAATVSALA
                     NTVGGWILIGPDPEDGWRSAAGFDAEAAVEALRGALGAVESEVRAEAG"
BASE COUNT         1088 a         2058 c         2414 g         1254 t
ORIGIN      
        1 tgcggtcgaa ctcgtactcg tcgagcgcct tgatgcaggt gccgcagctc acgacgacgg
       61 tcttgatgtc gaggtagttg agcgtgttgg ccatgcggtg gaagagcacg cggttctccg
      121 tcatcatctt ctcggccttg gcggtctcgc cgtaggcgag ctgcgggtgg ccgcagcagc
      181 ggtagcccgg cgggagcacg gtctggacgc ccgcgtgcat gagcatcgcc tcgacggcga
      241 ggcccacgtc gccgaagagg cgttcggagc cgcagcccgg gaagtagaag acggcctcgg
      301 cgtcggcggg catgtcggga cgccgaagaa tcgggatcgt gatctcgtcc tcgatgccga
      361 gcgcggcgcg catggtctgt ctgggcatgc ggcccggaag cggcgtcgag acgagatgga
      421 tcacctggtc cttgaggctc ggacggcccg tggtgttggc gggcttttca cgaatgcgcg
      481 caaggtccgc ggcgaggtac ttctcggcga gctcgttgcc gaggcgctgc agcgagaagc
      541 ccgcgcgaac ggcgcccgcg tggagcgcac gcacggcgat cgggttgacg gcgtcgacga
      601 gcgcgccgcc cgcgagcgcg gcgggattga gatccctgtg gcccgagcgg ttgaggaagg
      661 ccttgacggc agtggtcact ttgccgaagt cgatcttgac ggggcagggc ttgacgcagc
      721 ggtggcagat cgtgcagtgg tcggccagat cgacgagctc gttgaagtgc ttgagcgaaa
      781 ggccgcggcg ggtctgcgac tcatagagga aggcctccgt catcagcccg agcgcgagga
      841 tcttgttgcg cggcgaatag agcatgttgg cgcgcgggac gtgcgtcgtg cagacgggct
      901 tgcacttgcc gcagcgcagg cagctcttga cggactgcga gatggcgccg aggcccgaat
      961 tttcgagaat gagggactcc gcgcggaaga gctcgaacga gggcgtgtag gcgcgctcga
     1021 ggccgccgcc cggcatgagc ttgcccttgt tgaagcggcc ctcggggtcg atcctgttct
     1081 tccattcgcg gaagggacga agctcctcgt cggtgacgaa ctcgaacttc gtgaggccga
     1141 tgccgtgctc gcccgtgatg acgccgtcga gcttcacggc gcagctcatg atgcgcccga
     1201 gcgtgtgctc ggcgtcctgc atcatgccgt agtcgtcgga gttgacgggg atgttcgtgt
     1261 ggacgttgcc gtcgcccgcg tgcatgtgga gcgcgacgaa gaggcggctc ctcacgtgct
     1321 tcttgtggag cgcgtgaagc tcggccatca cgtcggcggg cagcgcctcg gcgaggatct
     1381 cgaggatctc gcgcttgacg gagatgcgaa ggcgcccgtc gcgcatggcg agaaacacgg
     1441 tctcgccctc gcggggctcg acgccttcgg cgagataggg cgcggcgtcg ccgtccatgg
     1501 cggcgcgcat ttcctcccag cgggtcctga cgcgggccgc gaggtcgcgc gcgaaggtga
     1561 tgccgggacg ctcgtcgagc gcggtgtcgg cgaggcgctc gatgaaggcg cccgcgatgc
     1621 ggatcttgtt cttgaaggag aactcgatgt tgatgcgctc gatctcgtcg gagtattcgc
     1681 cgagacgctc gagcggaatg acgacgtcct cgttgagctt gaaggcgttg gtgtgcttgg
     1741 cgatggctgc cgtgcgggag cgctccttcc agtaggcctt gcgatcggcg gacgtgcggg
     1801 cgatgaagcc tgcgccgccg cgggcgcgcg ccatttcaca gcagcgttcg gccacggcgt
     1861 cgaggtcggc ttcgttgtcg gagacgatgt cggcgagaag caccatcttg ggcatgccgc
     1921 ggccctcgag cttggcacgg tagccgacgg cgcgcacgta gcgccagtcg atgtgctcga
     1981 gacccgcaag ctcgaccgtc ggatggccgc gcacgaagtc gcgcacctcg acgatggcgg
     2041 gtgtggattc ggccacggtg ccgtagaact cgaggcagac cgtgcggccg aagtcgggca
     2101 tccggtgcag cacgaaggcg gcgctcacga tgatgccgtc cgtgccttcc ttctgtatgg
     2161 cgggcaggcc gttgagaagc ttgttggtga cgtccttgcc gaggcccgcc ttgcggaagg
     2221 tgcggcccgg aatcgagagg cgctctgtcc tgacgaccgc gccctcgtcg gagagctcgt
     2281 ggatgtcgaa ctcggcggtc tcgacctcgt ggatgcggcc gaagttgtgg ttgacgcgct
     2341 cgacgcggat catgcggccg ttgacgtcga ccatcttcca ccaggcgagg ttgtcgatcg
     2401 tcgtgcccca gagggcggct ttcttgccgc ccgcgttgat ggcgacgttg ccgccgatgc
     2461 agcaggcgtc ggccgaggcg gggtcgaccg cgaagacgag gcctgcggcg gccgcggcct
     2521 cctcgatgcg gcgggtgacg cagcccgcgc cgcattcaat cacgggatgc tcgccctcga
     2581 cgcccggcag gcggcgcatg acgggaccgc tgtggcggtc gaacttctcc atgttgatca
     2641 cggccgtgtc ggggtgcagc ggaacggcgc cgcccgtgta gcccgtgccg cccgagcgcg
     2701 gaatgatggt gagcttgagc tcgaagagcg cgcgcacgag gggcgcgatc tcctcctcgc
     2761 ggtcgggcgt gaggacgacg aacggatact cgatgcgcca gtcggtggcg tcggtgacgt
     2821 gcgtgacgcg cgcaaagccg tcgaagcgga tgttgtccgg atcggtgatc gcggacagac
     2881 ggcgcttcac ttcggcacgg cgctcgatga gcttcgggaa gctgttgtcg aagcagcaca
     2941 cggcggcgct cgcttcggag acgaggcgaa gcacgtcggc attgccctca gcgcgctggc
     3001 gaacggcctc gaggcggcgg ctcatctcgc gcacgagcag tttgcggcgc ttcgggtgca
     3061 cgatcaggtc gtcgaccaga tagggattgc gctcgaccgc ccagatgtcg ccgagcactt
     3121 cgaagagcat gcgggcggaa cggccggtgc ggcgagagcc gcgaagagac tcaatcgtgt
     3181 cccaggcctt ttcgcccagg agacggatca caatctcgcg gtccgagagc gacgtgtagt
     3241 tatagggaat ctcgcgaacg cgcgcgggag cggtcatctc ggtcatcagg gaggaactcc
     3301 gttggtgagg cgggttcggc gtcaggcgcg aaccgcattg aatgacaggg gattctttca
     3361 ttctaaccca ttgattttca agggttatac atcagattga ctagggtgaa atgggtgcaa
     3421 ataccccctt gggagtattc gtcgtaaggg aaaactgtca tttttgggtg acagcgcgcc
     3481 tcttcgggag gcggaggcgg tccaagcggg cgaggaattt tttcagggaa tcgagccgac
     3541 ggctgcgcaa acgggtaaaa tgcgagtcac ctaaaagaga atcattttcc ttctcagccc
     3601 tcactcacgg ggctggaccc gggcttccga acccctgagg cggcccgagg cacgaaggat
     3661 cagcccccca tatataaaaa tgtccgacac attcactctg gacaaggccg ccatcggaga
     3721 cgagctcgag gtggtcggcg tccatgccga aggcgctccc gaagactggg agatctggct
     3781 tgaggaaatc ggcttcaccg cgggcgaacc gtgcgtggtg ttgcagaagg ccgctctggg
     3841 cggcgatccg atcgcggtcc gcatcggcgt ctcgacgttt gcgctgaggt tggtcgaggc
     3901 ccgctgcatc gaggtgagga ggcgcgactg atggccgtct ccaccgttga catctatccg
     3961 tccgggccgc tcatcgcgat gctcggcaat ccgaacgccg gcaagacgtc gctcttcaac
     4021 attctgacgg gttcgaagca gaaggtcgcc aactatgcgg gcgtgaccgt cgagcgcgtc
     4081 gagggccaac tcatcggcgc gcccgagaag atccgcgtgc tcgaccttcc gggcacctat
     4141 tcctttcatc cgcgctcgcc cgacgagaag gtgacggtcg acgtcctcaa gggcgtcgcg
     4201 cgcggcgagc gcaggcccga cctcgtcgtc tgcgtgcttg acgcgacgaa cctgaggcgc
     4261 ggcctcaagc tcgtcatggc cgcgcagcgc ctgaagctcc ccgccgtggt ggcggttaac
     4321 atgatggacc tcgcgcgccg ccgcgggatc gagctcaagg ccgaggacct ctcgcgcgag
     4381 ctcggcgtgc ccgtggtcga gacgatcgcc gtgcgcaagc agggcatcga caggctcacc
     4441 gagcttctct ccgacccatc cgcctggtcg tcttgggtgc ccgaggcgac cgaggaggac
     4501 gaccttgcgc gcctcaacag gatcctgcgc aagctcgggc ttgacaagat cttccccgac
     4561 gaattctccg accgcatcga ccgcgtcgtg ctcaatcctt ggctcggcgt gacgatcctg
     4621 atgatcatcc tcttcttcgt cttccaggcg gtgttcgcct ggtccgccta tccgatggag
     4681 tggatcgagg agggcttcaa catgctcgga gagctgtgcg ccgagcacct gcccgacggg
     4741 ttcgtgaagg acctgctcgt cgacgccgtc atcggcggcg tgggcggcgt ggtggtgttc
     4801 ctgccgcaga tcctgatcct cttcttcttc atcctgctga tggaggagtc gggctacctg
     4861 ccgcgagcgg cgttcctgct tgaccgcacg atggggtcgg tgggcctttc gggccgctcc
     4921 ttcctgccgc ttctctcggg ctacgcctgc gcggtgcccg ccatcatggc ggctcgcatc
     4981 gtcggcaacc agcgcgaccg ctgggcgacg atcgccattg cgccgctact cacctgcggc
     5041 gcgaggctgc ccgtctacgc cgtgatgatc gcggccttcg tgcccgacgt ctccgtctgg
     5101 ggcgtgggcc tgcagggcct cgtgctcttc gcgctctact gtctcggcat ggtgggcgcg
     5161 ctcgtcgtcg cctggatcct caagcacacg acgggcaagg gcgagaaccg cacgctgatg
     5221 ctcgagctgc cgagctacca cctgccgcgc atcgacgcgc tcgcgcacgg cctctggcag
     5281 cgcgcctgca tctatctgca gaaggtgggc acgatcattc tggcgctcac ggtcttcctc
     5341 tgggtgctga cgacctttcc gggcgcgccc gccggcgcga ccgatcccgc gatcaactac
     5401 agcttcgcgg gcatgctcgg ccgcgcgttg gagcccctct ttgcgccgat cggcttcaac
     5461 tggcagatgt gcatctcgct cgttcccgcc atgggcgcgc gcgaggtggc cgtctcggtg
     5521 ctcggcacgc tctacgcgac cgcgagcgac gcggaggacg ccctcgtgcc gatcctgcag
     5581 cacagctgga cgatcccgat gggcctctcc ttcctcgcct tcttcgtttt cagcccgtcg
     5641 tgcttcgcga cgctcgccgc catcaagcgc gagatgggca cctggaaggc gccggctgtc
     5701 ctgtgggtgg cctacacgct cctcgcttac gccttcgcct tcgtcgtcta ccgcacggcc
     5761 ctctgggtct gcggctaagc ccggaaaggg cgccgtcgag ggacgggcaa gaacaattcg
     5821 ggcgcctgcg gcagatgctg cgggcgcccg aattgttttc aggactgctt cacgatgaag
     5881 cgctcgacga cgtccgcggc tccgagtacg cgggcgcgcc tgaggtatcc gggatgatgg
     5941 aacctcgtgc agcccatacc cgaaggatcg gttcgaggat cagaacgtcc tttgcggcat
     6001 ggtgcatttt gaaggccact tcaaggggac gtacggtctt cgcgccaaag agggcctcaa
     6061 aatgcgccga ggccgctcga tgaaaatcag ctcgcaaagt atcttcgacc agcgttcagc
     6121 ccgtccttcg gggcgagacg gcgggctcgc aggctctgaa gacaagcgca tcggaacccg
     6181 tcgagctgaa gctccagttg ttgcatgacg atgcattatc gagtgacgga gggattgcaa
     6241 ctgtaaaggg tggaaaggtg gtttggcgtg gtttacagtc atgagtggaa atgaccggga
     6301 tgcttttccg tgagccggcc ggaggaagag agtcggcaag tgtaaaggta aaatatgtct
     6361 gatctgtagt ttaaagtatg cgttactgtc agcgggcggg gttgacagtt ctggtaccgc
     6421 atgtttacag tttgagctgt cgcggggcca tttccggttg cgcgacgggg ctgcgggagc
     6481 ggacggagtg caccccggcg gaagggttta cagttgattt gtcaagtcgg gaggacggca
     6541 tgtctgaagt cgggaatgag gacgcgggcg gcgtgctggc gcgtctcatg aggggcgcga
     6601 atctgcgcag gagtgtcgag gtgctcaagg cgcccgacga gagtcttgca gcgacggttt
     6661 cggcccttgc gaacacggtg ggcggatgga ttctgatcgg ccccgatcct gaggacggat
     6721 ggcggtctgc ggcggggttc gatgccgagg cggcggtcga ggcccttcgc ggggcgctcg
     6781 gggcggtgga gtctgaggtg cgcgcggagg cggg
//
LOCUS       DBMDTI010000097         6807 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2903_length_6807_cov_3.300030, whole genome shotgun sequence.
ACCESSION   DBMDTI010000097 DBMDTI010000000
VERSION     DBMDTI010000097.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2903_length_6807_cov_3.300030"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            11..101
                     /locus_tag="ACZPB6_05865"
     tRNA            11..101
                     /locus_tag="ACZPB6_05865"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Ser,seq:gga)
     gene            305..395
                     /locus_tag="ACZPB6_05870"
     tRNA            305..395
                     /locus_tag="ACZPB6_05870"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:339..341,aa:Ser,seq:gga)
     gene            512..907
                     /locus_tag="ACZPB6_05875"
     CDS             512..907
                     /locus_tag="ACZPB6_05875"
                     /inference="COORDINATES: protein motif:HMM:NF025386.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HIR1184030.1"
                     /translation="MALFFRKRIKILPGVTLNLSKSGVSATVGRKGASVSVGKRGTYL
                     NAGQPGTGIYSRTRLDKPAGRRRSAGKANAEIEAPEQTDPVTESAAAPEAPARAPGGL
                     ESAVALLKCIALLAFIGLCLAGIWSLLAD"
     gene            1058..2809
                     /locus_tag="ACZPB6_05880"
     CDS             1058..2809
                     /locus_tag="ACZPB6_05880"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106219 - zinc ion sensor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HIR1184031.1"
                     /translation="MKIKTATAAVSAALLSLIAAGQAGAAQIYPVDQARFMTNARFDF
                     KIELDKSVARKDITVQINGADYSKTLSGKEVWMEKEDNVHGSALVVRDVEIKKPGRYE
                     VIVKSPEGEMRANWDIYTTPEKPVAKNVIILLADGLSVGHRTAARILSKGVTNGMYNA
                     PLAMDDMPHMALLGTSSVDTIAADSANTASAYMTGHKSSVNALGVYADRTQASQDDPK
                     QETIAELLRRKTNKAIGVVSDAEIEDATPASVVSHTRRRADKADIVGMFYNVKPEVIL
                     GGGSAYFLPKETAGSKRKDSINYIQKFGDAGYKLVTDRTEMLSAVSGQTPDRLLGLFH
                     TGNMDGWVDRHQWKGNTVKKFPNQPDLTESFDAAVKVLEKNKDGFFLILEAGLVDKYS
                     HPLDWTRSVGDTIAFDKVVERAKAYCAKNPDTLLIVTGDHTHSISVAGTVDDNKPGKD
                     MREKVGVYADAGYPNYEDKDKDGFPDNFQPSKRLAVFFGAHPDYYETYRSYPDATFNP
                     AVKNEKGEYVADARYKDVEGGHFVEGNLPRNESQGVHTVDDLVVTAQGPNAEQIRGFM
                     NSTEVFRVMAEALALGK"
     gene            2910..3350
                     /locus_tag="ACZPB6_05885"
     CDS             2910..3350
                     /locus_tag="ACZPB6_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184032.1"
                     /translation="MQRRQFVKCLLGTPLLVAVPAFAAVPELRFEEMYAGNSVLGLQF
                     SEKLKSLSGKRIRIRGFMAPPLKAEAAFLILTKEPVSLCPFCNSDQDWPDSIILVRLA
                     SEQAFVQNNRLIEVTGRLETGSETDPETGFVSLVRLADAKYEVI"
     gene            3350..4027
                     /locus_tag="ACZPB6_05890"
     CDS             3350..4027
                     /locus_tag="ACZPB6_05890"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1184033.1"
                     /translation="MTDTLKLEGVGLDVRDGSSLRTLLEIDEFCLEPGEMIAVRGASG
                     AGKTTFLKLVSGILLPTRGRVLYGDTEVSALSEPRRDRWRGRHVGFLFQDFRLFDGLT
                     ALENVLLPFTFCSRTVSNTQRRDATDLLKLHGVNPDTRSELLSRGEMQRTALVRVLMQ
                     SPSIILADEPTASLDANRVGHAVDALISAAGSLGASLVLVSHDERVLGHFERHLTLAD
                     GRLTPRI"
     gene            4031..5323
                     /locus_tag="ACZPB6_05895"
     CDS             4031..5323
                     /locus_tag="ACZPB6_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR1184034.1"
                     /translation="MNVLMLLRSELRRSAAVLAGIAAVLALSLSISIATGMTDRMLRH
                     ASAQAADRFDLLIGAKASPSSLLLGAVFLRDEPLPLVPLSVMKDLDERHGVKWAAPVA
                     FGDRAGDSPIVGTTTSLVTFGGTVRPAEGRLFKAPFEAVVGASAPYRIGDEIVPMHGR
                     TPGAGHAHDHGRLKVVGRMPESGTPWDRAVMIPIEAVWATHSMTVHDELERAHGYDHE
                     EEDGTEHEYAHEEGHGRLLGVFSEHDFETLPGVSAVVVKPASFADAYRLRQQQSQRTL
                     SGPDRTSVNLMGVFSGEVLVSLHSLLGGASEAVTITARLTLLTALAATLIMGVLLGEL
                     RRPALEQLRVLGAPQRYIMSLVWCIVMTVVAAGTAGGLVLGFIGAEAAAHLLAAETGV
                     AMSPAMGSPELLAAAATLLAGSFCALIPAWLAGRRPIR"
     gene            5761..6726
                     /locus_tag="ACZPB6_05900"
     CDS             5761..6726
                     /locus_tag="ACZPB6_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HIR1184035.1"
                     /translation="MSPVDMARESDVVQTSYEIIRAAAGRLRDPELRKAILSIIENPA
                     PTIASADQSAVLAALKKEGLIAAGRTSVFPKFSDTTRSPQPTWSAPGSGYGSHHAYPG
                     GLCTHVALNVVSAESLVAAYDNIDGLKLDFDHAVGGEILHDLHKPWVFQWEADNACRK
                     EEALAGTGEHHVLSIAESIKRGLPAEFVVAQACAHEHPGSASGEAQVVGWLRAAAIIA
                     GVDPVKAGLIAADGKTLPLPRRIEGWVVHLADHDWVISVPACQWVVKALRNLAEKKWG
                     VRDEKTFNALRNYVLCNLTAMRLYGILSAQGEEAFAADVARVVKA"
BASE COUNT         1264 a         2497 c         1927 g         1119 t
ORIGIN      
        1 gagcacctat ggaagggtgg cagagtggtt gaatgtaccg ccctggaaag gcggcatacg
       61 gaaattccgt atcgagggtt cgaatccctc cccttccgcc aagattcaga aaggcaggaa
      121 gcttcggctt cctgcctttt ttcatgcgtg cggcggcgtc ccggcacatc ccgcatgccg
      181 tctgcagttt ttttcaaacg tttcttgact ttttcaaaaa actcaggcat acttcgtctc
      241 ctcagcaaat tcgacgtgtc gacaaagcca atcaccacgg ccggcatgtt cgctgagcac
      301 ctatggaagg gtggcagagt ggttgaatgt accgccctgg aaaggcggca tacggaaatt
      361 ccgtatcgag ggttcgaatc cctccccttc cgccagaatt cgaaaggcag aaagcttcgg
      421 ctttctgcct tttttgctat tgggctccgg ccgcgcacgc ctgcgccgtc cgagcctgaa
      481 tcctcccggc aaacaagctt cccgaacagc catggcactc tttttcagaa agcgcatcaa
      541 gattcttccc ggcgtcacgc tcaacctctc caagtcgggc gtcagcgcca cggtcggccg
      601 caagggcgcg agcgtctcgg tcggcaagcg cggcacctac ctcaacgctg ggcagccggg
      661 cacgggcatc tacagccgca cccgcctcga caagcctgca ggccgacgcc gcagcgcagg
      721 aaaggcgaac gcggagatcg aagcgcccga gcagacggat cccgtgacgg aaagcgccgc
      781 cgcgcccgag gcgcccgccc gtgcgccagg cggtcttgag tccgccgtcg cgcttctcaa
      841 gtgcatcgcg ctcctcgcct tcatcggcct ctgccttgcg ggcatctgga gccttctcgc
      901 cgactgagtc ccgtccgaca cggttccgtc atcatttcct cctcgcggtc atcatcaatt
      961 cacgaagccg acacgggatc gtcaaatcga attcctagac tttgctccat ccgaaggccc
     1021 ggcgccttcg gaccattgtt tgttgaagga attcattgtg aaaatcaaga ccgcgaccgc
     1081 tgccgtctcc gcagcgcttc tctccctcat tgccgctggc caggccggcg ccgcccagat
     1141 ctatcccgtc gatcaggccc gcttcatgac gaacgcccgc ttcgacttca agatcgagct
     1201 tgacaagagc gtcgcccgca aggacatcac cgttcagatc aacggtgccg actactccaa
     1261 gacgctctcc ggcaaggaag tctggatgga gaaggaggac aacgtccacg gttccgccct
     1321 cgtcgtccgc gacgtcgaga tcaagaagcc cggccgctac gaagtcatcg tcaagagccc
     1381 cgaaggcgaa atgcgcgcca actgggacat ctacacgacg ccagaaaagc cggttgccaa
     1441 gaacgtcatc attcttcttg ccgacggcct ctccgtcggt caccgcaccg ccgcccgcat
     1501 cctctccaag ggcgtgacga acggcatgta caacgctccg ctcgccatgg acgacatgcc
     1561 gcacatggct cttctcggca cgagctccgt cgacacgatc gccgccgaca gcgccaacac
     1621 cgcctccgcc tacatgacgg gccacaagtc gagcgtcaac gccctcggcg tctacgccga
     1681 ccgcacccag gccagccagg acgatccgaa gcaggagacg atcgccgagc ttctgcgccg
     1741 caagaccaac aaggccatcg gcgtcgtgag cgacgccgag atcgaggacg ccacgcccgc
     1801 ctccgtcgtc tcccacaccc gccgccgcgc cgacaaggcc gacatcgtcg ggatgttcta
     1861 caacgtcaag cccgaggtca tcctcggcgg cggctccgcc tacttcctcc cgaaggaaac
     1921 cgccggctcc aagcgcaagg acagcatcaa ctacatccag aagttcggcg acgccggcta
     1981 caagctcgtc acggaccgca cggaaatgct cagcgccgtc tccggccaga cgcccgaccg
     2041 cctgctcggc ctctttcaca ccggcaacat ggacggctgg gtcgaccgcc accagtggaa
     2101 gggcaacacc gtcaagaagt tcccgaacca gcctgatctg accgaatcct tcgacgccgc
     2161 cgtcaaagtg ctcgagaaga acaaggacgg cttcttcctg atactcgagg cgggcctcgt
     2221 ggacaagtac tcccacccgc tcgactggac ccgctccgtg ggcgacacga tcgccttcga
     2281 caaggtggtc gagcgcgcca aggcctactg cgccaagaac cccgacacgc tgctcatcgt
     2341 gacgggcgac cacacgcact cgatctccgt cgccggcacg gtggacgaca acaagccggg
     2401 caaggacatg cgcgaaaagg tgggcgtcta tgccgacgct ggctacccga actacgagga
     2461 caaggacaag gacggcttcc ccgacaactt ccagccctcc aagcgtcttg ccgtcttctt
     2521 cggtgcccac cccgactact acgaaacgta ccgctcctat cctgacgcca ccttcaaccc
     2581 ggccgtcaag aacgaaaagg gcgaatacgt cgccgacgcc aggtacaagg acgtcgaggg
     2641 cggccacttc gtcgagggca acctcccgcg aaacgaaagc cagggcgtgc acacggtcga
     2701 cgatctggtc gtgaccgccc agggtccgaa cgccgaacag atccgcggct tcatgaactc
     2761 cacggaagtc ttccgcgtga tggccgaagc gcttgccctc ggcaagtaac ggtcacaagg
     2821 cccctcgcag ggccccgcgc cggaggcgcg gctcccacgg gagtccgcct ccggcctttt
     2881 caattttcag aaacgagacg ccacccatca tgcagcgccg ccaattcgtc aaatgccttc
     2941 ttgggacccc gctccttgtg gccgtccctg cattcgccgc cgtcccagaa ctcaggtttg
     3001 aggagatgta cgcgggcaac tccgtcctcg gccttcagtt ctccgaaaag ctcaagtcgc
     3061 tctcgggcaa gcggatccgc atccgcggct tcatggctcc gccgctcaag gccgaagccg
     3121 ccttcctcat cctcacgaag gagcccgtga gcctgtgccc cttctgcaac tccgatcagg
     3181 actggcccga cagcatcatc ctcgtgaggc tcgcgagcga gcaggccttc gtgcagaaca
     3241 accgcctcat cgaggtgacg ggacggctcg aaacgggcag cgagaccgat cccgaaacgg
     3301 gattcgtgag cctcgtgcgg ctcgccgacg caaagtacga ggtgatctga tgacggacac
     3361 gctcaagctt gaaggcgtcg gactcgacgt tcgcgacggc tcctccctgc gcacgctcct
     3421 cgagatcgac gagttctgcc tcgaacccgg tgaaatgatc gccgtgcgcg gcgcaagcgg
     3481 cgcgggcaag accaccttcc tcaagctcgt ctcgggcatt cttctcccga cgaggggccg
     3541 cgtgctctac ggcgacaccg aggtctccgc actctccgaa cccaggcgcg accgctggcg
     3601 cggccggcac gtcggcttcc tctttcagga cttccgcctc ttcgacggcc tcacggccct
     3661 cgaaaacgtc ctgctcccct tcacgttctg ctcgcgcacc gtatccaaca cccagcgtcg
     3721 cgacgcgacg gacctcctca agttgcacgg agtcaatccc gacacgcgct ccgaactcct
     3781 ctcgagaggc gagatgcagc gcacggcgct cgtgcgcgtg ctgatgcagt ctccctcgat
     3841 catcctcgcg gacgaaccca ccgcaagcct cgacgccaac cgtgtaggtc atgcggtcga
     3901 cgcactcatc tccgccgcag gctcgctcgg cgcctccctc gtgctcgtaa gccacgacga
     3961 gcgcgtgctc ggccacttcg agcgccacct cacgctcgcc gacggccgac tcaccccaag
     4021 gatctgatcc atgaacgtcc tcatgcttct taggagcgag ctgcgcagaa gcgccgccgt
     4081 gctcgcaggc atcgccgccg tcctcgcact ctcgctctcg atctcgattg cgaccggcat
     4141 gaccgacagg atgctgaggc acgcctcggc ccaggccgcc gaccggttcg atcttctcat
     4201 cggagccaag gccagcccct cgtcccttct gctcggcgcc gtcttcctgc gtgacgaacc
     4261 ccttcccctc gtgccactct ccgtcatgaa ggaccttgac gaaaggcacg gcgtcaagtg
     4321 ggcagccccc gtcgcattcg gcgacagggc gggagactct cccatcgtcg gcacgacaac
     4381 gtcgctcgtc accttcggcg gcacggtcag gcccgccgag ggacgcctct tcaaggcgcc
     4441 cttcgaggcc gtcgtcggcg cctcggcccc ctaccgcatc ggcgacgaaa tcgtacccat
     4501 gcacggcagg acgcccggcg caggacacgc gcacgaccac ggacgcctca aggtcgtggg
     4561 caggatgccc gaaagcggca cgccctggga ccgcgccgtc atgattccga tcgaggccgt
     4621 ctgggccacg cactcgatga cggtgcacga cgaactcgag cgcgcccacg gttacgacca
     4681 cgaagaagaa gacggaacgg agcatgaata tgcgcatgaa gaagggcacg gccgactgct
     4741 cggcgtcttc tccgaacacg acttcgagac gcttcccggc gtctccgccg tcgtcgtcaa
     4801 gcccgcctcg ttcgccgatg cctacaggct gcgccagcag cagtcgcagc gcacgctctc
     4861 cggccccgac cgcacgtcgg tcaacctgat gggcgtcttc tctggcgagg tgctcgtctc
     4921 gctccactcg cttttgggcg gcgcgagcga ggccgtgacg atcacggccc gcctcacgct
     4981 tctcacggcg ctcgccgcca cgctcatcat gggcgtgctg ctcggtgagc tccgcaggcc
     5041 cgccctcgag cagctccgcg tactcggcgc cccgcagcgc tacatcatga gcctcgtctg
     5101 gtgcatcgtc atgacggtcg tcgctgcggg gaccgcgggc ggcctcgtcc tcggcttcat
     5161 cggcgcggag gcggccgcgc atctgcttgc cgccgaaacg ggcgttgcca tgtcgcccgc
     5221 catgggctcg cccgaacttc tcgccgccgc ggccacgctc cttgcgggtt cgttctgcgc
     5281 cctcattccc gcatggctcg cggggaggcg ccccatccgc tgagcgcctc gctccgagcc
     5341 ctctcagagc gcccgtctcc ttcgaagaga cgggcgctcg tcgcatcggg cgtccgaaga
     5401 caatccgtca tcgaaaattc acgcaagcgt cttcaagatg tcaacgcacg gtcacggcgc
     5461 cgtcatgcgg ccctgaaaga atccgtcctg caggctcaag ggaatccggg aaaagctcag
     5521 acccgaagcc ctgaagtccc ggatcgggac cgtcgttgaa cagggcctgc tctgcacagt
     5581 tcatgcagat gttgttttgt gaaaggaaaa tcatgacatc agatctcccg ccgctcgctc
     5641 ttcggcgccg ccgctgcggc ctccgttctc cccgtcgcga gcggcctcgc cgccttcagc
     5701 ccgatcgcag ccgctgccgc cgcacccaaa aaggcccgca ccattgtcga aatcgccgcc
     5761 atgtccccgg tcgacatggc ccgcgagagc gacgtcgtcc agacctcgta cgaaatcatc
     5821 cgcgcggccg ccggccgtct gcgcgatccc gaactcagga aggccatcct ctccatcatc
     5881 gagaacccgg ctcctacgat cgcctccgcc gaccagagcg ccgtgctcgc cgccctcaag
     5941 aaggagggcc tgatcgccgc cggccgcacg tccgtcttcc cgaagttctc cgacaccacc
     6001 cgctcgccgc agcccacctg gagcgcgccg ggctccggtt acggcagcca ccatgcctat
     6061 ccgggcggcc tctgcaccca cgtcgccctc aacgtcgtct ccgccgaatc cctcgtcgcc
     6121 gcctacgaca acatcgacgg cctcaagctc gacttcgacc acgccgtcgg cggtgaaatc
     6181 ctgcacgacc tgcacaagcc ctgggtcttc cagtgggagg ctgacaatgc ctgccgcaag
     6241 gaggaggccc tcgcaggcac gggcgaacac cacgtactca gcatcgccga gtccatcaag
     6301 cgcggcctgc ccgccgaatt cgtcgttgcc caggcctgcg cccacgagca tccgggcagc
     6361 gcctccggcg aagcccaggt cgtcggctgg ctccgcgccg ccgcgatcat cgcgggcgtc
     6421 gaccccgtca aggccggcct catcgccgcc gacggcaaga ccctgccgct gccgcgccgc
     6481 atcgagggct gggtcgtaca cctcgccgac cacgactggg tcatctccgt gcccgcctgc
     6541 cagtgggtcg tcaaggccct tcgaaacctc gctgagaaga agtggggcgt gcgcgatgaa
     6601 aagaccttca acgcgctgcg caactacgtg ctctgcaact tgaccgccat gcgcctctac
     6661 ggcatcctgt cggctcaggg cgaggaggcc ttcgccgccg acgtcgcccg cgtagtcaag
     6721 gcctgatccg cacaagacgc ccgaacgacc gtgcgtttcg cacaagtcga cttatgaaaa
     6781 agccgcactg gacgagaaaa gcccggt
//
LOCUS       DBMDTI010000098         6627 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2985_length_6627_cov_2.014404, whole genome shotgun sequence.
ACCESSION   DBMDTI010000098 DBMDTI010000000
VERSION     DBMDTI010000098.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2985_length_6627_cov_2.014404"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="ACZPB6_05905"
     CDS             complement(<1..372)
                     /locus_tag="ACZPB6_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024418.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HIR1184036.1"
                     /translation="MNKNFKVVFSKARGALMVVNELTSSVQAKGTKTVIAATVASLVA
                     GGAIAKEIPYEQASGNSGYSATTAENLQMEFEADNVLNMTITGKVDTRAYGLLATGAD
                     SVYTNKGTINLNLAADENATQA"
     gene            complement(1468..1992)
                     /locus_tag="ACZPB6_05910"
     CDS             complement(1468..1992)
                     /locus_tag="ACZPB6_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1184037.1"
                     /translation="MSFLKIGTRSRYALRFMIELAEVERAARSDETRRRGYVKLRTVA
                     SSQDISWRYLEQIAPALAEAGMVETGRGPLGGYRLLRNPATLTVAEILAVVESDEAPM
                     KCFDPEDDCSRRAGCAATFLWKGLSDVVDKYLQSVTLQDLLDGRVTVPKLIGARHDEP
                     VRPDANASEIDEKA"
     gene            complement(1989..2813)
                     /gene="queF"
                     /locus_tag="ACZPB6_05915"
     CDS             complement(1989..2813)
                     /gene="queF"
                     /locus_tag="ACZPB6_05915"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435515.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HIR1184038.1"
                     /translation="MSACAPAGTLLGQTTEYVDTYRPDLLTPIPRSLGRDAIGVHDFR
                     GTDVWRLYEITWINERGLPVAAAGEIRVPATSPCIVESKSLKLYIGSLTQTKIASVEE
                     AARLIARDVSACVGAPVEVLIDDLARWEMPVTRVPGIVLEAECGDLECSVYEPDASLL
                     KAASDEVVEETLSSNLLRSRCPVTGQPDHASIVISYRGRRIDRRALLAYIVSFRRHQG
                     FHEQCCEQIHADIMRVLAPEALSVNCCFTRRGGIDISPFRSTVADLPDPVIRTPRQ"
     gene            complement(2951..3223)
                     /locus_tag="ACZPB6_05920"
     CDS             complement(2951..3223)
                     /locus_tag="ACZPB6_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1184039.1"
                     /translation="MNKSEFITALQAKTGLAKADVTKLVNAYGEVVIEAVSKGEDVRL
                     IGFGTFGTSERAARVGRNPHTGQEIQIPAGRVVRFSASANFKEAVK"
     gene            complement(3736..4785)
                     /gene="hypE"
                     /locus_tag="ACZPB6_05925"
     CDS             complement(3736..4785)
                     /gene="hypE"
                     /locus_tag="ACZPB6_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HIR1184040.1"
                     /translation="MTDFYMRPLDMKNGAVDLTHGAGGRATAQLIHDVFGRHFTNVWL
                     DEGHDGALLPPMTRPVAVSCDGHVVRPLFFPGGDIGRLSVAGTVNDVAMCGARPLYLT
                     ASFILEEGFPLKDLDAVVASMAKTCEEAGVAIVTGDTKVVERGHGDGVYIAISGIGEK
                     ISEGTISGRAARPGDAVLISGTMGDHGTAILSFREGMSFGTTVESDCAPLNEMVEAVL
                     SAAPHVHVLRDPTRGGLAATLNEIASQSNVGILLEESTVPVRPEVRSACEFLGLDPLY
                     VANEGKMIVIVPEDEADAALAAMRASPYGENAARIGRVTDDSDVPVGMVEMVTLMGGR
                     RTVDWLAGEQLPRIC"
     gene            complement(4803..5960)
                     /gene="hypD"
                     /locus_tag="ACZPB6_05930"
     CDS             complement(4803..5960)
                     /gene="hypD"
                     /locus_tag="ACZPB6_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HIR1184041.1"
                     /translation="MKYVDEYREGDAARRIAAEIRHEADPKRIYRFMEFCGGHTHVLA
                     RWGLTDILPPNVRMIHGPGCPVCVLPVARIDMAMQLALERNVILCTYADTMRVPAGKG
                     MSLFKAKAQGADIRMIYSPMDALELAKANPEREVVFFAIGFETTTPPTAVVLKAAKAA
                     GVKNFSVFCNHVLTPAAMTHILKMSEERPEVPVLDGLVGPAHVSTIIGSGPYEVFARD
                     NRLPVVVTGFEPLDMLYAILMMVRQVNDGRHEVENEFTRAVTRDGNVAAIRLMDEVFE
                     LRDSFEWRGLGRLPKSALKLRPEWADFDAEKRFAMTERAVTDNKACACGAILRGEKTP
                     EQCPLFGRACNPANPIGACMVSSEGACAAAWSYGRRRAAAPEKAKTSDDQR"
     gene            complement(5957..6193)
                     /locus_tag="ACZPB6_05935"
     CDS             complement(5957..6193)
                     /locus_tag="ACZPB6_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HIR1184042.1"
                     /translation="MCLAIPGQIIRLAGDGEAVCDIGGIEKTVNVSLIERPVPGDWVI
                     VHVGFALNRIDPEEAQRTLAALKAAGLHDEEHAS"
     gene            complement(6195..>6627)
                     /locus_tag="ACZPB6_05940"
     CDS             complement(6195..>6627)
                     /locus_tag="ACZPB6_05940"
                     /inference="COORDINATES: protein motif:HMM:NF046761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Kae1-like domain-containing protein"
                     /protein_id="HIR1184043.1"
                     /translation="TLSLVPLVRRVVDERLSGTSVECLAALVENAVIGALADWIAFHA
                     TKLVEEFGPGLLASDDNENMLVALTGGTMNNAALSKGLVEALTSRGLAGALPLHVPAG
                     DGGLSLGEAWWARSALAAGATEYAPLDASSVLGRVQPQGES"
BASE COUNT         1272 a         1895 c         2228 g         1232 t
ORIGIN      
        1 agcctgcgtg gcattctcgt cggccgcaag attgaggttg atcgtgccct tgttggtgta
       61 aacggaatcg gcacccgtcg caaggagacc gtaggcacgc gtgtcaacct tgcccgtgat
      121 cgtcatgttc agcacattgt cagcctcgaa ctccatctgg agattctcgg ccgtcgtcgc
      181 ggagtagccg gaattgccgg aagcctgttc atatgggatc tctttggcaa tggcgccgcc
      241 cgccacaagc gaagccaccg tagcagcaat caccgtcttc gtgcccttgg cctggacgga
      301 ggacgtcagt tcgttgacga ccatcagggc gccacgcgcc ttgctgaaca caactttgaa
      361 attcttgttc atgttttttc tctacttgat gaaactccgc tgccgaaagt ggcggcggac
      421 acgaagcaga gtaaagaaac atggaatgag gggggggtaa acccttatgc gactcaccag
      481 gatctcaacg aacattgcgc aacggaaggg ggtactgagg aaagcaaagg cccttcggga
      541 acgggacata tttctggaaa aggacgggga tgggatcccg atagggaacg ccatgaagag
      601 agacgcctgc atgaatcctt gcagcaaagg gaaagtgcaa atgcaaacgt tgcacgagtc
      661 tgaagaagga atcactgggg agggtaccgg gattccacat aagacatgtc accgtcttca
      721 gcactcatcg catcaaacgt gaatgaaaaa accgactgta caaacctcca aggcgattaa
      781 agtgtggcga aatcgcaaca atcatctcgc aaagcaaagc accttcaaac acagctcagt
      841 tcgtggcgtt tttatcagtg ttcaggccta acaacgctgc gaaaccaggc tctacgtgac
      901 agcctgccgg caaaacaacg agccttcagg cagagttgtg ggtaaagtca ggcccagaag
      961 gtggtgttga cccgcgccaa cgagcgcgag cacggatgct ggtaagtgat tcttgtttac
     1021 tgagagggtg cggcaatgtg gacgagcgct cggagcggtc aaggccggga gcgtatagag
     1081 gaggacgcgt gcggccgagc atatgccgcg tacggccttg acggaaccga gtgatcgtca
     1141 aagccttgag tgaggaggat gcctgcggca cccttcgagc catgtggcgg cgaacaaaaa
     1201 tgttgaagct caaactcatc ctgcgacgtg cgtatggttt tcgtaatctg cagaatatga
     1261 ccgatatgat catgctctgc tgctcagacc tggaagtggt gcttctagga gacgatgcgt
     1321 cggcccaatc ctcctcttag acaagtgtgt tctgtacaaa agggggtacc tgttacacac
     1381 aagtcccctt gaagagccac aaaaaccgac aacccaccga aggggagccg gcttttttca
     1441 tgatgcgatt atttccagaa gggagcgtca ggccttttca tcgatctccg aggcgttcgc
     1501 atccggacgt acgggttcgt catggcgcgc accgatgagc ttgggaacgg tgacgcgacc
     1561 gtcgaggaga tcctggagcg tgacgctctg caggtacttg tcaaccacgt cggagagacc
     1621 cttccagagg aaggtggcgg cgcagcctgc gcgccggctg cagtcgtcct cgggatcgaa
     1681 gcacttcatg ggcgcttcgt cagattcgac gacggcaaga atctcagcga cggtgagtgt
     1741 tgcgggattt cgcaacagcc tgtagccgcc gagcggcccg cgtcccgtct cgaccatgcc
     1801 ggcctcggcg agtgcgggcg cgatctgctc gaggtagcgc caggagatgt cctgggaact
     1861 cgcgacggtt cgaagcttga catagcctcg cctgcgggtc tcgtcggacc ttgcggcgcg
     1921 ttcgacttcg gcaagctcga tcatgaagcg cagggcgtag cgcgagcgcg tgccgatctt
     1981 gagaaagctc attggcgggg cgtcctgatg acgggatcgg gaagatccgc aacggtggag
     2041 cggaaggggc tgatgtcgat gccgccgcga cgagtgaagc agcagttgac cgacagggcc
     2101 tcgggggcga gcactcgcat gatgtcggcg tggatctgtt cgcagcactg ctcgtggaag
     2161 ccctggtggc gtcggaagga gacgatgtag gcaaggagcg cgcgtcggtc gatgcggcgg
     2221 ccgcggtagc tgatcacgat agaggcatgg tcgggctggc ccgtgacggg gcagcgcgag
     2281 cgaaggaggt tggaggagag ggtctcctca acgacctcgt cggaggcggc cttgaggagc
     2341 gaggcgtcgg gttcgtagac gctgcactcg aggtcgccgc actcggcttc gagcacgatg
     2401 ccggggacgc gggtgacggg catctcccag cgggcgaggt cgtcaataag gacctcgacg
     2461 ggtgcgccca cgcacgcgga gacgtcgcgg gcgatgaggc gggcggcctc ttcgacggag
     2521 gcgatcttcg tctgagtgag cgagccgatg tagagcttca gggacttgga ttctacaatg
     2581 caggggctcg tcgcgggcac gcggatttcg cccgcagcgg cgacgggaag gccgcgctcg
     2641 ttgatccagg tgatttcata gaggcgccag acgtcggtgc cgcggaagtc gtgcacgccg
     2701 atcgcgtcgc gtccgagcga gcgcggaatg ggcgtgagga ggtcgggacg ataggtgtcg
     2761 acgtattcgg tcgtctggcc gagcagggtg ccggcgggcg cacaggcgga catggcttct
     2821 cctgaaatgg attcctgaga gtgaatgctg ttctcgtccg cagattatag cgggacgaag
     2881 gtgaggaacc aaaatgcaaa gaggagcctg cgggctcctc tttgcttgca tgatcggacg
     2941 gatcagcgga ttacttcacg gcttccttga agttggcgga ggcggagaag cggacgacgc
     3001 ggccggccgg gatctggatt tcctgacccg tgtgcgggtt gcggccgacg cgggcggcgc
     3061 gttcggaggt gccaaacgtg ccgaagccga tgaggcgaac gtcttcgccc ttggaaacgg
     3121 cttcaatgac gacttcaccg taggcgttga cgagcttcgt cacgtcggcc ttggcgagac
     3181 cggtcttggc ctggagggcg gtgataaatt cggacttgtt catttcaatg actcctgaag
     3241 ggaccgtgag tcccgatagt gcggcccgtc ctcctgacgg acagggcccg aataccgagt
     3301 cgaacacgga aagggacccg agctcgactt gggcacaatc atagcaaagt cttctcgccg
     3361 cagcgattgc cctccaacgg atccgaaggg cttcggggcc tgcgaacgat gcctgccccc
     3421 tcttgcaatt tttccgaaaa aagggcaggc attttcgccc gaaagcgtgg tcaggagccg
     3481 atttcgagca tttcttccgc atttctgcgt agatttgcga gggttttccg ccattacgga
     3541 accgtcggac cggccgagac acctttgcga ccctgcagaa agacccaaga atttacaagc
     3601 tgctaccaat ttaaggcctg aaacaacctt ccggaattct gcaattcatt tgacgcgcat
     3661 caagttttcc ccaaaaaaag agccctgcgg cggctcgaac gtgcgtcccg cagggcttca
     3721 gagacttcct tgggctcagc agattcgcgg aagctgctcg cccgcgagcc agtcgacggt
     3781 ccggcgtccg cccatgaggg tcaccatctc gaccatgccg actgggacgt cagagtcgtc
     3841 cgtcacgcga ccgatccttg cggcgttctc gccatagggc gaggcgcgca tggcggcgag
     3901 cgccgcgtcg gcctcgtctt cggggacgat cacgatcatc ttgccttcgt tggcgacgta
     3961 tagcggatcg agtccgagga attcgcaggc gctccggact tcggggcgca cgggcacggt
     4021 gctctcctca agaaggatcc caacgttgct ctgggaggcg atttcgttga gtgtggcggc
     4081 aaggcctccg cgggtcggat cgcgcagcac gtggacgtgc ggggcggcgg agaggaccgc
     4141 ctcgaccatt tcgttcaatg gtgcgcagtc gctctcgacg gtcgtgccga agctcatgcc
     4201 ttcgcggaag gagaggatgg cggtgccgtg gtcgcccatg gtgcccgaga tgagcacggc
     4261 gtcgccggga cgtgcggcgc gccccgagat cgtgccctcc gagatctttt cgccgatgcc
     4321 ggagatcgcg atgtagacgc cgtctccgtg gcctcgctcg accaccttgg tgtcgcccgt
     4381 cacgatggcg acgcccgcct cttcgcaggt tttcgccatg gaggcgacga cggcgtcgag
     4441 gtccttgagg ggaaaaccct cttcaagaat gaagctggcg gtgagataga ggggccgcgc
     4501 accgcacatg gcgacgtcgt tgacggtgcc tgctacggag agccgcccga tgtcgccgcc
     4561 ggggaagaag aggggacgca cgacgtggcc gtcgcaggag acggccacgg ggcgggtcat
     4621 gggcggaagg agcgcgccgt cgtggccttc gtccaaccag acgttcgtga agtgccgtcc
     4681 gaagacgtcg tgaatgagct gcgcggtggc gcggccgccc gcgccgtggg tgaggtcgac
     4741 ggcgccgttc ttcatgtcga gggggcgcat gtaaaagtcg gtcatggttc ttccttcttc
     4801 ctttatcgct gatcgtcaga agtcttcgcc ttctcgggcg ctgcggcgcg gcgtcggccg
     4861 tagctccagg cggccgcaca ggcgccttcc gaggagacca tgcaggcccc gatcgggttg
     4921 gcgggattgc aggcgcggcc aaagagcggg cattgttcgg gcgtcttttc gcccctgagg
     4981 atcgcaccgc aggcgcaggc cttgttgtcc gtgacggcgc gctcggtcat ggcaaagcgc
     5041 ttttccgcgt cgaagtccgc ccattcgggg cgaagcttca gggcggactt agggaggcgt
     5101 ccgagtccgc gccactcgaa cgaatcgcga agctcgaaga cctcgtccat taggcggatg
     5161 gcggcgacgt tcccatcacg ggtgacggcg cgggtgaatt cgttttcaac ttcgtggcgg
     5221 ccgtcgttca cctgcctcac catcatcagg atggcgtaga gcatgtcgag gggctcgaac
     5281 cccgtcacaa cgacgggaag gcggttgtcg cgcgcgaaga cctcgtaggg tcccgaaccg
     5341 atgatggtcg agacgtgggc ggggcccacg aggccgtcga gaacgggcac ttctggacgc
     5401 tcctcgctca tcttcaggat gtgggtcatc gcggcgggcg tgagcacgtg gttgcagaaa
     5461 accgagaagt tcttcacgcc cgcggccttt gcggccttga ggacaacggc cgtgggcggc
     5521 gtggtcgtct cgaacccgat cgcgaagaag acgacctcgc gctctggatt ggctttcgcc
     5581 agctcaagcg catccatggg cgagtagatc atgcggatgt ccgcgccctg agccttggcc
     5641 ttgaagagcg acatgccctt gcccgccggg acgcgcatgg tgtcggcata ggtgcagagg
     5701 atcacgttcc tttcgagcgc gagctgcatc gccatgtcga tgcgcgcgac gggcagcacg
     5761 cagacgggac agcccggtcc gtggatcatg cgcacgttgg gcggcaggat gtcggtgagc
     5821 ccccagcggg cgagcacgtg ggtgtggccg ccgcagaact ccatgaagcg gtagatcctt
     5881 ttcggatcgg cctcatgcct gatctcggca gcaatgcgcc gtgcggcatc gccctcacgg
     5941 tattcgtcga cgtacttcat gatgcgtgct cctcgtcgtg cagacccgcg gccttgagcg
     6001 ccgcgagggt cctctgggcc tcctcggggt cgatgcggtt gagcgcgaag cccacgtgca
     6061 cgatgaccca gtctcccgga acggggcgct cgatgagcga gacgttgacc gtcttttcaa
     6121 tgccgccgat gtcgcagacg gcctcgccgt cgccggcgag cctgatgatc tgtcctggaa
     6181 tggcgagaca catgtcatga ttctccttgt ggttgcactc gtccgagaac ggaggaggcg
     6241 tcgagcggcg cgtactcagt cgcgcctgcg gccagtgccg agcgggccca ccaggcctca
     6301 ccgagcgaaa gtccgccgtc tcccgcgggg acgtgcagcg gcagcgctcc ggcgaggccc
     6361 ctcgaagtga gcgcctcgac gagccccttc gagagggccg cgttgttcat ggtaccgccc
     6421 gtgagggcga caagcatgtt ttcattgtcg tccgaggcaa gaagccccgg accgaattct
     6481 tcgacgagct tcgtcgcgtg gaaggcgatc cagtcggcga gcgcgccgat gacggcattt
     6541 tccacgagag ccgcgagaca ctcgacggat gtgccagaga gcctttcgtc gacgacgcgg
     6601 cgaacgagcg gcacgagcga gagcgtg
//
LOCUS       DBMDTI010000099         6593 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_2997_length_6593_cov_2.589957, whole genome shotgun sequence.
ACCESSION   DBMDTI010000099 DBMDTI010000000
VERSION     DBMDTI010000099.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2997_length_6593_cov_2.589957"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            121..813
                     /locus_tag="ACZPB6_05945"
     CDS             121..813
                     /locus_tag="ACZPB6_05945"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1184044.1"
                     /translation="MTGFAPKYDLFVFDWDGTVMDTTALIAKGIQHAAREMGYPVPSF
                     QDACGVIGLDWRGAILKVVPDCPESEHLRFGEIYRAWYIPHEADVILFPGMRELIVGL
                     HARGLRTAVATGKSREGLRRVFERTGLEGFFMTTQTASECLPKPNAEMLEKIGIELDV
                     PAERTVMIGDTTHDVIMAHRYGCDAAAMTYGASTLQDLIASKPAVLCPDVRALAEYLG
                     VSELVKDVDFTR"
     gene            complement(901..4278)
                     /locus_tag="ACZPB6_05950"
     CDS             complement(901..4278)
                     /locus_tag="ACZPB6_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024867.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1184045.1"
                     /translation="MRLLQLRFTNINSLAGTWEIDFTNADFRRSPLFAIIGPTGSGKS
                     TILDAVSLALYATTPRMTDTHVKFEGPDACPVMTKGEGAASAAVRFEADGREYLSRWS
                     RRTKRTGRLSEDEVELVAFASPEDRTGRVIASQKRLWESEIQRITRMSFPIFTRSVLL
                     AQGAFSNFLKAADDERANLLEKITGTDLYSAVSMKIFEKNRSARDSLDMAVARLEGMQ
                     ILSDEDRRALEQTVTELSLKVPAMEKASKELDEALRWRRGLDRLTCDLADRVRVEAAA
                     KAAEAAFEPELRKAERAEQAAKPVERHEELLKTEVLFTRNKSELAEAESQLEAVRTAL
                     PDLEATLKTAATAERGAAATRDGFMPEYRRMLEADEGIRRLSHQASTAQKTAEAAFEA
                     EKTACADLETAAARQKKAADESARLQERMTTTSADAQLETPLALLKQSAAAIEALSTR
                     LRQEEQNADTLVGRLELGRVKTASFEAEVKAAVEAHAACQTTLEQARKTLAAMQAKSS
                     YEATLLRMKTLTEEMALARHVETLLQQKATIEASTARMAASADPIWWEASGAPEMQGL
                     LHRLEERLDAIEKRHPGLCTVLSTPDNTHLKSLSEERNALDKWTSDLAEGERACRAAE
                     LKEREALENKSLSAERFRKAQALLEKLEGELRHTRENRDAASKALEAEKTHAQELRSQ
                     LPDSDAELDLPALCTALEARVANRRKLEAQASQAADALTRADRTLAVCQSDAARATQA
                     KNTAEAACGAAKKACTDAVALRAEAFGERNPEAELTAHDRALNEARARLHVAQDALSK
                     AQANEREGTARAETLRKTSADLDAQITEKRTALAKALEDADFETIDEARGAALPAETI
                     RACRTQMQKLVEERVRLSGEVAQLDRQVKEESAKSLTTETAEALAPKAANAATKLLAG
                     RDELSAAKKSMAEDDRRRLEADDARREIERLRELAGQWEQLNMLLGSHDGKKFRAAAQ
                     KITFRILLKLANEAMKTMSPRYQLRTGGPSGLSLDVIDHEMGSQVRTSQNLSGGESFM
                     VSLALALGLSRMGGRNLRVDTLFLDEGFGTLDEDTLNKALYALETLQKSSGKLIGIIS
                     HVKSIRERIDAQIVVTKRPGSGRSTLSGPGVTKIDAE"
     gene            complement(4275..5579)
                     /locus_tag="ACZPB6_05955"
     CDS             complement(4275..5579)
                     /locus_tag="ACZPB6_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004529 - DNA exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HIR1184046.1"
                     /translation="MPKLRIFHSADWHIGKGLGTIDRTDDFRVFIRDFLELVEKRRPD
                     VILLAGDIFDTSMPANSAQRLYYDMIRALSGTSVMATVITAGNHDSQRFLEAPRALLE
                     TMNCFVAGESPESQAFVLRDDAGEPLLAVAAVPFLREGDVRGGSIDDTDATRAARFES
                     GVRKHYDTVNGFLDEALDGARVPRIAMGHLFVTGSRLRPDDDPTTPNESAYVGSLRNV
                     TADAFGTDWDYVALGHIHNSQTVKAQIPMRYSGSPVSLSYSHIPYRHHIVELTFDETG
                     GMTFDELPVRQPREFVRVSGTFEELRADIAEAGATHEKPFVEATLTSDECVPDLAGEL
                     TAWGEAHGVIVTAVRNQQAARKYEEDELNAPSLDDLTPESVFKSFLEERFETREGADD
                     AYRKLMPLLDEAVEEVRIETRPAIESADTPNQEKNTAEEVPA"
     gene            complement(5660..>6593)
                     /locus_tag="ACZPB6_05960"
     CDS             complement(5660..>6593)
                     /locus_tag="ACZPB6_05960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184047.1"
                     /translation="VPIRTRTAVKRITTDAEGRVNGVVVLNTKGREYRIDAPAVVIAT
                     GGFSANEAMVSRFARHTQGLQSTNSPAATGDGILLAENMGARTVDMHAVTVHPTTLPF
                     SGLILPHQVRVNGAILVNDKGERFADELSEKLAEQIRDKNSGRAWLIFDQAMIDDMPV
                     LKSYARSGYFIRGTSDLELARGIRVPPEKFHETLVRYRSMVDRQEDTDFGRPQLLSRL
                     DSSPLYAVSVRPGIHTTLGGLKIDPRARVLSDKGPIAGLYAAGEVTGGVLGARRLEGA
                     GLTAAIVYGRIAGTEAADWAKLRAKTKPAAGTGG"
BASE COUNT         1036 a         1936 c         2180 g         1441 t
ORIGIN      
        1 acgccgcccg cctgcgcttc acgcatcccg tgacgggcga gcagctcgac attcaggcgc
       61 cgatgcctga ggactgtgct gcgcttctaa aggttcttga taagaaggga ggccgctaac
      121 atgacgggct ttgcacccaa gtacgatctt tttgtctttg actgggacgg caccgtcatg
      181 gacacgacgg ctctcatcgc gaagggcatc cagcatgccg cgcgcgaaat gggctatccc
      241 gtgccgtcct ttcaggacgc ctgtggcgtg atcggcctcg actggcgagg cgccatcctc
      301 aaggtcgtgc ctgactgtcc cgagtccgag cacctgaggt tcggtgaaat ctaccgcgcg
      361 tggtacatcc cgcacgaggc ggacgtgatc ctcttccccg gcatgcgcga gttgatcgtt
      421 ggacttcatg caagaggcct caggacggcg gttgcaacgg gcaagagtcg tgaaggcctt
      481 cggcgcgtat tcgagcgtac gggattggaa ggcttcttca tgacgacgca gacggcgtcc
      541 gaatgtcttc ccaagcccaa tgccgagatg ctcgagaaga tcggcattga gcttgatgtt
      601 cctgccgaac gaacggtcat gatcggcgat acgacccacg acgtgatcat ggcgcaccgc
      661 tacggctgcg atgctgcggc catgacttac ggcgcatcga cgcttcagga cctcatcgcc
      721 tcgaagcctg ccgtgctttg ccctgacgtc agggcgcttg ccgaatactt gggagtgtcc
      781 gagttggtga aggacgttga ctttacacgc tgagtgtttt ctcacctgac ttgaagaagc
      841 cctgcggggg tgatcctgca gggcttcgct ttcttttgag cttgttgtgg aactgtacag
      901 ctattctgcg tcaattttcg tgactccagg tcctgacagc gtgcttctgc cgcttccggg
      961 acgcttcgtc acgacgatct gcgcatcaat gcgttcacga atgctcttga catgggagat
     1021 gatgccgatg agcttgccgc tcgacttctg gagcgtctcg agggcgtaaa gcgctttgtt
     1081 gagcgtgtcc tcgtcgagag tgccgaagcc ttcgtcgagg aacagtgtgt cgaccctcag
     1141 atttcggccg cccatgcgtg agagcccgag ggcaagcgcc agactcacca tgaagctttc
     1201 gccaccggag aggttctggg aggtgcgcac ctgacttccc atctcgtggt cgatgacgtc
     1261 gagagaaagt cccgagggcc cgcccgtcct gagctggtag cggggcgaca tggtcttcat
     1321 ggcctcgttg gcgagcttga ggaggatgcg gaacgtgatc ttctgggcgg ccgccctgaa
     1381 cttcttgccg tcatggctgc cgagcagcat gttgagctgc tcccactgac cggcaagttc
     1441 acgaagccgt tcgatttccc tgcgtgcgtc gtcggcttca agtctacgac ggtcatcctc
     1501 ggccatcgac tttttcgctg cgctcagttc atccctgcct gcaaggagct tggtggcggc
     1561 attcgccgcc ttgggcgcaa gcgcttcggc cgtctctgtt gtcagagact tggcggattc
     1621 ttccttcacc tgtctatcaa gctgcgcgac ctcgccggaa agacgcacgc gctcttcgac
     1681 gagcttttgc atttgcgttc tgcaagctct gatcgtctca gccggcagag cggcgcctct
     1741 ggcttcgtcg atcgtttcaa aatctgcatc ctcaagtgcc ttggcaagag cagtgcgctt
     1801 ctctgtgatc tgggcgtcca gatccgccga ggtttttctg agcgtttcgg cacgggcagt
     1861 gccttccctt tcgtttgcct gcgcctttga gagagcgtct tgcgcgacgt gcaggcgggc
     1921 gcgggcctcg ttgagggccc gatcgtgagc ggtgagctct gcttcgggat tgcgttctcc
     1981 gaaggcttcc gctctcagag cgacagcgtc ggtgcatgcc ttcttcgctg cgccgcacgc
     2041 tgcctccgcg gtgttcttcg cttgtgttgc cctggcggca tcggactgac aaacggcaag
     2101 cgtacggtcg gcgcgggtga gtgcgtcggc agcctggctt gcctgagctt cgagcttcct
     2161 tcggtttgca acgcgggctt cgagcgcggt gcagagcgcg gggagatcga gttcggcgtc
     2221 gctgtcagga agctgtgagc gaagttcctg agcatgcgtc ttttcagcct ccaaagcctt
     2281 ggaggcggcg tcacggttct cgcgggtatg cctgagctct ccctcaagtt tttcgagaag
     2341 agcctgcgcc ttccggaacc tttctgcgga taggcttttg ttttccagcg cctcgcgctc
     2401 cttgagctct gcggcacggc aggcgcgctc gccttctgca agatcgctcg tccacttgtc
     2461 cagtgcgttt cgttcttcac tgagagactt gaggtgcgtg ttgtcaggtg tcgacagcac
     2521 ggtgcaaagt cccggatggc gcttctcgat ggcatcaagg cgctcttcga gacggtgcag
     2581 aagcccctgc atttcgggag cacccgaggc ctcccaccag atggggtcgg ccgatgcggc
     2641 catgcgtgcc gtgctagcct caatcgtggc cttctgctga aggagcgtct cgacgtggcg
     2701 tgcgagtgcc atctcttcgg tcagagtctt catgcgaagc agtgtggctt cgtaggagct
     2761 ctttgcctgc atggcggcaa gggtcttgcg agcctgttcc agagtggtct gacacgcggc
     2821 atgggcttcg acggcggcct tcacctctgc ttcgaatgac gccgtcttga cgcggccgag
     2881 ctcaagacgt cccacgaggg tgtcggcatt ctgctcctcc tgccgcaggc gggtggagag
     2941 cgcttcaatc gcagcggcag actgcttcag aagcgcaagc ggagtctcga gctgtgcatc
     3001 ggcagaagtg gtggtcatgc gttcctgcag gcgggcggac tcatcggcgg ccttcttttg
     3061 tcgggcggct gctgtttcga ggtccgcgca tgcggtcttc tcagcctcga aggctgcttc
     3121 ggcagtcttt tgtgcggttg acgcctgatg agagagtcgt ctgatgcctt cgtcagcctc
     3181 gagcatgcgg cggtattcgg gcatgaagcc gtctctggtg gctgcggctc ctcgctccgc
     3241 cgtcgcggcg gtcttcaggg tagcttccag atcagggagg gcagtgcgta ccgcttcaag
     3301 ctgagattcc gcttcggcca gttcggactt gttgcgtgtg aacagaacct cggtcttgag
     3361 aagctcttca tggcgctcga caggctttgc ggcctgctcg gcgcgttcgg ccttcctgag
     3421 ctcgggctcg aaggcagcct cggccgcctt tgcggcggct tcgaccctga cgcggtctgc
     3481 aaggtcgcag gtcagtctgt cgagtcccct gcgccagcgc agtgcttcgt ccagctcttt
     3541 cgaggctttt tccatggcgg gcaccttgag cgagagctcg gtcaccgttt gttccagcgc
     3601 ccgacgatcc tcgtcggaga gaatctgcat gccctcgagt ctggcgacgg ccatgtcaag
     3661 actgtcgcgg gcgcttcggt ttttctcgaa gatcttcatg ctgacggccg aataaagatc
     3721 cgtccccgtg atcttttcga ggagatttgc acgctcgtcg tcggcagcct tgaggaagtt
     3781 ggagaaggcg ccctgcgcaa gcagcaccga gcgggtgaag atcgggaagg acatgcgcgt
     3841 gatgcgctgg atctcgcttt cccacaggcg cttctgactg gcgatgacgc gtcccgtcct
     3901 gtcttcgggg cttgcgaagg ccacaagctc gacttcgtct tcggaaagcc tgcccgtacg
     3961 cttcgtgcgc cggctccatc gcgacaggta ctcgcggccg tccgcctcga atcgaacggc
     4021 ggcgctggcc gcgccttccc ccttcgtcat gacggggcag gcgtcgggtc cctcgaactt
     4081 gacgtgtgtg tccgtcatgc ggggcgtcgt ggcataaagc gcgagcgaga cggcgtcgag
     4141 aatcgtcgac ttgccgctgc ccgtcgggcc gatgatggca aaaagcggac tgcgcctgaa
     4201 gtcggcgttc gtgaagtcga tctcccaggt gcccgcaagt gagttgatgt tcgtgaaccg
     4261 gagttgcagg agcctcatgc cggaacctcc tcggcggtgt tcttctcttg gttgggtgtg
     4321 tcggcgcttt cgatggcggg gcgcgtctcg atgcgcacct cctcgaccgc ttcgtcgaga
     4381 agcggcatga gcttgcgata ggcgtcgtct gcgccttcgc gcgtttcaaa gcgctcctca
     4441 agaaaggact tgaagacgga ttcgggcgtc aggtcgtcga gggacggcgc attgagctcg
     4501 tcctcttcgt atttgcgtgc ggcctgctgg ttgcgtacgg cggtgacgat gacgccgtgc
     4561 gcctcgcccc aggcggtgag ttcgcccgca aggtcgggca cgcattcgtc cgacgtgagc
     4621 gtcgcttcga cgaagggctt ttcatgtgtt gcgccggcct cggcaatgtc tgcgcgaagc
     4681 tcctcgaacg tgccgctgac acgaacgaat tcacggggct gcctgacggg aagttcatcg
     4741 aaggtcatgc cgcccgtttc atcgaaggtg agctcgacga tgtgatggcg gtacgggatg
     4801 tgcgagtagg aaagcgagac gggagagccg ctgtagcgca tgggaatctg tgccttgacc
     4861 gtctggctgt tgtgaatgtg tccgagggcg acgtagtccc agtccgtgcc gaaggcgtcg
     4921 gccgtgacgt tgcgaagaga gcccacgtag gcactctcgt tcggtgtcgt cgggtcgtcg
     4981 tcgggtcgca ggcgcgatcc cgtcacgaac aggtgcccca tggcgattcg gggtactcgt
     5041 gcgccgtcga gcgcctcgtc cagaaagccg ttaacggtat cgtagtgctt cctgacgccc
     5101 gactcgaatc tggcggccct cgtggcgtcc gtgtcgtcga ttgagccgcc tcttacgtcg
     5161 ccctcgcgca ggaagggaac ggccgcgacg gcaaggaggg gctcgcccgc atcgtctcgg
     5221 agaacgaacg cctgactttc gggcgactcg cccgcgacaa agcagttcat cgtttcgaga
     5281 agtgccctgg gggcttcgag aaagcgctgt gagtcgtggt tgcccgccgt gatgacggtg
     5341 gccatgaccg acgtgcccga gagggcgcgg atcatgtcgt aatagaggcg ctgggcactg
     5401 tttgcgggca tggacgtgtc gaagatgtcg cccgctagaa gaatgacatc gggcctgcgc
     5461 ttctcgacga gttcgagaaa gtcgcggatg aagactcgga agtcgtccgt gcggtcgatc
     5521 gttccgagtc ccttgccgat gtgccagtcg gccgagtgaa agatgcgaag ctttggcaaa
     5581 ggaagggcct ccgtgggttc tgtggaacca ttatgacgga agccgcgtca ggttgcgtcg
     5641 tctggcgacg gagtggccct cagccgcccg ttccagccgc aggcttcgtc ttcgcgcgca
     5701 gctttgccca gtctgcggct tcggtgcccg cgatgcggcc gtagacgatg gctgcggtaa
     5761 gacctgcacc ctcaaggcgt ctggcgccga ggacgccgcc cgtcacttcg cccgcagcat
     5821 agagcccggc aatggggccc ttgtcgctca ggacgcgggc gcgcgggtcg atcttgaggc
     5881 cgccgagtgt cgtgtgaatg ccggggcgca cgctcacggc gtagagcggg gagctgtcga
     5941 ggcggctcag gagctgggga cggccgaagt ccgtgtcctc ctgacggtcg accatgctac
     6001 ggtagcgcac gagggtttcg tggaactttt cgggaggcac gcggatgcct cgggcgagtt
     6061 caaggtcgga cgtgccgcga atgaagtagc ccgagcgggc gtagctcttg agaactggca
     6121 tgtcgtcgat catcgcctga tcgaagatga gccaggcgcg gccgctgttc ttgtcgcgga
     6181 tctgctccgc cagtttttcg gaaagctcgt cggcgaagcg ttcgcccttg tcgttgacga
     6241 ggatcgcgcc gttgacgcgc acctgatgag gcaggatgag accggaaaaa ggaagtgtgg
     6301 tcggatgaac cgtcacggcg tgcatgtcga ctgttcgggc ccccatgttc tcggcaagaa
     6361 ggatgccgtc gcccgtggcg gcgggggagt tggtggactg caggccctgc gtgtggcgtg
     6421 cgaaacggga gaccatcgcc tcgtttgcgc tgaagccgcc cgttgcgatg accacggcgg
     6481 gcgcatcaat cctgtactcc ctgcccttcg tgttgaggac gacgacgccg ttgacgcggc
     6541 cctcggcgtc ggttgtgatt ctcttgacgg cggtgcgggt gcggatgggc aca
//
LOCUS       DBMDTI010000100         6539 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3022_length_6539_cov_4.531221, whole genome shotgun sequence.
ACCESSION   DBMDTI010000100 DBMDTI010000000
VERSION     DBMDTI010000100.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3022_length_6539_cov_4.531221"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2138)
                     /gene="rpoB"
                     /locus_tag="ACZPB6_05965"
     CDS             complement(<1..2138)
                     /gene="rpoB"
                     /locus_tag="ACZPB6_05965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR1184048.1"
                     /translation="MSYSFTEKKRIRKSFASRPSVLEVPSLLDTQLRSYEEFLQADVA
                     PNKRNTEYGLQAAFTSIFPITSHNGYARLRFVDYELAEPEFDVAECQLRGLTFCSRLR
                     AKIQLEIFDRDAAKPETLKEIRENDVYMGEIPLMTEKGSFIINGTERVIVSQLHRSPG
                     IFFEHDKGKTHSSGKLLFSARVIPYRGSWLDFEFDAKDILYFRVDRRRKMPGTILLKA
                     LGLTPEDILARFFTFDEITINANGASLPFVPERLKGQTVSFPIIADGKEVVPADKRIT
                     AKHIRDLTKAGVTSIPVPDEYVLGRVLGKNIVDEETGEVIANCNDEITPDLLSQLRVA
                     KIKTIFTIFTNELDHGSYISSTLRLDDTPDQLAARVAIYRMMRPGEPPTEDAVEALFN
                     RLFFDAEAYDLSRVGRMKVNARFGREGATGSMTLTREDIIDTMAVLVALRNGQDTVEL
                     FDADGVARTCVVNGVDDIDHLGNRRVRCVGELAENQFRSGLVRVERAVKERLNQAESD
                     GLTPQDLINSKPISAAIREFFGSSQLSQFMDQTNPLSEITHKRRISALGPGGLTRERA
                     GFEVRDVHPTHYGRVCPIETPEGPNIGLINSLALYARLNEYGFLETPYRKVVDGVATQ
                     EISYLSAIEEGRYIIAQANAEMDENGRLTQELVSARDNGETVLASPDRIQYMDVAPSQ
                     IVSCAAALIPFLEHDDANRALMGSNMQRQGV"
     gene            complement(2414..2791)
                     /gene="rplL"
                     /locus_tag="ACZPB6_05970"
     CDS             complement(2414..2791)
                     /gene="rplL"
                     /locus_tag="ACZPB6_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR1184049.1"
                     /translation="MALTNEEILEAIASKTVLEISELIKMMEEKFGVSAAAAAVAVAA
                     PAAGAAAAAEEKTEFDVVLESAGSNKIAVIKVVREMTGLGLKEAKDLVEGAPKAVKEG
                     LPKADAEAAAKKLEEAGAKVALK"
     gene            complement(2838..3368)
                     /gene="rplJ"
                     /locus_tag="ACZPB6_05975"
     CDS             complement(2838..3368)
                     /gene="rplJ"
                     /locus_tag="ACZPB6_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR1184050.1"
                     /translation="MGLNRQDKAAVIEEVAGIVANSSSIVIAEYRGLSVEAVTKLRAE
                     ARKNNVTLRVVKNTLVRRAVEGTEFAGLADQFVGPLVYGFSADPVAAAKVLVNFAKDN
                     DKLVIKGGAMANTVMDVEAVKQLATMPSREELLAKLMATMNEPIAKFVRTINEVPARF
                     VRTVAAVRDAKEQAAA"
     gene            complement(3812..4507)
                     /gene="rplA"
                     /locus_tag="ACZPB6_05980"
     CDS             complement(3812..4507)
                     /gene="rplA"
                     /locus_tag="ACZPB6_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR1184051.1"
                     /translation="MAKLCKRMKALNAKVEANKLYTAVEALELIKETAVAKFDESVDV
                     AVQLGIDPRKSDQAVRGAVVMPEGTGKTVRVAVFTQGAKAEEALAAGADIVGFDDLAA
                     KVKAGELDFDVVIASPDAMRVVGQLGQILGPRGLMPNPKVGTVTPNVAQAVKNAKAGQ
                     VQFRADKAGIIHAPIGRASFTADRLQKNLVALIDQLNKLKPASSKGQYLRKVAVSSTM
                     GIGVRVDIGSINN"
     gene            complement(4507..4938)
                     /gene="rplK"
                     /locus_tag="ACZPB6_05985"
     CDS             complement(4507..4938)
                     /gene="rplK"
                     /locus_tag="ACZPB6_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR1184052.1"
                     /translation="MAKKIIGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     KTQGMEAGLPIPVVITAYADKSFTFIMKTPPATILIKKAAKIQKGSSRPNTEKVAKIT
                     RAQAEEIAKTKAPDLTAADIDAAVRTIAGSARSMGIIVEGL"
     gene            complement(5057..5593)
                     /gene="nusG"
                     /locus_tag="ACZPB6_05990"
     CDS             complement(5057..5593)
                     /gene="nusG"
                     /locus_tag="ACZPB6_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR1184053.1"
                     /translation="MSDNGMKWYTIHVYSSMEKSVKKALEERIGRSDLRESFGQVLLP
                     VERVQEVRNGRKYTSERCMYPGYVFIEMVMNDATWHLVNSTPRVIEFLGNNNPVVLSE
                     AEVASIMAQMEQGSEKPRPKIEFEIGEEIRVKNGPFKDFNGRIEAVDYDKSRLQVFVS
                     IFSRETLVDLDFNEVEKI"
     gene            complement(5608..5991)
                     /gene="secE"
                     /locus_tag="ACZPB6_05995"
     CDS             complement(5608..5991)
                     /gene="secE"
                     /locus_tag="ACZPB6_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR1184054.1"
                     /translation="MNNQNVEPATSKSDIVLVTLAVLCAVAGVIAFSLLTSQTLAVRL
                     GALFGGLVAGVVLAWFSPSGKRLLNYGRQSWEELRRVVWPTRKETLNTTGLVMAFVVV
                     IAIFLFLCDKLIEWGLYDGLLRLSL"
     gene            complement(6171..6246)
                     /locus_tag="ACZPB6_06000"
     tRNA            complement(6171..6246)
                     /locus_tag="ACZPB6_06000"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6211..6213),aa:Trp,seq:cca)
     gene            complement(6294..>6539)
                     /locus_tag="ACZPB6_06005"
     CDS             complement(6294..>6539)
                     /locus_tag="ACZPB6_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR1184055.1"
                     /translation="KEEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNI
                     QMTVKLICPIAMEQGLRFAIREGGHTVGAGVVAAVIE"
BASE COUNT         1364 a         1773 c         1998 g         1404 t
ORIGIN      
        1 acgccctgac gctgcatgtt cgaacccatc agagcacggt tcgcgtcgtc gtgttccagg
       61 aacggaatca aagcggcggc gcaggagacg atctgagacg gggccacgtc catgtactgg
      121 atgcggtccg ggctggcgag aaccgtttcg ccgttgtcac gggcggaaac gagttcctgc
      181 gtcagacggc cgttctcgtc catctcggcg ttggcctgag cgatgatgta gcggccttcc
      241 tcaatggcgg acaggtagga gatttcctgc gtcgcgacgc cgtcgaccac cttgcggtac
      301 ggcgtttcaa ggaagccgta ttcgttgagg cgggcgtaaa gggcgagcga gttgataaga
      361 ccgatgttcg gaccttcagg cgtttcaatt gggcagacgc gaccgtagtg ggtcggatgc
      421 acgtctcgga cttcgaagcc ggcgcgttcg cgagtcagac cacccgggcc aagggccgaa
      481 atacggcgct tgtgagtgat ttccgacagc gggttcgtct ggtccatgaa ctgggacagc
      541 tggctcgagc cgaagaactc gcggatggcg gcgctgatcg gcttggagtt gatcagatcc
      601 tgcggcgtga ggccatcgct ttcagcctgg ttgagtcgtt ccttgacagc tcgctcaacg
      661 cgcacgaggc cggagcggaa ctgattttcg gcgagttcgc cgacacagcg gacacgacgg
      721 ttgcccaggt ggtcgatgtc gtcgacgccg ttgaccacgc aggtgcgggc cacgccgtcg
      781 gcatcaaaga gctcgacggt gtcctgaccg ttgcgcaggg caacgaggac ggccatcgtg
      841 tcgatgatgt cttcgcgggt aagcgtcatg gaacccgtag cgccttcacg gccgaaacgg
      901 gcattgacct tcatgcggcc gacgcgggac agatcgtacg cttcggcatc gaagaagaga
      961 cggttgaaca gagcctcgac agcgtcttcc gtgggcggtt cgcccggacg catcatgcgg
     1021 tagatcgcga cgcgtgcggc gagctgatcc ggcgtgtcgt ccagacgaag cgtggacgag
     1081 atgtaggaac cgtggtcgag ttcgttcgtg aagatcgtga agatcgtctt gatcttggcg
     1141 acgcggagct gggaaagaag atcgggcgtg atttcgtcgt tgcagttggc aatgacttca
     1201 cctgtttcct cgtcaacgat gttcttgccg agaacgcggc cgagaacata ttcgtcggga
     1261 acgggaatgc tcgtcacgcc ggccttcgtc aggtcgcgga tgtgcttggc ggtgatgcgc
     1321 ttgtcggcgg gcaccacttc cttgccgtcg gcaatgatcg ggaagctgac ggtctggccc
     1381 ttcaggcgtt cgggaacgaa cggaagggag gcgccgttgg cgttgatcgt gatttcgtcg
     1441 aacgtgaaga agcgcgcaag gatgtcttcc ggcgtcaggc cgagggcctt gagcagaatc
     1501 gtgccgggca tcttgcggcg gcggtcgacg cggaagtaca ggatgtcctt ggcgtcgaat
     1561 tcgaagtcaa gccaggagcc gcggtaagga atcacgcgag ccgagaagag cagcttgccg
     1621 gaggagtgcg tcttgccctt gtcatgttca aagaaaatgc cgggcgaacg gtgcagctgg
     1681 ctgacgatga cgcgttccgt gccgttgatg atgaacgagc ccttctccgt catgagcggg
     1741 atttcgccca tgtagacgtc attttcgcga atttccttca gcgtctcggg cttggcggca
     1801 tcgcggtcaa agatttccag ctggatcttg gcgcgaagac ggctgcagaa cgtcaggccg
     1861 cggagctggc actcggcgac gtcgaattcc ggttcagcaa gctcgtaatc gacgaagcga
     1921 agacgtgcgt aaccgttgtg gctggtaatc gggaagatgg aagtaaacgc tgcctgcaaa
     1981 ccgtattcgg tattgcgctt gttcggggca acgtccgcct gcagaaattc ttcgtaggaa
     2041 cgaagctgag tatcaagcag ggaaggcact tcaaggacgc tcgggcgaga ggcaaagctc
     2101 ttgcggatgc gcttcttttc agtgaacgag tacgacatgg acactccgtt cgacattgag
     2161 ggatgaacca aaaattgatg aacgcaaact ctatggacgc caaaagccgc tcgggaagca
     2221 caacctgtcg gttgaacccg cgcggcggat atggggatat ttcaagttaa gcggttcatc
     2281 aaagaagaaa gactttgaac tccattctcc atcctgggag aacatagttc aaagtctccc
     2341 tttcgggaga ctctgaaaaa caaagcgagc gactttaatt gtgctcgctt tgttcagtca
     2401 aatcaagtaa aaattacttg agagcgacct tggcgccggc ttcttcgagc ttcttggcag
     2461 cagcttcggc gtcagccttc gggaggcctt ccttgacagc cttcggagcg ccttcgacga
     2521 ggtccttggc ttccttgagg ccgaggccgg tcatttcgcg aacgaccttg atgacggcga
     2581 tcttgttgga accggcggat tcgagaacga cgtcgaattc cgtcttttct tcagcagcgg
     2641 cggcagcgcc agcggccggg gcggcaacgg cgacagcagc ggcagcagcg gaaacgccga
     2701 acttttcttc catcatcttg atgagttcgg agatttcgag aacggtcttg gaagcgatcg
     2761 cttcgaggat ttcttcattg gtaagggcca ttttatagct ccgtattacg tagtgttttg
     2821 tttaaaaaaa gtttaggtta ggcagcagcc tgttccttgg catcgcgcac ggcagccacg
     2881 gtacgcacaa agcgggccgg gacttcgttg atggtgcgga cgaacttggc gataggttcg
     2941 ttcatcgtag ccatcaactt ggcaaggagt tcttcacggc tcggcatcgt agcgagctgc
     3001 ttgacggctt caacatccat gacggtgttg gccatcgcgc cgcccttgat aacgagcttg
     3061 tcgttgtcct tggcgaaatt gacgagaacc ttggcggcag caaccgggtc ggcggagaag
     3121 ccgtagacga gcggaccaac gaactggtca gcgagtccgg caaattccgt accttcgacg
     3181 gcgcggcgaa ccaacgtgtt cttgacaaca cgcaacgtca cattgttctt acgtgcttca
     3241 gcacgaagct tcgtgacggc ctcaacgctc agcccacggt attcggcgat gacgatcgag
     3301 gaagagttgg caacgatgcc agcgacttct tcaatgacag cagccttatc ttgacgatta
     3361 agacccatgg tctggctcca ctcagaggtg tccccttgcg aggacaccgg ttttcaaata
     3421 accggcatac ttctgagagt tttggctgat tttttacaca agcacacgag cttgtggtct
     3481 gaaacacaac gcaatatctc tcttcggttt accgtctgcg ctggacgact tggcggtcat
     3541 cccactgccg tgaaagcatc ggcagtgaag ttcacaagcc cattaaaaag cgccggtgtc
     3601 aatctcttcg ttgctttcag agaattgttg acctttgctt ctccagcggt ctttgacagc
     3661 gacgggcccg attgctcggc cccgtcaccc aaagcccttg agacctcacg gtctcgagaa
     3721 aagatttctc ttttccggtc tcgccgcttt cgcagcgtcc tgatcaggtt gccctgacca
     3781 acggaggacc gaccccgtta tgtttggttc attagttgtt gatagaaccg atatcaacgc
     3841 gaacaccgat gcccatcgtg gaggagacgg caaccttgcg gaggtactgg cccttcgagg
     3901 aagccggctt cagcttgttg agctggtcga tgagagccac caggttcttc tggaggcggt
     3961 cagccgtgaa ggaggcacga ccgatcgggg cgtggatgat accagccttg tcggcgcgga
     4021 actggacctg accggccttg gcgttcttga cagcctgggc aacgttcggg gtgacagtac
     4081 cgaccttcgg gttcggcatc aggccgcgcg ggccgaggat ctggccgagc tggccgacaa
     4141 cgcgcatcgc atccggggag gcgatgacga cgtcgaagtc gagctcaccg gccttgacct
     4201 tggcggcgag gtcgtcgaaa ccgacgatat cggcgccggc ggcaagagct tcttcagcct
     4261 tggcgccctg ggtgaacacg gcaacgcgaa cggtcttgcc cgtgccttcc ggcatcacga
     4321 cagcgccgcg gacggcctgg tcggacttgc gcggatcgat gccgagctgg acggccacgt
     4381 cgacagattc atcgaacttg gcaacagccg tttccttgat gagttcgaga gcttcaacag
     4441 cggtgtaaag cttgttggct tcgaccttgg cgttgagagc cttcatgcgc ttgcaaagct
     4501 tagccattac agaccctcca caatgatgcc catggaacga gcggaaccag cgatcgtacg
     4561 aacagcagcg tcgatgtcgg cagcggtaag gtccggagcc ttcgtcttgg cgatttcttc
     4621 agcctgagcg cgcgtgatct tggcgacctt ttccgtgttc ggacgggaag aacccttctg
     4681 gatcttggca gccttcttga tcaggatcgt ggccggcggg gtcttcatga tgaacgtgaa
     4741 gctcttgtcg gcgtacgcgg tgatgacgac cgggatcgga agaccagctt ccatgccctg
     4801 cgtcttagcg ttaaaggcct tgcagaactc catgatgttc agaccgcgct gaccgagagc
     4861 cggaccgata ggaggcgagg ggttggcctt accagccggc acctgcagct tgataaagcc
     4921 gatgatcttc ttagccatcg ttaaatttcc ttatgggttc atgcgctcaa ttgcttgagc
     4981 tccccatgta tgtgtgagaa aggcgtgcac tataccaccg aaaaaatcgg tgtgcaagcg
     5041 acacgccaga atttttttag attttttcga cttcgttgaa gtcgaggtcc accagcgttt
     5101 cgcggctgaa aatggacacg aagacctgga gacggctctt gtcgtagtcg acggcctcga
     5161 tgcggccgtt gaagtccttg aacgggccgt tcttgacgcg gatctcctcg ccgatctcga
     5221 attcgatctt gggacgcggc ttctcggagc cctgctccat ctgagccatg atggaggcca
     5281 cttcagcctc ggaaagcacc acggggttgt tgttgccgag gaactcaatg acgcgtgggg
     5341 tggagttgac gagatgccac gtagcgtcgt tcatcaccat ttcgatgaag acgtagccgg
     5401 ggtacatgca gcgctcgcta gtgtacttgc gaccgttgcg gacttcctga acgcgttcaa
     5461 cgggcaggag aacctgaccg aagctttcgc gcaggtcgct gcggccaata cgctcctcga
     5521 gagccttctt cacgctcttc tccatggaag agtagacgtg aatcgtgtac cacttcatgc
     5581 cgttatcgct catgactcaa cctcacatta aagagaaaga cggagcaggc cgtcgtagag
     5641 accccactca atcagcttgt cgcaaaggaa aaggaagatc gcgatcacga cgacgaaggc
     5701 catgacgagg cccgtcgtgt tgagcgtttc cttgcgggtc ggccagacga cgcggcgcag
     5761 ttcttcccag gactggcggc cgtagttcag aaggcgcttg ccggaggggc tgaaccaagc
     5821 gagcacgacg ccggcaacca gaccgccgaa gagcgcgccg agacggacgg cgagcgtctg
     5881 ggacgtcagg agggaaaagg caataacgcc ggcaacggca cacaggacgg cgagcgtcac
     5941 cagcacgatg tccgacttgc tggttgcggg ttcaacgttt tgattgttca tattcattcc
     6001 tgccaacacg ctcagcggcg cgccgacgaa aagtaaaaga aatgcaagct tgctatttta
     6061 gcggaaaccg aagccatata ggcgaaaaat tcccgacttt ctgcgctttc agattccgca
     6121 gatagataaa agtcaagccg cgatcacaga tcgcggcttg acttcttttt tggcaggggc
     6181 agaaggaatc gaaccctcaa cctacggttt tggagaccgt cgctctgccg attgagctat
     6241 accccttttc gtgcggccgg ccctttcagg tcagccgcca attcttttca gaattattcg
     6301 atgacggcag caaccacgcc agcgccgacg gtgtgaccgc cttcgcggat ggcgaagcgg
     6361 agaccctgtt ccatggcgat cgggcagatg agcttgacgg tcatctggat gttgtcgcca
     6421 ggcatgacca tttcaacgcc ttccggcagt tcgatcgtgc ccgtaacgtc cgtcgtacgg
     6481 aagtagaact gcggacgata gcccttgaag aacggggtgt gacggccgcc ttcttcctt
//
LOCUS       DBMDTI010000101         6477 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3053_length_6477_cov_3.231179, whole genome shotgun sequence.
ACCESSION   DBMDTI010000101 DBMDTI010000000
VERSION     DBMDTI010000101.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3053_length_6477_cov_3.231179"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="ACZPB6_06010"
     CDS             complement(<1..697)
                     /locus_tag="ACZPB6_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024240.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184056.1"
                     /translation="MLNSGHGHQTLGKLLSRCLRGAAGIAAGIGLAAAATSALAADAH
                     CDVVVIGAGGSGLTSALSAAEAGAKVIVVEKMPFIGGNTVLATGFMLGVPKGEPRELE
                     LLESDMIRKGGRTTDQALLTRIVSGSGEAVEWLRSYGAELEPLCITRDDSLQTACNLN
                     EDGTVSKRGIQPKRGLIGPEIINALLHGIESHGVPIRTRTAVKRITTDAEGRVNGVVV
                     LNTKGREYRIDAPA"
     gene            1194..4301
                     /locus_tag="ACZPB6_06015"
     CDS             1194..4301
                     /locus_tag="ACZPB6_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR1184057.1"
                     /translation="MEVSRRSFLKRGLLLGGGAASGLAGAAAHAAGGSKKPYKLTSVN
                     ETTNICCYCSGGCGTICSTRNGELINLEGDPDHPVNLGGLCPKGAAMWGLRNIVTKDR
                     RAQLHPDRVLYPMVRRPGSKTWERLSWDQAALEIARHVKKTRDATFVEKEGDVTVNRC
                     DGIASLGAAQLNNEEGWLVQKFARSLGVLAIDNQTRVCHSSTVSGLAPSFGRGSMTSH
                     WCDFANSDVIMSIGSNNVENHPLSSRWVERAQDKGATWIVVDPRYSRSAARADIYARI
                     RPGSDLAFYGGLINYILQNDLFQKEYVLHYTNAACLLRPDFKFDVDHGLFSGWDPETK
                     RYDNETWGYDVDHKAVWDTSEGSAFAWVNRPGTPKFKTPDLKVLKRDMTLQDPNCVLN
                     VMKRHYARYTPELVTRVTGMDPDVMKKVWEVFASTGRPDKAGSILYALGQTQHTYGSQ
                     NCRAMCVIQLLLGNIGIAGGGINALRGEPNVQGSTDVGASSHQAPAYLSWPTGKAHPK
                     LADYLSKETYAAGYYANKPKFWISQLREWFGANATVENDYCYDLLPKISPKLDYGDYS
                     TIMSFNDMRDNKIKGYFCWGMNPMHSTPNGKHARHSMANLDWLVVADWFQTETSTFWE
                     APDMKPEDVKTEVYFLPAALIYEKIGSINNSGRWIQWREKAIEPPGECRSDFEMMMLL
                     WKNIVELYKKEGGACPDQILKTNFDYTIDGKPDLRALCWALNGYTVGDGKLLKGYGQL
                     QADGTTACGMWIFSGYYNNESQKLDPMKQPMTNRSKEDPTGLGLFPGWSFAWPANRRI
                     LYNRCSADPKGKPWDPKRVLVEWDGKKWLQNDVGDFVTAKAPDDNAFFMTWEQNARLF
                     AYSMADGPLPEHFEPHEAPVKNLLNGAGGNPCARFTEDPSVRRGSIKDYPYVVTTYSV
                     VEHWQSGTQTRNIPWLNELIPSNFIELSEELAKEKGIRPGDKVRVWNNRGSVVVDAMI
                     TIRMKPMMIDGKLTHVVGMPHHFSWATKLATGDNVNDLTPNVGDPNSYIPEYKAFLVN
                     LEKAA"
     gene            4325..5221
                     /locus_tag="ACZPB6_06020"
     CDS             4325..5221
                     /locus_tag="ACZPB6_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1184058.1"
                     /translation="MANTKEVAMLFDSSHCTGCKGCQVACKQWNMLPSKLGLNENKFS
                     GTYQNPPDLNGDTRLVMTFDEKESGNKYQPINFAIGRRSCYHCTDAACVAICSSGALY
                     KKENGVVAYDTDKCNGCTYCQSACPFDVPRFRPMDGCVDKCTLCIDRLEEGGIPACVK
                     TCQPEALKFGPRDEMIALGQKRVEFLKKKGFDKAELYGVNEMGGLHVLQVCQFGHEAY
                     GLPTDPKPNKMIGMMNAMRTVTGVGTAAVLAGLAVSFVAATGYRREKVTVEEAKKHWT
                     PEQRATADREVKELLEKEASGK"
     gene            5235..5960
                     /locus_tag="ACZPB6_06025"
     CDS             5235..5960
                     /locus_tag="ACZPB6_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR1184059.1"
                     /translation="MTRYIQRHSLLTRVTHGVAAVACILLAVTGVFVFVPSLGGGIMG
                     GEFTQTMRMLHRVLAVPFILVPLFAVLKSPAGFVHLFKEDIFGKWDKDDVTWAMKFPF
                     YLFAPGKVHMPPQHHVKSAQRLADGALLFFCIMLALSGIVLWLNTGFPTGSGLKVDFA
                     PETLLLAHLVHDVFFFLTVFVGIAHIYLGAGIFQPYRGTARIMFGDGKVSESDAVYHW
                     GYWANEEIALGKNVTVEKDGKEK"
     gene            6173..>6477
                     /locus_tag="ACZPB6_06030"
     CDS             6173..>6477
                     /locus_tag="ACZPB6_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184060.1"
                     /translation="MLNRKSIERALARAAGHQDESVVARLALFAPALLKAADLRAQLA
                     AEAPEWTDEEIEAARLGKETLLSLRPVTIDSERFLQTVDAIAAALLDNDEIEGDMLEA
                     "
BASE COUNT         1283 a         2118 c         1898 g         1178 t
ORIGIN      
        1 cggcgggcgc atcaatccgg tactccctgc ccttcgtgtt gaggacgacg acgccgttga
       61 cgcggccctc ggcgtcggtt gtgattctct tgacggcggt gcgggtgcgg atgggcacac
      121 cgtgggattc gatcccgtgc agaagggcgt tgatgatttc gggcccgatg aggccgcgct
      181 tgggctggat gccgcgcttg gaaaccgtgc cgtcctcatt gaggttgcag gccgtctgaa
      241 ggctgtcgtc gcgggtgatg cagaggggct cgagttcggc gccgtagctg cgaagccatt
      301 caacggcttc gcccgagccg gagacgatgc gtgtgagaag ggcctgatcg gtcgtgcggc
      361 cgcccttgcg gatcatgtcg ctctcgagca gctcgagctc gcggggttcg cccttcggga
      421 cgccgagcat gaagcccgtg gcaagaacgg tgttgcctcc aatgaagggc atcttctcga
      481 cgacgattac cttcgcgccc gcttcggctg cggagagcgc cgacgtgaga cctgagccgc
      541 ccgcaccgat cacgaccacg tcgcagtgcg cgtcggcggc aagggccgat gtggctgccg
      601 ctgcaaggcc gatgccggcg gcaatgcccg ccgcgccgcg aaggcagcgc gaaagaagtt
      661 tgccgagggt ctgatgaccg tgtccgctgt tgagcatggt gtggtttcga gctgaagaca
      721 gtctttgaag gttgggcgtc gttcgccctt tgcaggtctc gggtaaaccc gtaggcaaaa
      781 gaatagcaaa aaaaagagaa gggcgcagtg gcgttttccg caaacgcttc gggcaggcgg
      841 acttgaaaac ctgagaatac cgtgaatgcg gtatgccggc catgtgatct ttggacaact
      901 cgctattttc attgtgaata gcggggctct cctgcagaaa tgagtttccc gttgaagccc
      961 cttcgtcaga agtacctctc aggtggtaga tgtattaaag ctaagggagg gggcttttgg
     1021 ttgaaaacta ctgcgagagt agggttgtca tgaattcgac atgagggcag actctctgtg
     1081 tcgacggagt ccccctctgt catgacgaaa atcaccgccg cgatgcgccg cccctgattc
     1141 ggtgcatcgc tcatgcgacg gcaagaacaa caactttcca agaaggatcc cccatggaag
     1201 tatctcgcag aagttttctc aagcgtggtt tgcttctcgg cggcggtgct gcgtccggtc
     1261 ttgccggcgc ggctgcccat gcggccggcg gctccaagaa gccctacaag ctcaccagcg
     1321 tgaatgaaac gacgaacatc tgctgctact gctcaggcgg ctgcggcacg atctgctcga
     1381 cgcgaaacgg cgaactcatc aatcttgaag gcgacccgga tcatccggtc aaccttggtg
     1441 gcctgtgccc gaagggcgcc gccatgtggg gccttcgcaa catcgtgacg aaggatcgcc
     1501 gcgcccagct tcatccggac cgcgtgctct atccgatggt tcgccgtccg ggcagcaaga
     1561 cctgggagcg tctttcctgg gatcaggccg ctcttgaaat tgccaggcac gtcaagaaga
     1621 ctcgcgacgc caccttcgtc gagaaggaag gcgacgtgac ggtcaatcgc tgcgacggca
     1681 tcgccagcct aggcgccgcc cagcttaaca acgaggaagg ctggctcgtg cagaagttcg
     1741 cgcgcagcct cggcgtcctc gccatcgaca accagacccg cgtctgtcac tcttcgaccg
     1801 tttccggtct tgcgccgtcg ttcggccgcg gctccatgac gagccactgg tgcgacttcg
     1861 ccaactccga cgtcatcatg tcgatcggtt cgaacaacgt cgaaaaccat ccgctctcct
     1921 cccgctgggt cgagcgcgcc caggacaagg gcgccacgtg gatcgtggtc gatccgcgct
     1981 actcccgttc ggccgcccgc gccgacatct acgcccgcat ccgtccgggc tccgaccttg
     2041 ccttctacgg cggcctgatc aactacatcc tgcagaacga ccttttccag aaggaatacg
     2101 tcctgcacta cacgaacgcc gcctgcctgc tgcgtcccga cttcaagttc gacgtcgatc
     2161 acggcctctt ttccggctgg gatccggaaa cgaagcgcta cgacaatgaa acctggggct
     2221 acgacgtcga tcacaaggcc gtctgggaca cttccgaagg ctccgccttc gcctgggtca
     2281 accgtccggg cacgccgaag ttcaagacgc ctgacctcaa ggtcctcaag cgcgacatga
     2341 cccttcagga tccgaactgc gtcctcaacg tcatgaagcg tcactatgcc cgctacacgc
     2401 ccgagctcgt cacgcgcgtc accggcatgg atcccgacgt catgaagaag gtctgggagg
     2461 tcttcgcctc caccggccgt cccgacaagg cgggctccat cctctacgcc ctcggtcaga
     2521 cgcagcacac ctacggctcc cagaactgcc gcgccatgtg cgtcatccag cttctgctcg
     2581 gcaacatcgg catagcgggc ggcggcatca acgcccttcg cggcgaaccg aacgttcagg
     2641 gttcgaccga cgtcggcgca agctcgcatc aggctcccgc ctatctctcc tggccgaccg
     2701 gcaaggctca cccgaagctc gccgactatc tttcgaagga aacgtacgcc gcgggctact
     2761 atgccaacaa gccgaagttc tggatctccc agcttcgcga atggttcggc gccaacgcca
     2821 cggtcgagaa cgactactgc tacgacctgc tgccgaagat cagcccgaag ctcgactacg
     2881 gcgactactc cacgatcatg tcgttcaacg acatgcgcga caacaagatc aagggctact
     2941 tctgctgggg catgaatccg atgcattcca ccccgaacgg caagcacgcc cgccactcga
     3001 tggccaatct cgactggctc gtcgtcgccg actggttcca gacggagacc tcgaccttct
     3061 gggaagctcc ggacatgaag cccgaggacg tcaagaccga ggtctacttc cttcccgccg
     3121 cgctcatcta tgaaaaaatc ggctccatca acaactccgg tcggtggatc cagtggcgtg
     3181 aaaaggccat cgagccgccg ggagaatgcc gttccgactt cgagatgatg atgcttctct
     3241 ggaagaacat cgtcgagctc tacaagaagg aaggcggcgc ctgcccggat cagatcctga
     3301 agacgaactt cgactacacg atcgacggca agcccgatct gcgcgccctc tgctgggccc
     3361 tcaacggcta cacggtcggc gacggcaagc tcctcaaggg ctacggccag cttcaggctg
     3421 acggcacgac cgcttgcggc atgtggatct tctcgggcta ctacaacaac gagtcccaga
     3481 agctcgaccc gatgaagcag ccgatgacaa accgctcgaa ggaagacccg accggtctcg
     3541 gtctcttccc cggctggtcc ttcgcctggc ccgccaaccg ccgcatcctc tacaaccgct
     3601 gctccgccga cccgaagggc aagccctggg atccgaagcg cgtgctcgtc gaatgggacg
     3661 gcaagaagtg gctccagaac gacgtgggcg acttcgtcac ggccaaggcc cccgacgaca
     3721 acgccttctt catgacctgg gagcagaacg cccgtctctt cgcctactcg atggccgacg
     3781 gtccgctgcc cgagcacttc gaaccgcatg aggctccggt gaagaacctc ctcaacggtg
     3841 caggcggcaa cccgtgcgct cgcttcacgg aggacccgtc ggtccgccgc ggtagcatca
     3901 aggactaccc gtacgtggtc acgacctact ccgtcgtcga gcactggcag tccggcacgc
     3961 agacccgcaa catcccgtgg ctcaacgaac tcatcccgag caacttcatc gagctttccg
     4021 aagagcttgc gaaggaaaag ggcattcgtc cgggcgacaa ggtccgagtc tggaacaacc
     4081 gaggctccgt cgtggtcgac gccatgatca ccatccgcat gaagccgatg atgatcgacg
     4141 gcaagctcac gcacgtggtc ggcatgccgc accacttctc ctgggcgacg aagctcgcca
     4201 ccggcgacaa cgtgaacgac ctcactccca acgtcggtga cccgaactcg tacattccgg
     4261 aatacaaggc gttccttgtc aaccttgaga aggcagcctg acggcgtgcc aatggagatg
     4321 aatgatggca aacactaaag aagtcgccat gctgttcgac tcttcgcact gcaccggctg
     4381 caagggctgc caggttgcgt gcaagcagtg gaacatgctt ccctcgaagc tcggcctcaa
     4441 cgagaacaag ttctcgggca cgtatcagaa cccgcctgac ctcaacggcg acacgcgtct
     4501 cgtgatgacg ttcgacgaga aggagtccgg caacaagtac cagccgatca acttcgcaat
     4561 cggccgccgc tcgtgctacc actgcacgga cgccgcctgc gttgcgatct gctcgtccgg
     4621 cgccctctac aagaaggaga acggcgtcgt cgcgtacgac acggacaagt gcaacggctg
     4681 cacgtactgc cagtccgcct gcccgtttga cgtgccccgc ttccgtccga tggacggctg
     4741 cgtggacaag tgcacgctct gcatcgaccg tctcgaagag ggcggcatcc cggcctgcgt
     4801 caagacctgc cagcctgagg cgctcaagtt cggcccgcgc gacgaaatga tcgcgctcgg
     4861 tcagaagcga gtcgagttcc tcaagaagaa gggctttgac aaggccgagc tctacggcgt
     4921 caacgagatg ggcggtctgc acgtgctgca ggtctgccag ttcggtcacg aagcctatgg
     4981 cctcccgacc gatcccaagc ccaacaagat gatcggcatg atgaacgcca tgcgcacggt
     5041 gaccggtgtc ggcaccgctg cggtgctcgc cggcctcgcc gtttccttcg tcgccgccac
     5101 cggctaccgc cgcgaaaagg tgaccgtcga ggaagccaag aagcactgga cgcctgaaca
     5161 gcgcgccacc gccgatcgtg aagtcaagga gcttcttgaa aaagaagcct ccggcaagta
     5221 ataggagact cagaatgacc agatacattc agcgtcactc tctgctcacc cgcgtgactc
     5281 acggcgttgc cgccgtcgcc tgcatcctgc tcgcggtcac cggcgtgttc gtgttcgttc
     5341 cgagcctcgg cggcggcatt atgggcggtg agttcaccca gaccatgcgc atgctccatc
     5401 gcgtcctcgc ggttccgttc atcctcgttc cgctcttcgc cgtgctgaag tcgcccgccg
     5461 gcttcgtgca cctcttcaag gaggacatct tcggcaagtg ggacaaggac gacgtgacct
     5521 gggccatgaa attcccgttc tatctgtttg cgccgggtaa ggttcacatg cccccgcagc
     5581 accacgtgaa gtccgcccag cgtctcgccg acggcgccct gctgttcttc tgcatcatgc
     5641 tcgcgctctc gggcatcgtg ctctggctca acacgggttt cccgaccggc tccggcctca
     5701 aggtcgactt cgcccctgaa acgctgcttc ttgcacacct cgtgcatgac gtgttcttct
     5761 tcctcaccgt ctttgtcggc atcgcccaca tctatctcgg cgccggcatt ttccagccgt
     5821 atcgcggcac cgcccgcatc atgttcggcg acggcaaggt gagcgagtcc gacgcggtct
     5881 accactgggg ctactgggcg aatgaggaaa tcgcccttgg caagaacgtg accgttgaaa
     5941 aggacggcaa ggaaaagtaa ggggaagctt ttccgaactc tccgaaagag ccgccggtgc
     6001 ttctgcgacg ccgacgaccc ccggacgggc cccgcagggg gctcgtccgg aaagaggcct
     6061 ctagagactt ggagacctct ttccggacgc ccgaagaggc gccgaatcca caatcagaaa
     6121 acgcgcattc cgggcttacg gcacggagtg ccttgaaaaa aaggaaagtc acatgctcaa
     6181 ccgcaagtcg atcgaaaggg cccttgcccg tgccgccggt caccaggacg aaagcgtcgt
     6241 cgcgcgtctc gcgctctttg cgcccgcgct gctcaaggcg gcggatctgc gcgcacagct
     6301 tgccgctgaa gctcccgagt ggactgacga agagatcgag gccgcccgtc tgggcaagga
     6361 gacgcttctc tcgctccgtc ccgtcacgat tgactcggag cgcttcctgc agacggtcga
     6421 cgccatcgcg gcggcccttc tcgacaacga cgagatcgag ggcgacatgc tcgaagc
//
LOCUS       DBMDTI010000102         6458 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3064_length_6458_cov_2.678622, whole genome shotgun sequence.
ACCESSION   DBMDTI010000102 DBMDTI010000000
VERSION     DBMDTI010000102.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3064_length_6458_cov_2.678622"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            723..1304
                     /locus_tag="ACZPB6_06035"
     CDS             723..1304
                     /locus_tag="ACZPB6_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HIR1184061.1"
                     /translation="MLEALQTLLLSTGVVTASEIGDKTMLMAICLAARFRRPAPIIWG
                     ILVATVLNHAMAGGVGAVLATLITPEILRWILIVSFAAMAAWMLVPDKLDDEETDEGR
                     MKRFGVFGTTVVLFFLAEMGDKTQLATVALAAKFPAEALWVISGTTLGMMIADAPAVF
                     IGNKLAERLSMKLMRRIAAAVFAILAVAAWLGC"
     gene            complement(1811..2293)
                     /gene="fabZ"
                     /locus_tag="ACZPB6_06040"
     CDS             complement(1811..2293)
                     /gene="fabZ"
                     /locus_tag="ACZPB6_06040"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR1184062.1"
                     /translation="MSTLDIRDIMDMLPHRYPFLLIDRIVELDTEAGRVVALKNVTAN
                     EPQFTGHFPGVPVMPGVLIIEAMAQACAIIALSRLPEGTDRKNSLFYFAGIDKARFKQ
                     VVQPGDQLMIEGKFIRSKLGLGIFEAVARVDGKVVAQAEMMCAYRPVAPKTSDAGKQA
                     "
     gene            complement(2392..3489)
                     /gene="lpxD"
                     /locus_tag="ACZPB6_06045"
     CDS             complement(2392..3489)
                     /gene="lpxD"
                     /locus_tag="ACZPB6_06045"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR1184063.1"
                     /translation="MAENLPRYAVTDLIARVSGMSRGRLEMRLLGECAEVSRFMPPQI
                     AGEGDMAFLTNAAYAEAMKASRASLLIMREQDVEALFGSDEPGRAVLVCDNPYAFFAF
                     ASQILFHVETPAGIHPRAFVEEGAEVDPSATVEALAVVRRGARVGARTVIKTGAVVGE
                     DTVVGEDCILYPNCVLERGTVVGNRCIFQPGCVIGGDGFGFAPFKGEWVKIPQVGRVV
                     IGDDVEIGAGTTVDRGALEDTVIGDGTKLDNQIQLGHNDRIGRHVVMAACVGIAGSTT
                     VGDHCMVGGAAMINGHINIPAGSGIGPGTAITGWGKEPAQKTGFFPALEGRDFQLVGA
                     TISRLPAMRKELKALARRMAELEALIRSAEE"
     gene            complement(3574..4104)
                     /locus_tag="ACZPB6_06050"
     CDS             complement(3574..4104)
                     /locus_tag="ACZPB6_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR1184064.1"
                     /translation="MKKLLPSVLSAAVLGLAMLAPVGAADLRIGVVNMERILRDSLSA
                     AQAGERLNAEGMRRQEEIDALTKRFKQRLERFEKGASDMSESERVTERRELAEMERDV
                     ARRSREARDEFNQRRNEEVLLLQGRAGRIIQQIAETEKFDLVLYEFFYASPKVDITDR
                     VMKALDADVKPAKKKK"
     gene            complement(4229..>6458)
                     /gene="bamA"
                     /locus_tag="ACZPB6_06055"
     CDS             complement(4229..>6458)
                     /gene="bamA"
                     /locus_tag="ACZPB6_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR1184065.1"
                     /translation="MRTEPGTVFSHLPFRTGDEYTPDMGTRAIHSLYRSGLFKDVSLS
                     QDGDVLVVHIVERPAVATIETHGIKAFDKDAVETSLRDVGMAEGRIFDQATLDRADQE
                     LRRQYLARGYYGVKIKTIATPLERNRVRITINVDEGRASSIASIRFTGNRLFDADELL
                     DQMQLGMPNWFSWYTKRDLYSREKLAADLETIRSFYMNQGYLDFKIDSVQVSIAPNKS
                     DVYITINMTEGEKYTIAGAKLTGDLLGIDEELNKLLTLKAGEIYSAEQVNNVSTTLVD
                     KLSTLGYAFATATPNPIADRENRTVEVVYTIDPGRRAYVRRVNITGNNRTKDEVIRRE
                     VRQYESAWFDSSKVKLSRDRIDRLGYFESVTADPVPVPGTRDQVDVDIKVKERPTGSV
                     SLGAGYSTSDGIILSAGFAQNNVFGTGKSVSVDVNTSKSQRTYALSVVEPYITPEGIS
                     RSWEVYDRRVDLEELDVADVEYETRGASLAFGIPFTENDRVFLGGKIESTKVDANSNS
                     PQRYQDYVNRYGADPVSVAATVGWSRDSRDNSLAPTRGVYQRLNGEVGLPGMDIEYYK
                     FTYQYQHYFPLSRTWTLAFNGEVGYGDVYGQTDMFPFFKNFYVGGIGSVRGFESGTLG
                     PKDSTNKMNDDADNLGGDRMVNASLELLAPLPGGDRTLRIFGFLDAGYAWGYEGFVNA
                     NGHADYRRQEFSMSDLRYSTGIGVAWISPLGPLKFSLAFPLNEKEGDKTQRFQFQIGT
                     GF"
BASE COUNT         1125 a         1994 c         2092 g         1247 t
ORIGIN      
        1 tcaagcaaat taagcgacga gcttatggct tccgagacga tctgtacttt ttcttaaaag
       61 tcaaggccgc gttccacgga cttccgagat gaaccaaaaa agaggcccgc gggcaacgcc
      121 tgcaggcctg aagctttcca accaaaagga gcggaggtcc tccgtgctcg tcgcacttcg
      181 ttctccatgt ctcgtacttt agggcatgtc gcttcgcgag ctgtttcgac ttcccggcgg
      241 acgtaacaga gcgcaggacg aaacctcgcc cgcgaaccta gggttaaccc tccagacgct
      301 actgcgagag gtcttttcag ttgcgggcgc aggcgaaagg ctcgcggcga cttttgcatt
      361 ccgtagcatc tcaggcgcct ttcttacggg ttctcggaag gaaaattcct ccgaaaaccc
      421 ctgcggcact gcataacggc atgctagaat gcgctccgca ctcttcaggg gagtagctcg
      481 ggcggccgca acggccgccg tcctgcgtca acatgcttga agcgccttgc tttatggcgc
      541 aggaatgcga gcctcgggga ctcatcgttc ccggcgcggc atgagcaaga cctgcggtgt
      601 gtcctcttcc ggataaaccg caaggacgcc caggacgcgc ccgggccggc gcatcgggca
      661 ggaagaggat gcgcccgcag tttcttaaca catgccccac gcgccgtccc cgacaatccc
      721 ccatgcttga agcccttcag actcttcttc tctccaccgg cgtcgtcacc gcaagcgaaa
      781 tcggcgacaa gaccatgctc atggccatct gcctcgccgc cagattccgc aggcctgctc
      841 ccatcatctg gggcatcctc gtcgccacgg tcctcaacca cgcgatggcg ggcggcgtgg
      901 gcgccgtgct cgccaccctc atcacgcccg aaatcctccg ctggatcctt atcgtctcgt
      961 tcgccgccat ggccgcctgg atgctcgtcc ccgacaagct cgacgacgag gagacggacg
     1021 agggccgcat gaagcgcttc ggcgtcttcg gcaccaccgt cgtgctcttc ttcctcgccg
     1081 aaatgggcga caagacccag ctcgccaccg tggcgctcgc cgcgaagttc cccgccgagg
     1141 ccctctgggt catctcgggc acgacgctcg gcatgatgat cgccgacgcg cccgccgtct
     1201 tcatcggcaa caagctcgcc gagcgcctct ctatgaagct catgcgccgg atcgccgccg
     1261 cggtcttcgc catcctcgcc gttgccgcct ggctcggctg ctgatcctgc cgcactcacc
     1321 ccgccgccac ggcggcaggg gcctgaagcc tcgaaaaaaa taaaaaaaag cccgcggtct
     1381 cagtgacagc gggcgaataa atccaaccaa aaggagcgga ggactcgtcg ccttccggcg
     1441 tcgttctccg atgagctaac tttacctgac gagctctccg ccaaggtttc agacgtcggg
     1501 gcaaatgtaa caagtcttca ggaaaaaccc taggttgatt agtatttact ctgtctccct
     1561 cccgctcttg cccgtcacca caggcgaatt caaacttcac aagaaaggcc taggcagttt
     1621 gtactagatg cattttgttg catttctagg ggtttcttcg tcccttcagg tgcctttcga
     1681 gccctttggc aaggcttttt tccctgttcg cgcttcccaa aaaaatcctg cgcggagagg
     1741 attctcctgc gcaggacttc tgctgccgcc ggctttgaca agcttcaggc gcgtcgggac
     1801 ttcaggggcc ttaggcctgc ttgccggcgt cggacgtctt gggcgcgacg gggcggtagg
     1861 cgcacatcat ctcagcctgg gcgacgacct tgccgtcgac gcgggcgacg gcctcgaaga
     1921 tgccgagacc gagcttggag cggatgaact tgccttcgat catgagctga tcgccgggct
     1981 gcacgacctg cttgaagcgg gccttgtcga tgcctgcgaa gtagaagagc gagttcttgc
     2041 ggtccgtgcc ttcgggaagg cgggagagcg cgatgatcgc gcaggcctgg gccatcgcct
     2101 cgatgatcag aacgccgggc atgacgggca cgccggggaa gtgtcccgtg aactgcggct
     2161 cgttggcagt gacgttcttg agcgcaacca cgcggccggc ctccgtgtcg agctcgacga
     2221 tgcggtcgat gagaaggaac ggataacggt gcggaagcat gtccatgatg tcacggatgt
     2281 cgagcgtact catattggaa cgtaacctct attgaaaaat ggggccttgg cgtgcggcgg
     2341 gatgccgccg ctctccgggg gcatcctctt attgtcgcgc cttttgaaaa attattcttc
     2401 ggcagaacgg attaacgcct cgagttcagc catgcggcgg gcaagggcct tgagctcctt
     2461 gcgcatggca ggaagacgcg agatcgtggc gccgacgagc tggaagtcgc ggccctccaa
     2521 ggcggggaag aagccggtct tctgggcggg ttccttgccc cagcccgtga tggccgtgcc
     2581 cggaccgatg ccggaacctg cggggatgtt gatgtggccg ttaatcatgg ccgcgccgcc
     2641 caccatgcag tggtcaccca cggtggtcga acccgcgatg cccacgcagg cagccatcac
     2701 gacgtggcgg ccgatgcggt cgttgtggcc gagctggatc tggttgtcaa gcttggtgcc
     2761 gtcgccgatc acggtgtcct cgagggcgcc gcggtcgacc gtcgtgcccg cgccgatctc
     2821 gacgtcgtcg ccgatcacca cgcggcccac ctgcgggatc ttgacccact cgcccttgaa
     2881 gggcgcaaag ccgaagccgt cgccgccgat cacgcagccg ggctggaaga tgcagcggtt
     2941 gccgacgacc gtgccgcgtt caagcacgca gttcgggtaa agaatgcagt cctcgcccac
     3001 gaccgtgtcc tcacccacga cggcgcccgt cttgatgacg gtgcgtgcgc caacgcgcgc
     3061 gccgcggcgc acgacggcga gcgcctcgac cgttgcgctc ggatccacct cggcgccctc
     3121 ttcaacgaag gcgcgcggat gaatgcccgc aggcgtttcg acgtggaaga gaatctggga
     3181 ggcgaatgca aagaaggcgt acggattgtc gcagacgagc acggcgcggc cgggttcgtc
     3241 cgaaccgaag agcgcctcga catcctgctc gcgcatgatg aggagcgagg ccctcgaagc
     3301 cttcatcgcc tcggcgtagg cggcgttcgt gagaaaggcc atgtcgcctt cgcccgcaat
     3361 ctggggcggc atgaagcggg agacttcggc gcattcgccc agaaggcgca tttcaagcct
     3421 tccgcggctc attccggaca cgcgggcaat cagatcggta acggcataac gcggcagatt
     3481 ttcggccatg acggctcctc agtatgttgt ggtttcgttg ttgcatgacg gcgaacgggc
     3541 ctcaggcccg ttcatgccgc tttgcttttt ccattatttt ttcttcttcg caggcttgac
     3601 gtcggcgtca agcgccttca tcacgcggtc cgtgatgtcg accttgggac tggcatagaa
     3661 gaactcgtag agcaccagat cgaacttctc ggtctcggcg atctgctgga tgatgcgtcc
     3721 tgcgcgtccc tgcagcagga gcacctcctc gttgcgtcgc tgattgaact cgtcgcgggc
     3781 ctcgcggctg cggcgcgcaa cgtcgcgctc catctcggcg agctccctgc gctcggtgac
     3841 gcgctcgctc tcgctcatgt cggaggcgcc cttctcgaag cgctcgagac gctgcttgaa
     3901 gcgcttggtg agcgcatcaa tctcctcctg acggcgcatg ccctcggcat tgaggcgctc
     3961 gcccgcttgg gcggcggaga gagagtcgcg cagaatgcgc tccatgttga cgacgccgat
     4021 gcgaagatcc gcggcgccca cgggcgcgag catggcgagg ccgagcacgg cggccgagag
     4081 aacggagggc agaagctttt tcacggtgtt cacctttggg cttgaaaaaa acggggcgcc
     4141 cgatttgggc agccccgtct gcaggcattt tcgtatttta ccgccgcgca cggcccgacg
     4201 acgggccgcc cgcgcggctc ggcaggtctt agaagcccgt gccgatctgg aactggaagc
     4261 gctgcgtctt gtcgccttcc ttctcgttga gcgggaaggc cagcgagaac ttcagcgggc
     4321 cgagcggaga gatccaggcc acgccgatac cggtcgagta gcgcaggtcg ctcatgctga
     4381 attcctgacg cctgtagtcg gcatggccgt tggcgttgac gaagccttcg tagccccagg
     4441 cgtagccggc gtcgaggaag ccgaagatac gcagcgtgcg gtcgccgccc ggcagcgggg
     4501 cgaggagttc aagcgaggca ttgaccatgc ggtcgccgcc cagattgtcc gcgtcgtcgt
     4561 tcatcttgtt cgtgctgtcc ttcggaccga gcgtgccgga ttcgaagccg cgcaccgagc
     4621 cgatgccgcc cacgtagaag ttcttgaaga acgggaacat gtcggtctgg ccgtagacgt
     4681 cgccgtagcc cacttcgccg ttgaaggcca gcgtccacgt cctcgagagc gggaagtagt
     4741 gctggtactg gtaggtgaac ttgtagtact cgatgtccat gcccggcagg ccgacttcac
     4801 cgttgagtcg ctggtacacg ccgcgcgtcg gagcgagcga gttgtcgcgg gagtcgcggg
     4861 accagccgac cgtggcggcc acggacacgg gatcggcgcc gtagcggttc acgtagtcct
     4921 gatagcgctg cggggagttg ctgttggcgt caaccttcgt ggattcgatc ttgccgccga
     4981 ggaacacgcg gtcgttttcc gtgaacggga tgccgaacgc gagcgaggcg ccgcgggttt
     5041 cgtattccac gtcggcaacg tcgagctctt caaggtcgac gcggcggtca tagacttccc
     5101 acgagcggct gatgccctcg ggcgtgatgt acggttcgac gacggagagc gcgtaggtgc
     5161 gctgggactt cgacgtgttg acgtcgacgg acacggactt gccggtaccg aagacgttgt
     5221 tctgcgcgaa gcccgccgaa aggatgatgc cgtcggacgt ggagtagccc gcaccgagcg
     5281 agacggagcc ggtcggacgc tccttcacct tgatgtcgac gtcgacctgg tcgcgcgtgc
     5341 cgggcacggg caccgggtcg gccgtcacgg actcgaagta gccgagacgg tcgatgcggt
     5401 cacgcgagag cttgaccttg gagctgtcga accaagcgga ttcgtactgg cggacttcac
     5461 ggcggatcac ctcgtccttc gtgcggttgt tgcccgtgat gttcacgcgg cgaacatagg
     5521 cgcggcggcc cgggtcgatc gtgtagacga cctcgaccgt gcggttctcg cgatcggcga
     5581 tcggattggg cgtcgcggtc gcaaaggcgt agccgagcgt ggagagcttg tcgacgagcg
     5641 tcgtggagac gttgttgacc tgctcggcac tgtagatctc gcccgccttg agcgtcagga
     5701 gcttgttgag ctcctcgtcg atgccgagaa ggtcgcccgt gagcttggcg ccggcgatcg
     5761 tgtacttctc gccctcggtc atgttgatcg tgatgtagac gtcggacttg ttgggcgcga
     5821 tggaaacctg gacggagtcg atcttgaagt cgaggtagcc ctgattcatg tagaaggagc
     5881 ggatcgtctc aaggtcggcc gcaagctttt cacggctgta gaggtcgcgc ttcgtgtacc
     5941 agctgaacca gttcggcatg ccgagctgca tctggtcgag cagctcgtcg gcatcgaaga
     6001 ggcggttgcc cgtgaagcgg atcgaggcga tcgacgaggc acggccttcg tcgacgttga
     6061 tcgtgatgcg cacgcgattg cgttcgagcg gcgtggcgat cgtcttgatc ttcacgccgt
     6121 aatagccgcg ggcgaggtac tggcggcgca gctcctggtc ggcgcggtca agcgtcgcct
     6181 ggtcgaagat gcggccctcg gccatgccga cgtcgcgcag gctggtctcg acggcgtcct
     6241 tgtcgaaggc cttgatgccg tgcgtctcaa tggtcgcgac ggcgggacgc tcgacgatgt
     6301 gcacgacgag cacgtcgccg tcctgggaga gcgacacgtc cttgaagagg ccggagcggt
     6361 agagcgagtg gatggcgcgg gtgcccatgt cgggcgtgta ctcgtcgcct gtgcggaacg
     6421 gaaggtgcga aaagaccgta ccgggttcgg tacgcatc
//
LOCUS       DBMDTI010000103         6411 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3083_length_6411_cov_2.803645, whole genome shotgun sequence.
ACCESSION   DBMDTI010000103 DBMDTI010000000
VERSION     DBMDTI010000103.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3083_length_6411_cov_2.803645"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /gene="csm6"
                     /locus_tag="ACZPB6_06060"
     CDS             complement(<1..929)
                     /gene="csm6"
                     /locus_tag="ACZPB6_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated ring nuclease Csm6"
                     /protein_id="HIR1184066.1"
                     /translation="MTGMTPQVITETIYALKTERDIVPDEVHVVTTSSGADCIRETLL
                     DPSCGRWQDFVSALGADKTISFNAANVHVIRTADGRKLDDIRTPEDNTWAADCITECV
                     RSFCARNDVRLLVSMAGGRKSMGFYAGYALSLYGRAFDGLYHVLVSPPYENLPDFFYP
                     AQQSPECLNIRNVRLAACDAEIMLADIPFVRMGNDPASNRGSYMSAVREAQNHQPMPA
                     ALMFDESRRTVVAGGVTIKFPPLLFAVYLWLSLRAKDGKPPCRPGKDVFAEEFLGIYR
                     RVSGGETPDYEHACSALSRDEDFLPYFQEKRAL"
     gene            complement(956..2116)
                     /locus_tag="ACZPB6_06065"
     CDS             complement(956..2116)
                     /locus_tag="ACZPB6_06065"
                     /inference="COORDINATES: protein motif:HMM:NF048543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Card1-like endonuclease domain-containing
                     protein"
                     /protein_id="HIR1184067.1"
                     /translation="MTNEDVQDPLNEVGVHFCLVSKQAAVNLLPVEHYRPNAVVLFVS
                     HEMQAQARDLQAAMLKSLPGLRINIISLADAWDLAGVRDAVWGQLEGLCCDVNQIAVN
                     VTGGTKIMMLGALLAASAAGARCFYLNESDNSISLIDPDSNDGSRRMQKVRLQTKVET
                     YLKAYGFQVEDSSRMPSVSMPEKMLIESLINTPSFRDVMPLLNMLSTDRETEKALKVS
                     FKGRIPPQNRAAFDALCDRFIQTGHLNIRGDIMTFPSEADRFFVAGGWLERYVFNTLS
                     DMKMKPEGNITVIGDAKNEIDVAFFHEGSLCIVECKTGNLSASEDANQVIYKLESLKK
                     LGGIKTKLILVSYKPISAKSNIRATGDKTHILLIQGDQLKRLKDNLTRCLQK"
     gene            2145..2546
                     /gene="cas2"
                     /locus_tag="ACZPB6_06070"
     CDS             2145..2546
                     /gene="cas2"
                     /locus_tag="ACZPB6_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HIR1184068.1"
                     /translation="MSMITQNHTSMGSHKFVIFHRLGDLSDNYGAEGCEKQISLMSQG
                     RKLYLVLYDIVDAPRQSRVRGFISAYAISHQKSFYECWLTASELAELIGHVGNALTDA
                     DRFHCFELDPRMLNRLTGTARRISTEPFLVV"
     gene            2553..3509
                     /gene="cas1"
                     /locus_tag="ACZPB6_06075"
     CDS             2553..3509
                     /gene="cas1"
                     /locus_tag="ACZPB6_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HIR1184069.1"
                     /translation="MSTLYIDRKGLGCKLENGALVFYENGERCGTVPTAPLERIVFKS
                     DVSINTGALAKLAARGIGMLFLSGHAIKPTLFLPTEHKDAERRRSQYQLSEDPEFCRL
                     FAVDLLTEKLQAQKRHVVDLSMGNHDEKAFLESSITRLDELLGRLNAEPSLDALRGLE
                     GLAAAVYFEALGRCVSPELGFTHRNRRPPRDPVNALLSLTYTLLTSEAGVSAHQAGLD
                     PFVGFLHSLEYGRPSLACDLMEPLRPEADRFVLSLFQTKTITLSDFTKTTERSLLSKD
                     GRIKFYPQYEAVAGEWRRYLNKRSLKHASTFVTQHRSHKQSQ"
     gene            3514..3807
                     /gene="csx16"
                     /locus_tag="ACZPB6_06080"
     CDS             3514..3807
                     /gene="csx16"
                     /locus_tag="ACZPB6_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx16"
                     /protein_id="HIR1184070.1"
                     /translation="MAVIFVSRHAGAIEWMKRQPVHVDRWVSHLQASEVNAGDTVIGV
                     LPMHLAADICAKNARYLALEFNMPMAFRGQELTCEELDGIHCRMCEYVVHRRT"
     gene            3828..4112
                     /gene="cas2"
                     /locus_tag="ACZPB6_06085"
     CDS             3828..4112
                     /gene="cas2"
                     /locus_tag="ACZPB6_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HIR1184071.1"
                     /translation="MGLYLVAYDIRDRNRWHKVHHMLIAQALPIQYSVFLFNGSRDAF
                     ERCMSALTSIIDPREDDLRFYELPKRGLKYRAGKPQLPTGIYFSGTPSEL"
     gene            4391..5533
                     /locus_tag="ACZPB6_06090"
     CDS             4391..5533
                     /locus_tag="ACZPB6_06090"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1184072.1"
                     /translation="MPLNRRNFLGAAALGAGSLMLGSAQAAPAPALPKFTPPTAKKPM
                     ALCFNENPLGLTPAARDAAIAALPDANRYPFVAAERLRKACADFMGGKPENVMLSHGS
                     AESIRASIEAHIRPGVCLICPELTYSDGADTARKNNVKVVTVPMGAKWSVDLEAMKKT
                     VAGINGPVIVYFVNPNNPTSTIVDSKALHDWIRSRPADVFFVIDEAYAEYVRDPSFVS
                     CKTLIDEGLENLCVLKTFSKIFAMAGMRLGFTYAAPAVVNKVRTQVAYDIMLNNSAIA
                     AALVELEDKDFIPTSRKANEDARVIVCKTLDELGIEYLDSQTNFIFMNLKAPLKPFQD
                     RMKAENILVGRPFPPAVEWCRVSLSTPDDMKYFAAKLKEFRRKGWV"
     gene            complement(5660..>6411)
                     /locus_tag="ACZPB6_06095"
     CDS             complement(5660..>6411)
                     /locus_tag="ACZPB6_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1184073.1"
                     /translation="EDGKAFYRVAKESMRAWHMLEDFPKQRASTKKQLRIAVLARHCS
                     NVIIPAVAKILKAHEETLRITMDVHQSRDIYYSKFSHPFDVGFGTLLSSHDDLQKEAI
                     AHLPFRLVVPKTNPMSRMEKVRLEDYAHENFILLSADKLEREYIRSLHPALTPDRIVA
                     ELSSTQVALRFVKRGIGVHITDELAAMSVSDDCAAVPLENPLTIPFYVFWPVTSESLW
                     PEIRQCIAEIAGSIRAAGIPLTEAGESYLKA"
BASE COUNT         1408 a         1950 c         1678 g         1375 t
ORIGIN      
        1 atcaacgccc gcttttcttg aaaataagga aggaaatcct cgtctcgcga cagagcgctg
       61 caggcatgct catagtcggg cgtctcacct ccagatacgc ggcggtatat cccaagaaac
      121 tcctcagcaa aaacatcctt tccgggacga cagggcggct tgccatcttt tgcccgcaag
      181 gagagccaga gatagacggc aaaaaggaga ggcgggaact taatcgtcac tcctccggcg
      241 acgacggttc gtctggactc atcgaacatg agtgctgcgg gcatcggctg atggttctgc
      301 gcttccctga cggccgacat atagcttcct cggttcgacg caggatcgtt tcccatgcga
      361 acgaatggta tgtcggccag catgatctcc gcgtcgcacg cggccagccg cacattgcga
      421 atgttcagac attccggcga ctgttgagca ggatagaaga agtcgggcag gttttcatat
      481 ggtggcgaaa ccagtacatg atagagtccg tcgaatgctc gcccataaag cgacaacgca
      541 tatccggcgt aaaaccccat gctttttcgc cctcccgcca ttgagacgag cagacgaaca
      601 tcattgcggg cacagaaact tcgcacgcat tccgtgatgc agtccgccgc ccaagtattg
      661 tcttccggcg ttcgaatgtc gtcgagtttt cgaccgtcgg cagttcgaat cacatgcaca
      721 ttcgccgcat tgaaagagat ggtcttgtcg gcccccagcg cactgacaaa atcctgccaa
      781 cgaccgcatg agggatcgag aagcgtctca cgaatgcagt cggcaccgga acttgttgta
      841 acaacatgca cctcatccgg cacaatgtct cgttcagttt tgagggcata gatcgtttcc
      901 gtgatgacct gaggagtcat gcccgtcacg gcgagcaaaa tgcatcgcct cgagttcatt
      961 tctgcaggca tcgcgtcaaa ttgtccttca agcgcttgag ctggtcaccc tggatgagaa
     1021 ggatatgcgt cttgtctccg gttgctcgaa tgttggattt ggcactgatc ggcttatagc
     1081 tcaccagaat cagcttcgtc ttaatccccc ccagtttttt gagtgactca agcttgtaga
     1141 tcacctgatt ggcatcctca ctcgcgctca agttcccggt cttgcattcg acgatgcaga
     1201 gcgaaccttc atggaagaaa gcaacatcga tttcattctt cgcgtccccg atgaccgtga
     1261 tattgccttc gggcttcatc ttcatgtcgc tcagtgtatt gaaaacatag cgctccagcc
     1321 agcctccggc aacgaagaag cggtctgctt cggaaggaaa cgtcatgatg tcgccgcgaa
     1381 tgttcaggtg tcccgtttgt ataaaacggt cgcagagtgc atcaaacgct gctcggttct
     1441 gaggcggaat gcgccccttg aaggaaacct tgagcgcctt ctccgtttcc cggtcagtcg
     1501 acagcatgtt gagaagcggc atgacgtctc gaaaagaggg cgtattgatc aggctttcga
     1561 tcagcatttt ttccggcatg ctgacggacg gcatccgact gctgtcctcc acttggaatc
     1621 cgtaggcttt cagataagtt tcgacctttg tttgcaaacg gactttctgc atgcggcgac
     1681 tgccatcgtt actgtcagga tcgatcagac tgatcgaatt gtcagattcg ttgagataga
     1741 agcaacgcgc acctgctgcc gaagccgcca gaagcgcacc gagcatcatg atttttgttc
     1801 cgccggtcac attgacggcg atctgattga cgtcgcagca caacccctca agctgacccc
     1861 aaacggcatc acgaacaccc gccaaatccc aggcgtctgc aagcgaaatg atgttgatcc
     1921 tgagccccgg gagagattta agcatggccg cctgaagatc acgggcctga gcctgcattt
     1981 catggctcac aaacaagacc actgcattcg gacgatagtg ctccaccggg agcagattga
     2041 cggctgcctg cttcgaaacc aagcagaaat gaacaccaac ttcgttcaaa ggatcctgaa
     2101 catcttcatt agtcatggcg cactccttca cgtgaatcta acccatgtcg atgattactc
     2161 agaatcacac ctcaatgggg agtcacaaat ttgtcatttt tcaccgcctt ggtgacctat
     2221 ctgataatta tggagcagaa ggttgtgaaa aacaaatttc gcttatgtct caaggaagaa
     2281 aactctatct cgtgctatac gacatagttg acgcccccag gcagtcacgc gtaagaggct
     2341 tcatatccgc ttatgccatc agccatcaga aatcctttta cgaatgctgg ctgacggctt
     2401 cggaacttgc agaactgatc ggccatgtcg gcaatgcatt gactgatgcc gatcgattcc
     2461 attgctttga gcttgatcca cgcatgctga accgcctgac agggactgct cgtcgaatct
     2521 ctacggaacc ttttcttgtt gtttgaatat ccatgtctac gctgtacatt gatcgcaagg
     2581 gactcgggtg caagttggaa aacggtgcgc ttgttttcta tgaaaacgga gagcgatgcg
     2641 gaactgttcc gaccgctccg cttgaacgga tcgttttcaa aagcgatgtc tcaatcaata
     2701 cgggagcact ggccaaactc gccgctcgcg ggatcggcat gcttttcctt tccgggcacg
     2761 caatcaagcc gactcttttt ctgccgacgg agcacaagga cgctgaaaga aggcgcagtc
     2821 aatatcagct gagcgaggat cctgaattct gccgcctgtt tgcagtggac cttttgacgg
     2881 agaaacttca ggcacaaaag cggcatgttg tcgatctttc aatgggaaac cacgacgaga
     2941 aggcgttttt ggaatcttcc ataacgcggc tggacgaact tttagggcgt ctgaacgctg
     3001 agccttcgct cgatgcatta cgcgggcttg aaggtctggc cgcagccgtg tattttgaag
     3061 cgctgggtcg ctgcgtcagt ccggagcttg gatttaccca tcgaaaccga cgtccgccca
     3121 gggatcctgt caatgcactg ctgtcgctga cctacacctt gcttacatca gaagcaggcg
     3181 tgtcagccca tcaagcaggg ctcgatccgt ttgtcggatt cctgcacagt ttggaatacg
     3241 gacgtccttc gcttgcctgc gacttgatgg agcccctgcg tccggaagca gaccggttcg
     3301 tacttagttt atttcaaaca aaaaccataa cattatcgga cttcacaaaa acaacagaac
     3361 gcagcctgct ctccaaggat ggacgcatca agttttatcc tcagtatgaa gcagttgccg
     3421 gtgaatggcg gcgctacctg aacaaacgca gcctcaagca cgcatcgacc tttgtcaccc
     3481 aacatcgttc acacaaacag tcgcaataaa gtcatggccg tcatttttgt ttcacgacac
     3541 gccggagcaa ttgaatggat gaagcgtcag cccgttcatg tcgatcgctg ggtctctcat
     3601 cttcaggcca gcgaagtgaa tgcaggagac acggtcatcg gtgtgctgcc catgcacctt
     3661 gccgcggaca tctgcgccaa gaacgcccga taccttgcac tggagttcaa catgccgatg
     3721 gcatttcggg gacaggaact gacgtgcgaa gagctcgacg gcatccactg ccgaatgtgt
     3781 gaatacgtcg tgcaccgacg aacctaagat ttgaggagat cccgttcatg gggctctatc
     3841 ttgtagctta cgacattcgc gatcgcaacc gatggcacaa ggttcaccac atgctcattg
     3901 cccaggcgct gccgatccaa tacagcgttt tccttttcaa tggttcccgg gatgcgttcg
     3961 aacgctgcat gtcagccctg acatccatca tcgatcctcg ggaagatgat ctgaggtttt
     4021 acgaactgcc aaaacgcggg ctgaagtatc gtgctggaaa gcctcaactg ccaacaggga
     4081 tctatttttc gggaacaccg tcagagctgt aactttacaa catgcccagt gtctcaacta
     4141 ggcacgcttc agcctccaat ttcaggcagg agcagtcaca ccaagatact gcacggcagt
     4201 gttcacgaga accgttctaa tccgttttcc ctaaagcatt ccgcccaagc gctctgaagt
     4261 cgctcaacaa agtgcgaaat tccctcttgc agagcgcatc aaggagacat caagagcctt
     4321 cgttcgagtc acaatgactg aaacgccggt gccgcgcacc ggccccagtc acagacaccc
     4381 ggagagcatc atgcccctga accgccgcaa tttcctcgga gccgccgccc ttggcgcggg
     4441 ctccctcatg ctcggctccg cgcaggccgc gcctgcgccc gccctgccca agttcacgcc
     4501 gcccacggcc aaaaagccga tggcgctctg cttcaacgaa aatccgctcg gcctcacgcc
     4561 cgccgcccgc gacgccgcga tcgcagccct gcccgacgcc aaccgctacc cgttcgtcgc
     4621 agctgaaagg ctccgcaagg cctgcgccga cttcatgggc ggcaagcccg agaacgtgat
     4681 gctctcgcac ggctccgccg aatcgatccg cgcgagcatc gaggcccaca tccgtcccgg
     4741 cgtctgcctc atctgtcccg aactcaccta cagcgacggc gccgacacgg cccgcaagaa
     4801 caacgtcaag gtcgtcaccg tgccgatggg cgcgaagtgg tccgtcgacc ttgaggccat
     4861 gaagaagacc gtcgcgggca tcaacggccc cgtcatcgtc tacttcgtca acccgaacaa
     4921 cccaacctcg accatcgtcg actcgaaggc ccttcacgac tggatccgct ctcgtccggc
     4981 cgacgtcttc ttcgtcatcg acgaggccta cgccgaatac gtccgcgatc cgtccttcgt
     5041 ctcctgcaag acgctcatcg acgaggggct cgaaaacctg tgcgtgctca agaccttctc
     5101 gaagatcttc gccatggccg gcatgcgcct cggcttcacc tatgccgcgc ccgccgtcgt
     5161 caacaaggtc cgcacccagg tcgcctacga catcatgctc aacaactcgg ccatcgccgc
     5221 cgcgctcgtc gagctcgaag acaaggactt catcccgacc tcccgcaagg ccaacgagga
     5281 cgcccgcgtg atcgtctgca agaccctcga cgaactcggc atcgagtacc tcgacagcca
     5341 gacgaacttc atcttcatga acctcaaggc cccgctcaag cccttccagg accgcatgaa
     5401 ggcggagaac atcctcgtcg gccgcccgtt tccgcccgcc gtcgaatggt gccgcgtctc
     5461 gctctcgaca cccgacgaca tgaagtactt cgccgcgaag ctcaaggagt tccgccggaa
     5521 gggctgggtc tgatccgatg agccttccgc cgcgggacga ccgtcccgcg acgccttgaa
     5581 aaaaatcccg cgcgccgctg ccggccgtgg gatttttcct ttccgcttcc cttcggccgc
     5641 cgttcgaccc aggacctcat caggccttca gatagctctc gcccgcctcc gtgagcggga
     5701 tgcccgccgc gcggatgctg cccgcgatct cggcgatgca ctgcctgatt tcgggccaga
     5761 ggctctccga ggtgacaggc cagaagacgt agaagggaat cgtgagcggg ttctcgaggg
     5821 ggaccgccgc gcagtcgtcc gagacgctca tcgccgcgag ctcgtccgtg atgtgcacgc
     5881 cgatgccgcg cttgacgaac ctcagcgcca cctgcgtcga ggagagttca gccacgatcc
     5941 tgtcgggcgt gagcgcggga tgcaggctcc tgatgtactc gcgctcaagc ttgtcggcgg
     6001 aaagcaggat gaagttctcg tgcgcatagt cctcgagcct caccttctcc atccggctca
     6061 tgggattggt cttcggaacc accagcctga agggcaggtg cgcgatcgcc tccttctgca
     6121 ggtcgtcgtg actgctgagc agcgtgccga agcccacgtc gaagggatgc gagaacttag
     6181 agtagtagat gtcgcggctc tggtgcacgt ccatcgtgat cctcagcgtc tcctcgtggg
     6241 ccttgaggat cttcgccact gcgggaatga tcacgttgct gcagtggcgg gcgagcacgg
     6301 caatgcgcag ctgcttcttc gtcgaggccc tctgcttcgg aaagtcctcg agcatgtgcc
     6361 aggcgcgcat cgactccttc gccacgcgat agaacgcctt gccgtcctcc g
//
LOCUS       DBMDTI010000104         6382 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3100_length_6382_cov_4.058589, whole genome shotgun sequence.
ACCESSION   DBMDTI010000104 DBMDTI010000000
VERSION     DBMDTI010000104.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3100_length_6382_cov_4.058589"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..101)
                     /locus_tag="ACZPB6_06100"
     CDS             complement(<1..101)
                     /locus_tag="ACZPB6_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1184074.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITT"
     gene            complement(174..248)
                     /locus_tag="ACZPB6_06105"
     tRNA            complement(174..248)
                     /locus_tag="ACZPB6_06105"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(214..216),aa:Thr,seq:ggt)
     gene            complement(353..438)
                     /locus_tag="ACZPB6_06110"
     tRNA            complement(353..438)
                     /locus_tag="ACZPB6_06110"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(402..404),aa:Tyr,seq:gta)
     gene            609..2075
                     /gene="cls"
                     /locus_tag="ACZPB6_06115"
     CDS             609..2075
                     /gene="cls"
                     /locus_tag="ACZPB6_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR1184075.1"
                     /translation="MPSLDSIITFSTLAALVQFGIIISVILRVMLTRHPPGSAFAWIL
                     ITTLLPYFGFILYLLFGERPIGQLRATRFRKHLAHLKELSRQKLTPTGPLPRHLARHR
                     TLIHLATRMGNLPVTSGTEIRLLSSSDDTFAAIISDIEAACSSIDMEFYIWHPGGEAD
                     KVAASLLKAHERGVRVRILVDDFGSRAFLKSDTCRAFRNAGIEVAKAMPMRLLHFFGL
                     QRVDLRLHRKTIIIDNEIGYTGSLNMIDPHTYDAARTVGPWVDAMVRLTGPAVQSLRA
                     TFLIDWAIQPDGDPSDLERVHSVPPIPQLGTATIVTVPSGPYGQKDPNLYLLLDTINA
                     ARFNITITTPYFVPNEGLATALLSAAYRGVKIRLIVPANADSKAVTWAARRYFDDMLS
                     AGVEVLLYNGGLLHTKSISVDDEYAVFGTVNFDNRSIHLNFEMMLLIFDAGFIHDLKK
                     LHDSYAEQCSRLDPETWYRRPVSERLKEGACYLISPLL"
     gene            complement(2082..2450)
                     /locus_tag="ACZPB6_06120"
     CDS             complement(2082..2450)
                     /locus_tag="ACZPB6_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184076.1"
                     /translation="MPLYVFQTSGNPFVRILIGLVSFAVLVGLGFLMLPVVLAVIAAV
                     MVMGLVAWGWAWWKARQAGGTTDWYEAMSRAAASRQASEQGAREDVRETRTETTVLSI
                     KTSDNKKWKMNDVEDIEERK"
     gene            2839..3633
                     /gene="folE2"
                     /locus_tag="ACZPB6_06125"
     CDS             2839..3633
                     /gene="folE2"
                     /locus_tag="ACZPB6_06125"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HIR1184077.1"
                     /translation="MDADNKNTLPDVQSARDGRNIAIDRVGVRGITVPITVETKEDTL
                     ATVATVEMTVSLPADKKGTHMSRFVALMEEHSEPLNVDVVRSLMADMLKRLDAVDGTI
                     ELRFPFFIRKCAPVSKLTSLMNYEAAWIADSRDGVITVRQETATPVTSLCPCSKEISR
                     YGAHNQRSRLTASVELSAPMSLEEQIAYSEEAASCQLWARLKRSDEKYVTEYAYDHPK
                     FVEDIVRDMAAALNKEERVVAYRVEAENFESIHNHSAYAYITHDKR"
     gene            3792..5534
                     /gene="argS"
                     /locus_tag="ACZPB6_06130"
     CDS             3792..5534
                     /gene="argS"
                     /locus_tag="ACZPB6_06130"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR1184078.1"
                     /translation="MLAQQKEALTSLISAALETLGVTGVTVILERPKMPEHGDVACTV
                     AMQLARALKKNPREIATQIVEALKANPESEALIKSVEIAGPGFINLRIQDNARQEIIR
                     QVLKAGTSFGCGDAHKNQSVLLEFVSANPTGPLHLGHARQGALGDVLANLLATQGWKV
                     TREFYYNDAGVQIGNLAESIRLRARELKGEAVELPENAYHGEYVIAIARDYLDCKPVH
                     AHDGTVIESKGDLSDTDCIRRYGVAYLRNEQDDDLDALGVHFDNFYLESSLYASGRVQ
                     AAVQSMIDSGRTEERDGALWLKTTECEDLGIHDDKNRVMRKVDGTFTYFVPDVAYHLA
                     KFERGFTKAVNIQGSDHHGTIARVRCGLQCCALARQMSIPKEFPEYILHKMLSVVKDG
                     EPVKMSKRSGNYVTLRDLVDMAGQDAARFFLVARKADAEFVFDINLAQQKSDENPVYY
                     LQYAHARICSVFANAKEKGYEVPSADELIKADLSSLVSEQAMDLMAKIAEYPSLLTLA
                     AKDHAPHMLCYYLKELAAAFHTFYNAERVVIENDVERNARLALLAATRQVLGNGLALL
                     GISAPEYMARREDA"
     gene            5553..6083
                     /locus_tag="ACZPB6_06135"
     CDS             5553..6083
                     /locus_tag="ACZPB6_06135"
                     /inference="COORDINATES: protein motif:HMM:NF016895.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR1184079.1"
                     /translation="MARTNLASGFAGFLIGVVTGCAICAAAAVFITNSPIPFVEKVQK
                     VTADVDPAQKLAGGIDPNRALNEQQEKSAEQDDSSGVKTVTVAPPVQSQPQSQTPAAG
                     AGGSYWLQAGAFKEEKSAHDLTGSLALTAGISNVQVVKAGSVWRVRIGPFDNKLEASE
                     VQSQLDNQGIQTTVVR"
     gene            complement(6160..>6382)
                     /locus_tag="ACZPB6_06140"
     CDS             complement(6160..>6382)
                     /locus_tag="ACZPB6_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HIR1184080.1"
                     /translation="RDRSLYEALRQWRSDEAKSLGKPPYVIFKDATLLEIVRERPTSP
                     EALRRISGVGEKKLVNYGDAVLDIVAAES"
BASE COUNT         1320 a         2237 c         1620 g         1205 t
ORIGIN      
        1 gtggtgatag cagccgtcag cgtggtctta ccatggtcca cgtgaccgat cgtgcccacg
       61 ttaacgtggg gcttggtacg ttcaaactta cccttggcca tctttggctc ctgaacaagc
      121 tcccagctcc cgggaaggaa ctggaattat ttaaagagaa ggaaaaaaat tagtggtgcc
      181 catgatgcgg atcgaacgca tgacctctcc cttaccaagg gagtgctcta ccactgagcc
      241 acatgggcac gcttcatgga gcgggtgaag ggaatcgcag gaggagttgc tccatctgga
      301 tctagtgtcc ttccgcgctg catttgagaa attcgctttg cgaaagtttc gctggtggag
      361 agggatggat tcgaaccatc gtaggcgtaa gccaacagat ttacagtctg ccccctttag
      421 ccactcgggc acctctccgt aacgaggatt tgaattatgc agacgtcttt tcgaactgtc
      481 aatagcactt cttcaaaaag gcttaaattc cgtattacta cttaaaaaca atccccttcc
      541 cctaagatct catgcgtata cactagacaa acaaccgcat tcacaaccgc ctgcagggcc
      601 ccgcgtcaat gccttcgctc gactcgatca tcaccttcag cacgcttgcc gcgctcgtac
      661 agttcggcat catcatcagc gtcatcctgc gcgtcatgct cacgcgccac ccgcccggat
      721 cagcgtttgc gtggattctc atcaccacac ttctccccta cttcggcttc atcctctacc
      781 ttctcttcgg cgaacgtccg atcggccaac tccgagccac gcgttttcgc aagcatctcg
      841 cgcaccttaa ggagctctcg cgccagaagc tcactcctac ggggccgctg ccgcgccacc
      901 tcgccagaca ccgcacgctc atacatctgg cgacccgcat ggggaacctt cccgtaacgt
      961 cgggcaccga aatcaggctt ctctcctcct cagacgacac gttcgccgcc atcatcagcg
     1021 acattgaagc cgcctgcagc tcaatcgaca tggagttcta catctggcat ccgggcggcg
     1081 aggccgacaa ggtggccgcc tcgctcctca aggcccatga gcgcggcgtg cgcgtgcgca
     1141 ttctcgtcga cgacttcggc tcgcgcgcct tcctcaagag cgacacctgc agggcgttcc
     1201 gcaatgcagg gatcgaagtc gccaaggcca tgcccatgcg gctcctgcac ttcttcggcc
     1261 ttcagcgcgt cgacctgcgc ctccaccgca agaccatcat catcgacaac gagattggct
     1321 acacgggcag cctgaacatg atcgacccgc acacctacga cgccgcgcgc accgtcggcc
     1381 cctgggtcga cgccatggtc cgcctcaccg gccccgccgt gcagagcctg cgcgccacct
     1441 tcctgatcga ctgggccatc cagcccgacg gggacccctc agacctcgag cgggtccact
     1501 ccgtccctcc gatcccacag ctcggcacgg ccacgatcgt cacggtgccc tcaggtccct
     1561 acggtcaaaa ggacccgaac ctttacctgc tgctcgacac catcaacgcc gcacgcttca
     1621 acatcaccat cacgacgccc tacttcgttc ccaacgaagg ccttgccacg gcccttctga
     1681 gcgccgcata cagaggcgtg aagatcagac tgatcgtacc cgccaacgcc gacagcaagg
     1741 ccgtcacctg ggccgcccga cgctacttcg acgacatgct ctccgccggc gtcgaagtgc
     1801 tcctctacaa cggaggactg ctgcacacca agtccatctc cgtcgacgac gaatatgccg
     1861 tcttcggcac cgtcaacttc gacaaccgca gcattcacct caacttcgag atgatgctgc
     1921 tcatcttcga cgccggcttc attcacgacc taaaaaagct gcacgactcc tatgcggagc
     1981 agtgcagccg tctcgacccc gaaacctggt accggcgccc cgtgtccgag cgcctcaagg
     2041 aaggggcctg ctatctgatt tctcctctgc tttaaggaga gttactttcg ctcctcgata
     2101 tcctcgacgt cgttcatctt ccacttcttg ttgtccgacg tcttgatgct gagcaccgtc
     2161 gtctccgtgc gcgtctcacg cacgtcttca cgagcgccct gctcggaagc ctgacgactt
     2221 gcggcggcac ggctcatcgc ctcgtaccag tcggtggtgc cgcccgcctg cctggccttc
     2281 caccatgccc atccccaggc gacaagcccc atcaccatga cggcggcgat gaccgcaagc
     2341 accacgggca gcatcaggaa gccgagaccg acaagcacgg cgaacgaaac caacccgatc
     2401 aggatgcgaa caaaggggtt tcctgacgtc tgaaaaacat agagcggcat aagcgtcttt
     2461 ctccgatagg cctccggcgt cttcaagttc tgtggcacat tgccggagct tcacattcaa
     2521 gtataagagg cggccccgtt ccgcggcggc ccctcatcta tttccttcta gatggggacg
     2581 aatacacccc gggacaagcg ttcgggttgt aaacattcgt accctcagaa gaatgaacgc
     2641 aactatgagg ccgtttccgc cccacgaagc gtcatgtttt tgtaagatag cagaccaccc
     2701 tataattgat cgatcagcag gcatcccctc actcaaaaaa acggatgctt atccgtgcag
     2761 cttgccggtc aagcaatgct cagttttcat tcccagaaca gcttccaagc agttgtcctg
     2821 agccaccaaa gaagatttat ggacgcagat aacaaaaaca ctcttccgga cgttcagtcc
     2881 gcgcgcgacg gccgcaacat cgccatcgac cgcgtcggcg ttcgcggcat caccgttccc
     2941 attacggtgg agaccaagga agacaccctt gccaccgtcg ccaccgtcga aatgaccgtg
     3001 tcgcttcctg cagacaaaaa gggcacgcac atgtcccgct ttgtcgcgct catggaagag
     3061 cattccgaac cgctcaacgt ggacgtcgtc cgctctctca tggccgacat gctcaagcgt
     3121 cttgacgccg ttgacggcac gatcgagctt cgcttcccgt tcttcatccg caagtgcgcc
     3181 cccgtcagca agctcaccag cctcatgaac tacgaggccg cctggattgc cgacagccgc
     3241 gacggcgtca tcaccgtccg tcaggaaact gccactcccg tgacgagcct ctgcccctgc
     3301 tccaaggaaa tctcccgcta cggcgctcac aaccagcgct cccgcctgac ggcctccgtc
     3361 gagctttccg ctccgatgag cctcgaagag cagatcgcct atagcgagga ggccgcctcc
     3421 tgccagctct gggcccgcct gaagcgctcc gacgaaaagt acgtcacgga atacgcctac
     3481 gatcatccga agttcgtcga agacatcgtt cgcgacatgg ccgccgctct caacaaggaa
     3541 gagcgcgtcg tcgcctaccg cgtcgaggct gaaaacttcg aatccatcca caatcactcc
     3601 gcctacgcct acatcacgca cgacaagcgc tgatgcccgc caggcactga tacgttcaca
     3661 gcgtcggcct gttgtctgaa cggacggcag gccgttacgt tttcccgatc cctttcagat
     3721 gtgtcctcac tcccgaaaag gtacactgtg acgatcagga aaacgaaccc atcactcaca
     3781 cgacactcat catgctcgct cagcaaaaag aggctctgac ctccctcatt tccgccgcgc
     3841 tcgaaacgct cggcgtcacc ggcgtcaccg tcattctcga acgcccgaag atgcccgaac
     3901 acggcgacgt cgcctgtacg gtcgccatgc agctcgctcg cgccctcaag aagaacccgc
     3961 gcgagatcgc cacgcagatc gtcgaggccc tcaaggccaa ccccgaaagc gaagctctca
     4021 tcaagtcggt cgaaatcgcc ggccccggct tcatcaacct ccgcattcag gacaacgccc
     4081 gccaggaaat catccgtcag gtcctcaagg ccggcacgtc cttcggctgc ggcgacgccc
     4141 acaagaacca gtccgttctt cttgagttcg tgtccgccaa cccgaccggc ccgcttcatc
     4201 tcggccacgc ccgtcagggc gccctcggag acgtgcttgc caatcttctc gccactcagg
     4261 gctggaaagt cacccgcgag ttctactaca acgacgccgg cgttcagatc ggcaatctcg
     4321 ccgaatccat ccgcctgcgc gcccgcgagc tcaagggtga agccgtcgaa cttcctgaaa
     4381 acgcctatca cggcgaatac gtgatcgcca tcgcccgcga ctaccttgac tgcaagcccg
     4441 tgcatgcgca tgacggcacg gtcatcgaat ccaagggcga cctttccgac accgactgca
     4501 tccgtcgcta cggcgtcgcc tatctgcgca acgagcagga cgacgacctt gatgcgctcg
     4561 gcgtacactt cgacaacttc tacctcgagt cctcgctcta cgcctccggc cgcgtgcagg
     4621 ccgccgtgca gtccatgatc gactccggcc gcaccgaaga acgcgacggc gccctctggc
     4681 tcaagaccac cgaatgcgaa gacctcggca ttcacgacga caagaaccgc gtgatgcgca
     4741 aggtcgacgg cacctttacc tactttgtgc ccgacgtcgc ctaccacctt gccaagttcg
     4801 agcgcggctt caccaaggcc gtcaacattc agggctccga ccatcacggc acgattgccc
     4861 gcgtccgctg cggccttcag tgctgcgcgc tcgcccgtca gatgagcatc ccgaaggaat
     4921 tccccgaata catccttcac aagatgctct ccgtcgtgaa ggacggcgaa cccgtcaaga
     4981 tgagcaagcg ctccggcaac tacgtcacgc tgcgcgacct cgtcgacatg gccggccagg
     5041 acgccgcccg cttcttcctc gttgcccgca aggccgatgc cgaattcgtc ttcgacatca
     5101 acctcgccca gcagaagagc gacgaaaacc ccgtctacta cctgcagtac gcccacgccc
     5161 gcatctgctc cgtgtttgcg aacgccaagg aaaagggcta cgaagttccg tccgccgacg
     5221 aactcatcaa ggccgatctc tctagcctcg tgagcgagca ggccatggac ctgatggcca
     5281 agatcgccga atatccgtcg cttctcacgc tggccgccaa ggaccatgcg ccgcacatgc
     5341 tctgctacta cctgaaggag ctcgccgcgg ccttccacac gttctacaac gccgaacgcg
     5401 tcgtgatcga gaacgatgtc gagcgcaacg cccgcctcgc tctccttgcc gccacccgcc
     5461 aggtgctcgg caacggtctc gcgcttctcg gcatcagcgc gcctgaatac atggcccgcc
     5521 gcgaagatgc ttaatcaaga ggactatcca cagtggcccg taccaatctt gcttccggct
     5581 ttgccggctt tctgatcggc gtcgtcaccg gctgcgcaat ctgcgcagcc gcggcggtgt
     5641 tcatcaccaa cagcccgatc cccttcgtcg agaaggttca gaaggtgacc gctgacgtcg
     5701 accccgccca gaagctcgcg ggcggcatcg atccgaaccg cgccctcaat gagcagcagg
     5761 aaaagagcgc cgaacaggac gattcgtccg gcgtcaagac cgtgaccgtc gctccgccgg
     5821 tccagtccca gcctcagtct cagacgcccg ccgccggagc tggcggcagc tactggctgc
     5881 aggccggtgc tttcaaggaa gagaagagcg cccatgacct tacgggctcc ctcgccctca
     5941 ccgcaggcat cagcaatgtg caggtggtga aggcgggcag cgtctggcgc gtccgcatcg
     6001 gccccttcga caacaagctg gaggcgtctg aagtccagag ccaactcgac aatcagggca
     6061 ttcagaccac ggtggtccgc tgagtcctct agagtccaag caaaaagagc agtcctcatg
     6121 ggctgctctt ttttttgttt catgcggatc cccgtcggct cagctttccg ccgccacgat
     6181 gtcgagcacg gcgtcgccgt agttgacgag cttcttctcg ccaacacctg agatgcgtct
     6241 cagggcctca ggggacgtcg ggcgctcgcg cactatctcc agcagggtcg catccttgaa
     6301 gatgacgtag ggaggcttgc cgaggctctt cgcctcatca ctcctccact ggcgcagcgc
     6361 ttcgtagagg cttctgtcac gc
//
LOCUS       DBMDTI010000105         6369 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3110_length_6369_cov_2.688098, whole genome shotgun sequence.
ACCESSION   DBMDTI010000105 DBMDTI010000000
VERSION     DBMDTI010000105.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3110_length_6369_cov_2.688098"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            976..1764
                     /gene="lpxA"
                     /locus_tag="ACZPB6_06145"
     CDS             976..1764
                     /gene="lpxA"
                     /locus_tag="ACZPB6_06145"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR1184081.1"
                     /translation="MAQIHPSSIVHPDAVLAADVVVGPFCLVGPKVRIGAGTHLRSHV
                     CIEGRTTIGSGNVIYAGASIGCDPQDKKYAGEDTVLVVGDNNVIRENCTFSIGTIQDE
                     GVTTVGSDNLFMANVHVAHDCRVGSHTIIANNVALAGHVRVDDWAIVGGQTGVHQFVR
                     IGEHAMVGGASAVLRDVPPYVICSDNPCAPHGLNTVGLRRFGYSDTQVRALHQAYRLM
                     YREGLIVKEALVKIEALKADFPDAVEQLTRFIEFIGSSPRGVIR"
     gene            1823..2998
                     /gene="lpxB"
                     /locus_tag="ACZPB6_06150"
     CDS             1823..2998
                     /gene="lpxB"
                     /locus_tag="ACZPB6_06150"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR1184082.1"
                     /translation="MGIQSSIPTLERNGAVWLAGEASGDFIASLVIPEVAARMGGAPQ
                     YGVGGDKMRAAGFRAWYDSRELSVRGYVEVLTHLPRLLSFRKDLITRFGDSMPQVFVG
                     VDAPDFNLAVEEKLRRRGVPTVHFVSPSIWAWRPERIQTIRRAVDHMLLVFPFEQEIY
                     RKAGVPATFVGHPLAGIIPMTPDTAGARRSFGIGDDGLPVITVMPGSRVDELKGCAPV
                     FFNAVERLLARNGGAHVLIPAVDEQARTRILQVASMYPRLAQAMQIVTGESHRTIEAA
                     DAVLVASGTAALECALYKKPMVVGYKMPALTGMIMQKKALIHCVSLPNILLGENVVPE
                     FLQFFCEPDPISYALEDALQNEARRRMLEERFSAMHESLIADTPALAADVIMSVARS"
     gene            3102..3779
                     /gene="rnhB"
                     /locus_tag="ACZPB6_06155"
     CDS             3102..3779
                     /gene="rnhB"
                     /locus_tag="ACZPB6_06155"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR1184083.1"
                     /translation="MAKSAASKTQPTTDLPSLFGSEDPLFSSTLVAGVDEAGRGPLAG
                     PVCAAAVILDPDRPVEGLNDSKKLSAARREALAPLIRERALAWCVAWADVHEIDTLNI
                     LQATMLAMKRAVEGLGVRPELILVDGNRTPQGLPAPASAVVKGDARVAAISAASILAK
                     TARDARMAELDKIYPGYGFAAHAGYGTARHVEAIRTLGITPEHRRSFEPVKSMIAGKA
                     AKENEND"
     gene            3772..4587
                     /locus_tag="ACZPB6_06160"
     CDS             3772..4587
                     /locus_tag="ACZPB6_06160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1184084.1"
                     /translation="MIEKFIESEANAKFKRWKKLARDARAVRKERATLMEGIHLMQVA
                     LESGCRVNAVLIDADRASREARELALELCRKSGADLFALSPALYDQLSPVEHGVGVMC
                     EAAVPPAPADDAWKSADALYLDGVQDAGNAGTLIRTAVAAGVRVVAASPVAATLWSPK
                     VMRAGMGAHFGAVFIENITPEAFRRAYRGRIYAADARGGEDLFLAADDVAEGPVCWLM
                     GAEGPGVSDAGLEAADRRFYIPIEAACESLNVGAAAAVCLFDTRRRRFARRGA"
     gene            4951..6111
                     /locus_tag="ACZPB6_06165"
     CDS             4951..6111
                     /locus_tag="ACZPB6_06165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1184085.1"
                     /translation="MLNFAYHNPVRLVFGRGAENEAGRLAETVARTHKCLVVYGGGSV
                     VRSGLLASVRKSLEAAGMTVIEKGGVHPNPRLCFVRETVDWMRDQGVGLVVAVGGGSV
                     IDTAKCIALGLEYEGDCWDFFDGHAVPEKALPVLAVLTIPAAGSEQSVRCVISHHGTK
                     AGIGAECLRPAAAIVNPECFMTLPRHQVAAGVVDMISHIMERYFTRTTATAYVDAQSE
                     AAMRTAIEFGPKVWADARDYDSWCQIGMVGSFAHNGYFGLGREEDWACHAIEHEISGW
                     NEAITHGMGLSVVIPAWMRYVSERTPARLAQFARNVFGIVEADDAEAAKKGIEALVAF
                     YKSLGMPTRMGELGADECPVESLARHCCRRGPVGRLLPLEASDVEAILKNAF"
BASE COUNT         1044 a         2193 c         1994 g         1138 t
ORIGIN      
        1 tacttttttt tcttggaggg agccgaccag actttttctt aaaagtcaag gccgcgttcc
       61 acggacttcc gagatgaacc tctttttttg cttcttcgga gcctcgggtt tgcctgcttc
      121 gtcgggctgc ggcggaatcc cgccaaaatg cgtcagaatg caagtgcgtt ccacgaggcc
      181 gtgcagcgca gtcgggcgat atatccctct gcacgaggcg tcttgtgtgc ggagggcagg
      241 gaggcggagc ggcgcggcag gaagctctgt tacagaaggc ccaatgacga aacagcaccg
      301 ggaggccctt caggtacagt caatgcatca ggaaggcgaa gctgccctcc ggagacttca
      361 agaacatgat ttcggcctgc aggaaaacga ctgcaggcct tgtttttgac ctcggcgggg
      421 cgcttgcaac gagatgcaag aatcctgcgg ggcgttcttc gctcaaagtc atgcggggcg
      481 cctcctgcag aggcgtttca agggtttgtt ccgagatgct cgaagtgttt cgttccgacc
      541 tcttgttaca tctcccggga agttcgaaac ggatggcggg cgggattcgg ctcattgaag
      601 gcatggagaa cgccgcagca atgcatgagt cctccgctcc ttttggttgg aaaacttcag
      661 ctcgcagtcc caaggaccgc tggcctcttt ttttgcgttc gccctgcacg cgtgttcctg
      721 aggaggaacg ccctgaaacg caagcggcgt atggcatttg cggcgggagg ctcctcgccg
      781 gggcgcgatc cgtcttctcg gcgcacgggc agtctaatct ataatattgg gattgctcgg
      841 cttccgcccc tgcgggagcc tgcgcgcacg ggcgcccgtg ctccgtttca cgagaacgtc
      901 agatgcacgg cgggcggcgg gatgccgcga aatttccgaa gaaggcgcgc gaacaaggtc
      961 aaaaaaggaa ttgtcatggc tcaaattcat ccgtcctcca tcgttcatcc cgatgccgtg
     1021 ctcgccgccg acgtcgtcgt cggccccttc tgcctcgtcg ggccgaaggt tcggatcggt
     1081 gcgggcactc acctgcgcag ccacgtctgc atcgagggcc gcacgacgat cggcagcggc
     1141 aacgtgatct acgcgggcgc ttcgatcggc tgcgatccgc aggacaagaa gtatgcgggc
     1201 gaggacacgg tgctcgtcgt gggcgacaac aacgtgatcc gcgaaaactg cacgttctcg
     1261 atcggcacga ttcaggacga gggcgtcacg acggtcggca gcgacaacct cttcatggcc
     1321 aacgtccacg tggcacacga ctgccgcgtc ggcagccaca cgatcatcgc caacaacgtc
     1381 gcgctcgcgg gccacgtccg cgtcgacgac tgggcaatcg tgggcggcca gacgggcgtg
     1441 catcagttcg tgcgcatcgg cgagcacgcc atggtgggcg gcgcctccgc cgttctgcgc
     1501 gacgttccgc cctacgtgat ctgctccgac aacccctgtg cgcctcacgg cctcaacacc
     1561 gtgggcctgc gccgcttcgg ctactccgac acgcaggtgc gcgcgctgca ccaggcctac
     1621 cgcctgatgt accgcgaggg cctcatcgtc aaggaggcgc tcgtgaagat cgaggccctc
     1681 aaggccgact tcccggacgc cgtcgagcag ctcacccgct tcatcgaatt catcggcagc
     1741 agcccgcgcg gcgtgatccg ctgatccgcc cggcgcattc attctcacca ctacgaaccg
     1801 taccatcaac gaggggaaac tcatgggcat tcagtcttcc attccgacgc ttgaacgcaa
     1861 cggcgccgtc tggcttgccg gcgaggcgag cggcgacttc atcgccagcc ttgtgattcc
     1921 tgaggtggcc gcacgcatgg gcggtgctcc gcagtacggc gtgggcggcg acaagatgcg
     1981 cgccgcgggc tttcgcgcct ggtacgactc gcgcgagctt tccgtgcgcg gctatgtcga
     2041 agtgctcacg cacctgccgc gcctgctgag cttccgcaag gacctcatca cccgcttcgg
     2101 cgactcgatg ccgcaggtgt ttgtgggcgt cgacgctccc gacttcaacc tcgccgtcga
     2161 ggagaagttg cgccgccgcg gcgttccgac cgttcacttc gtgagcccct cgatctgggc
     2221 ctggcgtccc gagcgcatcc agacgatccg ccgcgccgtc gaccacatgc tgctcgtctt
     2281 cccgttcgag caggagatct accgcaaggc cggcgtgccc gccaccttcg tcggccatcc
     2341 gctcgcgggc atcatcccga tgacgcccga cacggcgggc gcccgcagga gcttcggcat
     2401 tggtgacgac ggcctgcccg tcatcaccgt gatgcccggc tcgcgcgtcg acgagctcaa
     2461 gggctgtgct ccggtcttct tcaacgccgt cgagcgtctt ctcgcccgca acggcggcgc
     2521 ccacgtcctg attcccgccg tcgacgagca ggcccgtacc cgcatcctgc aggtggcgag
     2581 catgtacccg cgccttgccc aggcgatgca gatcgtcacg ggtgaaagcc atcgcacgat
     2641 cgaggccgcc gacgcggtgc tcgtcgcctc cggcacggcc gcgctcgagt gcgcgctcta
     2701 caagaagccg atggtcgtgg gctacaagat gcccgccctc acgggcatga tcatgcagaa
     2761 gaaggcgctc atccactgcg tttccctgcc gaacatcctg ctcggcgaaa acgtcgtgcc
     2821 cgagttcctg cagttcttct gcgagcccga tccgatctcc tacgcgcttg aggacgcgct
     2881 tcagaacgaa gcccgccgca ggatgctcga ggaacgcttc tcggccatgc acgagagcct
     2941 catcgctgac acgcccgctc tcgccgccga cgtcatcatg agcgtcgcaa gatcctgagg
     3001 ggctcgagag ctctgagaga gggcaagccc gcaggggcgc ttcgccggcc ggttccgcgg
     3061 agcgcctttt ctttttccca ttcattcacc aggacctgac cgtggccaaa tccgccgctt
     3121 ccaaaacgca acccaccacc gacctgccga gcctcttcgg ttcggaggat ccgctctttt
     3181 catcgacgct cgtcgcaggg gtcgacgagg cgggccgcgg cccgctcgcg ggccccgtct
     3241 gcgcggcggc cgtgattctt gatcccgacc gtcccgtcga gggcctcaac gactcgaaga
     3301 agctctcggc cgcgaggcgc gaggcgctcg cgccgctcat tcgcgagcgc gcgctcgcct
     3361 ggtgcgtcgc ctgggccgac gtccacgaga tcgacacgct gaacatcctt caggcgacga
     3421 tgctcgccat gaagcgcgcc gttgaggggc tcggcgtcag gcccgagctc atcctcgtcg
     3481 acggcaaccg cacgccccag gggctgcccg ctcccgcctc cgccgtcgtg aagggcgacg
     3541 ccagggtcgc cgccatctcg gccgcctcga tcctcgcgaa gaccgcacga gacgcccgca
     3601 tggccgaact cgacaagatc tatccgggct acggcttcgc cgctcatgcg ggctacggca
     3661 ccgcaaggca cgtcgaggcg atccgcacgc tcggcatcac gcccgagcac cgccgctctt
     3721 tcgagcccgt caagtccatg atcgccggca aggcagccaa ggagaacgag aatgattgaa
     3781 aagttcattg aaagcgaggc caacgcgaag ttcaagcgtt ggaaaaagct cgcgcgcgac
     3841 gcgcgtgccg tgagaaagga gagggccacc ctgatggagg gcatccatct gatgcaggtg
     3901 gcgctcgaat ccggctgccg cgtcaacgcc gttctgatcg atgcggaccg cgcctcgcgc
     3961 gaggcccgcg aactcgccct tgagctctgc aggaagagcg gcgcagacct ctttgcgctc
     4021 tcgcccgccc tctacgacca gctttcgccc gtcgagcacg gcgtcggcgt catgtgcgag
     4081 gcggccgttc cgcccgcgcc cgccgacgac gcctggaagt ccgccgacgc gctctatctc
     4141 gacggtgtgc aggacgcggg caacgcgggc acgctgatcc gcactgcggt cgccgcaggc
     4201 gttcgcgtcg tggcggcaag tcccgtcgcc gcaaccctct ggtccccgaa ggtgatgcgc
     4261 gccggcatgg gcgcgcactt cggcgccgtc ttcatcgaga acatcacgcc cgaggccttc
     4321 cgcagggcct atcgcggccg catctacgcg gccgacgccc gcggcggcga ggatctcttc
     4381 ctcgcggcgg acgacgttgc cgagggcccc gtttgctggc tgatgggcgc cgagggacca
     4441 ggcgtctccg atgcgggcct tgaggctgcc gaccgtcgct tctacattcc gatcgaggcc
     4501 gcgtgcgaaa gcctcaacgt gggcgccgcg gccgccgtct gcctcttcga cacccgccgc
     4561 aggcgctttg cgcgcagggg cgcctgacgc tcgcgtgatg cggtgaaggg tgggggaccg
     4621 ggtgctcctt cgccctcgca gggtgtcgtg cgaacgcgtc ttccgacgcc tgcacgcctc
     4681 ccgcacgggg cttttcgggc ggctggatct gcggtcgtga tgccttttcc gcgaagaaag
     4741 cgggtgttgc atataggaga aaatccctaa agaaaaaccc gaatttttca tgcagggcgg
     4801 ccgtcaaacg gataaactgc gcacctgacg tcgccgggag tgccaggagc gcagaccccg
     4861 gcgtttattt ttttcggaaa aaaatacaag gccgccggcc gccgtccctc ccggcatgaa
     4921 gaggagggga cggaggcccg cctcacaagc atgctgaact ttgcctacca caatcctgtc
     4981 cgcctcgtct tcggacgcgg cgccgaaaat gaagccggtc gtctggctga aaccgttgcc
     5041 cgcacccaca agtgccttgt ggtctacggc ggcggctccg tcgtccgctc cggccttctc
     5101 gcctcggtga ggaagagcct cgaggccgca ggcatgacgg tgattgaaaa gggcggcgtc
     5161 catcccaacc cccgcctgtg cttcgtgcgc gagacggtcg actggatgcg cgatcagggc
     5221 gtgggtcttg tcgtcgccgt gggcggcggc tccgtgatcg acaccgccaa gtgcattgcc
     5281 cttggcctcg agtacgaggg cgactgctgg gacttcttcg acggccacgc cgttcccgaa
     5341 aaggccctgc ccgtgctcgc cgtgctgacg atccccgccg cgggttccga gcagtccgtg
     5401 cggtgcgtga tctcgcacca cggcacgaag gccggtatcg gcgccgaatg cctgcgcccc
     5461 gccgcggcca tcgtcaatcc cgaatgcttc atgacgctgc cgcgccatca ggtcgccgcc
     5521 ggcgtcgtcg acatgatctc ccacatcatg gagcgctatt tcacgcgcac gaccgcgacg
     5581 gcctacgtcg acgcgcagtc cgaggctgcc atgcgcaccg cgatcgaatt cggcccgaag
     5641 gtctgggcgg atgcccgcga ctacgattcc tggtgccaga tcggcatggt cggcagcttc
     5701 gcgcacaacg gctacttcgg tctgggccgc gaggaggact gggcctgtca cgccatcgag
     5761 catgaaatct ccggctggaa cgaggccatc acccacggca tgggcctctc cgtcgtgatc
     5821 cccgcctgga tgcgctacgt ctctgaacgc acgcccgcgc gcttggcgca gttcgcccgc
     5881 aacgtcttcg gcatcgtcga ggccgacgac gccgaggctg cgaagaaggg tatcgaggcg
     5941 ctcgtcgcct tctacaagtc cctcggcatg ccgacccgca tgggcgagct cggcgccgac
     6001 gaatgccccg tcgagtcgct cgcgcgccac tgctgccgcc gcggccccgt gggccgcctc
     6061 ctgccgcttg aggcgtccga cgtcgaggcg atcctgaaga acgccttctg aagctgatgc
     6121 ttcgaacctc ccgaggttca ggctgaatcc ggggcaacgg gccgcgcact ttcctcacgt
     6181 gaaggcgcgg cctttttcac atccgtcttc gccgccgccc gcgcacgccg tcggacgccc
     6241 tttcccacat cttgtgccgg cgcacctccc cgagttcggc tgcctcctcc ttcacccgct
     6301 tgtaaaaccc tctcccccat tgctttcgac cgttgttcgg ggcgtcattt ctctggagtg
     6361 aaatctgtg
//
LOCUS       DBMDTI010000106         6341 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3123_length_6341_cov_2.033814, whole genome shotgun sequence.
ACCESSION   DBMDTI010000106 DBMDTI010000000
VERSION     DBMDTI010000106.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3123_length_6341_cov_2.033814"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="ACZPB6_06170"
     CDS             <1..725
                     /locus_tag="ACZPB6_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR1184086.1"
                     /translation="SGLPGMAPALIVYNKGESDNTIAFATRAKDGFFLQGLYTNGSND
                     SETEYGWSDNNHVAQVAFGWTGRQLRIGSLLNWEMPEHTDATQARKHDNFGFTILGSW
                     DFGGPAVSAILYKGKNDWRIGGAPDLGAILLKGSGVNNLAQSTEGLDVTTGFVSFGYP
                     YGFHYFSAGLGVVNAEWNGVSAGMKHTKGTAAIGSLMYYYNLSKRTQLYAGASYANGH
                     DLLDGVDRFNQVFASGGLTYRF"
     gene            996..1646
                     /locus_tag="ACZPB6_06175"
     CDS             996..1646
                     /locus_tag="ACZPB6_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR1184087.1"
                     /translation="MFKKTLLAVGLSLFAAASMAATEGTDYVKLEKPMVNAEGKLTKI
                     FSYDCPFCFKYDVGVDPKVLPRIEKETGLAFNPIHLETKGKYGRIGSEFLAMCLLRDK
                     AAGVSVESKDSLFKKAKDAVYVAYHKKGERWTSGEAAFLKTMTDATGISADEFAKAKN
                     DPAVKAMCDEWKLSYDVAKIQGIPAYVVNGKYLIMTKSIRSLDGMVELVKELKDMK"
     gene            1849..2517
                     /gene="dsbI"
                     /locus_tag="ACZPB6_06180"
     CDS             1849..2517
                     /gene="dsbI"
                     /locus_tag="ACZPB6_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HIR1184088.1"
                     /translation="MIKFMKGLGSDPVRTLADWQDSRWPWGIIAFAMVAMVLLAHYVF
                     QDWMHMLPCEQCVYIRYGNLVMALGCVIAMIDPKAVWAKLVGFVITIYGLVYTIICSV
                     KLIGIHDAVHSDDPEAMFGMQGCSTEPHFPFGLPLEKWAPDWFQPTGDCGYDAPVVPD
                     GVELGALQRWFIELYQSYDGWYLIPQWKFMDMASCCLLACIVAAAFIAAMLFGWGARL
                     TKKA"
     gene            complement(2617..3582)
                     /locus_tag="ACZPB6_06185"
     CDS             complement(2617..3582)
                     /locus_tag="ACZPB6_06185"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1184089.1"
                     /translation="MAGKETLPDNGVLLTLASLSRTRSLTLTGSELGTSTATVSRTLA
                     AARAFFGDELFVRSGPRMVPTARMRALQPAVDAVLRQLEALSAPAVFDPAGIERTFRI
                     AAADNGILAFVFPALKAVLEQAPGLRLEIIGINGDIFDDLRNGRLDLAVFPFDPIPPE
                     CRSVVLADEPDVLIVRSGHPLVRISRERGALTLEDLRAYPKVRISNVTHNFSAAVVRF
                     HASLMAHEREGARITMPYFLTAPWIVLETDATAKILEKNARMLARYLPIEILPLPPGL
                     AGRYERRVIWHENASGDPALMWLIAMLRSASAQEACAGGARRPEG"
     gene            3860..5653
                     /locus_tag="ACZPB6_06190"
     CDS             3860..5653
                     /locus_tag="ACZPB6_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HIR1184090.1"
                     /translation="MKLIRLTAAAALAALASSAVFAESMPPAPTPKGTHLGNVIVNPY
                     GFSPLTAVINRNTKHPTDIRVTVKGKPEGGVDITYDVGRSTLLSNDGIPVFGLYPDYR
                     NTVVVSYKLDGRAITDTYSIVTPALTMPYMDNRNITALPQPKVTKLDTAFRDRLYLVN
                     SGTRPPKGSEVNWIMPKTPQMSKSAANPSGGSLAFETIPLNYVVDTQGEFRWWLEPQA
                     IYDGINYDIADRGYAMGFFPTKSGTYTFVQGQRWIEMNLAGRIVAKHPLPPGYIDLSH
                     ASWEMPNGNILLRAAKYRYHRPDGQIVQSVRDFIIEVDRSGNVIDEWDLNTILDPTRD
                     ALIKNLDLGAVCMNVDLDAQGKAVGAEPLAPFGDAAGVGTGRNWAHVNSVAYDESDDS
                     IIISARHQGVMKIGRDKEVKWILAPSEGWKNGLEKKLLKPVDKDGRPLKCSSTGDCEG
                     GFDFTYTQHAAWPRASKNGTIVVFDNGQVRHYEQPALPEMNYSRVVEYKIDPKTMTVK
                     QTWAYGKDRGYESFAPITSNAAYQADKDTMFGFYGSVGLFDQTKGTIGRLQEVDYKTG
                     DVKVEIDVYNNKASAPHYQGHIIDLKQAFAK"
     gene            5821..>6341
                     /locus_tag="ACZPB6_06195"
     CDS             5821..>6341
                     /locus_tag="ACZPB6_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HIR1184091.1"
                     /translation="MHYPIEVAGLKRDLPLFPISDELQIAAFIMLGDQELTVACASAL
                     LEKAPEYDYMLTAEAKGIPLIHEMARQTGAARYFVARKKMKLYMGEALEVDVQSITTA
                     GMQKLFLTEEDAALMKGRRILIVDDVISTGKSLEALEKLVEKAGGIVVGRAAVLAEGE
                     AAERTDITFLEPLP"
BASE COUNT         1216 a         2216 c         1817 g         1092 t
ORIGIN      
        1 gctcgggcct tccaggcatg gcacccgcgc tcatcgtcta caacaagggc gagtccgaca
       61 acacgatcgc cttcgcaacc cgcgccaagg acggcttctt cctccagggc ctctacacca
      121 acggctcgaa cgacagcgaa accgaatacg gctggtccga caacaaccac gtcgctcagg
      181 tcgccttcgg ctggacgggc cggcagcttc gcatcggctc gctcctcaac tgggagatgc
      241 ccgagcacac cgacgcgacg caggcccgca agcacgacaa cttcggcttc acgatcctcg
      301 gcagctggga cttcggcggt ccggccgtct ccgccattct ctacaagggc aagaacgact
      361 ggcgcatcgg cggcgcaccc gacctaggcg ccatcctcct caaggggagc ggcgtgaaca
      421 acctcgcgca gagcaccgaa ggcctcgacg tcacgacggg cttcgtgagc ttcggctatc
      481 cctacgggtt ccattacttc agcgccggcc tcggcgtcgt caacgccgaa tggaacggcg
      541 tctctgccgg catgaagcac acgaagggca cggccgccat cggcagcctc atgtattact
      601 acaacctctc gaagcgcacc cagctctacg cgggcgcctc ctacgcgaac ggtcacgacc
      661 tccttgacgg cgtcgaccgc ttcaaccaag tcttcgcttc gggcggcctc acgtaccgct
      721 tctgacattc ccgttctctt tgagacattc ggaccgccgc cggccgcttc gtgccgacgg
      781 cggtcctttc gcttcatgcg gtctgagggc ggaagaggcg gggccttttg tattttctgc
      841 aacaaggcct tgcacaacct gccgtgcgta cttggaaaag tcacaatagc ccgaataaaa
      901 ggggaggtct aaactcgcag tcatggttca ggcgagcccc gcacacatcc gacgttccgc
      961 cgaccacccg tcacccatct ggagaaaact cacaaatgtt caagaagact ctgcttgccg
     1021 tcggcctctc tctctttgcc gctgcttcca tggccgccac ggaaggcacc gactacgtca
     1081 agctcgaaaa gccgatggtc aacgccgaag gcaagctcac caagatcttc agctacgact
     1141 gcccgttctg cttcaagtat gacgtcggcg tcgacccgaa ggttctgccg cgcattgaaa
     1201 aggaaaccgg ccttgccttc aacccgatcc atctcgaaac caagggcaag tacggccgca
     1261 tcggcagcga attcctcgcc atgtgcctcc tcagggacaa ggccgcaggc gtctccgttg
     1321 agtccaagga ctctcttttc aagaaggcca aggacgccgt ctacgtcgcc tatcacaaga
     1381 agggcgaacg ctggacttcc ggcgaagccg ccttcctcaa gaccatgacc gacgccacgg
     1441 gcatcagcgc cgatgagttc gccaaagcca agaacgatcc ggccgtcaag gcgatgtgcg
     1501 acgaatggaa gctctcctac gacgtcgcca agatccaggg catcccggcc tacgtcgtca
     1561 acggcaagta cctgatcatg acgaagagca tccgcagcct cgacggcatg gtcgagctcg
     1621 tcaaggaact caaggacatg aagtaagccc tgccgctccg ccttcgcgga gcagggcccg
     1681 acaggttctc cctgggcggg agggacggtg gtacgtccgt ctctcccgtt tttcgtcttc
     1741 ggatttcgtc tccccgacac ccgacatttt cacacccgac agggtccgaa aagggcaagg
     1801 gactgtgccg gaatcttttt cggccatcaa caaaaggatt ttttcatcat gatcaaattc
     1861 atgaaggggc tcgggtctga tcccgtccgc acgctcgccg actggcagga ctcccgctgg
     1921 ccctggggca tcatcgcgtt tgcgatggtc gccatggttc ttctcgcgca ctatgtcttt
     1981 caggactgga tgcacatgct gccctgcgag cagtgcgtct acatccgcta cggcaacctt
     2041 gtgatggcgc tcggctgcgt catcgccatg atcgacccga aggccgtctg ggcgaagctc
     2101 gtcggcttcg tgatcacgat ctacggcctc gtctacacga tcatctgctc ggtcaagctc
     2161 atcggcattc atgacgccgt gcactccgac gatccggaag cgatgttcgg catgcagggc
     2221 tgctcgaccg agccgcactt cccgttcggc cttccgctgg aaaagtgggc tccggactgg
     2281 ttccagccca cgggcgactg cggctacgac gcgccggtgg tccccgacgg cgttgagctc
     2341 ggcgccctgc agcgctggtt catcgagctc taccagtcct acgacggctg gtacctgatc
     2401 ccgcagtgga agttcatgga catggcctcc tgctgcctcc tcgcctgcat cgtcgcggcc
     2461 gccttcatcg ccgccatgct cttcggttgg ggcgcccgcc tcacgaagaa ggcgtaatcg
     2521 aacccgaacc cgggcgtgaa acgggccgtc ggcgaaagcc ggcggcccgt ttcttcgtgc
     2581 ggcgtcttcg tgccttttcg tcgtgcggcc ttccgatcag ccttccggcc tgcgcgcgcc
     2641 gcccgcgcag gcttcttgcg ccgaggcgct tctgagcatc gcgatgagcc acatcagggc
     2701 cggatcgccc gaggcgttct cgtgccagat cacgcgccgc tcgtagcggc ccgccagccc
     2761 gggcggcagc ggcaggatct cgatcggcag gtaccgggcg agcatgcggg cgttcttctc
     2821 gaggatcttc gccgtggcgt ccgtctcgag cacgatccaa ggcgcggtga gaaagtaggg
     2881 catcgtgatg cgggcgccct cgcgctcgtg cgccatgaga cttgcgtgaa agcgcacgac
     2941 ggcggcggag aagttgtgcg tgacgttgct gatgcgcacc ttcggatagg ccctgaggtc
     3001 ctcgagcgtg agcgcgcccc gctccctgct gatcctcacg agcggatgcc cgcttcggac
     3061 gatcagcacg tcgggctcgt cggcgagcac cacgctgcgg cactcgggcg gaatcgggtc
     3121 gaacggaaag accgccagat cgagcctgcc gttgcgcagg tcgtcgaaaa tgtcgccgtt
     3181 gatcccgatg atctcaagcc tcaggcccgg tgcctgctcg agcaccgcct tgagcgccgg
     3241 aaatacgaag gcgaggatgc cgttgtccgc ggccgcgatc cggaaggtcc gctcgatgcc
     3301 cgccggatcg aacaccgcgg gcgccgagag cgcctcgagc tgccggagca ccgcgtcgac
     3361 cgcgggctgc agagcgcgca tgcgcgccgt cggcaccatc ctcgggccgc ttcgcacgaa
     3421 aagctcgtcg ccgaagaatg cgcgcgccgc ggcgagcgtg cggctcacgg tcgcggtcga
     3481 agtgccgagc tcgctcccag tgagcgtcag actgcgcgtg cgcgacaagg aggcgagcgt
     3541 cagaagcacg ccgttgtcgg gaagcgtctc ttttcctgcc aaggtcctca tcctcctgat
     3601 ctgttggtcc tccgcccgaa gggaagggcg gtcaagtctt tattttgaca catccgcaac
     3661 ggcggaaagt gcaccgttgc agtttgcgta atctcccgtt gcgtgccccg tccatccaat
     3721 gtgggtatca ggcaatgtcg cccgaaggcg tccctcgcta cactcagtgt cgaccttcgg
     3781 ccccgtctcc ggaggactct tccgcccgac gggcccgcac ttcgaccgac atcatgcttc
     3841 acaaagagga gacctgccca tgaagctcat ccgtctcacc gcagccgccg cccttgcggc
     3901 gctcgcatcc tccgccgtct tcgccgaatc gatgccgccc gcaccgacgc ccaagggcac
     3961 gcatctcggc aacgtcatcg tcaatccgta cggcttctcg ccgctcacgg ccgtcatcaa
     4021 ccgcaacacg aagcacccga ccgacatcag ggtgacggtc aaaggcaagc ccgaaggcgg
     4081 cgtcgacatc acctacgacg tcggccgcag cacgctgctc tcgaacgacg gcattccggt
     4141 cttcggcctc tatcccgact accgcaacac ggtcgtcgtg agctacaagc tcgacggtag
     4201 ggcgatcacg gacacctaca gcatcgtgac gcccgcgctc acgatgccct acatggacaa
     4261 ccgcaacatc accgcgctcc cgcagcccaa ggtcacgaag ctcgatacgg ccttccgcga
     4321 ccgtctctat ctcgtcaact ccggcacccg tccgcccaaa ggctccgaag tgaactggat
     4381 catgcccaaa acgccgcaga tgagcaagag cgccgccaat ccctcgggcg gctcgctcgc
     4441 cttcgagacg atcccgctca actacgtcgt cgacacgcag ggcgagttcc gctggtggct
     4501 cgaaccgcag gccatctacg acggcatcaa ctacgacatc gccgaccgcg gctacgccat
     4561 gggtttcttc ccgacgaagt cgggcaccta caccttcgtg cagggccagc gctggatcga
     4621 gatgaatctc gcgggccgca tcgtcgccaa gcatccgctg ccgccgggat acatcgacct
     4681 gagccacgcc tcatgggaaa tgcccaacgg caacatcctc cttcgcgccg ccaagtaccg
     4741 ctaccatcgt cccgacggcc agatcgtgca aagcgtgcgc gacttcatca tcgaggtcga
     4801 ccgctcgggc aacgtgatcg acgaatggga cctcaacacc atcctcgacc cgacgcgcga
     4861 cgcgctcatc aagaacctcg acctcggcgc cgtctgcatg aacgtcgacc ttgacgccca
     4921 gggcaaggct gtgggcgccg aaccgctcgc gcccttcggc gacgccgccg gcgtcggcac
     4981 gggccgcaac tgggcccacg tcaactccgt cgcctacgac gagtccgacg actcgatcat
     5041 catctccgcc cgccatcagg gcgtcatgaa gatcggccgc gacaaggagg tgaagtggat
     5101 cctcgcgccg tccgaaggct ggaagaacgg tcttgaaaag aagctcctca agccggtcga
     5161 caaggacggc aggcccctca agtgctcgtc gacgggcgac tgcgagggcg gcttcgactt
     5221 cacttacacg cagcacgccg catggccgcg cgcctcgaag aacggcacca tcgtcgtctt
     5281 cgacaacggc caggtccgtc actacgaaca gcccgcgctg cccgagatga actactcgcg
     5341 cgtcgtcgaa tacaagatcg accccaagac catgaccgtg aagcagacct gggcctacgg
     5401 caaggaccgc ggctacgaaa gcttcgcgcc catcacgtcc aacgccgcct atcaggccga
     5461 caaggacacg atgttcggct tctacggctc cgtgggcctc ttcgaccaga cgaagggcac
     5521 catcggccgc ctgcaggaag tggactacaa gacgggcgac gtcaaggttg aaatcgacgt
     5581 ctacaacaac aaggcgtcag ccccgcacta tcagggccac atcatcgacc tgaagcaggc
     5641 cttcgccaag taacgcgaat ccgcgtcacg tgaaggaggg gagacgccgt cggcggtgtc
     5701 tcccctttcc ttccttcgcg gtccgcaccg cacgaagtcc cgccgacttt gtacaatacc
     5761 cgtcgcacgc cggacgcttc cggcaccctt catcgcacaa tctcctagcg gagtcctcag
     5821 atgcattatc cgatcgaagt cgccggcctc aagcgcgacc tgcctctctt tcccatttcc
     5881 gacgagctgc agatcgcagc cttcatcatg ctcggcgatc aggagctcac cgttgcctgc
     5941 gcctcggccc tccttgaaaa ggcccccgag tacgactaca tgctgacggc cgaagccaag
     6001 ggcatcccgc tcattcacga aatggcccgc cagaccggcg ccgcgcgcta cttcgtcgcc
     6061 cgcaagaaaa tgaagctcta catgggcgag gcccttgagg tcgacgtcca gtcgatcacg
     6121 accgccggca tgcagaagct cttcctcacg gaagaggacg ccgccctcat gaagggcagg
     6181 cgcattctca tcgttgacga cgtcatctcc acgggcaagt cccttgaagc cctcgagaag
     6241 ctcgttgaaa aggcgggcgg catcgtcgtc ggccgcgccg cggtcctcgc cgagggcgaa
     6301 gccgccgagc gcaccgacat caccttcctt gaacccctcc c
//
LOCUS       DBMDTI010000107         6284 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3156_length_6284_cov_2.180171, whole genome shotgun sequence.
ACCESSION   DBMDTI010000107 DBMDTI010000000
VERSION     DBMDTI010000107.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3156_length_6284_cov_2.180171"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="ACZPB6_06200"
     CDS             <1..385
                     /locus_tag="ACZPB6_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HIR1184092.1"
                     /translation="PLLMMYGRVLDADYTTSMGVAVEHEAVDTLIGRVGFVLGLKCPN
                     NRGNTYVRASILHDWQGDAESTFTKGGQTKHISEELGDTWLEYGVGANFNATKNLHLY
                     ADLEATEGAKVETSYRFNLGARWSF"
     gene            830..2239
                     /locus_tag="ACZPB6_06205"
     CDS             830..2239
                     /locus_tag="ACZPB6_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184093.1"
                     /translation="MTLLEKCRKWHEAGQFPKIIEALEALGEDGRTPELASELARAYN
                     NEADPNTPEGRRMLHRAIELLEPLEEALGATYLWNFRLGYAYYYLDQEGRALSRFRRA
                     HEAKPEDEDAKEFMEDCLKRVTLPFFRVPFRERTQKAWTAFEGEEAEIRAMMDADKTH
                     ERGEEIVDRIERILRLAFEDVSFEVGFNGVKHELILTPEGNRMKLFELVYFRSHAPAS
                     VLRHWEITLGRRGTSGNELHAGGVRIGGEDVQAWLEPDDDAFKLSVFCARLADLKAKE
                     EGRAWWMLTTLTDQLLGEIPHMRWINDFELLDAPRAVPGFLLSDLPDKLRELGKDLAL
                     DAETLLENSYVGYTREPDPDPDADWRLDVIAGSTACPPLINGYMSADDETMDALHSDG
                     VTAGFIAFSLDGFTGENRTQRIFAFREALEAKLEKACGPDVVRMVGGATGVHFGYVDF
                     MAWDLQPVLFEARAFLEVS"
     gene            2250..4832
                     /locus_tag="ACZPB6_06210"
     CDS             2250..4832
                     /locus_tag="ACZPB6_06210"
                     /inference="COORDINATES: protein motif:HMM:NF051508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HIR1184094.1"
                     /translation="MPAASFHVFRREVGSVPLKSPDDRADAGEDDDDEPLDYRPETAE
                     AFHARIEKWNDDDEYTRCIRALDGVPTQYRDYRHAYALARALENYAVLGDGQYGCPRD
                     KAREALRRAIGVLESVREEGLDRAEWHMRMAYGYQYLTGEEEKAIPYARTWAQLDPED
                     EDAPCVIRECEKAVEARCKNDGKPVDRKGVFLGFVLLEKAHWDKGRLIADLKAEWDID
                     VPEEDKDDRKDNGDSLVFEAGDMIAAVSLMHGPVPGGEAEDNAENNYMWPDAVKAAQS
                     HAAHIMVTVLGKETDVRLRGQFFVKLAAACCRQPGVLGVYTSGVVFEPSFYADMAGVM
                     KEGELPLNNWIWFGLHRREGGVCGYTYGMDVFGKEELEVLDADADPLAVRKFLVSLAG
                     YVIGSDVTLNDGETIGFSQGDRHRITRSPGVALPDQPTFKVSVEQNEILLDEAAPHLE
                     SIREKCLPVDELNAFSHLAIYLRWCIEHDLMSESFCRRWPDIVEAVRTNPAEACLREW
                     LRDKLKGELHASIFNERGADFACYYYGALHEYTPHYTMDVDIHAVSRIGVERNFSDEI
                     QDEAYLFLPFDEDYYREMARIIERRYENWAGQRFDDSTLAPSETAEALMAYLDCECTY
                     FPSMADDDPIMCAFEYAGKNAAHEGFVPMLIAVDETLWECLMLNSDPAHEGDDDHAFD
                     PDAVAAYRQSQLAAPLPDGRMVLEDGMAERRAEAEDDSKDWDTEVVGRMEGGFNNHRF
                     SGYWNSETDMTHPVILARIPVRHPWEVFAHLPFGNWNDCPDTPQLMAVARYWFEKFGA
                     VPAVMTHDELDFALPKPIGRELAVQTALEQYAFCPDMDQNHESVGALADTLRQSKIWY
                     FWWD"
     gene            4864..5211
                     /locus_tag="ACZPB6_06215"
     CDS             4864..5211
                     /locus_tag="ACZPB6_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HIR1184095.1"
                     /translation="MKVRQWAWLLLAGAVAPFVFDNGPDPVSDGLYRVVDDHGRIGYA
                     DAYGVVRIAPRFAFGLPFANGVARVTLSGHPAAVPGSEGEYHRWEDGDWFCINKSGSI
                     VKCPSSSDHGSRP"
     gene            5208..5438
                     /locus_tag="ACZPB6_06220"
     CDS             5208..5438
                     /locus_tag="ACZPB6_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184096.1"
                     /translation="MKAFVEKLFVEWNDVICLLFGSLCILGAIRDWDWLCDPTGKPHA
                     PLLTRGLLRAVFLMLGTILVVCSVWLLLKRQN"
     gene            5448..>6284
                     /locus_tag="ACZPB6_06225"
     CDS             5448..>6284
                     /locus_tag="ACZPB6_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184097.1"
                     /translation="MNFNRETCMRLAAGTVLCGGAALGGLLWWQLQAFDRMTDSRLER
                     GMGLFALELERRDGGLLQRDLVLRLGFRTEDGLTESFLVMNGRFRPGLRPSVSFEPVA
                     NSDPDAAILVKADPRIRFVFSPLMSPEEAEVVWRGASDEKETIGDGRVTADIGFDRAR
                     RALTGLTVSGRLGASESRWEGGHVKSAEKTFEYVYAESPLKFAFSYSSAGDFVKGELM
                     PLGMGPLSYSLTVTPEEEAGRLRNATDFKFDIRDVNINDSELPVFDLRFDAGLYTPPN
                     VWL"
BASE COUNT         1225 a         1738 c         2005 g         1316 t
ORIGIN      
        1 ccctctttta atgatgtacg gtcgcgtgct cgatgccgac tatacgacgt ccatgggcgt
       61 tgccgtcgag catgaggccg tcgatacgct catcggtaga gtcggtttcg tgcttggtct
      121 gaagtgcccg aacaatcgcg gcaacaccta tgttcgcgct tcgatcctcc atgactggca
      181 gggtgacgcc gagtcgacgt tcacgaaggg cggtcagacg aagcacatct ccgaagagct
      241 cggtgacacc tggcttgaat acggtgtcgg cgcgaacttc aatgccacga agaacctgca
      301 tctctatgca gaccttgaag ccaccgaagg ggccaaggtc gagacgtcgt acaggttcaa
      361 tctgggcgca cgctggtcgt tctgagggtt attgaccttc ggggcgcttg atgaataaag
      421 gcaggagcag tccgagacct gattcctgcc ttgcagtcgc cgcgcgagac ggggcaaccc
      481 gtcagcgcgg cacttttgtt ttcgaagatg cttcagttga cctgatccgc ctcctcgcgt
      541 gtcttttcag cgagtccgct ttggcatctc catcacctca acgctccctt gggagaggaa
      601 atggaattgg ctttctgtaa gcgaaattcg ggttagcccc gtcttgcatg ggagaaaggt
      661 tgtgtgataa aaaaatgaat cgcattgtct ttcgtttgac accatgaaga cgaaatccgc
      721 agcgcgaagc attcggcgac cgcctttggg atgagcccgt caagtactgc tcatgtccgg
      781 agaacgttcg cctgaacgac caatttgccg cttgttgtcg ggagggagca tgacgctact
      841 cgaaaaatgc cggaaatggc acgaagccgg acagtttccc aaaatcatcg aggcgcttga
      901 agcgctcgga gaagacggac gaacgccgga acttgcaagc gagttggctc gcgcctacaa
      961 caacgaggcg gatccgaaca cgcccgaagg gcgccgcatg ctccaccggg cgattgagct
     1021 tcttgagccg ctcgaggagg cacttggtgc aacctatctg tggaacttcc gcctgggcta
     1081 tgcctactac tatctcgacc aggaagggcg cgcgctttca cggttccgac gggcgcacga
     1141 agccaaaccc gaggacgagg atgccaaaga attcatggag gactgtctca agcgcgtgac
     1201 gttgccgttc ttccgggttc ccttcaggga gcgcacgcag aaggcctgga cggccttcga
     1261 aggcgaggag gcggagatcc gcgccatgat ggatgctgac aagacgcatg agagaggcga
     1321 agagatcgtc gatcgcatcg agcgcattct ccggcttgcg tttgaggacg tttcgttcga
     1381 ggtcggcttc aatggcgtca agcatgagct cattctgacg cctgaaggca accggatgaa
     1441 gctcttcgag ctcgtctact tccgaagcca tgcgcccgca tccgtcctga ggcactggga
     1501 gatcacgctt ggtcgtcgtg ggacgtcggg caatgagctt catgccggtg gcgtcaggat
     1561 cggcggtgaa gacgtgcagg cctggcttga gcccgacgac gatgcgttca agctctccgt
     1621 cttctgcgcc aggcttgcgg atctgaaggc aaaggaggag ggacgtgcct ggtggatgct
     1681 cacgacgctc accgaccagt tgctcggcga aattccgcac atgcgctgga tcaacgactt
     1741 cgagctcctt gatgcgccca gggcggtgcc gggttttctt ctgagcgatc tgccggacaa
     1801 actgcgggag ctcggcaagg acctggcgct cgacgccgag acgctgcttg agaacagtta
     1861 tgtcggctac acgcgcgagc ctgatccgga ccccgacgcc gactggcgcc ttgacgtcat
     1921 cgccggttcc acggcctgcc cgcctctcat caatggctat atgagtgcgg acgatgagac
     1981 gatggatgca cttcattccg atggcgtgac ggcgggattc atcgcctttt cgctcgatgg
     2041 ctttacgggc gagaatcgga cgcagaggat ctttgctttc cgtgaggcgc tggaggcgaa
     2101 gctcgaaaag gcgtgcggac ccgatgtcgt caggatggtc ggcggcgcta ccggcgtgca
     2161 tttcggctat gtcgacttca tggcctggga tctgcagccc gtgttgttcg aagcccgggc
     2221 gtttcttgag gtttcttgag gcatcggacg tgccggcggc gagctttcac gtctttcgcc
     2281 gcgaagtggg atccgttccg ctcaagtctc ccgacgatcg ggcggatgcc ggcgaggatg
     2341 acgatgatga accgctcgac tatcgtcccg agacggcgga ggccttccat gcccggattg
     2401 aaaaatggaa tgacgatgac gaatatacgc gctgcattcg tgcgctcgat ggcgttccaa
     2461 cgcaatatcg ggactaccgg catgcctatg ctctggcgcg tgcgctcgag aactatgcgg
     2521 ttctcggcga cgggcagtac ggctgtccgc gtgacaaggc tcgggaagcc ctgaggcgcg
     2581 ccatcggcgt actggaatcg gtccgcgagg aaggcctcga cagggcggag tggcacatgc
     2641 gcatggccta cggctaccag tacctgacgg gagaggagga aaaggccatt ccttatgcca
     2701 gaacctgggc tcagctcgat cctgaggatg aggatgcgcc gtgcgtcatc cgcgaatgtg
     2761 aaaaagcggt tgaggcccgt tgtaagaacg acgggaagcc cgttgacaga aagggcgtct
     2821 tcctcggctt cgtgctgctt gagaaggcgc attgggacaa gggcaggctg atcgccgatc
     2881 tgaaggccga atgggacatc gacgttcccg aggaggacaa ggacgacagg aaggacaacg
     2941 gggattcgct ggtgttcgag gcgggcgaca tgatcgccgc agtgagcctg atgcacggcc
     3001 cggtgccggg gggagaggcg gaggacaatg ccgaaaacaa ctacatgtgg cccgatgccg
     3061 tcaaagccgc acaaagccat gccgcccaca tcatggtaac cgtgctggga aaggagacgg
     3121 acgtacgtct gcgcgggcag ttcttcgtga agctcgctgc cgcctgctgt cgtcaaccgg
     3181 gtgtcctcgg cgtatacacg tcgggcgttg tctttgagcc gtcgttttat gccgacatgg
     3241 cgggcgtcat gaaggaaggc gaactcccgc tcaacaactg gatttggttc gggctccatc
     3301 gaagagaagg cggcgtctgc gggtatacct acggcatgga cgtcttcggc aaggaggagc
     3361 tggaggtgct cgatgcggat gccgatccgc tggcggtcag gaaatttctt gtcagtctgg
     3421 cgggctatgt gatcggttcg gacgtcactc tcaatgatgg cgagacgatc ggcttttccc
     3481 aaggcgacag gcatcgcatc acccgcagtc cgggcgtggc gcttccggac caaccgacgt
     3541 tcaaggtgtc cgtcgagcag aatgagattc tgctcgacga ggccgctcct catctggaga
     3601 gcattcgtga gaagtgtctc cctgtcgacg aactcaatgc cttcagtcat ctggcgattt
     3661 atctgcgctg gtgcatcgag cacgacctca tgagcgagtc attctgcaga cgctggcccg
     3721 atattgttga ggccgtgagg acgaatccgg ccgaggcgtg cctgcgcgaa tggctccgag
     3781 acaagctcaa gggcgaactg catgcttcaa tcttcaacga gcgcggcgcg gactttgcct
     3841 gctactacta cggtgccctt cacgagtaca cgccgcacta tacgatggat gtcgacatcc
     3901 atgccgtttc cagaataggc gtggagagga acttttcgga tgagattcag gacgaagcgt
     3961 atctgttcct tccgtttgat gaagactatt atcgagaaat ggcccggatc atcgagcggc
     4021 ggtacgagaa ttgggccgga cagcgctttg acgactcgac gcttgcgcct tccgaaacgg
     4081 ccgaggcgct gatggcgtat ctcgactgcg agtgcaccta tttcccgtcg atggcggacg
     4141 atgatccgat catgtgcgct ttcgagtatg ccgggaaaaa tgcagcgcac gaaggattcg
     4201 ttcccatgct cattgcagtc gacgaaacac tctgggaatg cctcatgctc aattccgatc
     4261 cggctcatga gggagatgac gatcatgcct ttgatccgga tgcggtagcc gcctaccggc
     4321 aatcgcagct cgccgcaccc ctgcccgacg gcaggatggt tctggaggac ggcatggcag
     4381 agcgccgcgc agaagcggag gacgattcca aggattggga cacggaagtc gtcggccgca
     4441 tggagggcgg cttcaacaac catcggttct ccggatattg gaacagcgag acggacatga
     4501 cgcatccggt catcctcgcg agaattccgg tccggcatcc ttgggaggtg tttgcccatc
     4561 tgccgttcgg caactggaat gactgtccgg atacgccgca gctcatggcg gtcgccaggt
     4621 actggttcga aaaattcggt gccgtgccag ccgtcatgac tcatgacgag ctcgacttcg
     4681 cgcttccgaa accgatcggc cgggagcttg ccgtgcagac tgcacttgag cagtatgcgt
     4741 tctgtcccga catggatcag aatcatgagt ccgtcggggc actcgcggat acgctccggc
     4801 agtccaaaat ctggtatttc tggtgggatt agctgtatat ctgcggacaa aggagggcgc
     4861 gtcatgaagg tccggcaatg ggcatggctt ctactcgcgg gggcggtcgc tccgtttgtc
     4921 ttcgacaatg gccccgatcc cgtctccgac gggctctatc gagtcgtgga tgaccacggt
     4981 cgcatcggct atgccgatgc atacggcgtc gttcgcatcg cgccccgatt tgcctttggt
     5041 ctgccttttg cgaacggcgt tgccagggtg accttgagcg gtcatcctgc ggcagtgccc
     5101 gggtcggaag gagaatatca tcgatgggag gacggcgact ggttctgcat taacaagtcc
     5161 ggctcaatcg tcaaatgccc gtcgtcatcg gatcatggga gccggccgtg aaagcctttg
     5221 tcgagaaact gttcgtcgaa tggaacgacg tcatatgcct tctgttcgga agcctctgca
     5281 tcctcggtgc gatccgagac tgggattggc tgtgcgatcc gacgggcaag cctcatgcgc
     5341 cgcttctgac gcgcgggctg ctccgtgcgg tcttcctcat gctcggaacc attctggtcg
     5401 tctgcagcgt gtggcttcta ctcaaaaggc agaattgagg agggacgatg aacttcaaca
     5461 gggaaacctg catgaggctg gctgccggca ccgttctttg cggcggtgcc gcgttgggcg
     5521 gcctcctttg gtggcagctt caggcgttcg accggatgac ggacagccgc ctcgagcgcg
     5581 gcatgggttt gttcgcgctt gagcttgagc gccgggacgg cggtcttctg cagcgcgatc
     5641 tggtgctgcg cctgggattt cgcacggaag acggcctgac cgaatccttt ctggttatga
     5701 acggtcgctt cagaccgggg ctccggcctt cggtcagctt cgagcccgta gcgaattcgg
     5761 atccggatgc tgcgatactc gtcaaggctg atcctcggat tcggttcgtc ttcagtccct
     5821 tgatgagtcc ggaagaggct gaggttgtct ggcgcggtgc gtcggatgaa aaggagacga
     5881 tcggcgacgg ccgtgtcacg gctgacatcg gctttgatcg cgcgcgccgt gcattgacgg
     5941 ggctgactgt ctccggacgg ctgggggcct ccgaaagccg atgggagggt ggtcatgtca
     6001 agagtgctga gaagaccttc gagtacgtct atgcagagtc gcccctcaag tttgcctttt
     6061 cctattcgtc cgcaggtgat tttgtcaagg gtgagctcat gccgctcggc atggggccct
     6121 tgagctacag cctcacggtg acgcctgagg aggaggcagg ccgcctgcgt aatgcgaccg
     6181 acttcaagtt cgacatccgg gacgtcaaca tcaacgattc ggagctgccg gttttcgacc
     6241 tgcggtttga tgccggcctg tacacgcctc cgaatgtctg gctg
//
LOCUS       DBMDTI010000108         6266 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3172_length_6266_cov_2.437307, whole genome shotgun sequence.
ACCESSION   DBMDTI010000108 DBMDTI010000000
VERSION     DBMDTI010000108.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6266)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3172_length_6266_cov_2.437307"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="ACZPB6_06230"
     CDS             <1..213
                     /locus_tag="ACZPB6_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="with YejAEF is involved in resistance to microcin
                     C; Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin ABC transporter ATP-binding protein"
                     /protein_id="HIR1184098.1"
                     /translation="MELLAELKPDHAGWLFISHDLKAVARLCDRVAVLSEGRIVEVMD
                     AARMGSPSSEAGRRFVEAAAAALPRA"
     gene            477..1997
                     /locus_tag="ACZPB6_06235"
     CDS             477..1997
                     /locus_tag="ACZPB6_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HIR1184099.1"
                     /translation="MTNQSRRSFLQGSIVGAAALASASVVQAAPRSKVPAKWDETVDV
                     IVVGSGFAGLAAAIEARKAGAKVVVLEKMPTPGGNSIINGGILTATGCPQQLKHGIKD
                     SVDLLAQDILVAGAYLNDPAKVRVLAEHALSNYEWTVKELGVEYNLDAIGQEGGHSVP
                     RYVFTKNGSGSGIVRKELDKCKELGVEVRLRAYVEHIIRDDKGRVVGLEVREGYRFPK
                     KDSGKVKFIAARKGVVLCYGGFSADVKFRQMHDPKLTDKFSTTNQPGATGELWRETYN
                     IGCAQIQNDWIQCGPWSNPKEKGMGIGWTFTQSAAAEYGLWVNTKGQRFVNELANRKV
                     RADAIFAEEAKGLKAVAIANQYGADKLEAARPGIMKKLVEPNIVSKYDTLEALAKDFN
                     IPLDELKKTLAQVNESAKTKNDKPFGRYINNEFKPLTEGPWYAAITSPKVHHCMGGLL
                     TNMKGQVLDIMDQEPIPGLYAAGEATGGIHGAVRLGSCAILDCLVNGRICGQEVAK"
     gene            2157..3632
                     /locus_tag="ACZPB6_06240"
     CDS             2157..3632
                     /locus_tag="ACZPB6_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HIR1184100.1"
                     /translation="MTEPLSRLPTLSRRTLLAGAAAAGLTAGMPPVRAAGLRWDGEAE
                     IVIVGSGGAGVSAALAARECTKGRIVILEKLGVTGGSTRFSCGYFNAVDPERQRAQGI
                     EDSLERHIADTNASGRGRGDPELVRILCSEALDALHWVEGYGVKYEPFCSQIYGGLFP
                     RSHLPKLSDPQASYIDVLLDACRSRGIEILTSSPVLSLVLNEDGSAAGVVWRDASGTR
                     CTLRASRAVVLASGGFSANAELCRLHDPRLGDLDTTNPAGSTGEVMLAAREAGAYLTG
                     CDWIECIPLHVHYARFAILVERCIFVDQQGRRFVREDDRRDILRDTILSLPSRVGFVI
                     VDHDGFESNPPSFRRELHEGLERQEVYRAQTLEEMAQLLRLPVNNFVETVNRYNGFVR
                     SGRDEDFGRGVESMIHEIRKPPFWVCRASMSRHHIMGGVSIDARARVLDWDSKPVGRL
                     YAAGEVAGGVHGASRLGGNAIADVHVFGRIAGREAASEVPA"
     gene            3629..4999
                     /locus_tag="ACZPB6_06245"
     CDS             3629..4999
                     /locus_tag="ACZPB6_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1184101.1"
                     /translation="MRRTDAQQGFSSAAADADVIVVGCGLAGLSAALAAAQRRARVVL
                     FDSSSDLFATNDVADRLYFNAVDPERQGDLGITDSPELFYRQTLAAGGGRADPALVKI
                     LCYQAYIALKWLEGFGVGFENKVRQIPGGVYPRTCVASDYRAGRLAMMNAALDAGVTV
                     LQGLTFRELVLDAQGRVTGVSAEDADGRRRILRAPATVLATGGFASDAALCARHDRRL
                     GALFDMRGGRASGLGLRAAVKAGAYLVGTDYFSLTMGTAGRAGFAVCPFHPLRFILVD
                     ASGGRVVNEQDREAVVEALLDAPGNVLWLAASFSDVARLPEVRRGRIEALLAEGTARR
                     VTGSEDMAGMEGAPSRASIVDAVRTYGEGPGDRLGKSWRVRIDDNDMLVMPVTLGRSA
                     TLGGVRIDGGGRVLTASGEHVEGLFAAGEITGGVHGMRCVPGNLSLESVVFGREAGFA
                     AAGRSA"
     gene            complement(5024..5227)
                     /locus_tag="ACZPB6_06250"
     CDS             complement(5024..5227)
                     /locus_tag="ACZPB6_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012422.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1184102.1"
                     /translation="MKDLATLTPREREVAGLVAEGLLNKVIAERLGIAEKTVQIHRGQ
                     VCRKLKVRSAVEISRILDQAEGR"
     gene            complement(5338..>6266)
                     /gene="iscB"
                     /locus_tag="ACZPB6_06255"
     CDS             complement(5338..>6266)
                     /gene="iscB"
                     /locus_tag="ACZPB6_06255"
                     /inference="COORDINATES: protein motif:HMM:NF040563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease IscB"
                     /protein_id="HIR1184103.1"
                     /translation="KGWLAPSVENRIQAHMSRIDAVCRLLPVTKIVIETASFDIQKIK
                     DPSVEGTDYQQGDQLGFWNVREYVLFRDGHVCQHCHGRSKDKILNVHHLESRQTGGDA
                     PNNLITLCETCHKAYHAGKIKLKVKRGQSFRAEAFMGIMRWTLLDRVRKAHPGLPVEN
                     TYGYLTKHTRITHGLPKTHCVDAYCIAGNLKAVRRGVYLHQRQMRKHNRQIHKFTVLS
                     KRLEDGTKIGYRKLNQSPYLVHDFRLFDKVRCLGQIGFIFGRRSSGYFDVRRLDGVKL
                     SPSISWRKLTLLEKRSTYLTELRKQDGASSPV"
BASE COUNT         1113 a         1988 c         2017 g         1148 t
ORIGIN      
        1 atggagcttc ttgccgaact caagcccgat catgccggct ggctcttcat ctcgcacgac
       61 ctcaaggccg ttgcaaggct ctgcgacagg gtcgccgtgc tctccgaagg ccgcatcgtc
      121 gaggtgatgg acgccgcacg catgggcagc ccctcgagcg aggcgggcag gcgcttcgtc
      181 gaggccgccg cggcggcgct ccctcgggcg taaacggtga tgtgagaggt ctccgacggc
      241 gcctgcggac ggcgtggacc tcctaggaac ataggatgtt catcctatgc cgtccggacg
      301 ccctgaacgg gatcctgccc cgaaggacag tcgtttcggg cggttttcgg gcgttttcaa
      361 gctcagatga agatcctctt ttgtttcggg acgagcctcc ttagactgat tctcaagcgg
      421 tgcgccggct gacctcggtg catccgctct ttgaccaaga taaggagaag tacatcatga
      481 ccaaccagtc ccgtcgttcc ttcctgcagg gctccatcgt cggcgccgcc gcgcttgcct
      541 ccgcttccgt cgttcaggcc gccccgcgct cgaaggttcc cgccaagtgg gatgaaaccg
      601 tcgacgtgat cgtcgtcggc tccggctttg ccggtcttgc cgccgccatt gaagcccgca
      661 aggcgggcgc caaggtcgtc gttctcgaga agatgccgac cccgggcggc aactccatca
      721 tcaatggcgg cattctgacc gccacgggct gcccgcaaca gctcaagcac ggcatcaagg
      781 actccgtcga cctgctcgct caggacatcc tcgttgccgg cgcctacctc aacgaccccg
      841 cgaaggttcg cgtcctcgcc gaacatgccc tctccaacta tgaatggacg gtgaaggaac
      901 tcggcgtcga atacaacctc gacgcgatcg gtcaggaagg cggccactcc gttccgcgct
      961 atgtcttcac gaagaacggc tccggttcgg gcatcgtgcg caaggaactc gacaagtgca
     1021 aggaactcgg cgttgaggtc cgcctgcgtg cctacgtcga acacatcatc cgcgacgaca
     1081 agggccgcgt ggtcggtctc gaagtccgag aaggctatcg cttcccgaag aaagactccg
     1141 gcaaggtgaa gttcatcgcc gccaggaagg gcgtcgttct ctgctacggc ggcttctcgg
     1201 ccgacgtcaa gttccgccag atgcacgacc cgaagctcac ggacaagttc agcacgacga
     1261 accagccggg cgccacgggc gaactctggc gtgaaaccta caacatcggc tgcgcccaga
     1321 tccagaacga ctggatccag tgcggtccgt ggtccaaccc gaaggaaaag ggcatgggca
     1381 tcggctggac gttcacgcag tccgctgcgg ctgaatacgg cctgtgggtc aacacgaagg
     1441 gccagcgttt cgtcaacgag cttgcaaacc gcaaggtccg cgccgacgcc atcttcgctg
     1501 aagaagccaa gggcctcaag gccgtcgcca tcgccaacca gtacggcgcc gacaagctcg
     1561 aagccgcacg tccgggcatc atgaagaagc tcgtcgaacc gaacatcgtc agcaagtacg
     1621 acacgctcga agcccttgcc aaggacttca atatcccgct cgacgaactc aagaagacgc
     1681 tcgcccaggt gaacgaatcc gcgaagacca agaacgacaa gccgttcggc cgctacatca
     1741 acaacgagtt caagccgctc accgaaggtc cgtggtacgc agccatcacg agcccgaagg
     1801 tccatcactg catgggcggt ctgctcacca acatgaaggg ccaggtcctc gacatcatgg
     1861 atcaggaacc gattccgggt ctctatgccg ccggcgaagc cacgggcggc atccacggcg
     1921 ccgtgcgtct gggctcctgc gccatcctcg actgccttgt caacggccgc atctgcggtc
     1981 aggaagtcgc caagtaacct ctcctcctga agtctcggcg tgagacggcg ggcggccttc
     2041 gggccgcccg tcttcgtttc gggctccgcg ccccgcacgt aggcattttc ccgagactgc
     2101 aggcacggca tgttcttata gactagagtg cagcaccttt ttcgaggcag acacccatga
     2161 ccgaacctct ctcccggctt ccgacactat cccgccgcac gcttcttgcg ggcgcggcgg
     2221 ccgcaggcct cacggcgggc atgccgcccg tgcgggccgc agggcttcgc tgggacggcg
     2281 aagcggagat cgtgatcgtg ggaagcggcg gcgcgggcgt ttccgcagcg ctcgccgcaa
     2341 gggagtgcac gaagggacgc atcgtcattc tcgagaagct cggcgtcacg ggcggaagca
     2401 cgcgcttttc atgcggctat ttcaatgcgg tcgaccccga aagacagcgg gcccagggca
     2461 tcgaggattc gctcgagcgg cacatcgccg acacgaacgc ctcaggccgc gggcgcgggg
     2521 accccgaact cgtcaggatc ctctgctcgg aggcgctcga cgcgctccac tgggtcgagg
     2581 gctacggcgt caagtacgag cccttctgct cgcagatcta cggcggcctc tttccgagaa
     2641 gccacctgcc gaagctctcc gacccgcagg cgagctacat cgacgtgctg ctcgacgcct
     2701 gcagaagccg cggcatcgag atcctgactt caagccccgt cctctccctc gtgctcaacg
     2761 aggacggttc ggccgcagga gtcgtatggc gcgacgcttc gggcacgagg tgtacgctca
     2821 gggcctcgcg ggccgtcgtg ctggcttcgg gcgggttttc ggccaacgcc gagctctgcc
     2881 gcctgcatga tccgaggctc ggagaccttg atacgaccaa tcccgcgggc tcgaccggcg
     2941 aggtgatgct cgccgcccgc gaggcgggcg cctatctgac gggctgcgac tggatcgagt
     3001 gcattccgct tcacgtccat tacgcgcgct ttgcaattct cgtcgagcgc tgcatcttcg
     3061 tcgaccagca gggacgccgc ttcgtgcgtg aggacgacag gcgcgacatc ctgcgcgaca
     3121 cgatcctttc gttgccctcg cgcgtcggct tcgtgatcgt cgaccacgac ggattcgaga
     3181 gcaatccgcc gtccttcagg cgcgagctcc acgagggact cgagcgacag gaggtctaca
     3241 gggcccagac gctcgaggag atggcgcagc tgctgaggct tccggtcaac aacttcgtcg
     3301 agaccgtgaa ccgctataac ggattcgtga ggtcgggcag ggacgaggac ttcggtcgag
     3361 gcgtcgagag catgatccat gaaatccgca aaccgccgtt ctgggtctgc agggccagta
     3421 tgagccgcca tcacatcatg ggcggcgttt cgatcgatgc aagggcgcgg gtgctcgact
     3481 gggattcgaa gcccgtcggc cggctctatg ccgcaggcga ggtcgcgggt ggcgtgcacg
     3541 gcgcaagccg tctcggcggc aacgcgatcg ccgacgttca cgtcttcgga cgcattgcag
     3601 gccgggaggc cgcctcggag gtgccggcat gagaagaacg gacgcacaac aggggttttc
     3661 gtccgctgcg gcggacgcgg acgtcatcgt cgtaggctgc ggccttgcgg gcctctcagc
     3721 cgctctggcc gcagcccaga ggcgtgcccg ggtcgtgctc ttcgacagct cgagcgatct
     3781 gttcgccacg aacgacgtgg ccgacaggct ctacttcaac gccgtcgacc cggagcgtca
     3841 gggcgacctg ggcatcacgg acagtccgga actcttctac cgccagacgc tggctgcggg
     3901 cggcgggcgc gccgatcccg cgctcgtcaa gatcctctgc tatcaagcct atattgcact
     3961 caagtggctc gagggcttcg gcgtcggatt cgagaacaag gtccgccaga ttccgggcgg
     4021 cgtctatccg agaacttgcg tcgcctccga ctacagggcg ggacgtctcg cgatgatgaa
     4081 cgctgctctg gatgcgggcg tgacggtcct gcagggactc accttcaggg aactcgtgct
     4141 tgatgcgcag ggacgcgtca cgggcgtttc ggccgaggat gcggacggac ggcgccgcat
     4201 cctcagggcg cccgcgacgg tgctcgccac gggcggcttt gcgtccgatg cggcgctctg
     4261 cgcaaggcat gacaggcgcc ttggcgcgct tttcgacatg cgcgggggac gcgcctcggg
     4321 gctcggcctc agggcagcgg tgaaggcggg cgcctacctc gtcggcacgg actacttttc
     4381 gctcaccatg ggcacggccg gccgcgcggg ttttgcggtg tgtcccttcc atccgctgcg
     4441 tttcatcctc gtcgacgcct cgggtggacg cgtggtcaac gagcaggaca gggaggccgt
     4501 cgtcgaggcg ctcctcgatg cgccgggcaa cgtgctgtgg cttgcggctt ccttctcgga
     4561 cgtcgcacgg ctgcccgagg ttcgccgcgg ccggatcgag gcgctgcttg ccgaaggaac
     4621 ggcaaggcgc gtgacgggct cggaagacat ggcgggcatg gagggcgccc cgtcgcgagc
     4681 ttcgatcgtc gacgccgtga gaacgtacgg cgaagggccc ggagaccgtc tgggcaagag
     4741 ctggcgcgtg cgcatcgatg acaacgacat gctggtgatg cccgtcacgc tcggtcgatc
     4801 ggcgacgctc ggaggcgtga ggatcgacgg gggcgggcgc gtgctgaccg cctcgggcga
     4861 gcacgtcgag gggctcttcg cggcaggcga gatcacgggc ggcgtgcacg gcatgcgctg
     4921 cgtgcccgga aacctttcgc tcgagtcggt cgtcttcggc cgcgaggcgg gctttgctgc
     4981 ggcgggccgc agcgcctgac ggcaggaaga atgcgggccg gcctcagcgg ccttcggcct
     5041 gatccagaat gcgggagatc tcgactgcgg agcggacctt gagcttgcgg cagacctgcc
     5101 cgcggtggat ctggacggtc ttttccgcaa tgccgaggcg ttcggcgatc accttgttga
     5161 ggaggccttc ggcgacaagg cccgcaactt cgcgctcgcg cggcgtgagc gtggcgaggt
     5221 ccttcatggc ggcgatgtga ctcgtgtggt gcgagcgccg ctcccaatcg agcgcgatgg
     5281 ccttctcgac gctcgcgagc agcttttcat agaaattcgg cgcggaaacc tctgtcttca
     5341 gacaggggag gaagcgccgt cctgctttct aagttcggtt aaataggtac ttctcttttc
     5401 gagaagcgtg agtttcctcc agctgatact aggggaaagc tttacgccgt ccagcctccg
     5461 tacatcgaag tagccggacg atcttcgccc gaagatgaag ccgatttgcc caagacatct
     5521 caccttgtca aagagcctga agtcatggac gagataaggt gactggttga gcttgcggta
     5581 tccgatcttc gtaccgtcct caagtctctt cgagagcacc gtgaacttat gtatctgacg
     5641 gttgtgcttg cgcatctgcc gctgatggag gtaaacgcct cgtcgaacgg ccttgaggtt
     5701 tcccgcgatg cagtaggcat cgacgcaatg cgtcttgggg agtccatggg tgatgcgcgt
     5761 gtgcttcgtt agatagccgt aggtgttctc cacaggcaag ccaggatgcg ccttgcgaac
     5821 gcgatcgagc aatgtccaac gcatgatgcc catgaaggct tccgccctga acgattgacc
     5881 gcgcttgacc ttcaacttga tcttccctgc gtgataggcc ttgtggcagg tctcgcagag
     5941 cgttatcagg ttgttgggcg catccccgcc ggtttgacgg ctttcgaggt ggtgcacgtt
     6001 gaggatcttg tccttcgaac gaccgtgaca gtgttggcaa acgtgaccgt ccctaaagag
     6061 aacgtattca cgcacgttcc aaaagccgag ctgatcgccc tgctgatagt ccgtgccttc
     6121 aacagacgga tccttgatct tctgaatgtc gaacgatgcc gtctcaatca cgatcttggt
     6181 gacgggaagc agtctgcaga ccgcatcgat gcgcgacatg tgcgcctgaa tgcggttttc
     6241 aacggacggt gcaagccatc ccttgt
//
LOCUS       DBMDTI010000109         6232 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3199_length_6232_cov_2.408416, whole genome shotgun sequence.
ACCESSION   DBMDTI010000109 DBMDTI010000000
VERSION     DBMDTI010000109.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3199_length_6232_cov_2.408416"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="ACZPB6_06260"
     CDS             complement(<1..292)
                     /locus_tag="ACZPB6_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184104.1"
                     /translation="MTGILNTTSLSRAAAAALSALLLSGCAALSGGGSEAIPADAMKR
                     PVDLSVVCVLVNEDVKSEPLVKSLETGVRSFGSEPRLLMPGDGPQACSFVVTY"
     gene            558..1187
                     /locus_tag="ACZPB6_06265"
     CDS             558..1187
                     /locus_tag="ACZPB6_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1184105.1"
                     /translation="MHDPAKIDKPLIRVIDDDDAMRNSWAFLIEGEGWDVKTYADAIT
                     FLSSNDYIRPGCLLLDVRMPHMSGLELQLKLREAGCDLPIIFISGHGDIDMAVNTLKA
                     GAIDFLQKPVDDQRLLKTIENAVSHNLAQRRGKREVTDFRNRLESLTQREREVIRMVA
                     QGYSNKEVAREYGISEKTVQVHRGSAYRKLDLHNAAEIARLLMLTGDPL"
     gene            1448..3091
                     /locus_tag="ACZPB6_06270"
     CDS             1448..3091
                     /locus_tag="ACZPB6_06270"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR1184106.1"
                     /translation="MLSDIEIAQATKLIPIDQLAHEAGLTDAEFEPYGRDKAKVELDA
                     SRPDTAKLILVTATSGMPAGSGKTTTSIALAQGLKQLGKKAVLALREPSLGPVFGMKG
                     GAAGGGYSQVLPMEAINLHFTGDFHAITSANNLLAALIDNARHQGQVELKEIFWRRVM
                     DVNDRMLRNIVTGLGGSANGIPTEAGFDITAASELMAVLCLATDLDDLRARIDRIVVG
                     TRRDGSAVTVQELGVGGALVALLKDAIKPNLVQSIEGNLAFVHGGPFANIAHGCNSVA
                     ATRAAMKLGDYAITEAGFGSDLGAEKFYNIKCRAAGLNPAAVVLVTSTKALKWHGGVP
                     LPEIGKPNAEALKKGLCNLDAHVENLKRFGPNIVVSINHFHTDLDEELDIIRNRCAEL
                     GVRVALSDGFALGGKGAVDVARAVIEAAEDVHPLNFTYEADAPVMEKVEKIAKTIYGA
                     AAVELAPAAAKDLKRLQDLGFDRLPVCIAKTPFSFSHDPKLLGAPKGFTLPVQRLILN
                     AGAGFVVVTTGAIMRMPGLPKVPAAMSIDVKDGKITGLA"
     gene            complement(3283..3397)
                     /gene="rrf"
                     /locus_tag="ACZPB6_06275"
     rRNA            complement(3283..3397)
                     /gene="rrf"
                     /locus_tag="ACZPB6_06275"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            3555..5294
                     /locus_tag="ACZPB6_06280"
     CDS             3555..5294
                     /locus_tag="ACZPB6_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HIR1184107.1"
                     /translation="MTLNKSLVCLAIASLSIPAMAAPVTTEGAGVGKHGDVIAAVTFD
                     GGRIQAIDIRKSNENPILAGKVFTEMKDAMIKHNTADIDAVTGATVSSDALRNAVKEA
                     AAKAGVTLAGPVALLKRAPKVPETNVYDVVVIGAGGAGFSAAITASDAGAKVVLLEKM
                     PNVGGNSLVSGAEMAAAGNWVQKKLGIEGDSVELHYQDTMKGGDMKGDPAVVRTMVEN
                     ALPAAEWCRDVIGVEFQEDNLFFFGGHSKKRSLIPKGATGLDFITKFSAAAEKRGIPI
                     ITNMKAEELVRDASGRVTGVKATMDGKSYTFSARKGVVIATGGFAANVAMRTEANPFY
                     GDGFKTTNMPGAMGEGITMAKNIGAQVVNMGLIQTYPMCDPNSGAIELIDDARFEGAI
                     LVNQEGKRFVEELQRRDVMSKAILEQTGKYCYAIFNGEIEKRSHAITHHQDEVEVFTK
                     TGILHKADTIEGIADFFKIPVDNLKATIARVNEFARTGKDLDFNYRSRFVDLSTGPYW
                     IYRGVPSVHHTMGGLKINPKAKVLDANDKPIPGLWAAGEVTGCTHGTNRLGSNAYTDI
                     IVFGRIAGAEVAK"
     gene            5397..6017
                     /locus_tag="ACZPB6_06285"
     CDS             5397..6017
                     /locus_tag="ACZPB6_06285"
                     /inference="COORDINATES: protein motif:HMM:NF037679.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1184108.1"
                     /translation="MATKGEQSRQRILEASKSIILESGIGKLSHGAIAEVLDISKSAI
                     HWHFPTKDDLLKALIDDYLEHLQAEEKRHETPFIASGLSPEDAVLPAMRLWYLDFREN
                     RAGWVGLGSQLLSLSSRHPALVEPIRAWYKSLYDRIGRSSADTVRGYVAMMVFDGFFN
                     SSKMGVMTLSDEEMDAMQMHVLREAYAARPDMLEVIEAVIGSGERM"
BASE COUNT         1260 a         2094 c         1788 g         1090 t
ORIGIN      
        1 cgtacgtcac gacgaaggag caggcctgcg ggccgtcgcc cggcatgagc agcctcggct
       61 cgctgccgaa gctcctcacg cccgtctcaa gcgacttgac gagcggttcg ctcttgacgt
      121 cctcgttaac gagcacgcag acgacggaga ggtcgacggg gcgcttcatc gcatcggcgg
      181 gaatggcctc ggaaccgccg cccgaaagcg cggcgcagcc cgagagcagg agcgccgaca
      241 gggcggccgc cgcagcgcgg gataatgagg ttgtgttgag aatgccggtc atggacgtcg
      301 tgtgttgtct aaaggattgc ccatactagc agaggaaggc gcatcctgcg tcgccgttgc
      361 gttttctcgg ccagattctg tcgccccaac acctcatttc gggtaagatg aaatctgcgg
      421 gacgggagcg caacctccat ccgcatcccg cttcatttca tgattcaata aaaatggccg
      481 gacgcgcgcc gccttcgcct cctccccttg gacgagccgg acgctccaag cacaaccata
      541 ataactagga tttcaccgtg cacgatcctg cgaaaatcga caagcccctg atccgcgtca
      601 tcgacgatga cgacgcaatg cgcaactcct gggccttcct cattgaaggc gaaggctggg
      661 acgtcaagac ctatgccgac gccatcacgt tcctttcctc gaacgactac atccgccccg
      721 gctgtctcct cctcgacgtc cgcatgccgc acatgtccgg ccttgagctt cagctcaagc
      781 tgagggaagc cggctgcgac ctgccgatca tcttcatttc cggccacggc gacatcgaca
      841 tggccgtcaa caccctgaag gcgggcgcca tcgacttcct tcagaagccc gtcgacgacc
      901 agcgcctcct caagaccatt gaaaacgccg tctcgcacaa cctcgcccag cgccgcggca
      961 agcgcgaagt caccgacttc cgcaaccgtc tcgagtcgct cacgcagcgc gagcgcgaag
     1021 tcatccgcat ggtcgcccag ggctactcca acaaagaggt tgcccgcgaa tacggcattt
     1081 cggaaaagac cgtccaggtc caccgaggct ccgcctaccg caagcttgac ctgcacaacg
     1141 ccgctgaaat cgcacgtctt ctcatgctga cgggcgatcc gctttaaagc gacagctctc
     1201 accaaatccc gtccggaaca aggcgttcgg acgggatttt tgcatcctgc ccgcacgctc
     1261 ggaaatattt acggttttcg tccacccgtc ccagcttttt gaggcatact ctctcaatcc
     1321 cgccatctgg acggcaggag acgctccgtt gaaggctccc tcagggtcga gcgccggagg
     1381 ctcccggacc gtgccgcagg ccatcggttt tcagtttttt caggcattta cctttttggg
     1441 agatcccatg ctcagcgata ttgaaattgc tcaggcaacc aagctcattc cgattgacca
     1501 gcttgcccac gaggccggcc tcaccgacgc cgagttcgag ccgtacggcc gcgacaaagc
     1561 caaggtcgag ctcgacgcct cccgtcccga cacggccaag ctcatcctcg tgacggccac
     1621 gtccggcatg cccgcaggct ccggcaagac caccacgtcg atcgccctcg cccagggtct
     1681 caagcagctc ggcaagaagg ccgtactcgc ccttcgcgaa ccgtccctcg gcccggtctt
     1741 cggcatgaag ggcggcgctg ccggcggcgg ctactcgcag gtccttccga tggaagccat
     1801 caacctgcac ttcacgggcg acttccacgc catcacctcc gccaacaacc tcctcgccgc
     1861 tctgatcgac aacgcccgcc accagggtca ggtcgagctc aaggaaatct tctggcgccg
     1921 cgtcatggac gtgaacgacc gcatgctccg caacatcgtc acgggtctcg gcggctccgc
     1981 caacggcatc ccgacggaag cgggcttcga catcacggcc gcctccgaac tcatggccgt
     2041 cctttgcctc gccacggacc ttgacgacct gcgcgcccgc atcgaccgca tcgtagtcgg
     2101 cacgcgccgc gacggttccg ccgtcacggt tcaggaactc ggcgtgggcg gcgccctcgt
     2161 cgcgctcctc aaggacgcca tcaagccgaa cctcgtccag tccatcgaag gcaacctcgc
     2221 cttcgtgcac ggcggtccgt tcgccaacat cgcccacggc tgcaactccg tcgccgccac
     2281 gcgcgccgcg atgaagctcg gcgactacgc catcaccgaa gccggcttcg gctccgacct
     2341 cggcgccgaa aagttctaca acatcaagtg ccgtgcggcc ggtctcaacc ccgccgccgt
     2401 cgtgctcgtg acgtccacca aggccctcaa gtggcacggc ggcgtccccc tgcccgaaat
     2461 cggcaagccc aacgccgaag ccctcaagaa gggcctctgc aacctcgacg cccacgtcga
     2521 gaacctcaag cgcttcggtc cgaacatcgt ggtctccatc aaccacttcc acacggacct
     2581 tgacgaagaa ctcgacatca tccgcaaccg ctgcgccgaa ctcggcgtgc gcgtggccct
     2641 ctccgacggc ttcgcgctcg gcggcaaggg tgccgtcgac gttgcccgcg ccgtgatcga
     2701 ggctgccgag gacgtgcacc cgctcaactt cacgtacgag gccgacgccc ccgtcatgga
     2761 aaaggtcgag aagatcgcca agaccatcta cggcgctgcc gccgtcgaac tggctcctgc
     2821 cgccgccaag gatctcaagc gcctgcagga cctcggcttc gaccgcctcc cggtctgcat
     2881 cgccaagacc ccgttctcgt tctctcacga tcccaagctc ctcggcgctc cgaagggctt
     2941 cacgctgcct gtccaacgcc tcatcctcaa tgcgggcgca ggcttcgtcg tcgtgacgac
     3001 gggtgcgatc atgcgcatgc ccggcctgcc gaaggttccg gccgccatgt ccatcgacgt
     3061 caaggacggc aagatcaccg gtctcgcgtg atccgtctga tacgactggt tgaaacagca
     3121 tgacgaaggg cccatgcagc aatgcacggg ccctttgtct tgtcatgcca tcggctgtcg
     3181 gggcctcgag aacattcgag cgggcgccct gccgtctgaa aaacaaaagc ccgaaaagac
     3241 aaaaccccgg cttccttgga aaccggggtt ttgcaattgc gagcctgacg atgtcctact
     3301 ttcactggaa atacaaccaa ctatcatcgg cgataaggcg tttcactgtc ctgttcggaa
     3361 tgggaaggag tggtaccgcc tctctatggt catcaggcaa tggcttttgt ctgctttcgc
     3421 aaacgatttt gatgatttta tcataaaacc gagtgtctgt cgacggcata agtcccaatc
     3481 atacaaaact atgctcgggg tcagtttttg gctacattga cctcatgacc acaccaatcg
     3541 gagaggagcc gaccatgaca ctgaacaaat ctctcgtctg tcttgcaata gcgtcgcttt
     3601 ccattcccgc catggctgcg cccgtcacga ccgagggcgc gggcgtcggc aagcacggcg
     3661 acgtgatcgc cgcagtgacc tttgacggcg gcaggattca ggcgatcgac atcagaaaat
     3721 cgaacgagaa cccgatcctc gcgggcaagg tcttcaccga aatgaaggac gcgatgatca
     3781 agcacaacac ggccgacatc gatgccgtga cgggcgcgac cgtctcgtcg gacgccctca
     3841 ggaacgccgt gaaggaggct gcggccaagg cgggcgtgac gctcgcgggc ccggtggccc
     3901 ttctcaagcg cgcgccgaag gtgcctgaga cgaacgtcta cgacgtcgtg gtgatcggtg
     3961 cgggcggcgc gggcttctcg gccgccatca ccgcgagcga cgcgggcgcg aaggtcgtgc
     4021 ttctcgaaaa gatgcccaac gttggcggca actcgctcgt ttcgggcgcg gaaatggccg
     4081 ctgcgggcaa ctgggtccag aagaagctcg gcattgaagg cgacagcgtt gaactgcatt
     4141 atcaggacac catgaaaggc ggcgacatga agggcgatcc cgccgtcgtg cgcaccatgg
     4201 tcgagaatgc gcttcccgcc gccgaatggt gccgggacgt catcggcgtc gaattccagg
     4261 aagacaacct cttcttcttc ggcggccact ccaagaagcg ctcgctcatt ccgaagggcg
     4321 ccacgggcct cgacttcatc accaagttca gcgctgcggc cgagaagcgc ggcattccca
     4381 tcatcacgaa catgaaggcc gaggagctcg tgcgtgacgc ttcgggacgc gtcacgggcg
     4441 tcaaggccac gatggacggc aagagctata ccttcagcgc caggaagggc gtcgtgatcg
     4501 cgacgggcgg ctttgccgcg aacgttgcga tgcgcacgga ggccaacccg ttctacggcg
     4561 acggcttcaa gaccacgaac atgcccggcg ccatgggtga aggcatcacg atggcgaaga
     4621 acatcggcgc acaggtcgtc aacatgggcc tcatccagac ctatccgatg tgcgacccga
     4681 actcgggcgc catcgagctc atcgacgacg ctcgcttcga aggcgccatc ctcgtcaatc
     4741 aggaaggcaa gcgcttcgtc gaggaactcc agcgccgcga cgtgatgagc aaggccattc
     4801 tcgagcagac gggcaagtac tgctacgcga tcttcaacgg cgagatcgag aagcgctcgc
     4861 acgccatcac gcaccatcag gacgaggtcg aggtcttcac caagacgggc atcctgcaca
     4921 aggccgacac catcgagggc atcgccgact tcttcaagat cccggtcgac aacctcaagg
     4981 ccacgattgc gcgcgtcaac gaattcgcca ggacgggcaa ggacctggac ttcaactacc
     5041 gcagccgctt cgtcgacctc agcacgggac cgtactggat ctacagaggc gttccgagcg
     5101 tgcaccacac gatgggcggc ctgaagatca atccgaaggc caaagtgctc gacgccaacg
     5161 acaagccgat tcccggcctc tgggctgcgg gcgaagtgac gggctgcacg cacggcacga
     5221 accgcctcgg ctcgaacgcc tacaccgaca tcatcgtctt cggccgcatc gcgggtgcgg
     5281 aagtcgccaa ataatgacgt aggctgaagc ctggtcgact tatacttaag tggatctccg
     5341 ggcccgtcct ccgagtggag acgggcccgg attcgtttca tcgtcagggg attcacatgg
     5401 ccacgaaggg cgaacaaagc aggcagcgga ttctggaggc gtccaaaagc atcattctcg
     5461 agagcggcat cgggaagctg agccacggcg cgatcgccga ggtgctcgac atctccaaga
     5521 gcgccatcca ctggcacttc cccacgaagg acgatctgct caaggcgctc atcgacgact
     5581 atctcgagca tctacaggcc gaggagaagc gtcatgagac gcccttcatc gcctcgggcc
     5641 tcagtcccga ggacgccgtg ctgcccgcca tgcgcctctg gtatctggac ttcagggaga
     5701 accgcgccgg ctgggtcgga ctcggctcgc agcttctgag cctctcgtcg cgccatcccg
     5761 ccctcgtcga gcccatccgc gcatggtaca agtcgctcta cgacaggatc ggccgttcgt
     5821 cggccgacac cgtcaggggt tacgtcgcca tgatggtctt cgacggcttt ttcaactcgt
     5881 ccaagatggg cgtcatgacg ctttcggacg aggagatgga cgccatgcag atgcacgtgc
     5941 tgcgcgaggc ctatgcggcc cgccccgaca tgcttgaggt catcgaggcc gtgatcggca
     6001 gcggcgagcg catgtgattc cgagccggtc gaacgattcc tgacccgctc ggccgcactc
     6061 cggcgttttc tgacgggaag gccctgccgc aaggcggggc cttgcgcatt ttgctgctcg
     6121 ggaaaaacaa gaaccccggt ctctttcgaa accggggttc ttggaatggt gagcctgacg
     6181 atgtcctact ttcactggaa atacaaccaa ctatcatcgg cgataaggtg tt
//
LOCUS       DBMDTI010000110         6224 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3205_length_6224_cov_2.823616, whole genome shotgun sequence.
ACCESSION   DBMDTI010000110 DBMDTI010000000
VERSION     DBMDTI010000110.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3205_length_6224_cov_2.823616"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="ACZPB6_06290"
     CDS             <1..551
                     /locus_tag="ACZPB6_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR1184109.1"
                     /translation="VMKGYWNREEATKAIFTEDGWLRTGDVCTIYPDGNIRITGRIKE
                     IIVTSTGEKVPPADLESAITSDRLFSQCMVVGDDRPFIAAVAVVNPDEFKTVCGELGL
                     DPAKPESLQDPAFRKAALKRIKTATAGFPNYGVPRQVHCTLDAWTIDNGLLTPTLKMK
                     RNLIRQRYTAEINALYDTLVKR"
     gene            749..2596
                     /locus_tag="ACZPB6_06295"
     CDS             749..2596
                     /locus_tag="ACZPB6_06295"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR1184110.1"
                     /translation="MTNTRSFVDDFRTIPDMLRHTVAAWPEREAFRQYDYAERRWYSI
                     TWRTFHEQVMRWRRAFTHMGLVKGERVAMLLTNCIDAVTFDEAALCGGLVPTPLHAID
                     TAGSSAYILRDSNARFLVTTSRARWNAIAEAAGEEGLPDMQLVVFTNEQVEPGSVTHL
                     GGRDIVLSSLEGWLATGEGVSDDELPPEPCADDLAGFIYTSGTTGRPKGVMITHRNIV
                     SNVQQINKCYDFNEEDVFLSFLPFSHTFERTVAHYNSLANGGAMAFARNAAQIENDLR
                     EVRPTLMCSVPRIYEKIHQRLLHELAESSEEKRHQTAWALETGWRRFAEANHLPIELS
                     PRAQLDETVWPQLDVEIASEVREIFGGRLRATFAGGASLNYVVARFFCALGINILQLY
                     GLTETSPIVSFTRMKDNHPDCVGHPVPDCEVKLGANDELLVRGPQVMKGYWNRPLDTA
                     AAFEDGWFRTGDQADLSDGGRVRIKGRIKEIIVTSTGEKIPPVDVEFAIQEDRLFEQV
                     LVIGDNLPYVTCLVVVNPKLWPAFCADLDLDPEDPATLQSRDVQRQCLRRVRHACRAF
                     PQYGVPRAVAITREPWTVDNGLLTPTMKLRRPQIMTAHAKLIERLYASR"
     gene            2921..4387
                     /gene="guaB"
                     /locus_tag="ACZPB6_06300"
     CDS             2921..4387
                     /gene="guaB"
                     /locus_tag="ACZPB6_06300"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR1184111.1"
                     /translation="MRILQKALTFDDVLLVPAYSEVLPRDTQLATQLTRNLRINIPMV
                     SAVMDTVTEARLAIAIAQEGGIGFIHKNMSADQQAAEVLKVKRHEAGVVAEPITIGPD
                     MLVGDVIALAREKHISGLPVVDGKKVLGMVTNRDLRFETRMSVPVSEVMTPRERLVTV
                     KEGASLEEAKALMHAHRLERVLVVDDDFNLAGLMTVKDITKATDHPFAAKDASGKLLV
                     GAGVSVGGGTEERVEKLVDAGVDVIVVDTAHGHSQGVLNRVKWVKEHYPNLQVIGGNI
                     ATAAAALALVDAGADCVKVGIGPGSICTTRIVAGVGVPQISAISNVAEALRDTGVPCI
                     ADGGIRFSGDISKAIAAGANAVMMGGMFAGTDEAPGEVILYQGRSYKSYRGMGSLGAM
                     GKGSADRYFQDNNQGNIDKFVPEGIEGMVPYKGPLAQIIYQMIGGLRSSMGYCGCRTI
                     DEMRTRAQFVEITAAGWRESHVHDVKITKEAPNYRQEH"
     gene            4502..6067
                     /gene="guaA"
                     /locus_tag="ACZPB6_06305"
     CDS             4502..6067
                     /gene="guaA"
                     /locus_tag="ACZPB6_06305"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR1184112.1"
                     /translation="MSHDRILILDFGSQVTQLIARRVREAHVYCEVHPNDVSAEFIRE
                     YNPKGIILSGSHMSAYEESNDQASQAVFEAGVPVLGICYGMQTMAQQLGGRVESGTKR
                     EFGYAEVRAHGHTKLLEGIEDFRTEEGHGMLKVWMSHGDKVAELPPGFKVMCSTPSCP
                     IAGMCNEEKGFYAVQFHPEVTHTLKGREMLNRFVLDICKCSADWVMGDYVAEAVAQIR
                     EQVGDEEVILGLSGGVDSSVAAALIHRAIGKQLTCVFVDNGLLRKNEREQVRETFEKN
                     MGLKLIVVDAVDRFMNELAGVTDPEQKRKIIGRVFVEIFQEEASKLTAAKWLAQGTIY
                     PDVIESAGAKTKKAKTIKSHHNVGGLPDTLHLKLLEPLRSLFKDEVRELGVELGLPAE
                     MVYRHPFPGPGLGVRILGEVKREYADLLREADAIFIEELRNFDLYDKVSQAFVVFLPV
                     KSVGVMGDGRTYEWVVSLRCVETSDFMTAVWSHLPYELLGKVSNRIINEVRGINRVVY
                     DVSGKPPATIEWE"
BASE COUNT         1201 a         2226 c         1692 g         1105 t
ORIGIN      
        1 cagtcatgaa gggctactgg aaccgcgagg aggccaccaa ggccatcttc accgaggacg
       61 ggtggctgcg cacgggcgac gtctgcacga tctaccccga cggcaacatc cgcatcacgg
      121 gccgaatcaa ggagatcatc gtgacgagca ccggcgaaaa ggtgccgccc gccgacctcg
      181 agtccgccat cacctccgac cgcctcttct cgcagtgcat ggtcgtgggc gacgaccgtc
      241 cgttcatcgc cgccgtcgcg gtcgtcaatc ccgacgagtt caagacggtc tgcggcgagc
      301 tcggcctcga tcccgccaag cccgagtcgc ttcaggaccc cgccttccgc aaggccgccc
      361 tcaagcgcat caagaccgcc accgcgggct tcccgaacta cggcgtgccg cgtcaggtcc
      421 actgcacgct cgacgcgtgg acgatcgaca acggactcct cacgcccacg ctcaagatga
      481 agcgaaacct catccgccag cgctacacgg ccgaaatcaa cgcgctctac gacaccctcg
      541 tcaagcgctg atccccgcct tccgatgccg cccagacgcc ccgccgtcgg gcggcatttt
      601 tcagcctgag gggaactttt tacggatact agggtatacc ccgattactt tatccgcatc
      661 cgcaggccat ttttgaagaa aattgatccg ctgacccgcg gcgcccaagt gccgcggcaa
      721 cgagacacct ttcgcttgag agactcacgt gacgaacacc cgatcctttg tcgacgactt
      781 ccgcaccatt ccggacatgc tccgccacac ggtcgccgcc tggcccgaac gcgaggcgtt
      841 ccgccagtac gactatgcgg agcgccgctg gtactccatc acctggcgga cgttccacga
      901 acaggtgatg cgctggcgcc gcgccttcac gcacatgggc cttgtcaagg gcgagcgcgt
      961 cgcgatgctg ctcaccaact gcatcgacgc cgtcacgttc gacgaggcgg ccctgtgcgg
     1021 cggcctcgtg cccacgcccc ttcacgccat cgacaccgcg ggctcctccg cctacatcct
     1081 cagggactcc aacgcgcgct tcctcgtcac cacgtcgcgc gcacgctgga acgccatcgc
     1141 cgaggccgca ggcgaggagg gcctgcccga tatgcagctc gtcgtcttca cgaacgagca
     1201 ggtcgagccc ggctccgtca cgcacctggg cggcagggat atcgtccttt cgagcctcga
     1261 gggctggctt gccaccggcg agggcgtctc cgacgacgag ctcccgcccg agccctgcgc
     1321 cgacgacctc gcgggcttca tctacacctc gggcaccacg ggccgcccca agggcgtgat
     1381 gatcacgcac cgcaacatcg tctccaacgt ccagcagatc aacaagtgct acgactttaa
     1441 cgaggaggac gtcttcctct cgttcctgcc cttctcgcac acgttcgagc gcaccgtcgc
     1501 gcactacaac tcgctcgcca acggaggcgc catggccttc gcccgcaacg ccgcgcagat
     1561 cgagaacgac ctgcgcgaag taaggcccac gctcatgtgc tcggtgcccc gcatctacga
     1621 gaagatccac cagaggctcc tgcacgagct tgcagagagc tccgaggaga agcgccacca
     1681 gaccgcttgg gcgcttgaga ccggctggcg ccgcttcgcc gaggccaacc accttccgat
     1741 cgagctctcg ccccgcgcac agctcgacga aaccgtctgg ccccagctcg acgtcgagat
     1801 cgcgtccgaa gtccgtgaga tcttcggcgg ccgcctgcgc gccacgtttg cgggcggcgc
     1861 aagcctcaac tacgtcgtcg cacgcttctt ctgcgcgctc ggcatcaaca tcctgcagct
     1921 ctatggcctc accgaaacct cgcccatcgt ctccttcacc cgcatgaagg acaaccaccc
     1981 cgactgcgtg ggccatcccg tgcccgactg cgaagtgaag ctcggcgcca acgacgagct
     2041 cctcgtgcgc ggtccccagg tgatgaaggg ctactggaac cgtccgctcg acacggccgc
     2101 ggccttcgag gacggctggt tccgcacggg tgatcaggcc gacctctccg acggcggccg
     2161 cgtgcgcatc aaaggccgca tcaaggaaat catcgtcacg agcaccggtg agaagatccc
     2221 gcccgtcgac gtcgagttcg ccatccagga ggaccgtctc ttcgagcagg tcctcgtcat
     2281 cggcgacaac ctgccctacg tcacctgcct cgtagtcgtc aatcccaagc tctggcccgc
     2341 cttctgcgcc gacctcgacc tcgaccccga ggatcccgcc acgctccagt cccgcgacgt
     2401 ccagcgccag tgcctgcgcc gcgtgcgtca tgcctgtcgc gccttcccgc agtacggcgt
     2461 cccacgcgcc gtcgccatca cgcgcgagcc ctggaccgtc gacaacggcc tgctcacgcc
     2521 caccatgaag ctgcgccgcc cgcagatcat gacggcccac gccaagctca tcgaaaggct
     2581 ctacgcctcc cgctgagacc tgaggcacac cttcctagag gggcgtccga acaggacgcc
     2641 cctctctttc attgtgcggc agcggtttcg gggacgctcg aaaatatttc atccaaaatc
     2701 ccacaagccg caaaaaacgc gctacactac tgccgcaaat caatctccca attgcagaac
     2761 cggtcgagac cactcccgct tctgcagaag agcaggtcag cggatgccag atccctcctg
     2821 tactgcgggc gttcaatttc ctatttacgc gcgcatcttc gaccttcgca tacacgtcga
     2881 ggatacgcac gtatattttt ttttgtgctt agaggttgtt atgcgaatcc ttcagaaggc
     2941 tctcaccttc gacgacgttc ttctcgtccc cgcctattcc gaagtcctcc cccgcgatac
     3001 ccagctcgcc acgcagctga cgcgtaatct ccgtatcaac attccgatgg tttccgccgt
     3061 gatggacacg gttacggaag cccgtctcgc catcgccatc gctcaggaag gcggcatcgg
     3121 cttcattcac aagaacatgt ccgccgacca gcaggccgct gaagtcctta aggtcaagcg
     3181 tcatgaagcc ggcgttgttg ccgaaccgat caccatcggc cccgacatgc tcgtcggcga
     3241 cgtcatcgcc ctcgcccgcg aaaagcacat ctccggtctc ccggtcgttg acggcaagaa
     3301 ggttctcggc atggtcacca accgcgacct ccgcttcgaa acccgcatgt ccgttccggt
     3361 ctccgaagtc atgaccccgc gtgaacgcct ggtcacggtc aaggaaggcg cctccctcga
     3421 agaagccaag gccctcatgc acgcccaccg cctcgagcgc gttctcgtcg ttgacgacga
     3481 cttcaacctc gcaggcctca tgaccgtcaa ggacatcacc aaggccacgg accacccgtt
     3541 cgctgccaag gacgcctccg gcaagctgct cgtcggtgcc ggcgtctccg tcggcggcgg
     3601 cacggaagaa cgtgtcgaga agctcgttga cgccggtgtc gacgtcatcg tcgtcgacac
     3661 ggcccacggc cactcccagg gcgttctgaa ccgcgtcaag tgggtcaagg aacactatcc
     3721 caacctgcag gtcatcggcg gcaacatcgc cacggccgct gccgctctgg ctctcgttga
     3781 tgccggcgcc gactgcgtca aggtcggtat cggcccgggc tccatttgca cgacccgcat
     3841 cgtcgccggt gtcggcgttc cgcagatctc cgccatctcc aacgtcgccg aagctctccg
     3901 cgacacgggc gttccctgca tcgccgacgg cggcatccgc ttctccggcg acatctccaa
     3961 ggccatcgct gccggtgcca acgccgtcat gatgggcggc atgttcgccg gtacggacga
     4021 agccccgggt gaagtcatcc tctaccaggg ccgctcctac aagtcctacc gcggcatggg
     4081 ctccctcggt gccatgggca agggttccgc cgaccgctac ttccaggaca acaaccaggg
     4141 caacatcgac aagttcgttc cggaaggcat cgaaggcatg gtcccgtaca agggcccgct
     4201 cgcccagatc atctatcaga tgatcggcgg cctgcgctcc tccatgggct actgcggctg
     4261 ccgcacgatc gacgaaatgc gcacccgcgc ccagttcgtc gaaatcacgg ccgccggctg
     4321 gcgtgaatcc cacgtccatg acgtgaagat cacgaaggaa gctccgaact accgtcagga
     4381 acactaattc cgacggctga caggctctga cgtcaaggcg cggcggtttt tcccgccgcg
     4441 cctttcgcat ttcttataga atgcaatcgc caatttcttt ttccacacgg actacagata
     4501 catgtctcac gaccgcatcc tcattctcga cttcggcagc caggtgaccc agctcatcgc
     4561 ccgccgcgtg cgcgaagccc acgtctactg tgaagttcat cccaacgacg tgtctgcgga
     4621 attcatccgc gaatacaacc cgaagggcat cattctttcc ggctctcaca tgagcgccta
     4681 tgaagaaagc aacgaccagg ccagccaggc cgtgttcgaa gccggcgttc cggttctcgg
     4741 catctgctac ggcatgcaga cgatggccca gcagctcggc ggccgcgtcg aaagcggcac
     4801 gaagcgtgag ttcggctacg ccgaagtccg cgcccacggc cacaccaagc ttctcgaagg
     4861 cattgaggac ttccgcaccg aggaaggcca cggcatgctc aaggtctgga tgagccacgg
     4921 cgacaaggtt gccgagcttc ccccgggctt caaggtcatg tgctccacgc cctcctgccc
     4981 gatcgccggc atgtgcaacg aggaaaaggg cttttatgcc gtccagttcc atccggaagt
     5041 gacgcacacg ctcaagggcc gcgaaatgct caaccgcttc gtgctcgaca tctgcaagtg
     5101 ctccgccgac tgggtcatgg gcgactacgt cgccgaagcc gtcgcgcaga tccgcgagca
     5161 ggtcggtgac gaagaagtca ttctcggcct ctccggcggc gtcgactcct ccgtggccgc
     5221 cgcgctcatc caccgcgcca tcggcaagca gctcacctgc gtcttcgtcg acaacggcct
     5281 ccttcgcaag aacgaacgcg aacaggttcg tgaaacgttc gaaaagaaca tgggcctcaa
     5341 gctcatcgtc gtcgatgccg tcgaccgctt catgaacgaa ctcgccggtg tcaccgatcc
     5401 ggaacagaag cgcaagatca tcggccgcgt cttcgtcgag atcttccagg aagaagcctc
     5461 caagctcacg gccgccaagt ggctcgccca gggcacgatc taccccgacg tcatcgagtc
     5521 cgccggcgcc aagacgaaga aggccaagac catcaagtcc caccacaacg tgggcggtct
     5581 tcccgacacg ctgcacctca agctcctcga accgctgcgc tcccttttca aggacgaagt
     5641 ccgcgagctc ggcgtcgaac tcggccttcc ggccgaaatg gtctatcgtc acccgttccc
     5701 gggtccgggc ctcggcgtgc gcatcctcgg cgaagtcaag cgtgaatacg ccgaccttct
     5761 ccgtgaagcc gacgcgatct tcatcgaaga actccgcaac ttcgacctct acgacaaggt
     5821 gagccaggcc ttcgtggtct tcctcccggt caagtccgtg ggcgtcatgg gtgacggccg
     5881 cacctacgaa tgggtcgtga gcctgcgctg cgtcgaaacg tccgacttca tgaccgccgt
     5941 ctggagccac ctgccgtacg aacttctcgg caaggtttcc aaccgcatca tcaatgaagt
     6001 ccgcggcatc aaccgcgtcg tctatgacgt ctcgggcaag ccgcccgcca ccattgagtg
     6061 ggaataacct tctcgctctg acataaagtc aaaccctgaa aaactcttgt ttttcagggt
     6121 tttcttttgg ttcatctcgg aagtccgtgg aacgcggcct tgacttttaa gaaaaagtac
     6181 agatcgtctc ggaagccata agctcgtcgc ttaatttgct tgat
//
LOCUS       DBMDTI010000111         6223 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3207_length_6223_cov_2.398726, whole genome shotgun sequence.
ACCESSION   DBMDTI010000111 DBMDTI010000000
VERSION     DBMDTI010000111.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3207_length_6223_cov_2.398726"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            264..878
                     /gene="tmk"
                     /locus_tag="ACZPB6_06310"
     CDS             264..878
                     /gene="tmk"
                     /locus_tag="ACZPB6_06310"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR1184113.1"
                     /translation="MATGRFISFEGIDGAGKTTQIDGLERLIRAAGHEVVRTREPGGT
                     PLGEKIRGLLLTDEMTPRTETLLFFAARCEHAEKVIKPALERGAWVLSDRFTDATYAY
                     QTGGKGMKGEDVEALEAWTLAGFAPDVTVLFDLAPEVAARRRTIRAGEGDRFERESAD
                     FFARVRNAYLDRAARDPKRFVRVNAEEAPETIAAQLEKEFAKWL"
     gene            869..1912
                     /locus_tag="ACZPB6_06315"
     CDS             869..1912
                     /locus_tag="ACZPB6_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR1184114.1"
                     /translation="MALTQYPWLEEAAARLDAMRERLPNAILIHGMPGVGTFELARWF
                     AERLLCESPKADGSPCGKCPGCRMMHANGHPDCRIVVSEFLANALDLPYTPPETSSSS
                     KKLSREIRIHQFRALTDFLTMAPSRGGRRCVLVYPADMVRAEAAASLLKSMEEPPEGC
                     VFILAADDIDAVLPTIRSRSRLLRVGVPSKEDALAWLSAQKRLKGDPEVALAMAGGAP
                     LNAVKDITGLTLDAKASATLRDLLARGRSLTPDSIVRGYSASMGIPAVALHLSRWCYD
                     LLRVKSGADPHFFIREKDVLEKIAADATMKNLLSLNAAVAEMRRSAEHPLAPRQVWEG
                     VLLVYVRALGGQV"
     gene            complement(2339..2593)
                     /locus_tag="ACZPB6_06320"
     CDS             complement(2339..2593)
                     /locus_tag="ACZPB6_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184115.1"
                     /translation="MSQLFQFTPGDDSRISLARLQTFQRGRSYVAVEGRSAKEGNRVK
                     SCSFELPLKNHPSAETILSIGQNMKILQVLKIVNRRLFSV"
     gene            2668..3129
                     /locus_tag="ACZPB6_06325"
     CDS             2668..3129
                     /locus_tag="ACZPB6_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184116.1"
                     /translation="MLYPGTIFGIAAHVSGETAKAVSTAVLSMPASSFGKWSLVTDFG
                     QVKDLYRTLKRGPYAHLRDNPMMAFWMKWRGWFGALMLTVLGWGVYTLHVKHLVEKGH
                     LPICVESCPQRAIAFGPIDELRARYGANAASTGLPDPALTKPNLVVGLPKV"
     gene            complement(3216..4727)
                     /locus_tag="ACZPB6_06330"
     CDS             complement(3216..4727)
                     /locus_tag="ACZPB6_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1184117.1"
                     /translation="MSEKALKPALTDVGLRYNTKLADVLPPDLAEPLRTFGIETAEDL
                     YECAANAGASWFRPVTGIDASTATALMTWLHRNGRDVGEVTERFFLPGCAPSPESRSV
                     ATQASDEGIVPMERLVVPEGLRGDRGLNRAPAMACSLDAEDDLSAIRVWLQARASNPN
                     TQASYRKEAERYLLWCLLERRTALSSVRAGDAALFLRWLEGLGRTDEKAWAQQWRIPQ
                     SRWIGPKNMPRTSPAWRPFNGPLSATSRRNAVVVVRQLHNFLKNTGYLIFSPFDQVSP
                     KVPLLKGEGAPQAFADRSLTDEQWAEIVSRIDDLPEGWPRERMKLILMMGKSLGMRAS
                     EMLDARTGWIVERRVGFKVRAAIEIVGKGAKVRRLPLNDEQRTIIDDALRARGIAGVA
                     GSDPETPLLVNLGRGRNAHGPMTRSGLYVVLTKFLEEVALDVASERPMDAAKLRASST
                     HWLRHTFAVNALTEMSVNVVQAAMGHASVATTGRYLSPEEEAMSEAMEKMKAL"
     gene            4838..>6223
                     /locus_tag="ACZPB6_06335"
     CDS             4838..>6223
                     /locus_tag="ACZPB6_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HIR1184118.1"
                     /translation="MLSHIIRASLAGAAALGIASCAFGGAVSASYLGMGTSTGTPSGD
                     VLAIINPHQRDRSPIISRDGGRVPDDGLERPEAVGQLNFQRPMRIAVHNEPGSEPDAE
                     MINATKEFIRVMFGSQNVNIYYLNDRDLSQSIRTGGVDFFIADQSFYALEQAMGGVEQ
                     IAVMWPGTGDSPSEAMASTFFRKKPADGSAVHDPLTYVATHELAAMTPKSMSAWLVGA
                     GELVKRRRIGYEDLLSRTTFTNNSQRDLVEYVLAGRERVGLLPACALEQLALDPSFPM
                     DEIEVLNPKKKDGLSCVHSTEVYPGRVFAAVNDIDPTLKKAMTAVLLGMSSIKYGAEW
                     ALPVTNRPVFDLFYTLKIGPYSDLAGWSFQRFVREHAEIIALIMLVIFLVLTYTTTLS
                     ILVRRRTRALRDALGQRDRIEAEAAQSRQHIANLERTGIVGQMSTIIAHELKQPLGAI
                     TNTSNAARSISH"
BASE COUNT         1038 a         2001 c         2009 g         1175 t
ORIGIN      
        1 cttcggtgtt cttcttctct tcttgcgtca cgacgggaag tcctgggttg agtgttcggg
       61 accttcgggc ggcttgtgcc tccgaaggat gaagtgcggc acattttagc gcgggcggat
      121 gccggttcac ggcctgtgga gcccgccgag tcgtgagcgt ttgtttgaat gtgcgtcttc
      181 gtggcggcgc gggccgatcc gctacaatgc cgtagcgggt cgtccgagcg cggccccgag
      241 agtttcagtt cggagtttca cagatggcga caggacgctt tatttccttt gaaggcattg
      301 acggggcggg caagaccacg cagatcgacg ggctcgagcg cctcatcagg gccgcggggc
      361 atgaggtcgt gcgcacgcga gaacccggcg gcacgccgct cggggagaag atccgcggcc
      421 tgctccttac ggacgagatg acgccgcgca cggagacgct gctcttcttc gccgcccgct
      481 gcgagcatgc cgagaaggtg atcaagcctg cgcttgagcg cggcgcctgg gttctctccg
      541 accgcttcac cgacgccacc tacgcctatc agacgggcgg caagggcatg aagggcgagg
      601 acgtcgaggc gctcgaggcc tggacgctcg cgggctttgc gcccgacgtg acggtgctct
      661 ttgatctggc gcccgaggtg gctgcgcgcc gccgcacgat ccgtgcgggc gaaggcgaca
      721 ggttcgaacg cgagagcgcg gacttcttcg cccgcgtgcg caacgcctat ctggaccgcg
      781 ccgcccgtga tccgaagcgc ttcgtcaggg tcaatgccga ggaggcgccc gagacgatcg
      841 ccgcgcagct tgagaaggag ttcgccaaat ggctctgacg cagtacccct ggctcgagga
      901 ggccgccgca cgccttgacg cgatgcgcga aaggcttccc aacgcgatcc tcatccacgg
      961 catgcccggc gtgggcacgt tcgagctcgc ccgctggttc gccgagcgcc ttctgtgcga
     1021 aagcccgaag gccgacggct cgccatgcgg caagtgcccg ggctgccgca tgatgcacgc
     1081 caacggccat cccgactgcc gcatcgtcgt gagcgaattc ctcgcaaacg cgctcgacct
     1141 gccgtacacc ccgcccgaaa cctcctcgtc ctcgaagaag ctttcgcgcg aaatccgcat
     1201 tcatcagttc cgcgccctca cggacttcct cacgatggcg ccgagccgcg gaggacgccg
     1261 ctgcgtgctc gtctatcccg ccgacatggt gcgcgccgag gccgccgcct cgctactcaa
     1321 gtcgatggag gagccgcccg aggggtgcgt cttcattctt gcggccgacg acatcgacgc
     1381 tgtgctcccg acgatccgct cgaggtcgcg cctgctgcgc gtgggcgttc cgtcgaagga
     1441 ggacgcgctt gcatggctct cggcccagaa gcgcctcaag ggtgatcccg aggtcgcgct
     1501 cgccatggcg ggcggcgcgc cgctcaacgc cgtgaaggac atcacgggct tgacgctcga
     1561 cgcgaaggcc tccgccacgc tcagggacct gctcgcccgc ggccgcagcc tcacgcctga
     1621 cagcatcgtt cgcggctact cggcttcgat gggtattccc gcagttgcgc tccatctttc
     1681 ccgctggtgt tacgacctgc tgcgcgtgaa gtcgggcgcc gatccgcact tcttcatccg
     1741 cgagaaggac gtgctcgaga agatcgccgc cgatgcgacg atgaagaacc ttctctcgct
     1801 caatgccgcc gtcgccgaaa tgcgccgctc ggccgagcat ccgctcgcac cccgccaggt
     1861 ctgggagggc gtgctgctcg tctacgtcag ggcgctcggt ggccaggtct gagcgtatcg
     1921 tcatgaagtc ggcttcggtc aggtcggcgt agatgaatcg tctccttgcg gcggcgtgcc
     1981 tgatggtttg ctgtgtcggc ttctgtcgcc gcagattctt cgagctgggg gcgcgatcca
     2041 gagtactccg tttatctttc ggttgccgtc cagcccacgt tgccgtcgag ctttgtcatc
     2101 gaaacccttc agccgacgct cacttatttg cggaccacgc tgcccaatgc acgcattgga
     2161 atcaaatgca tgtctcccgc agaacttcat gaggcagtgc gccttcaaaa tgtccatgcc
     2221 ttcatcgccg aggccggcat ctttgccgac cttcaatctc agggactagc cgaacaactg
     2281 gctgcgtaca ggtctgccga tgcaaccgac cccgcatatg tcagtggcat ggccgtcgtt
     2341 agaccgaaaa aagtcttctg ttcacgatct tgaggacttg aagaatcttc atattctgtc
     2401 cgatgctcag gatagtttcg gcacttggat gatttttgag gggcagctcg aacgaacagg
     2461 acttgacgcg gttgccttcc ttagccgaac gaccttcaac agctacatag ctccgacccc
     2521 gctggaacgt ctggaggcgg gcgagggaga tgcggctgtc gtcgccaggt gtgaactgga
     2581 aaagctggct caacagggat tccctgcaga acactttcga gtagtcggcg atctccaaaa
     2641 tttgacgaac gctgttccag gaccgggatg ctttatccgg gcaccatctt cggcattgcg
     2701 gcacatgtga gtggggagac ggccaaggcg gtgagcaccg ctgtgctgtc gatgcccgcc
     2761 tcgtcgttcg ggaaatggtc gctggtcacg gattttggtc aggtcaagga tttgtacagg
     2821 acgctcaagc gcggaccata tgctcatctt cgggacaatc cgatgatggc cttctggatg
     2881 aagtggaggg gttggttcgg cgctttgatg ctgacggttc tgggttgggg tgtctatacg
     2941 cttcatgtga agcatctggt tgaaaaggga catctgccta tctgcgttga atcctgcccg
     3001 caaagggcga tcgcgttcgg tcccattgac gaacttcgcg ccagatacgg tgcgaacgcg
     3061 gcgtccaccg gacttcccga tcccgctctg acgaagccga acctcgtcgt cggattaccc
     3121 aaggtctaat tcttaaggcc ggaagaatcg gccccgcctt ccgtgaagga agatggggcc
     3181 gtgttgtctt ctgtgggcgt ctttccttcc cggaatcaaa gcgccttcat cttctccatc
     3241 gcctcgctca tcgcctcttc ttcgggcgag aggtatcggc ccgtcgtggc cacggaggcg
     3301 tgtcccatgg cggcctgcac gacgttgacg ctcatttcgg tcagggcgtt gacggcgaag
     3361 gtgtgcctga gccagtgggt ggacgaggcg cggagcttgg cggcgtccat cggacgctcg
     3421 gaggcgacgt cgagcgcgac ttcctcgagg aacttcgtga gcacgacgta tagtccgctt
     3481 cgggtcatgg ggccgtgtgc gttcctgccg cggccgaggt tgacgagaag cggcgtttcg
     3541 ggatcggacc ccgcgacgcc cgcaatgccg cgggccctga gcgcgtcgtc gatgatcgtg
     3601 cgctgctcgt cgttgagtgg caggcgcctt accttggcgc ccttgccgac gatctcgatg
     3661 gcggcgcgga ccttgaagcc cacgcgcctc tcgacgatcc agcccgtgcg cgcgtcgagc
     3721 atttctgagg cgcgcatgcc gaggctcttg cccatcatca gaatgagctt catgcgctcg
     3781 cgtggccatc cttcggggag atcgtcgatg cgggagacga tctcggccca ctgctcgtcg
     3841 gtgagcgagc ggtccgcaaa ggcttgcggg gctccttcgc ccttgaggag cgggaccttg
     3901 ggactcacct ggtcgaaggg gctgaagatg agatagccgg tgttcttgag aaagttgtgc
     3961 agctgacgca cgaccacgac ggcgtttctt cgactcgttg cggagagcgg gccgttgaag
     4021 gggcgccagg cgggcgaggt gcggggcatg ttcttgggac cgatccaacg gctctggggg
     4081 atgcgccact gctgcgccca tgccttctcg tcggtgcggc cgagtccctc gagccagcgc
     4141 aggaagagcg ccgcgtcgcc cgccctcacg gaggagagcg ccgtgcggcg ctcaagcagg
     4201 caccagagaa ggtagcgctc ggcctccttc ctgtagctcg cctgcgtgtt gggattggag
     4261 gcgcgtgcct gaagccagac gcggatggcc gagaggtcgt cctcggcgtc aagcgaacag
     4321 gccatggcgg gcgccctgtt gaggccccgg tctccgcgca ggccttcggg cacgacgagg
     4381 cgctccatgg gcacgatgcc ttcgtcggag gcctgggtcg cgacggagcg cgattcgggc
     4441 gagggcgcgc agcccggcag gaaaaagcgt tcggtcactt cgcccacgtc cctgccgttc
     4501 ctgtgaagcc aggtcatgag ggccgtggcc gtcgaggcgt cgatgcccgt gacggggcgg
     4561 aaccacgagg cgcccgcgtt ggcggcgcac tcgtagaggt cctcggccgt ctcaatgccg
     4621 aaggtcctga gcggctcggc gaggtctggc gggagcacgt ccgcgagctt ggtgttatat
     4681 cgtagcccga cgtcggtcag cgcaggcttc agtgcttttt cggacatgat tcggtcggca
     4741 ggtggcacaa ttaggacagt ttttagtacg aacattttgc ctcacccggc tgcagtgcgc
     4801 acggccacgc ccgcctgccg ccgacacaaa cccacctatg cttagccaca tcatcagggc
     4861 gtctcttgca ggcgccgcgg ccctcgggat cgcgtcgtgc gccttcgggg gcgccgtcag
     4921 cgcatcctac ctcggcatgg gcacgagcac gggcacgccc tcgggggacg tgctcgcgat
     4981 catcaatccg caccaacgcg accgctcgcc catcatcagc cgcgacggcg gcagagtgcc
     5041 cgacgacggc ctcgagcgtc ccgaggccgt gggacagctg aacttccagc gtccgatgcg
     5101 catcgcggtc cacaacgagc ctggatcaga gcctgacgcc gagatgatca acgcgacgaa
     5161 ggagttcatc cgcgtcatgt tcggctcgca gaacgtgaac atctattatc tgaacgaccg
     5221 cgacctgtcg cagtcgatcc gcacgggagg cgtcgacttc ttcatcgcgg accagtcctt
     5281 ctacgccctc gagcaggcca tgggcggcgt cgagcagatc gccgtcatgt ggcccggcac
     5341 gggcgacagt ccgagcgagg cgatggcctc gaccttcttc cgcaaaaagc ccgccgacgg
     5401 ctccgcagtg cacgatccgc tcacctacgt cgccacgcac gagctcgccg ccatgacccc
     5461 gaagtcgatg tcggcctggc tcgtcggcgc gggcgagctc gtcaagcgcc gccgcatcgg
     5521 ttacgaggac ctgctctcga ggacgacctt cacgaacaac tcgcagcgcg acctcgtcga
     5581 atacgtgctt gcgggcaggg agcgcgtggg gcttctgccc gcctgcgcgc tcgagcagct
     5641 cgcgcttgat ccgtcctttc cgatggacga gatcgaggtt ctcaatccca agaagaagga
     5701 cggtctctcc tgcgtccact cgaccgaggt ctatcccggg cgtgtttttg cggccgtcaa
     5761 cgacatcgat ccgacgctca agaaggcgat gaccgccgtg ctgctcggca tgtcgagcat
     5821 caagtacggg gccgaatggg cgctgcccgt gacgaaccgt cccgtcttcg atctcttcta
     5881 cacgctcaag atcggtccct acagcgacct tgcgggctgg agcttccagc gcttcgtgag
     5941 ggagcacgcc gagatcatcg cgctcatcat gctcgtgatc ttcctcgtgc tcacgtacac
     6001 gacgacgctc tcgatcctgg tgcgccgccg cacgagggcg cttcgcgacg ccctgggcca
     6061 gcgagaccgc atcgaggccg aggccgcgca gtcgaggcag cacatcgcga acctcgagcg
     6121 cacgggcatc gtgggccaga tgtcgaccat catcgcgcac gagctcaagc agcctctggg
     6181 cgccatcacc aacaccagca acgccgcacg atccatcagc cac
//
LOCUS       DBMDTI010000112         6192 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3222_length_6192_cov_2.777869, whole genome shotgun sequence.
ACCESSION   DBMDTI010000112 DBMDTI010000000
VERSION     DBMDTI010000112.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3222_length_6192_cov_2.777869"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="ACZPB6_06340"
     CDS             complement(<1..213)
                     /locus_tag="ACZPB6_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR1184119.1"
                     /translation="MTDGEVIYPEQIEVPAVAVRDMVIFPSTLVPVFMAREASVNAAR
                     KAFQSDNKYVFAVTQKEPDTDAPKQDD"
     gene            complement(310..1539)
                     /gene="clpX"
                     /locus_tag="ACZPB6_06345"
     CDS             complement(310..1539)
                     /gene="clpX"
                     /locus_tag="ACZPB6_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR1184120.1"
                     /translation="MRTCSFCGRSESECKELFSGIRGELICGECIRSMNDELRSGPTP
                     ESAPEAKPEAEKPLPKPKELYDLLDQYVVGQERAKRTLSVAVYNHYKRLKNQEDAARS
                     VELQKSNILLIGPTGSGKTLLAQTLARALDVPFAIADATTLTEAGYVGEDVENIVAKL
                     VQAADGDIERAQRGIIYLDEIDKIARKSENPSITRDVSGEGVQQALLKLVEGTVASMP
                     AQGGRKNPGKAMIQVDTSQILFICGGAFDGMQSIISRRTERAEMGFGGRVFSKDGDKS
                     LTKVYSQVEPADLVKYGLIPELVGRLPVIAALQELDEEALIAILTKPKNAIVRQYQAL
                     LGMEDVELEFTPDALKAVAEKAIARKTGARGLRSIVEGVLLDAMFDIPTRGNIARARV
                     TRETVEKSAPVELIERD"
     gene            complement(1560..2159)
                     /gene="clpP"
                     /locus_tag="ACZPB6_06350"
     CDS             complement(1560..2159)
                     /gene="clpP"
                     /locus_tag="ACZPB6_06350"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR1184121.1"
                     /translation="MLVPYVIEDTGRGERSYDIYSRLLRDRIIFLTGEVNDQSASLVI
                     AQMLFLESENPDKDIYFYINSPGGSVSAGMGIYDTMKFIRPKVCTICVGLAASMGALL
                     LSSGEKGRRYALPHSRVMIHQPLGGIQGQATEIEITARQILRTKQELNQILADNCGKP
                     VSEVSLDTERDYWLVGEEAMNYGLVDRIFTTRAELEKAL"
     gene            complement(2343..3671)
                     /gene="tig"
                     /locus_tag="ACZPB6_06355"
     CDS             complement(2343..3671)
                     /gene="tig"
                     /locus_tag="ACZPB6_06355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1184122.1"
                     /translation="MTEQETVKTEEVAKVNPLERTLDLVVSYASAEELTEKALKNYGK
                     NAKLPGFRKGHVPAAQVRRMYGMQAYDEAINQLVGEAWQKAASESELRIAGYPRIDAA
                     KNEEDKENMHFTAKFEVFPDVQVPDFSAVELKRYVCPVTDAEVEKTIDVMRRQRATYN
                     VVERAAANDDRVKLNFKGKKDGVEFQGGSAEGYVFVLGQGRMLPEFEAAVTGMAAGEK
                     KTFPMTFPADYGIKELEGQAVEFDVEVVEVAEPAYPEVDDEFAASLGVKGGVEAMRAE
                     IRANLEREVKARLETKTKAEVMEAAANACDFALPTVLVNDQCEALRQQMESDLAGRGI
                     DVKKMPAMPVDAFKPQAERRVRLGLFVEALISQENISGSDEQVREIAADIASSYEKPE
                     EVIEYIMKDQNRVGNLRAQATENNVTEWILAKAKTVEETVEFDKLMAGQF"
     gene            4107..4673
                     /locus_tag="ACZPB6_06360"
     CDS             4107..4673
                     /locus_tag="ACZPB6_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184123.1"
                     /translation="MRSRLSLLTISFAMLSLLAGCATAPRGTNTDAGTQQEVTSTRRH
                     TTAERFAKTTDNELYNERIAQVSRDIRAICTSPANRPYYAKTPCLPSGMTEAHLKDGS
                     RITPEARRVAERVFASLHKLNEDTRSLMISSGDAHLIKLARHSREMVDPKISALQSSL
                     LKGAMTWAEYNRARLEVYDASRTGAPAE"
     gene            4737..4822
                     /locus_tag="ACZPB6_06365"
     tRNA            4737..4822
                     /locus_tag="ACZPB6_06365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4771..4773,aa:Leu,seq:tag)
     gene            complement(4925..5707)
                     /locus_tag="ACZPB6_06370"
     CDS             complement(4925..5707)
                     /locus_tag="ACZPB6_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HIR1184124.1"
                     /translation="MTTFTKLLPPEAAPAQPILARAKQLVLTSAQRMAWEDSFETPEG
                     VIAVSVEEKRPLFVNDVFVDDAGQFWVVRPAVEKVLHVTGDLRTMQEAVGALINRGVE
                     VAEAPEGFAVRPLPNIAKMLGMIGLEVTEVEEAFEPVRIERHAGGCGCGGGCGCGGHH
                     HHHGEEECGCGCGGHHHDEEECGCGCGGHHHHHAEEECGCGCGGHDHHHEEACGCGCG
                     EHHHHHEDECGCGEHHHHHDGKEHHHHHHEEGACGCGCGHKH"
     gene            complement(5834..>6192)
                     /locus_tag="ACZPB6_06375"
     CDS             complement(5834..>6192)
                     /locus_tag="ACZPB6_06375"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR1184125.1"
                     /translation="LAVLHRHAGVGCFDQDVFVNAVGGVKISEPAADLALLCAIYSSI
                     RNKPLRRGLVVFGEVGLAGEIRPAPRGQERLREAAKLGFAKAVIPRANAPRTPVEGLE
                     VIAVDRLSDALAAAVE"
BASE COUNT         1096 a         1888 c         1922 g         1286 t
ORIGIN      
        1 gtcgtcctgc ttgggcgcat cggtgtcggg ctccttctgc gtgaccgcga aaacgtactt
       61 attgtcggat tgaaaagcct tcctagcggc gttgacggaa gcctcgcgcg ccatgaagac
      121 gggcaccagc gtcgagggga agatcaccat gtcccgcact gcgacagcag gcacttcaat
      181 ttgttcgggg taaatcactt ccccgtcggt catgttgtcc aaatctctag tcctagtgaa
      241 tgttcggttc ggtcttgaat atatgggggc cagagccccc gttttcaagt cgtatgcaga
      301 tcagtttcgt cagtcgcgct cgatgagctc aacgggtgcg ctcttctcga ccgtctcacg
      361 cgtaacgcgt gcccgcgcga tgtttccacg cgtcggaatg tcgaacatgg catcaagaag
      421 cacgccctcg acgatggagc gcaggcctcg ggcgcccgtc ttgcgggcga tggccttttc
      481 ggcgaccgcc ttgagcgcgt cgggcgtgaa ctcgagttcg acgtcctcca tgccgagcag
      541 ggcctgatac tggcggacga tggcgttctt gggcttcgtg aggatggcga tgagcgcctc
      601 ctcgtcgagc tcctgaagcg cagcgatcac gggcagacgg ccgacgagct cggggatcag
      661 gccgtacttc acgaggtcgg cgggctcgac ctggctgtac accttggtga ggctcttgtc
      721 gccgtccttg ctgaagacgc ggcctccaaa gcccatctcc gccctttcgg tgcggcggct
      781 gatgatgctc tgcatgccgt cgaaggcgcc gccgcagatg aagaggatct gcgaggtgtc
      841 gacctgaatc atggccttgc cgggattctt gcggccgccc tgggcgggca tgcttgccac
      901 ggtgccctcg acgagcttga gcagcgcctg ctgcacgcct tcgccggaca cgtcgcgcgt
      961 gatcgacggg ttctcgctct tgcgagcgat cttgtcgatt tcgtcaaggt agatgatgcc
     1021 tcgctgggcg cgctcgatgt cgccgtcggc ggcctgcacg agcttggcga cgatgttctc
     1081 gacgtcctcg cccacgtagc ccgcctcggt gagcgtcgtc gcgtcggcaa tggcgaacgg
     1141 cacgtcgagc gcacgggcaa gcgtctgcgc cagcagcgtc ttgccggagc ccgtcggacc
     1201 gatcagaaga atgtttgact tctgaagctc gacggagcgc gcagcgtcct cctggttctt
     1261 cagtcgcttg tagtggttgt agaccgccac ggatagcgtg cgcttggcgc gctcctgacc
     1321 tacgacgtac tggtcgagca ggtcatagag ctccttgggc ttgggcaggg gcttctcagc
     1381 ctcgggcttc gcctcgggag cgctctcagg agtggggccg ctcctgagct cgtcgttcat
     1441 cgagcggatg cattcaccgc agatcagttc gccgcgaatg ccggagaaga gctccttgca
     1501 ctcgctctcg ctgcggccgc agaaggaaca ggttcgcatg tcattcacct cgattccgct
     1561 caaagcgcct tttcaagctc ggcgcgcgtc gtgaagatgc ggtcgacgag gccgtagttc
     1621 atggcctcct cgcccacgag ccagtagtca cgctcggtgt cgagggagac ttcggaaaca
     1681 ggcttgccgc agttgtcggc aagaatctgg ttgagctcct gcttcgtgcg caggatctgg
     1741 cgggcggtaa tctcgatttc ggtcgcctgc ccctggatgc cgccgagcgg ctgatggatc
     1801 atgacgcggg agtgcggcag cgcatagcgg cggcccttct cgcccgagct caggaggagc
     1861 gcgcccatcg aggcggcaag gcccacacag atcgtgcaga ccttcggacg aatgaacttc
     1921 atcgtgtcgt agatgcccat gccggcggaa acggaaccgc cggggctgtt gatgtagaag
     1981 tagatgtcct tgtccggatt ctcgctctcg aggaagagca tctgtgcaat cacgaggctc
     2041 gcggactggt cgttcacctc acccgtaagg aagatgatgc ggtcgcgaag gaggcgcgaa
     2101 tagatgtcgt agctgcgttc gccgcggccg gtgtcttcaa tgacataagg gactagcatt
     2161 tttttattcg ccttgtggaa aaaggggtat ctgccgattg tagcggagcg agcggaaaag
     2221 ctcccgcctg cggaccacgg aaaagaaaaa agaggcatcc ggcgtgttgt ccgtgatgcc
     2281 tctcgagtcg atcagggccg gcgcggcagg ccgcgcccgc aagtctgacg ttgtcccgtt
     2341 cgttagaact ggccggccat cagcttgtcg aattcaaccg tttcctcaac ggtcttggcc
     2401 ttggcgagga tccattcggt cacgttgttt tccgtggcct gggcgcggag gttgcccacg
     2461 cggttctggt ccttcatgat gtactcgatg acttcttccg gcttttcgta gctggaagcg
     2521 atatcggcgg cgatttcacg aacctgttcg tcggaaccgg agatattttc ctgagagatc
     2581 agggcttcga cgaagaggcc gaggcgcacg cggcgttcag cctgcggctt gaaggcgtcg
     2641 acgggcatgg cgggcatctt cttgacgtcg atgccgcggc cggcgaggtc gctttccatc
     2701 tgctggcgga gggcttcgca ctggtcgttc acgagaacgg tcggaagggc gaagtcgcag
     2761 gcgttggcgg cggcttccat cacttcggcc ttggtcttcg tttcgaggcg ggccttgact
     2821 tcgcgttcga ggttggcgcg gatttcagcg cgcatggctt caacaccacc cttgacgccg
     2881 aggctggcgg cgaattcgtc gtcaacttcc gggtaggcgg gttcggcgac ttcgacgact
     2941 tcaacgtcga attcaacggc ctgaccttcg agttccttga tgccgtagtc ggcggggaag
     3001 gtcatcggga aggtcttctt ttcaccggca gccatgcccg tgacggcggc ttcgaactcg
     3061 gggagcatgc ggccctggcc gagaacgaac acgtagcctt cggcggaacc gccctggaat
     3121 tcaacgccgt ccttcttgcc cttgaagttg agcttgacgc ggtcgtcgtt ggcggcggcg
     3181 cgttccacga cgttgtacgt ggcgcgctgg cggcgcatga cgtcaatcgt cttttcgact
     3241 tcggcgtccg tcaccgggca gacgtagcgc ttgagttcga cggcggagaa gtcgggaacc
     3301 tgaacgtcag ggaacacttc gaacttggcc gtgaagtgca tgttctcctt gtcctcttcg
     3361 ttcttggcgg cgtcgatgcg cggatagccg gcaatgcgga gctcgctttc ggaggcggcc
     3421 ttctgccagg cttcgccgac gagctggttg atggcttcgt cataggcctg catgccgtac
     3481 atgcggcgga cctgagcggc cggaacgtga cccttgcgga agcccgggag cttggcgttc
     3541 ttgccgtagt tcttcagggc cttttcggtg agttcttcag cggaagcgta ggaaacgacc
     3601 agatcgagcg tgcgttcgag cgggttgacc ttggcgacct cttcggtctt gacggtttct
     3661 tgttcagtca tcgcagtagt ctttataaaa gtccgcccgc ttgacgcgcg gcggaatttg
     3721 tggaagagat ccgtcccttt gcagaacgga ccgcgtgaaa aatcctttcc agagcgaagc
     3781 gcacccgcat gcggcggacc gtcgttcgtt ctcttctata aaaagagcat ccgccggctt
     3841 ctgatgtcgg cggacctgaa ttttttaatt atagcggaga aattcagctg ttgtttcaac
     3901 gggttcctgc gggaaacccg ctccccgcac tgaattcggc ctcgattcag gcgccgtcct
     3961 cctcttgtgc aggagacgcc ttcctccgca tccgcacggc caattgctta caattcctgc
     4021 gacgaggctc tttcgagctt gctagaattt ttctcccgtt caatccggcc tgcgccggcc
     4081 ctcatcttcc tcctgccttc agaaccatgc ggtcccgtct ttcccttctg accatctcct
     4141 tcgccatgct ctcccttctt gcaggctgcg caactgcgcc cagggggacg aacactgatg
     4201 cggggacgca acaggaagtc acgagtaccc gccgtcacac gaccgcagaa cgctttgcaa
     4261 agaccaccga caacgaactc tacaacgaga ggatcgctca ggtgtcaagg gacatacgcg
     4321 ccatctgcac ctcgccagca aaccgtcctt actatgcgaa gacgccctgc ctgccctcgg
     4381 gcatgacgga agctcacctc aaggacggct cgcgcatcac cccggaggcg cgacgcgtcg
     4441 ctgagcgcgt ctttgcaagc ctgcacaagc tcaacgagga cacccgcagc ctgatgatct
     4501 cctcgggaga cgcccacctc atcaagcttg cgcgccattc ccgtgaaatg gtcgatccaa
     4561 aaatctccgc cctgcagtcc tcgcttctca agggcgccat gacctgggcc gagtacaacc
     4621 gcgcccgtct cgaggtctac gatgcaagca ggacgggcgc tcccgccgaa tgacacgcca
     4681 tcaggctcaa ggtcaggaca tccgcgccct gaattcctgt agaatgcgtt gttcgtgcgg
     4741 ggatggcgaa attggtagac gcaccaggtt taggtcctga cgccgttgag gcgtgtcggt
     4801 tcgagtccga ttccccgcac caaggattaa gaaagaaaag ggagacgctt ccgagtgtct
     4861 cccttttctt cattcagccc gccgcagggc aaatggggcg cggccgtgcg caccccttca
     4921 caagtcagtg cttgtgaccg caaccgcagc cgcaggcgcc ttcctcgtga tgatggtgat
     4981 gatgctcttt accgtcatga tgatggtggt gctcgccgca accgcactca tcttcatggt
     5041 ggtgatgatg ttcgccacag ccgcaaccac acgcttcctc gtggtgatgg tcatgaccgc
     5101 cgcaaccgca gccgcattcc tcttcggcat gatggtggtg atggccgcca cagccgcagc
     5161 cgcactcttc ctcgtcgtgg tggtgcccgc cgcagccaca gccgcattcc tcttcgccgt
     5221 gatggtgatg atgaccgccg cagccgcaac cgccgccaca gccgcagccg cctgcatgac
     5281 gctcgatgcg gacgggttcg aaggcctctt cgacctcggt gacctcgaga ccgatcatgc
     5341 cgagcatctt ggcgatgttc gggagcggcc gaacggcgaa gccctcggga gcttcggcca
     5401 cttcgacgcc gcggttgatg agggcgccga cggcctcctg catcgtgcgc aggtcgcccg
     5461 tcacgtgaag aaccttttca acggcgggac gcacgaccca gaactggcct gcgtcgtcaa
     5521 cgaaaacgtc gttgacgaag agcgggcgct tttcctcaac ggagacggcg atcacgcctt
     5581 cgggcgtttc aaaggagtcc tcccaggcca tgcgctgggc ggacgtgaga acgagctgct
     5641 tggcgcgggc gagaatgggc tgggcagggg ccgcttcggg cggcaggagc ttggtaaaag
     5701 tcgtcatttt gaattcctgt gaggccggcg cctcgcgaag cgacaggctt cgccctcagg
     5761 cgccgacaag tgtctaccca ttttaagcaa agagaatcat tcgcgtgtaa gaaaactttg
     5821 cgcccggcaa aggctattcg acagcggccg ccagcgcatc ggaaagacgg tcgacggcaa
     5881 tgacctcgag gccctcgacg ggcgttctcg gcgcattggc gcgcggaatc acggcctttg
     5941 cgaagccaag cttcgccgcc tcacgaagcc tttcctgacc tctgggcgca ggcctgattt
     6001 ctccggcgag gcccacttcg ccgaagacaa cgagaccgcg gcgcaggggc ttgtttcgaa
     6061 tgctcgaata gatggcgcac aaaagcgcga gatcggccgc tggctccgag atcttcacgc
     6121 cgcccacggc gttgacgaag acgtcctgat caaagcagcc cacgcccgcg tggcggtgca
     6181 ggaccgcaag aa
//
LOCUS       DBMDTI010000113         6181 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3227_length_6181_cov_2.179441, whole genome shotgun sequence.
ACCESSION   DBMDTI010000113 DBMDTI010000000
VERSION     DBMDTI010000113.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3227_length_6181_cov_2.179441"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="ACZPB6_06380"
     CDS             complement(<1..191)
                     /locus_tag="ACZPB6_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012512.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR1184126.1"
                     /translation="MMKRHDIRVVHAPEDRILEARALLARRGIYCEHTTAANYAAWGR
                     YCELYGPTPDTLITMCGAG"
     gene            complement(188..490)
                     /locus_tag="ACZPB6_06385"
     CDS             complement(188..490)
                     /locus_tag="ACZPB6_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012512.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR1184127.1"
                     /translation="MKAYVYETFEQLGRIPANIVIPVGNGTLFIGAVKALEHLHASGA
                     IERFPNIIALQSEKCDPLYRAREAGLEDPARVTPAPTMAERASPSAIRCAAARSLT"
     gene            complement(576..959)
                     /locus_tag="ACZPB6_06390"
     CDS             complement(576..959)
                     /locus_tag="ACZPB6_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR1184128.1"
                     /translation="MKFVQPPFKGENKGHYTPGIISKGMLYVSGQLSIDPDTREVCRG
                     DIRAHARLALGNVDRVLKEAGVRRDQVVYCRVFTTKGEYWGPINEVYAEFFGAHKPGR
                     LVATVPELHFGCLVEIEAVAELDEN"
     gene            complement(1041..1700)
                     /locus_tag="ACZPB6_06395"
     CDS             complement(1041..1700)
                     /locus_tag="ACZPB6_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1184129.1"
                     /translation="MKPILAPYVPIADLVAKTFGEDVEVVLHDLSDPQHSVVYVANNK
                     VTGRQVGESFRHLITKAIHSPGESGDVNPNFYFRHEGRLIRSSSSIIRDENGHIAGAF
                     CINVDTTRITRQIDMLAQMLPGIDKGVLPLLPDEENEDVCPVDTNRTVMEIMTDLVDN
                     ITGDIPSGSMTREKRIELIRFMDQRGVFLVKGAIDRVAEKLGISKVTVYSYLDEVRGK
                     R"
     gene            1966..3969
                     /locus_tag="ACZPB6_06400"
     CDS             1966..3969
                     /locus_tag="ACZPB6_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184130.1"
                     /translation="MDELIYGVWKGRAYDNRRTIDGKAPEGLPLSEFLAFNSGNPVAG
                     FAGPQGFLMFDNRVTLADVLLSYYKRVRELACGRCTPCRAGGVMIVEALEAACEGRGS
                     EVDWERIRDIALQMKETSLCGIGLTTPEPILGALEHFPELLVNAPVMPATQRDFISVA
                     TAPCIEACPAKVNVPRYIDYIRDGHPELATGVLLRHYPLVGSCGRVCVRPCETACARN
                     HVDRPVAIKDLKRYAADYCGQTIEALFRNERVEPTPVSKRVAVIGAGPAGINCAYHLL
                     RRGHMVDIYEEHAGAGGMARLGIPAYRLPNSLLETETTAIERMGGQYFFNSRFGRDFT
                     LDSLFRRGYNAVFLGVGCALGQYLNLPGEDKSADGYLKGLDFLVDVEGHQSSGEPIEL
                     DGDVVVVGCGNVAMDCCRTARRLMKGGRVTVSYRRTRASAPADPEEIKAAMEEDIGFE
                     FLTAPVEVIVEDGRVTGIRLVRMAEGEPDASGRRSVRPVEGSEFVVPCRYVIAAIGQK
                     LDTSIFCEADGIRLTKRGTIEVDESLRTSREGVFAGGDASSGPTSLIWGMAQGQNAAM
                     AIHEYLMTDAPGFNPRLRFADLIRKADLLGKCVPDRPVVFRPRREIGMLSAAERVGNW
                     EEVEHGLTESDAREEAKRCLRCYRLFGVKTLFPIPGKSAMDEL"
     gene            3992..5809
                     /locus_tag="ACZPB6_06405"
     CDS             3992..5809
                     /locus_tag="ACZPB6_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HIR1184131.1"
                     /translation="MLATINLQPVEFTEGETILEVARRTGTFIPTLCEFAALNHRPGT
                     CRMCLVEVTRADGTSRVVTACETRLVEGDVVDTYSHKVRRMQKLQAELLFSDHCETCS
                     GCARHGACELQKVARVVGLDVTRLSGRLATRPVETDASANGLVFTPDKCIRCLRCIEA
                     CRQVQGIGVIKLDHTGTNAAVSFGGPWGESETCIQCGQCALVCPTGALAVKDQTDCAL
                     DWFDDPAVTTVVQFAPAVRVTIGESIGARPGENLQGRIVAALRKLGADCVMDTRWSAD
                     VTIMEEGTELLERLLRQKEEGTLHGHPDTMFTSCCPGWINHIEKNCPDMIPHISSTRS
                     PQAIFGALAKTWLPKTLGIPAERIRSISIMPCTAKKDEAARELLKHGGEQDVDLVLTV
                     QEFAAMLDRRGIDLMSLVPAEFDSPFMSEGSGAAQLFATTGGVMEAALRTVSALAGGP
                     DLGRIAFEPVRGLATFKEAEVETEAFGKLRIAVVHGMRAADEVIRLVREGRSPYHFVE
                     VMACPGGCVGGGGTVRGIVWRSTLDRRQNAVYSTDASMKLRTSHENEDVVRLYRDFLG
                     EPGSPLAHELLHCEYRERERRSEKPDYRTIESAVELASV"
     gene            5932..>6181
                     /locus_tag="ACZPB6_06410"
     CDS             5932..>6181
                     /locus_tag="ACZPB6_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate/malate family dehydrogenase"
                     /protein_id="HIR1184132.1"
                     /translation="MKAPVRVTVTGPAGQIGYATLFRIASGAMFGPDQPVFLTLLERA
                     NPTSAAALKGVMMELSDCAFPLLAGMRAVQDPVEAIRDC"
BASE COUNT         1052 a         2023 c         2035 g         1071 t
ORIGIN      
        1 atgcctgcgc cgcacatggt gatgagcgtg tcgggcgtgg ggccgtagag ctcgcagtag
       61 cggccccagg cggcgtagtt ggcggccgtg gtgtgctcgc agtagatgcc gcggcgggcg
      121 agcagggctc gggcctcgag gatgcggtcc tcaggagcgt gcacgaccct gatgtcgtgg
      181 cgcttcatca tgtcaaggat ctcgcggccg cgcatcggat cgccgatggc gatgccctct
      241 cggccatcgt gggcgcgggc gtgacgcgtg caggatcctc aagccccgcc tcgcgggcgc
      301 ggtagagcgg atcgcacttc tcgctctgga gcgcgatgat gttggggaag cgctcgattg
      361 cccccgaggc gtgcagatgc tcgagcgcct tgacggcgcc gatgaagagc gtgccgttgc
      421 cgacgggaat cacgatgttg gccgggatac ggccgagctg ctcgaaggtc tcgtagacgt
      481 aggccttcat gggtgtcggc ggcgcatgcg gcgccgcagg aggtgcagac gtagccggag
      541 gtcatgtggc tgtctcccag aaaaagagat gcggatcagt tctcgtcgag ctcggccacg
      601 gcctcgatct cgacgaggca gccgaagtgc agctcgggca cagtggcgac gaggcggccg
      661 ggcttgtgcg cgccgaagaa ttcggcgtag acctcgttga tcgggcccca gtactcgccc
      721 ttcgtggtga agacacggca gtagacgacc tggtcgcggc gcacgccggc ctccttgagc
      781 acgcggtcga cgttgccgag cgcaaggcgc gcatgggcgc ggatgtcgcc ccggcagacc
      841 tcgcgcgtgt cgggatcgat cgagagctgg cccgagacgt agagcatgcc cttcgagatg
      901 atgcccggcg tgtagtggcc cttgttttca cccttgaacg ggggttggac gaatttcatc
      961 agagtctcct tgtctggtct gtgaatgcag aacgccccgg accgcaggtc acgcggttcg
     1021 gggcgagaaa caggtggcgg tcagcgcttg ccgcggactt cgtcgaggta ggagtagacg
     1081 gtcaccttcg agatgccgag cttttcggcg acgcggtcga tggcgccctt cacgaggaag
     1141 acgccgcgct ggtccatgaa gcggatgagc tcgatcctct tttctcgcgt catcgagccc
     1201 gagggaatgt cgcccgtgat gttgtcgacg aggtccgtca tgatctccat cacggtgcgg
     1261 ttcgtgtcga cgggacagac gtcctcgttt tcctcgtcgg gcaggagcgg aagtacgccc
     1321 ttgtcgatcc cggggagcat ctgagcgagc atgtcgatct ggcgggtgat gcgcgtcgtg
     1381 tcgacgttga tgcagaacgc acccgcaatg tgcccgttct cgtcacggat gatcgaggag
     1441 gagctgcgga tgaggcgccc ctcgtggcgg aagtagaagt tcgggttgac gtcgcccgat
     1501 tcgcccggcg aatggatcgc cttcgtgatc agatgacgga agctttcgcc cacctggcgg
     1561 cccgtcacct tgttgttggc gacgtagacg accgaatgct gcggatccga caggtcgtga
     1621 agaacgactt caacgtcttc gccgaaggtc ttggcaacaa ggtcggcaat cggaacgtac
     1681 ggggcgagta tgggcttcat aagtggatat ataaaaaaat ttattgcttg taaaaatttt
     1741 tctagctata atttcctccc gacgggaacc cttgcctgaa cgccggctga aggccgcgtc
     1801 gggacggtgc tgagaaccgc tagaccccat catacagtgg cggctcctct gcgtctataa
     1861 aaaactatac acttgacggg ttcccgtaga cttttatgct gaaactcccg gtccgcgcat
     1921 gaaaaggcgc gcccggccga atcctgcttg agaggatgtt ttcatatgga cgagctgatt
     1981 tacggcgtct ggaagggacg cgcgtacgac aaccgccgca cgatcgacgg aaaggcgccc
     2041 gagggccttc cgctgagcga attcctcgcc ttcaacagcg gcaatcccgt tgcgggcttt
     2101 gccggtcccc agggcttcct gatgttcgac aatcgcgtga cgctcgcgga cgtgctgctt
     2161 tcctattaca agcgcgtcag ggagctcgcc tgcggccgct gcacgccgtg ccgtgcgggc
     2221 ggcgtgatga tcgtcgaggc gcttgaagcc gcctgcgagg gcaggggatc cgaagtcgac
     2281 tgggagcgca tccgcgacat tgcccttcag atgaaggaga cgagcctctg cggcataggc
     2341 ctcacgacgc ccgagcccat tctcggcgcg ctcgagcact ttcccgagct tctcgtcaac
     2401 gcgcccgtca tgcccgccac ccagcgcgac ttcatcagcg tggcgacggc cccctgcatt
     2461 gaggcctgtc ccgcgaaggt gaacgtgccg cgctacatcg actacatccg cgacggccac
     2521 cccgagctcg ccacgggcgt gttgctgcgc cattatccgc tcgtcggctc ctgcggccgc
     2581 gtgtgcgtgc gcccctgcga gacggcctgc gcgcgcaacc acgtcgaccg ccccgtcgcg
     2641 atcaaggacc tcaagcgcta tgcggccgac tactgcggcc agacgatcga ggcgctcttc
     2701 aggaacgagc gcgtggagcc cacgcccgtc tcgaagcgcg tcgccgtgat cggcgcgggt
     2761 cccgccggca tcaactgcgc ctaccatctg cttcgccgcg gccacatggt cgacatttat
     2821 gaagagcatg cgggcgcggg cggcatggct cgcctcggca ttcccgccta ccgtctgccg
     2881 aactcgcttc tcgagacgga gacgaccgcg atcgagcgca tgggcggcca gtacttcttc
     2941 aacagccgct tcgggcgcga cttcacgctc gactcgctct tcagacgcgg ctacaacgcg
     3001 gtcttcctcg gcgtgggctg cgcgctcggc cagtacctca acctgccggg cgaggacaag
     3061 tccgccgacg gctacctcaa ggggctcgac ttcctcgtcg acgtcgaggg ccaccagagc
     3121 agcggcgagc cgatcgagct tgacggcgac gtcgtggtcg ttggctgcgg caacgtcgcc
     3181 atggactgct gccgcacggc ccgccgcctg atgaagggcg gcagggtgac ggtctcctac
     3241 aggcgcaccc gcgcctccgc ccccgcggac cccgaggaga tcaaggccgc catggaggag
     3301 gacatcggat tcgaattcct gacggcgccc gttgaggtga tcgtcgagga cggccgcgtc
     3361 acgggcatcc gcctcgtgcg catggccgaa ggcgagcccg acgcctcggg ccgccgttcc
     3421 gtcaggcccg tcgaggggag cgaattcgtc gtgccgtgcc gctatgtgat tgccgccatc
     3481 ggccagaagc tcgacacgtc gatcttctgc gaggccgacg gcatccgact cacgaagcgc
     3541 ggcaccatcg aggtcgacga gtcgctgcgc acttcgcgcg aaggcgtctt cgcgggcggc
     3601 gacgcctcct cgggcccgac gtccctcatc tggggcatgg cccagggcca gaacgccgcc
     3661 atggcgatcc acgagtacct gatgaccgac gcaccggggt tcaatccgcg cctgcgcttt
     3721 gcggacctca tcaggaaggc cgaccttctc ggcaagtgcg tgcccgaccg tcccgtcgtc
     3781 ttcaggcccc gcagggagat cggcatgctg tccgccgccg agcgcgtggg caactgggag
     3841 gaagtcgagc acggactcac cgagtccgac gcccgtgagg aggcgaagcg ctgcctgcgc
     3901 tgctaccgcc tcttcggcgt gaagacgctc ttcccgatcc ccggcaagag cgccatggac
     3961 gaactctgat cgcgtgaagg agacccccag aatgctggcc acgatcaatc tacagcccgt
     4021 cgagttcacc gagggcgaaa ccattctaga agtcgcccgg cgcacgggca ccttcattcc
     4081 gacgctttgc gaatttgcgg cgctcaatca ccgtccgggc acttgccgca tgtgcctcgt
     4141 cgaggtcacc cgcgccgacg gcacgagccg cgtggtgacg gcctgcgaga cccgtcttgt
     4201 cgagggcgac gtcgtcgaca cctactcgca caaggtccgc cgcatgcaga agctccaggc
     4261 ggagctcctc ttctcggacc actgcgagac ctgctcgggc tgcgcgcgcc acggcgcctg
     4321 cgagctgcag aaggtcgccc gcgtcgtggg ccttgacgtc acgcgccttt cgggccgcct
     4381 cgcaacgcgt cccgtcgaga cggacgcctc ggccaacggc ctcgtcttca cgcccgacaa
     4441 gtgcatccgc tgccttcgct gcatcgaggc ctgccgccag gtgcagggca tcggcgtcat
     4501 caagctcgac catacgggta ccaacgccgc ggtgagcttc ggtggcccct ggggcgagtc
     4561 cgagacctgc atccagtgcg gccagtgcgc gctcgtatgc ccgaccggcg ccctggccgt
     4621 caaggatcag accgactgcg cgctcgactg gttcgacgat cccgcagtca cgaccgtcgt
     4681 gcagttcgcg cccgccgtgc gcgtgacgat cggtgagagc atcggcgcgc gtccgggcga
     4741 aaacctgcag ggcaggatcg tcgccgcgct cagaaagctc ggcgccgatt gcgtgatgga
     4801 tacgcgctgg tccgccgacg tgacgatcat ggaagaggga accgagctgc tcgaacgcct
     4861 tctcaggcag aaggaggagg ggacgctgca cgggcatccc gacacgatgt tcacctcctg
     4921 ctgtccgggc tggatcaacc acatcgagaa gaactgcccc gacatgatcc cgcacatctc
     4981 ctcgacgagg agtccgcagg cgatcttcgg cgcgctcgcc aagacctggc tgccgaagac
     5041 gctcggcatt cccgcggagc gcattcgatc gatctcgatc atgccctgca cggcgaagaa
     5101 ggacgaggcc gcgcgcgagc ttctcaagca cggtggcgaa caggacgtgg atctcgtgct
     5161 cacggtccag gagtttgcgg ccatgctcga ccgtcgcggg atcgacctca tgagcctcgt
     5221 gcccgccgaa ttcgactcgc ccttcatgag cgagggttcg ggcgccgcgc agctcttcgc
     5281 gacgacgggc ggcgtgatgg aggccgcgct caggacggtt tcggcgcttg cgggcgggcc
     5341 tgatctcggc cgtatcgcct tcgagcccgt cagggggctt gcgaccttca aggaggccga
     5401 agtcgagacg gaggccttcg gcaaactgag gatcgcggtc gtgcacggca tgcgtgcggc
     5461 cgacgaggtg atcaggctgg tgcgcgaagg acgctccccc taccacttcg tcgaggtcat
     5521 ggcctgtccc ggcggctgcg tgggcggtgg cggaacggtg cgcggcatcg tctggcgcag
     5581 tacgctcgac cgcaggcaga acgccgtcta cagcaccgac gcctcgatga agttgcgcac
     5641 gtcgcacgaa aacgaggacg tcgtgaggct ctacagggac ttcctcggcg agcccggcag
     5701 tccgcttgcg cacgagcttc tgcattgtga atacagggag cgcgaacgcc gctccgaaaa
     5761 gcccgactac cgcaccatcg agtcggccgt cgagctcgcc agcgtttgat ttctgagcag
     5821 ggcgtccgca ggcgcatccg cctgcgggcc cgtgcataat aagggacaat ccggtaaaaa
     5881 aatccgaatt cattttcatc ctgaatgctt caggatcaag gagaagaaac catgaaagca
     5941 cccgttcgcg tgacggtgac cggccccgcc ggtcagatcg gctacgccac gctcttccgc
     6001 atcgcctccg gcgccatgtt cggcccggac cagcccgtgt tcctcacgct gctcgaacgc
     6061 gccaacccga cctccgccgc cgccctcaag ggcgtgatga tggagctctc cgactgcgcc
     6121 ttcccgcttc tggccggcat gcgcgccgtt caggatcccg tcgaggccat ccgcgactgc
     6181 g
//
LOCUS       DBMDTI010000114         6142 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3255_length_6142_cov_2.713458, whole genome shotgun sequence.
ACCESSION   DBMDTI010000114 DBMDTI010000000
VERSION     DBMDTI010000114.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3255_length_6142_cov_2.713458"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(105..1637)
                     /locus_tag="ACZPB6_06415"
     CDS             complement(105..1637)
                     /locus_tag="ACZPB6_06415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184133.1"
                     /translation="MTEKMLSRRHFLGTAAAAGAAAPAFAASTSASGMPAKWDMETDV
                     VVLGCGGAGMMAACQVVDAGKKVEVFDAGLSPFHTATNLCGGLFTAYGSKLQKADPTI
                     KDSWEAFAEDIMAYGNYMSLKEPVYAFAKHSGEAFDWLSDHGLAAHHLENYAGHTHRR
                     AHRQDSFKGRDYIECLVKQFAERKIEIHHSMPVTRFWFDEKANRVVGVQCGRDDKVVN
                     CRARLGVIMATGGITGTPESLDRWVPSVAGKGVVIGGPNNDGSAMLTAVRDVGVPLSH
                     MQYIASYPCGIVTGGRNGPYCRWWFITNQGGILVNKNGKRFVTEKEGICHVTPHLAHN
                     PDGCHYVLADQATWDRTLKTIKLSALVGLPSWTEERVLKEFELGKNLWKADTLEELCQ
                     KSGMNLEGLKGQIELWNKAVETKNDTQFGRTDQQHKIGAGPYRMIRMFPWNNLSCGGV
                     RVTEHFEVLGWDMKPVNGFYAAGETVAGVHGAFYCGGNACGFAHTSGYMAGKYITGYK
                     EA"
     gene            complement(1640..1975)
                     /locus_tag="ACZPB6_06420"
     CDS             complement(1640..1975)
                     /locus_tag="ACZPB6_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR1184134.1"
                     /translation="MLLKSYLAAVVLTFGLASGAHAAAVTINGPHASLDCTTCHANGQ
                     FKAPAKETCFQCHQSYQTVAERTAKMNPNPHSNHRGEKDCNACHSMHGKSHFECNDCH
                     NFAIRMKGE"
     gene            complement(2150..3865)
                     /locus_tag="ACZPB6_06425"
     CDS             complement(2150..3865)
                     /locus_tag="ACZPB6_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HIR1184135.1"
                     /translation="MGRPLTGKKHVGIRRDTRPNGDVYVYERVTGYDAKTQKTKTIST
                     RLLGKILAGTTEMIPTRPKKSRSEVVEPPVEAVRTHVGLQKILEWAGKESGIDEDLKT
                     CFEIGDAQKLSSIARYWVATDGDTLPRMETWQMMNPLPYEYPITQDVYGKLFASVGRN
                     ESGLQAYFRSRAQRVSSSGLNLALDTTTFSTYSKNQIEARQGFNKDGDGLDTIKLLVL
                     YSVENREPVAFSKQPGNIPDKISLANSLRQLEVLGLSAPVIVADNGFYCQANMTYLAR
                     EHVKFLMLANSTDKWVREEIDAVREEIGAFENSCPFDVGVNAVMRRRQHDFSIVRQRT
                     RGNYEAGQTETFTRRLYVHVCYKPEAAPIQERRLKESLFSIKKDLEDGVEEFRPAAQK
                     LIDNFLIVKRTSKGVKVSFKNEKIEEAKRYWGYFVLVSNEIRDPFEALKAYRSREKIE
                     ELFATYKDSFDGRKPRTWYPENLYGRQFAQFVGLGYHCFLTKRIQDVKKGLAQKSTEK
                     KTKEELNLEEKLLAWLDQHSLIQILDWFRCVDYVATTGNDSASKWTTETTKRDQLFLK
                     LLGVS"
     gene            complement(4224..5723)
                     /locus_tag="ACZPB6_06430"
     CDS             complement(4224..5723)
                     /locus_tag="ACZPB6_06430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184136.1"
                     /translation="MQRRKLLSAAGTLFAAPALFAAPAVEPEAKAASDAAGRTPQDPD
                     DVLIIGAGGAGLAAAVSALESGARRVTILERGAFVGGHTVISAGTVTIATGGETPVED
                     LARDIIEAGGRPELARTLAENAGAAVQWLGSMGVAWEPRLFRAVGSGMSARRNLSTGS
                     QRGGWDMIQALSERARTLGAKTLFETRACRLLLENGRVGGVAARTASGDEVLRYARAV
                     VLATGGFSGNHELRSRFAPRLDSSYGTTADTFGLTPDLARGDGILMGEAAGAQLVDMD
                     AVECIPYAGGRVLDYAGAEVWINAEGDRFVNEEATFDVIAAAMQEQTGGFMWTITDAK
                     SQKGANFRSKLSAGLVREARSLTALAHDIGISIERLSFTMNSYNRMVALGKDPRFGRT
                     TFLQTIDTPPFYYGIERLCVHITMGGLLIDADARVLDRMNAPVPGLFAAGETTGGVHG
                     RKRLGGNALTDAFVFGRIAGAGAARLALGRCPPGRISPHSIRTGSRAPA"
     gene            complement(5710..>6142)
                     /locus_tag="ACZPB6_06435"
     CDS             complement(5710..>6142)
                     /locus_tag="ACZPB6_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1184137.1"
                     /translation="QDKLIEMGCRLPIIFLSAHGSIPTAVSAVQKGAVDFLEKPIEPA
                     ELIAKINAAVARSVTESVETMHRDRLRARFETLTAREREIIGEILKGDGSETNKQIAR
                     TFDLELSTVKMHRSNAFMKLGVHSAAELIRLAQEAGIHAEA"
BASE COUNT         1123 a         1679 c         2029 g         1311 t
ORIGIN      
        1 ctgacccgag cctcaggaag gagccgccca aaggagaaga ggacggcgcc tttttcgagg
       61 agaagctccc tgcgcttaga gcccagagaa tcggctgctg ccggttaggc ttccttgtag
      121 cccgtgatgt acttgccggc catgtagccg gaagtgtggg cgaagccgca ggcgttgccg
      181 ccgcagtaga aggcgccgtg gacgccggcc accgtttccc ccgcagcgta gaagccgttg
      241 acgggcttca tgtcccagcc gaggacttcg aagtgctcgg tgacgcgcac gccgccgcag
      301 gagaggttgt tccacgggaa catgcggatc atgcggtacg gaccggcacc gatcttgtgc
      361 tgctggtcgg tacggccgaa ctgcgtgtcg ttcttcgttt cgacggcctt gttccagagt
      421 tcgatctggc ccttgaggcc ttcgaggttc atgccggact tctggcagag ctcttcaagc
      481 gtgtcggcct tccagaggtt cttgccgagt tcgaattcct tgaggacgcg ttcttcggtc
      541 caggagggca ggccgacaag agcagagagc ttgatcgtct tgagcgtgcg gtcccaggtg
      601 gcctggtcgg cgagcacgta gtggcagccg tccgggttgt gggcgaggtg cggcgtgacg
      661 tggcagatgc cttccttttc cgtcacgaag cgcttgccgt tcttgttgac gaggatgccg
      721 ccctggttgg tgatgaacca ccagcggcag tacgggccgt tgcggccgcc ggtgacgatg
      781 ccgcagggat acgaggcgat gtactgcatg tgggagagcg gaacgccgac gtcgcgcacg
      841 gcggtgagca tggcggagcc gtcgttgttc gggccgccga ttacgacgcc cttgccggcg
      901 acggacggaa cccagcggtc aagagactcg ggcgtgcccg tgatgccgcc cgtggccatg
      961 atgacgccga ggcgggcgcg gcagttgacg accttgtcgt cgcggccgca ctggacgccg
     1021 acaacgcggt tggccttttc atcaaaccag aagcgcgtga cgggcatgga gtgatggatc
     1081 tcgattttgc gttcggcgaa ctgcttcacg aggcattcga tgtagtcgcg ccccttgaag
     1141 gaatcctgac gatgggcgcg gcgatgcgtg tggccggcgt agttttcaag atgatgagcg
     1201 gcaagaccgt ggtcggaaag ccagtcgaag gcttcgccgg agtgcttggc gaaggcgtag
     1261 accggttcct tgaggctcat gtagttgccg taggccatga tgtcttctgc aaaggcctcc
     1321 cagctgtcct tgatggtcgg gtcggccttc tggagcttgg agccgtaggc ggtgaagagg
     1381 ccgccgcaga gattggtggc agtatggaac ggggagaggc cggcgtcgaa gacttcgacc
     1441 ttcttgcctg cgtcgacaac ctggcaggcg gccatcatgc cggcgccgcc gcagccgaga
     1501 acgacgacat cggtttccat gtcccacttg gcgggcatgc cggaggcgga ggtggaggcg
     1561 gcaaaggccg gagcggcggc gcctgcagcg gcggcggtgc cgagaaaatg gcggcgggaa
     1621 agcatctttt cagtcatgat cattcaccct tcatgcggat cgcaaagttg tggcagtcgt
     1681 tgcattcgaa atgggacttg ccgtgcatgc tgtggcaggc attgcaatcc ttttcgccgc
     1741 ggtgattgct gtgcgggttg ggattcatct tggcggtgcg ttctgcgaca gtctgatagg
     1801 actggtggca ctggaagcag gtttccttgg cgggggcctt gaactggccg ttggcgtggc
     1861 aggtcgtgca gtcgagagag gcgtgcggac cattgatcgt gaccgcggcg gcatgagcgc
     1921 cggatgcaag accaaaggtc aggacgacgg ctgccagata agacttcaaa agcatgtcat
     1981 tctccttgtg gggccgcccg gattcatgag gcgcgccgca ggcagtggag gatcagcctg
     2041 acgcgcccgg gggtgaatgc gggcagatgg gttggaagcg ggcggatgat tccgcttcct
     2101 tctgtgccaa atcttaagga tcctaaaagt cgtctaatgc ggccacgatt caactgacac
     2161 caagcagctt gaggaatagc tgatcgcgtt tggttgtttc cgtggtccat ttggaagcac
     2221 tgtcgttacc tgtcgtagca acgtagtcga cgcaacggaa ccaatcaaga atttgaatca
     2281 gtgagtgctg atcgagccaa gcaagcagct tttcttcgag attcaactct tccttcgttt
     2341 tcttttcagt gctcttttgg gcaagcccct ttttcacatc ctgaattctc ttcgtcagga
     2401 agcagtgata gcccagcccg acgaattgtg cgaattgtct gccgtaaagg ttttccggat
     2461 accacgttct gggcttgcgt ccgtcgaagc tgtccttgta ggtcgcgaac aactcttcaa
     2521 tcttttcgcg ggagcggtat gctttcagag cttcaaaggg atcgcgaatt tcgttgctca
     2581 cgaggacaaa gtacccccag tatcgcttgg cttcttcgat cttttcattc ttgaaggaaa
     2641 ccttgacgcc tttgcttgtg cgcttcacga tgaggaagtt gtcgatcagc ttctgggcgg
     2701 caggtcgaaa ttcttcaaca ccatcttcca aatctttctt gattgaaaaa aggctctcct
     2761 taagacgtct ttcttgaatc ggagctgctt ccggcttgta acaaacatga acatacaatc
     2821 gccgggtgaa tgtttcagtc tgtcctgctt cgtagttgcc gcgcgtgcgc tgacgaacga
     2881 tgctgaaatc gtgctggcga cgacgcatga cggcattgac accgacatca aacggacagg
     2941 aattttcgaa agcgccaatc tcttcgcgaa cagcatcaat ttcttcacga acccatttgt
     3001 cggtgctgtt ggcgagcatc aggaacttca cgtgctcccg cgcgaggtaa gtcatgttgg
     3061 cctgacagta aaagccgttg tcagccacga taaccggtgc agagaggccc agcacctcaa
     3121 gctgccgtag agaatttgcc agcgagatct tgtccggaat gtttcccggc tgcttgctga
     3181 aagctaccgg ttctcgattc tcaactgaat acaggaccag aagcttgatc gtatccagtc
     3241 cgtcgccatc cttgttgaat ccctgacgcg cttcgatttg attcttcgaa tatgtagaaa
     3301 aggtcgttgt gtccagagcc aaattcaagc ccgacgagga aactctttga gcacggcttc
     3361 gaaaataggc ttgcaggcct gactcattac gtccgacaga cgcaaaaagc ttgccgtaca
     3421 catcctgcgt gatggggtac tcgtaaggca acggattcat catctgccac gtctccatgc
     3481 gaggcagcgt atcaccatca gtggcaaccc aatatcgggc gattgaactc aatttctgag
     3541 catccccaat ctcgaagcat gtcttcagat cctcatcgat gcccgattcc tttccggccc
     3601 attcgagaat tttctgcagt ccgacatgcg tgcgcacagc ttcaaccgga ggctctacaa
     3661 cctcagaacg cgattttttg gggcgagtcg ggatcatttc cgtcgttccg gccagaattt
     3721 ttccaagaag tcgggtagag atcgtcttgg ttttctgcgt cttggcgtca tatccggtaa
     3781 cgcgttcata cacgtaaaca tcgccattcg ggcgggtgtc acgacgaatt ccaacatgct
     3841 tttttccagt gagaggacgt cccataatct gctccaatgg tgtacctgta ttatattata
     3901 ggtgcaccat tgaagtcaat aaaaaagtcc gtgtgtttca cggactttta gtttgaaaga
     3961 cctactccca acagggtttc gtggggtctt tcgacgactt ttaggttaag gattgctaag
     4021 aaagaaaaat acggctcggg ggaggtggcc gtgggaggtg gtgttaaccc tgaagaaggg
     4081 gcaggagacg ggatttggac cgcctcgaga gcgaggccgc gtcgtcttga aacgactcgg
     4141 ggagagggga ggcgggcgtt ttcgaagggg gcgggcagag actcggggga ggctgaggaa
     4201 tgtcgataag gcagatggga gcgtcacgcg ggtgcgcggc tccctgtgcg gatggagtgc
     4261 gggctgatgc gtccgggggg gcagcggcca agcgcgaggc gtgcggcgcc cgcacccgcg
     4321 atgcgcccga agacgaaggc gtcggtgagg gcgttgccgc cgaggcgctt tcgtccgtgg
     4381 acgccgcccg tggtctcgcc tgcggcgaag aggccgggga cgggggcgtt catgcggtcg
     4441 aggacccggg cgtctgcgtc gatgaggagt ccgcccatcg tgatgtgcac gcacagacgc
     4501 tcgatgccgt agtagaaggg cggcgtgtcg atcgtctgca ggaaggtcgt gcgtccgaaa
     4561 cgcgggtcct tgcccagggc gaccatgcgg ttgtagctgt tcatcgtgaa gctgagcctc
     4621 tcgatcgaga tgccgatgtc gtgggcgagc gcggtgaggg acctggcctc cctcacgagg
     4681 ccggcggaga gcttcgatct gaagttggcg cccttctggg acttggcgtc ggtgatcgtc
     4741 cacatgaagc ccccggtctg ttcctgcatg gcggcggcga tgacgtcgaa ggtggcctcc
     4801 tcgttgacga atctgtcgcc ctcggcattg atccacactt cggccccggc atagtcgagc
     4861 acgcggccgc cggcataggg aatgcactcg acggcgtcca tgtcgacgag ctgcgcgcct
     4921 gcggcttcgc ccatgaggat gccgtctccg cgggcgaggt cgggcgtgag tccgaatgtg
     4981 tccgccgtgg tgccgtagga ggagtcgagt cggggtgcga agcggcttct gagctcgtgg
     5041 ttgccgctga agccgcccgt ggcgagcacg acggctcggg cgtagcggag tacttcgtcg
     5101 cccgaagcgg ttctggcggc gacgccgccg acgcggccgt tctcaagcag aaggcgacag
     5161 gcgcgtgttt cgaagagcgt cttcgcaccg agggtgcggg cgcgctcgga gagcgcctgg
     5221 atcatgtccc agccgcctct ttgagagcct gtggagagat tgcgtcgtgc gctcatgccg
     5281 gagcccaccg ccctgaagag cctgggctcc caggccacgc ccatgctgcc gagccactga
     5341 acggcggcgc ctgcgttttc ggcgagggtg cgtgcgagct cgggtcgtcc gccggcttcg
     5401 atgatgtcgc gtgcgaggtc ttcgacgggc gtctcgccgc cggtggcgat cgtgacggtg
     5461 ccggccgaaa tgacggtgtg tccgcccacg aaggcgccac gctcgagaat ggtgacgcgc
     5521 cgcgcgcccg actcgagcgc ggagaccgcg gcggcaaggc ctgcgccgcc cgcgccgatg
     5581 atgaggacgt cgtcgggatc ctgaggagtg cggcctgcgg cgtcggatgc ggccttcgcc
     5641 tcgggttcga cggcgggcgc ggcgaagagc gccggggcgg cgaataatgt gccggcggcc
     5701 gaaaggagct tacgcctctg catggatccc tgcctcttgg gcgagcctga tgagttcggc
     5761 ggcggaatgc acgccgagct tcatgaaggc gttcgagcgg tgcatcttga cggtgctcag
     5821 ttcaaggtcg aacgttctgg cgatctgctt gttggtctcg ctgccgtcgc ccttgaggat
     5881 ttcgccgatg atctcgcgtt cgcgggcggt gagcgtctcg aagcgcgcgc gaaggcggtc
     5941 tcggtgcatc gtctcgacgg actcggtgac gcttcgtgca acggcggcgt tgatcttcgc
     6001 gatgagttcg gcgggctcga tcggcttttc aagaaagtcg acggcgccct tctgtaccgc
     6061 ggacacggcg gtgggaatgc tgccgtgggc cgaaaggaag atgatcggca gcctgcatcc
     6121 catttcgatg agcttgtcct gg
//
LOCUS       DBMDTI010000115         6096 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3280_length_6096_cov_3.892744, whole genome shotgun sequence.
ACCESSION   DBMDTI010000115 DBMDTI010000000
VERSION     DBMDTI010000115.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3280_length_6096_cov_3.892744"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(168..836)
                     /locus_tag="ACZPB6_06440"
     CDS             complement(168..836)
                     /locus_tag="ACZPB6_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR1184138.1"
                     /translation="MANNRILRFHAPDKVFHSVNAITWFALLFTGVYVYFCEPEAACA
                     EATMLAHLILGVVFTFNLLGFIVLAPDRFALILRACLEWDRNTIFWFRNFGGYPRRFF
                     KIPFGPVEVPPQGRYNGGQKASYLLFMGMIAALTVTGWMLWIGAPLLGKAVFLWTFYF
                     HVWGSIIVSVLVVCAHIPLALLSMEHFKGIWRLGPGTISVEAAEHHAPLWFERDVIRT
                     NIEK"
     gene            complement(829..1185)
                     /locus_tag="ACZPB6_06445"
     CDS             complement(829..1185)
                     /locus_tag="ACZPB6_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HIR1184139.1"
                     /translation="MFSENYDYAERPAALILGRRGFLKVTGLCVAAVAVCGYAIGDLV
                     ARRGVIIKARQAGLYKDDKLCQALGLTSSHQNPTVMQIYKDFGAKPTDHHMHELLHTH
                     YYPRTMLASLTEANHG"
     gene            complement(1208..2566)
                     /locus_tag="ACZPB6_06450"
     CDS             complement(1208..2566)
                     /locus_tag="ACZPB6_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR1184140.1"
                     /translation="MSNNTIKVRTFNPGAGAYGEDGPAYQGNLRKGELRGIIHINKEH
                     CVGCDTCSKFCPVNAIEGGLGAKHHIIEDACLYCGQCLIACPFDAIEQMSFVDQVEKV
                     LADKSRLCVAHPSPAVRVSICEEFGGKPGELTTEQLSNALEAVGFQVYDCNSTADQTI
                     LEEGTEFVKKARYWLLGERSEEVNEMAEHPFPHFTSCCPAWVKNMETNHADLIPHVST
                     AKSPLQMGGALAKTWGAKFLWKCDPRKIFFVSVTPCTAKIFEAARPEMNQGWHWAKEQ
                     GMIPADAPSYQDIDACLTARDLAELFRRKGVNPLKMDKKRERGTLEIYTGAGTIFGVS
                     GGVMEAALRTAYFVLSGEELKNADIEIVRGHNNAIVEATIPVPIKAKGGQTVDIRICV
                     VNGANQGLEEVLHRVRLDKNRYHFIEVMNCPGGCVNGGGQPVQPVGTAWLNPTLPLPL
                     RA"
     gene            complement(3031..4383)
                     /locus_tag="ACZPB6_06455"
     CDS             complement(3031..4383)
                     /locus_tag="ACZPB6_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HIR1184141.1"
                     /translation="MAQPKFSAIPTHMSTTSYKPQPRELLPEWMIGMTDRIMDFYTKR
                     YLHCDPVILKNPPPPQDGHKYLLYAHIPFCHTLCSYCTFHRFLFKEHVARAYFVNLRK
                     EMDYVKALGYDFHSMYVGGGTTTIIEEELIKTLEHARKLFPSIKEISCETDPQIITTP
                     QFRNLKGLVDRMSIGVQSFNDDLLKLSERYEKFGSGEQIFERLQYAQELFPTCNIDMM
                     FGFRGQTLDMLHEDMEKIWKLNPRQVTTYPLMVTSQTRKSVKNVIAASGNQLADQYRV
                     ILDTLGSRYNHLTSWTFGRTHDEGFDEYVVDYDEYLGVGSGAFSFLNDSLYVNTFSLR
                     RYNERIAAGNTGVERRRQFDKHAILQYRLMLGLFSHRLSRKYFREVHGVNLDRTLMKE
                     MLALRVAGAIKDDPNDPDNIIVTDGGKFLGLVMMKAFYSAMDNVRAELRQPLKEEDYS
                     "
     gene            complement(4462..>6096)
                     /locus_tag="ACZPB6_06460"
     CDS             complement(4462..>6096)
                     /locus_tag="ACZPB6_06460"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR1184142.1"
                     /translation="AAMNLERGTRIGILMPNSIDHACADQAALANGLVPVPLHAIDTP
                     GASAFILSDSRAKVLVTNKLTRWKQIKAAGGDLQDLTAVIITEDEVDDETGMVRGLSE
                     WLAAGTHVVELPEGPKEDDLAGIVYTSGTTGRPKGVMLTHGNIVSNVKATLECVFPQV
                     GDIFLSFLPLSHTFERTAGYYLALATGCTIAYNRSVLLLADDLKTIRPTVIISVPRVY
                     ERIFARVHDKLKKSRPAARYLFDWAVEIGWRDFCRRNHLPVEHTGRAWLDGLMRPLVR
                     KVSSTLLDQFGGRLRIAISGGAALSSKVARTFCGLGLPIIQGYGMTEASPIIAGNNRT
                     LNQPNTVGKPFNNVEVRLGEGDEIQIKGPSITRGYWNRPDATADAFTEDGWFRTGDVG
                     GFNELGLLSIKGRIKEIIVTSTGEKVPPADLEAAIETDPLFSQCYVIGENRPYLSLIT
                     VVNPDEWASFAKSCGVDPADPASLDNPSVKTAALKRAKTAAGEFPHYALPRAVVLTQE
                     PWTIENGLITPTLKLKRGPLSKKFANVIAQLYATHG"
BASE COUNT         1175 a         1607 c         2079 g         1235 t
ORIGIN      
        1 gccgatgtga aggcgaaggc ctttgggggt ttcaaccatg atttttttca agtccgcagg
       61 ggcaaaaaaa gaccggacaa gcatgcttat ccggtcttgt cggagaagca gtccggagga
      121 ctgccttccg ttatatcaca ttgcagatca ggcgatccgc gtgattttta cttttcaatg
      181 ttggtgcgga tgacgtcacg ttcgaaccag agcggagcgt gatgttcagc agcttcaaca
      241 gagatggtgc ccgggccgag gcgccagatg cccttgaagt gctccatgga gagcagagca
      301 agcgggatgt gggcgcagac gaccagcacg gagacgatga tggaacccca gacatggaag
      361 tagaacgtcc agaggaacac ggccttgccg aggagcggag caccgatcca gagcatccag
      421 cccgtgaccg tcagggcggc aatcatgccc atgaacagga ggtaggaagc cttctgaccg
      481 ccgttgtagc gaccctgcgg cggaacttcc accgggccga acgggatctt gaagaagcgg
      541 cgcggatagc cgccgaagtt gcggaaccag aagatcgtgt tgcggtccca ttcaaggcag
      601 gcgcggagaa tgagggcaaa acggtccgga gcgagcacga tgaagcccag aaggttgaac
      661 gtgaacacga cgcccaggat caggtgagcg agcatcgtgg cttcagcgca ggcggcttcc
      721 ggttcgcaga agtagacgta aacgccggtg aagagaaggg cgaaccacgt gatcgcgttg
      781 accgagtgga acaccttgtc aggggcgtgg aaacgaagaa tgcgattgtt agccatggtt
      841 ggcctccgtc agggacgcga gcatcgtgcg cggatagtaa tgggtgtgga gcagttcgtg
      901 catgtggtga tcggtgggct tggcaccgaa gtccttgtag atctgcatca cggtcgggtt
      961 ctggtgggag gacgtcagac cgagagcctg gcagagcttg tcgtccttgt agagaccagc
     1021 ctgacgggcc ttgatgatca caccacgacg ggcaacgagg tcgccgatgg cgtagccgca
     1081 gacagcaacg gcggcaacgc agagaccggt gaccttaaga aaaccgcggc gacccaggat
     1141 caaggcagca ggacgctcgg cgtagtcgta attttcagag aacattatct aattccttgc
     1201 gaaaaaatta ggcacgcagc gggagcggaa gcgtcgggtt gagccaagcg gtgccgaccg
     1261 gctggaccgg ctggccgccg ccgttgacac agccgcccgg gcagttcatc acttcgatga
     1321 agtggtagcg gttcttgtcg aggcgaacgc gatggaggac ttcctcgaga ccctggttgg
     1381 cgccgttgac aacgcagatg cggatgtcga cggtctggcc gcccttggcc ttgatgggaa
     1441 cggggatcgt ggcttcaacg atggcgttgt tgtggccgcg gacgatttcg atgtcggcgt
     1501 tcttgagctc ttcgccggag aggacgaagt aggccgtacg cagggcagct tccataacgc
     1561 cgccggaaac gccgaagatc gtgccggcgc cggtgtagat ttcgagcgtg ccgcgttcgc
     1621 gcttcttgtc catcttgagc gggttgacgc ccttgcgacg gaagagttcg gcgagatcac
     1681 gagccgtaag gcaggcgtcg atgtcctgat aggacggagc gtcggccggg atcatgccct
     1741 gttccttggc ccagtgccag ccctggttca tttccggacg agcggcttcg aagatcttgg
     1801 ccgtgcacgg cgtgacggag acgaagaaga tcttgcgcgg gtcgcacttc cagaggaact
     1861 tggcacccca ggtcttggca agggcgccgc ccatctggag cggggacttg gcggtggaga
     1921 cgtgcgggat caggtcggca tggttcgttt ccatgttctt gacccaagcc gggcagcagg
     1981 acgtgaagtg cgggaacgga tgttcggcca tttcgttcac ttcttcgctg cgttcgccga
     2041 ggagccagta gcgggccttc ttgacgaatt ccgtaccttc ttcaaggatc gtctggtcag
     2101 ccgtgctgtt gcagtcgtag acctggaagc cgacggcttc gagggcgttg gaaagctgtt
     2161 ccgtagtgag ttcgccgggc ttgccgccga attcttcgca gatggagacg cggacggcgg
     2221 gagacggatg ggcgacgcag aggcgggact tgtcggcgag aaccttttcg acctggtcga
     2281 caaagctcat ctgttcgatg gcgtcgaacg ggcaggcgat caggcactgg ccgcagtaaa
     2341 ggcaggcgtc ttcgatgatg tggtgcttgg caccgaggcc gccttcaatg gcgttgacag
     2401 ggcagaactt ggagcacgtg tcgcagccga cgcagtgttc cttgttgatg tggatgatgc
     2461 cgcggagttc tccctttcgg agattaccct ggtaggcggg tccgtcctcc ccgtaggcgc
     2521 cggcgccggg gttgaacgta cgaaccttga tcgtattgtt ggacattgaa aaactctcca
     2581 gaatcagcgg aactcaggaa gctccttgcc ttgcacgtcc ttgccagaga ctgtgagacg
     2641 cagtgctcgg gggatgacaa gaacggtttc gggcgtgctg aattttttga aacgaaagcg
     2701 acgggtacag caagacccgg gcgctcacgg tgaaacacgc ttcgcatttt aaggggttaa
     2761 ccgctaatgt aatctaccta aaagtagtag tcgctaatcc tgatagggta gtttttgacg
     2821 aaaacgtagg aaaaaccgca aatcgttaac atttgtcaat cggcgttcgg tcttgcttac
     2881 actcagtcta atttaagcct tcaagaggcc tgtgccgtgg agctttcggt cgtttgaaac
     2941 agaagggccc cgagggattt tcatccttcg gggcctttgt tcaaagggat tcgcgaagat
     3001 cgggctctga gggcggtcga ttcagccgga tcaggagtag tcctcttcct tcaggggctg
     3061 gcgcagttcg gcgcgaacgt tgtccatggc cgagtagaag gccttcatca tgacgaggcc
     3121 gaggaacttg ccgccgtcgg tcacgatgat gttgtccgga tcgttcgggt cgtccttgat
     3181 ggcgcccgcc acgcgcagcg cgagcatttc cttcatgagc gtacggtcga gattcacgcc
     3241 gtgcacttcg cggaagtact tgcgggacaa gcggtgggag aacaagccga gcatcagtcg
     3301 gtactggagg atcgcgtgct tgtcgaactg gcggcggcgt tccacgcccg tgttgccggc
     3361 ggcgattcgc tcgttgtagc ggcgcaggct gaacgtattg acgtagaggc tgtcgttgag
     3421 gaagctgaac gcgccggaac ccacgccgag gtactcgtca tagtcgacca cgtattcgtc
     3481 gaagccttcg tcgtgcgtac ggccgaacgt ccaggacgtc aggtggttgt agcgcgagcc
     3541 gagcgtgtcg agaatgacgc ggtactggtc ggcaagctgg ttgccgctgg cggcgatgac
     3601 gttcttcacg ctcttgcggg tctgggacgt gaccatgagc gggtaggtcg tgacctgacg
     3661 cggattgagc ttccagatct tctccatgtc ttcgtgcagc atgtcgagcg tctggccgcg
     3721 gaagccgaac atcatgtcga tgttgcaggt cgggaagagt tcctgggcat actgcaggcg
     3781 ctcgaagatc tgttcgcctg agccgaactt ctcatagcgt tcggaaagct tgagcaggtc
     3841 gtcgttgaag ctctgcacgc cgatcgacat gcggtcgacg aggcccttca ggttgcggaa
     3901 ctgcggcgtc gtgatgatct gagggtcggt ttcgcaggaa atttccttga tcgacgggaa
     3961 aagcttgcgg gcgtgctcga gcgtcttgat gagctcttcc tcgatgatgg tggtcgtgcc
     4021 gccgccgacg tacatggaat ggaagtcata gccgagggcc ttgacgtagt ccatttcctt
     4081 gcgaagattc acgaagtacg cgcgcgccac atgctccttg aagaggaagc ggtggaaggt
     4141 gcagtacgag cacagggtgt ggcagaacgg aatgtgtgcg tacagcaggt acttgtgtcc
     4201 gtcctgtgga ggtggcggat tcttgagaat gacggggtcg cagtgcaggt agcgcttcgt
     4261 gtagaagtcc atgatgcggt ccgtcatgcc gatcatccat tccggcagaa gttccctggg
     4321 ctggggtttg tagcttgtcg tgctcatgtg tgtgggaata gctgaaaact ttggttgcgc
     4381 cattgtacaa gaggcgggga gggcgcaggc ttaagaggcg tgcctgcaaa gcacgcctcc
     4441 cgactgccgc cgtctgcagg atcagccgtg cgtggcgtag agctgcgcga tgacgttcgc
     4501 gaatttcttt gagagcggac cgcgtttgag cttgagagtc ggcgtgatga ggccgttttc
     4561 gatcgtccaa ggctcctgcg tgagcacgac ggcgcgcggc agcgcgtaat gcgggaattc
     4621 gcccgccgcc gtcttggcgc gcttgagggc cgcagtcttc acggaaggat tgtcgaggct
     4681 ggcgggatcg gcgggatcga cgccgcagga cttcgcaaac gaggcccatt catcgggatt
     4741 cacgacggtg atgaggctca ggtacggacg gttttcgccg atgacgtagc actgggagaa
     4801 gaggggatcg gtttcgatcg cggcctcaag gtcggcgggc ggcacctttt cgcccgtact
     4861 cgtcacgatg atttccttga tgcgtccctt gatgctcaga aggccgagtt cattgaagcc
     4921 gcccacgtcg cccgtacgga accagccgtc ttccgtaaag gcgtcggcgg tggcgtcggg
     4981 tcggttccag tagccgcgag tgatgctcgg ccccttgatc tggatctcat ccccctcgcc
     5041 gaggcgaacc tcgacgttgt tgaagggctt gcccacagtg ttgggctgat tgagcgtgcg
     5101 gttgttgccc gcgatgatcg ggctcgcttc cgtcatgccg tagccctgaa tgatgggaag
     5161 gccgaggccg cagaacgtgc gggcgacctt gctgctgagg gcggcgccgc cggaaatggc
     5221 aatgcggagc ctcccgccga attggtcgag cagcgtggac gagaccttgc gcacgagagg
     5281 acgcatcagg ccgtcgagcc aggccctgcc cgtgtgctcc acgggaagat gattgcggcg
     5341 gcagaagtcg cgccagccga tttcaacggc ccagtcgaag aggtagcggg ccgcaggcct
     5401 ggacttcttg agcttgtcat gaacgcgagc gaagatgcgc tcgtagacgc ggggcacgga
     5461 gatgatcacc gtcgggcgga tcgtcttgag atcgtcggcc agaagcagga ccgagcggtt
     5521 gtaggcgatg gtgcagcccg tggcaagcgc gagatagtag cctgccgttc gctcgaacgt
     5581 gtgagaaagc ggcagaaagg agaggaagat gtcgcccacc tgcgggaaga cgcactcgag
     5641 cgtggccttc acgttgctca cgatgttgcc gtgcgtgagc atgacgccct tggggcggcc
     5701 cgtggtgccc gacgtgtaga cgatgcccgc gaggtcgtcc tccttcgggc cttcaggcag
     5761 ctcgacgacg tgcgtgcctg cggcaagcca ctcggagagg cctctgacca tgccggtctc
     5821 gtcgtcgacc tcgtcttcgg tgatgatcac ggccgtcagg tcctgaaggt cgccgcctgc
     5881 ggccttgatc tgcttccagc gcgtcagctt gttcgtgacg aggaccttcg ccctgctgtc
     5941 ggaaagaatg aaggcgctgg cgcccggcgt gtcgatagcg tgcaggggca cggggacgag
     6001 gccgttggcc agagccgcct gatcggcgca ggcatggtcg atgctgttgg gcatcaggat
     6061 gccgatgcgg gtgccgcgct caaggttcat ggcggc
//
LOCUS       DBMDTI010000116         6084 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3289_length_6084_cov_2.319071, whole genome shotgun sequence.
ACCESSION   DBMDTI010000116 DBMDTI010000000
VERSION     DBMDTI010000116.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3289_length_6084_cov_2.319071"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="ACZPB6_06465"
     CDS             complement(<1..378)
                     /locus_tag="ACZPB6_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HIR1184143.1"
                     /translation="MPRPILIKNAEVFAPEKLGRRDIFIAGGRIVAMEESLEGLSVPG
                     LETIDACGAIVTPGLIDQHIHVTGGGGEGGWKSRCPELVFSELVKAGVTTFLGVSGTD
                     SMSRSIENLLAKVRGLKQEGASGW"
     gene            complement(515..1000)
                     /locus_tag="ACZPB6_06470"
     CDS             complement(515..1000)
                     /locus_tag="ACZPB6_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184144.1"
                     /translation="MKPVIGSLMLLLVFLMVIHSFWKWSRQFPPAHEREPSGVEGGLI
                     VLVGLMAAAVLFNATGFGLMVSAVMGAVPEGFWIRTGLPGILSFAGLVWAMLRLLSGR
                     TPSVRLEASLGCLASGPVAEAWMLQADTGDVSRLAVSVVLTAGAVLYLFLSPRSKHTY
                     G"
     gene            complement(1000..1605)
                     /locus_tag="ACZPB6_06475"
     CDS             complement(1000..1605)
                     /locus_tag="ACZPB6_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184145.1"
                     /translation="MMETTVVHFSGMQVMLMIALCAIAVLVPVWAIRRIARAVPPVYQ
                     VPGIPSGVGGLLLFTIVLLIVEAVNALYHFGRAAGEAARVISMSTDYLWPVAQTLIPD
                     FAASFFLLIAIGALVFGRSSVALGAAVVCAWLGGPLVAVLRTIYLGLPIELAGEPTGL
                     LFLTVVVTLYLLFSNRPALTYGTASGRRLALSRGGSAVGER"
     gene            complement(1722..2150)
                     /locus_tag="ACZPB6_06480"
     CDS             complement(1722..2150)
                     /locus_tag="ACZPB6_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1184146.1"
                     /translation="MKIGELARRTLHTAETIRYYERMGLLPAPRRFANNYRDYGEVHV
                     GRLEFIRHCRTLDISLDEIRSLLESIEDGSPEGADRAHVMIHRHLEAVEARMAELRRL
                     REQLCSLAGACCGRHAEGELCGLLERLSHPDAAEDGPDVP"
     gene            2277..4646
                     /locus_tag="ACZPB6_06485"
     CDS             2277..4646
                     /locus_tag="ACZPB6_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR1184147.1"
                     /translation="MSTLIISISAMCCPAEGGPVEQVLRAAPGVADVTLDYSTRTARV
                     TGDRISGEALLEALLSIGMPGELVVTGARRIVLSVPEMDCPVEADEIQKAFAAAGITG
                     ADFNVMTRTVTLSGDDDLEAAAVRAVESCGYTAKAAASGPRTIESAPIPWALYITALF
                     VAFMSEAVELVSEYSPGLIPLEHGTIDLITLALAVVAILMAGLEVFKNGVLALVHRNL
                     NMNALMAVAVVGGVLIGAWPEAAMVMVLFQISESIEQLSMTKARRSIRDLMSAAPETA
                     EVKDGRSWRRVPSSDVGAGAVVRVGPGDRVPLDGRIVRGATALDQSMVTGEGMPAEKS
                     EGDNVWSGTVNLTSTIEVMVTAAASESLSARIIDAVENAQASKSPVQRFVDRFAAVYT
                     PIVFFIAAAVAIVPGLVTGEWSTWIYRALCLLVISCPCALVISTPVTVVSALATATRC
                     GLLIKGGLYLEEARKLVNIGLDKTGTLTKGEPAVAGVKYLATFDERLTGAMAASLAAM
                     NKHPLSQAIARWAHERHLPVYQVEGFTAIPGAGVEGRIERGMVRLVNLRWLEEQGLAD
                     EEVRRTFAHYTNEGMSAVAVADAFGVQAVFGLADVVKKDTVEGLRQLASVGIRPWLLT
                     GDNEAAARALAEKVGLKDVRADLLPEDKLKAIEELQKSGLTAMAGDGINDAPALARAD
                     IGIAMGVRGTDSAIEAAHIAVMDDRISSIATLVRLSRITHGLLVENIAFAIGVKIIFA
                     LLALTGNATMWMAVFADTGTCLIVVANAMRMLRMKPRLDRMAEEARRAA"
     gene            5661..>6084
                     /locus_tag="ACZPB6_06490"
     CDS             5661..>6084
                     /locus_tag="ACZPB6_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184148.1"
                     /translation="MTDPTRLEPRNGDFVAYVEALQEESLEALRRANQAAIANPLQVP
                     DASEGGADLGRIAAEMQRRARAERGDEATHDAAQADMLRRTKKSVAESFRRMGGATRG
                     STRAPAPQPHSPAPAPAAGRAPQARRRGVPLGFIGSACI"
BASE COUNT         1114 a         2033 c         1971 g          966 t
ORIGIN      
        1 ccagcccgag gcgccttcct gcttgaggcc acgcaccttg gcgaggaggt tctcgatcga
       61 gcgggacatg ctgtccgtgc ccgagacgcc gaggaaggtc gtcacgccgg ccttgacgag
      121 ctcggagaag acgagctcgg ggcagcggct cttccagccg ccttcaccgc cgccgcccgt
      181 gacgtggatg tgctggtcga tgagacccgg ggtcacgatg gcgccgcagg cgtcgatcgt
      241 ctcgaggccg gggacggaaa ggccctcaag gctctcctcc atggcgacga tcctgccgcc
      301 tgcgatgaag atgtcgcgac ggccgagctt ttcgggggcg aagacttcgg cgttcttgat
      361 gaggatggga cggggcattc tttctctcct ttggtcctgc ccgcggccga aagtgaaagg
      421 gatggggccg cgcgcgcaat gaggcaattc tacctgcccg acgcctgcag gaagccccgt
      481 gcccgaacgc ctgaaaaccc tcaggggttt acccttaccc gtaggtgtgc ttcgaacgag
      541 gagacagaaa gagatacagg acggcgcccg ccgtaaggac gacggaaacg gcgaggcggg
      601 agacgtcgcc cgtgtccgcc tgcagcatcc aggcctcggc gacggggcct gaggccagac
      661 acccgagcga ggcttcaaga cgaacggagg gcgtgcggcc cgaaagcagg cggagcatcg
      721 cccagacgag gcctgcgaag ctcaggattc ccggcagccc cgtccttatc cagaagccct
      781 cgggcaccgc gcccatgacg gctgagacca tgaggccgaa ccccgtcgca ttgaagagga
      841 cggcggcggc catgaggccc acgagcacga tgaggccgcc ctcgacaccc gagggctcgc
      901 gctcgtgcgc aggcggaaac tgacggctcc acttccagaa ggagtggatc accatcagga
      961 agaccagcag gagcatcagg ctcccgatca cgggcttcat cagcgctccc cgacggcgga
     1021 gccgccgcgc gagagcgcaa gcctgcggcc cgaagcggtg ccgtaggtga gtgcgggacg
     1081 attggaaaag agaagataga gggtcacgac caccgtgagg aagaggagcc ccgtgggctc
     1141 gccagcaagc tcgatcggaa ggccgaggta gatcgtgcgc aggacggcga cgagcgggcc
     1201 gccgagccag gcgcagacga cggcggcgcc caaggcgacg gacgagcggc cgaagacgag
     1261 cgcgccgatc gcgatcagca ggaagaacga ggcggcaaag tccggaatga gcgtctgcgc
     1321 gacgggccag agatagtcgg tgctcatcga gatgacgcgc gcggcctcgc ccgcggcgcg
     1381 gccgaagtga tagagcgcgt tgacggcctc gacgatgaga agcacgattg tgaagagcag
     1441 caggccgccg accccgctcg gaatgcccgg cacctgatag acgggcggaa cggcgcgagc
     1501 gatgcggcga atcgcccaga cgggcacgag aacggcgatc gcacagagcg cgatcatcag
     1561 catgacctgc atgccggaaa agtggaccac ggtggtttcc atcatggttt tcgactccag
     1621 aaaagaggct cctcaagcgg agcacagcgg aggcattcta cacaaggaca ttcgtccgaa
     1681 ctgccttccg cacagggcgt tcaaagattt ttgctttttt ctcagggcac gtccggaccg
     1741 tcttctgcgg cgtcaggatg cgaaaggcgc tcaagcaggc cacagagctc gccctcggcg
     1801 tggcgcccgc agcaggcgcc cgcgagcgag cagagctgct cgcgcagccg cctgagctcc
     1861 gccatgcgcg cctcgaccgc ctcgaggtgc ctgtggatca tcacgtgcgc gcggtcggcc
     1921 ccctcgggac tcccgtcctc aatgctctcg agcagcgagc ggatctcgtc gagactgatg
     1981 tcgagcgtgc ggcagtgcct gatgaactcg agcctgccga catggacctc gccgtagtcg
     2041 cggtagttgt tcgcaaaacg cctgggcgcg ggcagaagtc ccatccgctc gtagtagcgg
     2101 atcgtctccg ccgtgtgcag cgtgcgcctg gccagttcgc cgatcttcat gactcgtccc
     2161 tgaaaaaata atgctgattc gcattatcgc aaaaaaacgc ttgaccttgt agcgacttca
     2221 gggtgtgtaa tgccatccat cagtaattca cgacggacct cgtccgcaga acaatcatgt
     2281 ccactctgat catctccatc tccgccatgt gctgccccgc cgagggcggt cccgtcgagc
     2341 aggtcctcag ggccgctccg ggcgtggcgg acgtcacgct cgactactcc acccgcacgg
     2401 ccagggtcac gggcgacagg atctcgggcg aagccctgct tgaggcgctc ctctccatcg
     2461 gcatgcccgg cgaacttgtc gtcacgggcg caaggcgcat cgtgctctcc gtccccgaaa
     2521 tggactgccc cgtcgaggcg gacgagattc agaaggcctt tgcggccgca gggatcacgg
     2581 gcgcggactt caacgtgatg acccgcaccg tcacgctctc gggcgacgac gatctcgagg
     2641 ccgccgcagt gcgcgccgtc gaatcctgcg gctacacggc aaaggccgca gcctcgggcc
     2701 ccaggacgat cgaaagcgcg ccgattccct gggcgctcta catcaccgcc ctcttcgtcg
     2761 ccttcatgtc cgaagcggtc gagcttgtga gtgaatactc gcccggactc atcccgctcg
     2821 agcacggcac gatcgacctc atcacgctcg cacttgccgt cgtcgctatc ctgatggcgg
     2881 ggctcgaggt cttcaaaaac ggcgttctgg cgctcgttca ccgcaacctc aacatgaacg
     2941 ccctgatggc ggtggccgtc gtgggcggcg tgctcatcgg cgcctggccc gaagccgcca
     3001 tggtgatggt gctcttccag atttcagagt cgatcgagca gctctcgatg acgaaggccc
     3061 gccgctccat ccgagacctg atgagtgccg cccccgagac ggccgaggtc aaggacggcc
     3121 gtagctggcg cagggtcccc tcgtccgatg tgggcgcggg cgccgtcgtg cgcgtgggac
     3181 cgggcgaccg cgtgccgctt gacggcagga tcgtcagggg cgcgaccgcg ctcgaccagt
     3241 ccatggtgac gggcgaaggc atgcccgccg agaagtccga gggcgacaac gtctggtcgg
     3301 gcacggtgaa cctcacctcc acgatcgagg tgatggtgac tgcggctgcg agcgagagcc
     3361 tctcggcccg catcatcgac gccgtcgaga acgcccaagc ctcgaagtct cccgtgcagc
     3421 gcttcgtcga ccgctttgca gccgtctata cgccgatcgt cttcttcatc gcggccgccg
     3481 tcgcaatcgt gcctggcctc gtgacgggcg agtggagcac gtggatctac cgcgcgctct
     3541 gcctgctcgt catctcctgc ccctgcgcgc tcgtgatctc gacgcccgta accgtcgtct
     3601 ctgcgctcgc cacggccacg cgctgcggcc tgctcatcaa gggcggactc tatcttgagg
     3661 aggcgcgcaa gctcgtcaac atcggcctcg acaagaccgg cacgctcacg aagggcgagc
     3721 ccgccgtcgc aggcgtgaag tacctcgcca ccttcgacga aaggctcacg ggcgcgatgg
     3781 ccgccagcct cgccgccatg aacaagcatc cgctctccca ggccatcgca cgctgggcgc
     3841 atgaaaggca cctgcccgtc tatcaggtcg agggcttcac ggccatcccg ggcgcaggcg
     3901 tcgagggccg catcgagcgc ggcatggtga ggctcgtcaa cctgcgctgg ctcgaggaac
     3961 agggccttgc cgacgaggag gtccgtcgca cgttcgcgca ctatacgaac gagggcatgt
     4021 ccgccgtcgc cgtcgccgac gccttcggcg tgcaggcggt cttcggactc gccgacgtcg
     4081 tgaagaagga cacggtcgag ggcctgcgtc agctcgcctc cgtgggcatc cgcccgtggc
     4141 tcctcacagg cgacaacgag gcggccgccc gcgcgctcgc cgaaaaggtc ggtctcaagg
     4201 acgtcagggc ggacctgctg cccgaggaca agctcaaggc catcgaggag cttcagaaga
     4261 gcggcctcac cgccatggcg ggcgacggca tcaacgacgc ccccgcgctt gcccgagccg
     4321 acatcggcat cgccatgggc gtgcgcggca cggacagcgc catcgaggcc gcccacattg
     4381 ccgtgatgga cgacaggatc tcgtcgatcg caacgctcgt gcgcctctcc cgcatcaccc
     4441 acggcttgct cgtcgagaac atcgccttcg ccatcggcgt caagatcatc ttcgcgctcc
     4501 tcgcgctcac gggcaacgcc accatgtgga tggccgtctt cgccgacacg ggcacctgcc
     4561 tgatcgtggt cgccaacgcc atgcgcatgc tgcgcatgaa gccaaggctc gaccgcatgg
     4621 ccgaggaggc ccgcagggcc gcctgacgcg gcccccgcac gcatccggct tcaagcgcct
     4681 ccggcattcc cggaggcgcc acgtccaaca gaaagccgcc gccgagtcct gaggatcaag
     4741 gagaccgcgg ccctctcaac aacaagcgcc cgtcggcatc gggccaaaac gggcactctt
     4801 catagcttcg ggaagaggcg gggtgctcag aggtcgcagg tccagccgtt ttcgcggtct
     4861 cgggccttga tctggagctt gtccttgtac atggccgagt cgtcgcgctt ctgcgtgtcg
     4921 gaaagctcgc ggtcctgagc gccgtcgaag gaggctcagc ccatggcgag ggagacgggc
     4981 acctcgaccg ccttgccgaa gggctcgaag gcggtcttca cgcggcgggc gaggtcggcc
     5041 gcgtcggcgt ccctcgagac gaaaagcacg acgccgagca ggatcgaaac ctgccggtcg
     5101 tctcacgaga aaatggggcg ggacatggaa ggaagtgcgg ggtttaatgt ttggctcttc
     5161 gcagctttga gtgggtaagt gcatggctaa gtggaatcat tccgtcaggg gctccgcccc
     5221 cgacaccccg gcgttcgccg ccacgtggct cgagggaaag gcgggcgtta acgcccttac
     5281 tttccccgaa ccacaggcta tttgcgctga atcacctcgt caatctgctt tcgcggcata
     5341 tgctcggcct aacggcctgc gctattccac gacagcttga cgattcgatt ccgcgcgaga
     5401 attgcccaca cgaatctgcg aaaggcctaa tgtttatttt ttcgttggtc ggcagttctt
     5461 tccttccggc atcaatactg tccaaaagtt gacggcgcga cagaggcttt ccggggtgaa
     5521 gagcctcatt tttgtcggtc taacattata gcggccgcat gggcgcctgt atatagcgtt
     5581 tccaatcgaa aacgctctcg gcgagtagct ttccgacgcg ctatccttgt atggacaccc
     5641 accatttcca atcgcccgcc atgaccgatc ccacaaggct tgaacccaga aacggcgact
     5701 tcgtcgccta cgtcgaggcg ctccaggagg agtcgctcga ggcgcttcgc cgcgccaatc
     5761 aggctgcaat cgcaaatccc ctgcaggtgc ccgacgcctc cgagggcggg gccgacctgg
     5821 gcaggatcgc ggccgaaatg caacgcaggg cccgcgccga gcgcggggac gaggcaacgc
     5881 atgatgccgc gcaggcggac atgctgcgaa ggacgaagaa atccgtggcg gagagcttcc
     5941 gccgcatggg cggcgcaacc cgcggaagca cccgggcgcc cgctcctcag cctcactctc
     6001 ccgccccggc acccgccgca gggcgcgctc ctcaggcgcg cagacggggc gtgccgctcg
     6061 gcttcatcgg ctccgcctgc atca
//
LOCUS       DBMDTI010000117         5976 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3343_length_5976_cov_4.231830, whole genome shotgun sequence.
ACCESSION   DBMDTI010000117 DBMDTI010000000
VERSION     DBMDTI010000117.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3343_length_5976_cov_4.231830"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            135..1367
                     /gene="hydF"
                     /locus_tag="ACZPB6_06495"
     CDS             135..1367
                     /gene="hydF"
                     /locus_tag="ACZPB6_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HIR1184149.1"
                     /translation="MVETPKGLRLHIGLFGKRNAGKSSLINALTNQKISIVSEVAGTT
                     TDPVEKACELAPIGPVVFIDTAGVDDVGALGLARVERTRAVLEWVDIALVVANARDFD
                     ECESDLVEHLRKLGTPAVLVINKSDLHPADEAFMTRMRAYGIPVVLTDAETKAGIADL
                     RKAIIDLAANRIPKDRALVGDLVQAGDTVVLVTPIDTGAPKGRLILPQVQAIRELLDA
                     HAKCLVVQQDRVAESIAELRHPPKLVMADSQVIMDVAAQTPEDIPLTTFSIQMAYSKA
                     DVIEMARGTAALASLEDGDRVLICETCSHHPQKDDIGRIKIPRWLRQKTGKDLQIEVA
                     VGKDFPVDLTPYKVLIQCGGCVVTRRHMLMRLRAAHAQNVPMTNYGLTISYLQGVLER
                     VLLCHPEALKAYRDALTK"
     gene            complement(1440..3044)
                     /locus_tag="ACZPB6_06500"
     CDS             complement(1440..3044)
                     /locus_tag="ACZPB6_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR1184150.1"
                     /translation="MTSPNPALVSDVEMRRTFAIISHPDAGKTTLTEKLLLFGGAIQM
                     AGAVKGRKAARHATSDWMEVEKQRGISVTSSVMQFHYDNHVINLLDTPGHEDFSEDTY
                     RVLTAVDAAVMVIDAAKGVEAQTIKLLEVCRMRKTPIITFINKLDREVRDPLDVIGEI
                     ENVLKLQCVPITWPIGMGKTFRGVFSLLKNRLVRFTAGEERLSRDQEMIEGLDNPELD
                     RLFPMEMPYVRESVELVQGATEPFDIEKFLAGEQSPVFFGSGVNNFGVQDVLDALVQW
                     APPPQPRDAGVRKIEPTEAPFSGFVFKIQANMDPRHRDRIAFFRVCSGRYSQGMKVFH
                     VRENRDMKIPNALTFMAQDRVVMTDAVAGDIIGIYNHGQLHIGDTLTEGEKLGFTGIP
                     SFAPELFRAARSKDPFKAKQLQKGLQELGEEGAIQVFTGEFGNILLGAVGQLQFEIVA
                     QRLATEYKVDAIYESTDVSTARWLSFPDEQTRKDFCKEQDARLATDVNGNTVYLATSI
                     YNLETTMKHWPQVVFHTTREMQQTFD"
     gene            complement(3170..5431)
                     /locus_tag="ACZPB6_06505"
     CDS             complement(3170..5431)
                     /locus_tag="ACZPB6_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HIR1184151.1"
                     /translation="MTTTEQIIFEIIAADIQARPAQVAAAVGLLDDGCTVPFVARYRK
                     EVTGGLDDTQLRLLEERLGYLRELEKRREVILGSIRDQGKLTPELEAKIVAADTKQGL
                     EDLYLPYRPKRRTLAAIAREAGLEPLAFKLFENPGLDPETEAVAFVSPEKNVPDVKAA
                     LNGARDILAEKMSEDSVLLADMRAWLWNEGAMTSRVVEGMEETGAKFRDYFDYREPIA
                     RMPSHRALAAFRGQAEGVLTLGLEVDAERPDQPVERTAAYFNVGTQRRPADAWLWQCC
                     RWTWRVKLRASLEAEVFDRLREAAEGAAIDVFGTNLRDLLLAAPAGHKRVLGLDPGIR
                     TGVKTAAIDETGKLLATAVVFPFGSNERRSDAVATIARLIRQHRLTLVSIGNGTASRE
                     TVEFVEEVKRLHPGLDFTHVVVSEAGASVYSASELAAAEFPDLDVSYRGAVSIARRLQ
                     DPLAELVKIEPKAIGVGQYQHDVDQQKLAHRLDAVVEDCVNAVGVDVNTASSALLCRV
                     AGLSPARANAIVSFRSANGAFRTRRDLLAVPRLGAKTFEQAAGFLRIPDGDDPLDASA
                     VHPEAYPVVLRILKKTGLDIKALIGNAAVLRSLHARDFVDEHFGIPTVTDILGELLKP
                     GRDPRPEFRTAKFADGIKEMKDLQPGMVLEGVVTNVAAFGAFVDVGVHQDGLVHISHL
                     ADRRVSDPRDVVKVGDIVKVRVLEVDLARQRISLSMRSEGARRQAPAARRDTRSAAPS
                     QGAMAAAFAKLRR"
     gene            complement(5492..>5976)
                     /locus_tag="ACZPB6_06510"
     CDS             complement(5492..>5976)
                     /locus_tag="ACZPB6_06510"
                     /inference="COORDINATES: protein motif:HMM:NF020551.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AtaL-like protein"
                     /protein_id="HIR1184152.1"
                     /translation="KTKADSMRKVHEHIIQIPPFVTRDQLWNGLEKAARHPDRFVEHM
                     EGCELLSERLIEGARHLGREVSFGGGFRLRDKVILREGESVTTVVEAGESWPASTFLM
                     KIEEPEAGSLFVRFIYEADEPEEALNPMIEQLRRQAYEAKDRALIEQVLKNIPQGELS
                     "
BASE COUNT         1143 a         1903 c         1832 g         1098 t
ORIGIN      
        1 cctgaaattt ggagtgagcg ctactttttt ttcttggagg gagccgacca gactttttct
       61 taaaagtcaa ggccgcgttc cacggacttc cgagatgaac cctttttttg cccctgcgga
      121 cttgaaaaaa aatcatggtt gaaaccccca aaggccttcg ccttcacatc ggcctcttcg
      181 gcaagcgcaa tgccggcaag agctctctca tcaacgcact gaccaaccag aagatctcta
      241 tcgtctccga agtcgccggc accacgaccg accccgttga aaaggcgtgc gaactcgcgc
      301 cgatcggtcc cgtcgtcttc atcgacacgg cgggcgtcga cgatgtcggc gctctcggac
      361 tcgcccgagt cgaacgcacc agggccgtgc tcgaatgggt cgacatcgcg cttgtcgtcg
      421 ccaacgccag ggacttcgat gagtgtgaga gcgaccttgt cgaacacctg cgcaagctcg
      481 gcacacccgc cgtcctcgtc atcaacaaga gcgacctcca ccctgccgat gaagccttca
      541 tgacgcgcat gcgcgcctac ggcatccccg tcgtgctgac cgacgctgaa acgaaagcag
      601 gcatcgcgga cctccgcaag gccatcatcg acctcgccgc aaaccgcatt cccaaagacc
      661 gcgccctcgt gggcgacctc gttcaggcgg gcgacaccgt cgttctcgtc acgccgatcg
      721 acacaggcgc ccccaagggc cgtctgatcc tgccgcaggt gcaggctatc cgcgagcttc
      781 tcgacgctca tgccaagtgc ctcgtcgtac agcaagaccg cgtggccgaa agcatcgccg
      841 agctccgcca cccgcccaag ctcgtcatgg cggactccca ggtcatcatg gacgtcgccg
      901 cacagacgcc ggaggacatt ccgctcacca ccttctcgat ccagatggcc tactccaagg
      961 ccgacgtcat cgaaatggcc cgcggcaccg ccgcgcttgc ctcgcttgaa gacggagacc
     1021 gcgtgctcat ctgcgaaacc tgcagccatc acccgcagaa ggacgacatc ggccgcatca
     1081 agattccccg ctggcttcgc cagaaaacgg gcaaggacct ccagatcgaa gtggccgtcg
     1141 gcaaggactt ccccgtcgac ctgacgccct acaaggtcct catccagtgc ggtggctgcg
     1201 tcgtcacgcg acgccacatg ctcatgcgtc tgcgcgccgc acatgcacag aacgtgccca
     1261 tgacgaacta cggcctcacc atttcgtacc tgcagggcgt gctcgagcgc gtgctgctct
     1321 gccaccccga agccctcaag gcctacaggg acgcgctcac gaagtaaggc accgcagaaa
     1381 aacaaaacgg catccgaatg cattcggatg ccgttttgtt tttcaagtgc ctatcagact
     1441 cagtcgaacg tctgctgcat ttcgcgcgtg gtgtggaaca ccacctgcgg ccaatgcttc
     1501 atcgtcgtct caaggttgta gatcgacgtg gcaaggtaca ccgtgttgcc gttgacgtcg
     1561 gtcgcaagac gggcgtcctg ctccttgcag aagtccttgc gggtctgctc gtcggggaac
     1621 gagagccagc gggccgtgga gacgtcggtc gactcgtaga tggcgtcaac cttgtactcc
     1681 gtggcaaggc gctgggccac gatttcaaac tgaagctgac cgacggcacc cagaaggatg
     1741 ttgccgaact cgccggtgaa gacctgaatg gcgccttctt cgccgagttc ctgcaggccc
     1801 ttctgaagct gcttggcctt gaacgggtct ttgcttcggg cggcgcggaa gagttccggc
     1861 gcaaagctcg ggatgcccgt gaagccgagc ttctcgcctt cggtaagtgt atcgccgatg
     1921 tggagctggc cgtggttgta gatgccgatg atgtcgcctg caacggcgtc cgtcatcacg
     1981 acgcggtcct gagccatgaa ggtcagggcg ttcgggatct tcatgtcgcg attctcgcgc
     2041 acgtggaaga ccttcatgcc ctgcgaatag cggcccgagc agacacggaa gaacgcaatg
     2101 cggtcgcggt gacgcgggtc catgttggct tgaatcttga agacgaagcc cgagaacggc
     2161 gcctcggtcg gctcgatctt gcggacgccg gcatcacgcg gctgcggcgg cggagcccac
     2221 tgcacgagag cgtcaagaac gtcctgcacg ccgaagttgt tcacaccgga accgaagaag
     2281 acggggctct gctcgcccgc aaggaacttc tcgatgtcga acggttcggt cgcaccctga
     2341 acgagttcga cggactcgcg cacgtacggc atttccatcg ggaagagacg gtcaagctcg
     2401 ggattgtcga gcccttcgat catctcctga tcacgcgaga gacgctcctc gccggccgta
     2461 aagcgcacaa gacggttctt cagcagcgag aagacgccgc ggaaggtctt gcccatgcca
     2521 atgggccagg tgatgggcac gcactggagc ttgagcacgt tctcgatttc gccgatcacg
     2581 tcgagcggat cgcgcacttc gcggtcgagc ttgttgatga acgtgatgat cggcgtcttg
     2641 cgcatgcggc agacttcgag aagcttgatc gtctgggctt ccacgccctt ggcggcatcg
     2701 atcaccatca cggcggcatc gacggcggtc agcactcggt acgtgtcttc agagaagtcc
     2761 tcatggcccg gggtgtcgag caggttgatg acgtgattgt catagtggaa ctgcatcacc
     2821 gaactcgtca cggaaatgcc gcgctgcttt tcgacttcca tccagtcgga cgtggcgtga
     2881 cgggcagcct tgcggccctt gacggcgccg gccatctgaa tggcgccgcc gaacagaagg
     2941 agcttttccg tgagcgtcgt cttgccggcg tcaggatggg aaatgatggc aaaggtgcgg
     3001 cgcatttcga cgtcgctgac caatgcgggg ttgggactcg tcataacaga gaacgttctt
     3061 ttaacgtaaa ttcaatgaaa atcggctctt ccgccgaact gttcgacgga aaagccgatc
     3121 attttaacag agagcagagg atgcttcagc cgaattgcgg ccagaacctc tacctgcgga
     3181 gcttggcaaa cgcagcggcc atggcgccct gcgatggcgc ggcggagcgc gtgtcccgac
     3241 gggctgcggg cgcctgcctg cgcgccccct cgctgcgcat gctgaggctg atgcgctggc
     3301 gtgcaaggtc cacctcgaga accctgacct tgacgatgtc gcccaccttc accacatcac
     3361 gcggatcgga cacgcgccga tcggctagat gagagatgtg cacaagaccg tcctgatgca
     3421 cgccgacgtc cacgaaggcg ccgaaggcgg ccacgttggt caccactccc tcgagcacca
     3481 tgccgggctg caggtccttc atctccttga tgccgtcggc gaacttcgcc gtgcggaatt
     3541 cgggacgggg atcacgaccg ggcttcagaa gttcgccaag aatgtccgtc acggtcggaa
     3601 tgccgaagtg ttcgtccacg aagtcgcggg catggagcga acgaagcacc gccgcattgc
     3661 cgatcagcgc cttgatgtcc agccccgtct tcttcaggat gcgaagcaca acgggatagg
     3721 cctcgggatg cacggcggag gcgtcaagcg gatcgtcgcc gtcgggaatg cgaaggaagc
     3781 ccgccgcctg ctcgaaggtc ttggcgccca gcctgggcac ggccagaaga tcgcgacggg
     3841 tgcggaaggc gccgttggcg ctgcggaagc tgacaatggc gttggcgcgt gcgggcgaaa
     3901 ggccggcaac gcggcacaga agcgcggagc tggccgtatt gacgtcgacg ccgacggcgt
     3961 tgacgcagtc ctcgacgacc gcgtcgaggc gatgcgccag cttctgttgg tcaacgtcgt
     4021 gctgatactg acccacgccg atcgccttgg gctcgatctt gacgagttcc gcgagcggat
     4081 cctgcagacg gcgcgcgatg gagacggcgc cgcggtagct cacgtcaaga tcagggaact
     4141 cggccgcagc gagctcggaa gccgaataga cggacgctcc cgcctcgctc acgaccacgt
     4201 gcgtgaagtc aagccccgga tgaagacgct tgacctcctc gacgaactcg accgtctcgc
     4261 ggcttgccgt gccgttgccg attgagacaa gcgtgagccg gtgctggcgg atgagtcgtg
     4321 caatcgttgc aaccgcatcc gaacgacgct cattggagcc gaacgggaat acaaccgccg
     4381 tcgcaagcag cttgcccgtc tcgtcgatcg cagccgtctt gacgcccgtg cgaatgccgg
     4441 ggtcaagccc aagcacgcgc ttgtggcctg caggcgccgc aagaagcaga tcgcgcagat
     4501 tggtgccgaa gacgtcgatg gccgcgccct cggcagcctc gcgcagcctg tcaaaaacct
     4561 cggcctcgag actggcgcgg agcttgacgc gccaggtcca gcggcagcac tgccagagcc
     4621 aggcgtcggc cggacgcctc tgggttccca cgttgaagta ggcggccgtg cgctccacag
     4681 gttggtcggg acgctctgcg tcaacttcga gacccagcgt gagaacgccc tccgcctgac
     4741 ctcggaaggc ggcgagcgcc ctgtgagacg gcattctggc aatcggctcg cgatagtcga
     4801 agtagtcgcg gaacttggcg cccgtctctt ccatcccttc gacgacgcgg ctcgtcatgg
     4861 cgccctcatt ccaaagccag gcgcgcatgt cggcgagcag cacggagtcc tcgctcatct
     4921 tctcggcaag gatgtcgcgg gcgccgttga gcgcagcctt gacgtcggga acattcttct
     4981 cggggctgac gaaagcgaca gcctcggttt cgggatccag tccggggttc tcgaaaagct
     5041 tgaacgccag cggctcgagc cccgcctcac gcgcaatggc ggcaagcgtg cggcgcttcg
     5101 gacggtaagg gagataaagg tcctcgagtc cctgcttcgt gtccgcggcg acgatcttcg
     5161 cctcaagctc gggcgtgagc ttgccctggt cgcgaatgct gccgagaatc acctcgcgac
     5221 gcttttcaag ctcgcgcaga taaccaaggc gttcctcgag aagtcgaagc tgcgtgtcgt
     5281 cgaggccgcc cgtcacttcc ttgcggtagc gagcgacgaa gggcaccgtg cagccgtcgt
     5341 caagcaggcc gacggccgcc gcaacctgag cgggacgcgc ctgaatgtcg gcggcgatga
     5401 tctcgaaaat tatctgttca gtcgtggtca tggctttcca tgcaaaacaa cggggacgcc
     5461 gcggcgtccc cgggaagcgg atctcacaat tttaggagag ttcgccctgc ggaatgttct
     5521 tgaggacctg ctcgatcagg gccctgtcct tggcctcata ggcctgacgg cgcagctgtt
     5581 cgatcatcgg gttgagcgcc tcttcgggtt catcggcttc atagatgaag cgcacgaaaa
     5641 ggctgcccgc ctcgggctcc tcgatcttca tgagaaaggt ggatgcgggc caggactccc
     5701 ctgcctcgac cacggtcgtc accgactcgc cctccctgag gatcaccttg tcgcgaaggc
     5761 ggaagccgcc gccgaagctc acctcgcggc caagatgcct cgccccctca atgagcctct
     5821 cgctgaggag ctcgcaaccc tccatgtgct cgacgaacct gtcgggatga cgggcagcct
     5881 tctcaagacc gttccagagc tggtcccgcg tcacgaacgg cgggatttgg atgatgtgtt
     5941 catgtacctt gcgcatgctg tctgcctttg tttttt
//
LOCUS       DBMDTI010000118         5964 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3352_length_5964_cov_2.661095, whole genome shotgun sequence.
ACCESSION   DBMDTI010000118 DBMDTI010000000
VERSION     DBMDTI010000118.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3352_length_5964_cov_2.661095"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(246..1643)
                     /locus_tag="ACZPB6_06515"
     CDS             complement(246..1643)
                     /locus_tag="ACZPB6_06515"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HIR1184153.1"
                     /translation="MSRIESDFLGELEIPDEAYYGVQTMRGKHNFNITEMPMSQEPFF
                     AIAYGYVKKASVMTNKELGCIPADVADALIWACDQIIAGKYNDQFVTDWLQGGAGTST
                     NMNCNEVLCNLACEHLGLPKGDKKVSPNDHANFGQSTNDSYPTAFHLSLILRSNVLLK
                     AVEDLVASFYKKAEETKDVLKMGRTHLQDAVPMSFGQEFHGWGFTIADEIKTIKEAQE
                     HLKVVNLGATAIGTTVTTHPEFPAKAVKHLAEITGIDFRNSEDLIAATSDCGSYVALS
                     SAVKSLAVKLTKVCNDLRLLASGPRTGLSELILPQLQPGSSIMPGKVNPVIPEVTNQA
                     SFLAIGLDTTVMLAASAGQLELNVMEPVITFALYMQMKVMTNACNHLREKCIDGIVVN
                     AEHCKDLVMGSLGIITILKPHFGYKPCANIAREGFLTGKSLHQLVVEEKHMLTQEQWD
                     KVFTYENLINPKFEK"
     gene            complement(1831..2646)
                     /locus_tag="ACZPB6_06520"
     CDS             complement(1831..2646)
                     /locus_tag="ACZPB6_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HIR1184154.1"
                     /translation="MADIQQTTNKQHRMLKISILRFNPQDPESVPHMQTYELEESDSM
                     TLFIALNELRDTQDPTLQFDFVCRAGICGSCAMMINGKPGLACRTLTRDLPTEFTLAP
                     LPAFELIGDLSVNTGKWMRNMSERMETWVHMKEEEVDLRKKEVPMDPQLAEDIYVLDR
                     CVECGCCIAGCGTVRIRPDFVGGVGINKIARFRLDPRDTRNDADYYDIVGDESGVFGC
                     MSLLACHDFCPKELPLKTQIAFIRRKMAEQGMRGYDKVLPTQKKAIPIKPARQ"
     gene            complement(2667..4652)
                     /locus_tag="ACZPB6_06525"
     CDS             complement(2667..4652)
                     /locus_tag="ACZPB6_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase flavoprotein subunit"
                     /protein_id="HIR1184155.1"
                     /translation="MKIIYTDVLVIGGGLAGLRMAVAAKRRGHDSIVLSLVPPKRSHS
                     KAAQGGMQASLGNVIKGVGDNEDVHFGDTVRGSDWGADQEVVRMFVNTSPKAVRELAA
                     WGVPWSRITPGDRQVIINGEKVTITERKEAAGLVAQRDFGGTKKWRTCYVSDCTGHSM
                     LNAVSDRIIAEQVPVIERVEALSLIHDGKRCYGAIVRCLITGELMAYVSKATAIATGG
                     AGRIYRVTTNAVICEGTGYDLALETGVASLGNMEAIQFHPTGIFPAGILVTEGCRGDG
                     GLLRDVDGHRFMPDVEPEKKELASRDVVSRRMEERIAQGKGVKSRFGEHIWLDITLLG
                     EHHIKHKLREVYEICHYFLGVDPTKEWVPVRPAQHYTMGGVRTKPTGESPTLKGLFAA
                     GEAACWDMHGFNRLGGNSVAETVVAGMIVGEFIADFCDKAENGIDIPTSLIYGALAKE
                     EARLKSFMNNGGSENAYEIRTRMQEIMTTKIGIFRKGADMESAVAELEELYKRSFKVS
                     VKDVVGPNPELIYAYRTQKMLRVALSVAYGALNRKESRGAHYREDFPVRNDIEWLNRT
                     IATWKEGDTLPTLNYEKLDISKMELPPGFRGYGVKNYIENPESAKRQAEVDAIRAKME
                     AAGADRFAIQDALMPYQDLLPKRLKGKNERVDEPLKD"
     gene            5490..5870
                     /locus_tag="ACZPB6_06530"
     CDS             5490..5870
                     /locus_tag="ACZPB6_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HIR1184156.1"
                     /translation="MPVNPVHTDNAPAAIGAYSQATLAEGFLFTSGQLGLVPATGALA
                     EGVEAQARQAFANLEAICAAAGTTLGNTVKMTIFLADINEFAVVNKVMTEVMSEPYPA
                     RSCFAVAALPKAARVEIEAVVTMP"
BASE COUNT         1165 a         1660 c         1823 g         1316 t
ORIGIN      
        1 ccaatgccga tgccgccaag gcggacaccg aggaagatcg cggcgagaac gaccacgaac
       61 tggagaaggg tgatgaagtc catattagct tcaaaaacgg tgggagccgc ttttgaatcc
      121 tctcttttct ttggttggtt aatcgaagca atccggcttg ggcaggagcc caaaccggag
      181 tgcttcgttt ttttttgtcc gggaccaccc tttttcagag cggcctggat ccagacttta
      241 ttatcttact tttcgaactt cgggttgatg aggttttcgt acgtgaagac cttgtcccac
      301 tgttcctggg taagcatatg cttttcttcg acaacgagct ggtggaggga cttgccggtg
      361 aggaagcctt cacgagcgat gttggcgcag ggcttgtagc cgaagtgcgg cttcaggatc
      421 gtgatgatgc cgagggagcc catgacgaga tccttgcagt gttcggcgtt cacgacgatg
      481 ccgtcgatgc acttttcacg cagatggttg caggcattcg tcatgacctt catctgcatg
      541 tagagggcga acgtgatgac cggttccatg acgttcagtt cgagctggcc ggcggaagcg
      601 gcgagcatga ccgtcgtgtc gagaccgatg gcgaggaagg aagcctggtt ggtgacttcc
      661 gggatcacgg ggttgacctt gcccggcatg atggaggaac ccggctggag ctgcggaagg
      721 atgagctcgg agagacccgt acgcggaccg gaagcgagga gacggaggtc gttgcagacc
      781 ttcgtgagct tgacggcgag gctcttcaca gcggaggaga gagcaacgta ggagccgcag
      841 tcggacgtag cagcgatgag gtcttcgctg ttgcggaagt cgatgccggt gatttcagcg
      901 aggtgcttga cagccttggc cgggaattcc ggatgcgtcg tcacggtcgt gccgatggcg
      961 gtagcgccga ggttgacaac cttgaggtgt tcctgagctt ccttgatcgt cttgatttcg
     1021 tcggcgatgg tgaagcccca gccgtggaat tcctgaccga aggacatcgg aacggcgtcc
     1081 tgaaggtggg tacggcccat cttgagaacg tccttggttt cttcagcctt cttgtagaag
     1141 gaagcgacga ggtcttcaac agccttgaga aggacgttgg agcggaggat cagggacagg
     1201 tggaaggccg tcgggtagga gtcgttcgtg gactgaccga agttggcgtg gtcgttcggg
     1261 gagaccttct tgtcgccctt cgggaggccg aggtgttcgc aagcgaggtt gcagagcact
     1321 tcgttgcagt tcatgttcgt ggacgtgcca gcgccaccct ggagccaatc ggtgacgaac
     1381 tggtcgttgt acttgccagc gatgatctga tcgcaggccc agatcagggc gtcagcgacg
     1441 tcggccggga tgcagccgag ttccttgttc gtcatcacgg aggccttctt cacatagccg
     1501 taggcgatgg cgaagaacgg ttcctgggac atcggcattt cggtgatgtt gaagttgtgc
     1561 ttaccacgca tcgtctggac gccgtagtag gcttcatccg gaatctcgag ttcgccaagg
     1621 aagtcgcttt caatacgaga cattttattt gctcctcaaa tattcgggtc ggaccagaca
     1681 gggagaattc tctgccggtg atcctcaccg tttgtgtttc ccgcggggcg ttatgtttgg
     1741 tcctgaacgc tggtcccggg aaacgggtcg aaaccgcaag aggtgatcaa atgatctcaa
     1801 atcaaaagat cacctctcac gacctacatt ttactgccta gccggcttaa tgggaatagc
     1861 ctttttctgg gtcggcagga ccttgtcgta gccacgcatg ccctgttcag ccatcttgcg
     1921 acggatgaag gcgatctgcg tcttcagggg aagttccttc gggcagaagt catggcaggc
     1981 aaggagggac atgcagccga acacgccgga ttcgtcaccg acgatgtcgt agtagtcggc
     2041 gtcgttacgc gtgtcgcgcg ggtcgagacg gaagcgggcg atcttgttga tgccaacgcc
     2101 gccgacgaag tccgggcgga tgcgaaccgt accgcaaccg gcgatacagc agccgcattc
     2161 cacgcagcgg tcgagaacgt agatgtcttc ggcaagctgc ggatccatcg ggacttcctt
     2221 cttgcgaagg tcgacttcct cttccttcat gtgaacccac gtttccatgc gttcggacat
     2281 gttgcgcatc cacttgccgg tgttgaccga gaggtcgccg ataagttcga aggcgggaag
     2341 cggggcgagc gtgaattccg tcggaaggtc acgcgtcagc gtacggcagg cgagacccgg
     2401 cttgccgttg atcatcatgg cgcaggaacc gcagatgccg gcgcggcaga cgaagtcgaa
     2461 ctggagcgtc ggatcctggg tgtcgcgaag ttcattgagc gcaatgaaca gcgtcatcga
     2521 gtcggattct tccagctcat aggtctgcat gtgcggcacg ctctcaggat cctgcgggtt
     2581 gaagcgcagg atgctgatct tgagcatgcg gtgctgcttg ttggtggtct gttgaatatc
     2641 agccattttg acgtcaatct ccaaaattag tctttcagcg gttcatcaac gcgttcgttc
     2701 ttgcccttga ggcgcttcgg cagcagatcc tgatagggca tgagggcgtc ctgaatagcg
     2761 aagcggtcgg caccggcggc ttccatcttg gcgcggatgg cgtcaacttc agcctggcgc
     2821 ttggccgatt ccggattttc gatgtagttc ttcacaccgt agccgcggaa gcccggggga
     2881 agttccatct tgctgatatc aagcttttcg tagttgaggg tcggcagcgt gtcgccttcc
     2941 ttccacgtag cgatcgtacg gttgagccat tcgatgtcgt tacgaaccgg gaagtcttcg
     3001 cggtagtgag cgccgcggga ttccttgcgg ttgagagcgc cgtaggccac ggagagggcc
     3061 acgcggagca tcttctgggt acggtaggcg tagatgagct cggggttcgg gccgaccacg
     3121 tccttgacgg acaccttgaa ggagcgcttg tagagctctt caagttcagc gacggcggat
     3181 tccatgtcag cgcccttgcg gaagatgccg atcttcgtcg tcatgatttc ctgcatgcgg
     3241 gtgcggattt cataggcgtt ttcagagccg ccattgttca tgaaggactt caggcgagcc
     3301 tcttccttgg cgagagcgcc gtagatgagg gacgtcggga tgtcgatgcc gttttcagcc
     3361 ttgtcgcaga agtcggcgat gaattcaccg acgatcatgc cggccacgac ggtttcagca
     3421 acggagttgc cgccgaggcg gttgaagccg tgcatgtccc agcaggcggc ttcgccggca
     3481 gcgaagaggc ccttgagcgt cggggattcg ccggtcggct tcgtgcgcac gccacccatg
     3541 gtgtagtgct gggccggacg gacgggcacc cattccttcg tcgggtcaac gccgaggaag
     3601 taatggcaga tttcgtagac ttcacgcagc ttgtgcttga tgtggtgttc gccaagaagc
     3661 gtgatgtcga gccagatgtg ttcgccaaag cggctcttca cacccttgcc ctgggcaatg
     3721 cgttcttcca tgcggcggga aaccacgtcg cgggaggcga gttccttctt ttccggttcg
     3781 acgtccggca tgaagcggtg gccgtcgacg tcgcggagca gaccgccgtc accacggcaa
     3841 ccttccgtga cgagaatgcc ggcggggaag atgccggtcg ggtggaactg gatggcttcc
     3901 atgttgccga gggaggcgac gcccgtttcg agagcgaggt cgtagccggt gccttcgcag
     3961 atgacggcgt tcgtcgtcac gcgatagata cggccggcac cgcccgtggc gatggccgta
     4021 gccttggaca cgtaggccat gagttcgccc gtgatgaggc agcgaacgat ggcgccgtag
     4081 caacgcttgc cgtcgtgaat cagagagagg gcttcaacgc gttcgatgac cgggacctgt
     4141 tcggcgatga tgcggtcgga aacggcgttg agcatggagt gaccggtaca gtcggacacg
     4201 tagcaggtac gccacttctt cgtgccgccg aagtcacgct gggcgacgag gccggcagct
     4261 tccttgcgtt cggtaatggt gaccttttca ccattgatga tgacctggcg atcacccggg
     4321 gtgatgcggc tccagggcac accccaggca gcgagctcac gcacggcctt cggggaggtg
     4381 ttcacgaaca tgcggacgac ttcctggtca gcaccccagt cggagccgcg gaccgtgtcg
     4441 ccgaagtgga cgtcttcgtt gtcgccgacg cccttgatga cgttgccgag cgaagcctgc
     4501 atgccgccct gagcagcctt ggaatggctg cgcttcggcg gaaccagcga caggacgatc
     4561 gagtcatgcc cacggcgctt ggcagcaacg gccatacgca gaccggcaag accgccaccg
     4621 atcacgagca cgtcggtgta gataattttc atttccaatg gtctcctaga tccaaagacg
     4681 atgtgccggg agcgaaactg gggttccgcc ctgcccggca cggggtcaga ttccgtcaat
     4741 tcacctgaat cgccgttctc ctggtgcgac tcagttgaac tggcctcttc acgaccttgc
     4801 ggcagtcgtt caagaggcgg actttgaagc aagaaagtaa cgctttcttt ctccgcattc
     4861 cttatatcag ctttgccgaa atcgggtagc gggaaagtcg ggaggatgac gtctgcgtca
     4921 aactttgccc aatctttacg atctgccttc tcgaaaagct acgtatttct acggtcaccc
     4981 gtgtcagcat cgattctttc gaactaggca aaacacccaa ggcggctttt cttaagtcac
     5041 gcgcagttta ttactactac cacctcagaa acattcgggg taaccctagg tttctgtttt
     5101 ttttcttacg attcagtcaa gggtcctcaa atctctgatc gccccgcttc tcctgattcc
     5161 ccaagcattc gcccgatccg ccctccggcg ccccccgaac gggctcctac ctaggactgc
     5221 gcgccaacct atcccaaggt actaccactc aagtaataca cgttaacccc taaaatacct
     5281 taaatgttaa tctcgttgta ggccgtctcg cctagggaat tctgcgtatt tatactcact
     5341 tttcctcctc ataggcttta tagtgttcct taacaaatcc gatagaatca ccccctatcg
     5401 tgcaaaattt tccgatagaa tataggcatt cagcggattt cgccgcaacg cgcccgacat
     5461 gcgcgcaccg acttcggagg atacccacca tgcccgtcaa ccccgtccac accgacaacg
     5521 cccccgccgc cattggcgcc tacagtcagg cgacgctcgc cgagggcttc ctcttcacgt
     5581 cgggccagct tggtctagtg cccgcaacgg gcgcacttgc cgagggcgtc gaggctcagg
     5641 ctcgacaggc cttcgccaat ctcgaggcga tctgtgccgc agcgggcacc acgctcggca
     5701 acaccgtcaa gatgacgatc ttccttgcag acatcaacga gttcgccgtg gtcaacaagg
     5761 tgatgaccga agtcatgtcc gagccctatc ccgcccgctc ctgcttcgcg gtcgccgcac
     5821 ttcccaaggc cgcccgcgtc gagatcgaag ccgtggtgac gatgccgtaa ggcaatcccc
     5881 catctttgta tatatgcaga acatggccgc cgccccgaaa cagcaggagc gcgcctcggg
     5941 cgtcatccgt caggagaagc tcgc
//
LOCUS       DBMDTI010000119         5763 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3469_length_5763_cov_2.871953, whole genome shotgun sequence.
ACCESSION   DBMDTI010000119 DBMDTI010000000
VERSION     DBMDTI010000119.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3469_length_5763_cov_2.871953"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            198..1538
                     /locus_tag="ACZPB6_06535"
     CDS             198..1538
                     /locus_tag="ACZPB6_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1184157.1"
                     /translation="MSGEQRKTWTSRIGFVLASAGAAVGLGAIWKFPYMAGTNGGSAF
                     LFPYILMTFTVGAALLIAEVALGRAGRGGIVTAYRNLAGRAWVPAGYLGVLTGFLVLC
                     FYSAIGGWTLAYFAEAATGSGLITDQAELGKHFGSFVSDPVQALGFQWLFLLLNGLIV
                     ALDVTKGIERISKFLMPLLFVMMLVLIVRGLMLPGAIGGLEFLFKFDPDAFTWSALLQ
                     AMGFTFFSLCVGCGCMLTYGSYLDQNSNLVNGCLWITFLAVIASILGGLMIMPSVFAF
                     GLDPSAGPGLTFITMPAVFAQLPFGQVFAVIFYLCIAVAAITSSVSMIEIVVAFLVDE
                     HKMKRAGAAMLSTLALAVVGALPCLSFGKLSDVTFFGGKTIFDLFDFFTSNISLPVGG
                     LIILWLAGVRCWPQIRENITRNTSLSEGAVKFLRLMMIGFSPVLVLVVLVSGLV"
     gene            1632..2969
                     /locus_tag="ACZPB6_06540"
     CDS             1632..2969
                     /locus_tag="ACZPB6_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1184158.1"
                     /translation="MTERSVWGSRLGFILASAGSAVGLGAIWKFPYMAGTNGGSIFML
                     PYIFFTVTIGLTLLIGEMCMGRSVRAGAAGAFRRLGGPFTGIIGYISVLTGFLVLAFY
                     SVVGGWCVYYMIQAFMGNGIINDPSMMRGAFESFASSDVAPVLSHLTFLGATAFVVYF
                     GVEKGIERVSKVLMPLLFFMMLILIVRGLMLPGAMKGVEFLFKPDLSAFTGESLLNAM
                     GFAFFSLSVGSGSQMNYGSYLSDRVHLPSSVSWIVFLAILSAILGGLMIMPAVFAFGL
                     SPDAGPGLTFATMPAVFAQLPFGHFFAITFYVCLFVAALTSSISILEMSVQFLHDEFN
                     ISRPAGVTITGSILAVLGTLCALSFGTLSHVTIFGKSFFDFFDYLTSNIGMPIGVIGI
                     AIVTAWLAWPTTVNQLSLVKPVSGFFLKSYRVMAGIFAPILILVVMLKGLGVF"
     gene            3060..4397
                     /locus_tag="ACZPB6_06545"
     CDS             3060..4397
                     /locus_tag="ACZPB6_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1184159.1"
                     /translation="MSENQTTTSWGSRLGFILASAGSAVGLGAIWKFPYMAGTNGGSV
                     FMLPYIFFTFTVGISLLVAEFAMGRAGREGPMASLTKVCGRGWGFFGGVGVFTVFLIL
                     SFYSGVGGWCIKYMVDAVIGRGLISDPELLGTHFGAFVSDGSMSFLYMMIFLGITSVV
                     VLRGIQGGIERVAKVLMPALFILMLIIIVRGLTLPGGLAGLEYLFAPRWEDFNASALF
                     NAMGFCFFSLSLGAGTMITYGSYLSPKADLPGAVGWVAVLAIMSSILGGLMVLPAVFA
                     FGLDPSAGPGLTFVTMPAVFSKMPLGQLFAVMFYVCLVVAALTSSVSMLEACTALLTR
                     QFNMRRTPAILLTVASCTVVGLASTLSFGVWSDVKFFNRTIFDILDYVTSNVGMPLST
                     FGIAIAAAWVAWPTMKRELTAARPVSESGLWFIRIMIGVVSPLFVLVVAGGSL"
     gene            4397..4753
                     /locus_tag="ACZPB6_06550"
     CDS             4397..4753
                     /locus_tag="ACZPB6_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184160.1"
                     /translation="MTEDSARRLVYAVLAQEYRAHRSLWIGTLCLALGAAMISGLVVD
                     GASSLRNAASIVIVLGALYLMVLLVLRQELSARSAGLPPDEAIAFRKGLTSGLLRACA
                     PAVLAIIVCSTALLVL"
     gene            4825..5613
                     /locus_tag="ACZPB6_06555"
     CDS             4825..5613
                     /locus_tag="ACZPB6_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024634.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1184161.1"
                     /translation="MSPNRIIVARFSPTGRTRRLMSMIAQGLAGTALPVEELDLTDRW
                     EREEARTFGKGDLVFLGVPVYFGRVPLPMQSLAKWTGNGAAAVPVAAYGCRAHEDTLR
                     ELAAVLRAAGFVVPAGAAFPVEHSQFPEFGAGRPNEADRFSAGDFARRVFEALRDGTA
                     RFAPEFPGEGELRPYPKTGFVPMPDPVCRMCGRCAEVCPMDIIDPFSMRVKDPSQCIG
                     CRACIDACPDSHRNFPESVRATMAQVRERIRPACEAPKFPEFFF"
     gene            5631..>5763
                     /locus_tag="ACZPB6_06560"
     CDS             5631..>5763
                     /locus_tag="ACZPB6_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014047.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HIR1184162.1"
                     /translation="MTTRHLTITDIASTGAGIAYEDGLRVFVPGALPGDEVTAEVDPP
                     "
BASE COUNT          824 a         2148 c         1588 g         1203 t
ORIGIN      
        1 cgattcttac agtcaggcaa agtcctcacg cgcgtcgtcc gccaaaaaaa gcagcagaag
       61 cctcatgagc aaactgcaac cgggacaccg cttccgaagg acctgtcgca taaaagagtc
      121 aatcaggcgg catcaagacc gcctctgcct tccgcgcgcc tcccgatggg gcggactgcg
      181 gatcaaggag tgtacttatg tccggagaac agcgaaagac ctggacgagc cgaatcggct
      241 tcgtgctcgc cagcgccggt gctgccgtgg gtctgggggc catctggaag ttcccctaca
      301 tggcgggcac gaacggcggc agcgcctttc tcttccccta catcctcatg accttcaccg
      361 tgggcgccgc cctgctgatc gccgaagtgg cgctcggccg tgcgggccgc ggcggcatcg
      421 tgacggccta ccgcaacctc gcgggccgcg cctgggtgcc cgcaggctat ctcggcgttc
      481 tcacgggctt cctcgtcctg tgcttctatt cggccatcgg agggtggacg ctcgcctact
      541 tcgccgaagc ggccacgggc tcgggcctca tcaccgacca ggccgagctc ggcaagcact
      601 tcggcagctt cgtctccgat cccgtccagg cactcggctt ccagtggctc ttcctgctgc
      661 tcaacggcct catcgtggcg ctcgacgtca cgaagggcat cgagcgcatc tccaagttcc
      721 tgatgccgct cctcttcgtg atgatgctcg tgctcatcgt gcgcggcctg atgctccccg
      781 gcgccatagg cggccttgag ttcctcttca agttcgatcc cgacgccttc acctggagcg
      841 ccctgctgca ggccatgggc ttcactttct tctcgctgtg cgtgggttgc gggtgcatgc
      901 tcacctacgg ctcgtacctt gatcagaact cgaacctcgt caacggctgc ctctggatca
      961 cgttccttgc ggtcatcgcc tccattctcg gcggcctgat gatcatgccg agcgtcttcg
     1021 ccttcggact cgacccgtcc gcaggtcccg gcctcacctt catcacgatg cccgccgtct
     1081 tcgcgcagct tccgttcggt caggtcttcg ccgtgatctt ctacctgtgc atcgcggtgg
     1141 cggccatcac gtcgtccgtc tcgatgatcg agatcgtcgt cgccttcctc gtcgacgagc
     1201 acaagatgaa gcgtgcgggc gcggccatgc tctccacgct cgcgctcgcc gtcgtcggcg
     1261 ccctgccctg cctctcgttc ggcaagctct ccgacgtcac cttcttcggc ggcaagacga
     1321 tcttcgacct cttcgacttc ttcacgagca acatctccct gcccgtgggc ggcctgatca
     1381 tcctgtggct cgcaggcgtc agatgctggc cgcagatccg cgagaacatc acgcgaaaca
     1441 cctctctctc cgagggcgcc gtgaagttcc tccgcctcat gatgatcggc ttctctccgg
     1501 tcctcgtgct tgtggtgctc gtctcgggcc ttgtctgacg gcccgcggcg acaccgcacc
     1561 caaacacaac ccgcggcggt ccggctccag aaagccgaac cgccttgacc attagaaata
     1621 caagcaaaaa aatgacagaa cgttcagtat gggggtcccg cctggggttt attctcgcga
     1681 gcgcaggctc cgccgtcggt ctcggtgcca tttggaagtt tccctacatg gctggcacga
     1741 acggcggctc catcttcatg ctcccctaca tcttcttcac cgtgacgatc ggcctcacgc
     1801 ttctcatcgg cgagatgtgc atgggccgct cggtacgcgc aggcgccgca ggcgccttcc
     1861 gaaggctcgg cggccccttc acgggcatca tcggatacat ctccgtgctc acgggcttcc
     1921 tcgtgctcgc cttctactcc gtggtgggcg gctggtgcgt ctactacatg attcaggcct
     1981 tcatgggcaa cggcatcatc aacgatccgt ccatgatgcg cggcgccttc gagtccttcg
     2041 cctcctccga cgtcgccccc gtgctctcgc acctcacctt cctcggcgcc accgccttcg
     2101 tcgtctactt cggcgttgaa aagggcatcg agcgcgtctc caaggtcctg atgccgctcc
     2161 tcttcttcat gatgctcatc ctcatcgtgc gcggcctcat gctccccggc gccatgaagg
     2221 gagtcgaatt cctcttcaag cccgacctct ccgccttcac gggcgagtcg ctgctcaacg
     2281 cgatgggctt cgccttcttc tcgctctccg tgggctcggg ctcccagatg aactacggca
     2341 gctacctcag cgaccgcgtt caccttccgt cgtccgtctc ctggatcgtc ttcctcgcga
     2401 ttctctcggc cattctcggc ggtctgatga tcatgcccgc cgtcttcgcc ttcggcctct
     2461 cgcccgacgc tggccccggc ctcaccttcg cgacgatgcc cgccgtcttc gcgcagctcc
     2521 cgttcggcca cttcttcgcg atcaccttct acgtgtgcct cttcgttgcg gccctcacgt
     2581 cctcgatctc gattctggag atgtccgtgc agttcctcca cgacgagttc aacatcagcc
     2641 gccccgccgg cgtcacgatc acgggctcga tcctcgccgt tctcggcacg ctctgcgcgc
     2701 tcagcttcgg cacgctctcg cacgtgacga tcttcggcaa gagcttcttc gacttcttcg
     2761 actacctcac gtccaacatc ggcatgccga tcggcgtgat cggcatcgcc atcgtcacgg
     2821 cctggctcgc ctggcccacg accgtgaacc agctctcgct cgtcaagccc gtctccggct
     2881 tcttcctcaa gagctaccgc gtgatggccg gcatcttcgc accgatcctc atcctcgtcg
     2941 tgatgctcaa gggcctcggc gtcttctgag tccatttgac ctcattccaa taaattcccc
     3001 gccgtcaggc cacccgatac gaacggcggg caaaaagcac aagatcggag tccaacccaa
     3061 tgtctgaaaa ccaaaccacc acaagctggg gctcccgcct cggcttcatt cttgcaagcg
     3121 caggctcggc cgtcggcctc ggcgccatct ggaagttccc gtacatggcg ggcacgaacg
     3181 gcggctccgt cttcatgctg ccctacatct tcttcacctt caccgtcggc atctcgctgc
     3241 tcgtggccga attcgccatg ggccgcgccg gacgcgaggg cccgatggcc tcgctcacca
     3301 aggtctgcgg caggggctgg ggcttcttcg gcggcgtcgg cgtcttcacg gtcttcctga
     3361 tcctctcgtt ttactcgggc gtgggcggct ggtgcatcaa gtacatggtc gacgccgtca
     3421 tcggccgcgg cctcatcagc gatcccgaac tcctcggcac gcacttcggc gccttcgtct
     3481 ccgacggttc gatgagcttc ctctacatga tgatcttcct cggcatcacg tccgtcgtcg
     3541 tgctccgcgg cattcagggc ggcatcgaac gcgtcgccaa ggtgctcatg cccgcgctct
     3601 tcatcctgat gctcatcatc atcgtgcgcg gcctcacgct gcctggcggc ctcgccggcc
     3661 tcgagtacct cttcgcgccc cgctgggagg acttcaacgc ctccgcgctc ttcaacgcga
     3721 tgggcttctg cttcttctcg ctctcgctcg gcgccggcac gatgatcacc tacggctcct
     3781 acctgagtcc caaggccgac cttccgggcg cagtgggatg ggtcgccgtc ctcgccatca
     3841 tgtcctcgat tctcggcggt ctaatggtgc tccccgccgt cttcgccttc ggtcttgacc
     3901 cgagcgccgg ccccggcctc accttcgtga cgatgcccgc cgtcttctcg aagatgccgc
     3961 tcggccagct cttcgccgtc atgttctacg tctgtctcgt cgttgcggcc ctcacctcgt
     4021 ccgtctcgat gctcgaagcc tgtacggcgc tcctcacccg ccagttcaac atgcgccgca
     4081 cgccggccat cctgctcacc gtcgcgagct gcacggtcgt gggccttgcc tccacactct
     4141 ccttcggcgt ctggagcgac gtcaagttct tcaacaggac gatcttcgac atcctcgact
     4201 acgtcacgtc caacgtcggc atgccgctct caaccttcgg catcgccatc gctgccgcct
     4261 gggtggcatg gcccacgatg aagcgcgagc tcacggccgc ccgccccgtc tccgagtcag
     4321 gcctctggtt catccgcatc atgatcggcg tcgtcagccc gctcttcgtg cttgtcgtcg
     4381 ccggcggcag cctctgatga ccgaggattc cgcacgccgc ctcgtgtacg cggtgctcgc
     4441 acaggagtac cgcgcacaca ggtcgctctg gatcggcacc ctgtgcctcg cgctcggcgc
     4501 cgccatgatt tcgggcctcg tcgtcgacgg ggcctcctca cttcgaaacg ccgcgagcat
     4561 cgtcatcgtc ctcggcgccc tctatctcat ggtcctcctc gtactccggc aggagctttc
     4621 agcccgttcg gcaggtctgc cgcccgacga ggccatcgcc ttccgcaaag gcctcacctc
     4681 gggacttctc cgtgcgtgcg cacccgccgt cttggctatc attgtgtgca gtacggcgct
     4741 tctcgtcctc tgatccttgg caccacggga cggacgccgg ctctcgcccc ctgtgcggga
     4801 gcctcacgca gaggaatcag aaccatgtcc cccaaccgca tcatcgtcgc ccgtttctcc
     4861 ccgacgggcc gcacccgccg ccttatgagc atgatcgccc agggccttgc aggcaccgcc
     4921 cttcccgtcg aggagctcga cctcaccgac cgctgggaac gcgaggaggc ccgcaccttc
     4981 ggcaaaggcg atctcgtctt tctgggcgta cccgtctact tcggccgcgt gccccttccg
     5041 atgcagtccc tcgccaagtg gacgggcaac ggtgcggctg cagtgcccgt cgccgcctac
     5101 ggctgccgcg cgcatgagga cacgctccgc gagctcgcag ccgtgcttcg cgccgcgggc
     5161 ttcgtcgtgc ccgcaggcgc ggccttcccc gtcgagcaca gccagttccc cgagttcggt
     5221 gcaggccgtc ccaacgaagc cgaccgcttc tcagcgggcg acttcgcacg cagggtgttc
     5281 gaggcgctca gggacggcac ggcgaggttc gcccccgaat ttccgggcga aggggagctc
     5341 cgtccctatc cgaagaccgg cttcgtgccc atgcccgacc ctgtctgccg catgtgcggc
     5401 cgctgtgccg aggtctgccc gatggacatc atcgatccct tcagcatgcg cgtgaaggat
     5461 ccctcgcagt gcatcggctg ccgcgcctgc atcgacgcct gccccgacag ccaccgcaac
     5521 ttccccgagt ccgtccgagc caccatggcc caggtgcgcg agcgcatccg ccccgcctgc
     5581 gaggctccga agttccccga attcttcttc tgacaacgca ttctcctccg atgacgacca
     5641 ggcatctcac catcaccgac atcgcctcga cgggcgcagg catcgcctac gaggacggac
     5701 tccgcgtctt cgtgccgggg gcgctccccg gcgacgaggt caccgcggag gtcgatccgc
     5761 ccg
//
LOCUS       DBMDTI010000120         5749 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3474_length_5749_cov_3.089943, whole genome shotgun sequence.
ACCESSION   DBMDTI010000120 DBMDTI010000000
VERSION     DBMDTI010000120.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3474_length_5749_cov_3.089943"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="ACZPB6_06565"
     CDS             complement(<1..111)
                     /locus_tag="ACZPB6_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013530.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1184163.1"
                     /translation="MKNILVIGAAGQIGSELVPELRRRGYMTVAAGSGRTP"
     gene            complement(254..730)
                     /locus_tag="ACZPB6_06570"
     CDS             complement(254..730)
                     /locus_tag="ACZPB6_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HIR1184164.1"
                     /translation="MSGHESANPMVTDVFVKGAVQGWNIAVTSTIPNVLMAFVIIKVL
                     NHSGLLTIFGELFAPIMAIFGLPGEAAAVLLGGWMSMGGGVGVAVSLFDQGLLTGEHV
                     AIVAPSIYLMGSQVQYMGRCLGVIGIKGSNLFKIMALPIVTAFVSLFVMKLIVLAS"
     gene            complement(764..1462)
                     /locus_tag="ACZPB6_06575"
     CDS             complement(764..1462)
                     /locus_tag="ACZPB6_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR1184165.1"
                     /translation="MTPEKTTEQAVNEKVGIGAYLSLAFAVVFFSGLCASSQWWGVFD
                     FTTVNGGFGRLVSGVTEQGDAIKTAMANFRGKGGSGAIDGFMFALSLVPTVMFALAMI
                     TVFEHYGALKAARKILTPVLRPLIGIPGSAALALIASMQSTDGGAALTRQLKDAGELT
                     ESETNTFAAFQMTADAPITNFFSSGAVLFTLVAADGTLAVPVSLGLCLGVILLGKVLS
                     ANMMRLLQIRASRK"
     gene            1902..3482
                     /gene="hflK"
                     /locus_tag="ACZPB6_06580"
     CDS             1902..3482
                     /gene="hflK"
                     /locus_tag="ACZPB6_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR1184166.1"
                     /translation="MPLNDPRWGRSSDEEPRRDDRSADEEPPRRPDEDDDRRDDNRRD
                     DRRRGDRRDDNDGMDDLDRLWKEFSELLHGATGRDRKEDRRHPDLRSRDDFSRDDVDE
                     RDDRYGRDEDRDDERVERPQARRYGDDDRRDDRADRPTGARGIFGGLGGMGGLGGFGG
                     KRPNLPAGRKGAGIGMSVIVGLAVLAWGATGFYIVPEGQSGIVTTFGRYAETTAAGFR
                     WHLPMPIQDVQIVDVSSVRTTEIGFAGRSDRLREALMLTDDENIVDVQFTVQYRIKPG
                     DGARDYVFRIRQPDLTVTQAAESAMREVVGRKAMDSVLFESKAEIAEAVKKSMQDMLD
                     RYSSGIEVMSVAIQNAQPPQQVQAAFNDAVKAGQDRERSINLGEAYRNAVIPKAEGTA
                     ARLKEEALGYQARIVETARGDADRFSALQSQYARAPEVTRDRLYVDTVRDVMKNVTKV
                     YVDAKGGNNLLYLPLDKIVNNTKSDAMTQAQKEAAALADHGVTAAQSAVETTGSAQPT
                     TSSAAPGDEYQRMLRDRR"
     gene            3495..4367
                     /gene="hflC"
                     /locus_tag="ACZPB6_06585"
     CDS             3495..4367
                     /gene="hflC"
                     /locus_tag="ACZPB6_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR1184167.1"
                     /translation="MKKLSTLLIAVLVAAGLLRMCVYTVNEREYALVFALGELKTVIS
                     EPGLHVKLPPPLQNIVYLDKRILNLDAAGADLVQTSEKKNLLIDTFVKWRIEDPRLYW
                     VSFQGSEPAAANRVSALLRDVLNVVVNKRTVNQITSSERDKAMVEISQLLQERVRDVG
                     IGIVDVRMKRVDFTPEISESVYRRMEAERKRVASEERSKGAAQAERIRASADRESEVI
                     LAQAYRDAQKVKGEGDAQAAEIYAEAFGRDPEFARFYRSLQAYRDSFADKNDVMVVDP
                     SADFFRYFKSEKAQ"
     gene            4364..4552
                     /locus_tag="ACZPB6_06590"
     CDS             4364..4552
                     /locus_tag="ACZPB6_06590"
                     /inference="COORDINATES: protein motif:HMM:NF021366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HIR1184168.1"
                     /translation="MSRLDVMMLALGWVLVIEGFTPMVAPGFWKESISRFLDEPDRAL
                     RITGLLLVALGLAIIWIV"
     gene            4941..>5749
                     /locus_tag="ACZPB6_06595"
     CDS             4941..>5749
                     /locus_tag="ACZPB6_06595"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR1184169.1"
                     /translation="MAKNVVVVGAQWGDEGKGKIVDWLTEKVAGVVRFNGGHNAGHTL
                     VINGKKTILRLIPSGIMHATSVCYLGNGVVLSPEAFFREVDELNAIGVPNVEKRLRVS
                     ANAALIMPYHVALDHAREAAAGDKKIGTTGRGIGPAYEDKIARRTLRVADLFDKERFV
                     EQVRSVLKYHNFVLENYLGAKPLDADEVIDHMLSYAERLRPMVCDVSAELHQQCAEGK
                     QFLFEGAQGSMLDIDHGTYPYVTSSNTVAGSACAGAGVGPHMLNYVLGITKA"
BASE COUNT         1083 a         1865 c         1804 g          997 t
ORIGIN      
        1 cggcgtgcga ccgctgcctg cggccacggt catgtagccg cggcgcctga gttcggggac
       61 gagctcggag ccgatctggc ctgccgcgcc gatcacgagg atgttcttca tgggccctcc
      121 ttccagagtg ttgtccgaat caggatagca cgggtgcgcg gaggcacagg ctgttcagaa
      181 cgtacaagga aaaaggccgc tcctgcgcct tgtggcgcgg aagcggcctt gtcggagcgg
      241 gctccgggag agcttagctc gcgagaacga tcagcttcat cacgaagagc gagacgaagg
      301 ccgtgacgat cgggagagcc atgatcttga agaggttcga gcccttgatg ccgatcacgc
      361 cgaggcagcg gcccatgtac tggacctggc tgcccatgag gtagatcgac ggggcgacga
      421 tggcgacgtg ctcgccggtg agaagccctt ggtcaaagag cgagacggca acacccacgc
      481 cgccgcccat ggacatccaa ccgccgagga gaacggcggc ggcttcgccg gggaggccga
      541 agatggccat gatcggggcg aacagttcgc cgaagatcgt gaggaggccc gagtggttga
      601 ggaccttgat gatcacgaac gccatcagga cgttcggaat cgtggaggtg acggcgatgt
      661 tccagccttg gacggcgccc ttcacgaaca cgtcggtgac catcggatta gcagattcat
      721 gtccggacat ttttgttcct tcttctgttt ttaacgttcg tggttacttg cgggaggcgc
      781 ggatctggag gaggcgcatc atgttggcgg agagaacctt gccgagcagg atgacgccga
      841 ggcacaggcc caggctgacc ggaacggcga gcgtgccgtc ggcagcgacg agcgtgaaga
      901 gtacggcgcc ggaggagaag aagttcgtga tcggtgcgtc ggccgtcatc tggaacgcgg
      961 cgaacgtgtt ggtctcggat tccgtgagct cgccggcgtc cttgagctgg cgggtgaggg
     1021 cggcaccacc gtcggtcgac tgcatcgagg cgatgagggc gagagcggcg gaaccgggga
     1081 tgccgatgag cggacgcaga accggggtga ggatcttgcg ggcggccttg agggcgccgt
     1141 agtgctcgaa gacggtgatc atcgcaaggg cgaacatcac cgtcggaacg agggaaagag
     1201 cgaacatgaa gccgtcgatg gcgccggagc cgcccttgcc tcggaagttg gccatggccg
     1261 tcttgatggc gtcgccctgt tcggtgacgc cgcttacgag tcggccgaag ccgccgttaa
     1321 cggtcgtgaa gtcgaagacg ccccaccact gagaggaggc gcagaggccg gagaagaaga
     1381 cgacggcaaa tgcgagcgag agatacgcgc cgatgccgac cttttcattg acggcctgtt
     1441 cggtcgtttt ttcgggggtc atgatacaag tcctttggtt gttttggagt aagggggcgg
     1501 tcttgagccg cttatttcct tgctgcgcgg gaagggaaag cttcgcgctc gcctgcaaat
     1561 cataatggag acgccttaaa acgtcgctaa gcgtttaccc tctgccactg ggcatgcgga
     1621 ggagatgccg cttttggtga tccttcggac gtgttccgtg cgcgcgactg cttgaggtgt
     1681 tcctgccggt tgccgattgg aaacgcctat cttactgatg aaaaggcgct ttcggattgc
     1741 tgtgtgcgag gccggtagtt gtaaagaagc ttgcccgcga gtcgggcatg caacaaaagt
     1801 cattaaaatg aaagattcag ttcgggagtc ttcagcagag tcctgcgcag acccgccgac
     1861 acgccattca aagacaaaca ttgctgactg gagcaacgca tatgccgctc aatgaccccc
     1921 gctggggccg ctcctccgac gaggagccgc gcagggacga tcgttccgcc gacgaagagc
     1981 cgccccgtcg tcccgacgag gacgatgacc gacgtgacga caaccgccgc gacgaccgcc
     2041 gccgcggcga tcgacgcgat gacaacgacg ggatggacga tctcgaccgt ctctggaagg
     2101 aattctccga acttctgcac ggcgcaacgg gccgcgacag gaaggaggac cgccgtcatc
     2161 ccgaccttcg cagccgagac gacttctccc gcgacgatgt ggatgagcgt gatgaccgct
     2221 acggtcgtga cgaggatcgt gacgacgagc gtgtcgagcg tcctcaggcc cgccgctacg
     2281 gcgacgacga ccgtcgtgac gaccgcgccg accgcccgac gggcgcccgc ggcatcttcg
     2341 gcggcctcgg cggcatggga ggcctcggcg gcttcggcgg caagcgtccc aatctgcctg
     2401 cgggccgcaa gggggcgggc atcggcatgt ccgtgatcgt gggcctggcg gttctcgcct
     2461 ggggcgccac gggcttctac atcgtgcccg aaggccagag cggcatcgtg accacgttcg
     2521 gtcgctatgc cgagacgacc gccgccggct tccgctggca tctcccgatg ccgattcagg
     2581 acgtgcagat cgtcgacgtc tcgagcgttc gcacgaccga aatcggcttt gccggccgct
     2641 ccgaccgtct gcgcgaagcc ctgatgctca ccgacgacga gaacatcgtc gacgtgcagt
     2701 tcaccgtcca gtaccgcatc aagccgggcg acggcgcgcg cgactacgtc ttccgcatcc
     2761 gccagcccga cctcaccgtg acgcaggccg ccgagtccgc catgcgcgag gtcgtcggcc
     2821 gcaaggcaat ggacagcgtc ctcttcgaaa gcaaggccga gatcgccgag gccgtcaaga
     2881 agtcgatgca ggacatgctc gatcgctact cctccggcat tgaggtcatg agcgtcgcca
     2941 tccagaacgc ccagccgccc cagcaggttc aggccgcctt caacgacgcc gtcaaggctg
     3001 gtcaggaccg cgagcgctcg atcaacctcg gcgaggccta ccgcaacgcc gtcatcccga
     3061 aggccgaagg taccgccgca cgcctcaagg aagaggcgct cggctatcag gcccgcatcg
     3121 tcgagaccgc ccgaggcgat gccgaccgct tctccgccct gcagtcccag tacgccaggg
     3181 ctcctgaagt cacgcgcgac cgactctacg tcgacaccgt gcgcgacgtc atgaagaacg
     3241 tcaccaaggt ctacgtcgac gccaagggcg gcaacaacct gctgtacctg ccgctcgaca
     3301 agatcgtcaa caacacgaag tccgacgcca tgactcaggc ccagaaggag gccgccgcgc
     3361 tcgccgacca cggcgtcacg gccgcccaga gcgccgtcga gacgacgggc tccgcccagc
     3421 cgacgacttc ttccgccgct cccggcgacg aatatcagcg catgctccgc gaccgccgct
     3481 aaaggacgac agccatgaag aaactctcca cgcttttgat cgccgtcctc gtggcggccg
     3541 gcctgctccg catgtgcgtc tacaccgtga acgagcgcga gtacgctctt gtcttcgccc
     3601 tcggcgaact caagaccgtg atctccgagc ccggccttca tgtgaagctc ccgccgcccc
     3661 tgcagaacat cgtctacctc gacaagcgca tcctcaacct tgatgcggcc ggcgccgacc
     3721 tcgtccagac gtccgaaaag aagaaccttc tgatcgatac gttcgtcaag tggcgcatcg
     3781 aggatccgcg cctctactgg gtctccttcc agggctccga acccgctgcc gcgaaccgcg
     3841 tctccgcact tctgcgcgac gtgctcaacg tggttgtcaa caagcgcacc gtcaaccaga
     3901 tcacgtcctc cgagcgtgac aaggccatgg tcgaaatctc gcagctcctt caggaacgcg
     3961 tgcgcgacgt cggcatcggc atcgttgacg tgcgcatgaa gcgcgttgac ttcacgcccg
     4021 agatctccga gtctgtctac cgccgcatgg aggccgagcg caagcgcgtc gcgagcgagg
     4081 aacgctccaa gggcgccgcc caggccgagc gcatccgcgc ctccgccgac cgcgagagcg
     4141 aggtgatcct cgcccaagcc tatcgtgatg cgcagaaggt caagggcgaa ggcgatgctc
     4201 aggccgccga gatctacgcc gaggccttcg gccgcgatcc cgagttcgcc cgcttctacc
     4261 gcagcctgca ggcctaccgc gacagcttcg ccgacaagaa cgacgtgatg gtcgttgatc
     4321 cgtccgccga cttcttccgc tacttcaagt cggaaaaggc gcaatgagca ggctcgacgt
     4381 catgatgctc gcgctcgggt gggtcctcgt catcgagggc ttcacgccga tggtggcccc
     4441 gggcttctgg aaggagtcga taagccgatt cctcgacgag cccgaccgcg cgctgcgcat
     4501 cacgggcctt ctgctcgtcg cactcggact ggcgatcatc tggatcgtct gatccgctga
     4561 ttcaggatcg ggtcctcggc tctcaaagcc gttcgagccg agttgaattg ggacactgtc
     4621 cttcaggggg cggatgcttt gaggtatccg ccccctgagt ttgttgcgct cggcgaaggc
     4681 gtctaagtgg tttttcggac cctgattcgg gagcggcgcg cgcattttgc taaacttacg
     4741 attcgtgtga gagacgacgc cccgccggcg ggtaccaagc cctgtcggct tccgccgtgc
     4801 ccgcagactt tgcggtcgcc tgcagcgggc ctgcgtcgcc gctctcaatc catttcaagc
     4861 ccgcagctgc ttccctgctt ctgcgggcag ccaatgggcg gctcggatcc gccgtcaatc
     4921 tgttcattaa gggtaatctc atggccaaga atgtagttgt ggtcggcgct cagtggggcg
     4981 acgaaggcaa gggcaagatc gttgactggc tgaccgaaaa ggtcgccggc gtcgtccgct
     5041 tcaacggcgg tcacaatgcc ggtcacacgc tcgtgatcaa cggcaagaag accattctcc
     5101 gtctcattcc gtccggcatc atgcatgcca cgtccgtctg ctacctcggc aacggcgtcg
     5161 tcctctctcc ggaagccttc ttccgcgaag tcgacgaact caacgccatc ggcgtgccca
     5221 acgttgagaa gcgtttgcgc gtctccgcca acgccgccct catcatgccg taccacgtcg
     5281 cccttgacca tgcccgcgaa gccgctgccg gcgacaagaa gatcggcacg acgggccgtg
     5341 gcatcggccc cgcctacgag gacaagattg cccgccgcac gctgcgcgtc gccgacctct
     5401 tcgacaagga acgcttcgtc gagcaggtcc gctccgtcct gaagtatcac aacttcgttc
     5461 tcgaaaacta ccttggtgcc aagccgcttg acgccgacga agtcatcgac cacatgctct
     5521 cctacgccga gcgcctccgt ccgatggtct gcgacgtctc cgccgaactc catcagcagt
     5581 gcgccgaagg caagcagttc ctcttcgaag gcgcccaagg ctcgatgctc gacatcgacc
     5641 acggcacgta cccgtacgtc acgagctcca acaccgtcgc cggctctgct tgcgccggcg
     5701 ccggcgtcgg tccgcacatg ctcaactacg tcctcggcat caccaaggc
//
LOCUS       DBMDTI010000121         5724 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3485_length_5724_cov_3.302863, whole genome shotgun sequence.
ACCESSION   DBMDTI010000121 DBMDTI010000000
VERSION     DBMDTI010000121.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3485_length_5724_cov_3.302863"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            393..959
                     /locus_tag="ACZPB6_06600"
     CDS             393..959
                     /locus_tag="ACZPB6_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184170.1"
                     /translation="MYKPERGAIVWRKHVMTYMKPDTGYMKEALASDRRVKYCRIVRR
                     TLKGVRRWFVQLVVEGLPPVRKVYASKCEVVGIDPGSSRIAYFHERHAAIVEVAPHVD
                     LKEPKIRLLQRRIDRSRRANNPDGTVKKGSSTWNTSNRGRRTAARSKLRKTITDLFNA
                     ASDGRQTGGEWVSLWSISNARLKAPAAR"
     gene            1540..4401
                     /gene="glnE"
                     /locus_tag="ACZPB6_06605"
     CDS             1540..4401
                     /gene="glnE"
                     /locus_tag="ACZPB6_06605"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HIR1184171.1"
                     /translation="MPSLTLEDVVRFSPFVARSLSAMHPGEDARACLAHFERIVEETF
                     PREAMEAEAAAAADSQTLRMKLREMRRRLIVNIIGRNATGRIDYFEVVRLMSDFAETA
                     VTATVKVNARELAERCGVPYGMSGRPQDLMVVGMGKLGGRELNVSSDIDLIFLFDEEG
                     ETRATPEFPNARRTLTVQEFYERLARRVIPALNDIEGPGFVFRVDMRLRPNGESGPIV
                     CSTDMLEEYLYTQGRDWERFAWLKGRIINEPVFSAPEDFEQQKRNVSSLVRPFVFRKY
                     LDFNAISSLTRLHEMIRAETARRELARGGTCINVKLGRGGIREIEFLTQTLQVIRGGR
                     DPLLRGRETLAMLEVLSGDGGISAEMAARLSEHYVFLRNVEHAIQYVNDEQTQRLPKE
                     GEGLTAVAGLLGMPADELWSRLEGVREYVAAAFDSVFQVHEPAADTADWPTGWETGTS
                     SVSEALTGKLRSLGYGDDVDELVSRIMRLASARAGRSLSDEARKRLQSLIPVVAEGCP
                     EWLPKDGPRVVPAVEVFSRYLKLLEAIAGRSTYVALLSQYPKAAERVGRVLAASRWTA
                     DYIVRHPIILDELVDGRIHEMDDFTPVDWSEWADRLHRALVEADGDQERQMNALRDAH
                     HSAVFRLLIADLDGRFTVERLADHLSALADAVLEEVLDLAWVSMTKKHCDRPKFAVIG
                     YGKLGGKELGYDSDLDLVFIYDDPDLEADLTYSRLVRRMMSWLTIQTSSGKLFDVDLR
                     LRPNGDSGLIVSSFDMFSRYQRNADGNGAWFWEHQALTRARFVAGDADVGKRFEDERR
                     EILMMPRTQDEARASVLEMRRKMLDGHPNRTALFDIKHDRGGMVDVEFIVQLLVLTHS
                     AEHPELVNNFGNILLLEKAADLGLTDPAVSAPAVRAYRRYRALQHEIRLNAGEGVPVR
                     VPADMIEAEREAVLKLWRTVFETDAPLREGDTFDRSETR"
     gene            4441..5415
                     /locus_tag="ACZPB6_06610"
     CDS             4441..5415
                     /locus_tag="ACZPB6_06610"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HIR1184172.1"
                     /translation="MPIVVPDYKAPSWCPGGHLQTVIPAKFMPRPAIEYRREKVELPD
                     GDFMLWDWAVPEPADPSAPVLVHFHGLEGSSRSHYAEALMAACTERGWRGVVAHFRSC
                     GGEMNRLPRAYFAGDSDDCEWVLRTVHERFPDAELRAVGVSLGGNQLAKYLGDRGTDA
                     GFLTAAVSVGAPLDLVAGSERISKGVNTFYADMFLHTLRQKLELKAKQFPDVIREEDV
                     KACRTMFDFDNTYTGPIHGFRSAMEYWQKCSAKTVLPDVRVPLLLLNAKNDPFLPPWC
                     LPTAGEMSAFVTGDFPEEGGHIGFPQGRVPGNINYLPQRIMRFFDTGS"
     gene            5504..>5724
                     /locus_tag="ACZPB6_06615"
     CDS             5504..>5724
                     /locus_tag="ACZPB6_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HIR1184173.1"
                     /translation="MPFSMSGRTVGLISLILFILTIPAGNWVVMNVGTVCPPDSPCLI
                     PVWPGIMSPSAVLFAGLALILRDAVQSLLG"
BASE COUNT         1105 a         1768 c         1819 g         1032 t
ORIGIN      
        1 tcagcagaat cgttttctcg aaaaggcgtt tgagttagga cgtacgcatc tacaatgcga
       61 cgctcgggac agctctcggg tgtctgcagc gcatgcgtga aacgcaggag tggcgggtgg
      121 cacggaacat gcccaagggg aaagcacgca cgaaagcgtt cagtgctgtt cactaagcct
      181 ttggactaac cgagttcggc cttacgatca ttgcgaataa tcatcgcaag gcgagcggtc
      241 gaaaagacat tggcgctcat gaagcgcaaa gtattggcaa agcggtccgg agggctctac
      301 agcgtcacat gttccggaaa gccggcagac ctcgcttcaa atccttcagg cgtggactca
      361 attccatcga aggcacgaac aatcaggaaa tcatgtacaa gcccgagcgt ggagccattg
      421 tctggcgcaa gcacgtgatg acgtacatga agcccgacac cggctacatg aaggaggcgc
      481 tcgcctccga ccggcgcgtg aaatactgcc gaatcgtgcg caggacgctc aagggcgtca
      541 ggcgctggtt cgtgcagctc gtggttgaag gcctgccgcc cgttcgcaag gtctatgcgt
      601 ccaaatgcga ggtcgtcggc atcgacccgg gttcaagccg gattgcttat tttcatgagc
      661 gtcatgcggc catagtcgag gttgcacctc acgtggatct gaaggagccg aagatccgtc
      721 tcctgcagcg aaggatcgac cgttcaagac gagcgaacaa tcccgacgga accgtgaaaa
      781 agggttcgtc gacttggaac acaagcaatc gcggacgcag gacggcggca cgatcaaaat
      841 tgagaaaaac aattaccgat cttttcaacg ctgcttcgga cggtcgacaa accggagggg
      901 aatgggtgag tttgtggagc atctcaaacg caaggctgaa agcgccggct gcgaggtgat
      961 cgaacacaat gcctataagc tgaagatgtc gcagtacgat ccgcaaacgg atgcgtaccg
     1021 caagaagccg ttgaaggaaa gatggccccg ctggggaaac accggcacgc tcgtgcagcg
     1081 agacgccatg agtgcgtttc cggcgtgcca tgcaactgaa aaaggacacg accgagccct
     1141 tctccttgag aagtggacga ctgcggaagc actgttgagc ggcagcggtc tgtgtcgtca
     1201 tgaaccttgt agcgaccctg aagtatcgaa agacgcttcg aggctcacga agccgaattg
     1261 cgggagcaag gcggaacggg aaagaggtct gccgcgcaca ctttgtacgg acggtgacgt
     1321 ttaggcggct ttgcccccta aaagagctgt ctgacggtca aagtgaattc tctgtcttca
     1381 gacaggggag cttcaaaggc attgaactgc agggcgaagg acctccaaca gcggtgcggc
     1441 gcccgacact ctccttgaga ccgtgccgat ttggctagaa tgggctgacg gggtcgcccc
     1501 atgaattcaa tagatacttt cctgctttcg gagtccttca tgccctcact gactcttgag
     1561 gacgtcgtcc gcttctcgcc ctttgtcgca cggtcgttgt cggccatgca tcccggagag
     1621 gacgctcggg cgtgcctcgc gcattttgag aggatcgtcg aggagacgtt cccgcgggag
     1681 gccatggagg ccgaggcggc ggccgcggcc gacagccaga cgctgcgcat gaagctcagg
     1741 gagatgaggc gccgcctcat cgtgaacatc atcgggagaa acgcgacggg tcgcatcgac
     1801 tacttcgaag tcgtgaggct catgagcgac ttcgccgaga cggccgtcac ggcgacggtg
     1861 aaggtcaacg ccagagagct ggccgagcgt tgcggcgtgc cgtacggcat gtctggacgt
     1921 ccccaagacc tgatggtcgt gggcatgggc aagctcggcg gccgagagct caacgtctcc
     1981 tccgacatcg acctcatctt cctcttcgac gaggaaggcg agacgcgggc gacgcccgaa
     2041 tttccgaacg cccgccgcac gctcaccgtg caggagttct atgaaaggct ggcaaggcgc
     2101 gtgattcccg ccctgaacga catcgagggg cccggcttcg tcttccgagt cgacatgcgc
     2161 ctgcgtccga acggcgagtc gggtccgatc gtgtgctcga cagacatgct cgaggagtat
     2221 ctctacacgc agggccgcga ctgggagcgc ttcgcctggc tcaagggccg catcatcaac
     2281 gagcccgtct tctcggcgcc tgaggacttc gagcagcaga agcggaacgt ctcgtccctc
     2341 gtgcgtccgt tcgtcttcag aaagtatctg gatttcaacg ctatttcgtc gctcacgcgc
     2401 ctgcacgaga tgatccgcgc cgagacggcc agacgcgagc tcgcccgcgg cggcacgtgc
     2461 atcaacgtca agctcggacg cggcggcatc cgcgagatcg agttcctcac gcagacgctt
     2521 caggtcatcc gcggcggacg tgatccgctt cttcgcgggc gcgagacgct cgcgatgctc
     2581 gaggtgctct cgggcgacgg cggcatttcc gccgaaatgg ccgcgaggct ttccgagcac
     2641 tacgtcttcc tgcgcaacgt cgagcatgcc attcagtatg tcaacgacga gcagacgcag
     2701 cgcctcccga aggaaggcga gggactcacg gccgtggcgg gcctcctggg catgcccgcc
     2761 gacgaactct ggagccgtct tgagggcgtg cgcgagtatg tcgccgccgc cttcgacagc
     2821 gtctttcagg tccatgagcc cgccgcagac acggccgact ggcccacggg ctgggagacg
     2881 ggcacgtcga gcgtgagcga ggcactcaca ggaaagctca gatctctggg ctacggcgat
     2941 gacgtcgacg agcttgtctc gcgcatcatg cgtcttgcct cggcccgtgc gggccgaagc
     3001 ctctccgacg aggcgaggaa gcgccttcag agcctgattc ccgtcgtggc cgaaggatgc
     3061 ccggagtggc ttcccaagga cggcccgcgc gtggtgccgg cggtcgaggt gttctcccgc
     3121 tatctgaagc tgcttgaggc gatcgcgggc cgctcgacct acgtggcgct cctctcgcag
     3181 tatccgaagg ccgccgagcg cgtcggacgc gtgctggcgg cgagccgctg gacggccgac
     3241 tacatcgtgc gccatccgat catccttgac gagctcgtcg acggacgcat ccacgagatg
     3301 gacgacttca cgcccgtcga ctggagcgag tgggcggaca ggctgcatcg tgcgctcgtc
     3361 gaggccgatg gcgatcagga gcgccagatg aacgcgcttc gcgacgccca tcactccgcc
     3421 gttttcaggc tactgatcgc ggaccttgac ggccgcttca cggtcgagcg cctggcggac
     3481 catctctcgg cgttggccga cgccgtgctc gaggaggtgc tcgatctggc ctgggtcagc
     3541 atgacgaaga agcactgcga ccgtccgaag ttcgccgtga tcggctacgg caagctgggc
     3601 ggcaaggaac tcggctacga cagcgacctc gatctggtct tcatctatga cgatcccgat
     3661 ctggaggccg acctcacgta cagccgcctc gtgcgccgca tgatgagctg gctgacgatt
     3721 cagacgtcgt cgggcaagct cttcgacgtg gacctgaggc tgcgtccgaa cggcgacagc
     3781 ggcctcatcg tgtcgtcctt tgacatgttc agccgctatc agaggaacgc cgacggcaac
     3841 ggcgcctggt tctgggagca tcaggctctc acgcgcgccc gcttcgtggc gggcgatgcg
     3901 gacgtgggca agcgcttcga ggacgagagg cgcgagatcc tgatgatgcc gagaacgcag
     3961 gacgaggccc gcgcgagcgt gctcgagatg cgcaggaaga tgctcgacgg gcatcccaac
     4021 cgcacggcgc tcttcgacat caagcacgac cgaggcggca tggtcgacgt ggagttcatc
     4081 gtgcagcttc tcgtgctcac gcactccgcg gagcatcccg agctggttaa caacttcggc
     4141 aacatcctgc ttcttgaaaa ggcggccgac ctcggcctga cggatcccgc cgtttcggcg
     4201 cccgccgtga gggcgtatcg acgctaccgc gccctgcaac acgagatcag gctcaatgcg
     4261 ggcgaaggtg tgcccgtgcg cgttcctgcg gacatgatcg aggccgaacg cgaggccgtg
     4321 ctcaagctct ggagaacggt gtttgagacc gatgcgcctc tgagagaggg cgatacgttc
     4381 gatcggtccg aaacgcgcta gactgtcgga cgatcgattt tccatcaacc ggagcatgtg
     4441 atgcccattg tcgttcctga ttacaaagcc ccgtcctggt gtcccggcgg tcatcttcag
     4501 accgtcattc ccgccaagtt catgccgagg cccgcgatcg agtacaggcg cgagaaggtt
     4561 gagcttcccg acggcgactt catgctctgg gactgggccg tgcccgagcc cgccgatccg
     4621 tcggcgcccg tacttgtgca cttccacggg ctcgagggct cgagccgcag ccactatgcc
     4681 gaagcgctga tggcggcctg caccgagcgc ggctggcgcg gcgtcgtcgc acacttcaga
     4741 agctgcggcg gcgagatgaa caggcttccg cgcgcctact tcgcgggcga cagcgacgac
     4801 tgcgaatggg tgctcaggac cgtgcacgaa cgcttccccg acgcggagct cagggccgtg
     4861 ggcgtgagcc tcggcggcaa ccagctcgcc aagtacctcg gggaccgcgg aaccgacgcg
     4921 ggcttcctga cggcggccgt ttcggtgggc gcaccgctcg acctcgtggc gggcagcgag
     4981 cgcatcagca agggagtcaa cactttttat gccgacatgt tccttcacac gctccgccag
     5041 aagctcgagc tcaaggcgaa gcagtttccc gacgtgatcc gcgaggagga cgtgaaggcc
     5101 tgccgcacga tgttcgactt cgacaacacg tacacggggc cgatccacgg gttccgctcg
     5161 gcgatggagt actggcagaa gtgctcggcc aagacggtgc tccccgacgt gcgcgtgccg
     5221 cttctgctgc tcaatgcaaa gaacgatccg ttcctgccgc cgtggtgcct tccgacggcg
     5281 ggcgagatga gcgccttcgt cacgggagat tttcccgaag agggaggaca catcggattt
     5341 ccacaaggac gggttccggg caacatcaat tatctgccgc aacgtataat gcgcttcttc
     5401 gacaccggca gctgaaccgt ctgaccgggt gtcagcctct cgtcaattcc ttcttttggg
     5461 caagcgccgg ctttccgacc ggtagcccct ggagacccgc aagatgcctt tcagcatgtc
     5521 cggccgcacc gtcggcctca tcagcctcat tctcttcatc ctcaccattc ccgcaggcaa
     5581 ctgggtggtg atgaacgtcg gcaccgtctg tccgcccgac agcccctgcc tcattcccgt
     5641 ctggcccggc atcatgtcgc cgtccgccgt gctcttcgcc ggtcttgcgc tcattctgcg
     5701 cgacgcggtc cagagcctgc tcgg
//
LOCUS       DBMDTI010000122         5680 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3513_length_5680_cov_2.020075, whole genome shotgun sequence.
ACCESSION   DBMDTI010000122 DBMDTI010000000
VERSION     DBMDTI010000122.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3513_length_5680_cov_2.020075"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="ACZPB6_06620"
     CDS             <1..1296
                     /locus_tag="ACZPB6_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184174.1"
                     /translation="KGSAPVSPAPKSNVAVEPGEQFYERALRRRIRSWPDVSAAGFLT
                     VELSQYLKGTGGATAEAVMAALLRLGHDGLVANGRRLASRLDTLTVSEARDVLEACGL
                     AAERHARAMTRACEKNPEGISEREARERALCPAVTARLTGTYHAIPWDARHDGHLASR
                     EVSRAAGLVRWAHALTSERSSRVHLAVGRSLLFGRGAADVWKHRRPLVFIDRINAYTV
                     KLVTDCWTADPQTAVVYAGDSVPATNPFDAWEIPLVVDTRGFLRALLAVDKTPCLIEP
                     RLGGFTVTCATGRVDPKGFRDGEKLAPWMKTLAEIGLINAYDEAARAGNVPAFTGAEA
                     CVWAERFKSLLSVTLALDNSARRGLEVDEKALEAAARQRENYVAVRNEMTSILEKLAD
                     GARELREAELAQRAERRTKQREERKAQRAARLARREENA"
     gene            1296..2339
                     /locus_tag="ACZPB6_06625"
     CDS             1296..2339
                     /locus_tag="ACZPB6_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HIR1184175.1"
                     /translation="MRPLSFLLAGAAMFVAALTVNAATVCQGEILNPVTDVCWSCVFP
                     VKIGGKASLTTSVLPDPDTGAGGPFCTCGKGLNLRAGVTFSFWEPLRTAEIVRHAGCL
                     PSMGGVNMGDMGLRISDHVKVHSHEPDGSMKRHTDFRQVHWYATPWLFILEALLDNGC
                     LDHQTFDLAYMSELDPLWDDTLSSFILNPDAALFANPIAQGACAADCTSASSGLPQNS
                     LYWCSGCQGNVFPLTGWSASLTSHTAVWQLMTHRFAMKLSREGLLLAAHGSKGLCESF
                     YEPLMRKDVWRTQLVWPAHSGKNTDGSCCSPLGRTTVVSGSGKSWSAGGEDGVVLVWR
                     RRDCCSMSAKWGG"
     gene            2642..3268
                     /locus_tag="ACZPB6_06630"
     CDS             2642..3268
                     /locus_tag="ACZPB6_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184176.1"
                     /translation="MTTLIADHPVQTSSDPDFEEILKANARIAVESGEWSRRVKRKEE
                     TLIQWMRSPVFPEFRPMAVKTTLIRRPTGLSVEDIRHAELFNDVSRLLLFFDSRAGGQ
                     VNFAMRMLSTASFAIRPVVAAGSVAFAQEKLGMRVWADQGSVLTRRLGLNHWPALVKL
                     TPSEISVWTPALNADGIPDSPVPAGLASKESIDLSVPAVRPAASKTLH"
     gene            3282..4028
                     /locus_tag="ACZPB6_06635"
     CDS             3282..4028
                     /locus_tag="ACZPB6_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184177.1"
                     /translation="MNFVSALGAAALSCLLLPAAFASGPAGTALEPSSATIAPKVREV
                     EKAPSTELAAAPSASSSPEAFSDSKSDNSNTWRPRITILLDTGMTSSLVETAAEELKL
                     VGDAADVEVLVRGLPVTRTPTGYRTDFEATSERLAPLVDAGFGAAIHPERFAFLLALA
                     EEGLEAGGRLDAKTEEVYEVMTAAPTAPAVLLTIGRSVYAVSGTASIVSALSQWRRTL
                     AEAVIDESDRTRVAALQTVLDGTPLGRIAP"
     gene            4079..5296
                     /locus_tag="ACZPB6_06640"
     CDS             4079..5296
                     /locus_tag="ACZPB6_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184178.1"
                     /translation="MTRTVPVRCLISLMTFALGLSIALPSHAVTVAEAVAAARNTQLP
                     DIDGIMANHDGVLDAVGGGSTEGLDDVKNLWADGKGTLTGSAEATVIECRGKTDTTCR
                     AIQVLDKGFPERPVIGEDVLAGRDEIVNNPTVTPDDNDTCKDVTIEADPVEVTKTCRA
                     GGWWNEKACRIGPVEIGKVTAKYFACAEGECTSSERSCLTPVSGKPASSVWTERCFFG
                     KESSAASGTVTEETSAVARAIFSGVCTSTDATSETVKCSEVLESNNDHGCVLGDKTTA
                     RVNGEYSLSHDPCSDGDVLIGQHTCGNSSYLAIQVKGFKSTRISLGYTLRNIAHRTNS
                     SCKADYEWTKRECSGPTCTAKLGARVYYNGYMTGSMRLTLTYVNESYYIKEEWVDHCA
                     SIRPKTAATAEGS"
     gene            5298..>5680
                     /locus_tag="ACZPB6_06645"
     CDS             5298..>5680
                     /locus_tag="ACZPB6_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184179.1"
                     /translation="MRRLFSLLPLLLPLPNLVQAADPVCTLENRVCAENNNSGKCIRY
                     ENTFTCVTVNPAGSRCTVDANPTLTDCTLSAPVCSAESGGICLETTTDFVCKSEPSGE
                     GIRIDDTSVTVSYSKETKGEIPAGC"
BASE COUNT          993 a         2022 c         1645 g         1020 t
ORIGIN      
        1 aagggctccg cgcccgtctc gcccgctccg aagagcaacg tcgccgtgga gcccggagag
       61 cagttctacg agcgtgcgct ccggaggcgc atccgctcct ggcccgacgt gtcggccgcg
      121 ggctttctga cggtcgagct ttcgcagtac ctcaagggca cgggcggtgc gacggccgag
      181 gccgtgatgg cagcgctcct gcgtttaggg cacgacggcc ttgtggccaa cggcaggcgt
      241 ctggcctcaa ggctcgacac gctcaccgtt tccgaagccc gtgacgtgct cgaagcgtgc
      301 ggccttgcgg ccgaacgcca tgcccgcgcc atgacaaggg cctgcgagaa gaatcccgag
      361 gggatttccg agcgtgaggc ccgcgagcgc gcgctctgcc ccgccgtcac cgcgaggctc
      421 acgggcacgt accacgccat tccctgggat gcccgtcacg acggccatct ggcctcgcgt
      481 gaagtctcgc gtgccgcagg actcgtgcgc tgggcccatg cgctcacgtc cgagcgttct
      541 tcgcgcgtgc atctggccgt cggccgttcg cttctcttcg gacgaggtgc tgccgacgtc
      601 tggaagcacc gtcgtccgct cgtcttcatc gaccgcatca acgcctacac cgtgaagctc
      661 gtcaccgact gctggaccgc cgatccgcag actgccgtcg tctatgcggg cgacagcgtc
      721 cccgcgacga atccgttcga cgcgtgggag attccgctcg tcgtcgatac gcgcggcttt
      781 ctgcgtgcgc tccttgccgt cgacaagaca ccctgcctga tcgaaccccg cctcggcggc
      841 tttacggtga cctgcgccac gggccgcgtc gacccgaagg gctttcgcga cggcgagaag
      901 ctcgccccct ggatgaagac ccttgccgaa atcggtctca tcaacgccta tgacgaggcc
      961 gcaagggcgg gcaatgttcc cgccttcacg ggagccgaag cctgcgtctg ggccgagcgc
     1021 ttcaagtcgc tcctctccgt gacgcttgcg ctcgacaact ccgcccgccg cgggcttgaa
     1081 gtcgacgaaa aggcactcga agccgccgca cgccagcgcg agaactacgt ggccgtgaga
     1141 aacgaaatga cctcgattct cgaaaagctc gccgacggcg cacgcgaact ccgtgaggcc
     1201 gagcttgccc aaagggccga acgcaggaca aagcagcgcg aagaacgcaa ggcccaaagg
     1261 gccgcccgtc tcgcccgacg tgaggagaac gcctgatgcg cccgctttcc tttcttcttg
     1321 cgggcgcggc gatgttcgtc gcggccctca cggtgaatgc cgccacggtc tgtcagggcg
     1381 agatcctcaa tcccgtgacc gacgtttgct ggagctgcgt ctttcccgtg aagatcggcg
     1441 gcaaggcctc gctcaccacc tcggttctgc ccgaccccga cacgggtgcg ggcgggccgt
     1501 tctgcacctg tggcaaaggc ctcaacctga gggccggcgt cacgttcagc ttctgggagc
     1561 cccttcgtac ggcggagatc gttcgtcatg cggggtgtct tccctcgatg ggcggcgtga
     1621 acatgggcga catggggctt cgcatatcag accacgtgaa ggtgcattcg cacgagcccg
     1681 acggatcgat gaagcggcac acggactttc gtcaggtgca ctggtatgcg acgccctggc
     1741 tcttcattct cgaggcgctc cttgacaacg ggtgtctgga ccatcagacg ttcgacctcg
     1801 cctacatgtc cgaacttgat cccctatggg acgacacgct ctcgagcttc atcctgaacc
     1861 cggacgccgc gctctttgcg aatccgatcg cccagggcgc gtgcgccgcc gactgcacgt
     1921 ccgcctcttc gggccttccc cagaactccc tctactggtg ctcgggctgt caggggaacg
     1981 tctttccgct cacgggctgg agcgcatcgc tcacctccca cacggccgtc tggcagctga
     2041 tgacccaccg ctttgcgatg aagctctcgc gtgaggggct gcttcttgcc gcccacggct
     2101 cgaaggggct ctgcgagtcc ttctacgagc ccctgatgcg aaaggacgtc tggcgcaccc
     2161 agctcgtctg gcctgcgcac tcgggaaaga acaccgacgg ctcctgctgc agcccgctcg
     2221 ggcgcaccac cgtcgtctcg ggctcgggaa agagctggag tgccggcggc gaggacggcg
     2281 tggtactcgt ctggcgacga cgcgactgct gctcgatgtc cgccaaatgg ggcggatgag
     2341 cccgacaaca ccctgcattc aagtcctttg cgggaattca taaacccaaa acccacagaa
     2401 agacaatgga gattctcatg aagcacgaat ccaacgacac gccgcccttt gcgcccgacg
     2461 accccatcag ccggagcctt ttcgtgaggc ccaacctcat cgagcgcctc tgcgcctctg
     2521 cggctcggcg cttcgcctct gcctgctcgt ctgcattccg gccgccgcat cggtcttcct
     2581 gatcagcctt gccgactcgg ccgaagccgc ctgcacgtcc gaaccttctc agcactacga
     2641 ggtgacgacg ctcattgccg accatcccgt gcagacctcg tcggaccccg acttcgagga
     2701 gatcctcaag gccaacgctc gcattgccgt ggagtcgggt gaatggtccc gccgtgtgaa
     2761 gcgaaaggaa gagacgctca ttcaatggat gcggtccccg gtctttcctg aattccgtcc
     2821 gatggccgtc aagaccacgc tcattcgcag acccacgggg ctttcggttg aggacatcag
     2881 gcacgccgag ctcttcaacg acgtgagccg cctccttctc ttcttcgact cccgtgcggg
     2941 cggccaggtc aacttcgcca tgaggatgct ctccaccgcc tccttcgcca ttcgtcccgt
     3001 cgtggcggcg ggcagcgttg cgttcgctca ggaaaagctc ggcatgcgcg tctgggcaga
     3061 ccagggctcg gtcctcacgc gccgtctcgg tctcaatcac tggcccgccc tcgtgaaact
     3121 cacgccgtcg gaaatctccg tctggacccc tgcgctcaat gccgacggga ttccggattc
     3181 gcccgtcccc gccggccttg cttcgaagga gtcgatcgac ctctccgtac ccgccgtgcg
     3241 ccccgctgca tctaaaaccc tccattaata ggaaagccgt catgaatttt gtgtccgcgc
     3301 tcggcgctgc cgccctttcc tgccttcttc tccccgccgc cttcgcttca ggacccgcag
     3361 gcactgctct cgaaccctcg tccgccacga ttgcgcccaa ggtccgtgaa gtggagaagg
     3421 ctccgtctac tgagctcgcc gccgcgcctt ccgcttcttc gtctcctgaa gccttttcgg
     3481 actcgaagtc cgacaactcg aacacttggc gaccccgcat cacgattctg ctcgacacgg
     3541 ggatgacgtc ctcgctcgtc gagacggcgg ccgaggaact caagctcgtg ggtgacgctg
     3601 cggacgtcga ggtcctcgtt cgaggtctcc ccgtgacgcg caccccgacg ggttaccgga
     3661 ccgacttcga agcaacgtcc gaacgtcttg cgccgctcgt tgacgcaggc ttcggagccg
     3721 cgatccatcc tgaacgcttc gccttccttc tggccctagc cgaagagggt ctagaggcgg
     3781 gcggcaggct cgatgcgaaa accgaagagg tttacgaagt gatgaccgcc gcgcccaccg
     3841 cacccgccgt tctcctcacg atcggccgca gcgtctatgc ggtctcgggc acggcctcca
     3901 tcgtctcggc cctttctcag tggcgtcgca cgctcgccga ggccgtcatt gacgaatcgg
     3961 accgcacccg cgtcgccgcc cttcaaaccg tactcgacgg aacgccgctc ggccgaatcg
     4021 cgccctgaac acccaaaacc gccccctgaa acccaatctc tacacttctt tgagagccat
     4081 gacccgtaca gttcccgttc gatgtctcat ttcactcatg acgttcgcgc tcggtctctc
     4141 cattgccctg ccctcgcacg ccgtgacggt tgccgaggcc gttgccgctg cgcgcaacac
     4201 tcagcttccc gacatcgacg ggatcatggc gaaccacgac ggcgtgcttg atgccgtggg
     4261 cggcgggtcg accgaaggtc tcgacgacgt caagaacctc tgggccgacg gcaagggcac
     4321 gctcacgggt tcggccgaag ccaccgtgat cgagtgtcgc ggcaagaccg acacgacctg
     4381 ccgcgccatt caggtgctcg acaagggctt cccggagcgt cccgtcatcg gcgaggacgt
     4441 gctcgcgggt cgcgacgaga tcgtcaacaa tccgaccgta acgccggacg acaacgacac
     4501 ctgcaaggac gtgaccatcg aagccgaccc cgtcgaggtg acgaagacct gtcgagcggg
     4561 cggctggtgg aacgagaagg cctgccgcat cggccccgtc gagatcggca aggtcacggc
     4621 aaagtacttt gcatgcgccg aaggcgaatg cacgtcctcc gagcgctcct gcctcacgcc
     4681 cgtctcagga aagcccgcat cctccgtctg gaccgagcga tgcttcttcg gcaaggaatc
     4741 ctccgccgcc tccggtaccg tcaccgaaga gacctcggcc gtcgcacgcg cgatcttctc
     4801 gggcgtgtgc acctccaccg acgccacgtc cgaaacggtc aagtgcagtg aagtcctcga
     4861 gtcgaacaac gaccacggat gcgtcctggg cgacaagacc accgcacgcg tcaacggcga
     4921 atacagtctc agccacgacc cctgttccga cggcgacgtc ctcatcggcc agcacacctg
     4981 cggcaactcc tcctatctgg ccatccaggt gaaggggttc aagtccacgc gaatctcgct
     5041 cggctacacc ctccgaaaca tcgcccaccg tacgaacagc agctgcaagg ccgactacga
     5101 atggacgaag cgtgaatgct ccggccccac ctgcacggcc aagctcggag cccgcgtcta
     5161 ttacaacggt tacatgacgg gctccatgag gctcaccctc acctacgtga acgagtccta
     5221 ctacatcaag gaagaatggg tcgaccactg cgcctcgatc cgccccaaga cggctgcgac
     5281 tgcggagggc tcctgacatg cgccgcctct tttcgctcct ccctcttctt ctcccgctgc
     5341 cgaacctcgt tcaggcggct gatcccgtct gcacgctcga gaaccgcgtc tgcgccgaga
     5401 acaacaattc cgggaaatgc attcgctacg agaacacctt tacctgcgtg acggtcaatc
     5461 ccgcgggctc tcgctgcacg gtcgacgcca atccgacgct cactgactgc acgctctccg
     5521 cgcccgtctg ttcggccgag tccggcggca tttgcctcga gaccacgacc gactttgtct
     5581 gcaagtccga accctcgggc gaaggcatcc gcatcgacga cacctccgtc acagtttcat
     5641 actcgaagga aaccaagggc gaaatccctg ccggctgcat
//
LOCUS       DBMDTI010000123         5626 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3542_length_5626_cov_4.196018, whole genome shotgun sequence.
ACCESSION   DBMDTI010000123 DBMDTI010000000
VERSION     DBMDTI010000123.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3542_length_5626_cov_4.196018"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /gene="dnaA"
                     /locus_tag="ACZPB6_06650"
     CDS             <1..1296
                     /gene="dnaA"
                     /locus_tag="ACZPB6_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR1184180.1"
                     /translation="TNWFEPIELVRWDSERGDMVLEAPASKVQTIRATYLKTIQDVAV
                     HLYGGQVKVTLQAVGALAGDVQTPAQPKGPSLEDIERRREETGLLPGLTFENYVNGNA
                     NQLAVAAAEHVATTMGMQYNPLYIYGGVGLGKTHLMQAIGHRYLDLHPKARVRCISAQ
                     DFISEYTTATRDSSSSRKALQMFDERYRNLDLLLIDDVQSFSGAKGSQAQFYRAFEAL
                     VPHNKQVVLTADTYTRNLKDIEQRLISRFSQGLSVAIEPPELEMRIAILLNKAKALGA
                     ALPEEVAMFIAKRLKSNVRELEGALQQVIAYQQFQPSAAKEITIDIAKHVLRDQFSVN
                     NNLITIESIQKTVADYYNIKVAEMHSKRRPANIARPRQIAMYLTKELTQHSLPEIGAN
                     FGGRDHTTVMHAVRKITQERQGNAELNHEIHVLEQMIKG"
     gene            1349..2461
                     /gene="dnaN"
                     /locus_tag="ACZPB6_06655"
     CDS             1349..2461
                     /gene="dnaN"
                     /locus_tag="ACZPB6_06655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR1184181.1"
                     /translation="MKNIQSVCENLLKPVKIVEGIVEKRQTLPILSNILIEQNGENVK
                     FSATDLDIQISTEAPVGVAGVDTRFTVGASKLASILTALPATNEVQFDLGDRGLTISS
                     NNGKFQLQTLSADEYPVLKESQWETTLFIPSRTLRYLLTMTAFAMASQDVRYYLNGVL
                     FVVEGKKLRTVATDTHRLACCEVDVDDENTPMQAIIPRKTVRELIRLLPEDDTPVRVQ
                     FTDLQVCFSFGNITFMSKLIEGRFPDYKRVLPSQDTNPNALELEREAISNALRRVGIM
                     TNEKFHGIRWILNGEGLQIQSTNSDHEEALERFDVPWTHDELDMGFNITYLLEVLGIL
                     KNKTVRFNFSTASGSVLITMPESQERFRYVVMPMRI"
     gene            2575..5106
                     /locus_tag="ACZPB6_06660"
     CDS             2575..5106
                     /locus_tag="ACZPB6_06660"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIR1184182.1"
                     /translation="MTEMDTNSDQQPTTASTYDEHSIQILEGLEAVRKRPGMYIGDTA
                     DGSGLHHLVYEVVDNSIDEALAGYATKIQVTIHTDNSISVVDDGRGIPSSIKWDDKHK
                     PKRSAAEIALTELHAGGKFDNNGYKISGGLHGVGVSCVNALSTWLRLIVRRDGEARLL
                     EFERGKVKNRLTETAKDPVTGAEVLISPMKLLGPTSRRGTEVHFLPDTQIFEQITEFN
                     YDTLLSRLRELSFLNSGVLIELKDQRTAHEAQLLTDKGLVAYVQFKNQSRTVLHQKIF
                     HAKETVYENGIPIEVEIAMQWQDGYSEVLSAYTNNIPQRDGGTHVTGLRMAMTRVLKN
                     YISANEIGKKSGSKKADIDPEGDDMREGLTCVLSVKVPQPKFSSQTKDKLVSSEVRPA
                     VEEVVSRELELYLEQNPADAKIICEKIVAAARAREAARKARDISRKSFSGGGLPGKLA
                     DCREKNPANSELFLVEGDSAGGSAKQGRDSKFQAILPLRGKVLNVEKASQDKLLDSEQ
                     IRMLTLALGTNIGKDFDISRLRYHRIIIMTDADVDGAHIRTLLLTLFYRQMPQLIENG
                     HVYIAQPPLYKVKKNQADKGVFLKDEREMSDYLLKIAMEDARLYRNKDAFDADTPVRG
                     SELETLVNEYLQSSDVIARLQGVIDKSVLVAIAAGVDINLENEFTSHHTAEVLQQELR
                     DPNVKVEAVYDEENEKHLLKVHRLKHGNYKTTNVTPEFTLSADYRKLQESAAMLRGII
                     GTGARVARGKNDQAVKNFGEAVNWLLKQAEKGIIRQRYKGLGEMTAEQLNLTTMDPSV
                     RRMLRVRIEDAASADAIFSMLMGDVVEPRRDFIETNALFAGNIDA"
     gene            5181..>5626
                     /locus_tag="ACZPB6_06665"
     CDS             5181..>5626
                     /locus_tag="ACZPB6_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HIR1184183.1"
                     /translation="MDNLKEKVVIAVSSRALFDLEEEHALFEEKGYKTFRDHQVRYQN
                     TPLRPGVAFPFVRRLLALNELYGDLAPFRVIALSRNSPETAQRFFHSCRHYRLPISAG
                     GFTSGHSTYPYLAAFNASLFLSANPENVRNAIAAGQPAGLVMPSPVG"
BASE COUNT         1226 a         1961 c         1514 g          925 t
ORIGIN      
        1 accaattggt tcgaaccgat tgagctggtg cggtgggaca gcgaacgcgg cgacatggtc
       61 cttgaagcgc ccgccagcaa ggtgcagacg atccgcgcga cgtatctgaa gaccattcag
      121 gacgtggccg tgcacctcta cggcggccag gtgaaggtca ccctgcaggc cgtcggcgca
      181 ctcgcaggcg acgtgcagac tcctgctcag cccaagggac cgtccctcga ggacattgag
      241 aggagacgcg aagagaccgg cctccttccc ggcctcacct ttgaaaacta cgtcaacggc
      301 aacgccaacc agctcgccgt cgcggccgcg gaacacgtcg ccactacgat gggcatgcag
      361 tacaacccgc tctacatcta tggcggcgtc ggtctgggca agacccatct gatgcaggcc
      421 atcggccacc gctatctcga ccttcatccg aaggcacgcg tccgctgcat ctccgcccag
      481 gacttcatca gcgagtacac gaccgccacg cgcgactcct cgagctcccg caaggcgctt
      541 cagatgttcg acgagcgcta ccgcaacctc gacctgcttc tcatcgacga cgtccagtcc
      601 ttctcgggcg ccaagggttc ccaggcgcag ttctaccgtg ccttcgaagc gctcgtgccg
      661 cacaacaagc aggtcgtgct cacggccgac acgtacaccc gcaacctcaa ggacatcgag
      721 cagcgcctaa tctcgcgctt ctcccagggc ctgtccgtcg ccatcgagcc gcccgagctt
      781 gaaatgcgca tcgccattct gctcaacaag gccaaggcgc tcggcgccgc gctgcccgaa
      841 gaagtggcga tgttcatcgc caagcgcctc aagtccaacg tccgcgagct tgagggcgcc
      901 cttcagcagg tcatcgccta ccagcagttc caaccgtcgg ccgccaagga aatcacaatc
      961 gacatcgcca agcacgtcct tcgcgaccag ttctcggtca acaacaacct gatcacgatc
     1021 gagagcatcc agaagaccgt cgccgactac tacaacatca aggtcgcgga aatgcattcg
     1081 aagcgccgcc ccgccaacat cgccaggccg cgccagattg ccatgtacct caccaaggag
     1141 ctgacccagc acagcctccc cgaaatcggc gccaacttcg gcggccgaga ccacacgacc
     1201 gtcatgcacg ccgtgcgaaa gatcactcag gaacgccagg gcaacgccga gctcaatcac
     1261 gagatccacg tgctcgagca gatgatcaag ggctgacaga agcacccgac ggaacccgat
     1321 tttttgaatt tttcaccagg tatttgttat gaagaacatc cagagcgtct gcgaaaacct
     1381 gctcaaaccc gtcaaaatcg tcgaaggcat tgttgaaaag cgccagacgc taccgattct
     1441 gagcaacatc ctgatcgagc agaacggcga gaacgtcaag ttctccgcca ctgacctcga
     1501 tatccagatc agcaccgagg ctcccgtcgg cgtcgcaggc gtcgacaccc gcttcacggt
     1561 cggcgcctcc aagctcgcca gcatcctgac ggctcttccc gccaccaacg aggtccagtt
     1621 cgatctcggc gaccgcggcc tcacgatcag cagcaacaac ggcaagttcc agcttcagac
     1681 gctctccgcc gacgaatacc ccgtcctcaa ggaaagccag tgggagacga cgctcttcat
     1741 cccgtcccgc acgctacgct acctgctcac gatgacggcc ttcgctatgg ccagtcagga
     1801 cgtccgctac tacctcaacg gcgtactctt cgtcgtcgag ggcaagaagc ttcgcaccgt
     1861 cgccaccgac acccatcgtc tcgcctgctg cgaagtcgac gttgatgacg aaaacacccc
     1921 gatgcaggcc atcatcccgc gcaagaccgt gcgcgagctc attcgcctgc tccccgagga
     1981 cgacacgccc gtccgcgtcc agttcacgga ccttcaggtg tgcttctcct tcggcaacat
     2041 caccttcatg agcaagctca tcgaaggccg cttccccgac tacaagcgcg tgcttccctc
     2101 tcaggacacg aacccgaacg cccttgaact tgagcgcgag gcgatctcca acgcgcttcg
     2161 ccgcgtcggc atcatgacga acgagaagtt ccacggcatc cgctggatcc tcaacggcga
     2221 gggcctgcaa atccagagca cgaactccga ccacgaagag gctctcgaac gcttcgacgt
     2281 gccctggacg cacgacgagc tcgacatggg cttcaacatc acctatctcc ttgaagtgct
     2341 cggcatcctc aagaacaaga ccgtccgctt caacttctcc accgcctccg gcagcgtgct
     2401 gatcacgatg cccgaatcgc aggagcgctt ccgctacgtc gtgatgccga tgcgcatctg
     2461 acgctccgag cgcacgagag gaaaccgaac tagaacaagg ctcggcaagc accgcagcct
     2521 gcataccgct tactgcagac cggctcaggg ccggtctgcc gaaggattta ttcaatgacc
     2581 gaaatggaca cgaacagcga tcagcaaccg accactgcgt ctacctacga cgagcacagc
     2641 atccagattc tggagggcct ggaagccgtt cgcaagcgtc cgggcatgta catcggcgac
     2701 accgccgacg gctccgggct tcatcatctg gtttatgaag ttgtcgacaa ctccatcgac
     2761 gaagctcttg caggctacgc cacgaagatt caggtcacca tccacacgga caactcgatt
     2821 tccgtcgtgg acgacggccg cggcattccc tcgtccatca agtgggacga caagcacaag
     2881 cccaagcgca gcgcagcgga aatcgccctg accgaacttc atgccggcgg caagttcgac
     2941 aacaacggct acaagatctc cggcggcctg cacggcgtcg gcgtctcctg cgtgaacgcg
     3001 ctttcgacct ggctgcgcct catcgtgcgc cgcgacggcg aagcccgtct cctcgaattc
     3061 gaacgcggca aggtcaagaa ccgcctcact gaaacggcca aggatcccgt cacgggcgcc
     3121 gaagtcctca tctctccgat gaagcttctc ggtccgacga gccgtcgcgg cacggaagtg
     3181 cacttcctgc ccgacacgca aatcttcgag cagatcaccg agttcaacta cgacaccctg
     3241 ctctcgcgcc tgcgcgagct ttcgttcctc aactccggcg ttctcatcga gctcaaggac
     3301 cagcgcaccg cacacgaagc ccagcttctg acggacaagg gcctcgtcgc ctacgtccag
     3361 ttcaagaacc agagccgcac ggtgctccac cagaagatct tccacgccaa agaaacggtc
     3421 tacgaaaacg gcatccccat tgaggtcgag atcgccatgc agtggcagga cggctacagc
     3481 gaagtccttt ccgcctatac gaacaacatt ccgcagcgcg acggcggcac gcatgtgacc
     3541 ggcctgcgca tggccatgac ccgcgttctc aagaactaca tctccgccaa cgaaatcggc
     3601 aagaagagcg gcagcaagaa ggccgacatc gatcccgagg gcgacgacat gcgcgaaggc
     3661 ctgacgtgcg tcctctccgt gaaggtgccg cagcccaagt tcagctccca gacgaaggac
     3721 aagctcgtct cgagcgaggt ccgtcccgcc gtcgaggagg tcgtgtcccg cgagctcgag
     3781 ctctacctcg agcaaaatcc cgccgatgcc aagatcatct gcgagaagat cgtggcggcc
     3841 gcccgcgccc gcgaagccgc ccgcaaggcc cgcgacatct cccgcaagag cttctccggc
     3901 ggcggcctgc cgggcaagct cgccgactgc cgtgaaaaga atccggccaa cagcgaactc
     3961 ttcctggtgg agggtgactc cgcaggcggc tcggccaagc agggccgaga ctccaagttc
     4021 caggccattc ttccgctgcg aggcaaggtg ctcaacgtgg aaaaggcctc tcaggacaag
     4081 ctcctcgact ccgagcagat ccgcatgctg acgcttgcgc tcggcaccaa catcggcaag
     4141 gacttcgaca tctcgcgtct gcgctaccac cgcatcatca tcatgaccga tgctgacgtg
     4201 gacggcgcgc acatccgcac gctgctcctg acgctcttct accgccagat gcctcagctc
     4261 atcgagaacg gtcacgtcta catcgcccag ccgcctctct acaaggtgaa gaagaaccag
     4321 gccgacaagg gcgtgttcct caaggacgag cgtgaaatga gcgactatct gctcaagatc
     4381 gccatggagg acgcacgcct ctaccgcaac aaggacgcct tcgacgccga cacgccggtg
     4441 cgcggctccg agctcgagac gcttgtcaac gaataccttc agtcgagcga cgtcatcgcc
     4501 cgccttcagg gcgtgatcga caagtccgtg ctcgtggcca tcgccgcagg cgtcgacatc
     4561 aaccttgaaa acgagttcac ctcgcaccac acggccgaag tgcttcagca ggagcttcgc
     4621 gatccgaacg tcaaggtcga ggccgtctac gacgaggaga acgagaagca cctgctcaag
     4681 gtccaccgtc tcaagcacgg caattacaag acgacgaacg tcacgcccga gttcacgctc
     4741 tccgccgact acaggaagct gcaggaaagc gccgcaatgc tccgcggcat catcggcacg
     4801 ggcgcccgcg tcgcgcgcgg caagaacgac caggccgtca agaacttcgg cgaagccgtc
     4861 aactggctgc tgaagcaggc tgaaaagggc atcatccgcc agcgctacaa ggggctgggt
     4921 gaaatgacgg ccgagcagct caacctgacg accatggatc cgtccgtgcg ccgcatgctg
     4981 cgcgtccgaa tcgaagatgc cgccagcgca gacgccatct tctcgatgct gatgggcgac
     5041 gtcgtcgaac cgcgccgcga cttcatcgag accaacgcgc tctttgccgg caacatcgac
     5101 gcctgatcgc cgagggaaac aggatggccg gcgccgctgg ccttccgctt tcaccgcctc
     5161 aaaccattgg aggacgccgc gtggataatc tcaaggaaaa agtggtcatc gccgtttcct
     5221 caagggcgct gttcgatctt gaggaggagc acgcgctttt cgaggaaaag ggctacaaga
     5281 cattcaggga ccatcaggtc cgctaccaga acacgcccct gcgtccgggc gtggcctttc
     5341 ccttcgtgcg ccgcctgctc gccctcaacg aactctacgg cgatctggcg cccttcaggg
     5401 tcattgcgct ctcgcgcaac tctcctgaga cggcccagcg cttcttccat tcgtgccgcc
     5461 actacaggct gcccatttcg gcgggcggct ttacgtcggg ccactcgacc tatccgtatc
     5521 tggccgcctt caacgcctct ctctttctga gcgccaaccc cgagaatgtc cgcaatgcca
     5581 tcgccgcagg ccagcctgcg ggcctcgtga tgccgtctcc cgtcgg
//
LOCUS       DBMDTI010000124         5590 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3573_length_5590_cov_2.246857, whole genome shotgun sequence.
ACCESSION   DBMDTI010000124 DBMDTI010000000
VERSION     DBMDTI010000124.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3573_length_5590_cov_2.246857"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="ACZPB6_06670"
     CDS             complement(<1..1020)
                     /locus_tag="ACZPB6_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR1184184.1"
                     /translation="MSWDFEKHYINSEWTPSEDDAWIEVENPATLERFARIPDGTPAD
                     ADRAVAAAKVALEGWRTTPLAERIRLMKAMLANFEALHDEIIPLEVAELGAPVGFTTR
                     THCEYQYVRVRSYIEAAERMVLEETLPNSTVYREPVGVVACVTPWNYPLGQVVQKVVP
                     AILMGCTVVLKPSQHTPLTACLLADAFDRAGFPKGVFNMVSGRGSRLGDRLASHPDVD
                     MVSFTGSTTVGIRIAQRALESVKHISLELGGKSPAVFLPSDDYSAAYPKILSSIFFNA
                     GQTCTALSRLIVPRRDLARIESEFCELAAKLVVGDPTDPKTDIGPVSSKAQFEKIAEY
                     VQAGID"
     gene            1493..1738
                     /locus_tag="ACZPB6_06675"
     CDS             1493..1738
                     /locus_tag="ACZPB6_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184185.1"
                     /translation="MTFNAKLLTATAAALIGAIALTGCNEAPAPAPAAEPAAPTQPAP
                     APEAQQQAPEQAQQQAPAEAQKAPEQAQPAPAPDAKK"
     gene            1845..2144
                     /locus_tag="ACZPB6_06680"
     CDS             1845..2144
                     /locus_tag="ACZPB6_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184186.1"
                     /translation="MTNTIKLLAVASVFLASSAFAANPPAAPEPEAQPTVKAAPADLQ
                     APAPVADVQDPALKLPVDLTPADKAQTADEPQPQDGKQAPQPEKAAQPDKAGKAK"
     gene            2225..2674
                     /locus_tag="ACZPB6_06685"
     CDS             2225..2674
                     /locus_tag="ACZPB6_06685"
                     /inference="COORDINATES: protein motif:HMM:NF016087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE"
                     /protein_id="HIR1184187.1"
                     /translation="MKNASRISIAALCAAAALGLAGCNSPSSGTAPEVAPVQVQAQDL
                     TPMQKAAGVYAGELPCADCSGIRTTLYLRANGVYTRISEYVGRDTFEDGGQWTIDEKG
                     VVTMTPAVKGGQPSLARVEEGAVRLLNADGDAVEGPMADLYVLKKDQ"
     gene            2792..3049
                     /locus_tag="ACZPB6_06690"
     CDS             2792..3049
                     /locus_tag="ACZPB6_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184188.1"
                     /translation="MQTSSPRLGPNAAGSRGARDNAHVVTLIQEALCSGDARCVVEAV
                     EKACRLKGLPVPHELYGEMTLESLIAVMAAIGLDLRTERLN"
     gene            3421..3810
                     /locus_tag="ACZPB6_06695"
     CDS             3421..3810
                     /locus_tag="ACZPB6_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184189.1"
                     /translation="MLTRRKLIMGAVATALVGLGATPALARDERLLADILYGVSDALV
                     RDYIRDRYEDGRWDGHYWYYGGHRYTPHEYGRYWAEEYHRQPPKKPKKRKKPHPHPRD
                     WDDDDDDDDDRRRPHHRPPPPPPPHWG"
     gene            4013..4300
                     /locus_tag="ACZPB6_06700"
     CDS             4013..4300
                     /locus_tag="ACZPB6_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184190.1"
                     /translation="MTETADEARRRLVLAALILPVLCAAPVVPALSARAAEPEAPDEE
                     EDDEEPREDQDDEEESEEGPLHDDDADEDSDVNHGFDERAGDRGDDRSAEH"
     gene            4348..4587
                     /locus_tag="ACZPB6_06705"
     CDS             4348..4587
                     /locus_tag="ACZPB6_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184191.1"
                     /translation="MLSRRRIIIAMAALTAASPAASLAARAAASCPRLQAERRAPLQQ
                     DFTHACGRCWDGHHWSWGGHPLHARPGRLLEARES"
     gene            4768..5199
                     /locus_tag="ACZPB6_06710"
     CDS             4768..5199
                     /locus_tag="ACZPB6_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184192.1"
                     /translation="MLNRRKVLFGAAALMAAALPAMPVMASDSRLLADILAGVSDALT
                     RDYIRRHYQEGRWDGSHWNYGGRRYTAAEYGRYWSKHYEERREPKKPSGPAKPPQHPQ
                     HPQQKPGPQAGRNPPKGPGHDNGRHEGRKNDGRRDNWDGPR"
BASE COUNT         1055 a         1808 c         1810 g          917 t
ORIGIN      
        1 gtcgatgccg gcctgcacgt attcggcgat cttctcgaac tgggccttcg aggagacggg
       61 gccgatgtcg gtcttcgggt cggtcgggtc gccgacgacg agcttggcgg cgagctcgca
      121 gaattcggac tcgatgcgcg cgaggtcgcg acggggcacg atgaggcgcg agagagccgt
      181 gcaggtctgg cccgcgttga agaagatcga gctcaggatc ttggggtagg cggccgagta
      241 gtcgtcggag ggcaggaaca cggcggggga cttgccgccg agctcgaggc tgatgtgctt
      301 gacgctctcg agcgcgcgct gcgcgatgcg gatgccgacg gtggtggagc ccgtgaagct
      361 caccatgtcg acgtcgggat gcgaggcgag gcggtcgccg agtctggagc cgcggccgct
      421 caccatgtta aagaccccct ttgggaagcc cgcgcggtcg aaggcgtcgg cgagcaggca
      481 ggccgtgagc ggcgtgtgct ggctgggctt gagaacgacg gtgcagccca tcaggatggc
      541 ggggacgacc ttctggacga cctggccgag cgggtagttc cagggcgtga cgcaggcgac
      601 gacgccgacg ggctcgcggt agacggtgga gttcgggagc gtttcctcga ggaccatgcg
      661 ttccgccgcc tcgatgtagg agcgcacgcg cacgtactgg tactcgcagt gggtcctggt
      721 cgtgaagccg acgggtgcgc cgagttcggc cacttcgagc gggatgatct cgtcgtgcag
      781 ggcctcgaag ttcgcgagca tggccttcat caggcggatg cgttctgcga ggggcgtcgt
      841 gcgccagcct tcgagcgcga cctttgcggc cgcaacggcg cggtccgcgt cggcgggcgt
      901 gccgtcgggg atgcgggcga agcgctcgag cgtggcgggg ttctcgacct cgatccaggc
      961 gtcgtcctcc gaaggcgtcc attcgctgtt gatgtagtgt ttttcaaaat cccatgacat
     1021 tttgcgaatc cttcttcttc tgtttctgtt tttgccggcc tcggggcgcg tcagcatccg
     1081 cgtcggggcc ggaagcaggg caccagatgt cctaactcta gcgcaaagcg aggggctctg
     1141 cgggcctgcc cataatgcta cagcctcagg gaagccgaag agataaggcg ttttgagtag
     1201 gcgcatctct tgtgcgggcg gcggggcggg aggacctccc gcagttgcgc acgccgtgcc
     1261 gctcccggat ttttcggctt atttcctgct gcaacagcct cacgcagtcg gacatgattt
     1321 gttgcgcaaa agcggcacac cgactggcaa gatgtcttca acggacgaac gacgggaacc
     1381 gaagcgaagg aagccccgtc gggcggaccg ccatccgaga aacagcatac atataggaga
     1441 gtgcgcgcag gagcaggacc gacactgcaa gcaactgcac gccgacgaca tcatgacatt
     1501 caacgccaag cttctcaccg ccaccgccgc cgctctcatc ggtgccatcg cgctcacggg
     1561 ctgcaacgaa gccccggctc cggcccccgc cgccgaaccc gccgcgccga cccagcccgc
     1621 tccggccccc gaggcccagc agcaggcgcc cgaacaggct cagcagcagg ctccggccga
     1681 ggcccagaag gcccccgagc aggctcagcc ggcccccgcg cctgatgcca agaagtaagg
     1741 cttcgccttc atgacgggag ggaagatccc tcccgacgct tcgccacgat ccatcaacca
     1801 tatattaagg acggacccgc gccacaacgc agtccgaaca catcatgacg aacaccatca
     1861 agcttctcgc cgtcgcctcc gttttcctcg cttcctccgc ctttgcggcg aacccgcccg
     1921 cggctcccga gcccgaagcc cagccgaccg tcaaggctgc gcccgccgac ctccaggcac
     1981 cggcgcccgt tgccgacgtt caggatcccg cgctcaagct tcccgtcgat ctgacgcctg
     2041 ccgacaaggc gcagacggcc gacgagccgc agccgcagga cggcaagcag gctccgcagc
     2101 ccgaaaaggc cgcccagcct gacaaggccg gcaaggccaa gtaaacacaa gtcaagccga
     2161 caattccaga gagagcggcg cgccgaatcc gacggtgtcg ggtgaaggcg cgccgtcgtc
     2221 acatatgaag aacgcctcca gaatctccat cgccgccctc tgcgcggccg ccgccctcgg
     2281 tctcgcgggt tgcaattcgc cttcttcggg cactgcgccc gaggttgctc cggtccaggt
     2341 tcaggctcag gatctcacgc ccatgcagaa ggccgccggc gtctacgcgg gcgaacttcc
     2401 ctgcgccgac tgctcgggca tccgcacgac gctctacctg agggctaacg gcgtctacac
     2461 gcgcatctcc gaatacgtcg gccgcgacac cttcgaggac ggcggccagt ggacgatcga
     2521 cgagaagggc gtcgtcacga tgacgcccgc cgtcaagggc ggacagcctt cgctcgctag
     2581 ggttgaggag ggtgccgtga ggcttctcaa cgccgacggc gatgccgtcg aggggccgat
     2641 ggcggacctt tatgtcctca aaaaggatca gtgatataaa ccctaaggtc tatggaacgc
     2701 ctcccagagg tcgtgtagac tcatgagcat ggaccattgc ccgatccctc ggacacggtc
     2761 cgttttccca aggacattcc acagagacca tatgcagaca tcgagtcctc gtcttggccc
     2821 caacgctgca ggaagcaggg gcgcacgcga taatgcgcac gtcgtcaccc tgatccagga
     2881 ggcgctctgc tcgggcgacg cacgatgcgt cgtcgaggcc gtcgagaagg cctgccgact
     2941 caagggcctg cccgtgcctc acgagcttta cggcgaaatg acgctcgagt cgctcatcgc
     3001 cgtcatggcc gcgatcgggc ttgatctcag gaccgagaga ctcaactgag agcctccgaa
     3061 gttcctcaac tgcaccgact tcagggccga ccgctctccg cgcgccggcc tttctgttgc
     3121 ttaaagacaa cggattcgcg cacggaccac tgcggcaggg tttccgagca tgatgtcact
     3181 tccgtggatc acggactgtc tcaaaacgcc tcggcgagca ggcgttcgag caagatgccc
     3241 tgagaggcgc ccctgcgggc ggggttcggc tgggagacgc agcaatattc tgcaaagaaa
     3301 gggtgatgtt tctgaacgcc aagtgattcc cgttgtggct agaatgagtc catgagggag
     3361 gagattggtt tctcctgcag gtctcaaggc ttcaaacgca tacgcatcgg gaggtgccgc
     3421 atgcttacac gcagaaagct catcatgggt gccgtcgcaa cggcgctcgt cggtctgggg
     3481 gcgacgcccg cgcttgcgcg cgacgagcgt cttctcgccg acattctcta cggggtttcc
     3541 gacgcgctcg tgcgcgacta catccgcgac cgctacgagg acggccgctg ggacggccac
     3601 tactggtact acggcggaca ccgctacacg ccgcacgagt acggccgcta ctgggctgag
     3661 gaatatcacc gccagccgcc gaagaagccc aagaagcgca agaagccgca tccgcatccc
     3721 cgcgactggg acgatgacga cgatgatgac gacgaccgtc gccgcccgca ccaccgtccg
     3781 ccgcccccgc ctccacctca ctggggatga cgggagccga ggggcccgat cctggcggac
     3841 ggggccttcc ggagacttca agaaggcagc gactccgacg aggagtgcgc tgcctttctc
     3901 ttggccgcct gtgaagcgtg aacttcgtta cggggtccgt cgggctcggg catgcggatc
     3961 gcgcacctga taagatcgac ggacgacaat gccttttctt tcggagacat tcatgaccga
     4021 aaccgccgac gaggcccgca ggcgcctcgt gctcgccgcg ctcatccttc ctgtgctctg
     4081 cgccgcgcca gtcgtccccg ccttgtccgc ccgggcggcg gagcctgaag cgcccgatga
     4141 ggaagaggat gacgaggagc ctcgcgaaga tcaggacgac gaagaggaat ctgaggaggg
     4201 gcccctccat gacgacgacg ccgacgagga cagcgacgtg aaccacgggt tcgacgagag
     4261 ggccggcgat cgcggtgacg accgcagcgc cgagcattga gccgcatctg cggcaggatt
     4321 tgagaaaagc ccttgagagg agttttcatg ctttcgagac gacggatcat catcgccatg
     4381 gcggccctga cggccgcatc ccccgcagct tcccttgccg cacgagcggc ggcttcgtgc
     4441 ccgaggcttc aggctgaacg acgcgcgccc ctgcagcagg acttcacgca cgcctgcggc
     4501 cgctgctggg acgggcacca ctggagctgg ggcggtcatc cgctgcacgc ccgtccggga
     4561 cgtctgcttg aggcgcgcga gtcctgacga gagggcgcag gggagggctt tgcaaacagc
     4621 tcttgtctcc ctaaaacgac tgtgccgcag taaatgtcag tctatatcaa gagtgatgtc
     4681 caattgttgc ggtcggtctt tccctgaacg agtgttcact aaaatggcct cattcgagca
     4741 gcaaccgttg atgacaggcg gccgcagatg ctcaacagaa gaaaggttct tttcggcgcc
     4801 gccgccctga tggccgcggc ccttcccgcg atgcccgtca tggcgtccga cagcaggctt
     4861 ctcgccgaca tcctcgcggg cgtctccgac gccctgacgc gggactacat ccgcaggcat
     4921 tatcaggaag gccgctggga cggcagccac tggaactacg gcggccgccg ctacacggcc
     4981 gccgagtacg gccgctactg gtcgaagcac tacgaggagc gccgcgagcc gaagaagccg
     5041 tccgggcccg cgaagccgcc gcagcatccg cagcatccgc agcagaagcc cgggcctcag
     5101 gccggccgga atcctccgaa gggaccgggg cacgacaacg gcaggcacga agggcgcaaa
     5161 aacgacggga ggcgcgacaa ctgggacggc ccccgctgac tgaacgctgc atgatgcctt
     5221 gatgagcggt cgaaaacgga gaagtgcaag ggtgtcttta cccttctcca gaagggcgga
     5281 gcgtgagctt cgcccttttt tcgcctgcgg gagtaggaaa gacattggaa accccggctt
     5341 catccgaagg ggcgggcggc gtctgcgtca agaagcttca cgaaatgtac gagacgtgga
     5401 atcctgaacg agcgttcagt aaaataaggt catcaggttc caacaatgat tccgtcttcg
     5461 tgagggaaac accatgctga caagaagaaa gcttgtgctg ggcgccgcgc ttgcggtcct
     5521 cgtgcctgct gcgccggcgc ttgccgcgcc ggatccgctc ctcgccgaca ttctctacgg
     5581 cgtctccgac
//
LOCUS       DBMDTI010000125         5579 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3585_length_5579_cov_2.159912, whole genome shotgun sequence.
ACCESSION   DBMDTI010000125 DBMDTI010000000
VERSION     DBMDTI010000125.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3585_length_5579_cov_2.159912"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="ACZPB6_06715"
     CDS             <1..554
                     /locus_tag="ACZPB6_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1184193.1"
                     /translation="FMDEHAGRKIKSDALFKVIGTDENYPIAVCDDSIVKAINPSFVK
                     LPLEMGTVKKFDTLEALADHFGIKKDAFLEEVKKFNGFVKAGEDKDFGRILSFNNGLT
                     VSQGPFYGIECCPKIHHTMGGVMINEKAEVISATTQKPIKGLYAGGEVTGGVHGASRL
                     GTVAVIDALTFGMIAGENFAAMK"
     gene            836..2425
                     /gene="nikA"
                     /locus_tag="ACZPB6_06720"
     CDS             836..2425
                     /gene="nikA"
                     /locus_tag="ACZPB6_06720"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1184194.1"
                     /translation="MKRTLALAVACALVLEGCGESDAPGEARKKTVLEYASMKDIRNI
                     NPHLYLGEMAAQAMVFEPLVFNTPEGVKPALAERWEISGDGRTYTFHLRRGVKFTDGT
                     PFTADAVKKNIDAVMANRTRHAWLDMINEIESSEVVDEHTWRLHLRHPYFPALIELGL
                     SRPFRFISPACMKDGGTKDGVTCLAGTGPWVLSEHKDQQYALFSRNEGYWGDKPIVPT
                     VRWNVVPDTQTMLMALEKGEIDLVFGADGDQLTGDAFKKLTDEKRLNTAVSEPIASRA
                     ILVNSKRPITKDVRVREALAYAFNRQAVVDGILNGTEAAAMTLFAKSVPYCDVPLKER
                     GHDPKKAAEILDAAGWKAGADGIRTKDGARLVLDFYFNSNNAQEKTIAEAMQGDLLKI
                     GVELRVIGEEKQAFLDRQRTGEFDLQYSLSWGAPYDPQSYLSSWRIPAHGDYQSQIGL
                     ADKAHIDATITALMTEADEKKRAAMVRDVLTRITDAAVYIPVSYSRTKAVYSNRIKDV
                     SFAVTQYEIPFEKMSFTEAGK"
     gene            2426..3370
                     /gene="opp1B"
                     /locus_tag="ACZPB6_06725"
     CDS             2426..3370
                     /gene="opp1B"
                     /locus_tag="ACZPB6_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt ABC transporter permease"
                     /protein_id="HIR1184195.1"
                     /translation="MGGMLVRRLALLVPMLLGITFIAFMLGELSPSDPAEVSIRVNAM
                     VPTPELIAQTRHELGLDRPLMTRYFEWLAGVLQGDLGTSFVTGHKVTDDFLRAFPPTL
                     ALAGATLLIVLVTGGVCGWLCARHAGGPVDRAIRSVLFTISALPNFWAALLLMWLFSL
                     TLGWLPTNGMRTPESVILPAVSLSLAYVGTYVRLIRTEMLRTAESDWVMFARARGLSE
                     GRISLHMMLNSLRGTVTALGMSIPKLVAGTFVIENIFAWPGIGRLCVSAIFNRDLPVI
                     IAYVLVMAVFFILFNLLSDLALAALDPRARRGGAGEAA"
     gene            3367..4185
                     /locus_tag="ACZPB6_06730"
     CDS             3367..4185
                     /locus_tag="ACZPB6_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1184196.1"
                     /translation="MTNRWRRALAAPSGLFLLAVLAAGLAAPLLPLADPTAIDISSKF
                     LPPSLEHPFGTDLLGRDMLSRMVWGIRTTFVTALLTMIVTAGVGMIAGGAAAAARGRV
                     DALIMRFCDVWMSFPSEVLILALVGMMGPGLENVLIACLLAKWPWYARMMRGLILHLN
                     SAGFVQFARVTGASNMKVLFGHLVPNAAGECFVLGTIDTGSVVLMISALSFLGLGAQP
                     PMPEWGSMLADAKNVMTLYPGQMFVPGLAILLVVAALNFLGDALRDAFDVRRTE"
     gene            4182..>5579
                     /locus_tag="ACZPB6_06735"
     CDS             4182..>5579
                     /locus_tag="ACZPB6_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1184197.1"
                     /translation="MTDADDVILSVAGLRVRDSWDREILHGLDLDVRRGECLALVGES
                     GAGKSLTLRALAGLLPHGFTVSGTMDFEGRTLCAPGAFAAVRGRRILYMAQQAMTAFD
                     PLVRIGVQLGETASAAGLSGSKADEAVSRALRAVGLDPARILRAFPCELSGGMLQRVM
                     TACVLILRPDVILADEPTSALDVVNVRAVLASLQEVRRTTGSALVLVTHDLGLAAGLA
                     DRFVILMSGGIVEKGGREIFERPSHPYTQRLALAWRRANAVLQESLAAGPAPAAEEPQ
                     RAPEAEPVLRIEGVTKYYASGSFLRRSVHEVLRGVNLEVRRGEVVGLIGGSGEGKSTL
                     SRLILGLEAPTAGRILVRGADRSELCSGEASVVFQNYLDSADPVWTVGDVIEEPVRLA
                     GGRVDVRDLLRRVGLPAEYASRRPHQLSGGELQRVAIARAMAGGPSLVVFDEALSSLD
                     ASVQDEIMELLAELKP"
BASE COUNT          926 a         1877 c         1765 g         1011 t
ORIGIN      
        1 gcttcatgga cgaacacgcc ggccgcaaga tcaagtccga cgccctcttc aaggtcatcg
       61 gcacggacga aaactacccg atcgccgtct gcgacgactc catcgtgaag gccatcaacc
      121 cgtcctttgt caagctcccg cttgaaatgg gcacggtcaa gaagttcgac acgctcgaag
      181 ccctcgccga ccacttcggc atcaagaagg acgccttcct cgaagaagtc aagaagttca
      241 acggcttcgt caaggccggc gaagacaagg acttcggccg catcctctcc ttcaacaacg
      301 gtctgaccgt ctcccagggc ccgttctacg gcatcgaatg ctgcccgaag atccaccaca
      361 cgatgggcgg cgtcatgatc aacgaaaagg ccgaggtcat ctccgccacg acgcagaagc
      421 cgatcaaggg cctctacgcc ggcggtgaag tgaccggcgg cgtccacggc gcctcccgtc
      481 tcggcacggt ggccgtcatc gacgccctga ccttcggcat gatcgctggc gaaaacttcg
      541 ccgctatgaa gtaatcctcc tgcgtccatc ccctcggaca ggatgaccga ccgccaagag
      601 ccggcacaag aggaatacag ggaaaaagga gcacgcctta gggaccgtgc tccttttttc
      661 gtttcaggac aatgacggga tcatgccggc ttcgcgaaac cgatccttat gcacgcctct
      721 gctagttctg ttggcggatg tatgaagatt ctcaaaatag tatgatgtcg ggacggggac
      781 gggctctgag ggccgcgggc ccctccattc actccttacg gaaggacaac atgacatgaa
      841 gcgaacgctt gctcttgccg tcgcctgcgc actcgtgctt gagggctgcg gagaatccga
      901 cgcgcccggg gaagcccgga agaagaccgt gctcgaatat gcgagcatga aggacatccg
      961 caacatcaat ccgcatcttt atctgggcga gatggccgct caggccatgg tcttcgagcc
     1021 gctcgtcttc aatacgcccg aaggggttaa gcccgcgctt gccgagcgct gggagatctc
     1081 cggggacggc cgcacctaca ccttccatct gcgccgcggc gtcaagttca cggacggcac
     1141 gccgtttacg gccgacgccg tcaagaagaa catcgatgcc gtcatggcca accgcacgcg
     1201 ccacgcctgg ctcgacatga tcaacgagat cgagtcgagc gaagtggtcg acgagcacac
     1261 ctggcgcctg catctcaggc atccgtactt ccccgcactc atcgagctcg ggctctcgcg
     1321 ccctttccgc ttcatctcgc ccgcctgcat gaaggacggc gggacgaagg acggcgtgac
     1381 ctgtctcgca ggcacgggtc cctgggtgct ctccgaacat aaggaccagc agtacgcgct
     1441 cttcagccgc aacgagggct actggggtga taagccgatc gtacccaccg ttcgctggaa
     1501 cgtcgtgccc gacacgcaga ccatgctgat ggcgcttgaa aagggcgaga tcgacctcgt
     1561 cttcggcgcc gacggcgacc agctcacggg cgacgcgttc aagaagctca cggacgagaa
     1621 gcgcctcaac acggcggtga gcgagccgat cgcctcccgc gccattctcg tcaactcgaa
     1681 gcgcccgatc acgaaggacg tgcgcgtgcg cgaggcgctg gcctacgcct tcaaccgtca
     1741 ggccgtggtc gacggcattc tcaatggcac ggaggcggcc gccatgacgc tctttgcgaa
     1801 gagcgtgccc tactgcgacg ttccgctcaa ggagcgcggc catgatccga agaaggccgc
     1861 cgagattctt gatgcggcgg gctggaaggc cggtgccgac ggcatccgca cgaaggacgg
     1921 cgcaaggctc gtgctcgact tctacttcaa ctccaacaac gcccaggaaa agacgatcgc
     1981 cgaggccatg cagggcgacc tgctcaagat cggcgtcgag cttcgcgtga tcggcgagga
     2041 gaagcaggcc ttcctcgacc gccagcgcac gggcgaattc gacctgcagt actcgctctc
     2101 ctggggcgcg ccgtacgacc cgcagagcta tctctcctcc tggcgcattc ccgcgcacgg
     2161 cgactatcag tcccagatcg ggcttgccga caaggcgcac atcgacgcga ccatcacggc
     2221 cctcatgacc gaggccgacg agaagaagcg cgccgctatg gttcgcgacg tgctcacgcg
     2281 catcacggat gcggcggtct acatccccgt ctcctattcg cgcacgaagg ccgtttattc
     2341 gaacaggatc aaggacgtct cctttgcggt gacgcagtac gagatcccgt tcgagaagat
     2401 gagcttcacg gaggcgggca aataaatggg cggcatgctc gtgcggcgcc tcgcgctcct
     2461 cgtgccgatg cttctgggca tcaccttcat cgcgttcatg ctgggtgaac tctcgccctc
     2521 tgatcccgcc gaggtgtcga ttcgcgtgaa cgcgatggtg cccacgcccg agctcatcgc
     2581 ccagacgcgt catgaactcg ggctcgaccg ccctctcatg acgcgctact tcgagtggct
     2641 cgcgggcgtg ctgcagggtg acctcggcac gagcttcgtg acggggcaca aggtgacgga
     2701 cgacttcctg cgagccttcc cgcccacgct ggcgctcgcg ggcgcgaccc ttctgatcgt
     2761 gctcgtgacg ggcggggtgt gcgggtggct ctgcgcaagg catgcgggcg gtcccgtcga
     2821 cagggcgatc cgcagcgtcc tcttcacgat ctcggcgctg cccaacttct gggccgcgct
     2881 ccttctgatg tggctcttct cgctcacgct cgggtggctt ccgacgaacg gcatgaggac
     2941 tcccgagtcg gtgattctgc ccgccgtctc gctctcgctc gcctacgtcg gcacttacgt
     3001 tcgcctcatc cgcaccgaaa tgctgaggac ggccgagtcc gactgggtga tgttcgccag
     3061 ggcgcgcgga ctctccgagg ggcggatctc gcttcacatg atgctcaaca gcctcagggg
     3121 taccgtcacc gcactcggca tgtcgattcc gaagctcgtc gcgggcacct tcgtgatcga
     3181 gaacatcttc gcctggcccg gcatcggcag gctctgcgtc tccgccatct tcaacaggga
     3241 tctgcccgtg atcatcgcct acgtgctcgt gatggccgtg ttcttcatcc tcttcaatct
     3301 gctttcggac cttgcgctgg cagcgcttga tccgcgcgcc cgcagaggcg gcgcagggga
     3361 ggcggcatga cgaaccgctg gcgcagggcg ctggccgccc cgtcgggcct cttccttctc
     3421 gccgtgctcg ctgcaggcct tgcggcgccc ctgctgccgc tcgccgatcc gacggccatc
     3481 gacatttcgt cgaagttcct gccgccgagc ctcgagcatc ccttcggcac cgatcttctc
     3541 ggccgcgaca tgctctcgcg catggtctgg ggcatccgca ccaccttcgt gacggcgctc
     3601 ctcacgatga tcgtgacggc gggcgtcggc atgatcgctg gcggcgctgc ggcagcggcc
     3661 cgaggcaggg tcgatgcgct catcatgcgc ttttgcgacg tgtggatgtc gttcccgagc
     3721 gaagtgctca tcctggccct cgtcggcatg atgggcccgg ggcttgagaa cgtgctcatc
     3781 gcctgcctgc tcgccaagtg gccttggtac gcacgcatga tgcgcggcct catccttcac
     3841 ctcaattcgg ccggcttcgt gcagttcgcg cgtgtgaccg gcgcctccaa catgaaggtg
     3901 ctcttcggac acctcgtgcc caatgcggcg ggcgaatgct tcgtgctcgg cacgatcgac
     3961 acgggcagcg tcgtcctcat gatctcggcg ctctccttcc ttggcctcgg cgcacagccg
     4021 cccatgcccg agtggggctc catgctcgcg gacgcgaaga acgtgatgac gctttatccg
     4081 ggacagatgt tcgtgcccgg cctcgcgatc ctgctcgtgg tggcggcgct caacttcctc
     4141 ggcgacgcgc ttcgcgacgc cttcgacgtg aggaggaccg aatgacggac gcagacgacg
     4201 tgattctcag tgtcgcgggc cttcgcgtcc gtgattcctg ggacagggaa atcctgcacg
     4261 ggctcgacct cgacgtgcgc aggggcgagt gccttgcgct cgtgggcgag tcgggcgcag
     4321 ggaagtccct gaccctgagg gcgcttgcgg ggcttcttcc gcacggcttc acggtctcgg
     4381 ggacgatgga cttcgagggc cggacgctct gcgcgccggg cgccttcgcc gcagtgcgcg
     4441 gcaggcgcat cctctacatg gcccagcagg cgatgaccgc ctttgatccc ctcgtgagga
     4501 tcggcgtgca gctcggcgaa accgcttcgg ccgcaggact ttcgggctcg aaggccgatg
     4561 aggccgtgag cagagcgctt cgtgcggtcg ggctcgatcc cgcccgcatc ctcagggcct
     4621 tcccgtgcga gctttcgggc gggatgctcc aacgcgtcat gaccgcctgc gtcctcatcc
     4681 tcaggcccga cgtgattctc gccgacgaac ccacgagcgc gcttgacgtc gtcaacgtca
     4741 gggccgtcct cgcgagcctt caggaggtga ggaggacgac gggctcggcg ctcgtgctcg
     4801 tcacgcacga cctcggcctc gcggcgggcc ttgcggaccg cttcgtgatc ctcatgtcgg
     4861 gcggaatcgt cgaaaagggc ggccgcgaga tctttgagcg cccctcgcat ccctatacgc
     4921 agaggcttgc gctcgcctgg cggcgtgcga acgccgttct gcaggaatcc ctggctgcag
     4981 gccctgcgcc cgcagcggag gagccgcagc gtgcgccgga agctgagccc gtgctccgga
     5041 tcgagggcgt cacgaagtac tatgcctctg gatccttcct gcggcggagc gtgcacgagg
     5101 tgctccgcgg cgtgaacctt gaggtccgca ggggcgaggt cgtcggactc atcggcggct
     5161 cgggcgaggg aaagtcgacg ctctcgcgcc tcatcctcgg gctcgaggcg ccgacggcgg
     5221 gccgcattct cgtgaggggc gctgaccgct ccgagctttg ttcgggcgag gcgagcgtcg
     5281 tcttccagaa ctatcttgac tcggccgatc ccgtctggac ggtcggggac gtgatcgagg
     5341 agcccgtgag gcttgcgggc ggccgcgtcg atgtgcggga ccttctcagg cgcgtgggac
     5401 ttcccgccga gtacgcgtcg cgccgtcccc accagctctc gggcggagag ctccagcgcg
     5461 tcgcgatcgc gcgcgccatg gcgggcggcc cgtcgctcgt cgtcttcgac gaggcgctca
     5521 gcagcctcga cgcctccgtg caggacgaga tcatggagct tcttgccgag ctcaagccc
//
LOCUS       DBMDTI010000126         5545 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3605_length_5545_cov_2.112417, whole genome shotgun sequence.
ACCESSION   DBMDTI010000126 DBMDTI010000000
VERSION     DBMDTI010000126.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3605_length_5545_cov_2.112417"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="ACZPB6_06740"
     CDS             complement(<1..344)
                     /locus_tag="ACZPB6_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR1184198.1"
                     /translation="MLTKKALGLMIGALLSGTLIGGEAAAGQTLADFHSKMGGCASCH
                     GTNAVTAASVPDDERALNAQCQTCHGSYKDIRKTGTEIDPHHSHLGDINCTSCHTAHA
                     RPKLVCNDCHTFP"
     gene            complement(721..1341)
                     /locus_tag="ACZPB6_06745"
     CDS             complement(721..1341)
                     /locus_tag="ACZPB6_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR1184199.1"
                     /translation="MVRKSWLEKGHRAGGEGRESDPFVRVSWEKAAKLVAGELKRVRE
                     TYDPGAIWGGSYGWMRTSSVGNARNLLQRVLNLNGGYTTYTGDYSTGCAQVVLPYVIG
                     SNGVYEQVTSRELINEKTELVVLWGADPTITNDIDWCTTIHENAGGLRALKARGVKVV
                     AINPLKPDTAECFGDKAQGIAPRPGTDVAMMLGRTSRACASGSSPK"
     gene            complement(1319..1564)
                     /locus_tag="ACZPB6_06750"
     CDS             complement(1319..1564)
                     /locus_tag="ACZPB6_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184200.1"
                     /translation="MTNRRNLFRAAGALAGAAFIPAAHAASPKHPSGMNGVRTLKGGR
                     IRALQARPRAVAPAAGLCPAALQPRPHPPSDGSQELA"
     gene            complement(1649..1999)
                     /locus_tag="ACZPB6_06755"
     CDS             complement(1649..1999)
                     /locus_tag="ACZPB6_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184201.1"
                     /translation="MHDAGLRIQYAAFDEDGAVKLSGAEKSVQGNAWQAASAEGWVPP
                     AGLTLDLDGLWKKAEVRHADACSTCHGAPKASAHTANEWAGQLPKRGGRTGHSSAGAN
                     ELMFKWLQAHAKPM"
     gene            complement(2282..3571)
                     /locus_tag="ACZPB6_06760"
     CDS             complement(2282..3571)
                     /locus_tag="ACZPB6_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1184202.1"
                     /translation="MDKAAIKQKVCEAIAAHRADIEAISEAILAEPELGFKEVKTSRK
                     VQEALERLGIEFTAGHGITGVKARMKGRNSKRTVAMLAELDAIVCRNHRNADPVTGAA
                     HCCGHNIQIANMIAVACGLRDVMSELDGDVVLFAVPAEEYVEIDWRNAQRDEGRLKYL
                     GGKQQLIAEGAFEDIDMAMQMHVASGDEFNLGASSNGFIGKLIEYHGRPAHAAFAPEE
                     GINALNAAMMGVMGVNAIRETFRDQDHVRFHPIINAGGDLVNVIPDYVKMESYVRASN
                     IDAMARYNKAVDRALRAGGDAIGAKCEIRNLPGYLPLAPDDAFREVLRDNAVELFGKE
                     KVTLGEHSSGSTDMGDVSLLMPVIHPWVGCVDGVLHGADYELRDREAAFTRIPQALAL
                     TIVDLLADGAQKADEICTAFKPRLDRESYVAFMDDIR"
     gene            complement(3721..4194)
                     /locus_tag="ACZPB6_06765"
     CDS             complement(3721..4194)
                     /locus_tag="ACZPB6_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184203.1"
                     /translation="MNAIAAHLEKLKDYIFILLVTGLYTVVANTFGYNGDLAQGAVGI
                     LVIVAIAFFGVVIQMLPGFNKLPVVFWVSLTAVIVSIPSFPGGVWIVEQTKHINFLAT
                     TTPILAYGGLCLGKDLEAFKRLSWRIVPVAMAVASGSFLCATALAELMLHLEGIF"
     gene            complement(4243..5016)
                     /locus_tag="ACZPB6_06770"
     CDS             complement(4243..5016)
                     /locus_tag="ACZPB6_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HIR1184204.1"
                     /translation="MDLIKNWKLHLLALIVVIVAESIGMHRFGLVVLLPLLYALVLGG
                     ILSFPKFGILKFSEMDRAAKFMPIAMLVLLTKIGLGIGPNLEMLLNSGWALIMQEFGH
                     FFGTLIFGLPVALLLGMKREAIGACYSIDREPNVAIIGEKYGLDSPEGRGVMGMYICG
                     TVFGALWISILAGVVAGLDIFHPHSLAMGAGIGSASMMAAGTGSIIAAYPEAAETVGA
                     YAAAANLMTSVLGIYFALFISLPVTVKIYELVTGDKRAA"
     gene            complement(5253..5387)
                     /locus_tag="ACZPB6_06775"
     CDS             complement(5253..5387)
                     /locus_tag="ACZPB6_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184205.1"
                     /translation="MPECRIEALGLARQPLNEIEAMLALTSVTSASSTSSSTSMRGSL
                     "
BASE COUNT          915 a         1542 c         2031 g         1057 t
ORIGIN      
        1 gggaaggtgt ggcagtcgtt gcagacgagc ttcggacggg cgtgcgcggt gtggcagctc
       61 gtgcagttga tgtcgccgag atgcgagtgg tgcggatcga tttccgtgcc cgtcttcctg
      121 atgtccttgt aggagccgtg gcaggtctgg cactgcgcgt tgagcgcacg ctcgtcgtcg
      181 gggacggacg ctgccgtcac ggcgttcgtg ccgtggcacg aggcgcagcc gcccatcttc
      241 gagtgaaagt ccgcgagggt ctggcccgcc gccgcttcgc cgccgatgag ggtgccgctc
      301 agaagcgcgc cgatcatcag gccgagtgcc ttcttcgtca acatgatgtc ttctcctttt
      361 gagtgttgtt gtgacgagag gcggttcatg ccgccttccg atgaatcgag tctaggcgcg
      421 ggggaggtca aaatcttgat ttgcgtcaag tcgaaaaccg aaggcgccgc aaagttctgc
      481 aggagtatta agaaaaactg gaaatgcttg ggttttcgag tatgtcgatt caggtttttt
      541 gcggggtgcg gggcgaagag tgcggcgcag aggtgcgtgc gggaagaggg cggaatgtga
      601 gggcgaaaga caagagaggg cccgcagacg caagcctgcg gaccctctcg ggatccgtcg
      661 ggatcagccg gccgcgggtt caggaccggg ccggtctcgg acgtcagtcg tcctcacgca
      721 ctatttcggg ctgctcccag acgcgcatgc gcgggaggtt ctgccgagca tcatcgcgac
      781 gtcggtgccg ggacgcggcg cgatcccctg cgccttgtcg ccgaagcatt cggccgtgtc
      841 gggcttgagc gggttgatcg ccacgacctt gacgccgcgg gccttaaggg ctcgcaggcc
      901 gcccgcgttc tcatgaatcg tcgtgcacca gtcgatgtcg ttcgtgatcg tcggatcggc
      961 gccccagagg acgacgagtt cggtcttctc gttgatgagc tcccgggacg tcacctgttc
     1021 gtagacgccg ttggagccga tgacgtaggg caacaccact tgggcgcagc ccgtggagta
     1081 gtcgcccgta taggtcgtgt agccgccgtt gaggttgagg acgcgctgca ggaggtttcg
     1141 ggcgttgccc acggagctcg ttctcatcca gccgtaggaa ccgccccaga tggcgccggg
     1201 atcgtaggtc tcgcgaacgc gcttgagttc gcccgcgacg agcttcgcgg ccttttccca
     1261 agagactcgc acgaacggat cggactcgcg gccttcgccg cctgcgcggt ggcccttttc
     1321 aagccagctc ttgcgaacca tcggatggcg gatgcgggcg tggttgtagg gctgctgggc
     1381 aaaggcctgc agccggagcg acggcgcgag gtcgcgcttg aagggctctg atgcggccgc
     1441 ccttgagcgt gcgcacgccg ttcatgccgg acgggtgctt gggagaggcc gcatgagcgg
     1501 cggggatgaa ggcggcgccg gcaagcgcgc cggctgcgcg gaagagattt cggcgattgg
     1561 tcatgtcgct ttttccttgt tcacggttgg tcggagtgtg aatgcctgtt gtcccgaagg
     1621 ctgttcggaa gcggacggcg gggacgtgtt acatgggctt ggcgtgcgcc tgcagccact
     1681 tgaacatcag ttcgttggcg cccgcgctcg agtggccggt gcggccgccg cgcttgggca
     1741 gctggcccgc ccattcgttg gcggtgtggg cggaggcttt gggcgcgccg tggcaggtgg
     1801 agcaggcgtc ggcgtgacgc acctcggcct tcttccagag tccgtcgaga tccagagtga
     1861 gcccggcggg gggcacccag ccttctgcgc tcgcggcctg ccaggcgttg ccctgaacgg
     1921 acttttcggc gcccgaaagc ttcacggcgc cgtcctcgtc gaaggcggcg tactggatgc
     1981 gaaggccggc atcgtgcaaa tcactcgccg cgtgtgcgct cggcacagtc ctcgcctcct
     2041 cagcgctttt gtaacggttt tgcgagcgtt tggaatgaga gcgaagtggt atccatcccg
     2101 tgagtggcgg gtgttcggag gagggcggga ggggaagtcg gcttgcggga gagggaaaag
     2161 gaggggcgtg atggggagat gaggaaaaaa aggggacggc gctctgctgc aacgccgtcc
     2221 cgaaaccccc gcggcctttg ccggaccgca tgggacagga aatcaatgtg tggatgaggc
     2281 cttatctgat gtcgtccatg aaggccacgt aggactctcg gtcaagccgg ggcttgaagg
     2341 ccgtgcagat ttcgtcggcc ttctgcgcgc cgtctgcgag caggtcgacg atcgtgaggg
     2401 ccagggcctg cgggatgcgg gtgaaggcgg cctcgcggtc gcgaagctcg tagtcggcgc
     2461 cgtggagaac gccgtcgacg cagccgaccc aggggtggat gacgggcatg aggagcgaga
     2521 cgtcgcccat gtcggtcgag cccgagctgt gttcgccgag cgtgaccttc tccttgccga
     2581 agagttcgac ggcgttgtca cgcaggactt cgcggaaggc gtcgtcgggg gcgagcggga
     2641 ggtagcccgg caggttcctg atctcgcact tcgcgccgat ggcgtcgccg cccgccctga
     2701 gggcgcggtc gacggccttg ttgtagcggg ccatggcgtc gatgttggag gcgcgcacgt
     2761 agctttccat cttgacgtag tcggggatca cgttgacgag gtcgccgccc gcgttgatga
     2821 tcgggtggaa gcgcacgtgg tcctggtcgc ggaaggtttc gcggatggcg ttgacgccca
     2881 tgacgcccat catggcggcg ttgagggcgt tgatgccttc ttcaggtgca aaggcggcgt
     2941 gagcgggacg gccgtggtat tcgatgagct tgccgatgaa gccgttgctc gaggcgccga
     3001 gattgaattc gtcgcccgag gccacgtgca tctgcatggc catgtcgatg tcctcgaagg
     3061 cgccttcggc gatgagctgc tgcttgccgc cgaggtactt gagacgaccc tcgtcgcgct
     3121 gggcgttgcg ccagtcgatc tcgacgtact cttcggcggg gaccgcgaag aggacgacgt
     3181 cgccgtcgag ttcggacatg acgtcgcgca ggccgcaggc cacggcgatc atgttggcga
     3241 tctggatgtt gtggccgcag cagtgggcgg cgcccgtgac ggggtcggcg ttcctgtggt
     3301 tgcggcagac gatggcgtcg agctcggcga gcatcgcgac ggtgcgcttc gagttcctgc
     3361 ccttcatgcg ggccttgacg cccgtgatgc cgtggccggc cgtgaattcg atgccgagcc
     3421 tctcgagcgc ctcctgcacc ttgcgggagg tcttcacctc cttgaagccg agttcgggct
     3481 cggcgaggat ggcttcggaa atcgcctcga tgtcggcgcg gtgcgcggcg atggcttcgc
     3541 agactttctg cttgatggcg gctttgtcca tgatctgtac aggggaccgt ttccggcgcc
     3601 tgatggcggc gcctgcggtc cgctcctttc tatatctggt gattgacggg accgggtgcg
     3661 cccgcccccg caggggcggc gggcattcgg tccattgtag aacgattgtc ggaacgagga
     3721 tcagaagatg ccctcgaggt gcagcatcag ctcggcgagc gcggtggcgc agaggaagct
     3781 gcccgaggcg acggccatgg cgacggggac gatgcgccag gagagtcgct tgaaggcctc
     3841 gaggtccttg cccaggcaca ggccgccgta ggcgaggatc ggggtcgtcg tggcgaggaa
     3901 gttgatgtgc ttggtctgct cgacgatcca cacgccgccg gggaagctcg ggatcgagac
     3961 gatgacggcg gtgagcgaga cccagaagac gacggggagc ttgttgaagc cgggcagcat
     4021 ctggatcacg acgccgaaga aggcgatcgc gacgatgacg aggatgccca cggcgccttg
     4081 ggcgaggtcg ccgttgtagc cgaaggtgtt ggcgacgacg gtgtagaggc cggtgacgag
     4141 caggatgaag atgtagtcct tgagcttttc aagatgggcg gcaatggcgt tcatgatgta
     4201 ctttccttgt gacggggggg cggtgcggtg tgttcaggtc tctcaggcgg cgcgcttgtc
     4261 gccggttacg agttcgtaga tcttgaccgt gacgggcagc gagatgaaga gcgcgaagta
     4321 gatgccgagt acagaggtca tcaggttggc ggcggccgcg taggcgccga cggtttcggc
     4381 ggcttcgggg taggcggcga tgatcgagcc cgtgccggcg gccatcatcg aggccgagcc
     4441 gatgcccgcg cccatggcga gggagtgcgg atggaagatg tcgaggcccg cgaccacgcc
     4501 cgcgaggatc gagatccaga gcgcgccgaa gacggtgccg cagatgtaca tgcccatcac
     4561 gccgcggccc tcggggctgt caaggccgta cttctcgccg atgatggcga cgttgggctc
     4621 gcggtcgatc gagtagcacg cgccgatggc ctcgcgtttc atgccgagga gcaacgcgac
     4681 gggcaggccg aagatcagcg tgccgaagaa gtggccgaac tcctgcatga tcagggccca
     4741 gccggaattg agaagcatct cgaggttcgg cccgatgccc aggccgatct tcgtcaggag
     4801 gaccagcatg gcgatcggca tgaacttcgc ggcgcggtcc atttcggaga acttcaggat
     4861 cccgaacttc gggaaggaga ggatgccgcc cagcaccagc gcgtagagca gcggcagcag
     4921 cacgacgagg ccgaagcggt gcatgccgat cgattcggcc acgatcacga cgatgagggc
     4981 cagcagatga agcttccagt tcttgatgag atccatgttg agtctcctag tttttcctgt
     5041 ggggcgaaag gccccgtcca tgggtgcgga cggtcttccg gcggggtttc ccggctcttt
     5101 tgacggattt cgggtgcagt ggggccttga gccttcagtg cgccgaaatg caaaatcggc
     5161 cggtcggatt gtcaaaggga gtgttgggga aagggcggcg gacgttcacg gccggggagg
     5221 ccgcaacggc gcctgatatc gcacgcgcgg gcttagaggg agccgcgcat ggatgtagag
     5281 gaggatgtgg aggaggcgga ggtgacggac gtgagcgcaa gcatggcttc aatctcgttc
     5341 aatggctgcc ttgcgaggcc cagcgcctca attctgcatt ccggcatttt cctgctccag
     5401 tggtgttctt caatgcccgc cgcattcgtc tgcaggggcg tgacagaaaa taccctagca
     5461 gacctgcaac aaatgttctc aaattcaaac acggagtttg acgaaagtca aggcttcttg
     5521 ggcggaagta cttggatttc acgtg
//
LOCUS       DBMDTI010000127         5450 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3671_length_5450_cov_2.345111, whole genome shotgun sequence.
ACCESSION   DBMDTI010000127 DBMDTI010000000
VERSION     DBMDTI010000127.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3671_length_5450_cov_2.345111"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(101..460)
                     /locus_tag="ACZPB6_06780"
     CDS             complement(101..460)
                     /locus_tag="ACZPB6_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184206.1"
                     /translation="MDRSMIRKSMLLVMTAAVAACIAGCSKEDIEAAREAGTNAVSKV
                     ADKTIELSDEAKGVVDRTKEHIHERIVKMPEETTRQAAEILGAAGEGAKDASELLNEH
                     AVSLQEGLEFRSKDREK"
     gene            complement(605..1798)
                     /locus_tag="ACZPB6_06785"
     CDS             complement(605..1798)
                     /locus_tag="ACZPB6_06785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1184207.1"
                     /translation="MFVRSGILAASILLSVTSCAAPSLEDAQRHVMPLGQTSFAQYAA
                     DAKAWIGANRSFVLDTDEGRKMELEANLPKEYRPEHSDGRGILLVHGLGDSPWSFSDI
                     AQSLAANGILVRTVLLPGCGTQPADMMPATADDWRRVVQEQADILSREVDEMWLGGYS
                     TGCNLVLDYADANPGRTKGFVLFSPAVEVRPPFAWAASFVSNFRDWLVTPESRPNGGR
                     NPFQYFVVPMKGFAAFHDTMVRADDVLERLEKKPWSGPAAVMLAEHDGLVETEDLLAR
                     FDRAFPNPRTRIFWYGEERFARGLSAHVRILPEAVPEKRIASFSHMSMTYRPDNPMYG
                     ESGAERLCWNGQSKAESKACEEGAEIWYSGERHQPGDGRNCAKLTFNPWYDEQLAGIL
                     DVMAR"
     gene            2056..3384
                     /gene="potE"
                     /locus_tag="ACZPB6_06790"
     CDS             2056..3384
                     /gene="potE"
                     /locus_tag="ACZPB6_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HIR1184208.1"
                     /translation="MKTIEKPKMTCLQLTMAVMLNMLGSSIILMPTKLAEVGTMSILA
                     WLVTISGATTIAYVFAKCGMFSKKSGGIGGYAAYAFGKSGSFIANFSYGVSLIIANLT
                     IAISIVGYLMVLFEWNLTPLEMGIASIIVIWLCSLPNIRGARFIGQLSHFSSYGILIP
                     ILILTVIGWNWFSVDRYIGAWNPQNLSLFDGISAGISMTLWGFLGLETASANSEAVED
                     PEKSVPRAMVLATLGAGTVYVLANNISFGIVDNELLSHSTAPFGLVFATMFSPEIGKF
                     VMALMALACAGCLTSWQFTIAEVFRVSAREEYFPKLFAKVTRLNVPVIGMVVILLLQS
                     LAALLTIDPRLEQQFYILKDLAVVTNLVPYLLAFAAANVIVHREPADPKTASRITIVA
                     TIASVYSLYALYACGESAMMWGALTTFAGIWLYGFAAKSLEIKGRLRGEE"
     gene            complement(3489..5375)
                     /locus_tag="ACZPB6_06795"
     CDS             complement(3489..5375)
                     /locus_tag="ACZPB6_06795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1184209.1"
                     /translation="MNFFPIREQTWRFLRAFFMTSTVHHITAGTLAKLILSSLKLFPV
                     ALTAQFTGMASLTAVYWQSGVSEVFLTVWFCCGLIQIWLSLRYVRLFWRDANREANIR
                     KWIRRWTALAVSAGIIWGVAGPTLMVPFQGAHQMITVATIVAVTFASWPVYSCWLPSL
                     SAFTLCSLTPVIVAGAVQFGVSQTLIALIILVTTVFILYSGRKLNEMVVNSIITAAQN
                     ERLVERLRIEIARAEKARRQTEEESERRSKFFAAANHDIRQPLQAMGIYIDILGRRAT
                     PQTVPVVKQLALSISAISTLVEQVLTVTRMEFGQMELHPEHVSVRELLEELAEECRPI
                     AEKKGLRLRVSAPPVTILTDVTMVKRALKNLVSNAVAYSDPAKARVPEIVLGARRVES
                     RLVIGVYDCGPGISAEDRSRIFGTFYRGAAGRAAPGSGYGLGLSIVRGIARQLGLELT
                     LGSKPGRGSVFRLAFSIDESEQADRFEKAVPIGGEIRALKGRILLLEDNRFVRDALKS
                     MLESWGAEVIDAGEPCEEFYRLAAETENVIALVSDYNLGAEKPTGLEAAGMIEAHMKR
                     PVPTVMLTAVAVDLIEGEYRKRRERGRVVPSSLPHILQKPADAKSLNEAIMRVRPMAE
                     NAAF"
BASE COUNT          968 a         1856 c         1628 g          998 t
ORIGIN      
        1 cctgaaattt ggagtgagcg ctactttttt ttcttggagg gagccgacca gactttttct
       61 taaaagtcaa ggccgcgttc cacggacttc cgagatgaac ctatttttcg cggtccttcg
      121 agcggaactc gaggccctcc tgaagcgaga cggcgtgctc gttgagaagc tcgctcgcgt
      181 ccttcgcgcc ctcgccggcg gcgccgagga tttcggcggc ctgacgggtg gtttcttcag
      241 gcatcttcac gatgcgctcg tggatgtgct ccttcgtgcg gtcgacgacg cccttggcct
      301 cgtcgctcaa ctcgatcgtc ttgtcggcga ccttcgagac ggcattggtg cccgcctcgc
      361 gggcggcctc gatgtcctcc ttgctgcagc ctgcgatgca ggcggcgacg gcggccgtca
      421 tgacgagtag catgctcttc ctgatcatgg atcgatccat ttcctcaagt gtcctgtgaa
      481 ttgaaggccg gcccgccgcg gcgggccggc cgtgtcacga cagtatatcg gctcgcaccg
      541 caagcgcctg cgccgcagag gctctcagct caaaaaaact tgcaagcgga tgtatgcgga
      601 gccttcatcg ggccatcacg tcgagaatgc ccgcgagctg ctcgtcgtac caaggattga
      661 aggtgagctt cgcgcagttg cgcccgtcgc ccggctgatg gcgctcgccc gaataccaga
      721 tctcggcgcc ttcctcgcag gccttggatt cggccttcga ctgaccgttc cagcagaggc
      781 gctccgcccc cgattcgccg tacatgggat tgtcgggacg atacgtcatg gacatgtgcg
      841 agaagctcgc gatgcgcttc tcgggcaccg cttcgggcag gatgcgcacg tgcgccgaaa
      901 gtccccttgc gaacctctcc tcgccgtacc agaagatgcg ggtcctcgga ttcgggaagg
      961 cgcgatcgaa cctcgcgagc aggtcctccg tctcgacgag tccgtcgtgc tccgcgagca
     1021 tcacggccgc cggaccgctc cagggcttct tctcaaggcg ctcaagcacg tcgtccgccc
     1081 gcaccatcgt gtcgtggaag gccgcaaagc ccttcatcgg caccacgaag tactggaacg
     1141 gattgcgccc gccgttggga cggctctcag gcgtcacgag ccagtccctg aagttcgaga
     1201 cgaaggacgc cgcccaagcg aacggcggcc tcacctcgac cgcgggcgag aagagcacaa
     1261 agcccttcgt cctgccgggg ttcgcgtcag cgtagtcgag cacaaggttg caccccgtcg
     1321 aatagccgcc gagccacatc tcgtcgacct ccctcgaaag gatgtcggcc tgctcctgca
     1381 cgacgcgccg ccagtcgtcc gccgtcgcgg gcatcatgtc ggcgggctgc gtgccgcagc
     1441 cgggcagaag caccgtcctc accagaatgc cgttcgccgc aagcgactgc gcaatgtcgg
     1501 agaaggacca gggcgagtcg ccgagcccgt gcacgagcag gatcccgcgc ccgtcggaat
     1561 gctcggggcg gtactccttc ggcaggttcg cctcgagctc catcttcctg ccctcgtccg
     1621 tgtcgagcac gaagctgcgg ttggcgccga tccacgcctt tgcgtcggcc gcatactgcg
     1681 cgaagctcgt ctggccgagc ggcataacgt gcctctgcgc gtcctcgagc gacggtgcgg
     1741 cacagctcgt gacggaaagc agaatcgagg cggcgaggat tccggaacgg acgaacatga
     1801 aggactcctg aaaacgggtg ggtgaggaca ctttctcacg ttcagcctga aacctcaagt
     1861 gcacgccagc cccctgcacg cgcatgtgcc gcagcgggtc tgcgacgccg cacgcccgcc
     1921 ccgtcctcga cacgctcctc aacgtgcgca gacgtcctca aaaaatcctc aacccggcgc
     1981 gccttaatcg gtacaatgcg gggtttgttt tttttcaacc aacaccaaga aagatatcca
     2041 accagagacg ccattatgaa aacgatcgaa aagccgaaga tgacctgtct gcagctcacg
     2101 atggccgtga tgctcaacat gctcggatcc tccatcatcc tgatgccgac gaagctcgct
     2161 gaagtcggca cgatgtccat cctggcctgg ctcgtcacca tcagcggtgc gaccaccatt
     2221 gcctacgtct ttgccaaatg cggcatgttc agcaagaaga gcggcggcat cggcggctac
     2281 gccgcctatg ccttcggcaa gtccggaagc ttcattgcga acttcagcta cggcgtctcg
     2341 ctcatcattg cgaacctcac aatcgccatt tcgatcgtcg gctatctgat ggtgctcttc
     2401 gagtggaacc tcaccccgct cgaaatgggt attgcaagca tcatcgtgat ctggctctgc
     2461 tcgctgccca acatccgcgg cgcgcgcttc atcggccagc tctcgcactt ctcgagctac
     2521 ggcatcctga ttccgatcct gatcctcacc gtgatcggct ggaactggtt ctcggtcgac
     2581 cgctacatcg gcgcctggaa cccgcagaac ctctcgctct tcgacggcat ctccgcaggc
     2641 atctccatga cgctctgggg cttcctcggc cttgagaccg ccagcgcgaa ctccgaggcc
     2701 gtggaggatc ccgaaaagtc ggtcccgcgc gccatggtgc tcgccacgct cggcgccggc
     2761 accgtctacg tgctcgccaa caacatcagc ttcggcatcg tcgacaacga gctcctctcg
     2821 cactcgaccg cacccttcgg cctcgtcttc gccacgatgt tcagccccga aatcggcaag
     2881 ttcgtcatgg ccctgatggc gctcgcctgc gcgggctgcc tcacgagctg gcagttcacg
     2941 atcgccgagg tcttccgcgt ctccgcccgc gaggaatact tcccgaagct cttcgccaag
     3001 gtcacccgcc tcaacgtgcc cgtgatcggc atggtcgtga tcctgctcct gcagtcccta
     3061 gccgcgctcc tcacgatcga cccgcgcctc gagcagcagt tctacatcct gaaggacctc
     3121 gccgtcgtga cgaacctcgt cccgtacctg ctcgccttcg ccgccgccaa cgtcatcgtg
     3181 caccgcgagc ccgcagaccc gaagactgcc tctcgcatca ccatcgtcgc aaccatcgcc
     3241 tcggtctact cgctctacgc gctttacgcc tgcggcgaga gcgccatgat gtggggcgcc
     3301 ctcacgacct tcgcgggcat ctggctctac ggctttgccg caaagtccct tgagatcaag
     3361 ggccgcctgc gcggcgaaga ataagcctca ccgccttcgg gtccatcccg caaccgtcaa
     3421 tgcaactcgg gcgtgtctcc cttcggagac acgcccgagt tcttttccgc ctctttggca
     3481 tcccttcatc agaacgccgc gttctcggcc atcggcctca cgcgcatgat cgcctcattg
     3541 aggctctttg cgtcagcggg cttctgaagg atgtgcggca gcgacgaggg caccacgcgg
     3601 cccctctcgc ggcgcttcct gtactcgccc tcgatcaggt cgaccgcaac ggccgtgagc
     3661 atcacggtgg gcacggggcg cttcatgtgc gcctcgatca tgcccgccgc ctcgagtccc
     3721 gtgggcttct cggccccgag gttgtagtcg ctcacgagcg cgatcacatt ttcggtctca
     3781 gcggcgagcc tgtagaactc ctcgcagggc tcgcccgcat cgatcacctc ggctccccag
     3841 ctctcgagca tgctcttgag ggcatcgcgc acgaagcgat tgtcctcgag aagcaggatc
     3901 ctgcccttga gggccctgat ctcgccgccg atcggcacgg ccttctcgaa gcggtcggcc
     3961 tgctcggact cgtcgatgct gaaggcgaga cggaagacgc tgcctcggcc cggcttcgag
     4021 ccgagcgtaa gctcaagccc gagctgtctt gcgatgccgc gcacgatcga gagccccagg
     4081 ccatagcccg agcccggcgc cgcacggccc gcagctccgc gatagaaggt gccgaagatg
     4141 cggctcctgt cctcggccga gatgccggga ccgcagtcgt agacgccgat cacgaggcgg
     4201 ctctccaccc gtcgcgcgcc gagcacgatc tcgggcacgc gggccttcgc gggatcggag
     4261 tacgcgacag cgttgctcac cagattcttg agggcgcgct tgaccatcgt gacgtccgta
     4321 aggatcgtca cgggaggcgc cgagacgcga agcctcaggc ccttcttttc cgcgatcgga
     4381 cggcactcct cggcgagctc ctcgagaagc tcacgcacgc ttacatgctc gggatggagc
     4441 tccatctgcc cgaactccat gcgcgtgacg gtgagaacct gctcgaccag ggtcgagatg
     4501 gccgaaatcg agagcgcaag ctgcttgacg acgggcacgg tctgaggcgt cgccctgcgt
     4561 ccgagaatgt cgatgtagat ccccatggcc tgaagcggct ggcggatgtc gtgattggcg
     4621 gccgcaaaga acttcgagcg gcgctcgctc tcctcctcgg tctgcctgcg cgccttctcc
     4681 gcgcgcgcga tctcgatcct gaggcgctcg acgagacgct cattctgggc ggccgtgatg
     4741 atcgagttga cgaccatttc gttgagcttc ctgcccgaat agagaatgaa gaccgtcgtg
     4801 acgagaatga tgagcgcaat gagggtctgg ctcacgccga actgcacggc gcccgccacg
     4861 atgacgggcg tgagcgagca gagcgtgaag gccgagagcg acggcagcca gcaggagtag
     4921 acgggccagc ttgcgaacgt cacggccacg atggtcgcga ccgtgatcat ctggtgcgcc
     4981 ccctggaagg ggaccatcag cgtgggcccc gctacgcccc agatgatgcc tgccgagacg
     5041 gcgagcgccg tccagcggcg gatccacttg cgaatgttgg cctcgcggtt cgcgtcacgc
     5101 cagaaaagac gcacgtagcg aagcgacagc cagatctgaa tgaggccaca gcagaaccac
     5161 acggtgagaa acacctcgct cacgccgctc tgccagtaga cggccgtgag cgacgccatg
     5221 cccgtgaact gtgccgtgag ggcaacggga aagagcttga ggctcgagag aatgagcttg
     5281 gcgagcgtgc ccgccgtgat gtggtgcacc gtgctcgtca tgaagaaggc gcgcagaaag
     5341 cgccaggtct gctcgcggat cggaaagaag ttcatggcgc ggcctccagc gacacccgtc
     5401 cctcatgcga cgaaagcctc acccgcatcc cgacgggaag cgagagcttc
//
LOCUS       DBMDTI010000128         5425 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3693_length_5425_cov_2.701916, whole genome shotgun sequence.
ACCESSION   DBMDTI010000128 DBMDTI010000000
VERSION     DBMDTI010000128.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3693_length_5425_cov_2.701916"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            41..1309
                     /locus_tag="ACZPB6_06800"
     CDS             41..1309
                     /locus_tag="ACZPB6_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR1184210.1"
                     /translation="MTQSPDEARPRVKGSQTAMAAAYLVAIVLGVCSGLWGGETIHAF
                     ADFVSSIFIRLFKFISIPIIAVSIIATLASISRSSESGRIFRHTIFYTLFTTILAATL
                     AAVLYVAMSPANVAMTAGAIAPQVAEHSYLEYIQSVIPDNFLAPFLSANVLSVLLVSA
                     AIGIAISRLPRDSRSQEVLLAFFTGAQEVLFILVKWLIRVLPIGIFGFISALVVEMNK
                     GVEIGGLGTYFGVILAANFVQMLVVLPLLMLARGVNPIRVAKGMAPALAVAFFSKSSA
                     GTLPVTMSCAENNVGVARPVSRFVLPICTTINMNGCAAFILVTVVYLMQNAGAEVTLP
                     MLAAWILISTLAAVGNAGVPMGCFFLSASLLASMNVPIMLMGVILPFYAVIDMIETTL
                     NVWSDSTVAAMVNHDLGKDEKDDEAVPAAV"
     gene            complement(1558..2889)
                     /gene="lplT"
                     /locus_tag="ACZPB6_06805"
     CDS             complement(1558..2889)
                     /gene="lplT"
                     /locus_tag="ACZPB6_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HIR1184211.1"
                     /translation="MNRGFYTIMCAQFLSSLADNALLIAAIALLTSINAPDWMTPLLK
                     LFFVVSYVCLAAWVGIFADSMPKGRVMFLTNFVKAVGCLLMLFGGHPLLAYAIVGVGA
                     AAYSPAKYGILTELLPPQKLVVANGWIEGLTVGSIILGVVLGGVLIKPEIAQSILDLF
                     HLDALGLSSFAEAAIFAIAFVYLAASAVNLAIPDTGVRYPPQKFDPVDSIKGFVKSCR
                     LLWSDRLGQISLSVTTLFWGAGAVLQFLVLKWCDHALGMDLSQGAVMQAVVSLGIAVG
                     AFLAAAKVPLKKSLNVLPMGICMGVLVVGASYFTRDIAPAGGLSLFGFELSWAVMIAG
                     LIMILVGICAGFFVVPMNALLQHRGHVLMSAGRSIAVQNFNENSSILVMLGVYSLLIK
                     ADLSVPATMMIFGVFVSISMLLVILKHRRNQAEYDSTHLIGEGTRHVTEEH"
     gene            3130..4212
                     /gene="alr"
                     /locus_tag="ACZPB6_06810"
     CDS             3130..4212
                     /gene="alr"
                     /locus_tag="ACZPB6_06810"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1184212.1"
                     /translation="MPRPIYAEIDIQALKHNLARLRESVGDRTLWAVVKANAYGHGLE
                     NAVEAFADADGFATIDLCDAERARRAGWHKRVLLLEGFFDETDIEPLQDLDVETVVHS
                     MWQIDILKRLQLREVKVHIKVNSGMNRLGFLPVEVGTIYDLLTSIPGVKVMGVVTHFA
                     NAEPSYLGDAPASVSRQLTRMGRLAHSATAACMANSAAILWHPEVGGDGVRAGVALYG
                     VSPDSHISSEELGLIPAMTLSAKIIAVQEIAPGEAVGYGSRWIAKRPTRVGIVACGYA
                     DGYPRSMPDGSPTWVEGAIAPLVGAVSMDMLEIDITDIPSAGLGSRVELWGRHLPVNR
                     VAAAAGTIGYELICALARRVPLQIKH"
     gene            4233..>5425
                     /gene="radA"
                     /locus_tag="ACZPB6_06815"
     CDS             4233..>5425
                     /gene="radA"
                     /locus_tag="ACZPB6_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR1184213.1"
                     /translation="MAPRTTKTRTEFVCNECGGTTAKWQGQCPHCKAWNTLQEFKVSQ
                     GAAARYGAGSPRSAGGFVDTTGSLQDLSDVAIEEIPRTVTGLGEFDRVMGGGLVKGCV
                     ALIGGDPGIGKSTLLLQVMARLVELGHSALYVSGEESPTQIALRAQRLQLEPRGLTLM
                     SEIELEHIESVISSRKPEYVVIDSIQTIFSSLLDSAPGSVSQVRECAARLTRMAKTVG
                     TTLIFVGHVTKDGSLAGPRILEHIVDTVLYFEGDQHSNFRLVRAFKNRFGAVNELGVF
                     AMTDHGLRGVTNPSAIFLSEYKSDIPGSSVLVTQEGTRPLLVEIQALVDATHLPAPRR
                     LALGVDSQRLAMLLAVLHRHAGVGCFDQDVFVNAVGGVKISEPAADLALLCAIYSSIR
                     NKPLRR"
BASE COUNT          981 a         1700 c         1705 g         1039 t
ORIGIN      
        1 cagcactgca gtttttcaaa aacaagaagg aaactcccag atgactcaat cccccgacga
       61 agccaggccc cgcgtcaagg gttcccagac cgccatggcg gccgcctatc tcgttgcgat
      121 cgtgctcggc gtctgcagcg gcctctgggg cggcgagact atccatgcat tcgcggactt
      181 cgtgagctcg atcttcattc gcctcttcaa gttcatcagc attccgatca tcgccgtgtc
      241 gatcatcgcg acgctcgcct cgatctcccg ttcgagcgag tctggtcgca tcttccgcca
      301 cacgatcttc tacacgctct tcacgacgat tctggcggcc acgctcgccg ccgtcctcta
      361 cgtcgccatg tctcccgcca acgtcgccat gacggcgggc gccatcgcgc cgcaggtggc
      421 ggagcactcc tatctcgagt acatccagtc cgtgattccg gacaacttcc tggcgccgtt
      481 cctctccgcg aacgtgctct ctgtcctgct cgtctccgcc gccatcggca tcgcaatctc
      541 ccgcctgccg cgcgacagcc gctcgcagga ggtgctgctt gccttcttca cgggcgctca
      601 ggaggtcctc ttcattctcg tgaagtggct catccgcgtg cttccgatcg gcatcttcgg
      661 cttcatctct gcacttgtcg tcgagatgaa caagggcgtt gagatcggcg gtctcggcac
      721 ctacttcggc gtcattctcg ccgccaactt cgtccagatg ctcgtcgtgc ttccgcttct
      781 catgcttgcc cgcggcgtca atccgatccg cgtcgccaag ggcatggcgc ctgcgcttgc
      841 cgtcgccttc ttctcgaagt cctccgccgg cacgctgccc gtcacgatgt cctgcgcgga
      901 aaacaacgtg ggcgtcgctc gccccgtctc ccgcttcgtg ctgccgatct gcacgacgat
      961 caacatgaac ggctgcgccg ccttcatcct cgttacggtc gtctacctga tgcagaatgc
     1021 gggtgccgag gtgacgctgc cgatgctcgc cgcctggatc ctgatctcga ccctcgcggc
     1081 cgtgggcaac gcaggcgttc cgatgggttg cttcttcctt tcggcgagtc ttctcgcaag
     1141 catgaacgtg cccatcatgc tcatgggcgt gatcctgccc ttctacgccg tcatcgatat
     1201 gatcgaaacg acgctcaacg tctggtccga ctccacggtc gcagccatgg tgaaccatga
     1261 tctcggcaag gatgaaaagg atgacgaggc agttcccgcc gccgtataag acagggactt
     1321 tctcctgacg tcttcaggcg gaccgttctc ccgacgtgga ggcggtccgc tttttcttgt
     1381 actcgcgttt cctaaggctt tgagggggct ttcgatgggg ggcaggtggt tgctgtgttg
     1441 tttgctggcg aggagagggc gtttggcttt ttgcgcttca tggctgggag ccatgaacgg
     1501 aaacgtgccc gtgctgtcgt gcagggcccg ttcgggaggg tgccttggtg atgctgatca
     1561 gtgctcttca gtcacgtggc gggtgccttc gccgatcagg tgggtgctgt cgtactcggc
     1621 ctgattgcgg cggtgcttga ggatgacgag cagcatggag atgctcacga agacgccgaa
     1681 gatcatcatc gtggcgggaa ccgagaggtc ggccttgatg agcagggagt agacgccgag
     1741 catcacgagg atggaggagt tctcattgaa gttctgcacg gcgatggagc gtcccgcgct
     1801 catgaggaca tggccgcggt gctgcaggag cgcgttcatc ggaacgacga agaagcccgc
     1861 gcagatgccc acgaggatca tgatgaggcc ggcgatcatg acggcccagg aaagctcgaa
     1921 gccgaagagc gagaggccgc ctgcgggcgc aatgtcgcgc gtgaagtagg aggcgcccac
     1981 gacgagcacg cccatgcaga tgcccatggg cagaacgttg agggacttct tgagaggaac
     2041 cttggcggcg gcgaggaagg cgccgacggc gatgccgagg ctcacgacgg cctgcatgac
     2101 ggcaccctga gagagatcca tgccgagcgc gtggtcgcac catttgagaa cgaggaactg
     2161 aagcactgcg cctgcgcccc agaagagcgt cgtgacggag agggagatct gaccgagacg
     2221 gtcgctccag agaagacggc agctcttgac gaagcccttg atggagtcga cggggtcgaa
     2281 cttctgcggg ggatagcgca cgcccgtgtc gggaatggcg aggttgacgg ccgaggcggc
     2341 gaggtagacg aaggcgattg cgaagatggc ggcctcggca aagctcgaca ggccgagagc
     2401 atcaagatgg aacaaatcga ggatggactg tgcgatctca ggcttgatga gaacaccgcc
     2461 gagcacaacg ccgagaatga tggaaccaac cgtgaggccc tcgatccagc cgttggcgac
     2521 aacgagcttc tgcggcggga gaagctcggt gagaatgccg tacttcgcgg gcgagtaggc
     2581 cgcagcgccc acgcccacga tcgcgtaggc gagaagtggg tggccgccga agagcatcag
     2641 caggcagccg accgccttga cgaagttcgt caggaacatg acgcggccct tgggcatcga
     2701 gtctgcgaag atgcccaccc aggcggcgag gcacacgtag ctcaccacga aaaagagctt
     2761 gaggagcggc gtcatccagt caggagcgtt gatgctcgtc agaagggcga tggcagcgat
     2821 gagcagtgcg ttgtcggcca gagacgacag gaactgcgcg cacatgatgg tatagaagcc
     2881 gcggttcata aaagcctgtg aatgagcctg aatggttgga ggtcaatccg ttaagtctac
     2941 cttgcctgag ggcgtacgta aacattcggt gcaaattctt tccgagaacg gggagaggcg
     3001 ggaggtgggc cgagtcactt tcaaattgtg aaattcgaac gaatcgcggc gcaggcgagt
     3061 aacattaacg ggtttctgca cgagacgccc cgtgcgggtt cccttgactt atgaaagaag
     3121 agtattcgag tgccccgtcc catatacgct gaaattgata ttcaggcgct gaagcacaat
     3181 cttgcccgcc ttcgtgagag tgtcggcgat cgcaccctct gggcggtcgt caaggccaat
     3241 gcctacggcc acggtcttga gaatgccgtc gaggccttcg ccgatgcgga cggctttgcg
     3301 accatcgacc tgtgcgacgc cgaacgcgcg cgccgcgcgg gctggcacaa gcgcgtgctg
     3361 cttctggagg gcttcttcga tgaaacggac atcgagccgc ttcaggacct tgacgtcgag
     3421 acggtcgtcc actccatgtg gcagatcgac attctcaagc gtctccagct gcgtgaggtg
     3481 aaggttcaca tcaaggtgaa ctcgggcatg aaccgtctgg gcttcctgcc cgtcgaggtg
     3541 ggcacgatct acgacctcct cacgtccatt ccgggcgtga aggtgatggg cgtcgtcacg
     3601 cactttgcca acgccgagcc gagctatctc ggcgatgcgc ccgcatcggt ctcccggcag
     3661 ctcacccgca tgggcaggct cgcgcattcc gccacggcgg cctgcatggc caactccgct
     3721 gccatcctct ggcatcccga agtgggaggt gacggcgtgc gtgcgggcgt tgcgctttac
     3781 ggcgtgagcc ctgactccca catttcctcc gaggagctcg gcctcattcc cgccatgacg
     3841 ctctcggcga agatcatcgc cgttcaggag attgcgccgg gcgaggccgt gggctacggc
     3901 tcgcgctgga tcgccaagcg tcccacgcgc gtgggcatcg ttgcctgcgg ctacgcggac
     3961 ggttatccac gttcgatgcc cgacggctct cccacgtggg tcgagggcgc catcgcgccg
     4021 ctcgtgggcg ccgtctcgat ggacatgctc gagatcgaca tcaccgacat cccgtccgca
     4081 ggcctcggca gccgcgtgga gctctggggc aggcacctgc ccgtcaaccg agtggcggcc
     4141 gctgcaggca cgatcggcta tgagctcatc tgcgcgcttg cacgccgcgt tccgcttcaa
     4201 atcaagcact gacaaccgac atacggcaac cgatggcacc tcgcacgacc aagaccagaa
     4261 ccgaattcgt ctgcaatgag tgcggcggca cgactgccaa gtggcaggga cagtgcccgc
     4321 actgcaaggc ctggaacacg ctccaggagt tcaaggtgtc gcagggcgcc gccgcccgct
     4381 acggtgcggg ctccccgcgc tccgcaggcg gcttcgtcga cacgacgggc tccctgcagg
     4441 acctctccga cgtggcgatc gaggagatcc cccgcaccgt gacgggactc ggcgaattcg
     4501 accgagtcat gggaggcggg cttgtgaagg gatgcgtcgc cctgatcggc ggcgaccccg
     4561 gcatcggcaa gtcgacgctt ctgctgcagg tcatggccag actcgtcgag ctcggccatt
     4621 cggcgcttta cgtttcgggc gaggaaagcc ccacgcagat cgcgctcagg gcgcagcgcc
     4681 ttcagctcga gccccgcggc ctcaccctca tgagtgaaat cgagctcgag catatcgaga
     4741 gcgtgatttc tagccgcaag cccgagtacg tcgtgatcga ctcgatccag accatcttct
     4801 caagcctgct tgacagcgcg ccgggttccg tttctcaggt gcgcgagtgc gccgcaagac
     4861 tcacgcgcat ggccaagacc gtgggtacga ccctcatctt cgtcggtcac gtcaccaagg
     4921 acggctctct tgccggtcct cgaattctcg agcatatcgt cgatacggtg ctctatttcg
     4981 agggcgacca gcactcgaac ttccgtctcg tgcgcgcctt caagaaccgc ttcggcgccg
     5041 tgaacgagct cggcgtcttc gccatgacgg atcacggtct tcgcggcgtg acgaacccgt
     5101 cggccatctt cctctccgaa tacaagtctg acattcccgg gtcgagcgtg ctggtgacgc
     5161 aggagggcac tcgcccgctt ctcgttgaga ttcaggcgct cgtcgacgcc acgcacctgc
     5221 ctgcgccgag gaggcttgcc ctcggcgtgg actcgcagcg ccttgccatg cttcttgcgg
     5281 tcctgcaccg ccacgcgggc gtgggctgct ttgatcagga cgtcttcgtc aacgccgtgg
     5341 gcggcgtgaa gatctcggag ccagcggccg acctcgcgct tttgtgcgcc atctattcga
     5401 gcattcgaaa caagcccctg cgccg
//
LOCUS       DBMDTI010000129         5366 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3734_length_5366_cov_2.627730, whole genome shotgun sequence.
ACCESSION   DBMDTI010000129 DBMDTI010000000
VERSION     DBMDTI010000129.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3734_length_5366_cov_2.627730"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="ACZPB6_06820"
     CDS             <1..881
                     /locus_tag="ACZPB6_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate-alanine antiporter-like transporter"
                     /protein_id="HIR1184214.1"
                     /translation="AGPRDKEAIVAFCGEEDTKIDLATAHSPLVSRLISVTREEVNGA
                     LIEDLHLSRMDGVNITRVNRSGVVLFPYNTLRLQIGDTLNCVGPANAVARLAAMMGNQ
                     EKRLERPNVFAIFIGIALGLVLGSVPIAFPGMPTEIKLGLAGGPLIAAILLSYYGPRF
                     HLVTYTTVSANLMLREWGLTFFLASTGLAAGDMFIEAFVSGIGWVYMALGLVISVVPM
                     AVMAVVARRFMRLNFHTIAGLIAGASTSSSALSFVNSLSERGLAVLAYSTVYPLAMFL
                     RIISGQIILMLFFSMQ"
     gene            1112..1618
                     /locus_tag="ACZPB6_06825"
     CDS             1112..1618
                     /locus_tag="ACZPB6_06825"
                     /inference="COORDINATES: protein motif:HMM:NF017394.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HIR1184215.1"
                     /translation="MNFTARLAGVAAALCLSFGAVAADNGTQPITDQDQCHSCDMWIT
                     KYPGPKAEIVTKSGHVYKFCSTKCMTCTLLRLGESDKIAGIWVNDMVGNDWEHPDATF
                     IDAKTAWYVQGSSRKATMGKSLASFSTEEAAVAFRKEFGGTLYRWNDLTKEILGCKVP
                     KKPTVPME"
     gene            1961..3229
                     /locus_tag="ACZPB6_06830"
     CDS             1961..3229
                     /locus_tag="ACZPB6_06830"
                     /EC_number="1.13.12.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HIR1184216.1"
                     /translation="MKSLEDLRLDDFRLELKGRSLVPIMQGGMGVNISTAEMAQAVAA
                     RGGIGHVSDAMVPDLADRLFGTGFTRMKALACKALERTTGEAGFHFPVEKIREAAKLY
                     LGRVMEGRTGEGRIHVNIMEKLTMNASLETLRARLLGALDAGVDGISLSAGLHAGSFA
                     LMSGHPRFRDACLGVVVSSRRALNLFMRKSAKTGRLPDYVVVEGPLAGGHLGFGADWQ
                     RFSLADIVRDVKGWLHENALRIPVIAAGGVFTGTDGVRMITEAGADGIQAATRFAVTR
                     ESGLPDAVKQRYFDARAEDIEVNGISPTGYPMRMLKSSPAINAALKPQCAAYGYMLDH
                     GGCAYLRAWREAEAAGTAQDGIREKTCLCAQMRSSRVWTLGANGARLAEAGKRDASGR
                     WVLPSTAEVFDDYRFGVADEVHARRDGCGS"
     gene            3425..4549
                     /locus_tag="ACZPB6_06835"
     CDS             3425..4549
                     /locus_tag="ACZPB6_06835"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase family protein"
                     /protein_id="HIR1184217.1"
                     /translation="MKILLIPDSFKGSLSSARLCAIMKKAALDVMPDAQVTSIPAADG
                     GEGTLDVIRNSIGGSFVVHSVTGPCGQPVSARYLSAGDTAYVELAEAAGLQHRLPGFS
                     AANTTTFGAGQLIGEALNAGHRRIVITLGGSCTTDAGCGMAAALGVRFTAPNGRNFIP
                     TGATLGAIRHIALNPFFFESGVRIEALCDVTNPLCGPNGSARVFGPQKGATPEMVETM
                     EAGMAHLATIFERNKGPKLADMPCAGAAGGCGAGVVAFLNGRLLSGSDALLDLMNFSA
                     LAADADLIVTGEGMVDDQTAGGKLVSAVAARANGTPVVVLAGGIAEGTNLEALYEKGV
                     TAVMPVTSRPMTLGKAMSGGAEGVRLTATQLFRLAGALKP"
     gene            complement(4690..5226)
                     /gene="mscL"
                     /locus_tag="ACZPB6_06840"
     CDS             complement(4690..5226)
                     /gene="mscL"
                     /locus_tag="ACZPB6_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR1184218.1"
                     /translation="MRNFLKEFQAFISKGNVMDLAVAVIIGAAFSNIVNSLVKDIVNP
                     ILGVLVGRPDFTNLFVVLKPVEGYTGPQTYEALVKAGATVFGYGAFLTAVVQFLLLAF
                     VIFWLIKVVTTIRKRLETEAAKLLKAEEEKKAAAPAPAPAPTPEDVVLLREIRDLLKS
                     GAASNAEVKAAVEKLQQQ"
BASE COUNT          926 a         1642 c         1843 g          955 t
ORIGIN      
        1 tggcgggccc gcgcgacaag gaggcgatcg tcgccttctg cggcgaggag gacacgaaga
       61 tcgaccttgc gaccgcgcac tcgccgctcg tctcccgcct catcagcgtc acgcgcgagg
      121 aggtcaacgg cgcgctcatc gaggacctgc acctctcgcg aatggacggc gtgaacatca
      181 cgcgcgtcaa ccgctcgggc gtcgtgctct ttccctacaa cacgctgcgc ctgcagatcg
      241 gcgacacgct caactgcgtg ggtccggcca acgcagtggc gcgtctggcg gccatgatgg
      301 gcaatcagga gaagcggctt gagaggccca acgtcttcgc gatcttcatc ggcatcgcgc
      361 tcggcctcgt tctgggcagc gttccgatcg cctttccggg catgcccacc gagatcaagc
      421 tcggtcttgc gggcggcccg ctcatcgcgg ccatcctgct gagctactac ggcccgcgct
      481 ttcatctcgt cacgtacacg accgtctccg ccaacctgat gctcagggag tggggcctca
      541 cgttcttcct cgcaagcacg ggcctggccg caggcgacat gttcatcgag gccttcgtga
      601 gcggcatcgg ctgggtctac atggcgctcg gcctcgtgat cagcgtcgtg cccatggccg
      661 tcatggccgt cgtcgcgcgc cgcttcatgc ggctcaactt ccatacgatc gcgggcctca
      721 tcgcaggcgc ctcgacgagc agctcggcgc tcagcttcgt caacagcctc tccgaacgcg
      781 gcctcgccgt gctcgcctac tcgaccgtct acccgctcgc gatgttcctg cgcatcatct
      841 cgggccagat catcctgatg ctcttcttct ccatgcagtg aacgaggagc taacggaggt
      901 ccgaagggct ttcccagagg tgggtgcgaa cgatgtgttc ggtaaagtcg aacgtaacga
      961 attgaagcgt tcgacagaat tgggtgaagt ataatgtctg cgacttatag acaacgagcg
     1021 tcctgcggcc ggacgccgtg acgcctccgc acgccccccg gcggtgtgcg ctgacactca
     1081 gaccagacgg gccgcacagg gagaaatcga aatgaacttc accgcaaggc ttgccggcgt
     1141 cgccgccgct ctgtgccttt ccttcggcgc cgttgccgcc gacaacggca cgcagccgat
     1201 caccgatcag gaccagtgcc acagctgcga catgtggatc acgaagtatc ccggcccgaa
     1261 ggccgagatc gtgacgaaga gcggccacgt ctacaagttc tgcagcacga agtgcatgac
     1321 ctgcacgttg cttcgcctcg gcgagtccga caagatcgcg ggcatctggg tcaacgacat
     1381 ggtcggcaac gactgggagc atcctgacgc caccttcatc gacgccaaga ccgcctggta
     1441 cgtccagggc tcgagccgca aggccacgat gggcaagtcg ctcgcctcct tcagcaccga
     1501 agaggccgcc gttgccttcc ggaaggaatt cggcggcacg ctctaccgct ggaacgacct
     1561 gacgaaggaa atcctcggtt gcaaggttcc gaagaagccg actgtgccga tggaatgatg
     1621 atcatctgaa gaggcatgcg cgctttggcg cgcatctgca tccatgcaga gcccaacggg
     1681 cccgaaatcc atgcgattcc gggcccgtcg tcattttcgg acggtatgca ggcggatgac
     1741 gcctcaaggg taggctgttc ggacaagcct tcatgtcggg cagttttgac atgattgtgc
     1801 tgcagccgtt tcgagggtga cgggataatg gggacggttg tttctagggg tttgcacgga
     1861 gaatggggcg acggggagga gagggttccg cgggcagact tttcggacgc gatgcacggg
     1921 cgccgtgcct ttgcacgcaa gcacgaaaag gacatcatag atgaaaagcc tcgaagacct
     1981 tcgacttgac gactttcgcc ttgagctcaa ggggagaagt ttggttccga tcatgcaggg
     2041 agggatgggc gtgaacattt cgaccgccga aatggcgcag gccgtggccg cgcgcggtgg
     2101 catcgggcac gtctcggacg cgatggtgcc ggaccttgcg gaccgtcttt tcgggacggg
     2161 cttcacgcgc atgaaggcgc ttgcctgcaa ggcgctcgag cgcacgacgg gcgaggccgg
     2221 gtttcacttt cccgtggaga agatccgcga ggccgcgaag ctctatctcg ggcgcgtgat
     2281 ggagggcagg acgggcgagg ggcgaattca cgtcaacatc atggagaagc tcacgatgaa
     2341 cgcctcgctc gaaaccctga gggcccgcct tctcggggcg cttgatgcgg gcgtcgacgg
     2401 gatcagcctt tcggcgggcc tgcatgcggg atcctttgcg ctcatgagcg ggcatccgag
     2461 gtttcgcgat gcgtgtctcg gggtggtggt ttcgagccgc cgggcactca atctcttcat
     2521 gaggaagtcc gcgaagacgg ggcgcctgcc tgactacgtc gtggtcgagg gcccgctcgc
     2581 gggcggacat ctgggcttcg gagcggactg gcagcgcttt tcgcttgccg acatcgtgcg
     2641 ggacgtgaag ggttggctgc atgagaatgc gcttcggatt cccgtgatcg cggcgggcgg
     2701 cgtcttcacg gggacggacg gtgtgcggat gatcacggag gcgggcgcgg acggcattca
     2761 ggccgcgacg cgctttgcgg tgacgaggga gtcggggctg cccgacgccg tcaagcagcg
     2821 ctacttcgac gcgcgggcgg aggacatcga ggtcaacggc atttcgccga cgggttatcc
     2881 gatgaggatg ctcaagtcga gtcccgcgat caacgccgcg ctcaagccgc agtgcgcggc
     2941 ctacgggtac atgcttgatc acggcggctg cgcgtatctc agggcctggc gcgaggccga
     3001 ggctgcggga acggcgcagg acggcatccg ggagaagacc tgcctttgcg cgcagatgcg
     3061 cagctcacgc gtctggacgc tcggtgcgaa cggggcaagg ctcgcggagg cggggaagcg
     3121 ggatgcctcg ggcaggtggg tgcttccgtc gacggcggag gtgttcgacg actaccgttt
     3181 cggcgtggcg gacgaggtgc atgccagacg ggatggatgc ggttcatagg ggcgcgcgga
     3241 gcgtgcggac aaaaggcgag cagatgtatc cctgcgggcg tgaatcgtac aaaacgcacg
     3301 caaaatcgat atgatgtagg gacttttttc ccgcacgccg tccgacgcgt gcggatgagg
     3361 ccggcgatgg ggctctggac gagcacgaag gccgccggcc gacttctcgc tacggaacct
     3421 gatcgtgaaa attctgctga ttcccgactc cttcaagggg agcctttcct ccgcccgtct
     3481 ctgcgccatc atgaagaagg cggcgctcga cgtcatgccg gacgcgcagg tgacgtccat
     3541 cccggccgcc gacggcggcg agggcacgct cgacgtcatc cgcaactcca tcggcggctc
     3601 cttcgtggtg cacagcgtga ccggcccctg cggccagccc gtgagcgcgc gctatctgag
     3661 cgcgggcgac acggcctatg tggagctcgc ggaggctgcg ggcctgcagc acagactgcc
     3721 cggcttctcc gccgccaaca cgacgacctt cggcgcgggc cagctcatcg gtgaggcgct
     3781 caatgcgggc caccgccgca tcgtgatcac cctcggcggc agctgcacga cggacgcggg
     3841 ctgcggcatg gccgctgcgc tcggcgtgcg cttcacggcg cccaacggcc gcaatttcat
     3901 cccaacgggc gccacgctcg gcgccatccg ccacatcgcc ctcaatccgt tcttctttga
     3961 gagcggcgtg aggatcgagg cgctgtgcga cgtgacgaat cccctgtgcg gcccgaacgg
     4021 ctccgcgagg gtcttcggcc cccagaaggg cgccacgccc gagatggtcg agacgatgga
     4081 ggccggcatg gcgcatctgg ccacgatctt cgagcgcaac aagggcccga agctcgccga
     4141 catgccctgc gcgggcgcgg ccggcggctg cggcgcgggc gttgtcgcct tcctcaacgg
     4201 caggcttctg tcgggcagcg atgcgcttct tgacctgatg aacttctccg cccttgcggc
     4261 cgatgcggac ctcatcgtga cgggcgaggg catggtcgac gaccagacgg cgggcggcaa
     4321 gctcgtcagt gcggtggcgg cccgtgcgaa cggcacgccc gtcgtcgtgc tcgcaggcgg
     4381 catcgccgaa ggcacgaatc tcgaggccct gtacgagaag ggcgtcacgg ccgtgatgcc
     4441 cgtgacgagc cgtccgatga cgctcggcaa agccatgtcg ggcggcgccg agggcgtgcg
     4501 tctcacggcc acgcagctct tcaggctcgc cggggcgctc aagccctgac ggagaagacg
     4561 tccgaaccat gattcgaaag gggggctccc gcctgaggcg ggggcccctt ttgaattttt
     4621 gcaggacgaa aaaggccgca tcgcagcgac tgcgatgcgg cctttcggtg actttcgatg
     4681 aaggaggtct tattgctgct ggagcttttc gacggcggcc ttgacctcgg cgttcgaggc
     4741 ggcgccgctc ttgaggagat cgcggatttc gcgaaggagc acgacgtcct cgggcgtggg
     4801 cgcgggggcg ggagcgggcg cggcggcctt cttctcctct tcggccttga gaagcttggc
     4861 ggcttcggtc tcgaggcgct tgcggatggt cgtgacgacc ttgatgagcc agaagatcac
     4921 gaaggcgagc agcaggaact gcacgacggc cgtgaggaag gcgccgtagc cgaataccgt
     4981 ggcgccggcc ttgacgaggg cctcgtaggt ctgggggccc gtgtagcctt caacgggctt
     5041 gaggaccacg aagaggttcg tgaagtcggg gcggccgacg agcacaccga ggatcgggtt
     5101 gacgatgtcc ttgacgagcg agttgacgat gttggagaag gcggcgccga tgatcacggc
     5161 gacggcgaga tccatgacgt tgcccttcga gatgaatgcc tggaattcct tgagaaaatt
     5221 gcgcattttg tgtcttctgg ctgtggtttg agggttgaag tcatcaccgt ggcgtcctcg
     5281 gggaacgcct gcaggaggat attttaggca tgcccgcctc tcttgtcgaa tgaggcgtgg
     5341 agcgcgtttg agccgttcgc cttcga
//
LOCUS       DBMDTI010000130         5345 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3751_length_5345_cov_1.719489, whole genome shotgun sequence.
ACCESSION   DBMDTI010000130 DBMDTI010000000
VERSION     DBMDTI010000130.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3751_length_5345_cov_1.719489"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /gene="pepT"
                     /locus_tag="ACZPB6_06845"
     CDS             <1..1138
                     /gene="pepT"
                     /locus_tag="ACZPB6_06845"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIR1184219.1"
                     /translation="EGQRELAKLLARELEALGLVEIELNEHAILTALLPANVEGAPAV
                     GWVAHLDTVPVSLSPDVKAQVIHYEGGDVLLNAQKDIWVRLEEHPELAAYAGQDIVFT
                     DGTSVLGADNKAAVANVMTMLAVVTRENRPHGDIRVAFVPDEEIGLCGSKLLDLKKFK
                     VDFAYTIDCCALGEIVWETFNACSVSIRIKGVTAHPMSAKGVLVNPLLVATDLINRFD
                     RLQTPEQTEGKEGYWWFTDCEANAAECRLTMNIRDFDRSRYDSRKAFVLEAVEAVRAQ
                     HPRAVIEAELVDVYSNIADSLGDDRSPVELLYKAADNLGITTNTIAMRGGTDGSALSA
                     KGLVTPNYFTGACNFHSYAEFLPLPSLHKSLEMTLELVRLTAGK"
     gene            1281..2291
                     /locus_tag="ACZPB6_06850"
     CDS             1281..2291
                     /locus_tag="ACZPB6_06850"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR1184220.1"
                     /translation="MPHCASKVVILATGGTIVSSGASATQMTGYSIQGLGVEDLLSAV
                     PMLADVADVEAARVSNIDSSSMTSEVWLKLANAVEEALRREDVSGVVITHGTDTMEET
                     AFFLNLVVKSEKPVVLTGAMRPATALSAEGPLNLLNAVRIAADPKAAGRGVLIALNDV
                     IVSARDATKTHPTNVATFRGPDLGMLGMIAGTHIEWIARSEKPHGAATPFTLEALEAR
                     VAADGALPRVDIVYSHVDDDGVMVRAAMAAGARAIVHAGTGNGSIHEKTEGALMEAAA
                     HGVLVVRASRTPSGMTVEGLAEWQDASFVPAGTLNPQKARVLVQLSLACGVDPALAFR
                     TF"
     gene            complement(2402..3853)
                     /locus_tag="ACZPB6_06855"
     CDS             complement(2402..3853)
                     /locus_tag="ACZPB6_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR1184221.1"
                     /translation="MTEADLTPKAPLVREDEHPQFERTMKARHLIMLSLGGVIGTGLF
                     FNTGFVIGQTGAFGTVVAYLIGALVVYLVMLSMGELCVAMPQTGSFHLYATTFIGPRT
                     GFTVAWLYWVTCTVSVGTSLTGAGLAMQYWFPNVDVWIWCLFYCLLILAMNVFTTKFF
                     AEGEFIFSLIKVVTIQIFIIAGGLAVFGVIPLSDGSPAPYFSNLTEGGLFPKGLLPLL
                     TTMIAVNFAFSGTELIGVAAGETQNPEKVIPMAVRTTVVRLVLFFIGTIVILAALLPM
                     EEASILKSPFVTVFERIGIPYAADLFNFVILTAVISAANSNLYAAGRMIWALAHDGML
                     PAWASKRTKRGLPVNAIMLSMLGGWLALFTSVFAADTVFVVLTAIVGFSVVAVWIAIS
                     VAHIGFRRQLKAEGKSASDLAWHSPLFPVVPVLAIILCSVAFIGLLFDPEQRLALYFG
                     VPFAAACMILYPVIARRRDARREAARLAEAGRH"
     gene            complement(3998..5266)
                     /gene="sstT"
                     /locus_tag="ACZPB6_06860"
     CDS             complement(3998..5266)
                     /gene="sstT"
                     /locus_tag="ACZPB6_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HIR1184222.1"
                     /translation="MTKRLSRVFRFAEPIVHGSLIRQILVALVLGAALAFVWPTAAKD
                     VGILGEIFVTALKGVAPVLVFVLVTSAIANSSMSTEMHIRPVIALYLASTFGAAMASV
                     AVSFMFPTTITLTGAAQSSAAPGEISQVLTQLLLNVVDNPVRAIANANYIGILAWAVA
                     VGMILRKSREETRIVLGDASSAVSSIVQIVIRFAPIGIFGLVSHTLATEGFQVMLEYA
                     QLLLVLLGAMAFVALVLNPFIVWVATHDNPYPLTFMVLRESGVSAFFTRSSAANIPVN
                     MDICRRLKLPESTYAISIPVGSTINMAGAAVTITVITLSAAHSLGIQVDMFSAIFLSV
                     IASVCAAGTSGVPGGSLMLIPLACGLFGIDQDTAMQVVAVGFIISVLQDSTETGLNSS
                     SDVLFTIAACYRADRIRAEQEAAKNNCGNN"
BASE COUNT         1060 a         1612 c         1824 g          849 t
ORIGIN      
        1 cgagggccag cgcgagctcg cgaagcttct cgcccgcgaa cttgaggcgc tcgggctcgt
       61 cgagatcgaa ctgaacgaac acgccatcct gacggcgctt ctgcccgcca acgtcgaggg
      121 cgctcccgcc gtgggctggg tcgcgcatct cgacacggtg cccgtgagcc tctcgcccga
      181 cgtgaaggcg caggtgattc actacgaagg cggggacgtt ctcctcaatg cccaaaagga
      241 catttgggtg cgtctcgagg agcatccgga acttgccgca tatgcgggcc aggacatcgt
      301 ctttacggac ggcacgagcg tgctcggtgc cgacaacaag gcggccgttg cgaacgtgat
      361 gacgatgctc gccgtcgtga cgagggaaaa ccgtccgcac ggcgacatcc gcgtcgcctt
      421 cgtgcccgat gaagagatcg gtctttgcgg ctccaagctc cttgacctta agaagttcaa
      481 ggtcgacttc gcctacacga tcgactgctg cgcgcttggc gaaatcgtct gggagacctt
      541 caacgcctgc tcggtctcga tccgcatcaa gggcgtgacg gcgcatccga tgagcgccaa
      601 gggcgtgctc gtcaatccgc tcctcgttgc gaccgacctc atcaacaggt tcgaccgcct
      661 gcagacgccc gagcagaccg agggcaagga aggctactgg tggttcaccg actgcgaagc
      721 caacgctgcc gagtgccgcc tcacgatgaa catccgcgac ttcgaccgca gccgctacga
      781 ttcccgcaag gccttcgtgc ttgaggcggt cgaggccgtg cgcgcacagc atccgcgcgc
      841 cgtcatcgag gccgaactcg tcgacgtcta ctccaacatc gccgacagcc tcggcgacga
      901 ccgctcgccg gtggagctcc tctacaaggc tgccgacaac ctcggcatca ccaccaacac
      961 catcgccatg cgcggcggca cggacggctc cgccctctcc gccaagggcc tcgtcacgcc
     1021 caactacttc acgggcgcgt gcaacttcca cagctatgcc gagttcctcc cgctcccgag
     1081 cctgcacaag tcgctcgaga tgacgctcga actcgttagg cttacggcag ggaaataaga
     1141 ccctgacggg aatttaacgg aggaaaaggt cctttcgccg gcggcgggag ggcctttttt
     1201 gtccggcggc gatgtatgct gttgggatca ttgaaaaaac agacaaaccc atcttcaaga
     1261 caaaagacaa aggagacacc atgccgcatt gcgcatccaa ggtcgtgatt ctcgccacgg
     1321 gcggcaccat cgtcagttcg ggcgcgtccg ccacgcagat gacgggctac agcattcagg
     1381 ggctcggcgt cgaggacctg ctctcggccg ttccgatgct ggccgacgtg gccgacgtcg
     1441 aggccgcccg ggtctcgaac atcgactcga gctcgatgac ttcggaggtc tggttgaagc
     1501 tcgcgaacgc ggtcgaggag gctcttcgcc gcgaggacgt ctcgggcgtc gtgattacgc
     1561 acggcaccga cacgatggag gagaccgcgt tcttcctcaa tctggtggtg aagagcgaaa
     1621 agcccgtcgt gctcacgggc gcgatgcgtc ccgctacggc cctttcggcc gaggggcccc
     1681 tcaaccttct caacgccgtt cgcatcgcgg ccgatccgaa ggctgcgggc cggggcgtgc
     1741 tcatcgcgct caacgacgtg atcgtctccg cgcgtgacgc cacgaagacc catcccacca
     1801 atgtcgccac cttcaggggg ccggatcttg gcatgctcgg catgatcgcg ggcacgcaca
     1861 tcgagtggat cgcgaggtcc gaaaagcccc atggggccgc cacgcccttc acgcttgagg
     1921 cgctcgaagc ccgtgttgcg gccgacggcg ccctgccccg cgtcgacatc gtgtattcgc
     1981 acgtcgacga cgacggcgtg atggttcgtg cggccatggc tgcaggcgcc cgtgcaatcg
     2041 tgcatgcggg gaccggcaac ggctcgattc acgagaagac ggaaggcgcc ctcatggagg
     2101 ctgccgctca cggcgtactc gtcgtgcgcg cttcgagaac cccgagcggc atgaccgtcg
     2161 agggactcgc cgagtggcag gatgcgagct tcgtgcctgc agggacgctc aacccgcaga
     2221 aggcccgtgt gctcgtgcag ctctcgctcg cctgcggcgt cgatcccgcc ctcgccttca
     2281 ggacgttctg aaagccttga gatgaaaaca ggaccctgac gggtcctgaa taccaaaagc
     2341 aaacggcccc gctcatcctg tcatgagcgg ggccgtttgt gttcagagag gcttcagggt
     2401 gtcagtgcct gccggcttcg gcgaggcgtg cggcctcgcg gcgggcgtcg cggcggcgtg
     2461 cgatgacggg atagaggatc atgcaggctg cggcgaaggg aacgccgaag tagagcgcga
     2521 gtcgctgctc ggggtcgaag agaagcccga tgaaggcgac cgagcagagg atgatcgcga
     2581 ggacgggcac gacgggaaag agcggcgagt gccaggcgag gtcggaggcg gacttgcctt
     2641 cggccttaag ctgcctgcga aagccgatgt gcgcgacgct gatggcaatc cagacggcga
     2701 cgacggagaa gccgacgatg gcggtgagga ccacgaagac cgtatcggcg gcgaagaccg
     2761 aggtgaagag agcgagccaa ccgccgagca tggagagcat gatggcgttg accggaaggc
     2821 cgcgcttcgt gcgcttcgag gcccaggcgg gcagcatgcc gtcgtgtgcg agggcccaga
     2881 tcatgcggcc cgcggcgtag aggttcgagt tcgcggccga gatgacggcc gtgaggatca
     2941 cgaagttgaa gaggtcggcg gcgtacggaa tgccgatgcg ctcgaagacc gtcacgaacg
     3001 ggctcttgag gatcgaggcc tcctccatcg gaagaagcgc ggcgaggatc acgatcgtgc
     3061 cgatgaagaa gagcacgagg cgcacgacgg tcgtgcgcac ggccatcgga atgaccttct
     3121 cgggattctg cgtttcacct gcagcgacac cgatgagttc ggtgcccgag aaagcgaagt
     3181 tgacggcgat catcgtcgtg agcagcggca gcaggccctt cgggaagagg ccaccttcgg
     3241 tgaggttgct gaagtaaggc gcgggagagc cgtcggagag cggaatgacg ccgaagacgg
     3301 cgaggccacc cgcgatgatg aagatctgga tcgtgaccac cttgatgagc gagaagatga
     3361 attcaccctc ggcgaagaac ttcgtcgtga agacgttcat cgccagaatc aggaggcagt
     3421 agaagagaca ccatatccag acgtcgacgt tcgggaacca gtactgcatc gcgagccccg
     3481 cgcccgtgag cgacgtgccg acgctcaccg tgcaggtcac ccagtagagc cacgcgaccg
     3541 tgaagcccgt gcgcggaccg atgaaggtcg tggcgtagag gtggaagctc cccgtctgcg
     3601 gcatcgcgac gcacagttcg cccatcgaga gcatgacgag gtagacgacg agcgcgccga
     3661 tcagataggc gacgacggtg ccgaaggcgc ccgtctggcc gatcacgaag cccgtgttga
     3721 agaaaaggcc cgtcccgatc acgccgccca gggacagcat gatcaggtga cgggccttca
     3781 tggttctttc aaattgaggg tgctcgtcct cgcgtacgag cggcgccttg ggtgtcaggt
     3841 cggcttcggt catgtgtggg aatgcctcgg ggaaatgtgg gttgcaaaaa ggcgcggacg
     3901 gcgtaaggcg accgtccgcg ccggaatgtc tggcgttgcc cctggggcgg tttcgactga
     3961 ccgcccctag gcaaaccatc gtattttaca gatattctca gttattgccg cagttgtttt
     4021 tcgcggcctc ctgctcggcc ctgattcggt cggcgcggta gcaggcggcg atcgtgaaga
     4081 ggacgtcgga ggaggagttg aggccagttt cggtcgagtc ctgcaggacg ctgatgatga
     4141 agcccacggc cacgacctgc atggcggtgt cctggtcgat gccgaagagg ccgcaggcga
     4201 gcgggatcag catgagcgag ccgccgggaa cgcccgaggt gcccgcggcg cagacgctcg
     4261 cgatgacgga gaggaagatc gccgagaaca tgtcgacctg gatgccgagc gagtgcgcgg
     4321 ccgagagcgt gatcaccgtg atcgtgacgg cggcgcccgc catgttgatc gtggagccca
     4381 cgggaatcga gatcgcatac gtggattcgg gcagcttcag gcgccggcag atgtccatgt
     4441 tgaccggaat gttcgcggcc gacgagcgcg tgaagaaggc ggagacgccg gactcgcgca
     4501 gcaccatgaa ggtgagcggg tacggattgt cgtgcgtggc gacccagacg atgaagggat
     4561 tgagcacgag cgcgacgaag gccatggcgc cgagcagcac gagaagaagc tgcgcgtact
     4621 cgagcatcac ctggaagcct tccgtggcga gcgtgtggga cacgaggccg aagatgccga
     4681 tcggcgcaaa gcggatcaca atctgcacga tcgaggagac ggccgacgag gcgtcgccca
     4741 gcacgatgcg ggtctcctcg cgggacttcc tcaggatcat gcccacggcc accgcccagg
     4801 cgaggatgcc gatgtagttc gcgttcgcaa tggcgcgcac gggattgtcg accacgttga
     4861 gaaggagctg cgtgagcact tgggagattt cgccgggcgc cgctgaggac tgcgcggccc
     4921 ccgtgagcgt gatcgtcgtc gggaacatga agctcacggc aaccgaggcc atggccgcac
     4981 cgaaggtcga cgccagatag agcgcgatca ccggacggat gtgcatctcg gtggacatgg
     5041 acgaattcgc aatcgcgctc gtcacgagca cgaagacgag gacgggggcg acgcccttga
     5101 gggccgtcac gaagatctcg ccgagaatgc cgacgtcctt cgcggccgtg ggccagacga
     5161 aggcgagcgc ggcgcccaga acgagcgcga cgagaatctg ccggatgagc gagccgtgaa
     5221 cgatcggctc ggcaaaccgg aagacgcggc taagacgctt ggtcatgatg gaaagctgct
     5281 cctgtgtgat ggtgggggtc cgagaacggg ctgaacggtt cgcggggctg gaaatcaatc
     5341 gggcc
//
LOCUS       DBMDTI010000131         5313 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3776_length_5313_cov_2.855142, whole genome shotgun sequence.
ACCESSION   DBMDTI010000131 DBMDTI010000000
VERSION     DBMDTI010000131.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3776_length_5313_cov_2.855142"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="ACZPB6_06865"
     CDS             complement(<1..502)
                     /locus_tag="ACZPB6_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014651.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorD/DmsD family molecular chaperone"
                     /protein_id="HIR1184223.1"
                     /translation="MVTQQLQDFARQSVYDALSALFLQPEAKGAYENYVEAMRAAAGL
                     AAELGTNAAPFEAVLDAPAPEPLELEREYARLFLGTCEGTVPLAESAWLHRDEIPLSQ
                     LECRKAYADAGLETAGILGVPEDHLGLQLGFLTVLILKNDPAAATAFFEAHAAKWLPA
                     FAAAVRA"
     gene            833..3205
                     /gene="ppsA"
                     /locus_tag="ACZPB6_06870"
     CDS             833..3205
                     /gene="ppsA"
                     /locus_tag="ACZPB6_06870"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HIR1184224.1"
                     /translation="MVQGRYVFPFSQLRMTDVDRVGGKNASLGELLSQLTSAGIRVPD
                     GFATTAEAFRLFLKEGGLEERIAQRLTTLDVDDVKALAAAGAEIRGWIEAAPFPAELE
                     KEIREFYEWLKDGQEEISVAVRSSATAEDLPDASFAGQQETVLNVVGIDAVFKHMKEV
                     FASLYNDRAISYRVHKGFTHAEVALSAGVQRMCRSDRGAAGVMFTLDTESGFDQVVFI
                     TASYGLGETVVQGAVNPDEFYVHKPILDAGKYPIIRKTLGSKLIKMEFESDKSVGRTV
                     RTVDVPAEDRRKFAITDEDVIELARFARIIEKHYGRPMDIEWGKDGNDGKIYILQARP
                     ETVKSQQNKADVQLRYTLKSKGRLLCQGRAIGQKIGTGPVRIVDSAAEMDRVQAGDVL
                     VTDMTDPNWEPVMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERLCENDEV
                     TVSCAEGDTGNIYEGIQEVAVEEVQRGALPEIPVKVMMNVGNPQLAFEFQAIPNKGVG
                     LARLEFIINNNIGIHPKVVLEYPNVPSELRDAAERLSAGYASPKEFFEKRIAEGVATI
                     AAAFYPKKVIVRMSDFKSNEYRKLIGGALYEPDEENPMLGFRGASRYISNQFAECFAM
                     ECRAMKFVRDEMGLTNVELMIPFVRTLNEAKRVNEIMEQYGLKRGVNGLRLNMMCEIP
                     SNAVLADQFLEYFDGFSIGSNDMTQLALGLDRDSGLVAASFDERDPAVKALLAMAIKA
                     CRERGKYVGICGQGPSDHADFAAWLMEQGIESISLNPDTVVDTWRRLAVK"
     gene            3847..4272
                     /locus_tag="ACZPB6_06875"
     CDS             3847..4272
                     /locus_tag="ACZPB6_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR1184225.1"
                     /translation="MFISGTVLWFILALIVAAVEMVTGTVYLLAVTLACAAAGAASWL
                     GLSASWQFAVCALVTVIGCALAHLLRRRKNPQADRLMNLDEGERIRVESVGTGGTAVV
                     QYRGAPWIACAEEGDLTPGTWIIARVDGTRLVLRSAALK"
     gene            4315..5259
                     /locus_tag="ACZPB6_06880"
     CDS             4315..5259
                     /locus_tag="ACZPB6_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1184226.1"
                     /translation="MNVSGFLIFTLVLVVVAVLFAFQSVKVVPQQTAWVVERLGKFHA
                     VLTPGLNFIIPFIDRVAYRHSLKEIPLDTPSQVCITRDNTQLTVDGVLFFQVTDPQRA
                     SYGTSNYVVAITQLAQTTLRSVVGRMELDKTFEERDLINKSVVSAIDEAALNWGVKVL
                     RYEIKDLTPPAVILQAMQQQITAEREKRAVVAASEGRKLEQINLATGAREAAIAQSEG
                     EKQAEINKAEGQAAATLALATATAEALRRVAAATQAEGGMNAVNLQVAEKYVEAFSQL
                     AKTNNTLIVPGNLGDISGMIASAMKIVDGAKAQLPPKA"
BASE COUNT          990 a         1782 c         1604 g          937 t
ORIGIN      
        1 gtgcgcgaac ggctgcggcg aaggcgggga gccacttggc ggcgtgcgcc tcgaagaagg
       61 cggtggcggc cgcggggtcg ttcttgagga tcaggacggt gagaaagccg agctgcaggc
      121 cgagatggtc ttcgggcacg ccgaggatgc ctgcggtctc aagaccggcg tcggcgtagg
      181 ccttgcggca ctcaagctgg gagagcggga tctcgtccct gtggagccag gcggattcgg
      241 caaggggaac cgtgccctcg cacgtgccca ggaagagtct ggcgtactcg cgttcgagct
      301 cgagcggctc gggcgcgggg gcgtcgagca ccgcctcgaa cggggcggcg ttcgtgccga
      361 gttcggccgc gagccctgcg gccgcgcgca tcgcctcgac gtaattttca taggcgccct
      421 tcgcctcggg ctggagaaag agggcggaaa gcgcgtcgta gacggactga cgcgcaaagt
      481 cctgcagttg ctgagtgacc acgggcttgc tctatatata gacggtctgg aggggggcgg
      541 tgccgcgggg gcggcgcctg ccggccggat cggatcagtt cattgtatcc gctgccgcgt
      601 gaaagtgcct cgccctactc aaaactacct agatgggaac aatgacactc tgcctgcgtc
      661 tagatttcat ctattgaggc ggaccggacc tgcggaaaat ccctgaaatt gtcctgcggg
      721 atcaaaaaat atgcagggta ggcccatttt ccggcgcata aagcgctctg aacgggtaaa
      781 atcccccaca attgaaccac cgccccggtt cttggggctt aagaggataa gcatggtaca
      841 gggtcgttac gttttcccct ttagtcagct tcgcatgacc gacgttgatc gcgtcggcgg
      901 caagaacgct tcgctcggcg aactgctcag ccagctcacg agcgccggca tccgcgtgcc
      961 ggacggtttc gccaccacgg ccgaagcctt ccgcctcttc ctgaaggaag gcggtctcga
     1021 ggagcgcatt gctcagcgtc tcaccaccct tgacgtcgac gacgtcaagg cgctcgccgc
     1081 tgccggcgcc gagatccgcg gctggattga agccgctccg ttcccggccg agctcgaaaa
     1141 ggaaatccgc gaattctacg aatggctcaa ggacggtcag gaggaaatct ccgttgccgt
     1201 tcgttcttcc gccacggctg aagaccttcc cgacgcctcc ttcgccggtc agcaggaaac
     1261 ggtgctcaac gtcgtcggca tcgacgccgt tttcaagcac atgaaggagg tcttcgcctc
     1321 cctctacaac gaccgcgcca tctcctaccg cgtccacaag ggcttcacgc acgctgaagt
     1381 cgccctgtcc gccggcgtgc agcgcatgtg ccgctccgac cgcggcgccg ccggcgtgat
     1441 gttcacgctc gacaccgaat ccggcttcga ccaggtggtc ttcatcaccg cctcctacgg
     1501 cctcggcgaa accgtggttc agggcgccgt gaatcccgac gagttctacg tccacaagcc
     1561 catccttgat gccggcaagt atccgatcat ccgcaagacg ctcggctcca agctcatcaa
     1621 gatggagttc gaatccgaca agtccgtcgg ccgcacggtc cgcacggtcg atgtcccggc
     1681 cgaagaccgc cgcaagttcg ccatcaccga cgaagatgtg atcgaactgg cccgcttcgc
     1741 ccgcatcatt gaaaagcact acggtcgtcc gatggacatc gagtggggca aggacggcaa
     1801 cgacggcaag atctacatcc ttcaggcccg tcctgaaacg gtgaagtccc agcagaacaa
     1861 ggccgacgtg cagctccgct acacgctcaa gtccaagggc cgcctgctct gccagggccg
     1921 cgccatcggc cagaagatcg gcacgggccc ggtccgcatc gtcgacagcg ccgctgaaat
     1981 ggatcgcgtt caggccggcg acgttctcgt cacggacatg accgacccca actgggaacc
     2041 ggtgatgaag aaggcctccg ccattgtgac gaaccgcggc ggtcgtacct gccacgcggc
     2101 catcatcgcc cgcgaactcg gcatcccggc cgtcgtcggc tgcggcgacg ccacggaacg
     2161 cctctgcgaa aacgacgaag tgacggtgtc ctgcgccgaa ggcgacacgg gcaacatcta
     2221 cgaaggcatc caggaagtgg ccgttgagga agtccagcgc ggcgccctcc cggaaatccc
     2281 ggtcaaggtc atgatgaacg tcggcaaccc gcagctcgcc ttcgagttcc aggccatccc
     2341 gaacaagggc gtcggccttg cccgccttga gttcatcatc aacaacaaca tcggcatcca
     2401 cccgaaggtt gttcttgagt acccgaacgt cccgagcgaa ttgcgcgacg ccgccgaacg
     2461 cctctccgcc ggctatgcca gcccgaagga attcttcgag aagcgcatcg ccgaaggcgt
     2521 ggccacgatt gccgccgcct tctacccgaa gaaggtcatc gtccgcatgt ccgacttcaa
     2581 gtctaacgag taccgcaagc tcatcggcgg cgccctctac gagccggatg aagaaaatcc
     2641 gatgctcggc ttccgcggcg cctcccgcta catctcgaac cagttcgccg aatgcttcgc
     2701 gatggaatgc cgcgccatga agttcgtgcg cgacgaaatg ggcctcacca acgtcgaact
     2761 gatgattccg ttcgtgcgta cgctcaatga agccaagcgc gtcaacgaaa tcatggaaca
     2821 gtacggcctc aagcgcggcg tcaacggcct tcgcctcaac atgatgtgcg aaatcccgtc
     2881 caacgccgtc ctcgccgacc agttcctcga gtacttcgac ggcttctcca tcggctcgaa
     2941 cgacatgacg cagctcgccc tcggtctcga ccgagactcc ggcctcgtcg ccgcgtcctt
     3001 cgacgaacgc gatccggccg tcaaggcgct cctcgccatg gccatcaagg cctgccgcga
     3061 acgcggcaag tacgtcggca tctgcggcca gggtccttcc gaccacgccg acttcgccgc
     3121 ctggctgatg gaacagggca tcgagtccat ctcgctcaac cccgacaccg tcgtcgacac
     3181 gtggcgtcgc ctcgctgtca agtaattgac gcgctgagac gctagtccga acaaggcccc
     3241 cggctccgaa aggcgccggg ggcttttgtc tggtcccttc gaatcgcatg tgcccgagat
     3301 gttttcacgt ccgtgcttga catgtgccat gagaatgatt atcattaatg atagtaagaa
     3361 ccattcgcag gtgcgaacgg gcaggaccac acgaatcaca ggcgcctttc gggcgcaggg
     3421 aagggcaaca tgacgaagca tgcagctgca aggacgcgca cggcgctcag agccgcctca
     3481 accgcaggtc tttcggtctc cgcattcttt cttgcggcgc ccgttctcgg tcttctcgcg
     3541 ccgcaggcga gctttgcggc ggcgatcgct gcctttgccg cggcgacagg actggatgcg
     3601 attcgagacc gtacgcacgg agaatagttt tcacgacttc tgcagttgag tgaggaccgg
     3661 cgcccgcagt tctcttcgga ggaatgcggg cgccggtctt tttgtcttcc cgattgtcgt
     3721 ttcgtaagtg cgtgaaaacc taggagtgcc gccgttcgcc gctgtgctag agtgttctgg
     3781 tacggcctct gtggcccgca gggcggttcc tgcggcggat caggggcgct tgcggaggca
     3841 ggaacgttgt tcatatcagg tacggttctc tggttcatac tggcgctgat cgtcgccgcc
     3901 gtcgagatgg tgacgggcac ggtctatctt ctggccgtca cgctggcgtg cgcggccgcg
     3961 ggcgccgcga gctggcttgg cctctcggcg agctggcagt tcgcggtctg cgccctcgtg
     4021 accgtcatcg gctgcgcgct tgcgcatctc ctgcgccgca ggaagaatcc gcaggccgat
     4081 cgtctcatga atcttgacga gggcgagcgc atccgagtcg aatccgtggg cacgggcggc
     4141 accgccgtcg tgcagtaccg cggcgcgccc tggatcgcct gcgccgaaga gggcgatctc
     4201 acgccgggca cctggatcat cgcccgcgtc gacggtactc gcctcgtcct tcgttccgcc
     4261 gccctcaagt agaggcgacg cctatttcac acgtcatacg gagtcattga aaaaatgaac
     4321 gtcagcggct ttctcatctt tacgctcgtg ctcgtcgtcg tggcggtgct ctttgcgttc
     4381 cagtccgtca aggtcgtgcc ccagcagacc gcgtgggtgg tcgagcgcct cggcaagttc
     4441 cacgccgtgc tcacgccggg cctcaacttc atcattccct tcatcgaccg cgtcgcctac
     4501 cgccactccc tcaaggagat cccgctcgac acgccgagcc aggtctgcat cacgcgcgac
     4561 aatacccagc tcacggtcga cggcgtcctc ttcttccaag tgaccgaccc gcagcgcgcc
     4621 tcctacggca cgtccaacta cgtcgtcgcc atcacgcagc tcgcccagac gacgcttcgc
     4681 tccgtcgtcg gcaggatgga gctcgacaag accttcgagg agcgcgatct catcaacaag
     4741 tccgtcgtct ccgcgattga cgaggcggcc ctcaactggg gcgtcaaggt gctccgctac
     4801 gagatcaagg acctgacgcc gcccgccgtc atcctgcagg ccatgcagca gcagatcacg
     4861 gccgagcgcg aaaagcgcgc cgtcgtcgcc gcatccgaag gccgcaagct cgagcagatc
     4921 aacctcgcca ccggcgcccg cgaggccgcc atcgcccagt ccgagggtga aaagcaggcc
     4981 gagatcaaca aggccgaagg ccaggcggcc gccacgctcg cccttgccac ggcaacggcc
     5041 gaggcccttc gccgcgttgc cgccgccacg caggccgaag gcggcatgaa cgccgtcaac
     5101 ctgcaggtcg ccgaaaagta cgtcgaggcc ttctcgcagc tcgcgaagac caacaacacg
     5161 ctcatcgtcc cgggcaacct cggcgacatc tccggcatga tcgcctcggc gatgaagatc
     5221 gtcgacggcg ccaaggccca gctcccgccc aaggcctgag ccgtcctttg aagcaccttc
     5281 cgaaggcgcc cgcaggattc gtccgcgggc gcc
//
LOCUS       DBMDTI010000132         5312 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3777_length_5312_cov_3.522932, whole genome shotgun sequence.
ACCESSION   DBMDTI010000132 DBMDTI010000000
VERSION     DBMDTI010000132.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3777_length_5312_cov_3.522932"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="ACZPB6_06885"
     CDS             complement(<1..364)
                     /locus_tag="ACZPB6_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1184227.1"
                     /translation="MAKLLTVEDETAIRELLAFICETDGHEVVKAASLAEAREALRRG
                     GLDLILLDVMLPDGSGFDFLREVRTDAASASVPVVMITARGDEADRVEGLDAGADDYV
                     VKPFMPRELTARIRAVLRR"
     gene            complement(396..1109)
                     /gene="phoU"
                     /locus_tag="ACZPB6_06890"
     CDS             complement(396..1109)
                     /gene="phoU"
                     /locus_tag="ACZPB6_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR1184228.1"
                     /translation="MNHIVKSYDRDLEEVLGEVTAMGRLVREQLVKAADALEKLDADE
                     ARSVMTRDAEVNGYEVSVDAKIESVFARRQPAARDLRTLIGMSRVSVDFERMGDEIRN
                     MAGSVLELKGLDDSLLENRSAIIMAVNTVAHMIDDSILALSRLDTNLAREVIARDRLV
                     DDVYRTTLRSLIVHMMQDPSSTEGAIALTWIGRSLERTGDHVKNVCEAVIYIEEGADV
                     RHEFYTGKMESCRNGSAEG"
     gene            complement(1129..1932)
                     /gene="pstB"
                     /locus_tag="ACZPB6_06895"
     CDS             complement(1129..1932)
                     /gene="pstB"
                     /locus_tag="ACZPB6_06895"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR1184229.1"
                     /translation="MTSIDLNSMTAMNLDGLPTKIAVNDLNVWYGKFHALKHITLAIP
                     EGRVTAFIGPSGCGKSTLLRTFNRMYALYPGQRAEGELMLDGVNILSGTIDDCTLRSR
                     VGMVFQRPTPFPMSIYDNIAYGIRLRENVAKSELDDRVEWALKKAALWNDVKDKLREP
                     GTSLSGGQQQRLCIARGVAVKPEVLLLDEPCSALDPISTARIEELIEELKNDYTVVIV
                     THSMQQAARVSDYTAFLYLGELIETGPTTDIFTRPQKKATEDYITGRFG"
     gene            complement(1977..2834)
                     /gene="pstA"
                     /locus_tag="ACZPB6_06900"
     CDS             complement(1977..2834)
                     /gene="pstA"
                     /locus_tag="ACZPB6_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR1184230.1"
                     /translation="MMKDLKMRNLSGLAGFTVMRGAALVCVLSLVSMLGVIFWNAMPA
                     MTWEFFTEAPRNMMSEGGIWPCIVGTFLLAAGALVIALPLGVMSAVFLNEYNLDSPVI
                     TVIRLSIANLAGVPSIIFGLFGLTFFVVFCGFGVSLLSGILTLAVLSLPIIINTTETA
                     LKQIPQDWREASLALGATRFETIMKVILPNALPGILTGAILALARAAGETAAVMYTGT
                     VFFTQKDGVSLFEPVMALPYHVYVMATSGTNIEGTRHLQYGTSLVLVALVFLMSLGAI
                     ILRERRRSR"
     gene            complement(2831..3712)
                     /gene="pstC"
                     /locus_tag="ACZPB6_06905"
     CDS             complement(2831..3712)
                     /gene="pstC"
                     /locus_tag="ACZPB6_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR1184231.1"
                     /translation="MLNYRSADTLFRRASGTVACVSVLALTAIVLFLLAQGLPIFKDV
                     PIGDFLFTTDWYPTDEPPVFGLGAMIVGSFAAAVLTAVIAVPLGVLTAAALHCAVPAW
                     AARILKPVIELMAALPSVVIGFIGMVIVAPWLQETFNLPTGLNLFNAGLMLAFMCIPT
                     IASISEDALRNVPGALREASLALGATRWETLCRVELRWAMGGIGTSVMLGLSRAIGET
                     MVVLMVAGGAGIIPESIFDPVRPMTAGIAAEMAEAAVGGEHYHALYAAGLLLFLITFA
                     FNLAAWYFTRRLSLRSA"
     gene            complement(3835..4668)
                     /locus_tag="ACZPB6_06910"
     CDS             complement(3835..4668)
                     /locus_tag="ACZPB6_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1184232.1"
                     /translation="MQNTIFSRRRVVTFVCAASVLAACGTTFAAGTVNLNGSTTVLPV
                     MQQVSEAFMQKNPGVVVTISGTGSGNGIKALRDKMTDVAMSSRDLKDKEKKDFEAHGI
                     NPVKIPVALDAIIPVVSPKNAVASLTMEQLRDLFAGKIKNWKELGGADAPVVVVGRDS
                     SSGTFESWQELVMGKTRVSQRALLQSSSGGVVQAVAGNPNAIGYIGVGYLDSQIKGLV
                     VNGVQPGAKTAQDGTWPISRELYLFTSGQPQGDAKKLVDYTLSAEGQQFVSKSGFVPL
                     K"
BASE COUNT          973 a         1508 c         1843 g          988 t
ORIGIN      
        1 cgcggcgaag gaccgcgcgg atgcgggcgg tgagctccct gggcatgaag ggcttgacga
       61 cgtagtcgtc ggcgcctgcg tccagtccct cgaccctgtc ggcctcgtcg ccgcgggcgg
      121 tgatcatcac gacgggcacc gaggcgctgg cggcgtcggt gcggacttcg cgcaggaagt
      181 cgaaccccga gccgtcaggg agcatcacgt caagcagaat gaggtcgagt ccgccccggc
      241 gcagcgcttc gcgcgcctcg gccaggctgg cggccttgac gacctcgtgg ccgtctgtct
      301 cgcagatgaa ggcgaggagc tcgcggatgg cggtttcgtc ctcgacggtg agcagttttg
      361 ccatagatgc cttgtcgcaa ttgttgatgg gcagatcagc cttcggcaga gccgtttcgg
      421 caggattcca ttttgccagt atagaactcg tgacgcacgt ccgcaccttc ctcaatgtag
      481 atgacggcct cgcagacgtt cttcacgtgg tcgcccgttc gttcgagcga gcggccgatc
      541 caagtgagcg cgatggcgcc ctcggtggag gacggatcct gcatcatgtg cacgatcagg
      601 ctcctgagcg tcgtgcggta gacgtcgtcg acgagcctgt cgcgtgcgat gacctcgcgg
      661 gcgaggttcg tgtcgagtcg cgagagcgcg aggatcgagt cgtcgatcat gtgggcgacc
      721 gtgttgacgg ccatgatgat ggcggagcgg ttctcaagga gagagtcgtc gagtcccttg
      781 agctctagga cgctgcctgc catgttgcgg atttcgtcac ccatgcgctc gaagtcgacg
      841 gagacgcggc tcatgccgat gagcgtgcgc aggtcgcggg cggcaggctg gcggcgggcg
      901 aagacggatt cgatcttcgc gtcgacggag acctcgtagc cgttgacctc ggcgtcgcgg
      961 gtcatgacgg agcgggcttc gtcggcgtcg agcttctcga gggcgtcggc cgccttgacg
     1021 agctgctcgc gcacgagcct gcccatggcg gtcacctcgc cgaggacctc ctcgaggtcg
     1081 cggtcgtagc ttttgacgat gtggttcatg atgtcgtttc ctttcgtttt agccgaagcg
     1141 gccggtgatg tagtcctcgg tcgccttctt ctgcggacgg gtgaagatgt cggtcgtggg
     1201 accggtctcg atcagttcgc cgaggtagag gaaggccgtg tagtcggaga cgcgggcggc
     1261 ctgctgcatg gagtgagtga cgatgacgac cgtgtagtcg ttcttgagct cctcaatgag
     1321 ctcctcgatg cgggcggtcg agatcgggtc aagggccgag cacggctcgt cgagcagaag
     1381 gacctcgggc ttgacggcaa cgccgcgggc gatgcaaagg cgctgctgct ggccgcccga
     1441 gaggctcgtg ccgggctcgc gcagcttgtc cttgacgtcg ttccagaggg cggccttctt
     1501 aagggcccac tcgacgcggt cgtcgagctc ggacttcgcg acgttctcgc gcaagcggat
     1561 gccgtaggcg atgttgtcgt agatgctcat cgggaacggg gtcgggcgct ggaagaccat
     1621 gccgacgcgg ctcctgagcg tgcagtcgtc gatagtgccc gagaggatgt tgacgccgtc
     1681 gagcatcagc tcgccttcgg cgcgctggcc cggatagagg gcgtacatgc ggttgaaggt
     1741 gcgcaggagc gtcgacttgc cgcagcccga cgggccgatg aaggccgtca ctcggccctc
     1801 gggtatggcg agcgtgatgt gcttgagggc atggaacttg ccgtaccaca cgttgaggtc
     1861 gttcaccgcg atcttcgtgg gaagaccgtc aaggttcatc gcggtcatgc tgttcagatc
     1921 aatgctggtc atgttcgtgt cttttcagat tcggttcaat gtcggccgca tccgtctcag
     1981 cgggagcggc ggcgttcgcg caggatgatg gcgccgaggc tcatcaggaa gacgagcgcg
     2041 acgaggacga gcgaggtgcc gtactgcagg tggcgggtgc cctcgatgtt cgtgccggaa
     2101 gtggccatca cgtagacgtg gtagggaagc gccatgacgg gctcgaagag cgagacgccg
     2161 tccttctggg tgaagaagac cgtgcccgtg tacatcacgg cggcggtttc acctgcggct
     2221 cgggcgagcg cgaggatggc acccgtgagg atgccgggga gcgcgttcgg caggatcacc
     2281 ttcatgatcg tctcgaagcg cgtggcgccc agggcgagcg aggcttcgcg ccagtcctgc
     2341 gggatctgct tgagggccgt ttccgtcgta ttgatgatga tcggcaggct caacacggca
     2401 agcgtgagga tgccggagag aaggctcacg ccgaagccgc agaagaccac gaagaacgtc
     2461 aggccgaaga ggccgaatat gatcgacgga acacccgcga ggttcgcgat cgagaggcgg
     2521 atcacggtga tgacgggcga gtcgaggttg tactcgttga ggaacacggc gctcatgacg
     2581 ccgagcggca gcgcgatcac gagggcgcct gcggcgagga ggaacgtgcc cacgatgcag
     2641 ggccagatgc cgccttcgct catcatgttg cgcggggctt cggtgaagaa ctcccaggtc
     2701 atggcgggca tggcgttcca gaagatgacg ccgagcatcg agacgagcga gagcacgcac
     2761 acgagggcgg cgccgcgcat caccgtgaag cctgcgaggc cggagagatt gcgcatcttg
     2821 agatctttca tcatgcgctc ctgagggaga gacggcgcgt gaagtaccac gcggcgaggt
     2881 tgaaggcgaa ggtgatcagg aagaggagca gacccgcggc gtagagcgcg tggtagtgct
     2941 cgccgcccac ggcggcctca gccatttcgg ccgcaatgcc cgccgtcatc ggacggaccg
     3001 gatcgaagat gctttcgggg atgatgcccg cgccgcccgc caccatcagc acgaccatcg
     3061 tttcgccgat ggcgcgcgag aggccgagca tgacggaggt gccgatgccg cccatggccc
     3121 agcgaagctc gacgcggcag agcgtctccc agcgagtggc gccaagggcg agcgaggcct
     3181 cgcgaagcgc gcccggcacg ttcctgagcg cgtcctcgga aatcgaggcg atcgtcggga
     3241 tgcacatgaa ggcgagcatc aggcccgcgt tgaagaggtt gaggccggtc gggaggttga
     3301 acgtctcctg aagccacggg gcgacgatca ccatgccgat gaagccgatg accaccgagg
     3361 gcagcgccgc catcagctcg atgacgggct tgaggattcg ggcggcccag gcggggaccg
     3421 cgcagtgaag ggccgcggcg gtgagcacgc cgagcggcac ggcgatgacg gcggtgagta
     3481 cggcggccgc aaaggagccg acgatcatgg cgccgagacc gaagaccggc ggttcgtccg
     3541 tcgggtacca gtcggtcgtg aagaggaagt ccccgatggg aacgtccttg aagatcggaa
     3601 ggccctgtgc gagcaggaag agcacgatgg ccgtgagcgc aagcacgctc acgcacgcca
     3661 cggtgcccga ggcccttctg aacagcgtgt ctgctgatct gtagttgagc atgagggatc
     3721 cttggaacag ctggatcttt tctggagcaa aagaaacgcc ggccgaatcg agggcgatcg
     3781 gccggcgtgc ttcaatcggt ttttctgacc gcttgcatct cgccggtcag gcgattactt
     3841 gagcggaacg aagccggact tggagacgaa ctgctggcct tcggcggaga gcgtgtagtc
     3901 gacgagcttc ttggcgtcgc cctgcggctg gccggaggtg aagaggtaga gttcgcggga
     3961 gatcggccac gtgccgtcct gagcggtctt ggcaccgggc tggacgccgt tcacgacgag
     4021 gcccttgatc tggctgtcga ggtagcccac accgatgtag ccaatggcgt tcggattgcc
     4081 ggcaacggcc tgaacgacgc cgccggagga gctctggagg agagcgcgct gggacacacg
     4141 ggtcttgccc atgacgagtt cctgccagga ttcgaacgtg ccggaggagg agtcgcgacc
     4201 gaccacgacc acgggagcgt cggcaccgcc gagttccttc cagttcttga tcttgccggc
     4261 gaagaggtcg cgcagctgct ccatcgtaag ggaggcgacg gcgttcttcg gggagacgac
     4321 agggatgatg gcgtcaaggg cgacagggat cttgaccgga ttgatgccgt gggcttcgaa
     4381 gtccttcttt tccttgtcct tgaggtcgcg gctcgacatg gccacatcgg tcatcttgtc
     4441 gcggagcgcc ttgatgccgt tgccggaacc cgtgccggag atcgtcacga caacgccggg
     4501 gttcttctgc atgaaggctt cggaaacctg ctgcatgacc ggaagaaccg tcgtggagcc
     4561 gttgaggttg accgtgccgg cggcaaacgt cgtaccgcag gcggcaagga cggaggcggc
     4621 gcagacgaag gtcacgacgc ggcggcggga aaagatggtg ttctgcatga tgaataagtg
     4681 ttccattcag ttttaaaagt cgtgcaggtg ctgttctcct gtacgtgaac gcagtatgaa
     4741 cacccggctt gacgcccccg tgaacatttc atgacgattt catgacaccc gaaaccccgc
     4801 cgcagggccc tttttgctta aaagcactta agaaacgttc tcatttattg agcaagatca
     4861 aatcccgagt gatttggtcg gattcttatt cggggcttaa ccacccccgg tccgagtcgg
     4921 gttagcatgt gtcacatgga aagggacatc aggggttcca atctcctccc cttcgctctg
     4981 ccagaggccg gcagcgcgat tgttcctctt ccggtccttt agcggaatct ggtagttccg
     5041 cgccccttca acgcccgacc ggcatttggt cgggcgcttt tttttgcggt ccgcgcggcg
     5101 aagacacacc ttcagaaact tcttcagaaa cttctgcccg tcaaatctcc tgtacaagaa
     5161 gccgctagtt cgttgtctga caaaattgcc tgagctaagg ccccccgaga taaaaaggtt
     5221 catctcggaa gtccgtggaa cgcggccttg acttttaaga aaaagtacag atcgtctcgg
     5281 aagccataag ctcgtcgctt aatttgcttg at
//
LOCUS       DBMDTI010000133         5252 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3843_length_5252_cov_3.083479, whole genome shotgun sequence.
ACCESSION   DBMDTI010000133 DBMDTI010000000
VERSION     DBMDTI010000133.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3843_length_5252_cov_3.083479"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2886)
                     /locus_tag="ACZPB6_06915"
     CDS             complement(<1..2886)
                     /locus_tag="ACZPB6_06915"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR1184233.1"
                     /translation="MKRMLFNATHAEETRVGIVDGQKLIDIDIETVGREARKSNIYKG
                     VVTRIEPSLEACFVNYGEERHGFLPFKEISRAYFKEGVDVRSASIREAITEGQELIVQ
                     VEKEERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELREAMEKLDLP
                     GGMSTIARTAGIGRTTEELQWDLNYLLKLWEAISEAAAPQYELVREENGRRTTQLVKT
                     SVVEGRPLRRANPPPFLIVEESNLVVRAIRDYFQPDIGEVLVDTDEIYEQARQFMSHV
                     MPDMVGRVKRYREDIPLFTRFQIEHQIETAYSRTVPLPSGGAIVIDHTEALVAVDVNS
                     ARATRGADIEETAFRTNCEAADEVARQMRLRDLGGLIVIDFIDMADTKNQRAVEQRLK
                     DALQYDRARVQMAKISRFGLMELSRQRLRPALSEGSHVTCPRCNGVGVIRDTESCALQ
                     VLRILQEEAMKEGTGAVHAQVPVDVATFLLNEKRNDITKLEARHRVPIVLIPNTKLET
                     PHYHIERLRQDDERLEDTTPSFNRAEDVAQVADDPYALKSQEDKPQRPKQVPVIKNVL
                     PRDPAPVHPQKAEEAKAEAGKSAQLQPVTQEKGLFSRIMSFLFGSKDEKKEEEKKVEE
                     NKTEGKEKSKDAGREDRRRRGGRRDSRRDASSRTRRTERTEEKADKTEKPEERIEEKK
                     RRERPARRPVAEAAPQAQDARDESVEKAEGERTQRRRSRRRRPAEAEVQAAQPEALQT
                     IVSEAQEVAPAAQAEVASEVKEEVKTEVKAAPVVEMPAEAVTKEPKADEAEAVRPEGE
                     EAVDEAQGETRRRRPRRRRRSGKAAGETAEGAAETTADAAPEAAVEAPDEVKAEEQAQ
                     AKPEAQVESRPAEDLKPAEDLFAGMDVGASELVQVETTAKAEPSDYVEAQPRGRKPVH
                     QAVEAAEEPLVQIETRSAEAEAPKTEADLLKSPEHMADVAQLITESFE"
     gene            4096..5103
                     /locus_tag="ACZPB6_06920"
     CDS             4096..5103
                     /locus_tag="ACZPB6_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1184234.1"
                     /translation="MRTDGVNWIAVGPDADGQRVDNYLARVAKGVPKSHIYRIIRAGE
                     VRVNKARVKAETKLVEGDLLRVPPIRISQKPAGRPAAVPLAEGTVPVLYEDRHLLIVN
                     KPSGIAAHGGSGISHGLIERLRASRPDEPFLELAHRLDRETSGAIIVCRSRKALVRLH
                     DMMKESGGIEKHYRLLVKGDWVNDRQHVKLPLVRYLLPSGERRVRVDQVEGVPAHTIF
                     TLLKRYGSVSYLDAELKTGRTHQIRVHALSQGFPLVGDDKYGDFDFNREVSRGLLGAP
                     LKRMFLHAARLRFTHPVTGEQLDIQAPMPEDCAALLKVLDEKGGRCHDGLCTQVRSFC
                     L"
     gene            5063..>5252
                     /locus_tag="ACZPB6_06925"
     CDS             5063..>5252
                     /locus_tag="ACZPB6_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR1184235.1"
                     /translation="MTGFAPKYDLFVFDWDGTVMDTTVLIAKGIQHAAREMGYPVPSF
                     QDACGVIGLDWRSAILKVV"
BASE COUNT          785 a         1604 c         1813 g         1050 t
ORIGIN      
        1 ctcgaagctt tccgtaatga gctgggccac gtcggccatg tgctcggggc tcttcagcag
       61 gtcggcttcc gtcttggggg cttcagcctc ggcagatcgg gtttcgatct gcacgagcgg
      121 ctcttcggcg gcttcaacgg cctgatgaac gggcttgcgg ccgcgcggct gggcctcgac
      181 gtagtcggag ggctcggcct tggccgtcgt ctcgacctga acgagttcag aggcgccgac
      241 gtccatgcct gcgaagagat cctctgcggg cttgaggtct tcggcgggcc gggactcgac
      301 ctgagcttca ggcttggcct gcgcctgttc ctcggccttc acttcgtcag gagcctcgac
      361 ggcagcttcg ggagcggcat cggcggtcgt ttcggcggcg ccttcggcag tctcgcctgc
      421 ggccttgccg ctgcgacgac ggcggcgcgg acgacggcgg cgggtttcgc cctgagcttc
      481 gtcgacggct tcctcgcctt cgggacgaac ggcttcagct tcgtcggcct tcggttcctt
      541 ggtcaccgct tcggcgggca tctcgacaac gggggcggcc ttgacttcgg tcttgacctc
      601 ttccttcacc tcggaagcga cttcagcctg agcggcgggg gcgacctcct gggcttcgga
      661 gacaatggtc tgaagggctt cgggctgtgc ggcctgaact tcggcttcgg caggacgacg
      721 gcggcgggag cggcggcgct gggtgcgttc gccctcggcc ttttcgacgg attcgtcacg
      781 ggcgtcctga gcctgcgggg cggcctcggc gaccggacgg cgggcgggac gctcgcggcg
      841 cttcttctct tcgatgcgct cttcgggctt ttctgtcttg tcggcctttt cctcggtgcg
      901 ttccgtgcgg cgggtgcggg aggaggcgtc acgacgggag tcgcggcggc caccgcggcg
      961 acggcggtct tcgcggccgg cgtccttgga cttttccttg ccttcggtct tgttttcctc
     1021 gaccttcttt tcctcttcct tcttttcatc cttgctgccg aagaggaagc tcatgatgcg
     1081 agagaagagg cccttttcct gcgtgacggg ctggagctgg gcggacttgc cggcctcggc
     1141 cttggcttcc tcggcctttt gcggatgcac gggagcggga tcgcgcggga gtacgttctt
     1201 gatgacggga acctgcttcg gacgctgggg cttgtcctcc tggctcttca gggcgtacgg
     1261 atcgtcggca acctgggcga cgtcctcggc gcggttgaaa ctcggcgtcg tgtcttcgag
     1321 gcgctcgtcg tcctgacgca ggcgctcgat gtggtagtgc ggcgtctcga gcttggtgtt
     1381 cgggatgagc acgatgggca cgcggtggcg tgcctcaagc ttcgtgatgt cgttgcgctt
     1441 ctcgttgagg aggaaggtgg cgacgtcgac gggaacctgc gcgtgcacgg cgcccgtgcc
     1501 ttccttcatg gcctcttcct gaaggatgcg caggacctgc agggcgcagc tttccgtgtc
     1561 gcggatcacg ccgacgccgt tgcagcgcgg gcaggtgacg tggctgcctt cggaaagggc
     1621 aggacgcagg cgttggcggg agagctccat caggccgaag cggctgatct tggccatttg
     1681 gacgcgggcg cggtcgtact ggagggcgtc cttgagacgc tgctcgacgg cgcgctggtt
     1741 cttcgtgtcg gccatgtcga taaagtcgat cacgatgagg ccgccaaggt cgcgcaggcg
     1801 catctggcgg gccacttcgt cggcagcctc gcagttggtg cggaaggccg tctcctcgat
     1861 gtcggcgccg cgcgtggcgc gtgcggagtt gacgtcgacg gcgacgaggg cttccgtatg
     1921 gtcgatcacg atcgcgccgc cggagggcag cgggaccgtg cgggagtagg ccgtttcgat
     1981 ctggtgttcg atctggaagc gcgtgaagag cggaatgtcc tcacggtagc gcttgacgcg
     2041 gccgaccatg tcgggcatga cgtgcgacat gaactggcgg gcctgttcat agatctcgtc
     2101 cgtgtcgacg agcacttcgc cgatgtcggg ctggaagtag tcgcggatgg cgcgcacgac
     2161 gaggttggat tcctcaacaa tcaggaaggg cggcggattg gcgcggcgaa gcgggcggcc
     2221 ttcgacgacg ctcgtcttga cgagctgcgt ggtgcggcgg ccgttttcct cgcgcacgag
     2281 ctcatactgc ggcgcagcgg cttcggagat ggcctcccag agcttgagga ggtagttgag
     2341 gtcccactgg agctcttcgg tcgtacggcc gatgccggcc gtgcgggcga tggtggacat
     2401 gccgccaggc aggtcgagct tttccatggc ctcgcgcagt tcctgacgtt cctcgccttc
     2461 aatgcggcgg gagacgccgc caccgcgcgg attgttcggc atgaggacga gatagcggcc
     2521 ggcaaggctg atgaaggtcg tgagggcggc gcccttgttg ccgcgctctt ccttttcgac
     2581 ctgaacgatc agttcctgac cttccgtgat ggcttcgcgg atggaggccg aacggacgtc
     2641 gacgccttcc ttgaagtagg cgcgggagat ttccttgaag ggaaggaagc cgtggcgctc
     2701 ttcgccgtag ttgacgaagc aggcttcgag gctgggctcg atgcgggtca cgacgccctt
     2761 gtagatgttg gacttgcggg cttcgcggcc cacggtctcg atgtcgatgt cgatgagctt
     2821 ctgtccatcg acgatgccga cgcgggtttc ttccgcgtgc gtggcattga aaagcatgcg
     2881 cttcatggca ttgcactccg gccgccactg acgcgggcgg actttggcgt gccttgaagt
     2941 gaaatggcgc ctgtccctgc attcgctgcg ggcaacggca agcgcgcctc agccgcatcg
     3001 aaggacgcag gcccgtcatg ccggacctcg ttgaggtcgg cacgggtggc gtcttccgcg
     3061 cgtttcggcg ccgacggcac gcgtgctccc atggtgcgtg tgacgcacct tttggagccc
     3121 tgcggccggc catcctggtt gctgtcgggt gcgtcaacgg cgctgtgtct gccccgatcg
     3181 gggcaactgg ggagagtctt cacgagcgga cgcgggagcg ttcgaacggg ctcaatccct
     3241 cacagcattc cgagccttct ccatcgtgcg gagcacgagg aaaagcccgc cggtgcatac
     3301 gctgataagc gcggccggag catttttcag acccgtcgga ggcttcgggc gcagatgact
     3361 ctacgcggcg cgctcaacac ccgaagggaa gacgggggag ctgcgccagt gcgtgctgcg
     3421 cggcgggaaa gagggacctc cctcgaattt cccggtccgg ggtccgctca ggccgggctg
     3481 acagcccgga gcggcagaca agtgaatttc agcttcaaag gcaacggcat cggggattgt
     3541 cgccctgatg ccgcctgcat ccctctctcg gcaggagccg tcgcaggcgc gggctgctcg
     3601 gccggcggac cggtcgcgat gcttctccgc tcgtctcgct tcggttccgg acacgaccgc
     3661 cgcaaagggg gcggacaagc gctcaagctg gcctcacggc tttgccgggg ctgcgagcct
     3721 aatgtcgggg agccgtttcg agaacggtgc ggcgcatcgc ggaggtcctg tcggacggcg
     3781 cggacgcctt ggcgagggct cggtacgccg ggtaccggga cgcctgttga acaaaatggg
     3841 ttttagcccg acgcttcgcc agtggggcgg cggacacaaa aatcgatgaa aacgtgagag
     3901 aatgcgcgtc atgacgcggc ttcgggccgc atcaatgcct gacggggcgc tttcaagcct
     3961 cgtcacgtct gaaacctgac cgcctgaagc cgtcgagcgg acggtggttc aaacgatcat
     4021 agccgacaag tataaacgat gagtggagaa aacaaagcgg aaatttccat cctgccttcg
     4081 tccgggatca cgtctttgcg tactgacggc gtcaactgga ttgcggtcgg cccggatgcg
     4141 gacggccagc gtgtcgacaa ctatcttgca cgcgttgcca agggcgtgcc caaaagccac
     4201 atctaccgca tcatcagggc tggtgaggtg cgcgtcaaca aggcgcgcgt caaggcggag
     4261 acgaagctcg tcgagggcga cctgctgcgc gtgcctccga tcaggatttc tcagaagccc
     4321 gcaggcaggc ctgcggccgt gccccttgct gaagggacgg ttcccgtcct gtacgaggac
     4381 agacatcttc tcatcgtcaa caagccttcg ggcattgccg cccacggcgg ctcgggcatt
     4441 tcgcacggtc tcatcgagcg cctgcgcgcc tcgcgtcccg acgaaccctt tcttgagctt
     4501 gcccacaggc tcgaccgcga gacctccggc gccatcatcg tctgtcgaag ccgcaaggca
     4561 ctcgtgcgcc ttcacgacat gatgaaggag agcggcggca ttgagaagca ctaccgcctg
     4621 ctcgtcaagg gcgactgggt caacgaccgc cagcacgtga agcttccgct cgtgcgctat
     4681 ctgttgccct cgggcgagcg tcgcgtgcgc gtcgatcagg tcgagggagt tcccgcccac
     4741 acgatcttca cgcttctgaa gcgctacggc tccgtcagct atcttgatgc ggagctcaag
     4801 acggggcgca cgcaccagat tcgcgtccat gcgctctccc agggcttccc gctcgtgggt
     4861 gacgacaagt acggggactt cgacttcaac agggaggtgt cgcgaggcct gctcggtgcg
     4921 cctctcaagc gcatgtttct gcacgccgcc cgcctgcgct tcacgcatcc cgtgacgggc
     4981 gagcagctcg acattcaggc gccgatgcct gaggactgtg ctgcgcttct aaaggttctt
     5041 gatgagaagg gaggccgctg ccatgacggg ctttgcaccc aagtacgatc tttttgtctt
     5101 tgactgggac ggcaccgtca tggacacgac ggttctcatc gcgaagggca tccagcatgc
     5161 cgcgcgcgaa atgggctatc ccgtgccgtc ctttcaggac gcctgtggcg tgatcggcct
     5221 cgactggcga agcgccatcc tcaaggtcgt gc
//
LOCUS       DBMDTI010000134         5216 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3875_length_5216_cov_1.665103, whole genome shotgun sequence.
ACCESSION   DBMDTI010000134 DBMDTI010000000
VERSION     DBMDTI010000134.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3875_length_5216_cov_1.665103"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="ACZPB6_06930"
     CDS             complement(<1..293)
                     /locus_tag="ACZPB6_06930"
                     /inference="COORDINATES: protein motif:HMM:NF021900.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HIR1184236.1"
                     /translation="MQLVGDELVILRRDCVEGIVACEDRCPAEAESSIALSRVLATIF
                     GYPEGVKVAHYCEEHGVTVKEVVLAMGLLNEADADRLIDPILMTDPEAMRMGS"
     gene            complement(445..680)
                     /locus_tag="ACZPB6_06935"
                     /pseudo
     CDS             complement(445..680)
                     /locus_tag="ACZPB6_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012432.6"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
     gene            complement(610..1011)
                     /locus_tag="ACZPB6_06940"
     CDS             complement(610..1011)
                     /locus_tag="ACZPB6_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012432.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR1184237.1"
                     /translation="MHPNTHVNKAQSTNDTIPSATHLAVASELDRIIEGVEVPGDVFA
                     AKAEAFRHVVKLGRTCWQDSLPHTLGEEFGGYAALMRRLAGKLRAVLPGCFEICMGRH
                     GARSRSRLHGRPLPPPFRHLWRRGPPDGVSL"
     gene            complement(990..1355)
                     /locus_tag="ACZPB6_06945"
     CDS             complement(990..1355)
                     /locus_tag="ACZPB6_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012432.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR1184238.1"
                     /translation="MEIPDDRYYGIQTSRMVKVSGCAHLPVIFYPGMHRALFRIKKAC
                     ALANAEIGALKPEISRAVVEACDRALAGEFDKEFQLDMWQAGGYTCVNMNVNEVIGNA
                     ANEILTGRKVRTPCTRTPM"
     gene            complement(1628..2269)
                     /locus_tag="ACZPB6_06950"
     CDS             complement(1628..2269)
                     /locus_tag="ACZPB6_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR1184239.1"
                     /translation="MISRRDLLCAGALAPLSTYSFAAPAYKAGKEYLTVHPMAPTPAD
                     KIEVVEFFAYTCPHCLQFHPVFHEWAQKAPEDVSIRICPVAWQPKYLPFTDTYFALEA
                     LGLLDSLSMPFFESIIYQTRSYNFDSATRDIHDFMVEAGVDGAKWDATFNSFGVKNKS
                     RNATMLWNAYQIDSTPMVGVGGKFTTGPHLAGSRQGTPAVIDYLVDQMRTLRK"
     gene            2746..3348
                     /locus_tag="ACZPB6_06955"
     CDS             2746..3348
                     /locus_tag="ACZPB6_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184240.1"
                     /translation="MTFEQKTRTLAAAAGCLAVLGSAVCEAAEEGPQQDVPVKVCPSS
                     PRQEAPAMPSLESVLAVWGFPMKMLGDGYVEYDGWVGRQEFGTVMAFWKADSRTSAWY
                     GLMNFPEDLTELSLRFKYIRTTATRFEVDCEGRRLRAVERVLTAAPFMKGELFGRRRL
                     SRNWVNIFDGRIDPKASTQIEDRDGEIFERSFAMACSRPE"
     gene            complement(3553..4056)
                     /locus_tag="ACZPB6_06960"
     CDS             complement(3553..4056)
                     /locus_tag="ACZPB6_06960"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR1184241.1"
                     /translation="MTDSTTKKPAELESLRADIDRSDEAIVGALRTRLGAVRRIAEVK
                     RLQGLPVYDAVREASLLYKLRSMAGSDVEGVALPVYRTMMAAARRFEAQSQEAGEAVS
                     GTVTLRLRSPADFTAVTEIFAVHDYVPESLSWVNPVIVLSATKPLPASMVRDLREHGI
                     RIEAQNA"
     gene            4880..>5216
                     /locus_tag="ACZPB6_06965"
     CDS             4880..>5216
                     /locus_tag="ACZPB6_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1184242.1"
                     /translation="MKLTTIAAAAALAVASMSASAATYVPGTYTYKVNAHNAAMTVAV
                     TVSKHKIEKIDWSKNLETIGVGQLALEKVGGRILEKQSLGVDGVTGATISSMALKYAV
                     GECLKQAKVS"
BASE COUNT         1009 a         1521 c         1709 g          977 t
ORIGIN      
        1 atcgatccca tcctcatggc ctcggggtcg gtcatgagga tgggatcgat aagtcggtcg
       61 gcgtcggcct cgttgagaag gcccatggcg aggacgacct ccttcacggt gacgccgtgc
      121 tcttcgcagt agtgggcgac cttcacgccc tcggggtagc cgaagatggt tgcgaggacg
      181 cgcgagagcg cgatggagct ttcggcttcg gcggggcagc gatcctcgca ggcgacgatg
      241 ccctcgacgc agtcgcgcct caggatgacg agttcgtcgc cgacgagctg catgctctcg
      301 aagagcactt gtagaaggtg gcatcccaaa cgttgaggtc aagttcgccc tcgtcgttgc
      361 catggagacg gcgacgtcgt tgccgacaac ctggtgggcg atctggatca ccatctcgga
      421 aagcgtcggg ttgatcttgc ccggcatgat ggagcttccg agactcaggg cggcagccgg
      481 acctccatga agcccgagcg tgggccgctg cgcatgaggc gcaggtccct ggtgatcttg
      541 gagagcaagg tcgcgacctc cttgacgagc cccgagaccg tcacgaagaa gtctgcgttc
      601 tgcaggccgt caaagagaga cgccgtcggg cggacctctt cgccatagat gtcggaaagg
      661 tggcggtaga gggcgtccat gtagccggga tcggctccga gccccgtgcc ggcccatgca
      721 gatctcaaag caccccggaa gcacggcacg gagcttgccg gccagacgcc gcatgagagc
      781 ggcatagccg ccgaactcct ctccgagcgt gtgcgggagc gagtcctgcc agcaggtgcg
      841 gccgagcttg acgacgtgcc tgaaggcctc agccttcgcg gcgaagacgt cgccgggcac
      901 ctcgacgcct tcgatgatgc ggtcgagctc agacgctacg gcaaggtgcg tggcggacgg
      961 aatggtgtcg ttcgtcgact gggccttgtt cacatgggtg ttcgggtgca cggcgtcctg
     1021 accttgcggc ccgtgaggat ttcgttggcg gcattgccga tcacttcgtt cacgttcatg
     1081 ttgacgcagg tgtagccgcc ggcctgccac atgtcgagct gaaattcctt gtcgaattcg
     1141 cccgcgagcg cgcggtcgca cgcctcaacg acagctcgcg aaatctcggg cttcagggcg
     1201 ccgatttcgg cgttggcgag cgcgcaggcc ttcttgatgc gaaagagcgc acggtgcatg
     1261 ccgggataaa agatgacggg aagatgggcg caccctgaga ccttcaccat ccgactggtc
     1321 tgaatgccgt agtagcggtc gtcgggaatt tccatggtgc cgaggcggtc gcgttgatgc
     1381 gggttgtggt catgacctgg tttccttcat tgaagattgg gttgaggatt cgacgggcgc
     1441 gcttcatgtc cgcccgtctg atgaagaaat cctgaatccc aaggccctca aagacgagaa
     1501 tgaacacgat ttctaccgct caaccacagt tttgctaaat ttcagcctca aacaaaacgc
     1561 ctccgcgtcg cgtgacgtcg gaggcgttcg tacaggtccg tgaggactgc gggaatgacg
     1621 ttcggaatta cttcctgagc gtgcgcatct ggtcgacgag gtagtcgatc acggcgggcg
     1681 tgccctgacg ggaaccggcg aggtgggggc cggtcgtgaa cttgccgccg acgccgacca
     1741 taggcgtgga gtcgatctgg taggcgttcc agagcatcgt ggcgttgcgg gacttgttct
     1801 tcacgccgaa gctgttgaac gtggcgtccc acttggcgcc gtcgacgccg gcttcgacca
     1861 tgaagtcatg gatgtcgcgg gtggcgctgt cgaagttgta ggagcgcgtc tggtagatga
     1921 tgctttcgaa gaacggcatg ctcaggctgt caagcagacc aagggcctcg agcgcgaagt
     1981 aggtgtccgt gaacggaagg tacttcggct gccaggcgac ggggcagatg cggatgctga
     2041 cgtcctcggg ggccttctgc gcccactcat ggaagaccgg gtggaactgc aggcagtgcg
     2101 ggcaggtgta ggcgaagaat tcaacgactt cgatcttgtc cgccggcgtg ggggccatcg
     2161 ggtgaacggt caggtactcc ttgccggcct tgtaggcggg agcggcaaag gaatacgtcg
     2221 aaagcggagc gagcgcgccg gcgcagagaa gatcacgacg ggagatcatg gcgggtagtc
     2281 cttattttta cagaaggtgc cgtatgaaaa atttgaccag acctgagttt agcgaaaatc
     2341 ccttgggcga ggcttaaaac gcttgatcag gatcaaatcc gtcaggtgcc agagacaatt
     2401 cggcacaaaa aagcgccttc gcctcacatg tgtacgacga aggcgcaggt tcggaatttc
     2461 aggctgaagc gagggcctcg tcagggcgcg ggaagcacgt gaggttgccc ttgttcgact
     2521 cgggaaggct ctcgaccatc agctcgggtc gggcgagggc acagaggtca aaaggcagcg
     2581 acttgttacg ccaaggggct cggccatcat gccgaaacgt tcccgagcca acgcaaggtc
     2641 gcgtaaagac ggcgttttca ttgcaaaaaa tcaaacagga ctaaggaaaa gtgcctaaaa
     2701 tgtcggaact cagtcaaaca gaagagcttt tttgacaacc tcaccatgac attcgaacag
     2761 aagacccgaa ccctggctgc cgccgctgga tgcctcgccg tcctcggctc cgccgtctgc
     2821 gaagccgcag aggaggggcc gcagcaggac gtgccggtga aggtttgccc gagctctccc
     2881 cgtcaggagg cgcccgcaat gccgtctctt gagagcgtgc tcgccgtctg gggctttccg
     2941 atgaagatgc tcggcgacgg ctatgtcgaa tatgacggct gggtcggccg tcaggaattc
     3001 gggaccgtca tggcattctg gaaggctgac agcaggacga gcgcctggta cggcctcatg
     3061 aactttcccg aggacctcac ggagctgagc ctcaggttca agtacatccg cacgaccgcg
     3121 acccgattcg aggtcgactg cgaaggcaga agactgcgcg ccgtcgagcg cgtgctcacc
     3181 gccgccccct tcatgaaggg cgagctgttc ggcaggcgtc gcctctctcg caattgggtc
     3241 aacatcttcg acgggcgcat cgacccgaag gcgtccaccc agatcgagga ccgcgatggc
     3301 gagatcttcg agcgaagctt cgccatggcg tgcagcagac ccgaatgatc agccgacgaa
     3361 aggccgtcca gaaggacggc ctttttcgcg cagcaacaac gggtttagcc tgttgcggcg
     3421 ggcggcagcg cgtcaaggga agcccctccg cagaataggg ttcctgtcag ggtcttttat
     3481 tttcagccat atcaaaaagg ccggcggatc gtctgctgcc ggcctcttct tatgcgttca
     3541 ggctgaacgc gctcaggcgt tctgtgcctc gatgcggatg ccgtgctcgc gcaggtcgcg
     3601 gaccatggag gcgggcaggg gcttcgtggc ggagagcacg atgacgggat tgacccagga
     3661 gaggctttca ggcacgtagt cgtgcacggc gaagatttcc gtgacggcgg tgaagtcggc
     3721 ggggctgcga agacgcaggg tgacggtgcc ggagacggct tcgcccgcct cctgactctg
     3781 ggcctcgaag cggcgggccg cggccatcat agtgcggtag acgggcaggg cgacgccctc
     3841 gacgtcggaa cccgccattg agcgcaactt gtagagaagg gacgcctcgc gcacggcgtc
     3901 gtagacggga aggccctgaa ggcgcttgac ctcggcgatg cgcctgacgg cgcccagccg
     3961 cgtgcgaagt gcgccgacga tggcttcgtc gctgcggtcg atgtcggcgc gaaggctttc
     4021 aagctccgcg ggcttcttgg tcgtgctgtc ggtcatggat gctccgtctg gtctttgttc
     4081 tgaaggcatg gagaagggga tgccggccgg tcggccctgg cgttgaggcg gtcccctgcg
     4141 gtccgcattt cacatcatgc ctgattccgc gccttgttgg tgaaatcccc tccggcctgt
     4201 tgattcgatt ccgcccttgg aaaagggccg gaaggctgca ctcaatcgaa aaggcccgcg
     4261 cggatctcaa gccgtcgcag gcctctgaag gctcgtcgaa cactgcgccg tgggccgctc
     4321 tcgacgaaga ggaatctggc agggagtttt tgtgccgccc gttctcggaa caggtcgcgc
     4381 ctcccttgag acacatagtg cctcatcgct cagcgcttcg caagggctcg ggcacagtcg
     4441 tttcaggctt tctcgctgtt cttcgacgcc catcaaggcg cctcgggtgc tctccctagg
     4501 caataccgcc caatcggcgg aaacggtcgg gaacagactg tcttttgtcg acaagatccc
     4561 ctcccaggcg ccctccatgc acgagcctga agacgccgac gcggaagatg cggatgatga
     4621 cgatgaggcc tgcagtcgga ggcgtcggta cggcgttccc ggacgcgcgg ctcaaaacaa
     4681 ccatcggact tcgtcggtca ggtcaaaaag gcgagggtta accctatgac tgcgccggag
     4741 ctttgattga tatcatcatt acgcactgcg caaggtctcc gcgttcaagc gccgccttgc
     4801 cgacctgcgc gccgactttt ttttgacgcc cattgaccac ggcttcaggc agcgcgcgca
     4861 actcaagagg agaaccacca tgaaactcac cacgatcgcc gcggccgccg cacttgccgt
     4921 cgcctcgatg tccgcttccg ccgccaccta cgtgcccggc acctacacct acaaagtgaa
     4981 cgcgcacaat gccgccatga cggtcgccgt cacggtttcg aagcacaaaa tcgagaagat
     5041 cgactggtcg aagaaccttg agacgatcgg cgtcggccag ctcgcccttg aaaaggtggg
     5101 cggcaggatt ctcgaaaagc agagtctcgg cgttgacggc gtcacgggcg ccacgatcag
     5161 ctcgatggcg ctcaagtacg ccgtgggcga atgcctcaag caggccaagg tttccg
//
LOCUS       DBMDTI010000135         5212 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3878_length_5212_cov_2.618666, whole genome shotgun sequence.
ACCESSION   DBMDTI010000135 DBMDTI010000000
VERSION     DBMDTI010000135.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3878_length_5212_cov_2.618666"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="ACZPB6_06970"
     CDS             complement(<1..328)
                     /locus_tag="ACZPB6_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184243.1"
                     /translation="MMTPIPVRAFSFAEDEFDVVVAGAGGAGLAAALSAAEQGASVLL
                     VEKMAAIGGNTLRASGLFNASDPARQEPMGITDSPSWHYEQTMASGHGRNDPAVVRRF
                     VCEALPT"
     gene            complement(364..1008)
                     /locus_tag="ACZPB6_06975"
     CDS             complement(364..1008)
                     /locus_tag="ACZPB6_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1184244.1"
                     /translation="MKQVPLIRIVDDDPSVGASFRFVLEAVGYQVRTYESAAEFLDGD
                     DPTREGCMLLDVRMPGLSGPELQTRLAEMKCDLPIIFLTAHGEVRMAVEAVQAGALDF
                     LEKPVDAEALLPLLARGCELHRRRRAVARDLKRAEVLWNELTDAERRTAELVAKGLPN
                     RQAAEILGLSEDTVRSRRASVFGKLELHNAAELSEFLHDMAELKTEAHWWRMAE"
     gene            complement(977..2833)
                     /locus_tag="ACZPB6_06980"
     CDS             complement(977..2833)
                     /locus_tag="ACZPB6_06980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1184245.1"
                     /translation="MTAFPVRLKATCLAFLFGASAAIAAETGMVAPSGDASGVVRVGL
                     VDTFTPSFYIDVYTPLIESLKARLPQYRFETKEISHADAMKPESVENIDFVIVSSGGA
                     RMLSRAGLQQIATLRKSRCADVSRSVGAVFLVSAGSPVQTIEDLRGRRVAATAPWSFE
                     GWLIPQGEIARRGFDPEDFFREVTFTEWNFPDVLTLVVTGMSDAGVLPACELEQAVRT
                     GVIQAEDVRIVGERPERAAGGCATSTELYPDLMFASSPSADPDVVKAVTVALLSMPAD
                     ANGLDWLSNTRMQGVEALLVRLKIGPYAYLRDNSITALALRWRNELAVMGLVLLLLLV
                     HHWRVNRLLKRRTAELRAEESARGAAAEALRASQENLALVERAGMASQLAAMFAHEIK
                     QPLTSIANYLTGIRLMMKMGNFDVPKWTEALSAAEHETHRAADIIERVRTVLRKETPG
                     FEPVDVRGLVGEALKHAGHLSEVVDVKTVFPDEEVLVKGDALELELVVVNFLKNAVRA
                     VADLPSRSSIVVEVLCQNEIVEVSVLDEGPAVSDEVFGALGKLTKSVSKEGLGFGLFI
                     ASSIAEMHRGHLAFARRTPQGLKASLVLPRLRELNINNQEWKHEAGAFDSHR"
     gene            complement(2922..4094)
                     /locus_tag="ACZPB6_06985"
     CDS             complement(2922..4094)
                     /locus_tag="ACZPB6_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1184246.1"
                     /translation="MHNQKQILLMIVLAALVMRGPINAVGPMAPVVRDVFDLPWEAFG
                     VLAGIPTMAFGLCSLAVLPLVAGFRSRTFAVAALTGAALGSFVRCIEWMPALFLGTLI
                     LSVSIAGLNVWAPSVIKVVFHDRQSQVMGVYTAVLSISGLLGTASVMPALEAGGVWWA
                     PFAVWGLAALAPLVWAVGLGGVPLPVPERKEKRLEKSGALVALLVWFVAIQAGLTFTV
                     ASMMPSGLEAAGFSRPDAVNAVTLYLGAMFAGSLGCGFLPVARKIYPVVGTVYLTGLW
                     IWMEGLGGFAGLMGAALVMGVLQGAFVAISLTAVTKKTPEADILRVTGLVQCGGYLLA
                     GLAPMMAGAWASTFGFGRMPVLLMILVLMWCVVAWLVAGRPDATGGAPRRKCACLP"
     gene            4321..4587
                     /locus_tag="ACZPB6_06990"
     CDS             4321..4587
                     /locus_tag="ACZPB6_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184247.1"
                     /translation="MLSNRRRFLQGAASAKVAPVTFKDRSYEPLKLPRPISLAALTIL
                     DVSPANQVLCAGNDPDINRLTDNYAELCELAHPYGLNLSIEPMP"
     gene            4744..4980
                     /locus_tag="ACZPB6_06995"
     CDS             4744..4980
                     /locus_tag="ACZPB6_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184248.1"
                     /translation="MCDITAEMPDTMDGILYQARNFRLSSGTGAADLVQLLKHLPISI
                     EVCNANLALTMSPLDRARMYLEDMVAVLNAAGEH"
BASE COUNT         1180 a         1697 c         1372 g          963 t
ORIGIN      
        1 aggttggcaa cgcttcgcac acgaagcgcc tcacaacagc cgggtcgttt cggccgtgtc
       61 ctgacgccat cgtctgttca tagtgccagg aaggggagtc tgtgataccc atcggctcct
      121 gacgggcagg atcagacgca ttgaacagac cgcttgcacg caacgtgttg ccgccgatcg
      181 ccgccatctt ctcgacaagc agcaccgacg ctccttgctc ggcggctgaa agtgcggcgg
      241 caagccccgc accgccagcc cccgccacca cgacgtcaaa ttcgtcttcc gcgaacgaaa
      301 acgctctcac agggatagga gtcatcatgg caagcgcgct cgccgcgcac aaagtccgcc
      361 tcctcattct gccatcctcc accagtgtgc ctcagttttg agctccgcca tgtcatgcag
      421 aaactcagag agttctgctg cattgtgaag ctcaagcttt ccgaacactg acgcccttcg
      481 gcttctgacg gtgtcctccg aaaggcccag aatttcagcc gcctgccggt tggggagccc
      541 cttggcgaca agctctgccg tccgccgttc cgcatcagtg agttcgttcc agagaacctc
      601 agcccgcttc aaatcccttg cgacagccct tctccgccta tgaagttcac accctcgtgc
      661 gagaagcggc agaagcgctt cggcatcgac aggcttttcg agaaagtcga gggcccctgc
      721 ctgcacggcc tccaccgcca tgcggacttc gccgtgagcc gtcagaaaga tgatcggcag
      781 atcacacttc atctccgcga gtcttgtctg aagttcggga ccggacagcc caggcatgcg
      841 cacgtcgaga agcatgcagc cttcgcgggt aggatcatca ccgtcaagaa actcagctgc
      901 ggattcatag gtcctcacct gatagcccac ggcctcaagc acaaatctga aggatgctcc
      961 cacgctagga tcgtcgtcaa cgatgcgaat caaaggcacc tgcttcatgt ttccattcct
     1021 gattgttgat gttgagctcc cgcagtcgcg ggagcacgag cgaggccttc agcccctggg
     1081 gagttctgcg cgcaaaggcg agatggcccc gatgcatctc ggcaatagag gatgcaatga
     1141 aaagtccgaa cccaagtcct tccttgctaa cgcttttcgt aagtttgccc agcgctccga
     1201 acacctcatc ggagacggca ggtccttcgt cgagaacgct cacctcaacg atctcgttct
     1261 gacagaggac ctcaacgaca atcgagctcc gagagggaag atcagcaacc gccctgaccg
     1321 cattcttgag gaagttcaca accacgagct ccagttcaag cgcatccccc ttcaccagta
     1381 cctcttcgtc aggaaacacg gtctttacgt cgaccacctc ggacaagtgc cccgcatgct
     1441 tcagcgcctc accgacaaga ccccgtacgt cgacgggctc gaatccaggc gtttcctttc
     1501 gaagaaccgt gcgaacgcgc tcgatgatgt ccgccgccct gtgcgtctcg tgctcggctg
     1561 ccgacagagc ctcggtccac ttgggaacat caaagtttcc cattttcatc atcagcctga
     1621 tacccgtcag ataattggcg atgctcgtca gaggctgctt gatctcgtgt gcaaacatgg
     1681 cggcgagttg agaggccatg cctgcacgct caacaagcgc gaggttctcc tgactggccc
     1741 tgagggcttc ggcggccgct cctctcgcac tctcctcagc cctgagttct gccgtcctgc
     1801 gcttgagaag acggttgacg cgccagtgat gcacaagcag aagaagcaga accagcccca
     1861 tgaccgcaag ctcattgcgc caacggagcg caagtgccgt gatcgaattg tccctcagat
     1921 aggcgtatgg accgattttc agccgaacaa gaagcgcctc caccccctgc atgcgagtgt
     1981 ttgacagcca gtcaagaccg ttggcatcgg caggcatcga aagaagggcc acagttactg
     2041 ccttcaccac atcgggatcc gccgatgggg aagaggcgaa cattaggtcg ggatagagtt
     2101 cggtcgaagt tgcacagccg cctgccgccc tttcgggacg ttcgccgacg atcctcacat
     2161 cctcggcctg tataacgcct gttctgaccg cctgctcaag ctcacacgca ggaagcacgc
     2221 ccgcatcgct catgcccgtc accacaagcg tgagcacatc gggaaagttc cattctgtga
     2281 aagtcacttc acgaaagaag tcctcgggat cgaacccacg tctggcgatc tctccttgag
     2341 gaatgagcca cccctcaaaa gaccaaggtg ccgtcgcggc caccctgcgg ccccgcaggt
     2401 cttcgatcgt ctgcacggga cttcccgccg atacgagaaa gactgcgcct acactgcggc
     2461 taacatctgc acatcgactc ttgcgaagcg ttgcaatctg ctggagcccc gcacgcgaaa
     2521 gcatgcgcgc tcctccacta gacacgatga cgaagtcgat gttctccaca gactcgggct
     2581 tcattgcatc ggcatgagaa atctccttcg tctcgaacct gtactgcggc agtctggcct
     2641 tgagcgactc gataagcggc gtatagacgt caatataaaa gctcggtgta aaggtgtcga
     2701 caagtccaac acgcacaaca cctgaagcgt ccccggaggg tgcgaccatc cccgtctcag
     2761 cggcaattgc ggcggaggcg ccaaaaagaa atgccagaca tgtcgcttta aggcggacgg
     2821 gaaatgcagt cacgacgtac agtgtcctca agttcgcttc ggataattct aaaagcttca
     2881 gctcttactg aggctcctca tcgttcacgt ctgatcttca gtcagggtag acacgcacat
     2941 ttacgccgag gcgcgccacc cgttgcatca ggtcttcccg caacaagcca cgccaccacg
     3001 caccacatga gcacgaggat catgagaagc acgggcatgc ggccgaatcc gaaggtcgaa
     3061 gcccaggcgc ccgccatcat gggtgcaagg ccggccagca ggtaaccgcc gcactgaacg
     3121 agccccgtca cgcgaagaat gtcagcttcc ggagtcttct tcgtcacggc ggtaagcgaa
     3181 atcgccacga atgctccctg cagcacgccc attacaagcg ccgctcccat gaggcctgcg
     3241 aatcctccca agccctccat ccagatccag agccccgtca gataaacggt gccgaccaca
     3301 ggatagattt ttctagccac aggcagaaag ccgcatccga gagatccggc aaacatggcg
     3361 ccaaggtaga gcgtcacggc attgaccgcg tcaggtctgg aaaagcccgc agcctcaagc
     3421 cctgagggca tcatcgacgc aacggtgaag gtgagccccg cctgaatagc aacgaaccac
     3481 acgagaagtg caacgagagc tccgctcttc tcgagacgct tttccttccg ttctggaacg
     3541 ggcagaggaa cgcctcccaa acccactgcc cagacaagcg gcgcaagagc cgccaatccc
     3601 caaactgcaa aaggagccca ccagacgccg cccgcctcca gagccggcat gaccgaggcc
     3661 gtgcccagca ggccgctgat gctgagcact gccgtataga cgcccatcac ctgagactgg
     3721 cggtcatgaa agaccacctt gatgacagag ggagcccaga cgttcaggcc cgcgattgaa
     3781 acgctcagaa tcagcgttcc gagaaagaga gcgggcatcc actcgatgca tcggacgaag
     3841 gatccgagtg cggctccggt aagtgcggcg acggcgaagg ttctacttct gaatccggcc
     3901 acaagcggca ggacagcgag gctacagagt ccaaaggcca tggtcggaat gcctgcgagt
     3961 acgccgaaag cctcccacgg cagatcgaag acatcgcgaa cgacgggcgc catcggaccc
     4021 actgcattga tgggcccgcg catgacaagt gcggcaagga cgatcatgag gagaatttgc
     4081 ttttggttgt gcatcagaag ccttcggaaa agtgattact gaaaaggcta tcccttcgtg
     4141 tgtgccttga ccagattgtt tttcccgaac agagggtttt cgccaccgct cgcggccacc
     4201 tcccacgagc cgtatcggcg ttcggattag ttctttagga tgaaggacat gcttagggcg
     4261 cggggccgga cacggtccct ggagggcgcc tcaaccatat caaccaagga gaaaaacatc
     4321 atgctttcga atcgccgccg cttccttcag ggtgccgcct ccgcgaaggt cgcccccgtc
     4381 acgttcaagg accgcagcta cgagccactg aagcttccgc gtccgatcag tctggccgcc
     4441 ctcaccattc tcgacgtctc tcccgccaat caggtgctct gcgcaggcaa cgaccccgac
     4501 atcaaccgcc tgacggacaa ctacgccgaa ctctgcgagc tcgcccatcc gtacggcctc
     4561 aacctcagca tcgagccgat gccctgatgc aacgtctcga ccgtcaagca gcagggtgag
     4621 atcctccgca agatcaaccg tccgaacgca ggctgcctcg tcgacccgat ccatttctac
     4681 cgcgccaaga acgactacaa ggacatcgac aacctgcccg ccggctcgct caagtactgc
     4741 cagatgtgcg acatcacggc cgaaatgccc gacacgatgg acggcatcct ctatcaggcc
     4801 cgcaacttcc gcctgtcttc gggcaccggc gctgccgacc tcgtgcagct tctcaagcac
     4861 ctgccgatct ccatcgaggt ctgcaacgcg aaccttgccc tgacgatgtc tccgcttgac
     4921 cgcgcccgca tgtacctcga ggacatggtc gccgtgctca acgccgcagg cgaacactga
     4981 tcctcacaag gtctctcagc aagttctccc atttcgtgca ttccgcacag gcggattcct
     5041 tccggaatcc gtcgcttttt tgagggactg gtccctgaaa aaaaggttca tctcggaagt
     5101 ccgtggaacg cggccttgac ttttaagaaa aagtctggtc ggctccctcc aagaaaaaaa
     5161 gtagcgctca ctccaaattt caggcagatt caagttacta gagaagaacc gc
//
LOCUS       DBMDTI010000136         5103 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3961_length_5103_cov_2.370652, whole genome shotgun sequence.
ACCESSION   DBMDTI010000136 DBMDTI010000000
VERSION     DBMDTI010000136.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3961_length_5103_cov_2.370652"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(62..451)
                     /locus_tag="ACZPB6_07000"
     CDS             complement(62..451)
                     /locus_tag="ACZPB6_07000"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR1184249.1"
                     /translation="MIKITKFGGSSVADAEQFKKVKAIIEADPSRRFVVSSASGKRFK
                     GDNKITDLLYLCQAHVTYHVDYLPLFNMIADRFRSIRDELGLKVDIETDLAKMRDSLP
                     EMSVDEIVSRGEYFTSRLMADYLGFPI"
     gene            complement(458..1534)
                     /locus_tag="ACZPB6_07005"
     CDS             complement(458..1534)
                     /locus_tag="ACZPB6_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR1184250.1"
                     /translation="MNIGLLGYGTVGRTVAELLDERDCGIRIRHILRRPGHADGPLMT
                     DSFEAVLNDPEVTCIVEALPGRDPALSYIRAALAAGKHVVTSNKAAVAADLKGLAELA
                     RANNVSLLFEASCGGGMHWIEMIKKTARFNEVSDVSGILNGTCNYMLDHMERSGLSFE
                     EALARAQALGYAEADPTADITGADVRNKAIISASLAYRTPVVSDIPTAGIVGVTSGII
                     AAARERGRSLRLMMLSERRGDRYAVGIVPVLLSQDEIAAHVHDNLNYGRITGDVVGAL
                     SIVGQGAGGRPTADAMIQDLISLGRGETGRPVLDRPLTYDPALLCGTGHFPDEVLPGR
                     PLAELIEIARQKQSFLAFEPQATE"
     gene            1760..3238
                     /gene="thrC"
                     /locus_tag="ACZPB6_07010"
     CDS             1760..3238
                     /gene="thrC"
                     /locus_tag="ACZPB6_07010"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR1184251.1"
                     /translation="MIYHSTRSLENGVNAREALLKGLCDDGGLYVSDELLTCVLTPDM
                     LKGLTYRETALRVFSVLLSDFTKTELAEGIERAYADNFASVAVTPVTRVGDEFLLELH
                     HGPTSAFKDVALCMLPQLMSAALKDTGRRVMIATATSGDTGKAALSGFMNVPGIGITV
                     FYPHGKVSDIQYLQMATQEGRNVAVAAVEGNFDDVQSTVKKIFASDLRQELADEGVEL
                     SSANSINIGRLVPQVVYYFDAYRQLVEANEVEQGAKVDFCVPTGNFGDVLAGYYAYRM
                     GLPVRHFIVASNANNVLTDFLTTGVYDRNRPFVKTITPSMDILISSNLERMLYYASEG
                     DTALIARLMENLRNEGVFRVEGEMLERIRSLFKCGWADDAETSAMIREAWRKDGRLVD
                     PHTAVALKVARERADRSVPCVVLSTASPFKFCRDVYIALTGRPLEGDPDGFAYMDALS
                     GLTSENAPAPLAGLRSMPVLHKAVVRPEGMADFIRAAVARAF"
     gene            3254..4138
                     /gene="thrB"
                     /locus_tag="ACZPB6_07015"
     CDS             3254..4138
                     /gene="thrB"
                     /locus_tag="ACZPB6_07015"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR1184252.1"
                     /translation="MKVTVRVPASTSNLGPGYDSLGMALSLQAKFTFEPSDELVIEGC
                     PEAYRNADNLVMQGFRMVCEKAGRPVPAVHLVIDSEVPVARGLGSSSTCIAGGMAAAN
                     AMLGNPFTTDDLFQLCTAFEGHPDNAAPAFLGGLTASFMHEGRAVAVPFSPDPAWRFV
                     AVIPNYEVRTEEARRIVRRDIDLSAAVHTMSHAIAMVRGLETGDEALVGAACADVLHE
                     PYRRTLIAEYDAMRAMALEAGAATFFISGSGSTMIAATKSEAVAEAFVAKVRETWPDF
                     AARVLTVCHEGTTVTVED"
     gene            complement(4237..4932)
                     /locus_tag="ACZPB6_07020"
     CDS             complement(4237..4932)
                     /locus_tag="ACZPB6_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184253.1"
                     /translation="MQKTLTFTITLDEIEAGKAAEVLAGAGLTPEGVIRQLLERTARE
                     GVVPLRKGRARPKRTDGGDAGAHFVQGDLFARPDPAEPFYSFNPPAAKPETLLPSLVT
                     LRALQDAQAKLRWRERERESADGSMLQSGPDAPLELLETKQFRFDKSLIVGDPRRSAI
                     IAALEALFTELTNRRVPEGLRPVGNGDSLYAVLDVESAPLGLILQIGGAEICLVRYGT
                     PESLLENPHVLEH"
BASE COUNT          866 a         1630 c         1691 g          916 t
ORIGIN      
        1 agcgaccgtg aagccgtaga gaaggtggtg cgcaatgccc ccgaacaaat caaggagctt
       61 atcaaatggg gaacccgagg tagtctgcca tcaggcggga ggtgaagtac tcgccgcggc
      121 tgacgatttc gtcgacggac atttcgggaa ggctgtcgcg catcttcgcg agatcggtct
      181 cgatgtcgac cttcaggccg agctcgtcgc gaatgctgcg gaagcggtcg gcgatcatgt
      241 tgaagagcgg cagatagtcg acgtgatagg tcacgtgggc ctggcagaga tagagcaggt
      301 cggtgatctt gttgtcaccc ttgaagcgct tgccggaggc cgaggagacg acgaatcgtc
      361 gggacggatc ggcctcgatg atggccttga ctttcttgaa ttgttcggcg tcggcgacgc
      421 tcgagccgcc gaatttggtg attttgatca tgttggatta ctccgtggcc tgaggttcaa
      481 aggcaaggaa ggactgcttt tgacgcgcga tctcgatgag ctcggcgagg gggcgtcccg
      541 gaaggacttc gtccgggaag tggcctgtgc cgcagagcag ggcggggtcg tacgtcaggg
      601 ggcggtcgag aaccggcctg ccggtctctc cgcgtccgag ggaaatcagg tcctgaatca
      661 tggcgtcggc cgtgggcctg ccgcccgcgc cctgcccgac gatcgagagg gcgcccacca
      721 cgtcgccggt gatgcggccg taattcaggt tgtcgtgaac gtgtgcggcg atttcgtcct
      781 gggaaagaag caccggcacg atgccgacgg cgtagcgatc gccgcgcctt tcgctgagca
      841 tcatgaggcg gaggctgcgg ccacgctcgc gggccgcggc gatgatgccg ctcgtcacgc
      901 ccacgatgcc cgccgtcgga atatcgctca cgacgggggt gcggtaggca agcgaggcgg
      961 agatgatcgc cttgttgcgg acgtcggcgc ccgtgatgtc ggccgtcggg tcggcttcgg
     1021 cgtagccgag ggcctgggcg cgtgcgagcg cctcctcgaa ggagaggccg ctcctctcca
     1081 tgtggtcgag catgtagttg caggtgccgt tgaggatgcc cgagacgtcg gagacctcgt
     1141 tgaagcgggc ggtcttcttg atcatctcga tccagtgcat gccgcctcca cagctcgctt
     1201 cgaagagaag cgagacgttg ttggcgcggg caagctcggc gagtcccttg agatcggcgg
     1261 ccacggcggc cttgttggat gtgacgacgt gcttgccggc cgcaagcgcg gcgcggatgt
     1321 aggagagggc ggggtcgcgg cccgggaggg cctcgacgat gcacgtgacc tcggggtcgt
     1381 tcagcacggc ctcgaacgag tcggtcatca gggggccgtc cgcgtggccg ggtcgccgca
     1441 gaatgtgcct gatcctgatc ccgcagtcac gctcgtccag aagttcggcc acggtgcggc
     1501 cgacggttcc gtatccaagc agtccgatgt tcaattgggg ctccgttttc gtgaagggcg
     1561 attcgctcac gcttcaggat tgaagcctga gcggaaaaca ccgcttatgc atcatatcgg
     1621 attattctgc caacgtcttc gggcgcatgt cttaggtgaa atgccctaga aggctttagc
     1681 cttgaggtga aacgcggttt ttttgcaggc tttgtgccac aatgaaccga ttgttcccaa
     1741 atttcggaga agttcctcca tgatctacca cagcacgaga agccttgaaa acggcgtgaa
     1801 tgcccgcgaa gccctgctca agggcctgtg cgacgacggt ggcctctatg tgagcgacga
     1861 gctcctcacc tgcgtcctga cgcccgacat gctcaagggc ctcacgtacc gcgagacggc
     1921 gctgcgcgtc ttctcggtcc tgctctccga cttcacgaag accgagctcg ccgagggcat
     1981 tgagcgcgcc tatgccgaca acttcgcgag cgtcgccgtg acgcccgtga cccgcgtggg
     2041 cgacgaattc ctgctcgagc tgcatcacgg tcccacgtcc gccttcaagg acgtcgccct
     2101 gtgcatgctg ccgcagctca tgagcgccgc gctcaaggac acgggccgcc gcgtcatgat
     2161 cgcgacggcg acctcgggcg acacgggcaa ggccgccctt tcgggcttca tgaacgtgcc
     2221 cggcatcggc atcacggtct tctatccgca tggcaaggtg agcgacatcc agtacctgca
     2281 gatggcgacc caggagggcc gcaacgtcgc ggtcgccgcc gtcgagggca acttcgacga
     2341 cgtgcagagc accgtcaaga agatcttcgc ctcggacctc aggcaggagc tcgccgacga
     2401 aggcgtggaa ctctcctcgg ccaactcgat caacatcggc cgcctcgtgc cccaggtcgt
     2461 ctactacttc gacgcctacc gccagctcgt cgaggccaac gaggtcgaac agggcgccaa
     2521 ggtcgacttc tgcgtcccga cgggcaattt cggcgacgtg ctcgcgggct actatgcgta
     2581 ccgcatgggc ctgcccgtgc gccacttcat cgtggcgagc aacgccaaca acgtgctcac
     2641 ggacttcctg acgacgggcg tctacgaccg caaccgtccg ttcgtgaaga cgatcacgcc
     2701 gagcatggac atcctgatct cgagcaacct cgagcgcatg ctctactacg catccgaggg
     2761 cgacacggcg ctcatcgccc gcctcatgga gaacctcagg aacgagggtg tcttcagggt
     2821 cgagggcgag atgctcgagc gcatccgctc gctcttcaag tgcggctggg ccgacgacgc
     2881 cgaaacctcc gcgatgatcc gcgaggcctg gcgcaaggac ggccgcctcg tcgacccgca
     2941 caccgccgtg gccctcaagg tcgcccgcga gcgcgccgac aggtcggtgc cgtgcgtggt
     3001 gctctcgacg gcgagcccct tcaagttctg ccgcgacgtc tacatcgcgc tcactggcag
     3061 gccgcttgaa ggcgatcccg acggcttcgc atacatggac gcgctttccg ggctcacgag
     3121 tgaaaacgcc cccgcgcctc ttgcagggct gcgctcgatg cccgtgctcc acaaggccgt
     3181 cgtgcgtccc gagggcatgg ccgacttcat ccgcgcggcc gtcgcgcgcg cattctgaca
     3241 ggggaggccg cacatgaaag tcaccgtccg cgttcccgcc tcgacctcga acctcggtcc
     3301 cggctacgat tcgctcggca tggccctctc gctgcaggcg aagttcacgt tcgagccctc
     3361 cgacgagctc gtgatcgagg gctgtcccga ggcctaccga aatgccgaca acctcgtcat
     3421 gcagggcttc cgcatggtct gcgagaaggc gggccgtccc gtgcccgccg tgcatctcgt
     3481 gatcgactcc gaggtgcccg tcgctcgcgg tctcggatct tcgagcacct gcattgcggg
     3541 cggcatggcc gccgccaacg cgatgctcgg caatccgttc acgaccgacg acctcttcca
     3601 actctgcacg gccttcgagg gccatcccga caacgccgcg ccggccttcc tcggcggcct
     3661 gacggcgagc ttcatgcacg agggcagggc cgtcgccgtt ccgttctcgc ccgatcccgc
     3721 ctggcgcttc gtggccgtga tcccgaacta cgaggtccgc accgaggagg cccgcaggat
     3781 cgtcaggcgc gacatcgacc tctcggccgc cgttcacacg atgtcccacg ccatcgcgat
     3841 ggtgaggggc cttgagacgg gcgacgaggc gctggtgggc gctgcctgcg cggacgtcct
     3901 gcatgagccc taccgccgca cgctcatcgc cgagtacgac gccatgcgcg ccatggcgct
     3961 cgaggcgggc gccgccacct tcttcatcag cggctcgggc tcgaccatga tcgctgccac
     4021 gaagtccgag gccgtcgccg aagccttcgt cgcgaaggtc cgcgagacct ggcccgactt
     4081 cgccgccaga gtgctcaccg tctgtcacga gggcacgact gtcacggtcg aggactgagg
     4141 ctgcagcttc tgaaggaaca aaaaggcgcc gtctcccgaa tgaggagtcg gcgccttttg
     4201 cttgggcgtt ctgtttgcgg acgacgggat ccgggatcaa tgttcgagca cgtgcggatt
     4261 ctcgagaaga ctttcgggcg taccgtagcg cacgaggcag atctcggcgc cgccgatctg
     4321 gaggatgagg ccgagcggag cgctctctac gtcgaggacc gcatagaggc tgtcgccgtt
     4381 tccgacgggt cgcagcccct cgggtacgcg cctgttcgtg agctcggtga agagcgcctc
     4441 aagggcggcg atgatggcgg aacgcctcgg gtcgcccacg atgagggact tgtcgaaccg
     4501 gaactgcttg gtttcgagaa gctcgagcgg cgcgtcgggg ccgctctgaa gcatcgagcc
     4561 gtcggcgctt tcgcgctcgc gttcacgcca gcgcaacttg gcctgcgcgt cctgcagggc
     4621 gcgcagcgtg acgagcgagg gcagaagcgt ctcgggcttg gccgcagggg gattgaacga
     4681 gtagaagggc tctgcgggat cgggacgggc gaagaggtcg ccctgcacga agtgcgcgcc
     4741 tgcgtcccca ccgtccgtcc tcttcggacg ggcacgtccc ttgcgcagcg gcacgacacc
     4801 ttcgcgtgca gtgcgctcga gaagctggcg gatcacgccc tcgggtgtca gtccggcccc
     4861 tgcgagcact tcggccgcct tccccgcctc gatctcgtcg agcgtgatgg tgaacgtgag
     4921 ggtcttctgc atgggcttgg gaataagtga atcatgcggg cggaacgctg tgtccgcctt
     4981 gtctgtctga gtctagcaga ggaaatttgg aaaaacgctc aaaaccatgt tttccctcag
     5041 gcgcaggtgc cggaatgttg cgcggacgca tccaaaggaa gccctctcac gggacggcac
     5101 ggc
//
LOCUS       DBMDTI010000137         5087 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3969_length_5087_cov_2.972924, whole genome shotgun sequence.
ACCESSION   DBMDTI010000137 DBMDTI010000000
VERSION     DBMDTI010000137.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3969_length_5087_cov_2.972924"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="ACZPB6_07025"
     CDS             complement(<1..641)
                     /locus_tag="ACZPB6_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR1184254.1"
                     /translation="MTDTNTQELAKSFDPAAIEARWYPIWEERGYFKAGLDPKKPSFS
                     IQLPPPNITGILHMGHAFNQTVMDTLTRYHRMAGYNTVWIPGTDHAGIATQIVVERKL
                     EKQGIDYKSLTREQFIEKVWEWQKFSGGTILNQMRRIGDSVDWDRTYFTMDEKLSKVV
                     IETFVRLYEEGLIYRGNRLVNWDPKLQSAVSDLEVESAEENGHMWEIRYPAADG"
     gene            complement(803..1204)
                     /locus_tag="ACZPB6_07030"
     CDS             complement(803..1204)
                     /locus_tag="ACZPB6_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184255.1"
                     /translation="MERKEPVIDSRAVDLTDRVKLTWRDVRTLADEAAASTQPQIRAD
                     EPPAPEVLTAETATSSARLTPDERAALAAQLAPAIEAAVKHALRETLDLALQNALARV
                     RGDVERSVNGLVAEAVSQELERLDLDKIARR"
     gene            complement(1234..1662)
                     /locus_tag="ACZPB6_07035"
     CDS             complement(1234..1662)
                     /locus_tag="ACZPB6_07035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HIR1184256.1"
                     /translation="MQKIDFHYNVPDRLRYACLVARTVYRRGLTLAVWTADERRLREL
                     DSLLWRFDDLAFLPHVRAGSPLAGETPIRLSSRLEELEGDVLLLLDDQLPPEWQERLD
                     RFSRIIDVVSTKPEELQASRSRFRAYKAAGVELAAYDRRS"
     gene            complement(1705..3210)
                     /locus_tag="ACZPB6_07040"
     CDS             complement(1705..3210)
                     /locus_tag="ACZPB6_07040"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR1184257.1"
                     /translation="MTPALRFLSTAITTASPDEASSSLFIAGVFLNGGKPRLTPSAAE
                     FDEARGGTLTRMIECGAFDAKAGSTLFLGETEAFPNGFAVLGLGEEKDFSRKVFAECA
                     AKAARAFAWAESQAYTVQEWLPPKTSGKSSARLFSTAVLNALTPRCTLKTEHDDGIRT
                     KRIFWIDDLVTDKLLQGLDEGGVTAEAMSISRGLADLPGNVCTPAYLTEAVQNAAAGM
                     TTVEVEVLDDHQIEELGMGAFLSVAKGSTVPPRFIAIRYRGTNAVLPPVALVGKGITF
                     DAGGLSLKPAANMADMTYDMSGAAVVIGTVMAAARAKLPVNLLGVVAACENLPSGSAA
                     KPGDVVTSLSGKTIEILNTDCEGRMILADALTWTVRQKPELIIDAATLTGACCVALGA
                     PYSGLFTSDDALAYDLIKAGRDSLDDVWRLPVGAEYAKLMKTPAADIANQATVRDGGA
                     SSAAAFLSVFAEDVRWAHLDIAATANTSGRDRHSTGRPVPMLMRFLLKKMR"
     gene            3340..3738
                     /locus_tag="ACZPB6_07045"
     CDS             3340..3738
                     /locus_tag="ACZPB6_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013098.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1184258.1"
                     /translation="MDAQPKLRLSRPTNHLEAILSFYVDGLGFEVLDRFDNQQGWDGV
                     ILGHRDWPYQFEFTARRDESVVPPAPTPEHFLVFCIPEGEYWQKRLKALFEAGFQQVT
                     PPQLYADEGTATYEDPDGYRVVLRCGRWKK"
     gene            3837..3912
                     /locus_tag="ACZPB6_07050"
     tRNA            3837..3912
                     /locus_tag="ACZPB6_07050"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3870..3872,aa:Ala,seq:cgc)
     gene            4045..4602
                     /gene="greB"
                     /locus_tag="ACZPB6_07055"
     CDS             4045..4602
                     /gene="greB"
                     /locus_tag="ACZPB6_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HIR1184259.1"
                     /translation="MADENDDDEVRLPGLPPNTKNYMTPACYRRLLDEREHLVNVERP
                     EVVNVVSWAASNGDRSENGDYLYGKRRLREIDRRIRFLNKRIESAEVVDPDSRPPTDK
                     IFFGATVLYCDQKGEEHKIRIVGIDEADANHGDVSWISPIARVFLKAREGDEVKLPTP
                     ATVTHPAGVETLEILEVTYTSRQPR"
     gene            complement(4677..>5087)
                     /locus_tag="ACZPB6_07060"
     CDS             complement(4677..>5087)
                     /locus_tag="ACZPB6_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1184260.1"
                     /translation="KIVGFSRAGHGLVIHRADCPHVERGRKAEPSRWVDVQWAPDQRN
                     ANFAVPLEIRVEDPHASIAQVATSVAEANSSIVGMMIDDEDAQKFIRITIQVRDRGHL
                     MHVMRTIRQNANVEQVKRVFEAGIPSDRRRQARR"
BASE COUNT          967 a         1430 c         1695 g          995 t
ORIGIN      
        1 ccgtcggcgg caggatagcg gatctcccac atgtggccgt tttcttcggc gctttcgact
       61 tcaaggtcgg agacggcgga ctgaagcttc gggtcccagt tgacgaggcg gttgccgcgg
      121 tagatgaggc cttcctcata aagacgaacg aaggtttcaa tcacgacctt ggagagcttt
      181 tcgtccatcg tgaagtaggt gcggtcccaa tcgacgctgt cgccgatgcg gcgcatctgg
      241 ttgagaatcg tgccgcccga gaacttctgc cattcccaga ctttttcaat gaactgttcg
      301 cgcgtcaggg acttgtagtc aatgccctgc ttttcaagct tgcgctcgac gacgatctgc
      361 gtggcgatgc cggcatggtc cgtgcccgga atccagacgg tgttgtagcc ggccatgcgg
      421 tgatagcgcg taagcgtatc catgaccgtc tgattgaagg catgtcccat gtgcagaatg
      481 cccgtgatgt tgggcggcgg aagctgaatg gaaaagctcg gcttcttcgg gtcgagaccg
      541 gccttgaagt agccgcgttc ctcccagatc gggtaccaac gcgcttcgat ggcggccgga
      601 tcaaagctct tggcgagctc ctgagtgttc gtatcagtca ttgttagcca gtaaaaggac
      661 gatgaggagt ctgcaggagg cgagcttcgg cctgagccga gacgcttggg cagacagtat
      721 gaaaattctg ttattgtcgc acgaattgcg gggtgaaaag aaagcccgcc gaagtttttc
      781 aggcgggccg tggtgcgccg gatcagcggc gggcgatctt gtcgaggtca agtcgctcga
      841 gttcttggga aacggcctcg gcgaccagtc cgttcacgga gcgctcgaca tcccctctga
      901 cgcgtgcgag tgcgttctga agcgccagat cgagcgtttc gcgcagggca tgcttcacgg
      961 cggcttcaat ggcaggagcg agctgtgcgg cgagggccgc tcgctcatcc ggcgtgagcc
     1021 gggcggaaga tgtggcggtc tcggcggtga gcacttctgg cgcaggcggt tcgtccgccc
     1081 tgatctgagg ctgcgtggaa gcggccgctt cgtcggccag cgtcctgacg tcgcgccagg
     1141 tgagcttcac gcggtccgtg aggtcgacgg cacgggagtc gatgacgggt tccttgcgtt
     1201 ccatgggcat gggtctccaa tgaaaaagtc gtctcagctt cggcggtcgt atgctgcaag
     1261 ctcgacgcct gcggccttgt acgcgcggaa gcggctgcgg gaggcctgaa gttcctcggg
     1321 tttcgtgctt accacgtcga tgatgcggct gaagcggtcg aggcgctcct gccactcggg
     1381 aggaagctga tcgtccagaa gcagaagcac gtcgccctca agttcctcaa gacggctgga
     1441 aagcctgatg ggcgtctcgc ctgcgagagg actgcctgcg cgcacgtgag gcaggaatgc
     1501 cagatcgtca aagcgccaga ggagcgagtc aagctcgcgg agcctgcgct cgtccgccgt
     1561 ccagacggca agcgtgagcc cgcgccggta aacggtgcgg gcgacgagac aggcgtagcg
     1621 gagccggtcg gggacgttgt agtgaaagtc gatcttctgc atgtctgtca ttatggccga
     1681 tgagaagctc ccgaggtgcg atgatcagcg catcttcttg agaaggaagc gcatgagcat
     1741 cggcacgggg cgccccgtcg aatgcctgtc tcggcccgac gtgttggccg tcgccgcgat
     1801 gtcgagatgc gcccagcgga cgtcctccgc aaagacggag agaaaggccg cagcactgga
     1861 ggcgccgccg tcgcgcaccg tcgcctgatt ggcgatgtcg gcggcgggcg tcttcatcag
     1921 cttggcatac tcggctccga cgggcaggcg ccagacgtcg tcgagcgagt cgcggcctgc
     1981 cttgatgagg tcgtacgcga gcgcatcgtc ggatgtgaag aggccgctgt agggggcgcc
     2041 gagcgcaacg cagcaggctc ccgtcagggt ggccgcatcg atgatgagct cgggcttctg
     2101 acgcacggtc cacgtcaggg cgtctgcgag aatcatgcgg ccctcgcagt cggtgttgag
     2161 aatctcgatc gtcttgcccg agagggaggt gacgacgtcg cccggtttgg cggcgctgcc
     2221 cgaaggaagg ttctcgcagg ccgccacgac gccgagaagg ttgacgggca acttggcacg
     2281 ggcggcggcc atcacggtgc cgatcacgac ggccgccccc gacatgtcat aggtcatgtc
     2341 ggccatgttg gctgcaggct tgagcgaaag gccacccgcg tcgaacgtga tgcccttgcc
     2401 gacgagcgcg acgggcggaa ggacggcgtt ggtgccgcgg tagcggatgg caatgaagcg
     2461 gggcggaacg gtgcttccct tggcgacgga aaggaaagct cccatgccga gctcctcgat
     2521 ctgatgatcg tcaagaacct cgacttcgac ggtcgtcatg cctgcggcag cgttttgaac
     2581 ggcttcggtc agatatgcgg gcgtgcagac gttgcccggc agatccgcaa gaccacggga
     2641 gatcgacatg gcctctgccg tcacaccccc ttcgtccaac ccctgaagaa gcttgtcggt
     2701 gaccaaatca tcgatccaga agatgcgctt ggtgcggatg ccgtcatcgt gttccgtctt
     2761 gagcgtgcaa cggggcgtga gcgcattgag gacggcagtg gagaaaagac gtgcggaaga
     2821 tttgccgctc gtcttcgggg gaagccattc ctgaacggtg taggcctgag attccgccca
     2881 cgcgaacgcg cgggcggcct tggctgcgca ttcggcaaac actttgcggg agaagtcctt
     2941 ttcctcgcca agaccgagaa cggcaaagcc gttggggaag gcttccgttt cgccgaggaa
     3001 gagcgtgctg ccagccttgg cgtcgaaggc gccgcattcg atcatgcgtg tgagagtgcc
     3061 tccgcgtgct tcatcaaatt cagcggcgga aggcgtcaga cgaggcttgc cgccgttcag
     3121 aaagacgccg gcgatgaaga gggagctcga ggcttcatcg ggcgaggccg tcgtgatggc
     3181 ggtggaaaga aatcgaagag cgggagtcat gcgtgtttcc tgaagactgc gggatggccg
     3241 taaggccgac aatattccat tctaactatc cctagggtca atggcatttc aatccgccct
     3301 gtccgataat gaagaagttc agcttttagg agtcttctca tggatgcaca gccgaaactc
     3361 agactttccc gtccgaccaa ccatcttgaa gccattctgt cgttctacgt cgacgggctc
     3421 ggctttgaag ttctcgaccg ctttgacaat cagcagggat gggacggcgt gattctcggt
     3481 caccgagact ggccctacca gttcgaattc acggcccgtc gcgacgagtc cgtggtgccg
     3541 cctgcaccga cgcccgagca cttcctggtc ttctgcattc ccgagggcga atattggcag
     3601 aagcgtctca aggcgctctt tgaggcgggc ttccagcagg tgacgcctcc ccagctctat
     3661 gccgacgaag gcacagccac ttacgaggac cctgacggct atcgagtggt actccgttgc
     3721 ggccgatgga aaaaataagg gtaccgtatt cgcggtatac caagccggac ggaaacggtg
     3781 cataatgcaa tgcctgtgca tcgcacgtcg aagcataggt atccaaaagg ttccaagggg
     3841 gggtagctca gctgggagag cgctgcgttc gcaatgcaga ggtcgggagt tcgatcctcc
     3901 tcctctccac caaagacctt tcaagaagtc ccgtacggtt ttgccgtagc gggatttttt
     3961 ttcgcgcatg gattgcactc cgccttttcg agcctgcgta gtgacgctgg gaattcggga
     4021 gcggagcttt ttcggagaga tgacatggct gatgaaaacg atgacgacga ggtcaggctt
     4081 cccggactgc cgcccaacac gaagaactac atgacgcccg cctgctacag gagacttctc
     4141 gacgagcgtg agcatctggt caatgtcgag cgtcccgagg tcgtcaatgt cgtgtcctgg
     4201 gcggcgagca acggcgacag aagtgaaaac ggtgactatc tgtacggcaa gcgccgtctg
     4261 cgtgaaatcg acaggcgcat tcgcttcctc aacaagcgca tcgagtcggc cgaggtcgtg
     4321 gatcccgact cccgtccccc tacggacaag attttcttcg gggcgacagt gctctactgc
     4381 gatcagaagg gcgaggagca caagatccga atcgtcggta tcgatgaggc cgatgccaat
     4441 cacggtgacg tgagctggat cagtccgatc gcccgcgttt tcctcaaggc tcgcgagggc
     4501 gacgaagtga agttgccgac gccggcaacc gtgacgcacc ctgcaggcgt ggagacgctg
     4561 gagattcttg aggtgacgta tacgagcagg cagcctcgct gaggcgtgtt ggtgatgaac
     4621 cagaaggccg gagcagcaat gctctggcct tctggttcat ggggaggcgc gggaggtcag
     4681 cgtcgtgcct gacggcggcg gtccgatgga atgcccgcct cgaagacgcg cttgacctgc
     4741 tcgacgttgg cgttctggcg gatcgtgcgc atcacgtgca tcagatgacc gcggtcgcgg
     4801 acctgaatgg tgatgcggat gaacttctga gcgtcctcgt cgtcgatcat catgccgacg
     4861 atgctggagt tggcttcagc gacgctcgtc gcaacctgag cgatcgaagc gtgaggatct
     4921 tcgacgcgga tttcgagcgg tacggcgaag ttggcgttgc gctgatcggg cgcccactgg
     4981 acgtctaccc agcgtgacgg ttcggccttg cggccgcgct cgacgtgcgg gcagtccgcg
     5041 cggtggatga cgaggccgtg accggcgcgg ctgaagccca cgatctt
//
LOCUS       DBMDTI010000138         5080 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_3976_length_5080_cov_1.820044, whole genome shotgun sequence.
ACCESSION   DBMDTI010000138 DBMDTI010000000
VERSION     DBMDTI010000138.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3976_length_5080_cov_1.820044"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            498..839
                     /locus_tag="ACZPB6_07065"
     CDS             498..839
                     /locus_tag="ACZPB6_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024908.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1184261.1"
                     /translation="MTEFCSIDRVTYPLPCQQSYMRWSAHSLPPLDTVSQGRRESGLQ
                     QMHPEALQNVRKRAFEMLDAGMNCVEIARKLSAHPQTIRKWKKHRETFGEELSVIGLK
                     RGPKKLRPEVL"
     gene            1027..1725
                     /locus_tag="ACZPB6_07070"
     CDS             1027..1725
                     /locus_tag="ACZPB6_07070"
                     /inference="COORDINATES: protein motif:HMM:NF007621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HIR1184262.1"
                     /translation="MSEEDKREAGSPARGGARTGAGRPKGSGTYSEVRSVRLRPDEAE
                     HCRRKGGNRYLRGLIRADMTREEAPEGEDRPADVLPREHLNVRVVGRVPLETDPKDNV
                     TRVEMKGSCGFPSPADDYASEEFNLNDFFVRKPHTTFVIEADGDSMIDAGISSGDILL
                     VDSSKEPVDGDIVLAYLGGALTIKRFKRIDGVIELRPENKEGNYRILRPTEWDDFRVA
                     GVVTALGRILGRGP"
     gene            1757..3055
                     /locus_tag="ACZPB6_07075"
     CDS             1757..3055
                     /locus_tag="ACZPB6_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013015.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0034061 - DNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR1184263.1"
                     /translation="MTTRFLHIDGNTFYASVHRVFDPTLRRRPVVVLSNNDGCVVTRT
                     AEAKALGIARGVPYFQIRELAERHGVAVLSSNYELYQSMSDRMMAVIRTLVPRQEIYS
                     IDEQFADVSGMENLTALGREARARVLKWTGIPTCAGIAPTKTLAKLCDHWAKVHSVFG
                     GVLNWDDLSPARREKAFAVTPVSEVWGVGGRTAAKLEALGVRTVLDFVRVPGGVVRRL
                     GGVVLERTWLELQGRCCIELEETPPGRKQIVRSRSLSAPVSDLDGLLCAASVRMESAA
                     RVLRREGSEATHVGVLFSTSRFRENEPQHSAEVGERLLRPTADTIELTAVAEALVRRA
                     WRRGFAYCRLGVVLSGLVPAVDGAARCVQPQSLFDEPEDDSARERRRRLMMTADMITG
                     RFGRDAIRTAACLQADGWEMRRDRMTPCSTTCWDEMPGVD"
     gene            complement(3266..3877)
                     /locus_tag="ACZPB6_07080"
     CDS             complement(3266..3877)
                     /locus_tag="ACZPB6_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012920.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HIR1184264.1"
                     /translation="MNSTSTDTLGGRIGSELKRSGISMRELARRIDIAQPSISKWCSN
                     QAVPRVIYLERMAKVFGVSAHWLTYGEHASGRDEVVIRPRPESSETIGLSLTADSAKR
                     TFGTEANKLSLFTQESDDMAPTIRSGSLVVINHTINKVIGSGVYLVQVGETKELRRIQ
                     PLVDGTLDIRIDNPKRFANEIATDDKIVILGKVLSTVSVKHIR"
     gene            complement(4465..>5080)
                     /locus_tag="ACZPB6_07085"
     CDS             complement(4465..>5080)
                     /locus_tag="ACZPB6_07085"
                     /inference="COORDINATES: protein motif:HMM:NF019843.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1184265.1"
                     /translation="LLAEGRIYSADLPAAKELLSRGCTIGDDRSCREMARIANDAGEH
                     EALVSWLGKSCMLGNETSCMRTATLDRSDDRVRWVEEACRLGRMDACLDAAERRQNGN
                     GVPVDMKLALQNYQAACRLSNAPSCFRAAKILRSGDGVKADPKAAAVWYDKACQMRQT
                     RACAILGDLYLSGEGVDKDDVRARFYHNIACSSGLRSSCPKAGR"
BASE COUNT          955 a         1403 c         1775 g          947 t
ORIGIN      
        1 cggagggctt ttcggcgatg aagagcttca tgagggatcc gttcgagatt atcggagcag
       61 tgtacagatt tcgcgctccg gacgcacggc ccgctcaaaa aagccttcaa tttcgactta
      121 cgattgtatg gaaaagcaac ggacttgatc cagccgatgt ttatagacaa tctttacact
      181 tgtcgtttgc ttttgtcgaa cattaagttc gaggtattcg agcgttctgc caattgaaaa
      241 aaacaatcgc taaaaactag ggaaaaatac gcaagtcgaa ggtcgagccc cgcgtggttt
      301 ttcttgagct tcgtcaaggt cgggaaaacc ctccgaatgt cgcgtcggaa aaagcgaagg
      361 gatgttgggt aaaaaccctg agtctaggtg cgacttttga gcaactgccc cctgtgggag
      421 agggacgtcg gaggcgttca cgtgttagca tcgaatccgt ccccccgaca agggggcttg
      481 acaaacagga ggcaaacatg actgaattct gctcgattga ccgggtgacc tacccgctgc
      541 cgtgccagca gtcctacatg cggtggagtg ctcattcgct ccccccgctt gacaccgtgt
      601 cgcagggtcg gcgcgaaagc ggcctgcagc agatgcatcc cgaagcgctt cagaacgttc
      661 gcaagcgcgc attcgagatg cttgacgccg gcatgaactg cgttgagatc gcaagaaagc
      721 tcagcgcgca cccgcagacg atccgcaagt ggaagaagca tcgcgagacc ttcggtgaag
      781 agctctcggt gatcggcctc aagcggggcc cgaagaagct ccgtccggag gtgctctaaa
      841 aagagacgca gtcactgcga ctgcaaggcg gacggcccgc aggattgaga ggaatcctgc
      901 gggccgtccg cttttttgag attcgcaaat ggtgatgcgg aacggggttt tcgggtgacg
      961 cggcttttaa tttgattttt gagctacaat tccggcattg gatgaggttg tctgaaggag
     1021 gccgggatgt cggaagagga caagagggaa gcgggttcgc ccgcgagggg cggtgcgcgc
     1081 acgggcgcgg gccgtccgaa gggatcggga acctattccg aggtgagaag cgtgaggctc
     1141 aggcccgatg aggccgagca ctgcaggcga aagggcggca accgctacct gaggggcctc
     1201 attcgtgcgg acatgacgcg ggaggaggcg cccgaagggg aggaccggcc tgcggacgtg
     1261 ctgcctcgcg agcacctcaa cgtacgcgtc gtcgggcgcg tgccgcttga aaccgatccg
     1321 aaggacaacg tcacgcgcgt cgagatgaag ggctcgtgcg gctttccgtc tccggcggac
     1381 gactatgcga gcgaggagtt caacctgaac gatttcttcg tgcgcaagcc ccacacgacc
     1441 ttcgtgatcg aggcggacgg cgactcgatg atcgacgcgg ggatctcctc gggcgacatt
     1501 ctgctcgtcg acagctcgaa ggagcccgtc gacggcgaca tcgtgctcgc ctatctcggc
     1561 ggcgcgctca cgatcaagcg cttcaagcgc attgacggcg tcattgagct gaggcccgag
     1621 aacaaggagg gcaactacag gatcctgcgt cccaccgagt gggacgactt cagggtggct
     1681 ggcgtcgtga cggcgctcgg ccgcattctc gggcgcggcc cctaggccgt gcgcctgtcg
     1741 acgggaggag tcctgcatga cgacgcgctt tcttcacatt gacggcaaca ccttctatgc
     1801 ctcggtgcac agggtgttcg atcccacgct caggcgccgc cccgtggtgg tcctctcgaa
     1861 caacgacggc tgcgtcgtga cgaggacggc tgaggcgaag gcgctcggca ttgcgcgggg
     1921 cgtcccgtac tttcagatca gggagctcgc cgagcggcac ggcgtcgcgg tgctctcgag
     1981 caactacgag ctctatcagt cgatgtcgga ccgcatgatg gcggtgatcc gcacgctcgt
     2041 gccgaggcag gagatctatt cgatcgacga gcagtttgcg gacgtctcgg gcatggagaa
     2101 tcttacggcg ctcggccgcg aggcgagggc tcgcgtgctc aagtggacgg ggattccgac
     2161 ctgtgcgggc attgcgccca cgaagacgct cgccaagctc tgcgaccact gggccaaggt
     2221 tcattccgtc ttcgggggcg tcctcaactg ggacgacctt tcgcccgcgc gcagggagaa
     2281 ggcctttgcg gtgacgcccg tctcggaggt ctggggcgtg ggaggcagga cggccgccaa
     2341 gctcgaggcc ctcggcgtgc ggacggtgct cgacttcgtg agggtgccgg gcggcgtcgt
     2401 gaggcgcctg ggcggcgtgg tgctcgagcg aacctggctc gaactccagg ggcgctgctg
     2461 catcgagctt gaggagacgc cgccggggag aaagcagatc gtgcgctccc gcagcctctc
     2521 ggcgcccgtc tccgacctcg acggcctgct gtgcgcggcc tccgtgcgca tggagagcgc
     2581 ggcccgcgtg cttcggcgcg agggaagcga ggcgacgcac gtgggcgttc tcttcagcac
     2641 gagccgcttc agggagaacg agccgcagca ttcggccgag gtgggcgaaa ggctcctgcg
     2701 gccgacagcc gacaccatcg agctcacggc ggtcgccgag gcgctcgtga gaagggcctg
     2761 gcgaaggggc ttcgcctact gcaggctcgg cgtggtgctc tcgggactcg tgcccgccgt
     2821 cgacggcgca gcgcgatgcg tgcagccgca gtccctcttc gacgagcccg aggacgattc
     2881 ggcccgtgag cggcgcagaa ggctcatgat gaccgccgac atgatcacgg gacgcttcgg
     2941 gcgggatgcg atcaggacgg cggcctgcct gcaggcggac ggctgggaga tgcgcaggga
     3001 ccgcatgacg ccctgcagca cgacgtgctg ggacgagatg ccgggcgtcg actgaggacg
     3061 aaatgcatgc ccggcctccg aaggaaggac gtagcacgat tcgtggtcat ggatagtgtt
     3121 tgtgtgtgaa acagggtgct tcgacatttc cccactcgtt cgagccggcc ggttatgtcc
     3181 tcatcggacc ggcgctcgat ggcgatctga tcggagacaa agaaaaaggg cctccggaag
     3241 ggaggccctt tgttctgatc gctgcttagc ggatgtgctt cacggaaacc gtggagagca
     3301 ccttgccgag aatgacgatc ttgtcgtcgg tggcgatttc gttcgcaaag cgcttgggat
     3361 tgtcgatgcg gatgtcgagc gtgccgtcaa cgagcggctg gatgcggcga agttccttcg
     3421 tttcgccgac ctggacgagg tagacgccgg agccgatcac cttgttgatc gtatggttga
     3481 tgaccacgag gctgccgctg cggatcgtcg gagccatgtc gtcggattcc tgcgtgaaga
     3541 gcgagagctt gttggcttcc gtgccgaagg tgcgcttggc gctgtcggcc gtgagcgaca
     3601 ggccgatcgt ttcggaggat tcgggacgcg ggcggatgac gacttcgtca cggccggagg
     3661 cgtgctcgcc gtaggtgagc cagtgagcgg aaacgccgaa gaccttggcc atgcgctcaa
     3721 gatagatgac gcgcggaacg gcctggttgg agcaccactt ggaaatggaa ggctgggcga
     3781 tgtcgatgcg gcgggcgagc tcgcgcatgg agatgccgga acgcttgagt tcggagccaa
     3841 tgcggccgcc gagtgtgtcg gtcgaggtgg aattcacgat agtccccttg tgaatgtgta
     3901 atgagatcag ctggtttttc ggatatcgac ggcttttgtg ggtccgttga tatcacttgt
     3961 tgcttataaa atacctcaaa cagctgaatc gcgattcgga gtatgcactt aggtgaacgg
     4021 gaatcggcga tactttgcgt caaaatcctg ttcccgatgc ggattcattt tacacagagt
     4081 gagagagtcg aactaagaaa acgctatcgt cgccgcaaga cgcacggaac cttgcgcaaa
     4141 ccgcaattgg ctaatctccc cagtcgcaga ggcctttaca cggatctatg gggtaattcg
     4201 gaaatgaacg gttacaaact ttgggtgtgt gtggacggaa attgacgggt gatccggtcg
     4261 agaatagggg aagtcgggaa tgactgcggt ccgggcgggg cgggggaagg gagtacgtcg
     4321 tctgcgggga gggccggagg tggtctccgg ggggctgatc ctgaaggtat cagcgggagt
     4381 gtattcgctg tgaacgccag tgggactagg tccttgagtg gattttgtcc caccggaagt
     4441 tcgattcggg ctcggttggc gggctcagcg gccggccttg gggcagctcg agcgaaggcc
     4501 gctcgagcag gcaatgttgt ggtagaacct ggcgcggaca tcgtccttgt cgacgccctc
     4561 gcccgagagg tagaggtcgc cgaggatggc gcaggcgcgg gtctggcgca tctggcaggc
     4621 cttgtcgtac cagacggcgg cggccttcgg atcggccttg acgccgtcgc cagagcgcag
     4681 gatcttggcg gcgcggaagc acgacggggc gttgctcagg cggcaggcgg cctggtagtt
     4741 ctgaagggca agcttcatgt cgacgggcac gccgttgccg ttctggcggc gctcggccgc
     4801 gtcgaggcag gcgtccatgc ggccgagcct gcaggcctcc tcaacccagc gcacacggtc
     4861 gtcggagcgg tcgagggtgg cggtgcgcat gcaggacgtc tcgttgccga gcatgcagga
     4921 tttgccgagc caggagacca acgcctcgtg ctcgcccgcg tcgttggcga tgcgggccat
     4981 ttcacggcag ctgcggtcgt cgccgatcgt gcagccgcgc gagagaagtt cctttgcggc
     5041 cggaaggtcg gccgaataga tcctgccctc ggcaagcaga
//
LOCUS       DBMDTI010000139         5041 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4016_length_5041_cov_2.083198, whole genome shotgun sequence.
ACCESSION   DBMDTI010000139 DBMDTI010000000
VERSION     DBMDTI010000139.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4016_length_5041_cov_2.083198"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            54..1436
                     /locus_tag="ACZPB6_07090"
     CDS             54..1436
                     /locus_tag="ACZPB6_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1184266.1"
                     /translation="MRRKNKKIDMLNGSIWDKMIVFALPLAFTGMLQQLFNAADVAVL
                     GRFVSNESMAGVGNNVPVIGLIIAFCMGLALGANVVVARLLGMKEPERANRAVRTALA
                     TAVIFGIGAAIVGECFAGPAMDWLSVPEAVRDDALTYLRVYLLGMPFIAIYNFLAAVM
                     RSQGDTQTPLWALVAATLVNIAGNLFFVLAFGMGTAGVALATVLANALAASLLFFSLI
                     TADGPLHIEPREVFRIDMTALRPMVRIGWPAGLQGAVFSISNLVIQSAINSLGADAMA
                     GSVAAFTIEINIYCFINAFGLAATTFVSQNYGAKNLERCKRATYVATGLNMVATVLMV
                     ALVLVFGRTMLGLFTDVPAVVELAMARIFWVASFEPISVLMEVSSSAMRGYGYSMPPA
                     MATLICVCSVRLIWVWTVFAASPDYVTLMIVYPISWAVTVVPLLYLYVRLMKRIKRRF
                     EERALEAEAA"
     gene            complement(1508..4669)
                     /locus_tag="ACZPB6_07095"
     CDS             complement(1508..4669)
                     /locus_tag="ACZPB6_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024754.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR1184267.1"
                     /translation="MNFSPLQLRKPALAALGEQAENLFRDHHYRECTQVMRQFAEGVL
                     RTILKDDRHTYSEMMRTPLVRQLADSLTFEYFRRIRDLGNEASHFRLGTSGTMLETGE
                     RHYRVRTADDAAECLKYGHGIAVWLMSLLDREFRYPDFRPLTPPRARIAAQGAPVVFN
                     PDQQAAIELSQGRHLVFAPPGCGKTAILTERIAVALKDGVNLSDMLCLTFTNRAARGM
                     KERIDERFTDRCMDTLYVGNLHRFCSRLLFDNDVVSEISTILDEDDVHDVIEGLLIEN
                     IPGGCRGRSLPGNAADYIMARSARLFQEANGFPEETFHRTPEFDVNKKNQDELDAFGL
                     IKVDDFGRCIELDHAAIDAAARRYLEYKTAQHLLDFNDLLLKAWAWLDATPEACGRYA
                     WIQVDEVQDLSPLQLDIVKKLWNDSIDRSVCVYFGDEQQAIFSFMGAKLDTLRALHKT
                     HEIHRLYRNFRSPDYLLNMFNTYAQRVLECDPEFLPKAEKTVARPEGALRAIRTATPQ
                     SQLMLLCDLLEGRRPEETTAVIVPSNREADAVSAAFKAQDIEHFKISGSDIFRQNILE
                     AAKAYLSVLKDDFRSAPWAQLVYRLSSKKNLSLKKIRDYLAVEFPRRGLLASDLMLYS
                     THDEKEPVSALEDFMRAYEGELVVFDTETTGLDIGLDEVIQIAAVKLRAGEVVERLVL
                     YLETEREIPKMLGNIPNPMLEEYEAHRAELVTPEAGFKIFLDFVGYAPVVGHNVEYDA
                     NIVNAQYAVLKDQLRRVRGDAAGPDEDVDRTELVRRFDTLKLSHALEPAILKAAGLAR
                     LPSHKLKDLIAVLGLEGSNTHKADDDVEATVSLLRWFHAQARPLVELQRRFLSNPSVV
                     RMSDALRKIVLPMFLEHRNLMYRRMPAADEAILVQVFRQFIEALPNYTDDEGEIENIR
                     KKLPLIYEYLDRVLINTTSQPTLYAQLQRNLVQINSLKEPDLCSSDIVRDRVFISTVH
                     KAKGLEFDNVIVTSVIDGTYPFFKNHDKADILEDARKLYVAISRTKRTLVLTMPAANA
                     WNYAQRPSRFLESISTYFEKSSA"
BASE COUNT          812 a         1502 c         1718 g         1009 t
ORIGIN      
        1 ttgcacgtct tgaactggta aaatactatg tttccaaagg acaacgcaag gatatgcgcc
       61 gcaagaacaa gaagatcgac atgctcaacg gatcgatctg ggacaagatg atcgtcttcg
      121 ccctgcccct cgccttcaca ggcatgctcc agcagctctt caacgctgcc gacgttgcgg
      181 tgctcgggcg cttcgtctcg aacgagtcga tggcgggcgt gggcaacaac gtgcccgtca
      241 tcggcctcat cattgcgttc tgcatgggtc ttgcgctcgg cgccaacgtc gtggtggcga
      301 ggctgctcgg catgaaggag cccgagcggg cgaacagagc cgtgcgcacg gcgctcgcca
      361 cggcggtgat cttcggcata ggcgcggcga tcgtcggcga gtgcttcgcg gggcccgcca
      421 tggactggct gtcggtgccc gaggccgtga gagacgatgc gctcacctat ctgcgcgtct
      481 atcttctcgg catgcccttc attgcgatct acaacttcct cgccgctgtg atgcgaagcc
      541 agggcgacac gcagacgccg ctctgggcgt tggtggcggc gaccctcgtc aacattgcgg
      601 gcaatctctt cttcgtgctc gccttcggca tgggaacggc gggtgttgcg ctcgcgaccg
      661 tcctcgccaa cgcgctcgcc gcctcgctcc tcttcttcag cctcataacg gccgatggcc
      721 cgcttcacat tgagccgcgc gaggtgttcc ggatcgacat gacggcgctg cgcccgatgg
      781 tgaggatcgg ctggcccgcg ggccttcagg gcgcggtctt ctcgatctcg aacctcgtca
      841 tccagtccgc catcaacagt ctcggcgcgg acgccatggc gggttcggtc gcggccttca
      901 cgatcgagat caacatctac tgcttcatca acgccttcgg tctggccgcc acgaccttcg
      961 tgagccagaa ctacggcgcg aagaacctcg agcgctgcaa gcgcgcgacc tacgtcgcca
     1021 cgggcctcaa catggtcgcc accgtcctga tggtggcgct cgtgctcgtc ttcggacgca
     1081 cgatgctcgg actcttcacg gacgtgccgg ccgtcgtcga gctcgcgatg gcgcgcatct
     1141 tctgggtggc gagcttcgag cccatcagcg tgctcatgga ggtctcctcg agcgccatgc
     1201 gaggctacgg ctactcgatg ccgcccgcca tggcgaccct catctgcgtg tgctccgtgc
     1261 gcctcatctg ggtctggacc gtcttcgcgg caagccccga ctacgtgacc ctgatgatcg
     1321 tctatccgat cagctgggcc gttaccgtcg tgccgctgct ctacctctac gtgcgcctca
     1381 tgaagcgcat caagcgcagg ttcgaggagc gcgccctcga agccgaagcc gcctgacgtc
     1441 gtgtccgtca acgaaaacgg cccccgtgaa ggcaggggca gtttctgttc gagaggattt
     1501 ccagcggcta tgccgaggac ttctcgaagt aggttgagat gctctcgagg aagcgcgagg
     1561 gcctctgggc gtagttccag gcgttcgccg cgggcatcgt caggacgagc gtgcgctttg
     1621 ttcggctgat ggcgacgtag agcttgcggg cgtcctcgag gatgtcggcc ttgtcgtggt
     1681 tcttgaagaa cgggtacgtg ccgtcgatga cgctggtgac gatgacgttg tcgaactcga
     1741 gccccttggc cttgtggact gtggagatga agacgcggtc gcgcacgatg tcgctcgagc
     1801 agaggtcggg ctccttgagg gagttgatct gcacgaggtt tctctgcagc tgcgcgtaga
     1861 gcgtcggctg tgaggtcgtg ttgatgagca cgcggtcgag gtactcgtag atgagcggga
     1921 gcttcttgcg gatgttctcg atctcgccct cgtcgtccgt gtagttgggc agggcctcga
     1981 tgaactgcct gaagacctgg acgaggatcg cctcgtcggc ggcgggcatc ctgcggtaca
     2041 tgaggttcct gtgctcgagg aacatcggga gcacgatctt cctgagcgcg tcggacatgc
     2101 gcacgacgct cgggttggag aggaagcgcc gctggagctc gacgagcgga cgggcctggg
     2161 cgtggaacca gcgcagcagg ctgacggtcg cctcgacgtc gtcgtcggcc ttgtgggtgt
     2221 tgctgccctc gagtccgagg accgcgatga ggtccttgag cttgtgcgag ggaaggcgcg
     2281 caaggcccgc ggccttgagg atcgcgggct cgagcgcatg ggagagcttg agcgtgtcga
     2341 agcggcgcac gagctcggtg cggtcgacgt cctcgtcggg tcccgccgca tcgccgcgca
     2401 cgcgcctgag ctggtccttg aggacggcgt actgggcgtt gacgatgttg gcgtcgtatt
     2461 cgacgttgtg gcccacgacg ggcgcgtagc ccacgaagtc gaggaagatc ttgaagcccg
     2521 cctcgggcgt gacgagctcg gcgcggtgcg cctcgtactc ctcgagcatc ggattcggga
     2581 tgttgccgag catcttcgga atctcgcgct ccgtttcaag gtagagcacg agccgctcga
     2641 cgacttcgcc cgcacgcagc ttgacggcgg cgatctggat cacctcgtcg aggccgatgt
     2701 caaggcccgt ggtctccgtg tcgaagacga cgagctcgcc ctcgtaggcg cgcatgaagt
     2761 cctcgagcgc gctgacgggc tccttttcgt catgcgtgct gtagagcatg aggtcggagg
     2821 caagaagccc gcgcctggga aattcgacgg caaggtagtc tcggatcttc ttgagggaga
     2881 ggttcttctt cgagctgagc cgatagacga gctgggccca gggggcgctt ctgaagtcgt
     2941 ccttgagcac gctcaggtag gccttggcgg cctcgaggat gttctgcctg aagatgtcgc
     3001 tgccggagat cttgaagtgc tcgatgtcct gtgccttgaa ggcggcgctg acggcgtcgg
     3061 cctcgcggtt gctcggcacg atgacggccg tcgtctcctc ggggcgtcgc ccttcgagca
     3121 ggtcgcacag cagcatcagc tgcgactgcg gcgtggccgt gcggatggcg cgcagggccc
     3181 cttcgggtct tgcgaccgtc ttctcggcct tgggcaggaa ctcgggatcg cactcgagca
     3241 cgcgctgcgc ataggtgttg aacatgttga gcaggtagtc gggcgagcgg aagttcctgt
     3301 agaggcggtg gatctcgtgc gtcttgtgaa gggccctgag tgtgtcgagc ttggcgccca
     3361 tgaaggagaa gatcgcctgc tgctcgtcgc cgaagtagac gcacacgctc ctgtcgatcg
     3421 agtcgttcca gagctttttg acgatgtcga gctgcagggg cgagaggtcc tgcacctcgt
     3481 cgacctggat ccaggcgtag cgcccgcagg cctcgggggt ggcgtcgagc caggcccagg
     3541 ccttgaggag caggtcgttg aagtcgagca ggtgctgcgc ggtcttgtac tcgaggtagc
     3601 gccttgcggc cgcgtcgatc gcggcatggt cgagctcgat gcagcggccg aagtcgtcga
     3661 ccttgatcag tccgaaggcg tcgagctcgt cctggttctt cttgttgacg tcgaattctg
     3721 gcgtgcgatg aaacgtctcc tcggggaagc cgttcgcctc ctgaaagagc cttgccgagc
     3781 gcgccatgat gtagtcggcg gcgttgcccg gcagggaccg tcctctgcag ccgccgggga
     3841 tgttctcgat gagaaggccc tcgatcacgt cgtggacgtc gtcctcgtcg aggatcgtcg
     3901 agatctcgga gacgacgtcg ttgtcaaaga gaaggcggga gcagaagcgg tggaggttgc
     3961 ccacgtaaag ggtgtccatg caccggtccg tgaagcgctc gtcgatgcgc tccttcatgc
     4021 cgcgcgcggc tcggttcgtg aacgtgaggc agagcatgtc ggagaggttc acgccgtcct
     4081 tgagcgcgac ggcgatgcgc tcggtgagga tggcggtctt gccgcagccc ggcggtgcga
     4141 acacgagatg gcgcccctgc gagagctcga tcgcggcctg ctggtcgggg ttgaagacga
     4201 cgggggcgcc ctgcgcggcg atcctcgccc tgggcggcgt gagcggcctg aagtcgggat
     4261 agcggaactc gcggtcgaga agcgacatga gccagaccgc gatcccgtgt ccgtacttga
     4321 ggcactcggc ggcgtcgtcg gccgtgcgca cgcggtagtg gcgctcgccc gtctcgagca
     4381 tcgtcccgga cgtcccgagc ctgaagtgcg aggcctcgtt gcccagatcg cggatgcggc
     4441 ggaagtattc gaacgtgagg ctgtcggcga gctggcgcac gagcggcgtg cgcatcatct
     4501 ccgaataggt gtgcctgtcg tccttgagga tcgtgcgcag cacgccctcg gcaaactggc
     4561 gcatgacctg cgtgcactcg cggtagtgat ggtcgcgaaa gaggttttcg gcctgttcgc
     4621 cgagagcggc gagtgcaggc ttgcggagct gcagaggcga gaagttcatg ttgcgggtct
     4681 cctgcggcgg atgcggccgc aatcgaagtc ttgagtcctg accggatgtt tcgaacattg
     4741 tacgacggca tgccggaagg cacgtccctc aagcgggaag cgaaggggag gaaggcttcc
     4801 tgcctatatt tgcgtacctc ttgacgtcgc tcgagattcc tcggactttt ctcctaagac
     4861 atctcgagta gatgcgtcga aatcgctccg gaggctccca tagaatgatc ggacggccac
     4921 ttgaaaggct ctcttcatca attcaccaca ggaaacttct acatgcgttc tgatcccctc
     4981 aagaaaggca tcaacgcggc tccccagcgc gcgcttctta aggctctcgg tctctgcgat
     5041 g
//
LOCUS       DBMDTI010000140         4929 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4127_length_4929_cov_3.972038, whole genome shotgun sequence.
ACCESSION   DBMDTI010000140 DBMDTI010000000
VERSION     DBMDTI010000140.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4127_length_4929_cov_3.972038"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..4816
                     /gene="uvrA"
                     /locus_tag="ACZPB6_07100"
     CDS             <1..4816
                     /gene="uvrA"
                     /locus_tag="ACZPB6_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1184268.1"
                     /translation="KSFSQPSASLFSFNSPVGACEKCRGFGRIITVDPSLVIPDPSKT
                     LEEGAVKPWTTKSFNECQQEMLAYAAVRDIRTNTPYKKLTAEEKRWVWEGGEDWTGNW
                     RTQWYGINRFFEWLESKAYKMHVRMLLSRYRSYTECPSCRGSHLKQPSLFWRYGTLEE
                     KQLVLAKTIEGGVFKPIGMTLSDRAYAKLPGFNYHELMTLPIEDLRDFFKKRRARADE
                     SEQMLLDEICSRLTFLCDVGVGYLTLDRQSRTLSGGEVQRVSLTTALGTALVNTLFVL
                     DEPSIGLHPRDMHRVNRIMRRLTEAGNTLVVVEHDPQVMLAANRLIDMGPGSGTDGGR
                     IIFDGSTREVLDAGTLTGDYLSGRRRVRRPDPEFVTADRPRLIIKGASEHNLRHIDVA
                     IPMGAIVAIAGVSGSGKSTLVSDILVPALKRKSGKSTDQPGKFDGLAGCIPHDVIYVD
                     QSPIGRTTRGNPVSYVGSFDGIRSLFAATPMARHLGLSVGDFSFNSGKGRCPTCGGAG
                     FEHIEMQFLSDVYLPCPACGGKRYKDSTLEVKIRLSDDRDYSIADVLDMTADEATRLF
                     FEYPSITWGIQQLCKVGLGYLKLGQPLTTLSGGERQRLKLAGTLSEGLSLGRRCGYSA
                     GKLFVFDEPTTGLHFGDTATLVCVFNELICLGHSVLVIEHNLDVLNTADWIIELGPAG
                     GRQGGLVVFEGTPDQIVKEVSSFTGRALRDWRNVLDGQEVSRESFFNFPPMKLSAPED
                     NTRIVIQGAREHNLKNLSVTIPRDKFTVVTGPSGSGKSTLAFDLVFAEGQRRYLESLN
                     AYARSMVQLAPAPEVDSIRGIPPTVAIEQRTSRGGLRSTVGTTTELLHFLRLLFVKLG
                     TQYCPDCGIPASPQSHDDIIKAIRRDFGSDQIAVLAPIVRRQKGIFRAEMEAAAVAGH
                     RIMRIDGAYIDISLKIPSLARYSEHELELAVGICGADEQALEKIVAAALLHGHGRLLV
                     TRSLPDEPTTGLLEQDVFYSTLAACPSCLKSFPALDPKLFSYNSSAGACPKCSGYGLI
                     TSALKKAQKKKEDFNTDLSDTEGAELCPDCLGTRLNPVARAVRWHDRSISDFGAMTID
                     SVEEFFRNLRLEGREAAIAHDAVAEILSRLSFLQEVGLGYLTLDRSAPTLSGGEAQRI
                     RLAAQLGSNLRGVCYVLDEPTIGLHPRDNRILLDAIDRLTHQGNTLLVVEHDEDTIRQ
                     ADHIIDIGPGSGVRGGELVAEGTVDDIMKCERSLTGEYLRNPSKHRHFASRPFNPETD
                     PKLSVINPRMHNLAGGVVEFPLNRLTVVTGVSGSGKSTLTREVLFENLIGNLGSDPSK
                     AKWKGCDIITGREPVARVLEVDQTPIGKTPRSCPATYVGFFDKIRALFAGTNEGKARG
                     YGVGRFSFNNEGGRCEACAGQGLRTIEMSFLPDVKVPCEVCHGARFNQETLAVTWKDR
                     NIGEILQMQVDEAVEFFNSMPTIAHPLKLLQDIGLGYLTLGQPSPTLSGGEAQRIKLV
                     SELTKVKDSVSTRGRKSPHTLYVLDEPTVGLHMADVDKLIGVLKRLVDAGNTVIVVEH
                     NLDLMAQADWIVDMGPEGGANGGRVVAAGTPETLTSTNTHTGRALKAHMDAH"
BASE COUNT          984 a         1644 c         1424 g          877 t
ORIGIN      
        1 caagtccttt tcccagccct ccgcttctct cttcagcttc aattctcccg taggcgcctg
       61 cgagaaatgc aggggcttcg gccgcatcat tacggtggat ccgtcgctcg tgatccccga
      121 tccctcgaaa accctcgagg agggagccgt caagccatgg acgacaaagt ccttcaacga
      181 atgccagcag gagatgcttg cctacgccgc cgtgcgcgac attcgcacca acacgcccta
      241 caaaaagctc acggctgagg agaagcgctg ggtctgggag ggcggcgagg actggacggg
      301 caactggcgc acgcagtggt acggcatcaa tcgctttttc gaatggctcg agagcaaggc
      361 ctacaagatg cacgtccgca tgctgctgtc acgctaccgt tcctacacgg agtgcccctc
      421 gtgccgcggc tcccacctga agcagccgtc gctcttctgg cgatacggca ccctcgagga
      481 aaagcagctc gtgcttgcaa agaccatcga aggcggcgtc ttcaagccga tcggcatgac
      541 cctttccgac cgtgcctacg cgaagctgcc cggcttcaac taccacgagc tcatgacgct
      601 cccgatcgaa gacctgcggg acttcttcaa aaaacgccgc gcacgcgccg acgaatccga
      661 gcagatgctg ctggacgaaa tctgctcgcg cctgacgttc ctttgcgacg tgggcgtcgg
      721 ctacctgacg ctcgaccgac agagccgcac gctctcgggc ggtgaagtgc agcgcgtgag
      781 cctcacgacg gcactgggca cggccctcgt caacacgctg tttgttctgg acgaaccttc
      841 catcggcctg catccgcgcg acatgcatcg cgtcaaccgc atcatgcgcc gcctgaccga
      901 agcgggcaac acgcttgtcg tcgtggagca tgaccctcag gtcatgctcg ctgcaaaccg
      961 cctcatcgac atgggcccgg ggtcgggcac ggacggcggc cggatcatct tcgacggcag
     1021 cacgcgtgaa gtgctcgacg ctggcaccct gacaggcgac tacctgtcgg gccgcaggcg
     1081 cgtgcggcgt cccgatccag aattcgtgac ggccgacagg ccccgcctga tcatcaaggg
     1141 tgcatcagaa cacaatctcc gccacatcga cgtggccatt ccgatggggg ccatcgtcgc
     1201 catcgcaggc gtctcgggtt cgggcaaatc gacgctcgtc tccgacatcc tcgtgcccgc
     1261 ccttaagcgc aagtccggca aaagcaccga ccagcccggc aagttcgacg gactggcggg
     1321 atgcattccc catgatgtga tctacgtcga ccagagtccc atcgggcgca caacccgcgg
     1381 gaaccccgtg agctacgtcg gcagcttcga cggcatccga tcgctttttg cggccacgcc
     1441 catggcccgc catctggggc tcagcgtcgg cgacttctcg ttcaactccg gcaagggacg
     1501 ctgtccgacc tgcggcggcg ccggcttcga acacatcgag atgcagttcc tctcggacgt
     1561 ctatctgccc tgccccgcct gcggcggcaa gcgctacaag gactctactc tggaggtgaa
     1621 gatccgtctc tcggacgacc gcgactacag catcgccgac gtgctagaca tgacggcaga
     1681 tgaagccacg cgacttttct ttgaataccc cagcatcacc tggggaatcc aacagctttg
     1741 caaagtgggc ctcggctatc tgaagctcgg gcagccgctg accacgcttt ccggcggcga
     1801 acgccagcgc ctcaagctcg caggcaccct ctccgagggg ctttcgctcg gacgtcgctg
     1861 cggctacagc gctgggaagc tcttcgtctt cgacgaaccg acgacaggac tgcactttgg
     1921 cgacacggcg acgctcgttt gcgtatttaa cgaactgatc tgcctcggac attcggtgtt
     1981 ggtgatcgaa cacaaccttg acgtcctcaa tacggccgac tggatcattg aactcggtcc
     2041 cgccggcgga cgtcagggcg gcctcgtggt cttcgaaggc acgcccgacc agatcgtcaa
     2101 ggaggtcagc tccttcacgg gacgcgcact cagggactgg cgaaacgtcc ttgacgggca
     2161 ggaagtctcc cgggaaagct tcttcaactt cccgccgatg aagctcagcg cccccgagga
     2221 caacacccgg atcgtcattc agggcgccag ggagcacaac ctcaagaatc tctcggtcac
     2281 gattccccgg gacaagttca cggttgtcac gggcccctcg ggctccggca agtcgacgct
     2341 tgccttcgac ctcgtcttcg cggaaggaca gaggcgttat cttgaaagcc tcaatgccta
     2401 tgcgcgaagc atggtccagc tcgctcccgc ccccgaggtc gactcaatcc gcggcattcc
     2461 gcccacggtt gccattgagc agcgtacctc gcgcggtggc ctgcgctcaa ccgtcggcac
     2521 gacgacggag ctcctgcatt tcctccgcct gcttttcgtc aagctaggca cccagtactg
     2581 cccggactgc ggcatcccgg caagccccca gtcccacgac gacatcatca aggcaatccg
     2641 ccgcgacttc ggctcggacc aaatcgccgt gcttgcgccg atcgtgcgtc gccagaaggg
     2701 catcttcaga gcggaaatgg aggcggccgc agtcgccgga cacaggatca tgcgcatcga
     2761 tggagcatat atcgacatat ccctcaaaat cccttcgctc gcccgttatt cggaacacga
     2821 gctcgagctt gccgtcggga tatgcggtgc cgacgagcaa gcgctggaaa agatcgtggc
     2881 cgccgcactc cttcacgggc acggtcgcct gcttgtgacg cgttcgcttc ccgacgaacc
     2941 gacgacaggc ctgctcgaac aggacgtctt ctactcgacg ctcgcagcct gcccctcctg
     3001 cctcaagagc ttccctgctc tggatccgaa gcttttctcc tacaactcga gcgcaggcgc
     3061 gtgtccgaag tgctcaggct acggtctcat cacttcggcg ctcaagaagg cgcagaaaaa
     3121 gaaggaggac ttcaacaccg acctcagcga cacggagggg gcggagcttt gccccgactg
     3181 tctggggact cgtctgaatc ccgttgcaag ggcggtgcgc tggcacgaca gaagcatttc
     3241 ggacttcggt gcaatgacga tcgacagcgt ggaggagttc ttccgaaacc tcaggctcga
     3301 gggacgcgag gcggccattg ctcacgacgc ggtcgccgag atcctgtctc gcctctcctt
     3361 ccttcaggaa gtgggactcg gatacctcac gctcgaccgc agcgcgccga cgctttccgg
     3421 cggtgaagcc cagcgcattc gtctggccgc acagctcggc agcaacctca ggggtgtctg
     3481 ctacgtgctc gacgagccga cgataggact gcatcctcga gacaaccgca tccttcttga
     3541 cgccatcgac cggctcaccc accagggcaa cacgctgctc gtcgtcgagc atgacgagga
     3601 cacgatccgt caggcggacc atatcatcga catcggtccc ggctccggcg ttcgaggcgg
     3661 cgaactcgtt gccgagggca cggttgacga catcatgaag tgcgagcgct cacttacggg
     3721 tgaatacctg agaaatccga gcaagcacag gcattttgcc agccgtccct tcaatcccga
     3781 gacagacccg aagctgtcgg tgatcaatcc ccgcatgcac aatctggcag gcggagtcgt
     3841 ggaattcccg ctcaaccgcc tgaccgtcgt gacgggcgtc tccggctccg gcaagtccac
     3901 tctgacccgt gaagtgcttt ttgaaaatct gatcggcaat ctcggctccg atccgagcaa
     3961 ggcgaagtgg aagggatgcg acataatcac gggccgcgaa ccggtggcgc gtgttctgga
     4021 agtggatcaa acccccatcg gcaagacgcc gagatcgtgt cccgccacct acgtcggctt
     4081 ctttgacaaa atccgcgcgc tctttgcagg cacgaatgaa ggcaaggctc gaggttacgg
     4141 cgtgggacgc ttctccttca acaacgaggg cggccgctgc gaagcctgcg ccggacaggg
     4201 actgcgcaca atcgagatgt cgttcctgcc tgacgtcaag gtcccctgcg aggtgtgcca
     4261 cggcgcacgc ttcaatcagg aaacgctggc cgtcacctgg aaggatcgaa acatcggaga
     4321 aatcctgcag atgcaggtcg atgaagcagt cgagtttttc aactcgatgc cgacgatcgc
     4381 acacccgctc aagctccttc aggacatcgg tctgggctac ctcacgcttg gccagccctc
     4441 acccacgctt tccggcggcg aggcgcagcg catcaagctc gtgagcgagc tcaccaaggt
     4501 caaggattcc gtctccacca ggggacgcaa gagtccgcac acgctctacg tgcttgacga
     4561 accgacggtg ggcctgcaca tggccgacgt cgacaagctc atcggcgtgc tcaagcgcct
     4621 cgtcgacgca ggcaacacgg tgattgtcgt cgaacacaat ctcgacctga tggcgcaggc
     4681 cgactggatc gtcgacatgg gccccgaggg cggagccaac ggcggcagag tcgtggccgc
     4741 gggcactccc gagacgctca cctccacgaa cacccatacg ggccgcgcgc tcaaggccca
     4801 catggatgcc cactaggaca cggtatgaaa tcggataggg gtgaggactt ttgatcctca
     4861 gccccttcca caccacccac cgtgcggatc cgcagtgggc ggttatcaat aacctcagag
     4921 aacttccta
//
LOCUS       DBMDTI010000141         4920 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4131_length_4920_cov_1.803071, whole genome shotgun sequence.
ACCESSION   DBMDTI010000141 DBMDTI010000000
VERSION     DBMDTI010000141.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4131_length_4920_cov_1.803071"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="ACZPB6_07105"
     CDS             <1..400
                     /locus_tag="ACZPB6_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HIR1184269.1"
                     /translation="PTSVARLFSYTRVVGETFKANGNTLGIDDYDSMVGRIGATAGLN
                     CSQGRGGVYARFGVAHEFMGDGRFTAVNAAGTAADPIEVDGKDTWVEYAVGANFNITK
                     QTYVWADLERTSGAEVDEDWRATIGVRHAF"
     gene            complement(535..1374)
                     /locus_tag="ACZPB6_07110"
     CDS             complement(535..1374)
                     /locus_tag="ACZPB6_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013122.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR1184270.1"
                     /translation="MAQLHHQVNHRYDRHPSARRPSERLCRNASAFRKASRRPSRPAR
                     LALARTLEASCPPSRTGRRRGPSQEPFHAGTHFNPDLLTSEAERRARLEGVHAALDRA
                     ESLVFLDTESTGGRNGRLIEVALVETDVEQNITGGLHFRCNPHRRSQAGARRVHGIQD
                     CELEHCPEFAARADELLEAVRGKTVVIHDRTMDLLWLNRELQAARPGAPRFEDCCTVV
                     DSFVLARAIPSERRRNGLDALLEWYGLGARGGHHDAYGDAALLSRVFFELWWDLDEWL
                     YGE"
     gene            1544..1996
                     /locus_tag="ACZPB6_07115"
     CDS             1544..1996
                     /locus_tag="ACZPB6_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184271.1"
                     /translation="MAYTAYKFKPSFVEKRLKNYKSNLMKFKGHDNLVANAVRVIRER
                     LEADPRRYLSYGPYWWGIKKVLVDNGVPLGECDDEEIRLAYSGATDEETIVMAEEFRS
                     DCLDNMMLYTDRFMLDEESGRWWRLRDPDMAAAGAMAAPDIIDYMREF"
     gene            2284..3591
                     /locus_tag="ACZPB6_07120"
     CDS             2284..3591
                     /locus_tag="ACZPB6_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HIR1184272.1"
                     /translation="MEFILNPIVISVVVLCALCLARVNVLLALIAASLVAGLTGGLGL
                     TKSMELLSGGFSANATTALSYILLGTFAVAIAHTGLMQMLVRWIGAKMGGMPLVFCFG
                     LAFIACLSQNVVPVHIAFIPLLIPPLLKLMNKMKLDRRAVSCALGFGLTAPYVSLPIG
                     FGLIFQGIVAQSMTQNGMAVTVAYVASEAWIAGLSMVVGLVLAVFYFTRRPRTYLDKP
                     VVGVTESDEEIRFGLRHWVTLGAIVAAVAVQIMLESLPLAALLGLIIMLAGGAFQFKK
                     LDDHIASGISMMGFIAFVMLIAGGYAAILKETGAVNSLIEGVVPLMGESKWVAATIIT
                     LIGLLVTMGIGTSFGTVPILAVIYVPLCTAVGFSVPATILLMTIAGALGDAGSPASDS
                     TLGPTSGLNADGQHNHIWDTCVPTFLAYNVTLLVAGIVVSQFI"
     gene            complement(3682..4059)
                     /locus_tag="ACZPB6_07125"
     CDS             complement(3682..4059)
                     /locus_tag="ACZPB6_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HIR1184273.1"
                     /translation="MKKTLAALAGALVALPVLAAGPQGFAAAPQAQGPKGFDTEPATT
                     VAAVLESGKDHQIVTLRGKFTEHVRGDKYVFTDDKGASIRAELDHDRDWSMITKDVPV
                     EIRAEVDKDWTEIELDVLSAKPL"
     gene            4277..4882
                     /locus_tag="ACZPB6_07130"
     CDS             4277..4882
                     /locus_tag="ACZPB6_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012774.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR1184274.1"
                     /translation="MTGAPAPAVLVMGGFIVLLLLWLVHHLSRRRRFPLVFEAKVEHV
                     CDGDSIIVRGRIGRVKLRIAGMDAPETAQARGRESERRLRAMLQGRVVKVRSVAADRY
                     GRWVSTVTADGVDAGLVMVEEGEAWAYRTYLWALPKEARGRYLAAEARARRAGRGLWA
                     ERSPEAPWLWRRRHRTILQRILDFFWRIGRAVRRFFWGVGK"
BASE COUNT          823 a         1549 c         1630 g          918 t
ORIGIN      
        1 tcccacttca gtggcgaggt tgtttagcta tacgcgcgtt gtcggcgaaa cgttcaaggc
       61 caacggcaac acgctcggca tcgacgacta cgactccatg gtcggccgca tcggcgcgac
      121 cgcaggcctg aactgctcgc agggccgcgg cggcgtgtac gcccgcttcg gcgttgcgca
      181 cgagttcatg ggcgacggca ggttcacggc cgtgaacgcc gccggcaccg ccgccgatcc
      241 gatcgaagtc gacggcaagg acacctgggt cgagtacgcc gtgggtgcga acttcaacat
      301 cacgaagcag acctacgtct gggccgacct tgagcgcacg tccggcgccg aagtcgacga
      361 ggactggcgc gccaccatcg gcgttcgcca cgccttctga tgtccgactg acgccgcagg
      421 catgacgtcc gtcatgccgc ggcgaaggtt gccatgacaa aaaggggccc gccctctcat
      481 cagccgatga gaaggcgggc tcttcctttt tgcgtcttga atgccttccc gtcctcattc
      541 tccgtagagc cattcgtcga ggtcccacca gagttcgaag aagacgcggg agaggagggc
      601 cgcgtcgccg taggcgtcgt ggtgtccgcc tcgagcgccg agtccgtacc attcgagcag
      661 ggcgtcgagc ccgttgcggc ggcgctccga cggaatcgcc ctggcgagca cgaacgagtc
      721 gacgacggtg cagcagtcct cgaacctcgg cgcgccggga cgcgcggcct gaagctcccg
      781 attgagccag agaaggtcca tcgtgcggtc atggatcacg acggtcttgc cgcgcacggc
      841 ctcgagcagc tcgtccgcac gggccgcaaa ctcgggacag tgctcgagct cgcagtcctg
      901 aatgccgtgc acgcgccttg cgcccgcctg ggagcgtcgg tgcggattgc agcgaaagtg
      961 caggccgccc gtgatgttct gctcgacgtc cgtctcgacg agggccacct cgatgaggcg
     1021 cccgttgcgc ccgcccgtcg attcggtgtc gagaaagacg agggactcgg ccctgtcgag
     1081 cgccgcatgt acgccttcga gcctcgcgcg gcgctccgcc tccgacgtga gaaggtcggg
     1141 attgaaatgc gtgcctgcat gaaaaggctc ttgcgaaggg cctcgccgtc gtcctgtacg
     1201 ggaaggcggg caagaagctt cgagagtgcg ggcgagagcg aggcgggcgg gacgggacgg
     1261 gcggcgggat gcctttcgga aggcagaagc gttgcggcag aggcgttcgg aaggacggcg
     1321 tgcggaagga tgacggtcat aacggtgatt tacttgatga tgcagttggg ccatgttagg
     1381 cggctgtgcg tcagttcgtg acgcaggagc gcctctcctg aacgccgttc cggctgcgtc
     1441 aggagttgac gcatgggggc cgatactgaa gaaaacggag ggggatccgc aggggccgcc
     1501 atgtcagggc ccgcgttctc ctcccgaatc tggagtcaaa gacatggcat acaccgccta
     1561 caagttcaag ccgtcatttg ttgagaagcg tctcaagaac tacaagtcga acctgatgaa
     1621 gttcaaggga cacgacaatc tcgtcgccaa cgccgttcgc gtgatcaggg agaggctcga
     1681 ggccgatccg cgccgctatc tttcctacgg gccctactgg tggggcatca agaaggtgct
     1741 cgtcgacaac ggcgtgccgc tcggcgaatg cgacgacgag gagattaggc tcgcctactc
     1801 gggcgcgacc gacgaggaga ccatcgtgat ggccgaggag ttccggagcg actgtctcga
     1861 caacatgatg ctctacaccg accgcttcat gctcgacgag gagtcgggca ggtggtggag
     1921 gcttcgcgat cccgacatgg cagcggcggg cgccatggct gcgccggaca tcatcgatta
     1981 catgcgcgag ttctgacgca ggccccgccg cgggggcttc ggcagatgcg ggcgccttgc
     2041 acgacgggcg cccgtggtcg ttccgcgcac cggactgtcg agtggcgcct ctcctgctga
     2101 gggtgttggg tgcattctca gtcttttggg ttaggaaaag acatccttcg ggatgtgtgg
     2161 aatcgttgca gactgtgtca taatcgaacg cgtatgtccc gcaaaagggc gccaccgtcc
     2221 cccgagggcg tccgccggca gcgggatcct gacagaagac agaatagaaa aggaagtcca
     2281 gccgtggaat tcatcctcaa tccgatcgtc atatcggtcg tcgtgctctg cgcgctctgc
     2341 cttgcgcgcg tcaatgttct tctcgccctg attgcggcct ctctggtcgc gggtctcacg
     2401 ggcgggctcg gcctcacgaa gtcgatggag ctgctctcgg gcggcttcag cgccaacgcg
     2461 acgaccgcgc tctcctacat ccttctgggc actttcgccg tggcgatcgc gcacacgggc
     2521 ctcatgcaga tgctcgtgcg ctggatcggc gccaagatgg gcggcatgcc gcttgtcttc
     2581 tgcttcggcc tggcgttcat cgcctgcctt tcgcagaacg tggtgcccgt gcacatcgcc
     2641 ttcattccgc ttctgattcc gccgctgctc aagctcatga acaagatgaa gcttgaccgc
     2701 cgcgcggtct cctgtgctct cggcttcggc cttacggctc cgtacgtgtc gctgcccatc
     2761 ggcttcggcc tcatcttcca gggcatcgtc gcgcagtcca tgacgcagaa cggcatggcc
     2821 gtgacggtgg cgtacgtggc gtcggaggcc tggatcgccg gcctctcgat ggtcgtcggt
     2881 ctcgtgctcg ccgtcttcta cttcacccgc cgtccccgca cctatctcga caagcccgtc
     2941 gtgggcgtga cggagtccga cgaggagatc cgcttcggtc tcaggcactg ggtcacgctc
     3001 ggtgcgatcg tcgccgccgt cgcagtgcag atcatgctcg agagcctgcc gctcgctgcg
     3061 ctgctcggcc tcatcatcat gctcgcgggc ggcgccttcc agttcaagaa gcttgacgac
     3121 cacatcgcga gcggcatttc catgatgggc ttcatcgcct tcgtgatgct gattgcgggc
     3181 ggttatgccg cgatcctcaa ggaaacgggc gctgtcaact cgctcatcga gggcgtcgtg
     3241 cccctcatgg gcgaaagcaa gtgggtcgcc gcgacgatca tcacgctcat cggcctgctc
     3301 gtcacgatgg gcatcggcac gtcgttcggc acggtgccca tcctcgccgt catctacgtt
     3361 ccgctctgca cggccgtggg cttctccgtc cccgccacga tcctcctgat gacgatcgcg
     3421 ggcgccctgg gcgacgcggg ctcccccgcc tccgactcca cgctcggccc cacgtccggc
     3481 ctcaacgccg acggccagca caaccacatc tgggatacct gcgtgccgac cttcctcgcg
     3541 tacaacgtga cgcttctcgt ggccggcatc gtcgtctcgc agttcatctg acgaagattc
     3601 acgccggatt tcgtcgcaac tgacaaaacg cccgcaggcc ggaaggcccg cgggcggtgt
     3661 cgttcatggc ggcggaaagc ctcagagcgg ctttgcggag aggacgtcga gttcgatttc
     3721 ggtccagtcc ttgtccacct cggcgcggat ttcgacgggt acgtccttcg tgatcatgga
     3781 ccagtcgcgg tcatgatcga gctcggccct gatggaggcg cccttgtcgt cggtgaagac
     3841 gtacttgtcg ccgcggacgt gttcggtgaa ttttccgcgc agcgtgacga tctggtggtc
     3901 cttcccggac tcgagcacgg cggcgacggt ggtggcgggc tcggtgtcga agcccttcgg
     3961 accctgggcc tgaggcgccg cggcaaagcc ctgcgggccg gcggcaagga cgggcagggc
     4021 gacaagtgcg ccggcaaggg cggcgagcgt cttcttcatg tgttctctcc tgagtggcca
     4081 taatacggct cgcgaaaaaa gaaaatgcgg ttttttgaga gcgccttcgc ggcagggcga
     4141 aggccgtgga aacattctat tgagaaggct tctcgtttcg catccctcat ttcgcaagga
     4201 aaacgcagaa cgtgtaacga aagacaacca tgcgcgcatc gggaaaacaa actgaaagat
     4261 tcttcggagg ccggccgtga cgggcgcgcc cgcacccgcc gtcctcgtga tgggcggctt
     4321 catcgtgctt cttctgctct ggcttgtcca tcatctctcg aggcgcaggc gcttcccact
     4381 cgtcttcgag gcgaaggtcg aacacgtctg cgacggggac tcgatcatcg tccgcggccg
     4441 catcggccgt gtgaagctca ggattgcggg catggatgcg cccgagacgg cacaagcgcg
     4501 cggccgcgag tcggagaggc ggcttcgcgc catgctgcag ggaagagtcg tgaaggtgag
     4561 gagcgttgcg gccgaccgat acggccgatg ggtgagcacc gtgaccgcgg acggcgtcga
     4621 tgcgggactc gtcatggtcg aggaaggcga ggcctgggcc taccgcacgt atctgtgggc
     4681 gctcccgaag gaggcccgcg ggcgttatct cgcggccgag gcgcgcgcaa ggcgcgcggg
     4741 acgcgggctc tgggcggagc gcagtcccga ggcgccctgg ctctggcgca ggcgtcaccg
     4801 caccatcctg cagcgcattc tcgacttctt ctggcggatc ggacgcgccg tcaggcgctt
     4861 cttctggggc gtcggaaaat aggcggctct tgcaaacatt ttcgcatgca gggcgtgcgt
//
LOCUS       DBMDTI010000142         4919 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4133_length_4919_cov_3.105645, whole genome shotgun sequence.
ACCESSION   DBMDTI010000142 DBMDTI010000000
VERSION     DBMDTI010000142.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4133_length_4919_cov_3.105645"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            144..1094
                     /locus_tag="ACZPB6_07135"
     CDS             144..1094
                     /locus_tag="ACZPB6_07135"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR1184275.1"
                     /translation="MLSAADSLMWDALEIGPQWILRETEDVTLAETALRAELRQPAPA
                     VRSAAPAAPSRPAPRPAARSTALPPGMPPEFAPRPAPARPAPVVRPVAEPISSAPQAP
                     ALVLPPEVVAACEKADWATLEGLAGACTCCSMAKSRQHVVFAEGGPGMRIALVGEAPG
                     SEEDLQGIPFVGKSGQLLTQMLESIGIERRRDIAIMNVLKCRPPGNRNPAPAEVACCA
                     TYLRRQLLLVRPDVLLIMGRFAMQTLLGLGPDASIGSQRGRVHDVTIGDFDTKAVVSY
                     HPSYLLRAPDEKAKAWEDLTLFRRLIREAGIKLAPVHHTH"
     gene            1185..1526
                     /locus_tag="ACZPB6_07140"
     CDS             1185..1526
                     /locus_tag="ACZPB6_07140"
                     /inference="COORDINATES: protein motif:HMM:NF017958.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HIR1184276.1"
                     /translation="MTNNNEVRDAVSPDRTLYDRALRASGREAAATLAAAAFTMAYFW
                     AAVWLLEDSDMTILSMPVWFMASCVGGYFVSVAAVWVLVKRCFTDIDLDEAARGYSAG
                     NLEKAGGEDQS"
     gene            1523..3031
                     /gene="panF"
                     /locus_tag="ACZPB6_07145"
     CDS             1523..3031
                     /gene="panF"
                     /locus_tag="ACZPB6_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HIR1184277.1"
                     /translation="MTNTFLILLPIALFLVMLMGLSLWAERAGRRNRFAGEYFLGDRS
                     LKGFVLAMTLVATYGSVSSFVSGPGLAWQLGLGWVVFAAPQIIAGFLILGVAGKKMAV
                     VSRAAGALTVIDMIRARYGDGRGGRTLAALLAVLMLVFFTTMMIGQFIGGAQIFSKAA
                     GVSYGWGLVLFGAVTVLYTAFGGFRAVAVTDTACAVLMLVGMGALGWNILETGGGLAQ
                     VMANVAAAAPRGAGSEGVLLSPTSGGALATSLLLSAWVLVGFGTLGLPQSAVRCMSYR
                     RSSDLHQAMIVSTVVCGALMIGMTVMGVFARGMLDVPLSEMGGTTDAVMPYLIANYMD
                     PVTAGVTLIGPLAATMSTVSSLLLAAASAIMKDLVLFHRPEAGRNEARLRMTTRLMTG
                     ALGLLALILALEPLDVIAWINLFAFGGLELAFLMPLIGGLFWRGATAGGALVSVAGSI
                     AFYLAVTIFKIPMGGAHAIAPCMVVAAVLFVAGSRLTPASPAKAIDLFFPRR"
     gene            complement(3138..3710)
                     /locus_tag="ACZPB6_07150"
     CDS             complement(3138..3710)
                     /locus_tag="ACZPB6_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR1184278.1"
                     /translation="MMKPIDLDRAYRLLNIGATTLVSAAHEGDADIMAAAWATALDLT
                     PCKATVVIDKSHYTRKLIEKSGFFALMIPTAAIAPIVMNLGTVSKNDNPRKLEECGTR
                     LVTLPEYDMPLLEGCAAWIVFRVVPELHNQEAYDLFIGEAVAAFADDRVFVNGRWNFE
                     EAPDDLRTLHYVAGGHFYTIGDALEVPASA"
     gene            complement(3812..4453)
                     /locus_tag="ACZPB6_07155"
     CDS             complement(3812..4453)
                     /locus_tag="ACZPB6_07155"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR1184279.1"
                     /translation="MQNFHELIQGFHNFKEDYFLREREFFSSLVHGQSPKTLVVACCD
                     SRVDPAILMGCRPGDLFVARSIAAIVPDVEKAGEHDAVVSAVEYAVKHLDVRNIIVMG
                     HSNCGGIHGLLHPEVVSKEPYISRWLCLAHPVLEELEHEDPDEPADVRARRCEEGTVL
                     QSIDSLLSYDWVKSRVDAGALALHALYYDLASGTMYVWNAEAEDFEATKLSEL"
     gene            4793..>4919
                     /locus_tag="ACZPB6_07160"
     CDS             4793..>4919
                     /locus_tag="ACZPB6_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012707.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1184280.1"
                     /translation="MVVGTQAGAETLRVGSETVYPPFEFLDSNSGKYVGFDIDLID"
BASE COUNT          836 a         1535 c         1577 g          971 t
ORIGIN      
        1 gaggtgcgcg aatcgaacga gcgtgtgatc cgtctttacg aaacctcggg tttcgtgcgc
       61 gtgggtcttc gcaagaacta ctaccgcacg gccgaaggcc gcgagcatgc ggtcctgatg
      121 acgcttgaat tcggaggtga ggcatgctga gcgccgccga cagcctgatg tgggacgccc
      181 tcgagatcgg tccgcagtgg attctgcgcg agaccgaaga cgtgacgctt gccgaaaccg
      241 cgcttcgcgc cgagctcagg cagcccgcac cggccgttcg ctccgccgcg cccgctgctc
      301 cttcgaggcc tgcgccgcgt cccgccgcac gatccaccgc actgccgccg ggaatgcctc
      361 ccgagttcgc gccccgtccc gcacccgcga gacccgcccc cgtcgttcgt cccgtcgccg
      421 aaccgatttc aagcgccccg caggcgcccg cactcgtgct tccgcccgaa gttgtcgccg
      481 cctgtgaaaa ggctgactgg gcgacgcttg aagggcttgc aggcgcatgc acctgctgct
      541 cgatggccaa aagccgccag cacgtcgtct tcgccgaagg cggtcccggg atgcgcattg
      601 cgctcgtggg cgaggcgccc ggcagcgagg aggaccttca gggcattccg ttcgtcggca
      661 agtcgggcca gctgctcacg cagatgctcg agagtatcgg catcgagcgc cgccgcgaca
      721 tcgcgatcat gaacgtgctc aagtgccgcc cgccgggaaa ccgcaatccc gcgcccgccg
      781 aggtggcctg ctgcgcaacc tatctgcgca ggcagcttct tctcgttcgc cccgacgtgc
      841 ttctcatcat gggacgcttc gccatgcaga cgctgctcgg tctgggaccc gacgcttcga
      901 tcggcagcca gcgaggacgt gtgcacgacg tgacgatcgg cgacttcgac acgaaggccg
      961 tcgtcagcta tcatccgtcc tacctgcttc gagctcccga tgaaaaggcc aaggcctggg
     1021 aggatctgac gctcttcagg cgtctgatcc gcgaagcggg catcaaactc gctcccgtcc
     1081 atcacacaca ctgacaacaa ggacggggta tttccccaat ggcgcaaaga cgccgaagaa
     1141 gccgtcgtca tcctcatggg cgagggccgg aggtaacatc gtcaatgacg aacaacaacg
     1201 aggtccggga cgccgtgtcg ccggaccgca ctctctatga ccgcgcactt cgcgcttcgg
     1261 ggcgcgaggc ggcggccacg ctcgccgccg cggccttcac gatggcgtac ttctgggcgg
     1321 cggtgtggct ccttgaggat tcggatatga cgatcctctc catgcccgtc tggttcatgg
     1381 catcctgcgt cggcggctat ttcgtcagcg tcgcggccgt ctgggtgctc gtgaagcgct
     1441 gtttcaccga cattgacctt gatgaagccg cccgcggcta ctcggcgggg aaccttgaga
     1501 aggccggcgg ggaggatcag tcgtgacgaa caccttcctg attctccttc cgatcgcgct
     1561 ctttctcgtc atgctcatgg ggctttcgct ctgggccgag cgcgcgggtc gccgcaaccg
     1621 ctttgcaggc gaatacttcc tcggcgaccg cagcctcaag ggcttcgtgc tcgccatgac
     1681 gctcgttgcg acctacggat ccgtctcctc cttcgtttcg gggcccggcc ttgcctggca
     1741 actcggcctc ggctgggtgg tcttcgccgc gccgcagatc atcgcgggct ttctgattct
     1801 cggcgtggcc ggcaagaaga tggccgtcgt ctcgcgcgcc gcgggggcgc tgaccgtcat
     1861 cgacatgatc cgcgcacgtt acggcgacgg ccggggcgga cggacgcttg cggcgctgct
     1921 cgcagtgctg atgctcgtct tcttcacgac gatgatgatc ggtcagttca tcggcggcgc
     1981 acagatcttc tcgaaggccg cgggcgtgag ctacggctgg gggctcgtgc tcttcggcgc
     2041 cgtcaccgtg ctctacacgg ccttcggcgg cttcagggcc gtggccgtta ccgacacggc
     2101 ctgcgccgtt ctgatgctcg tgggcatggg cgcgctcggc tggaacatcc tcgagacggg
     2161 cggcggactc gcccaggtga tggcgaacgt cgccgcagca gctccccgcg gggcgggcag
     2221 tgagggcgtg ctgctctcgc ccacctcggg cggcgcgcta gcaacgtcgc tgcttttgtc
     2281 cgcatgggtg ctcgtgggct tcggcacgct cgggttgccc cagtcggccg tgcgctgcat
     2341 gagctaccgc cgctcctccg acctgcatca ggccatgatc gtctcgaccg tcgtctgtgg
     2401 tgcgctcatg atcggcatga ccgtgatggg cgtctttgcc cgaggcatgc tcgacgtgcc
     2461 tctctccgag atgggcggca cgacggacgc cgtgatgcct tacctgatcg ccaactacat
     2521 ggatcccgta acggcgggcg tgacgctcat cggccctctg gccgccacga tgtcgacggt
     2581 gagttcgctt cttctcgcgg ccgcctccgc catcatgaag gacctcgtgc tctttcaccg
     2641 cccagaggcg ggccgcaacg aggccaggct tcgcatgacg acgaggctca tgacgggggc
     2701 gctcggactc cttgcgctga ttctggcgct cgagcctctt gacgtcatcg cctggatcaa
     2761 tctctttgcg ttcggcggcc ttgaactcgc cttcctgatg ccgctgatcg gcggtctttt
     2821 ctggcgcggc gcaacggcag gaggtgcact tgtttccgtt gcgggcagca tcgccttcta
     2881 tctcgccgtc acgatcttca agattccgat gggaggcgcg catgccatcg caccctgcat
     2941 ggtcgtggcg gccgtgctct tcgttgctgg ctcaaggctg acgcccgcat ctcccgcgaa
     3001 ggcgatcgac ctcttcttcc cgaggcgatg acgggcggaa gggctttgcc ccaataggga
     3061 gaaatgaaaa aggagttggg cgctggctca actccttttt tgatttccgg gctttcagca
     3121 aaaagccgca tcttgactca ggcggaggcc ggaacttcaa gcgcgtcgcc aatggtgtag
     3181 aaatggccgc cggccacgta gtgcagcgtt cgcaagtcgt cgggagcctc ttcgaagttc
     3241 caacggccgt tgacgaagac gcgatcgtcg gcaaaggcgg cgacggcttc accaatgaac
     3301 agatcgtagg cttcctggtt gtgcaactcg ggcacgacgc ggaagacgat ccaggcggca
     3361 cagccttcga gcaagggcat gtcatattcg ggaagcgtca cgagacgagt accgcattcc
     3421 tcaagcttgc gcggattgtc gttcttgctg accgtaccga gattcatgac gatcggagcg
     3481 atggctgccg ttggaatcat cagagcgaaa aaaccgcttt tttcgatgag cttgcgggtg
     3541 tagtggctct tgtcgatgac gactgtggcc ttgcacggcg tcaggtcgag agctgtagcc
     3601 caggcggcgg ccatgatgtc tgcgtcgccc tcgtgagccg cgctcacgag cgtcgtggcg
     3661 ccgatgttca aaagtcgata tgcgcggtct agatcgattg gcttcatcat gatttgaaat
     3721 gtttcccgtt gttggagtct ggaagctagg agttcagtcg gagagaattc gcggtgcgag
     3781 ggccgggcgc ggaaaagtct gcgcagcttg ttcagagctc ggagagcttg gttgcctcga
     3841 agtcctccgc ttcggcattc caaacgtaca tggtaccgct ggcaaggtcg tagtagaggg
     3901 cgtggagtgc gagcgcgcct gcatcgacgc gggacttgac ccagtcgtag gagagcaggc
     3961 tgtcgatgga ctgcagtacg gtgccttcct cacagcgtcg tgcgcggacg tcggcgggtt
     4021 cgtcgggatc ctcatgctcg agttcttcga ggacgggatg ggcgaggcag agccagcgag
     4081 agatgtaggg ctccttcgag acgacttcag ggtgcaggag gccgtgaatg ccgccgcagt
     4141 tcgagtgtcc catgacgatg atgttgcgca cgtcgaggtg cttcacggcg tattcgacgg
     4201 cggagacgac cgcatcatgt tcgcctgcct tctcgacatc gggaacgatc gcggcaatgc
     4261 ttcgggcgac gaagaggtcg cccggacggc accccatcag aatggcgggg tcgacgcgcg
     4321 agtcgcagca ggctacgacg agcgtcttcg gactctggcc gtggacgagg ctgctgaaga
     4381 attcgcgctc gcggaggaag tagtcttcct tgaagttgtg gaagccctga atcagttcgt
     4441 gaaaattctg catggctggt gcactgaatg aagtgacgac tatcttacgc ctcgcggcct
     4501 caggacgcca agccggcccc ctgatatgaa tccaatggat actgcagggc gcggttgatc
     4561 gcggcgaacc gtgctacgtg caatcgccta ggtctgatgg cctagttctg tcacaaggga
     4621 aattcaggcg gatacactca ttcctgtcga gcggccgacg ggccgcttgt gacgcccggc
     4681 gggagccgga cgcgacaatt cgatcaacac cggaaaaatc cagtagaaca caggaaaagg
     4741 aaccttcggt atgaacatga agaagctcgt cgcagcagca gcgctcgctc ttatggttgt
     4801 cggcactcag gccggagccg aaacactgcg cgtcggcagt gaaaccgtct atcctccttt
     4861 cgaattcctc gactccaact caggcaagta cgtcggcttc gacatcgatc tgatcgacg
//
LOCUS       DBMDTI010000143         4911 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4137_length_4911_cov_2.656757, whole genome shotgun sequence.
ACCESSION   DBMDTI010000143 DBMDTI010000000
VERSION     DBMDTI010000143.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4137_length_4911_cov_2.656757"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="ACZPB6_07165"
     CDS             complement(<1..416)
                     /locus_tag="ACZPB6_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012354.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1184281.1"
                     /translation="MIQSSLSQQDLLFFVTLCRLQSLTVSAQKLNLSLSSACRMLTHL
                     RETFGEPLFVRSRSGLMPTPGAMALLPKAIMLLDGFDDLFEPPVFDPAQAQRLIRIAC
                     ADFGAVSILTKVIPAIIDKAPHLDFQILPIESDLVG"
     gene            766..2592
                     /locus_tag="ACZPB6_07170"
     CDS             766..2592
                     /locus_tag="ACZPB6_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HIR1184282.1"
                     /translation="MNRRKFLSCAVLAATAGATGGAMAMGTASGYNVVGFAALYGPLG
                     EVLVNPYGIAPLTAIIKNGGYELLDADVRVVPKKDGQEIAYKVSRSQLLTHGGVPVFG
                     LYADYQNQVEVTAKKRFKGQVETVKFTYTIYAGPITGIPSGAPHEKSLMFKANVKKVS
                     KKFADRLYFVNNLGTPNAQTMRTIWNNPMGGAMTWQFPPKTVIIDTKGEIRWFLDYRD
                     LWKPEDPYSNGVMMGFHQNPDGCLTFGFGQRYAKYDLMGRKIWNRRLPNAYADFSHAL
                     DPAQNGHYFLRVSSADLRRADEKRVHTVRDVIIEVDQNGTVVDEWRLFDILDPYRSDV
                     IKALDQGAVCLNIDPSKSGHTLSAEELAKQEAEGEFGDIAGVGPGRNWAHVNSVDYDP
                     TDDSIIISSRHQSACIKIGRDKKVKWILGAPKGWRSPWKEALLTPVSAKGQKLNCTDV
                     ECTDTDFDWTYTQHTAFRVDEKSDKRYCMISVFDNGDARNLDQPALASMKYSRAVIYK
                     IDQKNMTVEQVWEYGKERGHDWYSPVTSLTRYEADKDSVMVYSATAGMDFSLSGGSST
                     PPRPVLNEFEWGAKEPAVEIQMSPGSGYRAFPISVKKAFTEN"
     gene            complement(2544..3452)
                     /locus_tag="ACZPB6_07175"
     CDS             complement(2544..3452)
                     /locus_tag="ACZPB6_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013460.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR1184283.1"
                     /translation="MRALRGTTRTGVPLCKRSSGTPRSSASIPPTRSSTSSTSSAGAL
                     AASGIAHGSAARAGIRPERLDVSVHFIAGFALIIVIAAWLVFSHARARRFVHEILRTE
                     RPFLAWLHNLGGYPTKLLRFLRLTKAEPSTAPQGRYNAGQRIAYALLVLLNLTLIGTG
                     VLLFILHQPHEQSPFYQGALAAHSFAFKAGVLILLAHIPMGLLSRTTQDDPAWLGRTH
                     SRRDGRKARPALVPRGPRAPCGRARPSRRAAPLTQGRIPRNVKDLRRWQSPEVLLSAW
                     GRTRPVRMPQDQFSVKAFFTLIGKAR"
     gene            complement(3702..4220)
                     /locus_tag="ACZPB6_07180"
     CDS             complement(3702..4220)
                     /locus_tag="ACZPB6_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR1184284.1"
                     /translation="MGALMSAVMNLLKAADRCVSVTARTVSTLALAGIFVMFIANVFV
                     RFVPVYNFTQTDDWIQILLVWMIFPGAMELVRTRGHFVVDVITDRVAGTNFGHCCRLA
                     VTVIELVTYALICWYGIVWVLRAQATFQSIPWLQVRWAYAAIPVSAFFMTIYGVRDLI
                     LAVRAFLPAKSA"
     gene            complement(4208..>4911)
                     /locus_tag="ACZPB6_07185"
     CDS             complement(4208..>4911)
                     /locus_tag="ACZPB6_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR1184285.1"
                     /translation="SARRNYAKTEPAPWRERLQAFREAFFALMTPVIVMGGMFGGIFT
                     ATEAAAVASLYVMFLGFFVYGTLRVRDLPAIFWNTVEQTAKVMFVIATAGFFQYVLLY
                     TRIPQQAIAFMSTSFATAAPVLLLIIALLVLMGCFMEGTAILLITVPIFVPLAKSFGY
                     DIVQLGIVMCISLAIGVLTPPVGLNLYVMSSITGEHVLAIAKEAVGYVLIMIIVAILV
                     AFIPELSLGFAKWVP"
BASE COUNT         1021 a         1435 c         1673 g          782 t
ORIGIN      
        1 ttgccgacga ggtccgattc gattggcagg atctggaagt cgagatgcgg cgccttgtca
       61 atgatggcgg gaatcacctt cgtgagaatc gaaaccgcgc cgaagtctgc gcaggcgatg
      121 cggatgaggc gctgggcctg cgcgggatcg aagacgggag gctcgaacag gtcgtcgaag
      181 ccgtcaagca gcatgatcgc cttgggcaga agggccatgg cgcccggcgt gggcatgagg
      241 ccgctgcggc tgcgtacgaa gagcggctct ccgaaggtct cgcgaaggtg cgtgagcatg
      301 cggcaggcgg acgacaggct gaggttgagc ttttgggcgc tgacggtcag ggactgaagg
      361 cggcagagcg tcacgaagaa gagaaggtcc tgctggctca gggaggactg aatcatggtg
      421 ctggtcccgg gctgaaaaaa aaacgacgtt cgggtggatg ctccgaactc cttctgcggc
      481 aggtgctttg atagatccat gaggttagca cgaagcgttt ctccggtgtg ggcgagccgg
      541 ttgggaataa cccgaacggc gcgcacgaaa tcgccagatc tgacaaaagg ctcatggccg
      601 aattcgcaaa atggaggagg aatgagctta tggaaggcag aaaacacgct ctgtaacatt
      661 cggaacgaag ggcggcggct tgactcgtcc cgcctcatcc gaccagtgcg tcgggcgttt
      721 cgcggatgcg gacgtccgcg cgacattcac aaaggaatag agaagatgaa ccgcagaaag
      781 tttttgagct gtgccgtgct tgcggcgacc gcgggcgcca cgggcggcgc catggccatg
      841 ggcaccgcct cgggctacaa cgtcgtgggc tttgcggccc tttacggtcc gttgggcgaa
      901 gtgctcgtca atccgtacgg cattgcgccg ctcactgcca tcatcaagaa cggcggctat
      961 gaacttctcg atgccgacgt gcgcgtcgtg ccgaagaagg acggtcagga aatcgcctac
     1021 aaggtctccc gctcccagct cctcacgcac ggcggcgtgc cggtcttcgg tctgtacgcc
     1081 gattaccaga accaggtcga agtgaccgcc aagaagcgct tcaagggcca ggtcgaaacc
     1141 gtcaagttca cctacacgat ctacgccggc ccgatcaccg gcattccgtc cggcgccccg
     1201 cacgaaaaga gcctgatgtt caaggccaac gtgaagaagg tctccaagaa gttcgccgac
     1261 cgcctctact tcgtcaacaa cctcggcacg ccgaacgccc agaccatgcg caccatctgg
     1321 aacaacccga tgggcggcgc catgacctgg cagttcccgc ccaagacggt catcatcgac
     1381 acgaagggcg aaatccgttg gttcctcgac taccgcgacc tctggaagcc cgaggatccg
     1441 tattccaacg gcgtgatgat gggcttccac cagaatccgg acggctgcct cactttcggc
     1501 ttcggccagc gctacgccaa gtacgacctc atgggccgca agatctggaa ccgccgtctg
     1561 cccaacgcct acgccgactt ctcccacgcc ctggatcccg cccagaacgg ccactacttc
     1621 ctgcgcgtct cctccgccga cctgcgtcgc gccgatgaaa agcgcgtgca cacggtgcgc
     1681 gacgtgatca tcgaagtcga ccagaacggc acggtagtcg acgaatggcg tctcttcgac
     1741 atcctcgacc cgtatcgctc cgacgtgatc aaggcccttg accagggcgc cgtgtgcctg
     1801 aacatcgacc cgtcgaagtc cggccacacg ctctccgccg aagagctcgc caagcaggag
     1861 gccgaaggcg aattcggcga catcgccggc gtgggtccgg gccgcaactg ggctcacgtc
     1921 aactccgtcg actacgaccc gaccgacgac tcgatcatca tctcctcgcg ccatcagtcc
     1981 gcctgcatca agatcggccg cgacaagaag gtcaagtgga tcctcggcgc gcccaagggc
     2041 tggcgctctc cctggaagga ggctctgctc acgccggtct ccgccaaggg ccagaagctc
     2101 aactgcacgg acgtcgagtg cacggacacc gacttcgact ggacgtacac gcagcacacg
     2161 gccttccgcg ttgatgaaaa gtccgacaag cgctactgca tgatctccgt cttcgacaac
     2221 ggcgacgccc gcaatctcga ccagccggcc ctcgcctcga tgaagtacag ccgcgccgtc
     2281 atctacaaga tcgaccagaa gaacatgacc gtcgagcagg tctgggaata cggcaaggaa
     2341 cgcggccacg actggtacag cccggtcacg tcgctcaccc gctacgaagc cgacaaggac
     2401 tccgtcatgg tgtactccgc cacggccggc atggacttca gcctctcggg tggctcctcg
     2461 acgcctccgc gtcctgttct taatgaattc gaatggggcg ccaaggagcc cgccgtcgaa
     2521 attcagatga gcccgggcag cggctaccgc gccttcccga tcagcgtgaa gaaggccttc
     2581 acggaaaact gatcctgagg catccgcacg ggcctcgtgc ggccccatgc agaaagaagg
     2641 acctccggcg actgccagcg ccggaggtcc ttcacattcc gaggaatgcg tccctgagtc
     2701 agaggcgccg ctcgacgaga aggcctggct cgcccgcatg gggctcgagg tcctcgcgga
     2761 acaagagcgg ggcgtgcttt gcggccgtct ctgcgggaat gcgtccgccc gagccaggca
     2821 ggatcgtctt gagttgtgcg agagagaagc cccatcggaa tgtgggcgag aagaatcagg
     2881 acgcccgcct tgaaggcaaa actgtgggca gcgagcgcgc cctgatagaa cggcgactgc
     2941 tcatgaggct ggtgcaggat gaagaggagc acgcccgtgc cgatcagcgt gaggttgagg
     3001 agcacgagga gcgcgtaggc gatgcgctgg cctgcgttgt agcggccttg cggcgcggtc
     3061 gagggttcgg ccttcgtgag gcgcaggaac ctgagaagct ttgttggata gccgccgaga
     3121 ttgtgcagcc aggcgaggaa cgggcgctcg gtgcgtagga tttcgtgcac gaagcgcctt
     3181 gccctcgcgt gcgagaagac gagccatgcg gcgatgacga tgatgagcgc gaagcccgcg
     3241 atgaagtgga cggagacgtc gaggcgttcc gggcgtatgc ctgcgcgtgc ggcggatccg
     3301 tgcgcaatgc ctgaggcggc gagcgccccg gcagatgagg ttgaggaagt ggacgatctg
     3361 gtcggaggga tggaagcgct tgaccgaggc gtgcctgacg agcgtttgca taaggggact
     3421 cctgtacggg ttgtgccgcg gagcgccctc aaggcggaat gacggatctg cggcgggttg
     3481 aacgagcggc ggggagcgaa aggcctcccg agcgtgtttt caatcgcgcc acgaggtctt
     3541 ggggcaatgt tggttcagac ccgtgtaaag aagcgcaagg acgtgtcggc aaagtgggag
     3601 gaaatccggg aaggacatgt tgaggtcatg aaaagggcgc ggtccctgcg ggaggcgcgc
     3661 ccatgcgtgc atttgcgaac ggtctgatga gtttgccgcg gtcaggccga cttggcgggc
     3721 aggaaggccc tgacggcgag gatcaggtcg cgcacgccgt agatcgtcat gaagaaggcc
     3781 gagacgggga tggcggcgta ggcccagcgg acctgcagcc aggggatcga ctggaaggtg
     3841 gcctgtgcgc gcaggaccca gacgatgccg taccagcaga tgagcgcgta ggtgacgagc
     3901 tcgatcacgg tgacggcgag gcggcagcag tggccgaagt tcgtgcccgc gacgcggtcg
     3961 gtgatgacgt cgacgacgaa gtggccgcgc gtgcgcacga gttccatggc gccggggaag
     4021 atcatccaga cgagcaggat ctggatccag tcgtcggtct gggtgaagtt gtagacgggc
     4081 acgaagcgca cgaagacgtt ggcgatgaac atcacgaaga tgcctgcgag cgcgagcgtg
     4141 ctcacggtgc gcgcggtgac ggagacgcag cggtcggccg ccttgagaag gttcatgacg
     4201 gcggacatca gggcacccat ttggcgaagc cgagcgagag ctcggggatg aaggcgacga
     4261 ggatcgccac gatgatcatg atgaggacgt agcccacggc ctccttcgcg atggcaagga
     4321 cgtgctcgcc cgtgatcgag ctcatcacgt agaggttgag gcctacgggc ggcgtgagca
     4381 cgccgatggc gagcgagatg cacatcacga tgccgagctg aacgatgtcg tagccgaagc
     4441 tcttggcgag cggcacgaag atcgggacgg tgatgaggag gatggccgtg ccctccatga
     4501 agcagcccat cagcacgagg agcgcgatga tgagaaggag cacgggcgcc gcagtcgcaa
     4561 aggaggtcga catgaaggcg atcgcctgct gcggaatgcg ggtgtagagg agtacgtact
     4621 ggaagaagcc cgcggtggca atgacgaaca tcaccttggc ggtctgctcg accgtgttcc
     4681 agaagatggc gggaaggtcg cgcaccctga gcgttccgta aacgaagaag ccgaggaaca
     4741 tcacgtagag ggacgcgacg gcggccgctt cggtcgccgt gaagatgccg ccgaacatgc
     4801 cgcccatcac gatgacgggg gtcatgagcg cgaagaaggc ctccctgaag gcctgaaggc
     4861 gctcgcgcca gggcgctggc tcggtcttgg cgtagtttcg gcgcgcggag a
//
LOCUS       DBMDTI010000144         4826 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4220_length_4826_cov_4.749630, whole genome shotgun sequence.
ACCESSION   DBMDTI010000144 DBMDTI010000000
VERSION     DBMDTI010000144.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4220_length_4826_cov_4.749630"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="ACZPB6_07190"
     CDS             <1..561
                     /locus_tag="ACZPB6_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR1184286.1"
                     /translation="MRSQPEVLDRAMTNFFAALRAFPKPVVAQVMGPCVGDALSMLLY
                     CDLVYASERALFSLPCVALGQTTRFGSAFIMAAAAGLPRATEKLMLSEPITAAEALEM
                     RIITGIADDESLDNLVASKVARLAVLPPGAVRAMKALLTGPRNKALEVAAEEEEAVWR
                     HQAGSAEAAEALAAFLEGRKPAFRPE"
     gene            851..1747
                     /locus_tag="ACZPB6_07195"
     CDS             851..1747
                     /locus_tag="ACZPB6_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1184287.1"
                     /translation="MKLSLPLTGLLCAGMLLTSAASAAELTGTLKRIQHSGTMTLGYR
                     EASVPFSYLQADGTPTGYGFEVCKAVSEAVKKELNMPNLTIKYQAVTSANRIPLIQNG
                     TVDIECGSTTNSKARQREAGFGINYFGVQVTAAVWKDSGINSIKDLNNKNVAVTSGTT
                     AVALLKKFEKDNGIKIRYLMTKDFAESMQLVAKKRAAAFVIDDVLLAGQISNLPNPQD
                     FKIVDASLSVEPYAPMFAKDDPAFKALVDKTIVGMMKDGTLDKLYKKWFESPIPPKNA
                     NLNFPMNSVTKELFANPNSDGI"
     gene            1842..2576
                     /locus_tag="ACZPB6_07200"
     CDS             1842..2576
                     /locus_tag="ACZPB6_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1184288.1"
                     /translation="MALNMTLNTLWETSLLSDDPWWLYLVYGAGWTISLAVASFALAL
                     TLGTIVGTLRTVDNKWIRGICEGWIELFRNIPLIVQIFIWYFVVPELIPPYKDWMIEA
                     DPVLSQFLSAFLCMGLFTSSRIAEQVRAGITSIPSGLPYAAKALGFTVIQRYRFVILP
                     MAFRLVLPPLTSEAMNLIKNTAVAMTIGLPELTMRANEMGEATFQFFAAYLWATVLYI
                     AIALCVNRLMAWMEARCAIPGFIAAK"
     gene            2592..3275
                     /locus_tag="ACZPB6_07205"
     CDS             2592..3275
                     /locus_tag="ACZPB6_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1184289.1"
                     /translation="MDFTSITNSWGYILSGAAYTVQLTAITAVGGLFLGTLLALCHIS
                     EKRWLSAPAKLYINLMRAVPLVLVLFWYFLLMPEILRIILGLDHPVKIGAEMTAIITF
                     VLFEAAYFAEIMRAGIQSVKKGQIQAAYGLGLSYAQTMRHVVLPQAIRHMLPVLLTQT
                     IILFQDTSLVYVISGNDFMGSISKIAQRDGQLVLMYSFAAVCYLAVSLSLSLLIRRLQ
                     TRMRTAQAH"
     gene            3309..4037
                     /locus_tag="ACZPB6_07210"
     CDS             3309..4037
                     /locus_tag="ACZPB6_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1184290.1"
                     /translation="MIEIKNISKWYGNFQVLKDCSVDVEKGEVVVVCGPSGSGKSTLI
                     KTVNALEPFQKGEIIVDGISVGDPKTNLPQLRSRVGMVFQHFELFPHLSIRENLVLAQ
                     MKVLGRGREEATERGLKLLDRVGLLKHADKFPGQMSGGQQQRVAIARALAMDPICMLF
                     DEPTSALDPEMINEVLDVMVQLAKEGMTMMVVTHEMGFARKVADRVIFMDAGEIIENL
                     PAQEFFNSPHTERAQQFLSKILQH"
     gene            4174..>4826
                     /locus_tag="ACZPB6_07215"
     CDS             4174..>4826
                     /locus_tag="ACZPB6_07215"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR1184291.1"
                     /translation="MSNTHAFDAAAALQLGQEVLTQEAGALTHLASTLGSEFTDAVSR
                     ILSCKGRLIVSGVGKSGHIGRKLAATFASTGTPAYFVHAAEAAHGDLGMITADDVVLA
                     ISYSGETNELLTIVPTLKREGATLIAITGNPNSSLAQHADVHLNCHVSREACPLNLAP
                     TTSTTATLALGDALAVATLSAKGFTKEDFARSHPGGALGRRLLIHVQDIMRTGEDIPA
                     "
BASE COUNT          907 a         1733 c         1240 g          946 t
ORIGIN      
        1 atgcgctccc agcccgaagt gctcgaccgt gccatgacga acttcttcgc cgcgctgcgc
       61 gccttcccga agcccgtcgt cgcccaggtg atgggcccgt gcgtgggcga cgcgctctcg
      121 atgctgctct actgcgacct cgtctacgca tccgaacgcg ccctcttctc cctgccctgc
      181 gtcgcgctcg gccagacgac ccgcttcggc tccgccttca tcatggcagc cgcggcaggc
      241 ctgccccgcg ccaccgagaa gctcatgctc tccgagccga ttaccgccgc cgaggccctc
      301 gagatgcgca tcatcacggg cattgcggat gacgaatccc tcgacaacct cgtcgcctcc
      361 aaggtcgctc gtctcgccgt cctgccgccg ggcgccgttc gcgccatgaa ggctcttctc
      421 accggcccgc gcaacaaggc cctcgaagtc gccgccgaag aagaggaagc cgtctggcgc
      481 caccaggcgg gatccgccga agcggccgaa gcgctcgctg ccttcctcga aggccgcaag
      541 cccgccttcc gtcccgaata atcctccgcc ctttttcggc acccaaagcg cgcactattt
      601 tggtgcgcgc tttgttttgg tgcatcccgt tccgccctcc tttggacccc gtccgccccc
      661 gtcttctcct ccttcaagga acgctcctag tcgcattctg ctcatcgact ctgccgcagt
      721 cgtttcaacg atctaattta tttaccttag cggttttggc acgctgattg ctacgtagaa
      781 gggcaacacc cctgcgaccg ttcgaatttt cacagtccct cgtcagacac aagttttcag
      841 gagttccacc atgaagcttt ccctcccgct caccggcctg ctctgcgcag gcatgcttct
      901 cacttccgcc gcctccgccg ccgaactgac cggcacgctc aagcgcatcc agcactccgg
      961 cacgatgacg ctcggctacc gcgaagcttc cgttccgttc tcctacctgc aggctgacgg
     1021 cacgcccacg ggctacggct tcgaagtctg caaggccgtc agcgaggccg tgaagaagga
     1081 actcaacatg ccgaacctga cgatcaagta tcaggccgtc acgagcgcga accgaattcc
     1141 gctcatccag aacggcacgg ttgacatcga atgcggctcc acgacgaact ccaaggcccg
     1201 tcagcgcgaa gccggcttcg gcatcaacta cttcggcgtt caggtcactg ccgccgtgtg
     1261 gaaggactcc ggtatcaact ccatcaagga tctgaacaac aagaacgtcg ccgtcacgtc
     1321 cggcacgacc gccgtcgccc tgctcaagaa gtttgaaaag gacaacggca tcaagatccg
     1381 ctacctgatg acgaaggact tcgccgaatc catgcagctt gtcgccaaga agcgcgccgc
     1441 cgccttcgtg atcgacgacg tgctgctcgc cggccagatt tcgaacctgc ccaacccgca
     1501 ggacttcaag atcgtcgacg cctcgctctc cgtcgaaccg tacgccccga tgttcgccaa
     1561 ggacgacccc gccttcaagg ctctggttga caagaccatc gtcggcatga tgaaggacgg
     1621 cacgctcgac aagctctaca agaagtggtt cgaatccccg attccgccca agaacgccaa
     1681 cctcaacttc ccgatgaaca gcgtcacgaa ggaactcttc gcgaacccga acagcgacgg
     1741 catctaagcc caagccttta cggtccccgg gacgccagct agcaatcgcg tcccggggac
     1801 cccttttaga tttaattccc gaaaacaaga ggaaccccgc catggcactg aacatgaccc
     1861 tcaacactct ctgggaaacc tccctgctga gtgatgatcc ctggtggctt tatctcgtct
     1921 acggcgcagg ctggaccatc agccttgccg tcgcctcctt tgcgctcgcc ctgacgctcg
     1981 gcaccatcgt cggcactctc cgcaccgtcg acaacaagtg gatccgaggc atctgcgaag
     2041 gctggatcga gcttttccgc aacattcccc tcatcgtcca gatcttcatc tggtacttcg
     2101 tcgtccccga actgatcccg ccctacaagg actggatgat cgaggccgac cccgtcctca
     2161 gccagttcct ctcggccttc ctctgcatgg gcctcttcac gtcgtcccga atcgccgaac
     2221 aggttcgcgc cggcatcacg tccattccct ccggtcttcc ctacgccgcc aaggccctgg
     2281 gcttcacggt cattcagcgc taccgcttcg tcatccttcc gatggccttc cgcctcgtgc
     2341 tgccgccgct cacaagcgaa gcgatgaacc tcatcaagaa cacggccgtg gccatgacga
     2401 tcggcctgcc ggaacttacg atgcgcgcca atgaaatggg cgaggcgacc ttccagttct
     2461 tcgccgccta cctctgggcg acggtcctct acatcgccat cgcgctgtgc gtcaaccgcc
     2521 tgatggcctg gatggaagcg cgctgcgcga tccccggctt catcgccgcc aagtaagcaa
     2581 aggagtcggt catggatttc acttcgatta cgaacagctg gggctacatc ctttcgggcg
     2641 ccgcctacac ggtgcagctc actgcgatca cggctgtggg cggcctcttc ctcggaacgc
     2701 tgctcgccct ctgccacatt tccgaaaagc gctggctctc cgcccccgca aagctctaca
     2761 tcaacctgat gcgtgcggtg cctctggtgc tcgtgctctt ctggtacttc ctgctgatgc
     2821 cggaaattct gcgcatcatc ctcggcctcg accaccccgt gaagatcggc gccgaaatga
     2881 ccgccatcat caccttcgtg ctcttcgagg cggcctactt cgccgagatc atgcgcgcgg
     2941 gcatccagtc cgtgaagaag ggacagattc aggctgcata cggacttggg ttatcctatg
     3001 ctcagaccat gcggcacgtc gtgctcccgc aggccatccg tcacatgctg cccgtgcttc
     3061 tgacgcagac gatcattctc ttccaggaca ccagtctcgt ctacgtcatc agcggcaatg
     3121 acttcatggg ttcgatctcc aagatcgccc agcgtgacgg acagcttgtt ttgatgtact
     3181 cttttgctgc tgtctgctat cttgccgtct ccttgtccct ctccctgctg attcgtcgac
     3241 tgcagacgag aatgcgaacg gctcaggctc actaaaaaat ttgaacagga tatcccccta
     3301 tcatggcaat gatcgaaatc aagaacatca gcaagtggta cggcaacttc caggtgctga
     3361 aggactgctc cgttgatgtt gaaaagggcg aagtcgtcgt ggtctgcgga ccgtccggct
     3421 ccggcaagtc caccctcatc aagaccgtca atgctctcga acccttccag aagggcgaaa
     3481 tcatcgtcga cggcatctcc gttggcgatc cgaagacgaa ccttccccag ctgcgcagcc
     3541 gcgtcggcat ggtgttccag cacttcgagc tcttcccgca cctctccatc cgcgagaacc
     3601 tcgttctcgc ccagatgaag gtgctcggcc gcggccgcga ggaagccacc gagcgcggtc
     3661 tgaagcttct cgaccgcgtc ggccttctca agcacgccga caagttcccc ggccagatgt
     3721 ccggcggcca gcagcagcgc gtggccatcg cccgcgcctt ggccatggac ccgatctgca
     3781 tgctctttga cgaaccgacg tccgccctcg acccggaaat gatcaatgaa gtgctcgacg
     3841 tgatggtcca gctcgccaag gaaggcatga ccatgatggt cgtgacccac gaaatgggct
     3901 tcgcccgcaa ggtggccgac cgcgtgatct tcatggacgc aggcgaaatc attgaaaacc
     3961 tccccgccca ggagttcttc aactcgccgc acaccgaacg cgcccagcag ttcctctcca
     4021 agattctgca gcactgattt ttcagcgctc aggcctcgaa accccgccgc ttcccggcgg
     4081 ggttttgtct ttttccgaac cccctcaggg tcatgccacg ggttatcatt aacaggttgt
     4141 acagcttttt ttcccgcaaa gaaggaccgt caaatgagca acactcatgc attcgatgcc
     4201 gcagcagctc tccagctcgg gcaggaagtt ctgacccagg aagccggggc gctcacgcac
     4261 ctcgcctcga cgctcggcag cgaattcacg gacgccgttt cgcgcattct ttcctgcaag
     4321 ggccgtctga tcgtgtccgg cgtgggcaag tccggccaca tcggccgcaa gctcgccgcc
     4381 acctttgcct ccacgggcac ccccgcctac ttcgtccatg ccgctgaagc cgcccacggc
     4441 gacctcggca tgatcacggc cgacgacgtc gtgctcgcca tctcgtactc cggcgaaacc
     4501 aacgagcttc tcaccatcgt ccccacgctc aagcgcgagg gcgcgacgct catcgccatc
     4561 acgggcaacc cgaactccag cctcgcccag cacgccgatg tgcatctcaa ctgccacgtc
     4621 agccgcgaag cctgcccgct caatctggcg ccgaccacgt ccacgaccgc aacgctcgcc
     4681 ctcggcgacg cgctcgccgt tgcgaccctc tccgccaagg gcttcacgaa ggaggacttc
     4741 gcgcgcagcc atccgggcgg agccctcggc cgccgcctgc tcatccacgt tcaggacatc
     4801 atgcgtacgg gcgaggacat tcccgc
//
LOCUS       DBMDTI010000145         4775 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4275_length_4775_cov_2.221652, whole genome shotgun sequence.
ACCESSION   DBMDTI010000145 DBMDTI010000000
VERSION     DBMDTI010000145.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4275_length_4775_cov_2.221652"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="ACZPB6_07220"
     CDS             complement(<1..289)
                     /locus_tag="ACZPB6_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1184292.1"
                     /translation="MSALILGHKNPDSDSIISAIACAHLYKQRGLDVVPAAQGTPAPE
                     TAFILERFGLEAPEIVTSVAGRDVYLVDYSDKAQAPADIDECNLKGIVDHHK"
     gene            605..1825
                     /gene="hmpA"
                     /locus_tag="ACZPB6_07225"
     CDS             605..1825
                     /gene="hmpA"
                     /locus_tag="ACZPB6_07225"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HIR1184293.1"
                     /translation="MLTPRQIEQVKATVPVLREHGVLLTTHFYRRMLEGNPELRNIFN
                     QAHQARGRQQKALAEAVLAYAENIENPAVLLPAVKFIASKHATVGIRAEHYPIVGRHL
                     LASIREVLGEAASDELLEAWGAAYGQLADLMIEVESGIYEAQANADGGWSGWRPFIVS
                     RRVEETPDVVSLYLTPADGGKVPVWEPGQFVSVRAYLPELKLVQPRQYSLSAAPEDRS
                     QLRISVKRIAAAGDAPAGLMSNHLHKCLKAGDTIDVSAPAGEFHLAEGTNPVFLAGAG
                     IGVTPIFAMLESIARNTPERQVTFVQVARTASELVFVNEVREAAGKLKNVKLLAFVTQ
                     PAEADTTIKCPCVKLGARPSVEDIRALAPSADVDAYLCGPGDFMSGMRAALEAAGVPA
                     RQIHAEVFGTGSEA"
     gene            1970..2491
                     /locus_tag="ACZPB6_07230"
     CDS             1970..2491
                     /locus_tag="ACZPB6_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014173.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1184294.1"
                     /translation="MHLKRFTDLGLRILMHLANEADPETPHSVPELSRFLCWNQNLVI
                     KTANAMVKAGWLSAVRGRHGGLRLACKPEDLRIGDVVRTLEDNDMLVDCNEPPCPFAG
                     RCVLIDALARAREAFYKELNQYTLADLRRSGPGNGVSNLMCSRAQTISDEPADDPGGS
                     EHRMEQASVRQQA"
     gene            2615..3109
                     /locus_tag="ACZPB6_07235"
     CDS             2615..3109
                     /locus_tag="ACZPB6_07235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024304.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR1184295.1"
                     /translation="MNHTNLHPMRRPDRAASEDFARRTANEAPYGTLVTASASGEPYG
                     TPVSPVIEDDKIYFHAAAGAGRKFENLTSNPRCSMVFVSMTEPDEPAFSMNYRSAVFE
                     GRATLVTDEIEKMHALWLIGARWAGSQPIAKRRAYIEGDMASCDVWRIDVDVISGKTR
                     EMRR"
     gene            complement(3123..4442)
                     /locus_tag="ACZPB6_07240"
     CDS             complement(3123..4442)
                     /locus_tag="ACZPB6_07240"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1184296.1"
                     /translation="MHEILFPSPFGTPPSPELVARARLAPVPAGFTGRAGVLVFEDEA
                     GVHAEHAFEPVSRALCGAGGEGRDWSTLRAAALWPGMSRDEVPERTLVDGRGVRFTVR
                     DFLKAMFEPYPDLPEMQGGRELEARKAGWRMAVTDALTSPVVALVAARNEAAVRGRIS
                     GNARIIGLAEWWCGPTPAHEIRFDGTFYPPRSAAKWLYERLVEGLPAAPVPAIASEED
                     FETLPVLYFDDDIVVIDKPTRLASVPGVRETTSAMEILEKQFGPLRIVHRLDMDTSGV
                     LLFARSLRAEKALHESFREGLAMKRYVARLEGVPEQMSGRIELPLALNRLDRPRQCVL
                     SEAEGGKPAATDWELLRVETMPDGRRKALVSLWPETGRTHQLRVHCAHAAGIGLPIDG
                     DSFYGSRGILAEEKTSRLCLHAAELTIPHPTTGIPVHFEAAEAFPKF"
BASE COUNT          906 a         1540 c         1477 g          852 t
ORIGIN      
        1 gcttgtgatg gtcgacgatg cccttgaggt tgcactcatc aatgtcggcg ggagcctggg
       61 ccttgtcgga gtagtcgacg aggtagacgt cgcggccggc gacgctcgtc acgatttccg
      121 gagcttcaag gccgaagcgc tcgaggatga aggccgtttc cggggcgggc gtgccctggg
      181 cggccggcac gacgtccaga ccacgctgct tgtagagatg cgcgcaggca atggcggaaa
      241 tgatgctgtc ggagtcgggg ttcttatgac caagaatgag agcggacact ttgaggcctt
      301 tctaaaaagg ggatgaataa aaattccgga tattgtagcg gactttgagc ggctcgggcg
      361 ggctagccgc acaggtccgg ccctctgccg acggcggccg agtcttgcta cgcaaattcg
      421 acgatgcgaa cgcgaagcat tctcttacaa aaggcgacgc gcgcttcttg acaatacaga
      481 tatttgaaat ataagatttg ttcaaccaac atcgaacggg cccgtgacga tgtcagtccg
      541 catccgccgc caggcgagat gcgcttcacc gacaagaatt ccattgcaac tgggagacca
      601 atccatgctg acaccccgac agatcgaaca agtcaaggcc accgtgccgg tacttcgtga
      661 acacggcgtt ttgctcacaa cgcacttcta ccgccgcatg ctcgaaggaa atcccgaact
      721 tcggaacatt ttcaaccagg ctcatcaggc ccgcggacgt cagcagaagg cactggctga
      781 agccgtcctc gcctacgcag aaaacatcga aaatccggcc gtgcttctgc ccgcggtcaa
      841 gttcatcgcc tcgaagcacg caacggtcgg catccgcgcc gagcactatc cgattgtcgg
      901 ccggcatctt ctggcctcca tcagggaggt tctcggagaa gcggcctccg atgaactcct
      961 cgaagcatgg ggtgccgcat acgggcagct cgccgacctg atgatcgaag tcgagagtgg
     1021 catctacgag gctcaggcga acgccgacgg cggctggtct ggctggcgcc ccttcatcgt
     1081 ctcgagacgc gtcgaagaga cgcccgacgt cgtgagcctt tacctgacgc ctgcagatgg
     1141 cgggaaggtg cctgtctggg aacccggcca gttcgtgtcg gtgcgcgcct atctgcccga
     1201 attgaagctc gtccaaccgc gccagtattc gctctcggcg gcaccggagg accggagcca
     1261 gctccgcatt tccgtcaagc gcattgccgc cgcgggcgat gcccccgccg gcctcatgtc
     1321 caatcatctg cacaagtgcc tcaaggccgg cgacaccatc gacgtctcgg cacccgccgg
     1381 cgaattccat cttgccgaag gtacgaatcc ggtgttcctc gccggagccg gcatcggcgt
     1441 cactccgatt ttcgccatgc tcgaatcgat cgcacgaaac acgcccgagc gtcaggtgac
     1501 cttcgttcag gttgcccgca cggcgtctga actcgtcttc gtgaatgagg ttcgtgaggc
     1561 tgccggcaag ctgaagaacg tgaagctgct tgcattcgtg acacagcctg ccgaagccga
     1621 cacgaccatc aagtgtccgt gcgttaagct cggtgcccgt ccgagcgtgg aagacatcag
     1681 ggcgcttgca ccgtcggccg acgtcgacgc ctatctctgc ggcccgggcg acttcatgtc
     1741 agggatgcgc gccgctctcg aagctgctgg cgttccggca cggcagatcc acgctgaagt
     1801 gttcggaacg ggcagcgagg cctgatccga attctgcgga ccgctttcga tccctttaag
     1861 ggatcgggag ccgtccaggc gaaggccatg gacgctgccg tccatcgagt atgatacggg
     1921 aatctcctca cggaaattcc cgttttctca tcctttctcc gaacggacca tgcacctgaa
     1981 gcgatttacc gaccttggcc tgagaattct gatgcacctt gccaacgagg ccgaccccga
     2041 gacgccccat tcggtgccgg agctctcgag attcctctgc tggaaccaga atctggtcat
     2101 caagaccgca aacgccatgg tcaaggccgg gtggctttcc gccgttcgcg gccggcacgg
     2161 cgggctcagg ctggcctgca aacctgaaga cctgcgaatc ggcgacgtcg tgcgcacact
     2221 cgaagacaat gacatgctcg tcgactgcaa cgagccgccc tgtccctttg ccggcagatg
     2281 cgtgctgatc gatgcgctcg ccagagcccg ggaagccttc tacaaggaac tcaaccaata
     2341 cacgctcgcc gatctccgcc gcagcgggcc ggggaatggc gtttcgaacc tgatgtgctc
     2401 gagggcgcaa accatttccg acgagccggc cgacgacccg ggcgggtcag aacaccggat
     2461 ggaacaggcc tcagtcaggc agcaagcatg agacgcacgc cgggacggcg ctgccgtcgg
     2521 agggcttcag tggctcgttt ctgacgtgca ggaacgggca ctcaagtcgc ggataaaatg
     2581 acggtaccca tgttgtccag aaggagctcc gaccatgaat cacacaaact tgcatcccat
     2641 gcgccgccct gaccgcgcgg cctccgagga ctttgcccgc cgcaccgcga acgaggctcc
     2701 ctacggcacg ctcgtgacgg cgtccgcctc gggcgaaccg tacggcacgc ccgtctcgcc
     2761 cgtcatcgag gacgacaaga tctactttca cgcggcggca ggtgcaggtc gaaagttcga
     2821 gaacctcacc tcaaacccgc gctgctcgat ggtcttcgtc agcatgaccg agcccgacga
     2881 accggccttt tcgatgaact accgctctgc ggtcttcgag gggcgcgcga cactcgtcac
     2941 ggacgaaatc gagaagatgc acgcgctctg gctcatcggc gcgcgctggg cgggaagcca
     3001 gcccattgcg aagaggcgtg cctacatcga gggcgacatg gccagctgcg acgtctggcg
     3061 catcgacgtc gacgtcatct ccgggaagac ccgcgagatg aggcgctgaa cagagccttt
     3121 tctcagaact tcgggaacgc ctcggccgcc tcgaagtgca cgggaatgcc cgtcgtcggg
     3181 tgaggaatcg tgagctcggc cgcatggagg cagagtcgcg aggtcttctc ctcggcgagg
     3241 atgccgcggc tgccgtaaaa gctgtcgccg tcgatcggaa ggccgatgcc tgcagcgtgc
     3301 gcgcagtgca cgcgcagctg gtgcgtgcgg cccgtctcgg gccagaggct cacgagggcc
     3361 ttgcgcctgc cgtcgggcat cgtctcgacg cgcagaagct cccagtcggt ggcggcgggc
     3421 ttgccgccct cggcttcgga gagcacgcac tggcgcggac gatcaagcct gttgagggcg
     3481 agcggaagct cgatcctgcc cgacatctgc tcggggacgc cctcgagcct cgccacgtag
     3541 cgcttcatcg caagtccctc gcgaaagctc tcgtgaagcg ccttctcggc cctgaggctt
     3601 ctcgcaaaga gcagaacccc cgaggtgtcc atgtcgagcc tgtgcacgat gcgcagcggt
     3661 ccgaactgct tttcaagaat ctccatggcg gaggtcgtct ccctcacgcc cggcactgag
     3721 gcgagcctcg tcggcttgtc gatcacgacg atgtcgtcat cgaaataaag caccggaagg
     3781 gtctcaaaat cctcttcgga cgcgatggcc ggcacgggcg cggcggggag gccttcgacg
     3841 agacgctcgt agagccactt cgcggcggac ctcggcgggt agaaggtccc gtcaaatcgt
     3901 atttcgtgcg cgggcgtcgg gccgcaccac cattcggcaa gcccgatgat cctcgcgttc
     3961 cccgagatcc gcccccggac tgcggcttcg tttcgtgccg caacgagcgc cacgacgggg
     4021 ctcgtgagcg catccgtcac cgccatgcgc cagcccgcct ttctggcctc gagctctcgc
     4081 ccgccctgca tttcgggcag atcggggtag ggctcgaaca tcgccttgag aaagtccctc
     4141 accgtaaacc tcacgccgcg tccgtcgacg agcgtgcgct cgggcacctc gtccctcgac
     4201 atcccgggcc agagcgccgc cgccctgagg gtcgaccagt cgcggccttc gccgcccgca
     4261 ccgcacaagg cccggctcac gggctcgaag gcatgctcgg catgcacgcc cgcctcatcc
     4321 tcgaagacaa gcacgcccgc acgtcccgtg aagcccgcag gaacaggggc gaggcgcgcc
     4381 cgggcgacga gttcgggcga aggcggggtg ccgaacgggg agggaaagag gatttcgtgc
     4441 atggctcaag ccttcatcga ttgtgggagg aatgtcggga ttctacggaa tcctcggcgc
     4501 accgcggaaa atgagacatg ttaggcagta gcatctaggg gtttttacgt atttcgttcg
     4561 agttttgcgt gtgcgcgaac gccgaaggcg gggagcgcac gctatagttt tttcctttcc
     4621 ctcgtccgat gcggcgtcag accgcaggat cgagggccgc gcgggtgcgt ctgacgcccg
     4681 ttctcggacc cccaccatca cacaggagca gctttccatc atgaccaagc gtcttagccg
     4741 cgtcttccgg tttgccgagc cgatcgttca cggct
//
LOCUS       DBMDTI010000146         4656 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4406_length_4656_cov_2.454885, whole genome shotgun sequence.
ACCESSION   DBMDTI010000146 DBMDTI010000000
VERSION     DBMDTI010000146.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4406_length_4656_cov_2.454885"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="ACZPB6_07245"
     CDS             complement(<1..1271)
                     /locus_tag="ACZPB6_07245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184297.1"
                     /translation="MKKILVATAVAAAFGVSGVQAAEDITVDVAVIGAGGAGLSAAVE
                     AANLGAKVVVVEKMGMPGGNTIRAAGGLNAAGTALQQAKGTKDSVEIAYFDTMKGGHW
                     KNDPELVRTLVSGAAGSVEWLLALGGDLRDVGLMAGASQPRTHRPTGGALVGPEVVKT
                     LYNAAKARQIDMRMNTQATKILMKDGKVTGIQVTNKKDGTYTIHAKAVVDAAGGFGAN
                     NDLVSKYVPRLKGFATTNHPGATGDGLVLAEKAGAQLIDMTEIQTHPTVVPKVGEMIT
                     EAVRGNGAILINKDGKRFFNELNTRDAVSAAILKQKDGVAYLFFDSDMQKSLKATNKY
                     IKQSYCVSGATIEEVAGKMGVPAAELQASIDAWKKGKAAGKDAFGRADMPRNLDQAPF
                     YAIMVTPAVHHTMGGIKIDSKTQVYNTKGQVV"
     gene            1875..2717
                     /locus_tag="ACZPB6_07250"
     CDS             1875..2717
                     /locus_tag="ACZPB6_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR1184298.1"
                     /translation="MHKIPTDAFDMLVTLEKRPFERAGREVVVRLLDETLPDGTKLVA
                     ASDPSYPPELAALEEHPAVLVHEGDLSLTERQLRVSIVGSRDASDSARADARRVAREL
                     AARGAVVVSGLAAGIDTAAHEGALEARSPSGTVGRTIAVMGTPLSQPWPPANARLARR
                     IAEYGLLISLAPQVNGLSFTDEERAESLRARNRAIAALGLGSLVMAGKEGSSTLREVA
                     FSLELGHPVLLWHSIVEEGEPWAIRLLENPPLDSQGRPLVQTVSSAADVEEALSPWST
                     VWWL"
     gene            3114..3734
                     /locus_tag="ACZPB6_07255"
     CDS             3114..3734
                     /locus_tag="ACZPB6_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR1184299.1"
                     /translation="MTFSVWLTFLGASLLLSAAPGPDNLFVLAQSAVYGVRAGVTVVF
                     GLMTGLVLQTFFAACGLAAVVAAVPALFMAIKLAGAAYLLYLAWGAWTHAADPVGTER
                     AVELSPVQLWRRGLIMNITNPKVQIFFLAFFPQFVAPGTTGWALVFQMVVQGLTFILA
                     TFVVFSAIAWAAGAAAKKLQSPAFSLWLNRGSAVLFLALAVFTVLS"
     gene            complement(3851..4300)
                     /locus_tag="ACZPB6_07260"
     CDS             complement(3851..4300)
                     /locus_tag="ACZPB6_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015378.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR1184300.1"
                     /translation="MKIKNTTAAILAAAAMTAATTAWAVDAAPASVPQITENAQPQVG
                     MSAATATAEEAYGGQTRSARLHMSREYGPVLDVRVVKDDGTNVHAFVDAQTGKVVAAD
                     EFGIGEGRHMHDGRGYGHRDHDRGDCGFGFGHGFGHGFGFHGGRACW"
BASE COUNT          782 a         1439 c         1494 g          941 t
ORIGIN      
        1 acgacctggc ccttggtgtt gtagacctgg gtcttggagt cgatcttgat gccacccatg
       61 gtgtggtgga cggccggcgt gaccatgatg gcgtagaacg gagcctgatc gaggttgcgc
      121 ggcatgtcgg cgcggccgaa ggcgtccttg ccggcagcct tgcccttctt ccaggcgtcg
      181 atggaggcct ggagttcggc ggcgggaacg cccatcttgc cggcgacttc ttcaatcgtg
      241 gcgccggaga cgcagtagga ctgcttgatg tacttgttcg tggccttcag ggacttctgc
      301 atgtcggagt cgaagaagag gtaggccacg ccgtccttct gcttgaggat ggcggcggag
      361 accgcgtcgc gggtgttgag ctcgttgaag aagcgcttgc cgtccttgtt gatgaggatg
      421 gcgccgttgc cgcgaacggc ttcggtgatc atttcgccga ctttcggcac cacggtcggg
      481 tgcgtctgga tttcggtcat gtcgatgagc tgggcaccgg ccttttcagc gaggacgagg
      541 ccgtcgcccg tggcgcccgg atggttcgtc gtggcgaagc ccttcaggcg gggcacgtac
      601 ttggagacga ggtcgttgtt ggcaccgaag ccgccggcgg cgtcaacgac ggccttggcg
      661 tggatcgtgt aggtgccgtc cttcttgttc gtgacctgga tgcccgtgac cttgccgtcc
      721 ttcatgagga tcttcgtggc ctgcgtgttc atgcgcatgt cgatctggcg ggccttggcg
      781 gcattgtaga gggtcttgac gacttcagga ccgacgaggg cgccgccggt cgggcggtgc
      841 gtgcggggct gggaggcgcc ggccatcagg ccgacgtcgc gcaggtcgcc gccgagggcg
      901 aggagccatt cgacggagcc ggcagcgccg gaaacaagcg tgcgaacgag ttcgggatcg
      961 ttcttccagt gaccgccctt catcgtgtcg aagtaggcga tttcaacgga gtccttcgtg
     1021 cccttggcct gctgcagggc cgtgccggca gcattgagac cgccagcggc gcgaatcgtg
     1081 ttgccgccgg gcatgcccat cttttcaacg acgacgacct tggcgccgag gttggcggct
     1141 tccactgcgg cggaaaggcc tgcgcctccc gcgccgatca cggcgacgtc gacggtgatg
     1201 tcttcggcag cctgaacgcc ggaaacgccg aaggcggcgg cgacagcagt ggcaaccaga
     1261 atcttcttca tggaaagttc tcctatctca agtcttccga acccccgccg gtcttcttcg
     1321 gacctctcct agcgggcatt cgggaaattg tgtgccggac aacaggtccg gctttttttc
     1381 cagccctaaa tatagaggag agtcccatgt taagacgccc tgaaatttgc cgtaacgcaa
     1441 acgtttgcaa taaagcgcac tgagagaggg aagaagagga aaaaagtgcc gctttcaata
     1501 ggttgaaata cccatataga ggtaggtgag atcccttatt taaatgggcg ggcggcgggt
     1561 ggaatgatgt cggcgcgagg agcttgcgca gaatgcgggt gcatttgatg atagggggaa
     1621 acgtggtcag gtataccctc tgagtggtag tgttgatggg gctttccccg agtgggtaaa
     1681 cccgtttggc aaaagaccca atggtactga tcggacgggg cccttatgat gaactgcatg
     1741 aagacggaca gtcgagggcc ggccaatgcc gcgcgtcccc gcaaaaacgg cgtttcaggc
     1801 atgtgttccg gaccagctgc ccccaactag gcctttcatc ggcgttctgc tccccgatcc
     1861 gtcaaggaga aaccatgcac aagatcccca ccgacgcctt tgacatgctc gtcacgcttg
     1921 aaaagcgccc ctttgaacgc gcgggtcgcg aagtggtcgt gcgtcttctc gacgagacgc
     1981 tccccgatgg cacgaagctc gtcgccgcct ccgatccctc ctatccgccc gagcttgccg
     2041 cccttgagga gcatcccgcc gtgctcgtgc acgaaggcga cctgtcgctc accgagcgcc
     2101 agctgcgcgt ctcgatagtg ggttcgaggg atgcgtccga ctcagcccgc gccgatgcgc
     2161 gtcgcgtcgc ccgcgagctt gccgcacgcg gggccgtcgt cgtctcgggg cttgccgcag
     2221 gcatcgacac cgccgcccac gagggcgcgc tcgaggcgag gagtccgtcg ggcacggtcg
     2281 ggcgcaccat cgccgtcatg gggacgccgc tctcgcagcc ctggccgccc gccaacgcgc
     2341 gccttgcgcg ccgcatcgcc gaatacggcc tgctcatctc gctcgcccct caggtgaacg
     2401 gactctcgtt cacggacgag gagcgcgcgg agagccttcg cgcacgcaac cgcgcgatcg
     2461 cggccctcgg gctcggaagt ctggtgatgg cgggaaagga gggctcgagc accctgcgcg
     2521 aggtcgcgtt ctcgctcgag cttggacacc ccgtgctgct gtggcactcg atcgtcgagg
     2581 agggcgagcc gtgggcgatc aggcttcttg agaatcctcc cctcgattcg cagggacgcc
     2641 cgctcgttca gacggtttcg tccgccgcgg acgtggagga ggcgctgtcg ccctggagca
     2701 ccgtctggtg gctttgatgg gaggacaagg agggaagagg acggggcctc gccggaggtc
     2761 cctgaatcgg aagggaaaaa tccattttac tatgaagaac ctgcagtaat aaacccgtcc
     2821 gttggccttt cctgacgtgc cgcaagaacc gttcaaagaa ggggcttgag tgcggatgct
     2881 tgttctatga tgattcaagc aagcacttgc gcttctctct cgagctcccg tgttgcatca
     2941 ttacctcctg gctcaaccgg tgaagctcgc gcaatatgaa gcttgctgtt tccccgcagt
     3001 gcggcaggcc caagcctctt cccgtagccc gctgcggttt tttttgtccc gtgcatttcc
     3061 gcacggcccg ccaaaacaca acaacactgc acaacgcaca acaggccctc atcatgacct
     3121 tctccgtctg gctcacattc ctcggcgcga gcctgctgct ttctgccgct cccggtcccg
     3181 acaatctctt tgtgctcgcg cagagcgccg tctacggcgt gcgagcgggc gtcaccgtcg
     3241 tgttcggcct catgaccggt ctggtgctgc agacgttctt cgcggcctgt ggccttgcag
     3301 ccgtcgtcgc ggccgttcct gcactcttca tggcgatcaa gctcgcgggc gccgcctatc
     3361 ttctttatct tgcctggggt gcatggaccc acgcggccga tcccgtcggc acggagcgcg
     3421 ccgtcgagct ctcgcccgtg caactctggc gccgcggcct catcatgaac atcacgaacc
     3481 cgaaggtgca gatcttcttc cttgccttct tcccgcagtt cgtcgcaccc ggcacgacgg
     3541 gctgggctct ggtctttcag atggtcgtgc agggcctcac cttcatcctc gccaccttcg
     3601 tcgtcttctc ggccatcgcc tgggcggcgg gcgctgcggc gaagaagctg cagagccccg
     3661 cattttcgct ctggctcaac cgcggcagcg ctgtgctctt cctcgcgctc gcggtcttca
     3721 cggttctctc ctgagggcgg gttttccaaa gccttcgttc ccaaaagcaa agaacgggag
     3781 ccggctccga gtccgctccc gttcttcatc cctgtatttc gggcgcctca tcgtgcgccg
     3841 gaatcattca tcaccagcag gcgcggccgc catggaaccc gaagccgtga ccgaagccgt
     3901 gaccgaagcc gaagccgcag tcgccccggt cgtgatcgcg gtggccgtat ccgcggccgt
     3961 cgtgcatgtg gcgaccttcg ccgatgccga attcgtcggc ggcgaccacc ttgcccgtct
     4021 gcgcgtccac gaaggcgtgg acgttcgtgc cgtcgtcctt caccacgcgg acgtccaaga
     4081 cgggaccgta ttcgcggctc atgtgcaggc gggcggagcg ggtctgtccg ccgtaggcct
     4141 cttcggccgt agcagttgcg gcgctcatgc caacctgcgg ctgcgcgttt tccgtgatct
     4201 gcgggacgga tgcgggggcg gcgtcgacgg cccaggcggt ggtggcggcc gtcatggcgg
     4261 cagcggcgag aatggcggca gtggtgttct tgatcttcat tgtcaaaacc tcttcaaaca
     4321 gttgcagttg ccggtgcgcg ggagagcggc tcgcttgccg cggcgcactg ttcgaatcgg
     4381 actgaagtct aagaagtgaa aattagcgca gacatgggcg gaactgagga cttcctatgt
     4441 cagtgcaacg aatttttcag acaaaaaggc acccgcaggc gtcttgtcct gcgggtgcct
     4501 caaaaagata tctcggatcg ggagatcctg agaaggactt gtgcgggcgt cagatgttgc
     4561 gctcgctcat cataccggag aagtagtcat gcacaccacc tgcaaagatg gtgccgaagg
     4621 tgatccacag gaacaccacg ggaccccaca gagcac
//
LOCUS       DBMDTI010000147         4647 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4415_length_4647_cov_2.761109, whole genome shotgun sequence.
ACCESSION   DBMDTI010000147 DBMDTI010000000
VERSION     DBMDTI010000147.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4415_length_4647_cov_2.761109"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="ACZPB6_07265"
     CDS             complement(<1..275)
                     /locus_tag="ACZPB6_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184301.1"
                     /translation="MNKPCTFLLSALMASAVLAPAAASAADKSELEVRMEAAQKVLAD
                     RSYYERWSPEAALKTVEDAKAANEAGSARQKEIDGALDVMKDWCHTR"
     gene            complement(510..1157)
                     /locus_tag="ACZPB6_07270"
     CDS             complement(510..1157)
                     /locus_tag="ACZPB6_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1184302.1"
                     /translation="MDIVELVRTRYTTKHYDPARRISDRDIADLMEVLRLSPSSVNSQ
                     PWHFFVTGTDEGKARVMPAVLDFNRPRVADASHLVVFAVPVEFTEERFRRLVAQELAD
                     GRVTTEDAASGAADAGRRHFVGLHQVTQEELLSWETRQAYIAMGFLLWAAAERGIDST
                     CLEGLDMAKMDECLGLAEKGLRTVCAVSLGYRLPDDSNAARPKSRLPMDEVVTVF"
     gene            complement(1362..2903)
                     /locus_tag="ACZPB6_07275"
     CDS             complement(1362..2903)
                     /locus_tag="ACZPB6_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR1184303.1"
                     /translation="MEFLLDPTIWVGLLTLVVLEIVLGIDNLVFIAIIAKKLPASQQD
                     KALYVGLGLALAMRLGLLSIMSWLVALTDPVFSVWGHPFSFRDLILLGGGLFLLFKAT
                     TELHERLEALPHSEKHGSGRAGFWVVIAQILLLDAVFSLDSIITAVGMVDNLYVMMSA
                     VVIAMIVMIAASRPLTDFVNRHPTVVVLCLSFLLMIGCSLIAEGLGFHIPKGYLYAAI
                     AFSILIEFFNQLAARNASRNLDRVPFRERTTDAIVRLMSRQPRRDDEEATEDRVVHES
                     FGREERQMVEGVLTLADRNIKTIMTPRSEIAWLNSRRSFEENMKRVQDMPHSSFPVCD
                     GTLENVIGVVKAKDLIGMEPSPEALVRCASERQPLTVPETINVIRLMQDIKSSQASLV
                     LVTDEFGVIQGLTTSHDILEAIAGDFPDEGDRLSIEAVEGGWSAEGSVELFLVEQTCG
                     VDGLMSVDGEYVTLAGLLLDRMGRLPAKGDRLEEAGLVFEVTDVSRHRIERVRITRSP
                     VPAEA"
     gene            3080..3595
                     /locus_tag="ACZPB6_07280"
     CDS             3080..3595
                     /locus_tag="ACZPB6_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024861.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR1184304.1"
                     /translation="MPLTERLSSRVAAEALALAGSEPRGVPAVIDTNVVLDLIYWEDP
                     HSEDLRRALEEGRIHALRSGEAVMELAEVLSRPAFTGDEDEAARLAALWCSMSVPVDE
                     EKIREAADVITVQCRDPLDQKFFTLTLAGGAKLLVSKDKLVLKAGRKMQRYGACAFRP
                     EETAARLDALS"
     gene            4191..>4647
                     /locus_tag="ACZPB6_07285"
     CDS             4191..>4647
                     /locus_tag="ACZPB6_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HIR1184305.1"
                     /translation="MDQTTQIIQSINSLLWGMYGLIPLLVGTGLYFTFKLRFVQIKRF
                     VMAARHVFGGITLFGRKADKDGMSSFQSLATAISAQVGTGNLAGVATAIAMGGPGAIF
                     WMWIAAFFGMATIFSEAVLAQLYRTRDAHGHLTGGPAYYISQGLGSKTLA"
BASE COUNT          902 a         1477 c         1477 g          791 t
ORIGIN      
        1 aagcgcgtgt ggcaccagtc cttcatgacg tcgagcgcac cgtcgatctc cttctgcctg
       61 gccgaacccg cctcattggc ggcctttgca tcctcaaccg tcttcagggc ggcctcgggc
      121 gaccagcgct cgtagtagga gcggtcggcg agcaccttct gcgccgcctc catgcggacc
      181 tcaagctcgg acttgtcggc ggccgaagcg gcggcaggcg ccagcacggc ggacgccatc
      241 agagcggaaa gaagaaaagt gcacggcttg ttcatcggga gaagccctcg tgaagtcttg
      301 attgctgatt gaatcggaca gcccttgcag ggcggggccc gcagccgtcc ggaagtggtg
      361 acagtctacc aaatgcaggc gcgcagacag gccctgcgcc cgcattcccg cccaagacgg
      421 ctcggcccgc attttccggg agaaagtgcg ggccgaaaga ccggatcggg tcgggtcggg
      481 cgcgggcgcg cccagcggac tcccgtgcgt cagaagacgg tcacgacctc gtccatgggc
      541 aggcgggact tcggacgggc ggcgttgctg tcgtcgggca ggcggtagcc gaggctcacg
      601 gcacagacgg tgcgcaggcc cttttctgcg agtccgaggc attcgtccat cttcgccatg
      661 tcgaggccct cgaggcaggt cgagtcgatg ccgcgctcgg cggcggccca gagcaggaag
      721 cccatggcga tgtaggcctg acgcgtctcc caggaaagca gctcctcctg cgtgacctga
      781 tgcaggccca cgaagtggcg gcgtcccgca tcggcggcac ccgaagccgc atcctcggtc
      841 gtcacgcggc cgtcggcgag ctcctgggcg acgaggcggc ggaagcgctc ctcggtgaac
      901 tccacgggca ccgcgaagac gacaagatgg ctcgcatcgg ccacgcgcgg gcggttgaag
      961 tcgagcacgg ccggcatcac gcgggccttg ccttcgtccg tgcccgtcac gaagaagtgc
     1021 cagggctggg agttgacgga ggacggcgac aggcggagaa cctccatgag gtccgcgatg
     1081 tcgcggtcgc tgatcctgcg ggccggatcg tagtgtttgg tcgtgtagcg cgtgcgcaca
     1141 agctcgacga tgtccatgat gtttacctct tttgaaaagt gccgcaggga ggggctcccg
     1201 acggcctgtt ttccattcta tgacgaaagt cgcctcgcgc agagtccctg caagaagcgg
     1261 ttgcaatcgg ccggaaggat cagtccgaaa ggatgaggcg gatgtgcaaa acgcggcccg
     1321 ttctgaacgg agccgcgttt ttcgcgtggt gtcgggcgcc cttacgcctc ggcgggcacg
     1381 gggctgcggg taatcctcac tcgctcgatg cggtggcgcg agacgtcggt cacctcgaag
     1441 acgaggcccg cctcctcaag cctgtccccc ttggcgggga gacggcccat cctgtcgagg
     1501 agaagacccg cgagcgtcac gtactcgccg tcgacgctca tcaggccgtc gacgccgcag
     1561 gtctgctcga cgaggaagag ctcgacgctg ccctcggcgc tccagccgcc ttcaaccgcc
     1621 tcgatcgaga gcctgtcgcc ctcgtcgggg aagtcgccag cgatggcctc gaggatgtcg
     1681 tgggaggtgg tgaggccctg gatcacgccg aactcgtcgg tgacgagcac gagcgaggcc
     1741 tgggagctct tgatgtcctg catgaggcgg atgacgttga tcgtctcggg cacggtgagc
     1801 ggctggcgct cggaagcgca gcgcacgagc gcctcgggcg agggctccat gccgatgagg
     1861 tccttggcct tgacgacgcc gatcacgttc tcgagcgtgc cgtcgcagac ggggaagctg
     1921 ctgtgcggca tgtcctgcac gcgcttcatg ttctcctcga agctgcggcg ggagttgagc
     1981 caggcaatct cgcttctcgg cgtcatgatg gtcttgatgt tgcggtcggc gagcgtgagc
     2041 acgccctcga ccatctggcg ctcctcgcgc ccgaagctct cgtgaacgac acggtcctcg
     2101 gtcgcctctt catcatccct gcgcggttgg cgagacatca ggcgcacgat ggcgtccgtc
     2161 gtgcgctcgc ggaacggcac gcggtcgagg tttctcgagg cattgcgcgc ggcaagctgg
     2221 ttgaagaatt cgatgaggat cgagaaggcg atcgcagcgt agagatagcc cttcggaatg
     2281 tggaagccga gcccctcggc gatgaggctg cagccgatca tcagcaggaa cgagaggcag
     2341 agcaccacca ccgtcggatg acggttgaca aagtccgtga gcggacggct cgcggcaatc
     2401 atcacgatca tggcgatcac gacggcactc atcatcacgt agaggttgtc gaccatgccg
     2461 acggcggtga tgatggagtc gagcgagaag accgcgtcaa gaagcaggat ctgggcgatg
     2521 acgacccaga agccggccct gccgctgccg tgcttttccg agtgcggcaa cgcctcgaga
     2581 cgctcgtgca gctcggtcgt ggccttgaag agcaggaaga ggccgccacc cagaaggatc
     2641 aggtccctga acgagaacgg gtgcccccag acggagaaga cggggtccgt gagggccacg
     2701 agccacgaca tgatggagag aaggcccaga cgcatcgcga gcgcaagtcc gaggcccacg
     2761 tagagggcct tgtcctgctg tgaggcggga agctttttcg cgatgatggc aataaagacg
     2821 agattgtcga tcccgaggac gatttcaaga acgacaagcg taagaagacc gacccagatg
     2881 gtcggatcaa gcagaaattc catgtgcaat tgagggatta agcacccgac ccgcggatgc
     2941 ccggagcgcg accgcaggct ttcctgatag agaagcgcac gatgacggac gcgtccgata
     3001 aaattaccat attttcatcg gtcgtcagtc ccccacagcg accttattca cggcccagca
     3061 acattctgaa aagatcttca tgccgcttac cgaacgcctg agcagccgcg tggctgccga
     3121 agcccttgcc ctggcaggtt ccgaaccgag gggcgtcccc gccgtcatcg acaccaacgt
     3181 cgtgctcgac ctcatctact gggaagaccc acattctgag gacttgagac gagccctcga
     3241 ggagggacgc atccatgcgc tgcgctcggg cgaggcggtc atggagcttg cggaagtgct
     3301 ctcgcgcccc gcgttcacgg gcgacgagga cgaggcggcg agactcgcag cgctctggtg
     3361 ctcgatgagc gtgcccgtcg acgaggagaa gatcagggag gcggccgacg tcatcacggt
     3421 ccagtgcagg gacccccttg accagaagtt cttcacgctc acgcttgcgg gcggcgcaaa
     3481 gcttcttgtg agcaaggaca agctcgtgct caaggccggt cgcaagatgc agcgatacgg
     3541 cgcctgcgcc ttccgtcccg aagagacggc ggcgaggctc gacgcgctca gctgagcgcg
     3601 tacttcgcca tggcaatgac gttgacgatg atccagaggg ccaccatggc gaaggcgacg
     3661 taaagcgcac cgcgcatgaa ctttcgcaac atgtacgact ccaaaaaaac agaggaagaa
     3721 aaaggccgat tattttagcc gtttccactc cgtctgagaa gcccccgttt cgtgtccccc
     3781 aatcccgtcg gaaccaccta gggaatacac cttagcctgt gccgcaggcc atccgccgag
     3841 tctccctaac cgacttgacg cgcatcaacg aaccccgaaa tcgtccgaaa cgatacgaaa
     3901 taccaccagc gccacacgca caggcgtttc ccccgaatct aggcagaatg cccatcccct
     3961 gaatgcgtcc cggggcatct cgcgattccc gaaccaattc atagaatgaa ggctcgggtt
     4021 ccgcctgaga acctgggacc gggcgaatca ttattcttca accgcttgtc cgcaggatcg
     4081 tttccaagtg tccgcatcct gcctccccgg tcagaatcat aaggcccaga ttcggacaaa
     4141 ctccgcccga agactgcccc acttttcaag caaaaaccgg agtcatcacc gtggatcaga
     4201 ccactcaaat cattcagagc atcaacagcc tgctctgggg catgtacggc ctcattccgc
     4261 tgctcgtcgg cacgggcctg tacttcacct tcaagcttcg attcgttcag atcaagcgct
     4321 tcgtcatggc cgccaggcac gtcttcggcg gcatcacgct cttcggccgc aaggccgaca
     4381 aggacggcat gtcctccttc cagtcgctcg cgacggccat ctcggcccag gtgggcacgg
     4441 gcaacctcgc cggcgtggcc accgccatcg ccatgggcgg tccgggcgcc atcttctgga
     4501 tgtggatcgc cgccttcttc ggcatggcga ccatcttctc cgaagctgtc cttgcccagc
     4561 tctaccgcac ccgcgacgct cacggccacc tgaccggcgg cccggcctac tacatcagcc
     4621 agggtctcgg cagcaagacg ctcgccg
//
LOCUS       DBMDTI010000148         4631 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4422_length_4631_cov_2.281898, whole genome shotgun sequence.
ACCESSION   DBMDTI010000148 DBMDTI010000000
VERSION     DBMDTI010000148.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4422_length_4631_cov_2.281898"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            313..1464
                     /locus_tag="ACZPB6_07290"
     CDS             313..1464
                     /locus_tag="ACZPB6_07290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1184306.1"
                     /translation="MTHLKPIAVLALILAATLEMRGPITAVGPLAGRLTSELGLSASE
                     YGLAAALPVAAFGLFSFAAPDAARRFGLYGAVIVFLGVLAAGGFVRTAPSYPALLLGT
                     LLIGVGIAALNVLMPVVVKTQNEAKTGALLGIYTGVVGLSGSIGALTSHPLAALTDFT
                     QSAFLAWAAFGAVVLALWLAAFGRSSGPSATLLRAPSLRRHPGAWAVTAVMGLQSLLI
                     YTVAAWLPPYLQTQGVSADAAGAALTLYLVSGLPASILTDRFMKHCGPEWASEALMSL
                     SYLAGLWFWTLGGPWIFLGSILAGAPQGSMLSVAFILMARKTRSSAHMLGLSALSQGV
                     GYLGAGLGPLVFAALLPAGWGASLSFVGFIIVLWALAGLAASRFSEIHP"
     gene            1573..2265
                     /locus_tag="ACZPB6_07295"
     CDS             1573..2265
                     /locus_tag="ACZPB6_07295"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR1184307.1"
                     /translation="MSPIEKLSSTPNPAVLLFDFESAPAGLEESLAATLPEATRSRIT
                     AFCHPVRRRQTRWGRILASAAARILDAELVEEPPYAPYLLKDGRRTALCIAHTGTSIA
                     LGIASVKDPVMGLDLETMRPVRSIEGMSRMSFGEAASAIVRQCAESGDSEPFFRAWGM
                     KESEIKLNRGGSGWRLTLDEESRPVVLDPEGRPVLATHAAFGSLRLTVLTGACAPVIG
                     RVTPADISRTLL"
     gene            2417..4150
                     /gene="tamA"
                     /locus_tag="ACZPB6_07300"
     CDS             2417..4150
                     /gene="tamA"
                     /locus_tag="ACZPB6_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0089705 - protein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HIR1184308.1"
                     /translation="MRRSLTAVLALSLCSGSALAADTLRIDIKGLDGDLAANARAWVE
                     PLEKAGYTDTPAARMNVDKAVRLSLRALGYYQPAVSMTVKDKRSLEITVVPGEPVLIS
                     DVDVHVTGSGADERVFKFLQKRRVPKKGDVLNHAKYEEFKSSLTNAALQLGYFDAEFV
                     KSQLGVSIPLREAVWSIDYETGERWRFGDVTFSGSQIDEELLRNMTPFKKGDYYNADV
                     LGDFNQKLASVGWFKSAVVAPDFQAARDAGTTELPMNCDVTPRAANIFEVGLGYATDT
                     GPRAKFGWKKPWINSRGHSLSSSTEVSSDEQLVDLSYKIPVVANPLEEYWLAQGAMKR
                     DVVNDTDSNNMTLALSRNWEFSTGWQRSIGVHWMFDSFTQAGVSSNTMLVYPSISFSR
                     TRSRGGMMPYWGDTQRYSIDTGTHYLGSDVDFIIMQAHGTLIRTPWRGHRFVVRGSAG
                     KIKTNDFDEVPPDLRFFAGGDRSVRGYKYESISPRDSEGRLTGASCMVTGSIEYQYNL
                     TGKWWTAVFFDVGEASNSFASFDWKRGAGVGIRWNSPVGPVKLDVAKPVAADAGEDDG
                     LQFYIGLGAEL"
     gene            4147..>4631
                     /locus_tag="ACZPB6_07305"
     CDS             4147..>4631
                     /locus_tag="ACZPB6_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184309.1"
                     /translation="MRRSAKVLLSAGGVLVLTAAGLAWLVATPSGQQMLLRTALPLVP
                     GLKAGSIEGGFFDLTITDLSYEMPGLTAGADRITLRLDRDALLSERRIAVTKLEILHP
                     HGVLDSASLPPSDPSAPSADTGPVTMPWPVSLDNAEITSLDFTADGTKVTLAYFAASA
                     S"
BASE COUNT          754 a         1751 c         1273 g          853 t
ORIGIN      
        1 gttgaattct gatttttaaa gaacagcgct cctaagagca cagaattgaa agcttaccaa
       61 ctcatcaact ctttgtcaaa tttattcgct gatgacttgg cgtcgtcagc gaaggattgg
      121 aattaaacac gactccggca cgtttgtcaa acatttgcgt caaaatccgt attactacct
      181 agaaaacctc ctcaggccat cattccccgt cacccgatct ttcgccgcgc atggacttcg
      241 accgcatttg gaggtatctt cttctggacc attccggagg cgcccgcggc ctcttcaaca
      301 accaccacct tcatgactca tctcaagccc attgcagtgc tcgccctgat tcttgccgcc
      361 acgctcgaga tgcgcggtcc gatcacggcc gtaggcccgc tcgcagggcg ccttacctcc
      421 gagctcggcc tctccgcctc cgaatacggc cttgcggccg cccttccggt tgcggccttc
      481 gggctcttct cgttcgccgc cccggacgca gcccgccgct tcggactcta cggcgccgtc
      541 atcgtctttc tcggcgtgct tgccgccggc ggattcgtgc gcacggcgcc gagctatccc
      601 gcgctccttc tcggcacgct tctcatcggc gtgggcatcg ccgccctcaa cgtcctcatg
      661 cccgtcgtgg tcaagacgca gaacgaggcg aagacaggtg cgctcctcgg gatctacacg
      721 ggcgtcgtcg gcctttcggg ctcgatcggc gcactcacct cgcatccgct cgcggccctc
      781 acggacttca cccagtcggc cttcctcgcg tgggccgcct tcggcgccgt cgtcctcgcc
      841 ctctggctcg cagccttcgg ccgctcgtcg ggcccctccg ccacgctcct tcgcgcccca
      901 tccctcaggc gccatcccgg cgcatgggcc gtcacggccg tcatgggact gcagtcgctc
      961 ctcatatata cagtcgccgc gtggctcccg ccctatctcc agacacaggg cgtcagcgcc
     1021 gacgccgcag gcgctgccct caccctctat ctcgtctcgg gcctccccgc ctccatcctc
     1081 accgatcgct tcatgaagca ctgcggtccc gaatgggcct cagaagccct catgtccctc
     1141 tcctatcttg caggcctctg gttctggacg cttggcggcc cctggatctt cctcggcagc
     1201 atccttgcgg gcgcccctca gggctccatg ctctccgtcg ccttcatcct gatggcaagg
     1261 aagacgcgct cctcggccca catgctcgga ctctcggcgc tcagccaagg cgtgggctac
     1321 ctcggcgcgg gcctcggtcc cctcgtcttc gccgcgctcc tccccgcagg ctggggcgcc
     1381 tcgctctcct tcgtcggatt catcatcgtc ctctgggccc tcgcaggcct tgcggcctcc
     1441 cgcttttcgg aaatccatcc ataaggatga tccgtccgcc ctaagcctgc gttttctgta
     1501 cactgtcagc gcccgggcga atcctcctga tccgtcccga atccattcat cttcaatacg
     1561 gtccgcctca tcatgtcccc aatcgagaag ctcagcagca ccccgaaccc cgccgtgctt
     1621 ctgtttgact tcgaatccgc tccagccgga ctcgaggagt cgctcgccgc gacgcttccc
     1681 gaggcgaccc gcagccgcat cacggccttc tgccaccccg tgcgccgccg ccagacccgc
     1741 tggggccgca ttctcgcaag cgccgccgcg cgcattctcg acgccgaact cgtcgaggag
     1801 ccgccctacg ctccctatct tctcaaggac ggacgccgca ccgcgctctg catcgcccac
     1861 acgggcacga gcattgcgct cggcatcgcc tccgtcaagg atcccgtcat gggactcgac
     1921 cttgagacga tgcgccccgt gcgcagcatc gagggcatgt cccgcatgag cttcggcgag
     1981 gccgcctccg ccatcgtgcg ccagtgcgcc gagagcggcg actcggaacc cttcttccgc
     2041 gcctggggca tgaaggagtc cgaaatcaag ctcaaccgcg gcggctcggg ctggcgcctc
     2101 acgctcgacg aagaaagccg tcccgtcgtc ctcgatcccg aggggcgccc cgtcctcgcc
     2161 acgcacgccg ccttcggcag cctgcgcctc accgtcctca caggcgcatg cgcgcccgtc
     2221 atcggacgcg tcacccccgc cgacatctcc cgcacgctgc tctgaaagcc tccgtcgccg
     2281 cctgcgcccc attcgatttc aggcaaatgg tttgaggttg caactgatgc cttgcgggcg
     2341 cgccacatgg gctacactca tgaattggcc gggccctgcg tccggcacgt gttcatcact
     2401 ccgaaggata tacgccgtgc gccgttcatt gaccgccgtt ctggcgcttt cactctgctc
     2461 cggctccgcg ctcgccgccg acacgctcag gatcgacatc aagggacttg acggtgatct
     2521 ggcggccaac gcccgcgcct gggtcgaacc actcgagaag gcaggctaca ccgatacgcc
     2581 cgccgcccgc atgaacgtcg acaaggccgt caggctctcc ctgcgcgcac tcggctacta
     2641 ccagcccgcg gtctccatga cggtcaagga caagcgtagt ctcgagatca cggtcgttcc
     2701 gggcgagccc gttctcattt ccgacgtcga cgtgcacgtc acgggctcgg gcgccgacga
     2761 gcgcgtcttc aagtttcttc agaagcgccg cgtccccaag aagggcgacg tgctcaacca
     2821 cgccaagtac gaggagttca agagctccct cacgaacgcg gccctgcagc tcggctactt
     2881 cgacgcagaa ttcgtcaaga gccagctcgg cgtctccatt cctcttcgcg aggccgtctg
     2941 gtccatcgac tacgagaccg gcgagcgctg gcgtttcggc gacgtgacct tctcgggctc
     3001 gcagatcgac gaggagcttc ttcgcaacat gacgcccttc aagaagggcg actactacaa
     3061 cgccgacgtg ctcggcgact tcaaccagaa gctcgcctcc gtgggatggt tcaagtccgc
     3121 agtcgtcgcg cccgacttcc aggccgcgcg cgatgcgggc acgaccgagc tgccgatgaa
     3181 ctgcgacgtc acgccccgcg cggccaatat cttcgaagtc ggtctcggct acgccaccga
     3241 cacgggcccg cgcgccaagt tcggctggaa gaagccatgg atcaacagcc gcggccacag
     3301 cctctcgtcc tcgaccgagg tctcctcgga cgagcaactc gtcgacctct cctacaagat
     3361 tcccgtcgtc gccaatccgc ttgaggaata ctggctcgcc caaggcgcca tgaagcgaga
     3421 cgtcgtcaac gacacggact ccaacaacat gacgctcgcg ctctcgcgca actgggagtt
     3481 ctccacgggc tggcaacgct cgatcggcgt ccactggatg ttcgactcgt tcacgcaggc
     3541 aggcgtctct agcaacacca tgctcgtcta tcccagcatc agcttctcgc gcacccgcag
     3601 caggggcggc atgatgccct actggggcga cacccagcgc tacagcatcg acacgggcac
     3661 ccattatctc ggcagcgacg tcgacttcat catcatgcag gcccacggca cgctcatccg
     3721 cacgccctgg cgcggacacc gcttcgtcgt gcgcggcagc gcgggcaaga tcaagacgaa
     3781 cgacttcgac gaagtcccgc ccgacctgcg attcttcgcg ggcggcgacc gctccgtgcg
     3841 cggctacaag tacgagtcca tctcgccgcg cgacagcgag ggacgcctca cgggcgcatc
     3901 ctgcatggtg acgggcagca tcgaatatca gtacaacctc acgggcaagt ggtggacggc
     3961 cgtcttcttc gacgtgggcg aagccagcaa cagcttcgcg agcttcgact ggaagcgtgg
     4021 cgccggcgtc ggcatccgct ggaactcccc cgtgggtccc gtcaagcttg acgtcgccaa
     4081 acccgtcgcc gcagacgccg gcgaggacga cggtctccag ttctacatcg gtctcggagc
     4141 tgaactatga gacgttcggc caaagttctt ctcagcgccg gcggcgttct cgtgctgacg
     4201 gccgcgggcc tcgcatggct cgtcgccacg ccgtcgggcc agcagatgct gcttcgcacg
     4261 gcccttcccc tcgtgcccgg cctcaaggcg ggctcgatcg agggcggctt cttcgacctc
     4321 acgatcacag acctctccta cgagatgccg ggcctcactg caggcgccga ccgcatcacg
     4381 ctgaggctcg accgggacgc gctcctctcc gagcgccgca tcgccgttac gaagctcgag
     4441 atcctgcacc cgcacggcgt actcgactcc gcatcgctgc cgccctccga cccgtccgcc
     4501 ccctccgccg atacgggccc cgtcaccatg ccgtggcccg tatcgctcga caacgccgaa
     4561 atcacgagcc tcgacttcac cgccgacggc acgaaggtga cgctagccta cttcgccgcc
     4621 tccgcctcct g
//
LOCUS       DBMDTI010000149         4612 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4450_length_4612_cov_2.356905, whole genome shotgun sequence.
ACCESSION   DBMDTI010000149 DBMDTI010000000
VERSION     DBMDTI010000149.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4450_length_4612_cov_2.356905"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(44..616)
                     /locus_tag="ACZPB6_07310"
     CDS             complement(44..616)
                     /locus_tag="ACZPB6_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184310.1"
                     /translation="MGSVLNQADDVSLAVRRLVFGEMKVCGITRADDATRAALEDFSA
                     AGVVLAKRSPRAVTPEGLAVLSNEIKERTKALGLTMPVTAVVDTDETDVLRAIASPAA
                     TDAFTPLQIHGDVTDADLEGLRVLFPGKALHLAAALPEDPVALAEAARRLEGLLERGL
                     ADRIVVDGTSDGLAGAVRGCPRRRPENRTH"
     gene            complement(571..1044)
                     /locus_tag="ACZPB6_07315"
     CDS             complement(571..1044)
                     /locus_tag="ACZPB6_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014884.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184311.1"
                     /translation="MDARLETLYRMQPLSAAETDALFTEVFAGSMSEPELASLLTALK
                     MKGETPDEIRGAAAAMLRAARPFPKSAFEVGEIVGTGGDGAGTINVSTMTALAAAGAG
                     LRIAKHGNRGVSSPSGASSPSPASAPTPTSSASHARPASSSWAPSSIRPTTSRSP"
     gene            1839..3614
                     /locus_tag="ACZPB6_07320"
     CDS             1839..3614
                     /locus_tag="ACZPB6_07320"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HIR1184312.1"
                     /translation="MAVQIDQSRDALFDELGLMRLRESYMRADEKSPQERFKFVAETF
                     GSNPEHAARIYEYASRHWLSFATPILAFGRTGRGLPVSCYLSYMDDSAEGLIETLSEV
                     NRLSMLGGGVGIHVGIRSADEKSVGVMPHLKVYDASCLAYRQGSTRRGSYAAFLSIDH
                     PDIIPFIEMRKPTGDQNLKALNLHHGVNITDAFMEKIEASMRDADADDSWDLRHPTTG
                     EVVATVSARDLWQRLLEMRMHTGEPYLVFTDTANRALPSWLKDKGLSIHGSNLCTEIF
                     LPTSKDRTAVCCLSSVNLEYYDEWRGNEQFIDDAMEFLDNVLQYFIEHAPDGIRRARY
                     SAMRERSVGLGAMGFHAYLQKRGIAFESAAAKFTNRDMFRHIAAAVEKADARLCAERG
                     PCPDAADSGVMRRFSHRMAVAPNASSSLIMGGTSPSIEPYRANIYRQDTISGAYIVKN
                     RFLKAELAKRGLDTDDVWADIIAHDGSVQHREDLPKEVRDVFKTAIELDQRWIIELAA
                     DRQAFIDQGQSVNLFFPPDVSIAYLHACHFSAWKKGLKSLYYNRSDKLRKADRVGVQI
                     ERRRIEDQISMQAVADNTTCLACEG"
     gene            3632..>4612
                     /locus_tag="ACZPB6_07325"
     CDS             3632..>4612
                     /locus_tag="ACZPB6_07325"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIR1184313.1"
                     /translation="MTDKNTAGGLLSTRTCFKPFKYPWAYEAFMQSEQMHWLWTEVPM
                     LEDVKDYGQKLTDSERDFLTKILRFFTQGDIDVSGAYVNHYLPRFGQPEVRMMLSSFA
                     AREAVHIAAYSHLIETIGLPESIYNEFLQYEAMAAKHDFFNEVAEDSRDLAAQIAAFS
                     AFTEGMQLFSSFVMLLNFTRNGTMKGMGQIIAWSIADETLHTESMTKLFREYIVENPE
                     LWNDSLKAKIYGIAETMVELEDRFIDLAFGVSEMRRLTREEVRSYIRYIADRRLIGLG
                     MKGIFGIKKNPLPWVDAMLGVSHTNFFENKVVDYAKGALTGDWGDVWGVAN"
BASE COUNT          822 a         1550 c         1368 g          872 t
ORIGIN      
        1 cagcatgatg gagattcctt tttcgagttc ggttctgtgt agttcagtgt gttcggtttt
       61 cgggtctgcg tcgggggcat ccccgcacgg cgcccgcgag gccgtccgag gtgccgtcga
      121 cgacgatcct gtcggcgagt ccccgctcga gaagcccctc gagcctgcgg gccgcctcgg
      181 cgagcgcgac ggggtcttcg gggagagcgg ccgcgagatg gagggccttt cccggaaaca
      241 ggacacgaag tccttcgagg tctgcgtccg tcacgtcacc gtggatctgt aggggcgtga
      301 aggcgtctgt ggcggcgggc gaggcgatgg cccggagaac gtccgtttcg tctgtgtcga
      361 cgacggccgt gacgggcatc gtgaggccga gggccttcgt gcgttccttg atctcattcg
      421 agaggaccgc gagtccttcg ggtgtgaccg ctctgggtga gcgcttcgcg agcacgacgc
      481 ctgctgcgga aaagtcctcg agcgcggccc tggtcgcatc gtcggcccgt gtgatgccgc
      541 agaccttcat ttcgccgaag acgagtcttc tcacggcgag cgagacgtcg tcggcctgat
      601 tgaggacgga gcccacgagg aagacgcggg ccgcgcgtgc gacgcgctcg acgtcggcgt
      661 gggtgcggat gccggactgg gcgacgaggc gcccgacggg gacgacacgc cgcggttgcc
      721 gtgcttggcg atcctgagac ctgcgcccgc cgcggcgagc gccgtcatcg tcgagacgtt
      781 gatcgtgccc gcgccgtcgc cgcccgtgcc gacgatctcg cccacctcga aggcggattt
      841 cgggaagggc ctcgcggcgc gcagcatcgc ggcggccgct ccgcggattt cgtcgggcgt
      901 ctcgcccttc atcttcaggg cggtgagaag ggacgcaagc tcaggctcgc tcatgctgcc
      961 tgcaaagacc tcggtgaaga gggcgtcggt ttcggcagcg ctcaggggct gcatgcggta
     1021 gagggtttca agtcgggcat ccatttcgtg ctcctttcat tcccggtcgg ctttttccgt
     1081 tggttttcaa ttgattttca gttgatcctg tgttttcgcg gacgcttcag gggaggcgtc
     1141 ccgcggggat tccggccgtg ccggcgggcg cgcggagaaa gccgggccag ggcctggcct
     1201 cgggcttgag ggtcttcagg agcggttgtc tgatgagggg gagaactggg tttgacatcg
     1261 caaaaccttt gtctggtgct tgcggttgcc gaaccggagg gagagactgg ataaaagaaa
     1321 acccgcagat tcgccatccg cgggttgtgg ggaaactgtt gacttgcgtt cttgatgctc
     1381 gtgactatct ctgggcggta cccgcttgct gggggtgcca ccaccaaaga agagactgag
     1441 cgttgaaggc gtgcgtcatg atgacttccg tttccaaatc cgattgatct tttgattgat
     1501 cttgaactgc aggtgccgtt cgaggcaccg cttccgcata ctctacgccc ccataccgca
     1561 ttcgcgcaat aggtcaaaca ccctggttcc tctgaaaagc gtgcaaagtc ccgtcgcaca
     1621 agcctcgtaa ataatcctta ttgtccggtt aggcctccct tattggcgcg cgggcgggtt
     1681 tctctgtact atgagactca cttcacggac gcaagttctt gtgtccgttc acgcttcaaa
     1741 ccacaagatg ttgtgggtgt gccttcaggc gcctctgccg cagacatctg ccaggattcc
     1801 acgaaattcc gaaatccctt ttgaaaggaa ccccatcgat ggccgttcag atcgatcaga
     1861 gccgcgacgc gctctttgat gagctcgggc tcatgcgcct tcgcgaaagc tacatgcgcg
     1921 cggacgagaa gagtccgcag gagcgcttca agttcgtggc cgagaccttc ggctcgaacc
     1981 cggagcacgc cgcccgcatc tacgaatacg caagccgcca ctggctttcc tttgcgacgc
     2041 ccattctcgc cttcggccgc acgggccgcg ggcttcccgt ctcgtgctat ctctcctaca
     2101 tggacgacag cgccgaaggc ctcatcgaga cgctctccga ggtcaaccgc ctctccatgc
     2161 tcggcggcgg cgtgggcatc cacgtcggca tccgctcggc cgacgagaag tccgtgggcg
     2221 tgatgccgca cctcaaggtc tacgacgcct cctgcctcgc ctaccgtcag ggttctaccc
     2281 gccgcggctc ctacgccgcc ttcctctcga tcgaccatcc ggacatcatt ccgttcatcg
     2341 agatgcgcaa gcccacgggc gaccagaacc tcaaggccct caatctgcac cacggcgtca
     2401 acatcaccga cgccttcatg gagaagatcg aggcctcgat gcgcgacgcc gatgcggacg
     2461 actcctggga tcttcgtcac ccgaccacgg gcgaggtcgt cgcgaccgtc tccgcccgcg
     2521 acctctggca gcgcctcctt gagatgcgca tgcacacggg cgagccctat ctcgtcttca
     2581 ccgacacggc caaccgcgcg ctgccctcct ggctcaagga caaggggctc tccattcacg
     2641 gctccaacct ctgcaccgag attttcctgc cgacctcgaa ggaccgcacg gccgtctgct
     2701 gcctctcctc cgtcaacctc gagtactacg acgagtggcg cggcaacgag cagttcatcg
     2761 acgacgcgat ggagttcctc gacaacgtgc tccagtactt catcgagcac gcgcccgacg
     2821 gcatccgccg cgcgcgctac tccgccatgc gcgagcgtag cgtgggcctc ggcgccatgg
     2881 gctttcacgc ctatctgcag aagcgcggca tcgccttcga gtccgccgcc gccaagttca
     2941 cgaaccgcga catgttcagg cacatcgccg cagcggtcga aaaggccgac gcgcgcctgt
     3001 gcgccgagcg cggcccctgt cccgacgcgg ccgactccgg cgtcatgcgc cgcttctcgc
     3061 accgcatggc cgtcgcgccc aatgcctcct cgtccctcat catgggcggc acgagtccct
     3121 cgatcgagcc ctatcgtgcc aacatctacc gtcaggatac gatctccggc gcctacatcg
     3181 tgaagaaccg cttcctcaag gccgagctcg ccaagcgcgg cctcgacacg gatgacgtct
     3241 gggccgacat catcgcccac gacggctccg tgcagcaccg cgaggacctt ccgaaggaag
     3301 tgcgcgacgt cttcaagacc gccatcgaac tcgaccaacg ctggatcatc gagcttgccg
     3361 ccgacaggca ggccttcatc gaccagggcc agagcgtgaa cctcttcttc ccgcccgacg
     3421 tttcgatcgc ctatctgcac gcctgccact tctccgcctg gaagaagggc ctcaagagcc
     3481 tctactacaa ccgctccgac aagctccgca aggcggaccg agtcggcgtc cagatcgagc
     3541 gccgccgcat cgaggatcag atcagcatgc aggccgtcgc cgacaacacc acctgcctcg
     3601 cctgcgaagg ctgaccggag ggaaccacat catgaccgac aagaacaccg ccggcgggct
     3661 cctttcgacc cgcacctgct tcaagccctt caagtacccc tgggcctacg aggccttcat
     3721 gcagtccgag cagatgcact ggctctggac cgaagtgccc atgctcgagg acgtcaagga
     3781 ctacggccag aagctcaccg acagcgagcg cgacttcctc acgaagatcc tgcgcttctt
     3841 cacccagggc gacatcgacg tctcgggcgc ctacgtcaac cactacctcc cgcgcttcgg
     3901 tcagcccgag gtccgaatga tgctctcgtc gttcgccgcg cgcgaggccg tgcacatcgc
     3961 cgcctactcg cacctcatcg agacgatcgg gctccccgag tccatctaca acgagttcct
     4021 gcagtacgag gccatggccg ccaagcacga cttcttcaac gaagtcgccg aggacagccg
     4081 cgacctcgcc gcgcagatcg ccgccttctc ggcattcacc gagggcatgc agctcttttc
     4141 gtccttcgtc atgctgctca acttcacgcg aaacggcacg atgaagggca tgggccagat
     4201 catcgcctgg tccatcgccg acgagacgct ccacaccgag tccatgacga agctcttccg
     4261 cgagtacatc gtcgagaacc ccgaactctg gaacgactct ctcaaggcca agatctacgg
     4321 catcgccgaa accatggtcg aactcgagga ccgcttcatc gacctcgcct tcggcgtctc
     4381 cgaaatgcgc cgcctcaccc gcgaggaggt ccgcagctac atccgctaca tcgccgaccg
     4441 ccgtctcatc ggtctcggca tgaagggcat ctttggcatc aaaaagaatc cgcttccgtg
     4501 ggtggatgct atgctgggtg tatcacacac gaactttttc gaaaataaag tcgtggatta
     4561 tgcaaagggc gcccttacag gtgactgggg cgacgtctgg ggcgttgcaa at
//
LOCUS       DBMDTI010000150         4551 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4520_length_4551_cov_3.237978, whole genome shotgun sequence.
ACCESSION   DBMDTI010000150 DBMDTI010000000
VERSION     DBMDTI010000150.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4520_length_4551_cov_3.237978"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(156..515)
                     /gene="ssrA"
                     /locus_tag="ACZPB6_07330"
     tmRNA           complement(156..515)
                     /gene="ssrA"
                     /locus_tag="ACZPB6_07330"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(533..1204)
                     /locus_tag="ACZPB6_07335"
     CDS             complement(533..1204)
                     /locus_tag="ACZPB6_07335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1184314.1"
                     /translation="MTIRLFLFDLDGTLVDTAPDLALAVNMVRTARGIAPLPEEELRP
                     YASRGAPGLLGKAMGIGRDDPAFLALRTEFLDNYAAHMTDRSRPFEGVPVMLEALRAA
                     GIRTGVVTNKVEHLALRLTDNLGLDGQLDVVLGSDSEGCAMKPAPDSLLTAARLTGVH
                     VTDILYAGDDPRDVAAAHAAGMPCAACTWGYAVTPPESWGADLVAHSPAELATWAVQL
                     NAHAL"
     gene            complement(1311..1970)
                     /locus_tag="ACZPB6_07340"
     CDS             complement(1311..1970)
                     /locus_tag="ACZPB6_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR1184315.1"
                     /translation="MKTSLKIGGLVAAMMLAVSGASFAADQVSPYVHSADGQIVKSGF
                     GLCWRTNFWTPALAEASGEGCQCDADVLPAEACQPKQAAAPVAPAAEKVTFSADMLFN
                     FDKSTLKDEGKAVLDDLVSRLAGVNVEVILSTGYADRLGKDAYNEKLSAARAESVKTY
                     LVSKGVDANLIQTEGRGEADPVVDCPNPSAKGQVKNFRELVQCLAPNRRAVVEVVGTR
                     Q"
     gene            complement(2272..3576)
                     /locus_tag="ACZPB6_07345"
     CDS             complement(2272..3576)
                     /locus_tag="ACZPB6_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HIR1184316.1"
                     /translation="MTADTLVEARWVIPVIPRNTVLENHTVVFSGGRIADILPTEAAR
                     VRYAGLEAKTMRLPAGVVMPGLINLHTHAAMNLLRGRGADLPLMDWLTKEIWPAEGRL
                     MSAEFVHDGSLIAGVEMIRSGVTCCSDLYFFPEAAAEGLREAGLRCATAGIVIGFPSA
                     WAANDDEYISKCEALIERYSGDPFVRVTVGPHAPYTVSDASLARCAALSERHDLPVHI
                     HVNETAGEVETSLKDHGERPVARLARLGLLNERLISVHSVHTSNEDIRMLAEARASVC
                     HCPSSNLKLASGFAPVAKMMKAGINLGIGTDGAASNDKLDMLGETRLAAMLAKAVAGA
                     TTCAKVFDMLEAATLGGARALHWDDEIGSLEIGKCADMIAVDLSGIECGPVTDPAAQL
                     LYSAGREHVTHVWVAGRLAAGEGTEPRDDTARTLLEKWAPLI"
     gene            4019..>4551
                     /locus_tag="ACZPB6_07350"
     CDS             4019..>4551
                     /locus_tag="ACZPB6_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR1184317.1"
                     /translation="MKRSYLDYAMSVIVGRALPDVRDGLKPVHRRVLHAMNEIKNTHN
                     NPTKKAARVVGEVLGKYHPHGDSAAYMTIVRMAQPFSLRYPLIFGQGNFGSIDGDSPA
                     AMRYTEVRLEKIAGEMLADINEDTVDFVPNFDNSEREPVVLPTRLPQLLINGSSGIAV
                     GMATNIPPHNLGETIDA"
BASE COUNT          867 a         1331 c         1534 g          819 t
ORIGIN      
        1 ttttgcgata tttcgacatt ttgtttccgc ggggcgccgt gcacgacgta gggaagcgaa
       61 agggcggggt gtgatgcgca ggaagggacg gcggagcggg gtgcctgaaa gcagaagggc
      121 ctgagatcgt gcagaacaat ctcaggccct gaatatggtg gaggcggcgg gagttgaacc
      181 cgcgtccgga aacctttttc tgttgggtct acatgcttag ccgcatgatt tgaatctcac
      241 cggatgccct gccgagcggc gggccggcat ccagccagtc accttgattt cggaagcgcg
      301 tcggtgacgc ccgcacatcc tagactctgt caaatgatac tgctgtcgat tgctcgaccc
      361 ggcccagaga caaaccggtg cagcatctcg cattaagcag cgagagcgaa acgcttgttg
      421 ttggcgttta attgtttgca gcggatttac gaggtgactg cacctcgaca tgccccatgc
      481 agcgtccgcg tccccgtcga agccaaggcg ccccctgtat gaaggatgac cattataacg
      541 catgagcgtt gagctgcacg gcccatgtcg cgagttcggc gggcgaatgt gcaacaagat
      601 ccgcacccca gctttcgggc ggcgtgactg catagcccca ggtgcaggcc gcgcagggca
      661 tgcccgccgc atgcgccgct gccacgtctc tcgggtcgtc gcctgcgtag aggatgtccg
      721 tgacgtgaac gcccgtgagt cgggcggccg tcagcaggga gtcgggcgca ggcttcatcg
      781 cgcagccttc ggagtcgctg ccgagcacga cgtcaagctg tccgtcgagt ccgaggttgt
      841 ccgtgagcct gagtgcaagg tgctcgacct tattggtgac gacgcccgtc cggatgcctg
      901 cggcccggag agcctcgagc atgacgggga cgccctcgaa gggacgggag cggtccgtca
      961 tgtgggcggc gtagttgtcg aggaattcgg tcctgagggc gaggaaggcg ggatcgtccc
     1021 tgccgatgcc catggccttg ccgagaaggc cgggggcgcc cctgctcgcg tagggacgga
     1081 gctcctcttc gggaagcggg gcgatgcccc tggcggtcct caccatgttg acggcgagcg
     1141 caaggtcggg tgcggtgtcg acgagggtgc cgtcaaggtc gaagagaaaa agcctgatgg
     1201 tcatggtcgt gctgtcctga gaggaggaat aaagaaagcc ctcgaacgtc tggcattcga
     1261 gggcttgccg agggcgcttg agcgcgccct cacggaactt cagtcaggaa ttactgacgc
     1321 gtaccgacga cttcaaccac ggcgcggcgg ttcggagcga ggcactgaac gagttcgcgg
     1381 aagttcttga cctggccctt ggcggagggg ttcgggcagt caacaaccgg atcggcctca
     1441 ccgcggcctt cggtctggat caggttggcg tccacgccct tggagacgag gtaggtcttg
     1501 acggattcag cacgagcagc ggagagcttt tcgttgtagg cgtccttgcc gagacggtcg
     1561 gcatagccgg tggagaggat gacttcaacg ttcacgccgg cgagacggga gacgaggtcg
     1621 tcgagaacgg ccttgccttc atccttgagg gtggacttgt cgaagttgaa gagcatgtcg
     1681 gcggagaagg tcaccttttc ggcagcggga gcaaccggag cggcggcctg cttcggctgg
     1741 caggcttcgg cagggagaac atcggcgtcg cactggcaac cttcaccgga ggcttcagcg
     1801 agagcgggcg tccagaagtt ggtgcgccag cagagaccaa agccgctctt gacgatctga
     1861 ccgtcagcgg agtggacata agggctgacc tggtcggcag cgaaggaagc gcccgacacc
     1921 gccagcatca tagcggccac aaggccgcca atcttcagag aagtcttcat tgttgttatc
     1981 ctcaaaaagt tcaggaagct taaacgggat ggacgatttg tcaccaacag gattgccggc
     2041 tacaaacaga tcacccagat gtcttgcccg ttaactcgga caggatacac cgaacacgtc
     2101 aagcgtgcga aggtcagtgc tgtcctgcgg ggtcaaatgg ctaaatatct aggttcattc
     2161 tgtcagattt ccgcaacgat ttccaccgct ggacatcccg tattacacct aaagccgatt
     2221 tatcggggag ggaaaaaaac cgcctggtcg ccgctgtgac cgcgcgggga ctcaaatgag
     2281 gggcgcccac ttttcgagca gcgtgcgagc agtgtcgtct ctcggctccg tgccttcgcc
     2341 tgcggcaagc ctgcctgcaa cccagacgtg ggtcacgtgt tcacggcccg ccgaataaag
     2401 aagctgggcg gctgggtcgg tgacgggacc gcattcgatg cccgagaggt cgaccgcgat
     2461 catgtcggcg cattttccga tttccagaga accgatttca tcgtcccagt gaagggcgcg
     2521 cgcgccgccg agcgtcgcgg cctcgagcat gtcgaagacc ttcgcgcagg tggtggcgcc
     2581 cgcgacggcc ttggcgagca tggccgcgag gcgggtctcg ccgagcatgt cgagcttgtc
     2641 gttgcttgcc gctccgtcgg tgccgatgcc gaggttgatg cccgccttca tcatcttcgc
     2701 gacgggcgcg aagcccgagg cgagtttgag gttggaggac ggacagtggc agacggatgc
     2761 gcgtgcctcg gcgagcatgc ggatgtcctc gttgctcgtg tgcacgctgt gcacggagat
     2821 gaggcgctcg ttgagaagtc cgaggcgggc aaggcgggcg acggggcgct cgccgtggtc
     2881 cttgagggag gtctccacct cgcctgcggt ttcattgacg tggatgtgca cgggcaggtc
     2941 gtggcgctcg ctcagggcgg cgcatcgcgc aagggaggcg tcgctcaccg tatagggcgc
     3001 gtgcggcccg acggtcacgc gcacgaacgg gtcgccgctg taacgctcga tgagcgcctc
     3061 gcacttgctg atgtactcgt cgtcgttcgc ggcccaggcg gacgggaagc cgatgacgat
     3121 gcctgcggtc gcgcagcgca gccccgcctc gcgaaggcct tcagcggcgg cttcggggaa
     3181 gaagtagagg tccgaacagc aggtgacgcc cgagcggatc atttcgacgc ccgcgatcag
     3241 gctgccgtcg tgcacgaatt ccgcactcat gaggcgtccc tcggcgggcc agatctcctt
     3301 ggtgagccag tccatgaggg gaaggtcggc gcccctgccg cgaagcaggt tcatggccgc
     3361 gtgcgtgtga aggttgatga gaccgggcat gacgacgcct gcgggcaggc gcatcgtctt
     3421 cgcctcgaga cccgcgtagc ggactctggc ggcctcggtc ggaagaatgt ctgcgatgcg
     3481 gccgcccgag aagacgacgg tgtgattctc cagaacggtg tttcggggaa tgacgggaat
     3541 gacccagcgg gcctcgacga gcgtgtcggc ggtcatgcgc aacctcatga tttttgactc
     3601 gaaaacggcg cgggccgaca gggtttcgga cggtgtcggg aacctgtcgc cgtgcgggac
     3661 ttcagccaat tgtattcggg tttgaatcgg cgaataactg gtaaaataga ttgtttggac
     3721 cgacgcggtc acgcacgaga gggcgggcag cgaagcggtc gtcgcggcgg gtctccggac
     3781 cctgcgcgca tcagtcagac agacttaagg aaagaagagc cggaaggctt cgtccgggat
     3841 cagggacggg gcgccggcct tacccagagg aataaacggc atggacgaaa aagacaataa
     3901 ggaagagccg caggtgcagg gcccggaagc cgccgaggat gcggaagacg acgccccgct
     3961 tcagggatcc gccctgagcg tcgcgcacga gctccttccg atttcgcttg aatcggaaat
     4021 gaagcgctct tatctcgact acgccatgag cgtgatcgtg ggccgcgcgc ttcccgatgt
     4081 gcgcgacggc ttgaagccgg tgcaccgccg cgttcttcat gccatgaacg agatcaaaaa
     4141 cacccacaac aacccgacca agaaggccgc gcgcgttgtg ggtgaagtgc tcggcaagta
     4201 ccacccgcac ggcgactccg ccgcctacat gacgatcgtg cgcatggccc agcccttctc
     4261 gctgcgctat ccgctcatct tcggtcaggg caacttcggc tcgatcgacg gcgacagccc
     4321 cgccgccatg cgttacacgg aagtgcgcct tgagaagatc gccggtgaaa tgctcgccga
     4381 catcaacgag gacacggtcg acttcgttcc gaacttcgac aactccgaaa gggagcccgt
     4441 cgtcctgccg acccgtctgc ctcagcttct catcaacggc tcctccggca tcgccgtggg
     4501 catggccacg aacatcccgc cgcacaatct cggcgagacg atcgacgcct g
//
LOCUS       DBMDTI010000151         4459 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4616_length_4459_cov_2.942175, whole genome shotgun sequence.
ACCESSION   DBMDTI010000151 DBMDTI010000000
VERSION     DBMDTI010000151.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4616_length_4459_cov_2.942175"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="ACZPB6_07355"
     CDS             <1..206
                     /locus_tag="ACZPB6_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1184318.1"
                     /translation="QAERMIAAGKSVSKARILFSPAVRFFKYYFLKLGFLDGFPGFVH
                     ISIGCFTVFLKYVKACFGARAEK"
     gene            206..973
                     /locus_tag="ACZPB6_07360"
     CDS             206..973
                     /locus_tag="ACZPB6_07360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013882.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901271 - lipooligosaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HIR1184319.1"
                     /translation="MHDKMLVTYVINLKRSTERMENMDRALKAMQVGYERVEGVDAQL
                     LSEVLLSRNATPSVEYPYELTKGELACFYSHRKCWEMLVNSQHEWALVLEDHCEFSEA
                     AGRYLRSADWLPSECELVQLIYSDKACFADEKKPLDDGNSLVKLACSSAIGSSAYLIS
                     RGAAVEALRMSARISEPADNFLFGPWSEFSRKVPCWRLLGGVVRRRHGIKTTIAGRNV
                     RRKFYLTKRLSLKRLARKMQMALQKRHLQETVQYWLE"
     gene            complement(1004..1894)
                     /locus_tag="ACZPB6_07365"
     CDS             complement(1004..1894)
                     /locus_tag="ACZPB6_07365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR1184320.1"
                     /translation="MTLPALRLLEASGFTPMLTGKRWAEDLMSGMGWRFDPIEGHVTE
                     DLHRIRYISNHLGPRPLGLLFPNSFSSALLFSIGRVRNAGFPTDGRRFLLDKVVEEPG
                     TMHEVERFFRLAHGAIKAWGGTPAWDSVPEELGMRLLARHEAAARNLMAEHGIPENFA
                     LLAPIARGLHHGKEKQWKHFNELCGPLRDMGIEPIVFPSVREEEANHAACPNARILPP
                     TTLGNYAALAKRARIVIANDSGISHVAAAISANQITLLGVTDPARTRPWNSKAVCLGN
                     STDGWPSLEQVLAALKDSLR"
     gene            2134..3513
                     /locus_tag="ACZPB6_07370"
     CDS             2134..3513
                     /locus_tag="ACZPB6_07370"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HIR1184321.1"
                     /translation="MAVSVFDMFKVGIGPSSSHTVGPMRAGHAFVKVLRDRGMLEQAT
                     SLKIELMGSLAATGKGHGTDTAAQLGLLGRIPETMDPDEVPVLIGDIRASRQLKLDGV
                     HAVAFDPEVDIAFHADKVPVFHTNAMEFSVFAGDSLLYRRRYYSVGGGFIVAAREDDP
                     EQPVTPKAFQGVKTKPYPYRTGDELMKVARDNNLTIAELVYQNECVDRTPEEVDRRLD
                     EVWQVMHAAVERGMRQTEVLPGPFRIARRANALMQDVRQRTDDPLAVLDWVNVYAMAV
                     AEENAAGGRVVTAPTNGAAGVIPAVIEYYLRHVSGSTMEGVRTFLLTAGAVGMLYKEN
                     ASISGAEVGCQGEVGVACSMAAAGLAAVCGGTVEQIENAAEIGMEHNLGLTCDPVAGQ
                     VQIPCIERNAIAAVKAINAARMALRGNGSHYISLDQVIRTMMATGRDMQSKYKETSLG
                     GLAVSIVEC"
     gene            3549..>4459
                     /locus_tag="ACZPB6_07375"
     CDS             3549..>4459
                     /locus_tag="ACZPB6_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1184322.1"
                     /translation="MLIGVFFGIAACMIWGCVYVAPLILPDYSPVHIAMMRYLVFGML
                     AAGLAVPEWRELKSYSAADWRRALALGIVGNIFYYWLLAEACQRAGAPIAGAFTALIP
                     IMVGVIGNMRAGESAVPWRRIVPPLCLSFAGMVCLNWTEFIYLVGDGRVTSGNFWLGV
                     GFAFFSSLVWTWYPLSNAEWLLSHPGKSPKTWSTAQGLALLPCAAVGMALLAGDGTQG
                     DILGPRPWMFIATVVFLGVCASWIGIVLWSLMSQRLTPALGGQMIIFETIFAVTYAHL
                     WRSEWPTPLMLVGLGLLLMGVTASLKV"
BASE COUNT          860 a         1185 c         1448 g          966 t
ORIGIN      
        1 ttcaggctga gaggatgatt gcagcaggga aatctgtatc caaagccaga attctgttca
       61 gtccagcagt caggtttttc aagtactact ttcttaaact gggattcttg gacggattcc
      121 ctggttttgt acacatttcg ataggatgct tcaccgtgtt tctgaagtat gtgaaggctt
      181 gctttggtgc gagggccgaa aagtgatgca cgacaaaatg cttgttactt acgtcatcaa
      241 tctcaagcgc tctacagagc gaatggaaaa catggacagg gctctcaagg caatgcaagt
      301 cggctacgag cgtgtcgagg gagtggatgc gcagctgttg tccgaggttt tgctgtccag
      361 aaatgccacg ccgagcgtcg agtatccgta cgaactgacg aagggcgagc ttgcctgttt
      421 ctacagtcat cgcaagtgtt gggagatgct tgtaaattct cagcatgaat gggccttggt
      481 tctggaggat cactgcgagt tttccgaagc ggcaggccgc tacctgaggt ctgcagactg
      541 gttgccgtct gagtgcgagc ttgttcagct gatctattcg gacaaggcgt gcttcgcaga
      601 cgagaagaag cctctggatg acgggaacag tctggtaaag cttgcctgtt cctccgccat
      661 tggatcgtct gcatacttga tctctagagg tgcggccgtg gaagccttgc ggatgtcggc
      721 gaggatttct gaacctgcag acaacttttt gtttgggccg tggtcagagt tttcccgaaa
      781 ggtgccatgt tggagactgc tgggaggtgt agtgcgcaga aggcacggca tcaagacgac
      841 cattgcaggc agaaacgtca ggcggaagtt ctatctgaca aagagactga gtttgaagcg
      901 cttggcaaga aaaatgcaga tggcattgca aaaacgccat ctgcaggaaa cggttcagta
      961 ttggctggag tgaccagcct cgaaggcgtc acaggctgtt gaatcagcga agactgtcct
     1021 tcagtgcggc aagaacctgt tccagagagg gccagccgtc cgtgctgttg ccgagacaga
     1081 cggccttgct gttccagggg cgggttctgg cgggatcggt gacgccgaga agcgtgatct
     1141 gattggcgct gatggcggca gcgacatggc tgatgcccga gtcattggca atgacgatgc
     1201 gggcgcgctt ggccagagcc gcataattgc ccagcgtggt gggcggaaga attctggcgt
     1261 tcggacaggc cgcatggttg gcttcctctt cacgcacgga agggaagacg atgggttcga
     1321 tccccatgtc gcgaagcggt ccgcagagtt cgttgaagtg cttccactgc ttttccttgc
     1381 cgtgatgaag gccgcgggca atcggggcga gcagtgcgaa gttttcggga atgccgtgtt
     1441 cggccatcag gttgcgtgcg gcggcctcgt ggcgggcaag cagtctcatg ccgagttctt
     1501 caggaacgga atcccaggca ggggtgccgc cccaggcctt gatcgctccg tgggcaagac
     1561 ggaaaaagcg ctccacttca tgcatggtgc cgggctcttc aacgactttg tccaacagaa
     1621 agcggcggcc atccgtagga aagcccgcgt tgcgcacgcg cccgatggag aaaagcaggg
     1681 ccgaactgaa ggaattcgga aaaagcagcc ccagaggacg cggaccaaga tgattgctga
     1741 tgtagcggat tcggtgcaga tcctcggtga cgtgcccctc aatggggtcg aacctccagc
     1801 ccatgccgga catcaggtcc tccgcccaac gcttgcccgt cagcatgggt gtgaagccac
     1861 tggcctccag aaggcgaagg gcggggagag tcatgcagca gtcaccaaca tgattgggca
     1921 gacggcagag aacgttaggc atttgcgtaa aacccgaatt gagaaaatac aatgagcgta
     1981 gttattgtat atggtcagcc tgtatgatga gatctttgcg cggcgttctc ccgaagcagg
     2041 agtgcggacg gttgaggatg ctgcgggcgt cagtccgaag tctttcctgc ggagaggctt
     2101 tgtccctttt cggaatttga gtttcagtta aaaatggcag tcagtgtttt cgacatgttc
     2161 aaggtgggca tcggcccctc gtcttcgcat acggtgggac cgatgcgtgc ggggcatgcc
     2221 tttgtcaagg tgctcaggga tcgcgggatg cttgagcagg caacgtcgct caagatcgag
     2281 ctgatgggaa gtctcgccgc gacaggcaag gggcacggca cggacacggc ggcacagctg
     2341 ggacttctgg gacgcattcc cgaaaccatg gatccggacg aggtgcccgt gctgattggc
     2401 gacattcgag cctcccgtca gctgaagctc gacggtgtgc atgcggtggc gttcgatcct
     2461 gaggtcgaca tcgcctttca tgcggacaag gttcccgtct ttcacaccaa tgccatggag
     2521 ttttctgtgt tcgcgggcga ctctctcctc tatcgacgcc gttactattc cgtgggcggc
     2581 ggctttatcg tggccgccag agaggatgat cccgagcagc ctgtaacgcc caaggcgttt
     2641 cagggcgtaa agacgaagcc ctatccctat cgtacgggcg acgagctgat gaaggtcgcc
     2701 agagacaaca atctgaccat tgccgagttg gtgtaccaaa acgagtgcgt tgaccgcacc
     2761 cccgaggagg tggaccgcag gctcgacgag gtctggcagg tgatgcacgc cgccgtcgag
     2821 cgcggcatgc gccagacgga ggtcctgccc ggtcccttcc ggattgctcg tcgagcgaat
     2881 gccctcatgc aggacgtgag gcagcgcacc gatgacccgc tcgccgtact ggactgggtg
     2941 aacgtctatg cgatggccgt ggccgaggag aatgcggcag gcggccgcgt cgtgacggcg
     3001 ccgaccaacg gtgcggccgg cgtgatccca gccgtcatcg agtactatct gcgtcacgtt
     3061 tcgggcagta ccatggaggg cgtcaggacc ttcctgctga cggcaggcgc cgtcggcatg
     3121 ctctacaagg aaaacgcttc catttccggt gccgaggtcg gttgtcaggg cgaggtcggc
     3181 gtggcctgtt ccatggcggc tgcgggtctg gcggccgtct gcggcggcac ggtcgagcag
     3241 atcgagaatg cggctgaaat cggcatggag cacaacctcg gcctgacctg cgaccccgtg
     3301 gccggtcagg tgcagattcc ctgcattgag cgcaacgcca ttgcggccgt caaggctatc
     3361 aacgcggcgc gcatggcact tcgcggcaac ggttcccact acatcagcct cgatcaggtg
     3421 attcgcacca tgatggcgac cggacgcgac atgcagagca agtacaagga aacgagtctt
     3481 ggcggtctgg ccgtttcgat tgtcgagtgc tgatgcatcg aaactgactt tcagggatct
     3541 tgaccgccat gctgatcggc gttttcttcg gcattgccgc gtgcatgatc tggggttgcg
     3601 tttacgtggc cccgctgatc ctgccggact acagtcctgt gcacatcgcg atgatgcgct
     3661 atctggtctt cggcatgctt gccgcaggcc tggcggtccc tgagtggcgt gagctgaagt
     3721 cctattcggc ggcggactgg cgtagggcgc ttgcactcgg tattgtcgga aacatcttct
     3781 actactggtt gctggccgag gcctgccaac gcgcaggagc gccgattgca ggcgccttca
     3841 cggcgctgat tcccatcatg gtgggcgtca tcggcaacat gagggcgggt gagagcgccg
     3901 tgccttggcg gcgcatcgtg ccgccactgt gcctgagctt cgcagggatg gtctgtctca
     3961 actggacgga gttcatctat ctggtggggg atggacgcgt gacctcgggc aacttctggc
     4021 ttggcgtagg ctttgcgttc ttctcctcat tggtctggac ctggtatccg ctcagcaacg
     4081 ccgaatggct gctgtcgcat cccggcaagt cgcccaagac gtggtcgacc gctcaggggc
     4141 ttgcactcct gccatgcgcc gccgtcggga tggctctgct tgcgggagac gggacgcagg
     4201 gggacattct cgggcccaga ccgtggatgt tcatcgcaac ggtggtgttt ctgggcgtct
     4261 gcgcctcctg gatcggcatc gtgctctgga gtttgatgag ccagaggctg acgcctgcgt
     4321 tgggcggcca gatgatcatc ttcgagacga tctttgctgt gacctacgcc cacttgtggc
     4381 gaagcgaatg gccgacgccg ctcatgctgg tcggtctggg gcttcttctg atgggggtta
     4441 cagcttccct caaggtttt
//
LOCUS       DBMDTI010000152         4424 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4658_length_4424_cov_3.548693, whole genome shotgun sequence.
ACCESSION   DBMDTI010000152 DBMDTI010000000
VERSION     DBMDTI010000152.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4658_length_4424_cov_3.548693"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="ACZPB6_07380"
     CDS             complement(<1..683)
                     /locus_tag="ACZPB6_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184323.1"
                     /translation="MAIDFTDEQVASTFRKLSVSHDSSANSRVGIYASWAALVLLAAC
                     FFVVAVFMIDADRRAESVRLQQSTDLIVQAVESRLMGTTELLQKSSMRLIHIPGTPSR
                     TAGAELAAMAFMQDRREVVDMALVDRDRKVLRHWASTSQSALMPDSPERHIDASAMYE
                     PVRSVLTREAPVVTSPYSVKDTRQVFFDLFVPTPTDDQVLMARINLSRLIADVANSVI
                     GTTPYIFYL"
     gene            837..1502
                     /gene="rpiA"
                     /locus_tag="ACZPB6_07385"
     CDS             837..1502
                     /gene="rpiA"
                     /locus_tag="ACZPB6_07385"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR1184324.1"
                     /translation="MALTQQELKREVARAALAYVEEGRILGVGTGSTVDQFIDQLPGI
                     REKIPACVSSSERSTKRLEALGFKVLDMNEVDEIGVYVDGADEIDPGFSMIKGGGGAL
                     TREKIVASISGRFVCIADASKKVPVLGSFPLPVEVIPMAARAVKAKLEALGATVKLRN
                     FVTDNGCHILDASGLRIEDPKALEAEINAWPGVVTVGLFAARGADVLLLGTQEGVQTF
                     ERG"
     gene            complement(1598..1876)
                     /locus_tag="ACZPB6_07390"
     CDS             complement(1598..1876)
                     /locus_tag="ACZPB6_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HIR1184325.1"
                     /translation="MARMVNCVKLKKEAEGLDYPPFPGALGARIWREVSKEAWAEWTR
                     LQTMMVNEYGLNLADLSARQHLMQQCERYFFGDAEGVQVPENFVPPKP"
     gene            2009..>4424
                     /gene="hrpA"
                     /locus_tag="ACZPB6_07395"
     CDS             2009..>4424
                     /gene="hrpA"
                     /locus_tag="ACZPB6_07395"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HIR1184326.1"
                     /translation="MSDRALFAAFQKVKAQVDDAVSLTREREARARAEEEKARAAKKT
                     RVVKAAETRRAEAGTASISASAFSATGGFGAALADAAKSSGASFRMAAPAKSRGGQPV
                     TAPKAEPSAVRKHAQMSPSTKAKEAQGKRHDAKADRAGRRPSGEQAGIRNERSEGTAR
                     QSGRTHSREGADRQKTVSSEAPKRRLSYEERRNPVPPFTLMPGLPVSERGEEVAKAIR
                     ENRVVIVCGETGSGKTTQLPKIAMMAGRGVHGMIAHTQPRRIAASSIAKRIAEELKTE
                     PGEVVGYKVRFTDTMSPGATIKLMTDGILLAETQNDPMLRAYDTVIIDEAHERSINID
                     FLLGYLKRLLSKRRDLKVIVTSATIDAERFARHFEIDGRPAPILTISGRTYPVEVRYR
                     PIEDMDEADDKTLVGAIDDAVGELEASGRGDILVFLPGEREIREAADVLRKSRPSTTE
                     ILPLFARLTAAEQERVFRPSGARRIVLSTNVAETSITVPGIRFVVDTGLARVKRYSYR
                     NKVEQLQIEPVSQASANQRAGRCGRVADGICIRLYSEDDFARRPAFTDPEILRSNLAA
                     VILRAISLKLGDVREFPFVQTPPPRAIADGFAILQELNAVTEEGELTRVGRELARLPV
                     DPRLGRMLLAGSEQGSLEELLTICAGLSIQDPRERPADAQQAADEAHRKLADEKSDFL
                     SYVKLWNWYEKANAEKESNRKLEAELHRRYLSVRRLREWRDVRRQLVQLTDELGWRRN
                     TSPATFEQVHRALLTGLLGNIGSKAVESDFRVPPYLGARGIKFWIWPGSARAKKAGRW
                     ILAAEIV"
BASE COUNT          826 a         1336 c         1476 g          786 t
ORIGIN      
        1 tcaagataga agatgtaggg cgtcgtgccg atcacgctgt tggctacgtc ggcgatgagg
       61 cgcgagaggt tgatgcgcgc catgaggacc tggtcgtcag taggagtcgg cacgaagagg
      121 tcgaagaaga cctggcgcgt atccttgacg gaatagggag aggtgacgac gggcgcctcg
      181 cgcgtgagta cggagcgtac gggttcgtac atggccgagg cgtcgatgtg gcgctcgggc
      241 gagtcgggca tcagcgcgct ctgcgaggtc gaggcccagt ggcgaagcac cttgcggtcg
      301 cggtccacga gcgccatgtc gaccacctct cggcggtcct gcatgaaggc catggcggcg
      361 agttcggcgc cagccgtgcg cgagggagtg cccggaatgt ggatgaggcg catgctcgac
      421 ttctgcagaa gctcggtcgt gcccatgagg cgggactcga cggcctgcac gatgaggtcg
      481 gtggactgct ggaggcggac gctttcggcg cggcggtcgg cgtcgatcat gaagaccgcg
      541 acaacaaaga agcaggcggc gaggagcacg agcgcggccc acgaggcgta gatgccgacg
      601 cgggagttgg cgctcgagtc atgcgagacg ctcagcttgc ggaaggtcga cgcgacttgt
      661 tcgtcggtga agtcaatggc catgtttttc gtctgggaag gtgctgtggg tgcattgtag
      721 tgcagagagg ggcgcaaaag gcgcgggaac cggtcaaggg agtacgctgc cggcgtcgaa
      781 aggccggcga ggacggtaaa ataggagccg ttcaccacca accggaattg tcccccatgg
      841 ctcttaccca gcaggaactc aagcgcgagg tcgcccgtgc ggcgctcgcc tatgtcgaag
      901 aaggccgaat cctcggcgtc ggaaccggct ccaccgtcga tcagttcatc gatcagctgc
      961 ccggcattcg tgaaaagatc cccgcctgcg tctcctcgag cgagcgctcc acgaagcgtc
     1021 ttgaagcgct cggcttcaag gtgctcgaca tgaacgaggt tgacgaaatc ggcgtctacg
     1081 tcgacggtgc cgacgaaata gatccgggct tctccatgat caagggaggc gggggcgcgc
     1141 ttacgcgcga aaagatcgtc gcgtccattt ccggccgctt cgtctgcatt gcggacgcct
     1201 cgaagaaggt gcccgtgctc ggttccttcc cgctccccgt cgaagtcatt ccgatggcgg
     1261 cccgtgccgt gaaggcgaag ctcgaggcgc tcggcgcgac cgtgaagctc cgtaacttcg
     1321 tcactgacaa cggctgccac attcttgatg cctcgggcct tcgcatcgag gatccgaagg
     1381 cgctcgaggc cgagatcaat gcctggcccg gcgtcgtcac ggtgggcctt ttcgccgccc
     1441 gcggcgccga cgtgctgctt ttgggcacgc aggaaggcgt gcagacgttc gaacgaggct
     1501 gacagaccgt tcgactgcaa ggaaaaagcc cgcacttcct tgggaagtgc gggcttttcg
     1561 ggccgtgttt cggcgaggac ctgtccgcag tcgcggatca gggcttcggc ggcacgaagt
     1621 tctcaggcac ctgaacgcct tcggcatcac cgaagaagta gcgttcgcac tgctgcatca
     1681 gatgctggcg ggcggacagg tcggcgaggt tgaggccgta ttcgttgacc atcatggtct
     1741 gaaggcgcgt ccattcggcc catgcctcct tgctcacctc acgccagatg cgggcgccga
     1801 gagcgccggg aaaaggcggg tagtcgagcc cttcggcttc cttcttgagt tttacgcagt
     1861 taaccatgcg ggccatctgt ttgctccttg attgatgtgt ggcggacggc atgaagccgc
     1921 ccgaagcgtt cgtacattgt agcgcgagtc cgtcaaattc tacgtggagc tctttctgag
     1981 gctccatcca tttattcgaa gattttctat gtccgatcgt gcgctgtttg cggctttcca
     2041 gaaggtgaag gcccaggttg atgatgccgt gtccctcacc cgtgaaaggg aggccagagc
     2101 cagagctgaa gaggagaagg cgcgtgcggc caaaaagact cgcgtcgtga aggcggccga
     2161 gacaaggagg gccgaggcgg gcaccgcgag catcagcgct tcggccttca gcgcaacggg
     2221 gggcttcggc gcggcgcttg cagacgcggc gaagtcgtcg ggtgcatcct tccgcatggc
     2281 ggcccccgca aagtcgcggg gcggacagcc cgtgacggcg ccgaaggcgg agccgtcggc
     2341 agtgcgaaag catgcgcaga tgtccccttc cacgaaggcg aaggaggcgc agggaaaacg
     2401 acacgatgcc aaagcggatc gagcaggccg caggccctcc ggtgagcagg caggcatcag
     2461 gaacgagcgt tccgaaggca ccgcaaggca aagcgggcgc acgcattcgc gcgagggagc
     2521 tgatcgtcag aagacggtgt cctccgaggc cccgaagcgc cgtctttcct atgaagagcg
     2581 ccgcaatccc gtcccgccct ttacgctcat gccggggctt cccgtttccg agcgtggcga
     2641 ggaggttgcg aaggccatcc gcgagaacag ggttgtgatc gtctgcggcg aaaccggctc
     2701 gggcaagacg acgcagttgc cgaaaatcgc catgatggcg gggcgcggcg ttcacgggat
     2761 gatcgcgcac actcagcccc gccgcatcgc ggcgagctcg atcgccaagc gcatcgccga
     2821 ggagctcaag accgagcccg gcgaagtcgt gggctacaag gtgcgcttca ccgacacgat
     2881 gtcgccgggc gccaccatca agctgatgac ggacggcatt ctgcttgccg agactcagaa
     2941 tgatccgatg cttcgcgcct atgacacggt gatcatcgac gaagcgcacg agagatcgat
     3001 caacatcgac ttcctgctcg gctatctgaa gcgcctgctt tcgaagcggc gcgacctcaa
     3061 ggtgatcgtc acttcggcca cgatcgacgc cgagcgattt gcccgacatt ttgagatcga
     3121 cgggcgtccc gctccgattc tgacgatctc gggtcgcacc tatcccgtcg aggttcgcta
     3181 tcgtccgatc gaggacatgg atgaggccga cgacaagacc ctcgtcggcg cgattgacga
     3241 tgcggtgggc gaactcgagg cttcgggacg cggggacatt ctggtgttcc tgccgggcga
     3301 gcgcgaaatc cgcgaggccg cggacgttct tcgcaagtcg cgtccctcga caacggagat
     3361 tcttccgctt tttgcgcgcc tgacggctgc agagcaggag cgcgtgttcc gcccctccgg
     3421 agcgaggcga atcgtgctct ccaccaatgt ggcggaaacc tccatcacgg tgcctggcat
     3481 caggttcgtc gtggacacgg ggcttgcccg cgtcaagcgc tactcctatc gcaacaaggt
     3541 cgagcagctt cagatcgaac ccgtgagtca ggcctccgcc aatcaacgcg ccggccgatg
     3601 cggccgcgtg gccgacggca tctgcatccg actgtacagc gaggatgact ttgcacgccg
     3661 ccccgccttt acggatcccg aaattctgcg ctcgaacctt gccgccgtca tcctgcgcgc
     3721 catttcgctc aagctgggcg acgtgcgcga atttccgttc gttcagacgc cgccgccccg
     3781 cgcgattgcg gacggttttg ccattctgca ggagctcaac gccgtcaccg aggaaggcga
     3841 actcacgcgc gtgggcaggg agctcgctag gcttcccgtc gacccgcgtc tcggccgcat
     3901 gctgcttgca ggctccgaac agggcagcct tgaagagctg ctgacgatct gcgcagggct
     3961 ctccattcag gatccgagag agcgtcccgc cgatgcacag caggcggcgg atgaggcgca
     4021 caggaagctt gcggacgaaa agtctgactt cctttcctac gtcaagctct ggaactggta
     4081 tgaaaaggcc aatgccgaga aggaaagcaa ccgcaagctt gaggccgagc ttcacaggcg
     4141 ctacctttcg gtgaggcgcc tgcgcgaatg gcgcgatgtt cgccgtcagc tcgtgcagct
     4201 caccgacgag ctcggctggc gcaggaacac gtcgcccgcc accttcgagc aggtgcaccg
     4261 cgccctgctg acgggtcttc tgggcaacat cggctctaag gccgtcgaaa gcgactttcg
     4321 tgtgcctccc taccttggcg ctcgcggcat caagttctgg atctggcctg gatcggcgcg
     4381 cgccaagaag gcgggtcgct ggattctggc tgccgaaatc gtcg
//
LOCUS       DBMDTI010000153         4413 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4672_length_4413_cov_1.954082, whole genome shotgun sequence.
ACCESSION   DBMDTI010000153 DBMDTI010000000
VERSION     DBMDTI010000153.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4672_length_4413_cov_1.954082"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            177..485
                     /locus_tag="ACZPB6_07400"
     CDS             177..485
                     /locus_tag="ACZPB6_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1184327.1"
                     /translation="MRPSFAVEESVRTLGANLKAARLRRRLPQSVVAERAGISLNTLS
                     KLENGDCGIAIGNVASVLQALGLGTPFSDVAAAGDDETGLMLEARRLPRRARQPKKEI
                     "
     gene            654..1784
                     /locus_tag="ACZPB6_07405"
     CDS             654..1784
                     /locus_tag="ACZPB6_07405"
                     /inference="COORDINATES: protein motif:HMM:NF019421.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HIR1184328.1"
                     /translation="MRFQYDGDWVESGVPLGADLPLEHGVLRPRLGKSAFGFLEDRAI
                     SAPVFSIFNDPASPLSGLPEGAGRLETTALLLPHRTDSLGALSVTPVDVDPLPERPRV
                     KRQSELPELIYAYHAMERGKAGSDEVSLLQNGLTLPGRRPVFTVERHREAQTARLRSM
                     LDPIDRPLWMHMALVAANRCGIRTVETNLEHEMGENILFTRRADRRGANADAPADKPL
                     LTLTGATLAARQESPRPVPPGYLALADILNGGGAAPKEDLPQMWRRIAFQLLTGGAGD
                     SLNRWQFHREPLGWRLSPAHTLEWNPSAQGRGLTMDGRRRLSCADDAIPYARYFGLTV
                     SDAKGILMEMRRILSGWEGLAEEFGADPRDIAYMAPLFEENL"
     gene            complement(1933..2583)
                     /locus_tag="ACZPB6_07410"
     CDS             complement(1933..2583)
                     /locus_tag="ACZPB6_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1184329.1"
                     /translation="MTEGNHEAGATDVTVRHVRRTRRICVHNATEIGALVRLARQRLG
                     MSQTDAAICCGVGRRFLVELENGKPTVQLDKMLVVLDALGIGLMVRGPGVSFTAEELA
                     QAAVKRESEDPPHVWEAEFSSGVDRPKRPDASEKPHRGRTPGTVALKYRRQSSVREEG
                     GTLVYETVDTNPVIAKKKEKHVEVCLRQPKRRNKSDDTSPVTDFGGSMRLDTNGRG"
     gene            complement(2769..3950)
                     /gene="metC"
                     /locus_tag="ACZPB6_07415"
     CDS             complement(2769..3950)
                     /gene="metC"
                     /locus_tag="ACZPB6_07415"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HIR1184330.1"
                     /translation="MKDLDAATQLVHAGRDWDPEWGCVNPPVVRASTVLHTSCEDMMD
                     RVHDEMACRDGKPCYGSYGGPAHKAFYSAMKTLEGPNAAGAWAFSTGLAATTTPLFAF
                     VRAGCHALFSDSIYGPTREFAEQILRTMGVDVEFFDPAASPEAIEAMMRPETTLLMME
                     NPGSHSFEMSDVPGIAAACRRHGVTSVIDNTWATPLYFKPLDHGVDMVVHAATKYIAG
                     HSDLLMGVVVCNERVWPAVYRTAVRLGQAASPDDVYLAYRGLHTMGVRVAHAAKSAER
                     IVHWLLERPEVETVRWPALESDPSHAIWKRDYTGATSLFAVVFRPEYDGRLAPFVDAL
                     KLFGRGYSWGGYESLLIPCYGTRSVRTSTFSRMVRIAVGLESPDDLIADLEQALKRLR
                     D"
     gene            complement(4108..>4413)
                     /locus_tag="ACZPB6_07420"
     CDS             complement(4108..>4413)
                     /locus_tag="ACZPB6_07420"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019441 - L-tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR1184331.1"
                     /translation="ADGVAHSPGWGLDALKYLFEERGVMAVGHETLDTDGSADVRARG
                     FLDCEKYVLESDHYQVEVMANLDKLPSTGGVIFVGLPKFDKFPGFPVCAWAVVPNED"
BASE COUNT          768 a         1494 c         1363 g          788 t
ORIGIN      
        1 aaaggcgtat aattcccatt attcgagatg ttagagcttc ggactgaact tcggattcat
       61 gcggaaaagg gcccgaaaac ggcgattttc cgccttttcg aacgaaaagc tcaaaaaaga
      121 ccttaactct cttttccttc atgtaaagtg gtttagttca caggattctg cgtgttatga
      181 gacccagttt tgcagttgaa gagtccgtca ggacacttgg cgccaacctc aaggcagcgc
      241 gtcttcgccg caggcttccc caatccgtcg tggcggagcg tgcgggcatt tcgctcaaca
      301 ccctctcaaa acttgaaaac ggcgactgcg ggatcgcgat cggcaacgtc gcctcggtcc
      361 ttcaggcgct cgggctaggc acccccttct ccgacgtcgc cgctgcgggc gacgatgaga
      421 cggggctcat gctcgaggca cgccgcctcc cccgccgggc ccgccagccg aagaaggaga
      481 tatgagcaaa aatgagaagc ccttccctgc gtctttatga gattcgtgcg cgctggctcc
      541 ccggcgaccc tctcgtcgga cgcctggccg tccctctttc aacaggaccc cgttcgggaa
      601 ctcttcaaga tccgggcgca tcgccggctc aggccgcccg cgccgcggcg gtgatgcgct
      661 ttcagtacga cggcgactgg gtcgagtcgg gcgtgccgct cggcgcggac ctcccgctcg
      721 agcacggcgt gctgcgtccg aggctcggaa agtcggcgtt cggttttctg gaagaccgcg
      781 cgatcagcgc ccccgtcttc tccatcttca atgaccccgc ctctccgctc tcgggccttc
      841 ccgaaggggc gggcaggctc gagacgaccg cactgcttct cccccacagg accgactcgc
      901 tcggggccct ttcggtgacg cccgtcgatg tcgatcccct gcccgagcgt ccgcgcgtca
      961 agcgccagtc ggagcttccc gaactcatct acgcctacca cgcgatggag cgcggcaagg
     1021 ctgggagcga cgaggtcagc ctgcttcaga acggcctcac gctccccggc cgccgtcccg
     1081 tcttcacggt cgagcggcat cgcgaggcgc agaccgcaag actgaggagc atgctcgatc
     1141 cgatcgaccg ccccctctgg atgcacatgg cgctcgtcgc cgccaaccgt tgcggcatcc
     1201 gcacggtcga gacgaacctc gagcacgaaa tgggcgagaa catcctcttc acgcgccgtg
     1261 cggaccgcag gggtgcgaat gcggacgccc ccgccgacaa gcccctcctg accctcacgg
     1321 gcgcgacgct tgccgcccgc caggagtccc cgagacccgt tccgcccggc tatctggcgc
     1381 tcgccgacat cctcaacggc ggcggcgcgg ccccgaagga ggacctcccc cagatgtggc
     1441 ggcgcatcgc cttccagctc ctcacgggcg gcgcgggcga ctcgctcaac cgctggcagt
     1501 tccacaggga gccgctcggc tggcgcctct cgcccgccca cacgctcgag tggaatccct
     1561 cggcccaggg ccggggcctc acgatggacg gccgcaggcg tctctcctgc gcggacgacg
     1621 ccattcccta cgcccgctac ttcgggctca ccgtgagcga cgccaagggc atcctcatgg
     1681 agatgcgccg catcctctcg ggctgggagg gactcgccga agaattcgga gccgacccga
     1741 gggacatcgc ctacatggcg ccgctcttcg aggaaaacct ttgagtagcc gcgggcctga
     1801 tgccgctccc ggcatccctc aggcgcattt ggagcgccgg aaatgacgaa gggcagaatg
     1861 cttcacggca ttctgccctt cttttgcgcc ggtgctgcgc ttttgctgcg gtcgcgcgtg
     1921 cggagttcga actcagccgc ggccgttggt gtccagacgc atcgagccgc cgaagtcggt
     1981 cacggggctc gtgtcatcag acttgttgcg gcgcttgggc tgtcgaaggc atacttcgac
     2041 gtgcttctcc ttcttcttcg cgatgacggg gttcgtgtcg accgtctcgt agaccagagt
     2101 ccctccttct tccctcacgg aggactggcg gcggtacttc agggcgacgg tgcccggcgt
     2161 gcggccgcgg tgcggcttct cggacgcatc ggggcgcttc ggacgatcga cgcccgacga
     2221 aaactccgcc tcccagacgt gcggcggatc ttcggactcg cgcttgacgg cagcctgcgc
     2281 aagctcctcg gccgtaaagg agacgccggg accgcggacc atgaggccaa tgccgagcgc
     2341 atccaggacg accaacatct tgtcgagctg cacggtgggc ttgccattct cgagctcgac
     2401 aaggaagcgg cggccgacgc cgcagcagat cgccgcatcc gtctgagaca tgcccagacg
     2461 ctggcgcgca agacgaacca gtgcgccaat ctcggttgca ttgtgcacgc agatgcgtct
     2521 ggtgcgccgg acgtgtcgca cggtgacgtc cgtcgctcct gcttcgtggt tgccttctgt
     2581 catagtctct tccctttatt gccgccgtca ccgtcgttcc gggtcttcgc gacagtgact
     2641 tgcgaaatat tttcacgata gcggaaacgg gggtccggca catcggaata gtctaggatt
     2701 atggctcatg ctacgatatt ttgcaaacaa aaaacgcagt gcatgatgcc tgacgttatg
     2761 cactcctgtc agtcgcgcag gcgtttgagc gcctgctcaa ggtctgcaat caagtcgtcg
     2821 ggtgattcca ggcccaccgc aatgcgcacc attcttgaaa aagtactcgt gcgtacagac
     2881 cttgttccgt agcacgggat gaggagcgac tcgtatcctc cccatgagta tccgcgccca
     2941 aagagcttca gcgcatcgac gaagggcgcg agcctgccgt cgtactcggg cctgaagacg
     3001 acggcgaaga gactcgtcgc acccgtgtag tcgcgcttcc agatcgcgtg cgaagggtca
     3061 gactcgagcg caggccacct gacggtctcg acctcggggc gctcgagcag ccagtgcacg
     3121 atccgttcgg cggactttgc cgcatgagcg acgcgcacgc ccatcgtatg aagccctctg
     3181 taggccaggt agacgtcgtc gggcgaagcc gcctgaccga gcctcacggc tgtacggtag
     3241 acggcgggcc agacgcgctc gttgcagacg acgacgccca tgagcaggtc ggagtggccc
     3301 gcgatgtact tggtggccgc gtgcacgacc atgtcgacgc catggtcgag cggcttgaag
     3361 tagagcggcg tcgcccaggt gttgtcgatg acgctcgtca cgccgtgcct gcggcaggcg
     3421 gcggcgatgc cgggcacgtc cgacatttcg aaagagtgcg aacccggatt ctccatcatg
     3481 aggagcgtcg tctcgggccg catcatggcc tcgatcgcct cgggcgaggc tgcgggatcg
     3541 aagaactcga cgtcaacgcc catcgtgcgc aggatctgct cggcgaattc gcgcgtgggt
     3601 ccgtagatcg agtccgaaaa gagcgcatga cagcccgccc gcacgaacgc aaagagcggc
     3661 gtcgtcgtgg cggccaggcc cgtcgaaaag gcccatgcgc ccgcggcgtt cgggccctcg
     3721 agcgtcttca tcgccgaata aaacgcctta tgtgcaggac cgccgtagct tccgtagcag
     3781 ggcttgccgt cccggcaggc catctcgtca tggacgcggt ccatcatgtc ctcgcaggac
     3841 gtgtgaagca ccgtcgaggc gcgcaccacg ggcggattga cgcagcccca ctccgggtcc
     3901 cagtcacggc ccgcgtgcac gagctgcgtt gcggcatcaa gatccttcat cgacacggac
     3961 tttcctcctt cttcgaatgc atgacaaaac ggtggtcaaa gcctgaactc cgccgccgtc
     4021 acccgccttc gggcgggatg cgttctcata ttctcgcacg cctgaacccg cgagaacccc
     4081 gagccgcact cagggcgtcc gccgcgctca gtcctcgttg ggcacgaccg cccatgcgca
     4141 gaccgggaag cccgggaact tgtcgaactt cgggaggccc acgaagatca cgccgcccgt
     4201 cgagggcagc ttgtcgaggt tcgccatgac ctcgacctga tagtgatcgg actcgagcac
     4261 gtacttctcg cagtcgagga atcccctcgc ccgcacatcg gccgaaccgt ccgtgtcgag
     4321 cgtctcgtgg cccaccgcca tgacgccgcg ctcctcgaag aggtacttga gggcgtcgag
     4381 gccccagccc ggagagtgcg cgacgccgtc ggc
//
LOCUS       DBMDTI010000154         4412 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4674_length_4412_cov_2.198794, whole genome shotgun sequence.
ACCESSION   DBMDTI010000154 DBMDTI010000000
VERSION     DBMDTI010000154.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4674_length_4412_cov_2.198794"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="ACZPB6_07425"
     CDS             <1..843
                     /locus_tag="ACZPB6_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1184332.1"
                     /translation="GVSEALLERMGGYTGCVGVLDTGRPGPVTTLRFDIDCLAIEETK
                     DENHLPNREGFASTRPGLSHACGHDGHTAVGLGVAQWLADNKETLCGKVKLVFQPAEE
                     GVRGAAAIAASGVLDDVDWFTAGHIGCDAKPGQVGVSHEGYLATTKFDLEIFGLPCAA
                     GSPEKGHSALMCACNTVMALGSLPGHSKGITRVQVGKIVSGTARNIVAGHAYVVLEVR
                     GESTEINDYMAANVETTVKGLAAAYECTYKLTRMGEAVTYKGDEEAVELVKAAARDAE
                     GAPT"
     gene            840..1040
                     /locus_tag="ACZPB6_07430"
     CDS             840..1040
                     /locus_tag="ACZPB6_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184333.1"
                     /translation="MNAKVGSEDCTMLIRRIQAHGGKCAFFYFGCDHHGHHRPDFEIQ
                     DRTSLPVGWGVFTGFVARTNAR"
     gene            complement(1158..1748)
                     /locus_tag="ACZPB6_07435"
     CDS             complement(1158..1748)
                     /locus_tag="ACZPB6_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1184334.1"
                     /translation="MKQYGFHFDATRCTGCKTCVVACCDKNGLEDGRQFRHVLAYENG
                     GWRQDRMTGAWHQDVTHGYVSVSCNHCDDPACVKVCPTKAHAKHDDKGGLVLIDPAKC
                     IGCGMCAKACPYGAPVLDEKAHKMTKCDACVDRVVKGELPVCVAACPQRALDFGYVDD
                     LKAKYGEGAHIEPLPSPAMTKPNLLVTHGKSAKVVK"
     gene            complement(1763..4207)
                     /locus_tag="ACZPB6_07440"
     CDS             complement(1763..4207)
                     /locus_tag="ACZPB6_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HIR1184335.1"
                     /translation="MLSRRHFLTNTAMLGSAAGFAATPLSSAVAAAVDDLETTTWSCC
                     SINCGSRCLLRCVSKKGRVIRIETDNTGDPEAGASATSCPQVRACQRGRSIRQRLYSE
                     ERLKFPMKRVGRRGEGKFERISWEQALDEIAARLKKTIAEHTNESIMLMHGSGSYSLF
                     NNRNCTFRFFNMIGGNIGWYSDYSAACIQHAWPYLYGGFGYGGVRSTNDGAGSYMNQV
                     KNAKLYVTFGNNPAVTRASGGGQSWELYGALREGKTRMVVIDPICTDTLAGRDAEWVA
                     IRPGTDAALCEGMAWVMINENLVDQAFLDKYCVGYDEKTLPKSAPKGSDYKSYILGQG
                     PDKTAKTPLWASRITQVPVETIERLAREIATTKPVFISQGWGPQRRMNGETQSTAIAM
                     MALLTGQVGLPGTNSGAREGDSYGIDTGLPTGKNPIGKSFPIFLWPRAVKDAKGMTAL
                     NAGVRGCDRLEHNIKFIWNTQGNTLVNQHGGVNQIREILEDEKLVETIVVVDNQMTPS
                     ARFADYVLPDTMNQEIDDMQGDAYAVGDYNYIVACPKAADILWEQKPNYWIMTEMAKR
                     FGLEEKFTEGRTQKEWIRWGYEQTLAKAAKQPDPIKLPGFDEFWAKGFVKYHMGRDSG
                     IVLKAFREDPEKHPLKTPSGRIEIYSERLAKIAETWELPKTKGQEIHPIPRFIATAEM
                     LGQGDPLEQKYPLEVYGYHGAGRTHSTYHNVPWLRALHPDELMINPIDAEPRGIKTGD
                     KVRVFNDRGSLVIPAFVTARVIPHLVAMPQGAWYRPDEKGVDQGACFNVLTTLEATPL
                     AKGNPSHTNLVQVEKI"
BASE COUNT          762 a         1438 c         1378 g          834 t
ORIGIN      
        1 ggcgtctccg aagccctcct cgagcgcatg ggcggctaca cgggctgcgt cggcgtgctc
       61 gacacgggcc gtcccggtcc cgtcaccacg cttcgcttcg acatcgactg cctcgccatc
      121 gaggagacga aggacgaaaa ccatctgccc aaccgcgagg gcttcgcctc cacgcgtccc
      181 ggcctctccc acgcctgcgg gcacgacggc cacacggccg tgggccttgg cgtcgcgcag
      241 tggctcgccg acaacaagga gaccctctgc ggcaaggtga aactcgtctt ccagcccgcc
      301 gaggaaggcg ttcgcggggc agccgccatc gccgcttcgg gcgttctcga cgacgtcgac
      361 tggttcaccg ccggccacat cggctgcgac gcgaaacccg gccaggtcgg cgtctctcac
      421 gaaggctatc tcgccaccac gaagttcgac ctggagatct tcggccttcc ctgcgcggcg
      481 ggcagtcccg aaaagggcca cagcgccctc atgtgcgctt gcaacaccgt catggcgctc
      541 ggcagcctgc ccggccacag caagggcatc acccgcgtgc aggtcggcaa gatcgtctcg
      601 ggcacggccc gcaacatcgt cgcaggccac gcctacgtcg tcctcgaggt ccgaggcgag
      661 tcgaccgaaa tcaacgacta catggccgcc aacgtcgaga cgaccgtcaa gggcctcgcc
      721 gcagcctacg agtgcacgta caagctcacc cgcatgggcg aggccgtgac ctacaagggc
      781 gacgaggagg cggtcgagct cgtcaaggcc gccgcccgcg acgccgaagg cgcaccgaca
      841 tgaacgccaa ggtcggcagc gaagactgta ccatgctcat ccgccgcatc caggcacacg
      901 gcggcaagtg cgccttcttc tacttcgggt gcgaccacca cggtcatcac cgccccgact
      961 tcgagattca ggaccgcacc tcgctccccg tcggctgggg cgtcttcacg ggattcgtcg
     1021 cgcgcaccaa tgcacgatga cacccatcac gcctgaatga ccgaaggccc gccggaatca
     1081 aagttccggc gggccttctt ttcaaaagaa tcccgggaag gactccgcat tccggaagcc
     1141 cttcccttcc gactctccta cttcacgacc tttgcggact tgccgtgcgt cacgagcagg
     1201 ttgggcttcg tcatggcggg agacggcagc ggctcgatgt gcgcgccttc gccgtacttc
     1261 gccttgaggt catcgacata cccgaagtcg agtgcacgct gcgggcaggc cgcgacgcag
     1321 acgggaagct cgcccttgac gactcggtca acgcaggcgt cgcacttcgt catcttgtgc
     1381 gccttctcgt cgagcacggg tgcgccgtag gggcaggcct tcgcgcacat gccgcaccca
     1441 atgcacttgg caggatcgat gagcacgagt ccgcccttgt cgtcgtgctt cgcatgcgcc
     1501 ttggtcgggc agaccttcac gcatgcggga tcgtcgcagt gattgcacga gacggagaca
     1561 tagccgtggg tcacgtcctg atgccaggcg cccgtcatgc ggtcctgacg ccaaccgccg
     1621 ttttcataag cgaggacgtg gcggaactgc ctgccgtcct caagcccgtt cttgtcgcag
     1681 cacgccacca cgcaggtctt gcatccggtg cagcgcgtcg catcgaaatg gaatccgtat
     1741 tgcttcatga tctgcctccg catcaaatct tttcaacctg cacgaggttc gtgtgggaag
     1801 gattgccctt ggcgagcggc gtggcctcga gcgtcgtgag gacgttgaag cacgcgccct
     1861 gatcgacgcc cttttcatca ggcctgtacc aggcgccctg aggcatcgcc acgagatgcg
     1921 ggatcacgcg ggcggtgacg aaggccggaa tgaccagcga gccgcggtcg ttgaagacgc
     1981 ggaccttgtc gcccgtcttg atgccgcgcg gttcggcgtc gatcggattg atcatcagct
     2041 cgtcgggatg cagcgcacgg agccacggta cattgtggta ggtcgagtgc gtgcggcctg
     2101 ctccgtgata gccgtagacc tcaagcggat acttctgctc gagcggatca ccctggccga
     2161 gcatttcggc cgtcgcgatg aagcgcggga tcggatggat ttcctgacct ttggtcttcg
     2221 ggagctccca ggtctcggcg atcttcgcca gacgctcgga gtagatctcg atcctgcccg
     2281 aaggcgtctt gagcggatgc ttttccggat cctcgcggaa ggccttgagc acaatgccag
     2341 aatcgcgccc catgtggtac ttcacgaagc ccttcgccca gaattcgtca aagcccggga
     2401 gcttgatcgg atcgggctgc ttggcggcct tggccagcgt ctgctcgtaa ccccagcgga
     2461 tccattcctt ctgcgtgcgg ccttcggtga acttctcttc gagtccgaag cgcttcgcca
     2521 tttcggtcat gatccaatag ttgggcttct gctcccagag gatgtcggcg gccttcggac
     2581 aggcgacgat gtagttgtag tcgccgaccg cataggcgtc gccctgcatg tcgtcgattt
     2641 cctgattcat cgtgtcgggg agcacgtagt ccgcaaagcg tgcggaaggc gtcatctggt
     2701 tgtcgaccac gacgatcgtc tcgacgagct tctcgtcctc gaggatctcg cggatctggt
     2761 tgacgccgcc gtgctggttg acgagcgtgt tgccctgggt gttccagatg aacttgatgt
     2821 tgtgctcgag cctgtcgcag ccgcgcacgc ccgcattcaa cgcggtcatg cccttcgcgt
     2881 ccttcacggc gcgcggccag aggaagatcg ggaaggactt gccgatcggg ttcttgcctg
     2941 tcggcagtcc cgtgtcgatg ccgtaggagt cgccctcgcg tgcgccggag ttggtgccgg
     3001 gcaggcccac ctgacccgtg aggagcgcca tcatggcgat cgcggtcgac tgggtctcgc
     3061 cgttcatgcg gcgctgcgga ccccagccct gcgagatgaa gacgggcttc gtcgtggcga
     3121 tctcgcgggc aaggcgttca atcgtctcga ccggcacctg cgtgatacgg gaggcccaga
     3181 gcggcgtctt ggccgtcttg tcgggaccct gacccagaat gtaggacttg tagtccgagc
     3241 ccttcggagc ggacttcggg agcgtctttt cgtcgtagcc cacgcagtac ttgtcgagga
     3301 aggcctgatc gacgaggttc tcgttgatca tcacccaggc catgccttcg cagagcgcgg
     3361 cgtcggtgcc cggacggatg gcgacccatt cggcgtcacg gcccgcgagc gtgtcggtgc
     3421 agatcggatc gatgacgacc atgcgggtct tgccttcacg gagcgcgccg tagagctccc
     3481 acgactgacc gccgcccgaa gcgcgcgtga ccgcgggatt gttgccgaag gtgacgtaga
     3541 gcttcgcgtt cttcacctga ttcatgtacg agcccgcgcc gtcgttcgtc gagcgcacgc
     3601 cgccgtagcc gaagccgccg tagaggtagg gccaggcatg ctgtatgcat gcggccgaat
     3661 agtccgaata ccagccgatg ttgccgccga tcatgttgaa aaaccggaac gtgcagttgc
     3721 ggttgttgaa gagcgaatag ctgcccgagc cgtgcatgag catgatcgat tcgttggtgt
     3781 gctcggcaat cgtcttcttg agccttgcgg cgatctcgtc gagcgcctgt tcccaggaaa
     3841 tgcgctcgaa cttcccttcg ccgcggcgcc cgacgcgctt catcgggaac ttcaggcgct
     3901 cttccgaata gaggcgctgg cgaatcgaac gccccctctg gcaggcacgc acctgcgggc
     3961 aggacgtcgc ggaggcgccg gcctccggat cacccgtgtt gtcggtttcg atgcggatga
     4021 cgcggccctt cttcgagacg cagcgcaaga ggcagcgaga gccgcagttg atcgaacagc
     4081 acgaccaggt cgtcgtctca agatcgtcga ccgcagccgc aacggcgctg ctcaagggcg
     4141 tggccgcaaa cccggcggca gagccgagca tggccgtatt tgtcagaaaa tgacgtcgtg
     4201 aaagcatgct tctcatctcc tcaaaaagtc atgcagggaa gagtcctgaa tcctcgggcg
     4261 gctgcggggt ccaatgcagc cgtgcgccct ttccctcggg acctcccatt ctaaacgcgc
     4321 ccgaatgact cgggcactcg tttgagtata ggaccgtcgc ccatgagcga catgagcatt
     4381 tctcccatcc tcctctctgc gaaagaggct aa
//
LOCUS       DBMDTI010000155         4411 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4676_length_4411_cov_2.528770, whole genome shotgun sequence.
ACCESSION   DBMDTI010000155 DBMDTI010000000
VERSION     DBMDTI010000155.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4676_length_4411_cov_2.528770"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="ACZPB6_07445"
     CDS             complement(<1..403)
                     /locus_tag="ACZPB6_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184336.1"
                     /translation="MSIQEQNTTRLSNQNTLHNSPKKAPDSTPQHDAKMRRRLFAARA
                     GFFTGGFTVASWAPIIPFVQTELSLEPAVLGTLLFGLGIGSFFGMPIAGTVSTRFGNR
                     AALAASGSSESASVIESIRALKDEIESVRAES"
     gene            563..1462
                     /locus_tag="ACZPB6_07450"
     CDS             563..1462
                     /locus_tag="ACZPB6_07450"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1184337.1"
                     /translation="MTISIEEMRSFAAVAAQGTFSAAARALGRSQSVVSTHIAGMENE
                     LGIRLFVRGTPAKLTPAGEELLPGALRVLREAERFESRASELFRLPEPLLYMGIDMAL
                     ELPLLLDLLRDFSRQFPSARLQLENISSSESNWFFRRSHMTMAVIFSSRPDPECEEHL
                     IGLAPRAVAVSKKHPLAAVERPSIEALQGVRQIVVNARDSESESPITVSPDVWEVDGA
                     QWAIGLAARGVGWTIVPRAVAMTQPGLRSSLALLSAPFDLPPERLILRTKKDEVPPVF
                     AKWWAAALRKTARRLGLVQPESM"
     gene            1684..2178
                     /locus_tag="ACZPB6_07455"
     CDS             1684..2178
                     /locus_tag="ACZPB6_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184338.1"
                     /translation="MKTLLLKDSSLRVSVMSFHHEIVYSVMRAYFEALVSHVLIWNER
                     SYGLLWNTEMVEDISERLRFFSKDTKRWRVYAVLFGVINFRRELRIVPFRDALEEHMS
                     TGLNVSAVAVIDVHRIYETFGTKNVPEDVLEDFVNKYNAWHNSFEWFVRLFENGRLKS
                     ENRV"
     gene            2185..2733
                     /locus_tag="ACZPB6_07460"
     CDS             2185..2733
                     /locus_tag="ACZPB6_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184339.1"
                     /translation="MDDVIMWLKHQPELVNVRITPKQFADARADVESDFGILVKKNFV
                     LTGINALNRLEFRDGAMALRVAFDDLVRVHSELHDPESIGSVFWSADSVVALLANGIG
                     FSIKRDDMGFVRISAPVGTPLHIVDGLRNMLLAFQGAAPDRIEIAESDDISVQSFIDG
                     EGAIPTLNECHRALCEAIGRIR"
     gene            complement(2805..3854)
                     /locus_tag="ACZPB6_07465"
     CDS             complement(2805..3854)
                     /locus_tag="ACZPB6_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024634.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1184340.1"
                     /translation="MTGAVRLIAGRCVRERHAGAKCTACADACPARALSFEGAALRII
                     APACEGCGACAAACPRDALELPGAGWREAVAAVPSSGVLECRCSHAAQGPGTPVPCIG
                     GLCATTRLELLRGGLRTLRIATGVCEGCPAEVRCEGVTLRNAFVRDLREMAGAFGRTV
                     DVVFSTEPVPEVDGGRRHLLGIVVRKSAEPVSPERIAGHAPDKSAGHLLVTEGSRRRL
                     MAARGLLSGMQDAEGVRAAKAACLAGLRVPQFDSEKCTACGLCAAACPQYALSAKTEG
                     EGFTIAAVETACTGCGLCADICTSKAVSFGAPDSADAWLSGRPVVKLAVKGRRRASAW
                     EGIVERSFTSGYFNR"
     gene            complement(3851..>4411)
                     /locus_tag="ACZPB6_07470"
     CDS             complement(3851..>4411)
                     /locus_tag="ACZPB6_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HIR1184341.1"
                     /translation="LSIRKPGIVQPIITIPDSLTLTETLDLFKKESSDFAVVINEYGL
                     TVGIITLKDILWPVMGNYDVWADDQLLVERADGTWLVDGSTPVDDIEREFGIESMPEE
                     ETYETVSGFIMYMLRRVPKRGDFVEYAGYRFEVTNMDGFHIDQVLMRRADHHVKHADK
                     AEKVEKAPEGGADASAADQINPGGTK"
BASE COUNT          781 a         1453 c         1381 g          796 t
ORIGIN      
        1 ccgattcagc gcgcacggac tcgatttcgt ctttcaaggc gcgtatcgat tctatgacgg
       61 aagcagactc gctcgacccc gaggcggcaa gcgccgcgcg attgccgaag cgcgtcgaga
      121 ccgtgcccgc aatcggcatg ccgaagaatg agccgatgcc gaggccgaag agcagcgtgc
      181 cgagcaccgc gggttcgagc gaaagctcgg tctgcacgaa gggaatgatg ggcgcccagc
      241 tcgcgacggt gaagccgccg gtgaagaagc ccgcgcgcgc ggcgaagagc ctgcggcgca
      301 tcttcgcatc atgctgcggc gtgctgtccg gagccttctt cgggctgttg tgaagggtgt
      361 tctgattgga aagacgagtg gtgttttgtt cttgtatcga cataaggacc tgcgtgggta
      421 gtcaggacac cgtcattttc tcattcaaaa ttcgatctct caagatggat tccatcggat
      481 ttttgataga ctgaaaccgc catcgcccgt cctgggcgga cttcgggcat tcggtcccat
      541 tcaaaacgga gcgttttcag tcatgacgat ttcgatcgaa gagatgagga gctttgccgc
      601 cgttgcggcg cagggcacct tttcggccgc ggcccgtgct ctcgggcgca gccagtcggt
      661 ggtctcgacc cacattgcgg gcatggagaa cgagctcggc atcaggcttt tcgtgagagg
      721 cacgcccgcg aagctcaccc ctgcgggcga ggagctcctg cccggcgccc tgcgggtgct
      781 cagggaggcc gagcggttcg agagccgcgc ctcggagctc ttcaggctgc ccgagcccct
      841 tctctacatg ggcatcgaca tggcgctcga gctgccgctt ctccttgacc tgctgcgcga
      901 cttctcgcgc cagttcccgt cggcgcgcct gcagctcgag aacatctcga gctcggagtc
      961 gaactggttc ttccggcgtt cgcacatgac gatggcggtc atcttctcct cgaggcccga
     1021 ccccgagtgc gaggagcatc tcatcggact cgcgccccgc gcggtggcgg tctcgaagaa
     1081 gcatccgctc gcggcagtcg agcgcccctc gatcgaggca cttcagggcg tgcggcagat
     1141 cgtcgtcaac gcccgcgaca gcgagtcgga gtccccgatc accgtgagcc ccgacgtctg
     1201 ggaggtcgac ggcgcacagt gggcgatcgg gcttgccgcc agaggcgtgg gctggacgat
     1261 cgtgccccgc gccgtcgcga tgacgcagcc gggcctgcgc tcctctctcg cgctcctctc
     1321 ggcccccttc gacctgccgc ccgagcgcct catcctgcgc accaagaagg acgaggtccc
     1381 acccgtcttc gcgaagtggt gggccgccgc cctcagaaag accgcacgcc gcctcggact
     1441 cgttcagccc gaatcaatgt gacgatttcc gcccgcctct gtgacgggcg gtctgaaaac
     1501 gagcgcggga tcgggacttt cccgtctttt ttgcaagaaa atgcgcgttt cgggcacgtt
     1561 ttcgacacaa tgacgctcaa aacaggctaa agtgttttca agccggtttc tttcggatcc
     1621 acttgtcaaa ttgaagtttt caataaaacg gcacttcaac gcggcacaac acggttttcc
     1681 accatgaaaa cgctccttct caaagacagt tcgctgcgcg tttcggtgat gagctttcat
     1741 cacgagatcg tctacagcgt gatgcgcgcc tacttcgagg cgctcgtcag tcacgtcctc
     1801 atctggaacg agcgttccta cggactcctg tggaacaccg agatggtgga ggacatctcc
     1861 gagcggcttc gcttcttctc caaggatacc aagcgctggc gcgtctatgc agtcctcttc
     1921 ggcgtcatca actttcgccg cgaactgcga atcgtgccct tcagagatgc gcttgaggag
     1981 cacatgagca cggggctcaa cgtctcggcc gtcgccgtga tcgacgtgca ccgcatctac
     2041 gagaccttcg gcacgaagaa cgtgcccgag gacgtccttg aggacttcgt caacaagtac
     2101 aacgcctggc acaactcgtt cgagtggttc gtgcgccttt ttgaaaacgg ccgcctcaag
     2161 agcgagaacc gcgtctgacg cacgatggac gacgtgatca tgtggctcaa gcaccagccc
     2221 gagctcgtca acgtgcgcat cacgcccaag cagtttgccg acgcgcgagc cgacgtcgag
     2281 tccgacttcg gcattctcgt gaagaagaac ttcgtgctca caggcatcaa cgccctcaac
     2341 agactcgagt tccgcgacgg cgccatggcg ctgcgcgtcg ccttcgacga cctcgtgcgc
     2401 gtgcactccg aactgcacga ccccgaatcg atcgggagcg tcttctggag cgccgacagc
     2461 gtcgtggcgc ttctcgccaa cggcatcggc ttcagcatca agcgcgacga catgggattc
     2521 gtgcgaatct ccgcccccgt gggcacgccg ctgcacatcg tcgacggtct caggaacatg
     2581 ctcctggcct tccagggggc cgcacccgac cgcatcgaga tcgccgagtc ggacgacatc
     2641 tccgtgcaga gcttcatcga cggtgaaggc gccatcccca cgctcaacga gtgccaccgc
     2701 gcgctctgcg aagcgatcgg ccggatccgc tgacgcccct gacgaagcct cacaaaagga
     2761 agggcctgca tcttccggat gtcaggcccc cttttcttgt tcagttatcg gttgaagtat
     2821 cccgacgtga agcttcgctc cacgattccc tcccaggcgg acgcgcgcct tctccccttc
     2881 acggcgagct tgacgaccgg tcttcccgaa agccaggcgt cagccgagtc gggcgcaccg
     2941 aaggagaccg ccttcgacgt gcagatgtcg gcgcagaggc cgcagcccgt gcaggccgtc
     3001 tcgacagccg cgatcgtgaa gccttcgccc tcggttttcg ccgagagcgc gtactgggga
     3061 caggccgcgg cgcagagccc gcaggccgta cacttttccg aatcgaattg aggcaccctg
     3121 aggccggcaa ggcacgctgc cttcgcagcg cgcacgcctt cggcatcctg catgcccgaa
     3181 agaagccccc ttgccgccat gagcctgcgc cttgagccct ccgtcacaag caggtggccc
     3241 gcggatttgt cgggcgcgtg ccccgcaatc ctttcaggcg aaacgggctc ggcggacttt
     3301 ctgacgacga tgcccagaag atgacggcgg ccgccgtcga cctcgggaac gggctcggtg
     3361 ctaaagacga cgtcaaccgt gcgcccgaag gccccggcca tctccctgag atccctgacg
     3421 aatgcgttcc gcagcgtgac gccttcgcac cgcacctcgg cgggacagcc ctcgcagacg
     3481 cccgtcgcga tcctgagcgt gcgaagcccg ccccgaagaa gctcgagcct cgtcgtggca
     3541 caaaggcctc cgatgcaggg aacgggcgtg cccggcccct gagcagcatg actgcagcgg
     3601 cattcgagaa cgcctgagga aggcacggcc gcaacggcct cgcgccagcc cgccccgggc
     3661 agctcgagcg cgtcgcgcgg acaggccgcg gcacaggcgc cgcaaccctc gcaggcgggc
     3721 gcgataatgc gaagcgcggc cccttcgaac gagagggcgc gtgcgggaca ggcgtcggcg
     3781 caggcggtgc atttggcgcc cgcgtgccgc tcacgcacgc agcggcccgc aataaggcga
     3841 acggcgcccg tcacttcgtg ccgcccggat tgatctggtc ggcggcggat gcgtcggcgc
     3901 cgccctcggg ggccttctcg accttctcgg ccttgtcggc gtgcttgacg tggtggtccg
     3961 cacgacgcat gagcacctgg tcgatgtgga agccgtccat gttggtgacc tcgaagcggt
     4021 agcccgcata ctcgacgaag tccccgcgct tgggtacgcg gcggagcatg tacatgatga
     4081 agcccgagac cgtctcgtag gtctcctcct caggcatgct ctcgatgccg aactcgcgct
     4141 cgatgtcgtc gacgggcgtc gagccgtcaa cgagccaggt gccgtcggcg cgctcgacga
     4201 ggagctggtc gtcggcccag acgtcgtagt tgcccatcac gggccagagg atgtccttga
     4261 gggtgatgat gccgaccgtg aggccgtatt cgttgatgac gaccgcgaag tcggagctct
     4321 ccttcttgaa gaggtcgagc gtctcggtga gcgtgagcga gtcggggatc gtgatgatgg
     4381 gctgcacgat gccgggcttg cggatcgaga g
//
LOCUS       DBMDTI010000156         4403 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4689_length_4403_cov_2.383543, whole genome shotgun sequence.
ACCESSION   DBMDTI010000156 DBMDTI010000000
VERSION     DBMDTI010000156.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4689_length_4403_cov_2.383543"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="ACZPB6_07475"
     CDS             <1..231
                     /locus_tag="ACZPB6_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer system coupling protein
                     TraD"
                     /protein_id="HIR1184342.1"
                     /translation="TFGKTTVWSTSASLSSSADGSPVPDFRASVTRSIQGRRDEVVPP
                     DVLGRLPNTEFFASVSGGRLYKGRIPILLSDD"
     gene            262..888
                     /locus_tag="ACZPB6_07480"
     CDS             262..888
                     /locus_tag="ACZPB6_07480"
                     /inference="COORDINATES: protein motif:HMM:NF025706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncC plasmid conjugative DNA transfer DtrJ
                     family protein"
                     /protein_id="HIR1184343.1"
                     /translation="MMKYMLTATALVLILMVMWLPARTPPEIFEETALREALMLRTGE
                     GIPMPETAANLAVEYLDGLVWVSGVPDRYTLRPYGGSPMTKDEFAEIGRAAERTTQTE
                     WLRAMKSLFVMATQRLAVMTAGTIALMPFILALIVDGFVRRRIREAEYHAPSPTWWTA
                     SLSLQLLAVCLGIVAIMHPNYPPQWLSVFPVAFALAARMTAATWHRFI"
     gene            complement(963..1604)
                     /locus_tag="ACZPB6_07485"
     CDS             complement(963..1604)
                     /locus_tag="ACZPB6_07485"
                     /inference="COORDINATES: protein motif:HMM:NF037679.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1184344.1"
                     /translation="MSEIENDAAEDVSYEDNEKHAVRRRANGRATRLKILAAARAIII
                     EKGTDSLSIDRVIKEAGVSKGSFMYHFPSRQALIEALVNEYAAHLGEVQTGLTNKAKS
                     DCPMLEAYAEWYKGFTSGEIDSGSSPLVALAMASRENRKFMEPVRDWYRRYFDRVKQE
                     ACGSERALVYTLAYDALFFHHLFGTDVLTDDEKKAVTRTLQAFADGGVNKQET"
     gene            1822..3372
                     /locus_tag="ACZPB6_07490"
     CDS             1822..3372
                     /locus_tag="ACZPB6_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HIR1184345.1"
                     /translation="MQKREFLKGSLAALGVFGCGAAAARSEFPEARDIKWDETFDVVV
                     VGSGIAATCAANESLDLGAKRVIMVDKMPVFGGNSAITGFWINVPRTPEQLAHGVKDD
                     SPELFLKDTLKAGEYFNDPKLAHALCFKALESFEYIRKLGCEFAPTPFIQGGHSKIRS
                     LAPKVSAGISVMLPLHRHFLKRGGIMRKNTIVDEIIKDETGRCVGVAVRENYVFDINS
                     RDNDLTNKTGVRKYYRAEKGIVFGAGGFVNDWRMCSLIDPKITKETEGTNHPGATAGA
                     LFTLMRAGAIPVHIDYIQWGPWCSPDDKGIGMGADVCDYIKQYGFAIDPKTGRRCFNE
                     LGNRTELTNDIFSLKNEDGSQRFAVLIADSQFVPKVSIDRYLSKSLKSGTTKKFETLE
                     ELAKFYGLPLKELKDEMAKYNGWIKEGREFDPDFNRPLTPNEGVLMGKGPWYGHRLTS
                     KLHYTMGGARINEKAEVIGQDFKPIPGLYAAGEITGGVHGVTRLGGNSVLDGIVFGRI
                     AAQSIMAK"
     gene            3393..3728
                     /locus_tag="ACZPB6_07495"
     CDS             3393..3728
                     /locus_tag="ACZPB6_07495"
                     /inference="COORDINATES: protein motif:HMM:NF025890.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR1184346.1"
                     /translation="MKKLVVIAMAALFTAGAYAADVAIPTSAKHKQDCTMCHQDGFKP
                     VPTEKCLTCHADINKGSYTLANGKENPHVSIHYLPETVDCNLCHREHGKSQNYCQACH
                     ADGLGFKVP"
     gene            4068..4361
                     /locus_tag="ACZPB6_07500"
     CDS             4068..4361
                     /locus_tag="ACZPB6_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184347.1"
                     /translation="MLLTDNDRNALEACDENGVTNVGRIFALLDNFVEEGVQQGRFSR
                     EEADADLEIALWRSFALLQCDDYVSYAQAVEVLQKAEAAAHQILHILDGMLVA"
BASE COUNT          892 a         1408 c         1222 g          881 t
ORIGIN      
        1 acgttcggga agaccaccgt ctggtcgacc tccgcttcgc tctcctcatc ggccgacggc
       61 tctcccgttc cggactttcg cgcctccgtc acgcgcagca tccaggggcg gcgtgacgaa
      121 gtcgtcccgc ccgacgttct cggccgactg cccaacaccg agttcttcgc aagcgtctcg
      181 ggcggcaggc tctacaaggg taggattccg atccttctct ccgacgactg accgcatcca
      241 ttcaaatccc tttggaacaa aatgatgaag tacatgctga ccgcgaccgc gcttgttctc
      301 atcctcatgg tgatgtggct gccagccaga accccgcccg agatcttcga ggagacggcc
      361 ttgagagagg cgctgatgct gagaacgggc gaggggattc ccatgcccga gacggccgcg
      421 aatctggcgg ttgaatatct cgacgggctc gtctgggtct cgggcgtccc cgaccgctac
      481 acgcttcgac cctacggcgg ctcaccgatg acgaaggacg agtttgccga gatcggcagg
      541 gccgccgagc gtacgactca gaccgagtgg ctgcgtgcca tgaagagcct cttcgtgatg
      601 gcgacccagc gccttgccgt catgaccgcg ggcaccatcg cactcatgcc cttcattctt
      661 gcgctgattg tcgacgggtt cgtacgtcgc cgcatccgcg aagccgagta ccacgcgccg
      721 agtcccacct ggtggaccgc ttccctctcc ctgcagctcc ttgcggtctg cctcgggatc
      781 gtggccatca tgcatcccaa ctatccgcct cagtggcttt ccgtctttcc cgttgcgttc
      841 gccctggccg ctcgcatgac ggcagcgacc tggcacaggt tcatttgagt ttcccttgga
      901 attccccgtc caatgagatg tctcaacgtt gggcgggatt tccttttcca gaaaacccgg
      961 cctcaggtct cctgcttatt cacgcctcca tcggcgaagg cctgcagcgt tctcgtcacg
     1021 gccttctttt catcatccgt cagcacgtcc gttccaaaga gatggtggaa gaagagggca
     1081 tcgtaggcga gggtgtagac cagagccctc tcggagccgc aagcctcctg cttgacgcga
     1141 tcgaagtagc gcctgtacca gtcgcgcaca ggctccatga acttccggtt ctctcttgac
     1201 gccatggcga gtgcaacgag cggcgaagag ccgctgtcga tttctcccga cgtgaagccc
     1261 ttgtaccact cggcataggc ctcgagcatc ggacaatcgc tcttcgcctt gttggtgagt
     1321 cccgtctgaa cctcgccgag atgcgctgcg tattcgttga cgagcgcctc aatcagtgcc
     1381 tgccggcttg ggaagtggta catgaagctt cccttggaga ctcccgcctc cttgatcacg
     1441 cggtcgatgg agaggctgtc agtgcctttt tcaatgatga tcgcgcgagc tgcagcgagg
     1501 atcttgagcc tcgtcgcgcg gccgtttgcc ctgcgcctca cggcgtgctt ttcgttgtcc
     1561 tcgtaggaaa cgtcttcagc ggcgtcgttt tcaatctcac tcattttctt gcatctccag
     1621 tcgtcatttg caaagggcgt ctcagactgc atagtctaaa ccaatcgggg ttaaccctgt
     1681 tggtgaaaga cccgatgggc gtcctcttgt ccttgttgcc gtcttaagta tacagtacgg
     1741 acggtactaa taggcgtccg taagacctca gcggtccatg ggttcatggg cagcagcgga
     1801 tgcatgaagg agaactcaaa aatgcagaaa cgcgaattcc tcaagggcag ccttgctgct
     1861 ctgggcgtct tcggttgcgg cgctgccgcc gccaggtccg aattccccga agccagggac
     1921 atcaagtggg acgagacgtt tgacgttgtc gtggtgggct cgggcattgc cgccacgtgc
     1981 gccgccaacg aatcgctcga cctcggcgcg aagcgagtca tcatggtcga caagatgccc
     2041 gtcttcggcg gcaactccgc catcacgggc ttttggatca acgtcccgcg cacgcccgag
     2101 cagctcgctc acggcgtcaa ggacgacagt cccgaactct tcctcaagga cacgctcaag
     2161 gccggcgaat acttcaacga cccgaagctc gcccatgcgc tgtgcttcaa ggccctcgag
     2221 tccttcgaat acatccgcaa gctcggctgc gaatttgcgc ctacgccctt catccagggt
     2281 ggtcactcca agatccgttc gctcgccccg aaggtttcgg ccggcatttc cgtgatgctc
     2341 ccgctgcacc gtcacttcct caagcgcggc ggcatcatgc gcaagaacac aatcgttgac
     2401 gaaatcatca aggacgaaac gggccgctgc gtcggcgtcg ccgtccgcga aaactatgtc
     2461 ttcgacatca actcccgcga caacgacctg acgaacaaga cgggcgttcg caagtactac
     2521 cgtgccgaaa agggcatcgt gttcggtgcc ggcggcttcg tcaacgactg gcgcatgtgc
     2581 tcgctcatcg acccgaagat cacgaaggaa actgaaggca cgaaccatcc gggcgccacg
     2641 gccggcgctc tcttcacgct gatgcgcgcg ggtgccattc ccgttcacat cgactacatc
     2701 cagtggggtc cctggtgctc gcctgatgac aagggcatcg gcatgggtgc cgacgtctgc
     2761 gattacatca agcagtacgg cttcgccatc gacccgaaga ccggccgccg gtgcttcaac
     2821 gagctcggca accgcactga actcacgaac gacatcttct ccctcaagaa cgaagacggc
     2881 tcccaacgct tcgccgtgct gatcgccgac agccagttcg tgccgaaagt ctccatcgac
     2941 cgttatctct ccaagtccct caagtcgggc acgacgaaga agtttgaaac gctcgaggaa
     3001 cttgccaagt tctacggtct cccgctcaag gagctcaagg acgaaatggc gaagtacaac
     3061 ggctggatca aggaaggccg tgagttcgat cctgacttca accgtccgct cacgccgaac
     3121 gaaggcgtgt tgatgggcaa ggggccctgg tacggtcacc gtctcacctc caagctccac
     3181 tacacgatgg gaggtgcgcg catcaacgaa aaggccgagg tcatcggtca ggacttcaag
     3241 ccgattccgg gcctctacgc tgccggcgaa atcacgggcg gcgtccacgg cgtcacgcgc
     3301 cttggcggca actcggtcct cgacggcatc gtcttcggcc gcatcgcagc ccagagcatc
     3361 atggccaagt aatcgtgaga aggagaagca tcatgaagaa actcgttgtc atcgccatgg
     3421 ccgctctttt cacggccggc gcctacgccg ccgatgtcgc cattccgacg tccgccaagc
     3481 acaagcagga ctgcacgatg tgccaccagg acggcttcaa gcccgtcccg acggaaaagt
     3541 gcctgacctg ccacgccgac atcaacaagg gaagctacac gctcgccaac ggcaaggaaa
     3601 acccgcacgt ttcgatccat tacctcccgg aaacggtcga ctgcaacctc tgccatcgag
     3661 agcacggcaa gtcccagaac tactgccagg cctgtcatgc cgacggtctt gggttcaagg
     3721 tgccgtaacc ctggcataag ctgtcatcga gcgtgatgga acacaaaacg cgccctgcgg
     3781 gcctttcggt ctgcagagcg ttttttgtat ggaaaattct cgaaacaaaa tacccgggag
     3841 gccacgacat caatagtgga aagcactgtg gcgctggctg actggtcggg cagaatgaac
     3901 ataaggacag ggaaaagccg gacacctcga ttcgcgacgg cgtgaacaaa tcatgcatca
     3961 cgacacgcgc gaggcgcacc gtccggccgt ttcccttcct ttccgacaca accattgtcg
     4021 gcgcagccct ttggcaactg cgctggccct tgactcgagg ttgcaacatg ctcttgacgg
     4081 acaatgaccg aaacgctctc gaagcctgcg atgagaacgg cgtcacgaat gtaggacgca
     4141 tcttcgcctt gctcgacaac ttcgtggaag agggtgtcca gcagggccga ttctcacgcg
     4201 aagaagccga tgccgatctg gaaatcgcgc tctggcgctc gttcgcgctt ctgcagtgcg
     4261 acgactacgt ttcatatgca caggcggtcg aagtgctcca aaaggccgaa gccgcggccc
     4321 accagatact tcacatcttg gatggtatgc tcgtagcgta atcccaaacg gcccgaaagt
     4381 ctcttcacct tcagtccata tcc
//
LOCUS       DBMDTI010000157         4375 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4716_length_4375_cov_3.110201, whole genome shotgun sequence.
ACCESSION   DBMDTI010000157 DBMDTI010000000
VERSION     DBMDTI010000157.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4716_length_4375_cov_3.110201"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="ACZPB6_07505"
     CDS             <1..324
                     /locus_tag="ACZPB6_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR1184348.1"
                     /translation="GRGNPPSPRERPEEARRMMERGGVVVLDVRTPEEFATGHIKGAV
                     NVPVDAIRPGMRLPFAPNPDTPVLIYCRTGRRAEYAGEALVASGYRHVYNFLGVTQWP
                     YELVR"
     gene            436..1416
                     /locus_tag="ACZPB6_07510"
     CDS             436..1416
                     /locus_tag="ACZPB6_07510"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR1184349.1"
                     /translation="MYSRDLGTGSALPKRIVTNGEFAAMLARDGIETSDEWIRTRTGI
                     ESRHFADEAEGETTSSLAVAAARRALEAANVEASEIDLIIVATTTPDMVFPSVACRVQ
                     AALGARGCAAFDVQAVCAGFIYALNAADAMLRAGPYRAAIVIGAEVMSRVLDMKDRST
                     CVLFGDGAGAVVLKASSDPGILAARMYADGDYGEKVLSLTASLNHGAISGDPYIRMDG
                     RQVFRLAIEGLVGSAREVADLACVHTEDVALFVPHQANLRIMTMVAAKLGIPEERMVR
                     TVAEHGNTSAASVPLALDRAVRAGMVKPGELVLLQGVGAGMAWGSALIRW"
     gene            1531..2460
                     /gene="fabD"
                     /locus_tag="ACZPB6_07515"
     CDS             1531..2460
                     /gene="fabD"
                     /locus_tag="ACZPB6_07515"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR1184350.1"
                     /translation="MRIAFVFPGQGSQSVGMLSGFAGNETVAGVMARADEALGFSLTG
                     LIAEGPAETLSLTVNTQPAMLASSVAFYEAWLAAGGRTPEMMAGHSLGEYSALCAAGA
                     MSLETAVRLVRFRAEAMQSAVPVGVGGMAAVIGLADDAVEAAVAEARETGRVEAVNFN
                     APGQVVIAGEKAAVARAIECAKARGCRRAIELPVSAPFHSSLMQPAADKLAARLAETK
                     IQVPAIPVIANIDVEVHETPDAIREALARQAAGAVQWVRTIERMKAAGITDIVECGPG
                     KVLTGLLRRTAPEINGHNIFDAASLAAVLEKLN"
     gene            2478..3239
                     /gene="fabG"
                     /locus_tag="ACZPB6_07520"
     CDS             2478..3239
                     /gene="fabG"
                     /locus_tag="ACZPB6_07520"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR1184351.1"
                     /translation="MTTFSANTLEGRVALVTGTSRGIGAAILEAFVKAGATVVGTATS
                     EAGAERITARIAELDGKGRGIVLNVCDGEASAKAVADTVAEFGRIDVLVNNAGITRDT
                     LVMRMKDDAWNDVVETDLTAAFRLARACVRPMMKQRSGRIISITSVVGAMGNAGQVNY
                     AAAKAGLMGMTKALAREIGSRGITVNCVAPGFIDTDMTAGLPEEHKAALLAQIPLGRL
                     GGAQEIADACLFLASDAGAYVTGTTLHVNGGMFMG"
     gene            3403..3642
                     /gene="acpP"
                     /locus_tag="ACZPB6_07525"
     CDS             3403..3642
                     /gene="acpP"
                     /locus_tag="ACZPB6_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1184352.1"
                     /translation="MEDIELKVKKIVAEQLGIKEEDIKNESSFIEDLGADSLTTVEMS
                     LALEDAFGIEIPDTEQENLRTVQQAIDYVKSAVNK"
     gene            3828..>4375
                     /locus_tag="ACZPB6_07530"
     CDS             3828..>4375
                     /locus_tag="ACZPB6_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HIR1184353.1"
                     /translation="MMNRRVVVTGLGMVSPIGNDVASSWKAALEGKSGIDRITAFDAS
                     AFGCQIAGEVKDFDAGKYLGVKEVRRFDRFVHFGVSAGIMALEDSGLEITDEFAPECG
                     CAIGSGIGGLPLICTNNEACNERGPRRISPFLIPGCIINMTSGQLAIMKNLKGPNIAH
                     VTACSTGLHAIGEAMHIIARGDA"
BASE COUNT          759 a         1532 c         1346 g          738 t
ORIGIN      
        1 ggccgcggca atccgccctc accaagagaa cgtcccgagg aggcccgcag gatgatggaa
       61 cgcggaggcg tcgtggtgct cgacgtccgc acgcccgagg agttcgccac ggggcacatc
      121 aagggcgccg tcaacgtgcc cgtcgatgcg atccgtccgg gcatgcgcct gccgttcgcg
      181 cccaatcccg acacgcccgt cctcatctac tgccgcacgg gccgcagggc cgaatatgcg
      241 ggcgaggcgc tcgtcgcctc aggctatcgc cacgtctaca acttcctcgg ggtcacgcag
      301 tggccctatg agctcgtccg ctaagtcctg cgggcgtttt gcgtcttatt acaaatgccg
      361 ggggagcgca ccgcgtgcgc tcccctttct tcgttatgat ttcggggttt tcacgaatag
      421 agaaggaaag cctccatgta ctcacgcgat ttaggcacgg gcagcgcgct tccgaagcgc
      481 atcgtcacca acggggaatt cgcggccatg ctcgcccgcg acggcattga gacgagcgac
      541 gagtggattc gcacgcgcac cggcatcgag agtcgccatt tcgccgacga ggccgagggc
      601 gagacgacgt cgtctttggc cgtggccgcc gcccgcaggg cgcttgaggc cgcgaacgtc
      661 gaggcttccg aaatcgacct gatcatcgtg gccaccacga cgcccgacat ggtctttccg
      721 tccgtcgcct gccgcgtgca ggccgcgctc ggcgccaggg gatgcgcggc cttcgacgtg
      781 caggcggtct gtgcgggctt catctatgcg ctcaacgccg ccgacgccat gctgcgcgcg
      841 ggtccctacc gcgccgcgat cgtgatcggc gccgaggtga tgtcccgagt gctcgacatg
      901 aaggaccgct ccacctgcgt gctcttcggt gacggcgcgg gcgctgtcgt gctcaaggcc
      961 tcgtccgatc ccggcattct cgccgcccgc atgtacgccg acggcgacta cggcgaaaag
     1021 gtgctgagcc tcacggcctc cctcaaccac ggcgccatct cgggcgatcc gtacatccgc
     1081 atggacggcc gccaggtctt ccgtcttgcc atcgagggcc ttgtcggcag cgcacgtgaa
     1141 gtggccgacc tcgcctgcgt gcataccgag gacgtcgcgc tcttcgtgcc gcaccaggcc
     1201 aacctcagga tcatgacgat ggtggcggcc aagctcggca ttcccgagga gaggatggtg
     1261 aggaccgttg ccgagcacgg caacacgtcg gccgcgagcg tgccgcttgc gctcgaccgc
     1321 gctgtgcgcg cgggcatggt gaagcccggc gagctcgtcc tgcttcaggg cgtcggcgcc
     1381 ggcatggcct gggggtcggc gctcatccgc tggtaagcgg ggctcaaaag cccgaaaacc
     1441 ccgccaacac gggaggggaa ttcctgtttt cggtcgatgc cccacctgct aaactcctac
     1501 cgattcaaat caacccatag gaccagttct atgcgcattg cattcgtgtt tcccggccag
     1561 ggcagccaga gcgtcggcat gctgtcgggc tttgccggca acgagaccgt cgccggcgtg
     1621 atggcccgcg ccgacgaggc gctcggcttt tcgctcaccg gcctcattgc cgaaggcccc
     1681 gccgaaacgc tttcgctcac ggtcaacacc cagcctgcga tgctcgcctc gtcggtcgcc
     1741 ttctatgaag cgtggctcgc cgccggcggc cgcacgcctg aaatgatggc gggccattcg
     1801 ctcggcgaat attcggctct ttgcgccgca ggcgcgatgt cccttgagac ggccgtgcgt
     1861 ctcgtgcgct tccgcgccga ggccatgcag agcgccgtgc ccgtcggcgt gggcggcatg
     1921 gccgccgtca tcggtcttgc cgacgacgcc gtcgaggccg ctgtcgccga ggcccgtgag
     1981 acgggccgcg tcgaggccgt caacttcaac gcgcccggtc aggtcgtgat cgcaggtgaa
     2041 aaggccgccg tcgcccgcgc catcgagtgc gccaaggccc gcggctgccg ccgcgccatc
     2101 gagctgcccg tgtccgctcc cttccactcg agcctgatgc agcccgccgc cgacaagctt
     2161 gcggcccgtc tggccgagac caagattcag gtgcccgcca ttcccgtcat cgccaacatc
     2221 gacgtcgagg tgcacgagac gcccgacgcc atccgcgagg cgctcgcccg tcaggctgcg
     2281 ggcgcggtcc agtgggtgcg cacgatcgag cgcatgaagg ccgccggcat caccgacatc
     2341 gtcgagtgcg gccccggcaa ggtcctcacg ggcctgcttc gccgcactgc gcctgaaatc
     2401 aacggccaca acatcttcga cgccgcctcg ctcgccgcgg tgctcgaaaa gctgaactga
     2461 aatttggaga aagacatatg acgacgtttt ccgccaatac gctcgagggc cgcgtcgcgc
     2521 tcgtgaccgg cacgagccga ggcatcggcg ctgccattct tgaagccttc gtgaaggccg
     2581 gcgccaccgt ggtcggcacg gccacgagcg aagccggcgc cgaacgcatc acggcccgca
     2641 tcgccgaact cgacggcaag ggccgcggca tcgtgctcaa cgtctgcgac ggcgaggcct
     2701 ccgccaaggc cgtggccgac acggtcgccg aattcggccg catcgacgtt ctcgtcaaca
     2761 acgccggcat cacccgcgac acgctcgtga tgcgcatgaa ggacgacgcc tggaacgacg
     2821 tcgtcgagac cgacctcacc gccgccttcc gtcttgcccg cgcctgcgtg cgcccgatga
     2881 tgaagcagcg ctccggccgc atcatctcca tcacgtccgt cgtgggcgcc atgggcaacg
     2941 ccggccaggt caactatgcc gccgccaagg cgggcctcat gggcatgacc aaggccctcg
     3001 cccgcgagat cggctctcgc ggcatcacgg tcaactgcgt cgctccgggc ttcatcgaca
     3061 cggacatgac ggccggtctt cccgaagagc acaaggccgc gcttctcgcc cagatcccgc
     3121 tcggccgcct cggcggcgct caggagatcg ccgacgcctg cctcttcctc gcgagcgatg
     3181 cgggcgccta cgtgacgggc acgaccctgc acgtcaacgg cggcatgttc atgggctgac
     3241 gctccctccg ccgggcatga ggcgctccgg agggagcctc ccttcacaca aagcggctct
     3301 tgcttggtaa aatattcaat cacctaaacc ccgaagggga ttgtcccttc actttttccc
     3361 cgcgcctgcg gggtcctacc ccattaaagc ggagttaaag aaatggaaga tatcgaactg
     3421 aaggttaaga agattgttgc tgaacagctc ggcatcaagg aagaggacat caagaacgag
     3481 tcctccttca tcgaagacct cggcgccgac agcctcacga cggttgaaat gtccctcgcc
     3541 ctcgaagacg ccttcggcat cgaaatcccg gacacggaac aggaaaacct ccgtaccgtc
     3601 cagcaggcca tcgactacgt caagtctgcc gtcaacaagt aatttcgcct cgagtgagct
     3661 cgatgtgaac ggcgtcctca ggggcgcccg aattgtggga tccgcctctg cgggtcccac
     3721 agttgtttta aggggaaggc ggataacgcc gacccgctag tccctaaggc cgaaaatcgt
     3781 atcagattgt tcggcttcca agaacaccca cagcaaggaa taccaccatg atgaaccgtc
     3841 gtgttgtcgt taccggtctc ggtatggttt cgccgatcgg caacgacgtc gcctcgagct
     3901 ggaaggccgc cctcgaaggc aagtccggca tcgaccgcat caccgctttc gacgcctccg
     3961 ccttcggctg ccagatcgcc ggcgaagtga aggacttcga cgcgggcaag tacctcggcg
     4021 tcaaggaagt gcgccgcttc gaccgcttcg ttcacttcgg cgtctccgcc ggcatcatgg
     4081 cgcttgaaga ctccggcctc gagatcaccg acgagttcgc gcctgaatgc ggctgcgcca
     4141 tcggttcggg catcggcggc cttccgctca tctgcacgaa caacgaggcc tgcaacgagc
     4201 gcggtccgcg ccgcatctcg cccttcctca ttccgggctg catcatcaac atgacctcgg
     4261 gccagctcgc gattatgaag aacctcaagg gcccgaacat cgcccacgtg accgcctgct
     4321 cgacgggcct tcacgccatc ggcgaggcca tgcacatcat cgcccgcggc gacgc
//
LOCUS       DBMDTI010000158         4373 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4719_length_4373_cov_3.030431, whole genome shotgun sequence.
ACCESSION   DBMDTI010000158 DBMDTI010000000
VERSION     DBMDTI010000158.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4719_length_4373_cov_3.030431"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2205
                     /locus_tag="ACZPB6_07535"
     CDS             <1..2205
                     /locus_tag="ACZPB6_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR1184354.1"
                     /translation="TRLPQLLINGSSGIAVGMATNIPPHNLGETIDACLHQLRNPECS
                     IEDLIRLMPAPDFPTGGIIFGISDVHQGYRTGRGRVVMRARTHLEEFGRDRVRIIVDE
                     IPYMVNKRVMYEKMHELMREKKIEGIAEMRDESSKEIRICIDLKQGAMPEVVLNNLFK
                     MTQMQESFGMNMVALVDGQPTLLNLQQMIVHFLSHRREVVTRRTVFRLGKYRAQGHLL
                     EGQTVALSNLDEFIRIIRNAKTPADAADELYGRGWPTAMAQELASRSVEDMHRYYPED
                     MDPVRGIQQDGLYYLTRVQIDNILAMSLRRLTGLEQDKVRADYAAMMANIADCLDILA
                     RPERVNAIIGEELENIREEYGDERRSTIDLMGDPNFNKRDLIPRRDMVVTLSRGGYIK
                     SQDLADYDAQTRGGKGRKVQEMTKDDEVAELFIANSHDVIMCFSTAGRVYPIDVFELP
                     EGKSNTRGRPIVNLLPLEEGEQISFMLPIQGFDNEHYIVMATNRGTIKKTPLSAFRNA
                     RLKGIIATTLDEGETLVGVAVSDGGDDIMLFSDAGKCARFSESVLRPMGRAAHGVIGM
                     RLDEGQSVISLIVTNDEQKYVLAATEHGYGKRTLVSEYQRKGRGIRGVITISTKERNG
                     RMVSAVLTDPDDEIMMLTTGGKVVRTSASEVSIVNRAAMGVRLINPGDDTLASVRRVA
                     VKSADDELEKLNIEVSDEDLLVDEDPADDESETPETDSDNAVDVDGDDQDQN"
     gene            2325..3413
                     /gene="serC"
                     /locus_tag="ACZPB6_07540"
     CDS             2325..3413
                     /gene="serC"
                     /locus_tag="ACZPB6_07540"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR1184355.1"
                     /translation="MGRPFNFAAGPSMLPESVLKSVSDAVIDYEGHGYSILEMMHRKP
                     PFEAILLELKSRVKSLLAVPDDFEILFCPGGGKTQFAMVPLNLVTQGCRGGYVVTGTW
                     SRMASEECRRVSRETTLWAGNNRELPANVIHVPRDVEYVYFCDNETVDGTEFKGLPAV
                     DDNDALFVSDMSSNFMSRPVDWTRYAAVWAGVQKNFGTAGLACVIVRRDQLGCAAETV
                     PQMLDWSVYARNNSLQNTPPVLQFYTAMLMARWVDEQGGVAEMDRRAKERSALLYDTI
                     DSMGDFYVSRIAPEARSRMNVVFTLADETLLPVFVKEAEAQGLTNLAGHRSVGGLRAS
                     LYNVMPMEGVERLTAFMRDFAGRHVERH"
     gene            3397..4233
                     /gene="pheA"
                     /locus_tag="ACZPB6_07545"
     CDS             3397..4233
                     /gene="pheA"
                     /locus_tag="ACZPB6_07545"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR1184356.1"
                     /translation="MSSVTDGVVAFLGPAGTFSEQAVRAVFGREAKAIAEPDFDAVLD
                     AVESGEARFGVIAIENNTNGTVTHALDLLLERRLCIVGEVSVPVVHNLLTLEKRLEDV
                     RRVYAHAQALAQCRGWLSRHMPDAERIAVSSNAEGARRASIERGAAGIAAIVAADIYG
                     LRAAAKSIQDGEGNRTRFLVMAREPEMILETPKAFKTSIVFSVPNVPGSLYDMMTPMA
                     ENGVQMVRIESRPARNGFWDYNFFIDVEGSVETPGVREALAAMEARTEHWRLLGSYPV
                     VD"
BASE COUNT          815 a         1492 c         1332 g          734 t
ORIGIN      
        1 acccgtctgc ctcagcttct catcaacggc tcctccggca tcgccgtggg catggccacg
       61 aacatcccgc cgcacaatct cggcgagacg atcgacgcct gtctgcacca gctccgcaat
      121 cccgagtgct cgatcgagga cctcattcgc ctgatgcccg ctcccgattt cccgacaggt
      181 ggcatcatct tcggcatctc cgacgtccat cagggctacc gcacgggccg cggccgcgtg
      241 gtcatgcgcg cccgcacgca tcttgaggaa ttcggccgcg accgcgtccg catcatcgtc
      301 gacgagattc cgtacatggt caacaagcgc gtcatgtacg agaagatgca cgagctcatg
      361 cgcgagaaga agatcgaggg catcgccgaa atgcgcgacg agtcctcgaa ggagatccgc
      421 atctgcatcg acctcaagca gggcgccatg cccgaggtcg tgctcaacaa cctcttcaag
      481 atgacgcaga tgcaggagag cttcggcatg aacatggtcg ccctcgtgga cggccagccg
      541 acgctgctca acctgcagca gatgatcgtg cacttcctgt cgcaccgccg cgaagttgtg
      601 acgcgccgca cggtcttccg cctcggcaag taccgcgccc agggccatct gctagaaggc
      661 cagacggttg cgctctcgaa ccttgacgag ttcatccgca tcatccgcaa cgccaaaacg
      721 ccggccgacg ccgccgacga gctctacggc cgcggctggc ccacggccat ggcccaagag
      781 cttgccagcc gcagcgttga ggacatgcac cgctactatc ccgaggacat ggaccccgtc
      841 cgcggcatcc agcaggacgg tctctactac ctcaccaggg ttcagatcga caacatcctt
      901 gcgatgagcc tgcgtcgcct gaccggtctc gagcaggaca aggtccgcgc cgactacgcc
      961 gccatgatgg ccaacatcgc cgactgcctc gacattctcg cccgtcccga acgcgtcaac
     1021 gcgatcatcg gcgaggagct cgagaacatc cgcgaggagt acggcgacga gcgccgctcg
     1081 acgatcgacc tcatgggcga tccgaacttc aacaagcgcg acctgattcc gcgccgcgac
     1141 atggtcgtca cgctctcgcg cggcggctac atcaagagcc aggacctcgc cgactacgac
     1201 gcccagacgc gcggcggcaa gggccgcaag gttcaggaga tgacgaagga tgacgaggtc
     1261 gccgagctct tcatcgccaa ctcgcacgac gtgatcatgt gcttttccac ggcgggcagg
     1321 gtctatccta tcgacgtctt cgagctgccc gagggcaagt ccaacacccg cggacgtccg
     1381 atcgtcaacc tgcttccgct cgaagagggc gagcagatct ccttcatgct gccgattcag
     1441 ggctttgaca acgagcacta catcgtgatg gccacgaacc gcggcacgat caagaagacg
     1501 ccgctctccg ccttcaggaa cgcccgcctc aagggcatca tcgccacgac gctcgacgag
     1561 ggcgaaacgc tcgtcggcgt ggccgtctcc gacggcggcg acgacatcat gctcttcagc
     1621 gatgcgggca agtgcgcccg cttctccgag agcgtcctcc gtccgatggg gcgtgcggcc
     1681 cacggggtga tcggcatgcg tctcgacgaa ggccagtccg tcatctccct cattgtcacg
     1741 aacgacgagc agaagtacgt gctcgccgcc accgagcacg gctacggcaa gcgcaccctc
     1801 gttagcgagt atcagcgcaa gggccgcggc atccgaggtg tgatcaccat ctccacgaag
     1861 gagcgcaacg gccgcatggt ctccgccgtc ctcacggatc ccgacgacga gatcatgatg
     1921 ctcaccacgg gcggcaaagt cgtgcgcacg agcgccagtg aagtcagcat cgtgaaccgc
     1981 gcagccatgg gcgtgcgtct catcaacccc ggcgacgaca cgctcgcctc ggtgcgccga
     2041 gtcgcagtca agagcgccga cgacgaactc gagaagctca acatcgaggt ctccgacgag
     2101 gacctgctcg ttgatgagga tcccgccgac gacgagtccg agactcccga gaccgattcc
     2161 gacaacgccg tcgatgtcga cggagatgat caggatcaaa actgatgcag gattctgcag
     2221 tcggcaattg ctgacgagat cgcctaagat aaacggatgc gccgcttttt tgcagcgcat
     2281 ccgttttttc atggttccgc cgtttttttt gtctggagat agtcatgggt cgtcccttca
     2341 acttcgccgc cggtccgagc atgctgcctg agtccgttct caaaagcgtt tccgacgccg
     2401 tcatcgacta cgaggggcac ggctactcca ttctcgagat gatgcaccgc aagccgccct
     2461 tcgaggcgat ccttctcgag ctcaagagcc gcgtgaagtc gcttctggcc gtgcccgacg
     2521 acttcgagat cctcttctgc ccgggcggcg gcaagacgca gttcgccatg gtgccgctca
     2581 acctcgtcac ccaggggtgc cgcggcggct acgtcgtcac gggcacctgg tcgcgcatgg
     2641 cgagcgagga gtgccgccgc gtctcccgcg agacgacgct ctgggctggc aacaaccgcg
     2701 agctgccggc gaacgtgatc catgttccgc gcgacgtcga gtacgtctac ttctgcgaca
     2761 acgaaacggt cgacggcacc gaattcaagg gcctgcccgc cgttgacgac aacgacgctc
     2821 tcttcgtctc cgacatgtcg agtaacttca tgagccgtcc cgtcgactgg acccgctatg
     2881 cggccgtctg ggccggcgtt cagaagaact tcggcaccgc gggcctcgct tgcgtgatcg
     2941 tgcgccgcga ccagctcggc tgcgccgccg aaaccgtgcc gcagatgctc gactggagcg
     3001 tctacgccag gaacaacagc ctgcagaaca cgccgcccgt gcttcagttc tacaccgcca
     3061 tgctgatggc ccgctgggtc gacgagcagg gcggcgtcgc cgagatggac cgtcgcgcga
     3121 aggagcgctc ggcccttctc tacgacacca tcgacagcat gggcgacttc tacgtgagcc
     3181 gcatcgcgcc cgaagcccgc agccgcatga acgtcgtctt cacgctcgcc gacgagacgc
     3241 tcctgcccgt cttcgtgaag gaggccgagg cgcagggcct gacgaatctc gccggccacc
     3301 gctccgtggg cggcctgcgc gcgtcgctct acaacgtgat gcccatggag ggcgtcgaga
     3361 ggctcaccgc cttcatgcgc gacttcgcgg ggcgccatgt cgagcgtcac tgacggcgtc
     3421 gtcgcctttc tcgggcctgc ggggaccttc tccgagcagg ccgtcagggc cgtcttcgga
     3481 cgtgaggcga aggcgatcgc cgaacccgac ttcgacgccg ttctcgatgc ggtcgagagc
     3541 ggtgaggcgc gtttcggcgt cattgcgatt gaaaacaaca cgaacggcac ggtcacgcac
     3601 gcgctcgact tgttgctcga gcgccgcctc tgcatcgtgg gcgaggtgtc cgtgcccgtc
     3661 gtccacaatc tgctcacgct cgagaagcgt ctcgaggacg tcaggcgtgt ctacgcgcat
     3721 gcgcaggcgc tcgcgcagtg cagggggtgg ctctcgcgtc acatgcccga cgccgagcgc
     3781 atcgccgtct cgtccaatgc cgaaggggcc cgcagggcct cgatcgagcg gggcgcggcg
     3841 gggattgccg cgatcgtggc ggccgacatc tacggcctgc gcgccgcagc caagagcatt
     3901 caggacggcg agggcaaccg cacgcgcttc ctcgtgatgg cgcgcgagcc cgagatgatc
     3961 ctcgaaactc cgaaggcctt caagacgagc atcgtcttct cggtgcccaa cgtgccgggc
     4021 tcgctctacg acatgatgac gccgatggcc gagaacggcg tgcagatggt ccgcatcgag
     4081 agccgtcccg cccgcaacgg cttctgggac tacaacttct tcatcgacgt cgagggctcg
     4141 gtcgagaccc ccggcgttcg cgaagcgctt gcggccatgg aggcgaggac cgagcactgg
     4201 cgccttctcg gatcttaccc tgttgtagac tgatggcctc gccagcgcga agagatccgc
     4261 tgggcgagcc aacagacgag agatttcatg acagactgca ttccggttat tacgcttgac
     4321 ggccccgccg cgagcggcaa gggcacgatc gcgtcccgag tcgccgacgc gct
//
LOCUS       DBMDTI010000159         4353 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4741_length_4353_cov_1.926152, whole genome shotgun sequence.
ACCESSION   DBMDTI010000159 DBMDTI010000000
VERSION     DBMDTI010000159.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4741_length_4353_cov_1.926152"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(869..2137)
                     /locus_tag="ACZPB6_07550"
     CDS             complement(869..2137)
                     /locus_tag="ACZPB6_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1184357.1"
                     /translation="MTELPPLLPEYLEPVRALFEKPRVKQAMKLLDDGAEAAMVMQCE
                     LCEIPAPTFHEEVRAAEIVRRMKALGLKDVHVDEIGNVIGRRPGRGDGPVLAIGAHMD
                     TVFPEGTPIKVRREGDRYWAPGIADNCCGLRCLLETIRAFEETGVETEGDIWFVGTVG
                     EEGNGDIRGSKHLFNGTNHIDGFLAVDNADMGRLLYAAIGSHRYRFTITGPGGHSWTN
                     FSECPSAVHAMCLAGAKVAHVKVPDGPRTTFTIGTIKGGTSVNTIAASCQVDVDMRSL
                     DDGNLAALEAMIFKCFEEGVAEENAIWGVTDPAKQVRLEVIMIGDRPGGQRPHDCPVL
                     QTSRAAMDCLGLELKRYTCSSTDANKPVSLGIPATCLSGGGAMYRTHTVDEYYDAIHI
                     EEGPKMVFLTACALAGAEGMKALLPKLPAA"
     gene            2343..3701
                     /locus_tag="ACZPB6_07555"
     CDS             2343..3701
                     /locus_tag="ACZPB6_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013045.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1184358.1"
                     /translation="MPLSSVPFVIEFPFVLGRPAPLPDPWTTAPDACVLEARRRFEAA
                     GFVPAGFTGRVGLMLVEHDGIVRAEALLEPVRAGLMTRWQDATAALVQPRVEDEDVRE
                     ALLVDGKGRGTTFREVLAEVFAPFPDRDDDHGGREQRARRAVWRGFLTDGLDDPCVRL
                     LMRRNADPARRDSSRLIALTSWWRGPSPGWDIRHDGTFCAPKSGARFLLSKLLEGIPH
                     EPPRPQQDPAGTAEPEVLYEDDFMVVVDKPARLASVPGGSETTSAREILERTRGELFV
                     VHRLDMGTSGVLAFAKTKEALRPLNLAFADRTAQKTYIARLEGLLEGEERQQGSIALP
                     IALHWFERPKQCVLPVSEGGREALTDYEVIGVEDTPEGLKSLVALHPRTGRTHQLRVH
                     CAHPEGLGLPIDGDPFYGTRGLLEETREHRLCLHAASLRITHPMTGRPLEITAPFDFP
                     TF"
     gene            complement(3782..4099)
                     /locus_tag="ACZPB6_07560"
     CDS             complement(3782..4099)
                     /locus_tag="ACZPB6_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184359.1"
                     /translation="MVTLQELQACHSPEKQARIRAFAEELLKTFEANHATYVNARKAL
                     EAAEKERRRQKALARKRARAEAAKLRAAKAKAAEKAAKAEEKTREKAKAKAAEASKEK
                     TQA"
BASE COUNT          735 a         1489 c         1329 g          800 t
ORIGIN      
        1 aaatccaccg accgaccggc agatcttgct gaattcgggg cggacccaca aaatccgttc
       61 ccgaccggac gccgcgcaac cccgcaagca agtaggcggg aagcacaagc gacaagagaa
      121 gcatacaggg aatccgcccg cccgtttgcc cccgttcact cgcgaatccg ccgcgacgag
      181 accgtgaatt gcgttttccg caattcgaag catgcgcaaa agctgccctc gctaaatttc
      241 tttcttttca ttggcacggc gcacgaccgg acgcccgagc gcgtcgtcgc gaatctctcc
      301 cagcccgcac acgagaacaa aacaattctc actccccttc gcgccgtgcg aaattcaatt
      361 gtcacatccg tgcgctccct tgagcggccc gcacaaaacc ctaaattcgc ccgaaacctc
      421 acccgtccta cccatttcgg gatatgtgcc acgcaacaac tcaaacggcc gtcacgaata
      481 tcaccccttc agcccccatc gggaatggat gaccagatga gatctcacca tacggcactt
      541 tcactatcgt acgtaaaaaa tcttaattat aaaattcagt atttctactt agcgcaatac
      601 aaaactgcgt gctcaagagt cgagcaggat cgtgcatcgt cggttcatgc aggtcctgca
      661 gatgcgcgtg gggcgggatt gcgcttaccg ctacgaagtt gtcgggaacc ccggaaggtg
      721 gcgaaagcgg gtttccgctc gttccgagag ggacttcgcc gcagctgacg cccgctctcc
      781 caaacttcgt gaaaaggggc cgcctgcgga cgttcgcggg cggccccttc agtcgtcttc
      841 gataaggaag acggaatcgg attcaggctc aggcggcagg gagcttcggg agaagcgcct
      901 tcatgccctc ggcgcccgcg agtgcgcagg ccgtgaggaa gaccatcttg gggccttcct
      961 cgatgtggat ggcgtcgtag tactcgtcga cggtgtgggt gcggtacatc gcgccgccgc
     1021 ccgagaggca ggtggcgggg atgccgaggc tcacgggctt gttggcgtcg gtcgaggagc
     1081 aggtgtagcg cttgagctcg aggccgaggc agtccatggc ggcgcggctc gtctggagga
     1141 cggggcagtc gtgcggacgc tggccgcccg gacggtcgcc gatcatgatc acttcgagcc
     1201 tgacctgctt cgcgggatcc gtgacgcccc agatggcgtt ctcctcggcc acgccttcct
     1261 cgaagcactt gaagatcatg gcttcgagcg cggcaagatt gccgtcgtcg agcgagcgca
     1321 tgtcgacgtc gacctgacag gaggcggcga tcgtgttgac gctcgtgccg cccttgatcg
     1381 tgccgatcgt gaacgtagtc ctcgggccat cgggcacctt cacgtgcgcg accttcgcgc
     1441 ccgcaaggca catggcgtgg accgcgctcg ggcattccga gaagttcgtc caggagtggc
     1501 cgcccggacc cgtgatcgtg aagcggtagc gatggctgcc gatggcggcg taaagcagac
     1561 gacccatgtc ggcgttgtcc acggcgagga agccgtcgat gtggttcgtg ccgttgaaaa
     1621 ggtgcttgga gccgcggatg tcgccgttgc cctcctcacc cacggtgccg acgaaccaga
     1681 tgtcgccttc ggtctcgacg cccgtctcct cgaaggcccg gatcgtctca agaaggcagc
     1741 gcaggccgca acagttgtcg gcgatgccgg gcgcccagta gcggtcgcct tcgcggcgga
     1801 ccttgatcgg cgtgccttcg gggaacaccg tgtccatgtg cgcgccgatg gcgagcaccg
     1861 gaccgtcgcc cctgccggga cgacggccga tcacgttgcc gatctcgtca acgtgcacgt
     1921 ccttgaggcc gagcgccttc atgcggcgca cgatctcggc ggcgcggacc tcttcatgaa
     1981 aagtcggcgc aggaatctcg cagagctcgc actgcatgac catcgcggcc tcggcgccat
     2041 cgtcgaggag cttcatcgcc tgcttgacgc gcggcttttc aaagagcgcc ctgacgggct
     2101 cgaggtactc gggaagaagc ggaggcagtt cggtcatgat gtccatcctc atgtttgaat
     2161 agaaagggat agtccgcacg ctcgcacgaa tcctttccgg cgggcgcgcg gctttgccgc
     2221 caaattctat gcccgccgcc cgtttcttcc gcacgggctt cgggaaagtc ccgatcagat
     2281 gccgctggtc taaaatgcct gcatttccgt ccgactttac gtcctccaga cgccgtccgc
     2341 ccatgccgct ttcctcagtg cccttcgtca tagaatttcc cttcgtcctc ggacgccccg
     2401 ccccgcttcc cgatccctgg accacggccc ccgacgcctg cgtcctcgag gcccgcaggc
     2461 gcttcgaggc tgcgggcttc gtccccgcgg gattcacggg acgcgtgggc ctgatgctcg
     2521 tcgaacatga cggcatcgtg cgggccgaag ccctgctcga gcccgtgcgg gccgggctca
     2581 tgacccgctg gcaggacgcg accgccgcgc tcgtgcagcc ccgcgtcgag gacgaggacg
     2641 tgcgcgaggc gctcctcgtc gacggcaagg gccgcgggac gaccttcagg gaggtgctcg
     2701 ccgaggtctt cgcgccgttt cccgaccgcg acgacgacca cggcggacgc gagcagcgcg
     2761 caaggcgcgc cgtctggcgc ggctttctca ccgacggact cgacgatccg tgcgtgcgcc
     2821 ttctgatgcg ccgcaatgcc gaccccgccc gcagggattc gagccggctc atcgcgctca
     2881 cgagctggtg gcggggcccg tctccgggct gggacatccg ccacgacggc accttctgcg
     2941 ccccgaagtc gggcgcaagg ttcctgctct cgaagctcct cgagggcatt ccccacgagc
     3001 ccccgcgtcc ccagcaggat cccgccggga cggccgagcc ggaagtcctc tacgaggacg
     3061 acttcatggt cgtggtcgac aagcccgcga ggctcgcctc ggtgcccggc ggcagcgaga
     3121 cgacgagcgc aagggagatc ctcgagcgca cccgcggcga actcttcgtc gtgcaccgcc
     3181 tcgacatggg cacctcgggc gtgctcgcct tcgccaagac gaaggaggcc ctgaggcccc
     3241 tcaacctcgc cttcgccgac cgcaccgcac agaagaccta catcgcgcgc ctcgagggcc
     3301 tgctcgaggg cgaggagcgc cagcagggct cgatcgcact cccgatcgcg cttcactggt
     3361 tcgagcgccc gaagcagtgc gtgctgcccg tctccgaagg cggccgcgag gccctcaccg
     3421 actacgaggt gatcggcgtc gaggacaccc ccgaaggcct caagagcctc gtggccctgc
     3481 acccgcgcac gggcagaacc catcagctgc gcgtgcactg cgcacacccc gaaggactcg
     3541 gccttcccat cgacggcgac cccttctacg gcacccgcgg cctccttgag gaaacccgcg
     3601 agcacaggct ctgcctgcac gccgcatcac tcaggatcac gcacccgatg acgggccgcc
     3661 cgctcgaaat caccgctccg ttcgactttc cgaccttcta aaagcaaaca gcccctgcgg
     3721 aatcccacag gggctgtttg ctttttgtct gccttcgccg cccgacctct cgggctggcg
     3781 ttcaggcttg cgtcttttcc ttcgacgcct cagcggcctt tgccttcgcc ttttccctcg
     3841 ttttttcctc ggccttcgcg gccttctccg cagctttggc cttggcggcc ctgagcttgg
     3901 cggcctcggc tctggcgcgc ttgcgtgcaa gcgccttctg gcggcggcgc tccttctcgg
     3961 cggcctcaag cgccttgcgg gcgttcacgt atgttgcatg attggcttcg aaggtcttga
     4021 gcagctcctc cgcaaaggcg cggatgcggg cctgcttttc gggcgaatga catgcctgga
     4081 gttcctgaag cgtaaccacg agttgtctcc gtttgtgtgt tggtcctgaa tgttccgttc
     4141 cgggggatcg gagccttcat gatacagaat gctgcgcccg aatggacggt cccgtccagg
     4201 ccgccccttc ccatggatct cctcccgagc gcgcacattt gaagctcccc tgtctaagac
     4261 agaggattca ccttgaccgt cagacagctc tttcgggggg caaagccgcc tgaacgtcac
     4321 cgtctgtaca aagtgtgcgc ggcagacctc ttt
//
LOCUS       DBMDTI010000160         4348 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4748_length_4348_cov_2.442665, whole genome shotgun sequence.
ACCESSION   DBMDTI010000160 DBMDTI010000000
VERSION     DBMDTI010000160.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4748_length_4348_cov_2.442665"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /gene="cysS"
                     /locus_tag="ACZPB6_07565"
     CDS             <1..1192
                     /gene="cysS"
                     /locus_tag="ACZPB6_07565"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR1184360.1"
                     /translation="DKIIRRAAERGITTTELTEEFGRAIQEDMLALGCLAPTHEPRAT
                     DYIPEMLGLIEKLEQKGYAYQGSDGDVDFAVRKFENYGRLSGKSIDDLQSGARVDVAA
                     GKRDPLDFVLWKSAKPGEPQWESKWGCGRPGWHIECSAMSAKILGDTFDIHGGGPDLI
                     FPHHENEIAQSEAASGVPFAKVWMHSGPLRVRDASGHEEKMSKSLGNFWTIRDALKDT
                     EAAYGEGNGAEVLRFFLLRSQYRSPISFSSALIEEAQKALVRLYGALKGREAAEGEVD
                     WSEKYAAQFKAAMDDDFNTPLAIAAMFELATEINRTGDPVLVRQLKGLGAVLNILQLD
                     PENFLKGGASDADEARINALIEERREAKKAKNFARADEIRKSLLDEGIVLMDGPQGTT
                     WRRI"
     gene            1228..2196
                     /locus_tag="ACZPB6_07570"
                     /pseudo
     CDS             1228..2196
                     /locus_tag="ACZPB6_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
     gene            2527..4113
                     /gene="tilS"
                     /locus_tag="ACZPB6_07575"
     CDS             2527..4113
                     /gene="tilS"
                     /locus_tag="ACZPB6_07575"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR1184361.1"
                     /translation="MSDAPRKAPARKSAPRRSKALAERLTQLVYDALSDAAETLLGVK
                     GDEFSLRPLNPVRVVVALSGGRDSMALLDVVEKLFHRPRQFLIARVHAVYVNHGLSPN
                     ADDWETHCRAECEARRIPFTPVRVTVRQTGEGVEAAAREVRYRALARFAREEAYDVVL
                     TAHHEDDRIETFLMQWMRGAGPEGLAAFPQTRELVIPGLAASRNAPPLLLVRPWSGVL
                     RADIDRYARSVKLSYVEDESNRSPKYLRNRIRREVVPLLDKIRPGFRQAAARAVSLTA
                     EAAEVLRSVSLDDLARCRDEANPHALRIPALLRLIPARQAWCLRTWMQEEGMQLPNRA
                     RLEEGLRQVRQSHADSQLALQVRSKEMRRWGDLLVIRDVSPRRSEDTRDAVLVWEGGD
                     TLALPGWNGSLAVIPCLPGECGVSARRLRDGTLEVRARRGAERMKLWPNRPSKYLKDL
                     FAEAGVPAFERADLPLVWLDGELVFTGALGMDIRFCDELAHDDTLVRFEFRSEASLLG
                     APADRPNLADRPASERRGGR"
     gene            4201..4288
                     /locus_tag="ACZPB6_07580"
     tRNA            4201..4288
                     /locus_tag="ACZPB6_07580"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4235..4237,aa:Ser,seq:tga)
BASE COUNT          744 a         1529 c         1367 g          708 t
ORIGIN      
        1 cgacaagatc atccgccgcg ctgccgagcg cggcatcacg acgaccgagc tcaccgagga
       61 gttcggccgc gcgatacagg aggacatgct cgcgctcggc tgcctcgcgc ccacgcacga
      121 gccccgcgcc accgactaca tccccgaaat gctcggcctc atcgagaagc ttgagcagaa
      181 gggctacgcc taccagggtt ccgacggtga cgtcgacttc gccgtgcgca agttcgagaa
      241 ctacggccgc ctctccggca agtcgatcga cgacctgcag tcgggcgccc gcgtcgacgt
      301 ggctgccggc aagcgcgatc cgctcgactt cgtgctctgg aagagcgcca agccgggcga
      361 gccccagtgg gaatccaagt ggggctgcgg ccgtccgggc tggcacatcg agtgctcggc
      421 catgagcgcc aagatcctcg gcgacacgtt cgacatccac ggcggcggtc ccgacctcat
      481 cttcccgcac catgagaacg agatcgccca gagcgaggcc gcctccggcg tgccgttcgc
      541 caaggtctgg atgcactcgg gtccgctgcg cgtgcgcgac gcctccggcc acgaggagaa
      601 gatgtccaag tcgctcggca acttctggac gatccgcgac gctctcaagg acaccgaagc
      661 cgcctacggc gagggcaacg gcgccgaagt gctccgcttc ttcctgctgc gcagccagta
      721 ccgcagcccg atcagcttct cgagcgcgct cattgaggag gcccagaagg ccctcgtgcg
      781 cctctacggg gccctcaagg gccgcgaggc tgccgaaggc gaggtcgact ggagcgagaa
      841 gtacgccgcc cagttcaagg ccgcgatgga cgacgacttc aacacgccgc tcgccatcgc
      901 cgcgatgttc gagctcgcca ccgagatcaa ccgcacgggc gatccggtcc tcgtgcgcca
      961 gctcaagggc ctgggcgccg tgctcaacat cctgcagctt gaccccgaga acttcctcaa
     1021 gggcggcgcc tccgatgccg acgaggcccg catcaacgcc ctgatcgagg aacgccgcga
     1081 ggccaagaag gcgaagaact tcgcccgcgc cgacgaaatc cgcaagtccc tccttgacga
     1141 gggcatcgtg ctcatggacg gcccgcaggg cacgacctgg cgccgcatct gagttccacg
     1201 gacccatttc aggacatatc gaccatcatg gcaaaacgcg tttttcttga atttgaacac
     1261 gacatcgagg ccttcgagaa gaaggtcgac gagctcaggg agcttgccga gaacacctcc
     1321 gagagcgccg tcgccgtctc gatcgacgcc gaagtcgcgc agctggagaa caagtcccgc
     1381 gagcttctga agaaggtcta cgcggacctc acgccctggc agatgtcgct cgtcgcgcgc
     1441 catccgaacc gcccgtacac gctcgactac atcgacatga tgttcacgga cttccaggag
     1501 ctccacggcg accgcgcctt tgcggacgac gaggcgatcg tgggcggtct cgcccgcctc
     1561 gccgacatga atgtcatcgt gatcggcaac cagaagggcc gcacgctgcg cgagcgcacc
     1621 cgccgcaact tcggcatggc ccgtcccgag ggctacagga aggccctgcg cctcatgaag
     1681 ctcgccgaga agttcggcct gcccgtcatc accttcgtcg ataccccggg cgcctatccc
     1741 ggcatcgacg ccgaggagcg cggccagtcc gaggccatcg gccacaacat ctacgcgatg
     1801 tccaccctca acgtgccgat cgtctcctgc gtgattggcg agggcggctc cggcggcgcg
     1861 ctcgcgatcg ccgtcgctga caccgtgatg atgcttcagt actcgaccta ctcggtcatc
     1921 agtcccgagg gctgcgcgtc cattctcttc aaggacgccg gccaggcccc gcgcgccgcc
     1981 gaggcgctcg ccctgacggc cccgcgcctc tcgcagctcg gcctcgtcga ccgcgtgctc
     2041 tccgagccgc tcggcggcgc ccaccgcgat cccaagctca tggcgaccac cctcaagaag
     2101 gctctcgtcg acgagcttcg cgtcctcatg aagcacgaca tgccgacgct gctcgcacgc
     2161 cgcgccgaga ggctcgaggg ctacggccgc ttcgagcgcc tcgctgagga ggttcccgag
     2221 cccgaggctc ccgccgccgc gcctgaagct caacccgacg ccaaggccga tccctgcgat
     2281 tgctcgtgcg ccaagaaggc cgcacaggcc gcgaagtccg tgaaggccag gcctgccgcc
     2341 gaagaagcga aggccgctga agcggaaaag gccgaataag cggacctcac cgtcccgccc
     2401 gtctcaaaaa ggcacgtttc tccatggagg acgtgccttt tttggatttc ttcccgttcg
     2461 tgtcgcccgc cgcagtacaa tgacggcttt gtttttcacg gccctttgag tccctccagt
     2521 ccgcccatgt ccgacgctcc cagaaaagcc ccagcccgca agtccgcgcc gcgccgctcg
     2581 aaggcgctcg ccgagcgtct gacgcagctc gtctacgacg cgctctccga tgcggccgaa
     2641 acgcttctcg gcgtcaaggg ggacgaattc tcgctcaggc cgctcaatcc cgtgcgcgtc
     2701 gtcgtggcgc tttcgggcgg gcgcgactcc atggcgctcc ttgacgtcgt cgagaagctc
     2761 tttcatcgtc cgcgccagtt tctcatcgcc cgcgtgcatg cggtctacgt caatcacggc
     2821 ctctcgccca atgcggacga ctgggagacg cactgtcgcg ccgagtgcga ggcaaggcgc
     2881 attccgttca cgcccgtgcg cgtgacggtc aggcagacgg gcgaaggcgt cgaggccgcg
     2941 gcccgcgagg tccgctacag ggcgcttgcg cgctttgcac gcgaggaggc ctatgacgtc
     3001 gtgctgacgg cgcatcacga ggacgaccgc atcgagacct tcctcatgca gtggatgcgc
     3061 ggcgcggggc ccgaaggact cgccgccttt ccgcagacgc gcgagctcgt gatccctgga
     3121 cttgcggcct cgagaaacgc gccgccgctt ctgctcgtgc gcccctggag cggcgtgctt
     3181 cgcgccgaca tcgacaggta cgcccgaagc gtgaagctca gctacgtcga ggacgagtcg
     3241 aaccggagtc cgaagtacct gcgcaaccgc atccgccgcg aggtcgtgcc gcttctcgac
     3301 aagatccgtc cgggcttcag gcaggccgcg gcgcgcgccg tttcgctcac ggccgaggcg
     3361 gccgaggtgc tccgctccgt ttcgctcgac gatcttgcgc ggtgcaggga cgaggcgaat
     3421 ccgcacgccc tgcgcattcc cgctcttctc aggctgatcc ccgcccgcca ggcctggtgc
     3481 ctgcgcacct ggatgcagga ggaaggcatg cagctgccca accgagcgcg tcttgaggag
     3541 gggctcaggc aggtgaggca gagccacgcc gactcgcagc ttgcgcttca ggtgcgctcc
     3601 aaggagatgc gcaggtgggg cgaccttctc gtcatccgcg acgtctcgcc cagacggtcg
     3661 gaggatacgc gcgatgcggt gctcgtctgg gaaggcggcg acacgctggc gcttcccggc
     3721 tggaacggct ctctggccgt cattccgtgc ctgcccggag agtgcggcgt gagcgcgagg
     3781 cgcctgcgcg atggcaccct cgaggttcgc gcccgccgcg gcgccgaaag aatgaagctc
     3841 tggccgaacc gtccgtcgaa gtacctcaag gacctcttcg ccgaggcggg cgtgcccgcc
     3901 ttcgaacgcg ccgaccttcc gctcgtctgg ctcgacgggg agctcgtctt cacgggcgcg
     3961 ctcggcatgg acatccgctt ctgcgacgaa ctcgcccacg acgacacgct cgtgcgcttc
     4021 gaattcaggt ccgaagcgag tctgctcgga gcgcccgcgg acaggcccaa tctcgccgac
     4081 aggcccgctt cggagcgtcg aggcgggcgc tgagaacgtt ttttccgctt ttgccggaac
     4141 aagcgatgcc tgtggacttt ttttcattac ttctgtagaa tgcgaaactt atctgacgtc
     4201 ggaagtgtgg cagagtggtt gaatgcactg gtcttgaaaa ccagcgaggg gcaacccttc
     4261 gtgggttcga atcccaccgc ttccgccaat ggagctatac ctgttcgact gaggggcggt
     4321 cctactactg tgcgtttggg ccgcatgt
//
LOCUS       DBMDTI010000161         4333 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4757_length_4333_cov_2.032609, whole genome shotgun sequence.
ACCESSION   DBMDTI010000161 DBMDTI010000000
VERSION     DBMDTI010000161.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4757_length_4333_cov_2.032609"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(228..1016)
                     /locus_tag="ACZPB6_07585"
     CDS             complement(228..1016)
                     /locus_tag="ACZPB6_07585"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR1184362.1"
                     /translation="MKDLEHEASGNVFPGDLRERVSRQMILRELGEKGQAAISSAHFL
                     VIGAGGLGSPAILYLAAAGAGTITVVDADTVSVSNLSRQILHTTSTVNVNKAKSAAIA
                     VERLNPHCRIIPVSEFADAVKLKALVKNADVILDCTDNLATRIAVSRAAHDAGKPLVF
                     ASAVRFSGQLTVFDPSVPESPCFECIFEEDDPGNDVKAAAVGVFSSVTGIVGTLQATE
                     ALKIAAGIGSTLCGRMLFIDALNMQFDTIRVKRRKRCPVCGERH"
     gene            complement(1048..1974)
                     /gene="cysK"
                     /locus_tag="ACZPB6_07590"
     CDS             complement(1048..1974)
                     /gene="cysK"
                     /locus_tag="ACZPB6_07590"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR1184363.1"
                     /translation="MIHQSSLELVGGTPLLQLNNWMRLHGLKANVIAKIEALNPAGSV
                     KDRAARQMILEAEKAGLIGPGAVIIEPTSGNTGIGLASIAAARGYRAIFTMPETMSVE
                     RRMLLKAYGAEVVLTPGTAGMKGAIEKAEELAREIPGSFIPQQFENPANPLAHYLTTG
                     PEIWADTDGHVDMFVAGVGTSGTVTGNGRYLKEKNPAVKVVAVEPDASPVLSGGKAGP
                     HAIQGIGANFVPGNYDASVVDEVFRVPNDAAIAAARALPKAEGILIGISGGAALHAAT
                     ELAKRPENEGRNIVVIFPDNGERYLSTVLYAE"
     gene            complement(2313..2756)
                     /locus_tag="ACZPB6_07595"
     CDS             complement(2313..2756)
                     /locus_tag="ACZPB6_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024988.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1184364.1"
                     /translation="MKENTRIPFLAGRLSEATSTFILESLKREGMGELLPCHGDILHV
                     LTEQETATVMELSRLTHRSKSTVSALVTKLVDLGYVARERSAEDSRVINLRLTEKGRA
                     FIPVSKRISSELDALVTRSLTPDEARFAEALLARILNGFSSDKSA"
     gene            complement(2879..3724)
                     /locus_tag="ACZPB6_07600"
     CDS             complement(2879..3724)
                     /locus_tag="ACZPB6_07600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR1184365.1"
                     /translation="MNTRISALAAALLIALAAPAMSADVPQGTIKGKAWVQTDRTMAE
                     AMAQSWTVLPASVTGDKTYEGTNIRAPETKGKAPAVIFVHGSGGINPQIKAFQKRLAD
                     ELGIASFTADSFQLPDRMTYTSPIDPMDYEKIHALRASELTAAVEFLAKQPWFNGRFV
                     IAGTSEGAVAVARYVRAEAAPAEAGRIIFSWSCENNYHVVQHKTRIPDGMPVLNIMSS
                     KDKYFSQANPYLGNHEAVGNCSKALQGNPNARIVLIKDAPHTLMNEPEAREAEDKFLK
                     DVLLK"
     gene            complement(3993..>4333)
                     /locus_tag="ACZPB6_07605"
     CDS             complement(3993..>4333)
                     /locus_tag="ACZPB6_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1184366.1"
                     /translation="NLKGSIDTYNAVVRGEKKDPLGFVANNKADKVMTEGPWYACQKV
                     PTVHHTMGGLQINADAQVLDVNGKVIPGLYAAGETTGGIHGSNRLGGNAIADCMVFGR
                     TAGTNAAQGK"
BASE COUNT          692 a         1261 c         1493 g          887 t
ORIGIN      
        1 gccggcacgt cctccatcgg cggcaaggtc ggcgtcaagt acgccttctg aaactgagat
       61 gatgcgcctg cggggcgaat gctccgcagc ctgacgtcaa aggcagggcg cccggtacct
      121 tcaggaggag ccggacgccc ttttctttgg atcgtttccg acagaacgac cccggacttt
      181 cgtctcgggg ccgttccttt tgtttggctt gagcgctctc ttctctttca gtgcctttcg
      241 ccgcagacgg ggcagcgctt cctgcgcttc accctgatgg tgtcgaactg catgttgagc
      301 gcgtcgatga agagcatcct gccgcagagc gtcgagccga tgcctgcggc gatcttcagg
      361 gcctcggtgg cctgaagcgt ccccacgatg cccgtgaccg acgagaagac gccgacggct
      421 gcggccttga cgtcgttgcc cggatcgtcc tcctcgaaga tgcactcgaa gcaaggagat
      481 tcgggcacgg aaggatcgaa gacggtgagc tggcccgaaa agcgcacggc ggacgcaaag
      541 acgaggggct tccctgcatc atgcgcggcc ctcgagaccg cgatgcgggt tgcgaggttg
      601 tccgtgcagt cgaggatcac gtccgcattc ttcacgaggg ccttgagctt cacagcgtct
      661 gcgaattcgg acacggggat gatgcggcaa tggggattga gcctctcgac cgcgatggcg
      721 gcgctcttgg ccttgttgac gttgaccgtc gacgtcgtgt gaaggatctg gcgcgagagg
      781 ttcgagaccg acaccgtgtc ggcgtcgacc accgtgatcg tgcccgcgcc tgcagcggcg
      841 aggtagagga tggcgggaga gccaaggccc cctgctccga tcacaagaaa gtgcgcgctg
      901 ctgatggcgg cctgcccttt ctctccgagc tcgcgcagaa tcatctggcg gctcacgcgc
      961 tccctgaggt ctccgggaaa gacgttcccg ctggcttcgt gctcaagatc cttcatgatt
     1021 tttgtgactc cgtgaggctg atgcgtctta ttcggcgtag agaaccgtgg agagatagcg
     1081 ctcgccgttg tcggggaaga tcacgacgat gttcctgcct tcgttttcgg ggcgcttcgc
     1141 gagttccgtc gcggcatgga gggcggcgcc gcccgatatg ccgatgagga tgccttcggc
     1201 tttcgggagg gcgcgcgcag ctgcgatcgc ggcgtcgttc ggcacgcgga agacctcgtc
     1261 gaccaccgag gcgtcgtagt tgcccggcac gaagttcgcg ccgatgccct ggatggcgtg
     1321 cggaccggcc ttgccgcccg agaggacggg actcgcatca ggctcgaccg ccaccacctt
     1381 gacggcggga ttcttctcct tgagatagcg gccgttgccc gtcaccgtgc ccgacgtgcc
     1441 cacgcccgcg acaaacatgt cgacatgtcc gtccgtgtcg gcccagatct ccggaccggt
     1501 cgtgaggtag tgggcgagcg ggttggcggg gttttcgaac tgctgcggga tgaagctgcc
     1561 gggaatttcg cgggcgagtt cctcggcctt ttcaatcgcg cccttcatgc cggccgtgcc
     1621 gggcgtaagg acgacttcgg cgccgtaggc cttgagcagc atgcggcgct cgacgctcat
     1681 cgtctcgggc atcgtgaaga tggcgcgata gccgcgggcg gcggcaatgc tcgcgagccc
     1741 gatgcccgtg ttgcccgagg tgggctcgat gatgacggcg cccggaccga tgaggccggc
     1801 cttttccgct tcgaggatca tctggcgcgc ggcgcggtcc ttgacgctcc cggcgggatt
     1861 gagggcctcg atcttggcga tgacgttcgc cttgaggccg tggagcctca tccagttgtt
     1921 gagctgcaga agcggcgtgc cgccgacaag ttcaaggctc gactgatgaa tcatgatgtt
     1981 tctcctgtgc gtgtttggag ttgtcgttgt gctgcgggat ctccgtccgg cgtccgaagg
     2041 tgtcttcccg acggatgccc gcctgctcca ctctcccggg aagaacgccg tccggcgccc
     2101 gagaacaccc gtcctgcagg cgttcgcata attcctgagt gagttggtag gaaattggat
     2161 cctactccat cacacgctgt tttgcaatgg tttgcgtctc tgcgcccgaa gcagatcaaa
     2221 gcgcggaggt cgtccgtcga tcttagcaaa gatgagggcc gggctgtggt tgagatcagg
     2281 gcggaaagct cgtcggaagt gtccggaggg cctcaggcgg acttgtcgga ggaaaagccg
     2341 ttgaggattc tcgcgaggag cgcttcggcg aatcgggcct cgtcgggcgt gagcgagcgc
     2401 gtcacgaggg cgtcgagttc ggaggagatg cgtttggaca cggggatgaa cgccctgccc
     2461 ttttcggtaa gcctcaggtt gatgacacgt gagtcctcgg ccgagcgctc gcgcgcgacg
     2521 tagcccagat ctacgagctt cgtgacgagg gctgaaacgg tggacttcga gcggtgcgtg
     2581 aggcgtgaga gctccatcac cgtggcggtt tcctgttcgg tcagcacgtg caggatgtcg
     2641 ccgtggcacg ggagcagttc gcccatgcct tcgcgcttga gcgactcgag aatgaatgtt
     2701 gaggtggcct ccgagaggcg gcctgcaaga aaggggatgc gggtgttttc cttcatgagg
     2761 cgatgttagt tcgagggcga acgaaagtca acgaaccgca ggacgaaaag gcccgctgga
     2821 ggcatgaagc ctgcggcggg ccgtgcgcat gacggattgg gtggaatccg ggcgtcgact
     2881 acttgaggag gacgtccttg aggaacttgt cctcggcctc gcgagcctca ggctcgttca
     2941 tgagcgtgtg cggcgcgtcc ttgatgagga cgatgcgggc gttgggattg ccctggagcg
     3001 ccttgctgca gttgccgacg gcctcgtggt tgccgagata gggattggcc tgcgagaagt
     3061 acttgtcctt cgagctcatg atgttgagga cgggcatgcc gtcgggaatt ctcgtcttgt
     3121 gctggacgac gtggtagttg ttttcgcacg accaggagaa gatgatgcgg cccgcttcgg
     3181 cgggtgcggc ttcggcgcgc acgtagcggg ccacggcgac tgcgccttcg ctcgtgcctg
     3241 cgatcacgaa cctgccgttg aaccagggct gtttcgccag gaattcgacg gccgccgtga
     3301 gttcgctcgc acgaagcgcg tggatcttct catagtccat cgggtcgatg ggcgaggtgt
     3361 aggtcatgcg gtcggggagc tggaaggagt cggccgtgaa gcttgcgatg ccgagctcgt
     3421 ccgcgagacg cttctggaag gccttgatct gcggattgat gccgcccgag ccgtgcacga
     3481 aaatcacggc gggcgccttt cccttcgttt cgggcgcgcg gatgttggtg ccttcgtatg
     3541 tcttgtcgcc cgtcacggat gcgggcagta ccgtccagga ctgcgccatg gcctcggcca
     3601 tcgtgcggtc ggtctgcacc caggccttcc ccttgatcgt gccctgcggc acgtcggcgg
     3661 acatggcggg cgcggcgagc gcaatgagaa gcgcggcggc aagtgcggaa atccttgtgt
     3721 tcatggtctc tctctccttg atgaacgttc gtgcgcaccg ggactcgggt cgattcaccc
     3781 gtcgtgctgc aggcgcaccc tgccaagaat agtcgtattg cccgcattcg ggcgagcgcc
     3841 gaaaaactct gaaacaacga gcggccatgg gtcgtcgtgg tgccgtgtcg tcatgctcaa
     3901 caacgataaa ggccctcctt cagacgggct cccggatgga cgggagcccg aaggagagcc
     3961 ttccggcatg ctcaggcctg cgccgtcacg gattatttgc cctgagcggc gttcgtgccg
     4021 gcggttcggc cgaagaccat gcagtcggcg atggcgttgc cgccgaggcg gttggagccg
     4081 tggatgccgc ccgtcgtttc accggcggcg tagaggcccg gaatcacctt gccgttgacg
     4141 tcaagcacct gtgcgtcggc gttgatctgc aggccgccca tcgtgtggtg gacggtcggg
     4201 accttctggc aggcgtacca ggggccttcg gtcatgacct tgtcggcctt gttgttggcg
     4261 acgaagccga gcggatcctt cttttcaccg cgtacgacgg cgttgtaggt gtcgatcgaa
     4321 cccttgaggt ttt
//
LOCUS       DBMDTI010000162         4325 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4769_length_4325_cov_1.830019, whole genome shotgun sequence.
ACCESSION   DBMDTI010000162 DBMDTI010000000
VERSION     DBMDTI010000162.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4769_length_4325_cov_1.830019"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(175..747)
                     /locus_tag="ACZPB6_07610"
     CDS             complement(175..747)
                     /locus_tag="ACZPB6_07610"
                     /inference="COORDINATES: protein motif:HMM:NF015325.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1184367.1"
                     /translation="MKILLINGSPREGNTARVLEALAARLGGHDVETLRLGELGIRGC
                     IGCLRCQQDVEHFVCHQTGDDANAVLAKIVAADVVLMATPLYGHCFTAQMKCLDDRMS
                     ALMKWVGGEHPHVVSALKDKPMGLAVTCGGPSAGNADLLLALFDKTAEGRQARVFGKY
                     VFEFCPGDPAGFTPDEALLARMAADVAALG"
     gene            complement(916..1431)
                     /locus_tag="ACZPB6_07615"
     CDS             complement(916..1431)
                     /locus_tag="ACZPB6_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1184368.1"
                     /translation="MTEKPLIIISSRTGNTLAVGHAVCDALPGAVMMRPSEVPDDLTP
                     YNPVLLGFWCDRGDAPEEILRIAPRLRNKTIGCFATMGGDPEGEKSKAWMQKTSEKLV
                     AMGEGNTLAETFLCRGRIDPEVFQRMTDMLGGTVSPEREAGRRLSETHPDRLDLDRAA
                     QIFGGFFGGSW"
     gene            complement(1504..2370)
                     /gene="dnaQ"
                     /locus_tag="ACZPB6_07620"
     CDS             complement(1504..2370)
                     /gene="dnaQ"
                     /locus_tag="ACZPB6_07620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HIR1184369.1"
                     /translation="MRNTQMASTTEYEDERVNLSKDAPKQARPRKKILVPPKPHEKRY
                     SSFNKPQITLDTETTGLDPDKGDRILEIGCVRLNGREISLDPDDYLQIYINPERDIPE
                     ETTAIHGITNDKVKDCPVFADIVDEFLDFVSDTELLIHNAKFDVGFINMELERLGRGR
                     LEDYVAQITDTLEIAREMYPGRRVSLDGLCLMHKIDNSARVFHGALLDSQLLAEVYLT
                     MTRGQGEIDLDNWGDAVEIPPEIIARLTMPEPSPEELAEHEKMLDKADKQCKATCAWR
                     KALGIGVEEKAS"
     gene            2744..>4325
                     /locus_tag="ACZPB6_07625"
     CDS             2744..>4325
                     /locus_tag="ACZPB6_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HIR1184370.1"
                     /translation="MSTKSFIISLPIITGDQDRRRLRKSFSFGSLMKAFRLLSAALLV
                     PLGAALAAPSATIEPDAPLRSEARTVVRIGLLDFESEASYLSRRENIQETIVRHLQEN
                     SPGLRIVPRFYTTEALSEAVRKGEVEFFLGSSGFFVQMRPMGARDIGTITSRAFPDPN
                     RCVAGVIVVRSGRADITDLARLEGMTAVTTSRANFMTYMLNMGEIAAAGFKPDKFFSA
                     VTETDNRPVEVLRAVVEGRADAGLLRACMPEAITERHPEFAGRWTVVNDKPGLRAESL
                     GCRYSTDMYPGWTIASVPHTPPVITKHVAMLLLNLKPDMTPGGFTVSIATEFDRVNDL
                     FRSLKTGPFAYLNHWTLERIWDEARGGVLLLLGFLAAWILHFARLEKLVRRRTADLSA
                     ALEREKAAREQGRETGARLDSLQRAGIVGQLSSMFAHELCQPLSAVRCYLRGMRTLMR
                     RGAPDPVLAERCLSGMSAELDKAGAIIDRVRSYAREGARRDLPVDLPALLSECLADMT
                     KSDRLKVPVAADIRPGLIV"
BASE COUNT          736 a         1430 c         1339 g          820 t
ORIGIN      
        1 cggagctgca cttcgggcaa ggcggaagag aggtcatggc ggtgtccttg aactgttgtt
       61 tctgttgggt ttctgagttg cgggcgcctg cacgcggagc gcaggcgcga aaggagcgca
      121 ttctaacatg atgcgcatcg agttccgccg aaaacgggcg ggtatttgca agactcagcc
      181 cagcgcggcg acatcggccg ccatgcgcgc gaggagcgcc tcgtcgggcg tgaagcccgc
      241 aggatcgcct ggacagaatt cgaagacgta cttgccgaac acgcgggcct gacggccttc
      301 ggcggttttg tcaaagagcg cgaggagcag gtcggcgttt cctgcggacg gtcctccgca
      361 ggtgaccgca aggcccatcg gcttgtcctt gagcgccgag acgacgtgcg gatgctcgcc
      421 gcccacccac ttcatcaggg ccgacatgcg gtcgtcgagg cacttcatct gggccgtgaa
      481 gcaatgcccg tagagcggcg tcgccatgag cacgacgtcg gccgccacga tcttcgcgag
      541 caccgcattg gcgtcgtcgc ccgtctgatg acagacgaag tgctccacat cctgctggca
      601 gcgcaggcag ccgatgcaac ccctgatgcc gagttcgccg agcctgagcg tctcgacatc
      661 atggccgccg agcctggcgg cgagggcctc aagcacgcgg gcggtgttgc cttcgcgcgg
      721 actgccgttg atgagaagga tcttcatccg tgttgtctcc tgttgttgcc cgaggggcgg
      781 gctttgggtt ccgagaggtc ctcatgatag cgcgacacgg caaggcgcac gcaaaatgcg
      841 agaaaaggcc gtgctgatga ggcacggccc tttcgatttt ctttgatccc gttcgatcgc
      901 gtcttgcgga cgcttctacc agctgccgcc gaagaagccg ccgaagatct gcgcggccct
      961 gtcgaggtcg agcctgtcgg ggtgggtttc gctcagtctg cggccggcct cgcgctcggg
     1021 actcacggtg ccgccgagca tgtcggtcat gcgctggaag acctcggggt cgatgcggcc
     1081 gcggcagagg aaggtctccg caagcgtatt gccctcgccc atcgcgacga gcttctcgct
     1141 cgtcttctgc atccaggcct tcgacttctc gccctcgggg tcgccgccca tggtggcgaa
     1201 gcagccgatc gtcttgttcc tgaggcgggg cgcgatcctg aggatctcct cgggcgcatc
     1261 gcccctgtcg caccagaagc cgagcaggac gggattgtag ggcgtgaggt cgtcgggaac
     1321 ctcggagggg cgcatcatga cggcgccggg cagcgcgtcg cagacggcat gtcccacggc
     1381 cagcgtgttg ccggtgcggg agctgatgat gatcaggggc ttctcggtca tgtcgggatt
     1441 cgttcaggaa gctggaaaaa gaaagtatag gacgccgcgc ccgtttcgcg caggcggcga
     1501 agcctacgac gccttctcct cgacgccgat gccgagcgcc ttgcgccatg cgcaggtcgc
     1561 cttgcactgc ttgtcggcct tgtcgagcat cttctcgtgc tcggcgagct cctcgggcga
     1621 gggctcgggc atggtcaggc gggcgatgat ctcgggcgga atctcgaccg cgtcgcccca
     1681 gttgtcgagg tcgatctcgc cctggccgcg ggtcatcgtc agatacactt cggcaagaag
     1741 ctggctgtcg agcagtgcgc cgtggaacac gcgggcggag ttgtcgatct tgtgcatcag
     1801 gcacaggccg tcaagactca cgcggcggcc gggatacatc tcgcgggcga tctcaagcgt
     1861 gtccgtgatc tgcgcgacgt agtcctcgag cctgcccctg ccgagacgct cgagctccat
     1921 gttgatgaag cccacgtcga acttcgcgtt gtgaataagc agctccgtgt cggagacgaa
     1981 gtcgaggaac tcgtccacga tgtccgcaaa gacgggacag tccttcacct tatcgttcgt
     2041 gatgccgtga atggccgtcg tctcctcggg aatgtcgcgc tctggattga tgtagatctg
     2101 cagatagtcg tcgggatcaa gcgagatctc gcggccgttg agcctcacgc agccgatctc
     2161 gagaatgcgg tcgcccttgt cgggatcaag acccgtcgtt tccgtatcga gcgtgatctg
     2221 cggcttgttg aagctcgagt agcgcttctc atgcggcttg ggcggcacga ggatcttctt
     2281 gcggggacga gcctgtttcg gggcgtcctt cgagaggttg accctctcgt cttcatactc
     2341 ggtggtagag gccatctgcg tgtttctcat gtgtctgttc aactgaccta actatatcgc
     2401 acattgatcc cggcgacgtc cctgcacgac cgctgctatg cgaccgcctc gactccacag
     2461 gcgcatcatc ctgcctgagc cccggcctca ccgtcctctg aaacacgtcg gcgaattcga
     2521 catgctcgtc ttctgcgtcg cgtaaaattc ctgcagcaca accaatcgtt ccgggctgcg
     2581 tccgaggctc gtcctgaacg ggatttcgcc ttcacagaac cagccgcagg aagcctccga
     2641 attcattcgg atggaggaat gcggcaaacc tctctcttcc aatctatttg ttgtccccga
     2701 tgaaaggcgt gtgtctttcc aactcctgtg ccaaggtact cccatgagca cgaaatcctt
     2761 catcatcagt cttccgatca tcaccggaga tcaagaccgc cgtcggctgc gcaagagctt
     2821 cagcttcggc tccctcatga aagcctttcg cctcctttct gcagcactcc tcgttccgct
     2881 cggcgcggcc cttgcagcgc cgtccgccac gatcgagccc gacgccccgc tccgcagcga
     2941 ggcccgcacc gtcgtgcgca tcggcctgct tgatttcgag tcggaggcat cctacctgag
     3001 cagacgcgaa aacattcagg agacgatcgt ccgccatctg caggaaaatt cacccggtct
     3061 ccggatcgtt ccgcgcttct acacgaccga agcgctctcc gaagcggtga gaaaaggcga
     3121 ggtcgagttc tttctcggca gctccggctt cttcgtgcag atgcgtccga tgggcgcgcg
     3181 cgacatcggc accatcacgt ccagggcctt tcccgatccg aaccgctgcg ttgcgggcgt
     3241 gatcgtcgtc aggagcgggc gagccgacat tacggacctc gccagactcg aaggcatgac
     3301 ggccgtcacg acgagccgcg cgaacttcat gacatacatg ctcaacatgg gcgagatcgc
     3361 cgccgcaggc ttcaagcccg acaaattctt cagcgccgtc accgaaaccg acaaccgtcc
     3421 cgtcgaggtc ctgcgagcgg tcgtcgaggg ccgagccgac gcaggccttc ttcgcgcctg
     3481 catgcccgag gccatcacgg agcgccatcc cgagttcgcg ggccgctgga ccgtcgtcaa
     3541 cgacaagccc ggccttcgcg ccgagtcgct cggctgccgc tactcgaccg acatgtatcc
     3601 gggatggacg atcgcgagcg tgccgcacac gccgcccgtc atcacgaagc atgtcgcaat
     3661 gctccttctc aacctcaagc ccgacatgac gccgggcggc ttcaccgtct cgatcgcgac
     3721 tgagttcgac cgcgtcaacg acctcttccg ctcgctcaag accggtccct tcgcctatct
     3781 caaccattgg acgctcgaac gcatctggga cgaggccaga ggcggcgtcc tcctgctcct
     3841 cgggtttctc gccgcctgga tcctgcactt cgcaagactc gagaagctcg tcaggcgccg
     3901 caccgccgac ctcagcgccg cactcgagcg cgaaaaggcc gcccgcgaac aggggcgcga
     3961 gacgggcgcg aggctcgaca gcctgcagcg cgcgggcatt gtcgggcagc tgagctcgat
     4021 gttcgcgcac gaactctgcc agccgctctc ggccgttcgc tgctacctgc gcggcatgcg
     4081 cacgctcatg cgccgcggcg cacccgaccc ggtgctcgcc gaacgctgcc tctcgggcat
     4141 gtcggccgag ctcgacaaag cgggcgcgat catcgaccgc gtgagaagct acgccagaga
     4201 gggcgccagg cgcgacctcc ccgtcgacct gcccgcactc ctctccgagt gcctcgccga
     4261 catgacgaag tccgaccgcc tcaaggtgcc cgtcgccgcg gacatccgcc ccggcctcat
     4321 cgtct
//
LOCUS       DBMDTI010000163         4319 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4773_length_4319_cov_3.449739, whole genome shotgun sequence.
ACCESSION   DBMDTI010000163 DBMDTI010000000
VERSION     DBMDTI010000163.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4773_length_4319_cov_3.449739"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(8..1366)
                     /gene="mog"
                     /locus_tag="ACZPB6_07630"
     CDS             complement(8..1366)
                     /gene="mog"
                     /locus_tag="ACZPB6_07630"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HIR1184371.1"
                     /translation="MKPPRKSENELILGLVSVSDRASRGVYDDRGIPELEAWCRKAII
                     NPMAVHKRLIPDERFEIEKTLRELVDIIGCDLVLTTGGTGPSRRDVTPEATLAVSTRE
                     MPGFGEQMRAISGHFVPTAILSRQTAVLRETPDHAALIINLPGQPKAIAETLEGLRGK
                     DGKSVVNGIFAAVPYCIDLIGGPYVETREEVVRAFRPKSAQRPKPAEEKQAEPVLPSQ
                     PAKPAEPAGKPFDPKDILMVSPRRAQNAPEAAVIWLHGMGVDNNDFAPFPDEILDFGG
                     PVCRFVLPNAPVREISAHPGYPLRAWYDVRSDKIDDNEDRAGIRETAARISLLITDVE
                     KAGIPRSRIFLGGFSQGAAAALYAGLREEEPVAGIVALSGYLPLAATLFSEITPAGRK
                     TPIFMAHGLFDSVISPLTAKRSAQVIQEINPDFRWLPFDMDHEICQDELAALAQFLRQ
                     HL"
     gene            complement(1391..1951)
                     /gene="yjgA"
                     /locus_tag="ACZPB6_07635"
     CDS             complement(1391..1951)
                     /gene="yjgA"
                     /locus_tag="ACZPB6_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HIR1184372.1"
                     /translation="MRQEQIYEDDVDENGYRGPSKSQLKREQNALQDLGREMTKLGDE
                     QLKRIGLPDEVLVEIVEFRRMRSFGAQRRQLQLIGKKMRNMDPVAVREAIDRATGESR
                     AAVALLHRCETLRDQMLAGDEALTKFISEHPEADIPRLRTLVRTARKEREAQKPPKSA
                     RELYRYLHDLLEEPISLFAEDDENEE"
     gene            2059..3399
                     /locus_tag="ACZPB6_07640"
     CDS             2059..3399
                     /locus_tag="ACZPB6_07640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR1184373.1"
                     /translation="MQDEVEMRTEEPARWTVERSKSLGADCASAEVSHDKGLSVTVRM
                     GEVDAVEHTEERVLTLNAWIGGRKGCASTADFSERSLEAAVQAAVDIARVSAEDPAAG
                     LPEAEDLQADMRDLSLWHPFEGGVQEAVRLASAVEEAVFEADERIVNSDGTNFSTSSG
                     EFTLANSLGFCAGYRYSRHDMDAAPIAEDDSGMQRDGWWTQGRAVEDLLDPAELGRTA
                     AGRTVARLGARPLSSRRARVLFDAPVATGLLDILEELLNGRAWYRHASCLEGRLGERI
                     LPAHVSVEEDPYLMRALGSGVFDDEGCAGRRRMIVSDGRIEGIFLSSYSARRLGMRTT
                     GNLGGAYNLRLSSTETRPEDTRRAMLERLGTGFLVTELIGQGLNAVSGDYSRGASGFW
                     VENGRIVAPVEGVTIASNILTMMKTIEAIGADEHDTGVRRSGSLLIPDLMLAGE"
     gene            3529..>4319
                     /gene="queA"
                     /locus_tag="ACZPB6_07645"
     CDS             3529..>4319
                     /gene="queA"
                     /locus_tag="ACZPB6_07645"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR1184374.1"
                     /translation="MQEQHLSDFDFELPPELIAQYPLENRTASRLLHITKDVIEDCTF
                     TDIEGFLHEGDLLVANNTKVIKARLLGRKATGGNVEALIERVTGERTAISMVRASKSP
                     KPGTVLYFEADGIEEPVMVTGRQGQFFELEFERPVLDVLEAFGRVPLPPYITHAAQKS
                     DESRYQTVYARYPGAVAAPTAGLHFTEDLLGRLAAKGVDIEYVTLHVGAGTFQPVRVE
                     NLSEHQMHSEWFNMPEDVAARINRAKAEGRRVIAVGTTSLRTIESA"
BASE COUNT          752 a         1259 c         1492 g          816 t
ORIGIN      
        1 tcctgtctca gagatgctgg cggaggaact gtgccagcgc cgcgagctcg tcctgacaga
       61 tttcgtggtc catgtcgaac ggaagccagc ggaagtcggg attgatctcc tgaatgacct
      121 gagcgctgcg cttggcggtc agcggcgaaa tgacggagtc gaagagaccg tgcgccatga
      181 agatgggcgt cttgcggccg gcgggcgtga tttctgagaa cagagtggcc gcgagcggca
      241 ggtagcccga aagggccacg atgcccgcga caggttcttc ttcacgaagc cccgcgtaga
      301 gtgcggcggc cgcgccctga gagaagcctc cgaggaagat gcggctgcgg gggatgcccg
      361 ccttttcgac gtccgtgatg agaagactga tgcgggctgc ggtttccctg atgcctgcac
      421 ggtcctcgtt gtcgtcgatc ttgtccgagc gcacgtcgta ccaggcgcgg agcggatagc
      481 ccggatgcgc ggagatttca cgcacggggg cgttgggcag aacgaatcgg cagacgggac
      541 cgccgaagtc aaggatctcg tcagggaagg gcgcgaagtc gttgttgtcg acgcccatgc
      601 cgtggagcca gatgacggcg gcttcaggcg cattctgcgc gcgccgcggg gagaccatca
      661 ggatgtcctt cggatcgaag ggcttgccgg cgggttccgc aggcttggcg ggttgggagg
      721 gcagaaccgg ttcggcctgc ttctcctctg cgggcttcgg gcgctgagcg ctcttggggc
      781 ggaaggccct gacgacttcc tcgcgggtct cgacgtaggg accgccgatg aggtcgatgc
      841 agtagggcac ggctgcaaag atgccgttga cgacggactt gccgtccttg cccctgaggc
      901 cctcaagcgt ttcagcaatg gcctttggct ggccgggaag attgatgatg agcgctgcat
      961 gatcgggcgt ttcgcgaagc acggccgtct ggcgcgacag aatggcggtc gggacgaagt
     1021 ggcctgagat cgctcgcatc tgttcgccga agcccggcat ctcgcgcgta ctgacggcaa
     1081 gcgtcgcttc gggcgtgacg tcgcggcgcg aggggcccgt tccgcccgtc gtgaggacga
     1141 ggtcgcagcc gatgatgtcg acgagctcgc gcaacgtttt ctcgatctcg aagcgctcgt
     1201 cggggatgag gcgcttgtgc acggccatcg gattgatgat ggccttcctg caccaggctt
     1261 cgagctcggg aatgccgcgg tcgtcataga cgcccctgct ggcgcggtca gacacggaca
     1321 cgaggcccag aatcagttcg ttttcacttt tgcgaggcgg cttcatcgaa atcgtccttt
     1381 ggcctgatgg ttattcttcg ttctcgtcgt cttcggcgaa gaggctgatg ggctcttcga
     1441 gaagatcatg aaggtagcgg tagagctcgc gggcgctctt gggcggcttc tgtgcctcgc
     1501 gctccttgcg agctgtacgc acaagcgtgc gaaggcgtgg gatgtcagct tcgggatgct
     1561 cgctgatgaa tttggtgagc gcttcgtcgc ctgcaagcat ttggtcgcgc agcgtctcgc
     1621 agcggtgaag cagggcgacg gcggcgcggg attcgcctgt ggcgcggtcg atcgcttcgc
     1681 gaacggcgac gggatccatg ttgcgcatct tcttgccgat gagctggagc tggcgtcgct
     1741 gcgcgccgaa gcttctcatg cggcggaatt cgacgatttc cacgagaacc tcgtcgggaa
     1801 ggccgatgcg cttgagctgc tcgtcgccga gcttcgtcat ctcgcgtccg agatcctgga
     1861 gcgcgttctg ctcgcgcttg agctgggatt tggaggggcc gcggtagccg ttttcgtcaa
     1921 cgtcgtcttc gtagatctgt tcctggcgca tggcaaagct cttgcgttaa aaagtcatca
     1981 aaacccttat gatagcggga tcgcgggcag gacgtcggat gtccgaaggc ccgtgaaggc
     2041 ggaggaagga gagtgaaaat gcaggatgaa gtcgaaatgc gcacggagga gcccgcccga
     2101 tggacggtgg agcgttcgaa gtcgctgggg gcggactgcg caagcgccga ggtgagccac
     2161 gacaaggggc tgagcgtgac ggtgcgcatg ggggaggtcg acgccgtaga gcacaccgag
     2221 gagcgtgtcc tgacgctcaa cgcgtggatc ggagggcgaa aaggctgcgc aagcacggcg
     2281 gacttttccg aacgctcgct cgaggcggcc gtgcaggccg ccgtcgacat cgcccgcgtc
     2341 tcggcggagg accctgctgc gggacttccc gaggcggagg atctgcaggc cgacatgcgt
     2401 gacctgtcgc tctggcatcc gttcgaggga ggcgtccagg aggccgtaag gctggcctcg
     2461 gccgtagagg aggccgtctt cgaggcggac gagcggatcg tcaactccga tggcacgaac
     2521 ttctcgacct cgtcggggga gttcacgctg gccaattcgc tcgggttctg cgctggctac
     2581 aggtattcgc gtcacgacat ggacgctgcg cccattgccg aggacgactc cggcatgcag
     2641 cgcgacggct ggtggacgca ggggcgcgcc gtcgaggatc ttctggaccc cgccgagctc
     2701 ggacgcacgg ctgccgggcg cacggtcgct cgccttggcg caaggccgct gtcgagccgt
     2761 cgtgcgcggg tgctctttga tgcgcccgtg gcgacgggac ttctggacat tcttgaagaa
     2821 ctcctcaacg ggcgcgcctg gtacaggcat gcgagctgtc tcgagggaag gcttggcgag
     2881 cggattcttc ctgctcatgt ctccgtggag gaggatccct atctcatgag ggcgctcggt
     2941 tccggagtct tcgacgacga agggtgtgcg ggacgaagac gcatgatcgt gtcggacgga
     3001 cggattgagg ggatcttcct ctccagctac tccgcccgaa ggctcggaat gaggacgacg
     3061 ggaaatctcg gcggagccta caacctgagg ctttccagca ccgagactcg acctgaggac
     3121 acgaggcggg cgatgctcga gcgccttggc acgggattcc tcgtgacgga gctgatcggg
     3181 caggggctca acgcggtctc gggagactat tcgaggggag cctcgggctt ctgggtcgag
     3241 aacgggcgga tcgtcgcccc cgtcgaaggg gtgaccattg cctcgaacat tctcacgatg
     3301 atgaagacga tcgaggcgat cggagccgac gaacacgaca cgggcgtgcg tcgatcggga
     3361 agcctgctga ttcccgatct gatgctcgcg ggcgaatgag ctctgacgaa tgagcctcga
     3421 aggggcttct tcgcaacgag gtgcaaagtc aggcgtcttt gtcgagacag tcgcgggcaa
     3481 agcgggtaga atgccgcttt tctcaaattc ccactggcct gtcaagccat gcaagaacag
     3541 cacttatccg atttcgattt cgagctcccg ccggagctca ttgcccagta tccccttgaa
     3601 aaccgcactg catctcgtct tctgcacatc acgaaggacg tgatcgagga ctgcacgttc
     3661 acggacatcg agggctttct tcacgaaggc gacctgcttg tcgccaacaa caccaaggtc
     3721 atcaaggccc gcctgctcgg ccgcaaggcc acgggcggca acgtcgaggc cctgattgag
     3781 cgcgtcacgg gcgagcgcac cgctatctcc atggttcgcg ccagcaagtc cccgaagccg
     3841 ggcactgtcc tctacttcga ggccgacggc atcgaggagc ccgtcatggt gacgggccgt
     3901 cagggccagt tcttcgaact tgagttcgag cgtcccgtac tcgacgttct cgaggcgttc
     3961 ggtcgcgttc cgcttcctcc ctacatcacg catgcggcgc agaagtccga cgagagccgc
     4021 tatcagacgg tctatgcccg ctatcccggc gccgtggccg ctccaacggc cggccttcac
     4081 ttcaccgagg atcttctcgg caggcttgcg gccaaggggg tcgacatcga gtatgtcacg
     4141 cttcacgtcg gcgcggggac ctttcagccc gttcgcgtcg agaatctttc tgaacaccag
     4201 atgcattcgg aatggttcaa catgcccgag gacgtggccg cgcgcatcaa tcgtgcgaag
     4261 gccgagggcc gccgcgtgat cgccgtcggc acgacgtcgc tgcgcacgat cgagagcgc
//
LOCUS       DBMDTI010000164         4307 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4792_length_4307_cov_2.662149, whole genome shotgun sequence.
ACCESSION   DBMDTI010000164 DBMDTI010000000
VERSION     DBMDTI010000164.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4792_length_4307_cov_2.662149"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /gene="rlmD"
                     /locus_tag="ACZPB6_07650"
     CDS             <1..1408
                     /gene="rlmD"
                     /locus_tag="ACZPB6_07650"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1184375.1"
                     /translation="SPPMTTRHLTITDIASTGAGIAYEDGLRVFVPGALPGDEVTAEV
                     DPPARGTRSSVAQYVKITRKSPWWAASPCPAHVARPACGGCALGSLTPEGQAVVKRGL
                     LERALAESGVEAPEPLPLVEAPHDAEAFTQGFRNKAVLYPGVDPEGRWYFAMYAQGSH
                     FPVPESCFCPQTPEWMAKAAETAARMLSLSDLSPWNESHREGDVRTIVMRDGVDSEKP
                     QRLFTLCVHGETPEVRIFVGKLAKKLMEEGITSVFLNLHPTPGNAVLGRHSLHVAGTD
                     GIETTIGGLRFAVRPETFLQVNPGQTERLYAMALEWVAPEKDEVLLDLYCGVGTMTLL
                     AARTCAKAVGVDIVAASIERAKLNAKRNGIENAVFHAGAVEDELPRLIASGIRPAAAI
                     LDPAFKGLEETVPAMLTALPLTRFVYVSCNPKTFARDAAKFIELGWRIERLAPVDLFP
                     GALHVETVAKFVRDAQPR"
     gene            complement(1396..3531)
                     /locus_tag="ACZPB6_07655"
     CDS             complement(1396..3531)
                     /locus_tag="ACZPB6_07655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR1184376.1"
                     /translation="MAAAEINPRLWDASTPLVERVALAFDEKGPLTKAVKGFCVREGQ
                     REFAVEAARAVEEKTILVAEAGTGTGKTFAYLTPALLAGATCVISTAGKSLQDQLCAK
                     DLPALRDALGVPVKVALLKGRANYVCHFRLELTASEGRLPEQDSYLKLRKIQRFAAVS
                     RTGDRAELPDVPEDDRLWPLVTSTRENCLGKDHCPNYDDCFVKKAREDAMQSQVVVVN
                     HHLYLSSMALKRESDAIDGMLPQAALTVIDEAHQLPGIASSFFGTSFSTYDVENVSME
                     ARRLGRTKCNDGAEWEILYDRVLKAGREFRLDAQRIGLAEGERLDVDEIEDFGELYPG
                     FERLRAAFAAMGEAMRANEGRDNELDTLAERHAELMEQMEAWTAIFVKCRNGAADEAS
                     EGEAAGDAEVGGEAGPEAGAASGADAEVRWLEVSQHGIRFNLTPLSFAEEFREMRERE
                     GGAWVFTSATLSSAGRFDLFKQRLGIGECVERTWESPFNYWEQGCFYLPQMPPPANNT
                     AVHTHNVIEKVWPLINAAGGRTFVLCTSLAAVRAAADELQARLEANGNPYPLFVQGDG
                     PKMRLIEEFRAHGNAVLVGSMSFWEGVDVKGEALSLVVIDKIPFAPPNDPVMMARSRA
                     VEASGRRPFDEITLPEAVITLKQGAGRLIRSEGDRGMLVICDPRILNKGYGKVVRDSL
                     PDFYCTRREEKALEFFLNPERFREGLYRG"
     gene            complement(3546..>4307)
                     /locus_tag="ACZPB6_07660"
     CDS             complement(3546..>4307)
                     /locus_tag="ACZPB6_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184377.1"
                     /translation="TVEDAKAANEAGSARQKEIDGALDVMKDWCHTRFLVNACIKDAR
                     DLHHEREKEIRSVRLKADEMIRLDRVEQRRARQESQKQNVKQPMKLGGSAESPESRSE
                     SRAEEVKAKQSRAEERRALEEANVRAYEEKQARAARKAEERHDPIRVKSRVKAPSSPI
                     EGHLGRTAADVEAGRAEAAERMAQEDANIAAFNAKQAEAKKRLEEAEATAAERKASRE
                     ARQENFNKTLEERRAAQKRYEESRENKDSGLKKYF"
BASE COUNT          664 a         1504 c         1351 g          788 t
ORIGIN      
        1 ttctcctccg atgacgacca ggcatctcac catcaccgac attgcctcga cgggcgcagg
       61 catcgcctac gaggacggac tccgcgtctt cgtgccgggg gcgctccccg gcgacgaggt
      121 caccgcggag gtcgatccgc ccgcacgcgg cacgcgatcg agcgtcgccc agtacgtgaa
      181 gatcacccgg aagagcccct ggtgggccgc ctccccgtgt cccgcccatg tggccaggcc
      241 cgcctgcggc ggctgcgcgc tcggcagcct cactccagag ggccaggccg tcgtcaagcg
      301 cggactgctc gaaagggcgc tcgccgaatc gggcgtcgaa gcccccgagc ccctgcccct
      361 cgtcgaggcg ccgcacgacg ccgaggcgtt cacgcagggc tttcgcaaca aggccgtgct
      421 ctatccgggc gtcgatcccg agggacgctg gtacttcgcg atgtatgctc agggctcgca
      481 ctttcccgtg cccgaatcct gcttctgccc gcagacgccc gagtggatgg ccaaggccgc
      541 cgaaaccgcg gcccgcatgc tctcgctctc ggacctctct ccctggaacg aatcgcaccg
      601 cgagggcgac gtgcgcacga tcgtcatgag ggacggcgtg gattcagaga agccgcagcg
      661 cctcttcacg ctctgcgtgc acggcgagac gcccgaagtc aggatctttg tcgggaagct
      721 tgcgaagaag ctcatggagg aaggcatcac gagcgtcttc ctcaacctgc acccgacgcc
      781 cggcaatgcg gtgctcggac gccacagcct gcacgttgcg ggcacggacg gcatcgaaac
      841 gacgatcggg gggctgcgct ttgcggtgcg tcccgaaacc ttcctgcagg tcaatccggg
      901 acagacggag aggctctacg ccatggcgct tgagtgggtt gcgcccgaaa aggacgaggt
      961 cctgctcgac ctctactgcg gcgtgggcac gatgacgctc cttgccgccc gcacctgcgc
     1021 gaaggccgtg ggcgtcgaca tcgtcgccgc ctcgatcgag cgcgccaaac tcaacgccaa
     1081 gcgaaacggc atcgaaaacg ccgtcttcca cgcgggtgcg gtcgaggacg agctgccccg
     1141 tctcatcgcc tcgggcatca ggcccgccgc ggccattctc gaccccgcct tcaaggggct
     1201 cgaggagacg gtgcccgcca tgctgacggc cctgccgctc acgcgattcg tctacgtctc
     1261 ctgcaacccg aagaccttcg cgcgcgatgc ggcaaagttc atcgagctcg gctggcgcat
     1321 cgagaggctc gcgcccgtgg acctcttccc gggcgcactc cacgtcgaga cggttgcgaa
     1381 gttcgtccgc gacgctcagc ctcggtaaag accttctctg aagcgctcgg gattgaggaa
     1441 gaactctagc gccttctcct ccctgcgcgt gcagtagaag tcgggcaggc tgtcgcgcac
     1501 gaccttgccg tagcccttgt tgaggatgcg agggtcgcag atgacgagca tgcccctgtc
     1561 gccttccgag cggatgaggc gtcccgcacc ctgcttgagc gtgatgaccg cctcgggcag
     1621 cgtgatctcg tcgaagggac gcctgcccga agcctcgacg gcgcgacttc gcgccatcat
     1681 cacgggatcg ttgggcggcg cgaagggaat cttgtcgatc acgacgagcg aaagcgcctc
     1741 acccttgacg tcgaccccct cccagaagct catcgagccc acaagcacgg cgttgccgtg
     1801 cgccctgaac tcttcgatga ggcgcatttt gggaccgtcg ccctgaacga agagcggata
     1861 gggattcccg ttggcctcga gcctcgcctg aagctcgtcc gccgcggccc tcacggccgc
     1921 gagcgacgtg cagagaacga acgtgcgtcc gcccgccgcg ttgatgagcg gccagacctt
     1981 ttcaatgacg ttgtgcgtgt gcaccgccgt gttgttcgcg ggcggcggca tctgcggcag
     2041 atagaagcac ccctgttccc agtagttgaa ggggctctcc cacgtgcgct cgacgcattc
     2101 gccgatgccg agcctctgct tgaagaggtc gaacctgccc gcgctcgaaa gcgtggccga
     2161 cgtgaagacc caggcgccgc cctcgcgctc tcgcatctcg cgaaattcct ccgcaaagga
     2221 aagcggcgtc aggttgaagc ggatgccgtg ctggctcacc tcgagccagc gcacctctgc
     2281 gtccgctccg gaagccgcac ccgcttcggg ccctgcttct cccccaacct cggcatctcc
     2341 ggcggcttca ccctcggaag cctcatcggc cgcaccgttg cggcacttca cgaagatggc
     2401 cgtccaagcc tccatctgtt ccatgagctc ggcatgacgc tcggcgagcg tgtcgagctc
     2461 attgtcgcgg ccctcattgg ccctcatcgc ctcgcccatg gccgcaaagg ccgcgcgcag
     2521 gcgctcgaag ccaggataaa gctcgccgaa atcctcgatc tcgtcgacat cgagcctctc
     2581 gccctcggca agaccgatgc gctgggcgtc gagcctgaac tcgcgccccg ccttgagcac
     2641 gcggtcgtag aggatctccc actcggcgcc gtcgttgcac ttcgtgcggc cgagcctgcg
     2701 ggcctccatc gagacgttct cgacgtcata ggtcgagaag ctcgtgccga agaagctcga
     2761 ggcgatgccg ggcagctggt gcgcctcgtc gatcaccgtg agtgccgcct gcggcagcat
     2821 gccgtcgatc gcatcggact cgcgcttgag ggccatcgag ctcagataga gatggtggtt
     2881 caccacgacg acctgactct gcatggcgtc ctcgcgggcc ttcttgacga agcagtcgtc
     2941 gtagttcgga cagtggtcct tgccgaggca gttttcgcgc gtgctcgtca cgaggggcca
     3001 gaggcggtcg tcctcgggca cgtcggggag ttccgctcgg tcgcccgtgc ggctgaccgc
     3061 cgcaaagcgc tggatcttcc tgagcttcag ataggagtcc tgctcgggga gcctgccttc
     3121 ggaggccgtg agttcgaggc ggaagtggca gacgtagttg gcgcgtccct tgagaagcgc
     3181 gaccttgacg ggcacgccga gcgcatcgcg cagggcggga aggtccttgg cgcagagctg
     3241 gtcctgaagc gacttgcctg ccgtcgagat gacgcaggtc gcgcctgcaa gaagcgcggg
     3301 cgtcagatag gcgaaggtct tgcccgtgcc cgtccccgcc tcggcgacga gaatggtctt
     3361 ctcctcgacc gcgcgggccg cctcgacggc gaactcgcgc tgcccctcgc gcacgcagaa
     3421 gcccttgacg gccttcgtga gcgggccctt ttcatcaaag gcgagtgcaa ctcgctcgac
     3481 aagcggcgtc gaggcgtccc agagacgcgg atttatttca gcagcagcca tctctttcct
     3541 tcgactcaga aatacttctt gaggccggag tccttgttct ccctggactc ctcatagcgc
     3601 ttctgtgcgg cgcgacgctc ctcgagcgtc ttgttgaagt tctcctgccg cgcctcgcgg
     3661 ctcgccttgc gttcggcggc ggtggcctcg gcctcctcaa ggcgcttctt cgcctcggcc
     3721 tgcttggcat tgaaggccgc aatgttggcg tcctcctgag ccatgcgctc ggccgcctcg
     3781 gccctgcccg cctcaacgtc agccgccgta cggccgagat gtccttcaat gggacttgac
     3841 ggagccttca cgcggctctt caccctgatg ggatcgtggc gctcctccgc ctttctggcg
     3901 gccctcgcct gcttttcctc gtaggcacgg acgttcgcct cctcaagggc cctgcgctcc
     3961 tcggcgcggc tctgcttggc cttcacctcc tcggcgcgcg actcgctgcg gctttcggga
     4021 gactcggcgg agccgccgag cttcatgggc tgcttcacgt tctgcttctg gctttcctga
     4081 cgagccctgc gctgctcgac gcggtcgagg cggatcatct cgtcggcctt gaggcgcacg
     4141 cttcggatct ccttctcgcg ctcgtgatgc aggtccctcg cgtccttgat gcaggcgttg
     4201 acgagaaagc gcgtgtggca ccagtccttc atgacgtcga gcgcaccgtc gatctccttc
     4261 tgcctggccg aacccgcctc attggcggcc tttgcatcct caaccgt
//
LOCUS       DBMDTI010000165         4294 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4809_length_4294_cov_1.964703, whole genome shotgun sequence.
ACCESSION   DBMDTI010000165 DBMDTI010000000
VERSION     DBMDTI010000165.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4809_length_4294_cov_1.964703"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            286..594
                     /locus_tag="ACZPB6_07665"
     CDS             286..594
                     /locus_tag="ACZPB6_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184378.1"
                     /translation="MKDRIGTALWVAGAIICAWALSVSLSMMFDFTIEEARQNFRQGS
                     FIFGAAVVFAFVFRSKVQTWGAFEKFLIYALVPVAGILLTAYGWCQQFAPELVASLGA
                     "
     gene            1190..1489
                     /locus_tag="ACZPB6_07670"
     CDS             1190..1489
                     /locus_tag="ACZPB6_07670"
                     /inference="COORDINATES: protein motif:HMM:NF044550.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing
                     transcriptional regulator"
                     /protein_id="HIR1184379.1"
                     /translation="MIRMNSHKTVLMPKSLEDNQVQMDNICKAFNNGSASDAAASIGQ
                     LFRCRGMSQVSMASGIGRAVLYRSFSKSGNPTLQVLLDSLRAVGLTLVVAEAKDR"
     gene            1811..2650
                     /locus_tag="ACZPB6_07675"
     CDS             1811..2650
                     /locus_tag="ACZPB6_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR1184380.1"
                     /translation="MKILIPIDASECSKNAAFWATGKPALFGKDPEIHIFYAQEPASD
                     RIRFATDPDTLDEFYREEAKTVFDSIIDRCGTLPASVTLDYGVGSPVQTIVSAGDKLS
                     ADMVVMGTHGKGPLDSLLFGSVSNDVVAHSHRPILLIRERMPTSESLKRIAICLDESD
                     QSLRVQGFFTEHVDLFEQGTSFTLIHVQKSAEDQQANGFAETGTIANEVESILTRAGM
                     NVSTKTLYGDVGDVIAEHAEEENYDMIVMGSHGYSSVSATLLGSVTMTVASECDVPLL
                     IVR"
     gene            complement(2910..4016)
                     /locus_tag="ACZPB6_07680"
     CDS             complement(2910..4016)
                     /locus_tag="ACZPB6_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR1184381.1"
                     /translation="MFKKSLAALAVLGAAAGYASAADVTLFGVVDTGLIYTHQTFADD
                     SKADVNKFSMDSGVSSASRFGLKGTEELGNGLKVGFKLENGFQSDSGALKSEKRIFDR
                     EASVSVYSDFGTLSMGRMGGVGSGAGTYDLVLATADAFDGGDNNVFGFATSDRYDNMV
                     TYQTPKFAGLQATVQYSFNEDSVEETAREGSSAVNRYSSAALTGDFGALQTVLAYEFQ
                     NYKSFGAAEHDDGQIVYLGGNYDCGFAKTFVMGQYFKGIKAGQLTAITEDDADRVDSA
                     SSDGVKGYGVHLGTIVPVAGGDLTVAAYFTDFSVEYATGDKDAKSYAFGAKYEYYLSK
                     RTSVYTGAGFAQVKYDDEGSDKTTQAYLGLTHRF"
BASE COUNT          894 a         1233 c         1294 g          873 t
ORIGIN      
        1 agcgctcccg tgaagacgtc gagcacgcgg gcgttgagaa tggcaaggtc atggatctta
       61 ggcttcgggc tctgcttggc gtcctctacg agaatttttg gatgcagtca ttctaaggcg
      121 aatgtcctct gcaaaagagg aaaacgtgca tcggcgagcc ttcgtcacgg acgaggcggc
      181 gggcgagggc ggcgcggcaa tcggatcggc ttcaacggcg ctcgttccat gggactacaa
      241 tacggaatcc ttttgcaacc acaagaaccc gactgacatc caattatgaa agacagaatc
      301 ggcactgctc tctgggtggc cggcgcgatc atctgcgcct gggccctatc ggtctccctc
      361 tcgatgatgt tcgacttcac gatcgaggag gcccgccaga acttccgcca gggctccttc
      421 attttcggcg cagccgtggt cttcgccttc gtcttccgct cgaaggtcca gacctggggc
      481 gcgttcgaga agttcctcat ctacgcgctc gtgcccgttg caggcattct gctcaccgcc
      541 tacggctggt gccagcagtt cgcgcccgaa ctcgtcgcaa gcctcggagc ctgatccggt
      601 ctccagcccg ttcacgactc ccaaaaaggc cgtttcgccg tcatcgcgaa gcggcctttt
      661 tgccggccga aaaaccgtgt tttgatatag aaatttttat ccttatagga ctgtttagtc
      721 tggtaagaac ccacaagtcc tcctgagaac tggagagggc gggcaaatgc aaccaaatcc
      781 ttcagattgt tcgccttttc acgacaatcg ggtcgaaaca ccgctcctgc agaagtcttc
      841 aggactcgag aatcagtctc atgacgcttg ggagaaacct aaagacgaca accaaacgcc
      901 gtgctcaatc atcggcgaaa aggttaattt tgggaaaaac ccttgatcag gcgcaagaaa
      961 gtttttcttt atgggcccga ctcaggtgtt tgtcgtagac agcagggggc ggcgcgactg
     1021 tatcatatac gcgaatttaa acacggcctg acggtccctc ggattcgaga ctgtcgcgcc
     1081 tgtcggggat acgcacaata cccctcaaag gcaggaacga cagggccttg cgtcaaggag
     1141 ccgccgctga aggccgaatc tcaactcgaa aatatacaga gagaaactga tgatcagaat
     1201 gaattcgcac aagacggtcc tcatgcccaa gtccctcgag gataaccagg ttcagatgga
     1261 caacatctgc aaggccttca acaatggatc cgcctccgat gccgctgcga gcatcggcca
     1321 gctcttccgc tgccgcggca tgagccaggt ttccatggcg agcggcatcg gccgcgcggt
     1381 cctctacagg tccttcagca agtccggcaa tccgacgctt caggtgctgc tcgactcgtt
     1441 gcgtgcagtc ggtctcacgc tcgttgtggc ggaagccaaa gacaggtaaa aaagacccgg
     1501 tccaaacaat caggagccgt cggaacgaac gcttccggcg gctcttttcc tatgcgtgtc
     1561 tcgtttcctg catgaccttt ggagggattt tgccgtgtaa agacggcccg tgaaggaggg
     1621 cggggtcaag gggcgctcac tcgtgacgca aaagtgctat caaatagggg gtatatgtcg
     1681 atttttcctc ccgaaggtcc gattctttct ataagataag gggcacaagg gtgttattcg
     1741 acgcctccct ttcgttccga ccgttccgac ggaccctgcg tcgaggagca cctctgagga
     1801 ggaccaaaaa atgaagattc ttattcccat cgacgcgtcc gaatgcagca agaacgccgc
     1861 cttctgggcg acgggcaagc ccgctctctt cggcaaggat cccgagatcc acattttcta
     1921 cgcgcaggag cccgcttcgg accgcatccg ctttgcgacg gaccctgaca cccttgacga
     1981 gttctaccgc gaggaggcga agaccgtctt cgactccatc attgaccgct gcggcacgct
     2041 tcccgccagc gtgacgctcg actacggcgt cggctctccc gtccagacca ttgtttccgc
     2101 cggcgacaag ctttcggccg acatggtcgt gatgggcacg cacggcaagg gcccgcttga
     2161 cagcctgctc ttcggctccg tctcgaacga cgtcgtggcg cactctcatc gtcccattct
     2221 gctcatccgc gaacgcatgc cgacttccga gtccctgaag cgcatcgcga tctgccttga
     2281 tgagtccgac cagtcgcttc gcgtccaggg cttcttcacg gagcatgtcg acctcttcga
     2341 gcagggcacg tccttcacgc tgatccacgt tcagaagagc gctgaggacc aacaggccaa
     2401 cggctttgcc gaaacgggca cgatcgccaa cgaggtcgag tccattctca cgcgcgccgg
     2461 catgaacgtc tcgaccaaga cgctctacgg cgacgtgggt gacgtcatcg ccgaacacgc
     2521 cgaggaagag aactacgaca tgatcgttat gggctcccac ggctacagtt ccgtctccgc
     2581 cacgctgctc ggctccgtca cgatgacggt tgcgagcgag tgcgacgtgc cgctcctgat
     2641 cgtccgctga caagtggatt ccctctcttg agggcaccag acgacaagac cgtcctgcat
     2701 cacgcagggc ggtctttctt ttgggcgggt tctttgtcgt agtgttgcga ggtccggaaa
     2761 tcgcgaggct tgtcgatgat gagagggaag ggtaaggtga acgaaaacgg cgggcgtgaa
     2821 aaaggccggc ggctcctttc ggaggccgcc ggcccttgct tcatgaggac tcagtgctga
     2881 ggatcatttc cgagcactga aggatccggt tagaagcggt gcgtcagacc gaggtaggcc
     2941 tgggtcgtct tgtcagagcc ttcatcgtca tacttgacct gggcgaagcc ggcacccgtg
     3001 tagacggacg tgcgcttgga gaggtagtac tcgtacttgg caccgaaggc gtagctcttg
     3061 gcatccttgt cgccggtagc atattcgacg gagaagtccg tgaagtaggc tgcaaccgtc
     3121 aggtcgccgc cggcaaccgg gacgatcgtg ccgaggtgaa cgccgtagcc cttgacgccg
     3181 tcggagctag cgctgtcgac gcgatcggca tcgtcttcag taatggcggt gagctgaccg
     3241 gccttgatgc ccttgaagta ctggcccatg acgaaggtct tggcgaagcc gcagtcgtag
     3301 ttgccgccga ggtagacgat ctggccgtcg tcatgttcag cagcaccgaa ggacttgtag
     3361 ttctggaatt cgtaggcgag gaccgtctgg agagcgccga agtcgccggt aagagcggcg
     3421 gagctgtagc ggttcacggc ggaggagcct tcacgagccg tttcctcgac ggagtcttcg
     3481 ttgaaggagt actggaccgt agcctggaga ccggcgaact tcggggtctg gtacgtgacc
     3541 atgttgtcgt agcggtcgga cgtggcgaag ccgaagacgt tgttgtcgcc gccgtcgaag
     3601 gcatcagccg tggcgaggac gaggtcatac gtgccggcac cggagccaac gccgcccatg
     3661 cggcccatgg agagcgtacc aaagtcggag tagacggaga cggaagcttc gcggtcgaag
     3721 atgcgctttt cggacttgag tgcgccggaa tcggactgga agccgttttc gagcttgaag
     3781 ccgaccttga ggccgttgcc gagttcttcc gtgcccttca ggccgaagcg ggaggcggag
     3841 gagacgccgg agtccatgga gaacttgttg acgtcagcct tggagtcgtc agcaaaggtc
     3901 tggtgcgtgt agatgaggcc cgtgtcgacg acgccgaaga gggtcacatc agcagcggag
     3961 gcatagccgg cggcagcgcc gagaacggcg agcgcggcga gagacttctt gaacatttgt
     4021 gtatttctcc aatttcctcc gtttccggag gctcaatggg tttgaattga tcgcaacgca
     4081 aatctagtgc cgatgcgcgc ggtacgcaaa cccatctcaa cgacgtccgg gcaaatgaag
     4141 gcgaacgtcc acgagcgacg cagggcattt cggaaatacc tcgacggatc cggacgaaat
     4201 gagcgctccc cttgatgcgc aagggtttgc cgccttcttt tcgcgaagga agggtattcc
     4261 cgagggacgg caaaaggggc cttcaaagag ggac
//
LOCUS       DBMDTI010000166         4292 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4812_length_4292_cov_2.512050, whole genome shotgun sequence.
ACCESSION   DBMDTI010000166 DBMDTI010000000
VERSION     DBMDTI010000166.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4812_length_4292_cov_2.512050"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="ACZPB6_07685"
     CDS             complement(<1..1406)
                     /locus_tag="ACZPB6_07685"
                     /inference="COORDINATES: protein motif:HMM:NF037510.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR1184382.1"
                     /translation="MNETMPISAGLPAGMTTDACLEAQLRSAFLTGETDGEEAGLRPE
                     FVINDPDRRIKVLTVIEDELARCTSFDISVAFVTMSGIEPLLPIFEELRERGVPGRVL
                     TTDYLCFSDPAAMRKLAGIFNIEVRLFRAERNRGFHTKGYIFNSGDVCRFLIGSSNLT
                     GTALATNREWNARLVSKQTGAFARALQAEFRALWDHPLSRPLHEVIDAYEAERRAHMA
                     AQTEVMRRLPLPVSPVRPEPNSMQRAFVENLLRIAGSGERRALLISATGTGKTYAAAF
                     AVRELKPRRMLFVVHREQIARQAMASFRRVLGEDELTYGLLGGGQNEAGADCVFATMQ
                     TLARERVLGALDPKAFDVIIIDEVHRAAAESYKKIMAHFEPKLWFGMTASPDRPDGAD
                     IYALFDHNIAYEIRLSTALEEDLLCPFHYFGVKDVTVGGMMLEDLSDFRLLTAEERVS
                     NILDRAQYFGWSGPRVKGL"
     gene            1933..2940
                     /locus_tag="ACZPB6_07690"
     CDS             1933..2940
                     /locus_tag="ACZPB6_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HIR1184383.1"
                     /translation="MKVLAKMSQFVNKTFALWVVIFGIAGFVFPEIFKQIGPWIAPLL
                     GVVMFGMGLTLSPSDFREIFRRPRDVFLGILAQFTIMPGGAFLLCYALDLPPDVAVGL
                     MLLGCCPGGTASNVVTFLARGDVALSVTITSCTTLLAPVMTPALMYLFASQWLAIDPT
                     AMFMSIVQIILLPIAAGVFVHKLLGARIDTAVAALPLVSVLAIVLIAAAVTAASRQQL
                     MNTGLLVFVAVVLHNALGMLFGFLVAKWSGMNLAKCKCMAFEVGMQNSGLGVALASVH
                     FAANPMTALPAAIAALWHNVAGPMLATVFQNWRDDDEKASFFDRMEAACKTPAKAAAA
                     E"
     gene            complement(3105..>4292)
                     /locus_tag="ACZPB6_07695"
     CDS             complement(3105..>4292)
                     /locus_tag="ACZPB6_07695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184384.1"
                     /translation="TKDIFRVGKNRNDPVLVKQVTERVGGVVDWLVYDLKIPYGPAAT
                     QYPDHSANRQLGVQGRSVNYLNLMKGKFLDMGGKLMLETRAEELLRDDAGRVVGVRAK
                     DAQGNTVELTSKSVILASGGYGAVKSMLPKEMSNYVFYGLDSETGDGYRMATAIGADT
                     INMDLVKMYPQGVETVPGHGLAATASSTDTMKKSGAIYVNRDGRRYVNERASLGELTD
                     TTVAQPGHIAYIVMDAKAWKEYVAKSLEDKLVPDETALMKWTKIVNNGRPVMAVADTL
                     SEAASVMGVDAKGLEATVRDWNKMVKQGKDTAFGRQITGGIGEGPYYIVEQKVRYQTT
                     LGGLRAGEGLRILDKSGKPIPGLFGAGCVVGGANGADSMTAMMNSWAIVSGVIAAETA
                     VGK"
BASE COUNT          717 a         1465 c         1271 g          839 t
ORIGIN      
        1 aggcccttga cgcgcggacc gctccagccg aagtactgcg cgcggtcgag aatgttcgag
       61 acgcgctcct cggccgtcag cagcctgaag tcggagaggt cctcgagcat catgccgccc
      121 accgtgacgt ccttcacgcc gaaatagtgg aacggacaga gaaggtcctc ctcaagcgcc
      181 gtgctcaggc ggatctcgta ggcgatgttg tggtcgaaga gtgcgtagat gtccgcgccg
      241 tcgggcctgt cgggacttgc cgtcatgccg aaccagagct tcggctcgaa gtgcgccatg
      301 atcttcttgt agctctcggc cgctgcgcgg tgcacctcgt cgatgatgat cacgtcgaac
      361 gccttcggat ccaacgcgcc gagcacgcgt tctcgcgcaa gcgtctgcat ggtcgcgaag
      421 acgcaatccg cgcccgcctc gttctgcccg ccgccgagaa gcccgtaggt gagttcgtcc
      481 tcacccagga ctcgcctgaa gcttgccatc gcctgcctcg ctatctgctc gcggtgcacg
      541 acgaagagca tcctgcgggg cttgagctca cgcacggcga aggccgcggc ataggtcttg
      601 cccgtgcccg tggcggaaat gagcagcgcg cggcgctcgc ccgaacccgc gatcctgaga
      661 agattctcga cgaaggcgcg ctgcatcgag ttgggctcgg gacgcacggg cgaaacggga
      721 agcggcaggc gcctcatcac ctcggtctgg gccgccatgt gcgccctgcg ctcggcctcg
      781 taggcgtcga tcacctcatg gaggggacgg ctgagcggat ggtcccagag cgccctgaat
      841 tcggcctgaa gcgcccttgc aaaggcaccc gtctgcttcg agacgagcct tgcgttccac
      901 tcgcggtttg tcgcgagcgc cgtgcccgtc aggttcgagc ttccgatcag gaagcggcag
      961 acgtcgcccg aattgaagat gtagcccttc gtgtgaaagc ctcggttcct ctcggccctg
     1021 aagagcctca cctcgatgtt gaagatgccc gcgagcttgc gcatggcggc gggatccgaa
     1081 aagcagagat agtccgtggt cagcactcgt cccggcacgc ctcgttcgcg cagctcctca
     1141 aagatgggca gaagcggctc gatgccgctc atcgtcacga aggcaacact gatgtcgaag
     1201 ctcgtgcatc gcgcgagttc gtcctcgatc acggtgagga ccttgatcct gcggtcgggg
     1261 tcgttgatga cgaattccgg ccgaagtccg gcctcctccc cgtcggtctc tcccgtgagg
     1321 aaggccgagc ggagctgcgc ctcaagacag gcgtccgtcg tcatgccggc gggaaggccg
     1381 gcggaaatgg gcatggtctc gttcatgcca ggattctagc agccgctgcg cccgcccaaa
     1441 cgcctcgatg cgcctgcggc gcaggcccgg gcctccgaat cagggcagtc tgccggtccc
     1501 ggcccaggag agaggcgaag tcgggcgaat aggggttttc ccggaacacg cgtcaaatcg
     1561 acatccgcgt ggtcctgacc aagcctttgc ggaacaggaa cggtgcgggg ccctgacttt
     1621 ccacacgctt ccgatgattc cgaatcatct aggcaaacct ccctaagaga atcgtccgag
     1681 gcaccttgcg tgcagtcact actcccccgc tttccctgtg tcctaggcaa ttcgccctag
     1741 agacggcgga ttttccttcg gataaattgg aatcatcccg acgcccacaa ccttttttcc
     1801 acccgcggcg ccgggatcac cacggaagcc ggaccgtgca agcggtccgg aaggaaacca
     1861 tgaaatcctt ttttcacccc ttcgccacat ggagcggcct gaccgaataa gtcggagccg
     1921 acgcaaatca tgatgaaagt cctcgcaaaa atgagccagt tcgttaacaa gacgttcgcc
     1981 ctctgggtcg tcattttcgg cattgccggc ttcgtttttc cggagatttt caagcagatt
     2041 ggtccctgga tcgctcctct gctcggcgtg gtgatgttcg gcatgggcct cacgctctcg
     2101 ccctcggact tccgtgaaat cttccgccgt ccccgcgacg tcttcctcgg catcctcgcc
     2161 cagttcacga tcatgcccgg cggcgcgttc ctgctctgct acgcgctgga tctgccgccc
     2221 gacgtcgccg tcggcctgat gcttctgggc tgctgcccgg gcggcacggc gtccaacgtc
     2281 gtgaccttcc tcgcccgcgg cgacgtcgcc ctctcggtca ccatcacgtc ctgcacgacg
     2341 ctgctcgccc ccgtcatgac gcccgcgctg atgtatctct tcgccagcca gtggctagcg
     2401 atcgatccga ccgcgatgtt catgtcgatc gtccagatca tccttcttcc gatcgccgcc
     2461 ggcgtgttcg tccacaagct cctcggcgcc cgcatcgaca ctgccgtcgc agccctgccg
     2521 ctcgtatcgg tcctcgccat cgtgctgatc gccgcagccg tcacggcggc gtcccgtcag
     2581 cagctgatga acaccggcct tctggtcttc gtcgcagtcg tcctgcacaa cgccctcggc
     2641 atgctcttcg gcttcctcgt cgccaagtgg tccggcatga acctcgccaa atgcaagtgc
     2701 atggccttcg aggtcggcat gcagaactcg ggcctcggcg tcgccctcgc ctccgttcac
     2761 ttcgcagcca atccgatgac cgcactcccc gccgccatcg ccgccctttg gcacaacgtg
     2821 gccggcccga tgctcgccac cgtgttccag aactggcgtg acgacgacga aaaggcctcc
     2881 ttcttcgacc gcatggaagc ggcctgcaag acgcccgcca aggctgccgc cgccgaataa
     2941 caaggctcga atccaccact tcccaaaggc cgcgacgatt tctggtttcc cgtcgcggcc
     3001 tttgtgcatc cgccgaaaac accgcgggcc gctcatccga acaggatgaa cggccctcat
     3061 ggccttcatg atgccgcccg acgcacgggc ggctccttca cgacttactt gccgacggcc
     3121 gtttcagccg cgatgacgcc cgagacgatg gcccaggagt tcatcatggc ggtcatggag
     3181 tccgcgccgt tggcgccgcc cacgacgcag ccagcaccga agagacccgg aatcggcttg
     3241 ccgctcttgt caaggatgcg caggccttcg cccgcacgca ggccgccgag cgtcgtctga
     3301 taacggacct tctgctcgac gatgtagtac ggcccctcgc cgatgccgcc cgtgatctga
     3361 cggccgaagg cggtgtcctt gccctgcttc accatcttgt tccagtcgcg caccgtagcc
     3421 tcaagtccct tggcatcgac gcccatcacg ctcgcagctt cggagagcgt gtccgcaacg
     3481 gccatgacgg gacggccgtt gttgacgatc ttcgtccact tcatgagcgc ggtttcgtcg
     3541 ggcacgagct tgtcttcaag cgacttcgca acgtattcct tccaggcctt cgcatccatc
     3601 acgatgtagg cgatgtggcc gggctgagcc accgtcgtgt cggtgagttc gcccagagag
     3661 gcacgctcgt tcacatagcg cctgccgtcg cggttgacgt agatggcgcc cgacttcttc
     3721 atcgtgtcgg tcgagctcgc ggtggccgca agaccatggc ccggcaccgt ctcgacgccc
     3781 tgcgggtaca tcttgacgag gtccatgttg atcgtgtcgg cgccgatcgc cgtggccatc
     3841 ctgtagccgt cgcccgtttc gctgtcaagg ccgtagaaga cgtagttgct catctccttc
     3901 gggagcatgc tcttcactgc gccgtagccg cccgaggcga gaatcacgga cttggacgtc
     3961 agctcgacgg tgttgccctg cgcatccttg gcgcgcacgc ccacgacgcg gccagcgtcg
     4021 tcacgaagaa gctcttcggc gcgcgtctca agcatgagct tgccgcccat gtcgaggaac
     4081 tttcccttca tcaggtttag gtagttgacc gatcggccct gaacgccgag ctggcggttg
     4141 gccgaatggt cgggatactg cgttgccgcg ggaccgtacg ggatcttcag gtcgtagacg
     4201 agccagtcca cgacgccgcc cacgcgttcg gtcacctgct tgacaagaac cggatcattg
     4261 cggttcttgc cgacgcggaa gatgtccttc gt
//
LOCUS       DBMDTI010000167         4279 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4840_length_4279_cov_2.389899, whole genome shotgun sequence.
ACCESSION   DBMDTI010000167 DBMDTI010000000
VERSION     DBMDTI010000167.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4840_length_4279_cov_2.389899"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="ACZPB6_07700"
     CDS             complement(<1..296)
                     /locus_tag="ACZPB6_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184385.1"
                     /translation="MQVPQTESPSEFTTSAVLTAYKALPESLRPFYAHIHCGSAAIVA
                     ALGLTAEDNNVLETAVKAIGDLADDAEAFDRDAAAESGTLADMIRELAGTLGDD"
     gene            complement(417..887)
                     /locus_tag="ACZPB6_07705"
     CDS             complement(417..887)
                     /locus_tag="ACZPB6_07705"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050355 - inorganic triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIR1184386.1"
                     /translation="MGIEIERKFLVKSDGWRKLGCAVRYAQGYLCSDRNRTVRVRTVG
                     DKGFLTVKGRGSGIAHPEYEYEIPYNEARTMLDELAEKPVIAKTRTKIPFAGFVWEVD
                     EFFGDNEGLVMAEIELPSVDASFEKPEWIGEEVTGDPRYYNSMLARNPYKNWRR"
     gene            complement(915..>4279)
                     /gene="mfd"
                     /locus_tag="ACZPB6_07710"
     CDS             complement(915..>4279)
                     /gene="mfd"
                     /locus_tag="ACZPB6_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR1184387.1"
                     /translation="LSQMAEKCRTRHERLLVICADPLDAVRLGQEIPWFSPELGVRSL
                     PDWETLPYDVLSPQEDIVSERLETLYRMTSGTKGLDVVIASAVTAAQRMAPVAYVGAN
                     TFFFRPGDTLSVEGLCSNLAAAGYAGVKQVLAAGEFAVRGSIIDVFPMGSDRPFRLDL
                     FDDEIESIRWFDVETQRSMEAVNEIRLLPGHEFPVTPEAVTGFRQRWRTRFAGDPGKS
                     LVYRDIGNGVLPPGIEYYLPLFFEQTATLADYLSAGTRVVLVGDVQTALAGFMKETLS
                     RQRMLETDADRPAMKPEELWLTPEAFFTSLSKFARFTFRREGAPEAALALKGVAVDRK
                     SQNPVSNLIAFTTTEKTRGRRTLIVATSVGRMGTMGELFRASGLEFTEYENWADFLAG
                     DAPVGLAAAPLFAGSLLENPGTAILTETELYAASPRRSRLRRNRQSTNIEMIVRDVSE
                     LKIGDAVVHLEHGIGRYQGLVHMDTPDGEAELLRIDYKNEAKLFVPIANLHLISRYSG
                     ADPENAPLHALGRGDWEKARRKAAEQVRDTAAELLHLYALRETRKGVVFPIDISDYEA
                     FCEGFAFDETPDQAAAIGAVLNDMTTGKTMDRLVCGDVGFGKTEVALRAAFVAVSAGR
                     QVAVLCPTTLLAEQHAQTFRDRFAPWPVRVAELSRFRTGKETTKALEGIADGTVDIVI
                     GTHKLLTDKVSFKRLGLVIIDEEHRFGVRQKETLRKLRAEVDVLTLTATPIPRTLSMS
                     LEGIRDFSVIATAPERRLAVKTFVHAEEPGLIREAVIRELKRGGQVYFLHNEVETIEN
                     RREQLAALIPEARIAVAHGQMSERELEHIMREFYQQRYNLLLCTTIIETGIDVPTANT
                     IIMQRADKLGLAQLHQLRGRVGRSHHQAYAYLLTPPSGATTKQAKQRLEAIQSLEELG
                     SGFYLAMHDLEIRGAGEVLGEHQSGEIAEVGFDLYSRMLANAVKALKRGELPDFTEPL
                     VPTTEISLHAPALLPSSYVSDVSQRLGFYKELSSATSVEDIIRTTESLADRYGKLPEA
                     AQRLTQIHRLRLRCEALGVRKIDCADTATVITFTARPAVEPMALIRFLQSRKDARMLG
                     PDKLRIDSGGATPENRLALVQTVLAALEAPGR"
BASE COUNT          664 a         1292 c         1518 g          805 t
ORIGIN      
        1 ctgtcgtctc cgagcgttcc cgcgagctcg cgaatcatgt ctgcgagggt tccgctctcg
       61 gcggccgcgt cacggtcgaa ggcttccgca tcgtcggcaa ggtctccgat ggccttgacg
      121 gcagtctcca gaacgttgtt gtcttcagcc gtcaggccga gtgcggcgac gatggcggcg
      181 gatccgcagt ggatgtgcgc atagaagggg cgcagcgatt cgggaagcgc cttgtaggcg
      241 gtcagaacgg cggaagtcgt gaactcggag ggcgattcgg tttgaggtac ctgcatgggg
      301 aatccttcga ttgaaaaaac tgccagactg aaagtatgcg acaaatcgcc ggcattcgcg
      361 tgcttggcag ggtaatgcct gagattgcgc gggttgggca ctgcttgttt ttgggatcag
      421 cgccgccagt ttttgtacgg attccttgcg agcatcgagt tgtagtagcg cggatcgccc
      481 gtcacctcct cgccgatcca ctcgggcttc tcgaaggaag catccacgga tggcagctcg
      541 atctcggcca tgacgaggcc ctcgttgtcg ccgaagaatt cgtccacctc ccagacgaag
      601 cccgcaaacg gaatcttcgt gcgggttttg gcgatgacgg gcttttcggc gagttcgtcg
      661 agcatggtcc gggcctcgtt gtacgggatc tcgtactcgt actcgggatg cgcgatgccg
      721 cttccgcgtc ccttgaccgt gagaaagccc ttgtcgccca cggttcgcac gcgcaccgtg
      781 cggttcctgt cgctgcagag gtagccctga gcgtagcgca cggcgcagcc gagcttccgc
      841 cagccgtccg acttcacgag aaacttgcgt tcgatttcaa tgcccatgat tccggtccct
      901 ccattcgatt cagttcatcg acctggcgct tcgagcgccg cgagcacggt ctgcacgagt
      961 gcgaggcgat tttcgggcgt tgcgccgccc gagtcgatcc tcagcttgtc gggcccgagc
     1021 atgcgggcgt ccttcctcga ctgcaggaag cggatgagtg ccatcggctc gacggcgggc
     1081 cttgccgtga atgtgatgac ggtcgccgtg tcggcgcagt cgatcttgcg cacgccgagc
     1141 gcttcgcagc gaaggcgcag cctgtggatc tgagtgagtc tctgcgcggc ctcgggaagc
     1201 ttgccgtagc ggtcggcgag cgattcggtc gtgcggatga tgtcctcgac actcgtcgcg
     1261 gacgagagct ccttgtagaa gccgaggcgc tgcgagacgt ccgagacgta gctgctcggc
     1321 agcagtgcgg gcgcatggag cgagatttcc gtggtcggca cgaggggctc cgtgaagtcg
     1381 ggaagttctc cgcgcttgag tgccttgacg gcattggcga gcatgcggct gtagaggtcg
     1441 aacccgacct cggcgatctc gcccgactgg tgttcgccta gcacttcgcc cgcgcctcgg
     1501 atctcgaggt cgtgcatggc gaggtagaag ccgctgccga gttcctcaag ggactggatg
     1561 gcttcgaggc gctgcttcgc ctgcttcgtc gtggcgcccg agggcggggt gagcaggtag
     1621 gcgtaggcct gatggtggct tcggccgacg cggccgcgca gctggtggag ctgcgcgagg
     1681 ccgagcttgt cggcgcgctg catgatgatc gtgttggcgg tcgggacgtc gatgcccgtc
     1741 tcgatgatgg tcgtgcagag gagcaggttg tagcgctgct ggtagaactc gcgcatgatg
     1801 tgttcgagct cgcgctcgct catctgtccg tgcgcgacgg cgatgcgcgc ttcgggaatc
     1861 agtgcggcga gctgctcgcg gcggttctcg atcgtctcca cctcgttgtg caggaagtag
     1921 acctggccgc cgcgcttgag ttcgcggatg acggcctcgc ggatgaggcc cggctcctcg
     1981 gcgtgcacaa aggtcttgac cgccagacgg cgctcggggg ctgtcgcgat gacggagaag
     2041 tcgcggatgc cttcgaggct catcgagagc gtgcgcggga tcggcgtggc cgtgagcgtg
     2101 aggacgtcga cctcggcgcg cagcttcctg agcgtctcct tctggcgaac gccgaagcgg
     2161 tgttcctcgt cgatgatgac gaggccgagg cgcttgaagg acaccttgtc ggtaagaagc
     2221 ttgtgcgtgc cgatcacgat gtcgaccgtg ccgtcggcga tgccttcgag cgccttcgtc
     2281 gtctctttgc ccgttctgaa gcgggagagc tcggcgacgc gcacgggcca gggcgcgaat
     2341 cggtcgcgga aggtctgggc atgctgctcg gcgagcaggg tcgtcgggca gagcacggcc
     2401 acctgccggc ccgccgagac ggcgacgaag gccgcgcgca acgcgacttc ggtcttgccg
     2461 aagcccacgt cgccgcagac aaggcggtcc atcgtcttgc ccgtcgtcat gtcgttgagg
     2521 acggcgccga tggcggccgc ctggtcgggc gtctcgtcga atgcaaagcc ctcgcagaag
     2581 gcctcgtagt cgctgatgtc gatggggaag acgacgccct tgcgggtctc gcgtagcgca
     2641 tagaggtgca ggagctcggc cgccgtgtcg cggacctgct cggccgcctt ccttctcgcc
     2701 ttttcccagt cgccgcgtcc gagagcgtgc agcggggcgt tctcgggatc ggcgcccgag
     2761 tagcgcgaga tgaggtggag gttggcgatc ggcacgaaga gcttggcctc gttcttgtag
     2821 tcgatgcgca gaagctcggc ctctccgtcg ggcgtgtcca tgtgcacgag cccctgatag
     2881 cggccgatgc cgtgctcgag gtgcacgacg gcatccccaa tcttgagttc ggagacgtcg
     2941 cgcacgatca tttcgatgtt ggtcgactgg cggttgcggc gcagacgcga acgcctcggg
     3001 ctggcggcat agagctcggt ttcggtgagg atcgccgtac cgggattctc aaggaggctg
     3061 cccgcaaaga gcggcgcggc cgcaaggccc acgggcgcgt cgcccgcgag gaagtccgcc
     3121 cagttttcgt actccgtgaa ttcgaggccc gacgccctga agagctcgcc catcgtgccc
     3181 atgcggccca ccgaggtcgc aacgatgagc gtgcggcggc cccgcgtctt ctcggtcgtc
     3241 gtgaaggcga tgaggttcga gacggggttc tggctcttgc ggtcgaccgc aacgcccttg
     3301 agggcgagcg cggcttcggg ggctccctcg cgcctgaagg tgaagcgcgc gaacttcgag
     3361 aggctcgtga agaatgcttc gggcgtgagc cagagctcct cgggcttcat cgcgggacgg
     3421 tccgcgtccg tttcgagcat gcgctggcgc gagagcgtct ccttcatgaa gccagcaagc
     3481 gcggtctgca cgtcgcccac gagcacgacg cgcgtgcccg ccgacagata gtcggccagc
     3541 gtggccgtct gctcgaaaaa gagcggcaga tagtattcga tgcccggcgg caggaccccg
     3601 ttgccgatgt cgcggtagac gaggcttttg cccggatcgc ctgcaaagcg cgtgcgccag
     3661 cgctgcctga agcccgtgac ggcctcgggc gtcacgggga actcgtggcc cggaagcagc
     3721 ctgatctcgt tgacggcctc catggagcgc tgcgtctcga cgtcgaacca gcggatcgac
     3781 tcgatctcgt cgtcgaagag gtcgaggcgg aagggcctgt cggagcccat cgggaagacg
     3841 tcgatgatgc tgccgcgaac ggcgaattca cccgccgcga gcacctgctt gacgcctgcg
     3901 tagcctgcgg cggcgaggtt cgagcaaagg ccctcgaccg agagcgtgtc gccgggacgg
     3961 aagaagaagg tgttcgcgcc cacgtaggcg acgggcgcca tgcgctgggc ggccgtgacg
     4021 gcggaggcga tcacgacgtc gaggcccttc gtgcccgagg tcatgcggta gagggtctca
     4081 aggcgctcgg agacgatgtc ctcctgcggg gagagcacgt cgtaggggag cgtctcccag
     4141 tcggggaggc ttctgacgcc gagttcggga ctgaaccaag ggatctcctg cccgagcctc
     4201 acggcgtcga gcggatcggc gcagatgacg aggagtcttt catgccttgt gcggcacttc
     4261 tcggccatct gcgagagcg
//
LOCUS       DBMDTI010000168         4266 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4856_length_4266_cov_2.271309, whole genome shotgun sequence.
ACCESSION   DBMDTI010000168 DBMDTI010000000
VERSION     DBMDTI010000168.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4856_length_4266_cov_2.271309"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="ACZPB6_07715"
     CDS             <1..485
                     /locus_tag="ACZPB6_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1184388.1"
                     /translation="WVIVDSTCPVLDKVIPKFDRLNSPYGKADTIEDLCKQVKLPVEK
                     VVKTIKEYNEAVKSGKLGEMNPPCTYKKPHEVAKAPFYAVPFQGGMTATFGGPLINVK
                     AEIQNLDGTSIPGLYAVGNSAGGIFFHNYAGGAQLGAATVFGRIAGNEIAARAKAQKN
                     "
     gene            503..826
                     /locus_tag="ACZPB6_07720"
     CDS             503..826
                     /locus_tag="ACZPB6_07720"
                     /inference="COORDINATES: protein motif:HMM:NF025890.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR1184389.1"
                     /translation="MTKSITMALVATLGLGLAFGACAAEKTLAEVHGGQLPAETGWAT
                     KGQCLQCHQSYDALAEKTKNFEPNPHKSHLGAVNCVECHKADKPSKQPELMCNSCHNF
                     TLKTK"
     gene            1023..1745
                     /gene="nfsA"
                     /locus_tag="ACZPB6_07725"
     CDS             1023..1745
                     /gene="nfsA"
                     /locus_tag="ACZPB6_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HIR1184390.1"
                     /translation="MNDTIRTQLAHSSVRAFTNEPVTAELRTQILDAARAASTSCALQ
                     VTSIIRVTDPALRARIAELSGGQAHVAAAPEFWVFCADYHRLQLLCPEADLGWIEQFL
                     VGCLDTGIMAQNAFTALESLGLGGCFIGGIRNGITEVSALLRLPQNVFPVVGIAFGHP
                     AVRNPVKPRLPATITFMENGYEEPAPAELDAYDATMRAYYAGRGSNAREATFRDSIHP
                     VIARERRRFLLDYLQTQGFALK"
     gene            1824..2315
                     /locus_tag="ACZPB6_07730"
     CDS             1824..2315
                     /locus_tag="ACZPB6_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HIR1184391.1"
                     /translation="MKTKLLAAFAAASVLALAGCSGGDEVGDVSLGLFTLKDIKINTF
                     QDPKVPGVTCHVASIEANLSLADPSDSSVSCRQTGEITPEMIASIDRSKSGEVVFTKS
                     KSILLKTMKIRRIYDAETQTLLYISYSTKETTGSFKHSLSTIPLWGTAAWENRPPVPA
                     KTM"
     gene            complement(2479..4221)
                     /gene="purL"
                     /locus_tag="ACZPB6_07735"
                     /gene_synonym="purI"
                     /pseudo
     CDS             complement(2479..4221)
                     /gene="purL"
                     /locus_tag="ACZPB6_07735"
                     /gene_synonym="purI"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
BASE COUNT          808 a         1428 c         1323 g          707 t
ORIGIN      
        1 cctgggtcat cgtcgacagc acctgcccgg tcctcgacaa ggtcattccg aagttcgacc
       61 gcctcaacag cccgtacggc aaggccgaca cgatcgaaga tctctgcaag caggtcaagc
      121 tcccggtcga aaaggtcgtg aagacgatca aggaatacaa cgaagccgtc aagtccggca
      181 agctcggcga aatgaacccg ccctgcacct acaagaagcc ccacgaagtc gccaaggctc
      241 cgttctacgc cgtgcccttc cagggcggca tgacggccac cttcggcggc ccgctcatca
      301 acgtcaaggc tgaaatccag aacctcgacg gcacgagcat cccgggcctc tacgcggtgg
      361 gcaactccgc tggcggcatc ttcttccaca actacgcagg cggcgcacag ctcggtgccg
      421 cgacggtctt cggccgcatc gccggcaacg aaatcgccgc ccgcgcaaag gcccagaaga
      481 actaatttcc ggagtatcaa tcatgaccaa atccatcacc atggcgcttg tcgccaccct
      541 cggtctgggc cttgccttcg gcgcctgcgc tgctgaaaag accctcgccg aagtccacgg
      601 cggccagctt cccgccgaaa ccggctgggc cacgaagggc cagtgcctcc agtgccacca
      661 gagctacgac gccctcgccg aaaagacgaa gaacttcgag cccaacccgc acaagtcgca
      721 cctcggcgcc gtcaactgcg tcgaatgcca caaggccgac aagccttcca agcagcccga
      781 gctgatgtgc aacagctgcc acaacttcac gctcaagacg aaatgatctc ctcttgaacc
      841 atcagggttc ttccttcgga cgcgccgtgt ggggtgcgtc cgggaaacca gaccgaccgc
      901 aaggcggtcc ttcgcccgcg tcccttgagg ccgcgggctt ttttcgttca gattcgtgaa
      961 cggcggtact cccgcacggg caagaaggct agactcgatg catcaccctt aaggagttca
     1021 taatgaacga cacgatccga acccagctcg cgcacagctc cgtgcgcgcc ttcaccaacg
     1081 agcccgtcac ggccgaactg cgcacgcaga tcctcgatgc ggcgcgcgcc gcgtccacat
     1141 cctgcgcact gcaagtgaca agcatcatcc gcgtcaccga tccagcactt cgagctagga
     1201 tcgcggagct ctcgggcggt caggcccatg tcgcagccgc acctgaattc tgggtcttct
     1261 gcgccgacta ccaccgcctt cagctgctct gccccgaggc cgacctcggc tggatcgagc
     1321 agttcctcgt cggctgcctt gacacgggca tcatggccca gaacgccttc acggcgcttg
     1381 aatccctcgg gctcggcggc tgcttcatcg gagggatcag gaacggcatc acagaggttt
     1441 ctgcgcttct gcgcctgccg cagaacgtct ttcccgtcgt cggcatcgcc ttcgggcatc
     1501 ccgccgtgcg caatcccgtc aagccgcggc tgcccgccac catcaccttc atggaaaacg
     1561 gctacgaaga acccgcgcca gccgaacttg acgcttatga cgcaacgatg cgagcctact
     1621 acgctggacg cggaagcaat gcccgcgagg cgaccttccg cgactctatc catcccgtca
     1681 tcgcccgcga gcgccgcagg ttcctgctcg attatctgca gacgcagggc ttcgcgctga
     1741 agtagtacgc ccaggacagg acgacgcccg cccgccatat ataatgacga caccgtaaac
     1801 caaatccgac aaggaccaca cgcatgaaga ccaagcttct cgcggccttc gctgccgcct
     1861 ccgttctcgc tctcgccggc tgctcgggcg gcgacgaagt gggcgacgtc tcactcggct
     1921 tgttcacgct caaggacatc aagatcaaca ccttccagga tccgaaggtt ccgggcgtca
     1981 cctgccatgt cgcaagcatt gaggccaacc tctcgcttgc ggacccgtcc gacagctcag
     2041 tctcctgccg ccagacgggc gaaatcacgc ccgagatgat cgcctcgatc gaccggtcga
     2101 agtcgggaga agtggtcttc acgaaatcga agagcattct gctcaagacg atgaagatcc
     2161 gccgcatcta cgacgccgag acgcagacgc tcctttacat ctcctactcc accaaggaga
     2221 cgacgggcag cttcaagcac agcctctcga cgatcccgct ctggggaacc gccgcctggg
     2281 aaaaccgccc gccggttccc gcgaagacga tgtgattttc tggaaacgcg aacgccctgc
     2341 aggacacgaa gacctgcagg gcgttttcac atttgagcgt caccctgtga aaagggcccc
     2401 gaatcggggc cccatctctt tccgtcgtcc tccatcagga gggcccgaag gccctccgtc
     2461 gggagcggag ccggcttctt agccgaccca cttgcgggcg ttgcggaaca tgcgcatcca
     2521 gcccgaggcg tccgtccatt cggccgggtg ccaggagagc tgctgggcgc ggtgcgagcg
     2581 ctccggatgc ggcatcatga tcgtgaagcg gccgtcttcg gtcgtcacgg acgtgaggcc
     2641 gcccttggag ccgttcgggt tgtacggata gacctcggtc gggttgccgc acggatcaac
     2701 gaagcgggcg gcggccttca cgagcgcggc gtcttccgga cgcaggaact gcacgcggcc
     2761 ttcgccgtgg gagttcacga cgggcatcac gctgccggcc atgcccgcga agaagatcga
     2821 cgggctctcg agaacctcga cgttcacgag acgggcctcg aactgctcgg acgtgttgcg
     2881 cacgaattcc ggccagtgag aggcgcccgg gatcaggtcg cgcagatggc tcatcatctg
     2941 gcagccgttg cagatgccca gaccgaaggt gtcctcgcgg ttgaagaacg tgcggaacat
     3001 ttcgaccatg cggtcgttgt gaaggatggt cttggcccag ccgccgccag cgccgagcac
     3061 gtcgccgtag gagaagccgc cgcagcaggc aaggcccgtg aattcggcga ggtcggcgcg
     3121 gcccgtgagg aggtccgtca tgtgaacgtc ataggcgtcg aagccggcgc gcgtgaacgc
     3181 agcggccatt tccgtctggg agttgacgcc ctgttcgcgc aggatggcga tcttcggacg
     3241 cacgcccgtg aggatcatcg gagcggcgac gtcttcattg acgtcgaagg tcgtctttgc
     3301 gaagaggccg gtgtcctcct tgcgctcgat gcgggcgaac tcgctgtcgg cgcaggcggg
     3361 attgtcgcgg ccgcgggcga tctgccagga gacttccgtc caggccttct ggaggtcagc
     3421 acggtcgagt cgggcgagtt cggcaccgtt ggcgctgatc gtgagcgcat cgtcgtcgac
     3481 gacttcaccc acgaagtggc agacggaggc aagaccggct tcgcgcatca cttcggccac
     3541 gcgggcggcc ttctcggccg gcacctgcat caggccgccg agctcttcgt tgaagagagc
     3601 ggcgaagacg tcggcgtcct tcgtgaggga cgtcaggtcg agcttcacac cgaggcggga
     3661 ggcgaacatc atttcagagg ccgtggcggc gagaccgccg tcggcacggt cgtggtaggc
     3721 cacgacggcg ccttcattga cgagcgtgcg cagcgcgccc atgaagcggg cgagcatggc
     3781 gggatcctcg cagtcgggcg ccgtgtcgcc gaagcgctgg gcaacctggg cgaggaggga
     3841 gccgcccatg cgggcgcggc cgcagccgag gtccgcaagc acgagcaccg acgggatctt
     3901 cctgagttcg ggcgtaagcg tgagcgtcac gtcgccgacg ggagctgcgg cggaggcgat
     3961 gagcgagacg gggctcgtca cggacttctt ctcgccattg tcctcccagg cggtgcgcat
     4021 ggagagcgag tccttgccca cgggcacgga gatgccgagc gcaacgcaga agtcgctcgc
     4081 gcccttgacg gcgtcaaaga gcttggcgtc ctcgcccttg gcgccgcagg cggccatcca
     4141 gttgagggag agcttgactt cggggagctt cacgtcggcg gcggcgatgt tggtgagcgc
     4201 ttcgcccacg gccatgcggc tcgcagcagc ggcagttcat tatcaacggg attccctcga
     4261 gggatc
//
LOCUS       DBMDTI010000169         4264 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4859_length_4264_cov_3.073264, whole genome shotgun sequence.
ACCESSION   DBMDTI010000169 DBMDTI010000000
VERSION     DBMDTI010000169.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4859_length_4264_cov_3.073264"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            208..963
                     /locus_tag="ACZPB6_07740"
     CDS             208..963
                     /locus_tag="ACZPB6_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012610.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1184392.1"
                     /translation="MTRAAVRERAQARRAADAAFREAFDAYMFECFAKPGFKLESEAQ
                     LAERFGVTRYKVRKAIEALNQAGVLERVKHGGSTVRSVTPEELADRADRLLSVAGLPA
                     EEFEDAVSDLVCGLVPVIMDKVDPEKLAGLETLVETVGAARTPAERRSAVFDFLTGLV
                     AVDGNRYTALCVSLAVRQAARREAYDLRLDPAELAAVCRGVLKELMKGRRKKVVAELE
                     DLFGLVFPKRAELKKAEKTEKSEKAEKAEKGGK"
     gene            complement(1098..1703)
                     /locus_tag="ACZPB6_07745"
     CDS             complement(1098..1703)
                     /locus_tag="ACZPB6_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184393.1"
                     /translation="MSSKNQEKNDTPVNRPVDKIFAENLGYTFGGCVRDLSGSLFNKE
                     VAKAAGVSLCPIPLLGGEEKRRFKAFWAANLQAVAMRTAVENLPSYADEKLLKKTLFQ
                     MQTFVDQALGRPLFSKLSPEDLDRYSTIRSRMTQAALTPGADKESMARTFLALVHGTA
                     PDSVPDSRVSDTAGHIGMSMGLFKRLLDISLNSPNSWVRAK"
     gene            1975..2400
                     /gene="ndk"
                     /locus_tag="ACZPB6_07750"
     CDS             1975..2400
                     /gene="ndk"
                     /locus_tag="ACZPB6_07750"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR1184394.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGKIYSRFETNGLKIVAAQMRQLSQ
                     AEAEGFYAVHKERPFFKDLVSFMTSGPVMIQVLEGEGAILKNRELMGATDPKKAAAGT
                     IRADFAESIDANAVHGSDAPETAAVEIAYFFPALSIHSR"
     gene            2430..3602
                     /gene="rlmN"
                     /locus_tag="ACZPB6_07755"
     CDS             2430..3602
                     /gene="rlmN"
                     /locus_tag="ACZPB6_07755"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR1184395.1"
                     /translation="MTDNVKESAAASKVNLLDFDLEGLTAWCAGIGEKAFRATQLSRW
                     LHRHVEGDFDNMTNLAKVFRAKLKDMAEVRPPVPITEKKSADGTRKWLFDVGNGNAVE
                     SVFIPEDDRGTLCISTQAGCAMGCLFCSTGKQGFNRNLTTAEIVGQLWWAERELRRDA
                     GVTDPNDRIISNVVLMGMGEPLQNLDNVLCALHVFLDDNGYGLSRRRVTVSTSGLVRQ
                     MDKLAEDSPVALAVSLHAGNDALRDKLMPVNRKHPLADLMAACRRYLKVAPRDFITFE
                     YVMLGGINDRLEQADELVELVRRENVPCKFNLIPFNPFTQSDLRKPEREQVLAFCRRL
                     NEAGIVTTVRKTRGDDIDAACGQLAGEVRDRTRRAERLAQQKAEAAPLLQAARRSE"
     gene            3686..>4264
                     /locus_tag="ACZPB6_07760"
     CDS             3686..>4264
                     /locus_tag="ACZPB6_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024805.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1184396.1"
                     /translation="MAQETSDMNEPVLHPAPAEGFGAALKRAREAQGISIGDMAARSR
                     LSVQQVRALESERTAELPEPVYVRAFIRGVASVLGLEPDPLVADYTARYGAASVGVLP
                     DHDPAKETVVRASGRRTGLKAAVVAIAAVLVCAAGWYAWSSMSDGAETQPAAETPVVD
                     APESVEAAPAPEQAAADQTAQEKTDRLPPVQGR"
BASE COUNT          812 a         1382 c         1350 g          720 t
ORIGIN      
        1 tccaagagtc gaaggagtcg aaaatgtcga aaaaagcgcc ggacgccatc gcgtcgcggc
       61 gtttttttcg cattccggct catcgaacac gatgttttcc taaggccttt cacggcagga
      121 tcaaaaaacg ctcatgttaa gtcgaaatat gctcaacggg agggttacaa tgggcgaaca
      181 aagcccgtca tgcaaggagg aacaatcatg accagagccg cagtcagaga aagggcgcag
      241 gcccgccgcg cagccgatgc ggccttcagg gaggcgttcg acgcctacat gtttgaatgc
      301 ttcgccaagc ccggattcaa gcttgagagc gaggcgcagc ttgccgagcg tttcggcgtc
      361 acccgctaca aggttcgcaa ggcgatcgag gcccttaatc aggcgggcgt gctcgagcgc
      421 gtcaagcacg gcggctccac cgtgaggagc gtcacgcccg aggagcttgc cgaccgcgcc
      481 gatcgtcttc tgagcgtcgc ggggctgccc gccgaggagt tcgaggacgc cgtctccgat
      541 cttgtctgcg gcctcgtgcc cgtcatcatg gacaaggtcg accccgagaa gctcgcgggt
      601 cttgagaccc tcgtcgagac cgtgggcgcc gcacgcacgc ctgccgagcg ccgcagcgcc
      661 gtcttcgact tcctgacggg actcgtagct gtcgacggca accgctacac cgcgctctgc
      721 gtgagccttg ccgtcaggca ggccgcccgc cgcgaggcct atgacctgag gctcgatccc
      781 gccgagctcg ccgccgtctg tcgcggtgtt ctcaaagagc tgatgaaggg gcgccgcaag
      841 aaggtcgtgg ccgagctcga ggatctcttc ggactcgtgt tcccgaagcg cgccgagctg
      901 aagaaggccg aaaagacgga aaagtccgag aaggccgaga aggctgaaaa gggcggcaag
      961 taggactcgt cccggaaaag aaagaggggc gcaggcggca tggcgacgcc tgcgccccgt
     1021 ttttggcggg cgcaaaaaag gcggctcgat cgagccgccc gtcaggcgcc tgggcattca
     1081 ccttgcgcgg gatggcgtca cttggcgcga acccaggagt tcggagagtt gagcgagatg
     1141 tcgagcaggc gcttgaagag gcccatggac atgccgatgt ggccggcggt gtcgctcacg
     1201 cgggaatcgg ggacggagtc gggcgcggtg ccgtgaacga gcgcgaggaa ggtgcgggcc
     1261 atggactcct tgtcggcgcc gggcgtgagg gccgcctgcg tcatgcgcga gcggatcgtg
     1321 ctgtagcggt cgagatcctc gggggagagc ttcgagaaga gcggacgtcc gagggcttgg
     1381 tcgacgaagg tctgcatctg gaagagcgtc ttcttcagaa gcttctcgtc ggcataggag
     1441 gggaggttct cgacggcggt cctcatggcg acggcctgaa ggttggccgc ccagaaggcc
     1501 ttgaagcggc gcttctcctc gccgcctaga agcggaatcg ggcacaggct cacgccggcg
     1561 gccttggcca cctccttgtt gaagagcgag ccggagaggt ctcgcacgca gccgccgaag
     1621 gtgtagccga ggttttccgc aaagatcttg tcgacgggac gattgacggg cgtgtcgttc
     1681 ttttcttggt ttttggagga catgagctgg aatccgaaat gggtgtgccg gaaacctccg
     1741 tgcaggggag atttctcaac aggtgcaatt ctgccatgac tcgcacgcgg tgaaaaaggg
     1801 aaactaaccc tgatcgggtt gtagccaaaa gaaaaaggcg tgtgccccgg ggagtgattg
     1861 cgcggcctcg tgctaaaatc atcagttcag tacgaaaggg agcacaaccg ctcccttttg
     1921 tcggttgttc cacgtcccgg agccttttcc gggcattttc tctgagagaa caaaatggct
     1981 attgaacgca ccctctccat catcaagccg gacgccgttg ccaagaacgt catcggcaag
     2041 atctactccc gtttcgaaac gaacggcctc aagatcgtcg ccgcccagat gcgtcagctt
     2101 tcccaggctg aagccgaagg cttctacgct gttcacaagg aacgtccctt cttcaaggat
     2161 ctcgtctcct tcatgacgtc cggcccggtc atgatccagg ttctcgaagg cgaaggcgcc
     2221 attctcaaga accgtgagct catgggtgcc acggacccga agaaggccgc cgccggcacg
     2281 atccgcgccg acttcgctga aagcatcgac gccaacgccg ttcacggctc cgacgctccg
     2341 gaaacggccg ccgtcgaaat cgcctacttc ttcccggctc tctccattca cagccgctaa
     2401 tccgctgcga acacagtgag gaataattcg tgaccgacaa cgtcaaagag tctgccgccg
     2461 cctctaaggt gaaccttctc gacttcgatc tggaagggct caccgcttgg tgcgccggca
     2521 tcggcgaaaa ggccttccgt gcaactcagc tctcgcgctg gctccatcgt catgtcgaag
     2581 gcgacttcga caacatgacg aacctcgcga aggtgttccg agccaagctg aaggacatgg
     2641 ccgaagtgcg tcctcccgtg cccatcaccg agaagaagtc ggctgacggc acgcgcaagt
     2701 ggctctttga cgtcggcaac ggaaacgcgg tcgaatcggt gttcattcca gaggatgacc
     2761 gcggcacgct ctgcatctcc acgcaggcgg ggtgcgccat gggctgcctc ttctgctcca
     2821 cgggcaagca gggcttcaac cgcaacctca cgaccgccga gatcgtgggc cagctctggt
     2881 gggccgaacg cgaactgcgc cgcgacgcag gcgtgacgga ccccaacgac cgaatcatct
     2941 ccaacgtcgt gctcatgggg atgggcgagc cgttgcagaa cctcgacaac gttctgtgcg
     3001 ccctgcacgt cttccttgac gacaacggct acgggctctc ccgccgccgc gtcaccgtct
     3061 ccacgtcggg cctcgtccgc cagatggaca agctcgccga ggacagccct gttgcgctcg
     3121 ccgtgagcct gcatgcgggc aacgacgcgc tgcgcgacaa gctcatgccc gtcaatcgca
     3181 agcatccgct tgccgacctg atggccgcgt gccgccgcta cctcaaggtg gccccgcgcg
     3241 acttcatcac cttcgagtac gtcatgctcg gcggcatcaa cgatcgcctt gagcaggccg
     3301 acgaactcgt cgaactcgtg cgccgtgaaa acgtgccgtg caagttcaac ctgattccct
     3361 tcaatccctt cacgcagtcg gacctgcgca agcccgagcg cgagcaggtt ctcgcgttct
     3421 gccgccgcct caacgaggcg ggcatcgtga cgacggtccg caagacgcga ggggacgaca
     3481 tcgacgctgc gtgcggccag ctcgcgggcg aggtgcgcga ccgcacccgc cgcgccgagc
     3541 gccttgcaca gcagaaggcc gaggccgcgc ctcttctgca ggcggcccgc cgcagcgagt
     3601 agcctcccgc cggggcgtgc gcgagtcctg agggtccgcg gcgcccaaga caccgaacat
     3661 caaaacactc ctggaaggaa gcgccatggc ccaagaaacg tctgacatga acgagccggt
     3721 cctgcatccg gcccccgccg aaggcttcgg cgccgcgctc aagcgcgctc gcgaggccca
     3781 gggcatctcc atcggcgaca tggccgcccg ctcccgcctt tcggtccagc aggtccgcgc
     3841 gcttgagagc gaaagaactg ccgagcttcc cgagcccgtc tacgtgcgcg ccttcatccg
     3901 cggcgtcgcc tcggtgctcg gtctcgaacc cgatccgctc gtcgccgact acaccgcccg
     3961 ctacggcgcc gcaagtgtcg gggtccttcc cgaccatgat cccgccaagg agaccgtcgt
     4021 gcgcgcctcc ggccgccgca cgggcctcaa ggccgccgtc gtcgctatcg ctgcggtcct
     4081 cgtctgcgct gcaggctggt acgcctggtc gagcatgagc gacggcgccg agacgcagcc
     4141 cgctgcggag acgcccgtcg tcgatgcccc cgaatcagtt gaagcggcgc ccgcccctga
     4201 acaggcggca gccgatcaga ctgctcagga gaagactgat cggctgccgc ctgttcaggg
     4261 gcgg
//
LOCUS       DBMDTI010000170         4215 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4924_length_4215_cov_3.028439, whole genome shotgun sequence.
ACCESSION   DBMDTI010000170 DBMDTI010000000
VERSION     DBMDTI010000170.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4924_length_4215_cov_3.028439"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="ACZPB6_07765"
     CDS             <1..604
                     /locus_tag="ACZPB6_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1184397.1"
                     /translation="VPPELRKRHLALMRDQTARMKRLTDDLLMLSKLENRDERGTDQP
                     ELIDMSALIDDAVREGMAYSKMQHKFEVAHEPLSLLGHPEEIRSAVLNLITNAVRYTP
                     RGGRIRVVWKRTGTGAVLTVADTGIGIPPEHLPRITERFYRVDKGRSRDTGGTGLGLA
                     IVKHVMRRAGGELSIESTPGEGSKFMLRFPEERIFSGRYL"
     gene            708..1463
                     /gene="surE"
                     /locus_tag="ACZPB6_07770"
     CDS             708..1463
                     /gene="surE"
                     /locus_tag="ACZPB6_07770"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR1184398.1"
                     /translation="MHILVSNDDGYRAPGIQALAQAMRRFGRVTVVAPDHNHSGASNS
                     LTLNRPLTVEHQDGDSLYVLSGTPSDCVHVALTGLLPKPDLVVSGINCGANMGDDTMY
                     SGTVAAAIEGYLFGVPSIAFSQIDKGWAELDSAAKIAQLVVERYLESREDERAVLLNV
                     NMPNMPVEALKGIRGTRLGKRNCAESVIREMSPRGFPIYWLGAAGKPADAEEGTDFWA
                     TSHGYVSVTPLQVDLTNYKQVPTVRGWMDGPAV"
     gene            1518..2462
                     /locus_tag="ACZPB6_07775"
     CDS             1518..2462
                     /locus_tag="ACZPB6_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR1184399.1"
                     /translation="MNKTVRILSISVAAAAAAFLSGCSSVDMGPATVVDHSTYSDGAA
                     TAPVQTPSLLPGGIVDSGRTHTVAVGDTIYNISVRYGLSAEQLQQLNAITDPTQLRLG
                     QVLRLPASVNAPRAYTGNPQVRVNRVTNDTVLETAPIRNAETVPAAGETTATSSAASA
                     DAAEAARKKAEAEAAAEKVRTQAEAAKSQAQQKLNAVTPGQRMIWPVRGKIKSDFAKN
                     TFGLEIAGTQGDVVVAAMQGEVIWVGTLKGYSGTLAVVKHSPTLITAYGGLENVAVSQ
                     GSKIKSGQKIADLKGDTLRFEVRETGKCVDPLKYLNKR"
     gene            2608..4062
                     /gene="rlmD"
                     /locus_tag="ACZPB6_07780"
     CDS             2608..4062
                     /gene="rlmD"
                     /locus_tag="ACZPB6_07780"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1184400.1"
                     /translation="MARRKEKTPEYFTVEVERLDQEGRGVGHHDGKVVFIEGALPGEL
                     VTYERIRNKTSFEIGRVAQVHRESVQRVEPRCPKFGHGPGSCGGCAMQHLEPRAQVAY
                     KERTLIDTLWHIGRVRPEQIITPIYGPFWNYRHRARLTVRDVAKKGGVLVGFHEKSSS
                     YVAEMTGCHILTKNVSDMIMPMRELVSKLSIRQRMPQIEVAVGGNGRTALVFRTLEEP
                     TDGDMALLNAFGAEHKADIWLQPKGPDSAHPVDPVNINALGLQLPEFGVRINFKPTDF
                     TQVNHELNETMVSRAVRLLGVEPEHKVADFFCGLGNFTLPLATCASHVTGIEGSDALV
                     ARARQGAADNGLAEKTDFVARNLFTWCDQDWEALWKKMGGIDRVLIDPPREGALALSR
                     VLAATDKRPARIVYVSCNPATLARDCAILHHEGGWHIRAAGIMNMFPHTGHVESIAVL
                     EPGPRPEKKSEEAEAIERRIAASGDDDVKMNLPQ"
BASE COUNT          791 a         1502 c         1267 g          655 t
ORIGIN      
        1 cgtgccgccc gagctcagaa agcgccatct cgccctcatg cgcgaccaga cggcccgcat
       61 gaagcgcctc acggacgacc tgctgatgct ctcgaagctc gagaacaggg acgagcgggg
      121 gacagaccag cccgagctca tcgacatgtc cgcgctcatc gacgacgccg tgcgcgaggg
      181 catggcctac tcgaagatgc agcacaagtt cgaggtcgca cacgagcccc tttcgcttct
      241 cggacatccc gaggagatcc gaagcgcggt cctcaacctc atcaccaacg ccgttcgcta
      301 cacgcccaga ggcggccgaa tcagggtggt ctggaagcgc acgggcacgg gcgccgtcct
      361 cacggttgcc gacacgggca tcggcattcc gcccgagcac ctgccccgca tcaccgaacg
      421 cttctaccgc gtcgacaagg ggcgctcgag ggacacgggc ggcacgggcc tcggtctggc
      481 cattgtcaag cacgtcatgc ggcgtgcggg aggcgagctc tccatcgagt cgactccggg
      541 cgaaggctcg aagttcatgc tcaggttccc cgaggagagg atcttctcgg gccgctatct
      601 gtagaacgga cgcggcatcc tgcgatcgga gcccgttcgg gcgcgccctt gctggcgcga
      661 tgggctaaaa tcagacgatc caattcaccg aagtccgcag gatttccatg cacattcttg
      721 tctccaacga tgacggctac cgcgcgcccg gcattcaggc gctcgcgcag gccatgcgcc
      781 gtttcggccg cgtgacggtc gtcgcgcccg accacaacca ctccggcgcc tccaattcgc
      841 tcacgctcaa ccgtccgctc accgtcgagc atcaggacgg cgacagccta tacgtgcttt
      901 cgggcacgcc gtccgactgc gtgcacgtcg cgctcacggg tcttctgccg aagcccgacc
      961 tcgtcgtctc aggcatcaac tgcggcgcca acatgggcga cgacacgatg tactcgggca
     1021 cggtcgcggc ggccatcgag ggctacctct tcggcgttcc ctccatcgcc ttctcgcaga
     1081 tcgacaaggg ctgggccgaa ctcgactccg ccgcgaagat tgcgcagctc gtcgtcgagc
     1141 gttatcttga aagccgcgag gacgaaaggg ccgttctgct caacgtcaac atgcccaaca
     1201 tgcccgtcga ggccctcaag ggcatccgcg gcacccgtct gggcaagcgc aactgcgccg
     1261 agagcgtcat ccgcgagatg agcccgcgcg gctttccgat ctactggctc ggcgctgcgg
     1321 gcaagcccgc cgacgcagag gaggggacgg acttctgggc gacctcccac ggctatgtct
     1381 ccgtgacgcc gctgcaggtc gacctcacga actacaagca ggtcccgacg gtccgaggct
     1441 ggatggacgg ccccgccgtc tgagatccgc aaggcacatt caagggatag aacggaaagg
     1501 aaagaaggtt caaggacatg aacaaaaccg tacgcattct gtcgatctcc gtcgcggccg
     1561 ccgccgcggc cttcctgtcg ggctgctcct ccgtggacat gggcccggct acggtcgtcg
     1621 accactccac ctacagcgac ggcgccgcga ccgcgcccgt ccagacgccg agcctgctgc
     1681 cgggcggcat cgtcgactcc ggccgcaccc acacggtggc cgtgggcgac accatctaca
     1741 acatctccgt gcgctacggc ctctccgccg aacagctcca gcagctcaac gccatcaccg
     1801 atccgacgca gctgcgtctg gggcaggtcc tgaggcttcc cgcctccgtc aacgcgcccc
     1861 gcgcctacac gggcaatccg caggtccgcg tcaaccgtgt gacgaacgac accgtgcttg
     1921 agactgcgcc gatccgcaat gccgagacgg tgcccgctgc gggtgaaacg accgccacct
     1981 cgtcggctgc atccgctgac gccgccgaag ccgcccgcaa gaaggccgag gccgaagctg
     2041 ccgccgagaa ggtccgcacc caggccgaag cggccaagtc ccaggcccag cagaagctca
     2101 acgccgtcac gccgggccag cgcatgatct ggcccgtgag gggcaagatc aagagcgact
     2161 tcgcgaagaa caccttcggt cttgagatcg cgggcacgca gggcgacgtg gtcgtcgcgg
     2221 ccatgcaggg agaagtcatc tgggtgggca cgctcaaggg ctactcgggc acgctcgccg
     2281 tcgtcaagca cagcccgacg ctcatcaccg cctacggcgg ccttgagaac gtggcggtct
     2341 cccagggctc gaagatcaag tcgggccaga agatcgccga cctcaagggc gacacgctga
     2401 ggttcgaggt ccgcgagacc ggcaagtgcg tcgatccgct caagtacctc aacaagcgct
     2461 gatcctcgga gagtcagtca aaaaagagcg ccggcttcac cgccaggcgc tcttcttttt
     2521 ggtaaagtct cctacctgaa tttttgagga cggccggatg aagtcggccg cccagggatc
     2581 cccgttttac atagagagtc aagagtcatg gcacgacgca aagagaaaac ccccgaatac
     2641 ttcaccgtcg aggtggagcg cctcgaccag gaaggccgcg gcgtgggtca tcacgacggc
     2701 aaggtcgtct tcatcgaggg cgcccttccg ggcgagctcg tcacctacga gcgcatccgc
     2761 aacaagacca gcttcgagat cggccgcgtc gcccaggttc accgcgagag cgttcagcgc
     2821 gtcgagcccc gttgccccaa gttcggccac ggtcccggca gctgcggcgg ctgcgccatg
     2881 cagcaccttg agccacgcgc ccaggtcgct tacaaggagc gcacgctcat cgacacgctc
     2941 tggcacatcg gccgcgttcg tcccgaacag atcattacgc cgatctacgg tccgttctgg
     3001 aactaccgtc accgcgcccg cctgacggtc cgcgacgtcg ccaagaaggg cggcgtgctc
     3061 gtcggcttcc atgagaagtc ctcctcctac gtcgccgaaa tgacgggctg ccacatcctc
     3121 acgaagaacg tctccgacat gatcatgccg atgcgcgagc tcgtttccaa gctctctatc
     3181 cgccagcgca tgccgcagat cgaggttgcc gtcggcggca acggacgcac ggccctcgtc
     3241 ttccgcacgc tcgaggagcc gaccgatggg gacatggccc tgctcaacgc cttcggcgcc
     3301 gagcacaagg ccgacatctg gctccagccc aagggtccag actccgccca cccggtcgat
     3361 cccgtcaaca tcaacgcgct cggcctccag cttcccgagt tcggcgttcg catcaacttc
     3421 aagccgaccg acttcacgca ggtcaaccat gagctcaacg agaccatggt gagccgcgcc
     3481 gttcgcctgc tcggcgtgga acccgagcac aaggtcgccg acttcttctg cggtctgggc
     3541 aacttcacgc tcccgctcgc cacgtgcgct tcgcacgtga ccggcatcga gggctccgac
     3601 gcgctcgtcg cccgcgcccg tcagggcgcc gccgacaacg gcctcgccga gaagaccgac
     3661 ttcgtggccc gcaacctctt cacgtggtgc gaccaggact gggaggccct ctggaagaag
     3721 atgggcggca tcgaccgcgt cctcatcgac ccgccgcgcg aaggcgccct ggcgctctcc
     3781 cgcgtgctcg ccgccaccga caagcgtccc gcccgtatcg tctatgtctc ctgcaacccc
     3841 gcgacgctcg cacgcgactg cgccatcctt catcatgagg gcggctggca catccgcgca
     3901 gccggcatca tgaacatgtt cccgcacacg ggccacgtcg agtccatcgc cgtgctcgag
     3961 ccgggtccca ggcccgagaa gaagtccgag gaggccgagg ccatcgagcg ccgcatcgcc
     4021 gcctcgggtg acgacgacgt caagatgaac ctgccgcagt aacctcagac ggctccgtcc
     4081 cgtcccgctg atgcgggctc ggggaacgag actatccaag agtcgaagga gtcgaaaatg
     4141 tcgaaaaaag cgccggacgc catcgcgtcg cggcgttttt ttcgcattcc ggctcatcga
     4201 acacgatgtt ttcct
//
LOCUS       DBMDTI010000171         4177 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_4977_length_4177_cov_2.199215, whole genome shotgun sequence.
ACCESSION   DBMDTI010000171 DBMDTI010000000
VERSION     DBMDTI010000171.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4977_length_4177_cov_2.199215"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="ACZPB6_07785"
     CDS             complement(<1..976)
                     /locus_tag="ACZPB6_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015746.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HIR1184401.1"
                     /translation="MNPTPPQVAGSLNRFLNAIEAAGNKLPHITMLFIWALVITLVIS
                     LALSYVSFDYRHPSTGAVIAVTNMLAPANLLDLVVNSVKNFMGFPPLGITLVATLGIG
                     IAEGSGFIHTLLRKLMSYIPRAALTPAVVIVSILCHVASDSVYVILMPLAALMFYCAG
                     RHPLAGVACSFAGLSGGFTASYTPSVIDPVMQSFTQAAAQTLDASYSVNVLCNYFFAL
                     GGTFFVIAACWYVTDKIVEPRLWATMPLDKGLENDPDLRITPVSALENSAYRKACTVF
                     LGLAALLFVLCWPEGSMLRAPDGSLTSPKAPVMQAIVPLIFIFFSLPGI"
     gene            1154..1663
                     /locus_tag="ACZPB6_07790"
     CDS             1154..1663
                     /locus_tag="ACZPB6_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HIR1184402.1"
                     /translation="MTSASARAWRKRNTIASETRVPQTFFALAAFKPAERLFVEPQAA
                     LTWAWVEGDDFRAGRSFTVHQDDYQSLIGRVGVRACLEFPNEKSTFYARVSGAWEMMG
                     EDEYHAVADAGRVESFRTELDSGWSEYAVGANFKLAPNAFVWADLEKIAGGDIRENYR
                     YTISARRVW"
     gene            1876..2241
                     /locus_tag="ACZPB6_07795"
     CDS             1876..2241
                     /locus_tag="ACZPB6_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184403.1"
                     /translation="MSILRTAAALAAALTLSTAFAAPPPEVVELEGQWRGRLCTAEGN
                     PTKTNLSIDKYGCFVLFQTDVNGAAQSAGTVTVKEGVMTLVDAKTGPYMVLKVSDDGR
                     LVQDVRGKLPKNCCYLKRR"
     gene            2397..3602
                     /gene="hydE"
                     /locus_tag="ACZPB6_07800"
     CDS             2397..3602
                     /gene="hydE"
                     /locus_tag="ACZPB6_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HIR1184404.1"
                     /translation="MSIEQTREILSHEELSDADIVHLLGLTDPEECELLRKTAYDRTT
                     ELMGSFVYYRGLIEFSNICTASCRYCGIRRENHDVERYTMSKEEIVAAAKWAADQGYG
                     SICLQSGERRDEKYIAFVESCLEAIHEATVSEKLPDGVGVTLSLGEQTIETYRRLAKA
                     SGNPSNLRYLARFETSNPELFKVLHGARGDHEKELQNRFRMLRDLREAGYQVGTGVMI
                     GIPGQSLEDLCRDIRTFQQFDVDMIGMGPYLMSEGGELQEYGQMEPKALLQLALNMIA
                     VTRLVLGPVNIAAATALQAIRDDGRELGVSYGANVVMPNLSPQQYREGYQLYDNKPCL
                     DDEPTQCASCLENRLRPIGRSVGWNMSGSSRRWLRRNGRPDEAVPEGLRTKTLEGRRI
                     IPIAAGKVA"
     gene            3895..>4177
                     /locus_tag="ACZPB6_07805"
     CDS             3895..>4177
                     /locus_tag="ACZPB6_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184405.1"
                     /translation="MKSEIRTILLGIGQFLFCALLSASAYGLYVLNLRVSVPVESLIE
                     LGQEALLLSAAIFTLRTASLEKALSGGLWLVGGFLVALYIRELDGWIGHA"
BASE COUNT          821 a         1348 c         1308 g          700 t
ORIGIN      
        1 cgatgccggg gagcgagaag aagatgaaga tgagcggaac gatggcctgc atgacggggg
       61 ccttcgggct cgtgagggag ccgtcaggcg cgcgcagcat ggagccttcg ggccagcaga
      121 gcacgaagag cagggcggcg aggccgagga agaccgtgca ggccttgcgg taggcgctat
      181 tctcaagggc gctcacgggc gtaatcctga ggtcgggatc gttctcgagg cccttgtcga
      241 gcggcatcgt ggcccagagg cgcggctcga cgatcttgtc ggtcacgtac cagcaggcgg
      301 cgatcacgaa gaaggtgccg ccgagcgcga agaagtagtt gcagagcacg ttcaccgagt
      361 aggacgcatc gagcgtctgg gcggcggcct gggtgaagga ctgcatgacg gggtcgatca
      421 cggacggcgt gtaggaggcc gtgaagccgc ccgagagacc ggcgaacgag caggccacgc
      481 ccgcgagcgg atgacggccc gcacaataga acatcagggc tgcaagcggc atgaggatca
      541 cgtacaccga gtcgctcgcc acatggcaga ggatcgagac gatcacgacg gccggcgtga
      601 gcgcggcgcg cgggatgtag ctcatcagct tcctgagaag cgtgtggatg aagcccgatc
      661 cctcggcgat gccgatgccg agcgtcgcca cgagcgtgat gcccagcggc gggaagccca
      721 tgaagttctt cacggaattc acgaccagat caagcaggtt cgcgggggca agcatgttcg
      781 tcacggcgat cacggcaccc gtggacggat gcctgtagtc gaacgagacg taggagagcg
      841 cgagcgagat cacgagcgtg atcacgagcg cccaaatgaa gagcatcgtg atgtgcggca
      901 gcttgttgcc tgccgcctca atggcgttga ggaagcggtt gaggcttcct gcaacttgcg
      961 ggggagtggg attcatttgt agtccttgag gatggaagga cggcctccgt caggcagaga
     1021 ccgaaggctc tattgtacgg aaaatcccga attcggtttt tcggcccaca aagcctgtgc
     1081 gaccgacgtc cccaattgcc agtgatgatg gagatgattc atggccgaca tgccgcaatt
     1141 gcaacggaaa cacatgacgt cagcctcagc gcgagcgtgg cgaaaaagaa atacaatagc
     1201 gtcagaaacg cgtgtccccc agacgttttt tgctctcgcc gctttcaagc ccgccgagcg
     1261 tctcttcgtc gaacctcagg ccgcgctcac ctgggcctgg gtcgaaggcg acgacttccg
     1321 tgcgggccgc agcttcacgg ttcaccagga cgactatcag agcctgatcg gacgcgtggg
     1381 cgtgcgcgcg tgccttgagt tcccgaacga gaagagcacc ttctacgccc gtgtttcggg
     1441 cgcctgggag atgatgggcg aggacgaata ccatgccgtc gccgacgcgg gcagggtcga
     1501 gtccttccgc accgaacttg acagcggttg gagcgagtac gccgtcggcg cgaacttcaa
     1561 gctcgcaccc aacgccttcg tctgggctga ccttgaaaag atcgcgggcg gagacatccg
     1621 tgaaaactac cgctacacca tcagcgcacg ccgggtctgg taaggcgcct gaggggactg
     1681 taatcccttg acgctcgcct gaaacacccc gccggtcctc acgggccggc gggttcgtct
     1741 ttggacgacg gggcttcagc gtcggataat cgaaggcgtg cataatcgat tgaatgatgg
     1801 aggcagtgcc tcccggacat ccgcgtccgg cgcctccagg caagaccaac ccaattgaag
     1861 aggaagaaaa agaagatgtc catcctgcgc accgccgccg cgcttgcagc cgcccttacg
     1921 ctttccaccg ccttcgccgc gccgcctccc gaggtggtcg agcttgaggg ccagtggcgc
     1981 ggccgcctct gcacggccga gggcaatccc acgaagacga atctgagcat cgacaagtac
     2041 ggctgcttcg tgctcttcca gaccgacgtc aacggcgccg cccagtccgc cggcaccgtc
     2101 acggtgaagg aaggcgtcat gaccctcgtc gacgccaaga ccggccccta catggtgctc
     2161 aaggtctccg acgacggccg cctcgtgcag gacgtccgcg gcaagctccc gaagaactgc
     2221 tgctacctca agcgccgctg atccatcagc cccgcctcat tcgaagcccc gtccgctccg
     2281 cgctcggggc ttttgcgctt tcagtgtgcg gcgcacgcgc cgccccctcc atcgggagta
     2341 tttcgccagc cgtcccgatc gtgcataatg tgcctctcat actaggagca accccaatgt
     2401 ccatcgagca gacgcgtgaa atcctctccc atgaagaact gtcggacgcc gacatcgttc
     2461 atcttctcgg cctcacggat cccgaggagt gcgagctcct gcgcaagacc gcctacgaca
     2521 ggacgacgga gctgatgggc agcttcgtgt actaccgcgg cctcatcgag ttttcgaaca
     2581 tctgcacggc gagctgtcgc tactgcggca ttcgtcgcga gaaccacgac gtcgaacgct
     2641 acacgatgtc gaaggaggag atcgtcgcgg ccgcgaaatg ggccgccgat cagggctacg
     2701 gctcgatctg ccttcagtcg ggcgagcgcc gcgacgagaa gtacatcgcc ttcgtcgaat
     2761 cgtgccttga ggcgatccac gaggccacgg tctcggagaa gctccccgac ggcgtgggcg
     2821 tgacgctctc gctcggcgag cagacgatcg agacctaccg ccgtctcgca aaggcctcgg
     2881 gcaatccctc gaatctcagg tacctcgcca gattcgagac ctcgaacccc gagctcttca
     2941 aggtccttca cggcgcccgc ggcgaccatg agaaggagct tcagaaccgc ttccgcatgc
     3001 ttcgcgacct tcgcgaggcg ggctatcagg tgggtacggg cgtcatgatc ggcattcccg
     3061 gccagtcgct cgaggacctc tgccgcgaca tccgcacctt ccagcagttc gacgtcgaca
     3121 tgatcggcat gggcccctac ctcatgagcg agggcgggga gcttcaggaa tacggccaga
     3181 tggagcccaa ggcgctcctt cagcttgccc tcaacatgat cgccgtcacg cgcctcgtgc
     3241 taggccccgt caacatcgcc gccgccacgg cccttcaggc catccgcgac gacggccgcg
     3301 agctcggcgt ctcctacggc gccaacgtcg tgatgcccaa cctctcgcca cagcagtacc
     3361 gcgagggcta ccagctctac gacaacaagc cctgccttga cgacgagccc acccagtgcg
     3421 cctcctgcct cgaaaaccgc ctgcgcccca tcggccgcag cgtcggctgg aacatgagcg
     3481 gaagctcccg caggtggctg cgccgcaacg gccgccccga cgaggccgta cccgagggcc
     3541 tgcgtaccaa gaccctcgaa ggccgcagga tcatccccat cgctgcggga aaagttgcgt
     3601 gaaaacaaca cctgcccgaa atcccgccgc ggctccccgt gtgcccggcc tccggcaaag
     3661 ccccgccccg agcggggctt ttttcacccc tacgttcctc atccacaggg cattttcagt
     3721 ccgcccgtgc agcagtcgaa agcagggcgt cccgctgggc gagaaaactt tgcagacggg
     3781 gagagcggga atgagcgaac gggtattgac gttttcgcgc cgccaggtag aatggacggt
     3841 ttcccaaaaa ctcgggttaa cccgagcaag acccaagaat caaatcccgc cctcatgaag
     3901 tccgaaatcc gcacgatcct gcttgggatc ggtcagtttc ttttctgtgc gttgctgagc
     3961 gcgtccgcct acggcctcta cgtcctgaac ctcagggtaa gcgtccccgt cgagagtctg
     4021 atcgagctgg gccaggaagc gctgctcctt tcggcggcga tcttcacgct caggaccgcg
     4081 tcgcttgaga aggcgctctc gggcgggctg tggctcgtcg gcggctttct cgtcgcgctc
     4141 tacatccggg agctcgacgg ttggatcggc cacgcct
//
LOCUS       DBMDTI010000172         4091 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5105_length_4091_cov_1.934837, whole genome shotgun sequence.
ACCESSION   DBMDTI010000172 DBMDTI010000000
VERSION     DBMDTI010000172.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5105_length_4091_cov_1.934837"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="ACZPB6_07810"
     CDS             complement(<1..361)
                     /locus_tag="ACZPB6_07810"
                     /inference="COORDINATES: protein motif:HMM:NF015999.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR1184406.1"
                     /translation="MSHDAACADLIRKGFGTRIITRETTIATVEEAAATVGVGVERIA
                     KTLSFLQNGRPVLIVAAGNVKIDNRKYKDRFGVKASMIPGASVEELVGHAPGGVCPFG
                     IREGVEVWLDASLKLYET"
     gene            complement(575..1849)
                     /locus_tag="ACZPB6_07815"
     CDS             complement(575..1849)
                     /locus_tag="ACZPB6_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR1184407.1"
                     /translation="MSALIRIAAFLATAVLSPAALAAEASMDKADVVWMLVSTTLVLF
                     MTIPGIALFYGGMARKKNVLSILAQTTVICCALTLVWFAAGYSLAFGPGSAFVGGLSH
                     VFLNGLDINSMSGSIPTLLFVCFQMTFAILTATLMIGAFAGRMKFSAMLVFMILWSLC
                     VYSPVCHWVWAEGGFFFDMGALDYAGGTVVHINAGVSGLVACLVLGKRLGYGREPMSP
                     HNLTFAMLGAAILWIGWFGFNGGSGLAANERAVMAILVTQIAAAAAGVSWMFVEWMLR
                     GRPSLLGLISGAVGGLVVITPASGFVGPVAALVMGLIGGVCCFWGAVFLKRMAGWDDS
                     LDAFGVHGVGGIVGALLTGVFASSAVTGSEMNPVMTQVGIQTASVVATMVYAGAVSWI
                     LLKIIDVVMGLRVTSDEERMGLDLSLHGERIE"
     gene            complement(1909..2247)
                     /locus_tag="ACZPB6_07820"
     CDS             complement(1909..2247)
                     /locus_tag="ACZPB6_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR1184408.1"
                     /translation="MKYIIAVIKPFKLDDVREALADCGVSGLTATEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIKLEIAVSDELCDKVVESIASAANTGRIGDGKIFVMDLEHVMRI
                     RTGETDDAAV"
     gene            complement(2614..3537)
                     /locus_tag="ACZPB6_07825"
     CDS             complement(2614..3537)
                     /locus_tag="ACZPB6_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1184409.1"
                     /translation="MEDSFAKVMSLAVFIVLGYLLRRFNILKDQAFAAISALVMNVTL
                     PCVILTNLNGVRIEGDMLLIAGLGLLTNIIFLVWGLFLSRNIADTQWRDFVRLNVGGY
                     SVGPFAVPYVQSFFPTTGLMATCMFDVGNCVMAGGGTFAVIAGTRVKTTLWRTVKVIA
                     SKLVRSGPLVTFAFVGLMSVLDMRLPEGVITSTSVGAHANTFLCMIMIGESISFSMGG
                     GKMGKVLKLLASRWVFCILIALGVWHFLPFEEEIRMALTLTCLSPIPAMSLVFTAQLD
                     GDIAMAANMSSLSVGCSIIGMSVALMLFGAL"
BASE COUNT          847 a         1219 c         1343 g          682 t
ORIGIN      
        1 cggtctcgta gagcttcagg gacgcgtcca gccagacttc gacgccctcg cggatgccga
       61 agggacagac gccgccgggc gcatgtccca cgagttcctc gaccgaagcg cccggaatca
      121 tcgaggcctt cacgccgaag cggtccttgt acttgcggtt gtcgatcttc acgttgcccg
      181 cagccacgat gagcacaggc ctgccgttct gaagaaagga gagcgtcttg gcgatgcgct
      241 ccacgccaac gcccacggtg gcggcagcct cctcgacggt ggcaatcgtc gtttcccttg
      301 tgatgatgcg ggtgccgaat ccctttctga tcaagtcggc gcaggcggcg tcgtgcgaca
      361 tgaaggctcc tccgtggaaa cgcaagcaga tcattcgatc ataggcgctc gagcgtccgc
      421 gcgcctcagg cgtccgcctt atcctgagcg gcctgcatca agcatcgtca cgagaattcc
      481 ttcgttatcc gcgcgaacaa atgaaggggc cgcccggcct ttgcgtcttc acgcggcggc
      541 gggcggccca ttcgggttca ggtgaatgtc gggatcattc gatgcgttca ccgtggagcg
      601 agaggtcgag acccatgcgt tcttcgtcgg acgtgacgcg caggcccatg acgacgtcga
      661 tgatcttgag gaggatccag ctcacggcac ccgcgtagac catcgtggcg acgacgcttg
      721 cggtctggat gccgacctgg gtcatgacgg gattcatttc ggaacccgtg acggcgctcg
      781 aggcgaagac gccggtgagg agggcgccga cgatgccgcc cacgccgtgc acgccgaatg
      841 catcgagcga gtcgtcccag cccgccatgc gcttgaggaa gaccgcaccc cagaagcaac
      901 agacgccgcc gatgaggccc atcacgagtg cggcgacggg accgacgaag cccgaggcgg
      961 gcgtgatgac cacgaggccg ccgacggcgc ccgaaatcag gcccagaagc gagggacggc
     1021 cgcgaagcat ccattcgacg aacatccagg agacgcccgc tgcggcggcc gcgatctgcg
     1081 tgacgaggat cgccatgacg gcgcgctcgt tggcggcaag gccggagccg ccgttgaaac
     1141 cgaaccagcc gatccagagg atggcggcgc cgagcatcgc aaaggtgagg ttgtgcggac
     1201 tcatgggctc gcgaccgtag ccgaggcgct tgcccagcac gagacaggcg acgaggcccg
     1261 agacgcccgc gttgatgtgc acgaccgtgc cgcccgcata gtcaagcgcg cccatgtcga
     1321 agaagaatcc gccttcggcc caaacccagt ggcacacggg cgaatagacg cacagcgacc
     1381 agagaatcat gaagaccagc atcgccgaga acttcatgcg gcccgcaaag gcgccgatca
     1441 tcagcgtagc cgtgagaatc gcgaacgtca tctggaagca gacgaagagc aaagtcggaa
     1501 tggagcccga catgctgttg atgtcgagac cattgagaaa cacatgcgag agcccgccca
     1561 cgaaggcgct gcccggcccg aaggcgagcg aatagcctgc ggcgaaccag acgagcgtga
     1621 gcgcgcagca gatgacggtc gtctgcgcaa ggatcgagag cacgttcttc tttctggcca
     1681 tgccgccgta aaagagtgcg attcccggga tcgtcatgaa gaggacgagc gtcgtggaga
     1741 cgagcatcca gacgacgtcg gccttgtcca tggaggcctc ggcggcaagc gccgcgggtg
     1801 aaaggaccgc cgtagcgagg aatgcggcga ttcggatgag tgcggacatt tgtcttttct
     1861 cctgaatgtt cggtcttctt taaatcaaat cgagtgctgc cgaccgcctc agacggccgc
     1921 gtcgtccgtt tcgcccgtcc tgatgcgcat gacgtgctcg aggtccatca cgaagatctt
     1981 cccgtcgccg atcctgccgg tgttcgcggc cgaggcgatc gactcgacca ccttgtcgca
     2041 gagctcgtcg gaaacggcga tctcgagctt gatcttcggg agaaagtcaa cgacgtattc
     2101 ggcgccgcgg tagagctccg tgtgtccctt ctgacgcccg aagcccttga cttccgtggc
     2161 ggtgagcccg ctgacgccgc agtccgcgag cgcttcgcgc acgtcgtcga gcttgaacgg
     2221 cttgatgacc gcaatgatgt acttcatgat ttttcctctg tgagtgaaat gctttgtgcg
     2281 gatggcgggg attcccgcgc ccttgatcca ctcttaagca aaaggcgtgc caaaaccccg
     2341 tattcgccta taggagacga aattcggcaa atggaacctc aacggggcca gaaacgggtg
     2401 gaaattcgat taaagacggc ggcagaggcg tgtttaagcg attcggggtg tcggcatgca
     2461 ccgttttgag gcacggcgcg tgcgcaggcg cccgcgagtg gcgccgtgcg gccgggcggg
     2521 cacggagaac ggggaggaaa aggggagata tgaacgaaat cgggcggccc tccggagagg
     2581 accgcccgaa cgtggtgcgg aggttctgat gcgtcagagc gcgccgaaga gcatgagcgc
     2641 gacggacatg ccgatgatgg agcagccgac gctcagcgaa ctcatgttgg cggccatggc
     2701 gatgtcgccg tcgagctgtg cggtgaagac gaggctcatg gcgggaatcg gagagaggca
     2761 ggtgagggtg agcgccatgc ggatctcctc ttcgaaggga aggaagtgcc agacgccgag
     2821 cgcgatgagg atgcagaaga cccagcggct cgcaagcagc ttgagcactt tgcccatctt
     2881 gccgccgccc atcgagaagc tgatcgactc gccgatcatg atcatgcaga ggaaggtgtt
     2941 ggcgtgcgcg ccgacggagg tgctggtgat gacgccctcg ggaaggcgca tgtcgaggac
     3001 ggacatgagg cccacgaagg cgaaggtgac gagcgggccc gagcgcacga gcttcgaggc
     3061 gatcaccttg accgtgcgcc agagcgtcgt cttcacgcgg gtgcccgcaa tcaccgcaaa
     3121 ggtgccgccg cccgccatga cgcagttgcc cacgtcgaac atgcaggtcg ccatcaggcc
     3181 cgtcgtcggg aagaaggact gcacgtacgg aaccgcaaac ggaccgaccg aatagccgcc
     3241 cacgttgagc ctcacgaagt cgcgccactg cgtgtccgca atgttgcgcg aaagaaaaag
     3301 cccccagacg aggaagatga tgttcgtgag gagcccgagg cccgcaatga ggagcatgtc
     3361 tccttcgatc ctgacgccgt tgaggttcgt gaggatcacg cacgggagcg tgacgttcat
     3421 caccagggcg ctgatggccg caaaggcctg gtccttcagg atgttgaagc gcctgagcag
     3481 atacccgagc acgatgaata ccgccagaga catcaccttg gcgaagctgt cttccatgat
     3541 tgccttccgt ctttttgttg aggggccggc gcccgtgcgt tcgggcgccc gaacaggaaa
     3601 ttgtgcgcct gcagaggccg aaattctacc tcccgaggta tgtatcggca ctacgccgaa
     3661 agaccgaggc gggttgatcg ggaaagcctc tgcaacggaa tccagcggat gcggggatgg
     3721 tgccgggaga ggctgcacga aggcgcgaaa tcgcgcgaac aacaaccaca cgaccgttac
     3781 gaccaagact gctgaagtag gtgccagcct cgtccacgtg ttctgatcat caggcttcgg
     3841 cctctgacgg ccgcaccgcc tgaatgaaga gggcgcccgt ctgggaatat tccgggcagg
     3901 cgcccttcgc tttttcggac gaaaaggacg ctgccggctc atgacacgga ggctatcctc
     3961 ctcaatcact cgccgcgtga gaaaggtgag gggagcggct tgcacgccaa ctgccccttc
     4021 gatatactca cgtccttttt cggcgccttc acgggcgccg tttgttgttg tgaaaagcct
     4081 gttgggcaga a
//
LOCUS       DBMDTI010000173         4081 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5123_length_4081_cov_3.044472, whole genome shotgun sequence.
ACCESSION   DBMDTI010000173 DBMDTI010000000
VERSION     DBMDTI010000173.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5123_length_4081_cov_3.044472"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="ACZPB6_07830"
     CDS             <1..533
                     /locus_tag="ACZPB6_07830"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HIR1184410.1"
                     /translation="GLRGDEPWRPISVFRTYESHIMQVRPWLPQAIGCLIHLAFGMAD
                     LSVYLPFYQGLEAVPQHYGCGTDHCDRRSVYWKFRRLQTLVMTDYAGLAPMVKTAFAK
                     FEADLAPRMAAFEAEFVKLHAEDEKAAAKLLNDFNLRVLAEGEELCETLLDDCFTKRT
                     TDIQEAVYFRNRKAKD"
     gene            complement(726..1631)
                     /locus_tag="ACZPB6_07835"
     CDS             complement(726..1631)
                     /locus_tag="ACZPB6_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1184411.1"
                     /translation="MTSVRRMLTGFLLAIAAGFGWGSMGVAAQVMMTDGGFTAQDLVA
                     LRVFGAGVLLTLMTLLTERGRVSKLFSDMVLLRDVVIYGLGVLATQFTFFLAIRDAGA
                     ATAAIMVITGPLFVIGWIAATTRKPVSILELSAFCLAATGVVLIITKGDLSVVNFSYS
                     GVLWGLVSAACGAFCTLQPGNAVKKLGVNAVVGFGMLFAGIGALFVYPPTEIDAVWTW
                     ETLAGYLYIIVVGTALAFVCYLKSLDYVPPAVTTILGASEPLTAVILSVVLLGTSFTP
                     VESLGAVLIFATVFILAMREGRRRA"
     gene            1863..2048
                     /locus_tag="ACZPB6_07840"
     CDS             1863..2048
                     /locus_tag="ACZPB6_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184412.1"
                     /translation="MIRIETDNTVKDGCRVRQLRACLKGRDMRERLYSPDRLKYPVNN
                     SRLKSKALLSPAKAGST"
     gene            complement(2056..3252)
                     /locus_tag="ACZPB6_07845"
     CDS             complement(2056..3252)
                     /locus_tag="ACZPB6_07845"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HIR1184413.1"
                     /translation="MRTVCIALEVDADASEKMHQTRRLYGQACNLLVPIVVSDTDRKK
                     RLWQRYNLHKAAYPMVRTKMSVLGAQLACNVMRSVSSMYQSWISNHPNFSKDKKMVLP
                     SISFRNPVVHLDKNTIRFFNNYTEASVYTVNGRVGVRLRPGKFQLSQLAGFLAEELAG
                     TSKENRIYRLGECNLVWKCGIKGTPSRWELHIAIEKKLEEVSLDNLSLNQVMGVDVGE
                     NNAAALSTGRIFKAGKMKDDRDKYLSSRARFQRNGSRSARQALRKASGREKRHVEQIN
                     DEISKSIIEDAAERNIRLIVLEDLTDIRERIKAGQKVRARLHRWPFRELQQKIVDKAC
                     RAGIEVIFVAPRYTSQTCPHCNAIGTRQKHRFVCKNCGYRAHSDLNGGRNLQRLGCLL
                     ITQGLV"
     gene            complement(3275..3697)
                     /gene="tnpA"
                     /locus_tag="ACZPB6_07850"
     CDS             complement(3275..3697)
                     /gene="tnpA"
                     /locus_tag="ACZPB6_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HIR1184414.1"
                     /translation="MGVLKSFLYNRNCVYQTAYHVIWCPKYRKRILKGEIAEALRLAI
                     TAICIEREWPILTLEIQPDHIHLFVTIPPSQAVADAVKILKGSTARKLFVAFPALKDQ
                     LYGGHLWSPSYYVGTAGNVSAETIRHYIERTEHIKGRR"
BASE COUNT          888 a         1182 c         1169 g          842 t
ORIGIN      
        1 tcggtctgcg cggcgacgag ccctggcgcc cgatcagcgt gttccgcacg tacgagtccc
       61 acatcatgca ggtccgtccg tggctcccgc aggccatcgg ctgcctgatc cacctcgcct
      121 tcggcatggc cgacctctcc gtctacctgc cgttctacca gggcctcgag gccgttcccc
      181 aacactacgg ttgcggcacg gaccactgcg accgccgttc cgtctactgg aagttccgca
      241 ggcttcagac gctcgtcatg accgactacg ccggcctcgc cccgatggtc aagaccgcct
      301 tcgccaagtt cgaggccgac ctcgccccgc gcatggccgc cttcgaagcc gagttcgtca
      361 agcttcatgc cgaagacgaa aaggccgccg cgaagctcct caacgacttc aatctccgtg
      421 ttctcgccga aggcgaagag ctctgcgaaa cgcttctcga cgactgcttc acgaagcgca
      481 cgaccgacat ccaggaggcc gtgtacttcc gcaaccgcaa ggccaaggac tgatcaccgg
      541 gcttgccggc ctctttccgc ctttcttgtc atgagggcgg gatgaatgcc cggcctgcct
      601 cacttgaaaa tggcgggatt tccataacgg aagtcccgcc attttttgct tctgtcggtt
      661 ctgtctcagt cgccgggttt cgcttgagcg ggagcaaatg agcggtgttg aagacaggat
      721 cgggatcagg ccctgcgtct cccctccctc atggcgagga tgaagacggt ggcgaagatg
      781 aggactgcgc cgaggctctc gacgggcgtg aaggaggtgc cgagcagcac gacggagaga
      841 atcacggccg tgaggggctc ggaagcgccg aggatggtgg tgacggcggg cgggacgtag
      901 tcgaggctct tcagatagca gacgaaggcg agcgcggtgc ccacgacgat gatgtagagg
      961 tagcccgcga gggtctccca agtccagacg gcgtcgattt ccgtgggcgg atagacgaag
     1021 agcgcgccga tgcccgcaaa gagcatgccg aagcccacga cggcgttgac gccgagtttc
     1081 ttgacggcat tgcccggctg caacgtgcag aaggcgccgc aggcggcgga gacaagcccc
     1141 cagaggacgc ccgaatatga gaagttgacg acgctcaggt cgccctttgt gatgatgagt
     1201 acgacgcccg tggcggcaag gcagaaggcg gagagctcga ggatcgagac gggcttgcgg
     1261 gtcgtggcgg cgatccagcc tatcacgaag agcggacccg tgatgaccat gatggccgcc
     1321 gtggcggcgc ccgcatcacg gatcgcaagg aagaacgtga actgcgtcgc cagcacgccg
     1381 aggccgtaga tcacgacgtc acgcagcaga accatgtcgg agaagagctt cgagaccctg
     1441 cccctttcgg tgaggagcgt catgagcgtg aggagcacgc ccgcgccgaa gacgcgaagc
     1501 gccacgaggt cctgggcggt gaagccgccg tccgtcatca tgacctgagc agccaccccc
     1561 atggagcccc agccgaagcc tgcggcgatg gcaagaagaa agcccgtgag catgcggcgt
     1621 actgaggtca tttgtcttgt cttttccttg gagtgcgtgt gagaaggcgg tccgtccgga
     1681 ccaaggcggc attgtacagc gtcgcgatgc accgccgctc gaaacgcccc gtccatcgtc
     1741 atcctcaagc ctgcgggcgg taaagacggc ggcctgcgct tgcctcccgt caagaaatcc
     1801 tccaaaagac gctggagcgt tgttcctcat cgaaatggcg cgcgtacacg aagaacggca
     1861 ccgtgatccg catcgaaacc gacaacaccg tgaaggacgg ctgcagggtg cgtcagttgc
     1921 gcgcctgcct caagggccgc gacatgcgcg agcgcctcta cagtcccgat cgactgaagt
     1981 atccggtaaa caactctcga ctaaagtcga aagctttatt gtcacctgct aaagcaggct
     2041 caacataggg cttgtttaca ccagcccctg ggttatcagc agacaaccca gacgttgcag
     2101 attccgacct ccattgaggt cgctgtgcgc tctgtaacca cagttcttgc aaacaaatcg
     2161 gtgcttctgc cgtgttccga tggcattgca atgcggacag gtttgcgacg tatagcgagg
     2221 ggcaacgaag atcacttcga tacccgctct acaagctttg tccactatct tctgctgaag
     2281 ctccctgaag ggccagcggt gcaggcgtgc gcgaaccttc tgcccggcct tgattcgctc
     2341 gcggatgtcc gtaagatctt caagaacgat caggcgaata ttgcgttctg ccgcatcttc
     2401 aataatggac ttgctgattt cgtcgttgat ttgttccaca tgtcgtttct ctcggccgga
     2461 agcctttcga agtgcctgtc tggcgcttct ggagccattg cgctggaaac gagcccgact
     2521 gctcaggtat ttgtcacggt cgtccttcat cttcccggct ttgaagattc ggccggtaga
     2581 cagtgcggcg gcgttgtttt cgccaacatc aacgcccatg acttgattca aagacaagtt
     2641 gtcgagagat acttcctcga gtttcttctc tatggcaatg tgcagctccc atctggaagg
     2701 cgtgcctttg atgccgcact tccaaacgag gttgcattcg ccaagcctgt aaatccgatt
     2761 ctccttcgac gttccggcaa gctcttcagc cagaaaaccg gccaattggc tcagctgaaa
     2821 ctttcccgga cgaagacgaa cgccgacacg cccattcacc gtatagaccg acgcctcggt
     2881 gtagttgttg aagaagcgaa tggtgttctt gtccaggtga acaacaggat tcctgaagct
     2941 gatgctcggg agcaccatct tcttatcctt cgagaagttc ggatggttgc tgatccacga
     3001 ctggtacatg ctcgataccg aacgcatgac gttgcaggcg agctgtgcac caagaacgga
     3061 cattttggtt cgaaccatcg gataagcagc cttgtgcagg ttgtaccgtt gccagagccg
     3121 ttttttccta tctgtatcgg acacgacgat tgggacgagc aaattgcagg cctgcccgta
     3181 gagacgacgg gtctgatgca tcttttcgga agcatcggca tcgacctcca aggcaatgca
     3241 gacggtacgc acgtcgactt tgcgtcgaac gagcttacct gcgccctttg atatgttcag
     3301 tcctttcaat gtaatgcctg atggtttccg cactgacatt gccagccgta cctacgtaat
     3361 aggacggaga ccacaaatgc ccgccgtaaa gctgatcctt caacgccggg aaagccacaa
     3421 acagcttccg ggccgtcgaa cccttcaaaa tctttacggc atcggctaca gcctgtgacg
     3481 gagggatggt tacgaacaaa tggatatggt caggttggat ttccaaggtc agaatcggcc
     3541 actcgcgctc aatgcagatc gcggtgatgg caagacgcaa agcctcggct atttcacctt
     3601 taagtatccg tttgcggtac ttcggacacc atatcacatg gtatgccgtc tggtaaacgc
     3661 aatttcggtt gtataggaag ctcttcaaaa cccccatctc tgaacgatgg gggaagcaga
     3721 tttgtttaaa cgagtgactt ttggccaggt gttgaaaagc gtcgaaagtt cccgtgaacg
     3781 ttagtcgttc gatttttccg aataatgctg aaacaactcg atatttgcct aaaaattggg
     3841 caaatatcgt tttcaagagt cggcaaggcc ccatggcact cgagacgaag cgcgcaccag
     3901 agtttcgggg taatcagcaa ggtagccttg gcggagcgca ccatttgcgc aagcggttgc
     3961 agtcgtagag agcaaacaag gtgagcgctc gactgtgatt tttcgccaac ccgcgataac
     4021 gaaccttggc atagccataa atcctcttca gccagaggaa gcaccactca atcgagcatc
     4081 g
//
LOCUS       DBMDTI010000174         4081 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5124_length_4081_cov_2.023116, whole genome shotgun sequence.
ACCESSION   DBMDTI010000174 DBMDTI010000000
VERSION     DBMDTI010000174.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5124_length_4081_cov_2.023116"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="ACZPB6_07855"
     CDS             <1..539
                     /locus_tag="ACZPB6_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR1184415.1"
                     /translation="EKRFAILAELVSSLKDLPENRRYRDTVLDVRRHVDFIGEEVDDD
                     GRQIEVYRSGAGKSGGQRQKLTTTCLAAALRYQLCGDSVSVPTYALVVLDEAFDKADS
                     DFTTLAMNIFRQFGFQMIVATPDKAVTTLEPFIGGACVVHIVERKYSSVLTIPYDGAV
                     HRLVWKAPAESAEPVKEA"
     gene            539..1750
                     /locus_tag="ACZPB6_07860"
     CDS             539..1750
                     /locus_tag="ACZPB6_07860"
                     /inference="COORDINATES: protein motif:HMM:NF023223.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetD
                     domain-containing protein"
                     /protein_id="HIR1184416.1"
                     /translation="MKTPAEVRTTAARRFRASCRRWLAGHLGLPDDSAWPFAVPLGVP
                     SEREVLADPQKTLDWRDAWSAEAESGRLKVAFAVRDWTACGRQTVPERAVFESPEAVA
                     RAAGELHTWRRALERASRLVDGEDGLREALVRAWSVLAEWEDEDFAILLSAFRWLADN
                     PESGLYVRQLPVEGMDTKWLTKPRMTVLRDLLAARWRASGRPVPEAQAFEAFAGLRSR
                     PSSVRMRLLDPADRALMHGLSDLSAPPEEIAILPLHPRIVFISENEQSGLAFGDVPGA
                     VLFFGLGKGVTQLDAVPWIHEGTVIYWGDADTWGLQILASLRRRFPKTRAVMMDEATL
                     VRFHAYAVEEPRQAPEAVCEGLLPAEAALWDALKSDKYGMRLRLEQERIPWAHAWAEL
                     MKAVAEASRKS"
     gene            complement(1878..2642)
                     /locus_tag="ACZPB6_07865"
     CDS             complement(1878..2642)
                     /locus_tag="ACZPB6_07865"
                     /inference="COORDINATES: protein motif:HMM:NF015057.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HIR1184417.1"
                     /translation="MAHNLLTRRRLIGAAGLGMAGIVSGPLRAAVKPAFRWQDEAAGA
                     AAPIAAACVNHPFVRGLADGSLSEERFLYYLGQNIHYLVNYRRALSALAKRLKNPAHR
                     RQFEAWAEETAATERYTRDCFIRANPKQLDVLPISPTTQLYMGWEAQAASFLPVHEAV
                     GAMLPCFWSYGEVGRFVARTKKTEDNPYAEWIAGYGDPAYAGTVDRAMGIGSEIAETL
                     KTEERLAMTAAFLRSNRLEWMFWDAAWRLEAWPPIH"
     gene            3108..3869
                     /locus_tag="ACZPB6_07870"
     CDS             3108..3869
                     /locus_tag="ACZPB6_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR1184418.1"
                     /translation="MLPDLTTLLIVCPLVFLGGLVDSIAGGGGLITLPGYLLAGVPPH
                     MAIGTNKLSSAMGMAISTVRLYRGGFIDLKMSVVPVLSAFVGSLVGARIALMVPPSVF
                     QIILVLCLPVAAFLVMRDKTLSPDGEEMEQKKRWLMLGLVSFACGAYDGFYGPGAGTF
                     MLLGFAAAAKLGVRDAAGQMKIVNFSSGIAALVLYAATGHVDWVLGALAGCFGITGHY
                     IGSGLVMKNGGRIVRPIILTVLAILFVKTAWDYLA"
BASE COUNT          704 a         1341 c         1245 g          791 t
ORIGIN      
        1 ctgaaaagcg cttcgcgatc ctcgccgagc tcgtgtcgag tctcaaggac ctgcctgaaa
       61 accgccgcta ccgcgacaca gtgcttgacg tacgccgcca cgtcgacttc attggcgagg
      121 aagtggatga cgacggacgc cagatcgagg tctaccgcag cggcgccggc aagtccggcg
      181 gccagcgcca gaagctcacg acgacttgcc tggctgccgc cctcaggtac cagctctgcg
      241 gcgacagcgt gtcggtcccg acctatgcgc tcgtggtgct cgacgaggcc ttcgacaagg
      301 ccgacagcga cttcacgacg ctcgccatga acatcttccg ccagttcggc ttccagatga
      361 tcgtcgccac gcccgacaag gccgtcacga cgctcgagcc cttcattggt ggcgcctgcg
      421 tggtgcacat cgtcgagcgc aagtactcga gcgtgcttac gatcccctac gacggcgcgg
      481 tccaccgcct cgtctggaag gcgcccgctg aatccgcaga acccgtgaag gaggcctgat
      541 gaagacgccg gccgaggtcc gcacgactgc ggcgcgtcga ttccgggcgt cgtgccgcag
      601 gtggctcgcc ggtcatctgg gccttcccga cgactcggcc tggccgtttg ccgtcccgct
      661 tggcgttcct tccgagcgcg aggtcctcgc cgacccgcag aagacgctcg actggcgcga
      721 cgcatggagc gcggaggccg aatcgggccg tctcaaggtg gccttcgcgg tgcgggactg
      781 gacggcctgc ggccgtcaga ccgttcccga acgtgcggtg ttcgagtcgc ccgaagccgt
      841 tgcgcgtgca gcgggcgagc ttcacacatg gagacgtgcg ctcgagcgtg cctcccgcct
      901 cgtcgacggc gaagacggcc tgagggaggc gctcgtgcgt gcctggagcg tgctcgccga
      961 atgggaggac gaggatttcg cgattctgct ctccgccttc aggtggcttg ccgacaatcc
     1021 cgaaagcggt ctctatgttc gtcagctgcc cgtcgagggc atggatacca agtggctcac
     1081 gaagcccaga atgacggtgc ttcgcgatct tctcgccgcc cgctggcgtg cttccgggag
     1141 gcccgtgccc gaggcgcagg ccttcgaggc cttcgcaggt ctgaggagca ggccctcgag
     1201 cgtgcgcatg aggcttcttg atcccgcaga ccgtgcgctc atgcacggac tctccgacct
     1261 ctcagcgccg ccggaggaaa tcgcgatcct gccgcttcat ccgagaatcg tcttcatttc
     1321 cgaaaacgag cagtcgggcc ttgcgttcgg cgacgtgccg ggcgccgtcc tctttttcgg
     1381 tttgggcaag ggcgtcacgc agctcgatgc cgttccctgg attcatgagg ggacggtcat
     1441 ctactggggc gatgccgaca cgtggggcct tcagattctc gcgtctttaa gacggcgctt
     1501 tcccaagacg cgcgccgtca tgatggacga ggcgacgctc gtgcgctttc atgcctacgc
     1561 cgtcgaggag cccaggcagg cgcccgaagc tgtctgcgag ggccttctgc ctgctgaagc
     1621 cgcgctttgg gacgcactca agtccgacaa atatggcatg cgccttcgcc ttgagcagga
     1681 gcgcatcccc tgggcccatg cctgggccga actcatgaag gcagttgccg aagcttccag
     1741 aaagtcctga gtctgaggtc taatctaaac ggcacatgca ccctcatgct ccgtcgagtg
     1801 cgagcgtttg cgcatcgagc gctacggttc gacctatgat gatccgtaag ctctccatgg
     1861 ggtggaacgc ccgcatctca gtggatcggt ggccaggctt ccaggcgcca ggcggcgtcc
     1921 cagaacatcc attcgaggcg gttcgaccgc aggaatgcgg ccgtcatggc taagcgctct
     1981 tcggtcttga gcgtttcggc gatctcgctt ccaattccca tcgcgcgatc cacagtaccg
     2041 gcatatgccg ggtcgccgta gccggcgatc cactcggcat acggattgtc ctcggtcttc
     2101 ttcgttcgcg cgacaaagcg gccgacctca ccgtagctcc aaaagcacgg cagcatcgca
     2161 ccgactgcct catgcacggg cagaaagctt gcggcctgtg cctcccagcc catgtagagt
     2221 tgggtcgttg gcgaaatcgg aagcacgtcg agctgcttag ggttcgcgcg tatgaagcag
     2281 tcgcgcgtat agcgctccgt cgctgccgtc tcttcggccc atgcctcgaa ctggcggcga
     2341 tgggcgggat tcttcaggcg ctttgcgagc gccgagagag cgcggcgata gttgacgaga
     2401 taatggatgt tctgtccgag ataatagaga aagcgctcct cggagagcga cccatcggcg
     2461 agtcccctca cgaatggatg gtttacacag gcggccgcaa tcggcgccgc cgcgcctgcc
     2521 gcctcgtcct gccagcgaaa tgcgggcttg acggccgctc gaagcggccc ggacacgatc
     2581 ccggccatgc cgaggcccgc cgcgccaatg agtcggcggc gagtcaaaag attatgcgcc
     2641 atcaatcatc ctcaacttcg cacgggtttc tatcacttcg agcacatcga tgacggtgcc
     2701 gtcggccatc gccatggcca tgttcccgaa acccagaaaa gcaaaccgag ccgtagacaa
     2761 atccagcatc cgaattccct ccttttgctt ggtcgtttcg aaacacttgt cccacgctca
     2821 aaatgggaat tatactcagg ccacggcgtc aggccacggc gcgctgccgc tttcaaggcg
     2881 cacgcgtttc gcctgggatg tcaggccatg cacatgcgga actttgccga gcggccctcc
     2941 gagggcgcta gggcgcgtgt cgcggcctga attcaacgag tgatgcatgg caggggaatt
     3001 cgcgaaaaag tcgcggatct gatcatgagg gtcgcgcggg cagagtagaa tttcgcatcc
     3061 atttcgaaat cgccaccaaa aacggacctc atattcaggc tcaggccatg cttcccgatc
     3121 tcaccacact gctcatcgtc tgcccgctcg tctttctggg cggcctcgtc gactccattg
     3181 cgggaggcgg cggactcatc acgctgccgg gctatctgct cgcgggcgtt ccgccccaca
     3241 tggccatcgg cacgaacaag ctttcaagcg ccatgggcat ggccatctcg accgtgcgcc
     3301 tctaccgagg cggcttcatc gacctgaaga tgtccgtggt ccccgtcctc tccgccttcg
     3361 tgggcagcct cgtgggcgcg cgcatcgcgc tcatggttcc gccctcggtc ttccagatca
     3421 tcctcgtgct ctgccttccc gtcgcggcct ttctcgtgat gcgcgacaag acgctctcgc
     3481 ccgacggcga ggagatggag cagaagaagc gctggctcat gctcggcctc gtctcctttg
     3541 cctgcggcgc ctatgacggc ttctacggtc ccggcgcggg caccttcatg ctgctcggct
     3601 ttgcggccgc cgccaagctc ggcgtgcgcg acgcagccgg ccagatgaag atcgtcaact
     3661 tctcaagcgg catcgccgcc ctcgtgctct acgcggccac gggtcatgtc gactgggtgc
     3721 tcggcgccct tgccggatgc ttcggcatca cgggccacta catcggctcg ggcctcgtga
     3781 tgaagaacgg cggcaggatc gtgcgcccca tcattctcac ggtgctcgcc atcctcttcg
     3841 tgaagaccgc ctgggactat ctggcgtaga cgaagcctgg agaattcaga cggcaaaatg
     3901 catgagggcc gcgcagtgcg cggccctttt tcagcttgag gcggcttgga gctctctttt
     3961 ccatagaacc cgtgcgccgc aagcctctca tttcaatggg aggtaaggcg cacaaagttt
     4021 tcgaacgtga agtaacattg ccttatgcgc acctccacct cttcctacat cgtcgaactt
     4081 c
//
LOCUS       DBMDTI010000175         4079 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5128_length_4079_cov_2.600553, whole genome shotgun sequence.
ACCESSION   DBMDTI010000175 DBMDTI010000000
VERSION     DBMDTI010000175.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5128_length_4079_cov_2.600553"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="ACZPB6_07875"
     CDS             complement(<1..534)
                     /locus_tag="ACZPB6_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HIR1184419.1"
                     /translation="MNKFVPTALAAALCSAVFAASAAVPVITDGVSKSAALIHADPAM
                     QALLAEATSEEAQKWRFNTLLELARIASPSRFEMRRQAEITRRLVEEWGFAPEDVLTR
                     PDGFLKGAGVQTVDGKPVYNVCVRIPGTYGARPDAVSYKGQLPKVVLEGHIDTVNPAV
                     LPPAETPYEAVKLQKVSE"
     gene            complement(754..1179)
                     /locus_tag="ACZPB6_07880"
     CDS             complement(754..1179)
                     /locus_tag="ACZPB6_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019307.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HIR1184420.1"
                     /translation="MKNIRTILRPAAALLLSLAAAGAMAGSQSVAVSIVGPGGHSNGD
                     YGNVNAVHAAARSIMLIEKSVPDAVVTAVTGGNSVNSIAAYANFRVLLEGDDAALKAK
                     ADKVKAAVEEGCKAENAFRGVKTGEVRDGLAADIRWTIK"
     gene            complement(1193..2887)
                     /locus_tag="ACZPB6_07885"
     CDS             complement(1193..2887)
                     /locus_tag="ACZPB6_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding metallopeptidase family protein"
                     /protein_id="HIR1184421.1"
                     /translation="MKFVPSLLAASICSAVFAASAAVPVVTDTIAKNAELMNADPAMQ
                     ALLKEATSEEAQKWRFNTLLELARIASPSRFEMRRQAEITRRLVEEWGFAPEDIMTRA
                     DGFLKGAGVQTVDGKPVYNVCVRIPGTYGARPDAVSYKGQLPKVVLEGHIDTVNPGVL
                     PPAETPYEAVKLQKVSDPIVKTREELKALALEVRFDKNGKVIEDEVWKKAYHRFNDLE
                     EAKKKGGYRIYVPGFEDAMINTVAVMQTAKLMKKHNIRPVYDIWVCGTTGEEGKGNLC
                     GMKQLYGYSQEAGKGNNALNIVANFGADSTMPGDAIVNYIGSYRFEIKYTEPAGWKAG
                     EARPSAAMAMSRAIASISDLKTAWDLDKKAEKTTYTVGVASCDDPAKSRSTNCTLMVD
                     MRSPTQAPLSAIRARIEPEFKKAMDAENAKYGLKAGDKNAVSMELVWFGDRPAYQRPH
                     YNDIAMQAYWQASKTADIDVVKAVPEEAASLNDNVPAAVGVPTVNVNLGTVAGGGGGH
                     TWYEWGVPGNGVDEGKRLYRFLMMGLLASGYNMTDGKVLEPGVGPMGNRTTEEMFK"
     gene            complement(3139..3990)
                     /gene="pssA"
                     /locus_tag="ACZPB6_07890"
     CDS             complement(3139..3990)
                     /gene="pssA"
                     /locus_tag="ACZPB6_07890"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR1184422.1"
                     /translation="MSNNNAYHRESLASISQRRLSTVKSKSIYVLPNAFTAAALFSAF
                     YGIIAAMNGDFYAAAIAVFFSMLFDGMDGRVARLTHTQSEFGVQMDSLADAVSFGVAP
                     GLIVYEYTLKGLGKLGWAIAFIYCLCAILRLARFNCNVGVVSKNYFQGLPSPAAAALV
                     CGFVWLGVEGKLPEWLLPYQSEIAAVVTLYAGLTMVSNAPFFSGKSYAVVRTIPFWFV
                     VVFALLFIVISSNTSLSVFVLFCIYALSGFAYGFYCWWTGRPNPIQADIAAALAAEAE
                     EHEDTQH"
BASE COUNT          671 a         1116 c         1497 g          795 t
ORIGIN      
        1 ttcgctcacc ttctggagct tcacggcttc gtacggggtt tcggcgggcg gaaggacggc
       61 cggattcacg gtgtcgatgt ggccttcaag gacgaccttc gggagctggc ccttgtagga
      121 gacggcgtcg ggacgggcgc cgtaggtgcc gggaatgcgc acgcagacgt tgtagacggg
      181 cttgccgtcg acggtctgaa cgcctgcgcc cttcagaaag ccgtcgggac gcgtcaggac
      241 gtcctcgggg gcaaaacccc actcctcaac gaggcggcgg gtgatttcgg cctggcggcg
      301 catttcaaag cgcgacgggg aggcgatgcg ggcgagttcg agcagcgtgt tgaagcgcca
      361 cttctgggcc tcttcactcg tggcttcggc cagaagcgcc tgcatggcgg gatcggcgtg
      421 aatgagggct gcgctcttgg agacgccgtc ggtgatgacg ggaacggctg cggaggctgc
      481 gaagacggcc gagcagagag cggccgcgag agctgtggga acgaacttgt tcaaggtctt
      541 tctcctcttg aaggccggag ggccgaaggg aggcggcgcc ggtccgggcg ccgcaggtca
      601 tccggcgaac aagtcgagtg taggtagcct gaaggcgacg tttcaattgc ggttaacgcc
      661 tatggacggg tttcggatga cggaaaacaa aacgcccctg ccggggcctt gtgaagggct
      721 cgggcagggg ctggggactc gcaagttgag gagttacttg atcgtccagc ggatgtcggc
      781 ggcgagaccg tcgcggactt cgccggtctt gacgccgcgg aaggcgtttt cagccttgca
      841 gccttcctcg acggcggcct ttaccttgtc ggccttggcc ttgagagcgg cgtcgtcgcc
      901 ttcaagaagg acgcggaagt tggcgtaggc tgcgatcgag ttgacggagt tgccgcccgt
      961 gactgccgtg acgacggcgt cgggaacgga cttctcgatc agcatgatgc tgcgggcagc
     1021 ggcatgcacg gcgttgacgt tgccatagtc gccgttggag tggccgcccg ggccgacgat
     1081 cgagactgcg actgactggg agccggccat ggcgccggcg gccgcgaggc tcagaagaag
     1141 agcggcggcg ggacgaagga ttgtgcggat gttcttcatg atgacgctcc tcttacttga
     1201 acatttcttc ggtggtgcgg ttgcccatcg ggccgacgcc cggttcgagc accttgccat
     1261 cggtcatgtt gtagcctgag gcgagcaggc ccatcatgag gaagcggtag agacgcttgc
     1321 cttcatcgac gccgttgccc gggacgcccc attcgtacca ggtgtgaccg ccgccgccac
     1381 cggcgacggt gccgaggttg acgttgacgg tgggcacgcc gactgcggcg ggcacgttgt
     1441 cgttgagcga ggcggcttct tcaggaacgg ccttcacgac gtcgatgtcc gcggtcttgg
     1501 aggcctgcca gtaggcctgc atggcgatgt cgttgtagtg cgggcgctga taggcgggac
     1561 ggtcgccgaa ccagacgagt tccatcgaga cggcgttctt gtcgccggcc ttgagaccgt
     1621 acttggcgtt ttcggcatcc atggccttct tgaattcagg ctcgatccgg gcgcggatgg
     1681 cggagagcgg agcctgcgtc gggctgcgca tgtcgaccat cagcgtgcag ttggtcgagc
     1741 ggctcttggc gggatcgtcg caggaggcca cgccgacggt gtaggtcgtc ttttcagcct
     1801 tcttgtcgag gtcccaggcg gtcttgaggt cggagatcga ggcgatcgcg cggctcatgg
     1861 ccatggcggc ggacggacgt gcttcgccgg ccttccagcc ggcgggttcc gtgtacttga
     1921 tttcgaagcg gtaggagccg atgtagttga cgatggcgtc gcccggcatc gtgctgtcgg
     1981 cgccgaagtt ggcgacgatg ttgagggcgt tgttgccctt gccggcttcc tggctgtagc
     2041 cgtagagctg cttcatgccg cagaggttgc ccttgccttc ctcacccgtc gtgccgcaga
     2101 cccagatgtc gtagaccgga cggatgttgt gcttcttcat gagcttggcg gtctgcatga
     2161 cggcgaccgt gttgatcatg gcgtcctcga agcccggcac gtagatgcgg tagccgccct
     2221 tcttcttggc ttcctcgagg tcgttgaagc ggtggtaggc cttcttccat acctcgtcct
     2281 cgatcacctt gccgttcttg tcgaagcgga cttcaagggc gagcgccttg agttcctcgc
     2341 gcgtcttgac gatcgggtcg ctcactttct ggagcttgac ggcttcgtac ggggtttcgg
     2401 ccggcggcag gacgcccggg ttcacggtgt cgatgtggcc ttcaaggacg accttcggga
     2461 gctgcccctt gtaggagacg gcgtcgggac gggcgccgta ggtgccggga atgcgcacgc
     2521 agacgttgta gacgggcttg ccgtcaacgg tctgaacgcc ggcgcccttg aggaagccgt
     2581 cggcacgggt catgatgtct tcaggcgcaa agccccactc ctcgacgagg cggcgggtga
     2641 tttcggcctg gcggcgcatt tcaaagcgcg acggggaggc gatgcgggcg agttcgagca
     2701 gcgtgttgaa gcgccacttc tgggcctcct cgctcgtggc ctccttcagc agggcctgca
     2761 tggccgggtc ggcgttcatg agttcggcgt tcttcgcgat cgtgtcggtg acgaccggaa
     2821 cggcggcgga ggccgcaaag acggccgagc agatgctcgc ggccagaagg ctgggaacaa
     2881 atttcatctt gattctcctc atttcggatg cgggtggtcg gggggcgggg cgtctctgtg
     2941 gtctgcgccc tgcgtgttcg ccgcatctcg acggaagagt ctagaaaaag tttccgttgc
     3001 gtccaattgc cgtttacgac tatgggaagt gtcgcatata agcgaaaacc attgcgtaga
     3061 aagacggatg acgggcatga aaaacgccgt cgctttcggg gcgacggcgt ttggaggggt
     3121 tcgggtgagt gtccgaggtc agtgctgcgt gtcctcgtgc tcctcggcct cggccgcgag
     3181 tgcggcggcg atgtcggcct ggatcgggtt cggacggccg gtccaccagc agtagaagcc
     3241 gtaggcgaag cccgagaggg cgtagatgca gaagaggacg aagacggaga gcgaggtgtt
     3301 gctcgagatc acgatgaaga gaagcgcgaa caccacgacg aaccagaacg ggatcgtgcg
     3361 cacgacggcg tagctcttgc cggagaagaa gggcgcgttg gagaccatcg tgaggccggc
     3421 gtagagcgtg acgacggcgg cgatttcact ctggtacggc aggagccatt cggggagctt
     3481 gccctcgacg ccgagccaga cgaagccgca gacgagcgcg gcggcggccg gagagggaag
     3541 gccctggaag tagttcttgc tcacgacgcc cacgttgcag ttgaagcggg cgaggcggag
     3601 gatggcgcac aggcagtaga tgaaggcgat ggcccagccg agcttgccga ggcccttgag
     3661 ggtgtattcg tagacgatga ggccgggggc gacgccgaag ctgacggcgt cggcgaggga
     3721 gtccatctgg acgccgaatt cggactgggt gtgcgtgagt cgggcgacgc ggccgtccat
     3781 gccgtcgaaa agcatggaga agaagaccgc gatcgcggcg gcgtagaagt cgccgttcat
     3841 ggcggcgatg atgccgtaga aggctgagaa gagcgcggca gcggtgaatg cgttcggaag
     3901 aacgtagatg ctcttgcttt tgaccgtgct gaggcggcgc tggctgatcg aggccaggct
     3961 ttcgcgatgg taggcgttgt tgttgctcat ctcttttgtg attcgttcgg ttcgttgaat
     4021 gacgcgggga cgtggcactc cctgcgtctg cgcaacggcg cgaaggccgt ggcggcggt
//
LOCUS       DBMDTI010000176         4073 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5140_length_4073_cov_2.392246, whole genome shotgun sequence.
ACCESSION   DBMDTI010000176 DBMDTI010000000
VERSION     DBMDTI010000176.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5140_length_4073_cov_2.392246"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="ACZPB6_07895"
     CDS             <1..1130
                     /locus_tag="ACZPB6_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR1184423.1"
                     /translation="LPLEVHFDKNGKVIEDEIWQKAYKRYQNIEDARKKDGYRIYVPG
                     YNDAMINTVAVMQVAKLMKKHNIRPVYDIWVCGTTGEEGKGNLCGMKQLYGYNQDVGK
                     GNNALNIVANFGADSTKPGSAIVNYLGSYRFEIKYVEPEGWKPGDKRPSAAMAMSRAV
                     AAISGLKTPWDADKSAERTTYTVGVAKCEGPKARSTACTLMVDMRSPTQGPLSAIRAR
                     IEPEFQKAMDAENARYGLKAGDKNAVSMELVWFGDRPAYQRPHYNDIAMQALWQASKT
                     AGIDVIDKVPERAASLNDNVPAAVGVPTVNTNVGTNAGGGGGHTWYEWGIPGNGVDEG
                     KRVYRFLMMGLLASGYNLADGSVVEPNVGPMGNRTTEEMFK"
     gene            1143..1568
                     /locus_tag="ACZPB6_07900"
     CDS             1143..1568
                     /locus_tag="ACZPB6_07900"
                     /inference="COORDINATES: protein motif:HMM:NF019307.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HIR1184424.1"
                     /translation="MLNIRTLIRPAAALLLAASAACAMAAGQTLAVSIIGPGGHSNGN
                     YGHVNAVHAAARSIMLIEKAVPDAVVSDIQGGNSVNSIAASADFIVTITGDEASLKAQ
                     ADKVKAAVEEGCKAENAFRGVKTGEVRNGVASDIRWSVK"
     gene            1718..2761
                     /locus_tag="ACZPB6_07905"
     CDS             1718..2761
                     /locus_tag="ACZPB6_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184425.1"
                     /translation="MTNANETSASLFAWMPFYSELATKLLAFKNNRKTLLSRLQAVFD
                     DHPNLDFPAFDGIPDDIDPFTVFAFVNRGLSRENATLTCAGFKRAFGLASEVPELFPG
                     LPRMDNRNVYFFRSREHRSDDVDKLWQAFEAALTLANDDTEAHRRTFAEAFDAALALP
                     GIKWNLTTGLFWIRPSFYLSLDGINRWAIDACSLLGEAPSKMLHACKDTPPDGIANLS
                     LRDEAVKAIKASDTYTGIPDFSYRSWVKAQEVNEQNKRLKKCPDDEAQHSAKRTGWWL
                     RDIRANTGISFRLKTWSPSDGRTSAIRFNTSRARRFRRSSRRSILRSTNRPTIPGPSG
                     NLFTKSSRETSST"
     gene            2836..3855
                     /locus_tag="ACZPB6_07910"
     CDS             2836..3855
                     /locus_tag="ACZPB6_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019348.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1184426.1"
                     /translation="MPNLRRVSWTSVQERKTDWNLPVKTLTVYEAGSDKAASLEGLFE
                     NEDDFPEETARYAKEDFLNEVFMDEERVDRLLSLLEYKRNVILQGPPGVGKTFLARRL
                     ARLAMGEHDDSRIEFVQFHQSYAYEDFVMGLRPTAEGGFELRTGAFYDFCKKAEGDPD
                     RTYYFIIDEINRGNLSKIFSELFMLIEGDKRGQSVRLLYRDEQFSVPANLRLIGLMNT
                     ADRSLAMLDYALRRRFAFFELAPAFENERLKDELKEQGTPALEKLVAAIGRLNSEITD
                     DESLGRGFAVGHSCLFGHDGKLVNDDHLADIVEFELVPLIEEYWFDNPEQIRHWEVEL
                     RKALA"
     gene            3852..>4073
                     /locus_tag="ACZPB6_07915"
     CDS             3852..>4073
                     /locus_tag="ACZPB6_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184427.1"
                     /translation="MIPIKNVFYMLAYAFRALDRKAYRPLESEDFANAEDLLAAILIL
                     GVEDQFKRGVSRAYIEEESPLTAVRPDRGI"
BASE COUNT          900 a         1339 c         1121 g          713 t
ORIGIN      
        1 cgctcccgct tgaagtgcac ttcgacaaga acggcaaggt cattgaggac gaaatctggc
       61 agaaggccta caagcgctat cagaacatcg aggacgcccg aaagaaggac ggctaccgca
      121 tctacgtgcc cggctacaac gacgccatga tcaacacggt cgccgtcatg caggtcgcca
      181 agctcatgaa gaagcacaac atccgtcccg tctacgacat ctgggtctgc ggcaccacgg
      241 gcgaggaagg caagggcaac ctctgcggca tgaagcagct ctacggctac aaccaggacg
      301 tcggcaaggg caacaacgcc ctcaacatcg tcgccaactt cggcgccgac agcacgaagc
      361 cgggttccgc catcgtcaac tacctcggct cctaccgctt cgaaatcaag tacgtcgagc
      421 ccgaaggctg gaagccgggc gacaagcgtc cgtccgccgc catggccatg agccgtgcgg
      481 tcgccgccat ctcgggcctc aagacgccgt gggacgccga caagtccgcc gagcgcacga
      541 cctacacggt cggcgtcgcc aagtgcgagg ggccgaaggc gcgctccacg gcctgcacgc
      601 tgatggtcga catgcgcagt ccgacgcaag gtccgctctc cgccatccgc gcccggatcg
      661 agcctgaatt ccagaaggcc atggatgccg aaaacgccag gtacggcctc aaggccggcg
      721 acaagaatgc cgtctcgatg gaactcgtct ggttcggcga ccgtcccgcc taccagcgtc
      781 cgcactacaa tgacatcgcc atgcaggcgc tctggcaggc ctcgaagact gcaggcatcg
      841 acgtcatcga caaggttccc gaacgcgcgg cctcgctcaa tgacaacgtg cccgccgcag
      901 tcggcgtgcc caccgtcaac accaacgtcg gcacgaatgc cggcggtggc ggcggccaca
      961 cctggtacga atggggcatc ccgggcaacg gcgtcgatga aggcaagcgc gtctaccgct
     1021 tcctcatgat ggggctgctc gcctcgggct acaacctcgc cgacggctcg gtcgtcgaac
     1081 ccaacgtcgg tccgatgggc aaccgaacca ctgaagaaat gttcaagtaa ggaggcgccg
     1141 acatgctcaa catccgtacg ctcattcgtc ccgcagcggc gcttctgctc gccgcatcgg
     1201 ccgcctgcgc catggccgca ggccagacgc tcgccgtctc gatcatcggt cccggcggcc
     1261 actccaacgg caactacggc cacgtcaacg ccgtgcacgc cgccgcccgc agcatcatgc
     1321 tgatcgagaa ggccgtcccc gacgccgtcg tctccgacat ccagggcggc aactcggtca
     1381 actccatcgc cgcgtccgcc gacttcatcg tcacgatcac gggtgacgaa gcctcgctca
     1441 aggctcaggc cgacaaggtg aaggccgccg tcgaagaagg ctgcaaggcc gaaaacgcct
     1501 tccgcggcgt caagacaggc gaggtccgca acggcgtcgc ctccgacatc cgctggtccg
     1561 tgaagtaatc atccgtcacc tcccgcccct cgggcccgcc tcgaaagaga cgggcccgat
     1621 ttctttgtcc gcaagcgaca aaatccgtcg tacgacctcc tacaatgatt ccaatccaac
     1681 gccttacatt ttcgccaccc acacgtcaac acaagtcatg accaacgcca acgaaacctc
     1741 cgcctcgctc tttgcatgga tgccattcta ttcggaactc gccaccaagc tcctcgcctt
     1801 caagaacaac cgaaagaccc tgctcagccg tttgcaggcc gtcttcgacg accatccgaa
     1861 cctggacttt cccgcattcg acggcattcc cgacgacatt gatcccttca cggtctttgc
     1921 attcgtcaac cgcggtctat cacgagaaaa cgccacactg acctgcgccg gcttcaagag
     1981 ggccttcggc cttgcatccg aggttccgga actcttccct gggctgcctc gaatggacaa
     2041 ccgaaacgtc tacttcttcc gctcccgtga gcaccgaagc gacgacgtcg acaagctgtg
     2101 gcaggcattc gaagccgcgc tcacccttgc gaacgacgat acggaggcgc atcgcaggac
     2161 ctttgccgaa gcgtttgacg ccgctcttgc cctgcctggc atcaagtgga accttacgac
     2221 aggccttttc tggatcaggc cgagtttcta tctgagtctg gacggcatca atcgatgggc
     2281 gatagacgcg tgctcgctcc tgggagaagc accctcgaaa atgcttcacg cctgcaagga
     2341 cacgccgccc gacggcatcg ccaacctcag ccttcgtgat gaagccgtca aagccatcaa
     2401 ggcctccgac acatacacgg gcattcccga cttcagttat cgctcctggg tcaaggcgca
     2461 agaagtcaat gaacagaaca agcgtttgaa gaagtgcccc gacgacgaag cccaacattc
     2521 agcgaagcgt actggctggt ggctccggga tattagggcg aatactggga tcagtttcag
     2581 gctgaaaaca tggtcgccat cggatggaag gacatcggcg atccgcttca atacgagtcg
     2641 cgccaggaga ttcagaagaa gctcaaggag gtctatcctt cggagcacga accgtccaac
     2701 aattcccggg ccatctggca atttgttcac gaaatcaagc cgggagacgt cgtctacgtg
     2761 aaaggcggcc gcaatctcgt ttacgggcgt ggcatcgtca cctccgactt catctacagt
     2821 ccggaaaatg ccgaaatgcc caatctgcgc cgcgtctcct ggacatccgt acaggagcgc
     2881 aaaacagact ggaaccttcc ggtcaaaacg ctgacggtgt acgaagccgg ctccgacaag
     2941 gcagcctcgc ttgaaggcct gttcgaaaat gaggacgact ttccggagga aacggcccgt
     3001 tatgcgaagg aggactttct caacgaggtc ttcatggacg aagagcgcgt ggatcgtctg
     3061 ctttcgctcc tcgagtacaa gcggaacgtc atccttcagg ggcctcccgg cgtgggcaag
     3121 acctttcttg ccaggcgcct tgccaggctt gccatgggag agcatgacga ctcgcgcatc
     3181 gagttcgtcc agttccacca gagctatgcc tacgaagact tcgtgatggg acttcgtccg
     3241 actgccgagg gtgggtttga actgcgcacg ggcgccttct acgacttctg caaaaaggct
     3301 gagggcgatc ccgataggac ctactacttc atcatcgacg aaatcaatcg aggcaatctc
     3361 agcaagatct tcagcgagct cttcatgctc atcgagggcg acaagcgcgg acagtccgtg
     3421 agactgctct acagggacga gcaattctcc gttccggcaa acctgcgcct tatcggcctc
     3481 atgaacacgg cggaccgcag ccttgcaatg ctcgactacg cgcttcgcag gcgctttgcg
     3541 ttcttcgaac tcgctcccgc cttcgagaac gagcgtttaa aggacgaact caaggaacag
     3601 ggcacgcccg cgctcgaaaa gctggtcgcc gccatcggta ggctcaacag cgagatcacc
     3661 gacgacgaga gcctcggacg cggctttgcc gtcggacaca gctgtctctt cggccacgac
     3721 ggaaagcttg tcaatgatga ccaccttgcc gacatcgtcg agttcgagct cgtgccgctc
     3781 atcgaggaat actggttcga caatcccgag cagatcaggc actgggaagt cgaactcagg
     3841 aaggctcttg catgattccc atcaagaacg tcttctacat gctcgcctat gccttccggg
     3901 cgcttgaccg gaaggcatac cgcccgcttg aatccgagga cttcgcaaat gccgaagacc
     3961 ttctcgccgc gatcctgatc ctcggcgtcg aagatcaatt caagcgcggc gtgagcaggg
     4021 cctacatcga ggaggagtct cccctgaccg ccgtgcgtcc ggatcgagga atc
//
LOCUS       DBMDTI010000177         4046 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5182_length_4046_cov_2.249176, whole genome shotgun sequence.
ACCESSION   DBMDTI010000177 DBMDTI010000000
VERSION     DBMDTI010000177.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5182_length_4046_cov_2.249176"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(19..171)
                     /locus_tag="ACZPB6_07920"
     CDS             complement(19..171)
                     /locus_tag="ACZPB6_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184428.1"
                     /translation="MSRYGIIVNVDRCAGCFACAVACKEENQVAPGIFWEKIKRDENV
                     LQRHQT"
     gene            complement(184..2391)
                     /locus_tag="ACZPB6_07925"
     CDS             complement(184..2391)
                     /locus_tag="ACZPB6_07925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR1184429.1"
                     /translation="MTGPKIARRSLLASAAATAAAPATSLFAPLAHAADNDAWTGYTI
                     CDSCNHMPMCGIQFEAVGNTVTGISNWKEHPNNSLCSKGISTLQRLYNPNRLLYPIKR
                     TAPKGSKDPGWVRISWDEAIKTIAERLSAVKAKHGADRVMFYCGDPKEPRPPVMRLAR
                     YFGSPNYCCESSVACNLAMVHALELSYGGEISGGMGPKTKCLMIVGQNGAWSKPHGFF
                     RGLLAAKARGMKLIVMDVRRTKVAEQADIHLQPRGGTDGAVAWGMMRVLIKEGLVNKP
                     FIDKWCIGYDELVAYAEKFTPEYVEKESGVPAELVVKAARMFADGPSGITISGQSIMH
                     QHNGCNNARACSLLMALTGNVENAGGGMLPNWPEDYLRWDEGYTRSFIDQKWFNEPEQ
                     KAKRIDREFVPVWNEMQVLCSPNMLPEMVRDGRIRAIAGFGMNALIWPSPEQYQEAIR
                     KVDFSFAADNFYRDETHHDMDIVLPAALNFERYAPFGVHGRKVSVRRPVKPQGEAWED
                     WKIACTIGAAVCDPETFFHGDPVKACDSILKDWGVTYEKQQGVLPNLQTVEFFPPQTT
                     GKHEKALLRFDGKPGFRTPSGKIELVASLQAKHGFPGLPVYVEPPKPTAEYPFKLLNG
                     TRRPYITHSKTRSDQPYLLELEPESTINMHPKDAEKLGIKEGDGVWLVSPYCDHKVRA
                     KVRVTILCQPGLIDAQYGWRGDQETQPLIPRKNWDPISGYPCCNDVCIRVEKA"
     gene            complement(2784..3500)
                     /locus_tag="ACZPB6_07930"
     CDS             complement(2784..3500)
                     /locus_tag="ACZPB6_07930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1184430.1"
                     /translation="MPRHLLALALGVCAGVAVMVVAARGIALSRMLYLLAGLIFAGNL
                     FAALAFDYWSMLAARFIAGLPHGAYFGVSAIVATSLAAPGQASRDVCLMVTGMTIANL
                     VGVPLGSFLAWSLSWRATFAVVACAALLLILALRLWMPHVPALPNKGFRAQFRFLGHL
                     TPWLVLGAIGLGNGGFFACYSYVNPIMETVASVPTSLMSAVIALAGFGMVAGNLLSAR
                     LAGRFSDQALAWWIHLERKR"
     gene            complement(3743..>4046)
                     /locus_tag="ACZPB6_07935"
     CDS             complement(3743..>4046)
                     /locus_tag="ACZPB6_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1184431.1"
                     /translation="NRRVRSPFQPTGESFYAIMVTPAVHHTMGGIKIDSKTQVYNTKG
                     QVVPGLFAAGEITGGVHGGNRLGGNAQADIVTFGRIAGQQSYIYAMQAAAADKAKK"
BASE COUNT          701 a         1147 c         1418 g          780 t
ORIGIN      
        1 tacaggaaat cctgaccatt aagtctgatg acgttgtagg acgttttcgt cgcgcttgat
       61 cttttcccag aagatgccgg gagcgacctg gttttcttcc ttgcaggcga ccgcgcaggc
      121 aaagcacccg gcgcagcggt cgacgttgac gatgatgccg taacgggaca tggtgaacct
      181 cctttatgcc ttttcaacgc ggatgcagac gtcattgcag cacgggtagc ctgagatcgg
      241 gtcccagttc ttgcgcggaa tgagcggctg cgtttcctga tcgccgcgcc agccgtactg
      301 ggcgtcgatg aggccgggtt ggcagaggat cgtcacgcgg accttggcac gcaccttgtg
      361 gtcgcagtag ggcgagacga gccagacgcc gtcgccttcc ttgatgccga gcttttcggc
      421 gtccttcggg tgcatgttga tcgtgctctc gggttcgagc tcaagcaggt agggctggtc
      481 gctacgggtc ttcgagtgcg tgatgtacgg gcgcctcgtg ccgttgagga gcttgaacgg
      541 atattcggcc gtgggcttgg gcggctcgac gtagacgggc aggccgggga agccgtgctt
      601 ggcctgaagg ctcgcgacga gctcgatctt gcccgacggc gtcctgaagc cgggcttgcc
      661 gtcgaagcgc agaagcgcct tctcgtgctt gccggtcgtc tgcggcggga agaactctac
      721 ggtctgcagg ttcggaagca cgccctgctg cttttcatac gtgacgcccc agtccttgag
      781 gatcgagtcg caggccttga cgggatcgcc gtggaagaag gtctcggggt cgcagacggc
      841 ggcgccgatc gtgcaggcga tcttccagtc ctcccaggct tcgccctgag gcttcacggg
      901 acggcgcacg ctcaccttgc ggccgtgcac gccgaagggc gcgtagcgtt cgaagttgag
      961 ggcggcgggc aacacgatgt ccatgtcgtg atgcgtttcg tcacggtaga agttgtcggc
     1021 ggcaaacgag aagtcgacct tccggatggc ctcctgatac tgttcgggcg agggccagat
     1081 gagggcgttc atgccgaagc ccgcgatggc gcggatgcgg ccgtccctga ccatttcggg
     1141 gagcatgttc gggctgcaga gcacctgcat ttcgttccag acgggcacga actcgcggtc
     1201 gatgcgcttt gccttctgct cgggctcgtt gaaccacttc tggtcaatga aggatcgcgt
     1261 gtaaccttcg tcccatcgca aataatcttc gggccagttg ggcagcatgc cgccgcctgc
     1321 attctcgacg ttgcccgtaa gagccatgag cagcgagcag gcgcgggcgt tgttgcagcc
     1381 gttgtgttgg tgcatgatcg actggccgga gatggtgatg ccggacggcc cgtcggcgaa
     1441 catgcgcgcg gccttgacga cgagttcggc gggcacgccg gactccttct cgacgtattc
     1501 gggcgtgaac ttctcggcat aggcgacgag ttcgtcatag ccgatgcacc acttgtcgat
     1561 gaagggcttg ttgacgaggc cttccttgat gaggacgcgc atcatgcccc aggcgacggc
     1621 gccgtccgtg ccgccgcgcg gctgcaggtg aatgtcggcc tgttcggcga ccttggtgcg
     1681 gcgcacgtcc atgacgatga gcttcatgcc gcgggccttc gcggcgagaa gaccgcggaa
     1741 gaagccgtga ggcttcgacc atgcgccgtt ctggccgacg atcatcaggc acttggtctt
     1801 cgggcccatg ccgccggaga tttcaccgcc gtaggagagt tcgagcgcat gcaccatggc
     1861 gaggttgcag gccaccgagc tttcgcagca gtagttcgga gagccgaagt agcgcgcaag
     1921 gcgcatgacg ggcggacgcg gctccttcgg gtcgccgcag tagaacatca cgcggtcggc
     1981 gccgtgcttg gccttcacgg ccgaaagcct ttcggcgatg gtcttgatcg cttcgtccca
     2041 cgagatgcgg acccagcccg ggtccttgga gcccttgggt gcggtgcgct tgatcggata
     2101 gagtaggcgg ttcgggttgt agaggcgctg aagggtcgag atgccctttg agcacagcga
     2161 gttgttcgga tgctccttcc agttgctgat gcccgtgacg gtgttgccca ccgcttcaaa
     2221 ctgaatgccg cacatcggca tgtggttgca cgagtcgcag atggtgtagc ccgtccatgc
     2281 gtcattgtcg gccgcatgag caagcggcgc gaagagactg gtggcgggag cggctgcagt
     2341 ggcggctgcg cttgcaagga gcgagcgacg ggcgatcttg gggccggtca tgatggtttt
     2401 ctcctttgat cctcgggtcc tgatggaaag acggccctgg gggtgcgggg ctcgcatgaa
     2461 ccggggggct ttggccgcac ccgcgggacc gtctgcagcg cctgccgcgg ccgcgcatcg
     2521 gatcagtgcg tgcggcccgg agtcttcagg ctagtttcaa ttatcagaag cagtgcagat
     2581 ggcgtcaata ggatcaaacc ccgaagaaaa tagcccgaag ggtcggtttt cggagaatat
     2641 ttttgtcaac taaaggcgac ggcgactgca acgcctgttc tgcagtcgtt ttgaagagac
     2701 aggaggtctg tttcgctatt cctgtcgtga attgcgctca tttgagcaaa aacgagcttc
     2761 cttgattcag ctcagacgtt tcgtcagcgc ttgcgctcga ggtggatcca ccaggcgagc
     2821 gcttggtcgg aaaagcgccc ggcgagcctg gcggagagga ggttgcccgc gaccatgccg
     2881 aagcccgcga gcgcgatgac ggcgctcatg agcgaggtgg gcacgctcgc gaccgtctcc
     2941 atgatgggat tgacgtagct gtagcaggcg aagaagccgc cgttgccgag gccgatggcg
     3001 ccgagcacga gccagggcgt caggtgtccg agaaagcgga actgggccct gaagcccttg
     3061 ttgggcaggg cgggcacatg gggcatccag agcctgagcg ccagaatgag gaggagcgcc
     3121 gcacaggcca cgacggcgaa ggtggcgcgc cacgagagcg accacgcgag gaagctgccg
     3181 agcggcacgc cgacgaggtt ggcgatcgtc atgcccgtga ccatcaggca gacgtctcgc
     3241 gaggcctggc cgggcgccgc gagactggtc gccacgatgg cgctcacgcc gaagtaggcg
     3301 ccgtgcggaa ggcccgcgat gaatcgtgcg gcgagcatcg accagtagtc gaaggcgagg
     3361 gccgcgaaga ggtttcccgc gaagatcagg cctgccagga ggtagagcat gcgcgagagc
     3421 gcgatgccgc gcgcagcgac caccatgacg gcgacgcccg cacacacgcc gagcgcgagg
     3481 gcgagaaggt gtctgggcat gttgttgcct ttttggccgg cgtgcgtgcg ccgtgccttt
     3541 ggtgcgtttg ttgtattgac tgaggcttag ggaaaacccg ggtacggatt gtagcgcgaa
     3601 acgccgtgac acatcggggc ggcaggcgtt ttgagaaggg aaaaacgaaa gaactcccgc
     3661 ctgcagggca gacgggagtt cttggaggcc tgcaggacgc cgtccctgag gacggcgcct
     3721 tcagtcatcg gcttgagtcc gattacttct tggccttgtc ggcggcagcg gcctgcatcg
     3781 cgtagatgta ggactgctgg ccggcgatgc ggccgaaggt cacgatgtcg gcctgggcgt
     3841 taccgccgag gcggttgccg ccgtggacgc cgccggtgat ttcacccgca gcgaagaggc
     3901 ccggaacgac ctggcccttg gtgttgtaga cctgggtctt ggagtcgatc ttgatgccac
     3961 ccatggtgtg gtggacggcc ggcgtgacca tgatggcgta gaacgattcc cctgttggct
     4021 ggaaaggcga acgaacccgc ctgttc
//
LOCUS       DBMDTI010000178         4045 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5185_length_4045_cov_2.028398, whole genome shotgun sequence.
ACCESSION   DBMDTI010000178 DBMDTI010000000
VERSION     DBMDTI010000178.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5185_length_4045_cov_2.028398"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="ACZPB6_07940"
     CDS             <1..96
                     /locus_tag="ACZPB6_07940"
                     /inference="COORDINATES: protein motif:HMM:NF025890.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR1184432.1"
                     /translation="RDELECTNCHIMHGETENFCDQCHQFGYKVP"
     gene            380..2707
                     /locus_tag="ACZPB6_07945"
     CDS             380..2707
                     /locus_tag="ACZPB6_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR1184433.1"
                     /translation="MSVQDHLCSARIPTPVLTGLLIPASLLIILAIVPPHAFDLSVSK
                     LFFSDGWPWHRNEVFTTIFYRLPKLVPAIVATVLLVRLAVVLAQGRRILEEEASRRAL
                     YVVIAMGACAAAVWWLKSTTGVACPWSVEPFGGALPMTDPAFGLTDVPGRCWPSGHAG
                     TGFVFIAFYFALKDVRPRAAAGALLFAVAFGLLCGAVRVMEGAHFVSHVLVTGIVDWL
                     ICAALHALILEDRSSPAFAKPLSERGLVLLTAFWWTFVLNMPFLARAADLSEPNFAWS
                     MREALTLAGLFFGLLFISIALLTLVMALPRPVRRAVLVLLSLTGAIFFAGTLVYGIAF
                     DPNMARNVISTDVHEASAYFSARTLLLALAAGLPPVLAASAADMTPAGLRPAAVRGGV
                     AILALAAGAGALFTQMNTLAAVMRNDKALRYLITPVNVPYSLAATLARDASPDAAQKR
                     IVDPKPEFSVRLSRPAVLLVVIGETTRSASWGLAGYARDTTPALRAEGVISHPSVTAC
                     GSSTDVSLPCMLSRIGRSDYDRSRIIGEEALPSLLARAGAHVVWVDNQSGCKGTCAGT
                     EVRSPDPKSAACASGRCFDGVLLDELDADLANLPADRPTVLFYHMYGEHGPRYHADSP
                     AHAKVWTPECTDADLGACTKESIINAYDNAVRYTDGVLAGLVKRLKARTDIDAGFVYV
                     SDHGESLGEGGLYLHGAPYFMAPSEQIRVPMVMWLSKDWSRTFGFDAANLAREPAGGV
                     THEHLYSTVLGMLGVQSTSRRPRWDLTDNRSSAAQ"
     gene            2770..3462
                     /locus_tag="ACZPB6_07950"
     CDS             2770..3462
                     /locus_tag="ACZPB6_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1184434.1"
                     /translation="MSIRILVVDDNPDILANVRDFLSMKDGWAPETASTGREALERID
                     RSTFDLVILDVGLPDTDGMTLTRRLRASGFTAPILMLTARDTIDDRVEGLTSGADDYL
                     IKPFSLRELAARVEALLRRSGANPAGRLTAGPLSLDLKTLRVEREGRDIRLNPTCLQL
                     LRELMQQSPGIVSRRRLEAVLWQGEPPASDSLRSNLYLLRQAVDKPFDHPLIHTHPGL
                     GWSVADLPDSVS"
     gene            3472..>4045
                     /locus_tag="ACZPB6_07955"
     CDS             3472..>4045
                     /locus_tag="ACZPB6_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184435.1"
                     /translation="MPVRHSPPRSLAQRIMLSFALLVTFVAGFYAFALYQSIEFTEQQ
                     LISGILRDEVARAVDTLERGETPRLPEDTRLYGAPPLEAPPPAMRGLPEGFGELSNNG
                     DFFVHTGAWRGEPLVLTRDQLGFEDTERVFRRITLFSVLAVFFLGLLAGWILARNIMK
                     PVKTLSDEVRKASAARTYEPLSVELTNDEVG"
BASE COUNT          625 a         1542 c         1156 g          722 t
ORIGIN      
        1 cgcgacgaac ttgaatgcac gaactgccac atcatgcacg gcgagacgga aaacttctgc
       61 gaccagtgcc accagttcgg ctacaaggtt ccgtaatcac ccgaaccgtc atgcaggttc
      121 tcccacacct tcggtgagga tgctctcctc catatctcct gcctcaccga aagcctctcc
      181 cccgaaagcg ctctccggcc ggtcaccgga agagcgcttt tttcttcccc cgcccatctg
      241 catgcctcat gacatggcgc aaggtctttg acgctccttt gacggacgct tcacggcgcg
      301 ttgacgaggc ctcacgcggt tttgacgcct cacgcccgat actgcggaca tcaagcaaaa
      361 acacgccgaa ggcatccgca tgtccgtcca agaccatctc tgctccgcac gcattccgac
      421 gcccgtcctc acgggccttc tcattcccgc gtcgcttctc atcatcctcg ccatcgtgcc
      481 gccgcacgcc ttcgacctct ccgtctcgaa gctcttcttc agcgacggct ggccctggca
      541 caggaacgaa gtcttcacga cgatcttcta ccggcttccg aagctcgtgc ccgccatcgt
      601 tgcgacggtg cttctcgtcc gtctcgccgt cgtgctcgcc cagggccgca ggattctcga
      661 ggaggaggcc tcgagacgcg cactctacgt cgtcatcgca atgggcgcct gtgccgccgc
      721 agtctggtgg ctcaagagca cgacgggtgt cgcgtgcccc tggtcggtcg agcccttcgg
      781 cggcgccctt ccgatgacgg atcccgcctt cggcctcacg gacgttcccg gccgctgctg
      841 gccctcggga cacgcgggca cgggcttcgt cttcattgcc ttctactttg cgctcaagga
      901 cgtccgcccg cgtgctgccg caggcgcact tctcttcgcc gtcgccttcg ggctcctgtg
      961 cggcgcggtc cgcgtcatgg aaggcgcgca cttcgtctcg cacgtgctcg tcacgggcat
     1021 cgtcgactgg ctcatctgcg cggcgctcca cgcgctcatc ctcgaggacc gcagctcgcc
     1081 cgccttcgca aagccgctct ccgaaagggg cctcgtcctc ctgacagcct tctggtggac
     1141 gttcgtcctc aacatgccgt ttcttgcacg cgccgcagac ctctccgagc cgaacttcgc
     1201 ctggagcatg cgcgaggccc tgacgctcgc gggcctcttc ttcggccttc tcttcatctc
     1261 catagcgctc ctcacgctcg tcatggcgct gcctcgtccc gtgcgccgcg ccgttctcgt
     1321 gctcctgagc ctgaccggcg cgatcttctt cgcgggcacg ctcgtctacg gcattgcgtt
     1381 cgacccgaac atggcccgca acgtcatctc caccgacgta cacgaggcct cagcctattt
     1441 ctccgcacgc acgctcctgc tcgctctggc cgcaggcctt ccgcccgtcc tcgctgcctc
     1501 cgcggccgac atgacgcccg ccggcctcag gccggccgcc gtgcgtggcg gcgtcgccat
     1561 ccttgcactc gccgcaggtg caggcgcgct cttcacgcag atgaacacgc ttgcggccgt
     1621 catgcgaaac gacaaggcgc tgcgctacct catcacgccc gtcaacgtcc cctacagcct
     1681 cgccgccacg ctcgcaaggg acgcctcgcc cgatgcggcc caaaagcgca tcgtcgatcc
     1741 gaagcccgag ttctccgtca ggctcagccg tcccgccgtc cttctcgtcg tcatcggcga
     1801 gaccacccgc tcggcgagct gggggctcgc aggctacgcc cgcgacacca cgcccgcgct
     1861 tcgcgccgaa ggcgtgatca gccatccgtc cgtcacggcc tgcggctcga gcaccgacgt
     1921 ttcgcttccc tgcatgctga gcaggatcgg ccgctccgac tacgaccgca gccgcatcat
     1981 cggcgaggag gctctgccgt cgctcctcgc ccgtgcgggc gcgcacgtcg tctgggtcga
     2041 caaccagagc ggttgcaagg gcacgtgtgc gggcaccgag gtacgaagcc ccgacccgaa
     2101 gagcgccgcc tgcgcctcgg gccggtgctt tgacggcgtc cttctcgacg agctcgacgc
     2161 cgacctcgcg aaccttcccg ccgaccgtcc gacggtgctc ttctaccaca tgtacggcga
     2221 gcacggcccg cgctaccacg cggactcgcc cgcgcacgcc aaggtctgga cgcccgaatg
     2281 caccgacgca gacctcggcg cctgcacgaa ggagagcatc atcaacgcct acgacaacgc
     2341 cgtgcgctac accgacggcg tgctggcggg cctcgtgaaa aggctcaagg cccgaacgga
     2401 catcgacgcg ggcttcgtct acgtctccga ccacggcgag agcctcggcg aaggcggcct
     2461 ctacctgcac ggcgccccgt acttcatggc gccgtccgag cagatccgcg tgccgatggt
     2521 catgtggctc tcgaaggact ggagccgcac cttcgggttt gatgcggcga atcttgcccg
     2581 cgagcctgcg ggcggcgtca cgcacgagca cctctactcg accgtgctcg gcatgctcgg
     2641 cgtgcagtcg acgagccgac gccccagatg ggacctgacg gacaatcggt cgagcgccgc
     2701 acaatgacgc gcgcaggctg caaggcgtaa catccctgca ggaagactaa tcagaaaaga
     2761 agcactctca tgagcatccg cattctcgtc gtcgacgaca atcccgacat ccttgccaac
     2821 gtccgcgact tcctttccat gaaggacggc tgggcgcccg agacggcctc cacggggcgc
     2881 gaggccctcg agcgcatcga ccgctcgacc ttcgacctcg tcatcctcga cgtggggctg
     2941 cccgacaccg acggcatgac gctcacccga aggcttcgcg cctcgggctt caccgcgccg
     3001 atcctcatgc tcacggcgcg cgacacgatc gacgaccgcg tcgaggggct cacgagcggc
     3061 gcagacgact atctgatcaa gcccttctcg cttcgcgagc tcgccgcccg cgtcgaggcc
     3121 ctgctgcgcc gaagcggcgc gaatccggcg gggcgcctca ctgcgggccc gctctcgctt
     3181 gacctcaaaa cgcttcgcgt cgagcgcgaa ggccgtgaca tcaggctcaa cccgacctgc
     3241 ctgcagctcc ttcgcgagct catgcagcag agtcccggca tcgtctcgcg ccgtcgactc
     3301 gaggcggtcc tctggcaggg cgagccgccc gcctccgaca gcctgcgctc gaacctttat
     3361 cttctgcgcc aggcggtcga caagccgttc gaccatccgc tcatccacac gcatcctggc
     3421 ctcggctggt ccgttgccga tcttcccgac tccgtctcct gaaacgccgc catgcccgtc
     3481 cgacattcgc ctccgcgcag cctggcccag cgcatcatgc tgagcttcgc gctcctcgtc
     3541 accttcgtgg ctgggttcta cgcattcgcg ctctaccagt cgatcgaatt caccgagcag
     3601 cagctcatct cgggcatcct cagggacgag gtcgccaggg cggtcgacac gcttgagcgc
     3661 ggcgagacgc cgaggctccc cgaggacacc cggctctacg gcgcgcctcc ccttgaggcg
     3721 cctccgcccg ccatgcgcgg cctgcccgaa ggcttcggag aactttcgaa caacggcgac
     3781 ttcttcgtcc atacaggcgc ctggcgcggt gagccgctcg tgctcacgcg cgatcagctc
     3841 ggcttcgagg acacggaacg cgtcttcagg cggatcacgc tcttttccgt tctcgccgtc
     3901 ttcttcctcg gccttctcgc gggctggatc cttgcgcgca acatcatgaa gcccgtaaag
     3961 acgctctccg acgaggtgag aaaggccagc gctgcccgca cctacgagcc gctttcggtc
     4021 gagctcacca acgacgaggt gggcg
//
LOCUS       DBMDTI010000179         4023 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5211_length_4023_cov_1.623406, whole genome shotgun sequence.
ACCESSION   DBMDTI010000179 DBMDTI010000000
VERSION     DBMDTI010000179.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5211_length_4023_cov_1.623406"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2372
                     /locus_tag="ACZPB6_07960"
     CDS             <1..2372
                     /locus_tag="ACZPB6_07960"
                     /inference="COORDINATES: protein motif:HMM:NF016932.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HIR1184436.1"
                     /translation="LVHLLCAESYLLRNEVDNAAAHLKASESLAKVEGNSYGYDLRSL
                     VKARKHTLQIIRALYEHKEDKAEKLFNKLTFEEQMEDPFVLRHMAETWAFQGFFKEEE
                     DHELFEKNLSKALETFGLYFVAGGSAPRAYQVTAVILSRLGNKAEMIETLEDGLDSVV
                     DFEDSDGVLTLSVMCRAAVTGGDRLCDETPRDYALDLTKAERIEWMRSQYERGDEGKR
                     AILEVTSPIPLDNFGRVELDFIARAALDMQDPQKLEAVLDVSGNNKDGNLIRLSLVCG
                     LLQLKGNWKEARHTASLAARLLFDGNIEQHFAVDTGLQLFTYVVEGFKQDGDMSVIDT
                     FVNNIKPEWGMRRQLVSMAAAGEILESSFGSKEDRVKKARGLLEALPEEGRDADWFFL
                     MLRCLVLAEDYDEAKKVAAKTRKFLDEYARNQIEQAEAQGDYGTQSELNMLRRNLAII
                     EIILKDHDSIKDFFNKSTVAELDKSNLKVERMIPNPNGPAIMVCSHPVDKHIVRIVAV
                     ADLAMKHVINAAQAEVTGSVTNEDAEAVKLPVLTIAAPAEEVKKINASDWRVLLLDAI
                     ARQMNKEGKMPEPGYMVTCTSSPTFPNTPFAGMCLCEAWDAVSASLNSITIYELVPLY
                     ANECDFAALYGHEKLLERLQKTNFWPVSIDRQDCCATSEWRPLIPKSQIRPMIEGPMA
                     TAYAWVTPGVLQKGEDIGIFYRRKPDGAFSGWVFLGTSERMAENFTSSKLLQIDLNTL
                     ANYAPYIVGFLDAEEKSAFRHDGCGSVSKIEYATMKYAGIPDAGGRRLS"
     gene            complement(2653..3576)
                     /locus_tag="ACZPB6_07965"
     CDS             complement(2653..3576)
                     /locus_tag="ACZPB6_07965"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1184437.1"
                     /translation="MKRRFAEFETWQIFVSLMRLGSMSRVAKETGYETSFVSRRIAAL
                     EKSVGSKLFERTTRLCMPTHEAHALLDRILPYVVDLERRLEMASRAVKATSGDVVLKV
                     RCASGLLPFVVRAIKPFRDNHPEYKFEVSAVHTPPVGSVMDADLGIWASSGTSPHFTF
                     EDLGVVPSWLCATPSFLERHSVRTVEDLDKVPLLGCSHWIAPFLIAPAVDPLLPVRRD
                     LRMAVNFNSVQGLRDAVLEDTGVGASLPAYTVATDIVKGRMVRVLPELTGPSLKFRLV
                     RQKTDIPVPEVERFARWLVESWRRDMVFPSA"
     gene            complement(3667..>4023)
                     /locus_tag="ACZPB6_07970"
     CDS             complement(3667..>4023)
                     /locus_tag="ACZPB6_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184438.1"
                     /translation="RHLRSRVHIAGRLRLQRHARRRLIELFNEFTLFIIVRQVSKATQ
                     IGLTKGEIQSSQHQQPRAFCCLQTPDKKGVPDADPDTPKDFCEKMQSSQEEKPAGRRR
                     FAHDGRKASRLQDNST"
BASE COUNT          884 a         1160 c         1211 g          768 t
ORIGIN      
        1 gtctggtgca tcttctctgt gcggaatcgt acctgctcag gaacgaggtc gacaacgctg
       61 ccgcacacct caaggcatcc gagagcttgg cgaaggtcga aggcaactcc tacggatatg
      121 atcttcgcag cctggtgaag gctcgcaagc acacccttca gatcatccgt gccttgtatg
      181 aacacaagga agacaaggcc gaaaagctct tcaacaagct cactttcgaa gagcagatgg
      241 aagacccgtt cgttcttcgt cacatggccg agacatgggc cttccagggc ttcttcaagg
      301 aggaagagga tcatgaactc ttcgagaaga acctcagcaa ggccctcgag actttcggtc
      361 tctacttcgt ggcaggaggg tctgcgccca gggcctacca ggttacggcc gtcattttga
      421 gcaggctggg caacaaggcc gagatgatcg aaacgcttga ggacggtctc gactcggtgg
      481 tcgactttga ggacagcgac ggcgtgctga ctctctcggt catgtgccgt gccgcagtca
      541 ccggaggcga ccggctctgc gacgaaacgc cgagggacta cgcactcgac ttgacgaagg
      601 ccgagcgcat cgagtggatg cgcagccagt atgagcgcgg tgacgagggc aagcgcgcga
      661 ttctcgaagt gacgtcgccg attcctctcg acaacttcgg ccgcgtcgag cttgatttca
      721 tcgcgcgtgc cgcacttgac atgcaggacc cgcagaagct tgaggcagtt ctggatgttt
      781 ccggcaacaa caaggacggc aacctcatca ggctctcgct cgtctgcggt ctgctccagc
      841 tgaaaggtaa ctggaaggag gcgcgccata ccgcatcctt ggctgcgcgc ttgctctttg
      901 acgggaacat tgagcagcat tttgccgtcg acacggggct gcagctcttc acgtacgtcg
      961 tcgaaggttt caagcaggat ggtgacatgt cggtgattga cactttcgtc aataacatca
     1021 agcctgagtg gggcatgcgt cgccaactcg tctccatggc ggccgcaggt gagatcctcg
     1081 agagtagctt cggcagcaag gaagaccgcg tgaaaaaggc caggggcctt cttgaagccc
     1141 tgcccgaaga gggccgggat gccgactggt tcttcctgat gctcaggtgc ctggttcttg
     1201 ccgaagacta cgacgaggcg aaaaaggtcg cagccaagac gaggaagttc ctcgacgaat
     1261 acgccaggaa ccagatcgag caggccgagg cgcaggggga ttacggcacc cagtccgagc
     1321 tgaacatgct tcgtcggaac ctcgcgatta ttgaaatcat tctcaaggat cacgactcca
     1381 tcaaggattt cttcaacaag agcaccgtcg ctgagctcga caagtcgaac ctcaaggtcg
     1441 aacgcatgat accgaacccg aacggccccg ccatcatggt ctgctctcat cctgtggaca
     1501 agcacatcgt gcgcatcgtc gccgtggccg acctggcgat gaagcatgta attaatgccg
     1561 ctcaggccga agtcaccgga tcggtcacga acgaagacgc cgaagcggtc aagctgcccg
     1621 tgctcacgat tgccgccccc gcagaagagg tgaagaaaat caacgcaagc gactggagag
     1681 tgcttcttct tgacgccatc gcacgtcaga tgaacaaaga aggcaagatg cccgagcccg
     1741 gctacatggt cacgtgcacc agttcgccca cgttccccaa cacgccgttt gccggcatgt
     1801 gcctttgcga agcctgggac gccgtctcgg cctcgctcaa ctcgatcacc atttatgagc
     1861 tcgtgccgct ctacgccaac gagtgcgatt ttgccgcgct ctacggccat gagaagcttc
     1921 ttgagcgcct gcagaagacg aacttctggc ccgtgtcgat cgatcgacaa gactgctgcg
     1981 ccacgtccga atggcgcccg ctcatcccca agagccagat ccgcccgatg atcgagggcc
     2041 cgatggccac ggcctatgcc tgggtgacgc cgggcgttct tcagaagggc gaggacatcg
     2101 gcattttcta ccgcaggaag cccgacgggg cattctcggg ctgggtgttc ctcggcacaa
     2161 gcgaacgcat ggccgagaac ttcacgtcct cgaaactcct tcagatcgac ctcaacacgc
     2221 tcgccaacta cgccccctac atcgtgggct tccttgatgc ggaggagaaa agcgccttca
     2281 ggcacgacgg ttgcggctcg gtctcgaaga tcgagtatgc caccatgaag tacgcaggca
     2341 ttcctgatgc cggcggcagg cgcctgagct gatccctcaa acctgcagac aaacgacaac
     2401 ttttccaaaa ccggcattct tgaacagtcg taccgaccag tccgatgacg aagcgcgttc
     2461 ccgccctcaa ggaaatcaag cgctacgtcc aggaagccca gcagctcgag cccatgctcg
     2521 aatactgata acgattgacg gcgtccgtca ggggcgccga gcccaaaagg gatgaatgca
     2581 gcatcccggg caaagaacaa ggccctgtct ccatgcggga ggcggggcct tcatgattct
     2641 tgggcggccg aatcaggcgg acggaaagac catgtctcgt cgccaggatt cgacgagcca
     2701 gcgtgcgaat cgttcgactt caggaacggg gatgtcggtc ttctggcgca cgagtcggaa
     2761 cttgagggac gggccggtga gttcgggaag gacgcggacc atgcggccct tgacgatgtc
     2821 ggtcgcgacc gtgtaggcgg gaagggaggc gccgacgccc gtatcttcga gtaccgcgtc
     2881 gcgaagtccc tgtacgctgt tgaagttgac cgccattctg agatcccgtc gaacgggaag
     2941 cagaggatca acggcggggg cgatgaggaa gggcgcaatc cagtgagagc agcccagaag
     3001 cggaaccttg tccagatctt cgacggtcct cacggaatgg cgctcgagga aggagggcgt
     3061 ggcgcagagc cagctcggca cgacgccgag gtcctcgaat gtgaagtgag gggacgtacc
     3121 cgaactcgcc cagatgccga ggtcggcgtc catgacgctg ccgacgggcg gggtgtgcac
     3181 ggcggagact tcaaacttgt actcgggatg attgtcgcgg aagggcttga tcgctcgcac
     3241 gacgaaggga agaagccctg atgcgcagcg aaccttgagg acgacgtcgc cggacgtggc
     3301 cttgacggcg cgggacgcca tctcgagtct gcgctcaagg tccacgacgt agggcaaaat
     3361 gcggtcaaga agggcatgcg cttcgtgcgt cggcatgcag aggcgcgtgg ttcgctcgaa
     3421 gagcttcgaa cccaccgact tttcgagcgc cgcaatgcgt cggctcacga acgaggtctc
     3481 gtagcccgtt tccttcgcca cgcggctcat gctgccgagc ctcatcagtg aaacgaagat
     3541 ctgccaggtt tcgaattcag caaagcgcct tttcatgagt taagtccagg aaaggtctgt
     3601 tgttctgacc gaatcctatt attattgcat aaacgcaacg cactgttgca acagtagtcg
     3661 tgcaacttaa gtagaattat cctgaagcct gctagccttg cgcccgtcat gcgcaaacct
     3721 cctccttccg gcgggtttct cttcttgaga agactgcatc ttctcgcaga agtcttttgg
     3781 cgtgtctgga tctgcatcag gcacgccttt tttatccgga gtttggaggc aacaaaaagc
     3841 ccgcggctgc tgatgctgcg agctctggat ctcccccttc gtcaggccga tctgggtggc
     3901 tttggacacc tggcggacga tgatgaagag ggtgaattcg ttgaataatt cgatcagtcg
     3961 acggcgagca tgacgttgga ggcgtagtcg cccagcgata tgaactcggg atcggagatg
     4021 ccg
//
LOCUS       DBMDTI010000180         3954 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5311_length_3954_cov_2.198806, whole genome shotgun sequence.
ACCESSION   DBMDTI010000180 DBMDTI010000000
VERSION     DBMDTI010000180.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5311_length_3954_cov_2.198806"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="ACZPB6_07975"
     CDS             complement(<1..713)
                     /locus_tag="ACZPB6_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1184439.1"
                     /translation="MMKSFKLLASIAAISAVFGCASTAPSMTDGTYTGVGQGRNGELT
                     VEVKVDAGKLSAVRVVKHVETVGISDAAVKQMPARIVDAQSLKVDAVSGATLTSEGIR
                     TAVADAIRKAGGDPAAFATAVVTKKAAARLIKEKADVVVVGAGGAGISAAVRAETLGA
                     KVILIEKMPVIGGATALNAGTLIATGSRFQREAMHETKDSPELATKDIFRVGKNRNDP
                     VLVKQVTERVGGVVDWLVY"
     gene            complement(792..1043)
                     /locus_tag="ACZPB6_07980"
     CDS             complement(792..1043)
                     /locus_tag="ACZPB6_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184440.1"
                     /translation="MNCTPHCFSGRTRHFFDRCRQRRIEDFEVDLALLFGVSKEDRNE
                     LGKKSCATFGRLNISRSGASFARRRSPCAPLRRFSGFAS"
     gene            1247..1618
                     /locus_tag="ACZPB6_07985"
     CDS             1247..1618
                     /locus_tag="ACZPB6_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184441.1"
                     /translation="MSRSHHIAKITSGAAIEHVRSYLLSEAARLGLGEEVDAADCRKL
                     LALLQDMRRSLETIERRGGLVVVSSTQTLITTYRCEGYRPQRKPHRCSPHLSMSERGF
                     IKERGLMHADGTMAAPPVRLW"
     gene            1753..>3954
                     /locus_tag="ACZPB6_07990"
     CDS             1753..>3954
                     /locus_tag="ACZPB6_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HIR1184442.1"
                     /translation="MTMQSLSTTTTELKPVELQSYLDEGWRIFVDTCSFMSSGCEPFF
                     TRSAAAFRDAGVSIHCPKEVRRELEKNLSSPKEQTRQLAMRGLAAYDALDKLDVLREI
                     NCPGSQENVFADPVFAKIFDEQRAEHKLLLITQDRRLAAGLYSRNQDEFMHFHPVHVL
                     RLLSRGDLGFLNHPQTQELMFPPNMNDFSRQSVPMIRAHLVSSEAVPPAGRSVPRTVS
                     AGRTGDVREASRPEKESVSKVVSKPAVPAVEKAAPALHAAFPVCTSVIDLEDRPVGID
                     RAPVEGDVVCDEFGMTHTLGRRLGEGGEGIVYDAGNGMVAKIYHADKCTTRREAKVRL
                     LASRPIRLKGVCAPQRMLFSNGFFAGCLMPEAKGFGLEGPLDKCFAGWSRADLVQLAV
                     TILKTIGSLHRLNVLVGDVNLSNIRIVSPDEAYLVDCDSVQVANLPCPVGHEMYTAPE
                     IRGRDFSTFLRSKSSENHAVAALIFMLLMPDEDLDESGKYRLGARRISSDPFLLDYPG
                     FHRGEPLTLCRRARIWSHLPDDLKSAFGRTFDSRGSLHAEADRLGVAGWILRLNAYRE
                     RLLDGTLLADDPLANEIYPKTSRKILPPARPACVVCGRPHNETDGRDGVCSTCISIAE
                     RVLARTTVRAAGTPAPSAPKSEPKASGPETAASEDKPSDTAYERTCVDCGSLFRITKG
                     EAEHLRNLGYRLPARCKACRAERRARKKAIERRSDEDGSILDEILNGVRDLI"
BASE COUNT          761 a         1355 c         1146 g          692 t
ORIGIN      
        1 tcgtagacga gccagtccac gacgccgccc acgcgttcgg tcacctgctt gacaagaacc
       61 ggatcattgc ggttcttgcc gacgcggaag atgtccttcg tggcaagctc gggcgagtcc
      121 ttcgtctcgt gcatggcctc gcgctggaag cgggagcccg tggcgatcag cgtgcccgcg
      181 ttgagcgccg tagcgccacc aatcaccggc atcttttcaa taaggatcac cttggcgccg
      241 agcgtttcgg cacgaacggc ggcggagatg cccgcgccgc ccgcaccgac cacgaccacg
      301 tcggcctttt ccttgatgag tctggcggca gccttcttcg tcacgacagc cgtcgcgaac
      361 gcggcgggat cgccgcccgc cttcctgatg gcgtcggcca cggcggtgcg gatgccctcg
      421 ctcgtgagcg tggcaccgga gacggcgtca accttgaggc tctgggcgtc gacgatgcgc
      481 gcaggcatct gcttgacggc cgcgtccgaa atgccgaccg tctcgacatg cttcacaacg
      541 cgaacggcgg aaagctttcc tgcgtcaacc ttgacctcga cggtcagctc gccgttcctg
      601 ccctgcccga cgcccgtgta tgtgccgtcc gtcatggacg gggcagtgga cgcacagccg
      661 aagaccgcgg aaatggccgc gatcgatgcc agaagcttga aggacttcat catgttctcc
      721 tctttggtct tgtggggcgg tgccgcctga gcggcacaac atcccgagtc tagaacgaca
      781 ggaacatgat gtcacgaggc aaaacccgaa aacctgcgta aaggcgcgca gggacttcgt
      841 cttcgggcaa aggaagcgcc tgatcttgaa atgttgagtc gcccgaatgt cgcgcagctc
      901 tttttgccga gctcgttgcg gtcttccttc gacacgccga acaaaagcgc gagatccacc
      961 tcgaagtcct cgatgcgccg ctgacggcag cgatcgaaaa aatgcctggt tcttccggaa
     1021 aaacaatgcg gcgtgcagtt catggttggt ctcctcatga cgggttgttc atgccctcat
     1081 gttaaatgac ggggagatcg gctccgaaac cgcaaatttg cggcctgatg tcctcagatc
     1141 gaacgccccg cccgcacctg caaccgcaaa tttgcgcttt ctccgagcac ctgcctccga
     1201 tcttaaagtg agacttgcca gacaaccgct cacaaaggag gacatcatga gccgctcgca
     1261 ccacatcgcc aaaatcacct ccggcgccgc catcgaacac gtccgcagct accttctctc
     1321 agaagccgcc aggctcggtc tcggcgagga agttgacgcc gccgactgca ggaagcttct
     1381 cgcgcttctt caggacatgc gcaggtcgct cgaaacgatt gagcgccgcg gcggtctcgt
     1441 tgtcgtttcg tcgacccaga ccctcatcac gacctaccgc tgcgaaggct accgcccaca
     1501 gcgaaagccc cacagatgca gtccccatct ttccatgtca gagaggggct tcatcaagga
     1561 gcggggcctc atgcacgccg acggcaccat ggccgcgcct cccgtgcgcc tctggtaaga
     1621 cgaggccggg aaaaggcaaa tttgcgaaat tccggctccg tcccatgcgc cttcacaatg
     1681 aacatgcatc ccgccgcaaa ggctccgcct tctgcggcgg acaacaaatc cttccaaccc
     1741 gagaaaaaag ccatgaccat gcagagcctc agcacgacca ccaccgaact caagcccgtt
     1801 gaactccagt cctaccttga cgaaggctgg aggatcttcg tcgatacatg ctccttcatg
     1861 tcctccgggt gcgagccgtt cttcacccga agcgccgcag ccttcaggga cgcaggcgtg
     1921 agcatccact gtccgaagga ggtgcgaaga gagcttgaga agaatctttc ctccccgaag
     1981 gagcagacgc gccagcttgc catgcgcggt ctggccgcct acgacgctct ggacaagctt
     2041 gacgtcctgc gcgagatcaa ctgtccgggc tcgcaggaaa acgtttttgc cgaccccgtc
     2101 ttcgcgaaga tctttgacga gcagcgcgcc gagcacaagc ttcttctcat cacccaggac
     2161 agaaggctcg ccgccggcct ctacagccgc aatcaggacg agttcatgca ctttcatccg
     2221 gtgcacgtgc ttcgcctgct ctcccgcggc gacctcggct ttctcaacca cccgcagact
     2281 caggagctta tgtttccgcc gaatatgaac gatttttccc gtcagtccgt accaatgatc
     2341 cgcgcccatc tcgtctcgtc cgaggcggtt ccgcccgcag gaaggtcggt cccgcgcacc
     2401 gtctccgcag gccgcaccgg cgacgtgcgc gaagcgtccc gtcctgagaa agagtccgtt
     2461 tcgaaggtcg tttccaagcc ggccgttccc gccgtcgaaa aggccgcgcc cgccctgcac
     2521 gccgccttcc ccgtctgcac gagcgtgatc gaccttgagg accgtcccgt gggcatcgac
     2581 agggcgcccg tcgagggcga cgtcgtctgt gacgaattcg gcatgaccca cacgctcggc
     2641 cgtaggctcg gcgagggcgg cgaaggcatc gtctacgacg caggcaacgg catggtcgcc
     2701 aagatctacc atgccgacaa gtgcacgacc cgccgggagg cgaaggtccg ccttctcgcc
     2761 tcgcgtccga tccgcctcaa gggcgtgtgc gcgccgcagc gcatgctctt ttccaacggc
     2821 ttcttcgcgg gctgcctgat gcccgaagcg aagggcttcg gactcgaggg accgctcgac
     2881 aaatgctttg cgggctggag ccgcgccgac ctcgtgcagc tcgccgtcac gatcctcaag
     2941 acgatcggga gcctgcacag gctcaacgtg ctcgtgggcg acgtcaacct cagcaacatc
     3001 aggatcgtct cgcccgacga agcgtatctc gtcgactgcg acagcgttca ggtcgccaac
     3061 cttccctgtc ccgtcggaca tgagatgtac acggcgcccg aaatccgcgg acgcgacttc
     3121 agcacgttcc tgcgctcgaa gagcagcgag aaccatgccg tcgcggccct gatcttcatg
     3181 ctcctcatgc ctgacgagga cctcgacgag agcggaaagt acaggctcgg cgcacggagg
     3241 atctcctcgg atccgttcct tctcgactat cccggcttcc accggggcga gccgctcacg
     3301 ctttgccgca gggcccgcat ctggagccat ctgcccgacg acctgaagtc ggcgttcgga
     3361 agaaccttcg acagcagagg gagcctccat gccgaggccg accgccttgg cgttgcggga
     3421 tggatcctcc gcctcaacgc ctatcgcgag cgtcttctcg acggcacgct tctcgcggac
     3481 gacccgctcg ccaacgagat ctatccgaag acgagcagga agatcctgcc tcccgcacgc
     3541 cccgcctgcg tcgtctgcgg caggccgcac aacgagaccg acggcaggga cggcgtctgc
     3601 agcacctgca tttcgatcgc cgagcgcgtg ctcgccagga cgacggtccg cgccgcaggc
     3661 actcccgcac cttccgctcc gaagagtgag ccgaaggcgt ccgggcccga gaccgcggca
     3721 agcgaagaca agccttccga caccgcgtac gaacgcacgt gcgttgactg cgggtcgctc
     3781 tttcgcatta cgaagggcga ggctgagcat ctgcgaaacc tcggctacag gctgcccgcg
     3841 cgctgcaagg catgccgcgc cgagcgccgc gccagaaaga aggcgatcga acgcagatcc
     3901 gacgaggacg gcagcatcct cgacgaaatc ctcaacgggg ttcgcgacct catc
//
LOCUS       DBMDTI010000181         3927 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5346_length_3927_cov_2.454783, whole genome shotgun sequence.
ACCESSION   DBMDTI010000181 DBMDTI010000000
VERSION     DBMDTI010000181.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5346_length_3927_cov_2.454783"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="ACZPB6_07995"
     CDS             <1..1317
                     /locus_tag="ACZPB6_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184443.1"
                     /translation="AERLQADGANLGLSEEQAERLRSAAEGAARFSYERPATVDAIML
                     MAERLRNRFGVNRTAEAAQFDVREDAKRVVWMLDHVFFSATVLRSDIEAAQYFQGENN
                     RGASMQLVDLVKSLNLRFVPEADLEKYAALWTNLAEQRTRGTFQGSDAVERVLLPSML
                     IVRGLWTFEAEANDADDYRHFISGRGDFARKRISDSRAGTQGEATFPTLSSMPRTGVP
                     FFRTLVWSAVLLAAVRRTLDTEADDYQQYITGQELEPLLCNGLVYWADAFMTDADGSM
                     PNRFEAFMASALEDPSQVNPDLLAEADIVKECAARLARDPAYEGWREALLLVMETLRL
                     LWERLDWRRIVRALALERTGECLLLVPYACGRAEETVAELFRRVRYRPSSMTGPNGHN
                     GDNEESKKEKKNHAHQVSKASKMVVVFDLYEACGSRSESIAAVFNS"
     gene            1327..2382
                     /locus_tag="ACZPB6_08000"
     CDS             1327..2382
                     /locus_tag="ACZPB6_08000"
                     /inference="COORDINATES: protein motif:HMM:NF015210.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HIR1184444.1"
                     /translation="MMKENTFAVSLTLEDLKRRRLVIPCFQRPYRWTRDEAAVLFDDL
                     MAFQEVAKLDSSIRYSLGTIVTARLDPRGEELFVLDGQQRLTTVALLENVLESHLDKE
                     MADEKAPLPPKWLSYRNLPEGASFPGRWKKAGRKEVFDSNGVRDAFEERLSMSKDDSV
                     GLDPERVLELLRNRVFFNLAEVPLESLAQTNDQEVHIEAAMMFENVNVRGVPLTDADI
                     LKAQLVERMDPDDADFYDQTWSAILKALALNSVEALPDLSKIEVKAVSTTHGGPLSDI
                     LKRSRGQKVRVLAGTGSDEGKGGRSSSTHAALDATMLLVTARVLWKALVNQDVDDYLT
                     REKFQLRFKPLLDKDEA"
     gene            2416..3393
                     /locus_tag="ACZPB6_08005"
     CDS             2416..3393
                     /locus_tag="ACZPB6_08005"
                     /inference="COORDINATES: protein motif:HMM:NF019151.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GmrSD restriction endonuclease domain-containing
                     protein"
                     /protein_id="HIR1184445.1"
                     /translation="MLVTARFVLEWCPNSGSEGLSFDNETNAKLLGDVGVELLETLMA
                     ESGGATGRYWIVLGVLLYRNAIVKNGGRGIEDVTSRNELKQAAHALKPGANKGFAKDL
                     VDRLKAWGARLAFEKGIVPSVELASQMANEEPQPLPDNSSTEHWLYPGRRYLFWTDWL
                     IRCDRRQNFDVMKKVFADLSTKFFKDPNSQVKFFKDLGDRIEKSRITFSGQVEHWVPQ
                     ADDRASRLLAGEARHLDEEPRQIDIFGNLALIDASLNSKLTRCGSKMKAEIVKQAGGN
                     LSVKLLWLTCWSELCRGDLDMTKDLPVLNAAWNQFALEATRKYAAKAGQ"
     gene            3551..>3927
                     /locus_tag="ACZPB6_08010"
     CDS             3551..>3927
                     /locus_tag="ACZPB6_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015210.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GmrSD restriction endonuclease domain-containing
                     protein"
                     /protein_id="HIR1184446.1"
                     /translation="MTEPTDAEHGERSIAVTFGEVLAGLQGMAEIPGMPELAEMPPAG
                     FTFQIPPCQRPYCWKLSDIEHVLADVADMRSSESTTPWYFGPICFRRDQNVMQIYDGQ
                     QRLTSMLLVVSVLAERLQADGANL"
BASE COUNT          808 a         1097 c         1224 g          798 t
ORIGIN      
        1 gccgaacgcc ttcaggcgga cggtgcaaac ctcggacttt cagaagaaca ggccgaaagg
       61 ctgcgaagcg ccgccgaagg cgccgcacgg ttcagctacg agcgtcccgc tacggttgat
      121 gccatcatgc tcatggcaga gcgtttgcgc aacaggttcg gcgtcaacag gacagcggag
      181 gcagctcagt ttgacgtgag agaggatgcg aaacgcgtcg tctggatgct cgatcacgtc
      241 ttcttcagcg ccaccgtttt gcgcagcgac atcgaggccg cacagtattt tcagggcgaa
      301 aacaaccgtg gtgcatcgat gcagctcgtc gacctcgtca agtcgctgaa cctcaggttt
      361 gtgcctgagg ctgatctcga aaagtatgcg gctctctgga cgaatctggc cgaacaaaga
      421 acgcggggta cttttcaggg aagcgatgcc gtcgagcgcg tgttgcttcc gtccatgctc
      481 attgtccgcg ggctctggac gttcgaggcc gaggcgaatg atgccgacga ctacaggcac
      541 ttcatcagcg gccgcggcga ttttgcaagg aaaaggattt cggactcaag ggccggtact
      601 cagggcgagg ccacgtttcc gacgctttcc tcgatgccgc gcacgggtgt tccgttcttc
      661 aggacacttg tctggagcgc ggttctgctt gctgccgtga ggcggacgct cgacactgag
      721 gcagatgatt atcagcagta catcacgggc caggaactcg agcctctgct ctgcaacgga
      781 cttgtttact gggccgacgc cttcatgacc gacgccgacg gcagcatgcc gaatcgtttc
      841 gaagccttca tggcgtcggc actcgaggat ccttcgcagg tgaatcccga cctcctcgcc
      901 gaggcggaca tcgtcaagga gtgtgccgct cgtcttgcgc gcgatcccgc ctatgaagga
      961 tggcgcgaag cgctccttct cgtgatggag acgttgagac tgttgtggga gaggctcgac
     1021 tggcgccgca tcgtcagggc gcttgcgctt gaaaggaccg gcgaatgcct gctgctcgtg
     1081 ccgtatgcct gcggccgggc ggaggagacg gttgcggagc tcttcaggcg agtccgatat
     1141 cgcccgtcgt cgatgacagg tccgaacggg cacaacgggg acaacgagga gagcaagaag
     1201 gaaaagaaga atcatgcgca ccaagtgagc aaggcaagca agatggttgt agtatttgat
     1261 ctgtatgagg cctgcgggag tcgttccgaa agcatcgccg ccgtgttcaa ttcgtaggag
     1321 tttttgatga tgaaggaaaa cacttttgcc gttagcctca ccttggagga cctcaagcgc
     1381 cgtcgtcttg tgattccgtg cttccagcgt ccataccgct ggaccagaga tgaggccgca
     1441 gtcctcttcg acgatctcat ggcttttcaa gaggtcgcga agctcgattc gtcgatccgc
     1501 tattcgctcg gcacgatcgt gacggcaagg ctcgatccca ggggcgaaga actcttcgtt
     1561 cttgatgggc agcagcgact gacgacggtc gcgcttctcg agaacgtact tgagagccat
     1621 cttgacaaag agatggctga cgagaaggcg ccgcttccgc cgaagtggct ttcctacagg
     1681 aatctgcctg agggagcgtc attccctggc aggtggaaga aggcgggacg gaaagaggtt
     1741 tttgattcca acggcgtaag agacgccttt gaggaaaggc tgtcgatgtc caaggacgat
     1801 tcggtggggc tggatcccga gcgcgttctc gaactgcttc gcaaccgcgt cttcttcaat
     1861 ctcgctgagg tgccgctgga gtctcttgcg cagacgaatg atcaggaggt gcacattgag
     1921 gctgccatga tgttcgagaa cgtcaacgtc cgtggcgtgc cgctcaccga cgccgacatt
     1981 ctcaaggcgc agcttgtcga gagaatggat cccgacgatg cggacttcta cgatcagaca
     2041 tggagcgcga tcctgaaggc gctcgctctg aactccgtcg aggccctgcc ggatctttcg
     2101 aaaatcgagg tcaaggcggt gagcaccacc catggcggcc cgctgtcaga catcctgaag
     2161 cgatcccgcg ggcagaaggt ccgcgtgctt gcgggcactg gttcggatga agggaagggg
     2221 ggacgtagtt cttcgacaca tgcggcgctc gatgcgacga tgctgcttgt caccgcccgc
     2281 gtgctgtgga aggctttggt gaatcaggac gtcgacgact acctgacgcg cgagaagttc
     2341 cagctccgct tcaagcctct tcttgacaag gatgaagcct gacgttgtgg gaacgcctgg
     2401 cagttcctgc acctgatgct tgtcaccgct cgcttcgtgc tcgaatggtg tccgaactcg
     2461 ggcagcgagg gcttgtcctt cgacaatgag acaaacgcaa agcttctcgg cgatgtcggc
     2521 gtggagcttc tcgagacact tatggccgaa tccggcggcg ccacgggccg atactggatc
     2581 gtcctcggcg tgctgcttta tcgcaacgcc atcgtgaaga acggcgggcg cgggattgag
     2641 gacgtgacgt caagaaacga gctgaagcag gctgcgcatg ctctcaagcc gggcgcaaac
     2701 aagggcttcg caaaggatct cgtcgatcgt ctcaaggcgt ggggtgcccg cctcgcattc
     2761 gaaaagggga tcgtcccctc cgtggaactg gcctctcaaa tggcgaacga ggagccgcag
     2821 cctcttccgg acaactcttc gacggagcac tggctctatc ccggccgccg ttatctattc
     2881 tggacggact ggctcatccg gtgcgaccgc agacagaatt tcgatgtgat gaagaaggtc
     2941 ttcgccgatt tgtcgaccaa gttcttcaaa gatcccaatt cacaggtcaa gttcttcaaa
     3001 gatctcgggg acaggattga aaagagccgc atcaccttca gtggtcaggt tgaacactgg
     3061 gtcccgcagg ctgacgacag ggcatcccgc cttctcgccg gggaagcccg tcatctcgat
     3121 gaagaacctc gccagattga tatttttggg aatctcgccc tcatcgacgc ctcgctcaac
     3181 agcaaactca cccgttgcgg cagtaagatg aaggccgaaa tcgtcaagca agccggcggc
     3241 aacctgagcg tgaaacttct gtggctcacc tgctggtcgg agctgtgccg aggggatctg
     3301 gacatgacga aggacctgcc cgtcctcaac gcggcctgga accagttcgc actggaggca
     3361 acacggaaat acgccgccaa agcaggccag tgattaagct gtgaacggta ccgcatgcgg
     3421 tgaggaaggt cgaggagatg cactgagcgg cgggcgcttt tcttcctgcc gtgtcggtgc
     3481 tccggcgtcg atataatttt tttcatgggc ctgcatttcg cgggccgagc cttgagaagg
     3541 gaacggagcc ttgactgagc ctactgatgc cgaacatggg gaaaggtcca tcgccgtgac
     3601 gttcggcgag gtgcttgccg gacttcaggg catggcggaa attccgggaa tgcctgaatt
     3661 ggcggaaatg ccgcctgcgg gcttcacgtt tcagatcccg ccgtgccaga ggccgtactg
     3721 ctggaagctc tccgacattg agcacgtgct cgccgacgtg gccgacatga ggtcatcaga
     3781 atcgacgacg ccctggtact tcgggccgat ctgcttcagg cgtgaccaga atgtcatgca
     3841 gatctacgac ggccagcagc gtctcacatc gatgcttctc gtggtctcgg tgctggccga
     3901 acgccttcag gcggacggtg caaacct
//
LOCUS       DBMDTI010000182         3903 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5384_length_3903_cov_2.774066, whole genome shotgun sequence.
ACCESSION   DBMDTI010000182 DBMDTI010000000
VERSION     DBMDTI010000182.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5384_length_3903_cov_2.774066"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="ACZPB6_08015"
     CDS             complement(<1..279)
                     /locus_tag="ACZPB6_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1184447.1"
                     /translation="MELKARSIKLFCAVVETGSLLSAANQNAMSAPAASRAVSQLEDR
                     LGCRLFDRSTKNLTLTEDGKAFYRVAKESMRAWHMLEDFPKQRASTKKQ"
     gene            529..1959
                     /gene="hydG"
                     /locus_tag="ACZPB6_08020"
     CDS             529..1959
                     /gene="hydG"
                     /locus_tag="ACZPB6_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HIR1184448.1"
                     /translation="MAIYDPKSRVAEEFINDQEIRDTLAYAEEHKSDVPLLMSILDKA
                     AQFKGLTHREAAVLLACDVKEVNDRIFETAIKVKEHIYGSRIVLFAPLYLSNYCVNTC
                     TYCPYHAKNKSIVRKKLTQEEIRNEVIALQDLGHKRLLLEAGEHPKHNPIEYILESIK
                     TIYSIKHKNGAIRRLNVNIAATTVENYRKLHEAEIGTYQLFQETYNRKAYEELHPKGP
                     KSDYAYHTEAHDRAMEGGIDDVGLGVLFGLDLCRYDFVGLLMHAEHLEAVWGVGPHTI
                     SVPRICPADDIDPASFENAVPDDLFLKIVALIRMAVPYTGMIMSTREGQRIRTEALKL
                     GISQISGGSRTSVGGYAIRSVQEMEEKTEQFETNDRRSLDEIVRWLLENHYLPSFCTA
                     CYRAGRTGDRFMEWAKDGSIATYCQANAIMTMKEYMCDYASKETREAGERVIANELPK
                     IPHEKIRIASEKRLIRIADGERDFRF"
     gene            complement(2143..2760)
                     /gene="ttdB"
                     /locus_tag="ACZPB6_08025"
     CDS             complement(2143..2760)
                     /gene="ttdB"
                     /locus_tag="ACZPB6_08025"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HIR1184449.1"
                     /translation="MTKKVLTTPVSDADIESLKIGDVFYLSGALITSRDMVHFRHVEE
                     GMDLPYDLAGKAIFHAGPIMVPDDKSRSGFRVVSIGPTTSMRMEKYEREFLRKTGVKI
                     VVGKGGMGPETAAGCQESKAVHCVFPGGCAVLAAECVEEVEERHWPELGQPESMWVMR
                     VKEFGPLIVSIDAHGGNLFEKNKAEFNRLKEPIVEEICSKVNYRH"
     gene            complement(2778..3677)
                     /gene="ttdA"
                     /locus_tag="ACZPB6_08030"
     CDS             complement(2778..3677)
                     /gene="ttdA"
                     /locus_tag="ACZPB6_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002163154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HIR1184450.1"
                     /translation="MNQQEKAERFTELMTRFTSVLGKILPDDVATRLAELRKEQTSDI
                     SRIVYDAMFDDLAAARRLNVPLCQDTGVIQYYIEVGANFPLLSEMRACLDEAVRRATI
                     EAPLRQNAVQIFDERNTGNNTGVRVPWIDWEIIPDSDSCLIHGYMAGGGCSLPGAAKV
                     LMPAEGYMGIVKFIFDVICDRGVNACPPLLVGIGIAGSVEVAAELSKKALMRPVGSKN
                     ANEIGADFEKMIEEGLNKINIGPGGLTGTKSVMGVNVEQAARHPSCLAVGVSTGCWGH
                     RRATIRINADMSYEMISHKGMTL"
BASE COUNT          785 a         1274 c         1121 g          723 t
ORIGIN      
        1 ctgcttcttc gtcgaggccc tctgcttcgg aaagtcctcg agcatgtgcc aggcgcgcat
       61 cgactccttc gccacgcgat agaacgcctt gccgtcctcc gtgagcgtca ggttcttcgt
      121 cgagcggtcg aagagcctgc agccgagcct gtcctcgagc tggctcaccg ccctgctcgc
      181 cgcgggcgcg ctcatcgcgt tctggttcgc ggccgagagg agcgaacccg tctccaccac
      241 cgcgcaaaag agcttgatcg accttgcctt cagttccatt tcatcctcct tccggacctc
      301 accgataatt tcattttccg caatctgccg tgcaatattt gcattttaca aaataaaaag
      361 cataaggcaa aatactttcc aacacggcag gcacggcctg agacacccag cctgcaactc
      421 tccgaaacac aagcaacacg gccgattcag aggacatctg atcggccgtt ttgcttttga
      481 ggcggagtgc acatcccaca agaccccagt caactcgacg agaaaaaaat ggccatttac
      541 gaccccaagt cccgcgtagc ggaagagttc atcaacgatc aggaaatccg cgacacgctc
      601 gcctacgccg aagagcacaa gtcggacgtt ccgctgctca tgagcatcct tgacaaggcc
      661 gcccagttca agggcctgac ccaccgcgaa gccgccgtgc tgctcgcctg cgacgtcaag
      721 gaggtcaacg acaggatctt cgagaccgcc atcaaggtga aggagcacat ctacggcagc
      781 cgcatcgtgc tcttcgcgcc gctctacctc tcgaactact gcgtcaacac ctgcacgtac
      841 tgcccgtacc atgccaagaa caagagcatc gtgcgcaaga agctcacgca ggaggagatc
      901 cgcaacgagg tgatcgcgct ccaggacctc ggccacaagc gcctgctcct tgaggcgggc
      961 gagcacccca agcacaatcc gatcgagtac atcctcgagt ccatcaagac catctactcg
     1021 atcaagcaca agaacggcgc catccgccgc ctcaacgtga acatcgccgc cacgacggtc
     1081 gagaactacc gcaagcttca cgaggccgag atcggcacct accagctctt ccaggagacc
     1141 tacaaccgca aggcttacga ggagctgcat ccgaagggac cgaagtccga ctacgcctac
     1201 cacaccgagg cgcacgaccg cgccatggaa ggcggcatcg acgacgtggg tctcggcgtg
     1261 ctcttcgggc ttgatctctg ccgctacgac ttcgtcggcc tgctcatgca cgccgagcat
     1321 cttgaagccg tctggggcgt cggtccgcac acgatctccg tgccgcgcat ctgcccggcc
     1381 gacgacatcg atccggcctc cttcgagaac gccgtgcccg acgacctctt cctcaagatc
     1441 gtcgcgctca tccgcatggc cgtgccctac accggcatga tcatgtcgac ccgcgaaggc
     1501 cagcgcatcc gcaccgaagc cctcaagctc ggcatctccc agatctccgg cggctcccgc
     1561 acgtccgtgg gcggctacgc catccgctcc gttcaggaga tggaggagaa gaccgaacag
     1621 ttcgagacga acgaccgccg ctcgctcgac gaaatcgtcc gctggctcct cgagaaccac
     1681 taccttccgt ccttctgcac ggcttgctac cgcgccggcc gcacgggcga ccgcttcatg
     1741 gaatgggcca aggacggcag catcgccacg tactgccagg ccaacgccat catgacgatg
     1801 aaggagtaca tgtgcgacta cgcctcgaag gaaacccgcg aggccggcga gcgcgtcatc
     1861 gccaacgaac tcccgaagat cccgcacgag aagatccgca tcgccagcga aaagcgcctg
     1921 atccgcatcg ccgacggcga acgcgacttc cgcttctgat cttcaggccc atcgaccttc
     1981 cgatcaattc gcggggactc ctacggaagc cccgcgaccg aacacagggg aaaaaggccc
     2041 tgcggaccac atcggtccgc agggcctttt tgcttctggg gcttcttcag cttccttttc
     2101 cgaggaacgg cccggtcgga ccgggcccgg ccactgcagt catcaatggc ggtagttcac
     2161 cttgctgcag atctcctcga caatcggctc cttgagcctg ttgaactcgg ccttgttctt
     2221 ctcgaagaga ttgccaccat gggcgtcgat cgagacgatg agcggaccga actccttgac
     2281 gcgcatgacc cacatggact cgggctggcc gagttcgggc cagtggcgct cctcgacctc
     2341 ctcgacgcat tcggcggcga gcacggcgca gccgcccggg aagacgcagt gcacggcctt
     2401 gctttcctga cagccggcag cagtctcggg acccatgccg cccttgccga cgacgatctt
     2461 cacgcccgtt ttcctgagga attcgcgctc gtacttctcc atgcgcatgg aggtcgtcgg
     2521 gccgatggag acgacgcgga agccggagcg gctcttgtcg tcgggaacca tgatgggacc
     2581 ggcgtggaag atggccttgc cggcgaggtc gtacggaagg tccatgcctt cctcgacatg
     2641 gcggaagtga accatgtcgc ggctcgtgat gagggcgccg gagagataga acacgtcgcc
     2701 gatcttcagg gactcgatgt cggcgtcgga aaccggtgtc gtcagaactt tcttcgtcat
     2761 tttgctgttc tcccaaatca gagcgtcatg cccttgtggc tgatcatttc gtagctcatg
     2821 tccgcattga tgcggatcgt ggcgcggcgg tggccccagc agcccgtgga aacgcccacg
     2881 gcaaggcagg acggatggcg cgcggcctgt tcgacgttga cgcccatcac ggacttcgtc
     2941 cccgtcaggc cgcccggacc gatgttgatc ttgttgaggc cttcctcgat catcttctcg
     3001 aagtcggcgc cgatctcgtt ggcgttcttc gagcccacgg gacgcatcag ggccttcttg
     3061 gaaagctcgg ccgcaacctc gacgctcccg gcgatgccga tgcccacgag gagcggcggg
     3121 caggcgttga cgccgcgatc gcagatcacg tcgaagatga acttcacgat gcccatgtag
     3181 ccttcggcgg gcatcagcac cttggcggcg ccgggcagcg agcagccgcc gccggccatg
     3241 taaccgtgaa tgaggcagct gtcggagtcg ggaatgatct cccagtcgat ccagggcacg
     3301 cgaacgcccg tgttgttgcc cgtgttgcgc tcgtcgaaga tctggacggc gttctggcga
     3361 agcggcgcct cgatcgtggc gcggcggacg gcctcgtcaa ggcaggcgcg catttcggaa
     3421 agaagcggga agttggcacc cacctcgatg tagtactgga tgacgcccgt gtcctgacag
     3481 agcgggacat tgaggcggcg ggcggcggca aggtcgtcga acatcgcgtc atacacgatg
     3541 cgggagatgt cggaagtctg ctccttgcga agttcggcaa ggcgggtggc aacgtcgtcc
     3601 gggagaatct tgcccagcac ggacgtgaag cgcgtcatga gctcggtgaa gcgctcggcc
     3661 ttttcctgtt gattcattgt gctctcctta agagtctggt tcacgtcctc cgaaaccggg
     3721 gctgtcattc gaaaggacgc gggggttctc atcgcctaaa gcacctgcag ggccggcggt
     3781 ccttcagagc cggataattt cctatatata gaaaaatgtt gtccagcatc aagaccagcc
     3841 tgccttctta acgttcaatg tcgttacaaa gcagtgcttt catcgattta gcacattata
     3901 gaa
//
LOCUS       DBMDTI010000183         3892 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5404_length_3892_cov_2.447112, whole genome shotgun sequence.
ACCESSION   DBMDTI010000183 DBMDTI010000000
VERSION     DBMDTI010000183.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5404_length_3892_cov_2.447112"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(215..1339)
                     /locus_tag="ACZPB6_08035"
     CDS             complement(215..1339)
                     /locus_tag="ACZPB6_08035"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIR1184451.1"
                     /translation="MPHVLPPYRADVVGSFLRPAAVAQARAAFARGELSREALTAVED
                     EEIAALVKKELDNGLTAVTDGEFRRAFWHLDFLAGLKGIRHVKAEAWSVHFEGRQPKA
                     ETVVIEDRLSFPADHPFLEAFDRLKKIAGDAAVKYTIPSPSMLHLICCVRATDYEPIA
                     AYKDNEELLLDDLAQTWTDAMLALYERGCRYLQLDDTSWGEFCSAEKRAAYAARGIDV
                     DAVAHSYVGVLNRIIAAKPADLTLTMHICRGNFRSTWFSSGGYEPIAEILFANCPVDG
                     FFLEYDSERSGGFEPLRFIKDQTVVLGLITSKFPELEDKEAVKARIAEAAKYVPLVQL
                     CLSPQCGFSSTEEGNIMTEEEQWAKVRLVVEIAREVWQDA"
     gene            1552..3468
                     /locus_tag="ACZPB6_08040"
     CDS             1552..3468
                     /locus_tag="ACZPB6_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HIR1184452.1"
                     /translation="MPSLRIQLSALAAAVLGAAAVQAAPSHGGVAAVSSASQTGSGEG
                     DVKIGIMDTISTAVDTASLREGLVLLSNPLEKDFRISWRDIVTPDAAQEVLHQKPDFL
                     MLPTEQIDLMEEQGVQTFRIATRKPWTATDAAHAVGSLVVTRADRTDINTLEDLAGRH
                     IAADTPTSLTGWLALQGEIARAGHDPEKFFGRTTFLSFAYPGVLSAVLHGSADAAVLP
                     TCLLELLDSRGLVNAADLKAIGVKKSDAIVCRHSTALYPDISLVAFPWTPEPLVNRMT
                     VSLLTNGGDANYEWSAMVGTSDLRELYRLLEIGPYRHLRDMSPMAVYKRWQTEIHVGL
                     LVLLLLILNEFRLRGLVAKRTSELSDALSALEASQAEAAVVRDQLGALERKNIAAQMS
                     GMIAHEIKSPVGAIRNFAAVVKILLKDAIAQNPNARTAVEGIDAEALKIAGIVDRVRG
                     YVKNRTANHVPADLKRAVDRGVRAFKLGKLASVPVLVQMPNEPATVLGDGLELELLVL
                     NLVKNAAEAVHEQTDEHGRATGRVIVTLAAIRTETPPEGCGRRQWMLTVADNGRKLTE
                     AEKACLMHSLESVKPDGLGLGLSIVRGIADSHGAKIEFVAKPAGGVVVSVLFDELDEE
                     AAARAAGAIDAGRN"
     gene            3506..>3892
                     /locus_tag="ACZPB6_08045"
     CDS             3506..>3892
                     /locus_tag="ACZPB6_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1184453.1"
                     /translation="MAKVIPIVRIVDDDETFCMSQKMFLQAMGWFVLIYNSAKPFLED
                     DDPSQPGCLILDMRMPEMTGLELQTALVARGAPLPIIFLTGHGDVNMAVHALQHGAFD
                     FLQKPVDPEKLNEVVSRAVEHSIARHE"
BASE COUNT          693 a         1214 c         1300 g          685 t
ORIGIN      
        1 ggttttgaag agcttcctat actatttcca ccatatcctt aattttatca cgtggtagtg
       61 tgaaatgcat gggctgtgaa atgcatgggc ctcaattcct cacctgactg aagtcagatg
      121 tttccttgcg gcgtttctat gaaaaggccg cgtccctgaa tcagggcagc ggccttttgg
      181 agcccgaggg catccgttca ggctggaaag tccgttacgc gtcctgccag acctcgcggg
      241 cgatctcgac cacgagacgg accttggccc actgttcctc ctcggtcatg atgttgcctt
      301 cctcggtcga ggagaagccg cactgcggag agagacagag ctgtacgagc ggaacgtact
      361 tcgctgcttc ggcgatgcgg gccttgacgg cctccttgtc ctcgagttcg gggaacttgg
      421 aggtgatgag gccgaggacg accgtctggt ccttgatgaa gcgcagcggc tcgaatccgc
      481 ccgagcgctc gctgtcgtac tcgaggaaga agccgtcgac ggggcagttc gcgaagagga
      541 tctcggcgat gggttcgtag ccgccggagg agaaccacgt ggagcggaag ttgccgcggc
      601 agatgtgcat cgtgagcgtc aggtcggcgg gcttggcggc gatgatcctg ttgaggacgc
      661 cgacgtagct gtgggccacg gcgtcgacgt cgatgccgcg ggcggcgtag gcggcgcgct
      721 tttcagcgga gcagaattcg ccccagctcg tgtcgtcgag ctgcaggtag cggcagccgc
      781 gctcgtagag cgccagcatg gcgtcggtcc aggtttgtgc gaggtcgtcg agaagaagct
      841 cctcgttgtc cttgtaggcg gcgatcggct catagtccgt ggcgcgcacg cagcagatga
      901 ggtgcagcat cgacggagac gggatcgtgt acttcacggc ggcgtcgccc gcgattttct
      961 tcaggcggtc gaaggcctcg aggaacggat ggtcggcggg gaaggagagg cggtcctcga
     1021 tgacgaccgt ctcggccttg ggctgacgac cttcgaagtg aacggaccag gcctcggcct
     1081 tgacgtggcg gatgcccttg aggccggcaa ggaagtcgag gtgccagaag gcgcggcgga
     1141 attcgccgtc ggtgacggcg gtgaggccgt tgtcgagctc cttcttcacg agcgcggcga
     1201 tctcctcgtc ctcgacggcg gtgagggctt cgcgggagag ctcgcccctg gcgaacgcgg
     1261 cgcgggcctg tgcgacggcg gcgggacgaa ggaagcttcc gacgacgtcg gcacggtacg
     1321 ggggaagaac gtggggcatg gtgcggttcc ttttctaatg tgttgttgga aggcgtcagg
     1381 ggcgtcgagg cggcccgaaa cgacagcggt tcatacaatt tactaccctg aactttcctg
     1441 cggcaaagta atacctcaat aaaacgcgaa tttattcccg ctttcccgag cgtcttgccc
     1501 gaggccgtgc gggcaggcta agattctctg aaccagaccg ccttttccgc catgccgtcc
     1561 cttcgaatcc agctttcagc tctcgccgcc gcagtgctcg gcgctgcggc cgtgcaggcc
     1621 gcgccctccc acggcggcgt tgcggccgtc tcgtccgcat cgcagacggg ctcgggcgaa
     1681 ggcgacgtga agatcggcat catggacacg atcagcacgg ccgtcgacac ggcgagcctg
     1741 agggaaggcc tcgtgctgct ctcgaatccg ctcgagaagg acttccgcat ctcatggcgc
     1801 gacatcgtca cgcctgatgc ggcccaggag gtgcttcatc agaagcccga cttcctgatg
     1861 ctgcccaccg agcagatcga tctgatggag gagcaggggg ttcagacctt ccgcatcgcg
     1921 acccgcaagc cgtggacggc gaccgacgcc gcgcacgcgg tgggctcgct cgtcgtcacc
     1981 cgcgccgaca ggacggacat caacacgctc gaggatctgg cgggcaggca catcgctgcc
     2041 gacacgccga cgagcctcac gggctggctt gcgctgcagg gcgagatcgc ccgcgcgggg
     2101 cacgatccgg agaagttctt cggcaggacc acgtttctta gcttcgccta tcccggcgtt
     2161 ctttccgccg tgcttcacgg cagcgccgac gcggccgtgc tgcccacgtg cctgctcgag
     2221 cttctcgact cgcgtggact cgtcaacgcc gccgacctca aggccatcgg cgtgaagaag
     2281 tcggacgcca tcgtctgccg tcactcgacg gcgctctatc ccgacatcag cctcgtggcc
     2341 ttcccctgga cgcccgagcc cctcgtcaac cgcatgacgg tctcgcttct cacgaacggc
     2401 ggggacgcga actacgagtg gtcggccatg gtgggaacgt cggacttgcg cgaactctac
     2461 aggcttcttg agatcgggcc ctaccgccac ctgcgcgaca tgtcgcccat ggcggtctac
     2521 aagcgctggc agaccgagat ccacgtcggt cttctcgtcc tgcttctgct catcctgaac
     2581 gaattccgcc tgcgcggact cgtcgcgaag cgcacgtcgg agctctccga cgcgctctcg
     2641 gcgcttgagg cgtcgcaggc cgaggccgcg gtcgtgcgcg accagctcgg cgcgcttgaa
     2701 cgcaagaaca tcgccgcgca gatgtcgggc atgatcgctc acgagatcaa gtcgcccgtg
     2761 ggcgcgatcc gcaacttcgc cgccgtggtg aagattcttc taaaggacgc catcgcgcag
     2821 aatcccaatg cgaggacggc cgtcgagggg atcgacgccg aggcgctcaa gattgcgggg
     2881 atcgtcgacc gcgtgagagg ctacgtgaag aaccgcacgg ccaaccacgt cccggcggac
     2941 ctcaagcgtg ccgtcgaccg aggcgttcgt gccttcaagc tcggcaagct cgcctcggtg
     3001 cccgtcctcg tgcagatgcc caacgagccc gcaacggtgc tcggcgacgg actcgagctt
     3061 gagcttctcg ttctcaacct cgtgaagaac gcggccgagg ccgtgcacga gcagacggac
     3121 gagcacggac gcgccacggg ccgcgtcatc gtgacgctcg cggcgatcag gaccgagact
     3181 ccgcccgagg ggtgcggccg caggcagtgg atgctgacgg tggccgacaa cgggcgaaag
     3241 ctcaccgaag ctgagaaggc gtgcctcatg cactcgctcg agagcgtcaa gcccgacggg
     3301 ctcggactcg gcctctcgat cgtgcgcggc attgccgaca gccacggggc gaagatcgag
     3361 ttcgtggcga agcccgcggg cggcgtcgtg gtgagcgtgc tcttcgacga gctcgacgag
     3421 gaggcggccg cgagggccgc gggcgcgatc gacgcgggaa ggaactgaac gaaaagaccg
     3481 gacaaaacaa aggagacgga caacaatggc caaagtcata ccgatcgtgc gaatcgtcga
     3541 cgacgatgaa accttctgca tgtcgcagaa gatgtttctt caggcgatgg gatggttcgt
     3601 gctgatctac aacagcgcga agccctttct tgaggacgac gatccctcgc agccgggctg
     3661 cctcattctc gacatgcgca tgcccgagat gacggggctt gagctgcaga cggcgctcgt
     3721 cgcccgcggc gcgccgctgc cgatcatctt tctcacgggg cacggcgacg tgaacatggc
     3781 cgtgcatgcg ctgcagcacg gggccttcga cttcctgcag aagcccgtcg atcccgagaa
     3841 gctgaacgag gtcgtctcac gcgcggtcga gcattcgatc gcgcgccacg ag
//
LOCUS       DBMDTI010000184         3886 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5416_length_3886_cov_1.984941, whole genome shotgun sequence.
ACCESSION   DBMDTI010000184 DBMDTI010000000
VERSION     DBMDTI010000184.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5416_length_3886_cov_1.984941"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="ACZPB6_08050"
     CDS             <1..1062
                     /locus_tag="ACZPB6_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1184454.1"
                     /translation="SPAPEKSVFLDAVPRDGGIDLLLNAPNTREFAEREVELALRRDE
                     TGTVPDFDVLERAPRMEDAYALLTFTMETVRADDAASSATPDPDDPGRLDTGDVPIRA
                     RDLSRTFGSFVAVADTTFDVKRGEIFGLLGPNGAGKTTTFRMLCGLLAPSGGEIEVVG
                     WDLREAKAEVRAEIGYVAQKFSLYDKLTVEQNLRYFGRSYGFWGPRLTRRVAEAMRGY
                     GLSAYRRMPAKSLPVGAKRDLSIACALLHHPKILFLDEATSGADLASRRAFWRSLTKL
                     AAAGTTIVVTTHFMEEAEYCDRFLIQDAGRILALGSPGEVRAFAAKDAPGRTIRSIED
                     AFIAIVEAARAARKKEAAS"
     gene            1059..2273
                     /locus_tag="ACZPB6_08055"
     CDS             1059..2273
                     /locus_tag="ACZPB6_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1184455.1"
                     /translation="MTAARKTGLLRFSASRCLALIVKEWHQIGRDPSALVIGVFLPLF
                     MLLVCGFGISMDMKDVPVAIVLEERTPVAQRIAVDFTANPYFDAKVFYAKAPAEKALE
                     VREVECILTIPAGFAANAQQGEAAELGLTVYGVDSNSATLFKSYVLGQLNSSVTKMVS
                     NGMIESAVASRVSAGPVKSLASRSWFNEASISTWYLVPGILVIVVGAASTMMSAIVIA
                     REWERGTMAAIFATPASPLEIFLAKWLSYWTIAFGGSLLSLCTSFLVFGQPLRGSIAG
                     VLAILLTLTAMGTALGLFISAKVKNQFLAIELAVVLAYMPSLMLSGFLFDLRSVPVWI
                     EFVGRLLPPTYAVEAFKQCFLAGDGPILWRNVGILCCWAALFFCMAVRVLRKRPPVTV
                     PDKSEPKGGASC"
     gene            2267..3376
                     /locus_tag="ACZPB6_08060"
     CDS             2267..3376
                     /locus_tag="ACZPB6_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1184456.1"
                     /translation="MLKSLWRDVRASLNRIFAIVVKEFAVTFGDKGSRMVLIAPVFLQ
                     AILFGYGATFNLERVPWVFLDESHSSASAEIVRGITGNGIFDLKRNAVSLADFEKSID
                     SSEALVGVYFPNDFDRKGEMMVVSDARNSTTAGIAYGYISSIATQVNAKHGGGLAVEV
                     RDRARWNENGITRYTIVPGLIPVLALIQVLLLAGLSVSREREDGSFDMMLMTPADSIE
                     ILIGKAVMPTLIACGQAFLIFAIGFLWFELPFKGSFLLLGLVIFGFSLCMVGLGLGVS
                     AVADNIQQSIVMIIVMMLPMVILSGLMTSVRAMPHWMQTATILNPLRYSIQAIRAVYF
                     EGAGLMDILPLVWPVALVGLGSMSLATYLFRHKIS"
     gene            3422..>3886
                     /locus_tag="ACZPB6_08065"
     CDS             3422..>3886
                     /locus_tag="ACZPB6_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1184457.1"
                     /translation="MAVKTEDKRRRILSAALDCFEERGFAATRMEDIARSAGVAKGTI
                     YNYFKGKEELLHALAEGIAGVMHRNLENVTSSENEGLPLKDRLMKVVAPLTDGTPRSK
                     RNLRVFRLVWAEGLRSPEIMHDLYARLFVPVFRNDGLLARMLEKEDVPDFVRN"
BASE COUNT          624 a         1337 c         1186 g          739 t
ORIGIN      
        1 tcgcctgccc ccgaaaagtc ggtctttctc gacgccgtcc cccgcgacgg cggcatcgac
       61 ctgctgctca acgcccccaa cacccgcgaa ttcgccgagc gcgaggtcga actcgcgctt
      121 cgcagggacg agacggggac cgttcccgac ttcgacgttc tcgaacgtgc gccgcgcatg
      181 gaggacgcct atgcgctcct caccttcacg atggagaccg tgcgcgccga cgacgccgcc
      241 tcgtccgcaa cgcccgaccc cgacgatccg gggcggcttg atacgggcga cgtgccgatc
      301 cgcgcgcgcg atctttcgcg cacgttcggc agcttcgtgg cggtcgctga cacgaccttt
      361 gacgtgaagc gcggagagat cttcggtctg ctcggcccca acggcgcggg caagaccacg
      421 accttccgca tgctctgcgg ccttcttgcg ccttcgggcg gagagatcga ggtcgtgggc
      481 tgggatcttc gcgaggccaa ggccgaggtg cgcgccgaga tcggctacgt cgcccagaag
      541 ttctccctct acgacaagct cacggtcgag cagaacctgc gctacttcgg gcgaagctac
      601 ggcttctggg gtccgaggct cacccgccgc gtcgccgagg cgatgcgcgg ctacgggctt
      661 tcggcctaca ggcgcatgcc tgccaagtcg ctgcccgtgg gtgcgaagcg cgatttgtcg
      721 atcgcctgcg cgcttcttca ccatccgaag atcctttttc ttgacgaggc gacctcgggc
      781 gccgacctcg cgtcccgccg cgccttctgg cgcagtctca cgaagcttgc ggccgcaggt
      841 acgacgatcg tcgtgacgac tcacttcatg gaggaggcag agtactgcga ccgtttcctc
      901 atccaggacg cgggtcgcat tctggcgctg ggttcgcccg gtgaggtgcg cgccttcgcc
      961 gccaaggacg cgccgggccg cacgatccgc tcgatcgagg acgccttcat cgccatcgtc
     1021 gaagccgccc gagctgcgag gaaaaaggag gccgcatcat gaccgccgcg agaaaaacag
     1081 gactgctgcg cttttcggct tccagatgtc tggcgctcat cgtcaaggag tggcaccaga
     1141 tcgggcgcga tccgtctgcg ctcgtcatcg gcgtcttcct gccgctcttc atgctgctcg
     1201 tctgcggctt cggcatctcg atggacatga aggacgtgcc cgtcgcgatc gtcctcgagg
     1261 agcgcacgcc cgtcgcgcag cgcattgccg tcgacttcac cgcgaatccg tacttcgacg
     1321 cgaaggtctt ctacgcgaag gcgcccgccg aaaaggcgct tgaggtccgc gaggtggagt
     1381 gcatcctcac gattccggcg ggctttgcgg ccaacgccca gcagggcgag gcggccgagc
     1441 tcggcctcac ggtctacggc gtcgacagca attccgcgac gctcttcaag agctacgtgc
     1501 tcggacagct caacagctcc gtcacgaaga tggtatcgaa cggcatgatc gagtccgccg
     1561 tcgcctcgcg cgtctcggct gggcccgtca agtcgctcgc gtcccgcagt tggttcaacg
     1621 aggcgagcat ctcgacctgg tacctcgtac cgggcatcct cgtcatcgtc gtgggcgcag
     1681 cgtccaccat gatgagcgcc atcgtgatcg cgcgcgaatg ggagcgcggc accatggccg
     1741 cgatcttcgc gacgccggcc tcgccccttg agatcttcct cgcgaagtgg ctcagctact
     1801 ggacgatcgc cttcggcggc tcgctcctct cgctctgcac gagcttcctc gtcttcggac
     1861 agccgctcag gggaagcatc gcgggcgtgc tcgccatcct gctcaccctg accgccatgg
     1921 gcacggcgct cggcctcttc atctccgcca aggtgaagaa ccagttcctc gcgatcgagc
     1981 tcgccgtcgt gctcgcctac atgccgagcc tgatgctttc gggcttcctc tttgacctga
     2041 ggagcgtccc tgtctggatc gagttcgtcg gcaggctcct gccgcccacc tacgccgtcg
     2101 aggccttcaa gcagtgcttc ctggcgggcg acggtccgat cctgtggcgc aacgtcggga
     2161 tcctctgctg ctgggccgcg ctcttcttct gcatggccgt gcgggtcctg cgcaagcgcc
     2221 cgcccgtgac ggtccctgac aagtccgaac cgaagggagg cgcatcatgc tgaagtccct
     2281 ttggcgggac gtgcgcgcgt ctctcaaccg catcttcgcc atcgtcgtga aggagttcgc
     2341 cgtcacgttc ggcgacaagg gctcgcgcat ggtgctcatc gcgcctgtgt tcctgcaggc
     2401 cattctcttt ggctacggcg cgaccttcaa ccttgagcgc gtgccctggg tgtttcttga
     2461 cgagtcgcac tcgtcggcct cggccgaaat cgtgcgcggc atcacgggca acggcatctt
     2521 cgacctcaag cgaaacgccg tctcgctcgc cgacttcgag aagagcatcg actcgagtga
     2581 ggcgctcgtc ggggtctact tcccgaacga cttcgaccgc aagggcgaga tgatggtcgt
     2641 ctccgacgcc cgcaactcca cgaccgcggg catcgcctac ggctacatca gctcgatcgc
     2701 gacgcaggtc aacgcgaagc acggcggcgg gctcgccgtc gaggtgcgcg accgcgcgcg
     2761 ttggaatgag aacgggatca cccgctacac gatcgtgcct ggcctcattc ccgtgctcgc
     2821 gctcattcag gtgctgctgc ttgccggtct ctcggtctcg cgcgagcgcg aggacggctc
     2881 gttcgacatg atgctcatga cgcccgccga cagcatcgag atcctgatcg gcaaggccgt
     2941 catgccgacc ctcatcgcct gcggacaggc cttcctgatc ttcgcgatcg gcttcctctg
     3001 gttcgagctt cctttcaagg gctcgttcct gctgctcggc ctcgtgatct ttggattcag
     3061 cctctgcatg gtgggcctgg gtcttggcgt ctcggccgtt gcggacaaca tccagcagtc
     3121 gatcgtcatg atcatcgtga tgatgctccc gatggtgatc ctctcgggcc tcatgacgag
     3181 cgtgcgcgcc atgccccact ggatgcagac cgctacgatc ctcaatccgc ttcgctacag
     3241 catccaggcc atccgcgccg tctacttcga gggcgcgggc ctcatggaca tcctgccgct
     3301 cgtctggccc gtcgctctcg tggggctcgg ctcgatgagc ctcgcaacct atctcttcag
     3361 gcacaagatc tcgtgatctg cgccgaagac aagaaacttt tgaaaaggga gtcaaccgga
     3421 catggccgtc aagactgaag acaagcgccg ccgcatcctt tcggcggccc tcgactgctt
     3481 cgaggaaagg ggctttgcag ccacccgaat ggaagacatc gcccgcagcg cgggcgtcgc
     3541 caaggggacg atctacaact acttcaaggg caaggaggaa ctcctgcacg cgcttgccga
     3601 aggcatcgcg ggcgtcatgc accgcaacct cgaaaacgtg acgagctccg aaaacgaggg
     3661 ccttcccctc aaggataggc tcatgaaggt cgtcgcgccg ctcaccgacg gcacgccgcg
     3721 ctcgaagcgc aacctgcgcg ttttccgact cgtctgggcc gagggcctgc gctcgcccga
     3781 gatcatgcac gacctttacg cgaggctctt cgtgcctgtc ttcagaaatg acggccttct
     3841 tgcccgcatg ctcgagaagg aggacgtccc cgacttcgtg cgcaac
//
LOCUS       DBMDTI010000185         3881 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5423_length_3881_cov_2.408201, whole genome shotgun sequence.
ACCESSION   DBMDTI010000185 DBMDTI010000000
VERSION     DBMDTI010000185.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5423_length_3881_cov_2.408201"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            242..1360
                     /locus_tag="ACZPB6_08070"
     CDS             242..1360
                     /locus_tag="ACZPB6_08070"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR1184458.1"
                     /translation="MQLTVEPKDVSDAYSTPIVQQTSFWSRVKANLGMRSKAFEFSVR
                     NSDLYTDVGGWSRTNADLLMFAQYCSSDEYVAYLPYGPEIEPSEENQGRFLEELSECL
                     RSYLPKGCLAIRYDLNWQSHWCKEGDFDAEGNWRGCPRKEFQEMHMNYGTATWNLFKA
                     NMNVLPSDTIVVDLCGTEEQILASMKPKTRYNIGLARRKGVEVREMGPEALGLWYDLY
                     SQTAHRNGLHVNDRRYFESVIASRMQCSDPEVSVKLLMAFHEEKPLAAMFLILSAHRA
                     TYLYGASATKGRHLMPTYALQWCAMQTARRAGCSEYDLFGVAPNADPSHPMHGLYRFK
                     TGFGGQMYHQMGCWDYPLDMDGYRQFAAREMSMPGYYA"
     gene            complement(1437..1901)
                     /gene="nikR"
                     /locus_tag="ACZPB6_08075"
     CDS             complement(1437..1901)
                     /gene="nikR"
                     /locus_tag="ACZPB6_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HIR1184459.1"
                     /translation="MQRFTLSIDDELAERFDAYLTEHGYANRSECFRDILRNHFTSED
                     VKDNPSAECVGVLSYAYNHHERQLAMRMTEYQHEHAGEVISTMHVHATHEECVESVVI
                     RGRAEDVVTLSRRLSAEPGVRHGRLNLIPVTPDDTHEHAHEHAHSHDHHHGH"
     gene            complement(1932..3524)
                     /locus_tag="ACZPB6_08080"
     CDS             complement(1932..3524)
                     /locus_tag="ACZPB6_08080"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1184460.1"
                     /translation="MHDVFIHNAVIHDGERFTDADALLVKAGRISWIGPGACAPAHDG
                     VPSVDLEGRTVLPALCDVHTHPSWIAATVRSVPCVSPIVNTIEEMIEALRRHPLAGRG
                     PEAWITGFGYDEGKLAENRTPTIEDLDRVSTTQPVFVKRSDCHSAICNSVALRLAGIE
                     AGTPDPAGGRFGRFPDGRPNGVLTEFNAAQVVERLMGEPTFESEVEMMTASGEHFLAR
                     GILAFTEMMADYRQLAVYREAARRGFPVRAGLYLVWKGGHDPRGMPALTDEDRTGDHF
                     IAGVKLFADGSVSGATAAVTTPFLNGSTGMVTLDDGALEAAAEYARTNKIQLSVHAMG
                     DRAVGRIIDFFEDQTPWLEDRPSVRIEHATFLNEAQLARMNASAMRFGVTTQVIFPYA
                     EIEGYRAVLAPKDLERVYPVRTVSSALDAFALSSDAPATAWADPDDPFVSMGAAVTRT
                     AWDGTVFNRSEAVSCEEAVSLYTSRPMKVLPFEGVGRLAPGMRADFIVLRDPVTDDGE
                     ALARNAVSEVFRAGALIHRAPR"
BASE COUNT          706 a         1163 c         1306 g          706 t
ORIGIN      
        1 ttcggggcgt tttcttcatt cgccaatcct gaaccgcagg gaaaagcaga ggatagttgt
       61 ctttacgcaa cacttgggcg cgtgaaggcc ggcattttcc tgcattgaac tgtcgtacta
      121 gtgactttgc ggaaaagatg cgttaaagtg acacctgtac ccgcacgcgg gcgcatcagc
      181 acatgcgccc gcgtgctttt ttgcattctt cgaataaagg aaccaaggga cccttcacgc
      241 catgcagctt accgtcgaac ccaaggacgt ctccgacgcc tacagcacgc ccatcgttca
      301 gcagacctcc ttctggtccc gcgtcaaggc gaatctgggc atgcgttcca aggccttcga
      361 gttttccgtg cgcaacagcg acctctacac cgacgtgggc ggctggtcgc gcaccaatgc
      421 ggacctcctg atgtttgcgc agtactgcag cagtgacgaa tacgtggcct atcttccgta
      481 cggtcccgag atcgagccgt cggaggagaa ccaggggcgc ttcctcgagg agctttccga
      541 gtgcctgagg agctacctgc ccaaggggtg cctggcgatc cgctacgacc tcaactggca
      601 gtcccactgg tgcaaggagg gcgacttcga cgccgagggc aactggcgcg gttgtccgcg
      661 caaggagttc caggagatgc acatgaacta cggcacggcc acctggaacc tcttcaaggc
      721 gaacatgaac gtgctcccct ccgacacgat cgtggtcgat ctgtgcggga ccgaggagca
      781 gatcctcgcc tcgatgaagc ccaagacccg ctacaacatc ggccttgccc gcaggaaggg
      841 cgtcgaagtc cgcgagatgg ggcccgaggc gctcggcctc tggtacgacc tctactcgca
      901 gacggctcac cgcaacggcc tgcacgtcaa cgacaggcgc tacttcgaga gcgtcatcgc
      961 gagccgcatg cagtgttccg atcccgaggt gagcgtgaag ctcctgatgg ccttccatga
     1021 agagaagccc ctcgccgcga tgttcctgat cctctcggct caccgcgcca catatctcta
     1081 cggcgccagt gccaccaagg gccgccacct gatgcccacc tatgcgctcc agtggtgcgc
     1141 catgcagacc gccaggcgcg cgggctgcag tgagtacgac ctcttcggtg tcgcgcccaa
     1201 cgcggatcct tcgcatccga tgcacggcct ctaccgcttc aagacgggct ttggcggcca
     1261 gatgtaccac cagatgggct gctgggacta tccgctcgac atggacggct acaggcagtt
     1321 cgcggcgcgc gagatgagca tgcccggcta ctacgcctga tcgtcaccga caggaacaaa
     1381 aacggcggat ccgcttcgga gtccgccgtt ttgttcatct cgggttcacg tccggctcaa
     1441 tgcccgtggt gatgatcatg tgaatgggcg tgctcatgag catgttcgtg cgtgtcgtcg
     1501 ggcgtcacgg ggatgaggtt gaggcgtccg tggcgcacgc cgggttcggc cgaaaggcgg
     1561 cgggagagcg tgacgacgtc ctcggcgcgg ccgcggatca cgacgctctc gacgcactct
     1621 tcgtgcgtgg cgtgcacgtg catggtggag atgacctcgc ccgcatgctc gtgctggtac
     1681 tcggtcatac gcatggcgag ctggcgctcg tggtggttgt aggcgtagct gagcacgccg
     1741 acgcattcgg ccgagggatt gtccttcacg tcctcactgg tgaagtgatt gcggagaatg
     1801 tcgcggaagc attcggagcg gttggcgtag ccgtgttcgg tcaggtacgc gtcgaagcgc
     1861 tcggcaagct cgtcgtcgat ggaaagcgtg aatcgctgca tgagtgtgtc cccctgagaa
     1921 tgccttccat tttagcgggg ggcgcggtgg atcagggctc cggcacggaa aacctcggag
     1981 acggcgttcc gggcgagagc ctcgccgtcg tccgtgacgg gatcgcgcag gacgatgaag
     2041 tcggccctca tgccgggcgc gagccttcct acgccttcga atggcaggac cttcatcggg
     2101 cgggaggtgt agagcgagac ggcctcttcg caggagacgg cttcggacct gttgaagacg
     2161 gtgccgtccc aggctgtgcg cgtgaccgca gcgcccatcg agacgaaggg atcgtcggga
     2221 tcggcccagg cggtggcggg cgcgtcggag gagagcgcga aggcgtcgag cgccgaggag
     2281 acggtcctca cgggatagac gcgctcaagg tccttcgggg cgagcacggc cctgtagcct
     2341 tcgatttcgg cgtacgggaa gatcacctgg gttgtcactc cgaagcgcat ggccgaggcg
     2401 ttcatgcggg cgagctgagc ctcgttgagg aaggtggcgt gctcgatgcg cacggacggg
     2461 cggtcctcaa gccagggcgt ctgatcctcg aagaagtcga tgatgcgccc gacggccctg
     2521 tcacccatgg cgtggacgga gagctggatt ttgttggtgc gggcgtactc ggccgcggcc
     2581 tcaagcgcgc cgtcgtcgag cgtgaccatc ccggtcgagc cgttgagaaa gggcgtcgtc
     2641 acggccgccg tggcgcccga gacgcttccg tcggcaaaga gcttgacgcc cgcgatgaag
     2701 tggtctcccg tcctgtcctc gtccgtgagc gcgggcatgc cccgagggtc gtgtccgccc
     2761 ttccagacga ggtagagccc tgccctgacg ggaaagccgc ggcgcgccgc ctcgcggtag
     2821 acggcgagct ggcggtagtc ggccatcatt tcggtgaagg cgagaatgcc gcgcgcaaga
     2881 aagtgttcgc ccgaggccgt catcatctcc acttcgctct cgaacgtggg ctcgcccatc
     2941 aggcgctcga cgacctgtgc ggcattgaat tcggtgagaa cgccgttggg gcgaccgtcg
     3001 ggaaagcgtc cgaagcgccc gccggcggga tcgggcgtac ctgcctcaat gccagcgagg
     3061 cgcagggcga cgctgttgca gatcgcagag tggcagtcgg agcgcttcac gaacacgggc
     3121 tgcgtcgtgc tgacgcggtc gaggtcctcg atggtggggg tgcggttttc ggcgagtttg
     3181 ccttcgtcat agccgaagcc cgtgatccag gcctcgggcc ctcggccggc gagcggatgg
     3241 cgcctgagcg cctcaatcat ctcctcgatc gtgttgacga tcggggagac gcagggcacg
     3301 gacctcaccg tggccgcaat ccagctcgga tgggtgtgaa cgtcgcagag cgcgggcaga
     3361 accgtgcgtc cctcgaggtc gacggacggc acgccgtcgt gcgcgggtgc gcaggcgccg
     3421 ggaccgatcc aggagatgcg gcccgccttg acgagaaggg cgtccgcgtc ggtgaagcgt
     3481 tcgccgtcgt gaatgacggc attgtgaatg aagacgtcgt gcatggctct ccggccttga
     3541 agttgaaggg gattcagaaa ggatacggct cgcggaggag gacgtcgccc gaaatctggc
     3601 gggctcttgt gcgccgtcat gcggtatcgg gaaaagagag gaggcggcgt gagggaaagg
     3661 ggaaggaaaa ggcaaaaaaa atggacgttc cgaagaacgt ccattttctc gatgacttgg
     3721 caggggtagt aggattcgaa cctacgaatg tcggattcag aatccgatgc cttaaccgga
     3781 cttggcgata ccccagtaca tctggtgcgg tcggagagac tcgaactctc atggctctca
     3841 ccacagcgac ctcaacgctg cgcgtctacc aatttcgcca c
//
LOCUS       DBMDTI010000186         3823 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5517_length_3823_cov_1.724879, whole genome shotgun sequence.
ACCESSION   DBMDTI010000186 DBMDTI010000000
VERSION     DBMDTI010000186.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5517_length_3823_cov_1.724879"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(491..1129)
                     /locus_tag="ACZPB6_08085"
     CDS             complement(491..1129)
                     /locus_tag="ACZPB6_08085"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HIR1184461.1"
                     /translation="MKTQYAFHFDSSRCTGCKTCVIACRDKNDIQDPKLAFRTVHEET
                     GGSWSRNEDGSWSQNVWAYFVSVSCNHCSKPACVRVCPTGAHARHEELGGLVLIDRDV
                     CIGCGACAVACPYEAPKLDRKAGKMRKCDMCVDRLARKELPVCVAACPQRALDFGTVE
                     ELRKRHGDDGMIAPLAAGDYTKPNLLITKCAQAQPAPERTRSWPDFLNDKTA"
     gene            complement(1150..3594)
                     /locus_tag="ACZPB6_08090"
     CDS             complement(1150..3594)
                     /locus_tag="ACZPB6_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HIR1184462.1"
                     /translation="MTVDLQRRRVFQSAVAGGALSLGAFVPTPVSAAVARAAADIEVM
                     TWSGCAVNCGSRCQLRVFSKNGRVIRIETDASGDASASTIDGGCTQIRACQRGRSMRQ
                     RIYAPERLKFPMKRVGRRGEGRFERISWEQALDEIAARLKDTIDRHTNESVMIMHGSG
                     NQALVNSSAATYRFFNMIGGSLAWYSDYSTACIQNAWPYLYGRFDYGGVRSKAAGQGS
                     YFSQVKNAKLYVTFGNNPAVTRASGGGQSWEFFSAARRNGTRMVFIDPIYSDTMAGRN
                     AEWIPIRPGTDAALCEAIAYVLITENLVDKAFIDRYTIGYDEDSLPASAKPGSSYRSH
                     ILGLGPDKTPKTPERAAGITNVPAERIVRLARDMAAAKPCFISQGWAPQRRMNGETQS
                     TSIAMLPILLGQIGLPGTNSGAREGDSYGLEAGLPTGANPVKVGMPVFLWPQAVVDAA
                     SLTAETAAVRGAPALRHNIKFIWNTQSNTLINQHGGINQLRPILEDETKVETIVCVDT
                     QMTPSAMFADYVLPDVCHQESIDLMADSYAVGDQNYLMLSDKAIDPEWEQKPNWEIMR
                     GLARRFGCEAAYTEGLDLEGWVRRLYAEARKNLPGMPAWDEFRRTGIYKYRMEGDSGI
                     VMEDFRRDPDKHPLETPSGRIEIYSERLAEIARTWKLPEGRGQEIHPIPRYVPTDEMV
                     QVDPKAKQYPLEAFGYHGPGRTHSTYHNVPWLREAHPDVVLMNPVDAQSRGLKTGMLV
                     RVFNDRGALVLPVKVTPRIIPSLVAFPQGAWYRPDEKGLDRGGCFNVLTQLKPTPLAK
                     ANPSHTNLVQVEKFSC"
BASE COUNT          597 a         1171 c         1334 g          721 t
ORIGIN      
        1 cgccatcgag gccacgatgc gcgtcgactt ctaatcgacc gctcacggtt ccgacctgac
       61 gaaacgcccg gtcctcctca cggagggccg ggcgttcttt tcgtccgcaa accgtcatcg
      121 ggacttcgtc tcagacgaga cgacgcgcag cggacctcct gggacggacc ttgaaaaaaa
      181 ggcccgccgt tccttcgcag gatcggcggc cccgaatcgc cggcatttca gccggccagg
      241 aggtcggtac aagaattgtc atctcaggtg cggtcatgtg cacctgttga aatccaaaaa
      301 agcggcccgc cgccccccac acgaggccgg caggcccaaa acgccggccg tcaagccggc
      361 gcatccgagg agacaatcgt ctgctgctgt tcccctgttg ttcgcagccg ttagtttttg
      421 tccgagaaac cgacgtcagg acggttgccc acccgtgacg ccgttcccct gtacgccctg
      481 ttgagccgcc tcaggcggtc ttgtcattga ggaagtcggg ccagcttctc gtgcgctcag
      541 gcgcgggctg ggcctgtgcg cacttcgtga tgagaaggtt gggcttcgtg tagtcgcctg
      601 cggcgagcgg cgcgatcatg ccgtcgtcgc cgtggcgctt cctgagctcc tcgacggtgc
      661 cgaagtccag cgcgcgctgc gggcaggcgg cgacgcagac cggaagctcc ttccttgcaa
      721 gacggtcgac gcacatgtcg cacttgcgca tcttgcccgc cttgcgatcg agcttcggcg
      781 cctcgtaggg acaggcgacg gcgcaggcac cgcagccgat gcagacgtcc ctgtcgatga
      841 ggacgaggcc gccgagctcc tcgtggcggg cgtgggcccc ggtcgggcag acgcgcacgc
      901 aggctggttt cgagcagtgg ttgcaggaga cggagacgaa gtaggcccag acgttctggg
      961 accagctgcc gtcctcattg cggctccagc tgccgcccgt ttcctcatgg acggtcctga
     1021 aggcgagctt cggatcctga atgtcgttct tgtccctgca tgcgatcacg caggtcttgc
     1081 agcccgtgca gcgcgagctg tcgaaatgaa aggcgtattg agtcttcatg gcgtttgagg
     1141 gtccttgggt cagcagctga acttttcaac ttggacgagg ttcgtgtgcg agggattggc
     1201 cttcgcgagc ggcgtgggct tgagctgggt gagcacgttg aagcacccgc ccctgtcgag
     1261 tcccttctcg tcgggcctgt accatgcgcc ctgcgggaag gcaacgaggc tcggaatgat
     1321 cctcggcgtc accttgacgg ggagcacgag tgcgccgcgg tcgttgaaga cgcgaacgag
     1381 catgcccgtc ttgaggcccc tggactgcgc atcgacggga ttcatcagaa cgacgtcggg
     1441 atgcgcttcg cgcaaccacg gcacgttgtg ataggtcgag tgcgtgcggc cgggaccgtg
     1501 gtagccgaag gcctcaagcg gatactgctt cgccttcggg tccacctgaa ccatttcgtc
     1561 cgtcggcacg tagcgcggga tcggatgaat ctcctgtccc ctgccctcgg gcagcttcca
     1621 ggtgcgggcg atctcggcaa gacgttcgga atagatctcg atcctgccgc tcggggtctc
     1681 gagcggatgc ttgtcgggat ccctgcggaa gtcctccatg acgatcccgc tgtcgccctc
     1741 catgcggtac ttgtagatgc cggtcctgcg gaattcgtcc caggcgggca tgccggggag
     1801 gttcttcctc gcctcggcgt aaagccggcg cacccagccc tcgaggtcga gcccctcggt
     1861 gtaggcggcc tcgcagccga agcgtcgggc gagcccgcgc atgatctccc agttgggctt
     1921 ctgctcccac tcgggatcga tcgccttgtc gctgagcatc aggtagttct ggtcgcccac
     1981 ggcataggag tcggccatga ggtcgatcga ctcctgatgg cagacgtcgg ggagcacgta
     2041 gtcggcgaac atggccgagg gcgtcatctg cgtgtcgacg cagacgatcg tctcgacctt
     2101 cgtctcgtcc tcgaggatcg gacggagctg gttgatgccg ccgtgctggt tgatgagcgt
     2161 gttcgactgg gtgttccaga tgaacttgat gttgtgcctg agggccggcg cgccgcgcac
     2221 ggcggcggtt tcggccgtga gcgaggcggc gtcgacgacg gcctgcggcc acaggaagac
     2281 gggcatgccc accttgacgg ggttggcgcc cgtgggcaga ccggcttcaa ggccgtagga
     2341 gtcgccctcg cgggcacccg aattggtgcc gggcaggccg atctggccga gcagaatcgg
     2401 gagcatggcg atgctcgtcg actgcgtttc gccgttcatg cggcgctgcg gcgcccagcc
     2461 ctgcgagatg aagcagggct tcgcggcggc catgtcgcgg gcaaggcgca cgatgcgctc
     2521 tgcgggcacg ttcgtgatgc cggcggcgcg ctcgggcgtc ttcggagtct tgtcgggacc
     2581 gaggccgagg atgtggctgc ggtagctcga gccgggcttg gcggacgcag ggagcgagtc
     2641 ctcgtcgtag ccgatcgtgt agcggtcgat gaaggccttg tcgacgaggt tctcggtgat
     2701 gagcacgtag gcgatcgcct cgcagagggc cgcgtcggtg ccaggacgga tcggaatcca
     2761 ctcggcgttg cgtccggcca ttgtgtcgga gtagatcggg tcgatgaaga ccatgcgcgt
     2821 gccgttcctg cgtgcggcgc tgaagaactc ccaggactgt ccgccgcccg aggcgcgcgt
     2881 gacggcggga ttgttgccga acgtgacgta gagcttcgcg ttcttcacct gcgagaagta
     2941 ggagccctgg cctgcggcct tgctgcgcac gccgccgtag tcgaagcggc cgtagaggta
     3001 cggccaggcg ttctggatgc aggctgtgga gtagtcggag taccaggcaa gcgagccgcc
     3061 gatcatgttg aagaagcggt aggtggccgc gctcgagttg acaagcgcct gattgcccga
     3121 gccgtgcatg atcatgacgg actcgttggt gtgacggtcg atggtgtcct tgaggcgggc
     3181 ggcgatttcg tcgagggcct gctcccagga gatgcgttcg aagcggccct cgccgcggcg
     3241 gccgacgcgc ttcatcggga acttgaggcg ctcgggcgca tagatgcgct ggcgcatgga
     3301 tcggccgcgc tgacaagcgc ggatctgcgt gcagccgccg tcgatggtgc tcgcggaggc
     3361 gtcgcccgag gcgtccgtct cgatgcggat gacgcgtccg ttcttggaga agacgcggag
     3421 ctggcagcgg gagccgcagt tgactgcgca gccgctccag gtcatgacct cgatgtccgc
     3481 ggccgccctg gcaacggcgg cgctcacggg cgtgggaaca aaggcgccga ggctgagggc
     3541 gcctccggca actgctgact gaaagacgcg gcggcgttga agatcgaccg tcatggatca
     3601 cctgcactgt tgaaggtccg gcggacgccc cattgcgtcc gcccctgcgg gccttctccg
     3661 tggcgtggga gattcggagc ggcccttcga tgtgccttca tgataaggag cgcgcatttc
     3721 cgccccgttt ccgcagggac gggattgttt cagggaaata acatccgtga tccgcgccgc
     3781 gtgcaggtcc tcttcttcag aggccgaagt cctcgtcgta gtt
//
LOCUS       DBMDTI010000187         3819 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5523_length_3819_cov_3.841581, whole genome shotgun sequence.
ACCESSION   DBMDTI010000187 DBMDTI010000000
VERSION     DBMDTI010000187.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5523_length_3819_cov_3.841581"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="ACZPB6_08095"
     CDS             complement(<1..565)
                     /locus_tag="ACZPB6_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1184463.1"
                     /translation="MTAEQIQYPDDLYATSEDGKTQAPQPIPSTRKAASVKSAVKAPV
                     TAVAPAAMPEADLPLFTPKAPAATATASALVELCRQYLATPDVEKIREAYRYADAAHL
                     GQFRKSGEPYITHPIAVASILASWRMDAPTIQAGLMHDVLEDTGTSKQEMAEKFGIEV
                     AELVDGVSKLDKLRFSSAAAAQAESFRK"
     gene            complement(738..938)
                     /gene="rpoZ"
                     /locus_tag="ACZPB6_08100"
     CDS             complement(738..938)
                     /gene="rpoZ"
                     /locus_tag="ACZPB6_08100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR1184464.1"
                     /translation="MARITVEDCLKKIPNRFQMVLVAAYRARHLTQGYAPKVEAKDKP
                     AVVALREIAKGEVGLEMLKRVP"
     gene            complement(992..1639)
                     /gene="gmk"
                     /locus_tag="ACZPB6_08105"
     CDS             complement(992..1639)
                     /gene="gmk"
                     /locus_tag="ACZPB6_08105"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR1184465.1"
                     /translation="MSSESAARPLHAGRLFLVTAPSGAGKSSLVNALLKLEPTLKLSI
                     SHTTRQPRPGEVNGREYHFVSEHEFLAMRDRGEFLESALVHGNYYGTSRIWIEGQMAQ
                     GNDVLLEIDWQGARQVRERFDGTVGIFILPPSIEALEWRLHHRGTDSEQTITRRLLGA
                     GAEMSHAPEFEYVIINEDFETALSQMQAVVVASRLAFGQQAVRHREQFASLGIPL"
     gene            complement(1807..2706)
                     /locus_tag="ACZPB6_08110"
     CDS             complement(1807..2706)
                     /locus_tag="ACZPB6_08110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR1184466.1"
                     /translation="MTVASMTGYAVASGATPLGTVTLECRSVNSRFLDLTLRLNEDLR
                     FADSLIRETIQKRIARGKVEIRGYITPDENAAPARINTAVLNRIVELQQTVRDVVPNA
                     RELSVSDLLAMPGVMVNDRIDQDAVSSAVLAVLNNALDAFTASRAREGEALASVLLNN
                     CQQIEEVVTEVKGRMPEILAHIEGKLKERLENALCTALTETSTLTREEVSDRIRQEVT
                     LYAMRMDVEEEINRLYTHVTEVRRILNKGGAVGRRLDFVVQEMNREANTLGSKAAAIE
                     MTNASLTLKVVIEQMREQIQNLE"
     gene            2890..3624
                     /gene="rph"
                     /locus_tag="ACZPB6_08115"
     CDS             2890..3624
                     /gene="rph"
                     /locus_tag="ACZPB6_08115"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR1184467.1"
                     /translation="MTQVQDSARCDGRRPDELRPLGFSRGFTKYAEGSVLVSAGDTRV
                     LCTATVTPGVPRFLTGKGEGWVTAEYGLLPRSTGTRCDREAVKGKQSGRTQEIQRLIG
                     RSLRAAVDRRKLGEFTIQIDCDVLQADGGTRCASISGAWVALRDAVNGMLEKGQITED
                     PILTQVSAVSAGIVDGRPVLDLNYVEDSGSDTDMNVVMTGEGRFVEIQGTAEGAVFSR
                     AELDELLRLAEKGNLEIMALQRAAFE"
     gene            3647..>3819
                     /locus_tag="ACZPB6_08120"
     CDS             3647..>3819
                     /locus_tag="ACZPB6_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HIR1184468.1"
                     /translation="MEKSLVLASNNKGKLREFQAMFAELGVDVINQGALGVEACPEPF
                     GTFIENCLAKARHA"
BASE COUNT          687 a         1006 c         1322 g          804 t
ORIGIN      
        1 tcttgcggaa gctttcggcc tgtgccgcgg cagcggagga gaagcggagc ttgtcgagct
       61 tgctcacgcc gtcgacgagt tcggcgacct cgatgccgaa tttttccgcc atctcctgct
      121 tgctcgtgcc cgtgtcctca aggacgtcgt gcatgaggcc cgcctgaatg gtcggggcgt
      181 ccatgcgcca ggaagcgaga atggaggcga cggcgatcgg gtgggtgatg tagggttcgc
      241 cggactttcg gaactgtccg agatgggcgg cgtcggcgta gcggtaggcc tcgcggatct
      301 tttcgacgtc aggcgtggcg aggtactgtc ggcagagctc gacgagcgcg gaggcggtgg
      361 cggtggccgc aggcgccttg ggcgtgaaga gcggcaggtc ggcctcaggc atggcggcag
      421 gcgccacggc ggtcacggga gccttgacgg cagacttgac ggaggctgcc ttgcgcgttg
      481 aaggaatggg ctgaggggcc tgcgttttgc cgtcctcgct cgtagcgtag aggtcgtcag
      541 gatattgaat ttgctcggct gtcataacgg cagggattcc gtattgatga ggagggcttc
      601 agtcgctgcg ggagtttctt tcgcctcccg tcgcttcgca ctatcgaccg ccctgaatgc
      661 aaaaaggcca ggcgtttcgc ccgctttcgc ggcaaaacgt gcctgacctg atgaaagctt
      721 ggtgcgtaac tgacagttta cggcacgcgc ttcaacattt cgagtccgac ctcgcccttg
      781 gcaatctcgc gcagggcaac gacggcgggc ttgtccttgg cttcaacctt cggggcgtag
      841 ccctgcgtca gatgacgggc gcggtaggca gccacgagaa ccatctggaa gcggttcggg
      901 atcttcttca ggcagtcttc aacagtaata cgggccattt tgtttgctgg atagatggga
      961 aaaacgaaaa atcggcggtt tgacgtgagg gtcagagcgg aatgccgagc gaggcgaact
     1021 gttcgcggtg gcggacggcc tgctgaccga aggcgaggcg gcttgcaaca accacggcct
     1081 gcatctggga aagcgccgtt tcaaaatctt cattgattat aacgtactcg aattcgggcg
     1141 catgcgacat ttcggcacca gcgccgagca gacggcgcgt gatcgtctgt tcggaatccg
     1201 tgccgcggtg gtgaaggcgc cattcgagag cctcgatgga gggcggcagg atgaagatgc
     1261 cgaccgtgcc gtcgaagcgt tcacggacct gacgggcgcc ctgccagtcg atctcgagaa
     1321 gaacgtcgtt gccctgggcc atctggcctt cgatccagat gcggctcgtg ccgtagtagt
     1381 tgccgtggac aagggcggac tcgaggaatt cgccgcggtc gcgcatggcg aggaactcgt
     1441 gttcgctcac gaagtggtat tcgcggccgt tgacttcgcc cggacggggc tggcgggtcg
     1501 tgtgcgagat ggagagcttg agcgtcggct cgagcttgag aagtgcgttg acgagcgacg
     1561 acttgccggc gccggagggc gccgtcacga gaaacagcct gcctgcgtgc agcggacggg
     1621 cagcggattc actggacatt cgtgttctcc atcaatggct gacggcgcgt gtgatgcggc
     1681 gcgcgccgtc ggagacccgc ccgaaggcag atcggaaaaa atgaaaggtc aatattttaa
     1741 gggagacggc gccctctgtg gcgccgtctc cccgtgattt ttttaagctg tctcgcaact
     1801 gaacggttat tcgaggttct ggatctgctc gcgcatctgc tcgatgacga ccttcagcgt
     1861 cagagaggcg ttcgtcattt caatggcggc ggccttggag ccgagggtgt tggcctcgcg
     1921 gttcatttcc tgcacgacga agtcaagtcg gcggccgacg gcgccgccct tattcagaat
     1981 gcgtcggact tcggtcacgt gcgtgtagag tcggttgatt tcctcctcga cgtccatgcg
     2041 catggcgtag agcgtcactt cctgacggat gcggtcgctc acttcctcgc gggtgagcgt
     2101 gctcgtttcg gtaagtgctg tgcagagggc gttttcaagg cgttccttga gcttgccctc
     2161 gatgtgggcg aggatttcgg gcatgcggcc cttgacttcg gttacgactt cttcaatctg
     2221 ctggcagttg ttgaggagca cgctggcgag cgcctcgcct tcgcgggctc gggaggcggt
     2281 gaaggcgtcg agcgcgttgt tgagcacggc aagcacggcg cttgagacgg cgtcctgatc
     2341 gatgcggtcg ttcaccatga cgccgggcat cgcgagaaga tccgaaacgg agagttcacg
     2401 ggcgttgggt acgacatcgc gaacagtttg ctgcagttcg acaatgcggt tcaggacggc
     2461 ggtgttgatg cgggcggggg cggcgttttc atccggagtg atgtagccgc ggatttccac
     2521 cttgccgcgg gcgatgcgct tctggatggt ttcacggatc agagaatcgg caaagcgaag
     2581 gtcctcgttg agtcggagcg tcaggtcaag gaatcgggag ttgacggaac ggcactcgag
     2641 ggtcacggtt ccgagcggcg tagcgcccga cgcgacggca tagccggtca tgctggcaac
     2701 agtcatggga agaactccag gtgaatgagc agtaaatcac agtgcaccgc aggccatgag
     2761 tcaggctgcg gcaggcgccg tcattgtaac tccttttgcc tgaagttcct tttgtcggga
     2821 gttggtttga gaagcgttcg ttgaggtagc ataagggaca gttgttttcg gaattcggag
     2881 tgctttgaca tgactcaagt tcaagattct gcccgttgcg acgggcgtcg tcccgacgag
     2941 cttcgcccgc ttggtttcag ccgtgggttt acgaaatatg cggaaggcag cgtgcttgtt
     3001 tcggcaggtg acacccgcgt gctttgcacg gcgaccgtga cgccgggcgt gccccgcttc
     3061 ctgacgggca agggtgaggg ctgggtgacg gccgagtacg gtctgcttcc gaggtccacg
     3121 ggcacgcgtt gcgatcgcga ggccgtcaag ggcaagcagt caggccgcac gcaggaaatc
     3181 cagcgtctga tcggacgaag ccttcgcgct gcggtcgacc gaaggaagct cggtgaattt
     3241 acgatccaga tcgactgcga cgttctgcag gccgacggcg gcacgcgttg cgcctcgatt
     3301 tcaggtgctt gggttgcgtt gagggatgcc gtcaacggca tgcttgaaaa ggggcagatc
     3361 acggaggacc cgatcctcac tcaggtgagc gccgtgtcgg cgggcattgt tgacggtcgt
     3421 cccgtcctcg atctcaacta tgttgaggac agcggcagcg acaccgacat gaacgtcgtg
     3481 atgacgggcg aggggcgctt cgtcgaaatt caggggacgg cggaaggcgc ggtcttcagt
     3541 cgtgcagagc ttgacgaact gctgcgtctt gccgaaaagg gcaatctgga aatcatggcg
     3601 ctgcagcgtg ctgcgtttga gtaaggttga gtaaggggag atggggatgg aaaagtctct
     3661 ggtgcttgct tccaacaaca agggaaaact tcgtgagttt caggcgatgt tcgccgagct
     3721 cggcgtcgat gtgatcaatc agggcgcgct tggtgtcgaa gcctgtccgg agcccttcgg
     3781 caccttcatt gagaattgtc tcgccaaggc tcgtcatgc
//
LOCUS       DBMDTI010000188         3819 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5524_length_3819_cov_2.349919, whole genome shotgun sequence.
ACCESSION   DBMDTI010000188 DBMDTI010000000
VERSION     DBMDTI010000188.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5524_length_3819_cov_2.349919"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="ACZPB6_08125"
     CDS             complement(<1..806)
                     /locus_tag="ACZPB6_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR1184469.1"
                     /translation="MSASAQPTFAGTRCLPIEDYFHRIPLVPWICPPLHPELLRLLEN
                     IGQESVLAPGAQIYREGEPVSRFAVVKKGLVARKLGTYGAKSDNQIGLAGPGNIATGN
                     LNIFSHRPAVGAYEAILPTSIVWCEHREFFEIARRNPDLFMLLATQCELSMLSDRLAF
                     ACLTLLNSEQAMKVVLLSWTSHFGHLEGDEVIAPRVLTRSIIRSVLGCSMGWVDRLAA
                     EWRRNDVRRVEGGIVHYRLDILQEANEILCDLEEKVSGLPRPRDVRRYWQR"
     gene            complement(1029..1781)
                     /locus_tag="ACZPB6_08130"
     CDS             complement(1029..1781)
                     /locus_tag="ACZPB6_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1184470.1"
                     /translation="MFMKKAFAAVLATAVLVAGAVEAKDLRVASNTTFPPFEFVNTKT
                     QEITGFEMDLIRAMGKQAGYEVKITNMAFDGIIPAVLSGSVDVGASGFSVTEERKKRV
                     LFTEPFYKSGLTVLVAKGNEDKIQGFKDLDGRKLAVQIGTTAAAKAKDIKDAKVQTFN
                     NAGDAILELANGGCEAVINDKPVTDYILTQQPRLAKKVVHLPDMLSADDFAMVVAKDR
                     PELAAELNAALKALKENGEFDKLMMKWFGKAK"
     gene            complement(2100..3056)
                     /locus_tag="ACZPB6_08135"
     CDS             complement(2100..3056)
                     /locus_tag="ACZPB6_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184471.1"
                     /translation="MMLRLSLIAALSAAVLTGCQSVPTADEIDRMQAPDAHASRAKNL
                     MKVFAFDMHDREDAGRGRAVTVTPEEAAAPGVDLLTSGGSGFFNGMLEKSVMAADPLE
                     TVHYFGFVPVTEAADERAVVRHVAAKTVQAVASQFAKEGWRFAWTSPKADLRSFGYMV
                     TQDVFFEKEDTVCRIPAGISTLEPGRGEGCAVTVVVRSRDVSKGAEPDATGFEPVPAW
                     LDPARPHAWRVRSAALGSRMPDGSVFLTPERQRSLALATGRHTGLYAFDDNGVPYVGW
                     EGAVERFAVSPEARAAIEAQARRAAVPMTDKVMDTVKGWWPW"
     gene            3259..>3819
                     /locus_tag="ACZPB6_08140"
     CDS             3259..>3819
                     /locus_tag="ACZPB6_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1184472.1"
                     /translation="MAQEVHGFQTEVTKLLGLLAKSLYSNKEVFLRELVSNASDAVDK
                     LRFLSLTKPELTADDPIFSIRIKADPEAGTLSVIDNGIGMTLAEANDHLGTIAKSGTE
                     AFMAQLTGEEKKDSQLIGQFGVGFYSSFIVADRVTVISRKAGASPEEAVQWESDGNGT
                     FTSGNITREARGTEIILHLEEDEKEVL"
BASE COUNT          682 a         1179 c         1234 g          724 t
ORIGIN      
        1 cgctgccagt atcgtctcac gtcgcgcggt ctcggcagtc cgctcacctt ctcctccaga
       61 tcgcaaagga tctcgttggc ctcctgcagg atgtcgagcc tgtagtgcac gatgccgccc
      121 tcgacgcggc gcacgtcgtt tcgcctccac tcggccgcca ggcggtcgac ccaccccatg
      181 gagcagccca gcacgctgcg gatgatgctg cgggtgagta ctcgcggcgc gatcacctcg
      241 tcgccttcga ggtgcccgaa atggctcgtc cagctcagaa gtacgacctt catcgcctgc
      301 tcggagttca ggagcgtgag acaggcgaag gcaaggcggt cggagagcat tgagagttcg
      361 cactgagtgg cgagcagcat gaagagatcg ggattgcgtc tggcgatttc gaagaactcc
      421 cggtgctcgc accagacgat ggaggtgggc aggatggcct cgtaggcgcc gactgcggga
      481 cggtgcgaga agatgttgag gttgcccgtg gcgatgttgc cgggccccgc gaggccgatc
      541 tgattgtcgg actttgcgcc gtaggtgccg agcttgcgcg cgacgaggcc cttcttgacg
      601 accgcgaaac ggctcacggg ttcgccctcg cggtagatct gcgcgccggg cgcgagcacg
      661 gactcctgcc cgatgttctc aagaagcctc agaagctcgg gatggagcgg cgggcagatc
      721 cacggcacga gcggaatgcg gtggaagtag tcttcaatgg gaaggcatcg ggtgccagca
      781 aaggtcggtt gggcggaggc gctcacggcc gggctccttc tggtcgaaag atccgccttc
      841 acggcgaagg caaggtcttc atctgggtca ttgggcctta ttttagccgc cgcacaagcg
      901 ttttggaaaa agtccgccat cagaacccga aaccgacaaa gccggccgtc tgcgagggac
      961 gaccggcttc gccgggcggc gcatgtacag gcttgcgccg cagctgagaa tggttcgatt
     1021 gacgcgcctt acttcgcctt gccgaaccac ttcatcatga gcttgtcgaa ttcgccgttt
     1081 tccttgaggg ccttgagggc cgcgttgagt tcggcggcga gttcgggacg atccttggcg
     1141 acgaccatgg cgaagtcgtc ggcggagagc atatcgggca ggtgcacgac cttcttggca
     1201 aggcgcggct gctgcgtgag gatgtagtcc gtcacgggct tgtcgttgat gacggcctcg
     1261 cagccgccgt tggcgagctc gaggatggca tcgcccgcat tgttgaacgt ctggaccttg
     1321 gcgtccttga tgtccttggc cttggcggca gccgtcgtgc cgatctgcac ggcgagcttc
     1381 ctgccgtcaa gatccttgaa gccctggatc ttgtcctcgt tgcccttggc gacgagcacc
     1441 gtcaggccgg acttgtagaa cggttccgtg aagagaacgc gcttcttgcg ctcctcggtg
     1501 acggagaagc cggaggcgcc gacgtcgacg gatccggaga gcactgccgg gatgatgccg
     1561 tcaaaggcca tgttcgtgat cttgacctcg tagcccgcct gcttgcccat cgcgcggata
     1621 agatccattt cgaaacccgt gatctcctgc gtcttcgtgt tgacgaattc aaagggcggg
     1681 aaggtggtgt tggaggcgac gcgcaggtcc ttggcttcaa cggcgccggc gacgaggacg
     1741 gcagtggcga ggacggcggc aaaggctttc ttcatgaaca tttcgggttg ctccgttttt
     1801 tcttgtcttt cgggatgatc ttcgaaaatc cgtgcggatt tcgagaggct caaggcgccg
     1861 cctggcgtct tggtggaaag ttattctacc tgtggccctc tgcccggaat ccggggcgga
     1921 ggacttcctt cctcctaggc cgcatgatcg agatcaaacg aaattttggt ttgcctattg
     1981 ccgacaaaag gcctgtgcag gaggacaaaa gcaccggcct tccctcccat gacgcacgaa
     2041 ggccgacacg ccttcgcgcg ccggccttca agtcaaatga gcctgaggga attttggtcc
     2101 taccagggcc accagccctt gacggtgtcc atcaccttgt cggtcatcgg gacggccgcc
     2161 ctgcgggcct gggcctcgat ggccgcacga gcctcggggc tcacggcgaa gcgctcgacg
     2221 gcaccctccc agccgacgta gggcacgccg ttgtcgtcaa aggcgtaaag acccgtgtgc
     2281 ctgcccgtgg cgagcgcgag cgaacgctgg cgctcgggcg tgaggaagac gctgccgtcg
     2341 ggcattctgg agccgagcgc ggcggagcgc acgcgccagg cgtgcggacg ggcgggatca
     2401 agccaggcgg gcacgggctc gaagcccgtg gcgtcgggct cggcgccttt cgagacgtcg
     2461 cggctgcgca cgacgaccgt cacggcgcag ccttcgccac gaccgggttc aagcgtggag
     2521 atcccggcgg gaatcctgca gaccgtgtcc tccttctcga agaagacgtc ctgcgtgacc
     2581 atgtagccga agcttctcag gtcggccttg gggctcgtcc atgcaaagcg ccagccctcc
     2641 ttcgcaaact gcgaagcgac ggcctgcacg gtcttcgctg cgacgtgcct cacgacggcg
     2701 cgctcgtcgg cggcctcggt cacgggcacg aagccgaagt agtggacggt ttcgagcgga
     2761 tccgcggcca tcacgctctt ctcgagcatg ccgttgaaga atccgctgcc gccgctcgtg
     2821 agcaggtcga cgccgggagc ggcggcctct tcgggcgtca ccgtcacggc gcgtccgcga
     2881 cccgcgtcct cgcggtcgtg catgtcgaac gcaaagacct tcatcaggtt cttggcgcgg
     2941 cttgcgtgcg cgtcgggggc ctgcatgcgg tcgatctcgt cggcggtggg cacgctctgg
     3001 cagcccgtga gaacggcggc cgacagggcg gcgatgagag aaaggcggag catcatgatg
     3061 actcgatttc aagggaaatg attttcgact atacaggatt gcggggacgc gcgcgagcgc
     3121 acgtcgcctc cgacgcacga tcgccttcaa tcgcgccctt gaaaatccgc acatcgtccc
     3181 catatttacg tatatcgggc gccgtgacgc ccgcacgcaa tggaaattgc gctgttgata
     3241 ttggagaaaa attcaaaaat ggcacaggaa gtccatggtt ttcagaccga ggtcaccaag
     3301 cttctcggtc ttctcgccaa gtccctttac tcgaacaagg aagtgttcct ccgcgaactc
     3361 gtttccaatg cgtccgacgc cgtcgacaag ctgcgctttc tctcgctcac gaagcctgag
     3421 ctgactgccg atgatccgat cttctcgatc cgcatcaagg ccgatccgga agcaggcacg
     3481 ctctccgtca tcgacaacgg catcggcatg acgcttgccg aggccaacga tcatctcggc
     3541 accatcgcca agtccggcac cgaagccttc atggcccagc tcacgggcga agagaagaag
     3601 gactcccagc tcatcggcca gttcggcgtg ggcttctact cctccttcat cgtcgccgac
     3661 cgcgtgacgg tcatctcacg caaggcgggt gcgagtcccg aggaagccgt gcagtgggag
     3721 tccgacggca acggcacctt cacctccggc aacatcaccc gcgaggcccg cggcaccgag
     3781 atcatccttc atctcgagga ggacgagaag gaggtcctc
//
LOCUS       DBMDTI010000189         3786 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5569_length_3786_cov_2.056988, whole genome shotgun sequence.
ACCESSION   DBMDTI010000189 DBMDTI010000000
VERSION     DBMDTI010000189.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5569_length_3786_cov_2.056988"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /gene="traV"
                     /locus_tag="ACZPB6_08145"
     CDS             <1..767
                     /gene="traV"
                     /locus_tag="ACZPB6_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HIR1184473.1"
                     /translation="NPAMIRNETARLLRRTAPALATLPLALALSGCGTLTGLNADNDF
                     SCPMTPGVSCRSLSDTYEDSVRGRTPDRIERDKAIEAASKGLPEIEGTNGKAADETPA
                     GPAVQTVKADAAQASVAAPAVRAPERRSSPKRLPEVIVTIWIAPWTDDEGDFHEGEMI
                     HARAFDARWAAARRRAESADRGSAVVRLPFERRRSALKHAQTSVPRVRASESDVASSL
                     GDGSRRFIEAQKAALKGTPYDPDVLAEKMKAPEENP"
     gene            767..3430
                     /locus_tag="ACZPB6_08150"
     CDS             767..3430
                     /locus_tag="ACZPB6_08150"
                     /inference="COORDINATES: protein motif:HMM:NF022580.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HIR1184474.1"
                     /translation="MTSSKNPVREFIEILRRIRTGHENLKVFSGIPGFIPGTGERVDA
                     FSTALPWESATPDGLFTLKGDKPENPEGLGFAIEIFPQTGVTSEMEKTLIGLAAPLEA
                     GSVLSITAFASSAVRGIAELMVPEARVPDGFPPLSLEAEGIIDTATNWAAENLVKGAV
                     EQLTPQAPVYPRHWRVWLTVVVPTTKPDDEEVREKVLTARRAISAVLEQAGLFMGVWD
                     ADVLVGTASEILNPQLIRSGDFVRPSANPFEPPSVQVMKADTEVTVEKHRIRFETLDR
                     KSAVHAVGLGVEHYGGHISLALTSQMLGELGRGGHQIPCPFLFTSIIAVLDSASERVK
                     AEGHRMRALQMSHTPVSQISPWYPEKARQWTIAVNSFQTEGGIARVAHQYLLLAPAGL
                     EAEAVHAAQSIARKAGMDVRRTTCLHAQALMGALPMCAGPLLVEDMKRSFRIQRRTLA
                     TGICGSPVMTEWQGTPIRDDRDRRTPLLTLVGRRGQIMHVDPFANASGNYSVTIVGKP
                     GSGKSVVMNQLAFSCLAQGGLVWIIDVGRSYEKTCAVLDGAFLAFDKEHVWDLNPFAI
                     LEALAGDDRSEGIESVVSILGELVSPGRPLPDLERSVLIQLTASAAQMAAMQGRLATL
                     VDLDALLAERAVHDRRLDDLRMQLEPYLNGPLARWFDGSGRPIDFSSRLTVLELEGLS
                     AHPALRQAVLMTLMLWIEKTMSLDRRTVKLVLIDEAWDLMGSGHSGKFIASGFRRARK
                     HKGGFVVATQSLADFFKSETAEAAWSCADTRIYLRQDAECLSSLEAKGQLASDPWFRA
                     ALPSLTTVRGAWSEMIVKVGDAPPAIGRLVLDRFMQVLYSSLPAEVSAVNAWREAGAT
                     MAASVSAVANGAFKPTPEALAALNRNQESDR"
     gene            3427..>3786
                     /locus_tag="ACZPB6_08155"
     CDS             3427..>3786
                     /locus_tag="ACZPB6_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184475.1"
                     /translation="MTEQKNSSEKTEKTDRNEKPASAPKKPSIEPKSARGASTTARRK
                     TAEEADAALQDKIEKARAGAMPERREPAAAGESAGLPPVQTVRAMPQINRKKDETTSD
                     EKAVAKKPSAPVKMPERD"
BASE COUNT          699 a         1269 c         1190 g          628 t
ORIGIN      
        1 ggaatccagc catgattcga aacgagaccg cacgccttct gcgccgcacg gcccctgcac
       61 ttgccacact tccccttgcc ctcgccctct cgggctgcgg gaccctcacg ggcctcaacg
      121 ccgataacga cttcagctgc ccgatgacgc cgggggtctc ctgtcgttcg ctctccgaca
      181 cctacgagga cagcgtccgc ggccgcacgc ccgacaggat cgaacgcgac aaggcaatcg
      241 aggccgcctc gaagggcctt ccagaaattg aaggcacgaa cggaaaggcc gccgacgaaa
      301 cgcctgcggg tcccgccgtc cagacggtga aggccgatgc ggctcaggct tccgtagccg
      361 cacccgccgt acgagcgcct gagcgccgct cttcgcccaa gcgcctcccc gaggtgatcg
      421 tgaccatctg gatcgcgccc tggacggacg acgaaggcga ctttcatgag ggcgagatga
      481 tccacgcccg cgcctttgac gcccgttggg ccgcagcccg gcggcgagcc gaaagcgccg
      541 acaggggctc cgccgtcgtg cgccttccct tcgagcgccg tcgttcggcg ttgaagcacg
      601 ctcagacttc ggtcccgaga gttcgtgcat cagagtcaga cgtcgcctct tcgctcggtg
      661 acggcagccg ccgcttcatc gaagcccaga aggcggccct caagggcacg ccctatgacc
      721 ccgacgtcct cgcggaaaag atgaaggctc ctgaggagaa cccgtgatga cctcctcaaa
      781 gaatcccgtc agggaattca tcgaaatcct tcggcggatc aggacaggac acgagaacct
      841 gaaggtcttc tccgggattc ccggcttcat tccgggtacg ggcgagcgcg tcgacgcatt
      901 ctcaacggcg ctcccctggg agtccgccac gccagacggc ctcttcacgc tgaagggcga
      961 caagcccgaa aaccccgagg gactcggctt tgcgatcgag atctttccgc agacgggcgt
     1021 cacgtccgag atggagaaga cactcatcgg cctcgcggct ccgcttgaag ccggatccgt
     1081 cctctcgatc acggccttcg cgtcgtcggc cgtaaggggc attgcagagc tgatggttcc
     1141 cgaagcacgt gtgcccgacg ggtttccgcc gctgagcctg gaagccgaag ggatcatcga
     1201 cacggccacg aactgggccg ccgaaaacct cgtgaagggt gcggtcgagc agctcacgcc
     1261 ccaggccccc gtgtatccga ggcactggcg agtctggctc acggttgtcg ttccgacgac
     1321 gaagcccgac gacgaggagg tccgtgaaaa ggtcctcacc gcgcgcaggg ccatttccgc
     1381 cgtgctcgag caggcggggc tcttcatggg cgtgtgggat gcggacgtgc tcgtcggcac
     1441 ggcttccgag atcctcaacc cgcagctcat ccgctcgggc gacttcgtgc gtccgtccgc
     1501 gaacccgttc gagcctccgt ccgttcaggt gatgaaggcc gacacggagg tgacggtaga
     1561 gaagcaccgc atccgattcg agacgcttga caggaagtcc gcggttcatg ccgtggggct
     1621 tggggtcgaa cactacggcg ggcacatctc gctcgccctc acaagccaaa tgctcggcga
     1681 gctcggtcgt ggcggccatc agattccgtg ccccttcctc ttcacgagca tcatcgcggt
     1741 tcttgactcc gcctccgagc gcgtcaaggc cgaaggccac cgcatgcgtg cgctgcagat
     1801 gtcgcacacg cccgtctcgc agatcagtcc ctggtatccc gaaaaggcgc gccagtggac
     1861 gattgccgtc aacagcttcc agaccgaagg gggcatcgcg cgcgtggcgc accagtacct
     1921 gctccttgca cctgcgggac ttgaggccga ggccgttcac gccgcccagt ccattgcacg
     1981 caaggcgggc atggacgtga ggcgcacgac ctgccttcat gcgcaggccc tgatgggagc
     2041 ccttccgatg tgtgcgggtc cgcttctcgt cgaggacatg aagaggagct tccgcataca
     2101 gcgcaggact cttgcgaccg gcatctgcgg ctcgcccgtg atgaccgagt ggcagggaac
     2161 gccgattcgc gacgaccgtg accgcaggac tccgcttctc acgctcgtgg gccgtcgcgg
     2221 ccagatcatg cacgtggatc cctttgcgaa cgcgagcggg aactattcgg tgacgatcgt
     2281 gggcaagccc ggttcgggca agtccgtcgt gatgaaccag ctggccttca gctgcctcgc
     2341 gcagggcggg ctcgtctgga tcatcgacgt gggacggtcc tatgaaaaga cctgcgcagt
     2401 gctcgacggg gcctttctcg ccttcgacaa ggagcacgtc tgggatctca atccctttgc
     2461 gatcctcgag gcccttgcgg gcgacgaccg ctccgaaggg atcgaatcgg tggtgtcgat
     2521 tctgggcgaa ctcgtctcgc ccggccgtcc tcttcccgac ctcgagcgca gcgtgctcat
     2581 tcagctcacg gcctcggccg ctcagatggc ggcgatgcag ggaaggcttg cgacccttgt
     2641 cgatctcgat gcgcttcttg ccgaacgcgc cgttcacgac aggcgacttg acgacctgcg
     2701 catgcagctc gagccctacc tcaatggtcc gctcgcgcgc tggttcgacg gctcgggccg
     2761 tccgatcgac ttcagcagtc gccttacggt gctcgagctc gagggcctct ccgctcaccc
     2821 cgcgcttcgt caggcggttc ttatgacgct gatgctctgg atcgaaaaga cgatgtctct
     2881 cgaccgtcgc accgtgaagc tcgtcctcat cgacgaagcc tgggacctga tgggttcggg
     2941 ccacagcggg aagttcatcg cctcgggctt tcgtcgtgcg cgaaagcaca agggcgggtt
     3001 cgtggttgcc acgcagtcgc ttgcagactt cttcaagagc gagacggccg aggccgcctg
     3061 gagctgcgcc gacacgcgca tctatctgag gcaggacgcc gaatgtcttt cgagccttga
     3121 ggcgaagggg cagctcgcga gcgacccatg gttcagggcg gcgttgccgt cactcacgac
     3181 cgttcgcggg gcgtggtccg aaatgatcgt gaaggtgggc gatgcgccgc ccgcgatcgg
     3241 acgcctcgtg cttgaccgct tcatgcaggt tctctactcg tcgctgcccg ccgaagtctc
     3301 cgccgtcaat gcatggcgcg aggcgggtgc aacgatggcc gcttccgtga gcgccgtcgc
     3361 caacggcgca ttcaaaccca cgcccgaagc ccttgcagcc ctcaaccgaa atcaggaatc
     3421 cgaccgatga ccgaacaaaa gaacagcagc gaaaagacgg aaaagaccga caggaacgaa
     3481 aagccggcct ctgctccgaa gaagccctcg atcgagccga agtcggcccg aggtgcaagc
     3541 acgacggcaa gaaggaagac cgcggaggaa gccgacgctg cccttcagga caagatcgag
     3601 aaggcccgtg caggcgcaat gcccgagcgc agggaacccg cggccgctgg agaatccgcc
     3661 ggccttccac ccgtgcagac cgtgcgcgcc atgccgcaga tcaacaggaa gaaggacgag
     3721 acgacgtccg acgagaaggc cgttgcaaag aagccgtccg cccccgtcaa gatgcccgag
     3781 cgtgac
//
LOCUS       DBMDTI010000190         3762 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5614_length_3762_cov_2.069107, whole genome shotgun sequence.
ACCESSION   DBMDTI010000190 DBMDTI010000000
VERSION     DBMDTI010000190.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5614_length_3762_cov_2.069107"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            99..542
                     /locus_tag="ACZPB6_08160"
     CDS             99..542
                     /locus_tag="ACZPB6_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184476.1"
                     /translation="MSNRLPRALLADIAAVISSGDHASALMMLGDESGPEASAAVSSY
                     RAQCAAALGDFDAAERHLRVALDLVERRMTALPADEAARERLAAALTILPPADAPVTP
                     TLEVISPELTAVRLRRMLAAVFMKAGRELDAEMELALLPVEARSL"
     gene            complement(637..1104)
                     /locus_tag="ACZPB6_08165"
     CDS             complement(637..1104)
                     /locus_tag="ACZPB6_08165"
                     /inference="COORDINATES: protein motif:HMM:NF022182.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HIR1184477.1"
                     /translation="MAYLPEVATVLKLAFEHEGWTYGFDAGTGTLDAGFDLDCRIGQT
                     PLYIHLLEDVVLCHAYAPFKVAEEDRRRVMEFVTRANCGLKLGNFEMDLDTGVVCFKT
                     TFCLTGVPLPTRGAMTELVRGALSVLEDYGNALDDVVKGRKTPSEAVREAERN"
     gene            1448..2785
                     /locus_tag="ACZPB6_08170"
     CDS             1448..2785
                     /locus_tag="ACZPB6_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1184478.1"
                     /translation="MQPTYDQTDPTSPAGSIPHWIDPVRETDLVARRRTVHRTPETGW
                     AEFIATARAAEFFTSLGFKVMVGREFIDPIYVRGRSEAEVSESERLAKAAGVSPVLLQ
                     RMGGLTGLCATFDTGHPGKTLAFRVELDALKMNEPVDPDHIPYREGFASERPGLMHAC
                     GHDGHQAVAFELGRFIVANRDRLTGRIRFIFQPGEEGSRGAYPIVQSGLLDDVDTLLC
                     AHIATDLQAGTVVAAPEKFLCTTKIDLHFEGVQAHAGMQPQLGRNALLAAADAALILM
                     ALPRHSDGMTRVNVGTLHAGEGRNVIAAHADMEVEVRGENEAINRELAREAVTRAQGC
                     AMAFGVKMSHKIMGEAVDFVPDDGIVQMITVCARRARHVREVRPSVPLNGSDDATLMI
                     RRVQSQGGRAGYFLVGAGLEASHEHAAVDFDERYLVTLYDIFANLVIGLSGNW"
     gene            3271..>3762
                     /locus_tag="ACZPB6_08175"
     CDS             3271..>3762
                     /locus_tag="ACZPB6_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR1184479.1"
                     /translation="MFKKSLAAVAVLGAFAGSALAADVQLYGLVDLGLNWTQVDKGTT
                     TTDSFGMGSGQNSGSRFGLKGTEDLGNGYKVGFNLENSFKADDGAFDKGSRLFHRESI
                     LFVETPYGELSAGRTGGLDSGVGRYGQMGSGATAMSTGWDNIAKTSKVFLGLGDRMDN
                     TLTY"
BASE COUNT          692 a         1222 c         1163 g          685 t
ORIGIN      
        1 agagcagttc gcgcctgtac gcgtacaatt catgcactgc gcaccacgcg tgcgttttga
       61 ccggccaaga acagagacag agtgaaagga gagaagagat gagcaaccgt ctgccgcgag
      121 cgctgcttgc agacattgcc gccgtgatca gctcgggtga ccacgcgtcc gccctgatga
      181 tgctgggcga cgagtcggga cccgaggctt cggccgccgt ctcctcctac cgcgcccagt
      241 gcgctgcggc cctcggggac ttcgacgccg ccgagcgcca tttgcgcgtt gcgcttgacc
      301 tcgtcgagcg ccgcatgacc gccttgcccg cagacgaggc cgcgcgcgag cgccttgccg
      361 ccgccctcac gattctgccg cccgccgatg cgcctgtgac gccgactctc gaggtcatct
      421 cgcccgagct caccgccgtg cgccttcgcc gcatgctcgc agccgtcttc atgaaggcgg
      481 gacgcgaact tgacgccgag atggagctcg cgctcttgcc cgtcgaggca agaagcctct
      541 gatgaactga agcgacctga aaaacgaaaa aaaagggccg gattgcggaa aaccgcaccg
      601 tccctttgtc atgtctgaga accgctggtt agagcatcaa ttgcgttccg cttcgcgcac
      661 ggcctcggag ggcgtcttcc tgcccttgac gacgtcgtcg agcgcattgc cgtagtcctc
      721 gagcaccgag agcgcgccgc gcacgagttc cgtcatggcg ccgcgggtcg gcaggggcac
      781 gcccgtcagg cagaaggtcg tcttgaagca gacgacgccc gtgtcgaggt ccatttcgaa
      841 gttgccgagc ttgaggccgc agttggcgcg cgtgacgaac tccattacgc ggcggcggtc
      901 ttcttcggcg accttgaagg gcgcataggc gtggcagagc accacgtcct cgagaagatg
      961 gatgtagagc ggggtctggc cgatgcggca gtcaaggtcg aaccctgcgt cgagcgtgcc
     1021 ggtgccggcg tcgaacccgt aggtccagcc ttcatgctcg aaggcgagct tgagaacggt
     1081 ggcgacttcg ggcagatagg ccatgatgga ctccttcatt caatgggtgt ggcgacgaaa
     1141 gagtgtagca gggcgatcct tatccgatgc acggttcctt attgggaatt tctcgtagtt
     1201 ctacgcagaa tgctttttga aggtgatgaa aagagacggg gcggcaagga aaacgactca
     1261 gcttctaggt gaaacgtctt agtagaccat ccgtattttt gcgggtacac tactccctgt
     1321 gcggtatgcc ggcgtccgca aagcggacgg tcaaccccaa attcagatgc ggattgcgta
     1381 gtaatacgca ggagaagccg cagacaacat atcggcatgc ctcagaccaa agcatcaaga
     1441 cattgacatg caacccactt acgatcagac ggatccgacg tcccccgccg gctccattcc
     1501 gcactggatc gatcccgtca gggagaccga cctggtggct cgcaggcgca cggtccacag
     1561 gacgcccgaa acgggctggg ccgaattcat cgcgaccgcc cgcgctgccg aattcttcac
     1621 gtcgctcggc ttcaaggtca tggtcgggcg cgagttcatc gatcccatct acgtgcgcgg
     1681 ccgctcagag gccgaggtga gcgagtctga gcgccttgcc aaggccgcgg gcgtttcgcc
     1741 cgtcctgctg cagcgcatgg gcggcctcac ggggctgtgt gcgacttttg acacggggca
     1801 cccgggcaag accctcgcgt tccgcgtcga gctcgacgcc ctcaagatga acgagcccgt
     1861 cgatcccgac cacattccct acagggaggg cttcgcctcc gagcgtcccg gcctcatgca
     1921 cgcctgcggc catgacggcc atcaggccgt cgccttcgag cttggccgct tcatcgtggc
     1981 gaacagggac cgtctgacgg gccgcatccg cttcatcttc cagccgggcg aagagggctc
     2041 ccgcggcgcc tatccgatcg ttcagtcggg gcttctcgac gacgtggaca cgctgttgtg
     2101 cgcccacatc gcgaccgacc ttcaggcggg caccgtcgtg gccgcccccg agaagttcct
     2161 ctgtaccacg aagatcgacc ttcacttcga gggtgtgcag gcccacgcgg gcatgcagcc
     2221 ccagctcggc cgcaacgcgc ttctggccgc cgccgacgcc gcgctcatcc tcatggccct
     2281 gccgcgccac tccgacggca tgacccgcgt caacgtcggt acgctccatg ccggcgaggg
     2341 tcgcaacgtg atcgcagccc atgcggacat ggaggtcgag gtccgaggcg agaacgaggc
     2401 gatcaaccgc gagctcgccc gcgaggccgt cacacgtgcc cagggctgtg ccatggcctt
     2461 cggcgtcaag atgagccaca agatcatggg cgaggccgtc gacttcgtgc ccgacgacgg
     2521 catcgtgcag atgatcaccg tctgcgcccg ccgcgcgcgc catgtgcgcg aggtccgtcc
     2581 ctcggtgccg ctcaacggct ccgacgacgc caccctgatg attcgccgcg tgcagagcca
     2641 gggcggccgc gcaggctact tcctcgtggg cgcgggcctt gaggcctcgc acgagcatgc
     2701 cgccgtcgac ttcgacgagc gctaccttgt cacgctctac gacatcttcg cgaacctcgt
     2761 gatcggtctt tcgggcaact ggtagcccga agcccgacct cggccctctc atcccggaaa
     2821 accggccccg ccttgcggcg ggcgccggtt ttctgctttc cggcgtgcag gattcgtgcg
     2881 ttctttgttt ttcgttcatg aggccgcagg ggcggactta gcctggcttc aatgcccgga
     2941 aaaagtgctt tcggaggctg tttgtggtgc cgaaacaaca ctgggagtag aggagacacc
     3001 ttatgactta tgagagacag ttaggtcgag gcgcctgaag aacgcgcgat gcgtcgcgtt
     3061 tgacgcgtta atgcgacagt aaaaatttca cacggcctga atgttctctg gaaaaatgcc
     3121 tcacaggtct tgagaaaaga ctggaccacg gtgcaagcgc agtgcaaaag cgtgcgcgcc
     3181 atctcgacgg agcccctcgg gaagcttccg tcgccgctcg cgggccggcc ggtccggccc
     3241 ggagcgaacc caaaaaatga ggaaatgcaa atgttcaaga agtctctcgc tgccgtcgcc
     3301 gttctcggtg cctttgccgg ctctgctctc gctgccgacg ttcagctcta tggtctcgtt
     3361 gacctcggtc tcaactggac gcaggtcgac aagggcacga ccacgaccga cagcttcggc
     3421 atgggttccg gtcagaactc cggctctcgc ttcggcctga agggcacgga agatctcggc
     3481 aacggctaca aggttggctt caaccttgaa aacagcttca aggctgacga cggcgctttc
     3541 gacaagggct cccgcctctt ccaccgcgaa tccatcctgt tcgtcgaaac gccgtacggt
     3601 gaactctccg ccggccgcac gggcggtctt gattccggtg tcggccgcta cggtcagatg
     3661 ggctccggtg ccaccgccat gagcacgggt tgggacaaca tcgcgaagac cagcaaggtc
     3721 ttcctcggtc tcggcgaccg catggacaac acgctcacct ac
//
LOCUS       DBMDTI010000191         3759 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5620_length_3759_cov_2.614270, whole genome shotgun sequence.
ACCESSION   DBMDTI010000191 DBMDTI010000000
VERSION     DBMDTI010000191.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5620_length_3759_cov_2.614270"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /locus_tag="ACZPB6_08180"
     CDS             complement(<1..1633)
                     /locus_tag="ACZPB6_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184480.1"
                     /translation="MLSWAFRHLKAEERDYLTKRLKHVLGKRTLKGSWFEKDKSLVEM
                     LVAGPAHRAGLGSLPLMTAANTEKMHALDYPGLSEDKTPEAFFRRVEAAATHEKSKEA
                     MRAFHEEIDGTPYDRRAFAMLIIYICGFGLSWYAKAFLERYCREFGANSQTTLSISFD
                     PSLYEGKDGDPEGEEASAGEKSEKPSQEQAPAEAVPAGASETEGAREAPAQTGDEVPG
                     VEAAKVAESAAEPASVVPAPVVPVPAEGAKASTVFPRDFSVTVPPLSRGVTRYLGYIR
                     TSGGNFFNFYVLAEWYKDRFGALESEAKQLFPSYGAFNLKSAGRNQIKEGAFYVIDLK
                     PSELMPNFDENGRVRSDFCKFVDFSRITSSKRFFSASDFGIHLVVKPAEDLDRIDFTS
                     TMIPVSVTDSEDLEDEMKVANEFVLLEHKDCFYGPLRLKRDNRGKNYVTFSGTVNKGL
                     VSAFRIKADKVEEVREYVRWEGAGQSVHVGVRCAKTAFMEAVSVDVWSDADLLRRLLE
                     KSAVRAAYIRDRLQSPEAFEDVEPDVTAGRLARARN"
     regulatory      1897..2069
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2227..3690
                     /locus_tag="ACZPB6_08185"
     CDS             2227..3690
                     /locus_tag="ACZPB6_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR1184481.1"
                     /translation="MSDTTTGSNTGLRRDLKARHITMIAVGGSIGTGLFVASGATIAQ
                     AGPGSALLAYSIIGLMVYFLMTSLGELAAYEPVAGSFSTYGRRYVDEGFGFALGWNYC
                     YNWAITVAVDLVAAQLVMGWWFPDVPGTLWSALFLAFIFTLNYISVKGFGETEYWFAL
                     LKVVTVILFICTGFAMIFGILSGDTPAGFENWAIGDAPFVGGLPALIGVAMVVGFSFQ
                     GTELIGIAAGETEHPEKNIPKAVRQVFWRILLFYILSIFVISLIIPYTDPRLLRNDVA
                     DIAVSPFTLVFEHAGLLSAAALMNAVILTSVLSAGNSGLYASTRMLFVLAHQGSAPKI
                     FGKLSASGVPRNALFATTAVAGLCFLTSLFDNQSVYLWLLNTSGMSGFIAWLGIAISH
                     YRFRRGYVMNGYDVNKLPYKSSFFPFGPMFAFALCMIIMLGQNYQAFLAGTIDWAGVV
                     ATYIGIPLFLAIWLGYKLITGCRFVRYSDMEFPEKYR"
BASE COUNT          675 a         1291 c         1055 g          738 t
ORIGIN      
        1 cgtttctggc gcgggcaagg cgaccggccg tcacgtccgg ctccacgtcc tcgaaggcct
       61 cgggagactg gagccggtcc ctgatgtagg cggcacgcac ggcagacttc tcaagaaggc
      121 gcctgagcaa gtccgcgtca ctccagacgt cgaccgaaac cgcctccatg aaggctgtct
      181 tcgcgcagcg cacgcccacg tgcacggact ggccggcgcc ctcccacctg acgtactcgc
      241 gaacctcctc gaccttgtcg gccttgatcc tgaaggcgga cacgagcccc ttgttgacgg
      301 tgccggagaa cgtcacgtag ttcttaccgc ggttgtctct cttcaggcgc agaggaccgt
      361 agaaacagtc cttgtgctcg agcagcacga actcgttggc aaccttcatc tcgtcttcca
      421 gatcctcaga atccgtcacg ctgacgggaa tcatcgtcga ggtgaagtcg atgcggtcca
      481 ggtcctcggc gggcttcacg acaagatgaa tgccgaagtc ggacgcgctg aagaagcgct
      541 tgctgctcgt gatcctcgag aagtcgacga acttgcagaa gtcggaacgc acgcggccgt
      601 tttcgtcgaa gttcggcatc agttccgacg gcttcaggtc gatgacgtag aaggcgcctt
      661 ccttgatctg gttgcgaccc gccgacttca ggttgaaggc accgtaggag gggaaaagct
      721 gcttggcctc cgactcgagc gcgccgaagc ggtccttgta ccactcggcg agaacataga
      781 agttgaagaa gtttccgcca ctcgtgcgga tgtagcccag ataacgggtc acgcctcgcg
      841 acaagggcgg cacggtgacg gagaagtctc gcggaaagac ggtgctcgcc ttcgcccctt
      901 cagcgggcac aggcacgacg ggtgcaggta cgacggaggc aggctcggcg gcagactcgg
      961 caaccttcgc ggcctcgacg ccggggactt cgtcccccgt ctgcgcagga gcctcccgag
     1021 ccccctcagt ctcggacgca ccggcaggga cggcctcagc cggggcctgc tcctgcgagg
     1081 gcttttcgga cttctcgcct gcggacgcct cttcgccttc aggatcgccg tccttcccct
     1141 cgtacaggct cggatcaaag ctgatggaga gagtggtctg cgagttcgcg ccaaactccc
     1201 tgcagtagcg ttcaaggaag gcctttgcgt accacgacag tccgaatccg cagatgtaga
     1261 tgatgagcat ggcaaatgcc ctgcggtcat aaggagtgcc gtcgatctcc tcatgaaagg
     1321 ccctcatcgc ctccttcgac ttctcgtgtg tcgcagcagc ctccacgcgt ctgaaaaagg
     1381 cctcgggcgt cttgtcctca gacagccccg gatagtcaag cgcatgcatc ttctccgtgt
     1441 tggcggctgt catcagcggc agggagccca accctgcacg atgggccggc cccgccacaa
     1501 gcatctcgac aagactcttg tccttctcaa accaggagcc tttcagcgtg cgttttccca
     1561 gaacatgctt gagcctcttg gtgaggtagt ccctctcctc agccttcaaa tgtctgaaag
     1621 cccaactcag catctcagct tctgccagcc atccttcctt gcccggtcca aacatgccca
     1681 tactcttacc tctatatcaa aaaaattgcg aagctcgcgc aatcctgaaa gtattttata
     1741 gagtggtacg agatggtgca tgcttcgcac gggagacggg cgcggcgaat cgggattctg
     1801 gcatggaaaa aaacggccgc ccgcttcacc cggcctgcaa tctgtgtcaa aataacgcct
     1861 ttcacgcttt tcaaggcttc cgccttgagg ggcgccaggc aagatagagg cgcagggact
     1921 gcatcagtag acggccgagg aggtatccga ggacgccgct cgaaagggca gcctgccgaa
     1981 atgacgccgc ggacacgcgg cggccattgg tgacgcggag aacatccgcc tcactcccgc
     2041 cgatccgcgg cggaggagct gtcgctgacc gttcggtcgc ttttcgacag tgcggccggc
     2101 aggcccgcac ccggaaaagt tcgcccggac atctgctgca agcccgcaag gccccgccgg
     2161 ccgctgcagg ttcgctccgt gtcctccctc ctgcgaacgc atttctttcc ggagattcct
     2221 acccacatgt ccgacactac caccggctcc aacaccggac tgcgccgaga cctcaaggcg
     2281 cgtcacatca cgatgatcgc cgtcggcggc tccatcggca cgggactttt cgtggcgtcc
     2341 ggcgcgacga tcgcccaggc cggtcccggc agcgccctgc tcgcctactc gatcatcggc
     2401 ctcatggtct acttcctgat gacgagcctc ggcgaactcg ccgcctacga acccgtggcc
     2461 ggctcgttct ccacctacgg ccgccgctac gtcgacgaag gcttcggctt cgccctcggc
     2521 tggaactact gctacaactg ggccattacg gtcgcagtcg accttgtggc cgcccagctc
     2581 gtcatgggct ggtggttccc cgacgtgccc ggaacactct ggagcgcact tttcctcgcg
     2641 ttcatattca cgctcaacta catatccgta aaaggcttcg gcgagacgga atactggttc
     2701 gcgctcctca aggtcgtaac cgtgatcctc ttcatctgca cgggcttcgc gatgatcttc
     2761 ggaatcctct cgggcgacac gcctgcgggc ttcgagaact gggcgatcgg cgacgcgccc
     2821 ttcgtgggcg gccttcccgc cctgatcggc gtcgccatgg tggtgggctt ctccttccag
     2881 ggaaccgagc tcatcggcat cgccgctggc gaaaccgagc atcccgagaa gaacattccc
     2941 aaggccgtcc gacaggtctt ctggcgaatc ctgctcttct acatcctctc gatcttcgtc
     3001 atcagcctga tcattccgta taccgacccg cgccttctca ggaacgacgt ggccgacatc
     3061 gccgtaagcc ccttcacgct cgtcttcgag cacgcgggcc tgctctcggc cgccgcactc
     3121 atgaacgccg tgatcctcac ctcggtgctc tccgctggca actccggcct ctacgcctcc
     3181 acgcgcatgc tcttcgtgct cgcgcaccag ggttcggccc cgaagatctt cggcaagctc
     3241 tccgcctccg gcgtcccgcg caacgcgctc ttcgccacga cggcggtcgc gggcctgtgc
     3301 ttcctgacgt ccctctttga caaccagtcc gtctacctct ggctgctcaa cacctcgggc
     3361 atgagcggct tcatcgcctg gctcggcatc gccatcagcc actaccgctt ccgccgcggc
     3421 tacgtgatga acggctacga cgtcaacaag ctgccctaca agtcgtcctt cttcccgttc
     3481 ggaccgatgt tcgccttcgc gctctgcatg atcatcatgc tcggccagaa ctaccaggcc
     3541 ttcctcgcgg gcacgatcga ctgggcgggc gtggtcgcca cctacatcgg catcccgctc
     3601 ttcctcgcca tctggctcgg ctacaagctc atcacgggct gccgcttcgt gcgctacagc
     3661 gacatggagt tcccggaaaa gtaccgctag gaaacttcac tgacaacggg cggcccgctt
     3721 ccatcaaagc ggccgcccgc aggcttcact cctcctggc
//
LOCUS       DBMDTI010000192         3757 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5623_length_3757_cov_1.919037, whole genome shotgun sequence.
ACCESSION   DBMDTI010000192 DBMDTI010000000
VERSION     DBMDTI010000192.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5623_length_3757_cov_1.919037"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(69..548)
                     /locus_tag="ACZPB6_08190"
     CDS             complement(69..548)
                     /locus_tag="ACZPB6_08190"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1184482.1"
                     /translation="MATVEPTWPLMRGEALQEYFCISNAVLDHDRLSAGFRRNKLNPL
                     PCCLLGRLAVDKRYQGQGLGGALLVEALKITQGVSRMSGCWALVVQPKGEKEIGFYEH
                     LGFRRCKVGDPPMLFFEIEHFTNKRSPLQTEGRRDCSPSDLNPNRTRLPASSTLPQP"
     gene            complement(678..941)
                     /locus_tag="ACZPB6_08195"
     CDS             complement(678..941)
                     /locus_tag="ACZPB6_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013561.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HIR1184483.1"
                     /translation="MSAQTISLRLPEEEVAILNLLSDRERRTKTQIIREALQPLFRKV
                     LDEPERITLSNTEFQALLDEMATPPGEEVLARRRHLMTYERWK"
     gene            complement(1110..1454)
                     /locus_tag="ACZPB6_08200"
     CDS             complement(1110..1454)
                     /locus_tag="ACZPB6_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA/HybF"
                     /protein_id="HIR1184484.1"
                     /translation="MHEMSIAEGIIEIVERTAGRSGVTRVKEVRIEVGELAGVDISSL
                     EFAWVSVTRGGPAEGSRLVIERPRGEAWCLDCAKTVPLAKYGDPCPVCGGFHLAATGG
                     TEMRVIDFVPED"
     gene            complement(1442..2737)
                     /locus_tag="ACZPB6_08205"
     CDS             complement(1442..2737)
                     /locus_tag="ACZPB6_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184485.1"
                     /translation="MIDAGVITVDLLLALESRPGDLRVRRASLLNERTLNLMDRLARS
                     LGSCREVVPLVFSLCPCAHLVTLDAAERAAAGLAEDERRTVDGCLAERALMLEALLEN
                     IRVLALDASKLVCVPVQADSLAAYAKARAGFSGVIRTLQGFNLVTGQVDEDALLEAHR
                     LIDRLTADCEGLLASLVFGISPEAFLEMAEPVQYAAWYGTNASTVASALAYRYHALPA
                     AFGALDCPPVPQPHELDFPDFADEMYHRLRNEADFEAEPVYRRRPSFTGALVREAGHP
                     LVEALCATAGQSAGTLLAARLLDTAAFWAQLAHREHENLPALPEVYTMRLPDTCGAIA
                     CATTARGLLTHASGFRQSRSGLEHFHAVVSPTEWQFAPEGPGQQALESAVRHLKRSGA
                     GVDEETLRNIIRLALFGLDACVPLDIRLRAAGRSDTCMK"
     gene            complement(2734..3411)
                     /gene="hybE"
                     /locus_tag="ACZPB6_08210"
     CDS             complement(2734..3411)
                     /gene="hybE"
                     /locus_tag="ACZPB6_08210"
                     /inference="COORDINATES: protein motif:HMM:NF023365.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[NiFe]-hydrogenase assembly chaperone HybE"
                     /protein_id="HIR1184486.1"
                     /translation="MEETKVTQAAHEAERKTVRRTMPTQMSRLRVFANSPAPHFESGF
                     GRIAETRMKGLPILNPRIRVKADNFRRWGNDWIGTVTTPWAVIVVYACGCRESWVDVP
                     AGEVRTLELPAGDFPFLGCRDDLLGDYLMLSLVSPVTEFEDQAAAEAVAEIALRTILT
                     VPEDEKFGTNYDTEEEGTPIPPLTPPVAGRKVIPIRAVPQGGADGGSVQVTRRGFFTR
                     MAKGADK"
BASE COUNT          762 a         1131 c         1127 g          737 t
ORIGIN      
        1 ggctgggctg accgtcttgc gctcgtaacg tcacgagcca aagcctgtgg agatgcgtaa
       61 gaccgaagct atggctgagg caacgtcgaa gaagcaggaa gcctcgtccg atttgggttc
      121 aaatcggacg gggagcagtc acgccgccct tccgtttgga ggggcgaccg tttatttgtg
      181 aagtgttcga tttcgaaaaa gagcattggc ggatctccta ctttgcatcg gcgaaagccg
      241 agatgctcat aaaagccaat ttctttttcg cccttaggtt gaacgactaa cgcccaacaa
      301 ccggacatcc tggaaacgcc ctgcgttatc ttcaaggcct cgactaacaa tgctccgccg
      361 agcccttgcc cctgatagcg tttatcgacc gctagtcttc caagcagaca gcacggcaac
      421 gggtttagct tattccgacg aaaacccgct gacaagcgat catgatcaag aacagcattg
      481 ctaatgcaaa aatactcctg caatgcttcc cccctcatca aaggccacgt aggttcgact
      541 gttgccatac ttttcgttat gaacagagcg cgtccgcaac cactggttca ataccgggac
      601 gtcgcaatca aagcgatcta gagcatcttg tggaatgtcc gggtcgagag gcagtggctt
      661 ggaaaaagaa taaacggtca tttccaacgc tcataggtca tgagatggcg acgacgtgca
      721 agaacttctt caccaggcgg cgttgccatt tcatctaaca aggcctgaaa ttccgtattg
      781 cttaacgtaa tcctttccgg ctcatccaag actttcctga aaagaggctg cagagcttcg
      841 cgaataattt gagttttggt gcgccgctct ctgtctgaga gaagattgag aatggcgacc
      901 tcctcttccg gcagacgcag actgatggtt tgagcactca tatggtgtcc tcctctaatc
      961 tatggagata cgataaccca aatcgtattg cattgcaata caagagcgcc tcttcatgcc
     1021 tgcctttcct tttcctgttt ctccaacttg aggggcggtg tcgcttgagc ggtgttgata
     1081 atcgttgttt acgcatgaga ccatagaagt caatcttcgg ggacgaagtc gatgacgcgc
     1141 atttcagtgc ctcccgtggc agcgagatga aagcccccgc agacggggca gggatcgcca
     1201 tatttggcaa gcggaacggt tttggcgcag tcgaggcacc aggcctctcc ccgcggccgt
     1261 tcaatcacga gccttgagcc ctcggcaggc cctccgcgag tgacggagac ccaggcgaat
     1321 tcaagggacg agatgtcaac ccctgcgagt tcgcccacct cgattcgaac ctccttcacg
     1381 cgtgtgacgc cgctccggcc tgccgtgcgc tcgacaatct cgatgatgcc ttcggcaatg
     1441 ctcatttcat gcatgtatca ctccttcctg cagcccgaag cctgatgtca agcggcacgc
     1501 aggcgtcaag cccgaagagc gccagacgga tgatgtttct gagagtctcc tcgtcgacgc
     1561 ccgcaccgga gcgcttcagg tgcctcacgg cgctttcaag cgcctgctgg ccgggaccct
     1621 cgggagcgaa ctgccactcg gtgggggaaa ccacggcatg gaagtgctcg agtccggacc
     1681 tcgactgcct gaagcccgag gcgtgtgtga gaagacctcg ggcggtcgtc gcgcaggcaa
     1741 tcgccccgca ggtgtcggga agtcgcatcg tgtagacctc gggaagggca ggcagattct
     1801 cgtgctccct gtgtgcgagc tgtgcccaga aggcagccgt gtcaagaagg cgtgccgcca
     1861 gaagcgtgcc ggcggactga cccgccgtgg cgcagagggc ttcgacaaga gggtgtcccg
     1921 cctccctcac gagtgctccc gtaaacgagg gcctgcggcg gtaaacgggc tcggcctcga
     1981 agtcggcttc attcctgagc ctgtggtaca tctcgtcggc aaaatccgga aagtcgagct
     2041 catggggttg cggtacaggc gggcagtcca gcgcaccgaa ggctgcgggc agggcatggt
     2101 agcggtaggc gagcgccgag gcgacggtcg aggcattcgt cccgtaccag gcagcgtact
     2161 gcacgggctc ggccatctcc agaaaggctt cgggagaaat gccgaagacc agtgatgcca
     2221 gaaggccctc acagtcggcc gtcagtctgt cgatgaggcg gtgtgcctca aggagggcgt
     2281 cttcatcgac ctgccccgtg acgaggttga acccctgcag cgtcctgatg acgcccgaga
     2341 agcccgcccg ggcctttgca taggcggcaa gcgaatcggc ttgaacaggg acgcagacga
     2401 gcttcgaggc gtcaagcgcg agcacgcgaa tgttttccag aagcgcctca agcatcagcg
     2461 cgcgctcggc gagacagccg tcgaccgtgc ggcgctcgtc ctccgcaaga cctgcggccg
     2521 cgcgttcagc ggcgtcaagc gtgacgagat gcgcgcaggg gcataggctg aagacgagcg
     2581 gcaccacctc acggcagctc cccaggcttc gggcaagcct gtccatgaga ttgagcgtgc
     2641 gttcgttgag aagcgatgcg cgtctcacgc gaaggtcccc gggacgggac tccagcgcga
     2701 gcagcagatc gacagtgatg acaccggcat cgatcatttg tccgctccct tcgccatgcg
     2761 ggtgaagaac ccgcggcgcg tgacctgaac ggacccgccg tctgcgccgc cctgcggcac
     2821 ggccctgatc ggaatgacct tcctgcccgc gacgggcggc gtgagcggag gaatgggcgt
     2881 gccttcctcc tcggtgtcgt aattcgtccc gaacttttcg tcttcgggca cggtgagaat
     2941 ggtcctgagg gcgatctcgg ccacggcctc ggccgccgcc tggtcctcga actccgtaac
     3001 gggactcacg agcgagagca tcagatagtc gccgagcagg tcgtccctgc agccgaggaa
     3061 cggaaagtca cctgcgggca gctcgagcgt gcggacttcg cccgcgggca cgtcgaccca
     3121 gctctcgcga cacccgcagg catagaccac gatgaccgcc cacggcgtcg tcaccgtgcc
     3181 gatccagtcg ttgccccatc ggcggaagtt gtccgccttc acacgaatgc gcggattgag
     3241 gatgggaagc cccttcatgc gggtctcggc aatgcgcccg aaccccgact cgaagtgggg
     3301 cgcgggcgaa ttcgcaaaga cgcgcagacg gctcatctgc gtcggcattg tcctgcgcac
     3361 ggtcttgcgt tcggcttcat gcgcggcctg agtcaccttc gtctcttcca cgtccctctc
     3421 ccgttttttc tgttcgcccg tcgtccgctc tctgatgtgg gaggcgcagg cgttgtttat
     3481 agactggagt ataggaaagg gcgcacgctt catgcatccc ccaagagttg atcagggcct
     3541 agtccaaagt atctagaagg gcccattgcc aaaaaggcag gacatccttc tgaacgcgag
     3601 gcgctcaggc cttgagaagc tcaaggagct cctcaacgtg ggtcttcgcc ttgacgccgt
     3661 agagcgcatg aaggatcctg ccttcgggcg agatcacgaa cgtggagcgt tcgatgccgc
     3721 ggcagagcct gccgtacatc atcttctcct tcatgac
//
LOCUS       DBMDTI010000193         3752 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5635_length_3752_cov_2.656259, whole genome shotgun sequence.
ACCESSION   DBMDTI010000193 DBMDTI010000000
VERSION     DBMDTI010000193.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5635_length_3752_cov_2.656259"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            512..2038
                     /gene="xseA"
                     /locus_tag="ACZPB6_08215"
     CDS             512..2038
                     /gene="xseA"
                     /locus_tag="ACZPB6_08215"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR1184487.1"
                     /translation="MARIRSALGGMLGGVWVSGEVSNLSMPASGHVYLTLKDRDASIR
                     CAYFAGSARRHPARFRTGDRIEVTGAVDVYAKSGDLQIKVTDWRHAGAGALYEAYLRL
                     KARLSAEGLFFEGLKKPLPFFVRRVVVVTSAQAAALQDVRRTIDRRTPWVRLMLSPAY
                     VQGESAPLSLMAALDRADAAGADVILLVRGGGSFEDLSAFNDERLVRKLRSLKTPVIA
                     GVGHESDETLAALAADVCASTPTAAAEHIGPDIHYWRSLLDDFEERMTLAVDRKTDDA
                     GQTLDRLEARMSRQDGFLSRQEGDLVRNGMLLERLAAGSLQGREARLETAGSGLAGIG
                     ALPRRRSVQLEALAQRLEDASEGVLGTRARRLDHFALCLQSPDAVLRARSVRLTSLDK
                     TLELAAERACNAQSRRLDQTRTGLAAAFEQSAYRKDMELRLLERRLPDMERRLDEAAL
                     YLRALGHALAGLDPDKPLKLGYARVELADGTVTAAAEIATGDELTLCFADGSRKAVAK
                     "
     gene            2171..2752
                     /locus_tag="ACZPB6_08220"
     CDS             2171..2752
                     /locus_tag="ACZPB6_08220"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR1184488.1"
                     /translation="MFTLPALPYALDALEPAMSRETLEFHWGKHHQTYVNNLNGLIAG
                     TDFEGKSLEEIIRTSTGGVFNNAAQVFNHTFFWQGLKPQGGGMPEGALLEAINAIWGS
                     YDAFRKAFTASAAGNFGSGWTWLVKNADGTLAIVNTSNAGTPLTGDQKPLLALDVWEH
                     AYYVDYRNARPKFIDAFFDSLVNWEFAQQNFAA"
     gene            2959..3459
                     /locus_tag="ACZPB6_08225"
     CDS             2959..3459
                     /locus_tag="ACZPB6_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184489.1"
                     /translation="MHTTTPSYIVELPLRVNDQQNRFLEKAFEFGRTLYNATLGTALG
                     RLQRMRETQEWRVARDMPKGKARTKAFSAVHKAFGLTEFGLTIIANNHRKASGRKDIG
                     AHEAQSIGKAVWRALQRHMFRKAGRPRFKTFWRGLNSIEGTNNQEIMYKLSTLLRTVD
                     KPEGNG"
     gene            3452..>3752
                     /locus_tag="ACZPB6_08230"
     CDS             3452..>3752
                     /locus_tag="ACZPB6_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184490.1"
                     /translation="MGKFVEHLKRKAESAGCEVIELNAYKLKMSQYDPQTDAYRKKPL
                     KERWHRWGNTGTLVQRDAMSAFLACHATEKGHDRALLLEKWTTAEALLSGSGLCRH"
BASE COUNT          772 a         1054 c         1169 g          757 t
ORIGIN      
        1 cggggccttc cttctcagat gcgacttcgt gactcgccta ctgttgcagg gatgtggaca
       61 accctgccga ttttctttct gtccctattg acttttttat gaaaatctgc atgtccgtcg
      121 gtaccgtcga cgtgagcatt atggtgtttt ccccagaggc tgtgagtagc tctgtggata
      181 agtctgtttt ttgacagtta acatcatgaa ataaaaggta aaagtgccga ctgcctaatt
      241 tttgtgcaaa gtgtggaaat ttcaggcttt gggactcgga ttggcctgcg gggcggatgc
      301 gccggagcgc ctgacgagca cgggcaaagg gtagaatgga cgttcgcaga ctccgacaat
      361 tatccacagg cttgtacaca gggaccttat gcccgattat ccctttcttt ttgatgacga
      421 atgctttagt cgtcccgcct ccgctcgtcc gcccgtaccg cttcgcaggg agccggcccg
      481 atctgaaaac gttctggggg tcggggctgc ggtggctcga atccgcagtg cgctcggcgg
      541 catgctgggc ggtgtctggg tgtcggggga ggtgagcaac ctttcgatgc ctgcgtcggg
      601 gcacgtatat ctgacgctca aggacaggga cgcctccatc agatgcgcgt attttgccgg
      661 cagcgccaga cgtcatcccg caaggtttag aacgggcgac aggatcgagg tgaccggggc
      721 tgtggacgtc tatgccaaaa gcggtgatct gcagatcaag gtgacggact ggcgccatgc
      781 tggtgcgggg gcgctttatg aagcctatct gaggctgaag gcccggcttt ccgcggaggg
      841 actctttttc gaaggcctaa agaagccgct tccctttttc gtgaggcggg tcgtcgtggt
      901 gacgagcgct caggcggcgg cgctgcagga cgtccgaagg acgatcgaca ggcggacgcc
      961 gtgggtgagg ctcatgcttt cgcccgccta tgtgcagggg gagtcggctc ctctgtcgct
     1021 tatggctgca ctcgatcgtg cggatgcggc cggggccgac gtcatcctgc tcgttcgcgg
     1081 cggcggctcc ttcgaggacc tgtccgcttt caatgacgag cgtctggtga ggaagttgag
     1141 aagcctgaag acgcccgtca tcgcgggggt cggtcatgag tccgacgaaa cgctcgcggc
     1201 gcttgcagcc gacgtctgcg cctccacgcc caccgccgct gcggagcaca tcggtccgga
     1261 cattcactac tggcgctcgc ttcttgatga cttcgaagag cgcatgacgc tggccgtgga
     1321 cagaaagacg gatgacgcag gacagacgct cgaccggctc gaggcaagga tgagcaggca
     1381 ggacggcttc ctctcaaggc aggaggggga tcttgtgcgc aacgggatgt tgctcgaacg
     1441 tcttgccgca ggcagcctgc aggggcgcga agcgcgcctc gagaccgcag gttcggggct
     1501 cgcaggcatc ggcgccctgc cccggaggcg cagcgtacag ctcgaagcgc tggcgcagcg
     1561 tctggaggat gcatcggagg gagtgctcgg cacacgggcg cgcaggctcg atcacttcgc
     1621 tctgtgcctg cagtcgccgg acgccgtgct gcgggccaga agcgtcaggc tgacgtctct
     1681 ggacaagacc cttgaactcg cggcggaacg cgcgtgcaac gcacaaagcc ggcgcctgga
     1741 ccagacccgg acaggtctgg cggctgcctt cgagcagtcg gcatacagga aggacatgga
     1801 gctcaggctc ctcgagagga gacttcccga catggagagg cgcctcgacg aggctgcgct
     1861 gtacctgcgg gcgctcggac atgcactcgc aggacttgat cccgacaagc cgctcaagct
     1921 cggctacgca agagtcgaac ttgcggacgg aacggttact gcagccgccg aaatcgcgac
     1981 gggggacgaa ctcacgctct gcttcgctga tggcagcagg aaggccgtgg cgaaatgatc
     2041 ctgcggacaa aagggagcct gttcgtgtga aggttgtatc caaatattag gagcctatat
     2101 tggcagatct tttatgataa acttcattta tcgaaactca gatcccgaca ggttcggggg
     2161 agataccaac atgtttactc tgcctgctct tccttatgcg ctcgacgccc tcgagcccgc
     2221 catgagccgt gaaaccctgg aattccactg gggcaagcat catcagacct acgtcaacaa
     2281 tctgaacggc ctgatcgccg gcacggactt cgaaggcaag tcccttgagg aaatcatccg
     2341 cacctccacg ggcggcgtgt tcaacaatgc ggcccaggtc ttcaaccaca cgttcttctg
     2401 gcagggcctc aagccgcagg gcggcggcat gcccgaaggc gcccttcttg aggcgatcaa
     2461 tgcaatctgg ggttcctatg acgccttccg caaggccttt acggcctccg ctgcgggcaa
     2521 cttcggttcc ggctggacgt ggctcgtgaa gaatgccgac ggcacgctgg ccatcgtcaa
     2581 cacgagcaac gccggcacgc cgctcacggg cgatcagaag ccgcttctcg cgctggacgt
     2641 ctgggagcac gcctactacg tcgactaccg caacgcccgt ccgaagttca tcgatgcgtt
     2701 ctttgacagc ctcgtgaact gggagttcgc tcagcagaac ttcgccgcat aaggacgttc
     2761 gattcatgat gctcggaagg gcgccttctc aggaaggcgc ccttcctgcg cttcagggga
     2821 cgggactttg ggcgaggtgt gctaatgtct cgtacatagt cggtctcctt ttgcatgaca
     2881 gaacccgtgc gccgcaatcc tctcattcaa tgggaggtaa ggcgcacaaa gttttcgaac
     2941 gtgaagtaac attgccttat gcataccacc accccttcct acattgtcga acttccgctt
     3001 cgtgtgaacg atcagcagaa tcgttttctc gaaaaggcgt ttgagttcgg acgcacgctc
     3061 tacaatgcga cgctcgggac agctctcgga cgtttgcagc gcatgcgtga aacgcaggag
     3121 tggcgggtgg cacgggacat gcccaagggg aaagcacgca cgaaagcgtt cagtgctgtt
     3181 cacaaagcct ttggactaac cgagttcggc cttacgatca ttgcgaataa tcatcgcaag
     3241 gcgagcggtc gaaaagacat tggggctcat gaagcgcaaa gcattggtaa agcggtctgg
     3301 agggctctcc agcgtcacat gttccggaaa gccggcagac ctcgcttcaa aaccttctgg
     3361 cgtggactca attccatcga aggcacgaac aatcaggaaa tcatgtacaa gctttcaacg
     3421 ctgcttcgga ctgtcgacaa accggagggg aatgggtaag tttgtggagc atctcaaacg
     3481 caaggctgaa agcgccggct gcgaggtgat cgaactcaat gcctataagc tgaagatgtc
     3541 gcagtacgat ccgcaaacgg atgcgtaccg taagaagccg ttgaaggaaa ggtggcaccg
     3601 ctggggaaac accggcacgc tcgtgcagcg agacgccatg agtgcgtttc tggcgtgcca
     3661 tgcaactgaa aaaggacacg accgagccct tctccttgag aagtggacga ctgcggaagc
     3721 actgttgagc ggcagcggtc tgtgtcgtca tg
//
LOCUS       DBMDTI010000194         3706 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5716_length_3706_cov_2.855201, whole genome shotgun sequence.
ACCESSION   DBMDTI010000194 DBMDTI010000000
VERSION     DBMDTI010000194.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5716_length_3706_cov_2.855201"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            110..601
                     /locus_tag="ACZPB6_08235"
     CDS             110..601
                     /locus_tag="ACZPB6_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184491.1"
                     /translation="MRAAVLSALLVPVFLTGCSTYVTVVSEPEGAVITDASGSIVYGR
                     APLDVEYDTNELRHGAHPGQCAQIPGFTARWPSGASASTDAALPLCDLRHGMTVRINR
                     PKSAPGLEQDLQWALERAQKRARDAEIERDRMMLYMDHGWFWGPGWGPWPRTAPPPPR
                     PVR"
     gene            635..1363
                     /locus_tag="ACZPB6_08240"
     CDS             635..1363
                     /locus_tag="ACZPB6_08240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1184492.1"
                     /translation="MLKGKKAVIFDLDGTLIDSLGIWSAVDELLATSLGRPDLAADPV
                     TLARLREDALVRHALEPNPYVGFCADLAAFCGSPLPGEEVHRERYHISRALLKSDVRL
                     KPGAAAFVKTLSERGFKLAVATTTKRANIDIYETVNPHIAPELAFSSVFDAILTRENV
                     SRIKPDPEVYHLALETLGVRADEALVFEDSLPGLRAAHAAGIQTVVVRDPWSEADRAR
                     LLEEADAHVDSLCDAPEICGFTRS"
     gene            1439..2248
                     /locus_tag="ACZPB6_08245"
     CDS             1439..2248
                     /locus_tag="ACZPB6_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012791.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR1184493.1"
                     /translation="MRILLPVDGSEHCRNALRFIASRQALSPEKEATVELLNVQHSIP
                     EAVINALGLDSVRKASQDEGMKVIDAMREDAGDLDVRAKVEGGEFAPVIVREADHMDA
                     DLIVMGCRGLSAMEGFFVGSVSAKVISLTKRPLLLVRDKIPAGAMRVGICVDHSDYAH
                     AAAEFVVNQREFFGKDAVFEVINVSETEETFHQAVEPVMTVFEDAGVPAERVHLKGKV
                     GDAICNYAGEGKLDMLVIGSHGYGNFRAAFMGSTAMRITAGCEVPLLVLKA"
     gene            complement(2238..2732)
                     /locus_tag="ACZPB6_08250"
     CDS             complement(2238..2732)
                     /locus_tag="ACZPB6_08250"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF016120.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine
                     O-acetyltransferase/O-succinyltransferase family protein"
                     /protein_id="HIR1184494.1"
                     /translation="MTRHELGTVCVQGGWRPKNGEPCQLVGIGKTLLERKLSGVFEHR
                     VVRRSSMLFRGCDDVFRIPHSRHTTIDEEAVRAHPELKVLAESDEAGIFALSTEGGRQ
                     IFFTGHAEYDAGILNAEYRRDRAAGLFVRMRQKSAGSPEGAGTLALRCCARRDSGAQR
                     SFRP"
     gene            complement(2896..>3706)
                     /locus_tag="ACZPB6_08255"
     CDS             complement(2896..>3706)
                     /locus_tag="ACZPB6_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR1184495.1"
                     /translation="RSSVEFYQPLGPGSDWFVNPSFEYSVSPFNVYENGEAIARYKNE
                     TSTAQIGLGYSIERLGYARVAAGYFSQASTRTIGVTDISTPEVESPFVSAELLLDTLD
                     SVNFPTKGYRFALQGQRIFDTDGPGYKNIYEANVYVPITYGRWTTLFSGKLGRSAVPS
                     VFSLGGAFELTGSPYGRWTGSDIQLGSVRISRNISDWLGAGERTVWLGASAEAGRAYN
                     RDEAGESGNRDWHRAVGGYVGVDSIIGPLYLMAGRTMDEGWGVYFFWGRKM"
BASE COUNT          648 a         1285 c         1119 g          654 t
ORIGIN      
        1 cctttggcgc gaatcacctc tcccgcaggc acctccgtga gccggcgtac gccccgcatc
       61 acgtgcggtt agaatgacct tctgaacctt tcattttccg gagggcacca tgcgcgccgc
      121 cgttctttcc gctctgctcg ttccagtctt cctcacgggc tgttctacct acgtcacggt
      181 cgtctccgaa cccgagggcg ccgtcatcac cgacgcatcg ggaagcatcg tctacggccg
      241 cgcgcccctc gacgtcgagt acgacaccaa cgagctcagg cacggtgccc atcccggaca
      301 gtgtgcgcag attcccggct ttacggcaag gtggccgagc ggcgcctccg cctcgaccga
      361 cgcagcgctg ccgttgtgcg acctgcgaca cggcatgacc gtgagaatca accgtcccaa
      421 gtccgcacca ggcctcgagc aggacctcca gtgggcgctc gagcgcgccc agaagcgtgc
      481 gcgcgacgca gaaatcgaac gagaccgcat gatgctctac atggaccacg gctggttctg
      541 gggaccgggc tggggccctt ggccgcgcac ggcgcccccg ccgccgaggc ccgtgagata
      601 atttcacttt tgcgcaacac gatcgggaca catcatgctc aaaggcaaaa aggcggtcat
      661 cttcgacctt gacggcaccc tcattgactc tctcggcatc tggagcgcag tcgacgagct
      721 tctcgccacg agcctcggcc gtcccgatct cgccgccgac cccgtgacgc tcgcccgcct
      781 tcgtgaggat gcgctcgtgc ggcatgccct cgaacccaat ccttatgtcg gcttctgcgc
      841 ggatctggcc gccttctgcg ggagcccgct tcccggcgag gaggtccatc gcgagcgcta
      901 ccacatctcg cgcgctcttc tcaagtccga cgtgcgcctc aagccgggcg cggccgcctt
      961 cgtgaagacg ctctcggagc gcggcttcaa actcgccgtc gccaccacga ccaagcgcgc
     1021 caacatcgac atctacgaga cggtcaaccc gcacatcgcg cccgagcttg cattcagcag
     1081 cgtcttcgac gccattctca cgcgcgagaa cgtgagccgc atcaagccag accccgaggt
     1141 ctaccacctc gcgctcgaga ccctcggcgt gcgcgccgat gaggcgctcg tcttcgagga
     1201 ctcgcttccc ggcctgcgtg ccgcgcacgc cgcaggcatt cagaccgtcg tcgtcaggga
     1261 tccgtggtcc gaagccgaca gggcacgact cctcgaggag gcggacgccc atgttgatag
     1321 cctttgcgac gcacccgaaa tctgcggttt tacccgctcc tagccaccct tcgggcgaac
     1381 atcctgcgct agaatggatt caggccaatg gccttcaaac aaacaggagg tatccttcat
     1441 gcgcattctt cttcccgtcg acggcagcga acactgccgc aacgcactcc gcttcattgc
     1501 ctcccgccag gcgctctcgc ctgaaaagga ggcgaccgtt gagcttctca atgttcagca
     1561 ctccatcccc gaagccgtga tcaacgcgct cggcctcgat tccgtccgca aggccagcca
     1621 ggacgaaggc atgaaggtca tcgacgccat gcgcgaagac gccggcgacc ttgacgtccg
     1681 cgccaaggtc gagggcggcg agttcgcgcc cgtcatcgtc cgcgaggccg accacatgga
     1741 cgccgacctc atcgtcatgg gctgccgcgg cctctccgcc atggaaggct tcttcgtggg
     1801 cagcgtctcc gccaaggtga tcagcctcac gaagcgtccg cttctgctcg ttcgcgacaa
     1861 gatccccgca ggcgccatgc gcgtcggcat ctgcgtcgac cactccgact acgcccacgc
     1921 cgccgccgaa ttcgtcgtca accagcgcga gttcttcggc aaggacgccg tctttgaagt
     1981 gatcaacgtc tccgaaaccg aagagacctt ccatcaggcc gtcgaacccg tcatgaccgt
     2041 cttcgaggac gccggcgttc ccgccgaacg cgtgcacctc aagggcaagg tgggtgatgc
     2101 gatctgcaac tacgcgggcg aaggcaagct cgacatgctc gtcatcggct cccacggcta
     2161 cggcaacttc cgggccgcct tcatgggctc caccgccatg cgcatcaccg ccggctgcga
     2221 agtgcccctg ctcgtcctca aggcctgaaa gatctctgcg cgcctgaatc acgacgcgcg
     2281 cagcatctca aagccaatgt gccggctccc tcgggagagc cggcactttt ttgtctcatc
     2341 cgcacgaaaa ggcccgccgc cctgtcccgc ctgtactcgg cgttgaggat gcccgcatcg
     2401 tattcggcat gtccggtgaa gaagatctgc ctgccgcctt cggtcgagag ggcgaagatc
     2461 cccgcctcgt cggattcagc gagcaccttg agctcgggat gtgcgcgcac ggcctcttcg
     2521 tcgatcgtcg tgtgcctcga gtgcggaatc ctgaagacgt cgtcgcaacc cctgaagagc
     2581 atggagcttc gcctcacgac gcggtgctcg aagacacccg agagcttgcg ctcgagaagc
     2641 gtcttcccta tcccgacgag ctggcagggc tcgccgttct tcgggcgcca accgccctgc
     2701 acgcagacgg tgcccagttc gtggcgtgtc acacaaactc ctctgtgtca ggcgttttga
     2761 agaaaacatc ctgtcagact atcagagcga aaggggtggt ttcgaaaaac cgcccctgtc
     2821 ttgggggtat aacccgcgcc tctagtgcat gatcccgaga cgcggccctg acgatgccgt
     2881 ctttgtgcga cggacttaca tcttgcggcc ccagaagaag tagacgcccc agccttcgtc
     2941 catcgtgcgg ccggccatca ggtagagggg gccgatgatg ctgtcgacgc ccacgtagcc
     3001 gcccacggcg cggtgccagt cgcggttgcc cgactcgcct gcctcgtcgc ggttgtaggc
     3061 gcggcccgct tcggccgatg cgccgagcca gaccgtgcgc tcgcctgcgc ccagccagtc
     3121 ggagatgttg cgcgagatgc gcacggagcc gagctggatg tcggagcccg tccagcggcc
     3181 gtagggactg ccggtgagct cgaaggcgcc gcccagcgag aagacgctcg gcacggcgga
     3241 gcggcccagc ttgcccgaga agagcgtcgt ccagcggccg taggtgatcg gcacgtagac
     3301 gttggcctca tagatgttct tgtagccggg accgtccgtg tcgaagatgc gctggccctg
     3361 cagggcgaag cggtagccct tggtcgggaa gttgacgctg tcgagcgtgt cgagcagaag
     3421 ctcggcgctc acgaacggag actcgacctc tggcgtgctg atgtcggtca cgccgatcgt
     3481 gcgggttgag gcctgggaga agtagcccgc ggccacgcgc gcatagccca gacgctcgat
     3541 cgagtagccg agcccgatct gggccgtgct ggtctcgttc ttgtagcgcg cgatggcctc
     3601 accgttttcg tagacgttga agggcgagac cgagtactcg aagctcggat tgacgaacca
     3661 gtcggagccc gggccgaggg gctggtagaa ctcgacgctg gacctg
//
LOCUS       DBMDTI010000195         3701 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5728_length_3701_cov_2.777500, whole genome shotgun sequence.
ACCESSION   DBMDTI010000195 DBMDTI010000000
VERSION     DBMDTI010000195.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5728_length_3701_cov_2.777500"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2041)
                     /locus_tag="ACZPB6_08260"
     CDS             complement(<1..2041)
                     /locus_tag="ACZPB6_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184496.1"
                     /translation="MKASTPIGPSLGLNAVRLSLLSGAVTAALGAPAMAQESMEIELD
                     PFEHTVFVPGEDTRDVLVTGVKTATITAGFNNVLIAPRVEQETTFRLQGFGVSNALTG
                     VSLVLPAGKGPVNVLASGGTTTIVMDTSNLPEDDGQDGGSGNENPDEDLGDLGGFRLL
                     SDENQADYSDGQFKAGAKLVHAFANGGRAALETVGRNPQDVLVIAGHLHVTPDVQMSF
                     GVEGSEETNANFHLGPSAGVVINKSWFMGLAEDKGAITFGKGTKAVFEKGAVFIITDG
                     MDDLREPEEFEADDEAQEVPVEERLVFKAVAGSEVTGVEHLEGFLTKDGQVCSMKFEQ
                     TEDGWRVVEREWRYEGPLEEVVNALGVEAMKADAPKALRKEFLRRQSPSEFGKTINWM
                     TELMVGAGTSTEMIRTAENVVELTSRAAVAFEDDQAVRVSARTGSSRGRFKSVDGSTG
                     GFAEWSRDVTGLDLEVHLRHLTRFGGLVVSYADADVGSSFDVFDVSQESNIASLAVYY
                     GESSDRFAVTGYGMFSGANDKVRVISTGDMLTTGDPSRRAMTAGVAGSFYPDWGGYKD
                     VNFTGAVSLTNYLKSTYGIAFDGERVMDVEEESRWVGTLQLQAGWHRLFRPASSSWIK
                     ADVTLGGRVRAGDLEVRQTVSAGGTSSSVGHDDLTRGEIFGDAALKVRLRDSVAGL"
     gene            complement(2380..>3701)
                     /locus_tag="ACZPB6_08265"
     CDS             complement(2380..>3701)
                     /locus_tag="ACZPB6_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HIR1184497.1"
                     /translation="LDVRTVTMGISLFDCAAPDFDTFAYKVRSKILRNAEKLVETCDI
                     VGDRYGIPVVNKRISVSPMATVCAGFTRDQMVEACRVLDDCAKDVGVDFIGGFGALVE
                     KGMTPGERNLIDALPEALATTDRICSSINVGSTKAGINMDAVKLMGERILDVAAATAD
                     RDAIGCAKLVVFCNIPQDVPFMAGAYLGVGEPDVVINVGVSGPGVVKKALDRAFEAKG
                     DYLTITDAAEVIKHTAYKVTRVGEIIGNEVAQRLNLPFGVADLSLAPTPAVGDSVGEI
                     FQTMGLSSIGAPGTTAILAMLNDAVKKGGVFASSHVGGLSGAFIPVSEDSAIEAAARS
                     GALTMEKLEAMTSVCSVGLDMIAIPGDTSAATISGMIADEMAIGMINSKTTAVRIIPV
                     PGKGVGEKAVFGGLLGEAAIIRVPGGDSTGFVKLGGRIPAPIHSLKN"
BASE COUNT          666 a         1234 c         1127 g          674 t
ORIGIN      
        1 gcagccccgc cacgctgtcg cgcagacgca ccttgagggc ggcgtcgccg aagatctcgc
       61 ctcgagtcag gtcgtcgtgc ccgacgcttg aggaggtgcc tcccgcgctc accgtctgcc
      121 tgacctcaag gtcgcccgcg cgcacgcgtc ccccgagcgt gacgtcggcc ttgatccagg
      181 acgaggacgc cggacggaag agcctgtgcc aacccgcctg cagctgcagc gtgcccaccc
      241 acctgctctc ctcctccacg tccatgacac gctcaccgtc aaaggcgatc ccatatgtcg
      301 acttaagata attcgtcagg gagacggcgc ccgtgaagtt gacgtccttg tagccgcccc
      361 agtcgggata gaagcttccc gcgacgcccg ccgtcatggc ccggcgggac ggatcgcccg
      421 tggtgagcat gtcgcccgtc gagatgacgc ggaccttgtc gttggcgccc gagaacatcc
      481 cgtagcccgt caccgcaaac ctgtcgcttg attcgccgta gtagactgcc agggaagcga
      541 tgttcgactc ctgactgacg tcgaagacat cgaaggagct tccgacgtcc gcgtcggcat
      601 aggagacgac cagcccgccg aatctggtga ggtgcctgag atgcacctcg aggtcaagcc
      661 ccgtcacgtc cctcgaccac tcggcgaatc ctcctgtact gccatcgacg ctcttgaagc
      721 gccccctgct cgagcctgtt ctcgcggaca ccctgacggc ctggtcgtcc tcgaaggcga
      781 ctgcagccct tgacgtgagc tcgacgacgt tctccgccgt cctgatcatc tccgtcgaag
      841 tgcccgcccc caccatcagt tcggtcatcc agttgatggt cttgccgaat tcactcgggc
      901 tctgcctcct gaggaactcc ttgcgcagcg ccttgggcgc atcggccttc atggcttcga
      961 cgcccagtgc gttcacgacc tcctcaagtg gcccctcgta gcgccactcc cgctcaacga
     1021 ccctccaacc gtcctccgtc tgctcgaact tcatcgagca cacctgccca tccttggtca
     1081 gaaaaccctc gaggtgctcc acgcccgtga cctccgatcc cgcgaccgcc ttaaagacga
     1141 ggcgctcttc cacaggcact tcctgcgcct cgtcgtccgc ctcaaactct tcaggttcgc
     1201 gcaggtcgtc catgccgtcc gtgatgatga agacggcccc cttctcaaag acggccttcg
     1261 tccccttgcc gaacgtgatc gcccccttgt cctcggcgag tcccatgaac cacgacttgt
     1321 tgatcacgac gcccgccgac ggtcccagat ggaagttggc attcgtctct tcggagccct
     1381 cgacgccgaa gctcatctgc acatctggcg tgacgtggag atgccctgca atgacaagca
     1441 cgtcctgagg gttcctgccc accgtttcaa gcgcggcgcg cccgccgttt gcgaacgcat
     1501 gcacaagctt cgcccccgcc ttgaactgac cgtccgaata atcagcctga ttctcgtcgg
     1561 aaagcagacg aaatcccccc agatcaccga ggtcttcgtc gggattttcg ttgccagaac
     1621 cgccgtcctg accatcatcc tcaggcaggt tcgacgtgtc catcacgatg gtcgttgtgc
     1681 cgcccgaagc cagcacgttc acgggaccct tgccggcggg aaggaccagc gagacgcccg
     1741 tgagggcgtt cgagacgccg aagccctgaa gcctgaaggt cgtttcctgc tcgacacgag
     1801 gggcgatcag gacgttgttg aagcccgccg tgatcgttgc ggtcttcacg cccgtcacaa
     1861 gaacgtcgcg cgtgtcctca cccggaacga agacggtatg ctcgaaggga tcgagctcga
     1921 tttccatgct ctcctgcgcc attgcggggg cgccaagggc agcggtgacg gcaccggaaa
     1981 ggagtgagag gcggacggcg ttgaggccca gagaagggcc gatgggggtg ctggctttca
     2041 tgagggagca tccttaaggt tgcgttgtcg ggcttcgttc cgctcgtacg gacatatagg
     2101 aaaaacaaac ggcaaacccc aaggcttcat tcccgaaacg attgtggcac acgcgtttcc
     2161 atcatacacg cagtatcaat accacttatc atatcggtac tcatgcgaaa ctcaccacat
     2221 agcgtatcgc actccctgca caatttccac caaaaccagt atttaccctc tcttctagca
     2281 aaaaaaaggc gccgcctcca aaacaggagg acggcgccgt gaatggtcgt cgcgacaggc
     2341 ggacggagcg tccgccgcga cccggacagg ttcagccgat cagttcttga gggagtggat
     2401 cggcgcgggg atgcggccgc cgagcttcac gaagcccgtc gagtcgccgc cgggcacgcg
     2461 gatgatcgca gcttcgccga gcaggccgcc gaagacggcc ttttcgccga cgcccttgcc
     2521 gggcacggga atgatgcgca cggcggtggt cttggagttg atcatgccga tggccatttc
     2581 gtcggcgatc atgccggaga tcgtggccgc ggacgtgtcg ccggggatgg cgatcatgtc
     2641 gagaccgacg gagcagacgc tcgtcatcgc ctcaagcttc tccatcgtga gggcgccgct
     2701 gcgggccgcg gcctcgatgg cgctgtcctc ggagacgggg atgaaggcgc ccgaaaggcc
     2761 gcccacatgc gaggaggcga agacgccgcc cttcttcacg gcgtcgttga gcatggccaa
     2821 aatggccgtc gtgccgggag cgccgatcga ggagaggccc atcgtctgga agatctcgcc
     2881 cacgctgtcg cccacggcgg gcgtcggcgc cagggagagg tcggccacgc cgaacggcag
     2941 gttcaggcgc tgggcgacct cgttgccgat gatctcgccc acgcgggtga ccttgtaggc
     3001 cgtgtgcttg atgacctcgg cggcgtccgt gatcgtgaga tagtcgccct tggcctcgaa
     3061 ggcgcggtca agcgccttct tgacgacgcc tggacccgag acgcccacgt tgatcacgac
     3121 gtcgggctcg cccacgccga ggtaggcgcc cgccatgaac ggcacgtcct gcggaatatt
     3181 gcagaagacg acgagcttgg cgcagccgat ggcgtcacgg tcggccgtgg ccgcggccac
     3241 gtcgaggatg cgctcgccca tgagcttcac ggcgtccatg ttgatgccgg ccttggtcga
     3301 gcccacgttg atcgaggaac agatgcggtc cgtggtggca agggcctcgg gaagcgcgtc
     3361 gatgaggttg cgttctccgg gcgtcatgcc cttttcaacg agcgcaccga agccgccgat
     3421 gaagtcgacg cccacgtcct tggcgcagtc gtcgagcacg cggcacgcct cgaccatctg
     3481 gtcgcgcgtg aagcccgcgc agacggtggc catcgggctc accgaaattc gcttgttgac
     3541 gacgggaatg ccgtagcggt cgccgacgat gtcgcaggtt tcaacgagct tctcggcgtt
     3601 cctgaggatc ttgctgcgga ccttgtaggc gaacgtgtcg aagtcggggg ccgcgcagtc
     3661 gaagagcgag atacccatcg tgacggttct gacatcgagg t
//
LOCUS       DBMDTI010000196         3693 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5745_length_3693_cov_2.413419, whole genome shotgun sequence.
ACCESSION   DBMDTI010000196 DBMDTI010000000
VERSION     DBMDTI010000196.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5745_length_3693_cov_2.413419"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="ACZPB6_08270"
     CDS             complement(<1..1452)
                     /locus_tag="ACZPB6_08270"
                     /EC_number="4.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016841 - ammonia-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0170035 - L-amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAL/PAL/TAL family ammonia-lyase"
                     /protein_id="HIR1184498.1"
                     /translation="MYKFFKMTALCAALAAAGAAGAATITLDGTSLSIEDAWKIAAGE
                     ADVAIAPEAVKLLEDSHKLVMASAAKGQAVYGLTVGVGLNKDQKLFTAEGKLSPEVLE
                     ASRAFNYNALRSHSAGVGEMMPVDLARLSMVVRLNTLLAGKSGAQVRVAELYRDMLNK
                     NVTPLIPSEGSVGEADILLASHVGAVMIGEWKADVKGKVMTGADALKAGGIKPLQSEG
                     KDALAILSNNSVAMAYAIDAARNAERIVEMTPTVYGLSLEGLNGNVAPILPQTIGARP
                     FDGLSQTAADMRDVLKGSYLWNVDATRPLQDPLSYRVTVYGLSEARRAVKDLNDQIAI
                     QINSTDDNPATILNADEAYRSESTQVANYFVEGNGVKGAIIPSGNFNPLPVALALQRT
                     SIAMAHLSHYSVQRTVHLSYDQFTGLTRFLTDPSNKGHAFGAIQKSFMGLHVDNMALA
                     QPVSLYGMPVAGEIEDTFTNLLQASKRLGSIDTN"
     gene            complement(1500..1874)
                     /locus_tag="ACZPB6_08275"
     CDS             complement(1500..1874)
                     /locus_tag="ACZPB6_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR1184499.1"
                     /translation="MLKTKLFAAAMLAAALGLSTTANAGPAELKPHHKALGDCALCHT
                     QENAVAGNAFVVPDNKACLACHGSWQDLAEKTKPTDPHEPNPHKSHHYGENMSCTACH
                     AEHKESRVICNDCHAFPFKPMK"
     gene            complement(1899..3416)
                     /locus_tag="ACZPB6_08280"
     CDS             complement(1899..3416)
                     /locus_tag="ACZPB6_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HIR1184500.1"
                     /translation="MTQNINRTRRSLLAGGTMAALAAATGAAHAKEGAPAFDKIYDVI
                     VVGSGFAGLAAALQARLKGAEVLLIEKMPVFGGNSAINGGAFAVAGSPLQEKEGIKDS
                     PERMLQDMIRSGRGLSHVDLLKMIVEGTRPAFDFTLEHGVQYKPFVQHFGGHSVPRIM
                     QTVESTGGGITRPLADSCRKHGVDMHLNAKMESFIRDADGRVIGLKVRERYQFPKEDS
                     GVLQTYGTRKGVIMATGGFSRNLWFRMQQDPSLDDRLDSTNHLGATGEGMLEMFAIGG
                     APVQVDQIQLGPWSSPDEKGFGLVSQFNTIAGFPMGIMVDVRTGKRFCNELADRKART
                     DAILGLMENGKPVYPVCFTDSKGVAKAQTLRNGLKYKVIWQFDTVEALADHFKIPAAE
                     LKAQVARWNEMVAAGNDKEFGRALQLAIQLDKPPFYACRVWPKVHYCMGGVGIDHQAR
                     VLTVHGKPIEGLYAAGEVTGGAHGASRLGGCAIADGLVFGRIAADTAVAAKPAAL"
BASE COUNT          568 a          988 c         1423 g          714 t
ORIGIN      
        1 gttcgtgtcg atgctgccga ggcgcttgga ggcctgaagg aggttcgtga acgtgtcttc
       61 gatttcaccg gcgacgggca tgccgtagag gctcacgggc tgggcgagcg ccatgttgtc
      121 gacgtgcagg cccatgaagg acttctggat ggcgccgaag gcgtggccct tgttgctcgg
      181 gtcggtgagg aagcgcgtga ggccggtgaa ctggtcgtag gagaggtgca ccgtgcgctg
      241 gaccgagtag tgggagaggt gggccatggc gatcgaggtg cgctggagcg caagggcgac
      301 gggcagcggg ttgaagttgc ccgacgggat gatggcgccc ttgacgccgt tgccctcgac
      361 gaagtagttg gcgacctgcg tggactcaga gcgataggct tcgtcggcgt tgaggatcgt
      421 ggcgggattg tcgtccgtgg agttgatctg gatggcgatc tggtcgttga ggtccttgac
      481 ggcgcggcgg gcttcgctca gaccgtagac ggtcacgcgg taggaaagcg ggtcctgaag
      541 cggacgcgtg gcgtcgacgt tccagaggta ggagcccttg aggacgtcgc gcatgtcggc
      601 ggcggtctgg gagaggccgt cgaagggacg ggcgccgatc gtctgcggga ggatcggggc
      661 gacgttgccg ttgaggcctt cgagcgagag gccgtagacg gtcggggtca tctcgacgat
      721 gcgctcggcg ttgcgggcgg cgtcgatggc gtaggccatg gcgacggagt tgttggagag
      781 gatggcgagc gcgtccttgc cttcggactg gagcggcttg atgccgccgg ccttgagggc
      841 gtcggcgccc gtcatgacct tgcccttcac gtcggccttc cattcgccga tcatgacggc
      901 gccgacatgg gaggcgagca ggatgtcggc ctcgccgacg gagccttcgg acgggatgag
      961 cggcgtcacg ttcttgttga gcatgtcgcg gtagagctcg gcgacgcgga cctgggcgcc
     1021 ggacttgccg gcgaggagcg tgttgagccg cacgaccatg gagagtcggg cgaggtcgac
     1081 gggcatcatt tcaccgacgc cggcggagtg cgagcgcagg gcgttgtagt tgaacgcgcg
     1141 ggaggcttcg agcacttcgg gggagagctt gccttcggcg gtgaagagct tctggtcctt
     1201 gttgaggccg acgcccacgg tgaggccgta gacggcctgg cccttggcgg cggaggccat
     1261 gacgagcttg tggctgtcct cgaggagctt gacggcttcg ggagcgatgg cgacgtcggc
     1321 ttcgcccgcg gcgatcttcc aggcgtcctc gatggagagg ctggtgccgt cgagcgtgat
     1381 cgtggcggcg ccggcggcgc ccgccgcggc gagagcggcg cagagtgcgg tcatcttgaa
     1441 gaacttgtac atggttgaaa ttcctttagt gtacggcgtg gttcggggcg cgacgggcct
     1501 tacttcatcg gcttgaacgg gaaggcgtgg cagtcgttgc agatcacgcg gctttccttg
     1561 tgctcggcgt ggcaggccgt gcaggacatg ttctcgccgt agtggtggga cttgtgcgga
     1621 ttgggctcat gcggatcggt gggcttcgtc ttttcggcga ggtcctgcca ggagccgtgg
     1681 caggcgaggc aggctttgtt gtcggggacg acgaaggcgt tgccggcgac ggcgttttcc
     1741 tgggtgtggc agagcgcgca gtcgccgagg gccttgtggt ggggcttgag ttcggcggga
     1801 ccggcattgg ccgtcgtgga gaggccgagc gcggcggcga gcatggccgc agcgaagagt
     1861 ttggtcttga gcattgggag tcctttggtt cgaaatcgtt agagagcggc gggcttggcg
     1921 gcgacggcgg tgtcggcagc gatgcggccg aagacgaggc cgtcggcaat ggcgcagccg
     1981 ccgagacggc tggcgccgtg ggcgccgccc gtcacttcgc cggcggcgta gaggccttcg
     2041 atcggcttgc cgtggacggt gaggacgcgg gcctggtggt cgatgccgac gccgcccatg
     2101 cagtagtgga ccttcggcca gacgcggcag gcgtagaagg gcggcttgtc aagctggatg
     2161 gcgagctgca gcgcgcggcc gaactccttg tcgttgccgg cggcgaccat ttcgttccaa
     2221 cgggcgactt gggccttgag ctcggcagcc gggatcttga agtggtcggc gagggcctcg
     2281 accgtgtcga actgccagat gaccttgtac ttgaggccgt tgcgcagcgt ctgggccttg
     2341 gcgacgccct tgctgtcggt gaagcagacc gggtagacgg gcttgccgtt ttccatcagg
     2401 ccgagaatgg cgtcggtgcg ggccttgcgg tcggcgagct cgttgcagaa gcgcttgccg
     2461 gtgcgcacgt cgaccatgat gcccatcggg aagccggcga tcgtgttgaa ctgggagacg
     2521 agaccgaagc ccttttcgtc gggagaggac caggggccga gctggatctg gtcgacttgg
     2581 accggagcgc cgccgatggc gaacatttcg agcatgcctt cgcccgtggc gccgaggtgg
     2641 ttcgtggagt caagacggtc gtcgagcgac ggatcctgct gcatgcggaa ccagaggttg
     2701 cgggagaagc cgcccgtggc catgatcacg cccttgcgtg tgccgtaggt ctggaggacg
     2761 ccggagtctt ccttcgggaa ctggtagcgt tcgcggacct tgaggccgat cacgcggccg
     2821 tcggcgtcgc ggatgaagga ttccatcttg gcgttgaggt gcatgtcgac gccgtgcttg
     2881 cggcagctgt cggcgagcgg acgggtgatg ccgccgcccg tggattcgac ggtctgcatg
     2941 atgcgcggga cgctgtggcc gccgaagtgc tgcacgaagg gcttgtactg gacgccgtgc
     3001 tcgagcgtga agtcaaaggc gggacgcgtg ccttcgacga tcatcttgag caggtcgacg
     3061 tgggagaggc cgcggccgga acggatcatg tcctgaagca tgcgttcggg actgtccttg
     3121 atgccttcct tttcctgaag cggagaaccc gcgacggcga aggcgccgcc gttgatggcg
     3181 gagttgccgc cgaagacggg catcttttcg atgagaagga cttcggcgcc cttgaggcgg
     3241 gcctgaagcg cggcggcaag gccggcaaag cccgagccca cgacgatcac gtcgtagatc
     3301 ttgtcgaagg cgggagcgcc ttccttggcg tgcgccgcgc ccgtggcggc ggcgagagcg
     3361 gccatcgtgc cgccggcgag aaggctgcgg cgggtgcggt tgatgttctg agtcatgagg
     3421 ttctccttgg aatgactggt ctggatgctc ttttaaaagc aagaggcatg ccaaactttg
     3481 gtgcgcgcgt gccgggcctt gtgccgcaag cgtttgcgca aaacctagga gaaacccgcc
     3541 cgaggtgtta acccttgaag atccgacctt tcatgcatgc ggagttacgg aatgtcgtaa
     3601 catgagaaac gacatttctc tcttttacga tattgcgtac tctttcaaga catgagcgaa
     3661 gcctattttc ccgacgatct gcgtctgagg acc
//
LOCUS       DBMDTI010000197         3686 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5760_length_3686_cov_2.215342, whole genome shotgun sequence.
ACCESSION   DBMDTI010000197 DBMDTI010000000
VERSION     DBMDTI010000197.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5760_length_3686_cov_2.215342"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            72..1127
                     /locus_tag="ACZPB6_08285"
     CDS             72..1127
                     /locus_tag="ACZPB6_08285"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II asparaginase"
                     /protein_id="HIR1184501.1"
                     /translation="MNFKKTLLAAALTLSAGFAMAADLPQVAILATGGTIAGTAASAT
                     QTTGYKAGDLAIQTLIDAVPQIKDYATVTGEQIVKISSNNLTDQVLLTLARRCNELLA
                     DPKVSGIVITHGTDTLEETAFFLNLTVKSKKPVVVIGAMRPATAISADGPMNLLNAVK
                     LAADPKAQDRGVLVALNDQISSGRDVTKSNTTNVATFKSPDLGYLGYIAGGKNYFLRN
                     PAMRHTHQSEFDVSKLDKLPRVDILYTHASDDRVLADAAIAAGAKGIVHAGSGNGSVH
                     GQTEPALAEAVQKGIAVVLSSRTGSGVVCPNVEQYNKAGFIEGRTLNPQKARLLLQLA
                     LTKTNDPKEIARMFEEY"
     gene            1413..>3686
                     /gene="purL"
                     /locus_tag="ACZPB6_08290"
                     /gene_synonym="purI"
     CDS             1413..>3686
                     /gene="purL"
                     /locus_tag="ACZPB6_08290"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR1184502.1"
                     /translation="MSTCNGMVFELNGARALSDFRAARVLAALQRVSSNIEAVSGRFV
                     HFVHASRELTKAEEERLASLLTYGDAAEDVRADLAFMVVPRLGTISPWASKATDIVKN
                     CGIEGVLRVERGTVYSLALKAPLTEEEAAQAAGVLHDRMTESVVARDFPAENLFVELE
                     GRPMATVALVEEGRPALERANVEMGLALSPDEIDYLTDAFTKIGRNPTDVELMMFAQA
                     NSEHCRHKIFNARWTVDGEEREETLFGMIRRTHKMAPQGTITAYADNAAIFEGAEVTR
                     LYPRPGSGNEFGRVFERKDEMTHTVFKVETHNHPTAISPFPGASTGSGGEIRDEGATG
                     RGARPKAGLCGFTTSNLNLPELPQGFENDSDTVTGEKTDAKYGAPSRIATPLSIMTEG
                     PLGGAAFNNEFGRPNILGYFRTFEANIGGTRYGYHKPIMLAGGIGNIRDDQTKKDVPP
                     AGSLLIVLGGPGMRIGLGGGAASSMTTGSNSEALDFDSVQRGNPEMERRAQEVIDRCW
                     SMGDENPIIAIHDVGAGGLSNAMPELADLSGKGATFDLSKVPVEESGMSPLEIWCNES
                     QERYVIALDAAKIDIFRDFCERERCPFAVLGTITEEADLKLTRPEETPAVDMPMEVLL
                     GKAPRMHRDVAHVETKLSAFKSEGLDLAKAVTDVLRHPTVGSKSFLITIGDRSVGGLV
                     SRDQMVGPWQVPVADCGVTTLGFETNRGEAMSMGERTPIAVIDAAAASRMAVGEALTN
                     IAAADVKLPEVKLSLNWM"
BASE COUNT          698 a         1332 c         1061 g          595 t
ORIGIN      
        1 cgctccgaca tgggcgcgga catgacccgg gaccgcagag cccggatgtt gacccaaacc
       61 aaaggacaga catgaacttc aagaagacgc ttctcgccgc cgcgctcacg ctttccgcag
      121 gctttgccat ggccgccgac cttccgcagg tcgcgatcct cgccacgggc ggcacgattg
      181 ccggcaccgc cgcctccgcc acgcagacca cgggctacaa ggcgggcgac ctggccatcc
      241 agacgctgat tgacgctgtt ccgcagatca aggactacgc gacggtgaca ggcgagcaga
      301 tcgtgaagat ctcgtccaac aacctgaccg accaggtcct cctgacgcta gccaggcgct
      361 gcaacgagct tctcgccgat ccgaaggtga gcggcatcgt catcacgcac ggcacggaca
      421 cgcttgaaga gaccgctttc tttctcaacc tcacggtcaa gagcaagaag cccgtggtgg
      481 tgatcggcgc catgcgtccc gccacggcca tttcggccga cggtccgatg aaccttctca
      541 acgccgtcaa gctcgccgcc gatccgaagg cgcaggaccg tggcgtgctc gtggccctca
      601 acgaccagat ctcatccggc cgcgacgtca cgaagtccaa tacgacgaac gtcgccacat
      661 tcaagtcgcc cgacctgggc tacctcggct acatcgccgg cggcaagaac tacttcctgc
      721 gcaaccccgc catgcgtcac acgcaccaga gcgaattcga tgtctcgaag cttgacaagc
      781 tgccgcgcgt cgacatcctc tacacgcacg ccagcgacga ccgcgtcctt gccgacgccg
      841 ccatcgccgc gggtgccaag ggcatcgtgc acgccggttc cggcaatggt tccgttcacg
      901 gccagaccga gcccgcgctt gccgaagccg tccagaaggg catcgccgtg gtgctctcct
      961 cgcgcacggg ctccggcgtg gtctgcccga acgtggagca gtacaacaag gccggcttca
     1021 ttgaaggccg cacgctcaat ccccagaagg cccgtctcct tctccagctc gctctgacga
     1081 agacgaacga tccgaaggaa atcgcccgca tgttcgagga atactgatcc tttcccgaac
     1141 ccgccggcat gacgccgccc gcagggtgaa aacctcctga aggcggcttc ccgagcattc
     1201 ggccgtcctg ctcccatgcg gagtcggggc ggtctttttc tttgatgcgg aaaaaaaacg
     1261 aaggtttgca tgggaatggc cagaatcggc gccggagcag ggtttgagga aggcaaaacc
     1321 ctctataatg gcgacatcct tttttctttt attttccacc ccgtcacggc cgtcggaaaa
     1381 tccggggcct gctgacgatt cagaggaaac ttatgtcgac ctgcaacggt atggtttttg
     1441 aactcaacgg cgcccgcgcg ctctcggact tccgcgccgc gcgcgtgctc gcggccctgc
     1501 agcgcgtctc ttccaacatc gaagcggtct cgggccgctt cgtgcatttc gtgcatgcct
     1561 cccgcgagct cacgaaggcc gaagaggaac gcctcgcttc gcttctcacc tacggcgacg
     1621 ccgccgagga cgtccgcgcc gacctggcct tcatggtggt gccgcgtctt ggcacgatct
     1681 cgccctgggc ctcgaaggcc acggacatcg tgaagaactg cggcatcgaa ggcgtcctgc
     1741 gcgtcgagcg cggcacggtc tacagccttg ccctcaaggc tcccctcacg gaagaggagg
     1801 ccgctcaggc cgccggcgtg cttcatgacc gcatgaccga atccgtcgtc gcccgcgact
     1861 tcccggccga aaacctcttc gtcgagctcg aaggccgtcc gatggcgacc gtcgccctcg
     1921 tcgaggaagg tcgtcccgcc cttgagcgcg ccaacgtcga gatgggcctt gcgctctctc
     1981 ccgacgagat cgactacctc accgacgcct tcacgaagat cggccgcaac ccgaccgacg
     2041 tcgagctgat gatgttcgcg caggccaact ccgagcactg ccgtcacaag atcttcaacg
     2101 cccgctggac ggtcgacggt gaagagcgcg aggaaacgct cttcggcatg atccgccgca
     2161 cgcacaagat ggccccgcag ggtacgatca cggcctacgc cgacaacgcc gccatcttcg
     2221 aaggcgccga agtcacgcgc ctctatccgc gtccgggctc cggcaatgaa ttcggccgcg
     2281 tcttcgagcg caaggacgaa atgacccaca cggtcttcaa ggtcgagacc cacaaccacc
     2341 cgactgccat ttctccgttc cccggcgcct ccacgggctc cggcggcgaa atccgcgacg
     2401 aaggtgcgac gggccgcggt gctcgtccga aggccggcct gtgcggcttc acgacctcga
     2461 acctcaacct gccggaactt ccgcagggtt ttgaaaacga ctccgacacg gtgacgggtg
     2521 aaaagacgga tgccaagtac ggcgccccgt cccgcatcgc cacgccgctc tcgatcatga
     2581 cggaaggccc gctcggcggc gccgccttca acaacgagtt cggccgtccg aacatcctcg
     2641 gctacttccg cacgttcgaa gccaacatcg gcggcacgcg ctacggctac cacaagccga
     2701 tcatgctcgc cggcggcatc ggcaacatcc gcgacgacca gacgaagaag gacgtgccgc
     2761 ccgccggcag cctcctgatc gtcctcggcg gccccggcat gcgcatcggc ctcggcggcg
     2821 gcgccgcctc gtccatgacg acgggctcca actccgaagc cctcgacttc gactccgtgc
     2881 agcgcggcaa ccccgaaatg gaacgccgtg cccaggaagt catcgaccgc tgctggtcca
     2941 tgggcgacga aaatccgatc atcgccattc acgacgtcgg cgcgggcggc ctctccaacg
     3001 ccatgcccga gcttgccgac ctttccggca agggcgccac gttcgacctc tccaaggttc
     3061 cggtcgaaga gtccggcatg agcccgctcg aaatctggtg caacgaatcc caggaacgct
     3121 acgtcatcgc gcttgacgcc gccaagatcg acatcttccg cgacttctgc gaacgcgagc
     3181 gctgcccgtt cgccgttctc ggcacgatca ccgaggaggc cgacctcaag ctcacgcgtc
     3241 ccgaagagac cccggccgtc gacatgccga tggaagtcct cctcggcaag gccccgcgca
     3301 tgcaccgcga cgtcgcccac gtcgaaacga agctctccgc cttcaagagc gaagggctcg
     3361 atcttgccaa ggctgtcacg gacgtcctgc gtcacccgac cgtcggttcc aagtccttcc
     3421 tcatcaccat cggcgaccgc tccgtgggcg gcctcgtctc ccgcgaccag atggtcggcc
     3481 cctggcaggt gcccgtcgcc gactgcggcg tgaccacgct cggctttgaa acgaaccgcg
     3541 gcgaagccat gtcgatgggc gaacgcaccc cgatcgccgt gatcgatgcc gctgctgcga
     3601 gccgcatggc cgtgggcgaa gcgctcacca acatcgccgc cgccgacgtg aagctccccg
     3661 aagtcaagct ctccctcaac tggatg
//
LOCUS       DBMDTI010000198         3676 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5778_length_3676_cov_2.645035, whole genome shotgun sequence.
ACCESSION   DBMDTI010000198 DBMDTI010000000
VERSION     DBMDTI010000198.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5778_length_3676_cov_2.645035"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="ACZPB6_08295"
     CDS             <1..640
                     /locus_tag="ACZPB6_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013045.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR1184503.1"
                     /translation="WPVHRLDRGTSGVLVFACDADTASRLGRIMMAGEVKKRYAAVVR
                     GWLEESVDCRHPLSLPEDPYLKKRRGPSEPQDARTVFIERARGEAPVPSGRFETTRLG
                     LVEAELFTGRRHQIRRHLKWLAHPVIGDATYGKGPLNRAVAQWFGADRLMLHCARMTL
                     PHPVTGEEIDIRAPLVEGPFRSAVERLGWLEAYEELMNGPWREPPAQAQLLA"
     gene            complement(722..1570)
                     /gene="ppsR"
                     /locus_tag="ACZPB6_08300"
     CDS             complement(722..1570)
                     /gene="ppsR"
                     /locus_tag="ACZPB6_08300"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HIR1184504.1"
                     /translation="MTDTQKCAPCRQIFIVSDGTAITAETLAHSIMTQFPDLDYHQTR
                     IPFVNTVEKALETAQRINQVEKEEGLRPIIFTTFVDPDIMRTFNEHVSGHIIDLFRKF
                     VGPLETELGMKSAHSIGQSHRIRDDAKYNRRIAAIDYTLQHDDGQTNRGLDEADVILV
                     GVSRSGKTPTSLYLAMQFGIKVANYPLIPEDFDRGSLPDALIPLREKLFGLSIKPERL
                     SEIRNERRPNSRYASIENCRQEIADAEDLMAREGIRWLNSTTKSIEEISATIMADLGL
                     DKRRNS"
     gene            complement(1673..2362)
                     /locus_tag="ACZPB6_08305"
     CDS             complement(1673..2362)
                     /locus_tag="ACZPB6_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR1184505.1"
                     /translation="MIDLILNEPQLLFSLALTAKTCLVTLALFLVAGPLAGYYLARAP
                     GVAPRVLGFIITIPLVFPPVALGYMLLTALGRTSLVGGALEPFGLSLIFNETAVALAG
                     FIAGLPLVVRPLQAAFASPRLIELEEAARIHGAGRRDVFLRITLPLVRPSLAAALLLG
                     CARVSGEVGITMMLGGNISERTNTLSLEIFNAVSRADFDAANALCLLLSLFAAVIFLA
                     AELLIRRSPRL"
     gene            complement(2387..3121)
                     /gene="modA"
                     /locus_tag="ACZPB6_08310"
     CDS             complement(2387..3121)
                     /gene="modA"
                     /locus_tag="ACZPB6_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1184506.1"
                     /translation="MKLKPLLISLAAVSALAAGAANAAQVIVTTGAGYVKMVDALAAL
                     YQKETGVAVEKSFGGNIGQMLAQVKHGSGVNVVISDEASLEKFHDALAPDARRLGDTP
                     LVLVWRKGLELKRPEDIALDSVKTVASPNPKAAVYGRSAAKWLKATGLDAKVAPKLQV
                     VGTVPQVYSYVTTGNMDAGFVNLAVLKSGTEALGGHAAIRDESAVVHMTVRPVRGAEA
                     DADVKAFMDFLASEKAKPVLRKFGIE"
     gene            complement(3153..>3676)
                     /locus_tag="ACZPB6_08315"
     CDS             complement(3153..>3676)
                     /locus_tag="ACZPB6_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1184507.1"
                     /translation="LTLIVPASGTISVNGRVFRSDDPCIDLPVESRGIGFVFQSHRLF
                     PHLTVRENLLFGRRFGGRRTAVDQDRLIEVLGIAHLLSRRPDTLSGGESQRVALGRAI
                     LAAETLLIMDEPLASLDSERKAELLGYFERIPEITGIPVLYITHAADEAERLAKSVLT
                     MKDGRIADIRTPA"
BASE COUNT          605 a         1060 c         1360 g          651 t
ORIGIN      
        1 ctggcccgtg cacaggctcg accgcgggac gtcgggcgtg ctcgtctttg cgtgtgatgc
       61 ggacacggcc tcgaggctcg ggcgcatcat gatggcgggc gaggtgaaga agcgctatgc
      121 ggccgtcgtg cgcggctggc tcgaggaatc cgtcgactgc cgccatccgc tctcgctccc
      181 cgaggatccg tatctgaaga aacgacgcgg tccgtcggag cctcaggatg cgcgcacggt
      241 cttcatcgag cgcgcaaggg gcgaggcgcc cgttccttcg ggcaggttcg agacgacccg
      301 cctcggcctc gtcgaggccg agctcttcac gggcagaagg catcagatcc gccgtcatct
      361 caagtggctt gcgcatcccg tcatcgggga cgccacctac gggaagggcc ccctcaaccg
      421 tgcggtcgcg caatggttcg gtgcggaccg cctgatgctc cactgtgcga ggatgacgct
      481 cccgcatccc gtcacgggcg aggagatcga catccgtgcg ccgctcgtcg agggtccctt
      541 caggtcagcc gtcgagaggc tcggctggct tgaggcgtac gaggagctta tgaacggccc
      601 ctggagagag ccgcctgcgc aggcgcagct tctggcgtag ctgcctgagg cccgggaaag
      661 aaagaaggcc cggccttccg agaagaagcc gggccttctt ggggtttgac tgtcgtcggc
      721 gtcaggagtt gcggcgcttg tcgaggccga gatcggccat gatcgtggcg gagatttcct
      781 cgatcgactt cgtcgtggag ttgagccagc ggatgccttc gcgggccatc aggtcttcgg
      841 cgtcggcgat ttcctgacgg cagttctcga tgctggcgta gcggctgttc ggacggcgct
      901 cgttgcgaat ttcggaaagg cgctcgggct tgatggagag gccgaagagc ttttcgcgaa
      961 gcggaatgag ggcgtcgggc agggagccgc ggtcgaagtc ctcggggatg agcgggtagt
     1021 tggcgacctt gatgccgaac tgcatggcga ggtagagcga cgtcggggtc ttgcccgaac
     1081 gggagacgcc cacgaggatc acgtccgctt cgtcgaggcc gcggttcgtc tggccgtcgt
     1141 cgtgctggag cgtgtagtcg atggcggcga tgcggcggtt gtacttcgcg tcgtcgcgga
     1201 tgcggtggct ctggccgatg gagtgcgcgc tcttcatgcc gagctcggtc tcgagcgggc
     1261 cgacgaactt gcggaagagg tcgatgatgt gaccgctcac gtgttcgttg aaggtgcgca
     1321 tgatgtccgg atccacgaag gtcgtgaaga tgatcggacg aaggccttct tccttttcca
     1381 cctgattgat gcgctgcgcg gtttcaagcg ccttctcgac cgtgttgacg aacgggatgc
     1441 gcgtctggtg gtagtcgagg tcggggaact gcgtcatgat cgaatgcgcg agcgtttcgg
     1501 cggtgatggc ggtgccgtcg ctcacgatga agatctgtcg gcagggggcg cacttctggg
     1561 tgtctgtcat ggcggataga tgaaaaggat tgggtaatcc ttccattcta accgatcggc
     1621 catttccgat gagtccagtc tgagggttcc cgcgtcgtac gggcgtttga ggtcagagcc
     1681 ttgggctgcg cctgatgagg agctcggccg caaggaagat gacggccgca aagagcgaga
     1741 gcaggaggca gagcgcgttc gcggcgtcga agtcggcgcg cgagacggcg ttgaagattt
     1801 cgagcgagag tgtgttggtg cgctccgaga tgttgccgcc gagcatcatc gtgatgccca
     1861 cctcgcccga gacgcgggcg cagccgagaa ggagcgccgc cgcgagactc ggccgcacga
     1921 gcgggagcgt gatcctgagg aagacgtcgc gcctgcccgc gccgtggatg cgggcggcct
     1981 cctcgagttc gatgaggcgc ggcgaggcga acgccgcctg aagcggacgc acgacgagcg
     2041 gtaggcccgc aatgaagccc gcgagcgcga cggccgtctc gttgaagatg agcgagaggc
     2101 cgaagggttc gagcgctccg cccacgaggc tcgtgcgtcc gagcgccgtc aagagcatgt
     2161 agccgagcgc cacgggcgga aagacgagcg gaatcgtgat aatgaagccg aggacgcgcg
     2221 gggctactcc cggcgccctc gcgaggtagt agcctgcgag cggccccgcg acgagaaaga
     2281 gcgcgagcgt gacgaggcag gtcttcgcgg tgagtgcaag cgagaagagg agctgcggct
     2341 cgttgagaat caggtcaatc atggcgggcg tgcgtcgcgg gcgtcctcat tcgatgccga
     2401 acttcctgag gacgggctta gccttttcgg aggcgaggaa gtccatgaag gccttcacgt
     2461 cggcgtcggc ctcggcgccc cgcacgggac gcacggtcat gtgcacgacg gcgctttcgt
     2521 cgcggatggc cgcgtggccg ccgagtgcct ccgtgccgga cttgagcacg gcgaggttca
     2581 cgaagcctgc gtccatgttg cccgtcgtta cgtaggagta gacctgcggc acggtgccca
     2641 cgacctgaag cttgggcgcg accttcgcgt caaggcccgt cgccttgagc catttggcgg
     2701 cggagcggcc gtagacggcg gccttcggat tgggcgaggc gacggtcttg acggagtcga
     2761 gcgcgatgtc ctcggggcgc ttcagctcaa gtcccttgcg ccagacgagg acgagcggcg
     2821 tgtcgccgag gcgcctggcg tcgggtgcga gcgcatcgtg gaacttctcg aggctcgcct
     2881 cgtcggagat gacgacgttg acgccgctgc cgtgcttcac ttgggcgagc atctggccga
     2941 tgttgccgcc gaagctcttc tcgacggcga cgcccgtctc cttctgatag agcgcggcga
     3001 gcgcgtcgac catcttgacg taacccgcgc ccgtcgtgac gatgacctgc gcggcgttcg
     3061 cggcgcccgc ggcaagagcg gagacggcgg caagggagat gagaagcggc ttgagtttca
     3121 tgatgagatc cttgggttgg gagtggtcgg tgtcaggccg gcgtgcggat gtccgcgatg
     3181 cggccgtcct tcatcgtgag gacgctcttc gcgagcctct cggcctcgtc ggcggcgtgc
     3241 gtgatgtaga ggacgggaat gcccgtgatt tcgggaatgc gctcgaagta gccgagaagc
     3301 tcggccttgc gctccgagtc gagactcgcg agcggctcgt ccatgatgag gagcgtctcg
     3361 gcggcgagaa tggcgcgccc gagcgcgacg cgctggcttt cgccgcccga gagcgtgtcg
     3421 ggacggcgtg aaagaaggtg cgcaatgccg agcacctcga tgaggcgatc ctggtcgact
     3481 gcagtgcggc gtcctccgaa gcgcctgccg aagagcaggt tctcgcgaac ggtgaggtgc
     3541 ggaaagagcc tgtgggactg gaagacgaag ccgatgccgc gcgattcaac gggaaggtcg
     3601 atgcacggat cgtccgacct gaagacgcgg ccgttgacgc tgatcgtgcc cgatgcgggc
     3661 acgatcagcg tcaacg
//
LOCUS       DBMDTI010000199         3675 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5781_length_3675_cov_2.807778, whole genome shotgun sequence.
ACCESSION   DBMDTI010000199 DBMDTI010000000
VERSION     DBMDTI010000199.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5781_length_3675_cov_2.807778"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(201..1427)
                     /locus_tag="ACZPB6_08320"
     CDS             complement(201..1427)
                     /locus_tag="ACZPB6_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HIR1184508.1"
                     /translation="MNVFLLIAAAIILIVVLLRFKCPIGPAILAGGALLWLFEDPTLR
                     ALTEAGHDMLTMNRTYDLLFALYFVMCLEIQLRRSGTLKGMVDALNRFFSSTKVVISS
                     MPAFLGLLPSVGGARFSAPIVETACEGMDIKPESKAAINFWFRHICEFASPIIPGMIL
                     GCAIAHVSVADLVIHLFWMSVVAFAAGWFVMVRPLKFEEKRRKAELSSEQRRTDLINV
                     VLAIAPVLLSVVLMMAFNLSAALAMGAVVVLLIPVLLMMKRGVSVKDVFVGAADWRLL
                     LNVGCILFFIQLLEATGILHQIITCFEGSALPVPVIIALTSFVIGILTGMSQGHVAIV
                     MPIVAGLMPGSLDLAGVAMVFGVAGQMITPTHVCLTITIDYFKSDFFKTLVPVILAEA
                     IVLVVFSAWTWLTWTA"
     gene            1731..>3675
                     /locus_tag="ACZPB6_08325"
     CDS             1731..>3675
                     /locus_tag="ACZPB6_08325"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR1184509.1"
                     /translation="MTESFNWLGRCARAAAFGIAALLLLTIARIVFFFAYKNDDLGFL
                     SDFAPAMVMGLRVDAKWLAILLLPAWICVLLSYWKAFFWKLARILAFVGMFVTALLTV
                     INFGFYGFYATPISPIIFGFLQDDTKAIVITIMKDWPVFTYLLCLAGFTALPFACTML
                     LGRSRERRVGRAAYGVLSILSIVVMALVIRGSLGTFPLRIQSYSVTKNAFINATVPNG
                     MAAFHEARKGMQVLELKGGAIAALQQMGFGKASDAEALIAEVRPVLPQSKPLKSQPHV
                     VLAVMESMGRDVFESHKPGVNDTLGALAGELDSALVFKQSLCVGQATFPTLEGLLFDS
                     PLNPITQSRYGRHVFDFSRMLEYKKAGYRTIFLTAGPEAWRQIETNFPQQGFDEIVGA
                     GKLSSRYPGVENGTWGVGDAWMFKRAEEILKEADQKGEKLFIVMLSTANHPPHRVPDG
                     VKVNPVSYKELPPFITDNRYDILTGSLETYQYAANALGGFVHDMRRGALKRELLIVAT
                     GDHNLRMSYAGGYAHHLHGVPILIWAPESLEADAGKVDVTRWAGHRDIFPTIAGVVLG
                     RTPEIHEGRNLFANESMDLIVSYTGIAAGSWGAAEIRDNGSVTCYRWENDRMVPEDAC
                     TGLSKKMADAARAQRALADYKVRK"
BASE COUNT          747 a         1016 c         1190 g          722 t
ORIGIN      
        1 cgcctgagcg ctgcggcttc gggcgcggag gagcggacgc gcgcggggtt ggacgcgcgg
       61 cttgaaagct gaacaaagtc aatattttac cccgtttgct gatactgacg ttaagcggaa
      121 gtcaaatcag gaagtgcggg gaaagaaagg ggcccgacgc gtgtgagcgt caggccccgc
      181 ctgatggatc agggatgcgg tcaggcagtc caggtgagcc aggtccaggc cgagaagacg
      241 acgagaacga tggcttccgc aaggatgacg ggcaccagcg tcttgaagaa gtccgacttg
      301 aagtagtcga tcgtgatcgt gaggcagacg tgggtgggcg tgatcatctg gcctgcgaca
      361 ccgaagacca tggcgacgcc ggccagatca agagagccgg gcatgaggcc cgccacgatc
      421 ggcatcacga tggcgacgtg gccctggctc atgccggtga ggatgccgat gacgaagctc
      481 gtgagcgcga tgatgacggg cacgggaagc gccgagcctt cgaagcaggt gatgatctgg
      541 tgcaggatgc ccgtggcctc aagaagctgg atgaagaaga ggatgcagcc gacgttgagg
      601 agaaggcgcc agtcggcggc gccgacgaac acgtccttga cgctcacgcc gcgcttcatc
      661 atcagaagta cggggatgag cagcacgacc acggcgccca tggcgagggc ggcggagaga
      721 ttgaaggcca tcatgagaac gacgctcaga agcacgggcg cgatggcgag cacgacgttg
      781 atgaggtctg tcctgcgctg ctcggacgac agttcggcct tgcggcgctt ttcctcaaac
      841 ttgagcgggc ggaccatgac gaaccagcct gcggcgaagg cgacgacgga catccagaaa
      901 agatggatga cgagatcggc gacgctcacg tgagcgatgg cgcagccgag gatcatgccg
      961 ggaatgatgg ggctggcgaa ctcgcagatg tggcggaacc agaagttgat ggcggccttg
     1021 ctttcgggct tgatgtccat accttcgcag gccgtctcga cgatcggagc ggaaaagcgt
     1081 gcgccgccga cagagggcag aagaccaagg aaggcgggca tgctggagat gacgactttc
     1141 gtcgaggaga agaagcggtt gagcgcgtcg accatgccct tgagcgtgcc ggatctgcgg
     1201 agctggattt caaggcacat cacgaagtag agcgcaaaga ggaggtcgta ggtgcggttc
     1261 atcgttagca tgtcgtgacc ggcttcggtg agggcgcgca gagtggggtc ttcaaagagc
     1321 cagagaaggg cgccgccggc cagaatggcc ggtccgatcg gacacttgaa gcgaagcagg
     1381 acgacgatga ggatgattgc cgccgcaatg aggaggaaaa cgttcattgt tgttgccttg
     1441 gtgatgggcg gtccctcctg aattcgggga ccgcgaatgt gcatcccgaa attttccgcc
     1501 tattctccga atttggcaaa aaatactttc cgaactcgcc atggccggag cgtgacgtgc
     1561 tcggagcgca acagattgag gaggtttcgt gcagaaaggc gtcgttcttg gggaaaaatg
     1621 atcgccctgt gaactgaaaa aaagttcgcg cctaagtaga atgcaccctt tggcctgcaa
     1681 cggcacggtg ccatgcgggt cctgcaatga ttcaacggct ctttcggaca atgacagaat
     1741 cctttaactg gctcgggcgg tgcgcccggg cagcggcttt cggcatcgcc gcactccttc
     1801 tgctgacgat tgcgagaatc gtcttcttct tcgcctataa aaacgacgat ctcggctttc
     1861 tgagcgactt tgcacctgca atggtgatgg ggcttcgcgt tgatgccaag tggctggcaa
     1921 tcctgctact gcccgcctgg atctgcgttc tcctttccta ctggaaggcc ttcttctgga
     1981 agctggcgcg cattcttgcc ttcgtgggca tgtttgtgac ggcgcttctg acggtcatca
     2041 acttcggctt ttacggcttc tacgcaacgc ccatcagccc tatcatcttc ggcttccttc
     2101 aggacgacac gaaggcgatc gtcatcacga tcatgaagga ctggcccgtg ttcacctacc
     2161 tgctttgtct ggcgggtttc acggctctgc cctttgcgtg caccatgctt ctcggtcgca
     2221 gccgtgagcg cagggtcggc agggccgcct acggcgtcct ttcgattctt tcgattgtcg
     2281 tgatggcgct tgtcattcgc ggtagcttgg gcaccttccc gcttcgaatc cagagctatt
     2341 ccgttacgaa gaacgccttc atcaacgcca ctgttcccaa cggcatggct gccttccatg
     2401 aggcccgaaa gggcatgcag gtgcttgaac tcaagggcgg cgccattgcc gccctccagc
     2461 agatgggctt tggcaaggca tcagatgctg aggctctcat tgccgaggtt cgccctgtac
     2521 tgcctcagag caagccgctc aagtcgcagc ctcatgttgt tctggccgtc atggagtcca
     2581 tggggcgtga cgtgttcgag tcgcacaagc ccggtgtcaa cgacacgctg ggtgctctgg
     2641 caggcgaact cgattcggcg cttgtcttca agcagtcgct gtgtgtggga caggcaacct
     2701 tcccgacgct cgagggcctg cttttcgact ctcccctgaa tccgatcacg cagagccgct
     2761 acggccggca cgtcttcgat ttctcccgta tgcttgagta caagaaggcg ggctacagga
     2821 cgatcttctt gacggcgggt cccgaggcct ggcgccagat cgaaaccaat tttccgcaac
     2881 agggcttcga cgagattgtc ggtgcgggca agctgtcgtc ccgctatccc ggcgtcgaga
     2941 acggcacgtg gggtgtcggt gacgcctgga tgttcaagcg tgcggaggag attctgaagg
     3001 aggcggacca gaagggggag aagctcttta tcgtgatgct ctccaccgcc aatcatccgc
     3061 cgcacagggt gcccgacggc gtcaaggtca atcccgtttc ctacaaggag ttgccgccct
     3121 tcatcacgga caacaggtac gacatcctga cgggttctct tgaaacctat cagtacgcgg
     3181 ccaatgctct gggcggattc gtgcatgaca tgagacgcgg cgcgctcaag cgcgagcttc
     3241 tgatcgtggc gaccggcgac cacaatcttc gcatgtcgta cgcaggcggc tacgcccatc
     3301 atttgcatgg cgttccgatt ctgatctggg cgccagagag ccttgaggct gacgccggga
     3361 aggtcgacgt cacgcgttgg gcggggcatc gcgacatctt cccgacgatt gcaggcgtcg
     3421 tgttgggcag gacgcccgaa attcacgaag gccgcaacct gtttgccaac gaatcgatgg
     3481 acctcatcgt ttcctatacg ggcatcgcag cgggcagctg gggtgccgcc gagatcaggg
     3541 acaacggaag cgtgacctgc tatcgctggg agaatgaccg catggtgcct gaggatgcct
     3601 gcacggggct ttccaaaaag atggccgatg cggccagagc ccagagggcg ctcgccgact
     3661 acaaggtcag gaagg
//
LOCUS       DBMDTI010000200         3624 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5872_length_3624_cov_3.120068, whole genome shotgun sequence.
ACCESSION   DBMDTI010000200 DBMDTI010000000
VERSION     DBMDTI010000200.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5872_length_3624_cov_3.120068"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="ACZPB6_08330"
     CDS             <1..305
                     /locus_tag="ACZPB6_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184510.1"
                     /translation="LWTLRFTGRRALPLVGPALVEELRKLPDYAEDELPEEIDSANPR
                     AVMVYVCRTFLHGIYDMVRTLTKADEMTPEKLSETVNSTAALWTLRFTGRRADKLN"
     gene            323..820
                     /locus_tag="ACZPB6_08335"
     CDS             323..820
                     /locus_tag="ACZPB6_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184511.1"
                     /translation="MVGCGSNCSAGCCKDFSSRAERLPNLLSVEVTGEIFAKAVRNMT
                     LRAAQRAAGMMSDEEYEEAKSKLVRWLVNAFSGENPHYEADSEWHGNGGLADYLAETL
                     DYHGHSPRQVITHAAALFADDADNLAREIVERGGLDTPAARQALAQVAGMWAQVFTGV
                     PPEFA"
     gene            829..1107
                     /locus_tag="ACZPB6_08340"
     CDS             829..1107
                     /locus_tag="ACZPB6_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184512.1"
                     /translation="MARSPLEEHAPDVTREIMGRLSPEAMRTLRAVSEMRNRPAEDVL
                     REELRGYIADKLPLPDVEAIIHAMGERFYALGYACGTAKRFLRKLRGE"
     gene            complement(1177..1773)
                     /locus_tag="ACZPB6_08345"
     CDS             complement(1177..1773)
                     /locus_tag="ACZPB6_08345"
                     /inference="COORDINATES: protein motif:HMM:NF027363.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HIR1184513.1"
                     /translation="MTHILKRLAAAACMTLTLGIAFAGQPLKIESITYEPQANVNGEQ
                     LLLNGAGLRTKIFFKVYTAGLYLKAPARRNTEVMAQNSAARVRLGLLRDVSCASFIDS
                     LNDGIKANLQPAKEKAISAGLEALRSLMRSIGDVKTGDIIDFDYSPDKGTTVHLNDKP
                     VGEPIGGGRALYNAVLAIWLGDNAIDDGLKKKLLKSGD"
     gene            complement(1858..2793)
                     /locus_tag="ACZPB6_08350"
     CDS             complement(1858..2793)
                     /locus_tag="ACZPB6_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1184514.1"
                     /translation="MKKAFLQLHISILLAGWTGVFGKLMMLSPGLIVFWRIVIAGGCL
                     WLWAWLTKRLEKVTPRDRIAIMGVGALLAVQWTLFYASIKASNVSIGVVAFSSIGFFT
                     ALIEPLMNRARISLREMLFSLVTIFGISLIFHFDAQYRLGIALGLVGAAAAALLAVYF
                     KRYRAKYSSVTVMSWQLLGGFAFSCVAIPIYVSLMPPEPFIPTWPDTLYLPIFAIFCT
                     LFMYILQIQSLEHISAFTVNLSYNLEPIYSIILAMVIFGEARDLGPSFYAGIGLIALS
                     VALQTSSVVRGRKRQKRIEARKAAESAKAGKTENS"
     gene            complement(3243..>3624)
                     /locus_tag="ACZPB6_08355"
     CDS             complement(3243..>3624)
                     /locus_tag="ACZPB6_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184515.1"
                     /translation="PLYGGGRNPPPSEPPELALGDSFSGLNDYDTGVKPGCYSSWNGL
                     TDPFRGVGLFLKIGVQQIQTSRRIAVDPTVVVIFTQSYAALPKFVLDNLTRQQIVLRG
                     LDLLWRYDFYQQTGAFSLACELSV"
BASE COUNT          725 a         1064 c         1176 g          659 t
ORIGIN      
        1 cgctctggac gctgcgcttc accggccgca gggcccttcc gctcgtcggt cccgcgctcg
       61 tcgaggagct gcgcaagctt cccgactatg ccgaggacga gctccccgag gagatcgaca
      121 gtgccaatcc gcgcgccgtc atggtctacg tgtgccgcac cttcctgcac ggcatttacg
      181 acatggtccg cacgctgacg aaggccgatg agatgacgcc cgagaagctc tccgagaccg
      241 tgaattcgac cgccgcgctc tggacgctgc gcttcaccgg ccgcagggcc gacaaactca
      301 actgaacgat cggagacggg acatggtcgg ttgcggatcg aactgcagcg caggatgctg
      361 caaggacttc agttccaggg cggagcgtct gcccaatctt ctgagcgtcg aggtgacggg
      421 cgagatcttc gcgaaggccg tccgcaacat gacgcttcgc gccgctcagc gcgccgcggg
      481 catgatgagc gacgaggagt atgaggaggc caagagcaag ctcgtgcgct ggctcgtcaa
      541 cgccttttcg ggcgagaatc ctcactatga ggccgactcc gagtggcacg gcaacggcgg
      601 tctcgccgac tatctggccg agacgctcga ctatcacggc cattcgcccc gtcaggtgat
      661 cacgcatgcg gccgcgctct ttgccgacga cgccgacaac ctcgcgcgcg agatcgtcga
      721 gcgcggcggc ctcgacacgc ccgcggcccg tcaggcgctg gcgcaggtcg caggcatgtg
      781 ggcgcaggtc ttcacgggcg ttccgcccga attcgcctga ggttagccat ggcgcgcagt
      841 ccgcttgaag agcacgctcc cgacgtcacc cgcgagatca tggggcgtct ttcccccgag
      901 gccatgcgca cgcttcgtgc cgtctccgaa atgcgaaacc gtcccgccga ggacgttctt
      961 cgtgaggagc ttcgcggcta catcgccgac aagcttccgc tgcccgacgt cgaggccatc
     1021 atccacgcga tgggcgagcg cttctatgcg ctcggctatg cgtgcggcac cgccaagcgc
     1081 ttcctgagga agctcagggg cgaatgaccc gcagtccttc aatgaaaaga cggcccgaaa
     1141 ccttgcggtt ccgggccgtt gtatttttca gggcattcag tcgcctgatt tcaaaagctt
     1201 cttcttgagg ccgtcgtcga tggcattgtc gccaagccag atggctagca cggcgttgta
     1261 gagcgcgcgg ccgccgccga tgggctcgcc cacgggcttg tcattcagat ggactgtggt
     1321 gcccttgtcg ggcgaatagt cgaagtcgat gatgtcgccc gttttcacgt cgccgatcga
     1381 cctcatgagg ctgcgcaggg cctcaagtcc ggcggaaatc gccttttcct tggcaggctg
     1441 gaggttcgcc ttgatgccgt cgttgagcga gtcgatgaag ctcgcgcagg agacgtcgcg
     1501 tagcaggccg agacgaacgc gggcagcgct gttctgggcc atgacctcgg tgttcctgcg
     1561 ggcgggcgcc ttcaggtaga gccctgccgt gtagaccttg aagaagattt tcgtgcgaag
     1621 gcccgcaccg ttgagcaaaa gttgctcgcc gttgacgttc gcctgcggct cgtaggtgat
     1681 ggattcgatc ttgaggggct ggccggcgaa ggcgatgcca agcgtgagcg tcatgcaggc
     1741 ggccgcggcc agtctcttga gaatgtgggt catgggcttt cctgatgtga aaaaggaaca
     1801 cgcagaatcg tgccaccgcc cgcgtgcgaa cgggaaaaaa ccgcaaacga ctttgtctca
     1861 agagttttca gtcttcccgg ccttcgcgga ctcggcggcc tttcgggcct cgatgcgctt
     1921 ctggcgcttc ctgccgcgca cgaccgaact ggtttgcagc gccacggata gtgcgatgag
     1981 tccgatgcct gcgtagaagg aaggaccgag gtcgcgggcc tcgccgaaga tgaccatggc
     2041 gaggatgatc gagtagatcg gctcgaggtt gtaggagagg ttgaccgtga aggccgagat
     2101 atgctcgagc gactggatct gcaggatgta catgaagagc gtgcagaaga tcgcgaagat
     2161 cggcagatag agcgtgtcgg gccaggtcgg gatgaagggc tcgggcggca tgagcgagac
     2221 gtagatgggg atcgcaacgc aggagaaggc gaagccgccg agtagctgcc agctcatgac
     2281 ggtgacgctc gagtacttgg ctcggtagcg cttgaagtag acggccagaa gcgccgccgc
     2341 tgcggcgccc acgaggccga gagcaatgcc gaggcggtac tgcgcgtcga agtggaagat
     2401 gagcgagatg ccgaagatcg tcacgagcga gaaaagcatc tcgcgcagcg agatgcgggc
     2461 cctgttcatc agcggctcga tgagcgccgt gaaaaagccg atcgacgaga aggccacgac
     2521 gccgatgctc acgttcgagg ccttgatcga ggcatagaag agcgtccact gcacggcgag
     2581 aagcgcgccc acgcccatga tcgcgatcct gtcgcgcggc gtcaccttct caaggcgctt
     2641 cgtcagccag gcccagagcc acaggcagcc gcccgcaatc acgatgcgcc agaagacgat
     2701 gaggccgggc gagagcatca tgagcttgcc gaagacgccg gtccagccgg ccagaagaat
     2761 ggagatgtga agctgaagga aggccttttt cacggcgcac cgccaggagg gagactgttt
     2821 gaagattggg aacaggtcgg cttttcgccc cttttcaagg ctctgccggc cgcgcctatg
     2881 ctaccgcaag aagaaggaac ttgccatgcc ggacacacgg cccgggcatg acgttttccc
     2941 gcatcttctt ccgcctcatc cttagctgat cgtcaagata cgtacagctc ttttgataat
     3001 agaaacttag ctagctaatt gtgaggtttc ggcggtatcg tttctcgaaa cgctacgaaa
     3061 agctccggtc ggaacttgcg gagatgcttc gtctttccgc cgccacgcga aagcgcgagc
     3121 acggtgaagt ctgcaactgg cttctcggcc atacaggcca catcattgtg gaggacaaca
     3181 gttacaaggc gttccagagg ggacggttcg gcaagaccat cgggcgacat gcgccggcgg
     3241 cgttatacgc tcaactcgca agcaaggctg aaagcgccgg tttgttggta gaagtcgtat
     3301 cgccaaagaa ggtcaagccc acgtagcaca atctgctgac gggtcaaatt gtcaagcacg
     3361 aattttggga gcgccgcgta cgattgggtg aagatgacga cgaccgttgg atcgaccgcg
     3421 atgcggcggc ttgtctgaat ctgttgtacg ccgatcttga gaaacaaacc tacgccccgg
     3481 aacggatccg tgaggccgtt ccagctggag taacagccag gcttgacgcc ggtgtcgtag
     3541 tcattcaagc ccgagaagga atcaccgagc gcgagttcgg gaggttccga cggcggggga
     3601 ttccgtcctc caccgtacaa gggc
//
LOCUS       DBMDTI010000201         3614 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5895_length_3614_cov_2.843723, whole genome shotgun sequence.
ACCESSION   DBMDTI010000201 DBMDTI010000000
VERSION     DBMDTI010000201.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5895_length_3614_cov_2.843723"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="ACZPB6_08360"
     CDS             complement(<1..186)
                     /locus_tag="ACZPB6_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR1184516.1"
                     /translation="MTKTPSRKHRPTAEAAAPLITGRVTAGHGRHYFVTTENGAVLEA
                     HRRGKKGDVVVGDVVECT"
     gene            complement(250..1602)
                     /locus_tag="ACZPB6_08365"
     CDS             complement(250..1602)
                     /locus_tag="ACZPB6_08365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR1184517.1"
                     /translation="MSLHATFIEHTFVSILALYVTLECAVTILQTRAAEKSCGRVPEG
                     FEKKLTLAGIRKAADYTGELAQANLLLTVVGAGFALFMTFGNGLNVFTAVIETIMGPG
                     IPADWVLISLIVLLMLLIELPFGWWARYRVKERYGYMREPRMRWLSRTLSEALWGWLV
                     FMPVSAALLTIFEYAGGDWWKLAWGVWLVYLLWRWKLSSVYGVFWKRRSRPYANAETR
                     EAVRESLHRQGITMTEMVVMTRPASWDHSNIVLSGWGLRRRVIVFAHVAHLLRKDEIV
                     ALAAHEAAHVRHFHGVLRLLINVAISYIFCWLAGWGATHVQFFEGFNYSPMLTLDMPG
                     THAGSVCAVALVVFPMLLYPLSPLLNLAARLMQYDADLHAARIAGLDPMISALVKLHK
                     DTTTSLSPSLVYSLFHYKRPHPGMRVANLQRLKRLSAPGGRLEHLFPKLAEPRAPEAD
                     "
     gene            1735..2304
                     /gene="orn"
                     /locus_tag="ACZPB6_08370"
     CDS             1735..2304
                     /gene="orn"
                     /locus_tag="ACZPB6_08370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HIR1184518.1"
                     /translation="MTNDIERDPAYSDDNLIWIDMEMTGLQPEVNRVIEVAAIITDKN
                     LNILHKGPVVAVHQSDEVLDGMDAWNTDTHTRSGLVARVKESTIDEAECEKIFLETFR
                     RFVPQGKSPMCGNTIGQDRRFMARWLPNLEEYFHYRYLDVSTLKELTRRWAPEKTRNI
                     QKATKHQALSDIEESIEELQYYRREILKI"
     gene            2652..3449
                     /locus_tag="ACZPB6_08375"
     CDS             2652..3449
                     /locus_tag="ACZPB6_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR1184519.1"
                     /translation="MFGFFEKKEAHVDEVKAPAFVEWKGWRKFKVMNRKQECVDTTTF
                     TLMPADEQPLPAFRAGQGVALRLEGFEPRAYTLCSTPDEGCYRITIKVLDNDRGALSA
                     QLAQCLHVGDEIEVSEPAGGFVLDDRETPVVMIAEGIGITPMVAMLSEIATDHPLRRV
                     HLIYSTKNGEHFPLQEETKNLIKLIPDSGMAVGYTEPRFDEHVGQDYQFTGKLMPATL
                     RHACMDAESDVYLCGSEIFVEYVRNIILQYRTIPADHIHTQVFVRTR"
BASE COUNT          742 a         1070 c         1184 g          618 t
ORIGIN      
        1 ggtgcactcg acgacgtcgc ccacaacgac gtcgcccttc ttgccgcggc ggtgggcttc
       61 aagcacggcg ccgttctccg tcgtcacgaa gtagtggcgg ccgtggcctg cggtgacgcg
      121 gcctgtgatg aggggggcgg cagcttcggc ggtcggacgg tgcttgcggg acggagtttt
      181 ggtcatggga cagtagctga ggttcgtgag cggacgtgag ccgcgaagaa aggttgctgg
      241 tgaggtagtt tagtccgctt cgggcgctcg gggctcggcg agcttcggaa agaggtgctc
      301 gagccggccg ccgggcgccg acaggcgctt gaggcgctgc aggttcgcaa cgcgcatgcc
      361 ggggtgcgga cgcttgtagt gaaagagcga atagacgagg gacggcgaga gagatgtcgt
      421 cgtgtccttg tgcagcttca cgagggcgct gatcatcggg tcgagtcccg cgatgcgcgc
      481 ggcgtgcagg tccgcgtcgt actgcatgag gcgtgcggcg aggttgagca ggggcgagag
      541 cgggtagaga agcatgggga agacgacgag cgcgaccgcg cagacgctgc ccgcatgggt
      601 gccgggcatg tcgagcgtga gcatcggcga atagttgaaa ccctcgaaga actggacgtg
      661 cgtggcgccc cagcccgcga gccagcagaa gatgtaggag atggcgacgt tgatgaggag
      721 gcggagcacg ccgtggaagt gacgcacgtg tgcggcctcg tgggcggcga gcgcgacgat
      781 ctcgtccttc ctgagcaggt gcgcgacgtg cgcgaagacg atgacgcggc gcctgaggcc
      841 ccagcccgag agcacgatgt tggagtggtc ccaggaggcg gggcgggtca tgacgaccat
      901 ttcggtcatg gtgatgccct gacggtgcag gctctcccga acggcctcgc gggtttcggc
      961 gttcgcatag gggcggctcc ggcgcttcca gaagacgccg tagacgctcg agagcttcca
     1021 gcgccagagg agatagacga gccagacgcc ccatgcgagc ttccaccagt cgccgcccgc
     1081 gtattcgaag atggtgagaa gcgcggcgga gacgggcatg aagacgagcc agccccagag
     1141 ggcctctgaa agggtgcgcg agagccagcg catgcgcggc tcgcgcatgt agccgtagcg
     1201 ttccttgacg cggtagcggg cccaccatcc gaagggaagc tcgatgagga gcatgaggag
     1261 gacgatcagg ctgatgagga cccagtcggc gggaatgccc ggacccatga tcgtctcgat
     1321 gacggccgtg aagacgttga ggccgttgcc gaacgtcatg aagagcgcga agcccgcgcc
     1381 caccacggtg agcagaaggt tcgcttgggc gagttcgccc gtgtagtccg ccgcctttcg
     1441 aatgcccgcg agcgtgagct ttttctcgaa gccctcgggc acgcgtccgc acgacttctc
     1501 ggccgcgcgg gtctgcagga tcgtgaccgc gcactcgagc gtcacgtata gcgcaaggat
     1561 gctgacgaag gtgtgctcga tgaaggtggc gtgcagggac atgggacgga ctgcgcggac
     1621 gggcgtgatg aaagggacga taatcctata ttatggcgga tcggcgcccc cgcaggaaag
     1681 acgcgggggg gcggagcgcc tctgctcgaa cctcttttgg acaaacgact acccatgacg
     1741 aacgacattg aacgagatcc cgcgtattcg gacgacaacc tgatctggat cgacatggag
     1801 atgaccgggc ttcagcccga ggtgaatcgc gtgatcgagg tcgcggcgat catcaccgac
     1861 aagaatctga acatcctgca caagggcccc gtggttgccg tgcatcagag cgacgaggtc
     1921 ctcgacggca tggacgcctg gaacaccgac acgcacaccc gctccggact cgtggcccgc
     1981 gtgaaggaaa gcacgatcga cgaggccgag tgcgagaaga tcttcctcga gaccttccgc
     2041 cgcttcgtgc cgcagggcaa gagcccgatg tgcggcaaca cgatcggcca ggaccgccgc
     2101 ttcatggcgc gctggctccc gaacctcgag gagtacttcc actaccgcta cctcgacgtc
     2161 tcgaccctca aggagctcac ccgccgctgg gctcccgaaa agacgcgcaa catccagaag
     2221 gccacgaagc accaggccct ctccgacatc gaggaaagca tcgaggagct tcagtactac
     2281 cgccgcgaga tcctgaagat ctgacggacg gacctcatct gaattgacga agacggctgt
     2341 ctcccttgcg ggggacggcc gtttttcttt gattttttca tttcaaacgc tgcgcaggga
     2401 ggacttcccg agtgcagtgt ctgcgcacga aaggggttga taagggttga ttcctatacc
     2461 gaaaagggta gtggatctgt aaggggtagt ctggtttatc taaaagatca acaaaagtcg
     2521 atgcgaaagg attaggtggt agtagttagg tgtatgctgt taaacaacag gacgccgaga
     2581 ccgggcgtca gctgaaagcc atttgcaaag cgctcgaaac gcctgcgcaa gaattaaaaa
     2641 ggagaagcat catgttcgga tttttcgaaa agaaggaagc ccacgttgat gaagtcaagg
     2701 ccccggcctt cgtcgaatgg aagggctggc gcaagttcaa ggtgatgaac cgcaagcaag
     2761 aatgcgtcga cacgaccacc ttcacgctga tgcccgccga cgagcagccg cttcccgcct
     2821 tccgcgccgg ccagggcgtg gcgcttcgcc tcgagggttt tgagccgcgc gcctacacgc
     2881 tttgcagcac gcccgacgaa ggctgctacc gcatcaccat caaggtgctc gacaacgacc
     2941 gcggcgccct ttccgcccag ctcgcccagt gcctgcacgt cggcgacgag atcgaggttt
     3001 ccgaacccgc tggcggcttc gttcttgacg accgtgaaac tcccgtcgtg atgatcgccg
     3061 aaggcatcgg catcaccccg atggtcgcga tgctctccga gatcgccacc gaccacccgc
     3121 tgcgccgcgt gcatctcatc tactccacga agaacggcga gcacttcccg cttcaggagg
     3181 agacgaagaa cctcatcaag cttattcccg acagcggcat ggccgtgggc tacaccgaac
     3241 cgcgctttga cgaacacgtc ggccaggact accagttcac gggcaagctg atgcccgcca
     3301 cgctgcgtca cgcctgcatg gacgccgaaa gcgacgtcta tctctgcggc tccgaaatct
     3361 tcgtcgagta cgtgcgcaac atcatcctgc agtaccgcac gattcccgcc gatcacatcc
     3421 acacccaggt gttcgtgcgc acccgctgat ctgaacggaa ttgataatcc cctcccaatc
     3481 gattggaagg ggattctggg ggccgtaacc cccccccccc attctagtcc ctacccaatt
     3541 cgggcagtag cgggctgcat caaacagccc tcatcggttt cgtctagctt tttcaagctt
     3601 ctgaaagcgg acgg
//
LOCUS       DBMDTI010000202         3586 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_5944_length_3586_cov_2.963271, whole genome shotgun sequence.
ACCESSION   DBMDTI010000202 DBMDTI010000000
VERSION     DBMDTI010000202.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5944_length_3586_cov_2.963271"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            291..2327
                     /locus_tag="ACZPB6_08380"
     CDS             291..2327
                     /locus_tag="ACZPB6_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR1184520.1"
                     /translation="MEHSLPLISTLVIAFSLALVFGFLAERLFKAPPLVGYLLAGIAA
                     GKYTPGVFADAALAQQLSEIGVMLLMFGVGLHFSVRDLMNVKGIAVPGAVLQMFIATL
                     LGGLVAHFIWDWHWGDALVLGMCLSCASTVVLLKALEVRGILQSMDGQIAVGWLVVED
                     VATVLILVLLPPLAGLLGSDAAPGITGKDIAIQIAITIGNVIAFIAVMMLVGRRVLPW
                     IMAQVARTGSRELFTLFVLAAAVGIAYGASAIFNVSFALGAFFAGMVMRESKFAHRAA
                     TESLPLQDAFSVLFFVGVGMLFDWHIIIEAPLSVLTVLLIIMLGKGLAAFGLVYVLRY
                     PLHTAITVAASLAQIGEFSFILVGQAKGLGLATDYTVNLIVGAAILSIALNPVVFSFI
                     PAIGRELTSRWAWARAAALREAPFERVSNETPAEKLHNQIVITGKSRLLPPLATELHK
                     AGAAVVAIVQDHDLATELDKLDVTVIVGNPADPQAMVAAHLKNAGMLMILDNSTTSLR
                     IAEAARAVKDDTPITIVAPDPGIWTDVPGIDGVRFVSSYESAAEKISVDVNTAFAENA
                     PIGTIINTKLAEATAREEERTENTEDKDRDETAAPEQASVSVPQSETTGVAPAAPSDA
                     SIELQASSAPSAEAVESVSLSEGSGAETAVPTEEKAAGETKDRPGIFSFLRRKQ"
     gene            2415..>3586
                     /gene="coaBC"
                     /locus_tag="ACZPB6_08385"
     CDS             2415..>3586
                     /gene="coaBC"
                     /locus_tag="ACZPB6_08385"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR1184521.1"
                     /translation="MTTLRSVTLAVTGGIAAYKCCELVRGLKKAGIDVHVAMTEHAAA
                     FVGPITFEALTGHPVALTEWAPGPQGSMPHIELNRSNDLLIVMPATANIIAKAAHGIA
                     DDLVSTMIAARRQPVLFVPAMNRFMWENPANLRNVEQLRRDGALFAGPACGFQACGDV
                     GAGRMVEPSEVLDLLPGLLAPKSLSGRRVVITAGPTFEPIDDVRGITNKSSGLQGYEI
                     ARASRDAGADVTLVSGPVHLPTPFGVKRVDVTTAAEMLASVEEALKANGADVFIGVAA
                     VADWRIATAVSGKMKKTDGRPPELRFEENPDILRTVGTRSDVKLKVGFAAEAENLEAY
                     ARGKCISKHADLIVGNLARTAIGSPDNCVLLVTPESAEAFGPASKREVALKIVSRIA"
BASE COUNT          651 a         1284 c          977 g          674 t
ORIGIN      
        1 cgccttcaac gtcgctgacg tcgccatctg cggcggcgcc ttcttcctga tcgtcgacga
       61 actgagaagc ggacgcagat aagtcatttc gggcatcacc gctcctcaat gagggactgg
      121 aaaccaaacg gattccagtc cctttccttt tacctttcac ctcccgaaaa cgcatcccgc
      181 acaagttttt tcagcttttt cacccccatt gaaacggcgc tcaaagtaca atcacacggc
      241 ttgaatatcc cgttcctgcc ccgccacccg tacacgcgtc aacgcctcct atggaacact
      301 cccttcccct catttcaaca ctcgtcatcg ccttcagtct ggcgctcgtc ttcggctttc
      361 tggccgagcg cctcttcaag gcacctccgc tcgtcggcta ccttctggca ggcattgctg
      421 cgggcaagta cacgccgggc gtcttcgccg acgcggccct tgcccagcag ctctccgaaa
      481 tcggcgtcat gctgctgatg ttcggcgtcg gcctccactt ctccgttcga gacctgatga
      541 acgtcaaggg catcgccgtg cccggggccg tgctgcagat gttcatcgcc accctgctcg
      601 gcggcctcgt cgcgcacttc atctgggact ggcactgggg ggatgcactc gtgctcggca
      661 tgtgcctctc gtgtgcatcg acggtcgtgc ttctcaaggc gcttgaagtg agaggaatcc
      721 tgcagagcat ggacggccag atcgccgtcg gctggctcgt cgtcgaggac gtcgccaccg
      781 tgcttatcct cgtgcttctg ccgccgctcg ccggccttct cggctccgac gccgcgccag
      841 gtattacggg taaggacatt gcgatccaga tcgccatcac gatcggcaac gtcatcgcct
      901 tcatcgccgt gatgatgctc gtcggccgcc gcgtcctccc gtggatcatg gctcaggttg
      961 cccgcacggg ctcccgcgag ctcttcacgc tcttcgtgct tgccgcagcc gttggcatcg
     1021 cttacggcgc ctccgccatc ttcaacgtga gcttcgccct cggcgccttc ttcgcaggca
     1081 tggtgatgcg cgagtcgaaa ttcgcccaca gggcggccac cgaatcgctc ccgctccagg
     1141 acgccttctc cgtgctcttc ttcgtcggcg tgggcatgct ctttgactgg cacatcatca
     1201 tcgaggcccc gctctccgtc ctcaccgtgc tgctcatcat catgctcggc aagggtctcg
     1261 ccgccttcgg cctcgtctac gtgctgcgct acccgctgca cacggccatc acggtcgccg
     1321 cctcactcgc acagatcggc gaattttcgt tcattctcgt cggtcaggcc aagggcctcg
     1381 gcctggcgac cgactacacg gtcaatctga tcgtgggcgc cgccattctg tcgatcgcac
     1441 tcaaccccgt cgtcttcagc ttcatccccg ccatcggcag agagctgacc tcccgttggg
     1501 cctgggcgcg cgccgccgcg ctgcgtgaag cacccttcga acgcgtctcc aacgaaacgc
     1561 ccgccgaaaa gcttcacaac cagatcgtca tcacgggcaa gagccgcctg ctgccgccgc
     1621 tcgccaccga gcttcacaag gcaggcgcgg ccgtcgtcgc catcgttcag gatcatgacc
     1681 tcgccaccga actcgacaag ctcgacgtca cggtcatcgt cggcaacccc gccgatccgc
     1741 aggccatggt cgctgcgcat ctcaagaacg ccggcatgct gatgatcctc gacaactcga
     1801 ccacttcgct gcgcatcgcc gaagccgccc gagctgtcaa ggatgacacg cccatcacga
     1861 tcgtggctcc cgatcccggc atctggaccg acgtgcccgg catcgacggc gttcgcttcg
     1921 tgagcagcta tgaaagcgcc gccgaaaaga tctccgtcga cgtgaacacc gccttcgccg
     1981 aaaacgctcc catcggcacc atcatcaaca cgaagctcgc cgaggcgacg gcccgtgagg
     2041 aggaacgaac cgagaacacc gaagacaagg acagggatga aaccgccgct cccgaacagg
     2101 cttccgtctc cgtacctcag tccgagacta caggcgttgc gcccgcagca ccctccgatg
     2161 cctccattga gcttcaagcc tcttccgctc cctccgccga agcagtcgaa tccgtttccc
     2221 tctccgaagg atcgggagcc gaaaccgccg tgccgacgga agagaaggca gcaggcgaaa
     2281 cgaaggatcg tcccggcatc ttcagcttcc tgcgtcgcaa gcaatgactg attgctccct
     2341 gtcgcttccg ctaaaatgaa cggccgcggc cttcaccatc cgtgaaggcc gcgccttttc
     2401 ttatggagat tctcatgacg acactgcgaa gcgtcacgct ggccgtaacg ggcgggattg
     2461 ccgcctacaa gtgctgcgaa ctcgtgcgcg gtctgaaaaa ggccggcatc gacgtacacg
     2521 tcgccatgac ggagcatgcc gccgccttcg tcggccccat cactttcgag gcgctgacgg
     2581 gccatcccgt cgccctcacg gaatgggcgc cgggtcctca gggctccatg ccgcacatcg
     2641 agctcaaccg ctcgaacgac ctcctcatcg tgatgcccgc tacggcgaac atcatcgcca
     2701 aggccgctca cggcatcgca gatgacctcg tctccaccat gatcgccgcc agacgccagc
     2761 ccgtcctgtt cgtgcccgcc atgaaccgct tcatgtggga gaatcccgcg aatctgcgca
     2821 acgtcgagca gctccgaagg gacggcgccc tgtttgcggg ccccgcctgc ggctttcagg
     2881 cctgcggcga cgtcggtgca ggacgcatgg ttgagccttc ggaagtcctt gacctgctcc
     2941 ccggactgct tgcacccaaa agcctcagcg gaagacgcgt cgtcatcacg gcggggccca
     3001 ccttcgagcc catcgacgac gttcggggca tcaccaacaa aagttccgga cttcagggat
     3061 acgaaatcgc ccgcgcctcc cgtgatgcgg gcgccgacgt gacgcttgtc tcgggtcccg
     3121 ttcaccttcc gacgcccttc ggcgtcaagc gtgttgacgt cacgacggcc gcggaaatgc
     3181 tcgcctccgt cgaagaagcc ctcaaggcaa acggcgcgga cgtcttcatc ggcgtcgccg
     3241 ccgtggccga ctggcgcatc gccaccgccg tcagcggaaa aatgaagaaa acggacggcc
     3301 gcccccctga gcttcgcttt gaagaaaacc ccgacattct cagaactgtc gggacgcgca
     3361 gcgacgtaaa gctcaaggtc ggctttgctg ccgaggctga aaacctcgag gcctatgcca
     3421 gaggcaagtg catctcaaaa cacgcggacc tgatcgtggg caaccttgca cgcacagcca
     3481 tcggaagccc cgacaactgc gtgctccttg taacgccaga gagcgccgaa gccttcgggc
     3541 ctgcctccaa gcgggaggtt gcactgaaaa tcgtcagccg catcgc
//
LOCUS       DBMDTI010000203         3558 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6012_length_3558_cov_3.296500, whole genome shotgun sequence.
ACCESSION   DBMDTI010000203 DBMDTI010000000
VERSION     DBMDTI010000203.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6012_length_3558_cov_3.296500"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(101..1108)
                     /gene="rfbB"
                     /locus_tag="ACZPB6_08390"
     CDS             complement(101..1108)
                     /gene="rfbB"
                     /locus_tag="ACZPB6_08390"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR1184522.1"
                     /translation="MLLVTGGAGFIGSNFITEWFWSHDESILTLDALTYAGNKSNLRD
                     VEHDPRHRFVEGNILDEALVADLFDRFRPRAVVHFAAESHVDRSISGPQVFVDTNVRG
                     TTVLLGVAHRYWLTLDDEEKAAFRFINISTDEVYGSMTADTPPNTEATPFDPSSPYSA
                     SKAAADQFGRAYFRTYGLPVVTVRCTNNYGPRQYPEKLLPFMLTRALTGKTLPVYGDG
                     EQIRDWIHVHDFCRALRMILEKAAPGDIYNVGASNEMSNLKFLQSAMSVLDELSPRAD
                     GRPHADAIVHVHDRPGHDRRYALDAGRLRQTLGWKPMIPFDEGLRATVRWYLKQCVSA
                     F"
     gene            1219..2679
                     /gene="rng"
                     /locus_tag="ACZPB6_08395"
     CDS             1219..2679
                     /gene="rng"
                     /locus_tag="ACZPB6_08395"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HIR1184523.1"
                     /translation="MVEHILINCTPREVRVAIVVDGVLQDLFIERDCARGLVGNVYLG
                     KVVRVLPGMQSAFVDVGLERTAFLHVADIAEAHREGEKPAPIETILHEGQSLMVQVAK
                     DPIGTKGARLTTTVSLAGRKLVYLPRDNHIGVSQRIEDAELRESLRAMMTELRPETEK
                     GGYIIRTSAEEGATEQEFKDDMAYLGRLWTDIHELARRSTGPKLLYQDLSLAQRVLRD
                     MVHQDTQTVEVDSRENYEALLNFASRFVPAAKDRLTLYRGERPLFELHGIETEIEHAL
                     ERRVDLKSGGYLVFDQTEAMTTIDVNTGGYVGKRDFSDTVFKTNLEAAQTIARQLRLR
                     NLGGIIIVDFIDMSCDEHREAVLAELRRSVSTDRTRMTVSNFTELGLVEMTRKRTRES
                     LAHVLCEPCPMCGGRGEVRTARTVCYSIMREIVRLCKQYQETKEFHIQASQTVIDMLL
                     EEESQALELLQVFVEKPVHLEVEPSYTQEQFDVILA"
     gene            2690..>3558
                     /gene="sbcB"
                     /locus_tag="ACZPB6_08400"
     CDS             2690..>3558
                     /gene="sbcB"
                     /locus_tag="ACZPB6_08400"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HIR1184524.1"
                     /translation="MTESTTFYWYDFETFGLDCRRDRPAQFAGIRTDMDLNPQGDGDV
                     FYSRPSGDYLPSPESCLLTGMTPQICEERGIPESEFAGEVWSRLNRPGTISIGYNASG
                     FDNEVARFLFWRNFLDPYSHQHRNGCSCWDLYPLVCAVWALRGDGIEWPLRKDVDPES
                     NDEKSVSFRLECLSKANGIVHAHSHEALSDAMATLGLAALIRKTEPRLWKWALENRSG
                     EAVKAAVTKGPVVWISPKFGQARGFLRIAARIPFPGESGNSALMWDLSVDPLIVTKLS
                     DEEIKSRLFARRE"
BASE COUNT          717 a         1031 c         1127 g          683 t
ORIGIN      
        1 gtgacctacg cccacttgtg gcgaagcgaa tggccgacgc cgctcatgct ggtcggtctg
       61 gggcttcttc tgatgggggt tacagcttcc ctcaaggttt tcagaaggcc gagacgcact
      121 gcttcaggta ccacctgacc gtagcgcgca gcccttcgtc aaaggggatc atgggcttcc
      181 atcccagcgt ttgacgcagg cgtcctgcgt ccaaagcgta gcggcggtcg tgccccggac
      241 ggtcgtggac atgcacgatc gcatccgcgt gaggacggcc gtcggcgcgc ggcgaaagtt
      301 catcgagcac cgacatcgcg ctctgcagaa acttcagatt gctcatttcg tttgaggcgc
      361 cgacgttgta gatgtcgccc ggtgccgcct tttccaaaat cattcgcagt gctcgacaga
      421 agtcgtgtac atggatccag tcgcggatct gctcgccgtc gccgtaaacg gggagcgtct
      481 tgcccgtcaa ggcgcgcgtc agcatgaagg gcagaagttt ttccgggtac tgtctgggac
      541 cgtagttgtt ggtgcatctc accgtgacga cgggcaggcc gtaggttctg aagtaggcgc
      601 gtccgaactg atcggccgcc gccttgctcg cggagtacgg actgcttgga tcgaagggcg
      661 tggcctccgt gttgggcggc gtgtccgcgg tcatggagcc gtagacctcg tcggtggaga
      721 tgttgatgaa gcgaaaggcg gccttctcct catcgtcgag cgtcagccag tagcggtgtg
      781 caacacccag aagaaccgtc gttcccctga cgttcgtgtc gacgaacacc tgagggccgg
      841 aaattgaacg gtcgacgtga ctttcggcgg cgaagtgcac gacggcgcga ggacggaagc
      901 ggtcgaaaag atcggccacc aacgcttcgt ccagaatgtt tccttcgaca aagcgatgtc
      961 tggggtcgtg ctcgacgtct ctgaggttgg acttgtttcc tgcataggtc agggcgtcga
     1021 gcgtcagaat gctttcgtcg tgtgaccaga accattccgt gatgaagttt gagccgatga
     1081 agccggctcc gcctgtgacc aacagcatgc gtgaaaatcc gagagaagag cgccggcttt
     1141 caaagcgggt gcggcaaacc cactacaatg tgagcagtca attttgagcg caacgtcgca
     1201 ggcaggcaag gagccgccat cgtggaacat attctcatca actgcacgcc ccgtgaagtg
     1261 cgtgtcgcca ttgtagtgga tggcgttctt caggatctct tcatcgagcg cgactgtgcg
     1321 cgcggcctcg tggggaatgt ttatctcggc aaggtcgtcc gtgtgctgcc tggcatgcag
     1381 agcgccttcg tggacgtggg gctcgaacga accgccttcc ttcacgtggc cgacattgcg
     1441 gaggcgcatc gcgagggcga gaagcccgcg cccatcgaga cgatccttca cgaaggccag
     1501 tccctcatgg tgcaggtcgc caaggatccc atcggcacca agggcgccag actgacgacg
     1561 accgtttcgc tcgcgggccg caagctcgtc tatctgccca gagacaacca catcggcgtc
     1621 tcccagcgca ttgaggacgc agagcttcgc gaatcacttc gcgccatgat gacggagctt
     1681 cgtccagaaa ccgaaaaggg cggctacatc atccgcacga gcgccgaaga gggcgcgacc
     1741 gagcaggagt tcaaggatga catggcctat ctaggccgtc tgtggaccga cattcacgag
     1801 ctcgcgaggc gaagcaccgg accgaagctc ctctatcagg atctgtcgct tgcccagcgc
     1861 gtgctccgcg acatggttca tcaggacacg cagaccgtcg aggtcgacag ccgcgagaac
     1921 tacgaggccc tgctgaactt tgcaagccgt tttgtgcctg cggctaagga caggctcacc
     1981 ctctacaggg gcgagaggcc gcttttcgag ctgcacggca tcgagaccga aatcgagcat
     2041 gcgcttgagc gcagggtcga cctgaagagc ggcggctatc tggtcttcga tcagacggag
     2101 gccatgacga cgatcgacgt caatacgggc ggctacgtcg gcaagcgaga cttcagcgac
     2161 acggtcttca agacgaacct cgaggctgcg cagaccatcg cgcgccagct gcgcctgaga
     2221 aatctcggcg gcatcatcat cgtcgacttc atcgacatga gctgcgacga gcaccgcgag
     2281 gcggttcttg ccgaacttcg ccgttcggtc tcaaccgacc gcacccgcat gacggtgagc
     2341 aacttcaccg agctcggtct cgtcgagatg acgcgaaagc gcacccgcga gtcgcttgca
     2401 cacgtcctct gcgaaccttg tcccatgtgc ggcggcaggg gagaggtccg cacggcgcgc
     2461 accgtctgct actcgatcat gcgtgagatc gtgaggctct gcaagcagta tcaggagacg
     2521 aaggaatttc acattcaggc gagccagacc gtcatcgaca tgctgctcga ggaggagtcg
     2581 caggcgctcg agcttctgca ggtcttcgtc gaaaagcctg tgcatcttga ggtggagccg
     2641 agctacaccc aggaacagtt tgacgtgatt ctcgcctgag gaccttcgca tgacggaaag
     2701 caccaccttc tactggtatg acttcgaaac cttcggcctc gattgccgca gggacaggcc
     2761 cgcacagttt gcaggcattc ggacggacat ggaccttaat ccgcagggag atggggacgt
     2821 gttttattcc aggccttcgg gagactatct gcccagccca gagagctgtc ttctgacggg
     2881 gatgacgccg cagatctgcg aggaaagggg cattcccgag agcgagtttg cgggcgaagt
     2941 ctggagtcgg ctcaacaggc ccggaacgat cagcatcggg tacaacgcgt ccggcttcga
     3001 caacgaggtc gcgcgcttcc ttttctggcg caattttctc gatccgtatt cccatcagca
     3061 ccggaacgga tgttcatgct gggacctgta cccgcttgtc tgcgccgtct gggcgcttcg
     3121 gggcgacggc atcgagtggc cgctcagaaa ggatgtggat cccgaaagca atgatgaaaa
     3181 gagcgtcagc ttcaggctgg agtgtctgag caaggccaac gggatcgtgc acgcacattc
     3241 gcatgaggct ttgagtgacg caatggccac gctgggactg gcggcgctca tccgcaaaac
     3301 ggaacccaga ctttggaagt gggcgctgga gaaccgcagc ggggaggctg tgaaggcggc
     3361 cgtgacgaag gggcccgtcg tctggatctc tccgaagttc gggcaggcaa gaggctttct
     3421 gcgcatagcc gccagaattc cgttccccgg cgagagcggc aattccgcgc tgatgtggga
     3481 cttgtccgtg gatccgctga tcgtgacgaa gctgagcgac gaagagatca agtcacgcct
     3541 tttcgcccgc agggagga
//
LOCUS       DBMDTI010000204         3547 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6025_length_3547_cov_4.308183, whole genome shotgun sequence.
ACCESSION   DBMDTI010000204 DBMDTI010000000
VERSION     DBMDTI010000204.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6025_length_3547_cov_4.308183"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="ACZPB6_08405"
     CDS             complement(<1..1091)
                     /locus_tag="ACZPB6_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014385.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR1184525.1"
                     /translation="MKRSVLGLLIGALFLSGCAVQMPDMKTMAEEAAPGAWSVSIETQ
                     APADPTEFWQSWNDPKLSALVEKARSANADVLTALASLRTARAGLSEAQHALFPEATI
                     GGDASHRHADGGSSDSFSASADMKWSWNLAGADLSNETARRYEALASEISVADAQALV
                     EAETAQAYINLRSTQVLLQIAEDTVRNYEESAEIAGWRAGAGLSSASEAEDALSQVAS
                     ARAAIPQLKATEATYRNALARLTTIPADKLDLGTGSVPQAPIRLTASIPAETLMKRPD
                     IRSAQASLLSAGADLRSARESYFPTLSLSGSLGTQAATLGALGASGTGIASLIGALSM
                     PVLNWGSLAAQEERAAASLDKAKANYLSV"
     gene            1328..2020
                     /locus_tag="ACZPB6_08410"
     CDS             1328..2020
                     /locus_tag="ACZPB6_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1184526.1"
                     /translation="MFKHPRPILIVDDDVELVTLLVDYLQLEGFAPVAAHNGTEALEL
                     LSRQEFEIVVLDVMMPGMSGVEVLKRIREKSQLPVLMLTAKGDPVDRIIGLELGADDY
                     VPKPCPPRELAARLNAILRRTANMPSAIAPVVAGPLTINPSRREATANGIVIDLTGTE
                     LTLLEVLARKVGTPVPKDEIYQKVLGRAMQRYDRAIDVHVSSIRHKLAAVLGTSVAIE
                     SIRGVGYQFVVR"
     gene            2070..3461
                     /locus_tag="ACZPB6_08415"
     CDS             2070..3461
                     /locus_tag="ACZPB6_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1184527.1"
                     /translation="MLNRLFNTLFGKLFAGFCLVILLTAAGVWMAAYSAQSQQNEDIG
                     RIEWRFIAHKSVDSAVGIYEVAGRDGLISWLRNERLNTNPIVFVLDSSGREISGRKLP
                     DKAYESLEAIRKLTDDPNGPPEKRLPVRTVEIDNEPCEFFAVRTVAFPVRLIPPELHH
                     FPIALTLSLAAFFTLLVSWLLARLYTRSLHTLDGAMRRFADGAFDTRTSEELARGDAE
                     VANLARVFDGMADKIESLITRQRRLFHDVSHEVRSPLARIEVALELARRDPARTQDSL
                     GRIEKEVGNINALVESLLTYARLENGANMTKSPVGLADILEGVADALGFEAQQKNVTV
                     KTVLEGDPVVDADGNLIARAVDNIARNSLRHAPEDSTILLSLSSDAESFIIRCRDEGP
                     GMPEEELAGMFSPFVRGANVRTGTGFGLGLAIARRSVTAHGGSITARNVSPHGLETEI
                     RLPKSIEIGCDEP"
BASE COUNT          643 a         1210 c         1045 g          649 t
ORIGIN      
        1 atgacggata ggtagttcgc cttcgccttg tccagcgatg cggcggccct ctcctcctgt
       61 gcggccagac tgccccagtt gagcacgggc atggacagcg cgccgatcag ggacgcgatg
      121 cccgtgccgc tcgcgccgag ggcgccgagc gtcgcggcct gcgtgccgag cgagcccgag
      181 agcgacagcg tcgggaaata gctttcgcgg gcgctcctga gatcggcgcc tgcagacaga
      241 agagaggcct gcgcgctgcg gatgtcagga cgcttcatca gcgtctcggc gggaatcgag
      301 gcggtcaggc gaatcggtgc ctgaggtacg gaacccgtgc ccagatcgag cttgtcggcg
      361 ggaatcgtcg tcaggcgggc cagtgcgttt cgataggtgg cttcggtcgc cttcagctga
      421 ggaatcgcgg ctcgggcgga ggcgacctgc gaaagcgcgt cctcggcctc cgaggcgctg
      481 ctcaggcctg cgcctgcacg ccagcccgcg atctcggcgc tttcctcgta gttcctgacg
      541 gtgtcctcgg cgatctgcag gagaacctgc gtcgaacgca ggttgatgta cgcctgagcc
      601 gtctcggcct cgacgagcgc ctgcgcatcg gcaacggaga tttcgctcgc gagcgcctca
      661 tagcgtctgg cggtctcgtt ggagagatcc gcacccgcga ggttccacga ccacttcatg
      721 tcggcggagg ccgagaaact gtcgctcgaa ccgccgtcgg catggcggtg cgaagcgtcg
      781 ccaccgatcg tggcctcggg aaagagcgca tgctgcgcct ccgacaggcc cgctctggcc
      841 gtacgcagtg atgcgagcgc tgtgagcacg tcggcatttg cgcttctggc cttctcaacg
      901 agcgccgata gcttcggatc gttccaggac tgccagaatt cggtggggtc ggcaggcgcc
      961 tgagtctcaa tcgagacgct ccaggccccg ggagccgcct cctcggccat ggtcttcatg
     1021 tccggcatct gcactgcgca gccagagagg aacagcgcgc cgatcagcaa gcccaggaca
     1081 gacctcttca tcgtcaacgg attcgtcatt gtttttctgc tgtatccggt ctttgggaaa
     1141 tttgaacgag aatcataaac ggttgagatc ctcactttca ttctcctaca gaccacaacg
     1201 gcgttccaca ggcttcgggt aaaggtttcg gaagagcacg taacaaactt ttgctcgccg
     1261 atttttgtta acatgagtca ttccattgtt ccaagtcacg gttcaagccg ttattttcgg
     1321 gtccgttatg ttcaagcatc cccgtcccat cctcatcgtc gacgatgacg tcgagctcgt
     1381 cacgcttctc gtcgactacc tgcagctcga gggcttcgct cccgttgctg cccacaacgg
     1441 caccgaggct ctggaactgc tttcaagaca ggaatttgaa atcgtcgtgc tcgacgtgat
     1501 gatgcccggc atgagcggcg tggaggttct caaacgcatt cgcgaaaaga gccagcttcc
     1561 cgttctcatg ctcaccgcca agggcgatcc cgtcgaccgc atcatcggcc tcgagctcgg
     1621 cgccgacgac tacgttccca agccctgtcc gccgcgcgag cttgccgccc gactcaacgc
     1681 gattctccgc cgcacggcca acatgccctc tgcgatcgcc cccgtggtgg ctggccccct
     1741 caccatcaac ccgagccgcc gcgaagccac ggccaacggc atcgtcatcg acctgacggg
     1801 caccgaactc acgctccttg aggtgctggc ccgcaaggtc ggcactcccg tcccgaagga
     1861 cgagatctac cagaaggtgc tcggccgcgc catgcagcgc tatgaccgcg cgatcgacgt
     1921 ccacgtctcc tcgatccgcc acaagctcgc cgccgtgctt ggaacgagcg tcgcgatcga
     1981 aagcatccgc ggcgtgggct atcagttcgt ggtccgctga tccgctgcat ccgccgactc
     2041 gccgtcccgc cgaaaagaag gagtagctca tgctcaaccg cctgttcaac acgcttttcg
     2101 gcaagctctt cgccggcttc tgcctcgtca ttctgctgac ggcggcaggc gtctggatgg
     2161 ccgcctactc ggcccagagc cagcagaacg aggacatcgg ccgcattgaa tggcgattca
     2221 tcgcacacaa gtccgtcgac agcgccgtcg gcatctacga ggtggctggc agggacggcc
     2281 tcatctcatg gctgcgcaac gagcgcctca acaccaatcc catcgtcttc gtgcttgaca
     2341 gcagcggccg ggaaatctcc ggacgcaagc tgcccgacaa ggcctacgaa agtcttgagg
     2401 ccatccgcaa gctcaccgac gaccccaacg gtccgccaga aaagcgcctg cccgtcagaa
     2461 ccgtcgagat cgacaacgag ccctgcgaat tcttcgccgt gcgcaccgtc gccttccccg
     2521 ttagactgat tccccctgag ctccatcact ttccgatcgc gctgaccctt tcgctcgccg
     2581 ccttcttcac gctactcgtc tcctggcttc tcgcccgcct ctacacgcgc agcctccaca
     2641 cgctcgacgg cgccatgcgc cgcttcgccg acggcgcctt cgacacccgc acctccgagg
     2701 aactcgcccg cggggacgcc gaggtcgcca acctcgcacg cgtcttcgac ggcatggcgg
     2761 acaagatcga gtccctcatc acaaggcagc gaaggctctt tcacgacgtg agccacgagg
     2821 tccgcagccc gctcgcgcgc atcgaggtgg cgcttgagct tgcgaggcgc gaccccgcac
     2881 gcacgcagga ctcgctcgga cgcatcgaga aggaggtcgg caacatcaac gcactggtgg
     2941 agtcgcttct cacctatgcc cgtcttgaaa acggcgccaa catgacgaag tcgcccgtcg
     3001 gtctcgcgga tatcctcgag ggcgttgccg acgccctcgg cttcgaggcc cagcagaaga
     3061 acgtgaccgt gaagacggtg ctcgaggggg atcccgtcgt cgatgcggac ggcaacctga
     3121 tcgcaagggc cgtcgacaac attgcgcgca actcgcttcg ccacgcaccc gaagactcca
     3181 cgatcctcct gtcgctctcc tcggacgcgg agagcttcat catcagatgc agggacgagg
     3241 gtcccggcat gcccgaagag gagctcgcag gcatgttctc gcccttcgtg cgcggcgcca
     3301 acgtccgcac gggcacgggg ttcggccttg ggctcgccat cgccagacga agcgtgacgg
     3361 cgcacggcgg ctccatcacg gcgcgcaatg tcagccccca cggtctcgag accgaaatcc
     3421 gtctgccaaa gtccattgaa atcggctgcg acgagccctg aacggccctg aaagcaaaaa
     3481 gccttgccgc gaaatcatcg gggcaaggct tttgtgtttt ggcgcaacta tacagtttgc
     3541 ccctttt
//
LOCUS       DBMDTI010000205         3543 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6034_length_3543_cov_1.942766, whole genome shotgun sequence.
ACCESSION   DBMDTI010000205 DBMDTI010000000
VERSION     DBMDTI010000205.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6034_length_3543_cov_1.942766"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="ACZPB6_08420"
     CDS             complement(<1..938)
                     /locus_tag="ACZPB6_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR1184528.1"
                     /translation="MTNYALPVNDKDISKAKTFYDLICWGGLLIVLLPVGIANVILGY
                     FMGDSPCTLCWGQRQEMAYIGVVALFMVRYGFKPKYLATMLVMAAVGLYMSFRHYGNH
                     AMRDVGQGFGLDVFGIHTQFWAEIVFWCVVFLFGLAVYLAPRFDALVAEFKGKAFRPM
                     TKFNRVAFGIVTAIIASNCFQALWSTGVPPFWGQGDPVRFSFNPKYVIWSSDSWEGMW
                     SGIDFLGKRNVKDPDFAYKPNAAKLGIEFKHDAADAPVKLTGALSIANIRPIEGVDAK
                     VNTLSKINGQYLIGSKYSFWALDESLKPVVSAAYDP"
     gene            complement(951..1097)
                     /locus_tag="ACZPB6_08425"
     CDS             complement(951..1097)
                     /locus_tag="ACZPB6_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR1184529.1"
                     /translation="MLTAAMLLLIFKPEKEKLAWRLLIVSWAVVVLMYVGHVSNAILG
                     VLNI"
     gene            complement(1319..2791)
                     /locus_tag="ACZPB6_08430"
     CDS             complement(1319..2791)
                     /locus_tag="ACZPB6_08430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184530.1"
                     /translation="MPEDLKIDRRTLLKTAVSAGAAAAAPAVLAADANAVRWSEVHDV
                     VIAGAGGAGLAAAVMAGGISKDVVVLEKTQDVGGNTRLADAFNAVDPAVQKPLGIEDS
                     PELHARQMLETGRWCADPKLVETVATGAPRALAWLKELGVRFEPGVYQVYGSLWPRTH
                     SPSEPEGSGYIRPLLFKCRELGVSIRTGTKVLRVIRNGLEGPVLGVEAERMNGRRLFI
                     RARRGVVVATGGFGANAELVGRFAPRLGHLRSTNLPGTTGDLIAPLEDIGAAAVGMDF
                     FQLLPGSAEDGRFIGAVSPVERMIFVNRAGERFIAEDSPSSKLSAAVLAQPGGIAFPI
                     LDANGYSAMRTLSRAMFDRALMRGDAFEANSIPELASLLQIPAEALVATVDAYNEAVE
                     TGHDPVGRRAAVLMHRIARRPFYGAKVTMCVNCTLGGIAITPRAEVLDRHGRVIPRLY
                     AAGEVTGGVHGANYMGGNALSDVFTFGRIAGENAVQEPFA"
     gene            2896..>3543
                     /locus_tag="ACZPB6_08435"
     CDS             2896..>3543
                     /locus_tag="ACZPB6_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184531.1"
                     /translation="MISQSRTPAGILRTLTAILCLAAILMSVTGSSAWAAPRVVRVVG
                     IQDGISDSQADYFRRTIAELNDLTEDRIFVTRPIPAASSLDLNAYQGLDFAIVSPRVF
                     ALLERYNGFIPMASLSVDIPDSEPGAELRPVVSALVFATPDADEDGLAKPHLSDIAGE
                     TVTVVKGDEVDTTFLLQNELSSRRLAPVKVRVASDGGTPSEIIRRAVERGDKLFAV"
BASE COUNT          661 a         1064 c         1234 g          584 t
ORIGIN      
        1 cacggatcat aagcggcgga gacgaccggc ttgagggact cgtcaagggc ccagaagctg
       61 tacttggagc cgatgaggta ctggccgttg atcttcgaga gcgtgttgac cttggcgtcc
      121 acgccttcga tcggacggat gttcgcgatc gagagagcgc cggtcagctt gacgggcgcg
      181 tcggcggcgt catgcttgaa ctcgatgccg agcttggcgg cattgggctt gtaggcgaag
      241 tcgggatcct tcacgttgcg cttgccgagg aagtcgatgc cggaccacat gccttcccag
      301 ctgtccgaag accagatcac atacttcggg ttgaaggaga agcggaccgg gtcaccctgg
      361 ccccaaaacg gaggaacgcc ggtggaccag agggcctgga agcagttcga ggcgatgatg
      421 gccgtgacga tgccgaaggc gacgcggttg aacttcgtca tcggacggaa ggccttgccc
      481 ttgaattcgg ccacgagggc gtcgaagcgc ggggcgaggt agacggcgag accgaagagg
      541 aagacgacgc accagaagac gatttcggcc cagaactggg tgtggatgcc gaagacgtca
      601 agaccgaagc cctggccgac gtcacgcatg gcgtggttgc cgtagtggcg gaagctcatg
      661 tagaggccga cggcagccat cacgagcatc gtggcgaggt acttgggctt gaagccatag
      721 cgcaccatga agagcgcgac gacgccgatg taggccattt cctgacgctg gccccagcac
      781 agcgtgcagg gagagtcgcc catgaagtag ccgaggatca cgttggcgat gccgacggga
      841 agaagaacga tgaggagacc gccccagcag atgaggtcgt agaaggtctt ggccttcgag
      901 atgtccttgt cgttgacggg aagagcatag ttcgtcatga tcggctctcc ttagatgttg
      961 aggacgccga ggatggcgtt ggagacgtga ccgacgtaca tgaggacgac gacggcccag
     1021 gacacgatga ggagcctcca cgcaagcttt tccttctcag gcttgaagat gagaagcagc
     1081 atggcggcgg tgagcataag aaattccagg aattccatga tcagcctata aggttgagta
     1141 cttcgagccc gccgtatcag gcggggaacg gaaatgcacc aagaaaacat gtactgcatc
     1201 gttgcataac gcaaacctct gcaacacagt gtttcttaaa tacgcaggcc gtggattata
     1261 tacaatgggc ggggctaggg agaatgccgt agcgtgtatg cggtacgaac tttgtatctc
     1321 acgcaaacgg ctcttgcaca gcgttttcac ccgcaatgcg tccgaacgtg aagacgtcgg
     1381 agagggcgtt gccgcccatg tagtttgcgc cgtggacgcc gcccgtgacc tcgcccgcgg
     1441 cgtagaggcg ggggatgacg cggccgtgtc ggtcgagcac ttcggcccgc ggggtgatgg
     1501 caatgccgcc gagcgtgcag ttgacgcaca tggtgacctt ggcgccgtag aaggggcgtc
     1561 gggcgatgcg gtgcatgagg acggcggccc tgcgaccgac ggggtcgtgc cccgtttcga
     1621 cggcctcgtt gtaggcatcg acggtcgcga cgagggcttc ggcggggatc tgaagcaggg
     1681 aggcgagttc ggggatggag ttcgcctcga aggcgtcgcc gcgcatgagg gcgcggtcga
     1741 acatggcgcg cgagagcgtg cgcatggccg agtagccgtt ggcgtcgaga atggggaagg
     1801 cgatgccgcc gggctgggcg aggacggcgg cggaaagctt cgagctcggg ctgtcctcag
     1861 cgatgaagcg ttcgcccgcg cggttgacga agatcatgcg ctcgacgggg gagacggcgc
     1921 cgatgaagcg gccgtcctcc gcgctgccgg ggagaagctg gaagaagtcc atgccgacgg
     1981 cggccgcgcc gatgtcctcg agcggcgcga tcaggtcgcc cgtggtgccg ggcaggttcg
     2041 tcgagcgaag gtgcccgagc ctcggggcga agcgcccgac gagttcggcg ttcgcgccga
     2101 agccgcccgt ggccaccacg acgccgcgcc tggcgcggat gaagaggcgc cgtccgttca
     2161 tgcgctcggc ctcgacgccg aggacggggc cctcgagccc gttgcggatg acgcgcagca
     2221 ccttcgtgcc cgttctgatc gagacgccga gctcgcggca cttgaagagg agcggacgga
     2281 tgtagcccga gccctcgggt tcggacggac tgtgcgtgcg gggccagagg gagccgtaga
     2341 cctgatagac gccgggctcg aaccgcacgc cgagctcctt gagccaggcg agcgcacggg
     2401 gcgcacccgt ggcgacggtc tcgacgagct tcggatccgc acaccagcgg cccgtctcga
     2461 gcatctggcg ggcgtgcagc tcgggcgagt cctcgatgcc gagcggcttc tggaccgcgg
     2521 ggtcgaccgc attgaaggcg tcggcaagac gcgtgttgcc gcccacgtcc tgggtcttct
     2581 cgaggacgac gacgtccttt gaaatgccgc ccgccatgac ggcggcggca agacctgcgc
     2641 cgcccgcgcc cgcaatcacc acgtcgtgaa cctcgctcca gcggacggcg ttcgcatcgg
     2701 ccgcgagcac ggcgggtgca gcagcggctg cgcccgccga gacggcggtc ttgagcaggg
     2761 ttcggcggtc gatcttgagg tcttcgggca tgtggctcct gtgctgggca agtggaaggg
     2821 acagaaagtt tcggccgcat ggtcccgact ctccgtacaa taggagtccg ggccacaccc
     2881 gaaatgcgtt ccacaatgat aagtcaatcc cgaacacctg caggaatcct ccgcacgctc
     2941 accgccatcc tctgcctggc ggccattctg atgtccgtga cgggctcgtc ggcatgggcg
     3001 gcgcccagag tcgtgcgcgt cgtcggcatc caggacggca tctccgattc gcaggccgac
     3061 tacttcaggc gcacgattgc cgaactcaac gacctcaccg aggaccgcat cttcgtgacg
     3121 agacccattc ccgccgcctc gagccttgac ctcaacgcct atcagggact cgacttcgcg
     3181 atcgtgagcc cgcgcgtctt tgcgctcctc gagcgctaca acggcttcat tccgatggcg
     3241 tcgctgagcg tggacatccc cgacagtgag cccggcgccg agcttcgccc cgtcgtctcc
     3301 gcgctcgtct tcgcgacgcc cgacgccgat gaggacggtc tggcgaagcc gcacctgagc
     3361 gacatcgcgg gcgagaccgt caccgtggtc aagggcgacg aggtcgacac gaccttcctc
     3421 cttcagaacg agctttcgag ccgtcgccta gcgcccgtca aggtgcgtgt cgcctccgat
     3481 ggcgggacgc cctccgagat catcagaagg gcggtcgagc gcggcgacaa gctcttcgcg
     3541 gtc
//
LOCUS       DBMDTI010000206         3531 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6056_length_3531_cov_4.502332, whole genome shotgun sequence.
ACCESSION   DBMDTI010000206 DBMDTI010000000
VERSION     DBMDTI010000206.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6056_length_3531_cov_4.502332"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="ACZPB6_08440"
     CDS             complement(<1..652)
                     /locus_tag="ACZPB6_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184532.1"
                     /translation="MLVPPLSNESRTRNDSPVPERLFELSNLLKRTLSPADHAFALRA
                     ALEAHAMGADESAVVATAAACGLDRAGEALEPLLTLEELAAASRISVGLLNVRAVAHS
                     SDAVADGMLALARVRIRELLDDARLHSGPDDAALEAPDEYVEPFHNILLATAGRPGAA
                     ALRDALHAWVGMLGIEPAWMQEERPAYWMIGLPGDRNLELFADVFRRLGFLFGKMPG"
     gene            complement(778..2034)
                     /locus_tag="ACZPB6_08445"
     CDS             complement(778..2034)
                     /locus_tag="ACZPB6_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024601.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR1184533.1"
                     /translation="MAISTQTRITRARIQLCMRKPYLSTALMRFPLVQADGIIETICT
                     DGYHIFWSESFIDSLTDAELRGVLAHELMHVITQSADRRQERDKHLWNLATDYAINQV
                     LVSCGFRLPGGALLSPLYRDLSAERIYELLEHKGSDNPPPLHSGGSQGQNKQRKDSRG
                     RNKQREDSDECVLKDVGEDVLDPNSPEATRLRTLTGIEMPDEQEVDEAVEATRLETLK
                     ALRSAGITGGSLESLLQGSKISRIDWRTLLAEWMYDRIRDDWSTWPPSKKHICRGLYL
                     PSPGRPAPIKLVMFVDTSGSMSDESLERIFGEIRTFRETFPTPFVIIQADAGIQSVDE
                     YDAYEDFNFEKVKIHGRGGTEFISGYNWIEEHFDSEPVAIIHATDGWGKFPRFCRDPV
                     VFLIPKEVEERSDLRQFPEWGRKIIL"
     gene            complement(2044..3027)
                     /locus_tag="ACZPB6_08450"
     CDS             complement(2044..3027)
                     /locus_tag="ACZPB6_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019346.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1184534.1"
                     /translation="MKASRLTTVLENLLDQPWAAFIWGAPGIGKSSIVRQIAERRRMP
                     LIDIRASLLDPTDLRGIPMIQDGTAVWCPPSFLPKKSDKPGILFLDEINAAPPLVQAA
                     LYQLILDRRVGEYELPEGWRIIAAGNRREDKAVTFRLSSALANRFIHLNLEVDPDDWH
                     AWATNRGIIPEVTAFLKYRPQLLQAASEDEQAFATPRSWEMVSDVVAKFGSPAKARDV
                     IPGIVGTGPAVEFLTFAKKAVLRKEIDAIIADPAGATIPTELDRLWVLVSFLVAGAQN
                     DGILKIVPTLLPRLPVEFSIVLLRDLIRCRPQIVQKKGVSDFLKTNKVMLF"
     gene            complement(3196..>3531)
                     /locus_tag="ACZPB6_08455"
     CDS             complement(3196..>3531)
                     /locus_tag="ACZPB6_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HIR1184535.1"
                     /translation="KRPENLSEKEHTKLAELSKLNTPLLKCYLMGDELDWIHRATHSR
                     IKPLVDFGKNLRGYVQGIIAAADFKINTSVLEGVNNKIKQIKRRAYGFRDDLYFFLKV
                     KAAFHGLPR"
BASE COUNT          612 a          995 c         1224 g          700 t
ORIGIN      
        1 cgcccggcat cttgccgaaa aggaagccga gcctgcggaa gacgtcggcg aagagctcga
       61 ggttgcggtc gcccggcagt ccgatcatcc agtaggcggg gcgttcctcc tgcatccacg
      121 cgggctcgat cccgagcatg ccgacccagg catgaagggc gtccctcagg gcggctgcgc
      181 ccggacggcc ggctgtcgcc agaaggatgt tgtggaacgg ctcgacgtat tcgtcgggcg
      241 cttcgagcgc ggcgtcgtcg gggccgctgt gaaggcgtgc gtcgtccaga agctcgcgga
      301 tgcggacgcg ggcgagtgcc agcattccgt cggccacggc gtcggaggaa tgtgcgacgg
      361 cgcgcacgtt gagaagaccc accgaaatgc gtgaggcggc ggcgagctcc tcaagcgtga
      421 gaagaggttc gagcgcctcg cccgcacggt caaggccgca ggcggcggcc gtggcgacga
      481 cggcggattc gtccgcgccc atggcatgcg cttcaagcgc cgcgcgaagc gcaaacgcgt
      541 ggtctgcggg cgacagcgtc cgcttgagaa ggttggaaag ctcgaagagc cgctcgggaa
      601 cgggcgagtc gtttcgggtc cggctttcgt tggatagagg aggaaccagc atggcgtatt
      661 gacctgactt gggttgatgg gaacgtgtcc tcgggtgcgg tcaggggcgt ccgggcatag
      721 agcgccactc tcgagagctg cgctccgtgc gcttggccct gaaccggtcg agcgtcgtca
      781 gagaatgatt tttcggcccc attcggggaa ctgcctgaga tcggatctct cctccacctc
      841 cttcggaatg aggaagacga cgggatccct gcagaaccgg ggaaacttgc cccagccgtc
      901 ggtggcgtgg atgatggcga cgggttccga gtcgaagtgc tcctcgatcc aattgtagcc
      961 tgagatgaat tcggtgccgc cgcgtccatg gatcttcacc ttttcgaagt tgaagtcctc
     1021 gtaggcgtcg tattcgtcga cgctctgaat gccggcgtcg gcctgaatga tcacgaaggg
     1081 cgtggggaag gtctcgcgga aggttctgat ttcaccgaag atgcgctcaa ggctctcgtc
     1141 ggacatcgaa cccgaggtat cgacgaacat cacgagcttg atcggtgcgg gcctgcccgg
     1201 gctgggaagg tagaggccgc ggcagatgtg ctttttcgaa ggcggccacg tgctccagtc
     1261 gtcgcggatg cggtcgtaca tccactccgc gagaagcgtt cgccagtcga tgcgggagat
     1321 cttggagccc tgcagaagac tctcgaggct gccgcccgtg atgcccgccg acctgagcgc
     1381 cttgagggtt tcgagacggg tcgcctcgac ggcctcgtcg acctcctgct cgtcgggcat
     1441 ctcgatgccc gtgagcgtcc tcaggcgggt tgcctcgggg ctgttcggat cgaggacgtc
     1501 ttctccgacg tccttcagaa cgcattcatc ggaatcctcc cgctgcttgt tccggccccg
     1561 ggaatccttc cgctgcttgt tctggccttg ggagccgccc gaatgcagag gcggcggatt
     1621 gtcgcttccc ttgtgctcga ggagctcgta gatccgttct gcgctcaagt cccggtagag
     1681 cggggagagg agggcacccc cgggaagcct gaagccgcag ctgacgagca cctggttgat
     1741 ggcgtagtcg gtcgcaaggt tccagaggtg cttgtcgcgt tcctgcctgc ggtcggcgct
     1801 ctgcgtgatg acgtgcatga gctcgtgcgc gagcacgccc ctgagttcgg cgtcggtgag
     1861 cgagtcgatg aagctttcgc tccagaagat gtggtagccg tcagtgcaga tggtctcaat
     1921 gatgccgtcc gcctgaacga gcgggaagcg catcagtgcc gtcgacagat agggctttcg
     1981 catgcagagc tgaatgcgcg cgcgtgtgat gcgcgtctgc gtcgagatgg ccatgcgccc
     2041 tccttagaac agcatgacct tgttggtctt gaggaagtcg ctgacgccct ttttctgcac
     2101 gatctggggc ctgcaccgga tcaggtcgcg cagaagcacg atgctgaact ccacgggcag
     2161 gcggggcagc agcgtgggca cgatcttgag gatgccgtcg ttttgggcgc ctgccacgag
     2221 gaaggacacg agcacccaca ggcggtcaag ctcggtgggg atcgtcgcgc cggcggggtc
     2281 ggcgatgatg gcgtcgattt ccttgcgcag cacggctttc tttgcaaagg tcaggaactc
     2341 gacggcgggg ccggtcccga cgatgccggg gatcacgtcg cgggctttgg cagggctgcc
     2401 gaacttcgcc accacgtcgc tcaccatctc ccagctgcgg ggcgttgcaa aagcctgctc
     2461 gtcctcggac gcagcctgca gcagctgcgg gcggtacttg aggaaggcgg tgacttcggg
     2521 gatgatgccg cggttggtgg cccaggcgtg ccagtcatcg gggtcgacct caagattgag
     2581 gtggatgaag cggttggcga gcgcgctcga gagcctgaag gtcaccgcct tgtcctcgcg
     2641 gcgattgcct gccgcaatga tgcgccagcc ttcgggaagt tcgtattcgc cgaccctgcg
     2701 gtcgagaatg agctgataga gcgcggcctg cacgagagga ggcgcggcgt tgatctcgtc
     2761 gaggaagaga atgccgggct tgtcggactt cttcggcagg aaggacggcg ggcaccagac
     2821 ggccgtgccg tcctggatca tcggaatgcc gcgcaggtcg gtcggatcga gcagggaggc
     2881 gcggatgtcg atgagcggca tgcggcggcg ctcggcgatc tggcgcacga tcgagctctt
     2941 gccgatgccg ggggcgcccc agatgaaagc ggcccagggc tggtcgagaa ggttttcaag
     3001 tacggtggta agtcgcgagg ctttcatgtt ggtggttcgt tggcttccgt tgagaggtgt
     3061 gggtgccgtc ctgcaggaca tgcaggaagc ggtgccatac aggaggcatt ctattggagg
     3121 tgcggaaaca acgcgtccct cgttgtccgg ccggtcggcc ttgtgcatgc gcgcggcgga
     3181 ggaagaaaaa aaggttcatc tcggaagtcc gtggaacgcg gccttgactt ttaagaaaaa
     3241 gtacagatcg tctcggaagc cataagctcg tcgcttaatt tgcttgattt tattgttgac
     3301 accttcaagt acagaggtgt tgatcttaaa gtccgcggct gcaatgatgc cctgcacata
     3361 gcccctgagg ttcttgccga aatctaccaa gggcttgatc ctgctgtgag tggctcggtg
     3421 aatccagtcc agctcgtctc ccatgagata gcatttgagc aacggcgtat taagtttcga
     3481 aagctccgcc agttttgtat gctccttctc cgagagattt tcagggcgct t
//
LOCUS       DBMDTI010000207         3529 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6061_length_3529_cov_3.144107, whole genome shotgun sequence.
ACCESSION   DBMDTI010000207 DBMDTI010000000
VERSION     DBMDTI010000207.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6061_length_3529_cov_3.144107"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="ACZPB6_08460"
     CDS             complement(<1..1369)
                     /locus_tag="ACZPB6_08460"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR1184536.1"
                     /translation="MAEVKTTCREELLAKLREDPVWDVVVIGGGATGAGIAVDAASRG
                     LKTVVLEAQDFSSGTSSRSTKLIHGGVRYMKNPRDWGLVREALKERRILIKNAPHLVH
                     SKPFILPCYKKGEREFYTIGLGIYSAMTYGGYNIGHTSSLSREDTLMRLPGVKPEGLM
                     GGVQFFDGQFDDARLDVALIRTAYEHGAVPLNYMPVVGVTREGGMIRSVTAKDKETGE
                     EFVIRTKMVFNAAGAWVDPIRRMVDPDASTLVRVSRGSHILLDKSFMPGDTGMLIPKT
                     RDGRVLFAIPWHGMLLVGTTDVEQKEADFDPKVSDAEIDFMIETASGYLEKPITRADV
                     KASFAGLRPLFNPQATGSAGSTAKVSREHAVIPEFGNMITVTGGKWTAYRKMAEHAML
                     VATLRHLIPPRLCVTKDLPIFCDPNWDHAALEIEAEAGDAADEKVVQFALYARDHEFA
                     RTPEDV"
     gene            complement(1372..2862)
                     /gene="glpK"
                     /locus_tag="ACZPB6_08465"
     CDS             complement(1372..2862)
                     /gene="glpK"
                     /locus_tag="ACZPB6_08465"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR1184537.1"
                     /translation="MKYILALDQGTTSSRAILLNHRAQIVSLEQLEFPQHYPQPGWVE
                     HDAMDIWRTQLACAQNCLRKAGVSASEVSAIGVTNQRETTVVWDRTTGLPIAPAIVWQ
                     DRRTAPYCTELQRRGFEVLVREKTGLRIDPYFSATKIRWILDRVPGARARAERGELAF
                     GTIDTWLIWKLTGGRVFVTDITNASRTMLCNLETCRWDDELLDIFGIPASMLPEIVPS
                     SCVVGEALPEMLGAAIPVAGIAGDQQAATFGQTCFEAGCAKNTYGTGGFLLMNVGSEP
                     RMSQHRLIGTAAWQLCCERTQYALEGSVFIAGAVVQWLRDGLQFFNSAEEIESLAKSV
                     PDNGGVYLVPAFVGLGAPHWDSEARGMMIGLTRGTTKAHVARAALEGIAFQCAEVLEA
                     MQLDAGTELKELRVDGGASRNNLLMQFQADISGVPVIRPAITETTALGAAFLAGLSTG
                     VWESCEEISALWRVDRVFEPSMTADRREYLMNQWARAVGRARNWNK"
     gene            complement(3074..>3529)
                     /locus_tag="ACZPB6_08470"
     CDS             complement(3074..>3529)
                     /locus_tag="ACZPB6_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184538.1"
                     /translation="ERRPFVAGERREFNGERRGFGDRRKSFGGGQRRREDFGVRSGPR
                     ARALETRRFSERSDFAKNAVVKLDADIADYFKSAEDVNKALRLVIELGRLAKKPEVKT
                     EAEKVEQAEAPAVAAEEAMFTEDVFEDEDVEAVAEAEETAEAKSEDKAE"
BASE COUNT          625 a         1085 c         1182 g          637 t
ORIGIN      
        1 gcacgtcctc gggcgtgcgg gcgaactcgt ggtcgcgggc gtagagggcg aactgaacaa
       61 ccttttcgtc ggcggcgtca ccggcttcgg cctcgatctc aagagcagca tggtcccagt
      121 tgggatcgca gaagatcggc agatccttcg tgacgcagag acgcggcggg atgaggtgac
      181 gcagcgtggc gacgagcatg gcgtgctcgg ccatcttgcg gtaggccgtc cacttgccgc
      241 ccgtcacggt gatcatgttg ccgaattcgg gaatgacggc gtgttcgcga gaaaccttgg
      301 cggtcgagcc ggcggaaccc gtggcctgcg gattgaagag cggacgcagg ccagcgaagg
      361 aagccttgac gtcggcgcgg gtgatcggct tttcaagata gccggaggcc gtctcgatca
      421 tgaaatcgat ttcggcgtcg ctcaccttcg gatcgaagtc ggcttccttc tgttccacgt
      481 cggtcgtgcc gacgagaagc atgccgtgcc agggaatggc gaagagcacg cggccgtcgc
      541 gggtcttcgg gatgagcatg ccggtgtcgc cgggcatgaa ggacttgtcg agcaggatgt
      601 gggagccgcg ggagacgcgc acgagcgtgg aggcatccgg atcaaccatg cggcggatcg
      661 gatcgaccca ggcgccggcg gcgttgaaga ccatcttcgt gcggatgacg aactcttcgc
      721 cggtttcctt gtccttcgcg gtcacggagc ggatcatgcc gccttcacgg gtgacgccaa
      781 cgacgggcat gtagttgagc ggtacggcgc cgtgttcgta ggccgtgcga atgagcgcaa
      841 cgtcaagacg cgcgtcgtcg aactggccgt cgaagaactg cacgcccccc atcaggcctt
      901 cgggcttgac gccgggcagg cgcatgaggg tgtcctcacg ggagaggctc gacgtgtggc
      961 cgatgttgta gccgccgtag gtcatggcgg agtagatgcc gaggccgatc gtgtagaact
     1021 cgcgctcgcc cttcttgtag cagggcagga tgaagggctt ggagtgcacg aggtgcggag
     1081 cattcttgat gaggatgcgg cgttccttca gagcttcgcg cacgagcccc cagtcacgcg
     1141 gattcttcat gtagcgcacg ccgccgtgaa tcagcttcgt cgaacgggac gacgtgccgg
     1201 acgaaaagtc ctgggcctcc agcacgacgg tcttgaggcc gcgggaggcg gcgtcgaccg
     1261 caatgccggc gcccgtggcg ccgccgccga tgacgacgac gtcccagacg ggatcttcac
     1321 gaagtttggc gagcagttcc tcgcggcagg tggttttcac ttcagccatg gtcacttatt
     1381 ccagttgcgg gcgcggccga cggctcgggc ccattggttc atcaaatact ctcgcctgtc
     1441 ggcggtcatc gagggctcga acaccctgtc gacgcgccac aaggcggaaa tttcttcaca
     1501 cgactcccag acgcccgtcg aaaggccggc aaggaacgcc gccccgagcg cagtcgtttc
     1561 ggtaatggcg ggacgaatga ccggcacgcc ggagatgtcc gcctgaaact gcatgagaag
     1621 gttgttgcgg gaggcgccac cgtcaacgcg cagctccttg agctcggtgc ctgcatcaag
     1681 ttgcatcgcc tcaagcacct ccgcacactg gaaggcgatg ccctcaagcg cggcgcgcgc
     1741 gacgtgcgcc ttcgtcgtac ctctcgtcag cccgatcatc atgccgcggg cctcgctgtc
     1801 ccagtgcggg gcgccgagcc ccacgaaggc gggaacgaga tagacgccgc cgttgtcggg
     1861 cacgctcttg gcgagcgact cgatctcctc ggcggaattg aagaactgca ggccgtccct
     1921 gagccactgc acgacggcgc cggcaatgaa gacggagccc tcaagcgcat actgcgtgcg
     1981 ttcgcagcag agctgccagg cggccgtgcc gatcaggcgg tgctggctca tgcggggctc
     2041 gcttcccacg ttcatcagaa ggaagccgcc cgtgccgtag gtgttctttg cgcagccggc
     2101 ctcaaagcac gtctgaccga aggtcgcggc ctgctggtca cccgcaatgc ccgccacggg
     2161 aatcgcggcg ccgagcattt cgggtagcgc ctcgccgacc acgcatgagc tgggcacgat
     2221 ttcgggaagc atcgaggcgg gaatgccgaa gatgtcgaga agttcgtcat cccacctgca
     2281 ggtttcgaga ttgcagagca tcgtgcggga ggcgttcgtg atgtcggtca cgaagacgcg
     2341 gccgcccgtg agcttccaga tgagccacgt gtcgatcgtg ccgaaggcga gttcgccgcg
     2401 ttctgcgcgc gcacgcgctc cgggcaccct gtcgagaatc cagcggatct tggtggcgga
     2461 gaagtacgga tcgatgcgaa ggcccgtctt ttcacgcacg agcacttcaa agccccttct
     2521 ctgcagctcc gtgcagtagg gagccgtacg gcggtcctgc cagacgatgg caggggcaat
     2581 cggcagcccc gtcgtcctgt cccagacgac cgtcgtctcg cgctggttcg tcacgccgat
     2641 ggcgctgacc tcggaggccg agacgcccgc ctttctcagg cagttctggg cgcaggcgag
     2701 ctgcgtgcgc cagatgtcca tggcgtcgtg ctcgacccag ccgggctgcg gataatgctg
     2761 cgggaactca agctgctcca gggaaacgat ctgggcgcgg tgattcagca gaatggcgcg
     2821 ggaactcgtg gtcccctgat caagagcaag aatgtatttc atgtaagcgg ccggtctcgc
     2881 agccccggcg cacgcacggg ggtggttttc ggattttgag ccttgaaagt ttaactaaaa
     2941 aggccgtgcc tgcctagggg agattcccga tggcagggga gtggaccacc accatccgcc
     3001 agagacgaaa aaagctccgc tttccctgcg ggagcgaagc tttcaagtga cctgcgtcac
     3061 ccgtcagaga gccttattcg gccttgtcct cggacttggc ttcagccgtt tcctcagctt
     3121 cggcgacagc ctccacgtcc tcgtcctcga agacgtcttc cgtgaacatg gcttcttcgg
     3181 cggcgacggc gggagcctcg gcctgctcga ccttttcagc ttccgtcttg acctcgggct
     3241 tcttggcaag cctgccgagc tcgatcacca ggcggagggc cttgttgacg tcctcggcgc
     3301 tcttgaagta gtcggcgatg tcggcatcaa gtttcacgac ggcgttcttg gcgaagtcgg
     3361 agcgctcgga gaagcggcgg gtttcaagcg cgcgggcacg cggaccggaa cgaacgccga
     3421 agtcttcgcg acgacgctgg ccgccgccga aggacttgcg acgatcgccg aagccacggc
     3481 gctcaccgtt gaactcacga cgctcgccgg ccacgaacgg acgacgttc
//
LOCUS       DBMDTI010000208         3524 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6075_length_3524_cov_3.342974, whole genome shotgun sequence.
ACCESSION   DBMDTI010000208 DBMDTI010000000
VERSION     DBMDTI010000208.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6075_length_3524_cov_3.342974"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="ACZPB6_08475"
     CDS             complement(<1..285)
                     /locus_tag="ACZPB6_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR1184539.1"
                     /translation="MEKQSKKDLNIMYKRILVPTDGTPMSEKAVEGAARFAKSLGASL
                     VLITVVEPYSYTNLSEYRPESIEQYDERVTAEAKDRLADAKRRCDEIGVPS"
     gene            complement(334..1122)
                     /locus_tag="ACZPB6_08480"
     CDS             complement(334..1122)
                     /locus_tag="ACZPB6_08480"
                     /EC_number="6.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_function: GO:0061503 - tRNA threonylcarbamoyladenosine
                     dehydratase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HIR1184540.1"
                     /translation="MSVTTDPRRFGGVGRLFGEEGARRLAGAHVAVVGLGGVGSWVVE
                     ALVRTSVGRITLIDGDDVALSNTNRQLPAFEGAYGRAKAEVLRERCLLINPDCRIEVK
                     TVFVTPENAVQVIPSDCDWLVDCIDDLKGKAALHAAARGMGVRTVCAGGAGARIDPSR
                     IEVSDLARVKGDPLLGKLRTFLRKEYGFPAGSADGRSTLFGIPAVYSTEPLRQPGADS
                     LEAIGAEPGSRIGFGSFVSVTGSAGLRLAGIVINDIAGAQNTAD"
     gene            1374..2273
                     /locus_tag="ACZPB6_08485"
     CDS             1374..2273
                     /locus_tag="ACZPB6_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR1184541.1"
                     /translation="MRILVPVDGSENSMHAVEFIASRTTLLGSNPEIEVLNVQLPLPA
                     RACRLVGQDSLTRYYEDEAAKVFEPVRAELNKVGFQAGEAFLVGEASDSIAAEAEKFG
                     ADLIVMGSRGQTALRGLFFGSVSNGVLAKSKCPVLMLRDKAAPQSDALKVGIAVDGSK
                     YGRAAVRYALKHISLFGTGAQFYLINVVSDYAGAVMPDMAGMALPALSEEEVLELQKD
                     EFNEAVEPLRPLFAKAAVATKEICLVGNAGDEIAAFAKKKKLDLVVMGSHGYGRFKAA
                     VMGSTATRIAATGDVPLLIIRQA"
     gene            2514..3377
                     /locus_tag="ACZPB6_08490"
     CDS             2514..3377
                     /locus_tag="ACZPB6_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012422.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR1184542.1"
                     /translation="MITLTEEQLSSSGTTAAGKDSKKSPASSGRRLDTSDLQEGRGGR
                     RYVNGTFPREQDRDSKFSSSAPSSRIRVLIAANSKTEARSLSAMLSDRNEFMVVGTAT
                     TFNELDATLHLRTDVDVLVTDVELGGATVFPIVEKAVTLRPNLNVLCYTNRSEPNFIM
                     NAIRAGALGYVLRNSQESLDNCVRLINGGGSPMSPAVTRHVLRAMQMKKNSEPLVAAT
                     NDAPDISTREREILELLAKGIPFAEIGTILMISPHTVTAHIKKIYRKLQVHSRGEAVY
                     EARALNLISDS"
BASE COUNT          663 a         1224 c         1021 g          616 t
ORIGIN      
        1 cgagggtacc ccgatttcat cgcagcggcg cttggcgtcg gcaagacggt ccttggcctc
       61 ggcggtcacg cgctcgtcgt actgctcgat cgattccgga cggtattcgg aaagattcgt
      121 gtaggagtac ggctcgacga cggtgatgag cacgagcgaa gcgccgagag acttggcaaa
      181 gcgggcggcg ccctccacgg ccttttccga catcggcgtg ccgtcggtcg gaaccagaat
      241 gcgcttgtac atgatgttga gatccttttt actctgtttt tccatggcgc cgtcctgcgc
      301 gggacgacgc atatcttttc attgtaggcg gattcagtct gccgtgtttt gagcgcccgc
      361 aatatcgttg atcacgattc ccgccagcct gaggcctgcg gaacccgtca cggagacgaa
      421 ggagccgaag ccgatccggc tgccgggctc ggcgccgatc gcctccagac tgtcggcgcc
      481 gggctgcctg agaggctccg tgctgtagac cgcgggaatg ccgaagagcg tcgagcggcc
      541 gtcggccgaa cctgcgggga agccgtactc ctttctaaga aaggtgcgta gcttcccgag
      601 aagcggatca cccttgacgc gcgcgaggtc cgaaacctcg atgcgcgagg gatcgatgcg
      661 cgcgcctgcg ccgcccgcgc agacggtcct cacgcccatg ccgcgggccg ccgcatgcaa
      721 cgcggccttt cccttgaggt cgtcgatgca gtcgacgagc cagtcgcagt cggacggaat
      781 gacctgcacc gcgttctcgg gcgtcacgaa gaccgtcttc acctcgatgc ggcagtcagg
      841 attgatgagc aggcagcgct ccctgagcac ctcggccttc gcgcgtccgt aggcgccctc
      901 aaaggcgggc agctggcggt tcgtgttcga gagcgccaca tcgtcgccgt cgatcagcgt
      961 gatgcggccc accgacgtgc ggaccagagc ctccacgacc cagctgccga cgccgcccag
     1021 ccccaccacg gccacatgcg cgccggcgag gcgcctggcg ccctcctcgc cgaaaagacg
     1081 gcccacgcct ccgaatcgcc tcgggtcagt cgtcacgctc atatcagttc tccatttgtc
     1141 cgaacaatta gatttttaaa atgtgagtga agttttccga gaggctcaat ttcgtttttt
     1201 attgctttag agcaagaaaa cgacggtgcg gacgtcttgg ctttgatgcc gccgcagtcg
     1261 tttcagagta cattgtcctc aaggacggaa gttcttcgcc accgcgagtg taaagcacgg
     1321 cgacggcaaa gaacaacgtg accaccgtcc tgcaaagatc ctcaggaggt tatatgcgca
     1381 ttctcgttcc cgttgacggc agtgaaaaca gcatgcacgc cgtggaattc atcgccagcc
     1441 gcaccacgct tctcgggagc aatccggaaa tcgaagtcct gaacgttcag cttccgcttc
     1501 cggcacgcgc ctgccgactc gtcggccagg actccctgac ccgctactat gaagacgaag
     1561 ccgccaaggt cttcgaaccc gtgcgcgccg agctcaacaa ggtcggcttc caggcaggcg
     1621 aggccttcct cgtcggcgaa gcctccgaca gcatcgcggc cgaagccgaa aagttcggcg
     1681 ccgacctgat cgtcatgggc tcccgcggcc agaccgctct tcgcggcctc ttcttcggct
     1741 ccgtctcgaa cggcgtcctc gccaagtcca agtgccccgt cctgatgctg cgcgacaagg
     1801 ccgcgccgca gtccgacgct ctcaaggtcg gcatcgcggt cgacggctcc aagtacggcc
     1861 gcgccgccgt gcgctacgcc ctcaagcaca tctcgctctt cggcacgggc gcacagttct
     1921 acctcatcaa cgtggtgagc gactacgccg gcgccgtgat gcccgacatg gccggcatgg
     1981 ccctgcccgc actctccgaa gaggaagtgc tcgaactgca gaaggacgag ttcaatgaag
     2041 ccgttgagcc gctgcgtccg ctctttgcca aggccgccgt cgcgaccaag gaaatctgcc
     2101 tcgtcggcaa cgcaggcgac gaaatcgccg ccttcgcgaa gaagaagaag ctcgacctcg
     2161 tggtcatggg ctcccacggc tacggccgct tcaaggccgc cgtcatgggc tcgaccgcaa
     2221 cccgcatcgc cgccacgggc gacgttccgc ttttgatcat ccgtcaggcc tgacggcctg
     2281 agcgcagcgg gcggttcctg caggatgccg cccgccgctg aagatagccg gcctctggcc
     2341 ggctatcttt ttccaccggc atacgccctg gagcacctgc aacacaggca tttcgacgaa
     2401 cagcacgcct gaccatcccc gatttgcggg atgtcgtaga atacctgcat gttccgccgg
     2461 gcagcgtctc agccgcccgg cgccccacct ctgcgaccct gacaaatcca aagatgatta
     2521 ctctcaccga agaacagttg agttctagcg gcacgaccgc cgcgggcaag gactccaaga
     2581 agtcgcccgc ttcctccggc cgcaggctcg acacctccga cctccaggag ggcaggggcg
     2641 gccgccgtta tgtcaacggc acgttccccc gcgaacagga ccgcgactcc aagttttcga
     2701 gctccgctcc ctcaagtcgc atccgcgtgc tgatcgccgc gaacagcaag acggaggccc
     2761 gctccctgag cgccatgctc tcggaccgca acgagttcat ggtcgtcggc accgccacga
     2821 ccttcaacga gcttgacgcc acgctgcacc tgcgcaccga cgtcgacgtg ctcgtcaccg
     2881 acgtcgaact cggcggcgcc acggtcttcc ccatcgtcga aaaggctgtc acgctccgcc
     2941 cgaacctcaa cgtgctctgc tacacgaacc gcagcgagcc gaacttcatc atgaacgcca
     3001 tccgtgcggg tgcgctcggc tacgtgctac gcaactccca ggaaagcctg gacaactgcg
     3061 tgcgcctcat caacggcggc ggatccccga tgtcccccgc cgtgacccgc cacgtcctgc
     3121 gcgccatgca gatgaagaag aactccgagc cgctcgtggc cgccacgaac gacgcgcccg
     3181 acatctccac ccgcgagcgc gagattctcg agcttctggc caagggcatt ccgttcgccg
     3241 aaatcggcac gatcctcatg atctcgccgc acaccgtgac tgcgcacatc aagaagatct
     3301 accgcaagct tcaggtccac tcccgcggcg aagcggtcta cgaggccaga gccctcaacc
     3361 tgatcagcga cagctgacga cgggctccat acgaaaggag gccggcgtgc caaatgcacg
     3421 ccgacctcct ttcgtttcag tcagggaaaa agccgcctca ggccctttca gccgtccagc
     3481 ggcccgactt accgccgtcc ttctcgagaa ggcgtatgtc ggtc
//
LOCUS       DBMDTI010000209         3497 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6137_length_3497_cov_2.021496, whole genome shotgun sequence.
ACCESSION   DBMDTI010000209 DBMDTI010000000
VERSION     DBMDTI010000209.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6137_length_3497_cov_2.021496"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="ACZPB6_08495"
     CDS             <1..1415
                     /locus_tag="ACZPB6_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR1184543.1"
                     /translation="VMCLSLIGISADYTTYYLMRRRWAGGIETPAGTLVHLRPSLLHA
                     LLTTVTAYALMLAAPFPGLRQLALFAVCGLAGAWLTVVAWFPFLVKGFPVRPLPARSL
                     LSAYADAWTQGRLWTKAFLVLFACVSVFGVARLSVNDDLSALQTPPVRLAAEEKVISS
                     LLGIGFSQTWFAATGKTPDEAVQTLELLRPKIHELEKEGFISRPVMVPLRSLDVQEKA
                     LASMKAAEPSMTAKLAPPCEGSGCTPYGTVKLASWLAGPLGDNYRSLVARTSEGVVIL
                     VPVTLSAGKDAAEAAAAAAAGIDGVYWLNRRADFGELFSAFRLGLTGLIAAGLAVLGL
                     MLVKLFGARLGLVASLPVAGGLLAGLAAAGITGTPVNLFSLFALILVMGIGVDYTIFF
                     HSEEGAGSADEPRDHVLHAMAVALGSTLLSLGILVLSETPAVRSFGLVLSAGVLFAFL
                     LAPATRLITRRNTYREERPR"
     gene            1412..2044
                     /locus_tag="ACZPB6_08500"
     CDS             1412..2044
                     /locus_tag="ACZPB6_08500"
                     /inference="COORDINATES: protein motif:HMM:NF023089.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HIR1184544.1"
                     /translation="MTSASFRGLAALAAALMLTGCATEPESPELFFWVKPGVRAALPD
                     SPCGADMTENAILTAVRDGRTERLLTVRACRGGLMSLDVMTLTGMALMSVTYDGTMLV
                     STNYVPLPKELRAEQIVADILMGTLPAEAWTTLPSGYHLRIEEKSRTLVDGDGAEIER
                     FTYADGPRGPRLASLRHEAFGYSIAFRYLEDPAVPDSTADKPGPDEEQAQ"
     gene            2041..3204
                     /locus_tag="ACZPB6_08505"
     CDS             2041..3204
                     /locus_tag="ACZPB6_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012337.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HIR1184545.1"
                     /translation="MTVYVEALSLVNALGEDDDAILANLAEGRAPGMEGPLVRTVDGF
                     EAPFGHVTIDLPDTNPPSSRNNRLLRACWNLRKTEFESRLKDFEDERIAVVLGTSTSG
                     SQEFSDWWKARKFGSEMPPFAGEWQEMGDPSRDFASFVGAKGPAYTIATACTSSARAI
                     ISAARLLEAGLADAVIAGGADTFADMPLNGFHALGVLAGERTRPLTDHRPGITIGEGA
                     GLMLFTRRPSAVKLSGWGESSDGHHMSSPEPEGRGAEAAVRGALSRAGLTPDDIVYVN
                     LHGTGTGQNDASELAAMNRVFGGRTAMSSTKTYTGHTLGAAGVTDAGLLVLGLMHGGL
                     KLPAQFSEGQTPDETLPLSGVLREPALIAPGPVMSTNLAFGGSNTALIFEPNS"
     gene            3218..>3497
                     /locus_tag="ACZPB6_08510"
     CDS             3218..>3497
                     /locus_tag="ACZPB6_08510"
                     /inference="COORDINATES: protein motif:HMM:NF046932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApeP family dehydratase"
                     /protein_id="HIR1184546.1"
                     /translation="MSETTQTEFLDPESYLPHRKPMCVITRVVSVGEADVTTEADLDA
                     DGVFATHWRPDGELPEAFFLEVMAQTIGIWSHVHDDLTVVPVGDRKADA"
BASE COUNT          548 a         1233 c         1086 g          630 t
ORIGIN      
        1 tcgtcatgtg cctgagcctc atcggcatct cggccgacta cacgacctac tatctgatgc
       61 gccgccgctg ggcgggcggg atcgaaacgc ccgcaggcac gcttgtgcac ctgaggccct
      121 cgctcctgca cgcgctcctc accacggtca cggcctatgc gctcatgctc gccgcgcctt
      181 tccccggcct caggcagctc gcgctctttg cggtctgcgg actcgcgggc gcctggctca
      241 cggtcgtcgc ctggttcccg ttcctcgtca aaggcttccc cgtccgtcct ctgcccgccc
      301 gaagcctgct ttcggcctac gcggacgcat ggacgcaagg gcgcctctgg acgaaggcct
      361 tcctcgtcct ctttgcctgc gtatcggtct ttggcgtcgc gcgccttagc gtcaacgacg
      421 acctctcggc cctgcagacg ccgcccgtca gacttgccgc agaagagaag gtgatctcct
      481 cgcttctcgg catcggcttt tcccagacgt ggttcgccgc cacgggcaaa acccccgacg
      541 aggcggtgca aacgcttgag ctcttgcgac cgaagattca tgaactggaa aaagagggct
      601 ttatttcgcg tcccgtgatg gtgcccctca gaagccttga cgtgcaggaa aaggcccttg
      661 cctcgatgaa ggctgctgag ccctcgatga cggcaaagct cgcgccgccc tgtgagggat
      721 ccggctgcac gccctacggg accgtgaagc ttgcgtcatg gctggcgggc ccgttgggcg
      781 acaactaccg ctcgctcgtt gcccgcacgt ccgagggcgt cgtgatcctc gtgcccgtga
      841 cgctctcggc cggaaaggat gcggccgagg ctgccgctgc agctgcggcc gggatcgacg
      901 gcgtttactg gctcaaccgc cgcgccgact tcggggaact cttctcggcc ttccgcctcg
      961 gtctcacggg gctcatcgcc gcaggactcg ccgtgctcgg actcatgctc gtgaagctct
     1021 ttggcgcaag gcttgggctc gtcgcgtcgc tgcccgtcgc gggcggcctg cttgcgggcc
     1081 ttgccgctgc gggcatcacg ggcacgcccg tcaatctctt cagtctcttc gcgctcatcc
     1141 tcgtcatggg gatcggcgtc gactacacga tcttctttca ttcggaggaa ggcgcgggat
     1201 ccgcggacga gccgcgggac cacgtccttc atgcgatggc ggtggcgctt ggaagcacgc
     1261 ttctttcgct cggcattctg gtgctgagcg agacgccggc cgtgcgaagc ttcggcctgg
     1321 tgctttcggc gggcgttctt ttcgccttcc tgcttgcgcc cgccacgcgc ctcatcacaa
     1381 gacggaacac atacagggag gagcgtccac gatgacgtcc gcttctttca ggggccttgc
     1441 ggcgctggcc gccgcgctca tgctcacggg ctgcgccacg gagcccgagt cgcctgaact
     1501 cttcttctgg gtcaagcccg gcgtgcgcgc cgcgctgccc gacagtccct gcggcgccga
     1561 catgaccgaa aacgccattc tcacggcggt ccgcgacggg cgcaccgagc gccttctcac
     1621 ggtcagggcc tgccgcggcg gcctgatgtc gctcgacgtg atgaccctca cgggcatggc
     1681 cctcatgagc gtcacctacg acggcaccat gctcgtgtcg acgaactacg tgccgctccc
     1741 caaggagctt cgcgcagagc agatcgtcgc cgacattctg atgggcacgc tccccgccga
     1801 agcgtggacg acgcttcctt cgggttatca tctgaggatt gaggaaaaat cgagaacgct
     1861 cgtcgacggc gacggcgccg aaatcgagcg cttcacgtat gccgacgggc cccgcggccc
     1921 caggctcgcg agcctcaggc acgaggcctt cgggtattcg atcgcctttc gctatctcga
     1981 ggatcccgcc gttcccgatt ccaccgccga caagcccggc cccgacgagg agcaagccca
     2041 gtgacggttt atgttgaagc cctgtcgctc gtcaacgcac tcggcgaaga cgacgacgcc
     2101 attcttgcca atctggccga aggccgcgcg cccggcatgg agggacccct cgtgcgcacc
     2161 gtcgacgggt tcgaggctcc gttcggccac gtgacgattg atctgcccga cacgaatccg
     2221 ccgtcctcgc gcaacaacag gctcctcagg gcctgttgga acctcaggaa gaccgagttc
     2281 gaaagccgcc tcaaggactt tgaggacgag cgcatcgccg tcgtgctcgg cacgagcacc
     2341 tcgggctctc aggagttctc cgactggtgg aaggcacgaa agttcggctc ggaaatgccg
     2401 ccttttgcag gcgaatggca ggaaatgggc gatccttcgc gcgatttcgc atcatttgta
     2461 ggtgccaagg gccccgccta tacgatcgcg acggcctgca cgagctcggc ccgcgccatt
     2521 atcagcgcag cccgccttct tgaggcgggt ctggccgacg ccgtcattgc gggcggcgcc
     2581 gacaccttcg ccgacatgcc cctgaacggc tttcacgcct tgggcgtgct cgcgggcgag
     2641 cgcacgcgtc ccctgaccga ccatcgtccc ggcatcacga tcggcgaggg tgcaggcctg
     2701 atgcttttca cccgccgtcc ctcggccgtg aagctttcgg gctggggcga gagctccgac
     2761 ggtcaccaca tgtcgagtcc cgaacccgag ggcaggggcg ccgaggcggc cgtgcgcggc
     2821 gcgctctcgc gtgcgggcct cacgcccgac gacatcgtct acgtgaatct tcacggcacg
     2881 ggcacggggc agaacgacgc ctccgagctt gccgccatga accgcgtctt cggcgggcgc
     2941 accgcaatgt cgtccaccaa gacctatacg ggccacacgc tcggcgccgc aggcgtcacc
     3001 gacgcggggc tgctcgtgct cggactgatg cacggggggc tgaagctccc cgcccaattc
     3061 tccgaaggcc agacgcccga cgagacgctt ccgctctcgg gcgtgttgcg cgaacccgcg
     3121 ctcattgcgc ccggccccgt catgagcacg aacctcgcct tcggcggcag caacacggcc
     3181 ctcatcttcg aaccgaattc ctgaacggca cgaatccatg tctgaaacca cccaaaccga
     3241 atttcttgat ccggaaagct atctcccgca caggaagccc atgtgcgtca tcacccgcgt
     3301 cgtcagcgtg ggcgaggcgg acgtcacgac cgaggcggat ctggacgccg acggcgtctt
     3361 cgccactcac tggcgtcccg acggagagct gcccgaggcc ttcttcctcg aggtgatggc
     3421 ccagacgatc ggcatctggt cgcacgtgca cgacgatctt accgtcgtgc ccgtgggcga
     3481 caggaaggcc gacgcgg
//
LOCUS       DBMDTI010000210         3472 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6183_length_3472_cov_2.275883, whole genome shotgun sequence.
ACCESSION   DBMDTI010000210 DBMDTI010000000
VERSION     DBMDTI010000210.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6183_length_3472_cov_2.275883"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="ACZPB6_08515"
     CDS             complement(<1..603)
                     /locus_tag="ACZPB6_08515"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1184547.1"
                     /translation="MQNFEYYNPTRLIFGRGEIASLTRHIPEGVRVLVTFGGGSVKRN
                     GVYDQVKAALAGIDHVEFWGIEANPKVETLRKCIEFGREQKAGFILAVGGGSVLDGSK
                     LVACALTEGETRDAWDIVLSRRPARCIPYASVMTLPATGSEMNANSVISRLETKEKLA
                     FVAPENPRFSILDPECCRSLGAAQVAASLADITAHVLEQYM"
     gene            complement(755..1537)
                     /locus_tag="ACZPB6_08520"
     CDS             complement(755..1537)
                     /locus_tag="ACZPB6_08520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1184548.1"
                     /translation="MEKKLLVLGMAAMMAAGAANAELQAFKVNGETVSVAQQKALYDS
                     AVAQGQPAGAELERQVKNVLTQQTVLLQEAKKAKLDRNSDVKQAIEAARNQVLINAVL
                     RDWAKKNPVKDEDVRKAYEAEKAAYGPTEYQVRHILVKTEDQAKNIISRLNKGADFDK
                     LAKEFSEDRGNKDRGGQLGWVVPRSFVPTFGNAFAGMKEGTVSKEPVRTQFGFHVVKL
                     DAKRPAQLFPTFEAQKPALVNALTNQRVNQHFGELVKNAKVQ"
     gene            complement(1649..2257)
                     /locus_tag="ACZPB6_08525"
     CDS             complement(1649..2257)
                     /locus_tag="ACZPB6_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HIR1184549.1"
                     /translation="MKLLFDLFPVILFFAAFKIAEGQPDAAIALAERFMGEGLTSMTA
                     PVFIATVVAIAATFLQVGWLMLRGRKVEPMLWISLAVIVVFGSLTLYLRNEMFIKWKP
                     TILYWIFGGILVYGNFAGRNFIRMLLSKAEIEMPGHAWGTLQNMWIGFFAAMGVVNLL
                     VAYLCSTDTWVNFKLFGLMGLTLVFTIGIGVFMTKHAVTRQN"
     gene            2491..>3472
                     /locus_tag="ACZPB6_08530"
     CDS             2491..>3472
                     /locus_tag="ACZPB6_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR1184550.1"
                     /translation="MQFARKIVLLSAVVMMGAAGGAAAKENDLTNATKPVSVVTVEGM
                     TEYRLPNGLRVVLYPDMSKPTATVNMTYLVGSRHENYGETGMAHLLEHLMFKGSKNYP
                     NPTAEFTKRGFRMNGSTWLDRTNYHVSFTASDDNMKWALGWQADAMINSFIARKDLDT
                     EMTVVRNEYEMGENRPTSVLLKRMQSVLFDWHSYGRSTIGARSDIENVEIENLQAFYR
                     RYYQPDNAVLTVSGRFDTDEVLGWIAELFGPIAKPERVLPKEWTVEPAADGEREFTVR
                     RPGETQMLMIGYRLPAALHPDWEPAAMATSILSDAPTGRLYKELVETGLAT"
BASE COUNT          639 a          986 c         1175 g          672 t
ORIGIN      
        1 catgtactgc tcgagcacgt gcgcggtgat gtcggcgagg gacgcggcga cctgggcggc
       61 gccgagcgag cggcagcact cggggtcgag aatcgagaag cgcggatttt caggcgcgac
      121 gaaggcaagc ttctccttcg tctcaaggcg ggagatgacg ctgttggcgt tcatttcgga
      181 gcccgtggcc gggagcgtca tgacgctcgc gtagggaatg cagcgggcgg gacggcggga
      241 gaggacgatg tcccaggcgt cgcgcgtttc gccctcggtg agggcgcagg caacgagctt
      301 ggaaccatcg agcacggagc cgccgcccac ggcgaggatg aagcccgcct tctgttcgcg
      361 gccgaattcg atgcacttcc tgagggtttc gaccttgggg ttggcctcga tgccccagaa
      421 ttcgacgtgg tcgatgccgg cgagcgcggc cttgacttgg tcgtagacgc cgttgcgctt
      481 cacggagccg ccgccgaagg tgacgaggac gcgcacgccc tcggggatgt ggcgggtgag
      541 ggaggcgatt tcgccgcggc cgaaaatgag tcgggtcgga ttgtaatact cgaagttctg
      601 catgagggag ccttgaaaaa agtggttttg ctgacagtat acggagccgg gccgcgggtg
      661 gaaaggcgcg ttttcgctca atgcagaagg gggaggtccg cgatgcgggc ctcccccttg
      721 ggttcgtgtc gagtcgtcgg ggacgaagga tccgttactg gaccttggcg ttcttgacga
      781 gttcgccgaa gtgctggttg acgcgctggt tcgtgagcgc gttgacgagc gcgggcttct
      841 gagcctcgaa ggtcgggaag agctgggccg gacgcttggc gtcaagcttc acgacgtgga
      901 agccgaactg cgtgcgcacg ggttccttgc tgaccgtgcc ttccttcatg ccggcgaagg
      961 cgttgccgaa ggtcggaacg aaggagcggg gaacgaccca gccgagctgg ccgccgcgat
     1021 ccttgttgcc gcggtcttcg gagaattcct tggcgagctt gtcgaagtcg gcgcccttgt
     1081 tgaggcggct gatgatgttc ttggcctggt cttcggtctt gacgaggatg tggcggacct
     1141 ggtattcggt cgggccgtag gcggcctttt cagcctcgta ggccttgcgg acgtcttcgt
     1201 ccttgaccgg gtttttcttg gcccagtcgc gcaggacggc gttgatgagg acctggttgc
     1261 gggcggcttc gatggcctgc ttgacgtcgg agttgcggtc aagcttggcc ttcttggctt
     1321 cctgaagcag gacggtctgc tgcgtgagga cgttcttcac ctggcgctca agttcggcgc
     1381 ctgcaggctg gccctgagcg acggcgctgt cgtagagtgc cttctgctgg gcaacgctga
     1441 cggtttcacc gttgaccttg aaggcctgaa gttcagcgtt ggccgcgccg gcggccatca
     1501 tggcagccat gccgagaacc aaaagtttct tttccatttc tttcttcctt tactgccgca
     1561 ggtgttcggc gcttcccggc accgtacgcc gctatcaggt tgttagtcat tgcacccgaa
     1621 attataaggg gttcgggcgt catgcaggtc aattctgcct agtgacggcg tgtttggtca
     1681 tgaacacgcc gatgccaatt gtaaagacta gtgtcaggcc catcaggccg aagagcttga
     1741 agttgaccca ggtgtcggtc gaacagaggt aggcgacgag gaggttgacg acgcccatgg
     1801 ccgcgaagaa gccgatccac atgttctgga gcgtccccca ggcgtggccg ggcatttcga
     1861 tctcggcctt ggagagaagc atgcggatga agtttcggcc cgcgaagttg ccgtagacga
     1921 ggatgccgcc gaagatccag tagaggatcg tgggcttcca cttgatgaac atctcgttgc
     1981 gaagatagag cgtgaggctg ccgaagacga cgatgacggc gaggctgatc cagagcatcg
     2041 gctcgacctt gcggccgcgt agcatgagcc agcccacctg caggaaggtg gcggcgatgg
     2101 cgacgacggt ggcgatgaag accggagcgg tcatggaggt gaggccctcg cccataaagc
     2161 gctcggcaag cgcgatggcg gcgtcgggct ggccctcggc gatcttgaag gccgcgaaga
     2221 agaggatgac ggggaagagg tcgaagagaa gtttcatcgt aaggaggttc atgtggagcg
     2281 ggatcctgaa attttagcga aattggcagt tgatgtttca gggggaggcc ctggggccga
     2341 aggcttggta acatttcgaa cattgttcag gtgcttcgaa acgttttcgt cacctgggac
     2401 aggtatcgtt gtgcagtcgg gggctctcgg ccgtcagggg acggtttgtg acgcgctccc
     2461 gcaacacgat atgaacaacg gactttcgtt atgcaatttg caagaaaaat agtgctgctt
     2521 tccgccgtcg tgatgatggg cgccgcagga ggcgcggcag ccaaggagaa tgatttgacg
     2581 aacgcaacca aacccgtttc cgtcgtgacc gtcgagggca tgaccgagta caggctcccg
     2641 aacggcctgc gcgtcgtgct ctatcccgac atgagcaagc cgaccgccac ggtcaacatg
     2701 acctatctcg tgggctcgcg tcacgagaac tacggcgaga ccggcatggc ccaccttctc
     2761 gaacacctga tgttcaaggg gtcgaaaaac tatccaaatc cgaccgccga gttcacgaag
     2821 cgcggcttcc gcatgaacgg ctcgacgtgg ctcgaccgca cgaactacca cgtctccttc
     2881 acggcctcgg acgacaacat gaagtgggcg ctcggctggc aggccgacgc catgatcaac
     2941 tcgttcatcg cccgcaagga cctcgacacc gagatgaccg tcgtgcgcaa cgagtatgaa
     3001 atgggcgaga accgtccgac gagcgtgctc ctcaagcgca tgcagtccgt gctcttcgac
     3061 tggcacagct acggccgcag cacgatcggc gcccgaagcg acatcgagaa cgtcgagatc
     3121 gagaacctcc aggccttcta ccgccgctac taccagcccg acaacgccgt gctgacggtt
     3181 tcgggcaggt tcgacacgga cgaggtgctt ggctggatcg ccgagctctt cggcccgatc
     3241 gccaagcccg agcgcgtgct tcctaaggag tggacggtcg agcccgccgc cgacggcgag
     3301 cgcgagttca cggtccgccg tcccggcgag acgcagatgc tcatgatcgg ctaccgcctg
     3361 cccgccgccc tccatcccga ctgggagcct gcggccatgg ccacgtcgat cctttccgac
     3421 gccccgacgg ggcgcctcta caaggagctc gtcgagacgg gccttgccac gc
//
LOCUS       DBMDTI010000211         3442 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6245_length_3442_cov_2.660581, whole genome shotgun sequence.
ACCESSION   DBMDTI010000211 DBMDTI010000000
VERSION     DBMDTI010000211.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6245_length_3442_cov_2.660581"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="ACZPB6_08535"
     CDS             <1..319
                     /locus_tag="ACZPB6_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184551.1"
                     /translation="RLVWAEGLRSPEIMHDLYARLFVPVFRNDGLLACMLEKEDVPDF
                     VRNRPVVLVAPLIQSIFIHVLVGNELIGSPREYISDYFDFILSKNAREEHVPDVRLPG
                     EDA"
     gene            536..1726
                     /locus_tag="ACZPB6_08540"
     CDS             536..1726
                     /locus_tag="ACZPB6_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HIR1184552.1"
                     /translation="MTDTGEKTFFMRWLPVLTLTLCTFVFNTSEFIPIGLLTDIGRDF
                     GRTEAEMGWLVTTYAWVVALMSLPLMLLASKMECRRLMMSVLALFVASHVLSAVASNY
                     WILLASRLGVACSHAIFWSVVSPLAVEVAPKHKTSAALGLVVAGSSIAMIAGLPLGRV
                     LGLYLGWRMTFLAIGLVAAIALVCLWRFFPSVHSRNAVALDKVPALLSRPALMGIYIL
                     TPLIMTGNFTVYSYIEPFLAQVTGMLPDRITWVLVAYGAVGILGSWIFSHFFDRRPIG
                     FLQFSVFGIVASLFLMAPLGGGELTIVLLCIFWGLAVTLYNLVFQSAIIRAVPSGTAV
                     AMSVYSGIYNVGIGSGALVGGLVCTHASIADIGWAGGAIAAAGALFCLLRVGPVMRGV
                     FAKS"
     gene            complement(1698..2849)
                     /locus_tag="ACZPB6_08545"
     CDS             complement(1698..2849)
                     /locus_tag="ACZPB6_08545"
                     /inference="COORDINATES: protein motif:HMM:NF047202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOTE conflict system archaeo-eukaryotic primase
                     domain-containing protein"
                     /protein_id="HIR1184553.1"
                     /translation="MEASSLISSSEKAKPLPKGKRKAEFVHQEGSPPLQTAQGLSRAF
                     GYTRKILKLPEMRHGRREGSTCPFVTRVVTDLQKVVLELKAEISALRSQSSLPKAVEP
                     LKAVETPPATATSGLEIPDEPWTDAEAKTQAPCGSTGKQLVKTPKIVAETDLTSREKV
                     ELFAGLFAGRSDVYATRWASRTDAQKSGYSPKCTMRYTEGCSLKCHLCPHRAYEKVTA
                     GTYYFHLKGDIVMGVYPLLTDNTCRFAVLDFDNSDWERDGKAVIKTARKHGIPLVPEI
                     SRSACGVHLWLFLSVPMQAAVVRRVFERLIALTIADEGLLKLDSFDRIIPCQDELPRG
                     NSSIGNLVALPMQPEAKARGGSSFIRRDARLSFMRQARMHLRTSRRHRA"
     gene            2956..3402
                     /locus_tag="ACZPB6_08550"
     CDS             2956..3402
                     /locus_tag="ACZPB6_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR1184554.1"
                     /translation="MLLPSVFGEDLFDETFPFAGMTDRHGHDKLFGNRAGRLMKTDVR
                     EGEKSWELDVELPGFRKENISATLKDGYLTISAEKAVEETQEAEKKGQCVRRERWSGA
                     LSRAFYVGEGVTEEDVKAKYENGVLSISIPKKEAIEAKAKKMIAIE"
BASE COUNT          610 a         1058 c         1023 g          751 t
ORIGIN      
        1 ccgactcgtc tgggccgagg gcctgcgctc gcccgagatc atgcacgacc tttacgcgag
       61 gctcttcgtg cctgtcttca gaaatgacgg ccttcttgcc tgcatgctcg agaaggagga
      121 cgtccccgac ttcgtgcgca accgtcccgt cgtcctcgtc gcgccgctca tccagtcgat
      181 cttcatccac gtgctcgtgg gcaacgagct catcggctcg ccccgcgagt acatcagcga
      241 ctacttcgac ttcatcctct cgaagaacgc acgcgaggag cacgtccccg acgttcgcct
      301 gcccggcgaa gacgcctgac gagcggccct caagcttcat tcggggtggg gcgcaaggcc
      361 gcccgcctca cacaaagcaa gtctcccgca gcggcttgat gtgcgtcatt gacgccgctt
      421 caggccgctg ccttcgccgg acggctgcgc taagattctc atccgccaaa aaaaacgcag
      481 gtcccgcctg cggatgaaaa cgacagtccc ggacaaaatc aaagcgcaca tcatcatgac
      541 cgacaccggc gaaaagacct tcttcatgcg ttggcttccc gtattgacgc tcacgctctg
      601 caccttcgtc ttcaacacct ccgaattcat tccgatcggg cttctcaccg acatcggccg
      661 tgacttcggc cgcaccgagg ccgagatggg ttggctcgtg acgacctacg cctgggtcgt
      721 cgcgctcatg tcgctgccgc tgatgctgct cgcctcgaag atggagtgtc gtcgcctcat
      781 gatgtcggtg cttgcgctct tcgtcgcgag ccacgtgctc tcggccgtcg catccaacta
      841 ctggattctc ctcgcgtcgc gccttggcgt cgcctgctcg cacgcaattt tctggtccgt
      901 cgtgagcccg cttgccgtcg aggttgcacc gaagcacaag acctccgccg cgctcggcct
      961 tgtggtcgcg ggttcgtcca tcgccatgat cgcgggcctg ccgctcggac gcgtgctcgg
     1021 cctctatctg ggctggcgca tgaccttcct cgccatcggg cttgtcgcgg cgattgcgct
     1081 cgtctgcctc tggcgctttt tcccgagcgt gcacagccgc aatgccgtgg cgctcgacaa
     1141 ggtgccagca cttctaagcc ggcccgctct catgggcatc tatatcctga cgccgctcat
     1201 catgacgggg aacttcacgg tctactcgta catcgagccc ttcctcgcgc aggtgaccgg
     1261 catgcttccc gaccgcatta cctgggtgct cgtcgcctac ggcgccgtgg gcattctcgg
     1321 cagctggatc ttctcgcact tctttgaccg ccgtccgatc ggcttcctgc agttctcagt
     1381 gttcggcatc gtcgcgagcc tctttctcat ggcgcctctg ggcggcggcg aactcacgat
     1441 cgtgctgctc tgcatcttct gggggcttgc ggtgacgctc tacaacctgg tcttccagtc
     1501 ggccatcatc cgtgccgttc cgtcgggcac ggccgttgcc atgagcgtct attcgggcat
     1561 ctataacgtg ggcatcggtt cgggcgccct cgtgggcggc ctcgtctgca cccacgcctc
     1621 gatcgccgac atcggttggg cgggcggtgc cattgcggcc gcaggcgcgc tcttctgtct
     1681 cctgcgcgtc ggtcccgtca tgcgcggtgt cttcgcgaag tcctgagatg catgcgggct
     1741 tgacgcatga atgaaaggcg ggcgtccctt cgaatgaagg atgaaccacc gcgcgccttg
     1801 gcttcaggct gcatgggaag cgccaccaga ttgccgatgc tgctgttgcc gcgcggcagt
     1861 tcgtcctgac agggaatgat gcggtcgaag gagtcgagct tgagtagccc ctcatcggcg
     1921 atcgtgagcg caatcagtct ttcaaaaacc cgtctgacca ctgcggcctg catgggtacg
     1981 gacaggaaga gccacaggtg cacgccgcag gcggatcggg agatttcagg aacgagtgga
     2041 atgccgtgct ttctggcggt cttgatgacg gctttgccgt cacgctccca gtcactgtta
     2101 tcaaagtcaa gaacggcaaa tcggcaggtg ttgtccgtca ggagcggata gacgcccatc
     2161 acgatgtcgc ctttgagatg gaagtagtag gtcccggcag tcaccttttc gtaggctcgg
     2221 tgcgggcaca ggtggcactt caggctgcag ccttcggtgt aacgcatggt gcatttcggc
     2281 gagtagccgg acttttgcgc gtcggtcctg gaggcccagc gggttgcgta gacatcggat
     2341 ctgcctgcaa atagacctgc gaacagctcg accttctctc gtgaagtgag atccgtttcc
     2401 gcaacaatct tcggcgtctt gaccaattgt tttccggtgc tgccgcatgg ggcttgtgtt
     2461 ttggcttcag cgtccgtcca cggctcatca ggaatctcga ggcctgacgt ggcggttgcg
     2521 ggcggggttt cgactgcttt gagcggttcg acggctttgg gcaaactgct ttggctgcgc
     2581 agcgccgaga tttccgcctt gagttcgagc acgactttct gaaggtcagt gacgacccgt
     2641 gtcacaaaag ggcaagttga gccttcacgt cttccatgac gcatctccgg gagcttcagg
     2701 atttttcgag tatatccgaa ggctctcgac aatccttgag ccgtctgaag aggaggggag
     2761 ccttcctgat gcacgaactc cgcttttctt ttgcctttag gcaaaggttt cgctttttcc
     2821 gagcttgaaa tcagacttga agcctccata tagtcctcag agagaggtgc ggggcgcccg
     2881 aaagggtgcc gcacaggcac ttctccgaat gcacaccgcc atggtcgacg gcagaagaca
     2941 atgaaggaaa acaaaatgct gcttccgtct gtttttggtg aagacctttt cgatgaaacg
     3001 ttcccgttcg ccggcatgac cgatcgtcac ggtcatgaca agctcttcgg caaccgcgcc
     3061 ggccgtctga tgaagaccga cgtgcgcgaa ggcgaaaaga gctgggagct tgatgttgag
     3121 cttccgggct tccgcaagga gaacatcagc gccacgctca aggacggcta tctgacgatc
     3181 agcgctgaaa aggccgttga ggagacgcag gaggccgaaa agaagggcca gtgcgttcgc
     3241 cgcgaacgct ggtccggcgc tctgagccgc gcgttctacg tgggcgaggg cgtgactgaa
     3301 gaagacgtca aggcgaagta cgaaaacggc gtcctgagca tctcgatccc gaagaaggag
     3361 gcgatcgaag ccaaggccaa gaagatgatc gccatcgaat gaggtcattt ctctggtttt
     3421 cgtcgatgca ttgacgcatg aa
//
LOCUS       DBMDTI010000212         3431 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6264_length_3431_cov_1.846847, whole genome shotgun sequence.
ACCESSION   DBMDTI010000212 DBMDTI010000000
VERSION     DBMDTI010000212.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6264_length_3431_cov_1.846847"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(264..662)
                     /locus_tag="ACZPB6_08555"
     CDS             complement(264..662)
                     /locus_tag="ACZPB6_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional initiation protein Tat"
                     /protein_id="HIR1184555.1"
                     /translation="MDRRTLLGALGLALAAPLGSALAAEPPKYTRPGRVYNQNGAYEG
                     RMDSSGRMYDQSGRYVGREDSSGRRYDASGRYQGRTDADGRRYDSSGRYQGHQDSSGR
                     WYDQSGRYQGRESRDGSRYDSSGRYQGRTR"
     assembly_gap    766..775
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(900..1907)
                     /locus_tag="ACZPB6_08560"
     CDS             complement(900..1907)
                     /locus_tag="ACZPB6_08560"
                     /inference="COORDINATES: protein motif:HMM:NF024676.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1184556.1"
                     /translation="MPRKSSTLDEAVMLIQILTRIPKGRLITAQQLKQELDAAGIPIR
                     IRTLQRYLKTMASTDVFGIDCDMRSRPYGYKQSTAGGTLLSQQMSVHECLLLRLAQEH
                     MRFMIPTTLSKSLSPLFDMASQKFNETLSAAPRNEKAWLKKIAVVGSSLPMMPPKISR
                     TIFEAVSEALFRELKLEIEYDSASGKQVKSLITPLGLVQQDVRLYLVCMFDRYENVRH
                     LALHRLTKARVTEFQAERPKDFSLDSYVNSCHFNYSDGSGRKVELSFRFTNAVTALNL
                     NETPFNRTQRLEKLPDGSFHLSVILTDSPLIDGWIAVWKDKAGISELTRTPVMGTPQE
                     N"
     gene            complement(1979..2788)
                     /locus_tag="ACZPB6_08565"
     CDS             complement(1979..2788)
                     /locus_tag="ACZPB6_08565"
                     /inference="COORDINATES: protein motif:HMM:NF021631.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase beta superfamily protein"
                     /protein_id="HIR1184557.1"
                     /translation="MTSTAEEMFDEAKARLRRLEASHGIRILYACEAGSRSWGFPSPS
                     SDYDIRFIYVRPLKNYLKLTPPADTITLDQDGVWDISGWDLKKCLMLLKKGNVSIVQS
                     LYSQIVYRNHPLFLAEMRKLLDYGAPLPRLYWAYRSMAQSHVSHFLLGHETIAYKRFL
                     YIVQGLLAMRWVETRKSMPPVVFEQLADALVTDEGLRAEIETLLNIQRSAGALEETGP
                     KTLFPGVLSFIETTLAQTQPPADQAIDINEEVLDDFYLKWLGVPGLSHPGA"
     gene            complement(3051..>3431)
                     /locus_tag="ACZPB6_08570"
     CDS             complement(3051..>3431)
                     /locus_tag="ACZPB6_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184558.1"
                     /translation="DAPFPPTARGASEPDARLRAARFMLETIRPIPEDGEAREKRISL
                     LTEPLHSGALPDAAFVRRVLYLAGRWYHRPDQMDAEGALMLRKYLLRVWPEYAGEPVT
                     TKLIRLWRTSEYVIGTVTVNSTLN"
BASE COUNT          620 a          915 c         1199 g          687 t
ORIGIN      
        1 tagatcggaa gagagaggtt tgccgcattc ctccatccga atgaattcgg aggcttcctg
       61 cggctgtttc tgtgaaaagg tcctcctgct gaaggaggaa catgtgcatc tattcaagaa
      121 tagacgtcgg ggagcaggcg ggcgcgaacc gaatgcgcaa agaaaaccac tctaattgac
      181 gggcgtcaag acaacaaaac cgctattctg ctactgaata gcggtttttc gaggtgcgtg
      241 agttttgggc aggtgagcgg ccgtcagcgg gtcctgccct gatagcgtcc ggagctgtcg
      301 tagcggctgc cgtcgcggga ctcgcgtccc tggtagcggc cggactggtc gtaccagcgt
      361 ccgcttgagt cctggtgtcc ctgatagcgt ccggagctgt cgtagcggcg cccgtcggcg
      421 tcggttcggc cctggtagcg gccggaggcg tcgtagcgtc gtccgctcga atcctcgcgc
      481 ccgacgtagc ggcccgactg gtcgtacatg cgtccgctcg agtccatgcg gccctcgtag
      541 gcaccgttct ggttgtagac gcggccgggc ctcgtgtact tcggaggctc ggcggcgagt
      601 gcgctgccaa gaggcgccgc aagggcaagg ccgagggcgc cgagaagcgt tcttctgtcc
      661 atgtcaataa tcctgtggtg cgatgccgct gcctgtgacg ggcgttcggg tcagctcgga
      721 gatgcccgcc ttgtccttcc agacggcgat ccagccgtcg atgagnnnnn nnnnnggatg
      781 aacatcccac ggtgcctgta cattcccaac caacgtagct cgcctccccg aggaggtttg
      841 ctgagactag tcaataaagg ctttttcaag cctccgcctt taggcggggg ttattgacgt
      901 cagttttcct gtggtgtgcc catgacgggc gttcgggtca gctcggagat gcccgccttg
      961 tccttccaga cggcgatcca gccgtcgatg aggggggagt cggtcagaat gacgctcagg
     1021 tggaagcttc cgtcggggag cttctcgagc ctttgcgtcc tgttgaaggg cgtctcgttg
     1081 aggttgaggg ccgtcacggc gttcgtgaag cggaaggaga gctcgacctt gcggcccgag
     1141 ccgtccgagt agttgaagtg gcacgagttg acgtaggagt cgagcgaaaa gtccttcgga
     1201 cgctcggcct gaaattcggt gacgcgggcc ttcgtgaggc ggtgaagcgc gaggtggcgc
     1261 acgttctcat agcggtcgaa catgcagacg aggtagaggc gaacgtcctg ctgcacgagg
     1321 ccgagcgggg tgatgaggct tttgacctgc ttgcctgaag cactgtcgta ctcgatctcg
     1381 agcttgagtt cacgaaagag cgcctcgctc acggcctcga agatcgtgcg ggagatcttc
     1441 ggcggcatca tcggcaggga gcttcccacg acggcgatct tcttgagcca ggccttctca
     1501 ttgcgcgggg cggcggagag agtctcgttg aacttctgcg aggccatgtc gaacaggggc
     1561 gagagggact tcgagagcgt cgtcgggatc atgaagcgca tgtgctcctg ggcgagcctc
     1621 aggagcaggc attcgtgcac ggacatctgc tgcgagagga gcgtgccgcc cgccgtgctc
     1681 tgcttgtagc cgtaggggcg ggagcgcatg tcgcagtcga tgccgaagac gtccgtcgag
     1741 gccatggtct tgagatagcg ctgcagggtg cggatccgga tcggaatgcc cgcggcgtcg
     1801 agctcctgct tgagctgctg tgccgtgatg aggcggccct ttggaatgcg ggtgaggatc
     1861 tggatgagca tcacggcttc atccaaagtg gaggatttgc gcggcatgta ctgctccgtt
     1921 gaaagagaag aagggcgccc ttggtcaagg cgccctcctg ttttgtgtgg tgacgggatc
     1981 aggcgccggg gtgcgaaagt cccggaacgc cgagccactt gagatagaag tcgtcgagca
     2041 cttcctcgtt gatgtcgatc gcctggtcgg cgggcggctg cgtctgtgcg agcgtcgtct
     2101 cgatgaagct cagcacaccg gggaagagcg tcttgggacc cgtttcctca agggcgcctg
     2161 cgcttcgctg aatgttgaga agcgtctcga tctccgcgcg aaggccctcg tccgtgacga
     2221 gcgcgtcggc gagttgctcg aagaccacgg gcggcatgct cttgcgggtc tcgacccagc
     2281 gcatggcgag gagcccctgc acgatgtaga ggaagcgctt gtaggcgatc gtctcgtggc
     2341 cgagcaggaa gtgcgagaca tgggactgcg ccatcgagcg gtaggcccag tagaggcgcg
     2401 gcagcggggc gccgtagtcc agaagcttcc tcatctcggc gaggaagagt ggatgattgc
     2461 ggtagacgat ctgcgagtag aggctctgca cgatcgagac gttgcccttc ttgaggagca
     2521 tcaggcactt cttgagatcc cagcccgaaa tgtcccacac gccgtcctga tcgagtgtga
     2581 tcgtgtcggc cgggggcgtg agcttcagat agttcttgag cggacgcacg tagatgaagc
     2641 ggatgtcgta gtccgagctc ggggacggaa agccccagct tcggctgccc gcctcgcagg
     2701 catagaggat cctgatgccg tgggaggctt cgaggcgcct gagccgcgcc ttcgcttcgt
     2761 caaacatttc ttcagcagtg gaagtcatag tgagggcctg aaaatccgta cgtttccgac
     2821 agaagaggta acgtacgcgt caattgagtc ttggatcgtg cacgttatat cgtgctatat
     2881 atcttcggca tcgaacggtg gaaaatcatg ccgaaattct tgatcggccc attatcgcac
     2941 gaagagccgc gaggagtgtc ctatgaagac ggaattgttg gcggcgagga cggccatgta
     3001 tccaatatgc aacggggcga gcgctctgtc ggaaggattt cggcagaacg tcagttgagc
     3061 gtggagttga ccgtgacggt accgatcacg tattcggagg tgcgccagag gcggatgagc
     3121 ttggtggtga cgggttcgcc cgcgtactcg ggccagacgc gaaggaggta cttccgaagc
     3181 attagggcgc cctcggcatc catttggtcg gggcggtggt accagcggcc ggcgagatag
     3241 aggacgcgtc gcacgaaggc ggcgtcggga agcgcacccg aatgaagggg ctcggtgaga
     3301 agggagatgc gcttttcgcg tgcttcgccg tcctcgggga tcgggcggat cgtctcgagc
     3361 atgaagcgtg cggcgcgaag gcgggcatcg ggttcggatg cgccccgtgc ggtaggcggg
     3421 aagggcgcgt c
//
LOCUS       DBMDTI010000213         3428 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6270_length_3428_cov_3.170424, whole genome shotgun sequence.
ACCESSION   DBMDTI010000213 DBMDTI010000000
VERSION     DBMDTI010000213.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6270_length_3428_cov_3.170424"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            486..629
                     /locus_tag="ACZPB6_08575"
     CDS             486..629
                     /locus_tag="ACZPB6_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184559.1"
                     /translation="MIEWRGPLARTPERASSAAAFLSLTLCAFFGILKTKGALMGFGN
                     GTI"
     gene            633..1766
                     /locus_tag="ACZPB6_08580"
                     /pseudo
     CDS             633..1766
                     /locus_tag="ACZPB6_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013132.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
     gene            complement(1854..2396)
                     /locus_tag="ACZPB6_08585"
     CDS             complement(1854..2396)
                     /locus_tag="ACZPB6_08585"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR1184560.1"
                     /translation="MAVDLFNRHFDFPVLLAIREAFESGTWDGWMRAVTALGDHGAVW
                     IALGVALLLFRRTRLTGAAILLALTLVALVGNMGLKPLFDRLRPCDIMGFTLLPACPP
                     DPSFPSGHTAASFASATVLWLRRSAFFRPALVLAALIAFSRLYLFVHFPTDVMAGILL
                     GLCCAWCAARGLEFIADRRR"
     gene            complement(2405..2872)
                     /gene="nrdG"
                     /locus_tag="ACZPB6_08590"
     CDS             complement(2405..2872)
                     /gene="nrdG"
                     /locus_tag="ACZPB6_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1184561.1"
                     /translation="MNYIGLSTCDTANGPGVRVSLFCSGCTLHCPGCFNEESWDFGAG
                     RPFTDETLATLLKALEEDFVEDLSLLGGDPLEPENRDEVLRIVRAVRERFGSTKTIWL
                     WTGRRHEKVADCPVLEYVDVLVDGPYVEKLKVHEKGYWFGSSNQRVIPLHPAG"
     gene            complement(2883..>3428)
                     /gene="nrdD"
                     /locus_tag="ACZPB6_08595"
     CDS             complement(2883..>3428)
                     /gene="nrdD"
                     /locus_tag="ACZPB6_08595"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR1184562.1"
                     /translation="LAQFKKEDGHLYAIYGTPAESLCATQARQYDRFCRDEGVENVFR
                     STEHYSPEYFTNSFHVNVTEDITPFEKQDREFTDFHLCEGGHIQYVRLDNPENFEAVK
                     AVIRRGMKKGFYQGVNFDSAYCGDCGRHSTNVLFKCPHCGSANLSVISRVCGYLGYSN
                     VNGMSRMNDGKMAEIRNRKSM"
BASE COUNT          610 a         1114 c         1065 g          639 t
ORIGIN      
        1 aaagagcgga ttgaaaatga agcaagggtg tcgcgagcac accacggccg ccaactacgc
       61 cgcctggggc cgctactgcg agctctacgg ccccacgccc gacacgctca tcaccatgtg
      121 cggcgcaggc atcaagtccg accactgacc cgcacgctca gccgttgcgg ctccctgcac
      181 ctcagggaac cgcaacgcgc atccgccccc ttcaccatcc tccctgcggc ctcctcccgc
      241 cttcccatcc tgcttcctga tgcctcggac accgccttca agtgctgacg gagagcacgt
      301 tccccgagta ttttgactca agacaaaacc ctgtcccacg aagggaatac gccgtaggca
      361 aaacgcctta gggctgtcat ttataagaga cgtgaaattt cagttgaatt gcaaaccttc
      421 tttgacggct tgacgcaggc cacaagacgg cgttttgacg tccgaatccg tcctcgagca
      481 ggcacatgat tgaatggcgc gggcccctcg cccgcacacc cgaacgcgct tcgagcgccg
      541 ccgccttcct gtcgctcacg ctatgcgcct tcttcggcat tctgaagacg aagggcgcgc
      601 tgatgggctt cggcaacggc acgatctagc tcatcgtctg cgcgttcttc ctctcgcgcg
      661 gcttcatcaa gatgaggctc ggccgccgca ttgcgtttcg catcattcag gcgatcggca
      721 ggagcgccac gtcgctcggc tattcgatct gtctcgcaga actcatcatc tctcccgcca
      781 tgccgtcggc cacgacccga ggaggaggcg gcgtcttcta tccgatcgtc cagtcgctct
      841 cgagcgcctt cgactccgaa cccgacccca ccgcaggccg catcggcaag tacctgatgc
      901 aggtcggctt ccatgccgac gccgtgacct gcataatgtc cctcacctcg atggccggca
      961 acccgctctg cgtgggtctg gccgcgagcg ccatcggcgt cgagctcacc tgaatgcagt
     1021 gggcggtcgc cgccatcgta ccgggcctcg tctgcctgct cctcgtgccg ctcgtcctcc
     1081 tcaggattgc gccgcccgaa atcaggcaga ttccgaatgc acgtcagatc gcgcgtgacg
     1141 aactcgagaa gatggggccg aactcgtgct ctccttcgtc ttcctgatgt gcctcgtgct
     1201 ctggcctcac gggtctgggt gccacgtcga tcgcgatgct cgcggtctcc atcatgctca
     1261 tcgccggcgt cctcacctgg aaggacatcc tcgccgaaaa gggcgcctgg gacgcgatgt
     1321 tctggatggg cgggctgatg gcgctcgcca cggtcctctc caaatccggc ttcatcaagt
     1381 gggccgctgc cttcattgcg gacggcatcg cggacgcgca catgggctgg atctcctcct
     1441 tcctgatcat ctcgctcatc tacatctact cgcactactg cttcgcttcg gtgacggcca
     1501 gcatctccgc catgtatgcc gccttcgtcg ccgtcgcggc ggcctgcggc gcccccgccc
     1561 tgatggtcgc ggtcgccttc ggcatcttcg ccaaccttcc catctcgctc acccactacg
     1621 gcaacggcgc cgtccccgtc tatttcggca caggctacgt gagccagagc gaatggtggc
     1681 gcaacggctt catcgtctgc acggtcaaca ccgtcgtgtg gctcaccgtc ggcatgacct
     1741 ggtggaaact catcggcctc tggtgaccgg agcttgatca aacggcccga tcggtccgca
     1801 tccctcgggg atgcggacct ttttcgtcat ggaccgtacg gttcgatgag gcatcagcgg
     1861 cgcctgtcgg caatgaattc aaggcccctc gcggcgcacc aggcgcagca gagcccgagc
     1921 aggatgcctg ccatgacgtc cgtagggaag tgcacgaaga gatagaggcg ggagaaggcg
     1981 atgagggccg ccagcacgag ggcgggccgg aagaaggccg agcggcgcag ccacagcacg
     2041 gtggccgacg caaaggaggc ggccgtgtgg cccgagggga aggaaggatc gggcggacac
     2101 gcgggcagaa gcgtgaagcc catgatgtcg cagggacgaa gtcgatcaaa gagcggcttg
     2161 agtcccatgt tgccgacaag tgcgaccaga gtgagcgcga gaaggattgc ggctcccgtg
     2221 agcctcgttc tgcgaaagag gagaagagcg acgccgagcg cgatccagac ggcgccgtga
     2281 tcgcccagcg cggtgaccgc acgcatccag ccgtcccagg tgcccgactc gaaggcctcc
     2341 ctgatggcga gaagcacggg aaagtcgaag tgacggttga agagatcgac ggccatggaa
     2401 cgaatcagcc agcgggatga aggggaatga cgcgctggtt ggagcttccg aaccagtagc
     2461 ccttttcatg caccttgagc ttttcgacgt agggaccgtc gacgagcacg tcgacgtact
     2521 cgagcacggg gcagtccgcc accttctcgt ggcggcggcc cgtccagagc cagatggtct
     2581 tcgtgctgcc gaacctttcg cgcacggccc tgacgatcct caggacctcg tcccgattct
     2641 cgggttctag cggatcgccg ccgagcaggc tgaggtcctc gacgaagtcc tcctcgagcg
     2701 ccttgagaag cgtcgcgagg gtttcgtcgg tgaagggcct cccggctccg aagtcccagc
     2761 tctcctcgtt gaagcagccg ggacagtgca gcgtgcaccc cgagcagaag agcgagacgc
     2821 gcacgccggg gccgttggcc gtgtcgcagg tgctcaagcc gatgtagttc atcggaggat
     2881 ccttacatgc tcttgcggtt gcggatttcg gccatcttgc cgtcgttcat gcgggacatg
     2941 ccgttgacgt tggagtagcc gaggtagccg cagacgcggg agatgacgga gaggttcgcg
     3001 ctgccgcagt gcgggcactt gaagagcacg ttcgtgctgt gcctgccgca gtcgccgcag
     3061 taggcgctgt cgaagttgac gccctgatag aagcccttct tcatgccgcg gcggatgacc
     3121 gccttgacgg cctcgaagtt ctcggggttg tcgaggcgca cgtactggat gtggccgcct
     3181 tcgcagagat ggaagtccgt gaactcgcgg tcctgcttct caaagggcgt gatgtcctcc
     3241 gtcacgttga cgtggaagga gttcgtgaag tactcggggc tgtagtgctc ggtcgaacgg
     3301 aacacgttct cgacgccttc gtcgcggcag aagcggtcgt actgacgggc ctgcgtggcg
     3361 cagagcgact cggccggcgt gccgtagatg gcgtagaggt gaccgtcctc cttcttgaac
     3421 tgcgcgag
//
LOCUS       DBMDTI010000214         3428 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6271_length_3428_cov_2.660355, whole genome shotgun sequence.
ACCESSION   DBMDTI010000214 DBMDTI010000000
VERSION     DBMDTI010000214.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6271_length_3428_cov_2.660355"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="ACZPB6_08600"
     CDS             <1..731
                     /locus_tag="ACZPB6_08600"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HIR1184563.1"
                     /translation="VMHRKIEGKVKSITLSMDACGDYWASVLYEDGLTEAVPATCVRQ
                     TKTVGVDVGIETFGTCSDGEKIKSPKALKRAEKKLKKAQRAFSRKQKGSKNREKARKR
                     LARLHRKVKRRRSDFLHKTSRRLVNDNQALIFETLKIQGMMKNHHLAKAIADAGWAEF
                     MRQCEYKAKHEGRIFAKIGTFFPSSKTCSACGHKLKELSLATRRWTCPHCGAQHDRDL
                     NASLNIKQEGIRMLKAAGLTVLRS"
     gene            867..1295
                     /locus_tag="ACZPB6_08605"
     CDS             867..1295
                     /locus_tag="ACZPB6_08605"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1184564.1"
                     /translation="MQTAPNIVHGDWKTYQHEAERVRAMVFIAEQKVPRELEFDEDDK
                     TAEHFISFDARHMVTGCARLLDNGRIGRVAVLRPFRGRGIGEAVMKTVLERAKARGMK
                     DVHLAAQVQAMPFYERLGFEPFGDEFDEAGIPHRMMRLVF"
     gene            complement(1458..2477)
                     /locus_tag="ACZPB6_08610"
     CDS             complement(1458..2477)
                     /locus_tag="ACZPB6_08610"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR1184565.1"
                     /translation="MASYKVGILGATGAVGEQMRIVLAERKFPISELRLFGSARSAGK
                     VINYEGQDIVVQEASLEAFKGLDLLLGAADNPVAKQFLPEAAKMGIHVVDNSSAFRLD
                     PDVPLVIPEVNPEDVKLSKGLIANPNCATIIGLTAVNSLHRHARIRRMVVSTYQAVSG
                     AGKAGIDALEAEVKAANGQRAEGSCFPYPIAYNLIPQIGGFNEMGYSSEEMKMQNEGR
                     KMLHDETIRFACTCVRVPIIRSHSEAITLEFENDITPDKARELLAEAPGVVLCDDPNN
                     GQYPMPLLTSDQDLVYVGRVRRDLSADAASFNRSLTLFCCADQVRKGAATNAVQIAEL
                     IFGLK"
     gene            complement(2493..>3428)
                     /locus_tag="ACZPB6_08615"
     CDS             complement(2493..>3428)
                     /locus_tag="ACZPB6_08615"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR1184566.1"
                     /translation="NFDGTFNFNETTERLKEILKKHDRFVMPGFYGRLPDGHVRVMSR
                     GGSDISGSILARCIQADLYENWTDVSGFLMADPRIVENPRNIEQITFSELRELSYMGA
                     SVLHEDAVFPLRERNIPIQVLNTNRPQDLGTIVRDKIPADPTAPLVTGIAGKKDFVSI
                     EISKKNMSNRVGFVREVLSILESYNVSVEHLPSGIDSFSVVVAKADVKQNVYEIIARI
                     QEAMKPDSIRLTEPFALIATVGRNMNSRPGTSGRLFAALGKAGINVRMIAQGSAELNI
                     IVGVENKDFNRAVAALYETFAAEAKEVRAQQAQNA"
BASE COUNT          664 a          922 c         1185 g          657 t
ORIGIN      
        1 ccgtcatgca tcggaagatc gaaggcaagg tcaagagcat cacgctctcg atggatgcct
       61 gcggcgacta ctgggcttcc gttctctacg aggacggcct caccgaagcc gtacctgcga
      121 catgcgttcg ccagaccaag acggtaggcg tcgacgtcgg cattgagacc ttcggcacat
      181 gctccgacgg tgaaaagatc aagtctccca aagctctcaa gagagccgaa aagaagctta
      241 agaaggcgca acgcgccttc tcaagaaagc agaaaggtag caagaatcgt gaaaaggccc
      301 gcaagcgtct ggcgcgactg caccgcaagg tgaagcgccg ccgcagcgac ttccttcaca
      361 agacatctcg acgattggtg aacgataacc aagcgctgat tttcgagact ttgaaaattc
      421 aaggcatgat gaagaatcac catctcgcaa aggcgattgc cgatgcagga tgggctgaat
      481 tcatgcgcca gtgcgaatac aaggcgaagc atgaaggcag aatctttgca aagatcggca
      541 ccttctttcc gtcttcgaag acatgttccg cctgcggtca caagctcaag gaattgagcc
      601 ttgcaacccg caggtggacg tgtccgcatt gcggcgcaca acatgaccgc gacttaaacg
      661 cctccctgaa catcaagcag gaaggcataa gaatgttgaa ggcggctggg ctgaccgtct
      721 tgcgctcgta acgtcacgag ccgaagcctg tggagacatg taagacccga acgatcgtcc
      781 aggcggtgtt gaagaagcag gaagcctcgc ccgatttgtt ggcaaatcgg acggggagca
      841 gtcacgatta ttcgaggaga caactcatgc agaccgcacc caatatcgtc cacggcgact
      901 ggaagaccta tcagcacgag gccgagcgcg tgcgcgccat ggtcttcatc gcggagcaga
      961 aggtgccccg cgagctggag ttcgacgagg acgacaagac ggccgagcac ttcatttcgt
     1021 tcgacgcaag gcacatggtc acgggctgcg caaggctgct cgacaacggt cgcatcggcc
     1081 gcgtcgcggt actccgtccc ttccgcggcc gcggcatcgg cgaggccgtg atgaagactg
     1141 tcctcgagcg cgcgaaggcg cgcggcatga aggacgtgca cctcgccgca caggtgcagg
     1201 ccatgccgtt ctatgagcgc ctcggcttcg agcctttcgg cgacgagttc gacgaggcag
     1261 gaatcccgca ccgcatgatg cgcctcgttt tctgaggggt gaggaaagct tcttgcgtgt
     1321 catgcaactt ttgcttttag gatcaagccg cggcgtcctt cctgtccgga ggggcgccgt
     1381 ttcgcatgcg tgaaaatgaa gcggcccgcc ttcgcagtgg aagacgggcc gtggactcag
     1441 caggtctcag gtgcgggtca cttcaggccg aagatgagct cggcgatctg aacggcgttc
     1501 gtcgcggcgc ccttgcggac ctggtcggcg cagcagaaga gcgtgagcga gcggttgaag
     1561 gacgcggcgt cggcggaaag gtcgcggcgc acgcggccga cgtagacgag gtcttggtcg
     1621 ctcgtaagga gcggcatcgg gtactggccg ttgttcggat cgtcgcagag gacgacgccg
     1681 ggagcttcgg cgaggagctc gcgggccttg tcgggcgtga tgtcgttctc gaactcaagg
     1741 gtgatggcct cggagtggga gcggatgatg ggcacgcgca cgcaggtgca ggcgaagcgg
     1801 atggtctcgt cgtggagcat cttgcggcct tcgttctgca tcttcatctc ttccgaggag
     1861 tagcccattt cgttgaagcc gccgatctgc gggatcaggt tgtaggcgat cggatagggg
     1921 aagcaggagc cttcggcgcg ctggccgttg gcggccttga cttcggcctc aagggcgtcg
     1981 atgccggcct tgcctgcgcc ggagacggcc tgataggtcg agacgaccat gcggcggatg
     2041 cgggcgtggc ggtgaagcga gttgacggcg gtgaggccga tgatggtcgc gcagttcggg
     2101 tttgcgatga ggcccttgga gagcttcacg tcctcggggt tcacttcggg gatgacgagc
     2161 ggaacatcgg gatcaaggcg gaaggcgctg gagttgtcca cgacgtggat gcccatcttc
     2221 gcggcttcgg gcaggaactg tttggcgacg ggattgtcgg cggcgccgag caggaggtca
     2281 aggcccttga aggcctcgag gctggcttcc tgaacgacga tgtcctggcc ctcgtagttg
     2341 atcaccttgc ctgcggagcg ggcggagccg aagaggcgga gttcggagat ggggaacttg
     2401 cgttcggcga ggacgattcg catctgttcg ccgacggcgc ccgtggcgcc gagaatgccg
     2461 actttgtagc tggccatgat gattgtctcc tgtcaagcgt tctgagcctg ctgggccctg
     2521 acttctttgg cttcagcggc aaaggtttcg taaagggcgg cgacggcgcg gttgaagtcc
     2581 ttgttttcga cgccgacgat gatgttgagc tcggcgcttc cctgggcgat catgcggacg
     2641 ttgatgccgg ccttgccgag ggcggcgaag agacggcccg aggtgccggg gcgggagttc
     2701 atgttgcggc ccaccgtcgc gatcagcgcg aagggttcgg tgaggcggat ggagtcgggc
     2761 ttcattgcct cctggatgcg ggcgatgatc tcgtagacgt tctgcttcac gtcggccttg
     2821 gcgaccacga cggagaagct gtcgatgccg gaaggcagat gctcgaccga gacgttgtag
     2881 gactcgagga tggagagcac ctcgcgcacg aagccaacgc ggttggacat gttcttcttc
     2941 gagatttcga tggagacgaa gtccttcttg cctgcaatgc cggtgacgag cggagccgtc
     3001 gggtcggcgg ggatcttgtc gcgcacgatc gtgcccagat cctgcgggcg gttggtgttg
     3061 aggacctgaa tcggaatgtt cctctcgcgc agtgggaaga cggcgtcttc gtgaagcacg
     3121 ctcgcgccca tgtaggagag ctcgcgcagc tcggagaagg tgatctgttc gatgttgcgg
     3181 ggattttcga cgatgcgcgg gtcggccatc aggaagccgg agacgtcggt ccaattctca
     3241 tagaggtcgg cctggatgca gcgggcgagg atggagcccg agatgtcgga gccgccgcgg
     3301 ctcatgacgc ggacgtggcc gtcgggaagc cggccgtaga agccgggcat cacgaagcgg
     3361 tcgtgcttct tgaggatttc cttgaggcgt tcggtggttt cgttgaagtt gaaggtgcca
     3421 tcgaagtt
//
LOCUS       DBMDTI010000215         3427 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6274_length_3427_cov_2.069453, whole genome shotgun sequence.
ACCESSION   DBMDTI010000215 DBMDTI010000000
VERSION     DBMDTI010000215.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6274_length_3427_cov_2.069453"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            120..1319
                     /locus_tag="ACZPB6_08620"
     CDS             120..1319
                     /locus_tag="ACZPB6_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184567.1"
                     /translation="MKSASVLSVLAAGTIAAISTSVCAGSNGTDAVMRSLDQVNRIYT
                     PEKTTHTELLLRLNDLGCHYRVNNRMKADPEDVMKHTFGWFYRLLPDERGIANCFPVT
                     VFFQNREDGRDVIEIDGGSGGDRKALINALKRRWGEPLTTKGRRQNAHYWVFNAGDRT
                     IVLKEMGILRNYEIEILPVSYYDAVKRRIEEEQAKAEAAKQAELAAAQLVASNLKAAE
                     KEAREREERIARAREIARTHAGRLFGILTPGETDFLVAQAEAEAAGCRIERDGSLVKT
                     GEVRGEPCFILPGHPALSVEQFKGGLGFMVRYGADGNGVDAMRMKLSALGPVSSYENR
                     GSINHYWQDAGMTVVETGHERHGSPVSFLFISKATFEKELERILQEKAEAEAEHRREN
                     ETLQKMF"
     gene            complement(1388..2431)
                     /locus_tag="ACZPB6_08625"
     CDS             complement(1388..2431)
                     /locus_tag="ACZPB6_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1184568.1"
                     /translation="MSIVFRPLIMLASSIVRHRPVRDYSCCVLDSAFCFRDSSLPAPV
                     VMPSRRDFFNPAEAYVMTLGDGQSKKRVIQLLNQISRFFGAADLHAMEWQMLTYDHVL
                     AFRETRVRSGLSPATINLQLSVIKMTCKQCWLKNLMPLQTYAAIKEVKSVRGGRVPKG
                     RALKPAESGRLLKTTEADGDIRSKRDAAIIALALGCGLRRSEIASLLLEQVDHVRHTI
                     TVRGKGNKERRVAVADAVWKRLAVWLEARAGDPCPNVFLAVRRYGRISTGHSITSSAV
                     YQILKTRSSQSGVEDFSPHDLRRTFATRLLGAGTDINLVRKAMGHASVLTTQRYDKRE
                     DEEVEEATRKILI"
     gene            2655..3137
                     /locus_tag="ACZPB6_08630"
     CDS             2655..3137
                     /locus_tag="ACZPB6_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012920.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HIR1184569.1"
                     /translation="MPALESRSAVLESCRWDELEPLGFAPAESVAAITCVELRVPCGS
                     PSPIGEGSVGKVDLNRMLVKNPLATFLVRCTGTAMTAAGIEFGDVCIVDRSLDARSGD
                     IVLAYINARFTMRRYEEYGEDGRRLVPVAKEGVHPVTEITDKDDFNIEGVVTGLCRKF
                     "
BASE COUNT          659 a         1053 c         1121 g          594 t
ORIGIN      
        1 tgagacggcg cccttcatga gcacaggctg ttacaaccgg acatgagtcg gacagtcgct
       61 tttcctataa ttttcagaat gccttcaatc ggaaaacatt cctgagaaac aacccgctca
      121 tgaaaagcgc atccgtctta tccgtcctcg cggccggcac gattgccgcg atttccactt
      181 cggtctgcgc aggctcgaac ggcaccgatg ccgtcatgcg cagcctcgac caggtcaacc
      241 gcatctacac gcccgaaaag acgacgcaca cggagcttct cctgcgtctc aacgacctgg
      301 gttgccacta ccgcgtgaac aaccgcatga aggcggaccc cgaggacgtc atgaagcaca
      361 ccttcgggtg gttctacagg cttctgcccg acgagcgcgg catcgccaac tgcttccccg
      421 tcacggtctt cttccagaac agggaggacg gtcgcgacgt gatcgagatc gacggcggct
      481 cgggcggcga ccgcaaggcg ctcatcaatg cgctcaagcg acgctggggc gagcccctca
      541 cgacgaaggg ccgcaggcag aacgcccact actgggtctt caacgcaggc gaccgaacca
      601 tcgtcctcaa ggagatgggc atcctgcgca actacgagat cgagatcctg cccgtctcct
      661 actatgacgc cgtcaagcgc cgcatcgagg aggagcaggc gaaggccgag gccgcgaagc
      721 aggccgagct ggccgccgcc caacttgtgg cctcgaacct caaggccgcc gagaaggagg
      781 cccgtgagcg cgaggagcgc atcgcacgcg cccgtgagat cgcccgcacc catgccggac
      841 ggctctttgg catcctgacg ccgggtgaaa ccgactttct cgtcgctcag gccgaggccg
      901 aggccgcagg ctgcaggatc gagcgcgacg gttcgctcgt gaagacgggc gaggtacgcg
      961 gcgagccgtg cttcattctg ccaggccatc ccgcactttc cgtcgagcag ttcaagggcg
     1021 gactcggctt catggtccgc tacggcgcgg acggcaacgg cgtcgacgcc atgcgcatga
     1081 agctttcggc cctcggcccc gtgtcgagct acgaaaaccg cggctccatc aaccactact
     1141 ggcaggacgc gggcatgacc gtcgttgaga cgggccacga gcgccacggc tctcccgtga
     1201 gcttcctctt catctccaag gccaccttcg agaaggagct cgagcgcatc ctgcaggaaa
     1261 aggccgaggc cgaagccgaa cacaggcgtg agaacgaaac gctgcagaag atgttctgac
     1321 gtgttccggc gcctttcgca gttttcgaaa cggtcctccc gaacttgggc aggaccgttt
     1381 ggttgggtca aatgaggatt tttctcgtcg cctcctcgac ctcctcgtcc tcgcgcttgt
     1441 cgtagcgctg ggtggtgagg acggaggcgt ggcccatggc ctttcgcacg aggttgatgt
     1501 cggtgcctgc accgagaagc ctcgtcgcga aggttcgtct gaggtcgtga ggtgagaagt
     1561 cctcgacgcc cgactggctg gagcgggtct tgaggatctg gtagacggcg gaggaggtga
     1621 tggagtggcc cgtcgagatc ctgccgtagc ggcgcaccgc caggaagacg ttgggacagg
     1681 gatcgcccgc cctggcctcg agccagacgg cgaggcgctt ccatacggcg tcggccaccg
     1741 cgacgcggcg ctccttgttg cccttgccgc ggaccgtgat cgtgtggcgc acgtggtcga
     1801 cctgctcaag aagaagagag gcgatttcgg atcggcgaag cccgcagccg agcgcgagcg
     1861 cgatgatggc ggcgtcgcgc ttcgagcgga tgtcgccgtc ggcctcggtg gtcttgagaa
     1921 gacgtcccga ctcggcgggc ttgagggcgc ggcccttcgg tacgcggccg ccgcgcacgg
     1981 atttcacctc cttgatggct gcataggtct gcagcggcat gaggttcttg agccagcact
     2041 gcttgcaggt catcttgatg acggagagct gcaggttgat ggttgcgggc gagaggcccg
     2101 agcgcacgcg cgtctcccgg aaggcaagta catggtcgta ggtcagcatc tgccactcca
     2161 tggcgtgaag gtcggcggcg ccgaagaagc gcgagatctg gttgagaagc tggatgacgc
     2221 gcttcttcga ctgaccgtcg ccgagcgtca tgacgtaggc ctcggcggga ttgaagaagt
     2281 cgcggcggct cggcatgacg acgggcgcgg gaagagagga gtccctgaag cagaaggcgc
     2341 tgtcaaggac gcagcaggag tagtccctga cgggacgatg acggacgatg gatgaagcca
     2401 gcatgatgag cggacgaaaa acaatggaca cgaacgatgg gcgaaccctg aaaaaggcgt
     2461 ggttccgcaa gcttgtgcgc ggaattcacg cgttgtcgca aaaactataa acgtttttca
     2521 ggaaacactc gtccccgcgt tttgaggctg gatcggtgac gggtgaacgt gaggaacatc
     2581 ggtttggctg aggcaggcta tgattcagga tatcctgatc accacgtcgc ctcttcaaaa
     2641 gaaggattcc cgtcatgccc gctcttgagt cccgatctgc cgtccttgag tcctgtcgct
     2701 gggacgagct cgagccgctc ggctttgcgc ccgccgagag cgtggcggcg atcacctgcg
     2761 tcgagctccg agtgccctgt gggtctccgt ctccgatcgg ggagggctcg gtggggaagg
     2821 ttgacctcaa ccgcatgctc gtgaagaatc cgctcgccac ctttctcgtg aggtgcacgg
     2881 gcacggccat gacggccgcg gggatcgagt tcggggacgt gtgcatcgtc gaccgttcgc
     2941 ttgacgccag gtcgggcgac atcgtgctcg cctacatcaa cgcccgcttc acgatgcgcc
     3001 gctatgagga atacggagaa gacggacgcc gcctcgtgcc cgtcgccaag gaaggcgttc
     3061 atcccgtcac cgagatcacc gacaaggacg acttcaacat cgagggcgtc gtgacggggc
     3121 tctgccggaa gttctgactg agtgcacggc cgtgaaaacc ctcgtgtgtc ggcgtgacga
     3181 cactcagacg gccttgtctc ctgcagactg aacgaagaag agacccgcgg accgggcggg
     3241 tcccgaccat gaacaggaga ccagaatgca caagtttctt cgcacgatga cggcagccgc
     3301 cgtcgcagtc gccatggcgc ccgccttcgc cgccgatccc acctacaacg tcgacgttgt
     3361 cgtcgtgggc gcgggtgcag cgggcaccgc cgcaggctgg gccgctgccg aaaagggcct
     3421 caaggtc
//
LOCUS       DBMDTI010000216         3419 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6292_length_3419_cov_2.501206, whole genome shotgun sequence.
ACCESSION   DBMDTI010000216 DBMDTI010000000
VERSION     DBMDTI010000216.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6292_length_3419_cov_2.501206"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            197..847
                     /locus_tag="ACZPB6_08635"
     CDS             197..847
                     /locus_tag="ACZPB6_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1184570.1"
                     /translation="MNMLAALFAGAALLAGAVLAGYRMRTRGPAAAGKSVFLAGFAGV
                     LLVSSLGVYATLGRWSDWDKAEVDHDVDYLLAAKLTDARRLVQMTPDSAYAQQQLALV
                     SLEVGRYEDAVSAIDRSISIAGETPELLGIKVFAQYYRDGRQFAPETQAAVGRVFDMN
                     PLEVRTRMLLGQDAFLNGRYAEAVGHWRMLLDAGAAPDKERALKTAIAKAEARMTR"
     gene            903..1586
                     /locus_tag="ACZPB6_08640"
     CDS             903..1586
                     /locus_tag="ACZPB6_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1184571.1"
                     /translation="MSEKIDRRKFLAGAGAGSLVLVSLGGCGSSQVRQPGTPHYGMVF
                     DQNKCVGCRDCRIACNETNKLPKGVTRLLLELQDDKIEGKRGYAADRRYVRVSCQQCS
                     DAPCVTVCPTGAAHRDPKTGIVTMYADKCVGCKYCIVACPYNARFINEETKVADNCDF
                     CLHTRLEKGQQPACVEACKYDALVFGDLNDPNSYINKLLAVKDSVRIRPELGTEPSLR
                     YIPIVKQGV"
     gene            1589..2521
                     /gene="nrfD"
                     /locus_tag="ACZPB6_08645"
     CDS             1589..2521
                     /gene="nrfD"
                     /locus_tag="ACZPB6_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR1184572.1"
                     /translation="MDIAMNFTLGLSQGIAWPWPIAVYLFLAGISGGAVAVALMLNRY
                     RNQVAENTPVLKAATLVGFVTIVLGMICLVADLTNPLVFWRILIYYNLTSVMSLGVIA
                     LLFYIPLVFVLMLVAFRKEVKGCGALAWLDGIIGAFDGIRGALQWIVLILAITICAYT
                     GFLISALIRFPLINTAVLPALFIASGLSAGAAAAKLVAVGCFGEEEHSADMKLLHGAE
                     WPIMAAEALCIFMIAVSLISGNAAAQAAVEAFVSGTWSTVFWVGVVGVGFVLPVLLNF
                     VKSTASFYLAGLCSVVGMLCLRHFILYAGQIFGL"
     gene            2757..3269
                     /locus_tag="ACZPB6_08650"
     CDS             2757..3269
                     /locus_tag="ACZPB6_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HIR1184573.1"
                     /translation="MTMQKKMLKTAAAACAAALAAVLLAGCGDEASKKTYSPVPFTKH
                     DRCHLCGMVLAHYEGPKAQIIIKGAEDTPLMFCSGRDAFTYALQPENQRRLLAFFIHD
                     LGKTSWETPADDALVPAASAMYVYGHGREGVMGDEPVAFTEKSRADAFIAKWGGQVYK
                     YEEITLKRLD"
BASE COUNT          628 a         1134 c          997 g          660 t
ORIGIN      
        1 catgacggcg caggtcagat tcctcccgaa tccgccctcg tcttcgacct ccagctcgtc
       61 aaggtcgaaa aggttggtgc tcatcagcag agcttcggca tgcccggcat gtccggcggc
      121 atgtccaagg aaatgatgga gaagatgaat ccccacaagt gaggatgatt cttcgcaatc
      181 gcatggagaa cgcgtgatga acatgctcgc agccctcttc gcaggcgccg ccctcctcgc
      241 aggtgcggtt cttgcaggct accggatgcg gacccgtggt ccggccgccg caggcaagag
      301 cgtgttcctc gccggtttcg caggcgtgct gctcgtctca agcctcggcg tctatgcaac
      361 gcttggacgc tggagcgact gggacaaggc agaggtcgac cacgacgtcg actatctgct
      421 cgccgcgaag ctgacggatg cgcgccgtct ggtccaaatg acccccgaca gcgcatacgc
      481 tcagcagcag ctcgcactgg tctcgctcga agtgggccgg tacgaggatg cagtctcggc
      541 gattgaccgc agcatcagca tcgcgggaga aactcccgag ctgctgggca tcaaggtctt
      601 cgcccagtac tatcgcgacg gcaggcagtt cgcccccgag acgcaggccg ccgtcgggcg
      661 ggtgttcgac atgaaccccc ttgaggtccg caccaggatg ctcctcggtc aggacgcctt
      721 cctcaacggc agatacgccg aagcggttgg ccattggcgg atgcttctgg atgcgggagc
      781 ggctcccgac aaggagcgcg ccctcaagac tgcgattgcc aaagccgaag ccaggatgac
      841 tcggtaagca atttaaccga cagattctcc taaccccggg aggtctgtca ttggagaaca
      901 tcgtgagcga aaagatcgac aggagaaaat ttctcgctgg cgccggtgct ggctccctgg
      961 tcctcgtgtc cctcggcgga tgcggctcca gccaggtccg tcagcccggt acgcctcact
     1021 atggtatggt cttcgaccag aacaagtgcg ttggctgccg cgactgcaga atcgcctgca
     1081 acgaaacgaa caagcttccc aagggagtga ctcgtctgtt gctcgagttg caggacgaca
     1141 aaatcgaagg caagcgcggc tatgccgccg accgccgcta cgtgcgcgtg tcctgccagc
     1201 agtgctctga cgccccgtgc gtcacggtct gcccgacggg cgccgcccat cgcgacccga
     1261 agaccggcat cgtgacgatg tacgccgaca agtgcgtcgg ctgcaagtac tgcatcgtcg
     1321 cctgcccgta caacgcgcgt ttcatcaatg aggagacgaa ggtcgccgac aactgcgact
     1381 tctgcctgca cacgcgactc gaaaagggtc agcagcctgc atgcgtggaa gcctgcaagt
     1441 acgacgccct cgtgttcggt gacctcaacg atccgaactc gtacatcaac aagctgctcg
     1501 ccgtcaagga ttccgtgcgc atccgcccgg aactcggcac ggagcccagc ctgcgttaca
     1561 tccccattgt taagcaagga gtgtaaagat ggacatcgcc atgaacttta cacttggtct
     1621 gagccaaggg atcgcctggc cgtggccgat cgccgtgtac ctgttcctcg caggcatctc
     1681 gggcggcgcc gtggctgtgg cgctcatgct taaccgctac cgcaaccagg ttgcggagaa
     1741 cacgccggtt ctcaaggccg cgacgctcgt cggctttgtc accatcgtgc tcggcatgat
     1801 ctgcctcgtg gccgacttga cgaatccgct ggtattctgg cgcattctca tctactacaa
     1861 cctgacctcc gtgatgagcc tcggtgttat agcgcttctg ttctatatcc cgctcgtctt
     1921 cgtgctcatg ctcgtcgcct tccgcaagga agtgaagggc tgcggcgcgc tggcctggct
     1981 cgacggcatc atcggcgcct ttgacggcat ccgcggtgcg cttcagtgga tcgtcctgat
     2041 tctcgcaatc acgatctgcg cgtacaccgg cttcctgatc tccgccctga tccgcttccc
     2101 gctcatcaac acggctgtgc tcccggcgct attcatcgcc tcgggtctgt ccgccggtgc
     2161 cgctgctgcc aagctcgtcg ccgtcggctg cttcggcgaa gaagaacaca gtgccgacat
     2221 gaagctcctg cacggcgccg aatggccgat catggccgcc gaagcgctct gcatcttcat
     2281 gatcgcggtc tccctgatct ccggcaacgc cgctgctcag gctgccgtcg aggcctttgt
     2341 ctcgggtacc tggtccaccg tgttctgggt cggtgtcgtc ggcgtcggct tcgtgctgcc
     2401 cgtgctgctg aacttcgtca agagcaccgc gagcttctac ctcgccggtc tctgcagcgt
     2461 cgtcggcatg ctctgcctgc gtcacttcat cctgtacgcc ggtcagatct tcggtctcta
     2521 atgtccctcg cggtcatctg aaccgaagcg tgtgaaagca cgcgaccaat caaccatccg
     2581 ccccgccggc aacggcgggg cagatggctg agaaaaaaac gagaattccc ccgagcccgt
     2641 caacgggccg tttttttatc aggagacccg tccgcatttt cgaacgcatc gttcgagaac
     2701 gtcgtacgat tggatttttc cccgaagctt tccgccaacc atatgaggag cgtgcaatga
     2761 cgatgcagaa gaagatgttg aagaccgccg cggccgcctg cgctgcggct ctcgcggccg
     2821 tcctgcttgc aggatgcggc gatgaggcct cgaagaagac ctactcgccc gtccccttca
     2881 ccaagcacga ccgctgccat ctctgcggca tggtccttgc acactacgag ggccccaagg
     2941 cccagatcat catcaagggc gcagaagaca caccgctcat gttctgcagc ggtcgcgacg
     3001 ccttcaccta cgcgctccag cccgagaacc agcgcaggct tctcgccttc ttcatccacg
     3061 acctcggcaa gacctcctgg gagacgcccg ccgacgacgc gctcgtgccc gcggcttccg
     3121 ccatgtacgt ctacggccac ggccgcgagg gcgtgatggg ggacgaaccc gtcgccttca
     3181 ccgaaaagtc cagggccgac gccttcatcg caaagtgggg cggccaggtc tacaagtacg
     3241 aggaaatcac cctcaagcgc cttgactgag gaagcccggc atgaacgcat ttgcgcggct
     3301 tctcctttgc acggccctct cgggcctcgc cctcggggcg caggcgcgca ccttcgaggt
     3361 ctccgacttc gccgagcttg acgacgccgt cctcggcctc gatgacggcg acgagatcg
//
LOCUS       DBMDTI010000217         3417 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6296_length_3417_cov_2.336852, whole genome shotgun sequence.
ACCESSION   DBMDTI010000217 DBMDTI010000000
VERSION     DBMDTI010000217.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6296_length_3417_cov_2.336852"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="ACZPB6_08655"
     CDS             <1..322
                     /locus_tag="ACZPB6_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184574.1"
                     /translation="HATASDKQVLVQWAYVTLSKTSAAKQIQTIPSAKVKNIESSAQK
                     ALTNLVVGRCSSPAVKVLMKNPKNGLQETMTLLAGKLVNAEIERRASPILSLTMTDLM
                     GLRK"
     gene            448..1116
                     /locus_tag="ACZPB6_08660"
     CDS             448..1116
                     /locus_tag="ACZPB6_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184575.1"
                     /translation="MRKLCLVPALLLAAASASAETPVKLWNDYVYNQPVESVKAEAGI
                     FDCSQRGQQMFCRANVEFAGHKDWGEVFFFTNGRLKAVSLFAPISRAHFTDAAAAVRK
                     TGMTPAVLTDHEDKVAFDFFQAKLQAKSMASIDRELATKETELLKGKKVGYVFIDAKT
                     LLESVKSGNVTNAAQFFQIHAPRTLRTTEILLDEQGVAVSFRAPMAVQDETGKSMLEE
                     KKSK"
     gene            complement(1208..1846)
                     /gene="hisIE"
                     /locus_tag="ACZPB6_08665"
     CDS             complement(1208..1846)
                     /gene="hisIE"
                     /locus_tag="ACZPB6_08665"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIR1184576.1"
                     /translation="MLTNEDIKALDWEKTGGLIPAVVQDACSGRVLMLGCMNPEALEK
                     TLETKKVTFWSRTKGRLWTKGETSGNWLHLVDIGTDCDSDALLVLVNPDGPTCHRGTP
                     SCFSPVEHKWNFLRDLEVLLESRRNADPATSYTASLYARGTKRIAQKVGEEGVETALA
                     ATVHDREELRNEAADLVYHLLVLLADADLELADVIDVLRARHASREKAAEAK"
     gene            complement(1840..2616)
                     /gene="hisF"
                     /locus_tag="ACZPB6_08670"
     CDS             complement(1840..2616)
                     /gene="hisF"
                     /locus_tag="ACZPB6_08670"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR1184577.1"
                     /translation="MLAKRIIPCLDVKDGVVVKGVKFRGHEVVGEIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWISRVAEAIDIPFCVAGGIRTVEDAGRLLAYGADKISV
                     NSPALADPDLISRLADRFGVQCVVVGIDSRHEEGSDEYRVNQFTGDEKKTRVTQWSTL
                     DWVEEVQKRGAGEIVLNMMNQDGVRRGYDLRQLAAVRSVCRVPLIASGGAGEPVHFLE
                     AFRDAKVDGALAASVFHKKIINIGDLKNYLRENNIEVRTC"
     gene            complement(2598..3335)
                     /gene="hisA"
                     /locus_tag="ACZPB6_08675"
     CDS             complement(2598..3335)
                     /gene="hisA"
                     /locus_tag="ACZPB6_08675"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR1184578.1"
                     /translation="MIIPAIDLIDGRVVRLHQGDYGAERSYGDDPLDRLQAYEAAGAK
                     LLHIVDLTGAKDPAKRQIPLLRHLLSKLFVPVQTGGGVRSEEDVRALLDAGATRVVVG
                     STAVRCTEEVLRWMKTFGAERIVLALDVRIDAAGRRNIAVSGWQEDSGVVIEDLIRVF
                     EPAGLRHVLTTDISRDGTLSGSNAALYADLSKAFPAIGFQASGGIGGLDDIRAVSTTG
                     ASGVIVGRALLEGRFTVEEAIECWPNA"
BASE COUNT          591 a         1148 c         1090 g          588 t
ORIGIN      
        1 gcacgccacc gcgagcgaca agcaggtcct cgtgcagtgg gcctacgtca cgctctcgaa
       61 gacctccgcc gccaagcaga tccagacgat tccgtcggcc aaggtgaaga acatcgagtc
      121 gagcgcccag aaggcgctga cgaacctcgt cgtcggccgc tgctcctcgc ccgccgtcaa
      181 ggtcctcatg aagaatccca agaacggcct gcaggagacc atgacgctgc tcgcgggcaa
      241 gctcgtcaac gccgaaatcg agcgccgcgc ctcgcccatc ctctcgctca cgatgaccga
      301 cctgatgggc ctcaggaagt aggccccggt cccgcacatc gagaaaaacg gcgaaaagtc
      361 cgtcctcccg gcgggctttt ccgcattgac gaacgggcgt cgacgcccga tacttgatta
      421 tttccgccca atctctggag atcccgcatg cgcaagctct gcctcgttcc cgctcttctt
      481 ctggccgccg ccagcgcctc ggctgaaacg ccggtcaagc tctggaacga ctacgtctac
      541 aaccagcccg tcgagagcgt gaaggccgaa gccgggatct tcgactgctc gcagcgcggc
      601 cagcagatgt tctgccgcgc caacgtcgaa tttgcgggcc acaaggactg gggcgaggtg
      661 ttcttcttca cgaacggccg cctcaaggcc gtctcgctct tcgcgccgat ctcccgcgcc
      721 cacttcaccg atgcggccgc cgccgtgcgc aagacgggca tgacgcccgc cgtcctcacg
      781 gaccacgagg acaaggtcgc cttcgacttc ttccaggcca agcttcaggc gaagtccatg
      841 gcctccatcg accgcgagct cgccacgaag gaaaccgagc ttctcaaggg caagaaggtg
      901 ggctacgtct tcatcgacgc caagacgctc cttgaaagcg tcaagagcgg caacgtcacc
      961 aatgccgccc agttcttcca gatccacgcc ccgcgcaccc tgcgcaccac cgagatcctg
     1021 ctcgacgaac agggcgttgc cgtgagcttc cgcgccccga tggccgtcca ggacgagacg
     1081 ggcaagagca tgctcgaaga aaagaagtcc aaataagccc gactttccgg cacacgcgac
     1141 aagaaaggcc ggtctccgct gcggaggacc ggcctttctt catttttcag agtctgcctc
     1201 ggaactctta cttcgcctcg gcggcctttt cgcggctcgc gtggcgggcg cgcagcacgt
     1261 cgatcacgtc ggcgagctcg aggtccgcgt cggcgaggag cacgagcagg tgatagacga
     1321 ggtcggcggc ctcgttcctg agctcctccc tgtcgtgcac ggtagcggcg agcgccgtct
     1381 cgacgccctc ttcgcccacc ttctgcgcga tgcgcttggt gccgcgggcg tagaggctgg
     1441 cggtgtagga cgtggccgga tcggcgttgc ggcggctctc taggagcacc tccagatcgc
     1501 gcaggaagtt ccacttgtgc tcgacgggcg agaagcagct cggcgtgcca cggtggcagg
     1561 tcgggccgtc gggattgacg agcacgagga gcgcgtcgct gtcgcagtcc gtgccgatgt
     1621 caacgaggtg cagccagttg ccgctcgtct cgcccttggt ccagagacgg cccttggtgc
     1681 gggaccagaa cgtgaccttc ttcgtctcga gggtcttttc aagcgcctcg gggttcatgc
     1741 agccgagcat gagcacccgg cccgagcagg cgtcctgcac gacggccgga atcagtccgc
     1801 cggtcttctc ccagtcgagc gccttgatgt cttcgttggt cagcatgttc tcacctcaat
     1861 gttgttctcg cggagatagt tcttcaggtc tccgatgttg atgatcttct tgtggaagac
     1921 ggaggcggca agcgctccgt cgaccttcgc gtctcggaag gcctcgagga agtggacggg
     1981 ctcgcccgcg ccgccgctcg cgatgagcgg cacgcggcag accgagcgca cggcggcgag
     2041 ctgcctgagg tcgtagccgc ggcgcacgcc gtcctggttc atcatgttga gcacgatctc
     2101 gcccgcgccg cgcttctgca cctcctcgac ccagtcgagg gtgctccact gcgtgacgcg
     2161 cgtcttcttt tcgtcgcccg tgaactggtt gacgcggtat tcgtccgaac cttcctcatg
     2221 acgggagtcg atcccgacca cgacgcactg cacgccgaag cggtccgcaa gacgggagat
     2281 gaggtccggg tcggcgagcg ccggcgagtt gacggagatc ttgtcggcgc cgtaggcgag
     2341 aagcctgccg gcgtcctcga ccgtgcggat gccgcccgcg acgcagaacg ggatgtcgat
     2401 cgcctcggcg acgcggctga tccagctctt gtcgacgacg cggccgtcgg acgaggcggt
     2461 gatgtcgtag aagacgagct cgtcggcgcc ctcttcggca tagcgctttg cgagcggcac
     2521 gatttcgccc acgacctcgt ggccgcggaa cttgacgccc ttgacgacga cgccgtcctt
     2581 cacgtcaaga caggggatta tgcgtttggc cagcattcga tagcctcttc aacggtaaag
     2641 cgtccctcga gaagcgcgcg gccgacgatg acgccggagg cgcccgtcgt actgaccgca
     2701 cggatgtcgt ccagcccgcc gatgccgccc gaggcctgga agccgatggc ggggaaggcc
     2761 ttcgaaaggt ccgcgtagag cgcggcgttg gaacccgaga gggtgccgtc gcgcgagatg
     2821 tcggtggtga gcacgtggcg caggcccgca ggctcgaaga cgcggatcag atcctcgatc
     2881 acgacgcccg agtcctcctg ccagccgctc acggcgatgt tgcggcggcc tgccgcgtcg
     2941 atgcgcacgt cgagcgcgag cacgatgcgc tcggcgccga aggttttcat ccagcgaaga
     3001 acctcctccg tgcacctcac ggcggtggag cccactacga cgcgggtggc gcccgcatca
     3061 agaagagccc tcacatcctc ctcgctcctg acgccaccgc ccgtctgcac aggcacgaag
     3121 agcttcgaaa gaaggtgcct gagaagcggg atctggcgct tggccggatc cttcgcaccc
     3181 gtcagatcca cgatgtgcag gagcttcgcc cctgcggcct cgtaggcctg aaggcgatcg
     3241 aggggatcgt cgccgtagct tcgctcggcg ccgtagtcgc cctggtgcag ccgcacgacg
     3301 cggccgtcga tgaggtcgat ggcggggatg atcatgcttc gattcccagg aagtttgcaa
     3361 tcaggcgtgc gccggcgcgg ccggagcgtt ccggatggaa ctgcacgccc atgaagt
//
LOCUS       DBMDTI010000218         3407 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6313_length_3407_cov_3.422565, whole genome shotgun sequence.
ACCESSION   DBMDTI010000218 DBMDTI010000000
VERSION     DBMDTI010000218.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6313_length_3407_cov_3.422565"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="ACZPB6_08680"
     CDS             complement(<1..690)
                     /locus_tag="ACZPB6_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR1184579.1"
                     /translation="MSELKPGLPERLERVRGELAAAERAAGRPAGSVRLIAVGKTFPV
                     EACEEAYACGQRAFGENYVQEGVEKIVHFRAVHPEDPGEWHFIGPLQANKTRLVVEHF
                     DWVQSVDRLRIASRLSAQRPAGMPPLNILIEVNIDGEETKSGVAPSEVEAFADEIAGY
                     PGLRLRGLMAIPAPAADSESRRRPLAAMRALFERLRVKHPGMDTLSMGMSADMDEAVS
                     EGATMVRVGRAV"
     gene            1172..1879
                     /locus_tag="ACZPB6_08685"
     CDS             1172..1879
                     /locus_tag="ACZPB6_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ImuA family protein"
                     /protein_id="HIR1184580.1"
                     /translation="MSTAAVLSDFFTAALREARSERPASLASRIPALDELTEGGFPVN
                     ALTELFIPHAGIGEVRMLLPALASLKHVTWVLPCDSEAAPYAPALADAGLDLDRQLFS
                     IPSTPEEAFWCAEQAAASGETSAVVVWLNPLVHGKDGAALARLDMAAHSTGTTIFLIR
                     PSSMACTPSPAELRLMLRPGRSGTVHVRATRRGAFFDRTREADVSLEERPLVPAPRAC
                     RRKVQQALASARLSPAC"
     gene            1918..2655
                     /locus_tag="ACZPB6_08690"
     CDS             1918..2655
                     /locus_tag="ACZPB6_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184581.1"
                     /translation="MNIATCAKTSLKAVVSVPVGIRLQELPSMVKSLCAGIAGNADCS
                     GGELTLTLEGLDETRRIVVSVNNGSARAFTDVLTDRLIRTPIPGSLRRMTLSLEPEIS
                     DLIRDTPNASVPTGLAARLQARLGPGRVFRLSNLDEGLPSTSSRLAPVADAFARKPMS
                     APAGKADVRPTMLLSDPLPLHASDDGLAWRQESLRLVDGPVRHADDSEIRDYFVAESS
                     SGRRVWLYRTEDSDKTEVRWHLHGFFS"
     gene            2701..3321
                     /locus_tag="ACZPB6_08695"
     CDS             2701..3321
                     /locus_tag="ACZPB6_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIR1184582.1"
                     /translation="MKEPILLHSCCAPCSSAILEWLLQNDYAPHVYYFNPNIFPEEEY
                     LVRKNECIRYCEKLAVPFTDGDWDHAGWRNAVKGHECDPERGERCRLCFGVRMRATAA
                     MAAKLGIRRFTTTLAGSRWKRLDQIREAALEAAALHPGTTYWDQNWRKGGLQERRGEI
                     LREENFYNQLWCGCEFSMGHLRERDPSDLPAHMKPYLEALGLTGKG"
BASE COUNT          666 a         1169 c          930 g          642 t
ORIGIN      
        1 gacggccctg ccgaccctca ccatggtcgc tccctccgac accgcctcgt ccatgtcggc
       61 gctcatgccc atggaaagcg tgtccatgcc ggggtgcttc acacggagcc gctcgaagag
      121 tgcgcgcatg gccgcaaggg gccttcgacg agattcgctg tccgccgccg gcgcgggaat
      181 cgccatcagc ccccttagcc tcaggcccgg atagccggcg atctcgtccg cgaaggcctc
      241 gacttcggaa ggcgcaaccc ccgacttcgt ctcctctccg tcgatgttga cctcgatcag
      301 aatgttgagg ggcggcatgc cagcgggtct ttgagcggaa agccgcgacg caatgcgaag
      361 gcggtcgacg gactgaaccc agtcgaagtg ctcgacgacg aggcgggttt tgttggcctg
      421 aagcggcccg atgaagtgcc actcgccggg atcctccgga tgcaccgcac gaaagtgcac
      481 gatcttctcc accccttcct gaacgtagtt ttccccgaac gccctctgac cgcaggcata
      541 ggcctcctcg caggcctcga cgggaaaggt cttcccgacg gcaatgagcc tgacggagcc
      601 cgcaggcctg cccgccgcac gctccgcggc cgcaagttca ccccgcaccc gttccagtcg
      661 ttcgggcagt cccggcttga gttcgctcac gatgcattct cctgaaagct gttcgaagga
      721 aaggcgtcct tcgtgcgcaa caacagtcta gcaagggtgg cggcgcatcg gcatcgcacg
      781 aagattgacg atttcttgac ctccgtcaaa ttttcaaatg catcattttt cggatttgat
      841 cctctcgaag cccccctccg aacagccgtc cccgccgttc tgacgcaaac gcagctcacg
      901 ctgtcttttt cgcaaactga agcccggagc ggatttgcga aggcgcacaa cttgcgacac
      961 actgcatcct ttcatgatcc tgcaaagaca cgcaatcaac caccaaaaca aatcttttac
     1021 ttactcttca acaagatagc aagaataaaa tacaagtgga tcacgccaga ggatcacgag
     1081 tcgatacgca acagacagac tcaaagtaca ctgaccttac gacgacagca tcgttcaagt
     1141 gttacgttca agcaaattca ggagtaccag catgtcgacc gccgcagtcc tttccgactt
     1201 tttcaccgca gccctgagag aggctcgttc cgaacgccct gccagccttg caagccgcat
     1261 tcccgcgctc gacgaactga cggagggcgg ctttcccgtg aacgccctga ccgagctttt
     1321 cattccgcac gccggcatcg gagaggtccg catgctgctt cccgctctgg catccctgaa
     1381 gcacgtcacc tgggtgctgc cctgcgactc ggaggcggct ccctacgcgc ccgcccttgc
     1441 cgacgccggc ctcgatcttg accgacagct cttttccatt ccctctacgc ctgaagaggc
     1501 cttctggtgc gcggaacagg ccgccgccag cggagaaacc tcggccgtcg tcgtctggct
     1561 caatccgctc gttcacggga aggacggtgc ggccctcgca aggctcgaca tggccgcgca
     1621 ctcgacgggc acgacgatct tcctcatccg cccctcgagc atggcctgca cgccgtcccc
     1681 tgccgaactc cgtctgatgc tgcggccggg caggtccggc accgtacacg ttcgagctac
     1741 gcgacgcggc gcattcttcg accgcacgcg ggaagcagac gtcagtctgg aggaaaggcc
     1801 cctcgttccc gccccgcgcg catgccgccg caaagtgcaa caggctctcg cctcggcccg
     1861 tctctctccc gcctgctgaa cgcagccctc atgctcgtcc atcaaggagt ccagctcatg
     1921 aacatcgcca cctgcgccaa aacatccctc aaagccgtcg tcagcgtccc cgtcggcatc
     1981 cgtctgcagg aacttccctc aatggtcaaa agtctctgcg cagggattgc gggcaacgcc
     2041 gactgctcag gcggcgaact cactctcacg ctcgaggggc tggacgaaac ccgcaggatc
     2101 gtcgtcagcg tcaataacgg cagcgcacgc gccttcaccg acgtcctgac cgaccgactg
     2161 atccgcacgc ccattccggg aagcctgcga cgcatgacgc tctcgcttga gcccgaaatc
     2221 tctgatctca tcagggacac gccgaacgct tccgtcccga cggggcttgc cgcccgactt
     2281 caggcacgcc ttggtccggg acgcgtcttc cgtctcagca acctcgatga aggccttccc
     2341 tccacctcga gccgtcttgc tcccgttgcg gacgcctttg cgcgcaagcc tatgagcgct
     2401 cctgccggca aagcagacgt gaggcccacg atgcttctct ctgatccgct accgctccac
     2461 gcttcggacg atggacttgc atggcgtcaa gagtccctgc gtctggttga tggtcccgtg
     2521 cgccatgccg acgactcgga gatccgcgac tactttgtgg ccgaaagttc gagcggccgc
     2581 cgcgtctggc tttaccgaac ggaggattcc gacaagacgg aggtccgctg gcacctccac
     2641 gggttctttt cctgaaccta taatgagcgg acttcaatcc tcagcaaggg atccgagccc
     2701 gtgaaagaac ccattctgct gcacagctgc tgcgcgccct gttcctccgc cattctcgaa
     2761 tggcttctgc agaacgacta cgcgcctcac gtctactact tcaatcccaa catatttcct
     2821 gaagaggagt atctcgttcg caagaacgag tgcatccgct actgtgaaaa gcttgccgtt
     2881 ccgttcacgg acggcgactg ggaccacgca ggctggcgca acgccgtcaa gggacacgaa
     2941 tgcgatccag aacgcggcga acgctgcagg ctgtgtttcg gcgtgcgcat gagggccacc
     3001 gccgccatgg ccgcaaagct cggcatcagg cgctttacga caaccctcgc aggaagccgc
     3061 tggaagcgcc ttgatcagat tcgagaggcc gcgctcgaag cggctgccct gcaccccggc
     3121 accacctact gggatcagaa ctggcgcaag ggcggtctgc aggagcgcag gggcgagatc
     3181 ctcagagagg aaaacttcta caaccagctc tggtgcggct gcgaattctc catgggacat
     3241 cttcgagaac gcgatccctc cgaccttccc gcccacatga agccttatct tgaagcactg
     3301 gggcttacgg gaaagggctg acaagtttca atggacagcg ggcccgcagt cttctcgact
     3361 gcgggcccgc tgtcatttca atccgggtgt ttacttctcg ttgagaa
//
LOCUS       DBMDTI010000219         3407 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6316_length_3407_cov_2.032063, whole genome shotgun sequence.
ACCESSION   DBMDTI010000219 DBMDTI010000000
VERSION     DBMDTI010000219.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6316_length_3407_cov_2.032063"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="ACZPB6_08700"
     CDS             complement(<1..766)
                     /locus_tag="ACZPB6_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184583.1"
                     /translation="MKKILLAAAVSAAFGISGVQAAEDITVDVAVIGAGGAGLSAAVE
                     AANLGAKVVVVEKMAMAGGNTIRAAGGLNAPCTSLQAAKGVQDSLEIAYYDTMKGGHW
                     KNDPELVRTLVAGAGPSVEWLLALGGDLRDVGLLAGASKARAHRPTGGALVGPEVVRT
                     LYTAAKDRKIDMRMNTEATKILTDDGKVTGIQVKSKKDGTYTIHAKAVVNAAGGFGAN
                     NELVSKYVPRLKGFATTNHPGAQGEGIVLSEKIGAQL"
     gene            complement(1022..1258)
                     /locus_tag="ACZPB6_08705"
     CDS             complement(1022..1258)
                     /locus_tag="ACZPB6_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184584.1"
                     /translation="MSTALLVRREVAAFIPELSLVAALDGSIAGHVAGVRDVIHGRDG
                     RDRSVRQDPRAKGEALRHAKPEASSRDCGEGETC"
     gene            1398..2867
                     /locus_tag="ACZPB6_08710"
     CDS             1398..2867
                     /locus_tag="ACZPB6_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HIR1184585.1"
                     /translation="MKASISYPGFQRLRAAPLWKLLAAANAPAIIAILAEHLYEADHG
                     MRASEFYARVDRSLEELRATGVDMPSTAREYASQWLAQGLLERTLPYGSDEEVYSLTS
                     ASVDAVRFVTGMGRPRAEATESRLQLVITALDGLEEDTDADVERREHRLIEEQSRIRR
                     ELEAVGRGEVKVLDKDTALERTREILTLFSGLVGDFHRVRDSFEALNADLRRRIMECS
                     GSRGDVLEDVFAGLDLIRLSPAGRTFFAFWRLLNDPVESARFEEAIDAVLSRDFARDL
                     TVEERRMLRTMRRNLLERGGDVHDTTKRLAQGLRNFVESRQYLEEHRLNRLLTEAMRA
                     GIDAADEQSPVRRTGIELSRTGFTPRSAGQMVLWDPVTYAPPPPVEDGTPPPLDLSAV
                     GERIARSEINIRELRENVADVLSRKDQASVGEVLEAHPAKQGLGSVVGLMELALRHGI
                     PGSGTESLKWTGLDGRERSATVNQLFFVKDKIDELTRIY"
     gene            2845..>3407
                     /locus_tag="ACZPB6_08715"
     CDS             2845..>3407
                     /locus_tag="ACZPB6_08715"
                     /inference="COORDINATES: protein motif:HMM:NF025205.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HIR1184586.1"
                     /translation="MNSPESTDAAHNDEQAAGLWRGDAGGLKLDARRALVQLLKGPVL
                     DGMRMPVIWKALLESEDLIRSRLNDLFLDLVIDREQLFAFTRQADTDPVKAPILLKRK
                     PLNYIETYLFLFLRLRLSSAEGRGERAVIGLDEIREHLADIAGRNRDAVGFERHLAGA
                     VTKADHFGVLRRLKSGEDAFEISPVLK"
BASE COUNT          585 a         1089 c         1130 g          603 t
ORIGIN      
        1 cgagctgagc gccgatcttt tcggaaagga cgatgccttc accctgggcg cccggatggt
       61 tcgtcgtggc gaagcccttc aggcggggca cgtacttgga gacgagctcg ttgttggcgc
      121 cgaagccgcc ggccgcattg acgacggcct tggcgtggat cgtgtaggtg ccgtccttct
      181 tgctcttgac ctgaatgccc gtgaccttgc cgtcgtcggt gaggatcttc gtggcttcag
      241 tgttcatgcg catgtcgatc ttgcggtcct tggcggccgt gtagagcgtg cggacgactt
      301 cagggccgac gagggcgccg ccggtcggac gatgggcgcg ggccttggag gcgccggcga
      361 gcaggccgac gtcacgaagg tcgccgccga gagcgaggag ccattcgacg gagggacccg
      421 cacccgcgac gagcgtgcga acgagttcgg gatcgttctt ccagtgaccg cctttcatcg
      481 tgtcgtagta ggcgatttca agggagtcct gaacaccctt ggcggcctgg agcgacgtgc
      541 acggggcgtt gaggccgccg gctgcgcgga tcgtgttgcc gcccgccatg gccatctttt
      601 caacaacgac gaccttggcg ccgaggttgg ccgcttcgac ggcggcggaa aggcctgcgc
      661 cgcccgcgcc gatcacggcg acgtcgacgg tgatgtcttc agcggcctga acgccggaaa
      721 tgccgaaggc ggcggacacg gcggcggcaa gaagaatctt cttcattttg tgtgctctcc
      781 aatgaggttg cgccgcatgg agtgcaggca gtgcctgagc ccggtcgtgc ggcgcggttg
      841 gcaggcgcct gagttggtcg aaaatggcgc ccgatttaat gaatataact ccgatcggtc
      901 ggggacggaa tcgcgcgggt gcaggatggg gcaggcttcg ggctgccgac aaggtgaaat
      961 agtgttttcg tcaaacctgc gggacagccg gaatttccct gtgcaacgcc cgtttgattt
     1021 ctcagcaggt ctcgccctcg ccacaatctc gagaagatgc ctccggcttg gcgtgtcgca
     1081 gagcttctcc cttggctcga gggtcctgtc gaacgcttcg atctcgtcca tcgcgtccgt
     1141 ggatgacgtc ccgcacgccc gccacgtggc ctgcgatcga gccgtcgagg gcggcgacga
     1201 ggctcagttc tggaatgaag gccgcgactt cgcggcgcac gaggagtgct gtggacatgc
     1261 ggttcccagt ggaaaaatga gattgaagaa tgttgtgcgg cttcttccaa aaccgcgcgg
     1321 atcaaggctt tggagagggc ttctttgggt gcgcggggga ggaggaaagt cgcccgacca
     1381 tgtaaaatat acggcctatg aaagccagca tctcataccc cggcttccag cgtctgcgcg
     1441 cagcgcccct ctggaagctt ctggcggcag ccaatgcgcc cgccatcatc gccatcctgg
     1501 ccgagcacct ctacgaggcg gaccacggga tgcgtgcgtc cgaattttac gcgcgcgtcg
     1561 accgcagtct cgaggagctt cgcgcaacgg gcgtcgacat gccgtcgacg gcccgcgaat
     1621 acgcgtcgca gtggctcgcc caggggcttc tcgaacgcac gctgccctac ggcagcgacg
     1681 aggaggtcta ttcgctcacc tcggcctcgg tcgacgccgt gaggtttgtg accggcatgg
     1741 gcaggccccg tgcggaggcg acggaatccc gcctgcagct tgtgatcacc gcgctcgacg
     1801 gacttgaaga agacaccgac gcggacgtcg aacgccgcga gcatcgtctc atcgaggagc
     1861 agagccgcat ccgccgcgag cttgaggccg tgggcagggg cgaggtgaag gtgctggaca
     1921 aggacaccgc gctagagcgc acacgcgaga ttctcacgct cttctcgggg cttgtgggcg
     1981 actttcatcg cgtgcgcgac agcttcgagg cgctcaacgc tgaccttcgc cgccgcatca
     2041 tggagtgctc gggctcgagg ggcgacgtcc tcgaggacgt cttcgcaggg ctcgatctca
     2101 tcaggctcag ccctgcaggc cgaaccttct tcgcgttctg gcgcctgctc aacgatcccg
     2161 tcgagtccgc ccgcttcgag gaggcgatcg acgccgtgct ctcgcgcgac tttgcgcgcg
     2221 atcttaccgt cgaagagcgc aggatgctgc gcacgatgcg ccgcaacctg ctcgaaaggg
     2281 gcggggacgt gcacgacacg acgaagcgtc tggcccaggg tctcaggaac tttgtggaaa
     2341 gccgccagta tctcgaggaa caccgtctca acaggctcct caccgaggcg atgcgcgcag
     2401 ggatcgatgc ggccgacgag cagagtcccg tccgcaggac cgggatcgaa ctttcgagga
     2461 cgggcttcac accgaggtcc gcgggccaga tggtcctctg ggatcccgtc acctatgcgc
     2521 ctccgccgcc cgtcgaggac ggcacgccgc ctccgcttga cctctccgcc gtgggcgagc
     2581 gcattgcaag atccgaaatc aacatccgcg agctcaggga gaacgtcgcc gacgtgctct
     2641 cccgcaagga ccaggcgagt gtcggcgagg tactcgaggc gcatcccgcg aagcagggcc
     2701 tcggcagcgt cgtcggtctc atggagctcg cgcttcgtca cggcattccc ggctcgggca
     2761 ccgagagcct caagtggacg gggctcgacg gccgcgagcg ctccgccacc gtgaatcaac
     2821 tcttctttgt taaggacaag atcgatgaac tcacccgaat ctactgacgc cgcgcacaat
     2881 gacgaacagg ccgcgggcct ctggcgcggc gacgcgggcg ggttgaagct cgatgcaagg
     2941 cgcgcgctcg tgcagcttct caagggcccc gtgctcgacg gcatgcgcat gcccgtcatc
     3001 tggaaggccc tgcttgagag cgaggacctg atccgcagcc gcctcaacga cctctttctc
     3061 gacctcgtga tcgaccgcga gcagctcttc gcgttcacgc gccaggcgga caccgatccc
     3121 gtcaaggcgc cgatccttct gaagcgcaag ccgctcaact acatcgagac ctatcttttc
     3181 ctcttcctgc gcctgaggct ctcgagcgcc gagggcaggg gcgagcgcgc cgtcatcggc
     3241 cttgacgaaa tccgcgagca tctcgccgac atcgcgggcc gcaatcgcga cgccgtcggc
     3301 ttcgagaggc atctggcggg cgccgtcacc aaggcggacc acttcggcgt gctgcgccgc
     3361 ctcaagagcg gcgaggacgc cttcgagatc tcgcccgtcc tcaagat
//
LOCUS       DBMDTI010000220         3403 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6319_length_3403_cov_2.465173, whole genome shotgun sequence.
ACCESSION   DBMDTI010000220 DBMDTI010000000
VERSION     DBMDTI010000220.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6319_length_3403_cov_2.465173"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /gene="hisH"
                     /locus_tag="ACZPB6_08720"
     CDS             complement(<1..499)
                     /gene="hisH"
                     /locus_tag="ACZPB6_08720"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR1184587.1"
                     /translation="MRAVILDTGVANLSSLAFAVKRLGVEPIITREAAEIRAADRIFL
                     PGVGTARAAMAAIEERGLADLIREATQPVLGICLGEQILGRRSEETGGVDLLGLIDAD
                     VRALRADGLPLPHMGWNRVYPEDTPHARLLFRGIEAGDWFYFVHSYAMPTGACTTARC
                     TYGEEF"
     gene            complement(499..1572)
                     /gene="hisB"
                     /locus_tag="ACZPB6_08725"
     CDS             complement(499..1572)
                     /gene="hisB"
                     /locus_tag="ACZPB6_08725"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HIR1184588.1"
                     /translation="MNERILFVDRDGTILEEPEDFQVDRFEKMRFVEGVLPALKRLRE
                     AGWKLVMVSNQDGLGTKSFPRADFTGPHELMMQILESQGARFDEVLICPHMPGEGCEC
                     RKPGTGLVKKYLKDGVLDVANCYVIGDRETDMKLADNMGLRGLRVGPDGRSWAEIADE
                     IENAAKSARHARVERNTKETRIVVEAWLDRSGKNEINTGVGFFDHMLTQIAVHGGIRL
                     HVRAVGDLEIDDHHTIEDVGLALGEALKEALGDKRGIRRFGFLLPMDETLARCALDIS
                     GRPYLTFKAKFKHHKVGDMSTEMVEHFFRSLAQTMGVTLNLKAQGRNDHHVAEGLFKA
                     FGRTLRDAVKIEGSDLPSSKGVL"
     gene            complement(1569..2693)
                     /gene="hisC"
                     /locus_tag="ACZPB6_08730"
     CDS             complement(1569..2693)
                     /gene="hisC"
                     /locus_tag="ACZPB6_08730"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR1184589.1"
                     /translation="MAVFCERTAADPGLLARPDVQKLTPYQSARRIMQAAGVTGEVWL
                     NANESAEAEPMAPETKLFNRYPEPQPEAVVERYAAYAGVSPEQILVTRGGDEGIELLV
                     RTFCRPGEDAVLQFPPTYGMYAVSAETNGAEVVNLVTSPDRGWLPDVAEAAKVLEERT
                     DVKLVFACSPGNPTGTLIPVGMLRKLAELTAGRALLVIDEAYIEFTPANTAVELLKDY
                     PHVVIIRTLSKAFALAGLRCGFLLSSPAVIGMLRKVIAPYPIPVPCADIAAQALSPES
                     VSLMHRRAAQCVMRKCELEVALEDLDGVEAVYPSASNFLLVRFVDAARVFTALRDRGI
                     ILRDQSRQPTLENCIRITVGTKDENERLLTELEEILKEAA"
     gene            complement(2700..>3403)
                     /gene="hisD"
                     /locus_tag="ACZPB6_08735"
     CDS             complement(2700..>3403)
                     /gene="hisD"
                     /locus_tag="ACZPB6_08735"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR1184590.1"
                     /translation="VGKIFGPGNAYVTEAKRQVSSRPDGAAIDMPAGPSEVLVIADEG
                     ADPAFVAADLLSQAEHGPDSQVVLVTPSRKLAEAVAGEVASQLAALPRREIATKALSA
                     SRLILVKDLEACVEVSNRYAPEHLIIQTRNARDLVPLVQNAGSVFVGDWSPESGGDYA
                     TGTNHVLPTYGYAATYSSLGLADFTKRMTVQEMTPQGFEKLGPAIALLAHAEELDAHM
                     NAVLVRMDRIKQSKE"
BASE COUNT          542 a         1103 c         1176 g          582 t
ORIGIN      
        1 tgaactcctc gccgtacgtg cagcgggcgg tcgtgcaggc gcccgtgggc attgcgtagc
       61 tgtgcacgaa gtagaaccag tcgccggcct cgatgcccct gaagaggagt ctcgcatgag
      121 gcgtgtcctc gggatagacc ctgttccagc ccatgtgcgg cagcggcagg ccgtcggccc
      181 tgagggcgcg gacgtcggca tcgatgaggc cgagcaggtc cacgccgccc gtctcctcgc
      241 tgcggcgtcc gagaatctgt tcgccgaggc agatgccgag cacgggctgc gtcgcctcgc
      301 ggatgaggtc cgcaaggccg cgctcctcga tggcggccat cgcggctcgg gccgtgccca
      361 cgccgggcag gaagatgcgg tcggcggcgc ggatctcggc cgcctcgcgc gtaatgatgg
      421 gctcgacgcc gagcctcttc accgcaaagg cgagcgaaga gaggttggcg acgcccgtat
      481 caagaatgac ggcacgcatc agagcacccc cttggaggac ggcaggtcgc tcccctcaat
      541 tttcacagcg tcgcgcagcg tgcgcccgaa cgccttgaag agtccctcgg ccacgtggtg
      601 gtcgttcctg ccctgcgcct tgaggttgag cgtcacgccc atcgtctggg cgagcgagcg
      661 gaagaagtgc tcgaccatct cggtggacat gtcgcccacc ttgtggtgct tgaacttcgc
      721 cttgaaggtt aggtacggcc tgccagagat gtcgagcgcg cagcgcgcga gcgtctcgtc
      781 catcggcagc aggaagccga agcggcggat gccgcgcttg tcgccgagcg cctccttgag
      841 cgcctcgccg agcgcaaggc cgacgtcctc gatcgtgtgg tggtcgtcga tctcaaggtc
      901 gcccaccgca cgcacgtgca gtctgatgcc gccgtgcacg gcgatctgcg tgagcatgtg
      961 gtcgaagaaa cccacgcccg tgttgatttc attcttgccc gagcgatcga gccaggcttc
     1021 gacgacgatg cgggtctcct tcgtgttgcg ctcgacgcgg gcgtgacggg cggacttcgc
     1081 cgcattctcg atctcgtcgg cgatctcggc ccagctgcgg ccgtcgggcc cgacgcgaag
     1141 gcccctgagg cccatgttgt cggcgagctt catgtcggtc tcgcggtcac cgatcacgta
     1201 gcagttcgcg acgtcaagca cgccgtcctt gaggtacttc ttcacgaggc ccgttccggg
     1261 cttcctgcac tcacagccct cgccgggcat gtgcggacag atgagcacct cgtcgaagcg
     1321 ggcgccctgg ctctcgagga tctgcatcat cagttcgtgc ggccccgtga aatcggccct
     1381 cgggaagctc ttcgtgccga gcccgtcctg gttcgagacc atgacgagct tccagcccgc
     1441 ctcgcgcagg cgcttcaggg cgggcagaac gccctcgacg aacctcatct tctcgaagcg
     1501 gtcgacctga aagtcctcgg gctcctcgag aatggtgccg tcgcggtcca caaagagaat
     1561 gcgctcgttc atgcagcctc cttcagaatc tcttcaagtt cggtgagaag gcgctcgttc
     1621 tcgtccttcg tgcccaccgt gatgcggatg cagttctcga gcgtcggctg gcggctctgg
     1681 tcgcgcagga tgatgccgcg gtcgcgcaac gccgtgaaga cgcgggcggc gtcgacgaac
     1741 ctcacgagaa ggaagttcga ggcgctcgga tacacggcct cgacgccatc gaggtcctca
     1801 agcgcaacct caagctcaca cttgcgcatc acgcactggg cggcgcgcct gtgcatgagg
     1861 ctcacgctct cgggcgagag cgcctgcgcg gcgatgtcgg cgcagggcac gggaatcgga
     1921 tagggcgcga tcaccttcct cagcatgccg atgacggcgg gcgaggaaag caggaacccg
     1981 cagcgcaggc ccgccagcgc aaaggccttc gagagcgtgc ggatgatcac gacgtgcgga
     2041 tagtccttga gaagctcgac ggccgtgttc gcgggcgtaa attcgatgta ggcctcgtcg
     2101 atgacgagaa gagccctgcc cgcggtgagc tcggcgagct tgcgcagcat gcccacggga
     2161 atcagcgtgc ccgtcggatt gccgggcgag caggcgaaga cgagcttgac gtccgtcctc
     2221 tcctcgagga ccttcgcagc ctcggccacg tcgggcagcc agccgcggtc gggcgaggtg
     2281 acgaggttca cgacctcggc gccgttcgtc tcggcggaga cggcgtacat gccgtaggtg
     2341 ggcggaaact gcaggacggc gtcctcgccg gggcggcaga acgtgcgcac gagaagctcg
     2401 atgccttcgt ctccgcctcg cgtcacgagg atctgttcag gactcacgcc cgcataggcc
     2461 gcatagcgtt cgacgacggc ctcgggctgg ggctcgggat agcggttgaa gagcttcgtc
     2521 tcgggcgcca tcggctcggc ctcggccgac tcgttggcgt tgagccagac ttcgcccgtc
     2581 acgcccgcgg cctgcatgat gcggcgcgcg gactggtagg gcgtgagctt ctgcacgtcc
     2641 ggccgggcca gcaggcccgg atcggcggcg gttcgttcac aaaagacggc catgacggat
     2701 cactccttcg actgcttgat tcggtccatg cgcacgagaa cggcgttcat gtgcgcgtcg
     2761 agctcttcgg cgtgcgcaag aagcgcgatg gcggggccga gcttttcaaa gccctgcggc
     2821 gtcatttcct gaacggtcat gcgcttcgtg aagtcggcga gcccgaggct cgagtacgtg
     2881 gcggcatagc cgtaggtcgg aagaacgtgg ttcgtgcccg tggcgtagtc gccgcccgac
     2941 tcgggagacc agtcgcccac gaagacggag cccgcgttct gcacgagcgg caccagatcg
     3001 cgggcgtttc gcgtctggat gatgaggtgt tcgggcgcgt agcggttcga gacctccacg
     3061 cacgcctcga ggtccttgac gaggatgagg cggctcgcag acagggcctt cgtcgcgatc
     3121 tcgcggcgcg gcagggcggc gagctgggag gcgacctcgc ccgcaacggc ttcggcgagc
     3181 ttgcgcgagg gcgtcacgag caccacctgg ctgtcgggac cgtgttcggc ctgagagagc
     3241 aggtcggccg caacgaaggc cggatcggcg ccttcgtcgg cgatcacgag cacctcggag
     3301 gggcccgcgg gcatgtcgat ggcggcgccg tcgggacggc tgctcacctg acgcttggcc
     3361 tcggtcacgt aggcgttgcc cggaccgaag atcttgccca cgc
//
LOCUS       DBMDTI010000221         3394 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6337_length_3394_cov_2.798664, whole genome shotgun sequence.
ACCESSION   DBMDTI010000221 DBMDTI010000000
VERSION     DBMDTI010000221.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6337_length_3394_cov_2.798664"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="ACZPB6_08740"
     CDS             complement(<1..255)
                     /locus_tag="ACZPB6_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR1184591.1"
                     /translation="MALSIYSTLSRTQAPFAPIEPNHVRMYVCGVTVYDYCHIGHGRT
                     FVAFDVVRRWLEASGMKVTFVRNITDVDDKIIRRAAERGIT"
     gene            574..1113
                     /locus_tag="ACZPB6_08745"
     CDS             574..1113
                     /locus_tag="ACZPB6_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1184592.1"
                     /translation="MRRLITAALLALALGSAQAATQMEQLAKQMHPSEFPAQIEKLLN
                     SGKAAQALELADLGIRKNERSPQLRFMRAVALENLTRSEEAVRELKQLIIDYPEIPEP
                     YNNLAVIEAGLGNLEEAVAHLKQALRINPDFALARKNLGDVYLALAREAYEQAVVKLP
                     NNHVLERRLNTLKSLETAR"
     gene            1158..1739
                     /locus_tag="ACZPB6_08750"
     CDS             1158..1739
                     /locus_tag="ACZPB6_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1184593.1"
                     /translation="MKIRSRILLPLAPALLGLPGVVLAASPARDPRVEIVTDMGTIVV
                     QLAPSRAPRTVENFLRYVREGHYAGTVFHRVIQGFMIQGGGFTAEMQQKPVHDPIPLE
                     ARGGLPNDRYTIAMARTSYPHSATSQFFINVADNGFLNASQAADGQGYCVFGRVVKGQ
                     DVVDKIAEVRTGVKAGMRDVPVKPVTIVSARML"
     gene            1855..2346
                     /locus_tag="ACZPB6_08755"
     CDS             1855..2346
                     /locus_tag="ACZPB6_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1184594.1"
                     /translation="MIRFTTNKGVIDIELDHEHAPKSSANFEQYVRDGFYDGTIFHRV
                     IKGFMIQGGGFERGMKEKATRAPIENEAANGLKNNKYTIAMARTSAPHSASSQFFINV
                     VDNDFLNHTAPNPQGWGYAVFGKVTAGQNVVDEIARVPTSTHGFYGDVPVDDIVIEKA
                     EIL"
     gene            2431..3213
                     /locus_tag="ACZPB6_08760"
     CDS             2431..3213
                     /locus_tag="ACZPB6_08760"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR1184595.1"
                     /translation="MADKKVPALCGLKSVPELTNPVIISDVHLAANKPKTIMAFLRFM
                     KNVAPRFAELVILGDLFDYWLGDDATPEADPIIAELKLYSSTGRRVLIMQGSHDALLG
                     ADFAESCGAELIADQIVIPVNGNPILFAYGDQWCVRDQVRQARRAVLRDPIWQESLLA
                     QPAHERAAVIDEALRKCATENEAPKNPRDFDVIESAVAESARAAGVELVIHGHTHHPG
                     AHVNAVIERWVLPDWEFDLTETSCRSGYITFMQGGRPQIQMM"
BASE COUNT          672 a         1214 c          956 g          552 t
ORIGIN      
        1 cgtgatgccg cgctcggcag cgcggcggat gatcttgtcg tcgacgtccg tgatgttgcg
       61 cacgaaggtc accttcatgc cggaggcctc gagccagcgg cgcacgacgt cgaaggccac
      121 gaaggtgcgg ccgtggccga tgtggcagta gtcgtagacc gtcacgccgc agacgtacat
      181 gcggacgtgg ttgggttcga tcggggcaaa gggggcctgc gtgcggctga gcgtcgaata
      241 gatcgaaagt gccatgggtg aggagtcctt ctactcttat atataggcgt ctgcgcgcat
      301 gaaggcgcgt gcgcaggggc gggatgcgcc ccgcgcttgc atgcgtgcgt cctgcggagc
      361 cgtgagacgg gaatcatccg ccgtgaggcc gcagtgggct aaaatgtggc gcagactgaa
      421 acatcgctgc aggaagtata gccgtcccgc cccctcgctg ccgtcgcgaa tcgagcgcaa
      481 gccgcttttt tgcgtgcgcc ctagcctcaa gcggggatgc ccggtcatcc tttccgccga
      541 cgcccatcat ccaagacaga agagaagata cacatgcgac gcctcattac cgccgcgctc
      601 ctcgcgctcg cactcggctc cgcccaggcc gccacccaga tggagcagct cgccaagcag
      661 atgcacccga gcgaattccc ggcccagatc gaaaagcttc tcaacagcgg caaggccgcc
      721 caagcgctcg agctcgccga cctcggcatc cgcaagaacg aacgcagccc gcagctgcgc
      781 ttcatgaggg ccgtggcgct cgaaaatctc accaggtccg aagaggccgt gcgcgagctc
      841 aagcagctca tcatcgacta tcccgaaatc cccgagccct acaacaacct cgccgtgatc
      901 gaggcgggtc tcggcaacct cgaggaagcc gtcgcgcacc tcaagcaagc cctgcgcatc
      961 aaccccgact tcgcgcttgc gcgcaagaac ctcggcgacg tgtatctcgc gctcgcccgc
     1021 gaggcctatg aacaggccgt cgtgaagctt ccgaacaacc acgtcctcga gcgccgcctc
     1081 aacacgctca agagcctcga gaccgcccgc tgagccgggc tgcgcgcaac ccaaaaccaa
     1141 gacagacaaa aaatattatg aaaatccgct cccgtatcct tcttccgctc gcgcccgccc
     1201 ttctgggcct tcccggcgtc gtcctcgccg cctcgcccgc ccgcgacccg cgcgtcgaga
     1261 tcgtcaccga catgggcacg atcgtcgtac agctcgcccc gtcgcgcgcg ccccgcacgg
     1321 tcgagaactt cctgcgctac gtccgcgagg gccactacgc gggcacggtc tttcaccgcg
     1381 tgatccaggg cttcatgatc cagggcggcg gcttcaccgc cgagatgcag cagaagcccg
     1441 tgcatgatcc gattccgctc gaggcacgcg gcggcctgcc caacgacagg tacacgatcg
     1501 ccatggcccg cacctcctac ccgcactctg cgacctcgca gttcttcatc aacgttgcgg
     1561 acaacggctt cctcaacgcc tcccaggccg ccgacggcca gggctactgc gtcttcggcc
     1621 gcgtcgtcaa gggccaggac gtggtcgaca aaatcgccga ggtccgcacg ggcgtcaagg
     1681 ccggcatgcg cgacgtgccc gtcaagcccg tgacgatcgt ctccgcgcgc atgctctgag
     1741 acacggagaa cgagcctctc gcacagccct gctttgtgcg acaatacgcc attgcccgcc
     1801 gccccgcggg gcggcggatg tttgaatgaa ttacgttttt agactgacaa gacaatgatt
     1861 cgctttacga ccaacaaggg cgtgatcgac atcgagctcg atcacgaaca cgctcccaag
     1921 agttccgcca acttcgaaca gtacgtccgc gacggcttct acgacggcac gatcttccat
     1981 cgcgtcatca agggcttcat gatccagggc ggcggcttcg aacgaggcat gaaggagaag
     2041 gccacccgcg ccccgatcga aaacgaagcc gccaacggtc tcaagaacaa caagtacacc
     2101 atcgccatgg cccgtacgtc cgccccgcac tccgcgtcgt cccagttctt catcaacgtc
     2161 gtcgacaacg acttcctgaa ccacacggct ccgaacccgc agggttgggg ctacgccgtc
     2221 ttcggcaagg tcacggccgg ccagaacgtc gtcgatgaaa tcgcccgcgt tcccacctcc
     2281 acgcacggct tctacggtga cgtgccggtt gacgacatcg tgatcgaaaa ggccgaaatc
     2341 ctctgataga gcccgctcaa cacgagcctc tggggcgccg agatgacacg tccggcgccc
     2401 ttccgttcat ttagactgga caaatccgcc atggcagaca aaaaggttcc ggccctctgc
     2461 ggcctcaaga gcgtgcccga gctcaccaat cccgtcatca tctccgacgt gcatcttgcg
     2521 gccaacaagc ccaagaccat catggcgttc ctgcgcttca tgaagaacgt cgcgccgcgc
     2581 tttgccgaac tcgtcattct gggcgacctc ttcgactact ggctcggcga cgacgccacg
     2641 cccgaagccg acccgatcat cgcagaactc aagctctact cctcgaccgg ccgccgcgtg
     2701 ctcatcatgc agggctccca cgacgccctt ctgggcgccg acttcgccga gtcctgcggt
     2761 gccgagctca tcgccgacca gatcgtcatt cccgtcaacg gcaatccgat cctcttcgca
     2821 tacggcgatc agtggtgcgt ccgcgaccag gtccgccagg cccgtcgcgc cgtactgcgc
     2881 gacccgatct ggcaggaaag cctcctcgcc cagcccgccc acgagcgcgc cgccgtcata
     2941 gacgaggccc tgaggaagtg cgccaccgaa aacgaggctc cgaagaaccc gcgcgacttc
     3001 gacgtcattg aaagcgccgt cgccgaatcg gcccgcgccg caggcgtcga gctcgtcatc
     3061 cacggccaca cccaccaccc cggcgcccac gtcaacgccg tcatcgagcg ctgggtgctc
     3121 cccgactggg agttcgacct caccgaaacg agctgcagga gcggctacat caccttcatg
     3181 caaggcggcc gcccgcagat tcagatgatg tgacgtctgc gcccgaccgt tcaggaaggg
     3241 atcgacacct catgcccgga cgccgaaagg cctccgggct ttttcgtctt cacacgcgtc
     3301 gttcgtgaaa aaatccgaac gtccgcgcat tttgccgtaa aatgtcgcat gttctcaaac
     3361 aagcccgctc gtccgacaac cccgcgggac gagc
//
LOCUS       DBMDTI010000222         3376 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6377_length_3376_cov_1.873588, whole genome shotgun sequence.
ACCESSION   DBMDTI010000222 DBMDTI010000000
VERSION     DBMDTI010000222.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6377_length_3376_cov_1.873588"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="ACZPB6_08765"
     CDS             complement(<1..871)
                     /locus_tag="ACZPB6_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HIR1184596.1"
                     /translation="MQPTETRFLTRASAGPEQWQSAVSGYASDPIRRLVPLSDTNPDD
                     CLVAHPADGRLRTILAPEDPVRMLLRHRAAVEQLRMAVGSRKLFEAHLMPAMAGWAAM
                     VQGLPRDERGLWSGPDGLFEAGLMFARGAINAVDARVIGPELAPGIRDDWTLRLRIAS
                     ALAGLMSDSRKLAALKLEAGSEDPATGTFTVTSRFNPSEETALSFCVHEIGRVMRLER
                     HTARESAGRLPTLNADVLRLVVPRETLMWLGVIERPDGETVLTALKAAVTYAAGQTEA
                     ERLIAEAVARGREW"
     gene            complement(1160..2959)
                     /locus_tag="ACZPB6_08770"
     CDS             complement(1160..2959)
                     /locus_tag="ACZPB6_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012321.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR1184597.1"
                     /translation="MRTVIVEDVETAEAKKQRLATTAVDAALEILRTEASGLVIEAAA
                     PLVFQGLCRRDNIRVEFTQTPMTNGRVIWLGPIDLGHKLASIYVYGHGCHERHHVMYT
                     NFAAMRGIGDPGVRDLANVFEDIRVDQLGAADYEGYLLWRLVLFKAHEATGTAGWSNP
                     NDLEPAHLFAMMLLTTLEFEVLGIDMFEEISANLRGECDRLFGPRPMARVLKIVRAAF
                     PLDSTEAAVELAHKVMRCIRAEKNKALKAVEEAAGEGSTDNFEFRQGEGQSLLFDDDG
                     AVTPEGDPRLMHPELEAINRNARCLSVISDQRQWPDREPEEESFRKLIGNGESGNGDP
                     NFASPERHFANDREYPTLQPEKAAMNRRLFYRALANSASLRRLLQSALIHPQSNPDRL
                     DERGEELDGDALALASTGETAVFRRELVRPGREAVVEIILDASGSMEGEAITLAKVAC
                     IRLLEALRAVTGVKASLTIFPGPNRRSVTCAADFNTPVSRALKHVDFLPGFGATPILQ
                     ALFHSAIVLDQRPEPAKIVFVITDGWFPKGPVDNLVKALEDRGIAVAMVGIGRHSTPK
                     GSIIAKAETIADLPRAVAKVLSGLAGKLRGIAA"
     gene            complement(3145..>3376)
                     /locus_tag="ACZPB6_08775"
     CDS             complement(3145..>3376)
                     /locus_tag="ACZPB6_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184598.1"
                     /translation="RNMMLDGARMPAASDDDPLRRIIRTSLTEALDSTAREAAETLAV
                     TAIGNLIHEMRVSRARMVLKNATGSALKAASV"
BASE COUNT          584 a         1012 c         1156 g          624 t
ORIGIN      
        1 cccattcgcg accgcgggct acggcttcag caatcaggcg ttcggcctcc gtctggccgg
       61 ccgcataggt gacggcggcc ttgagagccg tgagaacggt ctcgccgtcg gggcgctcga
      121 tgacgccgag ccacatcagg gtttcacgag ggacgacgag acgaaggacg tcggcgttga
      181 gcgttgggag tcttcccgca gattcacgcg ccgtgtggcg ctcgagcctc attacgcgcc
      241 cgatctcgtg cacgcaaaac gacagtgccg tttcttccga aggattgaag cggctcgtga
      301 cggtgaaggt gcccgtggca ggatcctcgc ttcccgcctc gagcttcagt gcggcgagct
      361 tgcggctgtc gctcatgaga ccggcgagtg cggaggcgat tcgaaggcga agcgtccagt
      421 cgtcacgaat gcccggcgcg agttcgggac cgatcacgcg tgcgtcgacc gcattgatcg
      481 cgcctcttgc aaacatgagg ccggcttcga agaggccgtc cggccccgac cagagccccc
      541 tttcgtctcg aggaagtccc tggaccatcg cggcccagcc cgccatggcg ggcatgaggt
      601 gcgcttcgaa aagcttgcgt gatcccacgg ccatcctcag ttgctcgacg gcggccctgt
      661 gacgaaggag catgcggacg ggatcctcag gcgcgaggat cgtgcgcaga cgcccgtcgg
      721 cgggatgcgc gacaagacag tcgtcaggat tcgtgtccga gagcggcaca agccttcgga
      781 tcgggtcgga cgcgtacccc gagacggcgc tctgccactg ctcggggcct gcggaggcgc
      841 gcgtgaggaa tcgtgtttct gtgggctgca tgggagattg gagggaggaa ggacgcaagg
      901 ctggatgcgg ggtgaaatgg gtgaaacagg ttcgcagtgc gccggcctct tcgtgtgagg
      961 atgatgcgga gaatgatggg cgggagacgg aacgggcgcg aagaggacgc cgcactgcgg
     1021 cctcaggcag ggagcctgaa gcgaagggag atgattgaag aatcgacggc ggccggacga
     1081 cggaggagaa gtcctccgtc gatcccggtc gtgtcgaaaa gtcgaagacg cgtgccttca
     1141 gatgcccctt gggtttcgcc tatgcggcga tcccgcggag cttgcccgca aggcccgaaa
     1201 ggacctttgc aacggcccgc ggaaggtcgg cgatcgtttc ggccttggcg atgatgctcc
     1261 cctttggggt gctgtgcctg ccgatgccga ccattgcgac ggcgatgccg cggtcttcca
     1321 gtgccttgac gaggttgtcg accggcccct tggggaacca gccgtcggtg atgacgaaga
     1381 cgatcttcgc gggctccggt ctttgatcga gaacgatcgc agagtgaaag agcgcctgaa
     1441 ggatcggcgt cgcaccgaac cccggaagga agtcgacgtg cttgagcgca cggctcaccg
     1501 gcgtgttgaa gtccgccgcg caggtgacgc tgcgacggtt cgggccgggg aagatcgtca
     1561 gggatgcctt cacgccggtc accgcacgga gcgcctcaag gaggcggatg caggcgacct
     1621 tggcaagcgt gatcgcctcg ccttccatgg aacccgaagc gtcgagaatg atctcgacga
     1681 ccgcttcgcg acccggacgc acgagctcgc ggcggaagac cgccgtttca cccgtggagg
     1741 cgagcgcaag cgcatctccg tcgagctcct ctccacgttc gtcgagcctg tcggggttcg
     1801 actgcgggtg gatgagcgcg ctctgaagga gccttctgag cgatgcgctg ttggcgagcg
     1861 ccctgtagaa gagtcggcgg ttcatcgccg ccttctcagg ctgcagcgtc ggatactcgc
     1921 ggtcattggc gaagtggcgc tcgggcgaag cgaagttcgg atcgccgttg ccggattcac
     1981 cgttgccgat gagcttgcgg aagctttcct cctcgggctc gcgatcgggc cactgccgtt
     2041 ggtctgaaat gaccgagagg catcgggcgt ttcggttgat cgcctcaagc tcggggtgca
     2101 tcagacgcgg atcaccctcg ggggtcaccg cgccgtcgtc gtcgaagaga agcgactggc
     2161 cttcgccctg acggaactcg aagttgtccg tagagccttc gcctgccgct tcctcgacag
     2221 ccttgagcgc cttgttcttc tcggcgcgga tgcaccgcat gaccttgtgc gcaagttcca
     2281 ccgccgcctc cgtggagtcg agcggaaagg ctgcgcgcac gatcttcagg acgcgggcca
     2341 tgggccttgg tccgaagagc cggtcgcact ccccgcgaag gttcgcggag atctcctcga
     2401 acatgtcgat cccgagcacc tcgaattcga gggtcgtgag cagcatcatt gcgaagaggt
     2461 gtgcgggctc aaggtcgttc ggattgctcc agccggccgt ccccgtcgcc tcgtgcgcct
     2521 tgaagagcac gagtcgccag agcaggtagc cttcgtagtc cgcagcaccg agctggtcca
     2581 cgcggatgtc ctcgaagacg ttcgcgaggt cgcgaacgcc gggatcaccg atccccctca
     2641 tcgccgcgaa gttggtgtac atcacgtgat gacgttcgtg gcatccgtgt ccgtagacgt
     2701 agatggacgc gagcttgtgg ccgaggtcga tgggaccgag ccagatcacg cgcccgttcg
     2761 tcatcggggt ctgcgtaaac tcgacgcgga tgttgtcgcg gcggcagagt ccctggaaga
     2821 ccaggggcgc tgcagcctcg atgacgaggc ccgaggcctc cgtgcggaga atctcgagcg
     2881 ctgcatccac ggcggtggtg gcgagcctct gctttttcgc ttcggcggtt tcgacgtctt
     2941 ccacgattac ggtgcgcatc gttcgcctcc ttcgaaaagg atgttgaacg gatgttgcac
     3001 tgcaaacgca gcgggggcct gattcccgcg catgtcaaga ctgcgtttca tgacggtcgt
     3061 ctctcaaatg aatgaggaga cgtccgtccc cgagagggat gaagcatccc ctcggggttg
     3121 atgaaaaggt ccttccgacg attgtcagac ggacgctgcc ttgagcgcag agcccgtggc
     3181 gttcttgagg accattctgg cgcgtgacac cctcatctcg tgaatgaggt tgccgatcgc
     3241 cgtgaccgcc agcgtctccg ccgcttcgcg agcggtggag tcgagggctt cggtcagact
     3301 ggtcctgatg atcctgcgaa gaggatcatc gtcggaagct gcgggcatcc ttgcgccatc
     3361 gagcatcatg ttccga
//
LOCUS       DBMDTI010000223         3355 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6409_length_3355_cov_2.514136, whole genome shotgun sequence.
ACCESSION   DBMDTI010000223 DBMDTI010000000
VERSION     DBMDTI010000223.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6409_length_3355_cov_2.514136"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="ACZPB6_08780"
     CDS             complement(<1..1162)
                     /locus_tag="ACZPB6_08780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184599.1"
                     /translation="MSHTDMSRRGLLKTSFLGAAGAAAAGATGFGLSSQAQAKQAANA
                     QTYDAVVLGAGPAGLIAAITAHDAGAKVVVLEKRDRPDGNAIFALGSICGWGTRHQAE
                     QGIKDTADDFYAMMMDVSKQMGDKALNRCYTDNISAGIDWLEKDIGVKFGKIRPMPYP
                     RLGRTCRVMGEGLTGGAQLVQKLLAACAKRGIEVKYQHKAVELIHDDLYAVKGVKAAT
                     PEGFRTFMARGGVCIATGGFSANPEMVDRYIGGWATRMVLRGSKSTTGENISLCMPLY
                     TKFVNMDQFHSGPIIGATHVNPADVLNSGYGVQFNTSGVRFMDENNTYVIKARTTGQM
                     TLDNMAWVIVDSTCPVLDKVIPKFDRLNSPYGKADTIEELCRQVKLPVEKVVK"
     gene            complement(1370..>3355)
                     /locus_tag="ACZPB6_08785"
     CDS             complement(1370..>3355)
                     /locus_tag="ACZPB6_08785"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR1184600.1"
                     /translation="AALVCDHLVQANVGAIPDLKVAEKGNVETYEFLRDVSRRYGFDF
                     WPAGSGICHQVFMENYNFPGCMMLVTDSHTPTACGMGMLAIGVGGADLVDALMGMEWE
                     LKMPKIIGVKLTGQLQGWASAKDVILKLTGMLSTKGATNCVVEFFGEGAATLSASGKA
                     TIGNMGAEMGATTSVFAYDDSMARYLAATGRQAVADLAAKVDLAADKEVYADPAKYYD
                     RVVEINLSELEPHINGPFSPDAAMPISKVAETIKAKGYPQQLQVALIGSCTNSSYEDI
                     TRAASIARQALENGVEIKTPLMVVPGSTRIYDTLKRDGVLEVFEKLHATVLATACGPC
                     IGQWKRTIGNLKGDKTPNSIIESFNRNFAGRNDGNPLTNAFLASPEMVMAMAINGDLT
                     FNPLEGTVRTAKGTNMKLAAPVGQELPAKGFVSDIKGCVIPDFEKIEIKVSPTAERIR
                     LLEPFPAWDGQDFTALPLLIKAKGKCTTDHISPGGKWLPYRGNIDRISDNLLERAVNA
                     FNGVSGKVWNTDTNAYDTPAKVARHYRNNNIASVIVGDDNYGEGSSREHAAMEPRYLN
                     VRVVLAKSLARIHESNLKKQGILALTFVNPADYDKIQEKDRISVLNLNTLAPNSRVVI
                     ELTHENGTKERFEAKHSYNEKQLSWFRAGSALNALKA"
BASE COUNT          527 a          900 c         1226 g          702 t
ORIGIN      
        1 tcttgacgac cttttcaacc gggagcttga cctgcctgca gagctcttcg atcgtgtcgg
       61 ccttgccgta cgggctgttg aggcggtcga acttcggaat gaccttgtcg aggaccgggc
      121 aggtgctgtc gacgatgacc caggccatgt tgtcgagcgt catctgaccg gtggtgcggg
      181 ccttgatcac gtacgtgttg ttttcgtcca tgaagcgaac gccggaggtg ttgaactgga
      241 cgccgtagcc ggagttgagg acgtcggccg ggttcacgtg cgttgcgccg atgatcgggc
      301 cggagtggaa ctggtccatg ttcacgaact tcgtgtagag cggcatgcac agcgaaatgt
      361 tttcacccgt cgtggacttg gagccgcgca acaccatgcg ggtggcccag ccgccgatgt
      421 agcggtcgac catttcgggg ttggccgaga agccgcctgt ggcgatgcag acgccgcctc
      481 tggccatgaa ggtcctgaag ccttccggtg tggcggcctt gacgcccttc acggcataga
      541 ggtcgtcgtg aatgagctca acggccttgt gctggtactt cacttcgatg ccgcgcttgg
      601 cgcaggcggc gagaagcttc tgcacgagct gggcgccgcc ggtgaggcct tcgcccatga
      661 cgcggcaggt gcggcccaga cgcggatacg gcatcgggcg gatcttgccg aacttgacgc
      721 cgatgtcctt ttcgagccag tcgatgccgg cggagatgtt atccgtgtag cagcggttga
      781 gggccttgtc gcccatctgc ttggagacgt ccatcatcat ggcgtagaag tcgtcggccg
      841 tgtccttgat gccctgttcg gcctggtggc gcgtgcccca gccgcagatg gagccgaggg
      901 cgaagatggc gttgccgtcg ggacggtcgc gcttttcgag cacgacgacc ttggcgccgg
      961 cgtcgtgggc cgtgatggcg gcgatcaggc cggcaggacc tgcgccgaga acgacggcgt
     1021 cataggtctg ggcgttggcg gcctgttttg cctgggcctg ggaggagagg ccgaagccgg
     1081 tggcgccggc agcggcggca ccggcggcac cgaggaagga ggtcttgaga agaccgcggc
     1141 ggctcatatc ggtatgagac attgttgatc tcctgggaat ccgggtcggg gattccgtca
     1201 tttaggggga agaatctgta gggtgggcgg gactccatcc ggtttctgtg gcttttgtga
     1261 gccccgccct gttgtcgtcg aggagctgta tgcattgtcc tcttgaggca ccactacgta
     1321 gtgccgcatg aggtcaacga aacgtcactt tctgaagcgt ctcttaaggt caggccttga
     1381 gagcgttcag ggcggagccg gcacggaacc agctcaactg cttctcgttg taggagtgct
     1441 tcgcctcgaa gcgttccttc gtgccgtttt catgcgtgag ttcgatgacg acgcggctgt
     1501 tgggcgcaag ggtgttcagg ttgagaaccg agatgcggtc cttttcctga atcttgtcgt
     1561 agtcggccgg attcacgaag gtgagtgcga ggatgccctg cttcttgagg ttggactcgt
     1621 ggatgcgggc gaggctcttg gcgaggacga cgcggacgtt gagatagcgc ggttccatgg
     1681 cggcatgttc gcgcgaggag ccttcgccgt agttgtcgtc gccgacgatc acgctggcga
     1741 tgttgttgtt gcgatagtgg cgcgcaacct tggcaggcgt gtcataggcg ttcgtgtccg
     1801 tattccagac cttgccggag actccgttga aggcgttgac ggcgcgttcg agcaggttgt
     1861 ccgaaatgcg gtcgatgttg ccgcggtacg gaagccactt gccgcccggg gagatgtggt
     1921 cggtcgtgca cttgcccttg gccttgatga ggagcggaag ggcggtgaag tcctggccgt
     1981 cccaggcggg gaacggctcg agcaggcgga tgcgttcggc cgtcgggctc accttgatct
     2041 cgatcttctc gaagtcggga atgacgcagc ccttgatgtc ggagacgaag cccttggcgg
     2101 ggagttcctg accgaccggg gcggcgagct tcatgtttgt acccttggcg gtgcggaccg
     2161 tgccctcgag cgggttgaac gtcaggtcgc cgttgatggc catggccatg accatttcag
     2221 gcgaggcgag gaaggcgttg gtgagcgggt tgccgtcgtt gcggccggcg aagttgcggt
     2281 tgaaggactc gatgatggag ttgggcgtct tgtcgccctt gaggttgccg atcgtgcgct
     2341 tccactggcc gatgcaggga ccgcaggcgg tggcgaggac ggtggcgtgg agtttttcga
     2401 agacctcgag gacgccgtcg cgcttgagcg tgtcgtagat gcgggtcgag ccgggaacga
     2461 ccatcagcgg ggtcttgatc tcgacgccgt tttcgagtgc ctgacgggcg atggaggcgg
     2521 cgcgggtaat gtcctcgtag gaggagttcg tgcaggagcc gatcagggcg acctgaagct
     2581 gctgcggata gcccttggcc ttgatcgttt cggcgacctt cgagatcggc atggcggcgt
     2641 cgggcgagaa cggaccgttg atgtgcggtt cgagttcgga gaggttgatc tcgacgacgc
     2701 ggtcgtagta cttggccggg tcggcataga cttccttgtc ggcggcgagg tcgaccttgg
     2761 cggcgaggtc ggcaacggcc tgacggcccg tggcggcgag gtagcgggcc atggagtcgt
     2821 cgtaggcgaa gacggaggtc gtggcgccca tttcagcacc catgttgccg atcgtggcct
     2881 tgccggaggc ggagagcgtg gcggcgcctt cgccgaagaa ctcgacgacg cagttcgtgg
     2941 cgcccttggt gctgagcatg ccggtgagct tgaggatcac gtccttggcg gaggcccagc
     3001 cctgaagctg gccggtgagc ttgacgccga tgatcttcgg catcttgagc tcccattcca
     3061 tgcccatgag ggcgtcgacg aggtcggcac cgcccacgcc gatggcgagc atgcccatgc
     3121 cgcaggccgt cggggtgtgg gagtcggtca cgagcatcat gcaacccggg aagttgtagt
     3181 tttccatgaa gacctgatgg cagatgccgg agccggcggg ccagaagtca aagccgtagc
     3241 ggcgggagac gtcgcgcagg aactcgtacg tttcgacgtt gcccttttcg gcgaccttga
     3301 ggtcgggaat ggcgccgacg ttggcctgaa cgaggtggtc gcagacgagc gctgc
//
LOCUS       DBMDTI010000224         3318 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6482_length_3318_cov_2.060305, whole genome shotgun sequence.
ACCESSION   DBMDTI010000224 DBMDTI010000000
VERSION     DBMDTI010000224.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6482_length_3318_cov_2.060305"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="ACZPB6_08790"
     CDS             complement(<1..313)
                     /locus_tag="ACZPB6_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR1184601.1"
                     /translation="MAQTSRKHALIIVDLQKDFLPGGALAVPGGDKIVPVIHEVLEKN
                     SWDCIVMTQDWHPAGHVSFARNHEGRHVLDVIELPYGMQMLWPVHCVEGTDGAAIEGV
                     DT"
     gene            580..1824
                     /locus_tag="ACZPB6_08795"
     CDS             580..1824
                     /locus_tag="ACZPB6_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HIR1184602.1"
                     /translation="MTDIAGTVPVIAVDKVQAVALAVCTYYFGVWLKGKVGVLSRYSI
                     PSPVVGGMSFAIVVSFLEYFNIVKVQIDSTLQTVLMLGFFTTIGLMASLRVVKDGGRL
                     LLGFLLAVTVLVFLQNGMGMAIAEAMGFDKHYGILAGSVSMMGGLGTAAAFGPYCEET
                     YGITGGTAVAVTAATFGMVAALLIGGPFGEWCIRRYQVKTPDEVQQPEPELHLPDDMD
                     TDITEQHASAKPNFTNEIMRAVSVTALAMGLGTIVSNYLGHYITLPAYIGAMIVATVI
                     RNVGDFSGMYKVEGKGLNAVADITLVLFVTMAINNLKLHELVHLALPLVVILACQTIL
                     MLIYAWTVLFTAFGRSYDAVMLSVGGIGFSMGATANGLANMQAISEKYGSSPRAWLIV
                     SVVGAFLIDLINAVVITWFGTL"
     gene            1850..2035
                     /locus_tag="ACZPB6_08800"
     CDS             1850..2035
                     /locus_tag="ACZPB6_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184603.1"
                     /translation="MSDQALVSQPDTAQEVLRGLRAAVPVMTGFVPVGLILGATGAHA
                     GLSPFTSGFIETPQGNI"
     gene            complement(2103..>3318)
                     /locus_tag="ACZPB6_08805"
     CDS             complement(2103..>3318)
                     /locus_tag="ACZPB6_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184604.1"
                     /translation="FYLEREEEALPCFERAEALQKGDQDSLEFMRLCRFQMAFPRFSK
                     PLRERINDFWDGMAQKTPGLLKRLADPDECQDAQLEITEDLQKVVPGALAYVGAIAGP
                     VDLNLSADGDWLRTYLLRALIRRMPEELRSDWRLTLGRRRGSIPLRFDINGRELHGGN
                     VYLWEEKPEDGGADRLIAYHEAFETLEEPEYAEAFSCVQALLDNAVGEAVRMKYYYDL
                     RLTRKPESGVGFSVQEFQSYLRETDPMKLVTTMDDYLDESNEFDWDGSEEKDCDYLLD
                     AVHAELSCPALVLAYFKNDPDLMRMLAQSGATAGTLYMNTRDRSPEGRREVRDSLRTE
                     LQANVPEAYESVGIVDGRRYAYDVFLAWDLPAVLEAAREFAEGRDDVLELGFHSLWRE
                     AGGLSIRKPEKA"
BASE COUNT          636 a         1064 c          930 g          688 t
ORIGIN      
        1 tcgtgtcgac gccctcgatg gcagcaccgt cggtgccttc aacgcagtga acgggccaga
       61 gcatctgcat gccgtatgga agttcaatga cgtcgagcac gtgcctgcct tcatggttcc
      121 gggcaaagga gacgtgaccc gcaggatgcc agtcctgggt catgacgatg cagtcccagg
      181 aattcttttc gagcacttca tggatgacgg gcacgatctt gtcgccgccg ggtaccgcca
      241 gcgcgccccc gggcagaaaa tccttctgca ggtcgacgat gatcagggcg tgctttcgtg
      301 aggtttgtgc catttcttag actccggcag cggttgaacg tttgtgattc cgcggacgac
      361 ttccacggca gttgttcgat tttaggggac ctggtgcgga ccttgagccc gagtcaggat
      421 aaaccctgca gggtttggcc tttgattgag aaatgtcgac tccccttggg cgaatgcctt
      481 agggaaggca ccttgagccg ctttcccaca ctgctagact tttcatcagc ctgcctgaaa
      541 atcaggcgtt cacaacaacc aacaaaaaga gaaggaaaca tgacagatat agcaggaacg
      601 gtgccggtca tcgcagtgga caaggtgcag gccgtcgcgc tcgcagtatg cacctattat
      661 tttggcgtct ggctcaaggg aaaggtcggc gtgctttcac gctattccat cccgagtccc
      721 gtggtgggcg gcatgtcctt tgccatcgtc gtctccttcc tcgagtactt caacattgtc
      781 aaggttcaga tcgactcgac gctgcagacc gtgctcatgc tcggcttctt cacgaccatc
      841 ggcctgatgg ccagccttcg cgtcgtcaag gacggcggca ggctccttct cggcttcctc
      901 ctcgcggtca cggtacttgt gtttctccag aacggcatgg gcatggccat agcggaagcc
      961 atgggctttg acaagcacta cggcattctc gcgggctccg tctccatgat gggcggcctc
     1021 ggcaccgccg ctgccttcgg tccctattgt gaggaaacct acggcatcac gggcggcacc
     1081 gccgttgccg tcaccgccgc caccttcggc atggtcgccg cccttctcat cggcggcccg
     1141 ttcggcgaat ggtgcatccg ccgctaccag gtgaagactc ccgacgaagt ccagcagcct
     1201 gaacccgagc tgcacctccc cgacgacatg gacaccgaca ttacggaaca gcacgcatcc
     1261 gcgaagccaa acttcacgaa tgagatcatg cgggcggtct ccgtcacggc gctcgccatg
     1321 ggcctcggca ctatcgtctc gaactacctc ggccactaca tcacgcttcc cgcctacatc
     1381 ggtgccatga tcgtcgccac cgtcatccgc aacgtcggag acttctcggg gatgtacaag
     1441 gtcgaaggca agggcctcaa cgcagtggcc gacatcacgc tcgtcctctt cgtcacgatg
     1501 gccatcaaca acctgaagct ccacgagctc gtgcacctcg cgctcccgct cgtagtgatc
     1561 ctcgcctgcc agacgatcct gatgctcatc tacgcctgga cagtgctctt cacggccttc
     1621 ggccgctcct acgacgccgt catgctctcc gtgggcggca tcggcttctc catgggtgct
     1681 acggccaacg gtcttgccaa catgcaggcc atcagcgaaa agtacggctc gtccccgcgc
     1741 gcctggctca tcgtctcggt cgtgggcgca ttcctcatcg acctgatcaa cgccgtcgtc
     1801 atcacgtggt tcggcacgtt gtaacgacag gctctcttct gatctgaaga tgtcagacca
     1861 agctctcgtt tcacaacccg acacggcgca ggaggtcctc aggggccttc gtgccgccgt
     1921 gcccgtcatg acgggcttcg ttccggtcgg cctcattctc ggcgctacgg gggcacatgc
     1981 cgggctctcg cccttcacct cgggcttcat agaaacgccg caaggaaaca tctgacttca
     2041 ggaagccgcg gccaaggcat ttcgaaaatc gtgtcaacct cgtgcacggc ctccgtagcc
     2101 gatcaggctt tttcgggctt cctgattgaa agaccgcccg cttcgcgcca aagggagtgg
     2161 aacccgagct cgagcacgtc atcgcgacct tctgcaaatt cccgcgcagc ctcgagcact
     2221 gcggggagat cccaggcaag gaacacgtca taggcatacc gcctgccgtc gacgatgccg
     2281 acactctcat aagcttcagg aacatttgcc tgcagctcgg ttctgagcga atctcgaacc
     2341 tcccttcgac cttcgggtga ccgatcgcgg gtgttcatgt aaagggtgcc cgccgttgcg
     2401 cccgactgcg cgagcatgcg catgagatcg gggtcgtttt tgaagtaggc gaggacaagt
     2461 gccggacagg acagctcagc atgcacggca tcgagcaggt aatcgcagtc cttttcttcg
     2521 gatccgtccc agtcgaattc attcgactcg tcgagatagt catccatcgt cgtgacgagc
     2581 ttcatcggat cggtttcgcg caggtagctc tggaattcct gcaccgagaa gccgacgccc
     2641 gattcaggct tgcgcgtgag gcgaagatcg tagtaatact tcatccgcac cgcttcgccc
     2701 acggcattgt caaggagcgc ctgcacgcac gaaaaggctt cggcatactc gggctcctcg
     2761 agagtttcga aggcttcatg gtaggcgatc aaccggtctg cacccccgtc ctccggcttt
     2821 tcctcccaga gatagacgtt accgccatgg agctccctgc cgttgatgtc gaaccgcagc
     2881 gggatcgacc ctctgcggcg tccgagcgtc aagcgccagt cggacctcag ctcttcgggc
     2941 atccgtcgga tgagtgcccg cagaagatag gtcctcagcc agtcgccgtc ggccgagagg
     3001 ttgagatcca ccgggccggc aatggctccg acgtacgcca gagctcccgg gacgaccttc
     3061 tgaaggtcct cggtgatctc gagctgcgca tcctggcatt cgtcaggatc ggccagacgc
     3121 ttcagcagcc caggcgtctt ctgcgccatg ccgtcccaga aatcgttgat acgctcccgc
     3181 agcggtttcg agaaacgagg aaaggccatc tgaaaacggc acagtcgcat gaattcaagt
     3241 gaatcctgat cgcctttctg cagcgcttct gcacgctcga aacagggcag cgcctcctcc
     3301 tcgcgttcga ggtaaaag
//
LOCUS       DBMDTI010000225         3309 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6506_length_3309_cov_2.946072, whole genome shotgun sequence.
ACCESSION   DBMDTI010000225 DBMDTI010000000
VERSION     DBMDTI010000225.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6506_length_3309_cov_2.946072"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            16..123
                     /locus_tag="ACZPB6_08810"
     CDS             16..123
                     /locus_tag="ACZPB6_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013228.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HIR1184605.1"
                     /translation="MTEVMSEPYPARSCFAVAALPKAARVEIEAVVTMP"
     gene            148..2241
                     /gene="recG"
                     /locus_tag="ACZPB6_08815"
     CDS             148..2241
                     /gene="recG"
                     /locus_tag="ACZPB6_08815"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR1184606.1"
                     /translation="MQNMAAAPKQQERASGVIRQEKLAPLDVRLEKLGLVRDWDFALH
                     LPLRYEDETSVTPIGSLAVNTHAQIEGRVADQRFVRTGRGQQLQAVVEDSTGSITIRF
                     LHYFPSIQKQLAVGSTVRLYGEPREGYYGGLEMVHPRIKSGKAAETELPKALTPVYPA
                     GEGIQQRWLRKRIDRALLDLDLTDLVPEAVRTKFGLPGLREALSYIHTPPSGADVIGL
                     QERSAPVWRRLIFDELLAQQITLRESRAVAFQRTAPPLAGDDAELVGCFLAQLPFNLT
                     NAQRRVTDEILADLAANRPMHRLVQGDVGSGKTVVAALAALRAVASGHQASLMAPTEI
                     LAEQHFRKIAAWLEPLGIRTAWLTGRLKTAEKNAALEAVASGEARIVIGTHALIQEGV
                     KFANLGLAIVDEQHRFGVAQRLALRTRPESALVPHLLMLSATPIPRTLAMSYLADLDV
                     SVIDELPPGRTPVVTKTVKTSRKDDVLSVVRSVVAQGRQVYWVCPLIEESDKLDLTPA
                     TVCRDDLQQRLPDLTVGLVHGAMPAAEKDAVMQDFVSGLTQILVATTVIEVGVDVPNA
                     TLMVIEHAERFGLAQLHQLRGRVGRGAAQSACILLFGEDISPAGWERLKAIRDTTDGF
                     EIARRDLEMRGPGEFLGERQSGTPLLRFADLDRDEALLAAARRTADAWLEKDRDAALR
                     HAARWFKTAGEFLAA"
     gene            2310..3254
                     /locus_tag="ACZPB6_08820"
     CDS             2310..3254
                     /locus_tag="ACZPB6_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1184607.1"
                     /translation="MTLTELKYVIAVARERHFGRAAESCFVSQPSLSVAIKKLEEELG
                     VSIFERRTPDISITPVGAAIIEQAQKVLEESRLISEIAKRGQDPLAGPIRLGMIYTVA
                     PYLMPELIVKLSTLAPKMPVYLEETYTTELISQLRTGAIDVAIMADTISDTGFMTQAL
                     YQESFVVAVPSHHPWVNRNYIGPQELRDQTMLLLGAGHCFRDQIIGICPDLVRANIGI
                     ADVQRTVEGSSLQTICHMVAQGLGITVLPASCVPYLRSNVNSIKTIPFKSPSPSRRVI
                     MVWRKSFTRMSAIETLAQAVKSTTLNGCFYLDEPPVAD"
BASE COUNT          571 a         1261 c          948 g          529 t
ORIGIN      
        1 gtggtcaaca aggtgatgac cgaagtcatg tccgagccct atcccgcccg ctcctgcttc
       61 gcggtcgccg cacttcccaa ggccgcccgc gtcgagatcg aggccgtggt gacgatgccg
      121 taaggcaatc cccccatctt tgtatatatg cagaacatgg ccgccgcccc gaaacagcag
      181 gagcgcgcct cgggcgtcat ccgtcaggag aagctcgcgc cccttgacgt caggctcgag
      241 aagctcgggc tcgtgcgcga ctgggacttt gcgcttcacc tcccgcttcg ctatgaggac
      301 gagacgagcg tcacacccat cggctcgctc gccgtgaaca cgcacgccca gatcgagggc
      361 cgcgtcgcag accagcgctt cgtgcgcacg ggccgcggtc agcagcttca ggccgtcgtc
      421 gaggattcga cgggcagcat tacgatccgc ttcctccact acttcccgtc catccagaag
      481 cagcttgccg tcggcagcac cgtgcgcctc tacggcgagc cgcgcgaggg ctactacggc
      541 gggctcgaga tggtgcatcc gcgcatcaag tccggcaagg ctgccgagac cgaactgccc
      601 aaggcgctca cgcccgtcta ccccgcaggc gaaggcatcc agcagcgatg gcttcgcaag
      661 cgcatcgacc gcgcactgct cgatctggac ctcacggacc tcgtgcccga ggcggtcagg
      721 acgaagttcg gccttcccgg ccttcgcgaa gcactctcct acatccacac gccgccctcg
      781 ggcgccgacg tcatcggtct gcaggagcgc tccgcccctg tctggcgcag gctcatcttc
      841 gacgaactcc tcgcccagca gatcacgctg cgcgaatcgc gcgccgtggc ctttcagcgc
      901 accgccccgc cgctcgcggg cgacgacgcc gaactggtcg gctgcttcct tgcgcagctt
      961 cccttcaacc tcacgaacgc ccagcgccgc gtcacggacg agatcctcgc cgatctggcc
     1021 gcgaaccgtc ccatgcaccg cctcgttcag ggcgacgtcg gcagcggcaa gaccgtcgta
     1081 gctgcgctcg ccgccctgcg cgccgtggcc tcgggtcatc aggcctccct catggcgccc
     1141 accgagatcc tcgccgagca gcacttcagg aagatcgccg cctggctcga gcctctgggc
     1201 atccgcaccg cctggctcac gggccgcctc aagaccgcgg agaagaatgc cgcgctcgag
     1261 gccgtcgcct caggcgaagc acgcatcgtc atcggcaccc acgccctcat tcaggaaggc
     1321 gtcaaattcg cgaacctcgg gcttgcgatc gtcgacgagc agcaccgctt cggcgttgcc
     1381 cagcgacttg cgctgcgcac ccgcccagag tccgccctcg tccctcacct cctgatgctc
     1441 tccgcgaccc cgattccgcg cacgctcgcc atgagctacc tcgcggacct cgacgtctcg
     1501 gtcatcgacg agctgccgcc cggccgcacg cccgtcgtca ccaagaccgt gaagacctcc
     1561 cgcaaggacg acgtgctttc cgtcgtgagg agcgtcgttg cgcagggccg ccaggtctac
     1621 tgggtctgtc cgctcatcga ggaaagcgac aagctcgacc tcacacccgc gaccgtctgc
     1681 cgcgacgacc ttcagcagcg cctgcccgac ctcaccgtcg gcctcgtgca cggtgcgatg
     1741 cccgctgccg agaaggatgc cgtcatgcag gacttcgtct cgggcctcac ccagattctc
     1801 gtcgccacca ccgtcatcga ggtgggcgtc gacgttccca atgcaaccct gatggtgatc
     1861 gagcacgcag agcgcttcgg cctcgcgcaa ctccaccagc ttcgcggccg cgtcggccgc
     1921 ggcgccgccc agagcgcctg catcctgctt ttcggcgagg acatctcgcc cgcgggatgg
     1981 gagcgcctca aggcgatcag ggacacgacc gacggcttcg agatcgcgag gcgcgacctc
     2041 gagatgcgcg gccccggaga attcctcggc gaacgccagt cgggcacgcc gctcctacgc
     2101 tttgcggatc tcgatcgcga cgaggcgctc cttgccgccg cacgccgcac ggccgatgcc
     2161 tggctcgaaa aggacaggga tgcggccctc aggcacgcgg cccgctggtt caagacggct
     2221 ggcgaattcc tagccgcatg agccctttgc gaaacgtccc gcaaccgcac taccatattc
     2281 atacaatcca caaacaagga tccctgatca tgactctcac cgaactcaag tacgtcatcg
     2341 cggtggcccg tgaaaggcac ttcggccgcg ccgccgaatc ctgcttcgtg agtcagccct
     2401 ccctttccgt cgccatcaag aagctcgagg aagagctcgg cgtctcgatc ttcgagcgcc
     2461 gcacgcccga catctcgatc acgcccgtgg gcgccgccat catcgagcag gcccagaagg
     2521 tgctcgagga aagccgtctc atctccgaga tcgccaagcg cggtcaggat ccgctcgccg
     2581 gcccgatccg cctcggcatg atctacacgg tcgccccgta cctcatgccc gagctcatcg
     2641 tgaagctctc cacgctcgcc ccgaagatgc ccgtctacct tgaggagacg tacaccaccg
     2701 agctcatctc ccagctgcgc acgggcgcca tcgacgtcgc catcatggcc gacacgatct
     2761 ccgacacggg cttcatgacc caggccctct atcaggaaag cttcgtcgtc gccgttccgt
     2821 cccaccatcc gtgggtcaac cgcaactaca tcggtccgca ggaactgcgc gaccagacga
     2881 tgcttctgct cggcgccggc cactgcttcc gcgaccagat catcggcatc tgcccggacc
     2941 tcgtgcgcgc caacatcggc atcgccgacg tccagcgcac ggtcgagggc tcctcgctgc
     3001 agacgatctg ccacatggtc gcccaggggc tcggcattac cgtgctgccc gcctcctgcg
     3061 tgccctatct gcgctcgaac gtcaacagca tcaagacgat cccgttcaag agcccgtcgc
     3121 cctcgcgccg cgtcatcatg gtctggcgca agagcttcac gcgcatgagc gcgatcgaga
     3181 cgctcgccca ggcggtcaag agcaccacgc tcaacggctg cttctatctt gacgagccgc
     3241 ccgtcgccga ctgagccgca ccgccgaggc catcatgacc caacccgaca cccgaacggg
     3301 cgcggagcc
//
LOCUS       DBMDTI010000226         3303 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6521_length_3303_cov_2.798251, whole genome shotgun sequence.
ACCESSION   DBMDTI010000226 DBMDTI010000000
VERSION     DBMDTI010000226.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6521_length_3303_cov_2.798251"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="ACZPB6_08825"
     CDS             complement(<1..263)
                     /locus_tag="ACZPB6_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HIR1184608.1"
                     /translation="MTIYVWGAIAVVILTLAALIRRYDTKLVLLIAGLAMCCLSMNPM
                     AAFQQFDKSMTNSALIISICSSMGFAACVTMTKCDLHLVSLLT"
     gene            546..1799
                     /gene="pepT"
                     /locus_tag="ACZPB6_08830"
     CDS             546..1799
                     /gene="pepT"
                     /locus_tag="ACZPB6_08830"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIR1184609.1"
                     /translation="MSTVESMSTTERFLQYVAFDTQSVRESTTAPSAEKELALAAFLV
                     DELKALGVEDARIGEGGVVYGSIPATPGCEANPAIGFIAHMDTSPDAPGANARPQIVR
                     YDGGDVTLNVEKNIVFSVGQFPDILKYKGQDVIFTDGTTLLGADDKAGIAEIMQMVAW
                     IKTHPEVPHARLCIGFTPDEEVARGTENFDIPLFGAAYAYTFDGGEIGTLESENFNAG
                     TALVTIRGVGVHPGLSKGKMVNSVRYAARLIDRLPIDQAPETTEGHEGFLHPNMAKGT
                     VVESVVRILVRDHDRALYEEKKAFLVKLVDEMSAEFPEAKFSLEIKDSYENMKPYLDK
                     APKVLEIVRAAYRACGMEPQEEPIRGGTDGARLSVRGLPCPNVFTGGMNFHGVYECIP
                     VKSLDKGAEVAVMLAKKSAEVTSLQ"
     gene            complement(1937..2893)
                     /locus_tag="ACZPB6_08835"
     CDS             complement(1937..2893)
                     /locus_tag="ACZPB6_08835"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1184610.1"
                     /translation="MNVLDNLNAWRAVSAAARTGSVTAAAEQLGIELSKCSRLVSGME
                     REMGFAFFDKNTRPYRPTPQLRAFLAAFEPALRNLENARALHQATGEKMRIRFAAPIE
                     ISRLYFSKALVSYAREHPNLSFVLQPEATLAGLRAGAVDAIVLSDVPAVAEDLVVRLY
                     HVTSTCVLATPAYLRRHGTPQTPADLARHTGLLLRTVNLNPVRRLYRDGIESEILEWK
                     NVFITHDQIALKELLLEDRGIAVDLSLAHVVEELESGRIVSVMPGWERAPWRMCVVTL
                     REREEACPPLRLFAEAVALRTGEDWIAYTERGRAAIARAGTP"
     gene            complement(2903..>3303)
                     /locus_tag="ACZPB6_08840"
     CDS             complement(2903..>3303)
                     /locus_tag="ACZPB6_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1184611.1"
                     /translation="GLLLETVMHPPTQILRKNGAASPILRWKEVFTTHDQMTLRELVL
                     SSLGITVDLYAGHVLPEIRRGRLVPIMPGWRREPWQMSVVTRWEDEARSKALSDFAAY
                     VALEAEATWAPIREETNRVVEEAFRRRLRS"
BASE COUNT          628 a          976 c         1109 g          590 t
ORIGIN      
        1 ttcgtgagca gggaaacgag atgaaggtcg cacttcgtca tcgtgacgca ggcggcaaag
       61 cccatcgacg aacagatcga gatgatgagc gccgagttcg tcatcgactt gtcgaactgc
      121 tggaaggcgg ccatcggatt catcgatagg cagcacatcg ccagacccgc aatcagcagg
      181 accagcttcg tgtcatagcg gcggatgagc gcggcgagcg tgagaatcac gaccgcaatg
      241 gcgccccaga cgtagatcgt cattttgttg tgtctccaga acggttgagg ggcttttctg
      301 aatcctagtg atccgcgcgt ttttgagcca tggggagtta tgaggaaggt ttatgtgatt
      361 acccttacgt atttttgcgg attattttgc aaagacggct ccgaatcttg cgcaggatcg
      421 ggaaattcgc gtttttgtac cactgaggag gtaagggaga acccgcctaa aaaaacggcg
      481 gccgtgtcgg aataatgcac ggcatcagtt tttcgaactc ctttcaaccc tcaggagatt
      541 atccgatgag caccgtcgag agcatgagca cgaccgagcg cttcctgcag tatgttgcgt
      601 ttgacacgca gtccgttcgt gagagcacga ccgcgccgtc cgccgagaag gagcttgcgc
      661 ttgcggcgtt cctcgtcgac gagctcaagg cgctcggcgt cgaggacgcg aggatcggcg
      721 agggcggcgt ggtctacgga tcgattcccg caacgccggg ctgcgaggcc aatcccgcca
      781 tcggcttcat cgcccacatg gacacatctc ccgacgcccc gggcgcgaac gcgaggcctc
      841 agatcgtgcg ctacgacggc ggcgacgtga cgctcaacgt cgagaagaac atcgtcttca
      901 gtgtcgggca gttccccgac atcctgaagt acaagggcca ggacgtgatc ttcacggacg
      961 gcacgacgct tctgggcgcc gacgacaagg cgggcatcgc cgagatcatg cagatggtcg
     1021 cctggatcaa gacccatccc gaggtgccgc atgcgcgcct gtgcatcggc ttcacccccg
     1081 acgaggaagt ggcccgcggc acggaaaact tcgacattcc gctcttcggc gcggcctatg
     1141 cctacacgtt cgacggcggc gaaatcggca cgctcgagag cgagaacttc aacgcgggca
     1201 ccgcgctcgt gaccatcagg ggcgtgggcg tccatcccgg cctctccaag ggcaagatgg
     1261 tcaattccgt gcgctatgcc gccaggctca tcgaccgcct gccgatcgat caggcgcccg
     1321 agacgaccga gggccacgag ggcttcctgc atccgaacat ggcaaagggg acggtggtcg
     1381 agtccgtcgt gcgcattctg gtgcgcgacc acgaccgtgc gctctacgag gagaagaagg
     1441 ccttcctcgt gaagctcgtc gacgagatga gcgccgagtt ccccgaggca aagttctccc
     1501 tcgagatcaa ggacagctac gagaacatga agccctatct cgacaaggcc ccgaaggtgc
     1561 tcgagattgt gcgcgcggcc tatcgcgcct gcggcatgga gccgcaggag gagccgatcc
     1621 gcggcggcac cgacggcgcg cgcctctccg tgcgcgggtt gccctgcccg aacgtgttca
     1681 cgggcggcat gaactttcac ggcgtctatg aatgcattcc cgtgaagtcg ctcgacaagg
     1741 gcgccgaagt cgccgtcatg ctcgcgaaaa agtccgccga ggtgacgtcg cttcagtaag
     1801 catcagcgga ttgcaacagt ttcttccggt ccggatgtga gtctatccgg acaggtggag
     1861 tggggacggg gcccgttcga aagagcgggc cccaattttt tccaccgtgc gagggcggtc
     1921 gtgatccgtc gtttgctcag ggcgtgcctg cgcgtgcaat ggctgcgcgg ccgcgctcgg
     1981 tgtaggcgat ccagtcttcg cccgtcctga gggcgacggc ctccgcaaag agtctcagcg
     2041 gcggacaggc ctcctcgcgt tctctgagcg tgacgacaca catgcgccag ggagcacgct
     2101 cccagccagg catcacggag acgatgcgcc ccgactcgag ctcctcgaca acgtgcgcaa
     2161 gcgagaggtc gacggcgatg cctctgtcct ccaggagaag ttccttgagc gcgatctggt
     2221 cgtgcgtgat gaagacgttc ttccattcga ggatctccga ctcgatcccg tccctgtaga
     2281 ggcgtctcac gggattgagg ttgacggtgc gaagcagcag tcccgtgtgg cgcgcaaggt
     2341 cggcgggtgt ttgcggcgtg ccgtgtcgcc tcaggtatgc gggcgtcgcg aggacgcagg
     2401 tggaggtgac gtggtagagg cgaacgacga gatcctcggc gacggcgggc acgtcgctca
     2461 gaacgatcgc gtcgacggcg ccggcgcgaa gcccggcgag cgtcgcctcg ggctggagca
     2521 cgaacgagag gttcgggtgc tcccgtgcat aggagactag tgccttcgag aagtagaggc
     2581 gggagatctc gatcggagcc gcgaagcgga tgcgcatctt ctcgcccgtc gcctgatgga
     2641 gtgcgcgcgc gttttcaagg ttgcgcagcg cgggctcgaa cgcggcgaga aaggccctca
     2701 gctgcggcgt cggacgatag gggcgcgtgt tcttgtcgaa aaacgcaaag cccatctcgc
     2761 gctccatgcc tgagacgagg cgagagcact tggagagttc gatgccgagc tgctcggcgg
     2821 cagcggtaac gctgcccgtg cgtgcggcgg ctgagacggc gcgccaggcg ttgaggttgt
     2881 cgagaacatt catgacgtgg actcaggacc ggagacggcg gcggaaggct tcttcgacga
     2941 cacggttggt ctcttcacgg atgggcgccc aggtggcctc ggcctcaagc gcaacgtagg
     3001 cggcgaagtc ggagagcgcc ttcgagcgcg cctcgtcctc ccagcgggtg acgacggaca
     3061 tctgccaggg ctcgcgtcgc cagcccggca tgatcggcac gagccggccg cgtcggattt
     3121 caggcaggac gtgtccggca tagaggtcga ccgtgatgcc gagcgaggag aggacgagct
     3181 ccctgagggt catctggtcg tgggttgtga agacctcctt ccagcgcagg atgggagatg
     3241 ccgcgccgtt ctttctcaaa atctgcgtgg gcggatgcat gacggtctca agtagcagtc
     3301 ccg
//
LOCUS       DBMDTI010000227         3297 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6539_length_3297_cov_2.605131, whole genome shotgun sequence.
ACCESSION   DBMDTI010000227 DBMDTI010000000
VERSION     DBMDTI010000227.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6539_length_3297_cov_2.605131"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(567..1832)
                     /gene="dcuC"
                     /locus_tag="ACZPB6_08845"
     CDS             complement(567..1832)
                     /gene="dcuC"
                     /locus_tag="ACZPB6_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HIR1184612.1"
                     /translation="MSIQSWVAIAVTLVTVWALIKRYETRLVLLAAGLFLCCISLDPM
                     YGLDAMGKSMTNKSLIMAICGSMGFAFVASYTQCDRSLVHYLASPIRGLGIFLLPVCT
                     TITFFINIAIPSAAGCAAAVGSTLIPVMLRAGIKPAAAAAAVLGGTIGSYLSPGTSHN
                     VFVANMAKMSEMDFIAYHSTFSLMCGAILIVGIALVAFILGDHKGDKNAQVDESKLQK
                     MDFVPSPLKAIVPFVPIAILVLGNTCLPAIKMGVAQAMLIGAIAALVIARANPQQLTK
                     EFFSGMGKGYADVMGIIIAAGVFAAGLRATGLIDAAVAALKDSNEFARWGGSLGPWIM
                     GVITGSGDAATQAFNEAVTTHAESFGMTIPGLGGLAFLTGALGRTMSPLAGVTILVSG
                     IALVSPIEVIKRTAVPCIVAVVACALLMV"
     gene            complement(2070..>3297)
                     /locus_tag="ACZPB6_08850"
     CDS             complement(2070..>3297)
                     /locus_tag="ACZPB6_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1184613.1"
                     /translation="PHAQSPAAERNRGPQTLRSMLSFSERVDLLIRVVLAVAVSIGCY
                     FIIEPFLTAIVIAAILAVVTWPLFASARSSAGGSTTLPALLMVFLLIIGVLIPSSFLL
                     IALAQQIPKGISVIREWIASGFTIPPAISEIPGIGPWLHDQLLFAIDPASLSTTMQKL
                     LEPLSAALLNAAVNVSNILLQLALVTFIVFFFYRDGAWFADRVQALMKRVSGDLSNEL
                     TKILVNTTRSVVFGLVGTALGQAVVAGIGFWIAGVPGILMLCTLVFVLSIVPIGPPLV
                     WGPSAIWLYSQGETGMAVFLVLWGLLAVSSVDNFLKPILIARGSSLPLALIFLGVFGG
                     VIAFGFLGLILGPILLAVGLSMLQAWLKNPILAAKLDPDRPRRSRRPIKREKRESTQA
                     KGNSLDNPSDQENTSE"
BASE COUNT          701 a          844 c         1209 g          543 t
ORIGIN      
        1 ggattctggg ggggccgtaa ccccccattc tagtccctac ccaattcggg cagtagcggg
       61 ctgcatcaaa cagccctcat cggtttcgtc tagctttttc aagcttctga aagcggacgg
      121 aacgtttgac cgtcggattc ctgcttggtc atgacgttca tgaaggagtc gtgcagatgc
      181 ttgatctcct cgcacgaatt tccttcacga gtctcgtgag ttcctcgccg tgacgatcac
      241 gttgggagga gctgcggcga tgagcgcaag gaagcggggg gcggcgagag aggtgttccg
      301 gttgcagtcg tctgccgtgc cgaggatccc gcgggaaggt gaaggatggg caggcgcgga
      361 cacgcaggaa ggtccttcgc agtctctggg ggcgggagcg ggaggagatg aggtgcgtgg
      421 agcagggggc tgcgggtcgg gcggcgctgc aggacgagag acgagagacg gggagaaaga
      481 gggaaatgaa aagggccgca cctttcggaa cggcccttct tgtctctgtc atgaagctct
      541 cggagaggac ttcaggctct gatgaattag accatcagga gagcgcaggc gacgacagcg
      601 acgatacacg gaacggcggt gcgcttgatg acttcgatcg gggagaccaa ggcgataccg
      661 gacacgagaa tcgtcacgcc ggcgagcggg gacatcgtgc ggccgagagc gccggtcagg
      721 aaggcgagac cgccaagccc cgggatggtc atgccgaagg attcggcgtg cgtggtcaca
      781 gcttcgttga aggcctgggt ggcagcgtcg ccggaaccgg tgatgacgcc catgatccac
      841 ggaccgaggg agccgcccca acgggcgaat tcgttggagt ccttaagggc ggcgacggcg
      901 gcgtcaatga gacccgtggc gcggagaccg gcggcgaaca cgccggcggc gatgatgatg
      961 cccatcacgt cggcatagcc cttgcccatg ccgctgaaga attccttcgt gagctgctgc
     1021 gggttggcgc gggcgatgac gagagcggcg atggcgccga tcagcatggc ctgggcaacg
     1081 cccatcttga tggccggaag gcaggtgttg ccgagcacga ggatggcaat gggcacgaac
     1141 ggaacgatgg ccttgagcgg ggacggaacg aagtccatct tctggagctt ggattcgtcg
     1201 acctgggcgt tcttgtcacc cttgtggtcg ccgaggatga aggcgacgag agcaatgccg
     1261 acgatgagga tggcgccgca catcagggag aacgtggagt gataggcgat gaagtccatt
     1321 tcagacatct tggccatgtt ggcgacgaag acgttgtggg acgtgcccgg agagaggtac
     1381 gaaccgatcg tgccgccgag aacggcagca gcggcggcgg ccggtttgat gccggcgcgg
     1441 agcatcaccg gaatgagcgt cgaaccgacg gcggcagcgc agccggcggc agacggaatg
     1501 gcgatgttga tgaagaacgt gatcgtcgtg cagaccggca gcaggaagat gccgaggccg
     1561 cggatcgggg aggcgaggta gtgaacgaga gaacggtcac actgcgtgta ggaagccacg
     1621 aaggcaaagc ccatcgagcc gcagattgcc atgatgaggg acttgttcgt catggacttg
     1681 cccatggcgt caagaccgta catcgggtca agactaatgc agcagaggaa gagaccggcg
     1741 gcaagaagca ccagtcgcgt ttcatagcgc ttgatcaggg cccagaccgt aacgagcgtc
     1801 acggcaatgg cgacccagct ttgaatcgac ataagaaact ccaaatcctt tggttgttga
     1861 gtgtttttat gggtagggcc gctctgtaac agactccgcc tgagggcggt cctccgaata
     1921 cagttgctag tatgccctac tgtcaaggat cgcgcctgaa ggtttttacg tactgcttca
     1981 ggcaaaaagg agcttcaaaa gggcctgatc gggtcctgaa aaaggaggtt gcagttctgc
     2041 aacctcttga tacgcaacac tttccgtagt tattcggagg tgttttcctg atcagatgga
     2101 ttatctaggg aatttccctt ggcttgagtg ctttcgcgtt tctcccgctt gatcggacgc
     2161 ctgcttctgc ggggacggtc gggatcaagc ttcgcggcga ggatcggatt cttgagccat
     2221 gcctgaagca tggagaggcc gacggcgagc aggatcgggc cgaggatgag gccgaggaag
     2281 ccgaaggcga tcacgccgcc gaagacgccc aggaagatga gggcgagggg cagtgaggag
     2341 ccgcgcgcga tgaggatcgg cttgaggaag ttgtccacgc tcgagacggc gaggagcccc
     2401 cagaggacga ggaagacggc catgcccgtt tcgccctgcg agtagagcca gatggcggac
     2461 gggccccaga cgaggggagg tccgatcggg acgatcgaga ggacgaagac gagtgtgcag
     2521 agcatgagga tgccaggcac gcctgcgatc cagaagccga tgcctgcgac gacggcctgg
     2581 ccgagcgcgg tgcccacgag gccgaagacg acggaacggg tcgtgttgac gaggatcttc
     2641 gtgagttcgt tcgagaggtc gcccgagacg cgcttcatca gggcctgcac gcggtcggcg
     2701 aaccaggcgc cgtcgcggta gaagaagaac acgatgaagg tgacgagggc gagctgcagg
     2761 aggatgttgc tcacgttgac ggctgcattg agaagcgcgg ccgagagcgg ctcgaggagc
     2821 ttctgcatgg tggtcgagag cgacgcgggg tcgatcgcga agagcagctg gtcgtgaagc
     2881 caagggccga tgcccggaat ctcggagatg gcgggcggaa tcgtgaagcc gctggcgatc
     2941 cattcgcgga tgacggagat gcccttgggg atctgctgtg cgagcgcgat gagcaggaag
     3001 ctcgacggga tcagcacgcc gatgatgagc aggaagacca tcaggagcgc gggcagcgtc
     3061 gtggagccgc ccgccgacga acgggcgctc gcgaagagcg gccatgtgac gacggcgaga
     3121 atcgccgcga tgacgatcgc ggtgaggaac ggctcgatga tgaagtagca gccgatcgag
     3181 acggccacgg caagtacgac gcggatgagc aggtcgacgc gctcgctgaa gctgagcatg
     3241 gaacgcagcg tctgcgggcc gcggttgcgt tcggcggcgg gcgactgggc gtggggc
//
LOCUS       DBMDTI010000228         3280 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6585_length_3280_cov_2.236238, whole genome shotgun sequence.
ACCESSION   DBMDTI010000228 DBMDTI010000000
VERSION     DBMDTI010000228.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6585_length_3280_cov_2.236238"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="ACZPB6_08855"
     CDS             complement(<1..485)
                     /locus_tag="ACZPB6_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1184614.1"
                     /translation="MKSTMIKSAVAMVFAAGFAAASMAAPVTAEGTGVGKHGDITVAV
                     TFDAGKIQDIKIVKNAENPILAKKVFTDLKDQVVALSSTDVDLISGATFSAKGFIDAV
                     NDAAKKAGVTLAKADKKALKKAARKLPKTSNYDVVVIGAGGAGFSAAITARNAGANVV
                     LL"
     gene            828..1772
                     /locus_tag="ACZPB6_08860"
     CDS             828..1772
                     /locus_tag="ACZPB6_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1184615.1"
                     /translation="MQQTRSTLPGLLLALVAVFAWGLSYIATEWLYTHDLGPFAVLTF
                     RTFLAYAVLLILSHKQFLADELIDEAKFALLGVACIPFYHGLENLALQETNAGTVATI
                     MASAPLFTAFVVIALHHSFRLHWMTKLGIVTVATGMCLIWFDNHVIQQLPEAGFYLAL
                     GAALAWACYSALLKSVHKYGAVFMARKTFGWGLIAVMPFYLMEDAAPVEALTDPVSAA
                     LLVFLAVGPTTLCTLLWQRAARETGAQQIRNLLFLIPVIAMIAGLVILEESVTFIGVM
                     GAAMILCGVWCSDLGMKRVNAVLAGADADALSAKITLS"
BASE COUNT          633 a         1050 c          953 g          644 t
ORIGIN      
        1 agcaggacga cgttggcgcc ggcgttcctg gcggtgatgg cagcggagaa gcccgcaccg
       61 cccgcaccga tcacgacgac gtcgtagttc gaggtcttcg gcaatttgcg ggcggccttc
      121 ttgagagcct tcttgtcggc cttggcgagc gtcacgccgg ccttcttcgc agcgtcgttg
      181 acggcgtcga tgaagccctt ggcggagaac gtcgcgccgg agatgaggtc gacgtcggtg
      241 gaggagaggg ccacgacctg atccttgagg tccgtgaaga ccttcttggc gagaatcggg
      301 ttttcggcgt tcttgacgat cttgatgtcc tggatcttgc cggcgtcgaa cgtcacggcg
      361 accgtgatgt cgccgtgctt gcccacgccc gtgccctcgg cggtcaccgg agcagccatc
      421 gaggcggctg caaagccggc ggcgaagacc atggcgacgg cggacttgat catggtggat
      481 ttcattgctt tttctcctca aaagcaggag cgccgcaggg tgtccctgcg cccgaaggaa
      541 aaggccttcg gtgcggggcg tcggacggga ggacgctccc attctccata cgaatgtgtg
      601 ttgtcgttca tcggcggcgg accggccgcc agattttcat tctaggaccg tcgcacaggg
      661 acggcaccta aggaatttta cctaggagca tcacctgcag gggcccgccg cgcgggctgc
      721 gccaggcaaa ggaaagtcct cagcccgttg aaaaaacggg gttttccacg cctgcaacga
      781 tatcgggcgc agtagaatgg cggtgtccaa actcctttcg ccctcatatg cagcagactc
      841 gctccacgct accgggcctt cttctcgcgc ttgtcgcggt tttcgcctgg ggcctctcct
      901 acattgcgac cgaatggctc tacacgcacg acctcggtcc gtttgcggtt ctgacgttcc
      961 gcaccttcct cgcctacgcg gtgcttctga ttctctccca caagcagttc cttgccgacg
     1021 agctgatcga cgaggcgaag ttcgcgcttc tcggcgtcgc ctgcattccg ttctaccacg
     1081 ggcttgagaa tctggcgctg caggagacga acgcgggcac cgtcgcgacc atcatggcct
     1141 ccgcgccgct cttcacggcc ttcgtcgtga ttgcgctcca ccacagcttc aggctgcact
     1201 ggatgacgaa gctcgggatc gtgacggtgg cgaccggcat gtgcctcatc tggttcgaca
     1261 accacgtgat ccagcagctt cccgaagcgg gcttttatct ggcgctcggc gccgcgctcg
     1321 cctgggcgtg ctactcggcg ctcctcaaga gcgtgcacaa gtacggcgcg gtcttcatgg
     1381 cccgcaagac cttcggctgg ggtctcattg ccgtcatgcc cttctacctg atggaggacg
     1441 cggctcccgt cgaggcgctc acggatccgg tctcggccgc cctgctcgtc ttcctcgccg
     1501 tgggtcccac gacgctgtgc acgctgctct ggcagcgcgc cgccagggag acgggcgcac
     1561 agcagatcag gaacctcctc ttcctcatcc ccgtcatcgc gatgatcgcg ggcctcgtca
     1621 tcctcgaaga gtccgtcacc ttcatcggcg tgatgggcgc cgcgatgatc ctctgcggcg
     1681 tctggtgctc cgacctcggc atgaagcgcg tcaacgccgt gctcgcgggc gcagacgccg
     1741 atgcgctgag cgcgaagatc acgctctcgt gaccaccttt cccaccgctt ccttcaggcg
     1801 gcgccggagc cacgtgctcc cgcgccgcct tttcattggt tgtctgaaag cccgcgaggc
     1861 cgccctgcag ccctctcgcc cgtcataccc tcatctgcac gatcgtctca cgaaaacgcg
     1921 cgaaggatcg aaaattcaca tgggccgaga cccgaatcac ttgcctgaaa ctctcactta
     1981 acccaaaggg gaccgtgaga aaacaaacgc gcccggcctc cgcccgcccc tccccgttcg
     2041 cacctccgaa acgacgatgt tcgggaggcg tgagctcgac tgctgaggcc cgcaaggccc
     2101 agggcaagca ccggacacct ctcgacggga tcatgctcag gcgccactgc agaggtccgg
     2161 acagggcttc cctggggccc cagggcgcaa ctgtgaacaa cggctcctga gcccatcggg
     2221 atgccccggg acggcatcgg cgagcgcatc gacaccgcct tcagaagccc ctcagaggcg
     2281 cttttttcgc cgtccggcaa aaccgaacga cacttctctt cctctcctgc atcaacggtc
     2341 gtttgattcc cgttcaggtt tttattggtc atctactgca gactccacga aggcggtcct
     2401 ccccgatctc aggaagggag gcgtgcagtg ccagcgtatc aagtcgtgca aaaacgacag
     2461 ccgggctcgg tcgaaaaaag tcccccgaag cgggcgtttt tgaggcaaaa tacgcaccct
     2521 gtgccgccgc tgtacgccgc ttccgacggc tgcttcaagg caccgttaag accccgtcag
     2581 ggtcctaaac gccctgaaaa aggagtccgg accctcaaaa aagaggaaaa aaacgtcttc
     2641 tcaagaagga cgcggatcgt ccgtcacttg caaacgcctc cagcagtagg cgatcaggac
     2701 gggacgcaaa caaacgggat gaatttcgcg caagcgtgct gcgacaggac tcagcaccgt
     2761 tcatcttcgc gcgccttcca aagttaagca tttttgttta aagaggcggt ctcggagggt
     2821 ctgtcgcccc ttggacgaaa ggaggcatga agaagccttc gaaagtccct cccgacgctt
     2881 cgcttttgaa gagagtgacg cagactccct gaaaggggag agcacaatcg tgaaggccgg
     2941 cgcgtcggac acgcgccggc gggttcgcga attttctccg gatcgcgctc aaaaccgcgc
     3001 gagtgaaccg tcggtttttc gccggggagc ctgcaaaaac gaagaatccg cctttcaaaa
     3061 agccttcaaa atgacgcgcg ggttcagaat gacgcccccg aaccacctca aaaatcttgc
     3121 ttaattcaca gcattcgagg gatgaaaggc gtataattcc cattattcga gatgttagag
     3181 cttcggactg aacttcggat tcatgcggaa aagggcccga aaacggcgat tttccgcctt
     3241 ttcggacgaa aagctcaaaa aagaccttaa ctctcttttc
//
LOCUS       DBMDTI010000229         3271 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6610_length_3271_cov_2.642271, whole genome shotgun sequence.
ACCESSION   DBMDTI010000229 DBMDTI010000000
VERSION     DBMDTI010000229.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6610_length_3271_cov_2.642271"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="ACZPB6_08865"
     CDS             <1..457
                     /locus_tag="ACZPB6_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1184616.1"
                     /translation="RYPRGRGPGVEVTAGGGTIPVGRSRTLRKGSRVVFLGFGSMTNV
                     LAPVAEKLDGTLIDMRFVKPVDEEAVLEAARTHDLIVCAEEGMLMGGACNAVLEVLAD
                     NGVVRPVMRFGLPDEFIEQGTQAELLEDVGLTSEAIESKVAERIAKLGI"
     gene            complement(547..1038)
                     /locus_tag="ACZPB6_08870"
     CDS             complement(547..1038)
                     /locus_tag="ACZPB6_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR1184617.1"
                     /translation="MPSFDVEVKADAVELRNAVDQAQKEIGTRYDFRGTKASVEQKDL
                     ELYLVAESDFQLRQVRDVLIGKLAKRGIDVRFLDETKAPEKIGGDCLKQTIVVKEGVT
                     KELGKTIQGIIKNAKALKLTAAIQGDIVRVSGAKRDALQEAIALLKTEVKEVPLNFTN
                     FRA"
     gene            1236..2267
                     /gene="murB"
                     /locus_tag="ACZPB6_08875"
     CDS             1236..2267
                     /gene="murB"
                     /locus_tag="ACZPB6_08875"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR1184618.1"
                     /translation="MISSIQAHRPLDGLTTFGTRSEADHYAEAGSLDELEAHLAEANA
                     NGLPVHFLGAGANTIAMSRVPGMTIRITIKGVEMSAAPNGDVLVKCGAGEVFDDIVAR
                     TLKAGIGGLENLSAIPGTVGGAVVQNIGAYGVELAERLSSVTVYDRAEKVVRVLTVEE
                     CDFSYRHSIMKTEAGRNFVVLSVTLRLPAVWTPVLGYKDLEAEIEARGLTAETVTAPV
                     MSEIVRAVRARKLPDPAVIGNAGSFFTNPIVTKVHWHELLTKHPSLVYYRLGGGRMKL
                     AAAWLIEAAGFKGLAEGPAGVYEHHALIIVNRGGATGEDVMALAERIQKRVFELFGVK
                     LEMEPVRLG"
     gene            2477..3148
                     /gene="trhA"
                     /locus_tag="ACZPB6_08880"
                     /pseudo
     CDS             2477..3148
                     /gene="trhA"
                     /locus_tag="ACZPB6_08880"
                     /inference="COORDINATES: protein motif:HMM:NF011669.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
BASE COUNT          573 a         1006 c         1018 g          674 t
ORIGIN      
        1 ccgctatccg cgcggccgcg gcccaggcgt cgaggtgacg gccgggggcg ggacgattcc
       61 cgtcggccgc tcgcgcaccc tgcgaaaggg cagtcgcgtg gtcttcctcg gcttcggcag
      121 catgacgaac gtgctcgctc ctgttgccga aaagctcgac ggcacgctca tcgacatgcg
      181 cttcgtcaag ccggtcgacg aggaggccgt gcttgaggcc gcgcgtacgc acgatctgat
      241 cgtttgtgcc gaagagggca tgctcatggg aggcgcctgc aacgccgttc tcgaggtgct
      301 tgctgacaac ggagtcgtca ggcccgtgat gcgcttcggc ctgcccgacg agttcatcga
      361 acagggcacg caggccgagc ttcttgagga cgtcggcctc acgtccgagg cgatcgagtc
      421 gaaggtcgcc gagaggattg caaagctcgg catctgagcc ctttccaaag ctgaagaaca
      481 aagaggacgc ccgaacgggc gtcctctttg ttcttgaagc gcaggacgtc gtcctgcagc
      541 ctttcgttac gcgcggaagt tcgtgaagtt gagcggaact tccttcactt cggtcttgag
      601 aagcgcgatg gcctcctgaa gggcgtcgcg cttggcgccc gagacgcgca cgatgtcgcc
      661 ctggatggcg gccgtgagct tgagggcctt ggcgttcttg atgatgccct gaatggtctt
      721 gccgagttcc ttcgtgacgc cttccttcac gacaatggtc tgcttgaggc agtcgccgcc
      781 gatcttttcg ggggccttcg tctcatcaag gaagcgcacg tcgatgccgc gcttggcaag
      841 cttgccgatg agcacgtcgc ggacctgacg gagctggaag tcgctttcgg cgacgaggta
      901 gagttcgagg tccttctgct cgacagaagc cttggtgccg cggaagtcgt agcgggtgcc
      961 gatttccttc tgggcctgat cgactgcgtt gcgaagctcg acggcgtcgg ccttgacttc
     1021 aacgtcaaaa gagggcattt gagtctccaa aagtgtgggc cgcggagcgg ccgttgcggg
     1081 atccgacgtt tctaccgcat cccgccccgc ctgtcaaggc gggatgggac aaaccacggg
     1141 cgcgggtgca tcttgcgtgg gcgtggagca gaccctgcct ataatcaagt cagaactaac
     1201 aacaaggggc ggctcgcgac agccgtcaaa acgccatgat ttcaagcatc caagcccaca
     1261 ggccgctcga cggcctcacc accttcggga cgcgttccga agcggaccac tatgccgagg
     1321 caggctctct cgacgagctt gaggctcatc ttgctgaagc gaacgccaac ggtctgcccg
     1381 tgcactttct cggcgcgggc gccaatacga tcgcgatgag ccgcgtgccg ggcatgacga
     1441 tccgcatcac gatcaagggc gtggagatgt ccgccgctcc caacggcgac gtgctcgtca
     1501 agtgcggcgc gggcgaggtc ttcgacgaca tcgtggcacg caccctcaag gcgggcatcg
     1561 gcggccttga gaacctctcc gccattccgg gcacggtggg cggagccgtc gtgcagaaca
     1621 tcggcgccta cggcgtcgag ctcgctgagc gcctttcctc cgtcacggtc tacgaccgcg
     1681 ccgaaaaggt cgtgcgcgtc ctcaccgtcg aggagtgcga cttcagctac cgccatagca
     1741 tcatgaagac cgaggcgggc cgcaacttcg tggtgctctc cgtaacgctg cgtcttcccg
     1801 ccgtctggac gcccgtgctg ggctacaagg acctcgaggc cgagattgag gcccggggcc
     1861 tcacggccga aaccgtgacc gcgcccgtca tgagtgagat cgtgcgggcc gtgcgcgccc
     1921 gcaagctgcc cgatcccgca gtgatcggga acgcaggctc cttcttcacg aacccgatcg
     1981 tcacgaaggt tcactggcac gagctcctca ccaagcatcc gagcctcgtc tactacaggc
     2041 tcggaggcgg tcgcatgaag ctcgccgccg cctggctcat cgaggccgca ggcttcaagg
     2101 gcttggccga aggtcctgct ggcgtctacg agcaccatgc gctcattatc gtcaaccgag
     2161 gcggcgccac gggcgaggac gtcatggcgc tcgctgaaag gattcagaag cgcgtcttcg
     2221 agctcttcgg cgtgaagctc gagatggagc ccgtgcgtct gggctgaagc ttgaaccgag
     2281 tcttttcgtg atcaatgatc atgcaaaagg tccgcctgcg gacacgttca gcaagcggct
     2341 cttttgcttt cctgtctttg ttcggagcct cctcggtacg aagtgaaata gcgcacggtt
     2401 acaaccctga catttcgttt ccacctttaa tttcgcatga atacaaaatg tatgatttga
     2461 aggaggattt gcaggcatgt ctttcaacag tcctgcggcc gcagtcggct gctattcgac
     2521 aggcgaggag atcgccaatt cggtgacgca cggcgtcgct gcgctgctga gcattgcggg
     2581 gctcgtcgtg atgctcagca tgatgcccgt cacggcgggc gccgccacga tcacggccgc
     2641 cgcagtgttc ggcgcgtcga tgatctttct ctacacggct tcgacgctct accatgccat
     2701 cccgaacctc cgcgtcaagc gcattctgca ggtgcttgac cattcggcga tctatgtcat
     2761 gatcgcgggt tcgtacactc cgttctgtct cgtcacgctc aaggggacga cgggcacgat
     2821 gctctgaatt gcggtctggt cgatcgcgct cgcaggcatc atcctgcagc ccgtactcat
     2881 gaagagggcc gagtggctca actgcctgct ttatctgctt ctcggttggt gcgtcgtgtt
     2941 ggttttcgag ccgctcatgg cggccctgcc ctctgcgggc atctggcttc tggcagcagg
     3001 cggcgtcgtt tactcgctcg gcgtcatttt ctatctctgg gagcggatcc cgtacaacca
     3061 cgccatctgg catctcttcg tgctgggcgg tactgcgctc cagttcttct cggttctctt
     3121 ctacgtgctt cccgcgggcg ccgcctgagt agccgcaggg agagggacga aaaaaaaggt
     3181 tcatctcgga agtccgtgga acgcggcctt gacttttaag aaaaagtaca gatcgtctcg
     3241 gaagccataa gctcgtcgct taatttgctt g
//
LOCUS       DBMDTI010000230         3236 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6698_length_3236_cov_1.767145, whole genome shotgun sequence.
ACCESSION   DBMDTI010000230 DBMDTI010000000
VERSION     DBMDTI010000230.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6698_length_3236_cov_1.767145"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="ACZPB6_08885"
     CDS             complement(<1..993)
                     /locus_tag="ACZPB6_08885"
                     /inference="COORDINATES: protein motif:HMM:NF019348.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1184619.1"
                     /translation="MIPDINLSHLTPATRTVPASVFGEHLAGERDCLGYQVETLPAFA
                     RTRIPVLNPCHFFNEAHYRTLAAWWNLARREPIYVSGPSGSGKTSTAMQFCARLGIPV
                     VSVTARARMDRRELLGHWALQGGETRWIDGPALLAWKHGWTLLINEFSAAPADMWVSC
                     NDILEGLPLDNGATGELVVPHSMTRVIVTDNTRGHSSEIDEGFFGRQIQDRSVIDRFW
                     HMRMEGLGEAEEASLLAATADQDWLAEFEPEVLDRLFRALARLGADSRAAAQAQAIGF
                     ESRAVPLSFRVLSRMRNMMLDAARMPAASDDDPLRRIIRTSLTEALDSTAREAAE"
     gene            1662..2777
                     /locus_tag="ACZPB6_08890"
     CDS             1662..2777
                     /locus_tag="ACZPB6_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR1184620.1"
                     /translation="MFKKSMAALAVLSLSAGLAFASEVTLYGIVDNGLLYKHSKVETN
                     GVSTKTDSFGLESGISSPSRFGIKGSEDLGNGMTVSFKLENGFNADDGSFGDDRLFNR
                     ESSLTLSGDFGKLSFGRMGAIGSAAGDFDAAFGIGDAFDGGDGDVFGMAASDRYDNMV
                     TYQTPSFSGFQATVQYSLNESSVDNTLRENSSQVDRYLGLALTAELGALQGVLAYETQ
                     NYASFGDKAQANTEDGQVVYLGGNYDFEVAKVFVMGQYFKGLAENPFAADAFEGHPVD
                     GEGVKGFGAHVGTIVPVAGGDFTGGLYYVDSTVKYAEEDADGKYYGVALKYEYPLSKR
                     TSVYGGTGWYKAKVDYSADDVYKEEAYQAYLGLTHCF"
BASE COUNT          644 a         1005 c          952 g          635 t
ORIGIN      
        1 ctccgccgct tcgcgagcgg tggagtcgag ggcttcggtc agactggtcc tgatgatcct
       61 gcgaagagga tcatcgtcgg aagctgcggg catccttgcg gcatcgagca tcatgttccg
      121 cattctcgag aggacgcgga agctgagcgg gacggctctg gactcgaacc cgatggcctg
      181 agcctgagcc gcggccctgc tgtcggctcc aagacgcgcg agcgccctga agagcctgtc
      241 gaggacctcg ggctcgaact cggcgagcca gtcctgatcg gccgtcgctg caagcaggga
      301 ggcttcctcg gcttcgccga gaccttccat gcgcatgtgc cagaagcggt cgatgaccga
      361 cctgtcctgg atctgacgac cgaagaaccc ttcgtcaatc tcgctcgaat ggccgcgcgt
      421 gttgtcggtg acgatcacgc gggtcatgga atgcggaacg acgagttcgc cggttgcgcc
      481 gttgtcgagc ggaagcccct caagaatgtc gttgcagctc acccacatgt ctgcgggtgc
      541 tgcgctgaat tcattgatga gaagcgtcca gccgtgcttc caggcgagaa gcgcaggacc
      601 gtcgatccag cgggtctcac cgccctgaag agcccagtga ccgagaagct cgcggcggtc
      661 catgcgggcg cgtgcagtga cgctcaccac gggaatgccg aggcgggcgc agaactgcat
      721 ggccgtactg gtcttgccgg agcccgacgg acccgacacg tagatcggtt cacgacgagc
      781 gaggttccac cacgcggcga gcgtgcggta gtgcgcctcg ttgaagaagt ggcacgggtt
      841 caggacgggg atgcgggtgc gggcgaaagc aggaagcgtt tcgacttggt agcccaggca
      901 gtcgcgttcg ccggcgagat gttcgccgaa gacggaggcc gggaccgtgc gggttgcggg
      961 agtgagatgg gagagattga tgtcgggaat cattttgttt cctttgaaaa aagaaaacga
     1021 ggacgcccga ccccatgtac cccgcatgtg cagggccccg agggcaaacg ccctcggggg
     1081 ggtgaagatg tgacgcgcgc cttcttcaga tgaagaggcg gcggagattg tgcttgcttg
     1141 accggagtct gcagcatgca cgttgcagct gttaagcacc ttcatgatgc accgccccac
     1201 cccgtcttgt caaagggaac ctgaaaattc gccatttccc gtcagaagaa attttcctcg
     1261 gggcgcaggg cgcataaccc cgtcccgaga ggcgccttga gctcaagcgc cgccattggc
     1321 agccctcctg aagcgccatc gtgtggcgcg tttgcaacga aaaaggctct cacaaatacc
     1381 atgtttgcgg tattcggaac gcgtcccgat cccctgtcac gcgtccacat cattgccgtt
     1441 ttcaatgaga acgattgtcg gcaagccttc tcccgcaatg agaatgggtc gcattgtcgt
     1501 ttgcgaacga cagttcgatt cgagcggaaa gtcattggaa gccattctcc acggttcgtg
     1561 atgacgcttg cgcaatgaga atgactcgca ttagagttta agccgacatt ccaatgctgc
     1621 tccctccttc gggagcgcga caaaccatca ggagcatttt catgttcaaa aagtccatgg
     1681 ccgcccttgc ggttctcagc ctttccgcag gccttgcctt cgcctctgaa gtcacgcttt
     1741 acggcatcgt cgacaacggc ctcctctaca agcattccaa ggtcgaaacc aacggcgttt
     1801 cgacgaagac cgactccttc ggtcttgaga gcggcatctc ctccccgtcc cgcttcggca
     1861 tcaagggttc cgaagatctt ggcaacggca tgaccgtgag cttcaagctt gaaaacggct
     1921 tcaacgccga cgacggcagc ttcggcgacg accgcctctt caaccgcgag tcctccctga
     1981 cgctctccgg cgacttcggc aagctctcct tcggccgcat gggcgcgatc ggttccgccg
     2041 ccggcgactt tgacgccgcc ttcggcatcg gcgacgcctt cgacggcggt gacggcgacg
     2101 tcttcggcat ggccgcctcc gaccgctacg acaacatggt tacctaccag acgccgagct
     2161 tctccggctt ccaggccacg gtccagtact cgctcaacga aagcagcgtc gacaacacgc
     2221 ttcgcgaaaa ctcctctcag gtcgaccgct acctcggctt ggcccttact gctgaactcg
     2281 gcgctttgca gggcgtgctc gcctatgaaa cccagaacta cgcctccttc ggtgacaagg
     2341 ctcaggccaa caccgaagac ggtcaggtcg tctacctcgg cggcaactac gacttcgaag
     2401 tcgccaaggt cttcgtgatg ggccagtact tcaagggcct cgctgaaaat ccgttcgcag
     2461 ccgacgcctt cgaaggacat cctgttgacg gtgaaggcgt caagggcttc ggcgctcacg
     2521 tcggcacgat cgtcccggtc gcgggcggcg acttcacggg cggcctctac tacgtcgact
     2581 ccacggtcaa gtacgccgag gaagacgccg acggcaagta ctacggcgtc gccctcaagt
     2641 acgaataccc gctttccaag cgcacctccg tctatggcgg cacgggctgg tacaaggcca
     2701 aggtcgacta ctccgccgac gacgtctaca aggaagaggc ctaccaggcc tacctcggcc
     2761 tcacgcactg cttctgatga ggctcatgca ttgatccttc ggtcagcatg ggaaagattg
     2821 gttccgcgga ccgggattaa gggttgaaaa acaggacggg cggctcccgg tgaggagtcg
     2881 cccgtttcct ttgcgccgat cgacacccat cgagtctcgt cccctatgcg gatgtacggc
     2941 tgggtgtcat ttacaaacaa ctgcaggaga gcgctttaca agacaccctc ccctgccctt
     3001 gaacgtcagt ctcctgggcc ggttgttgca aattagcaac actccgcacg caaaacacat
     3061 atttttacaa ccgagcctga cgtcacgatc gcacctttct gaaggctact cttaccgccc
     3121 ttcgggaata ccctttcttc gcgaaaagaa ggcggcaaac ccttgcgcat caaggggagc
     3181 gctcatttcg tccggatccg tcgaggtatt tccgaaatgc cctgcgtcgc tcgtgg
//
LOCUS       DBMDTI010000231         3201 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6783_length_3201_cov_2.327419, whole genome shotgun sequence.
ACCESSION   DBMDTI010000231 DBMDTI010000000
VERSION     DBMDTI010000231.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6783_length_3201_cov_2.327419"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="ACZPB6_08895"
     CDS             <1..197
                     /locus_tag="ACZPB6_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184621.1"
                     /translation="IVTGRAFPTIKIANDVGCVIAAAALGLVFLGHLYGIGAGTLVSA
                     FAVGLLIKRLKPVLMPGEQL"
     gene            complement(325..906)
                     /locus_tag="ACZPB6_08900"
     CDS             complement(325..906)
                     /locus_tag="ACZPB6_08900"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HIR1184622.1"
                     /translation="MTKQYGFVVDVSRCTGCRTCAVACKDAHNLPVGLNLRRVLEEAS
                     GSWRQDRMTGAWHQDVRAWYVSIACNHCENPACVKVCPTKAHYKRAEDGLVVIDRDKC
                     IGCGMCAQACPYGAPQLDEKARKMMKCDGCLDRLEKGGAPICVEACPQRAISFGEISE
                     LRRTFGTLAVTPPLPDPAQTRPALVIVPSPKAK"
     gene            complement(924..3020)
                     /locus_tag="ACZPB6_08905"
                     /pseudo
     CDS             complement(924..3020)
                     /locus_tag="ACZPB6_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            3088..>3201
                     /locus_tag="ACZPB6_08910"
     CDS             3088..>3201
                     /locus_tag="ACZPB6_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1184623.1"
                     /translation="MVEIVYNRNCVYQTAYHVIWCPKYRKRILKGEIAEALR"
BASE COUNT          554 a          956 c         1049 g          642 t
ORIGIN      
        1 cgatcgtgac gggacgtgcg ttcccgacca tcaagatcgc caacgacgtg ggctgcgtca
       61 tcgcggccgc cgcgctcggc ctcgtctttc tcggacatct ttacggaatc ggcgcgggca
      121 cgctcgtctc cgcctttgcc gtgggcttgc tcatcaagcg cctgaagccc gtgctcatgc
      181 ccggagaaca gctctgaaga cgtgtccctc cggtcccgca tgcctcaaag gatccacgaa
      241 aagacggccc gcctctcgaa tgtgagaagc ggccgttctt cttcaaggga cgccggagtc
      301 cgtctcagtc cctgcgcatc gggatcattt cgccttgggc gacggaacga taacgagtgc
      361 gggacgggtc tgtgcgggat cgggcagggg aggcgtcacg gcgagcgtac cgaacgtcct
      421 tctgagttcg gagatctcgc cgaagcttat cgcgcgctgc ggacaggctt cgacgcagat
      481 gggcgcgccg cccttttcga gacgatcgag gcaaccgtcg cacttcatca tcttgcgggc
      541 cttttcatcg agctgaggcg caccgtaggg gcaggcctgc gcgcacatgc cgcagccgat
      601 gcacttgtcg cggtcgatga cgacgaggcc gtcctcggcg cgcttgtagt gcgccttggt
      661 cgggcagacc ttgacgcatg ccgggttctc gcagtggttg caggcgatcg agacgtacca
      721 ggcgcggacg tcctgatgcc aggcgcccgt catgcggtcc tgacgccagc tgcccgaagc
      781 ttcttcgagc acgcggcgca gattgaggcc gacgggcaga ttgtgcgcgt ccttgcatgc
      841 aacggcgcag gtcctgcagc ccgtgcagcg cgagacgtcg acgacgaaac cgtattgctt
      901 tgtcatgttc tatctccttt tgctcaagcc ttgacgacct cgacgaggtt ggtgtgctgc
      961 ggattgccct tggcgagcgg gctcgggcga agcgacgtga gcgtattgat gttgccgcca
     1021 acgtcgaccc ttcggccgtc tttcatcacg ggcctgtacc acgcgccctg cggaatcgag
     1081 acgacgcccg gaatgatgcg cggcgtcacc ttcgccacaa gttcgaccgc accgcggtca
     1141 ttgaagacgc gcacgcggtc gcccgaacgg atgccgcgag catctgcatc gagcacgttg
     1201 acgagcacct cgtcggggaa ctgctcgcga agtgcgggca ggttgtggaa ggtcgagtga
     1261 atgcggccgt ggccgtgata accgtagcat tggagcggat acttcttctg cagtgcgtcg
     1321 cccggcatct cccaggtgcg cacgaacatc ggaatcggcg aaatgacgtc gcccttggga
     1381 agcacccagt tcttcgcgat gttcgcaagg cgctcggagt agatctcgat cctgcccgac
     1441 ggagttttga gaggattggc cttcgggtcc ttgcggaacg cctcgagcgc gatgcccgcg
     1501 ttgggaagat catggatgtg agcgggcccc gtcttccaga aggtcgcgaa gtcgggaagc
     1561 gacgggatct tgcggcgcgt ttcgttgtag caccattcga tccactcgag ctgggtcttt
     1621 ccttcggtaa aggcctcttc cttgccgaga tgccttgcga tgccgcggca gatctcccag
     1681 gtcgggcgct gctcccactg cggttcgacc gccttgtgaa gcggcaggat ggcggcggag
     1741 tcgccgttgg agccgcctga ggccgtgacg tcgtccgctt cgggtccgag cgtgtccggc
     1801 aggagaatgt cggcgtagcg ggccgacggg gtcatcatgt tgtcgcagac gacgatgaac
     1861 tcgcacttcg aggtgtcgcg caggatttcg tccgtcctgt tcgaatcgct gtgctggttg
     1921 atgagcgtgt tgccgcccga attgacgatc atcttgatgt cgtggccgag cgtcttcaca
     1981 ccgcgcaggc cgtcgtggac gtcatccatc tccctgccgc gcacgatggc atcggtccag
     2041 aggaagcacg gaatcgtcgc cttgacggga ttggtgccga cgggcagata gactgcgggc
     2101 acggccgtgt tgccttcctg ctggccggtg ctcgtgccgg gaaggccgac gttgcccgtg
     2161 aggatcggaa gcatcgcaat ggcacggctc gtctgttcgc cgttggcctg ccgctgcggg
     2221 ccccagccct gtgagaagaa gacgggcttc gtcgtaccga tctcctcggc gagcctcacg
     2281 atcgtctcga cggggacgcc cgtgatgcgg gaggcccatt cgggcgtctt ctcgatgccg
     2341 tcgggccctc tgccgagaat gtggctcttg tagtccgagc ccttgggtgc ggaagcgggc
     2401 agggtgcctt catcgtagcc cacgcaatat tgggcgagga acgcgtggtc cacacggtcg
     2461 tgaacgatga gctcgtgggc aatcgcctcc acgagcgccg cgtcggtgcc gggacggatc
     2521 gggatccatt cgtcggcatg cgcgacggcc gtgtcgctgt agatcggatc gatcacgatc
     2581 atcttcggct tcctgccgcc ctcggcttcg cgcgtgcagg tcatctgcca ggactttccg
     2641 tgtccggagc cgcgcgtgac ggcgggattg tggccgaagg tcacgtagaa cttcgcattg
     2701 gcgatttccg agatgtcaga ggacgggctc gcgccgtagg tcatcgggaa ggcggccgag
     2761 atctgagcag tcgaatagga accgtaatac ttcagataac cgccgcagag attgagcagg
     2821 cgccaccagg ctcggcggga gttgacgagc gactgctagc ccgagcagta ctgccaatag
     2881 agcgattcgt tgccatactt ctcgacggtc tcgcgaagct tgtcggcgac ggtcttgagg
     2941 gcctcgtccc aggagatgcg ctcgaatcgg ccttcgccgc gctcgcccac gcgcttcata
     3001 gaaatgccgc aaggaaacat ctgacttcag tcaggtgagg aattgcggcc catgcctttc
     3061 acactaccac gtgataaaat taaggatatg gtggaaatag tatacaaccg aaattgcgtt
     3121 taccagacgg cataccatgt gatatggtgt ccgaagtacc gcaaacggat acttaaaggt
     3181 gaaatagccg aggctttgcg t
//
LOCUS       DBMDTI010000232         3195 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6805_length_3195_cov_2.180995, whole genome shotgun sequence.
ACCESSION   DBMDTI010000232 DBMDTI010000000
VERSION     DBMDTI010000232.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6805_length_3195_cov_2.180995"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3195)
                     /locus_tag="ACZPB6_08915"
     CDS             complement(<1..>3195)
                     /locus_tag="ACZPB6_08915"
                     /inference="COORDINATES: protein motif:HMM:NF019323.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HIR1184624.1"
                     /translation="GVWRWEARGALAFTPEDPWPERTTFKVDLSGLRLPSATTLNTPV
                     IDFTTPPLTMTSGFATFWMDPAVSGERALTVDALFSTTIADTKAFEASVKVDVPSRSG
                     LTFGKPIFIWNHFRTGFYMKLPVRTLGNATNVRITFPKAAGKWTAADGRHPVVQPGFR
                     QAFVSYTIPAADSLYKMGEASLSPTRTETLEQVYELVLRPSLLTKPSDIVAAMRVTAL
                     PARMNEEAVSPTDWRRAPAVTPDVLARGTPVPVSIAQDPDTPSDSIRLLLKARPGDFL
                     HLALPAGFGPEKGAVLSDGWTTVLAAADPGAAVGFLQPGSMMTLSGSRTLTLMADGVD
                     RIRWRIERIRDPYLALTAQNWRAHETVTNAEALSEAADGVIPVAAGRRFASLPLDEAK
                     LPGGRAGLFQITLTGEKKTKDGWGVAAIAAKRVLVTDTALIAKTSRDGSHVVHAADLV
                     RGVPVAGLRAVLLGANGVPLETVTTDETGAARFATTRGLEREKAPAAVVVTNEATGDL
                     AWLSLSDASNVDHDHDFSTAGRLVSEDGLTGLVFSERGIYRPGETVHAGMIVKTADWH
                     RLPAGMPVTLRITDAAGRTMLETAPALTPEGTASADWTIPTDAVPGEIRIDLLAGDVV
                     LSTHHAAIGDFTPEAMRLTARMVQGAPGWMKPGDLGIDATLAMSYGAPAAKKAMRARL
                     VLSPARDISFPELPGWTFVDPAPFEGTLDEVKVKSVETDAEGRASLTLPLSSTAGTLK
                     GRLLLEGFENGGIRAATENVGFLISPADTMLGWRRHAESTIRKGGVDMPAPEAFSWIT
                     RDEERTFEFLLVDRFLKPCADRPLTLAVMARQSVTELASDSTGRLRYDERSVERPVRE
                     SAVTTDANGRTTASLNTSAPGDFSLVVKDEQGRVLARLPYHVAGEDLRPALSGDLPAA
                     TAKLVSDKSTYEAGEFARLDIVSPFEGLALVTLEADRVLVSKWVKVSAGTNAAQMEVP
                     EGYSGRAFLSASLVRSSKDAARFLKAYARATAPVTLNMKPRTLGVKLEAPAVVPDTRE
                     LKVTVKSDAPGRVFLWAVDTGILSLTGYRTPSPVH"
BASE COUNT          474 a          975 c         1226 g          520 t
ORIGIN      
        1 atgaacggga gacggcgtcc tatagcccgt gagcgacaga atgcccgtgt cgaccgccca
       61 gaggaagacg cgccccggcg catctgactt caccgtgacc ttcagctcgc gcgtgtcggg
      121 caccacggcg ggcgcctcaa gcttcacgcc gagcgtgcgg ggcttcatgt tgagcgtcac
      181 gggcgcggtc gccctggcat aggccttgag gaagcgcgcg gcatccttcg aggagcgcac
      241 gagcgaggcg ctcaggaagg cgcgcccgct gtagccttcg ggcacctcca tctgcgcggc
      301 gttcgtgcct gcgctgacct tcacccactt ggagacgagc actcgatccg cttcaagcgt
      361 cacgagcgcc agaccctcga agggcgacac gatgtcgaga cgggcgaatt cgcccgcttc
      421 ataagtcgac ttgtctgaaa cgagctttgc cgtggcggcg ggcaggtcgc ccgaaagggc
      481 ggggcgcagg tcttcgcctg cgacgtgata gggcaggcgc gcgagcacgc ggccctgttc
      541 gtccttgacg acgagggaga agtcgcctgg cgccgaggtg ttgagcgagg ccgtcgtgcg
      601 gccgttggcg tcggtcgtga cggcggattc cctcacgggg cgctcgacgc tgcgctcgtc
      661 gtagcgcagg cgtcccgtgc tgtcggaggc gagctccgtg acggactggc gtgccatgac
      721 ggcgagggtg agcggcctgt cggcgcaggg cttgaggaag cggtcgacga gcaggaattc
      781 gaacgtgcgc tcctcgtccc tcgtgatcca gctgaaggcc tcgggtgcgg gcatgtcgac
      841 gccgcccttt ctgatggtgg attccgcgtg cctgcgccag ccgagcatgg tgtcggcggg
      901 agagatcaaa aagccgacgt tttccgtggc ggccctgatg ccgccgtttt cgaagccctc
      961 gagcagcaga cggcccttga gcgtgcccgc agtggaggag agcggcagcg tgagggatgc
     1021 gcggccttcg gcgtccgtct cgacggactt caccttcacc tcgtcgagcg tgccctcgaa
     1081 gggcgcgggg tcgacgaagg tccagccggg gagctcgggg aacgagatgt cgcgggcggg
     1141 cgagagaacg aggcgtgcgc gcatggcctt cttcgcagcg ggggcgccgt aggacatggc
     1201 gagcgtggcg tcgatgccga ggtctccggg cttcatccag ccgggcgccc cttggaccat
     1261 gcgggccgta aggcgcatcg cctcgggcgt gaagtcgccg atcgccgcat ggtgggtgga
     1321 gagcacgacg tcgcccgcga gcaggtcgat gcggatttcg cccggcacgg cgtccgtcgg
     1381 aatggtccag tcggccgatg cggtgccttc gggcgtaagt gcgggggcgg tctcgagcat
     1441 cgtgcggccc gcggcgtcgg tgatgcgcag cgtcacgggc atgcctgcgg ggagcctgtg
     1501 ccagtccgcg gtcttcacga tcatgcctgc gtgcacggtt tcgccgggac ggtagatgcc
     1561 gcgctccgag aagacgaggc ccgtgaggcc gtcctcggag acgaggcgcc ctgcggtgct
     1621 gaagtcgtgg tcatggtcga cgttggaggc gtcggagagc gagagccaag cgaggtcgcc
     1681 cgttgcttcg ttcgtgacga cgacggcggc gggcgccttt tcgcgttcga gtccgcgcgt
     1741 cgttgcaaag cgtgcggcgc cggtttcgtc cgtcgtgacg gtttcgagag ggacgccgtt
     1801 ggcaccgagc agcacggcgc gaaggccggc aacgggaacg cccctgacga ggtcggccgc
     1861 atggacgacg tggctgccgt cgcgggaggt cttggcgatg agggccgtgt cggtgacgag
     1921 cacgcgtttg gcggcgatgg ccgcaacgcc ccaaccgtcc ttcgtcttct tctcgcccgt
     1981 gagcgtgatc tggaagagcc ctgcgcgtcc gccgggaagc ttcgcctcgt cgagcgggag
     2041 cgacgcaaag cgccttcccg ccgcaacggg aatgacgccg tcggcagcct cggagagggc
     2101 ctcggcattc gtgacggtct cgtgtgcgcg ccagttctgg gccgtgagcg caaggtaggg
     2161 atccctgatg cgctcgatgc gccagcggat gcggtcgacg ccgtcggcca tgagcgtgag
     2221 ggttctcgag cccgagagcg tcatcatgct gccgggctgc aggaatccga ctgcggcgcc
     2281 ggggtcggcc gccgcaagga cggtcgtcca tccgtcggag agcacggcgc ccttttcggg
     2341 accgaagccc gcgggaagcg ccagatggag gaagtcgccg ggccgggcct tgagaagaag
     2401 gcggatgctg tcggacgggg tgtcggggtc ctgcgcgatc gagacgggga cgggcgtgcc
     2461 gcgcgcgagt acgtcgggcg tgacggcggg tgctctgcgc cagtcggtgg gcgagacggc
     2521 ctcctcgttc atgcgtgcgg gcagagccgt gacgcgcatc gccgcgacga tgtccgaggg
     2581 cttcgtgagg agcgagggcc tgagcacgag ctcgtagacc tgctcgagcg tttcggtgcg
     2641 cgtcggactg agcgaggcct cgcccatctt gtagaggctg tcggcggcag gaatcgtgta
     2701 gctcacgaac gcctgccgga atcccggctg cacgacggga tggcggccgt cagctgccgt
     2761 ccacttgccc gcggccttcg ggaaggtgat gcggacgttg gtcgcgttgc cgagcgtgcg
     2821 cacggggagc ttcatgtaga agcccgtgcg gaaatggttc cagatgaaga tgggcttgcc
     2881 gaacgtgagt ccgcttcttg aggggacgtc gaccttgacc gaggcctcga aggccttcgt
     2941 gtcggcgatg gtcgtcgaga acagggcgtc gaccgtaagg gcgcgctcgc ccgagacggc
     3001 ggggtccatc cagaaggtcg cgaaccccga cgtcatcgtg aggggcggcg tcgtgaagtc
     3061 gatcacgggc gtgttgagcg tcgttgcgga gggcaggcgc aggcccgaga ggtcgacctt
     3121 gaaggttgtg cgctcgggcc agggatcctc gggcgtgaag gcgagtgcgc cccgggcctc
     3181 ccagcgccag acgcc
//
LOCUS       DBMDTI010000233         3191 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6816_length_3191_cov_2.028479, whole genome shotgun sequence.
ACCESSION   DBMDTI010000233 DBMDTI010000000
VERSION     DBMDTI010000233.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6816_length_3191_cov_2.028479"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="ACZPB6_08920"
     CDS             <1..1246
                     /locus_tag="ACZPB6_08920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184625.1"
                     /translation="GSGLAGLSAALTVAEFGYSPVILEKACEPGGLTCRAYGFFNAAD
                     ARRQIALGIDDSPEKHLRQALEVSRGKAREPLMRTLCYEAYSTLQWLEALGLEFEPGI
                     RRVLGGVYPRTHMPLRGAGEAYLSALRSALRPFGVPIVCNAEVTALRKSGDAVTGVEG
                     LLSGEPFRLTSRLGVVLAGGGFSRNMEMMRLYAPGLSDVLPALDEGADGSLIQAAADI
                     GAALVGMSYFEIRLFASTLGLPGDPSDYILVNARGERFMREDRRRSTLAEAVVRQPDG
                     MAWMVINAPGAEPLRPTTAFRETVIATISGYNDCIRTKERDSLGRDPLTMRPIGPGVE
                     IRPVLPRILWSYGGLEINEKAEVIDRRGRPMKGLAAAGDITGGIHGEHALMGDMLASA
                     AVFGRIAARTLVKNGRAVKRQD"
     gene            complement(1375..2571)
                     /locus_tag="ACZPB6_08925"
     CDS             complement(1375..2571)
                     /locus_tag="ACZPB6_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024996.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR1184626.1"
                     /translation="MDPVLCTHGALAAVAMAFAGAAFAGGPGINLPAPALQKGFSIHG
                     LIDTGFQIHHANGTTSARMMPNGDETSAFHLRARENLHGGNYVRLKLSAPFKPDSGEF
                     NNKNTSGDPILFNEAFVAVGGEWGELAAGRLANIFSGNGDFGLCPHINPSPMGTNFPN
                     AGLTPIFSSGYYYNNSVIYNSPRVNGFHVAAMYSNGRDDDSVSRGKSDQFSALALTYI
                     GESFKFAVIPTYIDSDSLKTATVTPNDEYGIALLGSYWPEPTMGLHFGYQFVRDGRAL
                     GGSYFNYFTPAAAGGPGIAKSERGVDTHALSLGFSKRFGAQKISIVLMGNKVEYKGDS
                     AVDGDSEGWRVIPATIYRYYLSKRTHFWASASTSQSGGLYKKAREFEKDPTKAWDVGA
                     GLVHHF"
     gene            complement(2773..>3191)
                     /locus_tag="ACZPB6_08930"
     CDS             complement(2773..>3191)
                     /locus_tag="ACZPB6_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type 2 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR1184627.1"
                     /translation="EAIYHAERKLTEEDLARWPNISVHVPPGSGIDPPAHTDARRNLP
                     GRTQVRSAYILPQPALIAATDLVGIMPEVLIRGAQDAGIAISALSPIFSGMVHRPHLL
                     WHERNDGDTLLRWVMALFVYVFHEEKEGPTFQYPTS"
BASE COUNT          533 a          939 c         1134 g          585 t
ORIGIN      
        1 gggctcggga cttgcgggcc tctccgccgc gctcaccgtc gccgagttcg gctacagtcc
       61 cgtcatcctc gagaaggcct gcgagcccgg cggcctcacc tgccgcgcct acggcttctt
      121 caacgccgcg gacgcccgca ggcagattgc gctcggcatt gacgacagtc ccgagaagca
      181 cctgaggcag gccctcgaag tctcgagggg caaggcccgc gagccgctga tgcgcacgct
      241 ctgctatgag gcgtactcga cgctccagtg gctcgaggcg ctcgggcttg agttcgagcc
      301 cggcatccgc cgcgtactgg gcggcgtcta tccgcgcacg cacatgcccc tgaggggcgc
      361 gggcgaggct tatctgagcg cgctgcgctc cgccctcagg cccttcggcg ttcccatcgt
      421 ctgcaatgct gaggtgacgg cgctcagaaa gtcgggcgac gccgtgacgg gcgttgaggg
      481 gctgctttcg ggcgagccct tcaggctcac ctcgaggctc ggcgtcgtgc tcgcgggcgg
      541 cggcttctct cggaacatgg agatgatgcg gctttatgcg ccgggcctct ccgacgttct
      601 ccccgccctt gacgaaggtg cggacgggag tctcattcag gccgctgccg acatcggcgc
      661 ggcgctcgtg ggcatgtcct atttcgagat ccgactcttt gcgtccacgc tcggtcttcc
      721 gggggatccg tccgactaca tcctcgtcaa cgcgcgaggc gaacgcttca tgcgcgagga
      781 ccgtcgccga tccacgctcg ccgaggccgt cgtcagacag ccggacggca tggcctggat
      841 ggtgatcaat gcaccgggcg ccgaacccct caggcccacg acggccttca gggagacggt
      901 catcgcgacg atctcgggtt acaacgactg cattcgtacg aaggaaaggg attccctcgg
      961 acgcgatccc ctcacgatga ggccgatcgg tccgggcgtc gagatcaggc ccgttctgcc
     1021 gagaatcctc tggtcctacg gcggtctcga gatcaacgag aaggccgagg tgatcgaccg
     1081 caggggcaga cccatgaagg gacttgcggc cgcgggcgac attacgggcg gcattcacgg
     1141 cgaacacgcc ctcatgggcg acatgctcgc gtcggctgcg gttttcggac gaatcgcggc
     1201 gagaacactc gtgaagaacg gcagggccgt caagcgtcag gactgaggga cggacggcga
     1261 agaaacgcag gaagaagggc acggctcgaa tcgagtccgc gcccttgtca gtgtgctcgg
     1321 catgggcacg ttctaagccg ggagcaggcc cgggcttcgg agagacgctc gggatcagaa
     1381 gtggtgcacg agccccgcgc ctacgtccca ggccttcgtc ggatcctttt cgaattcgcg
     1441 ggccttcttg tagagaccgc cgctctggga ggtgcttgcg gacgcccaga agtgcgtgcg
     1501 cttgctcagg tagtagcggt agatcgtcgc gggaatgacg cgccaacctt cgctgtcgcc
     1561 gtcgacggcg ctgtcgccct tgtattcgac cttgttgccc atgaggacga tcgagatctt
     1621 ctgggcgccg aagcgcttgg agaagccgag cgagagcgcg tgcgtgtcga cgccgcgttc
     1681 ggacttggcg atgcccgggc cgccggcggc ggccggcgtg aagtagttga agtagctgcc
     1741 gccgagcgcg cggccgtcac gaacgaactg atagccgaag tgcaggccca tcgtgggttc
     1801 gggccagtag ctgcccagaa gcgcgatgcc gtattcgtca ttaggtgtga cggtcgccgt
     1861 cttcagcgag tcgctgtcga tgtaggtcgg aatgaccgcg aacttgaagc tctcgccgat
     1921 gtaggtgagg gcgagcgcgc tgaactggtc gctcttgccg cggctcacgg agtcgtcgtc
     1981 acggccgttc gaatacatgg cggcgacgtg gaagccgttg acgcggggcg agttgtagat
     2041 gaccgaattg ttgtagtagt agcccgagga gaagatcggc gtgaggccgg cgttcgggaa
     2101 gttcgtgccc atgggcgacg ggttgatgtg cgggcacaga ccgaagtcgc cgttgcccga
     2161 gaagatgttc gcaagacgtc ctgcggcgag ctcgccccat tcgccgccca cggcgacgaa
     2221 ggcttcgttg aagagaatcg gatccccgga cgtgttcttg ttgttgaatt cgccgctgtc
     2281 gggcttgaag ggcgcggaga gcttcagacg gacgtagttg ccgccgtgga ggttttcacg
     2341 ggcgcgcagg tggaaggccg aggtttcgtc accgttgggc atcatgcggg cgctcgtggt
     2401 gccgttggcg tgatggatct ggaagccggt gtcgatgagg ccgtggatgc tgaagccttt
     2461 ctggagggcg ggagcgggga ggttgatgcc ggggccgccg gcgaaggccg cgccggcgaa
     2521 agccatggcg acggcggcaa gggcgccgtg ggtgcagaga acggggtcca tgatggtcta
     2581 atccttgtgt gttggttggg tcgcaaaaac gagggggcgc ctgcgtttgc aggcgacctg
     2641 agtttggtcg gccgtttttg cgacaaaacg aagattagag aatgctgtgg gccctgtctc
     2701 ttgtgaggat tcccgtgccg attgtctgaa agcgatgttg tgtttctcga aaatcggcaa
     2761 atcctcggga aactacgaag tcggatattg gaaggtcgga ccctcctttt cctcatgaaa
     2821 gacgtacacg aagagtgcca tcacccagcg aagcaacgtg tcgccgtcgt tgcgttcgtg
     2881 ccagagaagg tgcggcctgt gcaccatgcc cgagaagatg ggcgagagcg ccgaaatggc
     2941 gatgcccgcg tcctgcgcgc cgcggatcag gacttcgggc atgatgccca cgaggtccgt
     3001 cgccgcaatg agcgcgggct gaggcaggat gtaggccgag cggacctgcg tgcggccggg
     3061 caggttgcgg cgcgcgtcgg tgtgagccgg cgggtcgatc ccgctcccgg gcggaacgtg
     3121 cacgctgatg ttgggccagc gcgcgaggtc ctcctcggtg agcttccttt cggcgtggta
     3181 gatcgcctcg a
//
LOCUS       DBMDTI010000234         3175 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6850_length_3175_cov_2.124593, whole genome shotgun sequence.
ACCESSION   DBMDTI010000234 DBMDTI010000000
VERSION     DBMDTI010000234.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6850_length_3175_cov_2.124593"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            358..2133
                     /locus_tag="ACZPB6_08935"
     CDS             358..2133
                     /locus_tag="ACZPB6_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR1184628.1"
                     /translation="MKNVEPHWQPDGLLNIASERYAKRKETLADVLLLAERLSADNPS
                     DAAAVERLIELVEHGRRLAVSLLTACKATQTQQAKNSRVPAERTDLLHATERLEALAE
                     RARNWGRVFPKDLEGRPAGEWLEFEEAAQSRVILRPTEADRRIEEPLGALYTHLKSSM
                     KIMVQMPDGTHPTVMSCSSAESYLKNSTDTATRRQVFGAYNAWFAENAAPFAALLNAM
                     TGHRLNLSDRAGMSMLDASLKSERMSRPAFDAMMRAVRAAAPRARNAVQLSGMVLSCL
                     EGRRSGLSVANLFAPLPEAAPLEAAASFDGATDALLRTFSRIDPDFQGFLKRLLSAGW
                     IETSNISGGEGGTWCAQVPACSAVAVYADYQPTLNRACELAHILGEASLRDRLIAYPA
                     IAQNPSLLSCEIAGRFFMDAFLAQLLEETEWERREHAAVLWQALRQIGVNLLHLPFRF
                     ELSHRLHEERRHGYLSVNDINKLTNTIWEDYFADTVRGSDQYVWAAKLHFYLPREPHY
                     DWQYTAGYLIAAVLRRRLLADGRTACGGSFGDFLSDCATIDCDELIRRHLGADPASDA
                     FWTDAATEALNVMRDADRHIQSFIS"
     gene            complement(2268..>3175)
                     /gene="dcuC"
                     /locus_tag="ACZPB6_08940"
     CDS             complement(2268..>3175)
                     /gene="dcuC"
                     /locus_tag="ACZPB6_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HIR1184629.1"
                     /translation="VGLMIRAGFRPAMAAAVIISSTLPNYWSPGSTDNIYVAKLANIP
                     VMDMVTYVAPTTLILSALSIVFVMIVCLLFKDYRKEGFGSTGVHPGEDIQKKLPDLPE
                     KPNLLMAFAPLLPVVLLFVISLCFPGVKMSVATAMLMGLIYVMIVTRLNPQQLCSKFF
                     DGMGSGYGSILGLIIAAGVFAAGLKSCGLISLFIDYLKNSSDVAKLGASFGPYLLGII
                     TGSGNAAAFSFNESVTPHALEFGMKIEDLGFIACVSATLGRVSSPLAAGVILIAGIAG
                     ASPLDVVKRSIPVMLCTIGALYFLV"
BASE COUNT          611 a          995 c         1057 g          512 t
ORIGIN      
        1 tacgagctcg tctacaagac ggcgagctac cgcatcggcg gcaagccccg tcccgaacgc
       61 tcctgggagg tgatgagcac cgagatcgcc gaggcgatca agcgcgcgtg acgctctgat
      121 gaagcctcag gttcaaggag gggacgttcc gaagggaacg tctctttttt ttgtcttcgg
      181 gaaaagcgtg ttttcggcgt ccgaaggcgc ctcccgggac gttctgaaga gacggctgcc
      241 cgcttgcgcc tgtgccagag gtgttgagcg ggatcaaggg ggcaagcttg cctttgtcgg
      301 aggtgcggtt acaatattcc gtctgattcg atgaccacgt cagaagaggg gaagaaattg
      361 aagaacgtgg aaccgcactg gcagcccgac gggctcctca acattgcgag cgaacgctac
      421 gccaagcgaa aggagacgct ggccgacgtg ctgcttcttg ccgaaaggct ctctgcggac
      481 aatccgtccg atgcggccgc cgtcgagcgc ctcatcgagc tcgtcgagca cggccgcagg
      541 cttgcggtct cgcttctgac cgcctgcaag gcgacgcaga cccagcaggc caagaacagc
      601 cgcgtgcccg ccgagaggac ggacctgctt catgcaacgg agcgcctcga ggcgcttgcc
      661 gaacgcgccc gcaactgggg ccgcgtcttc ccgaaggatc tcgagggcag gcccgcgggt
      721 gagtggctcg aattcgagga ggccgcccag agccgcgtga tcctgcgccc gaccgaggcc
      781 gaccgccgca tcgaggagcc gctcggcgcc ctctacaccc atctcaagag cagcatgaag
      841 atcatggtgc agatgcccga cggcacgcat ccgacggtca tgagctgctc gtcggccgaa
      901 tcctatctga agaacagcac cgacacggcc acgcgccgcc aggtcttcgg cgcctacaat
      961 gcgtggttcg ccgagaacgc cgcgcccttt gccgcgctgc tcaacgccat gacgggccac
     1021 aggctaaacc tctcagaccg cgcgggcatg tcgatgctcg acgcgtccct caagagcgag
     1081 cgcatgagcc gccccgcctt cgacgcgatg atgagggccg tgcgcgctgc tgcgccgagg
     1141 gcccgcaacg ctgtccagct ctcgggcatg gtgctctcgt gcctcgaggg ccgcaggtcg
     1201 ggcctttcgg tcgcgaacct ctttgccccg cttcccgaag ccgcgcctct tgaagcggcc
     1261 gcctcgttcg acggcgccac cgacgcgctg cttcgcacct tcagcaggat cgatcccgac
     1321 tttcagggct ttctcaagcg cctcctttcg gcaggctgga tcgagacgag caacatctcg
     1381 ggcggcgagg gcggcacgtg gtgcgcgcag gtgcccgcct gcagcgccgt ggcggtctat
     1441 gcggactacc agcccacgct caaccgcgcc tgcgagcttg cccacatcct gggcgaagcg
     1501 tccctgaggg acaggctcat cgcctatccc gccattgcgc agaatccgtc ccttctttcg
     1561 tgcgaaatcg cgggacgctt cttcatggac gccttcctcg ctcagcttct cgaggagacg
     1621 gagtgggagc gccgcgaaca tgcggccgta ctctggcagg ccctgcgcca gatcggcgtc
     1681 aacctcctgc acctgccctt caggtttgaa ctttcgcaca ggctccacga ggagagaagg
     1741 cacggctacc tctcggtgaa cgacatcaac aagctcacca acaccatctg ggaggattat
     1801 ttcgcggaca cggtccgagg gtccgaccag tacgtctggg ccgccaagct tcatttctat
     1861 ctgccgcgcg agccccacta cgactggcag tacacggcgg gctacctcat cgcggccgtt
     1921 ctgaggcgca ggcttctggc cgacggccgc acggcctgcg gcggctcgtt cggggacttc
     1981 ctctccgact gcgcgacgat cgactgcgac gagctcatca gacgccacct cggcgccgat
     2041 cccgcctccg acgccttctg gacggacgct gccacggagg cgctcaacgt catgcgcgat
     2101 gcagaccgcc acatccagtc cttcatctcc tgagaaggga agggccggaa aaggataaaa
     2161 aggcaaaaaa caaaaaggaa agccccgtgc ggaggaccgc ccggggcttt tttctgactt
     2221 cagacctcgg gcgcagcgag gcgcgcccga aggagagaag gcattcgtca gacgaggaag
     2281 tagagcgcgc cgatcgtgca gagcatgacg gggatggagc gcttgacgac gtcgagcgga
     2341 gaggcgcctg cgatgcctgc gatcaggatg acgccggcgg cgagcggaga cgagacgcgg
     2401 ccgagcgtgg cggagacgca ggcgatgaag ccgaggtcct cgatcttcat gccgaactcg
     2461 agggcgtgcg gcgtgacgga ttcgttgaag gagaacgcgg cggcgttgcc cgagcccgtg
     2521 atgatgccga gcaggtaggg gccgaaggag gcgccgagct tggcgacgtc ggaggagttc
     2581 ttcaggtagt cgatgaagag gctgatgaga ccgcaactct tgaggccggc ggcgaagacg
     2641 cccgcggcga tgatgaggcc gaggatgctg ccgtagcccg agcccatgcc gtcgaagaac
     2701 ttcgagcaga gctgctgcgg gttgaggcgc gtgacgatca tcacgtagat gaggcccatg
     2761 agcatggcgg tggcgactga catcttcacg ccggggaagc acagcgagat gacgaagagg
     2821 agcacgaccg gcaggagcgg cgcaaaggcc atcagcaggt tcggcttttc aggcaggtcg
     2881 gggagcttct tctggatgtc ttcgccagga tgcacgcccg tcgagccgaa gccctccttg
     2941 cggtagtcct tgaagagcag gcagacgatc attacgaaga cgatggagag cgccgagagg
     3001 atcagcgtcg tcggggcgac gtacgtgacc atgtccatca cgggaatgtt ggcgagcttg
     3061 gcgacgtaga tgttgtcggt ggagcccgga gaccagtagt tcgggagcgt cgatgagatg
     3121 atcacggcgg cggccatggc gggacggaag cccgcgcgga tcatcagacc cacga
//
LOCUS       DBMDTI010000235         3170 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6862_length_3170_cov_1.798631, whole genome shotgun sequence.
ACCESSION   DBMDTI010000235 DBMDTI010000000
VERSION     DBMDTI010000235.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6862_length_3170_cov_1.798631"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(167..1138)
                     /locus_tag="ACZPB6_08945"
     CDS             complement(167..1138)
                     /locus_tag="ACZPB6_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR1184630.1"
                     /translation="MDRRRLLQLAGAFLAAGSAPGALFAQTTQMTMVVPFPPGGGGDI
                     LARTVSLPFSEALRENVVIFNRPGAGGNIGTGQVVKSQPDGTTIGYVTNGIMCVNPYL
                     YDRQSFDPLKDLTPVAGLTSIGLVLAVSPKALPDVHTAADLVKHAQARPGETAFASAG
                     PGTTSHLAGILLESEAKLDLNHIPHAGGAAAVKEVLAGRVPFLIDVMPNLLPHLKAGT
                     LRALAVTTPSRSALLPKVPTMAEAGLPGVTLYAWDGIAAPKGLSDADADRFAKAARAA
                     LNDKAVRDRLLRMGAEPQFRGPADFRQFIEAEAAKWRPLALMARAQR"
     gene            1766..>3170
                     /locus_tag="ACZPB6_08950"
     CDS             1766..>3170
                     /locus_tag="ACZPB6_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HIR1184631.1"
                     /translation="MATGVYAGKGGKIVIGDRDKSVVTIRTKDTGVVKDSLSYGLWVN
                     GQENLGGQMEVKGKSVTIHAEGEGQTDVRAIHVATNTLKPSQRSSLLIDADTVSITAD
                     GEGARAIVAMSTGVVEVKGKATIAADNAIVARGDAKVLVNAEGKYSTVINGNVNFSYD
                     APTSKTPVDATVILNLVGPESSWRGNTVVSWNGKPTSVDEYLSVTGMKLGLSKGAAWT
                     PVETAKDSTTANVGAKYTALNLLENNDGVINITGSAIDVTVEKMTGTGGTVNLAADLT
                     AEEGSRTGTITIAEADENSKIDVKLKDAKMERNLTSDDLTAEEAKSLMAAGVDAAENV
                     GVTSTVEEGMYNDGFRIDEEGATTSTGPNSVMQSTLELAAAAPLAINRILMNDVRKRL
                     GDIRTSQGTSGVWARYDGGRLSGSAGLENDFHTIQAGVDTVPGDSSIRFGAALSYTGS
                     EADYRRGNADMDLYGLSA"
BASE COUNT          660 a          833 c         1076 g          601 t
ORIGIN      
        1 ggacggttga tcgtgatctt ggcgatgccg tccgagcact tctcatagac gatgtcggtg
       61 aattcgcccg ctttgtgcca ttcgggaacg ggaggaagat gtgaggtgtc gagcatgggc
      121 tgcatgtcgt taattccttt tcaaacagtc tgtatgggtt cggcctttag cgctgtgcgc
      181 gggccatgag ggcaagcggg cgccacttgg cggcctcggc ctctatgaat tgcctgaagt
      241 cggcgggacc gcggaactgg ggttcggcgc ccatgcgcag aagacggtcg cgcacggcct
      301 tgtcgttgag cgcggcacgg gcggccttcg cgaagcgatc ggcatcggcg tcggagagcc
      361 ccttcggggc ggcgatgccg tcccaggcgt agagcgtgac gccgggaaga cctgcttcgg
      421 ccatggtggg aactttgggc agaagtgcgg atcgtgaggg cgtcgtgacg gcgagggcgc
      481 gaagcgtgcc ggccttgagg tgcggaagga ggttgggcat cacgtcgatg aggaagggca
      541 cacggcctgc gagcacttcc ttgacggcgg cagcgccgcc cgcgtgcgga atgtgattga
      601 ggtcgagctt tgcttcgctc tcgagcagaa tgcccgcgag atggctcgtg gtgccggggc
      661 ctgcggacgc aaaggcggtt tcgccggggc gggcctgggc gtgcttcacg aggtcggctg
      721 cggtgtgcac gtcgggaagg gccttggggc tgacggcgag aaccagcccg atcgaggtga
      781 ggccggcgac gggtgttagg tccttgagcg gatcgaagct ctgccggtcg tagaggtacg
      841 gattgacgca catgatgccg ttcgtgacgt atccgatcgt cgtgccgtcc ggctgggact
      901 tgacgacctg gccggtgccg atgttgccgc ccgcaccggg acggttgaag atcaccacgt
      961 tttcacggag cgcctccgag aagggaaggg ataccgtgcg cgcgaggatg tccccgccgc
     1021 ctcctggcgg gaaggggacg accatcgtca tctgcgtcgt ctgcgcaaag agggcgcctg
     1081 gtgccgatcc cgcggcgaga aaagcccctg cgagctgaag aagtctgcgt cggtccatgt
     1141 ttttctccgt ttgagtgcct tgaatggtac cgtatggtac taacgtactg catgatacag
     1201 atgcatcctg aaatgcgcaa atggcaggcg ttgttcctgt gactgcatgg ggaatggggc
     1261 cgtgggtgca gagggatgct tcagtttgga gatgacggat tttgggtatg ggatttgtaa
     1321 ctgaggagtc ggtctgtgat aggccgaatt cgaggcttca gtgaattcgg aagaaattcg
     1381 gcgcatcacg attccaatgg attgaggtga tttcctgtag taaagtgatt ggcagaaatg
     1441 ctttctccgt ctgctggaaa gggcagatga acttgaggca ccgaagtgga tcggaaagcc
     1501 atgaacatca gattgacgtg tccgttggga gcgtcgagga gggcttatga agcgcacgat
     1561 tttgtcagtg gccgttctgg tcgcatgtgc atctcaggca tcagccagcc ttacggctcg
     1621 gtaccttctt gaaggtgaca agacctttgc agaagacatc acttgcgcag tggcgagtaa
     1681 cttcggcatc tacgcacatg gttcaggaaa cgcgggaacg ttcactggtc aaaccatctc
     1741 agtggacgtt tccgaagcgg ccggtttggc aacgggtgtc tacgcaggca agggcggcaa
     1801 gatcgtcatc ggtgatcgtg acaagagcgt cgtgacgatc agaaccaaag atacgggcgt
     1861 cgtcaaggac agcctgtcct atgggctctg ggttaacggt caggagaatc tgggcgggca
     1921 gatggaagtc aagggcaaaa gcgtcacgat tcatgccgag ggtgaaggac aaacagacgt
     1981 gcgagccatt catgtggcga cgaacacgtt gaaaccctct caaaggtctt cgctcctcat
     2041 tgatgccgac accgtcagca ttacggctga tggtgaagga gctagggcaa tcgtagcgat
     2101 gagcacgggt gttgtcgaag tcaaaggcaa ggcgacgatt gcggcggaca atgccatcgt
     2161 tgcgcgcggg gacgcgaagg tgctggtcaa cgcggaaggg aagtactcga ccgtcatcaa
     2221 cggcaacgtc aacttcagct acgacgctcc gacctccaag acgccagtcg acgccacggt
     2281 gattctgaat ctcgtcggcc ccgaatcaag ttggaggggc aacacggtcg tctcctggaa
     2341 tggcaagcca acatcggtcg acgagtatct ctcggtcacg ggcatgaagc tcggccttag
     2401 caaaggcgcg gcgtggacac ccgtcgaaac agcgaaagat tcgacgaccg ccaacgtcgg
     2461 cgcgaaatac acggccctca accttctcga gaacaatgac ggcgtgatca acatcacggg
     2521 cagcgccatc gacgtgacgg tcgagaagat gaccggtacg ggcggtaccg tcaatctcgc
     2581 tgcggatctg acggctgagg aaggaagccg gaccggcacg atcaccatcg ctgaagccga
     2641 cgaaaactcg aagatcgatg tcaagctcaa ggacgcgaag atggagagga acctcacctc
     2701 cgacgacctg acggcggaag aggcgaagtc gctcatggcg gcgggtgttg atgcagccga
     2761 gaacgtcggc gtcacgagta ctgtcgagga aggcatgtac aacgacggct tccggattga
     2821 cgaggaaggt gccacgacga gcacgggacc gaactcggtc atgcagtcaa cgcttgagct
     2881 cgccgccgct gcgccgcttg cgatcaacag gatccttatg aacgacgtga gaaagcgtct
     2941 cggtgacatc cgcacgtctc agggtacgtc tggcgtctgg gcacgctatg acggcggtcg
     3001 cctctcgggc tccgctggcc ttgaaaacga cttccacacg atccaggcgg gcgtcgacac
     3061 ggtgcccggc gacagctcga tccgcttcgg cgcagcgctg agctatacgg gcagcgaagc
     3121 cgactacagg cgcggcaatg ccgacatgga cctctatggt ctttcggcct
//
LOCUS       DBMDTI010000236         3148 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6910_length_3148_cov_3.423039, whole genome shotgun sequence.
ACCESSION   DBMDTI010000236 DBMDTI010000000
VERSION     DBMDTI010000236.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6910_length_3148_cov_3.423039"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="ACZPB6_08955"
     CDS             <1..688
                     /locus_tag="ACZPB6_08955"
                     /inference="COORDINATES: protein motif:HMM:NF025910.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1184632.1"
                     /translation="SLTDGELAPQARLREAAALAESQRFDDARAVLQGAAEDYPEHLN
                     LFMTAEARMLIEAGRPEDALGVMKTALAEQPDDVSLAYDTAMIAQNVGHFEETESILK
                     DILSDDPDHVQAGNALAYLWVTRDMNLPEARRLLEHAYRLSPVDPYVLDSMGWLCFKE
                     GRYDQAAEFTLASLKRLFDVEVACHLVEILAVSGRDHEAEKLFRELEQRAGSDALVLD
                     LAKRLNYKTK"
     gene            685..1182
                     /locus_tag="ACZPB6_08960"
     CDS             685..1182
                     /locus_tag="ACZPB6_08960"
                     /inference="COORDINATES: protein motif:HMM:NF015512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HIR1184633.1"
                     /translation="MKRRILLALPAAAAVLTLAGCVTGQPPLFERYGRFALVSTSASG
                     EREAVNGRFSLRRTSAGLRLNLMTPLNGILASIEVTPSGAVLETSQNESPLRAATAEE
                     LMLRTTGVAVPVETLERWLTMKGDRASEYGWSVRVLKRRDDGMPGTIRASSTGVSLTL
                     TMDED"
     gene            1184..2050
                     /gene="ispE"
                     /locus_tag="ACZPB6_08965"
     CDS             1184..2050
                     /gene="ispE"
                     /locus_tag="ACZPB6_08965"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR1184634.1"
                     /translation="MLSDSALLGLPAPAKLNLFLHVVGRRPDGKHLLESVFTLIDLHD
                     TIDLFRLEEPVVERTGDVIGNVEKDLCVRAARLLMETFDVRSGVRIHVEKRIPAGAGM
                     GGGSSDAATVLIGLNRLFGLGLSRSALMELGERLGADVPFFIFGRTGFVEGIGEIITP
                     LEIPPASYAVIWPGRGISTAEIFAAPNLTRDTKSLKIDILSGKALEIWPNLYGHNDLQ
                     NVAQELEHRVTEGLGMLACGTHDARMTGSGSAVFGVIADPCSYRLPQLPDGWKGFAAK
                     SLREHPLSAWLD"
     gene            2062..2139
                     /locus_tag="ACZPB6_08970"
     tRNA            2062..2139
                     /locus_tag="ACZPB6_08970"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2097..2099,aa:Gln,seq:ttg)
     gene            2286..>3148
                     /locus_tag="ACZPB6_08975"
     CDS             2286..>3148
                     /locus_tag="ACZPB6_08975"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008110854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR1184635.1"
                     /translation="MVPESMMVFTGNANPKLAQAVTKYLNIPLGSATVNRFSDGEVMV
                     QLLDNVRGKDVFILQSTCAPTNDNLMELMIMVDALKRASARRITAAIPYYGYARQDRR
                     PRSTRVAISAKVVANMLQAVGVDRVLTMDLHADQIQGFFDIPVDNIYATPVLLNDLVE
                     RKYDDLMVVSPDVGGVVRARAMAKALEVDLAIIDKRRPRANVAEIMNIIGEVKGRTCV
                     ITDDIVDTAGTLCNAAGALKERGAKRVVAYITHPVLSGAAVERIMNSALDELVVTDTI
                     PLSAAAEACPK"
BASE COUNT          597 a         1102 c          895 g          554 t
ORIGIN      
        1 ctccctcacc gacggcgagc ttgcgcccca ggccagactt cgcgaggccg ccgcgcttgc
       61 cgaatcacag agattcgacg acgcccgcgc cgttctgcag ggtgccgccg aagactaccc
      121 cgagcacctg aacctgttca tgacggccga ggcccggatg ctcatcgagg caggcaggcc
      181 cgaggacgcc cttggcgtca tgaagaccgc cctcgccgag caacccgacg acgtctccct
      241 cgcctacgat acggcgatga tcgcccagaa cgtcggtcac ttcgaagaga ccgagtccat
      301 tctcaaggac atcctctccg acgatcccga ccacgttcag gcgggcaacg cgctcgccta
      361 cctgtgggtg accagagaca tgaaccttcc cgaggcgcgc cgccttcttg aacacgccta
      421 ccgtctgtcg cccgtcgatc cgtacgtgct cgacagcatg ggctggctct gcttcaagga
      481 aggacgctac gaccaggcgg cggagttcac gctcgcctcc ctcaagcgcc tcttcgacgt
      541 cgaggtggcc tgtcacctcg tcgagatcct cgccgtctcg ggccgcgacc acgaggcgga
      601 gaagctcttc cgcgagctcg agcagcgcgc gggctcggac gccctcgtgc ttgacctcgc
      661 caagcgcctc aactacaaga ccaaatgaaa cgtcgaatcc ttctggcgct tcccgcagcg
      721 gccgcagtgc tgacgctcgc aggctgcgtg acgggacagc ccccgctttt tgagagatac
      781 ggccgcttcg cgctcgtctc gacgtccgcc tcgggcgaac gagaggccgt gaacgggcgc
      841 ttcagcctgc ggcgcacgtc cgcaggcctg cgcctcaatc tgatgacgcc cctcaacggc
      901 attctggcaa gcatcgaggt gacgccctcg ggcgccgtgc tcgaaacctc gcagaacgag
      961 tctcccctca gagcggccac cgccgaggag ctcatgctcc gcacgacggg cgtcgccgtt
     1021 cccgtcgaaa ccctcgagcg ctggctcaca atgaagggcg accgcgcctc cgaatacggt
     1081 tggagcgtcc gcgtgctcaa gcgcagggac gacggcatgc ccggcacgat ccgcgcttcc
     1141 tccacaggcg tctcactcac actcaccatg gacgaggact gacatgttgt ccgacagcgc
     1201 cctgctcggc ctgcccgcgc cggccaagct caatctcttt ctgcacgtcg tgggccgccg
     1261 ccccgacggc aagcacctgc tcgagtccgt cttcacgctc atcgacctgc acgacacgat
     1321 cgacctcttc aggcttgagg aacccgtcgt cgagcgcacg ggcgacgtga tcggcaacgt
     1381 cgagaaagac ctctgcgtgc gcgcggcccg cctgctcatg gagaccttcg acgtcaggag
     1441 cggcgtgcgc atccatgttg aaaaacgcat tcccgcggga gcgggcatgg gaggcggcag
     1501 ctccgacgcc gcgaccgtcc tcatcggcct caacaggctc ttcggactcg gcctttcgcg
     1561 cagcgccctg atggagctcg gcgagaggct cggcgccgac gttcccttct tcattttcgg
     1621 ccgaacgggc ttcgtggagg gcattggaga gatcatcacc ccgctggaaa tcccccctgc
     1681 ctcatacgcc gtcatctggc ccggaagggg catttccacg gccgagattt ttgctgcgcc
     1741 aaacttgaca agggatacca aatcactgaa aatagacatc ctttcaggca aggcactgga
     1801 aatctggccg aatctgtacg ggcacaacga cctgcagaat gtggcgcaag agcttgagca
     1861 cagggtgacg gaaggtctcg gaatgctcgc ctgcggcacg cacgacgcca gaatgacagg
     1921 gtccggcagc gccgtcttcg gcgtcatcgc cgatccgtgc agctacaggc tgccgcagct
     1981 gcccgacggc tggaaaggat ttgccgcaaa gtcgctccgg gagcatccgc tctcggcttg
     2041 gcttgactag aaacaatcgg taggggtata gccaagctgg cctaaggcac cggattttga
     2101 ttccggcatt cgaaggttcg aatccttcta cccctgccag cacaaaaacg aaagaggatg
     2161 ggaggcaaca aatgcgctcc ctttcttttt tcagggccgt ctttgtgtta ctctgacggc
     2221 ttccctgttg ttaactgcga gagcgcaagc ccccgcccac gaaaagagct taagtcatgg
     2281 atcccatggt tccggaaagt atgatggtct tcacgggcaa cgccaacccg aaacttgccc
     2341 aggctgtcac gaaatatctc aacattcccc ttggctccgc caccgtcaac cgcttcagcg
     2401 acggcgaagt catggttcag ctgctcgaca atgtgcgcgg caaggacgtc ttcatccttc
     2461 agagcacctg cgctccgacg aacgacaacc tgatggaact catgatcatg gttgacgccc
     2521 tcaagcgtgc gtccgcccgc cgcatcacgg ctgcgatccc ctactacggc tatgcccgtc
     2581 aggaccgccg tccgcgttcc acgcgcgtgg ccatctccgc caaggtcgtc gccaacatgc
     2641 ttcaggccgt cggcgttgac cgcgtcctca cgatggacct ccatgccgac cagatccagg
     2701 gcttcttcga catcccggtc gacaacatct acgccacccc ggttcttctc aacgacctcg
     2761 tcgaacgcaa gtacgacgac ctgatggttg tttccccgga cgtcggcggc gttgtccgcg
     2821 cccgcgccat ggccaaggct ctcgaagtcg acctcgccat catcgacaag cgccgcccgc
     2881 gcgccaacgt cgctgaaatc atgaacatca tcggtgaagt caagggccgc acctgcgtca
     2941 tcaccgacga catcgttgac acggccggca cgctctgcaa cgccgccggt gccctgaagg
     3001 agcgcggcgc caagcgcgtc gtcgcctaca tcacgcaccc ggtgctctcc ggcgccgccg
     3061 ttgaacgcat catgaattcc gctctcgacg agctggtcgt tacggacacg attccgctgt
     3121 ctgccgccgc cgaagcctgc ccgaagat
//
LOCUS       DBMDTI010000237         3123 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6973_length_3123_cov_4.109530, whole genome shotgun sequence.
ACCESSION   DBMDTI010000237 DBMDTI010000000
VERSION     DBMDTI010000237.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6973_length_3123_cov_4.109530"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..98
                     /gene="rrf"
                     /locus_tag="ACZPB6_08980"
     rRNA            <1..98
                     /gene="rrf"
                     /locus_tag="ACZPB6_08980"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            653..1783
                     /gene="lptF"
                     /locus_tag="ACZPB6_08985"
     CDS             653..1783
                     /gene="lptF"
                     /locus_tag="ACZPB6_08985"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HIR1184636.1"
                     /translation="MIFKRSMVSELANSAGGVFTVLFTIVLAVGMVRILSLAAGGRID
                     NGTVLQMVLYNALTNLPPLLTLSLFIAVLMTMMRWWQDNEMVVWFSSGGRSLFSWVPP
                     VLRFALPFVVAVGVLSIVISPWARSQTEITRNEFAQRDEVNRLAPGRFIETMGGKRVF
                     FIESQGESPNEVGRIFMAERTSKGESTVTAERGTVEINSEGDRYIVLHNGRRHETALD
                     TPVTRVVEFDEYAVRLDIKVDKRLESGKVSAQPMTVLFAGMTPDQQAQIFWRFSWPAA
                     AFLLALFAIPLSASNPRAGRSLNIIIAALVFILYLNAISVVQTWIEQGKFGYMTGLFL
                     LHGFVAMLCALFFIRRVYMMRWLPVWCSPWYWRRRLISSKEN"
     gene            1785..2924
                     /gene="lptG"
                     /locus_tag="ACZPB6_08990"
     CDS             1785..2924
                     /gene="lptG"
                     /locus_tag="ACZPB6_08990"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR1184637.1"
                     /translation="MRVITRQLAKEILFSSGFVLIALVALFAFFDLVGQLDDVADGRT
                     IMQAFTLTAFTLPARIYEVMPLAALLASVYTMSRWAATSEFTCLRVAGMSPVTLALSL
                     MLPGIILVGITYTFGEFIAPAASRYAAEVKSISNNQTTRTARGYSSGVWVRDVTTGER
                     GLEIDRYINVKYLNATDSAETGGWRLFEFDKQGRIRVFIESESAHFEPGKGWLLKDVV
                     RYDYPVFDAADNKPLESRIEMTKADELLLHSSLGPDTLSVMTVKPDTMSMRDLDRYVS
                     HLKKNNQQSEHYEVAFWNKAFYPLATLVMIALSMPFAYMNARSGGLAIKMFVGVMIGI
                     VFYALNNLFSYLGVLNTWSPIVVSLVPTVSMLIMAAVAMFLVERR"
BASE COUNT          584 a          911 c          846 g          782 t
ORIGIN      
        1 ggtggtccca ctccttccca ttccgaacag gacagtgaaa caccttatcg ccgatgatag
       61 ttggttgtat ttccagtgaa agtaggacat cgtcaggctc atcattccaa gaaccccggt
      121 ttcgaaagag atcggggttt ttgcttttca gtatattgtc cgcccctgtt ccatctatat
      181 gaaattcaag gagtttccaa taatatccac aatgaagagg gtgtggataa gttatgcaca
      241 cgtgactttt gccagctctc gccgccccca gtttgcaggc gttttcaggc tcctgttttt
      301 aatgtgttga aatatatgga taatttgcgg ttatccacaa aaccgtgacc tctccgaaaa
      361 gttttccaca gttttaaacc ttcagcacat cctctagttt ctcgcttaag ttgttgttgt
      421 caatggaaaa tctgtaaacg cgggagcgtt ttgtcaatag ttgtccacag gctggggcct
      481 aagtgtggaa aactcgtgtt tgaacggctt tgcgcagcct ggctgcttga agcgtattcg
      541 gtcgaacgac ttcaattcgt ctaaaatcaa tattttgacg aagtccgcgt gccgtcccgg
      601 cggcagcgtt ctcctgtcgg cccaacctac atttgcgcgg aatctctgct ccatgatctt
      661 caagcgttcc atggtttctg aactggccaa cagtgccggc ggtgtcttca ccgttctgtt
      721 caccattgtt ctggcagtcg gtatggtccg cattctcagt cttgcggccg gtggtcgcat
      781 cgacaacgga accgtgctgc agatggtgct ctacaacgcg ctgacgaacc tgcctccgct
      841 tctgacgctc tcactcttca tcgccgtcct gatgacgatg atgcgctggt ggcaggacaa
      901 tgaaatggtc gtgtggttct cttccggcgg ccgctccctc ttttcatggg ttcctcccgt
      961 cctgcgcttc gccctgccct ttgtcgtggc cgtgggcgtg ctctccatcg tcatttctcc
     1021 gtgggcccgc tcccagaccg aaatcacccg caatgaattc gctcagcgtg atgaagttaa
     1081 ccgtcttgca cccggccgat tcatcgagac gatgggcggc aagcgtgtct tcttcatcga
     1141 gtcccagggg gagtcgccca acgaagtggg ccgcatcttc atggctgaac gaaccagcaa
     1201 gggcgagtcg acggtgactg ccgagcgcgg tacggtcgaa atcaattcgg aaggcgaccg
     1261 ctacatcgtt cttcacaacg gccgccgtca tgaaacggcg cttgataccc ccgtcactcg
     1321 cgtggtcgag ttcgacgagt acgctgtacg tcttgacatc aaggttgaca agaggctcga
     1381 gtccggcaag gtctcggctc agccgatgac ggtgctcttt gcaggcatga cgcccgacca
     1441 acaggcccag atcttctggc gcttctcctg gcctgcggcc gccttcctgc ttgcgctgtt
     1501 tgcgattcct ctctctgcgt cgaatccccg cgcaggtcgc agcctcaaca tcattattgc
     1561 cgctctggtg ttcattcttt acctcaatgc catttcggtc gtgcagactt ggattgagca
     1621 gggcaagttc ggctacatga ccggtctttt cctgctgcac gggttcgtcg ccatgctctg
     1681 cgcgctcttt ttcattcgtc gcgtctacat gatgagatgg cttcccgtct ggtgcagccc
     1741 ctggtattgg cgtcgtcgcc tgatttcttc gaaggagaac taagatgcgc gtcattaccc
     1801 gccagctggc caaggaaatt ctcttctcgt cgggcttcgt gctgatcgca ctggttgcgc
     1861 tctttgcctt ctttgatctg gtcggccagc tcgacgacgt ggctgacggc cgcacgatca
     1921 tgcaggcgtt cacgcttacg gcctttacac tgcccgcccg catctacgaa gtgatgccgc
     1981 tcgctgcgct gctggcctcc gtctatacga tgtcccgctg ggctgcgaca agcgagttca
     2041 cctgtctgcg cgtcgcaggc atgtcgcccg tgacgctcgc tctttccctg atgcttcccg
     2101 gcatcatcct cgtgggcatt acctacacct tcggcgagtt catcgcgccg gcggcctcgc
     2161 gttatgcggc cgaggtgaag tccatttcga acaatcagac cacccgaacc gcccgcggat
     2221 attcctcggg cgtctgggtg cgcgacgtga cgacgggcga gcgcggtctt gagattgacc
     2281 gttacatcaa cgtgaagtat ctcaatgcga ccgattcggc cgagacggga ggctggcgtc
     2341 tgtttgaatt cgacaagcag ggccgcatcc gtgtcttcat cgaatccgag tcggctcact
     2401 ttgagcccgg caagggctgg ctcctgaagg acgtcgtacg ttatgactat cccgtcttcg
     2461 atgctgcgga caacaagccg cttgaatccc gcattgaaat gacgaaggcg gacgagcttc
     2521 ttcttcactc ctccctgggt cccgacactc tcagtgtcat gaccgtgaag cccgatacga
     2581 tgtccatgcg agacctcgac cgctacgtga gccatctgaa gaagaacaac cagcagtccg
     2641 agcattacga ggttgcgttc tggaacaagg ccttttatcc gctcgccacg ctcgtgatga
     2701 tcgcgctgtc gatgccgttc gcctacatga atgcacgctc cggcggtctg gcgatcaaga
     2761 tgttcgtcgg cgtcatgatc ggcattgtct tttacgcgct caacaacctc ttctcgtatc
     2821 tgggcgtact caacacctgg tcgccgattg tggtgagtct ggtgccgaca gtgagcatgt
     2881 tgatcatggc ggccgtcgcc atgttcctag tcgagcggcg atagcttcag gtccagcatt
     2941 cgaaaagcga aaggcccgca gttcaacctg cgggcctttc gcttgtgtgc tcggcatggg
     3001 cacgttctaa cgggtgaaag tcccgagagg ggccggcagt ggcaacctca tagccaaaag
     3061 caaggtgtgc gtcgcgaggc gtaggctgaa agaagctgga ggcaaaccct tgagctgatg
     3121 aac
//
LOCUS       DBMDTI010000238         3123 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_6974_length_3123_cov_2.383190, whole genome shotgun sequence.
ACCESSION   DBMDTI010000238 DBMDTI010000000
VERSION     DBMDTI010000238.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6974_length_3123_cov_2.383190"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            102..1718
                     /locus_tag="ACZPB6_08995"
     CDS             102..1718
                     /locus_tag="ACZPB6_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HIR1184638.1"
                     /translation="MHKQLTPVAAALALLGLLAGTPAQAADNQCAACHANVAKDHAGA
                     AHKDLACTTCHTGTEAHLKDMKKRPAVNMDPAQCGACHQQQYKSAFMTSDRPARQSKK
                     AAGGPAPDPFFDRALGGHGFTKEHDLPRAHTFMAIDQFIVDRAFGGRFEPKDGWLYTT
                     LEGGKSYKAWDVLKDNYPDNNEQKAHKPGTAAAANGVCWSCKSTDLMLDWAYMGDKAE
                     GAKFHRGSNPVDVVRNVQHGMNCNFCHDPHSAKPRIMRDALIDAMTREGKMNVYKDFA
                     ARQAKLEVKDVGVRGFDRKVAMLDRADSRLMCAQCHVEYVCNPGMNVKTGEKVGFDSR
                     LTNLFPWVNADQIEAYYDEVGFRDFKHNLTGATLVKMQHPDAETYFGSKHDKVGADCA
                     SCHMPKVKDENGKTYTMHWATSPKHYVKETCLSCHKDKNEKQMVAAIDAMKGHFDGKV
                     REAESRMNDMFNAFELAKTVGVSEEVLAKARKLHESAHINWEYWTAANGAYFHNHDMA
                     VRSLAKSAKAASDATALLRKAIDEKVATKK"
     gene            1868..2773
                     /gene="ccmI"
                     /locus_tag="ACZPB6_09000"
     CDS             1868..2773
                     /gene="ccmI"
                     /locus_tag="ACZPB6_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HIR1184639.1"
                     /translation="MSDEILLYLILLLMLVLAVGALVPSLLRVGREEASVEREDKAYG
                     SAVTEALRRQRAQLDEDLKSGAVSAEQYEILADDLRRRALEEHDAVFHDPNDGAGRRA
                     GSFSGPVVTAAVVAGVTAVSVGLYAVNGAPELIGLEKAQAVYDGRADTPSIERYLESS
                     PKDGRAWVLLARRHVDEENFPKAAEAYRRGCAANAKVKADPAVMLELAATLLTIGGPD
                     SMREAQPLAQGAVDGRPGDMKAVEILAVAASANEDWRTAAESLSLLMSTMNPDTPEYV
                     QYEMTLKRLRELAASQKTPLPSTSR"
     gene            2909..>3123
                     /locus_tag="ACZPB6_09005"
     CDS             2909..>3123
                     /locus_tag="ACZPB6_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184640.1"
                     /translation="MNIRKAFLHKAVPAACVLFAAGTIFAADRADLMGYVPGAEVMKV
                     DVTDGRIDTISGIIYTFCPGRLLRYGPA"
BASE COUNT          630 a         1084 c          932 g          477 t
ORIGIN      
        1 acctcggaag ggtttaagaa aggcttaagc aaacttaaaa ttttgaaaaa gacgaggacc
       61 tcgccagacc ccgcgcgacg gccttgtcac aggagaacaa catgcataaa cagctcaccc
      121 ccgtcgccgc cgcgcttgcg cttcttggtc ttctcgcggg cactcccgcc caggcggccg
      181 acaatcagtg cgcagcctgc cacgccaacg tcgccaagga tcatgcgggc gccgcccaca
      241 aggatctcgc ctgcacgacg tgtcacaccg gcaccgaagc gcatctcaag gatatgaaga
      301 agcgtccggc cgtcaacatg gacccggccc agtgcggcgc ctgccaccag cagcagtaca
      361 agtccgcctt catgacgtcc gaccgtcccg cccgccagtc gaagaaggcg gccggcggtc
      421 ctgcacccga cccgttcttt gaccgcgcgc tcggcggcca cggcttcacg aaggagcacg
      481 accttccgcg cgcccacacg ttcatggcga tcgaccagtt catcgtcgac cgtgcgttcg
      541 gtggccgctt cgagcccaag gacggctggc tctacacgac gcttgagggc ggcaagtcct
      601 acaaggcctg ggacgtcctc aaggacaact atcccgacaa caacgagcag aaggcccaca
      661 agccgggcac ggccgctgcc gccaacggcg tgtgctggtc ctgcaagtcg accgacctga
      721 tgctcgactg ggcctacatg ggcgacaagg ccgagggcgc gaagttccac cgcggctcca
      781 accccgttga cgtcgttcgc aacgtccagc acggcatgaa ctgcaacttc tgccacgacc
      841 cgcacagcgc caagccgcgc atcatgcgcg acgccctgat cgacgcgatg acgcgcgaag
      901 gcaagatgaa cgtctacaag gacttcgcgg cccgccaggc caagcttgag gtcaaggacg
      961 tcggcgtccg cggcttcgac cgcaaggtcg ccatgctcga ccgcgccgac tcccgcctga
     1021 tgtgcgccca gtgccacgtc gaatacgtct gcaaccccgg catgaacgtc aagacgggcg
     1081 agaaggtcgg cttcgacagc cgtctcacga acctcttccc atgggtcaac gccgaccaga
     1141 tcgaggccta ctacgatgaa gtcggcttcc gcgacttcaa gcacaacctc accggcgcga
     1201 cgctcgtcaa gatgcagcat cccgacgccg aaacgtactt cggctcgaag cacgacaagg
     1261 tcggcgccga ctgcgcttcc tgccacatgc ccaaggtgaa ggacgaaaac ggcaagacct
     1321 acacgatgca ctgggccacc tcgcccaagc actacgtgaa ggagacctgc ctctcctgcc
     1381 acaaggacaa gaacgagaag cagatggtcg ccgcgatcga cgcgatgaag ggccacttcg
     1441 acggcaaggt ccgcgaagcc gaatcccgca tgaacgacat gttcaacgcg tttgaactcg
     1501 ccaagaccgt cggcgtgtcc gaagaggtcc tcgccaaggc ccgcaagctc cacgaatccg
     1561 ctcacatcaa ctgggaatac tggactgccg ccaacggtgc gtacttccac aaccacgaca
     1621 tggccgtgcg cagcctcgcc aagtctgcca aggcagcctc cgacgccacc gcgctgctga
     1681 ggaaggccat tgacgaaaag gtcgctacca agaagtaatc cgcctcgggc tctcttcggg
     1741 gcccggcgat gatccgacga aacgggcgtc tcctcgcgga gccgcccgtt tcatatatag
     1801 tgatgggaca aacacgacct gcgctccgag cgcaggcaga cgaacaacgc ctccggaacg
     1861 aacgcacatg tccgacgaaa tccttctcta tctcatcctc cttctgatgc tcgtcctggc
     1921 cgtcggagcg ctcgtgccgt cgctccttcg cgtgggcagg gaggaggcga gcgtcgagcg
     1981 cgaggacaag gcctacggct cggccgtgac cgaggcgctg aggcgacagc gcgcccagct
     2041 cgacgaggac ttgaagtcgg gcgccgtgag tgcggagcag tacgagattc tcgccgacga
     2101 tcttcgccgc cgcgcgctcg aggaacacga cgcggtcttc cacgatccca acgacggcgc
     2161 aggccgccgt gcgggcagct tctcgggccc cgtcgtgacg gccgccgtgg tggcgggcgt
     2221 cactgcggtt tcggtcggcc tctatgccgt gaacggcgcg cccgagctca tcgggcttga
     2281 gaaggcccag gcggtctacg acggacgcgc cgatacgccc tcgatcgagc gctacctcga
     2341 gtcgagcccg aaggacggcc gcgcctgggt gctgcttgcg cgccgtcatg tcgacgagga
     2401 gaacttcccg aaggcggccg aggcctaccg caggggatgc gccgcgaacg ccaaggtgaa
     2461 ggccgatccg gccgtgatgc tcgaactcgc cgccacgctt ctcacgatcg gcggtcccga
     2521 cagcatgcgc gaggcccagc ctctggcgca gggcgccgtc gacggacgtc ccggcgacat
     2581 gaaggccgtc gagatcctgg ccgttgcggc ctccgccaac gaggactggc ggacggcggc
     2641 cgaaagcctc tcgcttctca tgagcaccat gaatcccgac acccccgagt acgtccagta
     2701 cgagatgacg ctcaagcgcc tgcgcgagct cgcggcctcg caaaagacgc cgcttccttc
     2761 gacttcccgc tgagctccac ctgaattccg actgaagtcc gactgagagc gggcggttgg
     2821 acgagggtag aattttctgg tcggagtccg ttctccggca atgcagcttc tcaagccccc
     2881 ctcgtaaccg acaagaagga atttttcaat gaacatccgc aaggcctttc tgcacaaggc
     2941 cgttcccgcc gcctgcgtgc tcttcgcggc cggaacgatt ttcgccgccg accgcgccga
     3001 tctgatgggc tacgtgcccg gcgccgaggt catgaaggtc gacgtgaccg acggccgcat
     3061 cgacacgata tcgggcatca tctatacctt ctgtccgggc aggctgctcc gctacggccc
     3121 agc
//
LOCUS       DBMDTI010000239         3114 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7001_length_3114_cov_2.294059, whole genome shotgun sequence.
ACCESSION   DBMDTI010000239 DBMDTI010000000
VERSION     DBMDTI010000239.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7001_length_3114_cov_2.294059"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="ACZPB6_09010"
     CDS             complement(<1..974)
                     /locus_tag="ACZPB6_09010"
                     /inference="COORDINATES: protein motif:HMM:NF019335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR1184641.1"
                     /translation="MVLHRTLIAAAVLAATAPAWASTEEAARAVEEPAQADAAEPASD
                     EGESVVVRDRAGESLTSFTHLTREDIARRPTEDGNITDLLKSNPAVQFSNHGDGSLQM
                     GEIKPSNISIHGSVAYQNNYTLDGISTNSDLDPAEQTSVTTTRLGGSDEQGFYVDSRL
                     IDSVTVYDHNIPASFGGFTGGVIDAQTRSWSGENHISVFGRMTKSGWSRIHTDSALDV
                     GSGDADVSKPAQFQTDFEKKTYGFTGEWGITDNLGVVVGYTRRESKIPTIQVPGTRVS
                     IVPDDQNWTGWGVLETPVAGGTQTQRRTADNFFAKATLYATPWTEASLA"
     gene            complement(1412..3070)
                     /gene="ilvD"
                     /locus_tag="ACZPB6_09015"
     CDS             complement(1412..3070)
                     /gene="ilvD"
                     /locus_tag="ACZPB6_09015"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR1184642.1"
                     /translation="MRSDPLKKGINAAPQRALLKALGLCDAEIERPLIGVVSSQNDIV
                     PGHVFLDKITDAVKQGVALGGGVPIVFPAIAVCDGLAMGHKGMKYSLVSRELIADSVE
                     AMATAHPFDGLVMVAACDKNVPGLLMAAARLNIPSIVISGGAMLAGRACGRKLSYSDI
                     SEGVSRFRTGQIDAKTFLELENKSCPTCGSCSGMFTANSMNCLTEVLGMALPGNGTIP
                     AVMSARYRLAKEAGLAVMELVRNNVCPRDIMTKEAFENALTVDMALGCSTNSMLHLPA
                     IAHECGFELDLTEANAISARTPNLCHLAPAGHRYIEELDEAGGIPAVMNELARAGLLH
                     TNLPTVTGKTVGENIEGREILDAEVIRPVSDPFSKEGGIAVLKGNVAPDGCVVKRSAV
                     APEMLMHTGPARVFDCEEDALEAIYGGCINPGDVVVIRYEGPKGGPGMREMLNPTSAI
                     MGSGLGGCVALITDGRFSGATRGAAIGHVSPEAAVGGPIGLLEEGDMLTIDIPANTIS
                     VDLTNEELARRRAAWKPRAPRITTGYLARYAKLVTSGMSGAVLE"
BASE COUNT          515 a          942 c         1088 g          569 t
ORIGIN      
        1 gcgagactcg cctccgtcca gggcgtcgcg tagagcgtgg ccttcgcgaa gaagttgtcg
       61 gccgtgcggc gctgcgtctg cgtgccgccc gcgaccggcg tctcaagaac gccccagccg
      121 gtccagttct ggtcgtcggg cacgatggag actcgggtgc ccggcacctg aatggtcggg
      181 atcttcgact cgcggcgggt gtagcccacg acgacgccga gattgtcggt aatgccccat
      241 tcgcccgtga agccgtaggt cttcttttcg aagtcggtct ggaactgggc gggcttcgag
      301 acgtccgcgt cgcccgagcc gacgtcgagc gcggaatcgg tgtggatgcg gctccagccc
      361 gacttcgtca tgcggccgaa cacgctgatg tggttttcgc ccgaccagct acgggtctga
      421 gcgtcgatga cgccgcccgt gaagccgccg aaggaggcgg gaatgttgtg gtcgtagacg
      481 gtaacgctgt cgatgaggcg gctgtcgacg tagaagccct gctcgtccga gccgccgagg
      541 cgcgtcgtcg tcaccgaagt ctgctcggcg ggatcgaggt cgctgttggt cgagatgccg
      601 tcgagcgtgt agttgttctg ataggcgacc gagccgtgga tcgaaatgtt cgacggcttg
      661 atctcgccca tctgcagaga gccgtcgccg tgattggaga actggaccgc aggattgctc
      721 ttgaggaggt ccgtgatgtt gccgtcctcc gtcggacgcc tggcgatgtc ctcgcgggtg
      781 agatgcgtga acgacgtgag gctttcgccc gcacggtcgc gcacgacgac ggattcgcct
      841 tcgtctgagg ctggttcggc ggcatccgcc tgcgcaggct cctcgacggc gcgcgcagcc
      901 tcttcggtcg aggcccaggc gggagccgtc gcggcaagca cggcggcggc aatgagagtt
      961 cggtgaagca ccatgatccg gtagtccgat tgaaaaaagt ccagagatgt gcccgtgcgc
     1021 ccacgggcgc gcagttcccc gaatcctata tgagaatggc tctcattctt tcattattct
     1081 ttctatctgc ggatgcttct catttgcatt cgcctatggt ggacgacgat catcacatag
     1141 ttgagaatga ctctcgccta ctcgcagtgc gaaagagcgt gggcaagtgc cgtcacccgt
     1201 cctcggacac gttcggggtc ggacgcgcgg accgataccg caaacacgtg atgtctttac
     1261 gcaaatccca agggtttacc cgcggctcat tcaggcagga atccgcggcc gtccgggatc
     1321 cagagaacga cgaagcccct cgtccgatgc aggacaaggg gctttccgga atttcattca
     1381 tgcacgcccg aaggcgcgcg gactgcgtcc gtcattcgag cacggcgccg ctcatgcccg
     1441 aggtcacgag cttggcgtag cgggcgaggt agcccgtcgt gatgcgcggg gcgcggggct
     1501 tccaggcggc cctgcgtcgg gcgagctcct cgttggtgag gtcgacgctg atggtgttcg
     1561 cggggatgtc gatcgtgagc atgtcgcctt cctcgaggag tccgatcgga ccgcccacgg
     1621 cggcctcggg cgagacgtgg ccaatggcgg cgccgcgggt tgcacccgaa aagcggccgt
     1681 cggtgatgag cgcgacgcat ccgccgaggc ccgagcccat gatggcggag gtcgggttga
     1741 gcatctcgcg catgccggga ccgcccttgg gaccttcgta gcggatcacg acgacgtcgc
     1801 cgggattgat gcagccgccg tagatggcct cgagcgcatc ctcctcgcag tcgaagacgc
     1861 gggccgggcc cgtgtgcatg agcatttcgg gcgccacggc cgagcgcttg acgacgcagc
     1921 cgtcgggcgc cacgttgccc ttgaggacgg cgatgccgcc ttccttcgag aacggatcgg
     1981 aaacgggacg gatgacctcg gcgtcgagga tctcgcggcc ctcgatgttc tcgcccacgg
     2041 ttttgcccgt aacggtcggc aggttcgtgt ggagcaggcc cgcgcgggcg agctcgttca
     2101 tgacggcggg gatgccgccc gcctcgtcga gctcctcgat gtagcggtgg cccgcaggag
     2161 cgaggtggca gaggttgggc gtgcgcgcac tgatggcgtt ggcctccgtg agatcgagtt
     2221 cgaacccgca ctcgtgcgcg atggcgggca ggtgcagcat cgagttcgtg gagcagccga
     2281 gcgccatgtc gacggtgagc gcgttctcga acgcctcctt cgtcatgatg tcgcgcggac
     2341 agacgttgtt cctcacgagc tccatcaccg caaggccggc ctccttggcg aggcggtagc
     2401 gcgcggacat gacggcggga atcgtgccgt tgccgggcag cgccatgccg agcacctcgg
     2461 tgaggcagtt catggagttg gccgtgaaca tgcccgagca ggagccgcag gtcggacagg
     2521 acttgttctc aagctcgagg aaggtcttcg catcgatctg gcccgtgcga aagcggctca
     2581 cgccctcgga gatgtcggag taggagagtt tgcggccgca ggcgcggccc gcgagcatcg
     2641 cgccgcctga gatcacgatg ctcgggatgt tgaggcgggc ggcggccatg agcaggccgg
     2701 gcacgttctt gtcgcaggcg gccaccatca cgaggccgtc gaacggatgc gccgtggcca
     2761 tcgcctcgac gctgtcggcg atgagctcgc gcgaaacgag cgaatacttc atgcccttgt
     2821 ggcccatcgc cagaccgtcg cagacggcga tggccgggaa gacgatgggc acgccgccgc
     2881 ccagggcgac gccctgcttc accgcgtccg taatcttgtc aagaaagacg tgtcccggaa
     2941 cgatgtcgtt ctgcgaggag acgacgccga tgagcgggcg ctcaatctcc gcatcgcaga
     3001 gaccgagagc cttgagaagc gcgcgctggg gagccgcgtt gatgcctttc ttgaggggat
     3061 cagaacgcat gtagaagttt cctgtggtga attgatgaag agagcctttc aagt
//
LOCUS       DBMDTI010000240         3109 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7019_length_3109_cov_2.379654, whole genome shotgun sequence.
ACCESSION   DBMDTI010000240 DBMDTI010000000
VERSION     DBMDTI010000240.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7019_length_3109_cov_2.379654"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="ACZPB6_09020"
     CDS             complement(<1..493)
                     /locus_tag="ACZPB6_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184643.1"
                     /translation="MMKLIPSPPLKSVAALAAVAVFTCMLAASPVRAGAFEEYRAADM
                     KLQSVREGDAEGLRAAGVLVESFSKRWSKGAASERTMAAYLMLRWGIASGRHQAAYGA
                     VFVLLELKPSPEQRPGLLKLAAQLGYQSGRFEAVPGHVDAWIEVKGMPRTATERAETA
                     EMMT"
     gene            complement(490..1161)
                     /locus_tag="ACZPB6_09025"
     CDS             complement(490..1161)
                     /locus_tag="ACZPB6_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR1184644.1"
                     /translation="MQPTQGIPDRLAAALAALVVTGAVLTLILMLTMGGRHEAKRAVT
                     LPVVYWSGPPPEAPDETPPEEVPVTAETVPEVSAPPKPVTFAFESPAIAAAGLPMPKY
                     EIGRPTVDRVMTVPDFSFESPAAPAAAAPSAPAVSSIALARPVHRVPPQYPVKAKQQG
                     IEGYVVMDLRINAEGRVEDVRVVREKPEGVFRASALRVLRRWAFMPPAEPAWQRLTIR
                     YELEK"
     gene            complement(1136..1543)
                     /locus_tag="ACZPB6_09030"
     CDS             complement(1136..1543)
                     /locus_tag="ACZPB6_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR1184645.1"
                     /translation="MRFEHRDGRERDAGIDMTPMMDIVFIMLIFFVLTASFTQTQTLD
                     VERPSTASHDRSAPEALVVSVDRSNIIWFDGRQVGKEEVTGLVRGAAAGRPVSVVVNA
                     DRLVPSGLLIEVIDQIRLGGVANVAVATNAGHS"
     gene            complement(1579..2136)
                     /locus_tag="ACZPB6_09035"
     CDS             complement(1579..2136)
                     /locus_tag="ACZPB6_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR1184646.1"
                     /translation="MNEALAGFWWFEDLSLLMDRGGHVLWAILVLSTVLWMILLTGAR
                     RLKDAEKAWTAATAAPALEWATATAASRRRMMQAALVRVRRTAAAEAAWARVIVALLP
                     LLGLLGTVTGMIESFETLSAAGGSDPGGLTGGISAALLTTLAGLVTALPGVFAVHRLE
                     RRTDRLADKVRARLRRFMRPSCCRS"
     gene            complement(2151..>3109)
                     /locus_tag="ACZPB6_09040"
     CDS             complement(2151..>3109)
                     /locus_tag="ACZPB6_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR1184647.1"
                     /translation="LLTESVMKTQIGRSKAFMDDAPVSKTDGVARLRRHWVRALGAVA
                     GSAGVRTVEDAVIYDAAGAPSRGTVTLYGPFAAKAGDGAWLRYLPSERAWRRLADDPG
                     IPAGVVALDPSFGRLLAGYAERHSAWAWMKPAGVIGIFIGLVAAAALLLGFLRWTALA
                     LEERRVRRQLEDMENPREDNSLGRMLAAAKESTDELLEAKLDAAIVAERPGFERGVAT
                     LAVLAGIPTLLGLLGTVSGMIETFTVMSEHGSAEPGLLAGGIAEALVTTELGLVSAIP
                     ILLLHCAVKTKRDALFSHLEEAASTLAVREAAGCCGACRRDE"
BASE COUNT          518 a         1094 c         1042 g          455 t
ORIGIN      
        1 gcgtcatcat ctccgccgtc tcggcgcgtt cggtcgccgt gcgcggcatg cccttcactt
       61 caatccaggc gtccacgtgt ccgggcacgg cctcaaaccg cccgctctga taacccagct
      121 gggcggcgag cttcagaagt cccggcctct gctcgggcga aggcttgagc tcaagaagca
      181 cgaacaccgc accgtaggcc gcctgatggc gacccgacgc gatgccccag cgaagcatca
      241 ggtaggcggc catcgtgcgc tcggaggcgg cgcccttcga ccagcgcttc gagaaggact
      301 cgaccagcac gcccgctgcc cgaagcccct cggcgtcgcc ttccctgacg gactgcagct
      361 tcatgtcggc cgcacggtac tcctcaaagg cgcccgccct gacgggcgag gccgcgagca
      421 tgcaggtaaa aaccgcaacg gccgcaaggg cagcgacgga cttcaggggc ggactcggaa
      481 tcagcttcat catttctcca gctcatagcg gatggtgagg cgctgccacg cgggttccgc
      541 gggcggcatg aaggcccagc ggcgcaagac gcgcagggcg gaggcgcgga agacgccctc
      601 tggcttttcg cgcacgacgc gcacatcctc gacgcggccc tcggcattga tgcgcaggtc
      661 catgacgacg tagccctcga tgccctgctg cttcgccttc acgggatact gaggcggcac
      721 cctgtgcacg ggccttgcga gcgcgatcga gctcacggcg ggcgctgagg gcgctgcggc
      781 ggcgggagcg gcgggcgact cgaacgaaaa gtcgggcacg gtcatgacgc gatcgaccgt
      841 gggcctgccg atctcgtact tcggcatcgg caggccggcc gcggctattg cgggcgactc
      901 gaaggcgaac gtgacgggct tcgggggcgc cgacacctcg ggcacggtct cagccgtgac
      961 ggggacctcc tcgggcggcg tctcgtcggg cgcctcgggc ggaggacccg accagtagac
     1021 gacgggaagc gtcaccgcgc gcttcgcctc atgccgtccg cccatcgtga gcatcaggat
     1081 gagcgtgagc accgcgcccg tcacgacgag ggccgccagg gcggcggcga ggcggtcagg
     1141 aatgccctgc gttggttgca acggccacat tggcgacgcc tcccagacgg atttggtcga
     1201 tgacttcgat gagcaggccc gagggcacga gcctgtcggc gttgacgacg acgctcacgg
     1261 gcctgcccgc ggccgcgccg cgcacgagtc ccgtaacctc ctccttgccc acctgcctgc
     1321 cgtcgaacca gatgatgttc gagcggtcca cgctcacgac gagggcctcg ggcgccgacc
     1381 tgtcgtgcga ggccgtcgac gggcgctcga cgtcgagcgt ctgcgtctgc gtgaagctcg
     1441 cagtgagcac gaagaagatc agcatgatga agacgatgtc catcatcggt gtcatgtcga
     1501 tgcccgcgtc acgctcgcga ccgtccctgt gttcaaatct catgatcaaa cctcatttca
     1561 tttttcctga acctgcattc aggacctgca gcacgagggc cgcatgaacc tgcgcagcct
     1621 tgcgcgcacc ttgtccgcaa ggcggtcggt ccttcgctcg aggcggtgca cggcgaagac
     1681 tccgggaagc gccgtcacga gtcccgcaag ggtcgtgagg agcgccgcgc tgatgccgcc
     1741 cgtgaggccg ccggggtcgg agccccctgc ggcggagagc gtctcgaagc tctcgatcat
     1801 cccggtcacg gtgccgagaa gtccgaggag cggcaggagc gccacgatca ctctggccca
     1861 ggcggcctcg gccgcggccg tgcgcctcac gcgcacgaga gccgcctgca tcatgcggcg
     1921 gcgcgacgcg gcggtcgcgg tcgcccactc gagggcgggc gcggcggtgg cggccgtcca
     1981 ggccttctcc gcgtccttca ggcgcctcgc gcccgtgaga agaatcatcc agagcaccgt
     2041 cgagaggacg aggatcgccc agaggacgtg cccgcccctg tccatcagga gcgagaggtc
     2101 ctcgaaccac cagaagcctg caagtgcttc gttcatggcg cctccctgac tcactcgtcc
     2161 ctgcggcagg cgccgcagca gcccgcggcc tcgcgcacgg caagggtcga ggcggcttcc
     2221 tcgagatgcg agaagagcgc gtcgcgcttc gtcttcacgg cgcagtgaag aagcaggatc
     2281 ggaatcgcgg acacaaggcc gagctccgtc gtgacgagcg cctcggcgat gccgcccgcc
     2341 agaaggccgg gctcggccga gccgtgctcg ctcatcaccg tgaaggtctc gatcatgccg
     2401 ctcacagtcc cgagcagacc gagaagcgtc ggaatgccgg cgagcaccgc aagcgtcgcg
     2461 acgccgcgct cgaacccggg acgctcggcg acgatcgccg catcgagctt cgcctcgagg
     2521 agttcgtccg tgctctcctt cgcggccgcg agcatgcggc cgagcgaatt gtcctcgcgc
     2581 ggattctcca tgtcctcgag ctgtctgcgc acacgccttt cctcaagggc gagcgccgtc
     2641 cagcgcagga agcccagaag aagcgccgca gcagccacga gtccgatgaa gatgccgatg
     2701 acgcctgcgg gcttcatcca agcccaggcc gaatgacgct cggcgtagcc cgcaagcagc
     2761 ctgccgaagg acggatcaag cgccacaacg cccgctggaa tgccgggatc gtccgcaagg
     2821 cgacgccagg cgcgctctga aggcagatat cggagccacg cgccgtcccc cgccttcgcg
     2881 gcaaagggac cgtagagcgt caccgttccc ctcgagggcg cgcccgcagc gtcgtagatc
     2941 acggcgtcct cgaccgtcct cacgcccgcc gacccggcga cggccccgag ggccctcacc
     3001 cagtggcggc gcaggcgtgc gacgccgtcc gtcttcgaga cgggcgcatc gtccatgaag
     3061 gccttgctcc tgccgatctg ggtcttcatc accgattcgg tgaggagtc
//
LOCUS       DBMDTI010000241         3086 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7085_length_3086_cov_2.251256, whole genome shotgun sequence.
ACCESSION   DBMDTI010000241 DBMDTI010000000
VERSION     DBMDTI010000241.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7085_length_3086_cov_2.251256"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="ACZPB6_09045"
     CDS             <1..629
                     /locus_tag="ACZPB6_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1184648.1"
                     /translation="YRSIPMTDFSEMTGIPLDQLMYPPRKPSDAFVAALAQGFDVGED
                     AIREHATSLVGTKDRTPIVLSELQKRGLCFRDWIAMTNCSVENAYRLLVGRFPLDPDL
                     KITAALALRIDPEEFIFRYDTSKSTTASEAKRAKRFCELVIAMMGHKKLTKSEFIHKS
                     GVHSFTADTMLAGETTSLLYRTLEKTSAALEIPIETIIDHISGLKPAI"
     gene            complement(766..1254)
                     /locus_tag="ACZPB6_09050"
     CDS             complement(766..1254)
                     /locus_tag="ACZPB6_09050"
                     /inference="COORDINATES: protein motif:HMM:NF019984.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HIR1184649.1"
                     /translation="MRGNCDKSDVFRARRAKFLGYTFAEKTGRTLVHPKSFKRLKDKL
                     RAVFCRARGGSLLRTNGELNAILCGWRQYFRLDNRKGVFEVLDIHIRRHLRKLVWIAW
                     KRPRTRERELHRRGLDGFRAWESANNGRGDWWNANARHMRDAFPLSFFKRHGLYSLLA
                     MR"
     gene            complement(1622..2080)
                     /locus_tag="ACZPB6_09055"
     CDS             complement(1622..2080)
                     /locus_tag="ACZPB6_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184650.1"
                     /translation="MTLRKPIVDEQWLSLNRTIHQVAPPCRNPEHLENAARVLQAEGG
                     LEALARLITTEQTAFLVLAMLEKCGAFQRTAVINGGPCVRISAGVSADAPRKAGVELT
                     RQQMMKIRFSISISPEMRSMCYEGKIPYPDGTIFEKGSTGYFHTKGCRRT"
     gene            complement(2499..>3086)
                     /locus_tag="ACZPB6_09060"
     CDS             complement(2499..>3086)
                     /locus_tag="ACZPB6_09060"
                     /inference="COORDINATES: protein motif:HMM:NF015692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR1184651.1"
                     /translation="RHRSGTPADICGGGDILDNGAGALMRMLPYALLNSPDRLEIVDA
                     AGSLTHSHQISIVICRLYSCYVDELLQGHKPAEALEALIENHFVEIPDYYLPKLQLMP
                     HLSRVSVPNSGFVSDVFMASLWCLINSDNYVDAVLKAVNLGGDADTIGAVTGGLAGII
                     YGQDGDNGIPVCWSHALSRIDYIEELCTACDGMMR"
BASE COUNT          646 a          895 c          884 g          661 t
ORIGIN      
        1 cctacaggtc cattccgatg accgatttct cggagatgac gggcattcct cttgatcagc
       61 tcatgtatcc cccgagaaag ccctccgacg cctttgtcgc agcccttgca cagggttttg
      121 acgtcggcga agacgccatc cgtgaacatg ccaccagtct cgtgggcacg aaggacagga
      181 cgcccatcgt gctctccgag ctccagaagc ggggactttg cttcagagac tggatcgcca
      241 tgaccaactg ctccgtcgag aacgcctaca ggctccttgt cggcagattt ccgctcgatc
      301 ccgacctcaa gatcacggct gctctggccc tgcgcatcga tcccgaagaa ttcatcttca
      361 gatacgacac ctcgaagagc accactgcct ccgaagccaa gcgtgcgaag cgcttctgcg
      421 agcttgtcat tgccatgatg gggcacaaga agctcacgaa gtccgagttc attcacaaga
      481 gcggcgtgca ctccttcacg gccgacacca tgctggccgg cgagacgacc tcgctcctct
      541 acagaacgct ggaaaagacc tcggcggccc tggagatccc catcgaaacg atcatcgatc
      601 atatttcggg actcaagccc gccatttgac gtctgatcgc gtcaaaatcg ggtagagagt
      661 gtccttgcgg acactccctc ccacaccgca gtgctcaatc agtactgagc gttcggacat
      721 gccgttcggc atccggcggt ttctgagtta gttgtagagg gggactcagc gcatcgcgag
      781 aaggctgtag agtccatgtc gtttgaagaa agacagcggg aacgcgtcgc gcatgtggcg
      841 cgcattggcg ttccaccaat ctccccgacc gttgttggcg gactcccatg cgcgaaagcc
      901 atccaggccg cgacgatgga gttcgcgttc gcgtgttctc ggtcgtttcc atgcaatcca
      961 caccaactta cgaagatggc gtcgaatgtg tatgtcaagc acctcgaata cgcctttgcg
     1021 gttgtccaac ctgaagtact gccgccatcc gcacaggatc gcattgagtt ccccattcgt
     1081 tcgaaggagc gacccgccgc gcgctctgca gaagacggct cgaagcttat ccttcagcct
     1141 cttgaagctt ttcgggtgga ctagcgtccg tcccgtcttc tccgcgaaag tgtagccaag
     1201 aaatttcgct cgtcgagcgc gaaagacgtc gctcttgtcg cagttgcccc gcaacttgag
     1261 cgtctcctca atgaacttga ccgagataca cgcctcacat ccgattcctg ttcgtcaggc
     1321 tggtcctttg ccatcggctt cctccatcga agtcgttgcc tttcgcgact tgccgagttg
     1381 ccggtccctc ccctgttcgg gcggacagcg gactttcgcc gcatagacac acgtgcgttg
     1441 ctgggcgctc atacaaaatg cccgctctcc tttcggacag cgggcattct gttttcgcct
     1501 atgcaaaatc ataccggggc cctagtcccg gacgatacga accagagcaa gtcaccccca
     1561 gtacgttctg ttgtcatcct tcagcgtgat gaccgggtcg cgggagacga atccttcagg
     1621 gctaggtccg gcggcagccc ttggtgtgga aatatccggt ggaacccttt tcaaagatcg
     1681 tgccgtccgg atacggtatt tttccctcat agcacatgct gcgcatttca ggcgaaatcg
     1741 aaatcgagaa gcgaattttc atcatctgtt gacgcgtgag ctcgacaccc gccttcctgg
     1801 gtgcgtcggc cgacaccccg gccgagatcc tgacgcacgg accgccgtta atgacggccg
     1861 tacgctgaaa ggctccgcac ttctccagca tggccagaac aaggaacgcc gtctgttcgg
     1921 tggtgatcag gcgcgccagc gcctcgagac cgccctcggc ctgaaggacg cgggcggcgt
     1981 tttcaaggtg ttcgggattc ctgcagggtg gcgcaacctg atggatggtt cggttgagtg
     2041 aaagccattg ctcgtcgacg ataggtttcc tgagggtcat gttttttatg tatggtgtca
     2101 tttctgtagc ttgtgttatt gcgaagaaat gtcgaaacgc ctgagcggtt gcgacgcgaa
     2161 catcgtgcca tgcagatcaa aagttttcaa cccgagcaaa tgacggaaat gtcgtctcaa
     2221 ggcgcatcca aggcggtttt cgccttttga aatcaattta tcagggtgaa gatctctgcc
     2281 gcttcaatag aacgtgatcg ccgtcaacgc ctgaaagaca gaggccttaa ggcgatgttc
     2341 acttccggat ttcatcagga gcatcgacga gacttcgatt tcttgattca aatcaagcct
     2401 tcctgaaatc accctcgaga tcaaggatca agctaaaaaa atggaccgcc tgcaggcagt
     2461 cctcttgagt gtcgaatgga gcgcaaaatg cacggtcgtc agcgcatcat gccatcgcag
     2521 gccgtgcaga gctcttcaat gtagtcgatg cgggaaagcg catgagacca gcagacggga
     2581 atgccgttgt cgccgtcttg gccgtagatg atgccggcaa ggccgccggt aaccgcaccg
     2641 atcgtgtcgg cgtcaccgcc cagattgacg gccttgagga cggcatcgac gtagttgtcg
     2701 gagttgatga ggcaccagag ggacgccatg aagacgtcgg agacgaagcc ggagttcggc
     2761 acggagacgc gcgagaggtg cggcatgagc tgcagcttcg ggaggtagta gtccggaatc
     2821 tcgacgaagt ggttctcaat gagcgcttca agcgcctcgg cgggcttgtg cccctgaagg
     2881 agctcgtcga cgtagcacga gtagagacgg cagatgacga tgctgatctg gtggctgtgc
     2941 gtgagcgaac cagccgcatc gacgatctcg aggcggtcgg ggctgttgag aagcgcatag
     3001 ggaagcatgc gcatgagtgc gcctgcgccg ttgtcgagaa tgtcgccgcc gccgcagatg
     3061 tcggcgggcg tgccgctgcg atggcg
//
LOCUS       DBMDTI010000242         3062 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7150_length_3062_cov_1.661601, whole genome shotgun sequence.
ACCESSION   DBMDTI010000242 DBMDTI010000000
VERSION     DBMDTI010000242.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7150_length_3062_cov_1.661601"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="ACZPB6_09065"
     CDS             <1..501
                     /locus_tag="ACZPB6_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR1184652.1"
                     /translation="LICARMKVLARRGHPMSGRGAALTTADLDRYPMISPTVSQPSGV
                     TAPILTLNQLSSQRVLQMPYFLACAWTLLDTDAYMVVPAPLTDLLAERLPVEALEPAD
                     LFTPLWIPSFIWHARTDLDPLRQWVRAFLITSIREKWRAYLKADEGAAVQEAQRSETR
                     EMRACV"
     gene            737..2557
                     /locus_tag="ACZPB6_09070"
     CDS             737..2557
                     /locus_tag="ACZPB6_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008541202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HIR1184653.1"
                     /translation="MQKSILAAATSLAILVVSAPALASGGPSGPHVGYPVQGHIGEVI
                     VNPYKVAPLTAVIRDGGYELSDVTVRIVPKAKGQEIKYKVSRTELKTHAGVPVFGLYP
                     DWQNTVEVSYTRTHQGKSEKFTDTYRFYTPGVYTLSTGMPNQTRPFDVKVKKVDKEFQ
                     DRLYLINSQLQSTVPLGSRFTWNNPSGGAVEWAFNSNIGIIDTTGEIRWWLLDQEIGD
                     TENPWKSGYFMGFQQTEDGALTWGYGQNYAKYDLIGRKIFDRRLPAGYSDYSHSFDNA
                     QNGHSFLRVGSSDYRRPDGKRVHTVRDVIVEIDRDGRVVDDWRLMDILDPYRDNVIKT
                     LDQGAVCLNIDASKSGQTLSAEELAKQEASDKFGDITGVGAGRNWAHVNSVDYDPSDD
                     SIIISSRHQSACVKIGRDKKVKWILAAPDGWNKELAAKVLKPIDKNGKPIKCEDGKCE
                     GDFDWTWTQHTAFRIDSKSDENTVILSVFDNGDGRGLEQPPIPSMKYTRGVIYKINQK
                     NMTVQQLWEVGKELGNEYFSPVTGITQYQADKNSMVVFYSTAGLLGPSAGKDASREES
                     SHPYLCEYRWGETKPAVELQFNKLFGYQAMPISVEKAFTH"
     gene            2708..>3062
                     /locus_tag="ACZPB6_09075"
     CDS             2708..>3062
                     /locus_tag="ACZPB6_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR1184654.1"
                     /translation="MTNKPIVAALGAALIACGSAASAAETQIYGVVSTGLYYQKAEGA
                     DHATVGMAAYKQTPTDSHLGLMGREDLGNGWYAGFQMESGWSLDSGALYDESRLFNRL
                     ARLFVGNEFIEVSAGR"
BASE COUNT          597 a         1137 c          836 g          492 t
ORIGIN      
        1 ctgatctgtg cgcgcatgaa ggtcctcgcg cgccgagggc atccgatgag cgggcggggc
       61 gccgcgctca cgacggccga cctcgaccgc tatccgatga tctcgcccac ggtctcgcag
      121 ccttcgggcg tcaccgcgcc cattctcaca ctcaaccagc tctcctcgca gcgcgtcctg
      181 cagatgccgt actttctcgc ctgcgcctgg acgcttctcg acaccgacgc ctacatggtc
      241 gtccctgcgc cgctcacgga ccttctcgcg gaacgtcttc ccgtcgaggc cctcgagcct
      301 gcggacctct tcacgccgct ctggattccg tccttcatct ggcacgcgcg cacggacctc
      361 gatccgctgc gccagtgggt gagggcgttt ctgatcacga gcatccgcga gaagtggcgc
      421 gcctacctca aggccgacga aggagcggcg gttcaagaag ctcagcggag cgaaacccgg
      481 gaaatgcgcg cctgcgtctg aagtcccccg tcccgcacgc acggcgtgca acggcccctt
      541 gcaccccatg gccgccgaag gcgggaatcc gtctctaggc ggcgcgggcc gccgtttcta
      601 caattttctc cgtgttccga aagcccccta cacaggcctt cggaagcctc accccgggcg
      661 cggcgtccgt cactgcggac gcttcgcccg tacggcgctc gaatcagaga acgccggcaa
      721 ccaaacggaa agaaaaatgc agaaatccat cctcgcagcc gcgacctcac tcgccattct
      781 cgtcgtcagc gccccggcgc tcgcctccgg cggcccctcg ggcccccacg tcggctatcc
      841 cgtccagggc cacatcggcg aagtcatcgt caatccctac aaggtggctc cgctcaccgc
      901 cgtcatccgc gacggcggct acgaactctc cgacgtcacg gtccgcatcg tcccgaaggc
      961 caagggccag gagatcaagt acaaggtctc ccgcaccgag ctcaagacgc acgcgggcgt
     1021 gcccgtcttc ggtctctatc ccgactggca gaacacggtc gaggtgagct acacccgcac
     1081 ccatcagggc aagagcgaaa agttcaccga cacctaccgc ttctacaccc ccggcgtcta
     1141 cacgctctcg accggcatgc ccaaccagac gcgtcccttc gacgtcaagg tgaagaaggt
     1201 tgacaaggaa ttccaggacc gtctctatct catcaacagc cagctgcaga gcacggttcc
     1261 gctcggctcc cgcttcacct ggaacaaccc ttcgggcggt gccgtggaat gggccttcaa
     1321 ctccaacatc ggcatcatcg acaccacggg cgaaatccgc tggtggctcc tcgaccagga
     1381 aatcggcgac acggaaaacc cgtggaagtc cggctacttc atgggcttcc agcagaccga
     1441 ggacggtgcg ctcacctggg gctacggcca gaactacgcc aagtacgacc tcatcggccg
     1501 caagatcttc gatcgtcgtc tccctgcggg ctactccgac tactcgcact cctttgacaa
     1561 cgcccagaac ggccacagct tcctgcgcgt gggctcctcc gactaccgcc gtcccgacgg
     1621 caagcgcgtc cacactgtcc gcgacgtgat cgtcgaaatc gaccgtgacg gtcgcgtggt
     1681 cgacgactgg cgcctgatgg acatccttga tccctatcgc gacaacgtca tcaagacgct
     1741 cgaccagggt gccgtctgcc tcaacatcga tgcctccaag tccggtcaga ccctttccgc
     1801 ggaagaactc gccaagcagg aagcgtccga caagttcggc gacatcaccg gcgtcggcgc
     1861 tggccgcaac tgggcccacg tcaactccgt cgactacgac ccgagtgacg actccatcat
     1921 catctcctcg cgccatcagt ccgcctgcgt caagatcggc cgcgacaaga aggtgaagtg
     1981 gatccttgcc gctccggacg gctggaacaa ggaactcgcc gccaaggtcc tcaagcccat
     2041 cgacaagaac ggcaagccga tcaagtgcga agacggcaag tgcgagggcg acttcgactg
     2101 gacctggacg cagcacacgg cgttccgcat cgactccaag tccgacgaga acaccgtcat
     2161 cctctccgtc tttgacaacg gcgacggccg cggtcttgag cagccgccca tcccgtccat
     2221 gaagtacacc cgcggcgtca tctacaagat caaccagaag aacatgaccg tccagcagct
     2281 ctgggaagtc ggcaaggaac tcggcaacga gtacttcagc ccggtcaccg gcatcacgca
     2341 gtaccaggcc gacaagaact ccatggtcgt cttctactcg accgccggtc tgctcggccc
     2401 ctcggccggc aaggacgcca gccgcgagga atcctcccat ccgtaccttt gcgaataccg
     2461 ctggggcgaa acgaagcccg cagtcgaact gcagttcaac aagctcttcg gctatcaggc
     2521 catgccgatc agcgtcgaaa aggccttcac gcactaaggc ttcctgctga aacgcgcccg
     2581 gggacgcctc ctcaccccga gtgcagcggg ggcgccctcc ggggcgctcc cgcctcatga
     2641 ccccgcgccc gttctcctcg gacgggacct cccgcgggcc cccattgatc cttcctccgt
     2701 atccaacatg accaacaagc ccatcgtcgc cgcgctcggc gcggccctca tcgcctgcgg
     2761 ctccgccgcc tcagctgccg aaacccagat ctacggcgtc gtctccacag gcctctatta
     2821 ccagaaggcc gaaggcgccg accacgccac cgtcggcatg gccgcctaca agcagacgcc
     2881 caccgactcg cacctcggcc tcatgggacg cgaggacctc ggcaacggct ggtacgccgg
     2941 cttccagatg gagagcggct ggagcctcga ctccggcgca ctctacgacg aaagccgcct
     3001 cttcaaccgt ctcgcccgac tcttcgtcgg gaacgaattc atcgaagtat ccgcaggccg
     3061 ca
//
LOCUS       DBMDTI010000243         3049 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7183_length_3049_cov_2.114654, whole genome shotgun sequence.
ACCESSION   DBMDTI010000243 DBMDTI010000000
VERSION     DBMDTI010000243.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7183_length_3049_cov_2.114654"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2140)
                     /gene="hypF"
                     /locus_tag="ACZPB6_09080"
     CDS             complement(<1..2140)
                     /gene="hypF"
                     /locus_tag="ACZPB6_09080"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HIR1184655.1"
                     /translation="MTKAATDFRVRGLVQGVGFRPTVYRIALELGLSGTVWNDAEGVV
                     VHLEGEPEVLERFPEALLAGKPPLARIDAVEPAAGELQDMEGFSISATPSGGRVTTAV
                     TADACVCDACLDDIFNPNDRRWRYPFANCTHCGPRFTITRHLPYDRPQTAMAGFPMCP
                     DCRAEYEDPLNRRFHAQPIACPVCGPKLTLVTPDGTAVEGDAIRETVKALGAGRIVAI
                     KGLGGFHLACDAENPEAVAALRSRKQRDEKPLAVMVAGLASAERFAHVTDDEKVLLTG
                     PAHPIVLLKRRTGENVPALAGVADGLETIGVMLTYTPLHALIFHSFAGEPEGRGWMND
                     VLPVTLVMTSANMSGLPLCTENEEALERLSGVADLFLLSNRDIVTPCDDSVVRAGVEG
                     TSFVRRSRGYAPEAVMLPESAGAAKKPVALAFGPYLKNTACFLRDREAFLTQHVGSLS
                     NRPTVELLSRSVSHMSALFELSPEVVACDAHPDFPSTRLALEYADLHGIPCIPVHHHA
                     AHVGVVAAERGVVKPLLGLALDGVGMGADRLPWGGELLLCGPASWARLSHLESMPMPG
                     GDRAATEPWRMGVALMAMAGAGDFAEELFPGIASVSMLRKLLAGDPEKAALLGTTTSF
                     GRWFDGMGAILGLCTHQHDEATAAMRLEGAAVRGRDNPACGTLDVEAGDRMILADGTL
                     SLVPLVRRVVDERLSGTSVECLAALVENAVI"
     gene            complement(2150..2995)
                     /gene="hypB"
                     /locus_tag="ACZPB6_09085"
     CDS             complement(2150..2995)
                     /gene="hypB"
                     /locus_tag="ACZPB6_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HIR1184656.1"
                     /translation="MCTTCGCGEHNHTHMHAHVDENGNVTTHVVHDHDHEHRHDHANG
                     HHHDQDHGPAHSHAPTTSASRAISIEEDILGRNNAIAAVNRKFFREHRVLALNLVSSP
                     GSGKTELLAQTGKRASGFRTAVIEGDQQTANDAERIRATGVRALQINTGKGCHLDAAM
                     VSHALEHLVPEDESVLFIENVGNLVCPAEFDLGEAHKVVLLSVTEGEDKPLKYPDMFR
                     AADLMILTKTDLLPYVPFDAERAEENARRVNPKIRTMRVSAVSGEGMEKWLAWLAAEL
                     MLSGL"
BASE COUNT          526 a          958 c         1052 g          513 t
ORIGIN      
        1 cgatgacggc attttccacg agagccgcga gacactcgac ggatgtgcca gagagccttt
       61 cgtcgacgac gcggcgaacg agcggcacga gcgagagcgt gccgtcggca agaatcatcc
      121 ggtcgcccgc ctcgacgtcg agcgtgccgc aggcgggatt gtcgcgtccg cgcaccgcag
      181 cgccctcaag acgcatggcg gccgttgcct cgtcgtgctg atgggtgcag aggccgagaa
      241 tggcgcccat gccgtcgaac caacgtccga aggaagtggt cgtaccgagc agggccgcct
      301 tctccggatc ccccgcgagg agcttcctga gcatcgacac cgacgcaatg ccggggaaga
      361 gctcttctgc gaagtccccc gcgcccgcca tcgccatcag ggcgacgccc atgcgccagg
      421 gttccgtggc ggccctgtcg ccgcccggca tcggcatgga ctcgaggtga gagagcctcg
      481 cccacgaggc gggcccgcag agaagaagtt cgccgcccca ggggagacgg tccgccccca
      541 tgccgacgcc gtcgagcgca aggccgagca ggggcttcac aacgccgcgc tccgccgcca
      601 cgacgcccac gtgggccgca tgatggtgaa cgggaatgca gggaatgccg tgcaggtccg
      661 catattcaag ggcaagtctc gtcgagggaa agtccggatg cgcgtcgcag gcgacgacct
      721 cgggactcag ttcgaaaagg gcgctcatgt gtgagaccga gcgcgagaga agctcgaccg
      781 tcggtcggtt cgagagggat cccacgtgct gcgtgaggaa ggcttcgcga tcacgaagaa
      841 agcacgccgt gttcttgaga tagggcccga aggcgagggc gacgggcttt ttcgccgcgc
      901 ccgcagattc ggggagcatg accgcctcgg gcgcataacc cctcgagcgg cgcacgaagg
      961 acgtgccttc aacgcccgcc ctcacgaccg agtcgtcgca gggtgtgacg atgtctcgat
     1021 tggagagaag gaagaggtct gcgaccccgg agaggcgctc gagcgcctcc tcgttttcgg
     1081 tgcagagcgg cagtccgctc atgttggcgc tcgtcatcac gagcgtgacg ggaaggacgt
     1141 cgttcatcca gccgcgtccc tcgggctcgc ccgcaaagct gtggaagatg agggcgtgaa
     1201 ggggcgtata ggtgagcatg accccgatcg tctcgagtcc gtcggcaacg cctgcaagcg
     1261 caggcacgtt ttcccccgtc cttctcttca gcagcacgat cggatgcgcg gggcccgtga
     1321 gaaggacctt ctcatcgtcg gtgacgtgtg cgaagcgctc ggccgaagca aggcccgcga
     1381 ccatgacggc aaggggcttt tcatcgcgct gcttgcggct gcggagcgca gcgacggctt
     1441 ccggattctc ggcgtcacac gcaaggtgaa agccgccgag ccccttgatc gcaacgatcc
     1501 ggccggcccc gagggccttc accgtctcac ggatagcgtc gccctcgacc gccgtaccgt
     1561 cgggcgtcac gagcgtgagc ttcggtccgc agacggggca ggcgatcggc tgggcgtgaa
     1621 agcgcctgtt gagcggatct tcgtactcgg ccctgcagtc ggggcacatc ggaaagcccg
     1681 ccatggccgt ctggggacgg tcgtagggaa ggtggcgcgt gatggtgaaa cgcgggccgc
     1741 aatgggtgca gtttgcaaac ggatagcgcc agcggcggtc atttggattg aaaatgtcgt
     1801 cgaggcaggc gtcacacaca caggcgtcgg cagtgacggc agtggtcacg cggccgccgg
     1861 agggcgtggc ggaaatggag aagccctcca tgtcctgaag ttcgcccgcc gcgggctcga
     1921 cggcgtcgat gcgcgcgagc gggggcttcc cggcaaggag cgcctcggga aagcgttcga
     1981 gcacctcagg ttcgccctca aggtgcacga cgaccccttc ggcgtcgttc cagacggtgc
     2041 ccgagagccc gagctcaagc gcgatcctgt agaccgtggg cctgaagccc acgccctgca
     2101 cgagtccgcg cacgcgaaag tccgttgcgg ccttcgtcat ggccggacct cacagtcccg
     2161 agagcatcag ttcggcggca agccaggcga gccacttctc catgccttcg cccgacacgg
     2221 ccgagacacg catcgtgcgg atcttcgggt tcacgcgtct ggcgttctcc tcggccctct
     2281 ctgcgtcaaa cgggacgtag ggcagaagat ccgtcttcgt gaggatcatc aggtcggccg
     2341 cgcggaacat gtcgggatac ttgagcggct tgtcctcgcc ctccgtgacg gagaggagca
     2401 cgaccttgtg agcctcgccg aggtcgaact cggccgggca gacgaggttg cctacgttct
     2461 cgatgaagag cacggactcg tcttcgggca cgaggtgctc gagcgcgtgg ctcaccatcg
     2521 cggcgtcgag atggcagccc ttgcccgtgt tgatctgcag ggctcgcacg cccgtggcgc
     2581 gtatgcgctc ggcgtcgttc gcggtctgct ggtcgccctc gatcacggcc gtgcgaaagc
     2641 ccgaggccct cttgcccgtc tgagcgagaa gctcggtctt gcccgaaccg ggcgacgaga
     2701 caagattgag cgcgagcacg cggtgttcgc ggaagaactt cctgttgacg gccgcaatcg
     2761 cgttgtttcg cccgaggatg tcctcctcga tcgagatggc gcgcgaggcg gacgtcgtcg
     2821 gggcgtggct gtgagcggga ccgtggtcct ggtcatgatg gtgcccgttg gcatggtcat
     2881 gacgatgctc atgatcgtga tcgtggacga catgagtcgt tacattgccg ttctcgtcca
     2941 catgggcgtg catgtgggtg tggttgtgtt cgccgcagcc gcaggttgtg cacatctttc
     3001 ggtctccttc gcgcgcatcc cgaattggag aggcgcacaa aaactttgt
//
LOCUS       DBMDTI010000244         3047 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7190_length_3047_cov_2.280041, whole genome shotgun sequence.
ACCESSION   DBMDTI010000244 DBMDTI010000000
VERSION     DBMDTI010000244.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7190_length_3047_cov_2.280041"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(125..1132)
                     /locus_tag="ACZPB6_09090"
     CDS             complement(125..1132)
                     /locus_tag="ACZPB6_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1184657.1"
                     /translation="MSKTDDLSAWRVFVTVARSGTLSAAAKALDVEVSSISRAIGGLE
                     KALGCELIRRNMRPMKLTEAGQAALKRMETILRAHDGLMEALVNDNRALTGRVRLSSA
                     PGFATRKLVPFIREFTEAHSGISVDIQTGGTEADVAKGFVDVAVITGEPTLPGLVYVS
                     RGRNLYLPVASPDYISRNGLPVTPEQLRQHRGYIYAGPVRPETKVLCRGAVSAPVEYG
                     TAVRSTDVLAIREAVLSGMGVAADMPLVQIYEDLAEGKLVPILPGWSRPPVECYIVTN
                     RDAWHMKRVRVFFEWFAAAMRSTFAGYERAVSSFVGLPPDNPPAERTEIRFTESRKRR
                     A"
     gene            1326..2750
                     /locus_tag="ACZPB6_09095"
     CDS             1326..2750
                     /locus_tag="ACZPB6_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR1184658.1"
                     /translation="MKKDEFFWLNAVNRATVVTGLRNGQFGEDLARRAAAGIAAVEAM
                     GEADPALRVKSYIAWEPLLIKAAGQGVTAIHAGRSSQDILSTQRTAILRDETLEFAAG
                     LEDVIGDLLALAERHVDTIVPSYTNGVAAQPTSYAHHLLGIAASLLRVRERLSECHTR
                     FNMCAMGATVLNGTGWPLDRDAMAAALGFPRPVENALDATSLAPVDMPLEVASALAAA
                     AVRIGSMVDDIMVQYAQPRPWIILQEGGENTYISSAMPQKRNPGLMNNCREDCSDVIG
                     EMNAAFLRAHNVVPGMIDGKRVEKNARMMKTAMTMLTRFRKVLAALTINPARALEELN
                     SDWTASQEIADRLMRDHGLPFRIGHHMASRMVGWARANNVLPLEFPYAQMRRIYREEV
                     SEEYPEGPAELPMSEEEFRAALDPRSIVAARRTAGSASPAEVRKMLEADAGRLAALRA
                     EREAEEARVAKALEDLDEAFRKLL"
     gene            2793..>3047
                     /locus_tag="ACZPB6_09100"
     CDS             2793..>3047
                     /locus_tag="ACZPB6_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HIR1184659.1"
                     /translation="MQTLMPWIAVAVTIVAGWALIKRYQTHMVLLFAGLVLLLCAAGL
                     TDASFLPKGVKPSGFFLFDIFNTLKSIATKQSAGIGFLIMT"
BASE COUNT          554 a         1063 c          936 g          494 t
ORIGIN      
        1 gggctggaaa gacacacgcc tttcatcggg gacaacaaat agattggaag agagaggttt
       61 gccgcgttcc tccatccgaa tgaattcgga ggcttcctgc gactgtttct gtgaatccga
      121 tccctcatgc acggcgcttg cgggattccg tgaaccggat ttccgttctc tctgcaggcg
      181 gattgtcggg cgggagcccc acgaaggagc tcacggcccg ctcgtagccc gcaaatgtgc
      241 tgcgcatggc ggccgcaaac cactcgaaga agacgcgcac gcgcttcatg tgccaggcat
      301 cgcggttcgt cacgatgtag cactcgacgg gcggacggct ccagcccgga aggatcggca
      361 cgagcttgcc ttcggcaaga tcctcgtaga tctgcacgag cggcatgtcg gccgcaacgc
      421 ccatgccgga gagcaccgcc tcccggatcg caagcacgtc ggtcgagcgc acggccgtgc
      481 cgtactcgac gggcgcgctc accgcgcctc tgcagagcac cttcgtctcg ggacggacgg
      541 gacccgcgta gatgtagccg cggtgctgcc tgagctgctc gggcgtgacg ggcaacccgt
      601 tgcggctgat gtagtcggga ctagcgacgg gaagatagag atttctgccg cgactcacgt
      661 agacgaggcc cggcaaggtc ggctcgcccg tgatgacggc cacgtcgaca aagcccttcg
      721 cgacgtcggc ctcggtgccg cccgtctgga tgtcgaccga aatcccggag tgcgcttcgg
      781 tgaactcgcg gatgaagggc acgagctttc tcgtcgcaaa gcccggtgcg gacgagagcc
      841 tcacgcggcc cgtgagcgcg cggttgtcgt tgacgagcgc ctccatcaga ccatcgtgcg
      901 ctctgagtat cgtctccatg cgcttcaggg cagcctgtcc cgcctccgtc agcttcatcg
      961 gccgcatgtt gcggcggatg agctcgcagc cgagcgcctt ctcaagcccg ccgatcgcgc
     1021 gggaaatgct cgagacctcc acgtcaagcg ccttggcggc cgcgctgagc gtgccgcttc
     1081 gcgcgacggt cacgaagacg cgccacgccg agaggtcgtc cgtcttgctc atgatgaact
     1141 ccggaaatcc tgatgatgca cattccgatc atagccgagc gtcgtctctg agtgacacaa
     1201 cactttgcgt ttgacgcaaa gtcattttgc gttgtgcatg gttgtgcgcc accttgcgcg
     1261 gcggcagact tgaagcatca ccaacaccgg gcggccgcat ccggaccgcc caaaaggacc
     1321 tgaaagtgaa aaaggacgaa ttcttctggc tcaacgccgt caaccgcgcg acggtcgtga
     1381 cgggccttcg caacggccag ttcggcgagg acctcgcccg cagggcggcc gccggcattg
     1441 cggccgtcga ggccatgggc gaggcggatc ccgcgcttcg cgtcaaaagc tacatcgcat
     1501 gggagccgct tctcatcaag gccgcgggcc agggcgtgac cgccattcac gcgggccgct
     1561 cgagccagga catcctctcg acgcagcgca cggcgatcct tcgcgacgaa acgcttgagt
     1621 ttgcggcagg cctcgaggac gtgatcggcg acctgcttgc gcttgccgaa cgccacgtcg
     1681 acacgatcgt gccgagctac acgaacggcg tcgccgcgca gccgacctcc tacgcccatc
     1741 atctcctcgg catcgccgcc tcgctccttc gcgtgcgcga acgcctctcc gaatgccaca
     1801 cccgcttcaa catgtgcgcg atgggcgcca ccgtcctcaa cggcacgggc tggccccttg
     1861 accgcgacgc gatggccgcc gcgctcggct ttccgcgtcc cgtcgagaac gcgctcgacg
     1921 cgacgagcct cgcgcccgtg gacatgccgc tcgaggtcgc ctcggcgctc gccgccgcgg
     1981 ccgtgcgcat cggctcgatg gtcgacgaca tcatggtcca gtacgcacaa ccgcgcccct
     2041 ggatcatcct gcaggaaggc ggcgagaaca cctacatctc ctcggccatg ccgcaaaagc
     2101 gcaatcccgg cctcatgaac aactgccgcg aggactgctc ggacgtcatc ggcgagatga
     2161 acgcggcctt cctgcgcgcg cacaacgtcg tgcccggcat gatcgacggc aagcgcgtcg
     2221 agaagaacgc ccgcatgatg aagaccgcca tgacgatgct cacgcgcttt cgcaaggtgc
     2281 tcgcggcgct cacgatcaat cccgcccgcg ccctcgagga actcaacagc gactggacgg
     2341 cctcccagga aatcgccgac cgcctgatgc gcgaccacgg tctgccgttt cgcatcggcc
     2401 accacatggc gagccgcatg gtcggctggg cccgcgccaa caacgtgctc ccgctcgaat
     2461 tcccctatgc gcagatgcgc cgcatctacc gcgaggaggt ctccgaggaa taccccgaag
     2521 gccccgccga gcttccgatg agcgaggagg aattccgcgc cgcgctcgac ccgaggagca
     2581 tcgtcgccgc ccgccgcacc gccggcagcg cctcgcccgc cgaggtgagg aagatgcttg
     2641 aggccgacgc aggcaggctc gccgccctgc gcgccgaacg cgaggccgaa gaggcccgcg
     2701 tcgcgaaggc cctcgaggac ctcgacgaag ccttcaggaa gcttctctga agccagagct
     2761 gaaaacgtcc gaacaaactg gagaacaaca tcatgcaaac actcatgccc tggatcgcgg
     2821 tcgccgtgac gatcgtcgcc ggctgggcgc tcatcaagcg ctaccagacg cacatggtcc
     2881 tgctcttcgc gggcctcgtc ctgctgctct gcgccgcagg cctcaccgac gcgagcttcc
     2941 tgcccaaggg cgtgaagccc tcgggcttct tcctcttcga catcttcaac actctgaaga
     3001 gcatcgccac gaagcagagc gccggcatcg gctttctcat catgact
//
LOCUS       DBMDTI010000245         3038 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7218_length_3038_cov_3.022131, whole genome shotgun sequence.
ACCESSION   DBMDTI010000245 DBMDTI010000000
VERSION     DBMDTI010000245.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7218_length_3038_cov_3.022131"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="ACZPB6_09105"
     CDS             <1..594
                     /locus_tag="ACZPB6_09105"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1184660.1"
                     /translation="KQICNSPSLYEKRHPRLPDSGKADSLFELVEECRENGHKMLVFT
                     QYAEMGERLQDWFEKATGVRPDFLHGGVSIAKRAEMVDRFQNDPADALLIISLKAGGT
                     GLNLTAASVVVHYDLWWNPAVENQATDRAFRIGQCRDVLVYRFLCAGTFEERINDMLE
                     RKRELADLTVATGEGWVGDLSTDELKALFRLESPENA"
     gene            703..778
                     /locus_tag="ACZPB6_09110"
     tRNA            703..778
                     /locus_tag="ACZPB6_09110"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:736..738,aa:Gly,seq:gcc)
     gene            816..891
                     /locus_tag="ACZPB6_09115"
     tRNA            816..891
                     /locus_tag="ACZPB6_09115"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:849..851,aa:Gly,seq:gcc)
     gene            1208..1390
                     /gene="rpmF"
                     /locus_tag="ACZPB6_09120"
     CDS             1208..1390
                     /gene="rpmF"
                     /locus_tag="ACZPB6_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR1184661.1"
                     /translation="MAVQQNKKSTNKRGNHRAHDFLTNPPTAIEATTGEVHRRHHISP
                     TGFYRGKKVIATKADE"
     gene            1748..2914
                     /locus_tag="ACZPB6_09125"
     CDS             1748..2914
                     /locus_tag="ACZPB6_09125"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1184662.1"
                     /translation="MDLASIVRSKADFLIGMRRRIHAHPELSGREFETAALVREELTK
                     AGIEWRPCGLQTGTLAEIRGAKPGRTVLLRADMDALPVTETTGASFASCNPGVMHACG
                     HDCHTAMLLTAALVLQETREEWGGVVRLAFQPAEESGEGALSMIAQGALEGVYACFAM
                     HVWSDVPAGRIGLISGPCMAGTDRFEIDVKGVGGHAAQPEHCVDALVAGAAIVDGLQT
                     LVSREVSPVDTAVVTIGTFNSGTRWNVIAGEARLTGTVRTLRPETAARMPEAVGRIAA
                     TIAASRRAEAVVRYEQKALPTVNDPAVTQVAREAALKVLGPDALYEIGPSMVGEDFCH
                     FAAGAPGCMGLLGVRNEACGAVYGQHHSSYTVDEDALSGGVAMYVQTAIDLAAR"
BASE COUNT          592 a          921 c          940 g          585 t
ORIGIN      
        1 aagcagatct gcaactcccc gtccctttat gaaaagaggc acccgcggct tcccgacagc
       61 ggcaaggccg actcgctctt cgagcttgtc gaggagtgca gggagaacgg gcacaagatg
      121 ctcgtcttca cgcagtacgc cgagatgggc gagaggttgc aggactggtt cgagaaggcg
      181 acgggcgtca ggcccgactt cctccatggt ggcgtctcca tcgcgaagcg cgccgagatg
      241 gtagaccgct tccagaacga tcccgcggac gcccttctca tcatctcgct caaggcgggc
      301 ggcacgggcc tcaacctgac ggcggcctcc gtcgtcgtgc actacgacct gtggtggaac
      361 cccgccgtcg agaatcaggc gaccgaccgc gccttccgca tcggccagtg cagggatgtg
      421 ctcgtctacc gcttcctctg cgcggggacc ttcgaggaac gcatcaacga catgctcgag
      481 cgcaagcgtg agctcgccga cctgacggtt gcgacaggcg aaggctgggt gggcgatctg
      541 agtacggacg agctgaaggc gctcttccgc ctcgaatcgc ctgaaaacgc ctgaaaaagg
      601 atcttcgaat accgtgttta tggtattttt cgtttttttt ggaagagtgc tcttgtcaat
      661 cccgaaaaac tgtgtaaaat tcaaatcctt cgcaggacga atgcgggagt agctcagttg
      721 gtagagcgca accttgccaa ggttgaggtc gcgagttcga gactcgtctc ccgctccatt
      781 atctctttga gagggtgtgg gttctgtcaa aaaatgcggg agtagctcag ttggtagagc
      841 gcaaccttgc caaggttgag gtcgcgagtt cgagactcgt ctcccgctcc aggtttcaga
      901 ggaaagggga agcgtgatgc ctcccctttt ttcatgcgca tccgaaacgc aaatcaaaaa
      961 aatgcgttag acagatgcca accttcggcg aatttggtaa aattcgccac tttccacgat
     1021 gggatacggt ttcgactgaa ctgccgccac gggtggttgg ttgtgccggc cccttcaaac
     1081 ggttgattgc caacggtttg aaagcgtccc gacgcggaga tttttccgga agtctgcagg
     1141 ttagttatcg agtgccgtcc tgcagaccac tcgtaatgtc actcgttttg tatacgtggg
     1201 gtttgccatg gctgttcagc agaacaagaa atccaccaac aagcgcggta atcatcgtgc
     1261 tcacgacttt ttgaccaacc cgcccaccgc gatcgaagct acgaccggtg aagtgcatcg
     1321 ccgtcaccac atcagcccga ccggtttcta ccgcggcaag aaggtgattg cgacgaaggc
     1381 tgacgaataa ttagcgtctg ccggtctttt cgacaaagaa gcttccttcc gaagccgttg
     1441 gcagcgccat gccgtccggc aagtctttgc gcgcaggatg caacgcgcga accccacggt
     1501 gagtgacaga tcaagctgac tcgcgtgaag gtttcaagga ttaggctcca ctgcagcttg
     1561 catcggaaga cgttgaaaag atcgtcgaag tcatagaaag agggatccgt caagacgggt
     1621 cccttttttt atgtcccttg ccccccatac ggctccgtcg caccgatgcg tccatggcgt
     1681 atgatcgcct catgcgcggg ccgacgagag gcccgtgact ccagatacgc acgaaaggac
     1741 acgcaacatg gacctcgcat cgattgttcg cagcaaggcg gacttcctga tcggcatgcg
     1801 ccgccgcatt cacgctcatc ccgagctgag cggccgcgag tttgaaaccg cagccctcgt
     1861 tcgggaggag ctcacgaagg cgggcatcga atggaggccc tgcgggcttc aaacgggcac
     1921 gcttgccgag atccggggcg cgaagcccgg caggaccgtt cttcttcgtg ccgacatgga
     1981 tgcgcttccc gtcaccgaga ccacgggagc ctctttcgca agctgcaatc ccggcgtcat
     2041 gcacgcctgc ggacacgact gccacacggc gatgctgctc accgccgcac tcgtgctgca
     2101 ggagacgcgg gaggaatggg gcggcgttgt caggctcgcc ttccagcctg cagaggagtc
     2161 gggtgagggc gcactctcca tgatcgccca aggggcgctc gagggcgtct acgcctgctt
     2221 cgcgatgcac gtctggtcgg acgtccccgc aggacggatc ggtctcattt ctggtccctg
     2281 catggcgggc accgacaggt tcgagataga cgtcaagggc gtgggcggtc atgccgcgca
     2341 acccgagcac tgcgtggacg cgctggtggc gggcgccgcg atcgtcgacg gactgcagac
     2401 cctcgtgagt cgcgaggttt cgcccgtcga cacggctgtc gtgacgatcg gcacgttcaa
     2461 ttcaggcacc cgctggaacg tgattgcggg cgaggccaga ctcacgggta ccgtcaggac
     2521 gctgaggcct gagaccgcag cgcgcatgcc cgaggccgtt ggtcgcattg ccgcaacgat
     2581 cgccgcctcc cgtcgcgccg aggccgtcgt gcgctatgag cagaaggcgc ttcccacggt
     2641 gaacgatccc gcggtgacgc aggttgcccg cgaggccgcc ctcaaggtgc tcggacccga
     2701 cgctctctac gagatcggcc cctcgatggt cggcgaggac ttctgtcact ttgcggcggg
     2761 cgcgcccggc tgcatgggcc tcctcggcgt gaggaacgag gcctgcggcg ccgtctatgg
     2821 acagcaccac tcgtcataca cggtcgacga ggacgcactt tcgggcggcg tcgccatgta
     2881 cgtgcagacc gccatcgacc ttgcggcccg ctgaggggcc aggcgcctcc cgtgcgctgg
     2941 gaggtagaat tgatgcttcc attgaggtaa ttccgaggag acgtcccatg accatgatga
     3001 accgccgcag cctgctcgcc gcaggctgtg cagccgcc
//
LOCUS       DBMDTI010000246         3028 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7248_length_3028_cov_2.614964, whole genome shotgun sequence.
ACCESSION   DBMDTI010000246 DBMDTI010000000
VERSION     DBMDTI010000246.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7248_length_3028_cov_2.614964"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            146..1519
                     /gene="dnaX"
                     /locus_tag="ACZPB6_09130"
     CDS             146..1519
                     /gene="dnaX"
                     /locus_tag="ACZPB6_09130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR1184663.1"
                     /translation="MSSYQVLARKWRPHDFDTIVGQEHVVTALTRALTEKRLHHAYLF
                     TGTRGVGKTTISRIFAKALNCQGADGHGDMTAHPCGVCPACRAIDEGRFPDYIEMDAA
                     SNRSVEDMTALLERAMYAPTQGRFKVYMIDEVHMLSSTAFNAMLKTLEEPPEYVKFIL
                     ATTDPQKVPITVLSRCLQFNLRNMTPQDVVGHMSRILTEEGIPFEEGALRVLAQGARG
                     SMRDGLSLLDQAIAYCGDGVTQEGVRRMLGMSGGETLTGILRALAAGDGAGMLAVADA
                     MQTQGLSFAQALRDLAGLLHRVALVQRVPAAVSEDDPDCASIREFAQAFSPEEIQLYY
                     QIALHGRNDLNLAPDEYAGFTMALLRMLAFKPAGARAVTRVPARDAGRAPVQAPASVQ
                     APASAPQAPAAVPAPAAPSAGVQTVGRSSGMPPLPRPSAPPPGLAGPAAGQLDDDTPP
                     WGEPSAS"
     gene            1647..1946
                     /locus_tag="ACZPB6_09135"
     CDS             1647..1946
                     /locus_tag="ACZPB6_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184664.1"
                     /translation="MLELTRSHVRLRLRVAAFATETGRELLSRALSSYFGSHCMVEFE
                     VGDVAGGTVADQEEREREEARRALIEGFRNDPFVKQVQALFHGTIDDTTVKANTD"
     gene            1982..2308
                     /locus_tag="ACZPB6_09140"
     CDS             1982..2308
                     /locus_tag="ACZPB6_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR1184665.1"
                     /translation="MRGFGGGMGGLMAQAQKMQAKIAKVQEEIAAMEVAGESGGGAVK
                     VVITGKHECKSVVIDPSVVDPEDVEMLQDLIQLAFNSAQTRLSEISEERMRKEVPLPP
                     GMKLPF"
     gene            2394..>3028
                     /locus_tag="ACZPB6_09145"
     CDS             2394..>3028
                     /locus_tag="ACZPB6_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013857.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR1184666.1"
                     /translation="MLRIPQSYIDELLEGDWALHDVTTEGMGLKGEYVVTAWPKETGI
                     VAGIEIAERVFKTVGLRTEMLYGDGDHAFMHSPILRAWGTPEQLHATYKVAQCVMEYS
                     TGIARRTHEMVSIARDRWGRIQVAGTRKHFPGGKILSIAALRAGGGIIHRAGISDSIL
                     VFDQHRTLTRNPVHAVRRLMAMEPERKVCVEVDTPEDGMFWADKGVHIIQC"
BASE COUNT          559 a         1033 c          931 g          505 t
ORIGIN      
        1 taaaatcaag caaattaagc gacgagctta tggcttccga gacgatctgt actttttctt
       61 aaaagtcaag gccgcgttcc acggacttcc gagatgaacc taaaataaag gttcttgcac
      121 attcactcag cactaaggac acaggatgag cagctatcag gtgctcgcca gaaagtggcg
      181 gcctcatgac ttcgacacga tcgtcggcca ggagcacgtc gtgaccgcgc tcacgcgcgc
      241 tctcacggaa aagcgccttc atcacgccta tctcttcacg ggaacccgcg gcgtcggcaa
      301 gacgacgatc tcccgcattt ttgccaaggc cctcaactgc cagggggcgg acggccacgg
      361 cgacatgacg gctcatccct gcggagtctg ccccgcctgc cgcgcgatcg acgagggacg
      421 cttccccgac tacatcgaga tggacgcggc gagcaaccgt tcggtcgagg acatgaccgc
      481 gctccttgag cgcgcgatgt acgcgcccac gcagggccgc ttcaaggttt acatgatcga
      541 cgaagtccac atgctgagct cgacggcctt caacgccatg ctcaagacgc ttgaggagcc
      601 gcccgaatac gtgaagttca tcctcgcgac gacggacccg cagaaggtgc ccatcaccgt
      661 gctctcgcgc tgtttgcagt tcaatctgcg caacatgacg ccccaggacg tcgtgggcca
      721 catgagccgc atcctcaccg aagagggcat tccgttcgag gaaggcgcgc tgcgcgtgct
      781 cgcccagggc gcccgcggct ccatgcgaga cggcctttcg cttcttgacc aggccatcgc
      841 ctactgcggc gacggcgtga cgcaggaagg tgtgcgccgc atgctcggca tgagcggcgg
      901 cgaaacgctc acgggcattc tcagggcgct cgccgcaggt gacggcgcgg gcatgctggc
      961 cgtcgccgac gccatgcaga cgcagggcct ttcctttgcg caggcgctcc gcgacctcgc
     1021 gggcctgctg caccgcgtcg cgctcgtgca gcgtgtgccc gcagccgtga gcgaggacga
     1081 tcccgactgc gcctcgatcc gagagttcgc gcaggccttc tcccccgagg agatccagct
     1141 ctactaccag atcgcccttc acggccgcaa cgacctcaac ctcgcccccg acgaatacgc
     1201 gggcttcacg atggctctgc tgcgcatgct cgccttcaag cccgcaggcg cgagagccgt
     1261 gacgcgcgtg cccgcccgtg atgcgggcag ggcgcccgtt caggcgcccg cctccgtgca
     1321 ggccccggcg tccgctccgc aggcccctgc ggcagttcct gctcccgctg cgccctcggc
     1381 tggagtacag accgtgggcc gaagctcggg catgcctccc ctgccgcgtc cctcggcgcc
     1441 gcctcccgga ctcgcgggcc ccgccgccgg gcagctcgac gacgacacgc ctccctgggg
     1501 agaaccctcc gccagttgag tacgctccgg agattcccca gagcggtccc gatccggacc
     1561 gctcggagtc ttctctcgac tggcggtcga tcattgcagg agcaggactg acgggaccgc
     1621 tgcgcaacct cgccgcttcg gcgcaggtgc ttgagctcac ccgaagccac gtcaggcttc
     1681 gtcttcgcgt cgcggcattc gcgaccgaaa cgggccgcga gcttctctcc cgcgcgcttt
     1741 cgtcctattt cggaagccac tgcatggtgg agttcgaggt gggcgacgtg gcgggcggca
     1801 ccgtggccga tcaggaggag cgcgagcgcg aggaggcccg cagggccctc atcgaagggt
     1861 tccgcaacga ccccttcgtg aagcaggtgc aggcgctgtt tcatggtacg attgacgaca
     1921 ctaccgtcaa ggcgaacacc gactgacggc ttgaatgatt ttttgaataa aggaacacaa
     1981 tatgcgtgga ttcggcggcg gcatgggcgg tctcatggct caggctcaga agatgcaggc
     2041 caagattgcc aaggtgcagg aggaaatcgc tgcgatggaa gtcgcgggtg agtccggcgg
     2101 cggtgccgtc aaggtcgtca tcaccggcaa gcacgagtgc aagagcgtcg tgatcgatcc
     2161 ctcggtcgtg gatcccgagg acgtcgaaat gcttcaggac ctgatccaac tcgccttcaa
     2221 cagcgcgcag acccgtctct ccgaaatctc cgaggagcgc atgcgcaagg aagttccgct
     2281 tcctccgggc atgaagctgc cgttctgatc aacctgacac gatccgaacg ctgacaagcc
     2341 aacagccgtc gatgccgaaa ggatcggcgg cttttttctt caggagctgc aagatgctgc
     2401 gaattccaca gtcctacatt gacgagctgc ttgagggcga ctgggccctt catgacgtca
     2461 cgaccgaagg catgggcctc aagggcgagt acgtcgtcac cgcctggccc aaggagaccg
     2521 gcatcgtcgc cggcatcgag atcgccgagc gcgtcttcaa gaccgtcggc ctcaggaccg
     2581 agatgctcta cggagacggg gaccacgcct tcatgcactc tccgatcctc cgcgcctggg
     2641 gcacgcccga gcagcttcac gccacctaca aggtcgccca gtgcgtgatg gagtactcca
     2701 cgggcatcgc ccgccgcacc cacgaaatgg tgagcatcgc gcgcgaccgc tggggccgca
     2761 tccaagtggc gggtacccgc aagcacttcc ccggcggcaa gatcctctcg atcgccgcgc
     2821 tcagggcggg cggcggcatc atccaccgcg cgggcatctc cgactccatc ctcgtcttcg
     2881 accagcacag aaccctcacg cgcaatcccg tccatgcggt ccgccgcctc atggcgatgg
     2941 agcccgagcg caaggtctgc gtcgaggtcg acacgcccga ggacggcatg ttctgggccg
     3001 acaagggcgt gcacatcatc cagtgcga
//
LOCUS       DBMDTI010000247         3012 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7293_length_3012_cov_2.489523, whole genome shotgun sequence.
ACCESSION   DBMDTI010000247 DBMDTI010000000
VERSION     DBMDTI010000247.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7293_length_3012_cov_2.489523"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="ACZPB6_09150"
     CDS             complement(<1..721)
                     /locus_tag="ACZPB6_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR1184667.1"
                     /translation="MLMFVIFGAVLLLMLLGVSTAVTMGFASIATFLFAGGDPARLFL
                     VPQRMFAQVSGITLMSIPFFLLMGNLMTVGGVSKSLFGFGRVCLGHRWGGLSNAAIVA
                     CVVMAAMSGSAAACAAGIGMIAINEMTKSGYGRPFSCATIAAGGALGPIIPPSITLIL
                     YAGLTQTSVNSLFAAGAVPGLLLGIMFMGWSSWVSARRNYAKTEPAPWRERLQAFREA
                     FFALMTPVIVMGGMFGGIFTAT"
     gene            complement(738..1739)
                     /locus_tag="ACZPB6_09155"
     CDS             complement(738..1739)
                     /locus_tag="ACZPB6_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR1184668.1"
                     /translation="MPNFIKTAAVAVAAALACAVAGAADFSAQTIRLAHTAAVSHAHH
                     EAALQFAKNVSERTGGKVKVEVYPAGELGDQPALAEQVTLGALDSAVVSLGNLAMYSK
                     KLNAMTAPFLFKDYDHAHRTIDAFVMPWMNEGLAQHDAVGLSMFDYGFRQTTTKGVPV
                     NSAADLKGVKIRVPPATGLLAAFDAVGANTQKIAYSELYTSLKQGVVTGEENPVFTIL
                     ADSLFETQDELALTNHYFDCQVLLFNKMFLEGLQPELQAILKEEAVKAQNLTRDRISH
                     GEAAVIEELKKKGMHVTNPDRDSFVKLMKPAYDKIGELAGKDEMKKLLDAVEANRAK"
     gene            2211..>3012
                     /locus_tag="ACZPB6_09160"
     CDS             2211..>3012
                     /locus_tag="ACZPB6_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HIR1184669.1"
                     /translation="MFETQYEALRRQGISRRSFLQFCSLTAASLGIGSAGAKDIAEAM
                     ETKPRTPVIWLHGLECTCCTESFIRSYHPVAKDLVLSMISLDYDDTIMAAAGHQAEAA
                     LEDTIEKYKGNYILAVEGNVPLNDDGVNCIPGGETFLRKIKHVAAGAKAVIGWGSCAA
                     WGCVQAAKPNPTHAVPITEIITDKPIVLVPGCPPIPEVMTGVVTYILTYDRLPPLDRM
                     GRPKMFYGQRIHDKCYRRAHFDAGQFVEKFDDEGAKLGYCLYKVGCKGP"
BASE COUNT          550 a          909 c         1033 g          520 t
ORIGIN      
        1 cggtcgccgt gaagatgccg ccgaacatgc cgcccatcac gatgacgggg gtcatgagcg
       61 cgaagaaggc ctccctgaag gcctgaaggc gctcgcgcca tggcgcgggt tcggtcttgg
      121 cgtagtttcg gcgcgcggag acccaggagg accagcccat gaacatgatg ccgagcagga
      181 ggcccggcac ggcgcccgcc gcaaagagcg agttgacgct cgtctgtgtg aggcccgcgt
      241 agaggatgag cgtgatcgag ggcgggatga tggggccgag ggcgccgccc gccgcgatcg
      301 tcgcgcagct gaacgggcgc ccgtagcccg acttcgtcat ttcgttgatg gcgatcatgc
      361 cgatgcccgc cgcgcaggcg gccgccgagc cgctcatggc ggccatgacg acgcaggcga
      421 cgatggcagc gttggagagt ccgccccagc ggtggccgag gcagacgcgt ccgaagccga
      481 agagcgactt gctcacgccg cccacggtca tcaggttccc catgagaagg aagaacggaa
      541 tcgacatgag cgtgatcccc gagacctgcg cgaacatacg ctgcggcacg aggaagaggc
      601 gcgcggggtc gccgcccgcg aagaggaagg tcgcgatcga ggcgaacccc atggtcacgg
      661 ccgtgctgac gccgagaagc atcagcagga gcaccgcccc gaaaatgacg aacatcagca
      721 tggatgagct tccgtgctta cttggcgcgg ttggcctcga cggcgtcgag gagcttcttc
      781 atctcgtcct tgcccgcgag ctcgccaatc ttgtcgtagg cgggcttcat gagcttcacg
      841 aaggagtcgc ggtcggggtt cgtcacgtgc atgcccttct tcttgagctc ctcgatgacg
      901 gcggcttcac cgtggctgat gcggtcgcgg gtgaggttct gcgccttgac ggcctcttcc
      961 ttcaggatgg cctgcagttc gggctggagg ccctcgagga acatcttgtt gaagagaagg
     1021 acctggcagt cgaagtagtg gttcgtgagc gcgagctcgt cctgggtttc aaagagcgag
     1081 tcggcgagaa tcgtgaagac ggggttttcc tcgcccgtca cgacgccctg cttgaggctg
     1141 gtgtagagct cggagtaggc gatcttctgc gtgttggcgc cgacggcgtc gaaggcggcg
     1201 agaaggcccg tggcgggcgg aacgcggatc ttgacgccct tgaggtcggc ggcggagttg
     1261 acgggcacgc ccttggtcgt cgtctggcgg aagccgtagt cgaacatgga gaggccgacg
     1321 gcgtcgtgct gggcgaggcc ctcgttcatc cagggcatga cgaaggcgtc gatcgtgcgg
     1381 tgggcgtgat catagtcctt gaagaggaac ggagccgtca tggcgttgag cttcttcgag
     1441 tacatggcga ggttgccgag gctcacgacc gcgctgtcga gcgcgccgag cgtgacctgt
     1501 tcggcaagcg cgggttggtc gccgagctcg cctgcggggt agacctcgac cttgaccttg
     1561 ccgcccgtgc gctcggagac gttcttcgcg aactgaagcg cggcctcgtg gtgggcgtgc
     1621 gagacggccg cggtgtgggc gaggcggatc gtctgggcgg agaagtcggc ggcaccggcg
     1681 acggcgcacg caagggcggc cgcgacggcc acggcggcgg tcttgatgaa gttgggcatt
     1741 gtgtgggctc ccgctgttgt ctgtcctgaa cctccgccca tgggcgaagg cacatgtcgt
     1801 ttttttgaag ctaaccatta taacgggagc cgcctgcggc cgaaagacgg tgataacgcg
     1861 cgcccgcaac agattcgcga ggctctccat atgcagacga ctgccgcaga gggatgaaga
     1921 ataggaggac aatttcatgt catcgtatgg gagcacgtac cgtatgaaga tttcgcggac
     1981 catatgataa ttcccccgag gtagtacttt gggggtaggc ctcatatgtc caatgcgcga
     2041 cttttgccgt atgagaggcg cttcggggac gtttagactg tgctcaaacg gtcgcaaaag
     2101 gcggacgggt gccctcgctc ccgcttgccg gctcagacgc acggaccgtt gaaacgacgg
     2161 gcccggcgca gcgggagagc tgcaaagagg ccctcaccgg agaagatcct atgtttgaaa
     2221 cgcaatacga agcgctgcgg cgtcagggca tttcccgacg cagcttcctg cagttctgct
     2281 ccctcacggc cgcgagcctg gggattggaa gcgcgggcgc caaggacatc gccgaggcga
     2341 tggagacgaa gccccgcacg ccggtcatct ggctccacgg ccttgaatgc acctgctgca
     2401 ccgaatcctt catccgcagc taccatccgg tcgccaagga ccttgtgctc tcgatgatct
     2461 cgctcgatta cgacgacacg atcatggccg ccgccggtca tcaggccgag gctgccctcg
     2521 aggacacgat cgagaagtac aagggcaact acatccttgc cgttgagggc aacgtgccgc
     2581 tcaacgacga cggggtgaac tgcattcccg gcggcgagac cttcctgagg aagatcaagc
     2641 acgtcgctgc gggcgccaag gccgtcatcg gctggggctc ctgcgccgcc tggggctgcg
     2701 tgcaggccgc caagcccaat cccacgcacg ccgtgccgat cacggagatc atcacggaca
     2761 agcccatcgt gctcgtgccc ggctgcccgc cgattcccga agtgatgacg ggcgtggtca
     2821 cctacatcct cacgtacgac cgcctgccgc cgcttgaccg catgggccgt cctaagatgt
     2881 tctacggcca gcgcattcac gacaagtgct accgccgcgc ccacttcgac gcgggccagt
     2941 tcgtcgagaa gttcgacgac gagggcgcca agctcggcta ctgcctctac aaggtgggct
     3001 gcaagggacc ga
//
LOCUS       DBMDTI010000248         2997 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7335_length_2997_cov_2.564572, whole genome shotgun sequence.
ACCESSION   DBMDTI010000248 DBMDTI010000000
VERSION     DBMDTI010000248.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7335_length_2997_cov_2.564572"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="ACZPB6_09165"
     CDS             complement(<1..455)
                     /locus_tag="ACZPB6_09165"
                     /inference="COORDINATES: protein motif:HMM:NF013213.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1184670.1"
                     /translation="MQGLIDTHCHLQAPAFEADLAEVVARARAAGVADMVVCAGGSDD
                     WERTACVAREHGLAYMLGIHPLLVPKAHEEDLERLSALAEASMADPHFIGIGEIGLEG
                     LVMTIDARQEHFFREQLRIAARLDLPCSIHVRKSASRLLYHLRRIRVTGV"
     gene            complement(458..1141)
                     /locus_tag="ACZPB6_09170"
     CDS             complement(458..1141)
                     /locus_tag="ACZPB6_09170"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1184671.1"
                     /translation="MNKELAVEVSGLVKVYHDGDHPVPVLSDVTFSVARGETVAILGS
                     SGSGKSTLLHAVGGLDTIDSGEVRVAGTSLSGLSQKALGELRNRSLGFVYQFHHLLPE
                     FTALDNVAMPLWIRREDQEASREEARRMLEAVGLSHRLQHLPSQLSGGERQRVAIARA
                     IVTKPVCILADEPTGNLDKATAQGVFDIFLKLARDEGTAIVIVTHDEGLAARCDRILR
                     LDAGVIREA"
     gene            complement(1134..2393)
                     /locus_tag="ACZPB6_09175"
     CDS             complement(1134..2393)
                     /locus_tag="ACZPB6_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HIR1184672.1"
                     /translation="MKIPFELTIGLTYTRAGRRGRRKDRFLSFISGMSVASIALGVAA
                     LIIVLSVMNGFQKEVRDRMLGVIPHIEITNGMGPMTDWRSVAADVMKNPEVVAAAPYV
                     QGQGLLNSGSVVRGAMVKGIDPETEPGVSDISQHLERVESLDVLKPGSFGILLGRDLA
                     HALRVRPGDRVSMMVPQGSVTPAGLVPRMKQFTVLGYLSSGHYEYDSTMALVNMQDAA
                     ALFRTGGPQGLRVRVTDMDLAPSVARSLARTLPPGAYASDWSRQNRTWFAAVQVEKRM
                     MGIILFLIVLVGAFGLVSSLVMTVKEKQADIAILRTLGASEGAIMRIFVIQGTIVGIV
                     GVAFGVASGLLVAENVGAIVSTIEGIFNVEFLPKSIYFISDMPSDPRMSDIIPIALFS
                     FLLSLAATLYPSWRAARTQPAEALRYE"
     gene            2465..>2997
                     /locus_tag="ACZPB6_09180"
     CDS             2465..>2997
                     /locus_tag="ACZPB6_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184673.1"
                     /translation="MSKAFILIPGARLPEGEAQRILSSLPEASARALSSLGEGAGQVV
                     AQVLDAGCYRRAPHLSWLWRVITRRNSTPHEAPWRWLSLGGREQAPELWCLTPLSLEN
                     GHVLAERPELEEREFMELTFAIEPVFLKAGLRVQIWDTSWFLTRREDWAVTTAPAMAL
                     PGLMLDEAPVEGEAAEEV"
BASE COUNT          520 a          972 c         1024 g          481 t
ORIGIN      
        1 acgcccgtga cgcggatgcg cctcagatgg tagagaagcc tcgaggcgct ctttcgcacg
       61 tgaatggagc acggcaggtc gaggcgggcc gcaatcctga gctgctccct gaagaagtgc
      121 tcctgacgtg cgtcgatcgt catgacgagc ccctcaagtc cgatctcgcc gatcccgatg
      181 aagtgcggat cggccatcga tgcctcggcg agcgccgaga gcctctcgag gtcttcctca
      241 tgcgccttgg gaacgaggag cggatgaatg ccgagcatgt aggcaagccc gtgctcacgg
      301 gcgacacagg cggtcctctc ccagtcgtcg gagccgcccg cacacaccac catgtccgca
      361 acgcctgcag cgcgggcgcg cgcgaccacc tccgcaagat cggcctcgaa ggccggcgcc
      421 tgcagatggc agtgcgtgtc gatgagtccc tgcaaggtca ggcctccctg atgacgcccg
      481 cgtcgagcct gaggatgcgg tcgcagcggg ccgcaagtcc ttcgtcgtgc gtgacgatca
      541 cgatggccgt gccctcgtcg cgcgcgagct tgaggaagat gtcgaagacg ccctgggcgg
      601 tcgccttgtc gaggttgccc gtcggctcgt cggcgagaat gcagacgggc ttcgtgacga
      661 tggcgcgtgc aatggcgacg cgctggcgct cgccgcccga gagctgcgag ggcaggtgct
      721 gcaggcggtg gctgagaccc acggcctcga gcatcctgcg ggcctcctct ctcgaggcct
      781 cctgatcctc cctgcggatc cagagcggca tcgcgacgtt gtcgagcgcc gtgaactcgg
      841 gcaggaggtg gtgaaactgg tagacgaagc cgagcgagcg gttgcgaagc tcgccgagcg
      901 ccttctggga aagccccgag aggctcgtgc ccgccacgcg aacctctccc gaatcgatcg
      961 tgtcgagccc gcccacggcg tgcagcaggg tcgacttgcc cgagcccgac gagccgagga
     1021 tcgcgacggt ctccccgcgg gccacgctga aggtgacgtc ggagaggacg gggacgggat
     1081 ggtcgccgtc gtgatagacc ttgacgaggc ccgagacctc gacggcaagt tccttattca
     1141 tagcgaagtg cctccgcagg ctgggtgcgg gccgcgcgcc agctcggata gagcgtggcc
     1201 gcgaggctca gcaggaagga gaagagcgcg atcggaatga tgtcggacat cctcggatcg
     1261 gacggcatgt cggagatgaa gtagatcgac ttcgggagga actcgacatt gaagatcccc
     1321 tcgatcgtgc tcacgatcgc gccgacgttc tcggcgacga gcaggcccga tgcgacgccg
     1381 aaggccacgc cgacgatgcc gacgatcgtg ccctggatga cgaagatgcg catgatcgcg
     1441 ccctcgctcg caccgagcgt cctgaggatg gcgatgtcgg cctgcttctc cttgacggtc
     1501 atcacgagcg aggagacgag cccgaaggcg cccacgagga cgatcaggaa gagaatgatg
     1561 cccatcatgc gcttctcgac ctgaacggcc gcgaaccagg tgcggttctg cctcgaccag
     1621 tcggaggcgt aggcgccggg cggcagcgtc ctggcgagcg agcgggcgac ggagggcgca
     1681 agatccatgt cggtgacgcg cacgcgaagg ccctggggac cgcccgtgcg gaagagcgcg
     1741 gcggcgtcct gcatgttgac gagcgccatc gtgctgtcat attcatagtg gccagaagac
     1801 aggtagccga gcaccgtgaa ctgcttcatg cggggcacga ggcccgcggg cgtgacgctg
     1861 ccctgcggca ccatcatcga gacgcggtcg ccgggacgca cgcgcagcgc atgggcgagg
     1921 tcgcgtccga gcaggatgcc gaagctgccg ggcttgagga cgtcgagcga ttcgacgcgc
     1981 tcaaggtgct gcgagatgtc gctcacgccg ggttccgtct cgggatcgat gcccttgacc
     2041 atcgcgccgc gaacgacgct gcccgagttg agcaggccct gtccctgcac gtagggcgcg
     2101 gccgcaacca cctcgggatt cttcatgacg tcggcagcga cgctcctcca gtcggtcatg
     2161 ggacccatgc cgtttgtgat ctcgatgtgc gggatgacgc cgagcatgcg gtcgcggacc
     2221 tccttctgga agccgttcat gacggagagc acgatgatga gcgcagccac gccgagcgcg
     2281 atcgatgcga cggacatgcc ggagatgaac gaaaggaagc ggtccttgcg gcgtcccctg
     2341 cgtcctgctc gcgtgtaggt gaggccgatg gtcagttcaa acggaatttt cacgggctga
     2401 caaggtgagg agttgggaag gtttccattc tagcctgtcc tcctcccgtt acaatgctca
     2461 aactatgtcg aaagctttca ttctcattcc cggcgcccgc ctgccagaag gcgaggcgca
     2521 gcgcatcctg tcctcgctcc ccgaggcgtc cgcacgtgcg ctctcgagcc tcggcgaagg
     2581 cgcgggacag gtcgtcgcac aggtgctcga cgcaggctgc taccgccgcg ccccgcacct
     2641 ttcctggctc tggcgcgtga tcacgcgccg caattccacg ccccatgagg cgccctggcg
     2701 ctggctctcc ctcggaggcc gcgagcaggc gcccgaactc tggtgcctca cgccgctctc
     2761 gctcgaaaac ggccacgttc tcgccgaacg ccccgaactc gaggagcgcg aattcatgga
     2821 gctcacgttc gccatcgagc ccgtcttcct gaaggcgggc cttcgcgttc agatctggga
     2881 cacgagctgg ttcctgacgc gtcgcgagga ctgggccgtc accacggcgc ccgccatggc
     2941 gctcccgggc ctcatgttgg acgaggcgcc cgtcgagggc gaagccgccg aagaggt
//
LOCUS       DBMDTI010000249         2990 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7358_length_2990_cov_3.123572, whole genome shotgun sequence.
ACCESSION   DBMDTI010000249 DBMDTI010000000
VERSION     DBMDTI010000249.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7358_length_2990_cov_3.123572"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="ACZPB6_09185"
     CDS             <1..263
                     /locus_tag="ACZPB6_09185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008347260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1184674.1"
                     /translation="VSRTPERLVMSVADTGPGIAPEERSRVFDPFYRVTGSGLPGTGL
                     GLAIVKTYADMTGAEVTLADTVPGGTPPGLTATVSCPLPPVG"
     gene            complement(235..1143)
                     /gene="rarD"
                     /locus_tag="ACZPB6_09190"
     CDS             complement(235..1143)
                     /gene="rarD"
                     /locus_tag="ACZPB6_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIR1184675.1"
                     /translation="MDRIGVLFTLAAYLLWGLFPLYFHALQDVSALEILAHRIVWSLV
                     VTAAILMALRRGEWIRGLVKEPRTLAIFTGSALLIGLNWGTYVYSIVSGQTIEASLGY
                     FINPLMSVALGALFLGERLSKAQLGAVALAAAGVLYITISTGTAPFLGLVLAASFALY
                     GLIRKRAPLGSLEGMALENVILAPFALGYMFWLLERGELLFATEGWDVKTLLLLAGPI
                     TTIPLLLFASGVRRIPYSTVGIIQYVSPTIVFLLGRFAFGEPFSVEMMTGFVLIWTAV
                     VVFTVDTVRTTARLRREINRRAAEDS"
     gene            1240..1503
                     /locus_tag="ACZPB6_09195"
     CDS             1240..1503
                     /locus_tag="ACZPB6_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184676.1"
                     /translation="MTSPAFHPASSLRNPTRKKYSSRTIKNFGRLDLLLENDPNALEK
                     LRLQAEQLNAERETAKQTAVQQRLTEAPRQMEIKDDTNADNIR"
     gene            1503..>2640
                     /locus_tag="ACZPB6_09200"
     CDS             1503..>2640
                     /locus_tag="ACZPB6_09200"
                     /inference="COORDINATES: protein motif:HMM:NF033559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HIR1184677.1"
                     /translation="MIGAILYRQIWNKLNLTRKLQDFQQAGKTRHDLAEAIFYMTVAR
                     SLMPDSGLAQWNKHNTFLYSGARLQLRHHLRALNELLPHKQELLAYLNRQMEKHFDRT
                     VNTAPCVVTTCRFDARDADTLRTFDSDRNNKDNQVQVTMTILIDQTGIPIDYDLFHGE
                     ASEPESLSLFLDQMTTKYGIGQVFVMADRDPNSDTSLQQILDLGPEFVINVQRAEGNP
                     KADENRIDAGSLFDRHGEMRHRLRQTEEWFRISKSLLEAHSDFHRKESRIEAHCLISC
                     LALMLHRLLEKTVRDAGENMTSKQMAEALASAELMEVLLPDGQTFYAKARACGDFERI
                     CRAVGLGVLPRLAKSADLNNLATEVGGFRYSRGFSPRGYPRFAVT"
     assembly_gap    2641..2650
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          652 a          932 c          866 g          530 t
ORIGIN      
        1 gcgtctcgcg cacgcccgag cgcctcgtga tgagcgtggc cgacacgggc cccggcattg
       61 cgcccgagga gcgttcgcgc gtcttcgatc ccttctaccg cgtcacgggt tccgggcttc
      121 cgggcacggg cctcggcctt gcgatcgtga agacctacgc cgacatgacg ggcgccgagg
      181 tgacgctcgc cgacacggtg ccgggcggca cgcctcccgg cctgacggcg acggtcagct
      241 gtcctctgcc gcccgtcggt tgatctcgcg cctcagacgg gcggtcgtgc ggaccgtgtc
      301 gaccgtgaag acgacgacgg ccgtccagat cagcacgaag cccgtcatca tctcgaccga
      361 aaaaggctcc ccgaacgcaa agcgcccgag tagaaagacg atggtcgggc tcacgtactg
      421 gatgataccc accgtcgaat aggggatgcg gcgcacgccg gacgcaaaga gaagaagcgg
      481 aatcgtcgtg atcggtcccg ccagcagaag cagggtcttc acgtcccagc cttccgtcgc
      541 aaagagaagc tcgcctctct caagcagcca gaacatgtag ccgagcgcaa acggcgcgag
      601 gatcacgttc tcaagcgcca tgccctcaag acttcccaga ggcgcgcgct ttcggatgag
      661 gccgtaaagc gcaaagctcg cggccagaac tagaccgagg aacggtgccg tccccgtcga
      721 gatcgtgatg tagaggacgc ccgcagccgc cagagccacg gctccaagct gggccttcga
      781 gaggcgctcg cccagaaaga gcgcccccag agccaccgac atcagcgggt tgatgaagta
      841 gcccagcgag gcctcgatcg tctgcccgct cacgatcgaa taaacgtagg ttccccagtt
      901 gaggccgatg agaagcgcgg agcccgtgaa gatcgcaagc gtcctgggtt ccttcacgag
      961 ccctctgatc cactctccac ggcgaagcgc catcaggatc gccgcagtca cgacgagcga
     1021 ccagacgatt cggtgcgcaa gaatttcaag ggcgctcacg tcctgaagcg catggaaata
     1081 aagaggaaag aggccccaga gaagataggc cgcgagggtg aagagaacgc cgattctgtc
     1141 catgtgacgc cgtgtttttt cttgttcgtt agaccggttg tgcgccagcg ggcgcccttc
     1201 ctagtctacc ttttcggcgc gtgcgcggcc tcaggcggaa tgactagtcc ggcttttcat
     1261 cctgcctcat ctcttcgaaa tcccacccgc aagaagtatt ccagccgtac gatcaaaaac
     1321 ttcgggcgtc tggatcttct tttggaaaac gatcctaacg ctctggagaa gttgcgcctt
     1381 caggcggagc agctcaatgc ggaacgggag acggccaagc agacagcggt tcagcaacgt
     1441 ttgaccgaag cgccccgtca gatggagatc aaggacgata cgaatgccga caacatacgg
     1501 tgatgatcgg cgccatcctg taccgtcaaa tctggaacaa gctgaatctg acccgaaaat
     1561 tgcaggattt ccaacaagca ggcaaaacgc gtcatgacct ggcggaggcg attttctaca
     1621 tgaccgtcgc ccgcagtctc atgcccgact ccggattggc gcagtggaac aagcacaaca
     1681 cctttcttta cagtggagcc cgccttcagc tccgccatca cctccgcgcc ctcaacgagc
     1741 tgctgcctca taagcaggag cttctggcct acctgaatcg ccagatggag aagcattttg
     1801 accgcaccgt gaacacggca ccctgtgtcg tgacgacctg caggttcgat gcacgggacg
     1861 ccgacacgct gcgcacgttc gactccgaca ggaacaacaa ggacaatcag gttcaggtga
     1921 ccatgaccat cctgatcgat caaaccggca ttcccatcga ttacgatctg tttcacgggg
     1981 aagcgagtga gcccgaatcc ctgagcctgt tcctggatca gatgacgacg aagtacggga
     2041 tcgggcaggt tttcgtgatg gccgatcgtg atcccaacag cgataccagt cttcagcaga
     2101 ttctcgatct cggaccggaa ttcgtcatca atgtccaacg agcggaagga aatccgaagg
     2161 ctgacgagaa tcgaatcgat gcaggcagcc tcttcgacag gcacggagag atgcgccacc
     2221 gcctccggca gactgaggaa tggttccgca tatcgaaatc gcttttggaa gcgcattcag
     2281 actttcaccg gaaggaatcc cgcattgagg cgcactgtct gatcagctgt ctggcgctca
     2341 tgcttcacag acttctggag aaaaccgtca gggatgccgg cgaaaacatg acgtcgaaac
     2401 agatggcgga ggctctggct tcggcagaac tcatggaggt cctgctgcct gacgggcaga
     2461 ccttctacgc caaggcgaga gcctgcgggg actttgaaag aatctgccgg gccgtcggct
     2521 tgggagtgct gccgagactg gcaaagtcgg ctgacctaaa caacctcgcc actgaagtgg
     2581 gagggtttcg atacagtcga ggcttttcgc ctcggggtta cccccgattt gctgtcactc
     2641 nnnnnnnnnn ggcggcgcca aggcgcgcgc agcgcttcgc aagtcgctcg aaggcgccgt
     2701 gcgcatcgat ccgggcttcc tcaacgatcc ctgcctcacc tccacccgct cgaaccccta
     2761 ctggcaggcc cccgtcaagg ccctcgtacc ctgcagcagg cgcgccgaca tcgaaatgat
     2821 ccgctaaaca acctcgccac tgaagtggga gggtttcgat acagtcgagg cttttcgcct
     2881 cggggttacc cccgatttgc tgtcactccg accacgaaat ccttgctgac gcagcccttg
     2941 tacggtaagg gacggaatcc cccgccgtcg gaacctcccg aactcgcgct
//
LOCUS       DBMDTI010000250         2987 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7372_length_2987_cov_2.080388, whole genome shotgun sequence.
ACCESSION   DBMDTI010000250 DBMDTI010000000
VERSION     DBMDTI010000250.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7372_length_2987_cov_2.080388"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="ACZPB6_09205"
     CDS             complement(<1..605)
                     /locus_tag="ACZPB6_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR1184678.1"
                     /translation="MTETAVPNVLKSEVLARFVMETPSEAVPDAMVKKAVRHILDSIG
                     AGVAGAVSVEAQRLTAVLREEGEGPGRATLWGRGETMTPVNAALVNGTASHAFELDDT
                     GGCDHSGAVVVPAAVAAVELADRPVTGREFIDAVVIGYDVARRPLEACGAYEPHNGAG
                     FHSTGTCGTFGAAAAAARILGLSVRETQNALGLASSFSSGL"
     gene            845..2182
                     /locus_tag="ACZPB6_09210"
     CDS             845..2182
                     /locus_tag="ACZPB6_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HIR1184679.1"
                     /translation="MEMDFNFWLQFAIVMVCVAIGGRLGGVGLGAAGGLGVCLLVLVL
                     GVPPGSPPISVLLIITAVIACTSVLQGSGGLDLMVRWAEKLLRRWPKMITIVGPLVCS
                     FFVMVVGTSYVAFAVYPVVAEVAASARVRPERAVSASVICAGIAVMASPMSAAMAAMV
                     GIMATQGVEFHQILAVTIPTFILSLFCAALSVFWRGKELEDDPEFKARLANGDYQWLA
                     THSTTEMKFAEAPFARRAVCIFLLGILVSILVGSVSDLRPSWTVGEKTTLLPIPNIIQ
                     MVMLATAFFIIMLCRVPSAKFASGSVFRSGLIGVVGVFGIAWCTGTFFDQHTKVLADA
                     FAGLAKDAPFVFCLATFCVSTVIFSPTVSTTIMIPLGLKFGLPPEFLVGTWACCYGDF
                     IIPGGAQIGCTAFDRTGTTRLGTFVINHSYLRPGLVFVTCGTFIGWGISKLVF"
     gene            complement(2371..2775)
                     /locus_tag="ACZPB6_09215"
     CDS             complement(2371..2775)
                     /locus_tag="ACZPB6_09215"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR1184680.1"
                     /translation="MNTRLLLGALATAAVLASPAHAKLDGKVPEPGMVTFVTFSADGC
                     QPCRLMEPALRKLDQEYEGRAAIREINTSEYPELVPYFRIAAVPTQILFGKDGKELGW
                     HQGYMDESSMRQAIEGALKAAEAKPDETKAAK"
     gene            complement(2808..>2987)
                     /locus_tag="ACZPB6_09220"
     CDS             complement(2808..>2987)
                     /locus_tag="ACZPB6_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184681.1"
                     /translation="VVSFVQETEAAKTAAQPKQAKNLFIIIRKEGKIRTQFGATVHPL
                     CAIIGKFCRKTCGRS"
BASE COUNT          461 a         1011 c          895 g          620 t
ORIGIN      
        1 cagaggccgg aggagaagga cgaggcaagg ccgagcgcat tctgcgtctc cctcacggaa
       61 aggcccagga tgcgggctgc ggctgcggcc gcaccgaacg tgccgcaggt gcccgtcgag
      121 tggaagcccg cgccgttgtg cggttcatag gcgccgcagg cctcgagcgg acggcgggcg
      181 acgtcgtagc cgatcaccac cgcgtcgatg aactcgcgcc ccgtcacggg acggtccgca
      241 agctccaccg cggcgacggc cgcaggcacc accacggcgc ccgaatggtc gcatccgccc
      301 gtgtcgtcaa gctcgaaggc atgcgaggcc gtgccgttga cgagcgcggc gttgacgggc
      361 gtcatcgtct cgccgcggcc ccagagcgtg gccctgccgg gcccctcgcc ttcttcgcga
      421 agcacggccg tcaggcgctg cgcctcaacc gagacggccc ccgcaacgcc cgcgccgatc
      481 gagtcgagaa tgtggcgcac ggccttcttc accatcgcgt cgggcacggc ttcggagggc
      541 gtctccatga cgaatcgggc gagcacctcg ctcttgagaa cattcggaac agcggtttca
      601 gtcacttcag gctcctttcg tgaattcagt cgtcagggca atccatgact gcccacgggt
      661 cacgccaata ataccgaaag cccgctgccg acgtgcatgc ggagccttcg aacacaacga
      721 gcgccaggca ccccgtaaaa accatgatgt gtgtcagtgc aacaaactgt aaaataccgc
      781 gtcgcggccc ctgactgaaa tactgccgcc tccctccaac aaccaaaaag aaagctttac
      841 aaccatggaa atggatttca acttctggct ccagttcgcc atcgttatgg tctgcgtagc
      901 catcggcggc cgtctgggcg gcgtcggcct cggtgcggcc ggcggtctcg gcgtgtgcct
      961 tctcgtcctc gttcttggcg ttcccccggg ttctcctccg atttccgtcc tgctcatcat
     1021 cacggcggtc atcgcctgta cgtccgtcct tcagggttcg ggcggtcttg acctgatggt
     1081 ccgttgggcc gagaagctcc ttcgccgctg gccgaagatg attaccatcg tggggccgct
     1141 cgtgtgctcc ttcttcgtga tggtcgtggg cacgtcctac gtggccttcg cggtctatcc
     1201 cgtggtcgcc gaagtggcgg cctcggctcg cgtgcgcccc gagcgcgccg tttcggcatc
     1261 cgtgatctgc gccggcatcg ccgtgatggc ctcgcccatg tccgccgcca tggccgccat
     1321 ggtgggcatc atggccactc agggcgtcga attccatcag attctggccg tgacgatccc
     1381 gaccttcatc ctctcgctct tctgcgccgc cctctccgtg ttctggcgcg gcaaggagct
     1441 cgaggacgac ccggaattca aggcccgcct cgccaacggc gactaccagt ggctcgccac
     1501 gcactccacg actgaaatga agttcgcgga ggctccgttt gcccgccgtg ccgtctgcat
     1561 cttcctgctc ggcattctgg tctcgattct cgtgggctcc gtctccgacc tgcgtccgtc
     1621 ctggacggtc ggcgaaaaga ccacgcttct tcctatcccg aacatcatcc agatggtgat
     1681 gctcgccacg gccttcttca tcatcatgct ctgccgcgtc ccgtccgcga agttcgcctc
     1741 gggctccgtg ttccgctccg gcctcatcgg cgtcgtgggc gtcttcggca tcgcctggtg
     1801 tacgggcacg ttcttcgacc agcacaccaa ggtcctcgcc gacgccttcg ccggcctcgc
     1861 caaggatgcg ccgttcgtct tctgccttgc taccttctgc gtctccaccg tcatcttctc
     1921 cccgacagtc tccacgacga tcatgatccc gctcggcctc aagttcggcc tgccgcccga
     1981 gttcctcgtc ggcacctggg cctgctgcta cggcgacttc atcatcccgg gcggcgcgca
     2041 gatcggctgc acggccttcg accgcacggg cacgacgcgt ctgggcacct tcgtgatcaa
     2101 ccactcctac ctgcgtccgg gcctcgtctt cgtgacctgc ggcacgttca tcggctgggg
     2161 catctccaag ctggtcttct gatcggcctg atcccgaaag tccatatcga aacgggcgtc
     2221 ctccatgccg gagggcgccc gttttcacat tcggtggaat gcacgaaggg gcgcggattc
     2281 agcatccggc gccccttcaa ttgtcccttg tcctcgtcct tcagcctccc gtccggagag
     2341 acggagagag gaaggagcgg atcctgccga ctactttgcg gccttcgtct cgtcgggctt
     2401 cgcttcggcg gccttgagag ccccctcgat cgcctgcctc atgctcgact cgtccatgta
     2461 gccctggtgc cagccgagct ccttgccgtc cttgccgaag aggatctggg tgggcacggc
     2521 cgcgatgcgg aagtacggca cgagttcggg gtattcggag gtgttgatct cgcggatggc
     2581 ggcgcggccc tcgtactcct ggtcaagctt cctgagcgcg ggctccatca ggcggcaggg
     2641 ctggcagccg tccgcgctga aggtgacgaa ggtcaccatg ccgggctcgg gcaccttgcc
     2701 gtcgagcttc gcatgagcgg gtgaggccag cacggcggca gtcgcaaggg cgccgagaag
     2761 aagtcgagtg ttcatgacga atccgtttcg tttgatgatg attgaattca agacctgccg
     2821 cacgttttgc ggcagaactt tccgatgata gcgcacaaag gatggacggt tgcgccgaac
     2881 tgcgttcgga ttttcccttc cttccgaatt attatgaaaa gatttttagc ttgttttggt
     2941 tgtgcagccg ttttggctgc ttcggtttcc tgtacgaagg atacgac
//
LOCUS       DBMDTI010000251         2983 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7381_length_2983_cov_2.800139, whole genome shotgun sequence.
ACCESSION   DBMDTI010000251 DBMDTI010000000
VERSION     DBMDTI010000251.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7381_length_2983_cov_2.800139"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(73..567)
                     /gene="lspA"
                     /locus_tag="ACZPB6_09225"
     CDS             complement(73..567)
                     /gene="lspA"
                     /locus_tag="ACZPB6_09225"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR1184682.1"
                     /translation="MTDASARPTPAKFAAWLCLGLFVLGIDQATKIWFEQNFSLGEVL
                     PVCTGFNLVLAHNTGAAFSFLADAGGWQRWAFAALAAAVIVAMIVLLVRNSHRKLLSL
                     SLTLIAAGALGNMIDRTVYGYVVDFLDFYWQGWHWPAFNVADVAICGGAFFLIVDELR
                     SGRR"
     gene            complement(591..>2983)
                     /gene="ileS"
                     /locus_tag="ACZPB6_09230"
     CDS             complement(591..>2983)
                     /gene="ileS"
                     /locus_tag="ACZPB6_09230"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR1184683.1"
                     /translation="MRHEDAWAMYQESALAAVVKKGYVYRGLKPVNWCFDCQSALAEA
                     EVEYKDKKSHTIDVAFELADEDRARVEEIFGRSLEKPCFNVIWTTTPWTIPANQALNM
                     NPELEYGLYDVGDRLLILGTGLAEAALERYGMKGEKIATAMGDKFELVRFRHPLWHVH
                     EGFRRFSPVYLADYVDATAGTGIVHSAPAYGVDDFISCKKHGMTNDQVLTPVMGDGTY
                     SESLPLFGGLNIWAAADKICETIAVAGNLLSHGSIVHSYMHCWRHKTPLVYRATNQWF
                     VRMDAPNADTRGVLDTEASERSLRDAALECVKATKFFPTWGINRLYAMIENRPDWCIS
                     RQRNWGVPLPFFMHKETGELHPRTLEIMEEVAKMVEEKGIESWATAKPEDFLTANEAA
                     DYVRSTDILDVWFDSGVTHYTVMRGSHADELAWPADLYLEGSDQHRGWFHSSLLTGTM
                     IDGRAPYKMLLTHGFVVDEKGEKMSKSKGNVVRPQEVSEKFGAEILRLWVASTDYSGE
                     LRLGQTILKRVVESYRRVRNTLRFLLANTSDFDASKDLVPVENLLELDRWALAYTERF
                     QKEVLAEYGEFRFHNVVSMLQTFASADLGSFYLDVLKDRLYTTAPGSEARRSAQTALW
                     YITKTLLRLMAPVLSFTAEEAFKVFSPNESETIFTERFEPLPEVAGAEALLAKWDAVR
                     TVRADLQKKIEELRGEGQIGSSLQAVVRIEAAPAVYDALASLGDELRFVMIVSAVELV
                     KSADEETHFAVSASKEGKCERCWHYVAGIGSDAEHPTLCPRCASNLFGAGEKRLFA"
BASE COUNT          525 a          851 c         1071 g          536 t
ORIGIN      
        1 ggtaaaagga aagggactgg aatccgtttg gtttccagtc cctcattgag gagtggtgat
       61 gcccgaaatg acttatctgc gtccgcttct cagttcgtcg acgatcagga agaaggcgcc
      121 gccgcagatg gcgacgtcag cgacgttgaa ggcgggccag tgccagccct gccagtagaa
      181 gtcgagaaag tcgacgacgt agccgtagac ggtgcggtcg atcatgttcc cgagcgcacc
      241 tgccgcgatg agcgtgagcg agaggctcag cagcttgcgg tggctgttgc gcacgagaag
      301 cacgatcatc gcgacgatga cggcggcggc gagggccgcg aacgcccagc gctgccagcc
      361 gcccgcatcg gccaggaacg aaaaggccgc gcccgtgttg tgcgccagga ccagattgaa
      421 tcccgtgcac acgggcagga cctcgccgag cgagaagttc tgctcgaacc agatcttcgt
      481 cgcctgatca atgccgagga cgaagagtcc gaggcagagc caggcggcga acttcgcggg
      541 ggtcgggcgg gcggaggcgt ccgtcatgag ggcttcctca ggccgccgca tcaggcgaag
      601 aggcgctttt caccggcgcc gaagaggttg gatgcgcagc gcgggcagag cgtcggatgc
      661 tcggcatcgc tgccgatgcc ggcgacgtag tgccagcagc gctcgcactt gccttccttc
      721 gaagcggaga cggcgaagtg cgtctcctcg tcggcggact tcacgagttc gacggcggag
      781 acgatcatca cgaagcggag ctcgtcgccg agggacgcca gagcgtcgta gacggcggga
      841 gcggcctcga tgcggacgac ggcctgaagc gaggagccga tctggccttc gccgcggagc
      901 tcttcgatct tcttctgaag gtcggcgcgt accgtgcgga cggcgtccca cttggcgagg
      961 agggcctcgg cgccggccac ttcgggcagc ggttcgaagc gttccgtgaa gatcgtttcg
     1021 ctttcgttgg gcgagaagac cttgaaggcc tcctcggccg tgaaggagag gaccggagcc
     1081 atcaggcgca ggagcgtctt cgtgatgtac cagagggccg tctgagcgga gcggcgggcc
     1141 tcggagccgg gagccgtcgt gtagagacgg tccttgagca cgtcgaggta gaagctgccg
     1201 aggtcggcgg acgcgaacgt ctggagcatc gagacgacgt tgtggaagcg gaattcgccg
     1261 tactcggcga ggacttcctt ctggaacctt tctgtatagg cgagggccca gcggtcgagc
     1321 tcgagcaggt tctcgacagg cacgagatcc ttcgaagcat cgaagtcgga cgtgttggcg
     1381 agcaggaagc gcagcgtgtt gcgcacgcgg cggtagcttt cgacgacgcg cttgagaatc
     1441 gtctggccga ggcggagctc gcccgagtag tcggtggagg cgacccagag acggaggatt
     1501 tcagcgccga acttctcgct gacttcctgc ggacgaacga cgttgccctt ggacttggac
     1561 atcttctcgc ccttttcgtc cacgacgaag ccgtgggtga gaagcatctt gtacggcgcg
     1621 cggccgtcga tcatcgtgcc cgtgaggagc gaggagtgga accagccgcg gtgctggtcg
     1681 gagccctcca ggtagaggtc ggccggccag gcgagttcgt cggcatggct gccgcgcatc
     1741 acggtgtagt gcgtgacgcc ggagtcgaac cacacgtcga gaatgtcggt ggagcggacg
     1801 tagtcggcgg cctcgttggc agtgaggaag tcctcgggtt tggcggtggc ccaggattcg
     1861 atgccctttt cctcgaccat cttcgcgacc tcttccatga tctcaagggt gcgcgggtgc
     1921 agctcgcccg tttccttgtg catgaagaag gggagcggaa cgccccagtt gcgctgacgg
     1981 gagatgcacc agtcgggacg gttctcgatc atcgcgtaga ggcggttgat gccccaggtc
     2041 gggaagaact tcgtggcctt gacgcattcg agggcggcat cgcgaaggga tctttcgctc
     2101 gcctctgtgt cgagcacgcc gcgcgtgtcg gcgttcggag cgtccatgcg gacgaaccac
     2161 tggttcgtgg cgcggtagac gagcggggtc ttgtggcgcc agcagtgcat gtagctgtgc
     2221 acgatcgagc cgtgggagag caggttgcct gcaacggcga tcgtctcgca gatcttgtcg
     2281 gccgcagccc agatgttgag gccgccgaag agcggaagcg actcggagta ggtgccgtcg
     2341 cccatcacgg gggtgaggac ctggtcgttc gtcatgccgt gcttcttgca ggagatgaag
     2401 tcgtccacgc cgtaggcggg agcggagtgc acgatgcccg taccggccgt cgcgtcgacg
     2461 tagtcggcga gatagacggg cgagaagcgg cggaagccct cgtggacgtg ccagagagga
     2521 tgacggaagc gcacaagctc gaacttgtcg cccatggcgg tagcgatctt ctcgcccttc
     2581 atgccgtagc gctcaagcgc ggcttcggcg aggcccgtgc cgaggatgag aaggcggtcg
     2641 cccacgtcgt agagcccgta ttcgagctcg gggttcatgt tcagggcctg gttggcggga
     2701 atcgtccagg gcgtcgtcgt ccagatgacg ttgaagcagg gcttttcaag gctgcggccg
     2761 aagatttcct cgacgcgtgc gcggtcctcg tcggcgagct cgaaggcgac gtcgatcgtg
     2821 tggctcttct tgtccttgta ctcgacttcg gcttcggcca gggcggactg gcagtcgaag
     2881 caccagttca cgggcttgag gccgcggtag acatagccct ttttcacgac ggcggcaagg
     2941 gcgctctcct gatacatggc ccaggcgtcc tcatggcgca tac
//
LOCUS       DBMDTI010000252         2957 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7472_length_2957_cov_2.413515, whole genome shotgun sequence.
ACCESSION   DBMDTI010000252 DBMDTI010000000
VERSION     DBMDTI010000252.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7472_length_2957_cov_2.413515"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            246..1139
                     /gene="dapA"
                     /locus_tag="ACZPB6_09235"
     CDS             246..1139
                     /gene="dapA"
                     /locus_tag="ACZPB6_09235"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR1184684.1"
                     /translation="MKFENVRGSIVAMITPFHEDGSVNFEALTHLIERQIAGGTSAIL
                     TLGTTGEYSTMTHEEDAAVVRHTIDTVAGRVPVIVGSGSNCTATQIEMSRRYEAMGAD
                     ALLIIAPYYNKANAEGMYRHFADTADAVSVPCILYNVPGRTGCSIPVSVVERLAKHPN
                     ICGIKEASGNMSYAMEVARFLGPDFQMWSGNDDITVPIMSVGGSGVISVYANIDPASC
                     AKMCTDWFAGRTAEAATEQVRRLDLMNALFYEVNPIPAKTALNMMGLNAGPMRLPLCD
                     MSPANAARLRAELVKAGLINE"
     gene            1160..1888
                     /gene="dapB"
                     /locus_tag="ACZPB6_09240"
     CDS             1160..1888
                     /gene="dapB"
                     /locus_tag="ACZPB6_09240"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR1184685.1"
                     /translation="MNIILIGTGRMGRLLKECALDAGDAVVGEYGRANIAELAAAPAA
                     DVAIDFSGPEALPAVAAYVARTGTPLVSGTTGYTAGEKAAVVALGEHAPVVWSANYSL
                     GVAVLARALRLVTAALRPDFDIEVTETHHNRKKDAPSGTAKLLVEALDPAGELMPVYG
                     REGLCGERRPDEIGIHALRGGTVAGTHSVHFFGPDEELEFTHRATSRRIFVLGALKAA
                     RALVREPKGFHSLDDLLFNTNGTH"
     gene            1893..2597
                     /gene="dapD"
                     /locus_tag="ACZPB6_09245"
     CDS             1893..2597
                     /gene="dapD"
                     /locus_tag="ACZPB6_09245"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HIR1184686.1"
                     /translation="MNAQEIINYIATAPKKTPVKIYVNLREPVDFASCGAKVFGEGLS
                     LVVFGNWADLEPVVTANAAAIADIIIENDRRNSGVPLLDIRNIRARIEPGAVIREKVE
                     IGEGAVIMMGAVINIGAVIGEGTMIDMGAVLGGRAIVGNRCHIGAGTVLAGVVEPASA
                     TPVVIEDDVMIGANAVVIEGVRVGRGAVVGAGAIVIKDVPAGAVVVGNPARIIKMKDE
                     KTEAKTALIDALRTLD"
     gene            2719..>2957
                     /locus_tag="ACZPB6_09250"
     CDS             2719..>2957
                     /locus_tag="ACZPB6_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184687.1"
                     /translation="MIRTRIEVRNMACEMCEAHVIEAIRKILSHGEARRIRASHRRNE
                     VTIESEAPLSESLVRECIRSIGETGYPTGAASSEEV"
BASE COUNT          557 a         1092 c          860 g          448 t
ORIGIN      
        1 cgaagggccc gaccgagcca gcaggcgacg acgtcgtggc gaggcaacac cgccgaatca
       61 tccgcggagg gcgctccggg gtgatgggtc gcaggagaac atcctgcgga ctgtccgtcc
      121 gggcatccgg ccggttgcgc tatccccttc agaaacgccg cggatcctgc tttccgcgcc
      181 aaactgtcgg cagaagcgcc tctgtcgacg gtttttcatg tccctgtttc tgaggtacta
      241 cccctatgaa gttcgaaaac gtccgaggct ccatcgtcgc catgatcacg cccttccacg
      301 aagacggaag cgtgaacttc gaagccctca cccatctcat cgagcgccag atcgccggcg
      361 gcacgagcgc cattctcacg ctcggcacga cgggcgaata ctccacgatg acgcacgagg
      421 aggacgccgc cgtcgtgcgc cacacgatcg acacggtcgc gggacgcgtg cccgtcatcg
      481 tcggctcggg ctccaactgc acggccacgc agatcgagat gagccgccgc tacgaggcga
      541 tgggcgccga cgcgctcctc atcatcgccc cgtactacaa caaggccaac gccgagggca
      601 tgtaccgcca cttcgccgac acggccgacg ccgtctccgt gccctgcatc ctctacaacg
      661 tgccgggccg cacgggctgc tccatcccgg tctccgtcgt cgagcgcctc gcgaagcacc
      721 cgaacatctg cggcatcaag gaagcttcgg gcaacatgag ctacgccatg gaggtcgccc
      781 gcttcctcgg ccccgacttc cagatgtggt ccggcaacga cgacatcacg gtccccatca
      841 tgagcgtggg cggctcgggc gtcatcagcg tctacgccaa catcgacccc gcctcctgtg
      901 cgaagatgtg caccgactgg ttcgcgggcc gcaccgccga agcggccacc gagcaggtcc
      961 gccgcctcga cctcatgaac gcgctcttct atgaagtgaa tccgattcct gcgaagactg
     1021 cgctcaacat gatgggcctc aacgccggtc cgatgcgtct gccgctttgc gacatgtcgc
     1081 ccgccaacgc cgcccgcctg cgcgccgagc tcgtcaaggc aggcctcatc aacgaataaa
     1141 gactctggaa agaagcccca tgaacatcat cctcatcgga acgggacgca tggggcgcct
     1201 cctcaaggag tgcgcccttg acgcgggtga cgccgtcgtc ggcgaatacg gccgcgcaaa
     1261 catcgccgaa ctcgccgccg cgcccgcagc cgacgtcgcc atcgacttct ccggccccga
     1321 ggccctgccc gccgtcgccg cctacgtcgc ccgcacgggc accccgctcg tctcgggcac
     1381 cacgggctac acggccgggg aaaaggccgc cgttgtcgcc ctcggcgaac acgcccccgt
     1441 cgtctggagc gccaactact cgctcggcgt cgccgtgctc gcacgcgcgc ttcgcctcgt
     1501 gaccgcagcg ctgcgccccg acttcgacat cgaggtgacc gaaacccacc acaacaggaa
     1561 gaaggacgcc ccgagtggca ccgcgaagct cctcgtcgag gcgctcgatc ccgcgggcga
     1621 actcatgccc gtctacggcc gcgagggcct ctgcggcgaa cgccgtcccg acgaaatcgg
     1681 catccacgcg cttcgcggcg gcaccgttgc aggcacgcat tccgtacact tcttcggccc
     1741 cgacgaagag ctcgagttca cgcaccgcgc cacgagccgc cgcatcttcg tgctcggcgc
     1801 cctcaaggcc gcccgcgcgc tcgtcaggga gcccaagggc ttccattcgc tcgacgacct
     1861 tctcttcaac accaacggaa cccactagat aaatgaacgc ccaggaaatc atcaactaca
     1921 ttgccaccgc tcccaagaag acccccgtca agatctacgt gaacctcagg gagcccgtcg
     1981 acttcgcgtc ctgcggcgcc aaggtcttcg gcgagggcct cagtctcgtc gtcttcggca
     2041 actgggccga ccttgaaccc gtggtcacgg ccaacgccgc cgccatcgcc gacatcatca
     2101 tcgaaaacga ccgccgcaac tccggcgttc cgctccttga catcaggaac atccgcgccc
     2161 gcatcgaacc gggcgccgtg atccgcgaaa aggtcgagat cggcgaaggc gccgtcatca
     2221 tgatgggtgc cgtcatcaac atcggcgccg tcatcggcga aggcaccatg atcgacatgg
     2281 gcgccgtgct cggcggccgc gccatcgtcg gcaaccgatg ccacatcggc gcgggcaccg
     2341 tactcgcagg cgtcgtcgaa cccgcctccg ccacccccgt cgtcatcgag gacgacgtca
     2401 tgatcggcgc caacgccgtc gtgatcgaag gcgtgcgcgt gggccgcgga gccgtcgtcg
     2461 gcgcgggcgc catcgtcatc aaggacgtgc ccgctggcgc cgtcgtggtc ggcaaccccg
     2521 cccgcatcat caagatgaag gacgaaaaga ccgaggccaa gaccgcactc atcgacgcgc
     2581 tgcgcaccct cgactaaacc tcccgagaca gtccgcatcc cgaggggaga ggcgttacga
     2641 cgcccctccc cgattttttg cgtccctctt cgaacgtgat agaatgcgat tcattctcaa
     2701 gtcttttgga acgccaacat gatccgcacc cgaatcgaag tccgcaacat ggcctgcgag
     2761 atgtgcgagg cccacgtgat cgaggccatc cgcaagatcc tctcgcacgg cgaggcgagg
     2821 cgcatcaggg cctcccaccg caggaacgag gtgacgatcg agagcgaggc gccgctctct
     2881 gaaagcctcg tgcgcgaatg catcaggtcc atcggcgaga cgggctatcc cacgggcgcc
     2941 gcctcctccg aagaggt
//
LOCUS       DBMDTI010000253         2948 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7501_length_2948_cov_2.601335, whole genome shotgun sequence.
ACCESSION   DBMDTI010000253 DBMDTI010000000
VERSION     DBMDTI010000253.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7501_length_2948_cov_2.601335"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="ACZPB6_09255"
     CDS             <1..1212
                     /locus_tag="ACZPB6_09255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1184688.1"
                     /translation="GPQMALLLQDRLLSGVAELVPELEEIRHDIHAHPELGFETERTV
                     GLICDYHRKSGIVEIDTELVKGCVIAVIEGSRPGHTIAIRADMDALPMNDGCGKPWAS
                     TVAGRGHCCGHDGHTTWLLGAARWLAAHNDFPGRIVLVHQAAEEAGTGAKALVEAGLI
                     EKYGIEEIYGGHTEPNLPKGVIGFKPGPLQAASDSVYITLRGKGTHGGRPHLGVDPIP
                     VAAQIVTALQTIVSRKVDPIESAVVSICSINCGNFKANNVVQSELTMSGTVRTFLPEI
                     RDLAEENIRAMVTGIAAANGCTVELVYDRMIGSVINGEEQTIAAHEVTKALLGEDNVR
                     IIRPFMSSEDFSEYLNRIPGAIIRVGIRDEEHQVSLHNQQMDFNDEVLPVAVSVVANM
                     AVRRLEALAAR"
     gene            complement(1321..2283)
                     /locus_tag="ACZPB6_09260"
     CDS             complement(1321..2283)
                     /locus_tag="ACZPB6_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR1184689.1"
                     /translation="MSDALLHTISNVLVLVLTAGIGWLAAQTGKLDDTGRITTAKIVN
                     LSIPFFLFNSVTTKFTHDQLVELLGMAWLPFVTVCLNLLISLALIRLGLVRRELSGTF
                     TAAFTGSTVLFVGVPMTTALFGEEGIPYLLVYFFANCVFIWTVGLYAIQTDGVAKTGG
                     ARPKLASMKSVRMLFSPPLLSFIVGIAFVVLSIPIPHFVAATVKSFGSVTSPLALFFI
                     GMTIFKIGVSEMKRMPRELVFVLLSCFVIRPLVMYLVTIPFDLEPIMRKVFVAAAALP
                     VSSVIAVLARTYGGDERFASRAIGVTTLGIIPALPVMLFIVHFV"
     gene            2526..>2948
                     /locus_tag="ACZPB6_09265"
     CDS             2526..>2948
                     /locus_tag="ACZPB6_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR1184690.1"
                     /translation="MLTQLVLITLLIIGAAYFAMSEIALAGSRKVRLERMAEEGDHRA
                     RLVLNLKENPGYFFSVVQVGINAVAILGGVVGEAVFTDVFAAVFAYVVPPEALTPVSF
                     FCSFLLVTILFVMFADLIPKRVAMSCPEKVAVRCISPMV"
BASE COUNT          567 a          925 c          965 g          491 t
ORIGIN      
        1 ggaccccaaa tggctctgct tcttcaagac cgtcttctgt ccggcgtcgc cgaactcgtg
       61 cccgagctcg aggagatccg tcacgacatt catgcacacc ccgagctcgg cttcgagacc
      121 gagcgcaccg tgggcctgat ctgcgactat cacaggaaga gcggcatcgt cgagatcgac
      181 acggagctcg tcaagggctg cgtcatcgcc gtgatcgagg gcagccgtcc cggccacacc
      241 atcgccatcc gtgccgacat ggacgccctg ccgatgaacg acggctgcgg caagccctgg
      301 gcgagcacgg ttgctggccg cggccactgc tgcggccatg acggccacac gacgtggctt
      361 ctcggcgccg cccgctggct cgccgcgcac aacgacttcc ccggccgcat cgtgctcgta
      421 caccaggccg ccgaagaggc cggcacgggt gcgaaggccc tcgtcgaggc gggcctcatc
      481 gagaagtacg gcatcgagga gatctacggc ggccacaccg agcccaatct tcccaagggc
      541 gtgatcggct tcaagccggg tcccctccag gccgcgagcg acagcgtcta catcacgctg
      601 cgcggcaagg gcacgcacgg cggccgtccg cacctcggcg tcgacccgat tcctgtcgcc
      661 gcccagatcg tgacggcgct ccagacgatc gtctcccgca aggtcgaccc gatcgagtcc
      721 gccgtcgtct ccatctgctc gatcaactgc ggcaacttca aggccaacaa cgtcgtgcag
      781 agcgaactga cgatgagcgg cacggtgcgc accttcctgc ccgagatccg cgacctcgcc
      841 gaggagaaca tccgcgcgat ggtgacgggc atcgccgccg ccaacggctg cacggtcgag
      901 ctcgtctacg accgcatgat cggctccgtc atcaacggcg aggagcagac gatcgccgcc
      961 cacgaggtca cgaaggccct gctcggtgag gacaacgtcc gcatcatccg tccgttcatg
     1021 tcgagcgagg acttctccga gtacctcaac cgcatcccag gcgcgatcat ccgcgtgggc
     1081 atccgcgatg aggagcacca ggtgtcgctc cacaatcagc agatggactt caacgacgag
     1141 gtcctccccg tcgccgtgag cgtcgtggcc aacatggccg tcaggcgcct cgaggcgctc
     1201 gccgcccgat gagatcctga acaaggatcg gcattgaaaa ggggtgtccg atctgcatga
     1261 acgggcgccc cttcttcgtg ggtgcgggcg ttgcgcgcag ctttcttttc gagatccgga
     1321 tcagacgaaa tgcacgatga agagcatcac gggcagggcc gggatgatgc cgagtgtggt
     1381 cacgccgatc gctcttgagg cgaagcgctc gtcgccgccg taagtgcgtg cgagaacggc
     1441 gatgaccgac gagacgggca gggcggcggc cgcgacgaag accttgcgca tgatgggctc
     1501 gaggtcgaac gggatcgtga cgaggtacat cacgagcggg cggatcacga agcaggagag
     1561 gagcacgaag acgagttcgc ggggcatgcg cttcatctcg gagacgccga ttttgaagat
     1621 cgtcatgccg atgaagaaga gcgcgagcgg cgaggtgacg gagccgaagc tcttcacggt
     1681 ggccgcgacg aagtgcggga tcggaatcga aaggacgacg aaggcgatgc cgacgatgaa
     1741 ggagagaagc ggcggcgaga agagcattct gacgctcttc atcgaagcga gctttggacg
     1801 cgcgccgccc gtcttcgcga cgccgtcggt ctggatggca tagaggccga cggtccagat
     1861 gaagacgcag ttggcgaaga agtagacgag caggtaggga atgccttctt cgccgaagag
     1921 cgcggtcgtc atgggcacgc ccacgaagag cacggttgag cccgtgaagg ccgcggtgaa
     1981 ggtgcccgag agctccctgc gcacgaggcc gagcctgatg agcgcgagcg agatgagaag
     2041 gttgaggcag accgtcacga acggcagcca ggccatgccg aggagctcga cgagctggtc
     2101 gtgcgtgaac ttcgtcgtga cggaattgaa gaggaagaac ggaatcgaga gattcacgat
     2161 cttcgccgtc gtgatgcggc ccgtgtcgtc gagcttgccc gtttgggcgg caagccagcc
     2221 gatccccgcc gtgaggacga ggacgagcac gttggagatc gtatgaagca gtgcgtcgga
     2281 catttcgtat gtcgtcttgt aggaatccga ggatcaaatg attgtaacag cacgaggatt
     2341 ccttatcctc aggacggaca cctgcgtcga aatcggcggc ggggatcctg atcaggacgt
     2401 cgtgcttcgg gcgggtgcga atttttcccg cccgcaaatc ggtgcgggac gcgggctgcg
     2461 gtacaataag cggccggcgg caacgcccgc ctgtctccgt acgtcatctc tggaaaaccc
     2521 cggtcatgct cacgcagctc gtcctcatca cgcttctcat cataggcgcg gcgtacttcg
     2581 ccatgtctga aatcgccctc gcaggctccc gcaaggtgag gctcgagcgc atggccgaag
     2641 agggcgacca ccgcgccagg ctcgttctca acctcaagga gaatccgggt tacttcttct
     2701 cggtggtgca ggtcggcatc aacgccgtgg ccattctcgg cggcgtggtc ggcgaggcgg
     2761 tcttcacgga cgtcttcgcc gcagtgttcg cctacgtcgt gccgccggag gccctcacgc
     2821 ccgtctcctt cttctgctcg ttcctgctcg tcacgattct cttcgtgatg ttcgcggacc
     2881 tcattccgaa gcgcgtcgcc atgagctgcc ccgaaaaggt ggccgtgcgc tgcatcagtc
     2941 cgatggtg
//
LOCUS       DBMDTI010000254         2947 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7503_length_2947_cov_2.126493, whole genome shotgun sequence.
ACCESSION   DBMDTI010000254 DBMDTI010000000
VERSION     DBMDTI010000254.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7503_length_2947_cov_2.126493"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(74..1510)
                     /locus_tag="ACZPB6_09270"
     CDS             complement(74..1510)
                     /locus_tag="ACZPB6_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR1184691.1"
                     /translation="MLRPSPTAAVLLGLMAALAAGASSAAPSTTAPMGFGDARVLLHE
                     RADIMAADRAEIARAESEAEAAKSLSGPKVEMDVKQVWGTKTVDLGIDTGLGQLSGLG
                     GQIGGVPGQIISSLAQKYNHVDIHFKEDIGGPRAAVTATLPIYTGGLITAQQNALNHK
                     VSETRADRDMRENTLDAELAARYWGVQLARSVAALHRSVFEDQNEELRRAKRFEQKGL
                     IAKVERLAVEVARDNARRTLVSAESDVGVAEAELMRQLRETSLPELSSPLFVLTGDLG
                     TLEQWQNRADAQSPAIRKMNALRAQAGEGISAAEASFKPKVYAFGTKNLIKHYLTLPE
                     PDWMAGVGVTFTLWDNHDRFDKLNAARSVVTKAEAARSEAKNQVATAVEVAFLRVMQA
                     REEFELTGSTVELARENLRLREASFAEGLSNAVDVDTARTKLTGAEVARRAAAYKFVV
                     SWAMLHASAGAMPDFVNSLTRSDLVVVK"
     gene            1730..2383
                     /locus_tag="ACZPB6_09275"
     CDS             1730..2383
                     /locus_tag="ACZPB6_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HIR1184692.1"
                     /translation="MKYKDIYTEAPANPDTLSMLGPIAPLAGKWFGDDGVDTHPQAGG
                     PETEPYVETWDFEVMDPQNNGPQVLYGLRYHVHITKPGELAAFHDQVGYLLYEPETHK
                     IYMTLAIPRGQIAMAEGTAMPGDREIRLHAERGQMVNGICSNPFLEEAFLTKSWDVVF
                     KFHEDGRFSYEQVTKLEIPGVKGEFEHTDANTMRMIEAPRPNPAMIEEGLLNRCPKA"
     gene            2601..>2947
                     /locus_tag="ACZPB6_09280"
     CDS             2601..>2947
                     /locus_tag="ACZPB6_09280"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR1184693.1"
                     /translation="MQLKKIVILTLAALGLAATCTQPLAKTADEVRARCRAEHRPCVG
                     LVLSGGGARGFAHTGVLDVIEELGIKIDVVTGTSMGSMVGGAYAAGYSAAEIRDIVLG
                     VDWDRMMAPRADR"
BASE COUNT          490 a          937 c          982 g          538 t
ORIGIN      
        1 ctgcgcctga gtctgctgag cttgagtctg ttcgatcatt ttgttccagc cggtggaaaa
       61 gcgttgttct gagtcacttc acgaccacga ggtcgctgcg agtaagggag ttgacgaagt
      121 cgggcatggc gcctgcggac gcatggagca tggcccagct cacgacgaac ttgtaggcgg
      181 ccgcgcgtcg ggcgacttcg gcgcccgtca gcttcgtgcg ggccgtgtcg acgtcgacgg
      241 cgttggagag tccttccgcg aagctcgcct cgcgcagtct gaggttttcg cgcgcaagtt
      301 cgacggtcga gccagtgagc tcgaactcct cgcgggcctg catcacgcgc aggaaggcga
      361 cctcgacggc tgtggccacc tggttcttcg cctcgctgcg cgcagcttcg gccttcgtaa
      421 ccacgcttcg ggcggcattg agcttgtcga agcggtcgtg gttgtcccag agcgtgaacg
      481 tgacgcccac gccggccatc cagtcgggct cgggcagtgt cagatagtgc ttgatgaggt
      541 tcttggtgcc gaaggcgtag accttgggct tgaagctcgc ctcggcggcg gagatgcctt
      601 cgcccgcctg agcgcgcagc gcgttcatct tcctgatggc gggtgactgc gcgtcggcgc
      661 ggttctgcca ctgctcaagc gtgcccagat ctcccgtgag gacgaagagc ggcgaggaga
      721 gctcggggag cgaggtttct cgcagctggc gcatcagctc cgcttcggcc acgccgacgt
      781 cggactcggc cgagacgagc gtgcgcctcg cgttgtcgcg tgcaacctcg acggcgaggc
      841 gctcgacttt ggcgatgagg cccttctgct cgaagcgctt cgcgcggcga agctcctcgt
      901 tctgatcctc gaacacgctc ctgtgcaggg ctgcgacgct gcgcgcgagc tgcacgcccc
      961 aatagcgggc cgcaagctcg gcgtcaagcg tgttctcgcg catgtcgcgg tcggcgcgcg
     1021 tctcgctcac cttgtggttg agcgcgttct gctgcgcggt gatgaggccg cccgtgtaga
     1081 tgggaagcgt ggccgtcacg gccgcacgcg gcccgccgat gtcctccttg aagtggatgt
     1141 cgacgtggtt gtacttctgc gccagactcg agatgatctg tccgggcacg ccgccgatct
     1201 ggccgccgag gccggagagc tgtccgagcc ccgtgtcgat gcccaggtcg acggtcttgg
     1261 tgccccagac ctgcttgacg tccatctcga ccttcgggcc cgagagcgac ttggcggcct
     1321 cggcctcgga ctcggcgcgg gcgatttcgg cgcggtcggc ggccatgatg tcggcgcgct
     1381 cgtgcagcag gactcgggcg tcgccgaagc ccatgggggc ggtggtcgac ggtgcggcag
     1441 acgaggcgcc tgcggcgagg gcggccataa ggccgagtaa gaccgcggct gtgggactag
     1501 gacggagcat gttcaggaat ctcgtttgaa aacggaatct gtaatggtaa caccaagtac
     1561 tgcgcccgac ggaaggagcg gagcctctgg cgaaatgaag ggaatgtttt ttcgtacgtt
     1621 tcgtaacggc gggagcacgg ggcccagcgc ggtgttacca tgaaccgagc ttgttcggac
     1681 cgccggagcg cacggtgcgc tgcacgcggt cccttcaaag gaggctgaaa tgaagtacaa
     1741 ggacatctat acggaagcgc ccgccaatcc cgatacgctt tcgatgctcg ggcccattgc
     1801 tcctctggcc ggcaagtggt tcggcgacga cggcgtcgac acccatccgc aggcgggcgg
     1861 ccccgaaacg gagccctacg tcgagacgtg ggacttcgag gtgatggatc cgcagaacaa
     1921 cggcccgcag gtcctctacg gcctgcgcta ccacgtgcac atcaccaagc cgggcgagct
     1981 cgccgccttc catgaccagg tgggctatct gctctacgag cccgagacgc acaagatcta
     2041 catgacgctc gccattccgc gcggccagat cgcgatggcc gagggcacgg ccatgcccgg
     2101 cgaccgcgag atccgtctgc atgccgagcg cggtcagatg gtcaacggca tctgctcgaa
     2161 tcccttcctc gaggaggcct tcctcacgaa gtcctgggac gttgtcttca agttccatga
     2221 ggacggacgc ttcagctacg agcaggtcac gaagctcgag attcccggcg tcaagggcga
     2281 attcgagcac acggatgcca acacgatgcg catgatcgag gccccgcgcc cgaatcccgc
     2341 catgatcgag gaaggactcc tcaaccgctg cccgaaggcc tgacgggcga aaagcctgca
     2401 tttttctcat ttcgacgtcg tccgaaagcg tcagcgagcc gcttcacgcg ctatcatcaa
     2461 gcgtatggag cggcccgatg cattctcgga gaacggacgg ccgcactccg acccaaggag
     2521 cctgcggccg tctttctttg tgccgcaggt ctgcattccg gactcgtccc aattttttcc
     2581 ccgacatcga cgacaagccc atgcaattga agaagattgt gatattgacg cttgcggccc
     2641 tcggccttgc cgcaacctgc acgcagcccc ttgccaagac ggcggacgag gtccgcgcgc
     2701 gatgccgcgc cgagcaccgt ccctgcgtgg gcctcgtgct ctcgggcggc ggcgcccgag
     2761 gcttcgccca tacgggcgtg ctggacgtga tcgaggagct cggcatcaag atcgacgttg
     2821 tgacgggcac gtccatgggc tccatggttg gcggcgccta tgcggcgggc tattcggccg
     2881 ccgaaatccg cgacatcgtg ctcggcgtcg actgggaccg catgatggcg ccccgcgcag
     2941 accgcga
//
LOCUS       DBMDTI010000255         2941 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7523_length_2941_cov_1.725352, whole genome shotgun sequence.
ACCESSION   DBMDTI010000255 DBMDTI010000000
VERSION     DBMDTI010000255.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7523_length_2941_cov_1.725352"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /locus_tag="ACZPB6_09285"
     CDS             <1..1608
                     /locus_tag="ACZPB6_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HIR1184694.1"
                     /translation="RDAWAFVERICGVCTGIHALAAVRSVEDALGIKIPKNANIMRNL
                     VNATLYVHDHLVHFYQLSALDWVDVVSALEADPRETAAIQQKISPSHPLASVGYFRDV
                     QNRLKKFVASGQLGIFKNGYWGHPAMKLPPAVNLLAVTHYLEVLDFQKEIIKIHTILG
                     GKNPHPNYLVGGMACPINMHDTGAQGTMVNEVTLNYMRDIAKRSLEIVENVYLPDIKA
                     IAGFYPEWFKYGAGLSNKNVLCYGEFPEIANDWSDASMQLPRGAIINGNLNEVHDVDL
                     RDLNEIQEFVDHSWYRYPDAGKGLHPWEGITEQAFELSKGSVGDKTKFEWLGADGKYS
                     WIKSPRWKGHMMEVGPLSRLVMGVAKNVPHIKEPAMALLEELNAPLEAVFSTLGRHAA
                     RALECRWAAQKMVQYVDDLTANIRAGDECAANMEKWEPGTWPKEARGVGFCEAPRGAL
                     GHWCVIKDTRIANWQAIVPTTWNASPRSPSGELGAFEQSLIGTPVAVEDQPLEIIRTI
                     HSFDPCLACATHLLSPKGEELCSVKVR"
     gene            1621..2334
                     /gene="cybH"
                     /locus_tag="ACZPB6_09290"
     CDS             1621..2334
                     /gene="cybH"
                     /locus_tag="ACZPB6_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HIR1184695.1"
                     /translation="MKIDPVTFEVDASGGTRPVYVWEAPVRVWHWLMAVAMFAMIITG
                     YLIGRPLVSNLGDTWITYDFGYVRMVHFAAGLFFTALFIYRLFWVAVGNRYARMIFFP
                     PLLSMKWWKGVFAQVAYYLFMRKSAPEYAGHNPLAQLAMFAMFVLGSFVIIITGLALY
                     AQSWGWDTSWMAWFGWVFVLFGDAQAVRTVHHVMMYVFILFSIAHIYMSFREDVMGGA
                     TTLSSMSTGLRMFKEDHHG"
BASE COUNT          544 a          946 c          880 g          571 t
ORIGIN      
        1 cgcgacgcct gggccttcgt cgaacgcatc tgcggcgtct gcacgggcat tcacgccctt
       61 gcggccgtgc gcagcgtcga ggacgccctc gggatcaaga tcccgaagaa cgccaacatc
      121 atgcgcaacc tggtcaatgc cacgctctac gtgcacgacc atctggtgca cttctaccag
      181 ctctccgcgc tcgactgggt agacgtggtc tcggcgcttg aggccgatcc ccgcgaaacc
      241 gcggcgatcc agcagaagat ctccccgagt catccgcttg cctccgtggg ctacttccgt
      301 gacgtccaga accgcctgaa gaagttcgtc gcgagcggcc agctcggcat cttcaagaac
      361 ggctactggg gccatccggc catgaagctg ccgcccgccg tgaaccttct tgccgtcacg
      421 cactatcttg aagtgctcga cttccagaag gagatcatca agatccacac gatccttggc
      481 ggcaagaatc cgcatccgaa ctatctggtc ggcggcatgg cctgcccgat caacatgcac
      541 gatacgggcg cccagggcac gatggtcaac gaggtgacgc tcaactacat gcgcgacatt
      601 gcgaagcgct cgctcgagat cgtcgagaac gtctatctgc cggacatcaa ggccatcgcg
      661 ggcttctatc ccgaatggtt caagtacggc gcgggcctct ccaacaagaa tgtcctctgc
      721 tacggcgaat ttcccgaaat cgccaacgac tggtccgatg cctcgatgca gctgccgcgg
      781 ggcgcgatca tcaacggcaa cctcaacgag gtgcacgacg tcgacctgcg cgacctcaac
      841 gagatccagg aattcgtgga ccactcgtgg taccgctatc cggacgccgg caagggcctg
      901 catccgtggg aaggcatcac cgagcaggcc ttcgagctct cgaagggctc cgtgggcgac
      961 aagacgaagt tcgaatggct cggcgccgac ggcaagtact cctggatcaa gagcccgcgc
     1021 tggaagggcc acatgatgga agtcggtccg ctctcgcgcc tcgtcatggg cgtcgccaag
     1081 aacgtgccgc acatcaagga gcccgccatg gcgctcctcg aggagctcaa cgcgccgctc
     1141 gaggccgtct tctcgacgct cggccgtcac gcagcccgcg cgctcgagtg ccgttgggcc
     1201 gcccagaaga tggtccagta cgtcgacgac ctgacggcca acatccgtgc gggcgacgaa
     1261 tgcgccgcca acatggagaa gtgggagccc ggcacctggc ccaaggaagc ccgcggcgtg
     1321 ggcttctgcg aggccccgcg cggcgccctc ggccactggt gcgtcatcaa ggacacgcgc
     1381 atcgccaact ggcaggccat cgtgccgacg acgtggaacg cgtcgccgcg ctctccctcg
     1441 ggcgagctcg gcgccttcga gcagagcctc atcggcaccc cggtcgccgt cgaggaccag
     1501 ccgctcgaaa tcatccgcac gatccacagc ttcgacccgt gcctggcctg cgccactcat
     1561 ctcctgagcc cgaagggcga ggaactctgc agcgtcaagg tgcgctgatg ggaggccgct
     1621 atgaaaatcg atcccgtcac ttttgaagtc gatgcgagcg gaggcacccg gcccgtatac
     1681 gtctgggagg ccccggtccg agtctggcac tggctgatgg ccgtcgcgat gttcgcgatg
     1741 atcatcaccg gctacctgat cggccgaccg cttgtctcca acctcgggga cacgtggatc
     1801 acctacgact tcggctacgt gcgcatggtg cacttcgccg cggggctgtt cttcacggcg
     1861 ctcttcatct accgcctctt ctgggtggcg gtgggcaacc gctacgcgag gatgatcttc
     1921 ttcccgccgc tgttgtcgat gaagtggtgg aagggcgtgt tcgcccaggt cgcctactac
     1981 ctcttcatga ggaagtccgc acctgaatac gccggccaca acccgcttgc gcagctcgcc
     2041 atgttcgcga tgttcgtgct cggctccttc gtgatcatca tcacgggcct cgcgctctac
     2101 gcgcagtcct ggggctggga cacgagctgg atggcctggt tcggctgggt gttcgtgctc
     2161 ttcggagacg cccaggcggt ccgcacggtg caccacgtga tgatgtacgt gttcatcctc
     2221 ttcagcatcg cccatatcta catgagcttc cgcgaagacg tcatgggcgg cgccaccacg
     2281 ctctcctcga tgagcaccgg cctgcgcatg ttcaaggagg atcatcacgg ttgagcctga
     2341 ttgtctcagg tgactgaagc cgcaaaacaa aggggcccgt tcaggcagca atgccgggac
     2401 gggcctcttt ttgttgtctt gtttcttctt ttctggtcct gaagtgggtt gggatgctga
     2461 ggacgcagtc gggcgtccga ggatgcggag ccgggccagc ccgacacttt ttcccctgcg
     2521 ggcggccgct ccatgggtcc gcattatgct cctaaaaacc gactgagcgg tatttgttgt
     2581 tgagggaaag tgtcatgagg gaaattccga cctgtggtac agattttgca taagccttga
     2641 tgctcaaggg aagggtaatc gttattggtg aaaaccctaa gggattgtcc taggttttta
     2701 gctgatataa aaccgaccgg acggaatgta ttggtgtatg atgcgtaccg tcgtgttctc
     2761 ccacactcca agcagttcct cagaggaccg tcctcagggg aactcatctt aaggaccagc
     2821 caaaatgttc aagaagactc ttctctccgc cgccgctctt ctcgcggccg gcacgctttc
     2881 tgcttccgtc ttcgccgctc cggtgaccgc cgagggcacg ggcgttggca agcacggcga
     2941 c
//
LOCUS       DBMDTI010000256         2939 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7529_length_2939_cov_2.784355, whole genome shotgun sequence.
ACCESSION   DBMDTI010000256 DBMDTI010000000
VERSION     DBMDTI010000256.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7529_length_2939_cov_2.784355"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="ACZPB6_09295"
     CDS             <1..955
                     /locus_tag="ACZPB6_09295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1184696.1"
                     /translation="RQHIANLERTGIVGQMSTIIAHELKQPLGAITNYGNGLLRRLGR
                     GDVEREKLELALREIVLQAERASKIVERVRSYAKHDYPPRKVSDLSIIIENAIQTFRR
                     SRTTDAELTVRMHPHSMAEVDGWEIELVVLNLLKNAADAISGIPDPKIEVCLYPSDEH
                     TWILTVGDNGPYISDEQLSRFFKPLQTSKGEAGMGLGLSIVANIAERHAGNITVARNG
                     SRGVKFTMTIPSQMKDVSVIADMGPETVSIYESGGNGRVAREVVERRGEEQADESPQL
                     ERSLPATVHTGTLNDAVHLMQDGDMHRTGRPRPPGIPGSKG"
     gene            1039..1431
                     /locus_tag="ACZPB6_09300"
     CDS             1039..1431
                     /locus_tag="ACZPB6_09300"
                     /inference="COORDINATES: protein motif:HMM:NF025745.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4418 family protein"
                     /protein_id="HIR1184697.1"
                     /translation="MKRPAFLSGFLMMLVGLGLTIAVMFVLPFHKDAAAPCFYLVRMT
                     AVLGFASALAGVMLQFSSAGLAAGIQLMNMLLGIVITLAAAFVIGPCAVENAPCAENT
                     APVVMIWGAVIAFLSAADALRLSKRRSE"
     gene            1460..1570
                     /locus_tag="ACZPB6_09305"
                     /pseudo
     CDS             1460..1570
                     /locus_tag="ACZPB6_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1630..1920
                     /locus_tag="ACZPB6_09310"
     CDS             1630..1920
                     /locus_tag="ACZPB6_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184698.1"
                     /translation="MVRIQDGTPPERLRRIIGRFSSNNHGCILFQRMFIDKDRENALS
                     LAKSAYNREIGRPTKTCAPCASATATGSSPTPRCSMRRRSALERHERDVRAL"
     gene            complement(2059..2766)
                     /locus_tag="ACZPB6_09315"
     CDS             complement(2059..2766)
                     /locus_tag="ACZPB6_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1184699.1"
                     /translation="MSNLECKHRLYKKVGLIGKAFSHEVRLELIELLSQCPQTVEKLS
                     EMLDEDYKSISAHLRVLYRAGLVTCTRNGRFQRYGLASPKVTALAVMIRETAEQSIAE
                     LPCLEAESGIPNASLGIEDAVHYASEGKLILLDVRPAEEFAAGHLPHAINMPIEMLGK
                     RMHELPRGMALAAYCRGPYCFLAHEAAAILEASGRRLQVIPSGVMEWSSRGAELESAS
                     PEESQKKPPRRGIRTQA"
BASE COUNT          514 a          953 c          937 g          535 t
ORIGIN      
        1 gaggcagcac atcgcgaacc tcgagcgcac gggcatcgtg ggccagatgt cgaccatcat
       61 cgcgcacgag ctcaagcagc ctctgggcgc catcaccaac tacggcaacg gccttctgag
      121 gcgcctcggg cgcggtgacg tcgagcgcga gaagcttgag ctcgccctga gggagatcgt
      181 cctgcaggcc gaacgcgcga gcaagatcgt cgagcgcgtg cgctcctacg ccaagcacga
      241 ctatccgccg cgcaaggtct ccgacctctc gatcatcatc gagaacgcca tccagacctt
      301 caggcgctcg cgcacgaccg acgctgagct gacggtgcgc atgcatccgc acagcatggc
      361 cgaggtcgac ggctgggaga tcgagctcgt ggtgctcaac ctcctcaaga acgccgccga
      421 cgccatttcg ggcattcccg atccgaagat cgaggtctgc ctctatccga gcgacgagca
      481 cacctggatc ctgacggtgg gcgacaacgg tccctacatc tcggacgagc agctctcgcg
      541 cttcttcaag ccgctgcaga caagcaaggg cgaggccggc atgggcctcg ggctctcgat
      601 cgtcgccaac atcgccgagc gccatgcggg caacatcacg gttgcacgca acggctcgcg
      661 gggcgtgaag ttcacgatga cgatcccctc ccagatgaag gacgtctccg tcatcgccga
      721 catgggcccc gagaccgtca gcatctacga gtcgggcggc aacggtcgcg tggcccgcga
      781 ggtggtcgag aggcgcggcg aggagcaggc cgacgagtcg ccgcagctcg agagaagcct
      841 ccccgccacg gtgcacacgg gcaccctcaa cgatgcggtc cacctgatgc aggacggtga
      901 catgcaccgc acgggccgcc cgcgcccgcc cggtatcccc ggcagcaagg ggtgatcgag
      961 ggcttgagcc gagggggcgg gacgggtaga attcgccttc ccgcatcccg caccgaattt
     1021 cctggagtct tattcaacat gaagcgtccc gcctttctct ccggctttct gatgatgctc
     1081 gtgggcctcg gcctcacgat cgccgtcatg ttcgtcctgc cgtttcacaa ggacgccgcc
     1141 gcgccgtgct tctacctcgt gcgcatgacg gccgtccttg gcttcgcgag cgcccttgcg
     1201 ggcgtcatgc tgcagttctc gtccgcaggc cttgcggcgg gcatccagct catgaacatg
     1261 ctgctcggca tcgtcatcac gctcgccgcc gccttcgtca tcggcccgtg cgccgtcgag
     1321 aacgcgccct gcgccgagaa caccgcgccc gtcgtcatga tctggggcgc cgtcatcgcc
     1381 ttcctctcgg ccgccgacgc ccttcgcctt tcgaagaggc gttcggaata agcatccgga
     1441 cattctcttt gaagtcgcgc tgaggctttt cgcccgcaac gggtatgcag gcgtttcgat
     1501 gcgcgacgtg gcgcttgagg tcggcttcac gcaggcggcg atctactacc attttttcca
     1561 acaagatcga cttctacagg catgcggtcg accatgcgat caagcaggtg acggacagca
     1621 tcgaggcaga tggtgcgcat tcaggacggc acgcctcccg agaggctgcg ccgcatcatc
     1681 ggccgctttt cctccaacaa ccacggctgc attctctttc agcgcatgtt catcgacaag
     1741 gatcgcgaga atgcgctcag cctcgcgaag tccgcctaca accgcgagat cggcaggccg
     1801 acgaaaacct gcgcgccgtg cgcgagcgct accgccacgg gctcgtctcc cacaccgagg
     1861 tgctcgatgc ggagacgctc ggctctcgag cgacatgaac gcgatgtacg cgcactatga
     1921 cgccgtgctc gcgggactga ggctcaggca cgcgatgagc gaactctgaa gttcacgctc
     1981 tcccaaagga aaaacccgcc gtctttgttc aagcggcggg atttcgtcat tatgtccttc
     2041 gtcagggatc cgtcggactc aggcctgcgt tctgatgccg cgtcgcggcg gctttttctg
     2101 ggattcctcg gggctggcgg actcgagttc ggcgcccctg ctcgaccact ccatgacgcc
     2161 cgaggggatc acctgcaggc gtcggcccga agcctcgaga atggctgcgg cctcgtgcgc
     2221 gaggaagcag tagggaccgc ggcagtaggc ggcgagcgcc atgcctcggg gcagctcgtg
     2281 catgcgcttg ccgagcatct cgatgggcat gttgatggcg tggggaaggt gtcccgccgc
     2341 aaattcctcc gcggggcgaa cgtcgagcag gatgagcttg ccttcggagg catagtggac
     2401 ggcgtcctcg atgccgagcg aggcgttggg aatgccgctt tcggcctcaa gacagggcag
     2461 ttcggcgatg ctctgctcgg ccgtctcgcg gatcatgacg gcaagcgccg tcaccttggg
     2521 gctcgcaagc ccatagcgct ggaagcggcc gttgcgggtg caggtcacga gccccgcgcg
     2581 atagaggacg cgaaggtgcg cgctgatgct cttgtagtcc tcgtcgagca tctcggaaag
     2641 cttttcgacg gtctgcggac actgggagag gagctcgatg agctcgagcc ggacttcatg
     2701 actgaaggcc ttgccgatca ggccgacctt cttgtagagg cggtgcttgc attcgaggtt
     2761 gctcaagggg tctctccgtt tgtgcttgtg tcgttccgct cgtcccgcgg ggttgtcgga
     2821 cgagcgggct tgtttgagaa catgcgacat tttacggcaa aatgcgcgga cgttcggatt
     2881 ttttcacgaa cgacgcgcgt gaagacgaaa aagcccggag gcctttcggc gtccgggca
//
LOCUS       DBMDTI010000257         2916 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7602_length_2916_cov_2.345293, whole genome shotgun sequence.
ACCESSION   DBMDTI010000257 DBMDTI010000000
VERSION     DBMDTI010000257.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7602_length_2916_cov_2.345293"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(23..99)
                     /locus_tag="ACZPB6_09320"
     tRNA            complement(23..99)
                     /locus_tag="ACZPB6_09320"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Val,seq:gac)
     gene            complement(290..2401)
                     /locus_tag="ACZPB6_09325"
     CDS             complement(290..2401)
                     /locus_tag="ACZPB6_09325"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR1184700.1"
                     /translation="MSETPALVVDVSLARSMVEPLYRDLTLPTGENVMQHADGIASIL
                     REVRDDPELISAAYLFCVPSVIQNSGEWIEKSFGPAVNGLVQELGQVNELSRRARSEN
                     EEANTRHQVEAMRRMFLSMCKDLRVVLLKLASRLQTLRWFAASKREGAEKFGTETLDL
                     YAPLANRLGIWQLKWELEDLSLRFTRPSEYREIAQQLDESREQRIEFMQSAVLRIRGL
                     LEENGIKAEVSGRPKHIYSIWKKMQRKHLRFDQLFDVRAVRIIVDTVERCYQVLSIVH
                     ENFPVISKEYDDYIAKPKPNGYQSLHTVVTDKAGRPLEIQIRTRAMHEFAELGVAAHW
                     RYKEAGNSNGATSAEEQRVAWLRQLLAWGSDMQNQGQPEGEAVLDDHIYALTPQGRVV
                     ELPRGGTPVDFAYAVHTQLGHRTKGAKVDGVLVPLNTKLETGQTVEIIAAKVGQPSRD
                     WLSAEKGFAASPRTRNKVRQWFNAQQTAELTAQGREKIDKELARLGKTAVKLEDLAKR
                     LGYEGVDELCLAYGKEEITQTALATAVQPPKPVAPEPDEVLIRQSRNTGKSDVLVVGV
                     DSLLTQLARCCHPVPPDEIVGYVTRGRGVTIHRADCPNIRNMSEQDHGRLIEVSWGRG
                     GDGVFPAEVFVVATDRPGLIRDISEIFMKAKQNLVGMNSQKVKTDVHMRFTVEIPSAS
                     SLTSTLTTIRELQGVLSARRC"
     gene            complement(2594..>2916)
                     /locus_tag="ACZPB6_09330"
     CDS             complement(2594..>2916)
                     /locus_tag="ACZPB6_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184701.1"
                     /translation="EQVGGHAAAAGGLAQALPSGVLVLDQGRRHARGRVRAGDLEVRQ
                     TVSAGGASSSIGHEDLTRGEIFGDAALKVRLRDSVAGLHVGGSTGPDGSRSWTGGVSF
                     DYQF"
BASE COUNT          510 a          916 c          942 g          548 t
ORIGIN      
        1 aacaaaatcc caaataaaat tctggtaggc acgattggat tcgaaccaac gacccccacc
       61 atgtcaaggt gatgctctaa ccaactgagc tacgtgcctg ccgtgtgaga acgaaattat
      121 gcctgagatt tttgaaaaag caagaccatc gaaaaataat ttttcgacgc tccgcaccta
      181 aaaactcgaa agaccctgtc gcacaacaaa aaaggcgaga agccgaaatt tctcgcctac
      241 tgtttttacg gtattttatt tgtaacccag cctcaggagg cctgcaaaat cagcagcggc
      301 gtgccgacag gacgccctgg agctcgcgaa tggtcgtgag cgtggacgtc aggctcgaag
      361 ccgagggaat ctcgaccgtg aagcgcatgt ggacgtcggt cttcaccttc tgggaattca
      421 tgcccacgag gttctgcttg gccttcatga agatttccga gatgtccctg atgaggccgg
      481 gacggtcggt cgcgacgacg aagacctcgg cggggaagac gccgtcgcct cccctgcccc
      541 agctcacttc gatgaggcgg ccgtggtcct gctcggacat gttgcggatg ttcgggcagt
      601 cggcgcggtg gatcgtcacg ccgcggccgc gggtgacgta gccgacgatc tcgtcgggcg
      661 gcacgggatg gcagcagcgg gcgagctgcg tgagaagcga atcgacgccc acgacgagca
      721 cgtcgctctt gcccgtgttg cgggactggc ggatgaggac ctcgtcgggc tcgggcgcga
      781 cgggcttggg cggctgcaca gcggtggcga gcgccgtctg cgtgatctcc tccttgccgt
      841 atgcaaggca gagctcgtcg acgccctcat agccgaggcg cttggcgaga tcctcgagct
      901 tgaccgcagt cttgccgagg cgcgcaagct ccttgtcgat cttctcgcgg ccctgggcgg
      961 tgagctccgc agtctgctgg gcgttgaacc actggcggac cttgttgcgg gtgcgcggac
     1021 tggccgcaaa gcccttctcg gcggagagcc agtcgcggga cggctgtccc accttggcgg
     1081 cgatgatctc gacggtctgg cccgtctcga gcttggtgtt gagcggcacg agcacgccgt
     1141 cgacctttgc gcccttggtg cggtggccga gctgcgtgtg cacggcatag gcgaagtcga
     1201 cgggcgtgcc cccgcggggc agctcgacca cgcggccctg cggcgtgagc gcatagatgt
     1261 ggtcgtcgag cacggcctcg ccctcgggct ggccctggtt ctgcatgtcg ctgccccagg
     1321 cgagcagctg gcgaagccag gcgacgcgct gctcctcggc gctcgtcgcg ccgttggagt
     1381 tgcctgcctc cttgtagcgc cagtgcgcgg ccacgccgag ctctgcgaac tcatgcatgg
     1441 cgcgggtgcg gatctggatc tcgagcggac ggcccgcctt gtcggtcacg accgtgtgaa
     1501 gcgactggta gccgttgggc ttgggcttgg cgatgtagtc gtcgtactcc ttcgaaatca
     1561 cggggaagtt ctcgtgcacg atggagagca cctggtagca gcgctcgacc gtgtcgacaa
     1621 tgatgcgcac ggcgcgcacg tcgaagagct gatcaaagcg caggtgcttg cgctgcatct
     1681 tcttccagat cgaatagatg tgcttcggac gtcccgagac ttcggccttg atgccgttct
     1741 cctcaagaag tccgcggatg cgcagcacgg cgctctgcat gaactcgatg cgctgctcgc
     1801 gcgattcgtc gagttgctgt gcgatctcac ggtattcgga cgggcgcgtg aaacgcagcg
     1861 acaggtcctc gagctcccac ttgagctgcc agatgccgag gcggttggcc agcggtgcgt
     1921 aaaggtcgag cgtctcggtg ccgaacttct cggcgccctc gcgcttcgag gcggcgaacc
     1981 agcgcagcgt ttgcaggcgg ctcgcaagct tgagaaggac gacgcgcagg tccttgcaca
     2041 tcgaaaggaa catgcggcgc atggcctcca cctgatgacg cgtgttcgcc tcctcgtttt
     2101 cggaacgggc gcggcgggaa agttcgttca cttgtccgag ctcctgcacg agcccgttga
     2161 cggcgggacc gaagctcttt tcgatccatt cgccggaatt ctggatcacg gaaggcacgc
     2221 agaaaagata ggctgcgctg atgagttccg gatcgtccct gacctctctg agaatgcttg
     2281 cgatgccgtc ggcatgctgc atgacgttct caccggtcgg aagggtaagg tcgcgataga
     2341 gcggctcgac catgctgcgt gcaagcgaga cgtccacgac cagggcaggc gtttccgaca
     2401 tttctgtttt atctcctcca ggcgcctccg caggcctctt tcccagcagc ggagggcttc
     2461 aacagtcatg caggcggacg tcccgcggac ttcccgcagg gcgcacgcct gcatcttcca
     2521 ttttagagga tcatctgtcg gataggcaga cccgcgacgc ccgacgcctt ccgacgacgg
     2581 gcgcagaccc cgcctagaac tggtagtcga acgacacgcc gcctgtccac gaacgcgagc
     2641 cgtcgggccc agtggagccg ccgacatgca gccccgccac gctgtcgcgc agacgcacct
     2701 tgagggcggc gtcgccgaag atctcgccgc gcgtcaggtc ctcgtgcccg atgcttgagg
     2761 aggcgcctcc cgcgctcacc gtctgcctga cctcaaggtc gcccgcgcgc acgcgtcccc
     2821 gagcgtgacg tcggccttga tccaggacga ggacgccgga cggaagagcc tgtgccaacc
     2881 cgcctgcagc tgcagcgtgc ccacccacct gctctc
//
LOCUS       DBMDTI010000258         2913 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7617_length_2913_cov_2.394737, whole genome shotgun sequence.
ACCESSION   DBMDTI010000258 DBMDTI010000000
VERSION     DBMDTI010000258.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7617_length_2913_cov_2.394737"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>2913
                     /locus_tag="ACZPB6_09335"
     CDS             <1..>2913
                     /locus_tag="ACZPB6_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012403.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HIR1184702.1"
                     /translation="IAHGSAYSPYEVAIDLKPLPSEDVTRLVDAVAERPALLAKLLEG
                     ELDPEVGEIATELGISLFPQSWREFRMSCSCPDDAVPCKHIAGVYYGMVKTIDADPMW
                     VFHFRGVDLPGLLRDRGIDLDKSVSLFEPDPLVWLALADPKGEAEEGDGEALPLLEEI
                     EGGYLKRLASLLPASVEGGKALSRYRYEKLVAPVMSRSRSHEGQGHDVDELWNKFQSA
                     FSGGRVLPELLWADGRFMMSLRIPRGRSLDASMMDRGRLIISLSELDGRTPASAPGLE
                     PWSRVAKAAVMLLRQGAAVPVLVHLKTEGRDKVAAMWMPAMQAVGVRRFVEETGAILS
                     KDVLGIFKKSGCPLAQSTRTGRLFTLLSVLMTEYVEFSARVPSTFAGDPLYALMARPL
                     SSALDYGIAQADITLVRKFLKPFSLAFMQLSWVPVMTVRTAKNGNVTVNLGVLPRNAG
                     PKARPVLYRDVLKEEKFEADRLAILSVFESLAVYCNELRAVLDSKGKPATLPKDGLRD
                     FLFDAVPSLELLGARVMLPKSLSNLLKPKLSVSMSGSTGKGMITKESVGSFDWKVSLG
                     ERVLTKEEFEAVMAHVGEVIPFNDEFVYLDPEVLRKLKAKVDFMESAGYLDMMKAVYT
                     GELDDGTAVSVPDDLIERTREFGRVDSIPLPSGLNAVLRPYQERGYSWLMRNLMLGLG
                     ALIADDMGLGKTLQVITTLLAMKERGEFAKEKAIAIVPATLMTNWMREIERFAPGLTA
                     SVYHGSARQLAPVEERPDVTITTYGTFKRDAAVLGAETWRLMVLDEAQAVKNTGSGIT
                     QAVRDFPARQVIAMSGTPVENRLMEYWSLLSIVQPGILGTQDEFMKSFAKPIETDRNE
                     RALEAFRLVTAPFMLRRLKTDKSIISDLPDRVVCDRYVDLTPEQAALYKTTLDYWMKK
                     LEGLGQEDGMKRSSMLLSLITALKQICNSPSLYEKRHPRLPDSGKADSLFELVEEC"
BASE COUNT          527 a          908 c          920 g          558 t
ORIGIN      
        1 gattgcgcat ggcagcgcct actcgcccta cgaggttgcg attgacttga agcccttgcc
       61 gtccgaggac gtcacgaggc tcgtcgatgc tgttgccgag aggcccgcgc tgcttgcgaa
      121 gcttctcgag ggcgagctcg accccgaggt cggcgagatt gcaacggagc tcggcatcag
      181 cctcttcccg cagtcgtgga gggagttccg catgtcctgc tcatgtcccg acgatgcggt
      241 tccctgcaag catattgcgg gtgtctacta cggaatggtg aagacgatcg acgcggaccc
      301 gatgtgggtc ttccacttca ggggcgttga tctgcccgga cttctgcgcg atcgcggtat
      361 cgatcttgac aagtccgtct cgctgttcga gccggatcct cttgtctggc ttgcgctcgc
      421 cgatcccaag ggggaggcag aagaggggga cggcgaagcg ctcccgctgc tcgaggaaat
      481 tgagggcgga tatctcaagc gcctcgcgtc gcttctgccc gcatcggtcg agggcgggaa
      541 ggcgctttca cgctaccgct atgaaaagct cgtcgccccc gtgatgagca ggtcccgttc
      601 ccacgaaggt caggggcatg acgttgacga gctctggaac aagttccagt ccgccttctc
      661 aggtggacgc gtgctgccgg aacttctctg ggcggacggt cgcttcatga tgtcgcttcg
      721 cataccgcgc ggccgcagtc tcgatgcgtc gatgatggac cggggccgcc tcatcatctc
      781 gctctccgag ctcgacgggc gcacgcccgc gtccgccccc gggctcgaac cctggagccg
      841 agtcgcgaag gccgccgtca tgctcctgag gcagggggct gcggtccccg tgctcgtgca
      901 cctcaagacg gaaggtcgcg acaaggtggc ggcgatgtgg atgcctgcga tgcaggcggt
      961 tggcgtgcgc aggttcgtcg aggagacggg cgcgattctc tcgaaggatg ttctcggcat
     1021 attcaagaag agcgggtgcc cgctcgcaca atccacgcgg accggcaggc tctttacgct
     1081 tctctccgtg ctgatgaccg aatatgtcga attctcggcg cgcgtgcctt cgacctttgc
     1141 gggcgatccg ctttacgccc tgatggccag gcccctgagc tcggcgctcg attacggcat
     1201 cgcccaagcc gacattacgc tcgtgcgcaa gttcctcaag ccgttctcgc tcgccttcat
     1261 gcagctctcc tgggttcccg tcatgacggt ccgcactgcg aagaacggca acgtgacggt
     1321 taatctcggc gtccttcccc gcaacgccgg tcccaaggcg cgtcccgtgc tctacaggga
     1381 cgtgctcaag gaggagaagt tcgaggccga ccgtctggcg atcctttccg tcttcgaatc
     1441 cctcgccgtc tactgcaacg agctcagggc agtgctcgac tcaaagggca agccagcgac
     1501 gctcccgaag gacggactgc gcgactttct ctttgacgcc gtgccttcgc tcgagttgct
     1561 cggcgcccgc gtcatgctcc ccaagagcct cagcaatctc ctcaagccga agctgtccgt
     1621 ctccatgtcg ggcagcacgg gcaagggtat gatcacgaag gagtccgtcg gaagctttga
     1681 ctggaaggtg tctctgggag agcgggtgct caccaaggaa gaatttgagg ctgtcatggc
     1741 tcatgtggga gaggtcattc ccttcaatga cgagttcgtg tatctcgatc ccgaagtgct
     1801 cagaaagctg aaggcgaagg tcgacttcat ggagagtgcg ggatatctcg acatgatgaa
     1861 ggccgtctat acgggcgaac tcgacgacgg cacggccgtc tcggttcccg acgacctgat
     1921 tgagcgcacc cgtgagttcg ggcgcgtgga ttcgattccg ctgccttcgg gcctcaacgc
     1981 ggtgctgcgc ccctatcagg aaagaggcta ttcgtggctc atgagaaatc tgatgctcgg
     2041 actcggcgcc ctcattgccg acgacatggg cctcgggaag acgctgcagg tcatcacgac
     2101 gcttctagcc atgaaggagc gaggcgagtt tgcgaaggaa aaggcgatcg cgatcgtgcc
     2161 cgccaccctg atgacaaact ggatgcgtga gatcgagcgc ttcgcgcccg gcctcactgc
     2221 atccgtctac cacggctcgg ccaggcagct tgcgcccgtc gaggagcggc ccgacgtgac
     2281 gatcacgacc tacggcacct tcaagcgcga tgcggcggtc cttggggccg agacttggag
     2341 gctgatggtg ctcgacgagg cgcaggccgt caagaacacg ggctccggca tcacgcaggc
     2401 ggttcgtgac ttccccgcca gacaggtgat cgccatgagc gggacgcccg tggagaacag
     2461 gctgatggag tactggtcgc tcctgtcgat cgtgcagccc ggcatcctcg gcacccagga
     2521 cgaattcatg aagtccttcg ccaagcccat cgagacggac aggaacgagc gtgcgctcga
     2581 ggccttccgc ctggtgaccg ctcccttcat gctcaggcgc ctcaagaccg acaagtcgat
     2641 catctcggat ctgcccgaca gggtggtctg cgaccgctac gtcgatctca cgcccgagca
     2701 ggccgcactc tacaagacga cgctcgacta ctggatgaag aagcttgagg ggctcggaca
     2761 ggaggacggc atgaagaggt cgagcatgct gctgagcctc atcacggccc tcaagcagat
     2821 ctgcaactcc ccgtcccttt atgaaaagag gcacccgcgg cttcccgaca gcggcaaggc
     2881 cgactcgctc ttcgagcttg tcgaggagtg cag
//
LOCUS       DBMDTI010000259         2907 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7634_length_2907_cov_3.923022, whole genome shotgun sequence.
ACCESSION   DBMDTI010000259 DBMDTI010000000
VERSION     DBMDTI010000259.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7634_length_2907_cov_3.923022"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /gene="ettA"
                     /locus_tag="ACZPB6_09340"
     CDS             <1..1038
                     /gene="ettA"
                     /locus_tag="ACZPB6_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR1184703.1"
                     /translation="TVVAVTHDRYFLDNAAEWILELDRGEGIPWKGNYSSWLDQKEKR
                     LEIEKRQEDARMKAIKHELEWVRQNPKGRQAKSKARIARFEELSSVEYQQRNETNEIF
                     IPVAERLGNDVIEFNHVSKGFGDRLLIDDLSFRIPAGAIVGVIGPNGAGKSTLFKMIT
                     GREQPDSGEIKIGTTVKLAAVDQMRDSLPNDKTAFDAVADGQDILQVGKFSMSSRAYL
                     GRFNFKGGDQQKLVGSLSGGERGRLHLAKTLLAGGNVLLLDEPSNDLDVETLRALEEA
                     LLEFAGCALVTSHDRWFLDRIATHILAFEGDSRVVFFDGNYNEYEADKRRRLGEEACQ
                     PKRLRFKPLKH"
     gene            1077..2714
                     /gene="mnmC1"
                     /locus_tag="ACZPB6_09345"
     CDS             1077..2714
                     /gene="mnmC1"
                     /locus_tag="ACZPB6_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC1"
                     /protein_id="HIR1184704.1"
                     /translation="MTVHLLTSAKKPLLDFVRQRLLPLEPCEVRTVLVPVAQETAEDV
                     AQALGLVPGDSGFASMPGLHVVPCGGTRLVLVRAETAAAALRETTTVPDVLVSMPEDI
                     NGGFRRLMGARTRLVTTAPLERAPGFLEPDGESWRLRSARKAPEEQQEQQGGGSSASE
                     RVLIVGAGLAGAMTAWELAQRGSRAVVVDAGPVPGSGASALHAGLIHPHWQASDSPLF
                     QLTRAGFEAMTEVLRDFPDAFIPEGVVDAASSEEEYEKWREAAAYGRPMHLPGDFASL
                     LTREEASERAGLALSRGGWLYPKAGFVHAGRLARRMLEAAQAQVLTNMPVSLRRREGL
                     WEAVSAQGVVVARAPKAVVCAALATPCVLGLARGTMGLSPLYGRISLLRETDLPELRC
                     ALTGDGYVARTEGFCAVGATYEPGEAPDVAVQEAHEHNLSTFDKLTGRRPDVLAAGFY
                     EGVRAVPADRMPLAGRGWTVAELEGLAFRGVPEARSIPRAPGLWICAGFGSRGLTWGL
                     ACARHVAADVTGDVQALPGSLAAKLDPARFLPKLLAG"
BASE COUNT          506 a          928 c          966 g          507 t
ORIGIN      
        1 accgtcgtcg ccgtgaccca tgaccgctac ttcctcgaca acgcggccga atggattctc
       61 gaactcgacc gcggcgaagg cattccctgg aagggcaact actcctcctg gcttgaccag
      121 aaggaaaagc gccttgagat cgaaaagcgt caggaagacg cccgcatgaa ggccatcaag
      181 cacgagcttg aatgggtccg tcagaatccg aagggccgtc aggccaagtc caaggcccgt
      241 attgcgcgct tcgaggagct ctccagcgtc gagtaccagc agcgcaacga aacgaatgaa
      301 atcttcatcc ctgtcgccga gcgtctcggc aacgacgtga tcgagttcaa ccacgtttcc
      361 aagggcttcg gcgaccgcct gctcatcgac gacctgtcgt tccgcatccc cgccggcgcc
      421 atcgtcggcg tgatcggtcc gaacggcgcc ggcaagtcca cgctcttcaa gatgatcacg
      481 ggccgcgagc agcccgacag cggcgaaatc aagatcggca ccacggtcaa gcttgccgcc
      541 gtcgaccaga tgcgcgactc gctcccgaac gacaagacgg cgttcgacgc cgtggccgac
      601 ggccaggaca tcctgcaggt gggaaagttc tcgatgtcga gccgtgccta tctcggccgc
      661 ttcaacttca agggcggcga ccagcagaag ctcgtcggca gcctttccgg cggcgagcgc
      721 ggccgtctgc accttgccaa gacgcttctc gccggtggca acgtgctgct tctcgacgaa
      781 ccgtcgaacg acctcgacgt cgaaacgctg cgcgccctcg aggaggccct gctcgaattt
      841 gcgggctgcg ccctcgtgac gtctcatgac cgctggttcc tcgaccgaat cgcgacgcac
      901 atcctcgcct ttgagggcga ctcccgcgtc gtattcttcg acggcaacta caacgagtac
      961 gaagccgaca agcgccgccg tctcggcgaa gaggcctgcc agcccaagcg ccttcgcttc
     1021 aagccgctca agcactgatg aggaaatgcg cccggccgaa aggccgggcc gatgcgatga
     1081 ccgttcatct gctgacatct gcgaaaaagc ctctgctcga cttcgtgcgg cagaggcttt
     1141 tgccgttgga gccctgcgaa gtccgcaccg tgctcgttcc cgtggcgcaa gagacggcgg
     1201 aggacgtcgc gcaggcgctc ggactagttc ccggagattc gggatttgcg tcaatgccgg
     1261 gactccacgt ggtgccctgc ggcgggacga gactcgtcct cgtcagggcg gagaccgccg
     1321 ctgcggccct cagggagacg acgaccgtgc ccgacgtgct cgtttcgatg cccgaggaca
     1381 tcaacggcgg gttcagacgt ctgatggggg ctcgaacccg tctcgtcacg acggcgccgc
     1441 ttgaacgcgc gccgggtttt cttgagcctg acggagagtc gtggcgcctg aggagcgcac
     1501 ggaaggctcc tgaagagcag caggaacaac aggggggcgg aagcagcgct tcggagcgcg
     1561 tgctgatcgt gggtgcgggg cttgccggcg ccatgacggc atgggagctc gctcagcgcg
     1621 gcagtcgtgc cgtggtcgtt gatgcggggc ctgtgcccgg cagcggcgcc tcggcccttc
     1681 atgcgggact cattcatccg cactggcagg cgtcggattc gccgctcttt cagctcacgc
     1741 gagcgggctt tgaggccatg acggaggtgc ttcgagactt ccccgacgcc ttcattcccg
     1801 agggcgtcgt tgacgcggcc tcgtccgagg aggagtacga aaagtggcgg gaggccgcag
     1861 cctatggccg tcccatgcat cttccgggag acttcgcgtc gctcctcacg cgggaggagg
     1921 cgtccgagcg ggcgggactt gcactctcgc gcggcggctg gctctacccg aaggccggtt
     1981 tcgtgcatgc gggacgtctt gccagaagga tgcttgaggc ggcgcaggct caggtgctca
     2041 cgaacatgcc cgtgagcctt cgcaggcgag aggggctgtg ggaggccgtg tcggctcagg
     2101 gcgtcgtcgt tgcccgcgct ccgaaggcgg ttgtctgcgc cgcgcttgcc acaccctgcg
     2161 tgctcggcct cgcacgcggc acgatgggac tcagtccgct ttacggacgc atatcgctcc
     2221 tcagggagac ggatcttcct gaactcaggt gtgcgctcac gggtgacgga tacgtagccc
     2281 gaacggaagg cttctgcgcg gtgggcgcca cgtacgagcc gggcgaggcg cccgacgttg
     2341 cggtgcagga ggcgcatgag cacaacctgt ccaccttcga caagctcacg gggcgtcgcc
     2401 ccgacgtgct ggccgcgggc ttttacgaag gcgtccgagc cgtacccgcc gaccgcatgc
     2461 cgcttgcggg ccgcggctgg acggtcgccg agctcgaggg gcttgctttc aggggcgtgc
     2521 ccgaagcgag atcgattccc agggcgcctg gtctctggat ctgcgcgggc ttcggctccc
     2581 gcgggctcac ctgggggctt gcgtgcgcaa ggcacgtggc ggcggacgtg acgggcgacg
     2641 tgcaggcgct gcccggatcg ctcgccgcga agctcgatcc cgcgcgcttc ctcccgaagc
     2701 tgctcgcggg ctgagagagg aaggttgaaa aagatctggc cgaaaaaagc aaagggacgg
     2761 tccgtgcagg atcgtccctt tcgtcagtca ctggacgtaa ggcttacttt tggttcatct
     2821 cggaagtccg tggaacgcgg ccttgacttt taagaaaaag tacagatcgt ctcggaagcc
     2881 ataagctcgt cgcttaattt gcttgat
//
LOCUS       DBMDTI010000260         2884 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7726_length_2884_cov_2.821416, whole genome shotgun sequence.
ACCESSION   DBMDTI010000260 DBMDTI010000000
VERSION     DBMDTI010000260.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7726_length_2884_cov_2.821416"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            112..>2884
                     /gene="dnaE"
                     /locus_tag="ACZPB6_09350"
     CDS             112..>2884
                     /gene="dnaE"
                     /locus_tag="ACZPB6_09350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR1184705.1"
                     /translation="MTEPRFVHLRLHSEYSVTDGLVRIDPAIHKTLEMGGVALGITDL
                     MNVFGGLRFYTHALSAGLKPILGCDVKIKNNKFPDNPYRMTFYCMNHTGYHSLCVLLT
                     KAFLEGQDPYRGQICDEWLDVPGATDGLIALTGGPKGRLSQLIDTGSERQAGGIVGRW
                     KKLFPNRLYIELQRANRPKDENQNAFLVTVASRFDVPVVATHPVQFLEPQDFEAHEVR
                     CCIAGGFTITDPRRDKSYTREQYLKSEEQMCELFKDVPSALANSVIIAKRCNLDGVLS
                     KPQLPLFPTPDGMSLDDYMDHLSREGLEKRLAFLYDDEKVRDAKRPEYVERLDYELGI
                     IKKMQFPGYFLIVQDFINWSKTHGVPVGPGRGSGAGSLVAYSLGITDLDPLKFDLLFE
                     RFLNPERVSMPDFDVDFCQYNRDRTIEYVKETYGADAVSQIATFGTLGAKAVVRDVAR
                     VLNVPFSKADRLAKLIPMQPGKNVTLDDAIKEVPEFREAIESDDEYKELMRVAKGLEG
                     ITRNLGMHAGGVLIAPGALTDFCPLYNSDGAPENTISQFDKKDVENVGLVKFDFLGLT
                     TLSILAKCKEYIDKLYPEREFELERIPVTDEETYRCFQNANTAAVFQFESEGMRQLLR
                     QARPDRLEDLVALNALYRPGPMDLIPSFIARKFGREKVEYLDERMAPVLSETYGIMVY
                     QEQVMRVAQVVGGYSLGGADLLRRAMGKKNVEEMKRQRAVFVKGAGERGVKASVATEI
                     FNLMEKFAGYGFNKSHAAAYSYVAYQTAYLKTHFTSCFMAANLCMVMDQGDKMKALLD
                     DARANGIEILPPDINLSDWFFTAPDEKHIRFGLGGVKGLGRQQVEDMMATRIKDGPFT
                     DLFDVTARVSGLNSRNLEGLIRVGAFDSLNPDRGFLFGNVSQAMTAGQAVAASEGQDS
                     LFADDVQVKR"
BASE COUNT          570 a          854 c          867 g          593 t
ORIGIN      
        1 gacatgaagc aggcggtgat gacgcctgcg gcggcttcgt ccgtgacgtc cgccgatccc
       61 tcggttaaac tcaccgtttt gaagtaaccc tgcagagcat tgttttaggt catgactgag
      121 ccccgtttcg ttcatcttcg tcttcactcc gagtattccg tcacggacgg actcgtgcgc
      181 atcgaccccg ccattcacaa gactcttgaa atgggcgggg tggcgctcgg tattacggac
      241 ctgatgaacg tcttcggcgg cctgcgcttt tacacgcatg cgctctccgc cggtctcaag
      301 cccatcctcg gctgcgacgt caagatcaag aacaacaagt tccccgacaa tccctacagg
      361 atgacgttct actgcatgaa ccacacgggg taccactccc tgtgcgttct gctcaccaag
      421 gcgtttctgg agggacagga tccgtatcgc ggccagatct gtgacgaatg gcttgatgtg
      481 cccggtgcca cggacggcct catcgcactg acgggcggcc ccaagggacg cctgtcgcag
      541 ctcattgaca cgggcagtga gcgtcaggcg ggcggcattg tcggacgctg gaagaagctc
      601 tttccgaacc gtctctacat cgaactccag cgtgccaaca gaccgaagga cgaaaatcag
      661 aatgccttcc ttgtgacggt ggcctctaga tttgacgttc cggttgtggc gactcatccc
      721 gttcagttcc tcgagccgca ggacttcgag gcgcacgaag tgcgctgctg cattgcgggc
      781 ggcttcacga tcaccgaccc tcgtcgcgac aagagctata cgcgcgagca gtacctgaag
      841 agcgaggagc agatgtgcga gctcttcaag gacgttccga gcgcgctggc caattccgtc
      901 atcatcgcga agcgctgcaa tcttgacggg gtgctttcga agccgcagct gccgctcttc
      961 ccgacgccgg acggcatgag tctggacgac tacatggacc atctctcccg tgagggtctg
     1021 gagaagcgtc tggccttcct ttatgacgac gagaaggtga gggacgccaa gcgcccggaa
     1081 tacgtggagc gtcttgacta tgagctcggc atcatcaaga agatgcagtt cccgggctac
     1141 ttcctcatcg ttcaggactt catcaactgg tcgaagaccc acggcgtgcc cgtgggcccg
     1201 ggacgaggtt cgggcgcagg ttcgcttgtc gcctattcgc tcggcattac ggaccttgat
     1261 ccgctcaagt tcgaccttct gttcgaacgc ttcctgaatc ctgagcgcgt gtccatgcct
     1321 gactttgacg tggacttctg ccagtacaac cgcgaccgca cgatcgaata cgtgaaggag
     1381 acgtacggtg cggacgccgt gagtcagatc gcgaccttcg gcacgctggg tgccaaggcc
     1441 gtcgtgcgtg acgttgcacg cgtgctcaac gtccccttct cgaaggcgga ccgtctggcc
     1501 aagctcattc cgatgcagcc cggcaaaaac gtcacgcttg atgatgccat caaggaggtg
     1561 cccgagttcc gcgaggcgat tgagagcgac gatgagtata aggaactcat gcgtgtggcc
     1621 aaggggcttg agggcatcac ccgcaacctc ggcatgcacg cgggcggcgt gctgatcgct
     1681 ccgggggcgc ttacggattt ctgtccgctc tacaactccg atggcgctcc cgaaaatacg
     1741 atcagccagt tcgacaagaa ggacgtggag aatgtcggtc tcgtgaagtt cgacttcctg
     1801 gggcttacga cgctctccat ccttgcgaag tgcaaggagt acatcgacaa gctctatccc
     1861 gaaagggaat tcgagttgga gcgcattccc gtcacagacg aggagaccta ccgctgcttc
     1921 cagaacgcca atacggccgc cgtcttccag tttgaatcgg aaggcatgcg tcagctgctg
     1981 cgtcaggcgc gtcccgaccg tctcgaggat ctggtggcct tgaacgcact ttatcgccct
     2041 ggtccgatgg acctgattcc gtccttcatc gcccgaaagt tcgggcggga gaaggtggag
     2101 tacttggacg agcgcatggc gcccgtgctt tccgaaacct acggcatcat ggtgtatcag
     2161 gaacaggtga tgcgtgtggc ccaggtggtg ggcggttatt cgctgggcgg cgccgacctt
     2221 ctgcgccgcg ccatgggcaa gaagaacgtc gaggaaatga agcgccagcg tgcggtcttc
     2281 gtgaagggcg caggcgagcg cggcgtgaag gcgtcggttg caaccgaaat cttcaaccta
     2341 atggaaaagt tcgcaggtta cggcttcaac aagtcgcatg ctgcggccta ttcctatgtc
     2401 gcctatcaga cggcctacct caagacgcac ttcacgtcct gcttcatggc ggccaacctc
     2461 tgcatggtga tggaccaggg cgacaagatg aaggcgcttc tggatgatgc ccgtgccaac
     2521 ggcatcgaaa ttctgccgcc cgacatcaac ctctccgact ggttcttcac ggcgcccgac
     2581 gaaaagcaca ttcgcttcgg tcttggcggc gtcaagggac tgggcaggca gcaggttgag
     2641 gacatgatgg cgacccgcat caaggacggc ccctttaccg acctgttcga cgtgacggcg
     2701 cgtgtttcgg gcttgaattc ccgcaacctc gagggactca ttcgtgtggg cgcctttgac
     2761 tcgctcaatc ctgaccgagg cttcctgttc ggaaacgttt cgcaggccat gacggctggt
     2821 caggctgtgg cggccagcga ggggcaggac agcctctttg cagacgatgt gcaggtcaag
     2881 cgca
//
LOCUS       DBMDTI010000261         2874 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7762_length_2874_cov_1.560765, whole genome shotgun sequence.
ACCESSION   DBMDTI010000261 DBMDTI010000000
VERSION     DBMDTI010000261.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7762_length_2874_cov_1.560765"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(245..1030)
                     /locus_tag="ACZPB6_09355"
     CDS             complement(245..1030)
                     /locus_tag="ACZPB6_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184706.1"
                     /translation="MIVTRSDIESFKRFVPKLALAGAAVLILSAFAPSSDAAVFTARE
                     GDAPLEFTLPAHETTMVAIPADPIASAVYDRHLIDVQTDAASGMLYVLPKMEGSAMVY
                     VTCRSGETAPLHLTFTADAEPRTIILEKSVRRAEQANAPVRMPAAPLRAEGFPAEMKR
                     LVTLVLRGEETDEVSRTAMTEPAQGAAAAMKRLAPLRVRIEGIWRSRHAEAVELTVKN
                     GTIFPVEINPELLSGSVWAAAATETSLEPGDSCRLVLVENARE"
     gene            complement(1056..1622)
                     /gene="traE"
                     /locus_tag="ACZPB6_09360"
     CDS             complement(1056..1622)
                     /gene="traE"
                     /locus_tag="ACZPB6_09360"
                     /inference="COORDINATES: protein motif:HMM:NF017152.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HIR1184707.1"
                     /translation="MLYTSIVAERIGLRRSFAVIMAMSFALNFILALALLLQQPETRT
                     IVVPPTLAAEREVWTFDEAGPGAPYLERWALSVLSHAASVTPETVDSSRRVVLQHVDP
                     GFYGKLEETLIVEADRIKKDHSSTIFYPDRARVNTSDLSVEVEGVQKTLVGSTVTSSR
                     KKTWRLTFRYDAGRLFLTSLTDQPSSGS"
     gene            complement(1728..2015)
                     /gene="traL"
                     /locus_tag="ACZPB6_09365"
     CDS             complement(1728..2015)
                     /gene="traL"
                     /locus_tag="ACZPB6_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HIR1184708.1"
                     /translation="MAAPEGVRVPTGLDDPPRFLFFDADLVAVVLTVTYLGAVLDHIG
                     AGVFLGALSAYGWHKVSGLHGRGYGAALAYWHLGASSFGRTPPSSARHFVR"
     gene            complement(2015..2428)
                     /gene="traA"
                     /locus_tag="ACZPB6_09370"
     CDS             complement(2015..2428)
                     /gene="traA"
                     /locus_tag="ACZPB6_09370"
                     /inference="COORDINATES: protein motif:HMM:NF041281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraA family conjugative transfer protein"
                     /protein_id="HIR1184709.1"
                     /translation="MPKRTFALAGKRKTPPTVSNQPNVGGFLKKSDENHMKINFHQLV
                     SAVGSKKDKIAAGLLVLYAQAASAGDVQAFDKLYTQLTGWAQGSLGKSIAIIFLLVGL
                     AVGLAKGSLYAAVVSIACGLTLMIAPSVIDSIFTV"
BASE COUNT          566 a          763 c         1026 g          519 t
ORIGIN      
        1 cgggcggagc cttgcgggcg ggcttggtgt cggatgcctc gacagcaatg aaggcgatga
       61 tcgccagggc ggcgaggatc gaggcggcga gcgccttctg cttgaacctg agagtgctgt
      121 tcgagggctg ggagacgccg tcagaagacg cggagaaaat gtccttcagc tgcatgacga
      181 cactccggga agatggccgg cgtcagcgtt gcgcacggtg catgcgcaat cgttcgttct
      241 gatctcattc acgggcgttc tccacgagga cgagcctgca gctgtcgccc ggctcaagag
      301 aggtttcggt tgcggcagca gcccagaccg agcccgagag aagctcggga ttgatctcaa
      361 cgggaaagat cgttccgttt ttgacggtga gttcaaccgc ctcggcatgg cgggagcgcc
      421 agatgccttc gatgcgcaca cgaagcggtg caagcctctt catggcggcg gccgcaccct
      481 gagcgggctc ggtcatggcg gtgcgggaga cttcatcggt ctcttcgccc ctgaggacga
      541 gcgtcacaag gcgcttcatc tcggcgggga agccctcggc gcgaaggggc gcagcgggca
      601 tgcgaacggg agcattcgcc tgttcggccc tgcgcacgct cttttcgagg atgatggtgc
      661 gtggctccgc atccgcggtg aaggtgagat gcaggggcgc agtctcgccc gatctgcagg
      721 tgacgtagac catggccgag ccctccatct tcgggaggac gtagagcatg ccgctcgcgg
      781 catccgtctg aacgtcgatc aggtgacggt cgtagacggc cgaggcaatg gggtcggccg
      841 gaatggcgac catggtggtc tcgtgcgcgg gcagtgtgaa ttcaaggggt gcgtcgcctt
      901 cacgggccgt gaagacggcg gcgtcggacg acggggcgaa ggccgagagg atcaggacgg
      961 ctgcgcctgc gagcgcgagc ttcgggacga atcgcttgaa ggattcaatg tcagagcgag
     1021 tgacgatcat gagggctccg tgagggaagg ttttgtcagg acccagacga cggctggtcg
     1081 gtgagggacg tgaggaagag gcgtcctgcg tcgtagcgga acgtcagtcg ccaggtcttc
     1141 tttctcgagc tcgtgacggt gctgccgacg agcgtcttct ggacgccttc gacctcgacg
     1201 gagaggtcgg acgtgttcac gcgggcgcgg tcgggataga agatcgtgga ggagtggtcc
     1261 ttcttgatgc ggtcggcctc aacgatgagg gtctcctcga gctttccgta gaagcccggg
     1321 tcaacgtgct gaaggacgac gcggcgggag gagtcgacgg tttcgggcgt gacgcttgcc
     1381 gcatgggaga ggacggagag ggcccaacgc tcgaggtacg gcgctccggg ccccgcctcg
     1441 tcgaaggtcc agacctcgcg ctcggcggcg agcgtgggcg gaacgacgat ggtgcgcgtc
     1501 tcgggctgct gcaggagaag cgcgagggcg aggatgaagt tgagcgcaaa gctcatcgcc
     1561 atgatgacgg caaagcttcg tctcaagccg atgcgttcgg cgacgatgga tgtgtagagc
     1621 atgagaagga tccccgaggc ggctgcgcat gcgcggacgt acggctgcgg ccctggggaa
     1681 tgggggaaag tgattgacgt tcggacctga ccgtcggcgc acgggcttca gcgcacgaag
     1741 tgtctggccg agctcggggg cgtccgcccg aaggaagacg cgccgaggtg ccagtaggcg
     1801 agcgctgcgc cgtagccgcg gccgtgaagg cccgagacct tgtgccaacc gtaggccgac
     1861 agcgcgccca gaaagacgcc ggccccaatg tggtccagca ccgcaccgag ataggtcacg
     1921 gtgaggacca cggcgacgag gtccgcatcg aaaaagagaa atcgaggcgg atcgtcaagg
     1981 ccggtcggga cgcgaacgcc ctcgggtgcc gccatcagac agtaaagatg ctgtcaatga
     2041 cggagggtgc gatcatcaag gtcaagccgc aggcgatgga tacaactgca gcatagagac
     2101 tccccttggc gagcccgaca gcaaggccga caagcaggaa gatgatggcg atggacttcc
     2161 ccagggagcc ctgggcccag cccgtgagct gggtgtacag tttgtcgaag gcctgcacgt
     2221 cccccgcgga agctgcttgg gcgtacagaa caagaaggcc tgcggcgatc ttgtctttct
     2281 ttgatcccac ggcggatact agttgatgga aattgatttt catatgattt tcatctgatt
     2341 ttttgagaaa gccgccgacg ttcggttggt tcgagacggt tggcggggtt tttcgtttgc
     2401 cggcgagagc aaatgtacgc ttcggcaaaa acctttcgcc atgcgggaca ttcattaagc
     2461 ttcagccccg tttgggaaaa ccctcgaatt tgggttttca gaggaagcgg tttttactcc
     2521 tgcagtcgtt tcgagccgat cgactgccgt gcaaaggtgt caaaacagag cgggtgagcc
     2581 gctcggcccc ttcggtgagg gggagcggag cggctgagcc gtaacccatt catgggatgg
     2641 cgatcggtag aaaaacgtaa aatttcggaa gagggcgagc cagggcagcc gctttgcagg
     2701 gaaaaggtca ggggaattga gaaccggtcc ccttcagggt taacgcgaat gggctacaat
     2761 cgtgaagcaa ataaaaaccc taggacgatg gtcgttccgg gattggagga aattctcgat
     2821 gtcgcatctt ttgcaggccg ttgtcggcgc agcagtcggc gacgctcttg gcgt
//
LOCUS       DBMDTI010000262         2867 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7785_length_2867_cov_2.807664, whole genome shotgun sequence.
ACCESSION   DBMDTI010000262 DBMDTI010000000
VERSION     DBMDTI010000262.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7785_length_2867_cov_2.807664"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            132..977
                     /locus_tag="ACZPB6_09375"
     CDS             132..977
                     /locus_tag="ACZPB6_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1184710.1"
                     /translation="MERIEPSNSVKMPAMNFGVYWITDPTECEAAVLSAWRGIDTAAS
                     YLNERAVGRAVARSGIPCSELFISTKLWVQDTSEAGAERAIRRSLELLRTDTIDLYLI
                     HQPYGDIYGAWRTMERFYEAGALRAIGVSNFQPDRITDFALHQRIRPTVNQIEVNPFC
                     QQREADAVNRVLGVMPSAWAPFAEGRDGLLENPVLREIATDRGATVAQVALAWLAERG
                     IISISKTVSPARMAENLAASNVKLTPEDMAKIATLDTGTSRFFSHRDPKIVEWLCTRR
                     LPSEP"
     gene            complement(1039..2295)
                     /locus_tag="ACZPB6_09380"
     CDS             complement(1039..2295)
                     /locus_tag="ACZPB6_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR1184711.1"
                     /translation="MQRSDFEIMAPAGSRETLAAALQAGADSVYFGIGALNMRAHSAG
                     GFTIDDLNEIAALCREHGAKSYLTVNTVIYDGDLAAMREIVSAAKAAGVSAVIASDVA
                     VMQCCRELGVEVHLSTQLNIANVEALRFYAQFADVVVLARELTLRQVADIRAAIDREG
                     IKGPSGRPVRIEMFCHGALCMAVSGKCWLSLHTRGKSANRGECLQSCRRDYIAVDRER
                     GTEIALDNGYFMSPKDLKTIAFLDLMVKAGVTVFKIEGRARGPEYVRTAVECYGEALD
                     AILEGRWSEEVSKSWDERLSTVFNRGFWDGWYQGATMLERTDRYGSRATKKKLYCGKC
                     TGYYAKAGVGRFTVHGETIRPGDELLVCGPTTGAVFVRPEGLRVDDAEAAEAVKGDDV
                     TFRVPEKIRENDRLYVMRETVEDELA"
     gene            2552..>2867
                     /locus_tag="ACZPB6_09385"
     CDS             2552..>2867
                     /locus_tag="ACZPB6_09385"
                     /inference="COORDINATES: protein motif:HMM:NF015378.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR1184712.1"
                     /translation="MKKTTLAALLSAAAITVAGAAWAVEAQPAQPAPAPAPAAEAAAE
                     QPAPMVGMSDAVAVAEKEYKAKTYRANLRNSGQYGLVWDVHMVKEDETHVRAYVDAKT
                     GKV"
BASE COUNT          522 a          979 c          882 g          484 t
ORIGIN      
        1 caggccgcag gctcggcacg cacctcacgg tctacggggc cgtgcgcccg caggacgcaa
       61 aggtccgcag ctggctcgag gaaaacggct gtctctgacg cccgaaacac aatcacggac
      121 aggaatccga catggagagg attgaacctt ccaacagtgt gaagatgcct gccatgaact
      181 tcggcgtcta ctggatcacc gatcccacgg aatgcgaagc cgcagtcctc agtgcctggc
      241 gcggcatcga caccgccgcc tcctacctca acgagagggc cgtcggccgc gccgtcgctc
      301 gctcgggcat cccgtgctcc gaactcttca tctccacgaa gctctgggtg caggacactt
      361 cagaagcggg cgccgagcgc gccatcaggc gctcgctcga gctgctcaga acggacacga
      421 tcgacttgta cctgatccat cagccttatg gcgacattta tggcgcatgg cgcacgatgg
      481 agcggtttta tgaagcgggc gccctgaggg cgatcggcgt gagcaacttc cagcccgacc
      541 gcatcacgga ctttgcgctc caccagcgca ttcgcccgac ggtcaatcag attgaagtga
      601 atccgttctg ccagcagcgg gaggccgacg ccgtcaaccg cgtgctcggc gtcatgccct
      661 cggcatgggc gccctttgcc gaaggcaggg acggcctctt agagaatccc gtgctcaggg
      721 agatcgccac agaccgcggc gccacggtcg cgcaggtggc gcttgcgtgg cttgccgaac
      781 gcggcatcat ctcgatctcg aagaccgtct cgcccgcacg catggcggag aacctcgccg
      841 cctcaaacgt gaagctcacg cccgaggaca tggcgaaaat cgcgacgctc gacacgggca
      901 cgagccgctt tttctctcat cgagacccga agatcgtcga gtggctctgc actcgaaggc
      961 tgccttctga gccatgaggc ggggattctc acggcaggat ctcaactgaa cgaaacttca
     1021 gtttcgttcg ctcaatcctc aggcgagctc gtcctcgacc gtctcgcgca tgacgtagag
     1081 acggtcgttc tccctgatct tttcaggcac cctgaaggtg acgtcgtcgc ccttgacggc
     1141 ctccgcagcc tcggcgtcgt cgacgcgcag cccctcgggc ctcacgaaga cggcgcccgt
     1201 cgtcggcccg cagacgagca gttcgtcgcc cgggcggatc gtctcaccgt ggaccgtgaa
     1261 gcggccgacg cccgcctttg cgtagtagcc cgtgcacttg ccgcagtaga gcttcttctt
     1321 cgtcgcgcgc gagccgtagc ggtcggtgcg ctcgagcatc gtcgcgccct ggtaccagcc
     1381 gtcccagaac ccgcggttga agaccgtcga gagacgttcg tcccaggact tcgagacctc
     1441 ctcgctccag cgtccctcaa ggattgcatc gagcgcctcg ccgtagcact cgaccgcagt
     1501 gcgcacgtat tcgggaccgc gggcgcggcc ctcgatcttg aagacggtga cgcccgcctt
     1561 caccatcagg tccagaaagg cgatggtctt cagatccttc ggggacatga agtagccgtt
     1621 gtcgagcgca atctccgtgc cgcgctcgcg gtcgacggcg atgtagtcgc gcctgcagct
     1681 ctgcaggcac tcgcccctgt tggcgctctt gcctctcgtg tgcaggctca gccagcactt
     1741 tccgctcacg gccatgcaga gcgcgccgtg gcagaacatc tcgatgcgca cgggcctgcc
     1801 cgaggggccc ttgatgccct cgcgatcgat cgcggcgcgg atgtccgcca cctgacgcag
     1861 cgtcagctcg cgcgcgagca cgacgacgtc ggcgaactgc gcgtaaaagc gcagggcctc
     1921 gacgttcgcg atgttgagct gcgtggagag gtgcacctcg acgccgagct ccctgcagca
     1981 ctgcatgacc gcaacgtcgg aggcgatgac ggccgagacg cctgcagcct tcgccgccga
     2041 gacgatctcg cgcatggcgg cgaggtcgcc gtcgtagatg acggtgttga cggtgaggta
     2101 gcttttggcg ccgtgctcgc ggcagagggc cgcaatctca ttcaggtcgt cgatcgtgaa
     2161 gccgcccgcc gagtgcgcgc gcatgttgag cgcaccgatc ccgaagtaga cgctgtcggc
     2221 gcccgcctgc agggcggccg ccagggtttc gcgcgagccc gcaggggcca tgatttcgaa
     2281 gtcggatctc tgcatgttgt ctgcctcgtc ggatgggagc tgaaaaacgg tgaagcgtcg
     2341 attctagcgg acgcggcgac cgcactcgtg ccgcagagcc cgccgttcct gcaaaaactg
     2401 ttacctcgac tgaggaaatc ctaagtttca cccatgtgaa tcctaatttt catgggatac
     2461 actgacaata cacagaaata ggacgccgcg ccgcgcagca gagcgccgcc ctgcgtccgc
     2521 ccccgacaac gcatgccatg aggaacaagt catgaagaag accacgcttg ccgctctcct
     2581 ttccgctgcc gcgattaccg tcgccggcgc cgcctgggcc gttgaagccc agcccgccca
     2641 gcctgctccc gctcccgccc ccgccgccga agcggccgcc gagcagcccg ccccgatggt
     2701 aggcatgtcc gacgccgtcg ccgtcgccga aaaggaatac aaggccaaga cctaccgcgc
     2761 caacctgcgc aacagcggcc agtacggcct cgtctgggac gtgcacatgg tgaaggaaga
     2821 cgaaacgcac gtgcgcgcct acgtcgatgc gaagaccggc aaggtcg
//
LOCUS       DBMDTI010000263         2825 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7930_length_2825_cov_3.590308, whole genome shotgun sequence.
ACCESSION   DBMDTI010000263 DBMDTI010000000
VERSION     DBMDTI010000263.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7930_length_2825_cov_3.590308"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(72..554)
                     /gene="moaC"
                     /locus_tag="ACZPB6_09390"
     CDS             complement(72..554)
                     /gene="moaC"
                     /locus_tag="ACZPB6_09390"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR1184713.1"
                     /translation="MSELTHFDEAGQAHMVNVGEKSETHRIAVACGRIHMQPATFELV
                     AGGTAKKGDVIGIARVAAIMASKRTSDLVPLCHPIGLTRVAVDFELDEKTHAVTCTAR
                     CECYGKTGVEMEALTAVQVGLLTIYDMCKAVDRGMVMTDIRLLEKDGGKSGRWTAERA
                     "
     gene            702..2201
                     /locus_tag="ACZPB6_09395"
     CDS             702..2201
                     /locus_tag="ACZPB6_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR1184714.1"
                     /translation="MNFKAWVLLVSAMTTACLPGTALSAGQTPLDLNLPSLGTVAGAE
                     LSPADEYALGAQIMRQVRADPTYMSDPETSEYLNRLGYQLVSNANTYTYQFFFFPIRD
                     ATLNAFALPGGYIAVHTGTIINAQNESELAGVMGHEIGHVSQRHIARMIEAQKGNMAL
                     TIGSMLLAILAARAGGNSGGHAAAAVAMGSQAAMIQSQLNYSRDAEREADRVGLQTLY
                     NAGFDPKGMESFFERLHSSNRFYESAAPAYLSTHPLTVERMADMENRTRSIPPRLHRD
                     SLDFKLIQARLEVLQETRHDGWYKVRKEFQRRLKTSSGINEAVLHYGLSVAAQKLHEP
                     DEALVEARLAMKAGKSAILVRNLTRTEYDAARTAADKRKAVEDARSAVDRFPLSTMIA
                     ENYVDLLYSQNEHQKLSHFLRSNTAISQESSNYHALLARSYEKLGKKSLQYLHTGEMY
                     ALYGSTEAAVYQMTLGQKAADGDFYTMSQIDARLRELREQLLIEKERAK"
     gene            2308..>2825
                     /locus_tag="ACZPB6_09400"
     CDS             2308..>2825
                     /locus_tag="ACZPB6_09400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1184715.1"
                     /translation="MAIEDYMTVAEPTDELIETDAVVVGAGPVGLFQVFELGLLEIKC
                     HVIDSLRNVGGQCMELYPTKPIYDIPAVPVCSSYELTENLLKQNHHFGPVFHLGQEVT
                     RVEKQEDGRFYVETSTGTRFMTKVVIIAAGVGSFQPRPLRVKGIEKFEGTQLHYSCKD
                     PSVFEGKNVVICG"
BASE COUNT          562 a         1006 c          783 g          474 t
ORIGIN      
        1 atctacgaaa ggaggccggc gtgccaaatg cacgccggcc tcctttcgtt tcagtcaggg
       61 aaaaagccgc ctcaggccct ttcagccgtc cagcggcccg acttaccgcc gtccttctcg
      121 agaaggcgta tgtcggtcat caccatgcct cggtcgacgg ccttgcacat gtcgtagatc
      181 gtgagcaggc cgacctgcac ggccgtgagc gcctccatct cgacgcccgt cttgccgtag
      241 cattcgcagc gggccgtgca cgtcacggcg tgcgtcttct cgtcaagctc gaagtcgacc
      301 gccacgcggg tgagtccgat cggatggcag agcggcacga gatccgacgt gcgcttgctg
      361 gccatgatgg ccgccacgcg cgcaatgccg atcacgtcgc ccttcttggc cgtaccaccc
      421 gcgacgagct cgaacgtggc gggctgcatg tggatgcggc cgcaggccac tgcgattcgg
      481 tgcgtctcgg acttctcgcc gacgttgacc atatgggcct gaccggcctc gtcaaagtgt
      541 gtcagttcgc tcattttcgt tacctttgat cctgtaaggc gcccgaaagc ggggctccgg
      601 acgcgtccgc ccattatgat agcggtcggg acctccattt tgtctgcacg atgcgcaggc
      661 gcggcgcaca ctgtccgctc ctccgcccca aaggaatttc gatgaacttc aaagcgtggg
      721 tacttctcgt ctcggccatg acgaccgcat gcctgcccgg cacggcactg tcggcaggcc
      781 agacgccgct cgacctcaac ctgccgagtc tgggcaccgt cgcaggcgcc gagctctcac
      841 ccgccgacga atacgctctc ggcgcgcaga tcatgcggca ggttcgagcc gaccccacct
      901 acatgtcgga ccccgaaacg agcgaatacc tcaacaggct cggctaccag ctcgtcagca
      961 acgcgaacac ctacacctac cagttcttct tctttcccat ccgggacgca acgctcaacg
     1021 ccttcgcgct tccgggcggc tacatcgccg tgcacacggg caccatcatc aacgctcaga
     1081 acgaaagcga gcttgcaggc gtcatgggcc atgaaatcgg ccacgtctcc caacgccaca
     1141 tcgcgcgcat gatcgaggcc cagaagggga acatggccct cacaatcggt tccatgcttc
     1201 tggccattct cgccgcacga gcgggcggaa attcgggcgg tcacgccgcc gcggccgtcg
     1261 ccatgggctc ccaggccgcc atgatccagt cccagctcaa ctactcgaga gacgccgaac
     1321 gagaggccga ccgcgtgggt ctgcagaccc tctacaacgc aggcttcgac cccaagggca
     1381 tggagagctt cttcgagcgc ctccattcga gcaaccgctt ctacgaatcg gccgcccccg
     1441 cctacctctc cacccatccg ctcaccgtcg agcgaatggc cgacatggag aaccgcaccc
     1501 gcagcatccc gccgcgcctg caccgcgaca gcctcgactt caagctgatc caggcgcgcc
     1561 ttgaggtgct gcaggagacg cgccacgacg gctggtacaa ggtcaggaag gaattccagc
     1621 gccgcctcaa gacctcctcg ggcatcaatg aggccgtgct tcactacggc ctctcggtgg
     1681 cggcccagaa gctgcacgaa cccgacgagg ccctcgtcga ggcgcgcctc gccatgaagg
     1741 cgggcaagag cgccattctc gtgcgcaatc tcacgcgcac cgagtacgac gccgcccgca
     1801 ccgccgccga caagagaaag gccgtcgagg acgcccgcag cgccgtcgat cgctttccgc
     1861 tctccacgat gattgcggaa aactacgtcg acctcctcta cagccagaac gagcatcaga
     1921 agctcagcca cttcctgcgc agcaacaccg cgataagcca ggagagctcc aactaccatg
     1981 cactcctcgc acgctcctac gaaaagctcg gcaagaagag cctccagtac ctgcacacgg
     2041 gcgagatgta cgcgctctat ggctccaccg aggcggccgt ctaccagatg acgctcgggc
     2101 agaaggccgc cgacggcgac ttctacacca tgagccagat cgacgcacgc ctgcgcgaac
     2161 tgcgcgagca gctcctcatc gagaaggagc gcgccaagtg ataggccccg caggcgcctg
     2221 catcaggacc cgaaagcgtg cgaaaatacc gcaaccgcct tccccgaggg gaaggctcaa
     2281 ccgtttttac ccattcgaaa cagaatcatg gctattgaag actatatgac cgttgccgag
     2341 cccaccgatg agctcattga aaccgatgcc gtcgtcgtgg gcgccggtcc cgtcggcctc
     2401 ttccaggtgt tcgagctcgg cctgctcgaa atcaagtgcc acgtcatcga ctcgcttcgc
     2461 aacgtcggcg gccagtgcat ggagctctat ccgacgaagc ccatctacga cattcccgcc
     2521 gttcccgtct gctcctccta cgagctcaca gaaaacctcc tcaagcagaa ccatcacttc
     2581 ggccccgtct tccacctcgg ccaggaagtc acccgcgttg aaaagcagga ggacggccgc
     2641 ttctacgtcg agacgagcac cggcacccgc ttcatgacca aggtcgtgat catcgccgcc
     2701 ggcgtcggct ccttccagcc gcgtccgctc cgcgtcaagg gcatcgagaa gttcgagggc
     2761 acgcagcttc actacagctg caaggatccc tccgtctttg aaggcaagaa cgtcgtcatc
     2821 tgcgg
//
LOCUS       DBMDTI010000264         2824 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7933_length_2824_cov_2.286816, whole genome shotgun sequence.
ACCESSION   DBMDTI010000264 DBMDTI010000000
VERSION     DBMDTI010000264.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7933_length_2824_cov_2.286816"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="ACZPB6_09405"
     CDS             complement(<1..906)
                     /locus_tag="ACZPB6_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1184716.1"
                     /translation="MFSRFFIERPVFSWVIAIVIMLAGALSILRLPISQFPQIAPPQV
                     SISATYPGASAETIENTVTQIIEQNLTGLDGYLYMSSTSDSTGRVSITVTFEAGTNPD
                     MAQVQVQNKIQTALTTLPQVVQQMGVTIQKTSANFLMVVGFISKDHSMNASDLGDYLV
                     AHVQEPLSRVQGVGEAEVFGSEYAMRIWLDPSKMVKYKLNPSEVKAAISAQNAQDSAG
                     GIAQQPTDGKQLFTATISSASMLQTPEEFRNILLKTTEDGAKVYLRDVAEVAVGQQTY
                     MAFATYDDYPAAGMAIKLASGANAIA"
     gene            complement(928..2121)
                     /locus_tag="ACZPB6_09410"
     CDS             complement(928..2121)
                     /locus_tag="ACZPB6_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1184717.1"
                     /translation="MLSTHKTITLALMCALASTTVLTGCKDGAQQGQAAQRQMPPTEV
                     TYVTAKTTDQTLQTTLTGRTSAYYEAEVRPQVNGVLVKKLFKDGSEVKAGQQLYQIDD
                     APYVAALKSAEASLAQANANLVKARADAKRSSELVKINAVSKQSDDAAQAALKSAVAN
                     QKAAEAAVATAKVNMQYTKVLSPISGRISRSEFTEGALMAAYQATPLTRVQQLDPIYV
                     DVTQKADDLMRIRRDIASGVLKSGDNQSARVQLVFDDGSVYPHEGELTFTDVTVNEST
                     GMVNVRAVFPNPDKVLLPGLYVRANLVEGVRPDSIVIPMQCVMRDAKGNASVWVIDAE
                     NKIATRKITASRSIGTSWLVESGLKAGDRVVFEGFQRTRAGATVSPKEADASKMLDKT
                     GKSLY"
BASE COUNT          468 a          756 c         1009 g          591 t
ORIGIN      
        1 ggcgatggcg ttggcgcccg aagcgagctt gatggccatg ccggcggcgg gataatcgtc
       61 gtaggtggcg aaggccatgt aggtctgctg gccgacggcg acttcggcga cgtcgcgcag
      121 gtagaccttg gcgccgtcct cggtcgtctt gaggaggatg ttgcggaatt cctcaggcgt
      181 ctgcagcatg cttgccgacg agatggtggc cgtgaagagc tgcttgccgt cggtcggctg
      241 ctgggcgatg ccgccggcgg agtcctgggc gttctgggcc gagatggcgg ccttgacttc
      301 gctcgggttg agcttgtact tcaccatctt gctcgggtcg agccagatgc gcatggcgta
      361 ttcggagccg aatacttcgg cttcaccgac gccctgcacg cgagagagcg gctcctgaac
      421 atgggcgacg aggtagtcgc ccaagtcgga ggcgttcatg ctgtggtcct tggagatgaa
      481 gcccacgacc atcaggaagt tggcggacgt cttctgaatg gtgacgccca tctgctgcac
      541 gacctgcgga agcgtggtca gggccgtctg gatcttgttc tggacctgaa cctgagccat
      601 gtcggggttc gtgccggcct cgaaggtcac cgtgatggac acgcggcccg tcgagtcgga
      661 cgtcgacgac atgtagaggt agccgtcaag accggtgagg ttctgctcga tgatctgcgt
      721 gacggtgttt tcgatcgtct cggcggaggc gcccggatag gtggcggaga tgcttacctg
      781 cggaggcgcg atctgcggga actggctgat cggcagcctg aggatggaga gagcgcccgc
      841 gagcatgatc acgatggcga tcacccacga gaagacgggc ctctcaatga aaaagcgtga
      901 gaacatggtt ttctgcctca gtccgtatta gtagagggac ttgcccgtct tgtcgagcat
      961 cttggaggcg tcggcctcct tgggcgaaac ggtggcgccg gcgcgggtgc gctggaaacc
     1021 ctcgaagacg acgcggtcgc cggccttgag gccggactcg acgagccagc tcgtgccgat
     1081 cgagcgggag gccgtgatct tgcgggtggc gatcttgttc tcggcgtcga tcacccagac
     1141 cgaggcgttg cccttggcgt cgcgcatcac gcactgcatc ggaatgacga tggagtcggg
     1201 acgcacgccc tcgacgaggt tggcgcggac gtagaggccg gggagaagaa ccttgtccgg
     1261 attcgggaac acggcgcgga cgttgaccat gccggtggat tcgttgacgg tgacgtcggt
     1321 gaacgtcagc tcgccctcgt gcgggtagac ggagccgtcg tcaaagacga gctgaacgcg
     1381 ggcgctctgg ttgtcgccgg acttgagcac gccggaggcg atgtcgcggc ggatgcgcat
     1441 caggtcgtcg gccttctgcg tcacgtcgac gtagatcggg tcgagctgct gcacgcgcgt
     1501 gagcggcgtg gcctggtagg cggccatcag cgcgccttcc gtgaattcgg agcgggaaat
     1561 gcggcccgag atcggggaga gtaccttcgt gtactgcatg ttgaccttcg cggtcgccac
     1621 ggcggcctcg gcggccttct ggttggcgac ggcggacttg agcgcggcct gagcggcgtc
     1681 gtcggactgc ttcgagacgg cgttgatctt cacgagctcg gaggagcgct tcgcgtcggc
     1741 gcgagccttc acgaggttgg cattggcctg ggcgagcgag gcttctgcgg acttgagcgc
     1801 ggcgacgtag ggggcgtcgt cgatctgata gagttgctgg ccggccttga cttcggagcc
     1861 gtccttgaag agcttcttga cgaggacgcc gttgacctgc gggcgaacct cggcttcata
     1921 gtaggcgctg gtgcggcccg tcagggtcgt ctgaagcgtc tggtcggtcg tcttggccgt
     1981 cacgtaggtg acttccgtcg gaggcatctg ccgctgggcg gcctgcccct gctgggcgcc
     2041 gtccttgcag ccggtgagca cggtcgtgga agccagggcg cacatcagcg ccagggtgat
     2101 ggttttgtgc gttgacaaca tgtcaatgta ccttctggtt tttgtcgaaa acggttgtat
     2161 caggtcgtcg gaaagcgccg tgttgcggga ctctttcctg aaaacctgtt cagtaatggc
     2221 tgaacggttg ttcaggattc tagcaaacct tctgaacgaa cgttcaggat tgtaccaaat
     2281 cgaaacggtg ccgaacgtaa agattcttcc atttccgagt tgtttttccg atgtttcgga
     2341 cacgtgcttc atcattggga tcgctactgt tcgaggggaa gaggaggttt tcggggcttc
     2401 cgcgctgtaa aacggcgcgc tgtctcagta gtgtcctctg caatcccaca ggtatgaacg
     2461 gatcgtcagg ttttgggatg ttcgaagaag atagccggct aatcaagtga ttttgccgaa
     2521 agttgaaggc agaagacgga agtccggatc ttttttgttg agggagaggg cttttgacgg
     2581 cgtcgcgggg accccggcgc tcgcggtttc gtctgcggca tcacgagggc gtgctgcgca
     2641 tggacgacgc gcgcagcgat gagcctgcgc ccgtgggttg acggcggggt tcgatgcaaa
     2701 tccgcccgcg ggagaggcgt tctcctgcgg gcggcgggtc acgaaaggtt gttcaccggt
     2761 ccttcatcgg agggcttcgg ccgttcgggc gatgaagacg acgaagtcct gcacgaagcg
     2821 ctgc
//
LOCUS       DBMDTI010000265         2821 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7941_length_2821_cov_3.613235, whole genome shotgun sequence.
ACCESSION   DBMDTI010000265 DBMDTI010000000
VERSION     DBMDTI010000265.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7941_length_2821_cov_3.613235"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="ACZPB6_09415"
     CDS             <1..435
                     /locus_tag="ACZPB6_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HIR1184718.1"
                     /translation="PCLIPVWPGIMSPSAVLFAGLALILRDAVQSLLGKRWTLVAIAA
                     GAALSGILSEPAIILGSTCAFLFSELADFAVYTPLRERHLTLAIVLSGLVGSIVDSVI
                     FLSLAFGSLDFIVGQVIGKFWMSLIAGAMIIMWRSTRRAQAA"
     gene            620..1618
                     /gene="hemH"
                     /locus_tag="ACZPB6_09420"
     CDS             620..1618
                     /gene="hemH"
                     /locus_tag="ACZPB6_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008541372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR1184719.1"
                     /translation="MANSNKIALLLINTGSPDAPTEEAVRRYLAEFLGDRRIVELPRW
                     KWWPILHGIILRTRPKKSAERYRGVWTDEGSPLIVHTRRTAGALEAELGIPVYWSMRY
                     GSPSTASVLDRMKADGVGRVLVMPMFAQYASQTTAACLDGVSEYQLSHVDAPAVRTIH
                     DYHDDPAYIDALAAHVRAYWDEHGSPVSTGGRLVMSFHGIPQASSNRGDVYEAQCLRT
                     ASLLAERLGLEEGAWSVCFQSRFGRDEWLRPYTIDSVRELAEAGVPRIDVVCPGFAAD
                     CLETIEEIDDELRREYLAAFRDDAGAEFHYIAALNDSPEAVKAYASIVRREAAGWI"
     gene            1755..2372
                     /gene="grpE"
                     /locus_tag="ACZPB6_09425"
     CDS             1755..2372
                     /gene="grpE"
                     /locus_tag="ACZPB6_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR1184720.1"
                     /translation="MTENTQNQEVKEEAGCCCQGKCAEEGQACGCEGKEACTGEEAKQ
                     PETPEQTIETLQTALKLSEAKVMEHYDLYVRAMAELENTRRRSSEEVQKARKFGIEKF
                     AENLLPVVDSLEKALEVTKNEEENPVREGMLATYRQFMHALDVSGMKPVDPKGEAFDP
                     HQHQAIAMVPAPEGVASGTVVEVFQRGWVIADRVLRPAMVSVAQG"
     gene            2706..>2821
                     /locus_tag="ACZPB6_09430"
     CDS             2706..>2821
                     /locus_tag="ACZPB6_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR1184721.1"
                     /translation="MAKIIGIDLGTTNSAVAVMEGDNIKVIENSEGARTTPS"
BASE COUNT          533 a          893 c          864 g          531 t
ORIGIN      
        1 ccctgcctca ttcccgtctg gcccggcatc atgtcgccgt ccgccgtgct cttcgccggt
       61 cttgcgctca ttctgcgcga cgcggtccag agcctgctcg gcaagcgctg gacgctggtc
      121 gcgattgccg caggcgccgc gctctccggc attctctccg agcccgcgat cattctcggc
      181 tcgacctgcg ccttcctctt ctccgagctg gccgactttg ccgtctacac gccgcttcgc
      241 gaacgtcatc tgacgctggc catcgtgctc tccggcctcg tgggctcgat cgtcgactcc
      301 gtgatcttcc tctcgctcgc cttcggctcc cttgacttca tcgtgggtca ggtgatcggc
      361 aagttctgga tgagccttat tgcgggcgcc atgatcatca tgtggcgcag tacccgccgc
      421 gcacaggccg cctgaagaag cacctcaacc tgattccagc agccgccagg gtacgcccta
      481 ggcggctgtt ttcgttcatg acgactgcgg acggactgcg gacgaggccc tttgcggcgc
      541 ggcgtgaaaa agcgttcctt aagaaaggtg tcccagaatg gggtacaacc acgaatccgt
      601 ttccagacct caccgttcca tggctaattc gaacaaaatc gcgcttcttt tgatcaatac
      661 ggggagcccc gacgccccga ccgaagaggc cgtccgccgc tatctggccg aattcctcgg
      721 cgacaggcgc atcgtcgagc ttccccgatg gaagtggtgg ccgattctgc acggcatcat
      781 tctgcgcacg cgtccgaaga agtccgccga acgatacaga ggcgtctgga ccgacgaagg
      841 ctcgccgctc atcgtgcaca cgaggcgcac ggccggggcg cttgaggctg agctcgggat
      901 tccggtctac tggtccatgc gctacggcag cccgtcgacg gcgtccgtcc tcgacaggat
      961 gaaggccgac ggcgtcggcc gcgtcctcgt catgcccatg ttcgcgcagt atgcctccca
     1021 gacgacggcg gcctgtctgg acggcgtgag cgaataccag ctttctcatg tcgatgcgcc
     1081 tgcggtgcgc acgattcacg actatcacga cgatcccgcc tacatcgacg cgcttgccgc
     1141 gcacgtgcgc gcctattggg acgagcacgg ctctcccgtg agcacgggcg gccgcctcgt
     1201 catgagcttc cacggcatcc cacaggcaag cagcaaccgc ggcgacgtct acgaggcgca
     1261 gtgcctgcgc actgcgtcgc ttctcgccga aaggctcggc cttgaggagg gcgcgtggag
     1321 cgtgtgcttc cagtcccgct tcggccgcga cgagtggctt cgtccgtata ccatcgacag
     1381 cgtcagggag ctggccgaag ccggcgtgcc gcgcatcgac gtcgtgtgtc cgggcttcgc
     1441 tgccgactgt ctggagacga tcgaggagat cgatgacgag cttcgccgcg agtatctggc
     1501 cgccttcagg gacgatgcag gggcggaatt ccactacatt gccgcactca acgactcgcc
     1561 cgaggcggtg aaggcctacg cctcgatcgt gaggcgcgag gctgcgggct ggatctgacg
     1621 cacggacggc gcatgagcgg acttgaaaaa cgccgcaccg tccccatata agaagcaatc
     1681 tattgggccc acggagcgga ctcccgggcc gacccagttt ccaggcgtct ggcgacgctc
     1741 atacggagtg acatatgact gaaaatactc agaaccagga agtgaaggaa gaagccggct
     1801 gctgctgcca gggcaagtgc gccgaagagg gccaggcctg cggctgcgag ggcaaggaag
     1861 cctgtacggg tgaagaggcc aagcagcccg aaacgcccga gcagacgatc gaaacgcttc
     1921 agacggctct gaaactctcc gaagccaagg tgatggagca ctacgacctc tacgtgcgcg
     1981 ccatggcgga gcttgaaaac acccgccgcc gttcctccga ggaggttcag aaggcccgca
     2041 agttcggcat cgagaagttt gccgaaaacc tgctgcccgt cgtcgacagc cttgagaagg
     2101 ccctcgaggt cacgaagaat gaggaggaga accccgtccg tgaaggcatg ctcgccacgt
     2161 accgccagtt catgcacgcc ctcgacgtct ccggcatgaa acccgttgat ccgaagggcg
     2221 aggcgttcga tccgcaccag catcaggcca tcgcgatggt gcccgctccc gaaggcgtcg
     2281 cctccggcac cgtggtcgaa gtcttccagc gcggctgggt gatcgccgac cgcgttctgc
     2341 gccccgccat ggtgagcgtc gctcagggct gagaagcccg aacacagccg ctctgatcag
     2401 aaggcgtccc gtcgggggcg ccttttttct tgatgggcct tagaatggaa aagtgatgtt
     2461 tttcccttgc ggggcaaggc cttgcggtgg tggatgtaat tatttttgac aaaccttcgg
     2521 cagaaggctt gaaaaaaaag ggggcatccc catataggga ttaaggtcgt tcgcaggctg
     2581 aggagattcc gcctgcggac acgttagttt ttgaacggtc cgaacgctcc tgcgtgtgcc
     2641 atgtgccgcg cctcaagatg acgacgtttc ggacgaaagg ttttctttta gaggattaaa
     2701 ccactatggc gaagatcatt ggtattgacc ttggcaccac caattcggcc gttgccgtga
     2761 tggaaggcga caacatcaag gttattgaaa acagcgaagg cgcccgcacg accccgtcca
     2821 t
//
LOCUS       DBMDTI010000266         2817 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_7955_length_2817_cov_3.067010, whole genome shotgun sequence.
ACCESSION   DBMDTI010000266 DBMDTI010000000
VERSION     DBMDTI010000266.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7955_length_2817_cov_3.067010"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(137..757)
                     /locus_tag="ACZPB6_09435"
     CDS             complement(137..757)
                     /locus_tag="ACZPB6_09435"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR1184722.1"
                     /translation="MFTGIIQAIGKVREPEPLGNGVRLTICAPDLGLDEVRIGDSIAV
                     NGACMTVVEKTDTEFKVDVSAESLSKTTGLDTFGEVNLEKAMRLGDRVDGHLVSGHVD
                     GVGQVESMEQVAESWRLVIRAPRVLSPYLAYKGSITVNGVSLTINKVEDTAMDTLVTI
                     NLIPHTVEVTTLKHLKAQSYVNLEIDTIARYIERMMSLRNDEDSLF"
     gene            complement(764..1882)
                     /gene="ribD"
                     /locus_tag="ACZPB6_09440"
     CDS             complement(764..1882)
                     /gene="ribD"
                     /locus_tag="ACZPB6_09440"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR1184723.1"
                     /translation="MTECAVDEGRVKDDDFWMGLALEEAAKARRLSPPNPAVGAVIVR
                     DGRVIGRGFTQQTGGPHAEVMALRDAAARGESVEGATIYVTLEPCSHWGRTPPCALAI
                     VEHRIGRVVTSVADPNPQVAGRGLKMIRDAGIEVTEGVRSKEGWLSNRGFLTRMTTGR
                     PWVRMKCAATLDGRTAFPDGRSQWITGPAAREDSQYWRAVSGAVVTGIGTVLADNPKM
                     TVRLPDQVRFPFRVVVDPRLETPVGAEILKGGGTVLVCAQAGEEAEAALVAAGAEVLR
                     LPDAAHPGRVDLGALLDELGRRQVNEVHLEAGAGLNGSFLSEGLVDEIVLYTAPCLFG
                     SGMPVASVPLPEEPGLAHRWTIEDFTQIGADLRVILRR"
     gene            complement(2032..2487)
                     /gene="nrdR"
                     /locus_tag="ACZPB6_09445"
                     /pseudo
     CDS             complement(2032..2487)
                     /gene="nrdR"
                     /locus_tag="ACZPB6_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
BASE COUNT          498 a          955 c          862 g          502 t
ORIGIN      
        1 ttattttaac gcgaaagacc tgcgtgacgc agggcgggcg ggtgcgggcg ccgttttttc
       61 ggccccgctg tcgaaaaacg gcgcccttca ccagaagaac gccgcctttt catgatgcgg
      121 aacgccttct tcctgatcag aagagggagt cctcgtcgtt acgaagcgac atcatgcgct
      181 cgatgtagcg ggcgatcgtg tcgatctcaa gattgacgta gctctgggcc ttgaggtgct
      241 tgagcgtcgt cacctcgacc gtgtgcggga tgagattgat cgtcacgagc gtgtccatcg
      301 ccgtatcctc gaccttgttg atcgtaagcg agacgccgtt gaccgtgatc gagcccttgt
      361 aggcgagata cggagagagg acgcgggggg cgcggatgac gaggcgccac gattcggcga
      421 cctgctccat gctttcaacc tgacccacgc cgtcgacgtg accgctcacg agatggccgt
      481 cgacgcggtc gccgagacgc atggccttct cgaggttgac ctcgccgaac gtgtcgaggc
      541 ccgtagtctt cgagaggctc tcggcggaga cgtcgacctt gaattccgtg tccgtcttct
      601 cgacgacggt catgcacgcg ccgttgacgg cgatcgagtc gccgatgcga acctcgtcaa
      661 gaccgagatc gggagcgcag atcgtcaggc ggacgccgtt gccgagaggt tccggttcac
      721 gaaccttgcc gatggcctga ataatgccgg taaacattta gagtcatctc ctcaaaatca
      781 cacgcagatc ggcaccgatc tgagtaaagt cttcaatcgt ccagcggtgc gccagaccgg
      841 gttcctcggg aagcggaacg cttgcaacgg gcatccccga gccgaaaagg cagggagcag
      901 tatacagcac gatctcgtcg acaagtccct cggagagaaa gctcccgttg agccccgcac
      961 ccgcctcgag atggacctcg ttgacctgcc ttcgtccaag ctcgtcgaga agcgcaccga
     1021 ggtccacgcg tcccggatgc gccgcatcgg gaagcctcag cacctcggcg cctgcggcga
     1081 cgagcgccgc ctcggcctct tcgccggcct gcgcacagac gaggaccgtg ccgccgccct
     1141 tgagaatctc cgccccgacg ggcgtctcaa gcctcggatc cacgaccacg cggaacggga
     1201 acctcacctg atcagggagc ctcaccgtca tcttcggatt gtcggcgagc accgtgccga
     1261 tgcccgtcac gaccgccccc gacaccgcac gccagtactg agagtcctcg cgcgccgcag
     1321 ggcccgtgat ccactgggat cggccgtcgg gaaaggccgt gcggccgtcg agcgtcgcgg
     1381 cgcacttcat cctcacccag ggcctgcccg tcgtcatacg ggtgagaaag cccctgttgg
     1441 agagccatcc ctctttgctt cggacgcctt cggtcacctc gatgcccgca tcgcggatca
     1501 tcttcagacc ccgccccgcc acctgggggt tcgggtcggc gaccgacgtc acgacgcggc
     1561 cgatcctgtg ctcgacgatc gcaagcgcac agggcggcgt gcgcccccag tgcgagcagg
     1621 gctcgagcgt cacgtagatc gtcgcgccct caacgctttc tccacgcgcg gcggcatccc
     1681 tcagagccat cacctcggcg tgcggaccgc ccgtctgctg cgtgaagccc cggccgatca
     1741 cccggccgtc cctcacgatg acggcgccga ccgcaggatt gggcggagaa agcctgcggg
     1801 ccttcgcggc ctcctcaagc gcaagcccca tccagaagtc gtcgtccttc acgcgacctt
     1861 catccacagc gcattccgtc atttggcctc ctccgcctcc gctgccttcc tctccgcatc
     1921 cctgcgggcg cggcgctcgg cggcggcctt tttcttcgcc tcgcgggcgg cgacctcggc
     1981 ctcgtgctcg agcctgaact tcctcagcac gtcgaacgcg ccgtcggtgc ttccgtcggg
     2041 cacggccgca ttctgaatca tcgatgcaaa ggcaagcgga tcgccgatgt tgaagtacac
     2101 cgacgcaaac ctgatgtagg cgatggtgtc gagcttctgc agggcgccga gcacgagctc
     2161 gcccacgacg ctcgactcga tctcgcggtc tccgagcatg atcatcgcct gctcgacgga
     2221 gtcgaccgcc tcctcaagct gctcgggcgt cacgggacgc ttgcgcagcg ccagcgtcat
     2281 cgaggcctga agcttcgccc tgttgtagtc ggtgcgctgg ccgttgcgct tgacgaccgc
     2341 gggaatgggc aggtgcgccc tctcgaaggt cgtgaagcgc ttgccgcagg ccggacactt
     2401 gcggcggcga cggatcgcgt acccctcctc aggggcgcgc gaatcgatca cctgggtatc
     2461 cggattctgg cagaagggac atcgcatgag gagactcctt gcgtacggtg gaagcccgtc
     2521 ccgaaccgca tcggccggca gacacttcca aatcaggaac aagtgtccga aagtttagcg
     2581 cccgcccgca ggcaaactca agtccccaaa cacaaaataa gggccaccca atcactcgaa
     2641 agggtagccc ttgtgttttc atgcgttttc ggggagaccc gaagcctccc ctgcggccgc
     2701 ctgagcgcct tagtcaccgt agaccgggaa ggcggcggtg aggttcgcca cttcggcctt
     2761 gacgcgttcg aagacggcct ggtcttcagg cgcctcgagc agatcgacga tcaggtt
//
LOCUS       DBMDTI010000267         2796 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8026_length_2796_cov_3.148423, whole genome shotgun sequence.
ACCESSION   DBMDTI010000267 DBMDTI010000000
VERSION     DBMDTI010000267.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8026_length_2796_cov_3.148423"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(111..2573)
                     /locus_tag="ACZPB6_09450"
     CDS             complement(111..2573)
                     /locus_tag="ACZPB6_09450"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HIR1184724.1"
                     /translation="MASIFKITASAAVVATALYAAAGYLAVPTLVKSTLADTLGKQLG
                     RSVTVTNVAFNPWTWEFTLEGLTVAGKNNNPPLLSLDRFYVNASAQTLVELAPVIDEV
                     TVDGLNAVVALDDEDMRRLMNGHGSAEGGSSEKASSESSSLPSFAVYNISVKKSSLQV
                     LDRARAIDQSVTDFNLELPFVSTLANSKSSLVTPKLSMKLNGTPIFATGSTQPFGTTL
                     SARLNMKISNLDLAPVFKLVPALNTPSLMLENGRLSTEVNVVFRNATSGKPGKMLVSG
                     TVNVNDLTAVQQVSNRVQPLASFKKASLQLTELDLLEHQASVGSIVIQDPKVHFVNTP
                     SGLNVSQTAQGFSSGAAADSGKDSGSNSGWHWRVDSVQIRNGNAAWNDSTVRPAAKIN
                     VTALNATVTGLTNAANAKPAKAEAGAKVFGGMLSLAADVTPAPLVVNATLKASGLQAA
                     QLASYIRNATGFDASGAVSLNVKAAVKGSAVTGSGTASLTNLQVKQGKSTLVSARTAS
                     VKLGSLDTAKQSVNVDSILLDTAVVNAVMTSAGLNLMPSKAGAAKKAAQKDADAKTTE
                     KAGKLWQWKVASADVKNSRFNFRDTSVKPNAAITVSDINASVKNLSSAEGAAATVKMS
                     AGTAGGTLAADGTVTLSPMTADVSTTLSGINMKSLSSVMTAYAGIGAQSGTLESKGRF
                     GLASEKDKQVASWAGDIRMAKFNMLNAKGRSLMTWNNAALTGLAVKTTDPVTLCVKKA
                     EIDQPGTRETKAIRELSGLASLLTGKDKYAKKTEKYLGGSIVLNGVRYENGRFSAEGI
                     DGNAVATALLTKLSGAMSTKLQ"
BASE COUNT          436 a          772 c         1009 g          579 t
ORIGIN      
        1 taggaagttc tctgaggtta ttgataaccg cccactgcgg atccgcacgg tgggtggtgt
       61 ggaaggggct gaggatcaaa agtcctcacc cctatccgat tgggcggcga tcactggagc
      121 ttcgtgctca tggcgcctga aagcttggtg aggagcgccg tggcgacggc gtttccgtca
      181 atgccttcgg cggagaacct gccgttttca tagcgcacgc cgttgaggac gatgctgccg
      241 ccgaggtact tctcggtctt cttggcgtac ttgtccttgc ccgtcaacag gctggcgagg
      301 ccagaaagct cgcgtatggc cttcgtctcc ctggtgccgg gctggtcgat ttcggccttc
      361 ttgacgcaaa gcgtgaccgg gtcggtcgtc ttgactgcaa ggcccgtgag ggcggcgttg
      421 ttccatgtca tgaggctgcg gcccttggcg ttgagcatgt tgaacttcgc catgcggatg
      481 tcgcccgccc agctcgcaac ctgcttgtcc ttttcggacg caaggccgaa cctgccctta
      541 ctctcgagcg tgccgctctg cgcgccgatg ccggcgtagg ccgtcatgac ggaggagagc
      601 gacttcatgt tgatgcccga cagcgtcgtc gagacgtcgg cggtcatggg cgagagcgtc
      661 acggtgccgt ctgccgcaag ggtgccgccc gcagtgcctg cggacatctt cacggtggcg
      721 gcagcgcctt cggccgagga gaggttcttc accgaggcgt tgatgtcgga gaccgtgatg
      781 gcggcgttgg gcttgacgct cgtgtcgcgg aagttgaatc tggagttctt cacgtcggcc
      841 gaagcgacct tccactgcca gagcttgccc gccttttcgg tggtcttcgc atcggcgtcc
      901 ttctgggccg ccttcttggc tgcgcctgcc ttggagggca tgagattgag gccggcggag
      961 gtcatgacgg cgttgacgac ggccgtgtcg agcaggatgc tgtcgacgtt gacggactgc
     1021 ttcgccgtgt caaggctgcc gagcttgacg gaggcggttc tggcggagac gagcgtcgac
     1081 ttgccctgtt tcacctgaag gttggtaagg gaggcggtgc cgctgcccgt gacggcggag
     1141 cccttgacgg ctgccttgac gttgaggcta acggcgcccg aggcatcgaa gcccgttgcg
     1201 ttcctgatgt aggaggcgag ctgtgcggcc tgcaggccgc tggccttgag ggtggcgttg
     1261 accacgagcg gtgcaggcgt gacgtccgcc gcaagcgaga gcatgccgcc gaaaaccttt
     1321 gcgcccgctt cggccttcgc gggcttggcg ttcgctgcgt tcgtcaggcc cgtgaccgtg
     1381 gcgttgaggg ccgtgacgtt gattttggcg gcgggcctga cggtcgagtc gttccaggcg
     1441 gcgttgccgt tgcggatctg cacggagtcc acgcgccagt gccagccgga gttgctgccg
     1501 gaatccttgc ccgagtccgc agcggcgccc gacgagaagc cttgggcggt ctgggagacg
     1561 ttgaggccgg agggcgtgtt gacgaagtgg accttcggat cctgaatgac gatgctgccg
     1621 acggaggcct gatgttcgag aaggtccaac tcggtgagct gcaggctcgc cttcttgaag
     1681 gaggcgagcg gctggacgcg gttgctgacc tgctggacgg cggtgaggtc gttgacgttc
     1741 acggtgcccg agacgagcat cttgccgggc ttgccgctcg tagcattgcg gaagacgacg
     1801 ttgacctcgg tcgagagacg gccgttttca agcatgaggc tcggcgtatt gagcgcgggc
     1861 acgagcttga agacgggggc gaggtccaga ttcgagatct tcatgttgag gcgcgccgag
     1921 agcgtggtgc cgaagggctg cgtggagccc gtggcgaaga tcggcgtgcc gttgagcttc
     1981 atggagagct tgggcgtgac gaggctgctc ttggagttgg cgagcgtgct cacgaacgga
     2041 agctcgaggt tgaagtcggt gaccgactgg tcgatggcgc gtgcgcggtc gaggacctga
     2101 agggaggatt ttttgacgct gatgttgtag accgcgaagg agggaaggct gctgctttcc
     2161 gaggaggcct tttccgacga tccgccttcg gccgaaccgt ggccgttcat gagcctgcgc
     2221 atgtcctcgt catcgagtgc gacgacggcg ttgagcccgt cgacggtcac ttcgtcaatg
     2281 acgggcgcaa gctcgacgag cgtctgggcc gacgcattca cgtagaagcg gtcgagcgag
     2341 agaagcggag ggttgttgtt cttgcctgca accgtgagcc cctcgagcgt gaactcccag
     2401 gtccacggat tgaaggcgac atttgtgacg gtcacgctgc gtccgagctg cttgccgagc
     2461 gtgtcggcga gcgtcgactt gacgagcgtg ggcaccgcga gatagccggc ggctgcgtaa
     2521 agcgccgtgg cgacgacggc cgcgcttgcc gtgatcttga aaatgctggc catgtcctgc
     2581 cctccctgac taggttcccc tgttctgtac attataggtg ccagttcaga ggctccgatt
     2641 ggcacgatgg gggtgaaaac accgccaacg cttctgcccg aggaagttca gcgggttttg
     2701 cgatatttcg acattttgtt tccgcggggc gccgtgcacg acgtagggaa gcgaaagggc
     2761 ggggtgtgat gcgcaggaag ggacggcgga gcgggg
//
LOCUS       DBMDTI010000268         2774 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8096_length_2774_cov_1.609053, whole genome shotgun sequence.
ACCESSION   DBMDTI010000268 DBMDTI010000000
VERSION     DBMDTI010000268.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8096_length_2774_cov_1.609053"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2114
                     /locus_tag="ACZPB6_09455"
     CDS             <1..2114
                     /locus_tag="ACZPB6_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015474 - autotransporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter family protein"
                     /protein_id="HIR1184725.1"
                     /translation="PSGEDKDTPDMKLYLKAGLKAIQDGKAAIVGGGLSIGGNYGVGH
                     ECYNDALSAVDTANVTLGAGSLKGNVYLGGRVLEDGANTKGVAKATVGAGTLTIYKDF
                     NFLKADAVIDGSGAESATLNFVNGYDFSKAEASEVALLDLPARIVIKGFDKLDSGDLV
                     TGANYDMGDKTSVEVTGNYEFGKILNATATQTMTVKEGGALAVQSAQALANNFVVEEG
                     VLALGTTAKTADAREGLGRYNEKAGLYLSGTVNLTNASINVGETKAKNGVNIGSNGTL
                     IVDASVDDGQEGNLSTDVTGKITGEKGSTIHYVHVADKGVVEVEHEGFTTVTVDNLLY
                     KVVKVENEDNQYTFKIATGSELAGTGLEHFDGAALDGMADNEVIGSLLDQANTAITSG
                     SQREARLNGALNLAAAGGVQTAGIEAATMGIDQATKRAALTNVFKDGWTGFAEVTGTS
                     LKMGGDSGALETKTELGGITVGGEYTMGDMTFGVMGNFGTGDVEGEGDNNGAKNDVDY
                     YGVQGYTAKRMGQFNLVGQMGLMTTKNDVTSADGDSADIDATIFTIGARGEMAFQISK
                     SCQAVPYVGLNYLRVATDGYNTAKGLSVDDMDQNLVSMPIGVAFSGNMDLASGWTLRP
                     TVDVAYVHTFGDTDVEATTKFGAAAINTNLDVWSENVGRVGFGLEARKDALSFGGQIG
                     GAFGDNDHREFYGQLNMKYLF"
     gene            2384..>2774
                     /locus_tag="ACZPB6_09460"
     CDS             2384..>2774
                     /locus_tag="ACZPB6_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR1184726.1"
                     /translation="MTEFAAHVEDFCTRLFEDIRKLTADTCGVTREGYTPEEDKVHER
                     LAEAAREIGLEVHADDALNLWMTLPGRNRDLPAFVSGSHTDSVPLGGNYDGLAGIVAP
                     LAVAKYLKDANRIPERDFCIVAFRMEEC"
BASE COUNT          591 a          877 c          789 g          517 t
ORIGIN      
        1 atcccagcgg tgaagacaag gatacgcctg acatgaagct gtacctcaag gccgggctca
       61 aggccattca ggacggcaag gctgccatcg tcggcggcgg tctctcgatc ggcggcaact
      121 atggcgttgg tcatgagtgc tacaacgacg ccctctctgc cgtggacacg gccaacgtca
      181 cgctcggagc aggcagcctc aagggcaacg tctaccttgg cggtcgcgtg cttgaggatg
      241 gcgccaatac caagggcgta gccaaggcaa cggttggcgc cggtacgctc acaatctaca
      301 aggacttcaa cttccttaag gctgatgccg tcatcgacgg ttccggcgct gaatcggcaa
      361 ccctcaactt cgtgaacggc tatgacttct ccaaggctga agcctctgaa gtcgccctgc
      421 tcgacctgcc tgcccgcatc gtgatcaagg gcttcgacaa gctcgattcc ggtgaccttg
      481 ttaccggtgc gaactatgac atgggtgaca agaccagcgt cgaggtcacc ggcaattacg
      541 agtttggcaa aattctcaat gctaccgcca cgcagacgat gaccgtcaag gaaggcggtg
      601 ccctcgccgt tcagtcggct caggcacttg ccaacaactt cgtggtcgag gaaggcgtgc
      661 ttgctcttgg cacgactgcc aagacggctg acgcccgcga aggtctcggc aggtacaacg
      721 aaaaggcagg cctctatctg agcggcacgg tcaatctcac caacgccagc atcaacgttg
      781 gcgagaccaa agccaagaat ggcgtgaaca ttggttccaa cggcacgctg attgttgatg
      841 cctctgttga tgacggtcag gaaggcaatc tttccaccga cgtgaccggc aagatcactg
      901 gcgaaaaggg ttccacgatt cattacgtgc acgtggccga caagggtgtc gttgaggtcg
      961 aacatgaagg ctttacgacc gtcacggtcg acaacctcct ctacaaggtt gtcaaggttg
     1021 aaaacgagga caaccagtac acgttcaaga tcgccacggg ttccgaactc gccggtacgg
     1081 gccttgaaca ctttgacggc gctgcccttg acggcatggc cgacaacgaa gtgatcggtt
     1141 ccctccttga ccaggccaac actgccatca cgtccggcag ccagcgcgaa gcccgcctca
     1201 acggcgctct caacctcgct gctgcaggcg gtgtccagac ggccggcatt gaagccgcca
     1261 cgatgggcat tgatcaggcc acgaagcgtg ctgcgctcac caacgtcttc aaggacggtt
     1321 ggacgggttt cgctgaagtg acgggcacct ccctcaagat gggcggcgac tccggcgctc
     1381 tcgaaacgaa gaccgagctc ggcggcatca cggtcggcgg tgaatacacg atgggcgaca
     1441 tgacgttcgg cgtcatgggc aacttcggaa ccggcgacgt cgaaggcgaa ggcgacaaca
     1501 acggcgccaa gaacgatgtg gactactacg gcgttcaggg ctacactgcc aagcgcatgg
     1561 gccagttcaa cctcgtcggc cagatgggcc tcatgacgac gaagaacgac gtcacgtccg
     1621 ctgatggcga ctccgccgac atcgacgcca cgatcttcac gatcggtgcc cgcggcgaaa
     1681 tggccttcca gatcagcaag tcctgccaag ctgtcccgta cgtcggcctc aactacctgc
     1741 gcgtcgccac tgacggctac aacaccgcca agggcctctc cgtggatgac atggaccaga
     1801 acctcgtgag catgccgatc ggcgtggcct tctcgggcaa catggacctc gcctccggct
     1861 ggacgctccg tccgacggtc gacgtggcct atgtccacac gttcggtgac acggacgtcg
     1921 aagccacgac gaagttcggc gctgccgcca tcaacaccaa cctcgacgtc tggagcgaaa
     1981 acgtcggccg cgtgggcttc ggtcttgaag cccgcaagga cgccctctcc ttcggcggcc
     2041 agatcggtgg tgccttcggc gacaacgacc atcgcgagtt ctatggccag ctcaacatga
     2101 agtatctctt ctgatactga tcgtctccct ctccgccgcc ttctccctct ctccatccgg
     2161 ggcggcgggg atccccgcct ccggcatcct tcggaatgcc ggaggttttt tgtaccttgc
     2221 aatccgcact tcgactcccc gcaggattcg tcattgggag ctgctccgca gaacccttca
     2281 tcagctcagg tccttcgagg ggtacacttg aacaatcaat ccaatccaac cgatccctac
     2341 tccactcact ccagccgaaa ccaacaggaa gaggacccgc ctcatgactg aattcgccgc
     2401 ccacgttgaa gacttctgca ctcgtctctt tgaagacatc cgcaagctca ccgccgacac
     2461 ctgcggcgtc acgcgcgagg ggtatacgcc cgaagaagac aaggtgcacg agagactcgc
     2521 cgaggccgcg cgcgagatcg gtcttgaagt ccatgcggac gatgcgctca acctctggat
     2581 gactttgcca ggtcgcaaca gggatttgcc agccttcgtc tcgggatcgc acaccgactc
     2641 tgttccgctg ggcggcaatt acgacgggct cgctggcatc gtcgcgccgc tcgccgtggc
     2701 caaatatctg aaggatgcaa atagaattcc tgagcgggac ttctgtattg ttgcattccg
     2761 catggaggag tgcc
//
LOCUS       DBMDTI010000269         2767 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8115_length_2767_cov_4.828582, whole genome shotgun sequence.
ACCESSION   DBMDTI010000269 DBMDTI010000000
VERSION     DBMDTI010000269.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8115_length_2767_cov_4.828582"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="ACZPB6_09465"
     CDS             complement(<1..1355)
                     /locus_tag="ACZPB6_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1184727.1"
                     /translation="MFNTIRYLLLPVLNASGPVFIWLAAALLIPFTLSVSKQDGAADS
                     FLTCFLITLATGVVLMLLTRRWRRELTSRHGFLLVTLIWTSVPLFGTIPFIIERPDMS
                     FAQLYFEMMSCLTTTGATVFTGLDKLPVSLNGWRCFLSWIGGMGLIVLSVAILPLLGV
                     GGSQIIKAETTGPLKEHRLTPRIADTAKALYIIYLGVSVACAISYHLAGMEWEDAIMH
                     MMTTVSLSGIGTHDASFAYFNSPAVDAVAIVFMLISGFNFSLHFMVWRRRNFLIYLQD
                     AEAKAWIATAALMSALTIIVLADAHVYETSAETVRHALFSVVSVITTTGYATVDYASW
                     PLGLPVLLLVAAAFSTCAGSTGGGIKMVRLLIILGMIRREMTKLLYPKAVVPLTLNKT
                     PIDEKVCIAVLAYFMVWLTSFVIGAAVLLLAGLPPGDAISAAWAMLSNIGPGLGGVGP
                     TG"
     gene            complement(1370..>2767)
                     /gene="trkA"
                     /locus_tag="ACZPB6_09470"
     CDS             complement(1370..>2767)
                     /gene="trkA"
                     /locus_tag="ACZPB6_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR1184728.1"
                     /translation="IVGAGRIGSSVAESLVSEANDITVVDQDAERIGSLQSRFDLRGV
                     VGNATSPQVLTDAGAADTDMLIAVTSSDETNLVVTMLCARIFNIPTRIARVRNNELRD
                     YPRILGEEGFEATSIIWPEQALVSYLIKLIDFPEAIQVQEFGEGLASLIAVRAKSGSP
                     LVGRPIRELFTHLPQVPARIVAIFRRNRRLEVNASTIVEAGDEVICLCDTRHARRVMA
                     ELRHRERPVHSIMIAGKPSFTLNLARMLKTGENRPGGNFDIRILENDRNVVKRLSREL
                     GDAAMLIEGDFTSEDVLDAAGIETCDLFIAVSENDENNILGALLAKKLGAGKTIALVN
                     RKAYGDLMQGSQIDITLSTTTAALGELIRHVRRGDVAAAYSLRRGAAEALEIVAHGDR
                     RSSKVVGRRIGSISLPEGCAIGALIRGNDENALVLMGDKDTVIEAEDHVIVFVPSKKL
                     IPKIERLFTVNVGFF"
BASE COUNT          509 a          805 c          957 g          496 t
ORIGIN      
        1 cccgtcgggc cgacgccgcc gaggcccggg ccgatgttcg aaagcatggc ccatgcggcg
       61 ctgatggcgt ccccgggggg gagtcccgcc agaagcagaa cggctgcgcc gatcacgaag
      121 gacgtgagcc agaccatgaa gtaggccagc acggcgatgc agactttttc gtcgatcggc
      181 gtcttgttga gcgtgagcgg aaccaccgcc ttcggataaa gcagcttcgt catttccctt
      241 cggatcatgc cgaggatgat cagcaggcgc accatcttga tgccgccgcc cgtcgagcct
      301 gcgcacgtcg agaacgctgc ggcgaccagc aggaggacgg gcaggccgag cggccaggag
      361 gcgtagtcga ccgttgcgta gcccgtcgtc gtgatgacgg agaccactga gaagagcgcg
      421 tggcgcacgg tttcggccga cgtttcgtag acgtgcgcgt cggcgagcac gatgatcgtg
      481 agggccgaca tcagggcggc cgttgcaatc caggccttcg cctccgcgtc ctgcaggtag
      541 atcaggaagt tcctgcgcct ccataccatg aagtggaggg agaagttgaa gccgctgatc
      601 agcatgaaga cgatggcgac cgcatcgacg gcgggactgt tgaagtatgc gaagcttgcg
      661 tcatgcgtgc cgatgcccga gaggctgacg gtcgtcatca tgtgcatgat ggcgtcttcc
      721 cattccatgc ctgccagatg gtaggagatc gcgcaggcga cggagacgcc gagatagatg
      781 atgtagagcg ccttggccgt gtcggcgatg cggggcgtca ggcggtgctc cttgaggggg
      841 cccgtcgtct cggccttgat gatctgcgag ccgcccacgc cgagcagcgg gagaatggcc
      901 acggacagca cgatgaggcc catgccgccg atccacgaga ggaagcagcg ccagccgttc
      961 agtgatacgg gaagcttgtc gagtcccgtg aagaccgttg cgcccgtcgt cgtgaggcag
     1021 gacatcatct cgaagtagag ctgtgcaaag ctcatgtcgg ggcgttcgat gatgaaggga
     1081 atcgtgccga agagcggcac ggaggtccat atcagcgtca cgagaagaaa gccgtgcctt
     1141 gaagtcagct cccgccgcca gcgacgggtc agaagcatca ggaccacgcc cgtggcgagc
     1201 gtgatgagaa aacacgtcag aaagctgtcc gccgcgccgt cctgctttga gacggagagc
     1261 gtgaacggaa tgagcagcgc cgcggcgagc cagatgaaga cgggcccaga ggcattgagc
     1321 accggcagca gcagatagcg gatcgtgttg aacatggcgt caggcctcct tagaagaagc
     1381 cgacattgac cgtgaagagt cgttcgatct tcgggatgag cttcttggag ggcacgaaga
     1441 cgatcacgtg gtcctccgcc tcgatgacgg tgtccttgtc gcccatcagt acgagcgcgt
     1501 tttcatcgtt gccgcggatc agggctccga tggcgcagcc ttcgggcagc gagatgctgc
     1561 cgatgcgcct gccgacgacc ttggagcttc tgcggtcgcc gtgggcgacg atttcgagcg
     1621 cttcggccgc gccgcgtctc aggctgtagg cggccgccac gtcgccgcgg cgcacgtggc
     1681 ggatcagctc gccgagcgcg gcggtggtgg tcgacagcgt gatgtcgatc tggctgccct
     1741 gcatgaggtc gccgtaggcc ttgcggttga cgagcgcaat ggtcttgcct gcgccgagct
     1801 tcttggccag aagggcgccg aggatgttgt tctcgtcgtt ttcggacacg gcaatgaaca
     1861 ggtcgcacgt ctcgatgcct gcggcgtcaa gcacgtcctc gctggtgaag tcgccctcga
     1921 tcagcatggc ggcatcgccg agctcgcggg agaggcgctt cacgacgttg cggtcattct
     1981 ccagaatcct gatgtcgaag ttgccgcccg gacggttttc gccggtcttg agcatgcgcg
     2041 cgagattgag ggtgaagctg ggctttcccg caatcatgat gctgtgcacg gggcgctcgc
     2101 gatggcgcag ctccgccatg acgcgcctgg cgtgtctggt gtcgcacaga cagatgacct
     2161 cgtcgcccgc ctcgacaatg gtggaggcgt tcacctccag acggcggttg cggcggaaga
     2221 tggccacgat gcgtgcggga acctgaggca gatgcgtgaa gagttcacgg atcgggcggc
     2281 ccaccagcgg actgcccgac ttggcgcgca cggcgatgag gctcgcaagc ccttctccga
     2341 attcctgaac ctgaatggct tcgggaaagt caatgagctt gatgagatag ctcacgagcg
     2401 cctgctcggg ccagatgatg gacgttgctt caaagccttc ctcgcccaaa atgcggggat
     2461 agtctcgaag ctcgttgttg cggacgcggg cgatgcgggt cggaatgttg aagatgcggg
     2521 cgcagagcat cgttacgacg aggttcgtct cgtcgctcga ggtgacggcg atcagcatgt
     2581 ccgtatcggc cgcgcctgca tcggtcagca cctgcgggct ggtggcgttg cccacgacgc
     2641 cgcgcaggtc gaatctcgac tgcagggatc cgatgcgctc ggcgtcctgg tcaacgacgg
     2701 tgatgtcgtt ggcttcggag accagactct cggcgaccga gctgccgatg cggcctgcgc
     2761 ccacaat
//
LOCUS       DBMDTI010000270         2761 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8137_length_2761_cov_3.097368, whole genome shotgun sequence.
ACCESSION   DBMDTI010000270 DBMDTI010000000
VERSION     DBMDTI010000270.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8137_length_2761_cov_3.097368"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="ACZPB6_09475"
     CDS             complement(<1..240)
                     /locus_tag="ACZPB6_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1184729.1"
                     /translation="MNARALTAFACAIAALASLGSVPAAAGERLDNIVKNNTLRVGTP
                     GDYRPFAMKEDSGYAGHDIDVVKAMAEVYGWKVEFV"
     gene            complement(375..599)
                     /locus_tag="ACZPB6_09480"
     CDS             complement(375..599)
                     /locus_tag="ACZPB6_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HIR1184730.1"
                     /translation="MSSDIRIKLSDFDAELLANIAEREGRTPEAVVLEALHELFSERL
                     EEKSFLISKDEFEACLAILSEPARKAAAAD"
     gene            complement(847..2640)
                     /locus_tag="ACZPB6_09485"
     CDS             complement(847..2640)
                     /locus_tag="ACZPB6_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184731.1"
                     /translation="MKKTLIAFAVAAAFASAASAASFKAGTYTATAPGIHGDVTVEVT
                     FTADKLADIKVTKQSETVGIGSKAVELLPGRMVAAQSPAVDGVTGATITTEAIRKAVS
                     DCVKQAGVDPANLVPLAVKKTGGQKTLETDMVVVGGGGSGMSATIRGRMNGMNVILVE
                     KMPYIGGAAAISGGQVVAQGSKLQKAFGSTEDSPESMIKDFMANGHNLNDPAKISLYA
                     NNVGATIDWLHEKVGVKFIPNDLPFLAEYSHRRALEFEGGAKTMAQHLREVIAGNGAQ
                     VLYSTRVEKLLQDKDGRVIGVEATDDNGVKYTIKSKATLLTTGGFGNNKDMLVEPIKS
                     TLYYGPVSSTGDGHRMAMELGAKTQLMQYGKRYPNGIEVAPSKAKSTIYANVGAFDQA
                     GILVNIDGKRFVNEKASNRHILDPMLQNANGQGYVFMDQKSWEGFYKRLPETGVSHED
                     ADKYLAQDGKTAPLFVKGATVEEVAAKAGINAAALKATVARYNGFVKAKKDADFDRPV
                     QYMKAEISAEGPYYIVEQRPRFATTMGGLCTNDKLNVITKDGKLIPGLYAAGELVGGV
                     MGDDSPAGANVGWALTSGCVAADAIKEAVAK"
BASE COUNT          452 a          752 c          995 g          562 t
ORIGIN      
        1 gacgaactcg accttccagc cgtagacctc ggccatcgcc ttgacgacgt caatgtcgtg
       61 gcccgcgtag ccggaatcct ccttcatcgc gaatggcctg tagtcgcccg gcgtgccgac
      121 gcgaagcgtg ttgtttttaa cgatgttgtc gagtctttcg cccgctgcgg cgggaacgga
      181 gccaagggag gcgagggctg cgatggcgca ggcgaaggcc gtgagtgcgc gtgcgttcat
      241 gattgttttg agacgaaaaa aaagacagtc cgcatccgct gccggctcgg acgaggatgg
      301 atcggggctg cgagtggccg gcgcagccgg gatgcggcgg gaaggtgcga atcctgagtg
      361 tcgtcagtca ggcgtcagtc tgcggcggcc gccttcctgg cgggctcgga gagaatggcg
      421 agacaggcct cgaactcgtc cttggagatg aggaaggact tttcctcaag cctttcggag
      481 aagagttcgt gcagggcctc gagcacgacc gcttcgggcg tcctgccttc gcgttcggcg
      541 atgttggcaa gcagctcggc gtcgaagtcg ctcaatttga ttctgatatc ggaagacatg
      601 tccgttcctc caaccaaaca tttccacttt gcattgtatg taggcgtaat gcgttttgga
      661 aggcagtagg tgttcgaacc taggcagaag tgcgcaggcg cattgagcgc gcagggcggc
      721 gcgcggggcg ctgacgaaaa taggggcccg aagccgattg gagagcttcg ggcccggaag
      781 tcgggagccg ccgttgcggg gcggccccaa tggggaagag aagtggttgg gaaggttcgt
      841 cgacccttac ttggcgacgg cttccttgat ggcgtccgca gcgacacagc cggaggtgag
      901 ggcccagccc acgttggcgc cggccgggga gtcgtcgccc atcacgccgc cgacgagttc
      961 acccgcagcg tagagacccg ggatgagctt gccgtccttg gtgatgacgt tgagcttgtc
     1021 gttcgtgcag aggccgccca tcgtggtggc gaagcgggga cgctgctcga cgatgtagta
     1081 cgggccttcg gcggagattt cggccttcat gtactggacc ggacggtcga agtcggcgtc
     1141 cttcttggcc ttgacgaagc cgttgtagcg ggcgacggtg gccttgaggg cggcggcgtt
     1201 gatgccggcc ttggcggcga cttcttcaac cgtggcgccc ttcacgaaga gcggagcggt
     1261 cttgccgtcc tgggcgaggt acttgtcggc atcctcatgg gagacgcccg tttcaggcag
     1321 gcgcttgtag aagccttccc aggacttctg gtccatgaag acgtagcctt ggccgttggc
     1381 gttctggagc atcggatcga ggatgtggcg gttcgaggcc ttttcgttca cgaagcgctt
     1441 gccgtcgatg ttgacgagaa tgccggcttg gtcgaaggcg ccgacgttgg cgtagatggt
     1501 ggacttggcc ttgctcggcg cgacttcaat gccgttcggg tagcgcttgc cgtattgcat
     1561 gagctgcgtc ttggcgccga gttccatggc catgcggtgg ccgtcgcccg tggaggagac
     1621 gggaccgtag tagagcgtgg acttgatcgg ctcgacgagc atgtccttgt tgttgccgaa
     1681 gccgcccgtg gtgagcagcg tggccttgga cttgatcgtg tacttgacgc cgttgtcgtc
     1741 cgtggcttca acgccgatca cgcggccgtc cttgtcctga aggagctttt cgacgcgggt
     1801 gctgtagagc acctgggcgc cgttgccggc gatgacttca cgaaggtgct gggccatcgt
     1861 cttagcgccg ccttcgaatt caagggcgcg gcggtgggag tactcggcga ggaacggcag
     1921 gtcgttcggg atgaacttca cgcctacctt ttcatgcagc cagtcgatcg tggcgccgac
     1981 attgttggcg tagagcgaga tcttggccgg gtcgttgagg ttgtggccgt tggccatgaa
     2041 gtccttgatc atggactcgg gagagtcttc cgtggagccg aaggccttct ggagcttgga
     2101 gccctgggcg acgacctgac cgccggagat ggcggcggcg ccgccgatgt acggcatctt
     2161 ctcgacgagg atgacgttca tgccgttcat gcggccgcgg atcgtggcgg acatgccgga
     2221 accgccgccg cccacgacga ccatgtcggt ttcaagcgtc ttctggccgc cggtcttctt
     2281 gacggcgagc ggaacgaggt tggcgggatc gacgccggcc tgcttgacgc agtcggagac
     2341 ggctttgcgg atggcttcgg tggtgatggt ggcgcccgtc acgccgtcaa cggcgggaga
     2401 ctgggcggcg accatgcggc cgggaaggag ttcaacggcc ttggagccga tgcccacggt
     2461 ctcggactgc ttcgtgacct tgatgtcggc gagcttgtcg gcggtgaacg tcacttcgac
     2521 ggtgacgtcg ccgtggatgc cgggggcggt ggccgtgtag gtgccagcct tgaagctcgc
     2581 ggcggaggcg gcggacgcaa aggccgcggc aactgcaaag gcgatcagag tcttcttcat
     2641 aataaattcc tgaacttgtg atgagtcgta ggttgatgaa aaaggagaac gtttgtcctt
     2701 cggatcgaag gagacgggcc cgcgcccccg ggggatggag gcgggcgcag gcccgcagga
     2761 g
//
LOCUS       DBMDTI010000271         2749 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8180_length_2749_cov_1.950529, whole genome shotgun sequence.
ACCESSION   DBMDTI010000271 DBMDTI010000000
VERSION     DBMDTI010000271.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8180_length_2749_cov_1.950529"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="ACZPB6_09490"
     CDS             <1..185
                     /locus_tag="ACZPB6_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HIR1184732.1"
                     /translation="PEARFLMDSPVTALLRWLASQFEIDRGKGVTDSHPLVRPPVGPD
                     VHEIKTDNAKNKEPAK"
     gene            182..1060
                     /locus_tag="ACZPB6_09495"
     CDS             182..1060
                     /locus_tag="ACZPB6_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1184733.1"
                     /translation="MNAITCDDLVFRRGDRTVLNRIGFELAPGRLLGLIGHNGAGKST
                     LIKLILGLLKPASGRLEVLGGAPGASPLDVGYLPENVSFYDAMTVEEHLRYFAGLKGV
                     ARPRIDELVEELGLGVVLRQRLGQCSKGQRQRLGLAQALLTRPRLLMLDEPTVGLDPA
                     ASSLMYRELSALRDAGCAVVVCTHELALVEPYLDQVLLLATGECRGSGTLEDLRAKAA
                     LPALITNVGSGDALADPELAPYVRGTRLAVPEGDVARVVRRLTEAHGVYDFQLRKADL
                     GMIFAHFVLDVPVEEL"
     gene            1060..1911
                     /locus_tag="ACZPB6_09500"
     CDS             1060..1911
                     /locus_tag="ACZPB6_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1184734.1"
                     /translation="MTSSTSSAIGTGSRIGALATVSIVAGKEFRDCLRSRWLVFGSTL
                     FAILGLAVFFGTAAIGGTLQYQPLPSVMNSLLSLTVFLLPLLALLLSYDAFVGEAESG
                     TLLLMLTYPLSRLQWLAGKALGQGAALLLVLVLGFAVLPLIQAFLPVPYGMGELLSSL
                     CVLVLSGWALGLLFMLAAYWVSLSVRHKAQALALLLVVWFVAVLLYDLGLLVVTVAGA
                     DVLGRGTLTALMLANPASVFRLLNQSVIGVLSFEVPSAAVLSGILAAWIAVLFAICAR
                     TLAVRRL"
     gene            2098..>2749
                     /gene="ccsA"
                     /locus_tag="ACZPB6_09505"
     CDS             2098..>2749
                     /gene="ccsA"
                     /locus_tag="ACZPB6_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013725.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR1184735.1"
                     /translation="MIPELGHFLLIIAAMTALLAPAVVFYGIRRGDDFYAGFSRPASL
                     LTALMLTLSTAALVWAFLTDDFSVAYVANHSNTKLAPAYKAAALWGSHEGSMLLWIWL
                     MAVGTAGAGFVRCADRLFTARLQGLMLAVLAGFCAFLLFTSNPFLRNLPMVPPDGRDL
                     NPILQDIGMILHPPVLFMGYAGLALMFAAMCALLLSGRWSKTAIDFTTRLSIVTWGF"
BASE COUNT          367 a         1000 c          824 g          558 t
ORIGIN      
        1 atcccgaggc ccgcttcctg atggacagcc ccgtgacggc gctcctcagg tggctcgcca
       61 gccagtttga aatcgaccgc ggcaagggcg ttaccgactc ccacccgctc gtgcgcccgc
      121 ccgtcggccc cgacgtgcac gaaatcaaga cagacaacgc gaagaacaag gagcctgcca
      181 aatgaacgcc atcacatgcg acgacctcgt cttccgccgc ggcgacagga ccgtcctcaa
      241 caggatcggc ttcgagctcg ctccgggaag gcttctcggc ctcatcggtc acaacggcgc
      301 aggcaagtcg accctcatca agctgatcct cggccttctc aagcccgctt cgggcaggct
      361 cgaggtgctc ggcggtgcgc cgggcgcctc gccccttgac gtgggctacc tgcccgagaa
      421 cgtgagcttc tacgacgcca tgaccgtcga ggagcatctc cgctacttcg cgggcctcaa
      481 gggcgtcgcg cgcccgcgca tcgacgagct cgtcgaggag ctcgggctcg gtgtcgtgct
      541 gcgccagcgc ctcggccagt gctcgaaggg ccagcgccag cgcctcggcc ttgcacaggc
      601 gcttctcacc cgcccgcgcc ttctgatgct cgacgagccg accgtgggcc tcgaccccgc
      661 cgcctcgtcc ctcatgtacc gcgagctctc cgcgcttcgc gacgcgggct gcgccgtcgt
      721 cgtctgcacg cacgagcttg cgctcgtcga accctatctc gaccaggtgc tgcttctggc
      781 cacaggcgag tgccgcggca gcggcacgct tgaggacctg cgcgccaagg ccgcgctgcc
      841 cgcgctcatc acgaacgtcg gcagcggcga cgcgcttgcc gaccccgagc tcgcgcccta
      901 cgtgcgcggc acgcgtctcg ccgttcccga gggcgacgtc gcccgcgtcg tcaggcgcct
      961 caccgaggcg cacggcgtct acgacttcca gcttcgcaag gcggatctgg gcatgatctt
     1021 cgcgcacttc gttctggacg tgcccgtgga ggaactgtaa tgacttctag cacttcatcc
     1081 gccatcggca ccggctcccg catcggcgcc ctcgccaccg tctccatcgt ggcgggcaag
     1141 gagttccgcg actgcttgag aagccgctgg ctcgtcttcg gaagcacgct cttcgcgatt
     1201 ctgggtctgg ccgtcttctt cggcacggcc gccatcggcg gcacgcttca gtaccagccg
     1261 ctgccttccg tgatgaactc gctcctgagc ctcacggtct tcctgctgcc gcttctcgcg
     1321 ctgctcctct cctacgacgc cttcgtgggc gaggcggagt cgggcacgct tcttctgatg
     1381 ctcacctatc cgctctcgcg ccttcagtgg ctcgcaggca aggcgctcgg gcagggtgcg
     1441 gcgctccttc tcgttctcgt gctgggcttt gcggtcctgc cgctcatcca ggccttcctg
     1501 ccggtgccct acggcatggg tgagttgctt tcgtctctct gcgtgctcgt tctgagcggg
     1561 tgggcgctcg gactcctctt catgctcgcc gcctactggg tgagcctttc ggttcgtcac
     1621 aaggcgcagg ccctcgcgct tctgctcgtc gtctggttcg tcgccgtgct tctctacgac
     1681 ctcggtcttc tcgtcgtgac ggttgcgggt gcggacgtgc tcggccgcgg aacattgacg
     1741 gccctcatgc tcgccaatcc cgcgagcgtc ttcaggcttc tcaaccagtc cgtgatcggt
     1801 gtcctctcct tcgaggtgcc gtccgctgcg gtcctctcgg gcattctcgc cgcatggatt
     1861 gcggtgctct tcgcgatctg cgcaaggacg ctcgccgtcc gcaggctata agctccgcag
     1921 aaggtcgtcc gggtcacgaa caggaatcgc cggtcgtgcc gctgcagttt gtacggcagg
     1981 cacgacgggc gtttcgcagg ccctcggacc ggaagccgtc ttaagcctca gtgcaggttt
     2041 gcgcgttaaa ttccagtctg catacatccg accagaccaa ccatcaggac atccaaagtg
     2101 atccccgaac tcggccactt cctgctcatc atcgccgcca tgacggcgct ccttgcaccc
     2161 gccgtggtct tctacggcat tcgaaggggc gacgacttct atgcgggctt cagccgccct
     2221 gcgtctcttc tgacggcgct catgctcacg ctttcgacgg ccgcgctcgt ctgggccttc
     2281 ctgaccgacg atttctccgt cgcctacgtc gcaaatcact ccaacaccaa gctcgcgccc
     2341 gcctacaagg ccgcggccct ctggggctcg cacgaaggct cgatgctcct ctggatctgg
     2401 ctcatggcgg tcggcacggc cggggcgggc ttcgtccggt gcgccgacag gctcttcacg
     2461 gcgcggcttc aggggctgat gcttgcggtg ctcgcgggct tctgcgcgtt cctgctcttc
     2521 acgtcgaatc cgttcctgcg caacctgccg atggtgccgc ccgacggtcg tgacctcaat
     2581 cccatcctgc aggacatcgg catgatcctg catccgcccg tgctcttcat gggctatgcg
     2641 ggcctggcgc tgatgttcgc ggccatgtgc gcgctgctgc tttcgggccg ctggtcgaag
     2701 acggccatcg acttcacgac gcgtctttcg atcgtcacct ggggcttcc
//
LOCUS       DBMDTI010000272         2747 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8196_length_2747_cov_1.923658, whole genome shotgun sequence.
ACCESSION   DBMDTI010000272 DBMDTI010000000
VERSION     DBMDTI010000272.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8196_length_2747_cov_1.923658"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /gene="htpG"
                     /locus_tag="ACZPB6_09510"
     CDS             <1..1362
                     /gene="htpG"
                     /locus_tag="ACZPB6_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HIR1184736.1"
                     /translation="DEKEFLGQWRLRDAITKYSDHISTPVFLWEKKEPVKEDDKDVVE
                     AEVTGSWEQVNDARALWTLPPKDVTEDQYKAFYKHLTHDYGDPLCWAHNRVEGELEYT
                     SLLYTPSEAPWDLYSREKQHGLKLFVQRVFIMDDAEQFLPNYLRFIRGLVDTADLPLN
                     VSRELLQESRVTQKLKRALTKRALDMLGKLAKDDQEKYAVFWKNFGAVMKEGPVEDYA
                     NRDAVLKLLRFASTKAGTPEENVSLADYVARMPEKQKHIYYLTAGNFETAVSSPYLET
                     FRRKGVEVLLMTQRIDEWMMQGMTEFEGHEFIPVTASDLKLGELADSEEEKKHEEVVR
                     ESSGLVERIKQALGDRVEGVRVSSRLVDSPSCLVADADKRLTPQMRRMLEAAGQQVPE
                     DKVTLEINPSHPLLKKAEAEKDQAGFEKWCELMLDQAALADQGTVKDPARFVKLMNEL
                     LLK"
     gene            complement(1600..2394)
                     /locus_tag="ACZPB6_09515"
     CDS             complement(1600..2394)
                     /locus_tag="ACZPB6_09515"
                     /inference="COORDINATES: protein motif:HMM:NF015412.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase
                     [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase domain-containing
                     protein"
                     /protein_id="HIR1184737.1"
                     /translation="MKRAAIIGCGALGTILVTNFARAEGWTLTGVMARTKAHAEALAA
                     NAGCRAATTLEELLADKPDLVVEIAGIGAAKAYGEAVLEAGCDFVLVSAGALADADWK
                     VRMTETALRTGRRIHVASGAIGGFDVLRTAALMGVEEATFESTKSPKSLSGAPGLMGV
                     ELPADRETLAFEGGVEEAVRGFPKNINVAAATASAADAPNVRVRLVARPGLTVNTHHI
                     DVKAGEMHCELFFHSAYDPKNPRSSTSTAWSVLALLKNLESPISYF"
BASE COUNT          502 a          928 c          862 g          455 t
ORIGIN      
        1 gacgagaagg agttcctcgg ccagtggcgc ctgcgcgacg cgatcacgaa gtactccgac
       61 cacatctcca cgcccgtctt cctctgggag aagaaggaac ccgtgaagga ggacgacaag
      121 gacgtcgtcg aggctgaagt gacgggctcc tgggagcagg tcaacgacgc ccgcgccctc
      181 tggacgcttc ccccgaagga cgtgacggaa gaccagtaca aggccttcta taaacacctc
      241 acgcacgact atggcgatcc cctctgctgg gcccacaacc gcgtcgaggg cgagctcgag
      301 tacacgtccc ttctctacac gccgtccgaa gccccgtggg acctctactc gcgtgaaaag
      361 cagcacggcc tcaagctctt cgtgcagcgc gtcttcatca tggacgacgc cgagcagttc
      421 ctgccgaact acctgcgctt catccgcggc ctcgtcgaca ccgccgacct gccgctcaac
      481 gtctcccgcg agcttctcca ggaaagccgc gtgacgcaga agctcaagcg cgccctcacg
      541 aagcgtgcgc tcgacatgct cggcaagctc gcgaaggacg atcaggaaaa gtatgccgtc
      601 ttctggaaga acttcggcgc cgtcatgaag gaaggccccg tcgaggacta cgccaaccgc
      661 gacgccgtcc tcaagctcct gcgctttgcc tccaccaagg ccggcacgcc cgaggagaac
      721 gtaagcctcg ccgactacgt tgcccgcatg cccgagaagc agaagcacat ctactacctc
      781 accgccggca acttcgagac cgccgtgagc tccccgtacc tcgagacctt ccgccgcaag
      841 ggcgtcgaag tcctcctgat gacgcagcgc atcgacgagt ggatgatgca gggcatgacc
      901 gagttcgaag gtcatgagtt catccccgtg accgcctccg acctcaagct cggcgagctc
      961 gccgacagcg aagaggaaaa gaagcacgag gaagtcgttc gcgaatcctc cggtctcgtc
     1021 gaacgcatca agcaggcgct cggcgaccgc gtcgaaggcg tgcgcgtctc ctcccgcctc
     1081 gtcgacagcc cgagctgcct cgtggccgac gccgacaagc gcctcacgcc gcagatgcgc
     1141 cgcatgctcg aggccgcagg ccagcaggtc cccgaggaca aggtgaccct cgagatcaac
     1201 ccgtcccacc cgctcctcaa gaaggccgag gccgaaaagg accaggcggg cttcgagaag
     1261 tggtgcgaac tgatgctcga tcaggccgcc ctcgccgacc agggcacggt caaggacccc
     1321 gccaggttcg tgaagctcat gaacgagctg ctgctgaaat aagctccgcc cttacggacg
     1381 cagtctgaca tcagccaaaa aggcgcctcc cctccgcggg ccggcgcctt ttttcacggc
     1441 ctcatctcat atgtcgactt gtttcaccat caattcgaga ccctgaatcg atgcggcgca
     1501 cgctcccatg caaaagacgg cgtccatttg cgtgccctca agggcgcttc gccccgaaag
     1561 cgctgcggcg cagggcggcg ggcgcgtttt ggagtcgcgt cagaagtagg agatcgggct
     1621 ctcgaggttc ttgagaagcg cgagcacgct ccaggcggtg gacgtgctag agcgcgggtt
     1681 cttcggatcg taggcggagt ggaagaagag ctcgcagtgc atctcgcccg ccttcacgtc
     1741 gatgtgatgg gtgttgacgg tgaggccggg tctggcgacg aggcgcaccc tgacattcgg
     1801 agcgtcggcg gcggaagccg tggcggccgc gacgttgatg ttcttcggga agcccctgac
     1861 ggcctcctcg acgccgccct cgaaggcgag cgtctcgcga tcggcgggga gctccacgcc
     1921 catgaggccg ggcgcgcccg agaggctctt cggggacttg gtgctctcga aggtcgcctc
     1981 ctcgacgccc atgagggcgg ccgtgcgcag cacgtcgaag ccgccgatgg cgcccgaggc
     2041 gacgtggatg cggcggcccg tgcgcagcgc cgtttcggtc atgcgaacct tccagtcggc
     2101 gtcggcgagc gcgcctgcgg agacgagcac gaagtcacag cctgcttcga gcacggcttc
     2161 gccataggcc tttgcggcgc cgatgcccgc gatctcgacg acgaggtcgg gcttgtcggc
     2221 gaggagctcc tcaagcgtcg ttgctgcgcg gcagcccgcg ttggcggcga gcgcctcggc
     2281 gtgcgccttc gttcgggcca tgacgcccgt gagcgtccag ccctcggccc tggcaaagtt
     2341 cgtgacaaga atggtgccca aggcaccgca gccgatgatg gcggctcgct tcatgatggc
     2401 tctcccatgt ttgataattc tcaggaaccg acggcgactc aatgaaatcc gcctcggtcc
     2461 ggtgcagaga gtctagaccg tcggaggggc ttttcgccgc cgcgttttcc cgcacggttc
     2521 gtggatgtgc gaagggccgc ccgtcgtcca tctccaacag gcggccctcg cattgtcgtc
     2581 agcaggcgga ggcttcaaac cccttctcgg ctcccgccta ctttgccgca gccttggccg
     2641 cgggcctggc gggcttgacg cccggccaac cggtttcgcc cgcgtagaaa tcggcgagcg
     2701 cgttgatttc atcgtcggtg agcttctgcg cgaagaagcg catttcc
//
LOCUS       DBMDTI010000273         2744 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8216_length_2744_cov_2.598941, whole genome shotgun sequence.
ACCESSION   DBMDTI010000273 DBMDTI010000000
VERSION     DBMDTI010000273.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8216_length_2744_cov_2.598941"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1808)
                     /locus_tag="ACZPB6_09520"
     CDS             complement(<1..1808)
                     /locus_tag="ACZPB6_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR1184738.1"
                     /translation="MDTFILAALALLGALAVIDLFVGVSNDAVNFLNSAVGSRTAPFW
                     VIMTVASLGVLLGATFSSGMMEVARTGVLVPEHFTFKEVIIVFTAVMVCDVLLLNTFN
                     SLGLPTSTTVSIVFELFGGATALACYKVWSSGAPIADLGLYLNSAKALAMIMAILVSV
                     VVAFFAGLFVQYLLRLIFSFNYERYYKLFGGIYGGVSLTAVFYFLVIKGAKGASFMQP
                     EWISWIGANTTELLLGCFAILTVLFQALIMALRINIFPIIILAGTFSLAFAFAGNDLV
                     NFIGVPLAALSSYEIWSAVEGADPTTFTMEGLRHNQGTPTYFLLASGLVMVLTLWFSK
                     KAHRVIRTSISLASSARGGKEQFGSSLPARVVVRSAIRFNNLAHQIIPASVIKSLATR
                     FEKKKLAPGEVELPFDEIRASVNLVLAAALIASATSLKLPLSTTYVTFMVAMGSSLAD
                     GAWDRESAVYRISGVLTVISGWFMTAFTAFTACGLICMLFISWGEPLQLIMMVLALIV
                     VIRTNFFVKEEDETPDHERIDRGDKASIRDLLTESVNDNLNATLTLYAQGLQAFLDED
                     DQKLRELKGDAAQLFDFITMRRGEYYNMALEGGGSRS"
     gene            complement(2042..>2744)
                     /locus_tag="ACZPB6_09525"
     CDS             complement(2042..>2744)
                     /locus_tag="ACZPB6_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1184739.1"
                     /translation="GAALAVITLLILFFIHEPPRKEQKKAAAAREKTSLLKIPVVQRM
                     LLCAGVVQLTILLQQPVMPMYIGELQGSMDRIVFVTGIVFSVVGVSGVIASPLWGVIG
                     QKWGYRPALYTALAGTACFGMIQAIPDTLVPFAVWRFIGGLTFAGIFPAINAVLTMST
                     PPEDRGRIFGLSYAAQQVGSVIGPLVGGGMAMYLPLKSVIFLGGFILLPLVIFLWYMR
                     PKNEENSHGSPAQLR"
BASE COUNT          572 a          755 c          988 g          429 t
ORIGIN      
        1 gagcgcgagc cgccgccctc aagggccatg ttgtagtact ctccgcggcg catcgtgatg
       61 aagtcgaaga gctgtgcggc gtcgcccttg agctcgcgca gcttctgatc gtcctcgtca
      121 aggaaggcct gaaggccctg cgcgtagagc gtgagcgtgg cgttgaggtt gtcgttgacg
      181 ctttcggtga gcaggtcgcg gatgctggcc ttgtcgccgc ggtcgatgcg ctcgtggtcg
      241 ggcgtctcgt cctcttcctt gacgaagaag ttcgtgcgga tgacgacgat gagcgcgagc
      301 accatcatga tgagctgaag cggctcgccc cagctgatga agagcatgca gatgaggccg
      361 caggcggtga aggccgtgaa ggcggtcatg aaccagccgg agatcacggt gagcacgccc
      421 gagatgcggt acacggcgga ctcgcggtcc caggcgccgt cggcgagcga cgaacccatg
      481 gcgaccatga aggtcacgta cgtggtcgag agcgggagct tcaggctcgt ggccgaggcg
      541 atgagcgcgg cggcgagcac gaggttcacg ctggcgcgga tctcgtcgaa cggcagctcg
      601 acctcgcccg gcgcgagctt ctttttctcg aagcgcgtgg ctaggctctt gatgacggag
      661 gccggaatga tctgatgggc gaggttgttg aagcggatcg cggagcgcac gacgacgcgg
      721 gcgggaagcg aggaaccgaa ctgctccttg ccgccgcggg cggacgaggc gaggctgatc
      781 gaggtgcgga tgacgcggtg ggccttcttg gagaaccaga gcgtgagcac catcacgagg
      841 ccggaggcaa gcaggaaata ggtcggcgtg ccttggttgt ggcgcaggcc ctccatcgtg
      901 aaggtcgtcg gatcggcgcc ctcgaccgcg gaccagatct cataggagga gagcgcggcg
      961 agcggcacgc cgatgaagtt cacgaggtcg ttgcccgcga aggcgaaggc gagcgagaac
     1021 gttccggcga gaatgatgat cgggaagatg ttgatgcgca gcgccatgat gagcgcctgg
     1081 aagagcacgg tcaggatggc gaagcagccg agcaggagtt cggtcgtgtt ggcgccgatc
     1141 caggagatcc actcgggctg catgaaggac gcgcccttgg cgcccttgat cacgaggaag
     1201 tagaagacgg ccgtgagcga gacgccgccg tagatgccgc cgaagagctt gtagtagcgc
     1261 tcgtagttga aggagaaaat cagacgcagc agatactgca cgaagaggcc cgcaaagaag
     1321 gccacgacga cggatacgag aatcgccatg atcatggcga gcgccttggc ggagttgagg
     1381 tagagcccca gatccgcaat gggcgcgccg ctcgaccaga ccttgtagca ggcgagcgcg
     1441 gtggcgccgc cgaagagttc gaacacgatc gagacggtcg tggaggtggg aaggccgagg
     1501 gagttgaagg tgttgagcag gaggacgtcg cagaccatca cggccgtgaa gacaatgatc
     1561 acttccttga aggtgaagtg ctcgggcacg agaacgcctg tgcgggcgac ctccatcatg
     1621 cctgaggaga aggtggcgcc gagcaggacg cccagactgg ccaccgtcat gatcacccag
     1681 aagggtgccg tgcgggagcc gacggcggaa ttgaggaagt tgacggcgtc attggagacg
     1741 ccgacgaaga ggtcgatgac ggcaagcgcg cccaaaagcg ccaacgcagc aagtatgaat
     1801 gtatccattc gatttgtatc tgcccgaacg gcccggccag tgccgacgct ccgagggaaa
     1861 cgacttcacg gcgtgacagt tcctgacaat ttgcaatcag tattaatggt tcgattgtat
     1921 cgaacctcag gccgtatttg gggacccgtc tgctgaaaac actcaaaatg acagcaccgc
     1981 gcggagaagc cgcgagctcg taccagcttc gaattactcc caaaagcgat gccctgcggg
     2041 actagcgcag ctgggcggga ctgccgtgcg agttctcctc gttcttcgga cgcatgtacc
     2101 agaggaagat gacaagcggc aggagaatga aaccgccgag gaagatgacg gacttgagcg
     2161 gcaggtacat ggccatgccg ccgcccacga gcgggccgat cacgctgccg acctgctgtg
     2221 ccgcgtagga gaggccgaag atgcggccgc ggtcctcggg cggcgtgctc atcgtgagca
     2281 cggcgttgat ggcggggaag atgcccgcga aggtgaggcc gccgatgaag cgccagacgg
     2341 cgaacggcac gagcgtatcg ggaatcgcct ggatcatgcc gaagcaggcc gtgcccgcaa
     2401 gcgccgtgta gagcgcgggg cggtatcccc acttctggcc gatgacgccc cagaggggcg
     2461 aggcgatcac gcccgaaacg ccgaccacgg agaagacgat gccggtcacg aagacgatgc
     2521 ggtccatgct gccctggagc tcgccgatgt acatcggcat gacgggctgc tgcaggagga
     2581 tcgtgagttg cacgacgccc gcgcacagga gcatgcgctg cacgacggga atcttgagga
     2641 ggctggtctt ctcgcgcgcg gcggccgcct tcttctgctc ctttctcgga ggctcgtgga
     2701 tgaagaagag gatgaggagc gtgatgacgg caagtgcggc gccg
//
LOCUS       DBMDTI010000274         2738 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8234_length_2738_cov_2.226773, whole genome shotgun sequence.
ACCESSION   DBMDTI010000274 DBMDTI010000000
VERSION     DBMDTI010000274.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8234_length_2738_cov_2.226773"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="ACZPB6_09530"
     CDS             complement(<1..190)
                     /locus_tag="ACZPB6_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012958.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR1184740.1"
                     /translation="MGHSFGGLFALSTMASRPELFDAWVAADPSLWWDGGVLIRMLED
                     KPAAGHSGAFVYAGFGSAL"
     gene            189..410
                     /locus_tag="ACZPB6_09535"
     CDS             189..410
                     /locus_tag="ACZPB6_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013219.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HIR1184741.1"
                     /translation="MRTCVTTRPSSRLFMTYRHGTLIAGSVLIGIVVLADGQFAVEHL
                     TAFSVPVSVFITIMGGVYFLYLILRSKGL"
     gene            423..1178
                     /locus_tag="ACZPB6_09540"
     CDS             423..1178
                     /locus_tag="ACZPB6_09540"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HIR1184742.1"
                     /translation="MLSIQTLTKLYDGIPVVDDVSLEIPKGKVTSLIGPNGAGKSTVL
                     GIAARLLAAEHGDVLFDGRSIKSWNTRALARRLAVLTQTNTVQMKVTVRELVGFGRFP
                     HSGTRLTDDDRRRVDEAIRYMGLEAFADRFIDEMSGGQRQRAFIAMVLAQDTDVIFLD
                     EPTNNLDIRHSLSMMRLLRRLADELGKTVILVLHEINLASFYSDRICAFKDGRLAFSG
                     TPAEVMTEERLRAVYGVDFRIVEFDGRPMALYH"
     gene            1253..2605
                     /locus_tag="ACZPB6_09545"
     CDS             1253..2605
                     /locus_tag="ACZPB6_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013648.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1184743.1"
                     /translation="MKTMLSRTLAALLVAVAALPAVAVQAAETLTVQTFNEKNAPVEV
                     VIPKDPKRVAVLDYAVLDTMDAWGLGNRVTALPKSTALPWLAKYKNDSKIVNTGTPKE
                     VDFEALMASEPDVIFVSGRLVKKIPELRRIAPVIYQKTDRELGSLEQTKAKLVQLGGI
                     FGKTAEAAGRAFDERAAAIRTASAGRTAVVGMVTSSHFNMLGLKQVQGLPHRQRVRLQ
                     KHRPRRQHRPRQRVLVRASRRAQSRLHLRLRLRLRHLPSGRQARVGRDDQPARREDRC
                     REERPHRPPHLVRLVPRRGRPARHRHDVRGHRKGARHRQITPFHPPIRPPFSAASHPH
                     WVRFRLSFTGEPFFSTFFIGKPPDRVLAKKGDNNQPYRAITAGAIHQLLRARADESQV
                     SSFTPHDLRRTFATRLLESGADINTVRQAMGHSSVVTTRRYDKRDEHLVEEATRKVAL
                     "
BASE COUNT          527 a          998 c          752 g          461 t
ORIGIN      
        1 tcagggcaga gccgaagccc gcgtagacga aggcgccgga gtgccccgca gccggtttgt
       61 cctccagcat tcgaatgagc acgccgccgt cccaccagag cgacggatcg gcggccaccc
      121 aggcgtcaaa gagctcgggg cgtgaagcca tcgtcgagag tgcaaagaga cctccgaagg
      181 agtgtcccat gagaacgtgc gtcacgacgc ggccttcgag ccgcctcttc atgacctacc
      241 gccacggcac gctcatcgca ggcagcgtcc tcatcggcat cgtcgtgctc gcggacggac
      301 agttcgccgt cgagcacctc acagccttca gcgtccccgt gagcgtcttc atcacgatca
      361 tgggcggcgt ctacttcctc tatctcatcc ttcgcagcaa aggactctga tccgccatga
      421 ccatgctctc catccaaacg ctcaccaagc tctacgacgg cattcccgtg gtcgacgacg
      481 tctcgctcga gatcccgaag ggaaaggtca cgtcgctcat cggccccaac ggcgcgggca
      541 agtccaccgt gctcggcatc gccgcacgcc ttctcgccgc cgagcacggc gacgtgctct
      601 tcgacggacg ctcgatcaaa agctggaaca cccgcgcgct cgccagacgc ctcgcggttc
      661 tcacccagac gaataccgtc cagatgaagg tcacggtccg cgaactcgtt gggttcggac
      721 gctttccgca ctcgggcacg cggctcaccg acgacgaccg gcgccgcgtc gacgaggcca
      781 tccgctacat gggcctcgag gctttcgccg accgcttcat cgacgaaatg tcgggcggcc
      841 agcgccagcg cgccttcatt gccatggtcc tcgcccagga caccgacgtc atcttcctcg
      901 acgagcctac gaacaacctc gacatccgcc actcgctctc gatgatgagg ctcctgagac
      961 gcctcgccga cgagctcggc aagaccgtca tcctcgtcct gcacgagatc aaccttgcgt
     1021 ccttctactc cgaccgcatc tgcgccttca aggacggcag gctcgccttc tcgggcacgc
     1081 ccgccgaggt catgaccgag gagcgcctca gggcggtcta cggagtcgac ttcaggatcg
     1141 tcgagttcga cggccgcccc atggcgctct accactgagt ccctgcacgg gctgtcaacc
     1201 atttcatcca cgcacacctt tcaacaaccg tctccataca aacaggaaaa ccatgaagac
     1261 catgctctcc cgcacgctcg ccgcgctcct cgtcgcggtc gccgccctgc ccgccgtcgc
     1321 cgtccaggcg gccgaaaccc tcacggtgca gaccttcaac gagaagaacg cgcccgtcga
     1381 ggtcgtcatt ccgaaggacc cgaagcgcgt cgccgtcctt gactacgccg tcctcgacac
     1441 catggacgcc tggggcctcg gcaaccgcgt gaccgccctg ccgaagtcca cggcccttcc
     1501 ctggctcgcg aagtacaaga acgactcgaa gatcgtcaac accggcacgc ccaaggaagt
     1561 cgacttcgag gccctcatgg cgagcgaacc cgacgtcatc ttcgtgagcg gccgcctcgt
     1621 caagaagatc cccgaactgc gccgcatcgc gcccgtcatc tatcagaaga ccgaccgcga
     1681 gctcggcagc ctcgagcaga cgaaagccaa gctcgtgcag ctcggcggca tcttcggcaa
     1741 gaccgccgag gccgcgggcc gcgccttcga cgagcgcgcc gccgccatcc gcaccgcctc
     1801 cgccggccga accgccgtcg tcggcatggt cacctcgagc cacttcaaca tgctcgggct
     1861 caagcaagtc caagggctcc ctcatcggca acgagttcgg cttcaaaaac atcgcccacg
     1921 gcgccaacac cgaccacggc agcgagtcct cgttcgagct tctcgtcgag ctcaatcccg
     1981 acttcatctt cgtctgcgac tgcgactccg ccatctcccg tccgggcgcc aggctcgcgt
     2041 aggacgtgat gaccaacccg ctcgtcgaga ggaccgatgc cgcgaagaac ggccgcatcg
     2101 tccacctcac ctcgtccgcc tggtacctcg ccgagggcgg cctgcgcgcc accgacatga
     2161 tgttcgcgga catcgaaaag gcgctcggca tcgccaaata acgccctttc atccgccaat
     2221 acgtccgcct ttttcagcgg catcccatcc tcattgggtt cgttttcggc tctccttcac
     2281 gggagagccg tttttttcga ccttttttat cggcaagcca cccgataggg tgcttgcgaa
     2341 gaagggcgac aacaaccagc cataccgggc gattacggcc ggcgccatcc atcagctgct
     2401 gcgcgccagg gcggacgaat ctcaggtttc gtccttcacg ccgcatgatc tgcgccgaac
     2461 ctttgcgacg cggctccttg agtccggcgc cgacatcaac accgtgcggc aggcgatggg
     2521 acattcctcc gtcgtgacga cccggcgcta cgacaagcgt gatgagcatc tggtggagga
     2581 agcaacgagg aaggtggcgc tttgagcgaa gcgggcgctc caagcttgac tctggcaaaa
     2641 tatcggcagc agggatcgct tgcaaacgcc attctgaaat ccaccccact ctccggcaag
     2701 gctcacgaaa ttttcgatga cgagccttcg gcatcgga
//
LOCUS       DBMDTI010000275         2721 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8291_length_2721_cov_2.338550, whole genome shotgun sequence.
ACCESSION   DBMDTI010000275 DBMDTI010000000
VERSION     DBMDTI010000275.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8291_length_2721_cov_2.338550"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="ACZPB6_09550"
     CDS             <1..723
                     /locus_tag="ACZPB6_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbI"
                     /protein_id="HIR1184744.1"
                     /translation="EGVDAKVNTISKINGQYLIGSKYSFWALDESLKPVVSAAYDPWY
                     SANVLDLVAITPYKDDAFILMGSNKSLLRARLTPNGDDVKNWGNFTAGRTQVEHVGGL
                     GRARVGTERAKFSYVHSAASDGRYVYTATVPDNRNKKTFVISKALMSDWMLSGEFVPS
                     ADLKEGRTLGELYVTGMVYENGSLFAVSKNHNVLVEIDVAAEKVVNAWSIPAELTDVR
                     GLVKTGDTFEVVDTNRIVTLKK"
     gene            1240..>2721
                     /locus_tag="ACZPB6_09555"
     CDS             1240..>2721
                     /locus_tag="ACZPB6_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR1184745.1"
                     /translation="MNGLTLMAVVIVCLWLGYHFYGRWLEKTWGIDPNAKTPAALRND
                     GKDYQPASRLSVFAHQFTSITGAGPVTGPIIAAMFGWLPALLWIIIGGIFFGAVQDFT
                     ALYASVRNGGKSMGMIIEQYVGRFGRQLFLAFCWLFTLLVIAAFADMIAATFNGFTKT
                     GEQNMPNAAAASISMIYIVGAVIFGLFQKYVKPSSGLQLAVGITMMIAMLAMGIAWPI
                     YADADTWRVVVFLYAFAASVMPMWILKQPRDFLSMFLLFGMIIAGVVGVFVTNPTINM
                     PAFVGWEVKGLDLFPILFVTIACGAISGFHSLVSSGTSSKMIANETDMRPVGYGAMLV
                     ECVLGALALTIVCAAASTTGQLPSGTPFQLFSGAVAGFLTNTFGLPADISACVLTMCV
                     SALAMTTVDAVARIGRMSFQELFATTDGREKGPVAKFLSNTWVSTLLTLGGGWFLCLA
                     GYMNIWPLFGSANQLLGALVLTALAVFLQSTGRKGWMLWVPMTV"
BASE COUNT          469 a          998 c          708 g          546 t
ORIGIN      
        1 gaaggcgtgg acgccaaggt caacacgatc tcgaagatca acggccagta cctcatcggc
       61 tccaagtaca gcttctgggc ccttgacgag tccctcaagc cggtcgtctc cgccgcttat
      121 gatccgtggt actccgccaa cgtcctcgac ctcgtggcca tcacgccgta caaggacgac
      181 gccttcatcc tcatgggctc caacaagtcg ctcctgcgcg ctcgtctcac cccgaacggc
      241 gacgacgtca agaactgggg caacttcacg gccggccgca cccaggtcga gcatgtcggc
      301 ggtcttggcc gcgcccgcgt cggcactgaa cgtgccaagt tctcctacgt gcactccgcc
      361 gcctccgacg gccgctacgt ctacaccgcc acggtgcccg acaaccgcaa caagaagacc
      421 ttcgtgatct cgaaggccct catgtccgac tggatgctct ccggtgaatt cgtcccgtcc
      481 gccgacctca aggaaggccg cacgctcggc gaactctacg tgaccggcat ggtctacgag
      541 aacggcagcc tcttcgccgt ctccaagaac cacaacgtcc tcgtcgaaat cgacgtggcc
      601 gctgaaaagg tcgtcaacgc ctggtcgatc ccggccgagc tcaccgacgt ccgcggcctc
      661 gtgaagacgg gcgatacgtt cgaagtcgtc gacacgaacc gcatcgtcac gctcaagaag
      721 taattcttgc cagccgtcag tgcttgatcg ggccggctcc ctgcagggag ccggcccgat
      781 tttttccttt cctcccccgc gcaccatctg cgtgcgcacg caccatcaac gcctcgcgcc
      841 gctccccttc acggtgcaga aatccccttc tcgccctcaa gaaaaactca gggcgaacat
      901 cctccgatag atttccgcaa tatccgcctc ccgcgcccct tcgggcgcac gtccgatgcg
      961 ttttcgagcg acgaccgccc gaaaacgacc tcccgacgag cgttttcatt tgaccgtcac
     1021 ttataggcga cactttcggg ataagccgat gttccaaggc agggcctgct ttcctcaagc
     1081 cgcctaggct gaacatccat gaggctttct ccctaagtcc tatgccttat aaggcaaaga
     1141 gcctagtcac ccctgtgcga caggcattta gagtagcaaa tgaaggaggc cctctcgacc
     1201 tcccgacata caagaacaac ttttcaggat gatctacaga tgaacggtct cactctcatg
     1261 gccgtcgtga tcgtctgcct ctggctcggc taccacttct acggtcgctg gctcgaaaag
     1321 acatggggca tcgatccgaa cgccaaaaca cccgccgcgc ttcgcaacga cggcaaggac
     1381 tatcagcccg cctcccgcct ctccgttttc gcgcaccagt tcacgtccat tacgggcgcc
     1441 ggtcccgtca cgggtcccat cattgccgcc atgttcggct ggcttcccgc gcttttgtgg
     1501 atcatcattg gcggcatctt cttcggcgcc gtccaggact tcacggctct ctacgcctcc
     1561 gtcaggaacg gcggcaagtc catgggcatg atcatcgagc aatacgtcgg ccgcttcggc
     1621 cgccagctct tcctcgcctt ctgctggctc ttcacgcttc tcgtcatcgc cgccttcgcc
     1681 gacatgatcg ccgcgacgtt caacggcttc acgaagacgg gcgaacagaa catgcccaac
     1741 gctgccgccg cctcgatctc catgatctac atcgtcggcg ccgtgatctt cggcctcttc
     1801 cagaagtacg tcaagccctc gagcggcctg cagctcgccg tcggcatcac catgatgatc
     1861 gccatgctcg ccatgggcat cgcctggccg atctacgccg atgccgacac ctggcgcgtc
     1921 gtggtcttcc tctacgcctt cgccgcttcc gtgatgccga tgtggatcct caagcagccg
     1981 cgtgacttcc tctcgatgtt cctgctcttc ggcatgatca tcgccggcgt cgtcggcgtg
     2041 ttcgtgacga acccgaccat caacatgccc gccttcgtgg gctgggaagt gaaggggctc
     2101 gatctcttcc cgatcctctt cgtgacgatc gcctgcggcg ccatctcggg cttccacagc
     2161 ctggtgtcct ccggcacgtc ctccaagatg atcgccaatg aaaccgacat gcgtcccgtc
     2221 ggctacggcg ccatgctcgt cgaatgcgtc ctcggcgccc ttgccctgac catcgtctgc
     2281 gccgccgcct ccacgacggg ccagctgccc tcgggcaccc cgttccagct cttctcgggc
     2341 gctgttgccg gcttcctgac gaacaccttc gggctccccg ccgacatctc cgcctgcgtt
     2401 ctgacgatgt gcgtctccgc cctcgccatg accacggtcg acgccgtggc ccgcatcggc
     2461 cgcatgagct tccaggagct cttcgccacg accgacggca gggaaaaggg ccccgttgcg
     2521 aagttcctca gcaacacctg ggtctccacg ctgctcacgc tgggcggcgg ctggttcctc
     2581 tgcctcgccg gctacatgaa catctggccg ctcttcggct ccgccaacca gctcctcggc
     2641 gccctcgtcc tcacagcgct cgccgtgttc ctccagagta cgggccgcaa gggctggatg
     2701 ctctgggtcc cgatgaccgt c
//
LOCUS       DBMDTI010000276         2715 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8320_length_2715_cov_2.735654, whole genome shotgun sequence.
ACCESSION   DBMDTI010000276 DBMDTI010000000
VERSION     DBMDTI010000276.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8320_length_2715_cov_2.735654"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(63..548)
                     /locus_tag="ACZPB6_09560"
     CDS             complement(63..548)
                     /locus_tag="ACZPB6_09560"
                     /inference="COORDINATES: protein motif:HMM:NF025084.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HIR1184746.1"
                     /translation="MTGFRVPGPGRIHVFCEGETEVLYLQDFCRLIGLSGKKAVISVC
                     PVKNPARAIEWICRQVPGVSKASAASPECWLVFDRDRHEGFHEAFELVKQHPCIHLAA
                     TKPCFEYWLLLHFDDFQDDLPLAQGWYVAKRQVQGKQVAPDRSVRMTMEFPALYPASG
                     I"
     gene            complement(751..1137)
                     /locus_tag="ACZPB6_09565"
     CDS             complement(751..1137)
                     /locus_tag="ACZPB6_09565"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HIR1184747.1"
                     /translation="MKTIEVVAAIIEHEGRILATQRGYGDFKDGWEFPGGKMEPGETP
                     EAAIVREIEEELKVTIAPKTLVTTVECDYPKFHLTMHCFLSSIVKGEICLVEHEAAKW
                     LSPDELDSVDWLPADVEVVEKLKTLL"
     gene            complement(1207..>2715)
                     /locus_tag="ACZPB6_09570"
     CDS             complement(1207..>2715)
                     /locus_tag="ACZPB6_09570"
                     /inference="COORDINATES: protein motif:HMM:NF023335.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HIR1184748.1"
                     /translation="RGLMTVALTGEDEQSLREEAIYRLSYGVGDNRLDYIFTVDIFAE
                     GVDIPEVNQVIMLRPTQSAIVFVQQLGRGLRKAPDKEFVVILDFIGNYQSNFLIPVAL
                     ADDRSYNKDNMRRALAVDLRQIPGASSVHFDPIVRKRIYESIDRASVSDTKLIRTSYE
                     QLKAKLGRIPELLDFDRFGSIDAVKFFEKFGSYYAFLEKYEKSFTTRLSTRAAGMLAF
                     LSVKAGAGRRVSEAIVIRDLLAGRPDLETGLREGLARYGIEPSAAHLSSVFGFLSNRF
                     VKTADEAKQRAGMVFVEYDAALGWRMAAELREELGRSAEFRTMLAELAGFMVSRYEAR
                     FSKRYAGTDLVLYEKYTYEDVCRLLNWPTNMNAQNIGGYFYEKTTKTMPVFVNYHKAE
                     GAIAYEDRFVSPSHIVALSKTKRRTTSADADHIYKRTPEDKDNRILLFVRRNKEDKGE
                     AKAFYFLGEVEAQGEPVPVTLPATGDRAFEIDYRLDVPVRSDIYAYVTMAED"
BASE COUNT          520 a          841 c          807 g          547 t
ORIGIN      
        1 aatagagagt gccatgacta aatttaagca atctgcggat gcgcaatata tcaaagatgg
       61 ggttaaattc cacttgcagg ataaagagca ggaaactcca tcgtcatccg gacgcttctg
      121 tccggagcca cctgcttccc ctgcacctgc cgcttcgcaa cgtaccaacc ctgcgccaga
      181 ggaagatcat cctgaaaatc atcgaagtga agaagcagcc agtactcgaa gcacggcttc
      241 gtggccgcaa gatgtatgca gggatgctgc ttcacaagct cgaacgcctc gtgaaagcct
      301 tcatggcggt cgcgatcaaa gaccagccag cactcgggag aggcggcgga cgccttcgaa
      361 acgccgggca cttgccggca tatccactcg atcgcccgtg cgggattctt cacgggacag
      421 acggagatga ccgccttttt tccggaaagg ccgatcagcc tgcagaagtc ctgcaaatag
      481 agcacctcag tctcgccttc gcagaagaca tggatcctgc cgggccccgg aacccggaac
      541 ccggtcatgc gggagatttg acatcgtcat gagaatctcc ttcaggcagt cgcaaatgac
      601 cggctcacgc ctgatcgtac tcgagcgcag cgaaaaagtc cctctcccca aagcactctt
      661 ttgtgacgaa tgttcacgcg gcgcatccgc cgtcgaaaaa ggcccgcctc gaaaccgaag
      721 cgggcccttc ttctcccttc gagacctttg tcagagcagc gtcttgagct tctcgacgac
      781 ctccacgtcg gcaggcagcc agtcgacgct gtcgagctcg tcgggcgaga gccacttcgc
      841 cgcctcatgt tcgacgagac agatctctcc cttcacgatg gacgaaagga agcagtgcat
      901 cgtgaggtga aacttcggat agtcgcactc gaccgtcgtc acgagggtct tgggtgcaat
      961 cgtcaccttg agctcctcct cgatctcgcg cacgatggcg gcctcgggcg tttcgccggg
     1021 ctccatcttg ccgccgggaa actcccaccc gtccttgaag tcgccgtacc cgcgctgggt
     1081 ggcgagaatc ctgccttcat gctcgatgat ggctgcgacc acttcgatcg tcttcattca
     1141 tactccttgc tgttttgaat tgagcttttg tcggcttcgt gccgccgtgc cgcgcgcctt
     1201 cggattctaa tcctcggcca tcgtcacgta ggcgtagatg tcggaacgca cgggcacgtc
     1261 gagtctgtaa tcgatctcga aggcgcggtc gcccgttgcg ggaagcgtca cgggcacggg
     1321 ctcgccctgc gcctcaacct ccccgaggaa gtagaaggcc ttcgcctcac ccttgtcctc
     1381 cttgttgcgg cgcacgaaga gaaggatcct gttgtccttg tcctcgggcg tgcgcttata
     1441 gatgtggtcc gcgtcggccg acgttgtcct gcgcttcgtc ttcgagagcg ccacgatgtg
     1501 cgacggcgag acgaagcggt cctcgtaagc gatcgcgcct tcggccttgt ggtagttgac
     1561 gaagacgggc atcgtcttcg tcgtcttctc gtagaagtac ccgccgatgt tctgggcgtt
     1621 catgttggtc ggccagttga gcagcctgca gacgtcctcg tacgtatatt tttcgtagag
     1681 aacgaggtcg gtgcccgcgt agcgcttcga gaacctggcc tcgtagcgcg agaccatgaa
     1741 gcccgcgagc tccgcgagca tcgtgcggaa ctccgcgctt cggccgagct cctcgcgaag
     1801 ttccgcggcc atgcgccatc cgagcgcggc gtcgtactcg acaaagacca tcccggcgcg
     1861 ctgcttcgct tcgtcggcgg tcttcacgaa gcggttcgaa agaaagccga agacgctcga
     1921 gagatgcgcg gccgagggct cgatcccgta gcgggcgagt ccctcgcgaa gtcccgtctc
     1981 aaggtccggc ctgcccgcga gcaggtccct gatgacaatc gcctcggaaa cgcgcctgcc
     2041 cgcgcccgcc ttcacggaca ggaaggcgag catgcctgcg gcgcgcgtcg agagcctagt
     2101 cgtgaaggac ttctcgtact tttcgagaaa ggcgtagtag gagccgaact tctcgaagaa
     2161 cttgacggca tcgatcgagc cgaacctgtc gaagtcgaga agctccggaa tgcggccgag
     2221 cttggccttg agctgctcgt aactcgtgcg gatgagcttt gtgtcgctca cgctcgcgcg
     2281 gtcgatcgac tcgtagatgc gcttgcgcac gatcggatcg aagtgcacgc tcgaggctcc
     2341 cggaatctgc ctcagatcga cggcgagcgc gcggcgcatg ttgtccttgt tgtacgagcg
     2401 gtcgtcggcg agcgccaccg gaatcagaaa gttcgactgg tagttcccga tgaagtcgag
     2461 aatgaccacg aattccttgt caggtgcctt gcgaaggcct cggccgagct gctgcacgaa
     2521 gacgatcgcg ctctgcgtcg gacgcagcat gatgacctgg ttgacttcgg gaatgtcgac
     2581 gccctcggcg aagatgtcga ccgtgaagat gtagtcgagc ctgttgtcgc ccacgccgta
     2641 cgagaggcgg tagatcgcct cctcgcgcag gctctgctca tcctcgcccg tgagcgcgac
     2701 cgtcatgagg ccgcg
//
LOCUS       DBMDTI010000277         2702 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8384_length_2702_cov_3.854287, whole genome shotgun sequence.
ACCESSION   DBMDTI010000277 DBMDTI010000000
VERSION     DBMDTI010000277.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8384_length_2702_cov_3.854287"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1902
                     /gene="dnaK"
                     /locus_tag="ACZPB6_09575"
     CDS             <1..1902
                     /gene="dnaK"
                     /locus_tag="ACZPB6_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR1184749.1"
                     /translation="GIDLGTTNSAVAVMEGDNIKVIENSEGARTTPSIVAYMDNGEVL
                     VGAPAKRQAVTNPKNTLFAVKRLIGRRFDDAEVQKDINLMPFTIMRADNGDAWVEAQG
                     KKLAPQQVSAEILRKMKQTAEDYLGEAVTEAVITVPAYFNDSQRQATKDAGRIAGLEV
                     KRIINEPTAAALAFGMDKGGSSDRKIAVYDLGGGTFDISIIDLANIDGDKQFEVLSTN
                     GDTFLGGEDFDQRLVDHLINEFRKDTGVDLSKDVLALQRLKDAAEKAKIELSSRQETE
                     INLPYITADATGPKHLNVTVTRAKFEALVEDLVQRTIEPVRKALRDAGTSTSDITDVI
                     LVGGQTRMPRVQAVVKEFFGKDPRKDVNPDEAVAIGAAIQGSVLTGERRDVLLLDVTP
                     LTLGIETLGGVMTPMIKRNTTIPTKHSQVFSTAEDNQPAVTIKVYQGEREMAASNKLL
                     GEFNLEGIPASPRGLPQIEVTFDIDANGILHVAAKDKATGKENTITIKASSGLSEDEI
                     ERMVQDAEANKEEDHRRAELAQARNQADAAVSQVQKTLKEFGDKVDGADRAACEDAIR
                     TVEEKVKGEDKAAIDKAVENLMAAAQKIGEKMNKAAAQQQAAPEGNAAGSKPADDDVV
                     DADFTDVKK"
     gene            2063..>2702
                     /locus_tag="ACZPB6_09580"
     CDS             2063..>2702
                     /locus_tag="ACZPB6_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR1184750.1"
                     /translation="MSDRDYYEVLGVGKDASADDIKKAYRRMAMKYHPDRNPGDKVAE
                     EKFKEIGEAYAVLSDDQKRAAYDRYGKAGVDPSAAGGPGGFGGFGNMNGADFQSAFGD
                     IFSDIFGGGGMRGGRAAREQGIRGNDVRFELEISLEDAAMGRTMEIRVPAWDECQSCH
                     GTGCKPGTSRKTCPTCQGHGAVRVSNGLFQVHQTCPKCHGSGQIISDPCPNCQ"
BASE COUNT          589 a          905 c          810 g          398 t
ORIGIN      
        1 ggtattgacc ttggcaccac caattcggcc gttgccgtga tggaaggcga caacatcaag
       61 gttattgaaa acagcgaagg cgcccgcacg accccgtcca tcgttgccta catggacaac
      121 ggtgaagtgc tcgtcggcgc cccggccaag cgccaggctg tgaccaatcc gaagaacacg
      181 ctctttgccg tgaagcgtct catcggccgc cgcttcgacg acgccgaagt ccagaaggac
      241 atcaacctga tgccgttcac gatcatgcgc gccgacaacg gcgacgcttg ggtcgaagct
      301 cagggcaaga agctcgctcc gcagcaggtc agcgccgaaa tccttcgcaa gatgaagcag
      361 accgccgaag actacctcgg cgaagctgtc acggaagccg tcatcacggt tcccgcctac
      421 ttcaacgact cccagcgtca ggccacgaag gacgccggcc gcatcgccgg tcttgaagtg
      481 aagcgcatca tcaacgaacc gaccgctgcc gcgctcgcct tcggcatgga caagggcggc
      541 agcagcgacc gcaagatcgc cgtgtacgac cttggcggcg gcacgttcga catctcgatc
      601 atcgacctcg ccaacattga cggcgacaag cagtttgaag tgctctccac gaacggcgac
      661 acgttcctcg gcggcgaaga cttcgaccag cgcctcgtcg accacctgat caacgagttc
      721 cgcaaggaca cgggcgtcga cctctccaag gacgttctcg ccctgcagcg cctgaaggac
      781 gccgctgaaa aggccaagat cgagctctcg agtcgtcagg aaaccgaaat caacctgccg
      841 tacatcacgg ctgacgccac gggtccgaag cacctcaacg tcacggtcac gcgcgccaag
      901 ttcgaagccc tcgtcgagga cctcgtccag cgcaccatcg agccggtccg caaggccctg
      961 cgcgatgccg gcacgtccac gtccgacatc accgacgtca tcctcgtcgg cggtcagacc
     1021 cgcatgccgc gcgttcaggc cgtcgtgaag gaattcttcg gcaaggatcc gcgcaaggac
     1081 gtcaaccccg acgaggccgt cgccatcggc gccgccatcc agggctccgt cctcaccggc
     1141 gaacgccgcg acgtgctcct gctcgacgtc accccgctta cgctcggcat tgagacgctc
     1201 ggcggcgtga tgaccccgat gatcaagcgc aacacgacga ttccgacgaa gcacagccag
     1261 gtgttctcga ccgctgaaga caaccagccg gccgtgacca tcaaggtcta ccagggcgaa
     1321 cgcgaaatgg ctgcctccaa caagctcctc ggcgagttca accttgaagg cattccggcg
     1381 tctccgcgcg gcttgccgca gatcgaagtc acgttcgaca tcgacgccaa cggcattctt
     1441 cacgtggccg ccaaggacaa ggccaccggc aaggaaaata ccatcacgat caaggccagc
     1501 tccgggctga gcgaagacga gatcgaacgc atggtgcagg acgccgaagc caacaaggaa
     1561 gaggaccacc gccgcgccga gctcgctcag gcccgcaacc aggccgacgc cgccgtcagc
     1621 caggtccaga agaccctgaa ggaattcggc gacaaggtgg acggtgccga ccgcgccgcc
     1681 tgcgaagacg ccatccgcac ggttgaagag aaagtcaagg gtgaggacaa ggccgccatc
     1741 gacaaggccg tcgaaaacct gatggccgcc gctcagaaga tcggcgaaaa gatgaacaag
     1801 gccgccgccc agcagcaggc cgctccggaa ggcaacgccg ccggcagcaa gcccgccgac
     1861 gatgatgtgg tcgacgccga cttcacggac gtcaagaagt aatccgtgag tgacggcaat
     1921 ccgggagccc cggctcccag gctgattcgg tcttgagtca gcgcatcaaa cgaagggtcg
     1981 gttcgctcac gcgatgccgg cccttttcga gttttgaaaa aatcaaaaag aagacggggt
     2041 agctccccgc agggattggg taatgtctga tcgcgattat tacgaggtgc tcggcgtcgg
     2101 caaggacgct tccgccgacg acatcaagaa ggcctatcgt cgcatggcca tgaagtacca
     2161 tccggaccgc aatccgggtg acaaggtcgc cgaagagaag ttcaaggaaa tcggtgaagc
     2221 ctacgccgtg ctttccgacg accagaagcg cgccgcctac gaccgctacg gcaaggcggg
     2281 cgtggatccc agcgctgcgg gcggtcccgg cggcttcggt ggctttggca acatgaacgg
     2341 cgccgacttc cagagcgcct tcggcgacat cttcagcgac atcttcggcg gtggcggcat
     2401 gcgcggcggc cgtgccgccc gcgagcaggg catccgcggc aacgacgtgc gcttcgagct
     2461 ggaaatctcg cttgaggacg ccgccatggg ccgcacgatg gagatccgcg tgcccgcctg
     2521 ggacgagtgc cagtcctgcc acggtacggg ctgcaagccc ggcacgtcta ggaagacctg
     2581 tccgacctgt cagggccatg gcgccgtgcg cgtctccaac ggtctcttcc aggtccacca
     2641 gacctgcccg aagtgccatg gttccggcca gatcatctcc gatccgtgcc cgaactgcca
     2701 gg
//
LOCUS       DBMDTI010000278         2693 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8425_length_2693_cov_3.149306, whole genome shotgun sequence.
ACCESSION   DBMDTI010000278 DBMDTI010000000
VERSION     DBMDTI010000278.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8425_length_2693_cov_3.149306"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /gene="pcnB"
                     /locus_tag="ACZPB6_09585"
     CDS             complement(<1..1335)
                     /gene="pcnB"
                     /locus_tag="ACZPB6_09585"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0043633 - polyadenylation-dependent RNA
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HIR1184751.1"
                     /translation="MFGRIVNRVRDMFAPRSVVVKNPRVISADEHRIDPALVSAEARA
                     TCEALKRRGFQAYIVGGAVRDLMLGVTPKDFDVATDATPEQVKRCQRRAVIIGRRFKL
                     VHVIFGREIIECSTFRALEGAGQRKDATGRVVSDNVFGEMWEDAARRDFTINALYYDP
                     QTQELFDYHRGFEDLAAKRLRMIGDPAERYREDPVRMMRAVRIAAKLGFTIEPATEAP
                     IPKMAKLLENVPSARLVDEVLKLLTSGHAVACLSKLRQEGLHKALLPLLDVIVSEPDG
                     EEFLMLALKRTDERIAVGKKISPSFLFGTLLWPQVVKRWRYNEDEKGMSRMAALHAAC
                     VDVLATQCQQLNIQRRYQADIHDLWMLQARFERRTGKTAYALVSHPRYRAAYDFMLLR
                     SLLGHVPESLVQWWDAFALSDEETRAGMIAEAQREARMTGDAARSHRRRAASA"
     gene            complement(1336..2022)
                     /locus_tag="ACZPB6_09590"
     CDS             complement(1336..2022)
                     /locus_tag="ACZPB6_09590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1184752.1"
                     /translation="MKFAVFDLDHTLLPVDSGENWSRWLVRAAGMDVALMDARIEAYA
                     EAYRKGAFSADEFMGFQAGLLAACRRQDLEKWCTAYIDACVRPHLRPEALDLVESRRK
                     AGFEPVLASGTHAFVTGAIAPLFGIRHLVAARPEEDAFGEFTGRIAGSHSYGEGKLVL
                     LREFLESLGAADDGLRLEAYSDSINDLPMLAFAAERGEAFAVNPDGMLRSEAARRGWR
                     IMDIFKKDRA"
     gene            complement(2040..>2693)
                     /locus_tag="ACZPB6_09595"
     CDS             complement(2040..>2693)
                     /locus_tag="ACZPB6_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdaA/DnaA family protein"
                     /protein_id="HIR1184753.1"
                     /translation="YLLDLQERDKPTLDNFVVGANGEAVSVLREVAAGCGPTFVYLYG
                     PKGAGLTHLLRSLVPEEGFRVPMFDPKVPLYVVDDVDELDPGWALQLLALQNAVRGTP
                     GMHLVCAGRVPAAQLPLPEVVRSRLAWGLCYAIRPLDDEERLAELHRQAARRGIELTP
                     DILHWMGANLPRDMRTLTCVLDEADRLGLQKQRRVTLPLLREAVTSLDERQHARQAY"
BASE COUNT          531 a          860 c          845 g          457 t
ORIGIN      
        1 ggcggacgct gcgcggcgtc gatggctgcg ggcggcgtcc cccgtcatgc gggcctcgcg
       61 ctgagcctct gcgatcatgc ctgcacgagt ttcctcgtcg gagagcgcaa aagcgtccca
      121 ccactgaacg agagactcgg gcacatgacc gagcagcgaa cgaagcagca tgaagtcata
      181 ggcagcacga tagcggggat gggagacgag cgcataggca gtcttgcccg tgcggcgctc
      241 gaacctcgcc tgaagcatcc acagatcgtg gatgtccgcc tgatagcgtc gctggatgtt
      301 cagctgctgg cactgggtgg caagcacgtc gacgcaggcc gcatgaaggg cggccatgcg
      361 gctcatgccc ttttcatcct cgttgtagcg ccagcgcttg acgacctgcg gccagagcag
      421 cgtgccaaaa aggaaggagg gcgaaatctt cttgccgacg gcgatgcgct cgtcggtgcg
      481 cttcagggcg agcatcagga actcctcgcc gtcaggctcg gagacgatca cgtccagcaa
      541 cggcagaagc gccttgtgaa gtccctcctg acgcaacttc gagaggcagg ccacggcatg
      601 accgctggtc agaagcttga gtacctcgtc gacaagacgg gcggagggca cgttttcgag
      661 aagcttcgcc attttgggaa tcggcgcttc agtggcgggc tcgatcgtga agccgagctt
      721 cgccgcgatg cgcacggcgc gcatcattcg cacggggtct tccctgtagc gctcggctgg
      781 atcaccgatc atgcgaaggc gctttgcggc gagatcttca aagccgcggt gatagtcgaa
      841 gagctcctgc gtctggggat cgtagtacag cgcattgatc gtgaagtcgc gcctggccgc
      901 gtcctcccac atctcgccga agacgttgtc ggagacgacg cgacccgtgg cgtccttgcg
      961 ctggcccgcg ccctcgagcg cacggaaggt cgagcactcg atgatctcgc gcccgaaaat
     1021 cacatgtacg agcttgaagc gacggccgat gatcacggcg cggcgctggc agcgcttcac
     1081 ctgttcgggc gtcgcatcgg tcgccacgtc gaagtccttg ggcgtgacgc cgagcatcag
     1141 gtcgcgaacg gcaccgccca cgatgtaggc ctggaagccg cgacgcttga gcgcctcgca
     1201 ggtcgcccgc gcctcagccg acaccagggc cggatcgatg cggtgctcat ctgcggaaat
     1261 cacgcgggga ttcttgacga caacggaacg gggggcgaac atgtccctca cccggttgac
     1321 aatacggcca aacatttacg ccctgtcctt tttgaaaata tccatgattc gccaacctct
     1381 gcgggcggct tccgaccgaa gcatgccgtc cggattgacg gcgaacgcct cgccgcgttc
     1441 ggcggcaaag gccagcatcg gcagatcgtt gatcgagtcg ctgtaggcct ccaacctgag
     1501 gccgtcatca gcagcgccca gactctccag aaactccctg aggagcacca gcttgccctc
     1561 gccgtaagag tgggaccctg caattcggcc cgtgaactct ccgaaggcgt cctcttcagg
     1621 tcgagccgcc acaagatgcc tgatgccgaa gagcggcgca atcgcacccg tcacgaaggc
     1681 atgcgtgcct gaagcaagca cgggctcaaa ccccgccttc ctgcgggatt cgaccagatc
     1741 gagcgcttcg gggcggaggt gcggcctgac gcaggcatcg atgtaggctg tgcaccactt
     1801 ctcaagatcc tgccgcctgc aggcggccag aagccccgcc tgaaagccca tgaactcgtc
     1861 ggcgctgaat gcacccttgc ggtaggcttc ggcataggcc tcgatccggg cgtccatcag
     1921 ggcgacatcc atgcctgcgg ctctcacgag ccagcggctc cagttctctc cgctgtcgac
     1981 ggggagaagc gtgtgatcaa gatcgaagac tgcaaacttc atgtcgtgaa aaaacgggat
     2041 cagtatgcct gacgggcgtg ctgcctctca tcaagagagg tcacggcctc gcgcaacaga
     2101 ggaagcgtca cgcgacgctg cttctgcaac ccgaggcggt ccgcttcatc aagcacacag
     2161 gtgagcgtgc gcatgtcgcg cggcaggttg gcccccatcc agtgaaggat gtcaggcgtg
     2221 agctcgatgc cgcgtcgtgc ggcctgacgg tgaagctcgg caagacgctc ctcatcgtca
     2281 agcggacgaa ttgcatagca gagtccccag gccagacgac ttctgacaac ttcgggaagc
     2341 ggaagctgag cggcgggcac cctgcccgca cacacgagat gcatgcccgg cgttccgcgc
     2401 acggcattct gcagtgcaag cagctgcaac gcccagcccg gatctagttc gtccacgtcg
     2461 tcaacgacgt agagcggcac cttcggatcg aacatcggca cgcggaagcc ctcttcaggc
     2521 accagactcc tcagcaaatg cgtgaggccc gcccccttgg gaccgtaaag atacacgaag
     2581 gtgggcccgc agcctgcggc cacttctcgc agaacactga cggcttcgcc gttggcaccg
     2641 acaacgaagt tgtcgagcgt cggcttgtcg cgctcctgca ggtcgagcag ata
//
LOCUS       DBMDTI010000279         2663 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8548_length_2663_cov_2.133099, whole genome shotgun sequence.
ACCESSION   DBMDTI010000279 DBMDTI010000000
VERSION     DBMDTI010000279.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8548_length_2663_cov_2.133099"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            390..1505
                     /locus_tag="ACZPB6_09600"
     CDS             390..1505
                     /locus_tag="ACZPB6_09600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR1184754.1"
                     /translation="MSLNAVNHRRVLHRMPELDDELPDTTAYVRKVLEPLGAEVFSPI
                     KGSVCAYFDAGAEETVALRADMDALPVTECTGLFFASERPGRMHACGHDGHTAIELGL
                     AEETARMIRAGEKLPRNVFFVFQPAEETTGGAGRICETGVFEKYRVTRIFGLHLWPNL
                     PEGSVWSRPGPMMARSNEVTLKVTGRSVHVSRAAEGLDALRAGVDWMNAAYAWTEALP
                     PDVLRTLQFGRMTAGTVRNAVAGSAEIQGTLRTYDEDAAEMIRSHLRTLAAETAERTG
                     CGLEVSFSTGYPAVWNNETLFERVSRDLGADAPQLLAEPVLAAEDFSFYQRCIPGVFF
                     FLGVGPAHELHSPHFCWNDEAVIPAGIAFMKKIMVLP"
     gene            1681..>2663
                     /locus_tag="ACZPB6_09605"
     CDS             1681..>2663
                     /locus_tag="ACZPB6_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1184755.1"
                     /translation="MDFLNQLVSDVNGVLWGVFFLIPLLCGTGIFYTIKLRFVQVRKF
                     GLAARFLLGGASLFGKRADKSGMSSFQALATAVAAQVGTGNVAGTATALVAGGPGSLF
                     WLWVAAFFGMATIFAEAVLAQTYRTKDEQGRVLGGPAYYITLGMGEKWRPLAVFFSIA
                     IIIALGCIGNMVQSNSIAHAMQTAFNVPAAVSGVVCAVLAGLVFFGGVGRLASVTEKI
                     VPFMAAIYLIGGLWVIGAHITEVIPAFRLIFVAAFDPEAVMGGALGLTVAKAMQFGVA
                     RGLFSNEAGMGSTPHAHAVARVEHPAEQGLVAMFGVFTTVVIVTFTGIVILV"
BASE COUNT          421 a          896 c          840 g          506 t
ORIGIN      
        1 ggacggacag tccgcaggat gttctcctgc gacccatcac cccggagcgc cctccgcgga
       61 tgattcggcg gtgttgcctc gccacgacgt cgtcgcctgc cggctcggtc gggcccttcg
      121 tccccgctcc tctatctgca ggagcaaagt taccacggac ggcccgattt tgaaaaggga
      181 cggcgtcgga aaagacgctc gggtgggtcc cgcgcaaacc gtctaggtga aacgccctac
      241 gacctattgc ctaggtgcgc agtttcgact cttccgaagc tccgccacat gatataaatg
      301 caggactttt catcgagcgc cggcttcggg ggcgagagcc tcctggcggc ctttccgtcc
      361 ttgtgtctgc acttccggag aacgcgtcca tgagcctcaa tgccgtcaac caccgcaggg
      421 tcctgcaccg aatgcccgag ctcgacgacg agctgcccga tacgacggcc tacgtgagga
      481 aggtgctcga gccgctcggc gccgaggtct tctcgcccat caagggcagc gtgtgcgcgt
      541 acttcgacgc gggcgcagag gagacggtcg ccctgcgcgc cgacatggat gcgctgcccg
      601 tgaccgagtg cacggggctt ttcttcgcct ccgagaggcc ggggcgcatg cacgcctgcg
      661 ggcacgacgg ccacacggcc atcgaactcg gtcttgccga ggagacggcc cgcatgatca
      721 gggcgggcga gaagctgccg aggaacgttt tcttcgtctt ccagcccgcc gaggagacga
      781 cgggcggcgc gggacgcatc tgcgagacgg gcgtcttcga gaagtaccgc gtcacgcgaa
      841 tcttcggcct gcacctctgg cccaaccttc ctgagggaag cgtctggtcg cgtcccggcc
      901 cgatgatggc ccgctcgaac gaggtgacgc tcaaggtgac gggcaggagc gtgcacgtct
      961 cgcgcgccgc cgaggggctc gacgccctgc gagcgggcgt cgactggatg aacgccgctt
     1021 acgcctggac cgaggccctg ccgccggacg tcctgcgcac gctgcagttc ggccgcatga
     1081 cggcgggcac ggtgcgaaac gccgtcgcgg gctccgccga aatccagggc acgcttcgca
     1141 cctacgacga ggatgccgcg gagatgatcc gctctcacct caggacgctc gccgccgaaa
     1201 cggcggagcg aactggctgt gggctcgaag tgtccttcag cacgggctac cccgccgtct
     1261 ggaacaacga gacgctcttc gagcgggtct cgagggacct cggcgcagac gccccgcagc
     1321 tgctcgctga acccgtcctc gcggcggagg acttctcctt ctaccagagg tgcattcctg
     1381 gcgtcttctt cttcctcggc gtggggcccg cgcacgaact ccactccccg cacttctgct
     1441 ggaacgacga ggccgtgatc cctgcgggca tcgccttcat gaagaagatc atggttctcc
     1501 cttagaataa tggttcttcc ctaagtggaa attcttattg cattttgcat tagaatgcta
     1561 gttccactct gccgctgaca gtgaaagaag gttgggacgg ccggaacgga aaacagaacc
     1621 gaataacgag aggggccctg cgagggccct tcgtgtccat cttttccatc caagctacaa
     1681 atggatttcc tcaatcaact cgtcagcgac gtcaacggcg ttctctgggg cgtcttcttc
     1741 ctgattccgc tgctttgcgg cacgggcatc ttctacacca tcaagctgcg cttcgttcag
     1801 gtgcgcaagt tcggtcttgc ggcccgcttc cttctgggcg gagcgtcgct cttcggcaag
     1861 cgtgccgaca agtcgggcat gagctccttt caggcgcttg ccacggccgt tgcggcccag
     1921 gtgggcacgg gcaacgttgc gggcacggcc acggccctcg tggcgggcgg gccgggctcg
     1981 ctcttctggc tctgggtcgc ggccttcttc ggcatggcaa ccatcttcgc cgaagccgtg
     2041 ctcgcgcaga cctatcgcac gaaggacgag cagggccgcg tgctcggcgg tcccgcctac
     2101 tacatcacgc tcggcatggg cgagaagtgg cgtccgcttg cggtcttctt ctcgatcgca
     2161 atcatcattg cgctgggctg catcggcaac atggtgcagt ccaactccat cgcgcacgcc
     2221 atgcagaccg ccttcaacgt gccggccgcc gtctcgggcg tcgtgtgcgc ggtgctcgcg
     2281 ggcctcgtct tcttcggcgg cgtcggccgt ctggcgagcg tcaccgagaa gatcgttccc
     2341 ttcatggcgg ccatctacct catcggcggc ctctgggtga tcggcgccca catcaccgaa
     2401 gtgattcccg ccttcaggct catcttcgtc gcggcctttg atcccgaggc cgtcatgggc
     2461 ggcgcgctcg gcctgacggt cgccaaggcg atgcagttcg gcgtggcccg cggcctcttc
     2521 tcgaacgagg ccggcatggg ctcgaccccg cacgcgcatg ccgtcgcccg cgtcgagcat
     2581 cccgccgaac agggcctcgt cgcgatgttc ggcgtcttta cgacggtcgt gatcgtgacc
     2641 ttcacgggca tcgtcatcct tgt
//
LOCUS       DBMDTI010000280         2655 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8574_length_2655_cov_2.056382, whole genome shotgun sequence.
ACCESSION   DBMDTI010000280 DBMDTI010000000
VERSION     DBMDTI010000280.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8574_length_2655_cov_2.056382"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="ACZPB6_09610"
     CDS             <1..441
                     /locus_tag="ACZPB6_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA family protein"
                     /protein_id="HIR1184756.1"
                     /translation="AGRTLTSTGTLSLTGDGAFSWVQKTPFVQTVSLKDGVFTIRYED
                     EAPEVMTAETHPAVLSAARLLTSVMRLDAAALQDAFDYTLVGGEKPADAWTITLRARD
                     EALGRILGTVTLKGSEYVESIRMSPSNDNVTDMILSNVRIVKGA"
     gene            638..>2655
                     /locus_tag="ACZPB6_09615"
     CDS             638..>2655
                     /locus_tag="ACZPB6_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR1184757.1"
                     /translation="MTDQFSIITTLVTGFGLALVFGWLAERFLKTPTLVGYIIAGVSV
                     GLFPLLPNVDRVVTEQFAEIGVMLLMFGVGLHFSVADLVKVKGVAVTGAMLQMAASGA
                     AGTVVALLAWDWALPNAIVFGMTLSCASTVVVMKALELRKLSVTPSGQVAIGWLVVQD
                     LVTVFIMVCLPLVAQVTQGEGSVDPRLIVISLSKTLLGVAVFVAGMLIVGKRLFPWIL
                     QRVALSGSRELFTLCVLAIAIGIAYGAGAIFNVSYALGAFFAGMVMRESSFAHRAARN
                     CLPLQDAFSVLFFISVGLMLDPYVFVNEPWAVIAVMLIIMCITSSVTAALVLLLGWPL
                     DTALVMGACVSQLGEFSFILCGQGIALGIATPGTMSIVVAASILTIALNPLMFTAIPH
                     VRNLLVSRRPFFARMAERVSPLASVEPKTIKGHVLIAGVNEVVLALLPRLEARGIDFT
                     CFVMSDTTAAVVDSYKHGSAIVGNPSDPALLVQGHLVDAEVLVLPSSDMVLNKTVFAA
                     ARELKHDCRIIVRVASPENLRAVPSDEHTRILNDTQIASESLGEAVADVFGIGAQVRH
                     EKAVARGEATNDIDEDEGAAIIAQARAEAAASAARHAARNTSRRSIFDGIREKMAKRR
                     EEKARAKAAVKAAQAAQAAEGGEAADAAFDASAVPEAQKPSFFSGMKD"
BASE COUNT          428 a          898 c          816 g          513 t
ORIGIN      
        1 gcgggccgca cgctcacgag cacgggtacg ctgtcgctca cgggtgacgg agccttctcc
       61 tgggtccaga agacgccttt tgtacagacg gtctcgctca aagacggcgt cttcacgatc
      121 cgctacgagg acgaggcgcc cgaagtgatg acggccgaaa cccatcccgc tgttctttcc
      181 gccgcacgtc ttctcacgtc cgtgatgagg ctcgacgcgg ccgcgctgca ggacgccttc
      241 gactacacgc tcgtgggtgg cgaaaagccc gccgacgcct ggacgatcac gctcagggcc
      301 cgcgacgagg ctctcggccg cattctcggc accgtgacgc tgaagggctc ggaatacgtt
      361 gagtcgatca gaatgtctcc ttctaacgac aacgtgaccg acatgattct ctccaacgtg
      421 aggatcgtga aaggggcctg aattcgatat cgtcgaagcg acgaggcttt tgaagtttta
      481 agctttcgaa cccgctgagt cgcttcggga cgacgctgag gcggagtgcc cgttggaaga
      541 gtagacttaa acactttctc tccgtgcaaa gtgcccgccc cgcgcagcgg aacggagagg
      601 aacggaccag actggacaga ccagaggaga agcggccttg accgatcagt tttccatcat
      661 caccacgctc gtgacgggct tcgggcttgc gcttgttttc gggtggcttg cagagcgctt
      721 cctgaagacc cccacgcttg tgggctacat catcgcgggc gtgtccgtgg gcctcttccc
      781 gctccttccc aacgtcgacc gcgtcgtcac ggagcagttc gccgaaatcg gcgtgatgct
      841 cctgatgttc ggcgtgggcc tgcacttctc ggtcgccgac ctcgtgaagg tcaagggcgt
      901 ggccgtcacg ggcgcgatgc tgcagatggc cgcatcgggc gccgccggca cggtcgtcgc
      961 gctccttgcc tgggactggg cgcttcccaa tgcgatcgtc ttcggcatga cgctctcctg
     1021 tgcatcgacc gtcgtcgtga tgaaggccct tgagcttcgc aagctttccg tcacgccgag
     1081 cggtcaggtc gccatcggct ggctcgtcgt gcaggacctc gtcaccgtct tcatcatggt
     1141 gtgcctgccg ctcgtcgcgc aggtgacgca gggcgagggg tccgttgatc cgaggctcat
     1201 cgtcatctcg ctctccaaga cgctgctcgg cgtcgcggta ttcgtggccg gcatgctcat
     1261 cgtcggcaag cgtctcttcc cgtggattct ccagcgcgtg gcgctctcgg gttcccgcga
     1321 gctcttcacg ctctgcgtgc ttgcgatcgc catcgggatc gcctacggcg cgggtgcgat
     1381 cttcaacgtg agctacgctc tgggcgcctt cttcgcaggc atggtgatgc gcgagtctag
     1441 ctttgcgcac cgtgcggccc gcaactgcct gccgttgcag gacgcgtttt cggtgctgtt
     1501 cttcatctcc gtgggcctca tgctcgaccc gtacgtcttc gtgaacgaac cctgggccgt
     1561 gatcgccgtg atgctcatca tcatgtgcat cacgagcagc gtgacggccg cgctcgtgct
     1621 gcttctgggc tggccgctcg acacggccct cgtcatgggc gcctgcgtct cgcagctcgg
     1681 tgaattctcc ttcattctct gcggccaggg cattgcgctc gggatcgcga cgccgggcac
     1741 gatgagcatc gtcgtggcag cctcgattct gacgatcgcg ctcaatccgc tgatgttcac
     1801 tgccatcccg cacgtgagga acctcctcgt ctcccgccgt ccgttctttg cgcgcatggc
     1861 cgaacgcgtc tcgccgctcg cctccgtcga gcccaagacc atcaaggggc acgtgctcat
     1921 cgcgggcgtc aacgaggtcg tactcgcgct cctgccgagg ctcgaggctc gcggcatcga
     1981 cttcacctgc ttcgtgatga gcgacacgac cgccgcggtc gtcgactcct acaagcatgg
     2041 ttcggccatc gtcggcaatc cgtccgaccc cgcgctcctc gtccagggcc atctcgtcga
     2101 tgccgaggtc ctcgtgctgc cgtcctccga catggtcctc aacaagaccg tctttgcggc
     2161 cgcgcgcgaa ctcaagcatg actgccgcat catcgtgcgc gtcgccagcc ccgaaaacct
     2221 cagggctgtg ccgtccgacg aacacacccg catcctcaac gacacccaga tcgcctccga
     2281 aagcctcggt gaggccgtgg ccgacgtctt cggcatcggc gctcaggttc gccacgaaaa
     2341 ggcggtcgca cgaggcgagg cgacgaacga catcgacgag gacgaaggcg ccgccatcat
     2401 cgcgcaggcc cgcgccgaag ccgccgcgag cgccgcacgg catgcggccc gaaacacctc
     2461 ccgccgttcg atcttcgacg gcatcaggga gaagatggcg aagcgccgcg aggaaaaggc
     2521 gagggcgaag gccgccgtca aggccgccca ggccgctcag gctgccgagg gtggtgaagc
     2581 cgcagacgcc gcgttcgatg cgtccgccgt tcccgaggct caaaagccct ccttcttctc
     2641 cggcatgaag gacaa
//
LOCUS       DBMDTI010000281         2651 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8591_length_2651_cov_2.514510, whole genome shotgun sequence.
ACCESSION   DBMDTI010000281 DBMDTI010000000
VERSION     DBMDTI010000281.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8591_length_2651_cov_2.514510"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(97..1029)
                     /locus_tag="ACZPB6_09620"
     CDS             complement(97..1029)
                     /locus_tag="ACZPB6_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1184758.1"
                     /translation="MMKQSLWALAAAALFSVMAAFVKLCSDVHGPIEMVFYRSLFGVL
                     VISLFVFRNRATLTLKTPHIAGHIKRSVLGTLSIMLWFFTLGKMHFGTNMTLIYTTPL
                     FMAANFVILALMRHKPAPWALVLATIAGFTGVTIVLQPSFNEGELIPALICLGVSLLD
                     LVIYWQMKELGDMKEPSWRIVFYFTLFGTIAGLIACYVVEGGLHMPNAQGLVGILGMG
                     LCATLGQICTTRSYAYGNMLLSSCLGFSAIPFSVIISFFWFGESVTAATLTGAAVILC
                     SGMMATVATKRTEAAEKAARAAEEAAKAETEKTA"
     gene            complement(1253..2125)
                     /gene="serB"
                     /locus_tag="ACZPB6_09625"
     CDS             complement(1253..2125)
                     /gene="serB"
                     /locus_tag="ACZPB6_09625"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HIR1184759.1"
                     /translation="MLDLILAGPGVTPDLVCDLADEFMAGEVLTRPNAARFCGLSPMT
                     ARSIARRLADDESIDVLEVKAGLSVWDFRVICLDMDGTVIQNECIDDMAALHGVGEEV
                     SSVTRAAMEGGTKLTFAESLEKRVAFLRDAPVSVIDEALASVRLQPGAARLIDFCNAH
                     GIRSYVLSGGFTHFTHAIATRLGMTGDFSNMLGIDAERGVLTGTVTGPAGGRILDADG
                     KRRTLEILCHQAGATLEQAIAAGDGANDLEMIGAAALGIAYHAKPVVRAQARLAVTAS
                     GLDAITLLFIEAWR"
     gene            complement(2454..>2651)
                     /locus_tag="ACZPB6_09630"
     CDS             complement(2454..>2651)
                     /locus_tag="ACZPB6_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine ammonia-lyase"
                     /protein_id="HIR1184760.1"
                     /translation="YSVEMLHAAQAVDLRRNLKNKDLKLADKTGAFYTAYRAKVPYVK
                     ADRIFTDDIRAGVDLLKNYRF"
BASE COUNT          505 a          714 c          995 g          437 t
ORIGIN      
        1 aattcggagg cttcctgcga ctgtttctgt gaaaacgggc ctgcatccgt ggcaggatgc
       61 aggcccgttc tgtcggtgac gcgtcgcgga cggaggtcag gcggtctttt cggtttcagc
      121 cttggccgct tcctcggcgg ccctagcggc cttttcggcg gcctcggtgc gcttggtggc
      181 gaccgtggcc atcatgcccg agcagaggat gacggcggcg cccgtgaggg tcgcggcggt
      241 cacgctctcg ccgaaccaga agaagctgat gatgaccgag aacggaatcg cggagaagcc
      301 caggcacgag gagaggagca tgttgccgta ggcgtaggag cgggtcgtgc agatctggcc
      361 gagcgtggcg cacaggccca tgccgagaat gccgacgagg ccttgggcgt tgggcatgtg
      421 caggccgccc tcgacgacgt agcaggcgat gaggcccgcg atggtgccga agagcgtgaa
      481 gtagaagacg attcgccagg agggctcctt catgtcgccg agctccttca tctgccagta
      541 gatgacgagg tcgaggagcg agacgcccag acagatgagg gccgggatca gctcgccctc
      601 gttgaagctc ggctgcagca cgatggtgac gcccgtgaag ccggcgatcg tggcgagcac
      661 gagcgcccac ggcgcgggct tgtggcgcat caaggcgagg atcacgaagt tggcggccat
      721 gaagagcggc gtcgtgtaga tgagggtcat gttggtcccg aagtgcatct ttccgagcgt
      781 gaagaaccag agcatgatcg agagcgtgcc gaggacggaa cgcttgatgt ggcccgcgat
      841 gtgcggggtc ttgagggtga gggtagcgcg gttgcggaag acgaagaggg agatcacgag
      901 cacgccgaag agcgagcggt agaagaccat ttcgatgggg ccgtgtacgt cgctgcagag
      961 cttgacgaat gccgccatga cggaaaagag cgccgcagcc gccaacgccc aaagggattg
     1021 cttcatcatt ttattttctt cttgaagacg gtttgtgggg gtggttttgg ggtgacacga
     1081 ttttagccaa acctgcggtc caaaagaaga cgcaaaaggc ctgcggcagg ggattcgagg
     1141 gaaaacgtga acactaaaga caacacccgc agagcggtgc gctgcgggtg tcggggaaca
     1201 ggcgaaaacg ggctgccggc gtcctgcgga tgagcggcgg acgggcggct cgtcagcgcc
     1261 aggcttcgat gaagaggagc gtgatggcgt cgaggcccga ggccgtcacg gcgaggcggg
     1321 cttgggcgcg cacgacgggc ttggcgtggt aggcgatgcc gagggcggcc gcgccgatca
     1381 tctcgagatc gttggcgccg tcgcctgcgg cgatggcctg ttcgagcgtg gcgcccgcct
     1441 gatggcagag gatctcgagc gtgcggcgct tgccgtcggc gtcgaggatg cggccgcccg
     1501 cggggcccgt cacggtgccc gtgaggacgc cgcgttcggc gtcgatgccg agcatgttgc
     1561 tgaagtcgcc cgtcatgccg aggcgggttg cgatcgcgtg cgtgaagtgc gtgaagccgc
     1621 ccgagaggac gtagctgcgg atgccgtggg cgttgcagaa gtcgatgagg cgtgcggcgc
     1681 cgggctgcag gcgcacgctc gcgagggcct cgtcgatgac cgagacgggg gcgtccctga
     1741 ggaaggcgac gcgcttttcg aggctttcgg cgaaggtgag cttcgtgccg ccctccatgg
     1801 cggcgcgcgt gactgaggag acctcctcgc ccacgccgtg aagcgcggcc atgtcgtcga
     1861 tgcactcgtt ctgaatgacg gtgccgtcca tgtcgaggca gatgacgcgg aagtcccaga
     1921 cggaaagccc cgccttcact tcgaggacgt cgatcgattc gtcgtccgcc aggcgccgtg
     1981 cgatcgagcg ggccgtcatc ggggagaggc cgcagaagcg tgcggcattg gggcgcgtga
     2041 ggacttcgcc cgccatgaat tcgtcggcga ggtcgcagac gagatcgggg gtgacgccgg
     2101 ggccggcaag aatcagatca agcatggaaa gccttggaga aatgagtgga cgcagtcctt
     2161 caaatgtagc ccaaggcgga tgaagacgca aacgaggaag gcgtcctgga aggggtcggg
     2221 aacgtgaaaa aaaaggagcg gcgtcccgat acgcacacct cgggaaggcc gctccaaaaa
     2281 gaggggaaga tcctcttcat gaagaaggac gcaggagaac cgtccttcgg aaagaaaaga
     2341 gagcaaccgg cgggatcccc cgggaagccg tctcgtcagg aggcattgcg agacggctcc
     2401 ccgaacctga ctgcggcggg ggattctgtc ggatatgcgg cgaagcgtcc gcctcagaac
     2461 ctgtagttct tgaggaggtc gacgccggcg cggatgtcgt cggtgaagat tcggtcggcc
     2521 ttgacgtacg gcaccttggc gcggtaggcg gtgtagaagg cgccggtctt gtcggcgagc
     2581 ttcaggtcct tgttcttcag gttgcgacgc aggtcgacgg cttgggcggc gtggagcatt
     2641 tcaaccgagt a
//
LOCUS       DBMDTI010000282         2643 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8634_length_2643_cov_1.738002, whole genome shotgun sequence.
ACCESSION   DBMDTI010000282 DBMDTI010000000
VERSION     DBMDTI010000282.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8634_length_2643_cov_1.738002"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            170..>2643
                     /locus_tag="ACZPB6_09635"
     CDS             170..>2643
                     /locus_tag="ACZPB6_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184761.1"
                     /translation="MMILARTELATIDALRASGEEPERILELIESIVNKGIDTGRFDM
                     CHVETDPEAALRLAEALLGTNRYPAFARAMRILETVTPYVEDRSDGMLLFTFSTIARL
                     LCGRPFDAAGRAVSGIGEDERDVRLLYLLGILRAGDGRFDEAREWLLRADVESPDDPV
                     ILHALELVAARESLERILLTRPLWEDGDLDHTAPGTPERALRMACVELDPDRYADALI
                     ALNATGLELDDESFLRMKVELSGHAVPFVFEMNHAGLSKMDPAWLARLVESVSDVLEH
                     AKAAPEHLVEVRARLDRSVRLVLTKPEPDTTRPSAIDYSFEVPLRCPRLEGRLDTGPE
                     EAAVLKHLRALDLRGNFDEIIRRLEAVQKEKFTPLYAFELARAIANRSESGKDDLKRA
                     LALLEGLDDRTEGRWETLFCRAFLNLRLGQRRAALKCFKACAGMRPDDEVIDFFVEVC
                     LRGLSQPTIEKPFRVRADEVWAALEAQATELEEAFDSITDADEVEKRLAGILEPVSAS
                     WWRLDVSAEDERIKVEISPNGWRLECFPILEFLRRVPESLSDRWIFTAGRARRDDTSF
                     ETFMAGNEVLSADDVLIWPEPHEGMWRLLVYIEDFKGTEERARIECFQAVRALIDRTV
                     GEAARLRRADAITLVPKAPSGEGLTLSEFREFLFEKAPECRRMTLKDYAWLPHEYGMR
                     PDTRPGAPLLADVTEGITHCPPLGYLYGQGKSIGMDALGAAGACAGFLFLDVKDDAEG
                     LAPKKDRRIKARKMLEVRLRGLGDGYLDVVGHASGVRYEYLQFIAWDLSAVLECASAA
                     LKDFDDVVEAGFHTYLRDASAFMIGV"
BASE COUNT          473 a          888 c          823 g          459 t
ORIGIN      
        1 atccagccgt ccgagtcgac cgattcgggc ggcttttcag ttcatgcatg ccgctcatga
       61 agccatgacc gccattcctt cctgaaggag aaggacgcgg gggattcgta gttttcaaac
      121 cttctgtaag atatccgaac acggtttcaa aacaagaaca ggagtcagga tgatgatcct
      181 cgcaagaacg gagctcgcga ccattgacgc actcagggcc tcgggtgagg agcccgagcg
      241 gatcctcgag ctcatcgaga gcatcgtgaa caagggcatt gataccggtc gcttcgacat
      301 gtgccacgtc gagaccgatc cggaagccgc gctcagactc gcagaggcgc tgctcggtac
      361 caacaggtat cccgccttcg cgcgcgcgat gagaattctt gagacggtga cgccgtacgt
      421 ggaggaccgg tccgacggca tgcttctgtt cacgttctcg acgatcgcgc gcttgctctg
      481 cggccgaccc tttgatgcag cgggccgcgc cgtctccggg atcggagaag acgaaaggga
      541 cgtccgcctt ctttatctgc tcggcattct tcgcgcaggc gacggacgct ttgacgaggc
      601 ccgcgaatgg cttctgcgcg ccgatgtcga gagtcccgat gatcccgtca tccttcacgc
      661 cctcgagctc gtggcggcac gcgagtcgct tgagcgcatc cttctcaccc gcccgctctg
      721 ggaggacgga gaccttgacc acacggcgcc gggaactccg gagcgcgccc ttcgcatggc
      781 ctgcgtcgag ctcgatcccg acaggtacgc agacgccctg atcgcgctca acgcgacggg
      841 gctcgaactc gacgacgaga gcttcctgcg catgaaggtc gagctctccg gccacgccgt
      901 gccgttcgtt ttcgagatga accacgcggg cctctcgaag atggatcccg cctggctcgc
      961 ccgtcttgtc gaatcggtct ccgacgtgct cgagcatgcg aaggccgcgc ccgagcacct
     1021 cgtagaagtc cgcgcaagac tcgacaggag cgtgcgcctc gtgctcacca agcccgagcc
     1081 cgacacgacg cggccctccg cgatcgacta ttcctttgaa gtccccttgc gctgtccgcg
     1141 gctcgagggt cgtctcgaca caggacccga ggaggccgcc gtcctcaagc acctgcgcgc
     1201 ccttgacctg cgcggcaact tcgacgaaat catcaggcgc ctcgaggccg tccagaagga
     1261 gaagttcacg ccgctctatg ccttcgaact cgcccgcgcg atcgccaacc gaagcgaatc
     1321 cgggaaggac gacctgaaaa gggcgcttgc gctgctcgag ggtctggacg accggaccga
     1381 aggccgctgg gagacgctct tctgccgagc cttcctcaac ctgaggctcg gccagaggcg
     1441 cgcggccctc aagtgcttca aggcctgcgc gggcatgagg cccgacgacg aggtgatcga
     1501 cttcttcgtc gaggtctgcc tgcgcggcct ctcgcagccc acgattgaaa agcccttcag
     1561 ggttcgcgcg gacgaggtct gggcggcgct tgaagcgcag gcgaccgagc tcgaagaggc
     1621 gtttgattcc atcacggacg ccgacgaggt cgaaaaacgc cttgcgggca tcctcgagcc
     1681 cgtctccgcc tcctggtggc gccttgacgt gagcgcggag gacgagcgca tcaaggtcga
     1741 aatctcgccc aacggatggc gcctcgaatg cttcccgatc cttgaattcc tcaggcgcgt
     1801 ccccgaatcc ctttccgacc gctggatctt cacggcgggc agggccagaa gggacgacac
     1861 ctccttcgag accttcatgg cgggcaacga ggtgctctct gcggacgacg tgctcatctg
     1921 gcccgagccc cacgagggca tgtggcgcct tctggtctac atcgaggact tcaagggcac
     1981 cgaagagcgc gctcgaatcg agtgcttcca ggccgttcgc gccctcatcg accgcacggt
     2041 cggcgaggcc gcccgcctgc gccgcgccga cgccatcacg ctcgtgccga aggccccttc
     2101 gggcgaaggc ctcacgctct ccgaattcag ggaattcctc ttcgagaagg cgcccgaatg
     2161 caggcgcatg acgctcaagg actacgcctg gctgccgcac gagtacggca tgcggcccga
     2221 cacgaggccc ggtgcgccgc ttctggccga cgtcaccgag ggcatcacgc actgcccgcc
     2281 gctcggctac ctctacggcc agggcaagag catcggcatg gacgctctcg gcgccgcagg
     2341 cgcctgcgcg ggcttcctct tccttgacgt gaaggatgat gccgaaggac tcgcgccgaa
     2401 gaaggaccgt cgcatcaagg cccgcaaaat gctcgaggtc cgactgcgcg gactcgggga
     2461 cggatatctg gacgtcgtgg gccacgcctc gggcgtgcgc tacgagtacc tgcagttcat
     2521 cgcctgggac ctctcggccg tgctcgagtg cgcttcggcc gcccttaagg acttcgacga
     2581 cgtcgtcgag gcgggcttcc acacctatct gcgagatgcc tcggccttca tgataggtgt
     2641 gga
//
LOCUS       DBMDTI010000283         2641 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8640_length_2641_cov_1.845669, whole genome shotgun sequence.
ACCESSION   DBMDTI010000283 DBMDTI010000000
VERSION     DBMDTI010000283.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8640_length_2641_cov_1.845669"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            121..393
                     /locus_tag="ACZPB6_09640"
     CDS             121..393
                     /locus_tag="ACZPB6_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HIR1184762.1"
                     /translation="MAECAHEWTPGEEAAHDEEKLVVKDANGTLLADGDSVTIVKTLK
                     VKGASTPLKIGTKVKNSRLVEGDHNIDCRIDGFGAMQLKSEFVKKL"
     gene            complement(510..2537)
                     /locus_tag="ACZPB6_09645"
     CDS             complement(510..2537)
                     /locus_tag="ACZPB6_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HIR1184763.1"
                     /translation="MKLFIAEKPSVAKAIAAELGVSGRGDGFINCRNDVVVTWCFGHL
                     LEQAEPDAYLPDDIPKTKKGRKIWRMQDLPIFPQHWLMEAKSERGAKKQLSVIGKLLK
                     SAATVVNAGDPDREGQLLVDEVLEHFRARQPVERFWVSAVDPASIRKGLAKLGDNCSY
                     AGMRDAARGRSRADWLLGMNLSRAYTLAGGDGLLAVGRVQTPTLFMVARRDYVVRNFT
                     PVPYLSITADLAKKTVPFTARWKPGPDQKGLDEEGRLLIDLDVGRALVERLSKEKTAT
                     VLSAETKRRKANPPKAYSLADIQIEASKRFGMTAENTLKVCQSLYEVRKITSYPRTDC
                     GYLPESQHADAPAVLAAVARNFPAAAPACAKADPKLKSPTFNDKKVTAHHGIVPVANT
                     IDPATLTREEADIYRLIVRRYIAQFFPVHEFDATEVVLGIGGETFTAKGRVVRVEGWR
                     ILFEKDRRAAEEKRRKNPKAAGGRDPDGEDEDDAQTLPALKKGDVCDVRAVKGREDKT
                     KPPQFFTEGTLIAAMENIWRSFDDPKGQAMLKEAGGIGTPATRAAIIAELRRKEYLET
                     EGKYLHCTESGRRLLKRVSPRIRSAAMTAHFEERLRLVESGAEQLDNFVSEYEAFIAA
                     ELQKVRAGRTAPSGSARTGAARPAPAPSREPSPEAPAPNMPDEPPPLDTVC"
BASE COUNT          388 a          802 c          929 g          522 t
ORIGIN      
        1 aaaggcagga aaggtattgc tatttgatta tatgaggctt cctattttgg cgtatgcctc
       61 gcattttgct gtcttttccg ccattcctcc ccgcctcaca ggcgagggtt cctggcggat
      121 ttggctgaat gcgcccatga atggacgccc ggcgaggaag ccgcccatga cgaggagaag
      181 ctcgtcgtga aggacgccaa cggcacgctg ctcgccgacg gcgactccgt caccatcgtg
      241 aagaccctca aagtgaaggg tgcctcgacg ccgctcaaga tcggcaccaa ggtgaagaac
      301 agccgcctcg tcgaaggcga ccacaacatc gactgcagga tcgacggctt cggcgcgatg
      361 cagctcaagt ccgagttcgt taagaagctc tgacgcagga cgtcgtcctg cgaggatcca
      421 tccggacgcg aaaggttccg cttctctccg tttcgacgga ggaaggcgga gccttcttcg
      481 tttgagggcc gcgcgggtct tccggacgat cagcagaccg tatcgagcgg tggcggctcg
      541 tcgggcatgt tcggggcggg cgcttcgggc gacggctcgc gggacggtgc gggtgcgggc
      601 cttgccgctc cggtgcgtgc ggagccgctc ggggccgtgc ggcctgcgcg gaccttctgc
      661 agttcggccg cgatgaaggc ctcgtattcg ctcacgaagt tgtcgagctg ctcggcgccc
      721 gactcgacga gcctgaggcg ctcctcgaag tgtgcggtca tggcggccga gcggatgcgg
      781 ggcgaaaccc gcttgaggag cctgcggccc gattcggtgc agtgcaggta cttgccttcg
      841 gtctcgaggt attccttgcg cctgagttcg gcgatgatgg ccgcgcgggt ggccggcgtg
      901 ccgatgccgc ccgcctcctt gagcatggcc tgtcccttcg ggtcgtcgaa gctgcgccag
      961 atgttctcca tggcggcgat gagcgtgccc tcggtgaaga actgcggcgg cttcgtcttg
     1021 tcctcgcgtc ccttgacggc gcgcacgtcg cagacgtcgc cctttttgag tgcggggagc
     1081 gtctgggcgt cgtcctcgtc ctcgccgtcc ggatctcggc cgcctgcggc cttcgggttc
     1141 ttcctgcgct tttcctcggc agccctgcgg tccttctcga agaggatgcg ccagccttcg
     1201 acgcgcacga cgcggccctt ggcggtgaag gtctcgccgc cgatgccgag cacgacctcg
     1261 gttgcgtcga actcgtgcac cgggaagaac tgcgcgatgt agcgcctcac gatgaggcgg
     1321 tagatgtcgg cttcctcgcg ggtgagcgtg gcgggatcga tcgtgttggc gacgggcacg
     1381 atgccgtggt gcgccgtcac cttcttgtcg ttgaaggtcg ggctcttgag cttcgggtcg
     1441 gccttggcgc aggcgggggc tgcggcgggg aagttcctgg cgacggcggc gagcacggcg
     1501 ggcgcgtccg cgtgctggga ttcgggcaga tagccgcagt cggtacgagg atagctcgtg
     1561 atcttcctga cttcgtagag cgactggcag accttgagcg tgttctcggc cgtcatgccg
     1621 aagcgcttgc tcgcctcgat ctggatgtcg gcgagcgagt aggccttggg cggattggcc
     1681 ttgcggcgct tcgtctcggc ggagagcacc gtggcggtct tttccttcga gagacgctcg
     1741 acgagcgcgc ggccgacgtc gaggtcgatg aggagcctgc cttcctcgtc gaggcccttc
     1801 tggtcgggac cgggcttcca gcgtgcggtg aaggggacgg tcttcttggc gaggtccgcc
     1861 gtgatcgaga ggtagggcac gggcgtgaag ttgcgcacca cgtagtcgcg tcgtgcgacc
     1921 atgaagagcg tcggcgtctg cacgcggccc acggcgagga gtccgtcgcc gcccgcgagc
     1981 gtgtaggcgc gtgagaggtt catgccgagc agccagtcgg cgcgggagcg tccgcgcgcg
     2041 gcgtcgcgca tgcccgcgta gctgcagttg tcgccgagct tggcgaggcc ttttcggatg
     2101 gaggcggggt ccaccgccga aacccagaat cgctcgacgg gctgtcgggc cctgaagtgc
     2161 tcgagcacct cgtcgacgag gagctgtccc tcgcggtcgg ggtcgcccgc gttgacgacc
     2221 gttgccgcag acttgagcaa tttgccgatc accgagagct gcttcttcgc accgcgctcg
     2281 ctcttcgcct ccatcagcca gtgctgcgga aagatgggca ggtcctgcat gcgccagatc
     2341 ttacgcccct tcttcgtctt cggaatgtcg tcgggcaggt aggcgtcggg ctcggcctgc
     2401 tcgagaagat ggccgaagca ccaggtgacg accacgtcgt ttcggcagtt gatgaacccg
     2461 tcgcctcggc ccgatacgcc gagctcggcg gcgatggcct tggcaacgga gggcttttcg
     2521 gcgatgaaga gcttcatgag ggatccgttc gagattatcg gagcagtgta cagatttcgc
     2581 gctccggacg cacggcccgc tcaaaagagc cttcaatttc gactcacgat tgtatgggga
     2641 a
//
LOCUS       DBMDTI010000284         2612 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8744_length_2612_cov_2.445241, whole genome shotgun sequence.
ACCESSION   DBMDTI010000284 DBMDTI010000000
VERSION     DBMDTI010000284.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8744_length_2612_cov_2.445241"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="ACZPB6_09650"
     CDS             <1..200
                     /locus_tag="ACZPB6_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HIR1184764.1"
                     /translation="GKTERAGYTLVPLDLHFTRGRVKVTVALARGMREYEKRADQSKK
                     DWKREQERIMKKDAHGRHVLE"
     gene            309..1178
                     /locus_tag="ACZPB6_09655"
     CDS             309..1178
                     /locus_tag="ACZPB6_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron protein NifH"
                     /protein_id="HIR1184765.1"
                     /translation="MPTLDSPTLDTVFFDYDRKPLSRHPMTCLAIYGKGGIGKSTTAA
                     NVALALAQDGRRVMLVGCDPKADSTALLRGGAKIETVLSLLRTKHKSELRLDDMVVEG
                     KGGVLCVEAGGPRPGVGCAGRGIIAAFEALRAQDAFNILRPDVVIFDVLGDVVCGGFA
                     MPIRDGWARNVFIITSGENMAIHAAANIATAVETFAERGYARLGGIILNARDVPREAE
                     KVEELACDLNTTIVGRLPRSAEVQEADALGLTVLEHAPSSGMADRYRELARRLVEACA
                     ATRPEPARGGFTF"
     gene            1188..2525
                     /locus_tag="ACZPB6_09660"
     CDS             1188..2525
                     /locus_tag="ACZPB6_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012375.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HIR1184766.1"
                     /translation="MLRLFAHTPERVLPAPTAKLSEAHWPVPFRQGLEYSPPARGPWT
                     IVHVGMLVPECHEIFVCAQGCLRGVVLSAAELGLQRRFSTITVTEEHLRDGQLEDTII
                     EGVTHILERLPKLPPAVLVYSSCLHHFSGADLGWAYRKLSEAFPDVVFTDCYMTPTLR
                     KSEMPPDNKMRRQLYHGLRPADLPKGGLLVAGSLEAFGPESDFARAAHAAGVPFFELP
                     GMTTYEEYRRMAHARWVVTVNPAALQAGRFLAERHGMRHLQLLLDYNEQRIDDSLGTL
                     AELTGIPLWDTTAEKSAARTALAQAADALAGRPVAICQTATTRPVALARRLVESGIRV
                     TDLYCDSFLSADKTDFEILRKKAPGILVHPTTVPEMRFATPAEKRDDIVAIGQKAAFF
                     TGATHMLNMIEGGPWWGHGGVRSLALALAAAAREPVDVDRIISVKGYGCNGCC"
BASE COUNT          435 a          980 c          814 g          383 t
ORIGIN      
        1 tcggcaagac ggagcgcgcg ggctacacgc tcgtgccgct cgacctgcac ttcacccgcg
       61 gccgcgtcaa ggtgacggtc gcgctcgcac gaggcatgcg cgaatatgaa aagcgcgccg
      121 accagagcaa gaaggactgg aagcgcgagc aggagcgcat catgaagaag gacgcccacg
      181 gccgccacgt cctcgaatag cctttccctt cagccagcag gctgcggaca gacagccggc
      241 tgccggcccg atgcgccgcg aagcaggcgc aaaacacgaa ccaacactcc accgggacga
      301 ccatccacat gcccacgctc gactcaccga cgctagatac ggttttcttc gactacgacc
      361 gcaagcccct ttcccggcac ccgatgacct gccttgcgat ctacggcaag ggcggcatcg
      421 gcaagtccac cacggctgcg aacgtggcgc tcgcgcttgc gcaggacggc cgcagggtga
      481 tgctcgtcgg ctgcgacccg aaggccgact cgaccgcgct cctgcgcggc ggcgcgaaga
      541 tcgagaccgt gctctcgctc ctgcgcacga agcacaagtc cgagctcagg ctcgatgaca
      601 tggtggtcga gggcaagggc ggcgtcctct gcgtcgaggc gggcggtccc cgtccaggcg
      661 tgggctgcgc gggccgcggc atcatcgccg ccttcgaggc cctccgggcc caggacgcct
      721 tcaacatcct tcgtcccgac gtcgtgatct tcgacgtgct cggcgacgtc gtctgcggcg
      781 gcttcgcgat gcccatccgc gacggctggg ccaggaacgt cttcatcatc acctcgggcg
      841 agaacatggc cattcatgcg gccgccaaca tcgccacggc cgtcgagacc ttcgccgagc
      901 gaggctacgc ccgcctcggc ggcatcatcc tcaacgcccg cgacgtgccc cgcgaggccg
      961 agaaggtcga ggagctcgcc tgcgacctca acacgacgat cgtgggccgc ctgccccgct
     1021 cggccgaagt gcaggaggcg gacgcgctcg gactcaccgt ccttgagcac gcgccgtcgt
     1081 ccggcatggc cgaccgctat cgcgagcttg cccgccgtct cgtcgaggct tgcgccgcca
     1141 ccaggcccga gcccgcccgc ggcggcttca cgttctgagg cccgcccatg ctccgactct
     1201 tcgcacacac gcccgaacgc gtgcttcccg cacccacggc gaagctctcc gaggcgcact
     1261 ggcccgtgcc cttcaggcag ggcctcgaat actcgccgcc cgcgcgcggg ccctggacca
     1321 tcgtgcacgt gggcatgctc gtgcccgaat gccacgagat cttcgtctgc gcccagggct
     1381 gcctcagggg cgtcgtgctc agcgcagccg aactcggcct gcagcgccgc ttctccacga
     1441 tcaccgtgac cgaggagcac ctgcgcgacg gccagcttga ggacacgatc atcgagggcg
     1501 tcacgcacat tctcgagcgc ctcccgaagc ttccgcccgc agtcctcgtc tactcgagct
     1561 gcctgcacca cttctcgggc gccgacctcg gctgggccta caggaagctc tccgaggcct
     1621 tccccgacgt cgtcttcacc gactgctaca tgacgcccac gctccgcaag agcgagatgc
     1681 cgcccgacaa caagatgcgc aggcagctct accacggcct caggcccgcc gaccttccca
     1741 agggcggcct gcttgtggcg ggctccctcg aggccttcgg ccccgagagt gacttcgcgc
     1801 gcgccgcaca cgcggcgggc gtcccgttct tcgagctccc gggcatgacg acctacgagg
     1861 aataccgccg catggcgcat gcgcgctggg tcgtgacggt caatcccgcg gcccttcagg
     1921 cgggcaggtt cctcgccgag cgccacggca tgcgccacct tcagctgctg ctcgactaca
     1981 acgagcagcg catcgacgac tcgctcggaa cgctcgccga actcacgggc attccgctct
     2041 gggacaccac ggccgagaag tccgctgcca ggaccgcgct cgcgcaggcg gccgacgcgc
     2101 tcgcgggccg tcccgtggcg atctgccaga cggcgacgac gcgccccgtg gcgctcgcac
     2161 gccgcctcgt cgagtcgggc atccgcgtca cggacctcta ctgcgacagc ttcctgtctg
     2221 cggacaagac cgacttcgag attcttcgca agaaggcgcc cggcattctc gtgcatccga
     2281 cgacggtgcc cgagatgcgc ttcgcgacgc ccgccgaaaa gcgcgacgac atcgtggcca
     2341 tcggccagaa ggccgccttc ttcaccgggg cgacccacat gctcaacatg atcgagggcg
     2401 gcccctggtg gggtcacggc ggcgtgagaa gcctcgcgct cgcgcttgcg gctgcggcac
     2461 gcgagcccgt cgacgtcgac cgcatcatca gcgtcaaggg atacgggtgc aacggatgtt
     2521 gttagactac acgcaaagct ccatgtcgac ctacacggcc gactgctcgg gcgtcgcaag
     2581 cgccctctat gaactcggcg gcatgaccgt ca
//
LOCUS       DBMDTI010000285         2604 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8777_length_2604_cov_1.928486, whole genome shotgun sequence.
ACCESSION   DBMDTI010000285 DBMDTI010000000
VERSION     DBMDTI010000285.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8777_length_2604_cov_1.928486"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="ACZPB6_09665"
     CDS             complement(<1..388)
                     /locus_tag="ACZPB6_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184767.1"
                     /translation="MQTSRRKLLLTGAAAGAGLTTSAFAKAAPPKFDEVYDVVVVGSG
                     IAGTMAALVAAKNGAKTLLIEKLNRLGGTSRYSGLDFACVGSDMQKAAGITETPEAMV
                     ADMAKVAANLSDRARALNIARETARAQ"
     gene            complement(498..1226)
                     /locus_tag="ACZPB6_09670"
     CDS             complement(498..1226)
                     /locus_tag="ACZPB6_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1184768.1"
                     /translation="MTDKPLILVADDETRIRRLVVESLENAGFDVCQAQDGRQAVDVF
                     RLSPVEPDLVILDLMMPEMDGEEALKAIRKTSSVPVLILTARDFLCDKSKCFNAGADD
                     YLVKPFSIQELELRVRALLRRMARQFMVPRGRTVVNGPLTLRSEEHDVLWRGTSVPVT
                     DREFRLLESLASRPGAVVRYDELLRIGWGLDSDADISHLRVAVARIRKKLESISVNPA
                     IISSFTNVGYMMGDLTSYDEDYGD"
     gene            complement(1223..>2604)
                     /locus_tag="ACZPB6_09675"
     CDS             complement(1223..>2604)
                     /locus_tag="ACZPB6_09675"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR1184769.1"
                     /translation="PVYMTSQKLHAGLPQWTVVVTRPEKSRMMVRTNLEVRMAFLALG
                     VLLLTSAAGWLACWPLSNALRKLRGDIDEDYGDDRNTIEQGPEELREVQAAYRELRKR
                     LHARQVSLEEKNVHLASTVVRRESELDAQERLFREVFEDMKDGIVLLDPQWRPQLENT
                     SAASMMPGSFKHEMLTFCRRQFEAGEQDVAVYEAELDGAMKAWECRTFPFRAGRAPFA
                     EGFCVICRDVTERQEVERMKNDLIGIVAHELKTPVTACRLQIDLMRKASGDTEELKAL
                     DSDLDHLNHIVSDWLSAAKIEGGNFSVHPKATQIGPLIRRAVRLVRSRHEFDFKMRVA
                     EEAECLNVDPGAFVELVVNLVTNACRYVRPSERPEISFEVEAVQPWAVIRVKDHGIGF
                     DPSLSERIFDRFYQVVSGRKRRTGGTGLGLVICRAICEAHGGSIKASCAEGETCFTIH
                     LPLPRSIES"
BASE COUNT          547 a          804 c          702 g          551 t
ORIGIN      
        1 tctgagcgcg ggccgtttca cgagcaatgt tgagtgcacg tgcacgatcg ctgagattgg
       61 cagcaacctt cgccatgtcg gccaccatgg cctcgggcgt ctcggtgatg cctgccgcct
      121 tctgcatgtc gctgccgacg caggcgaagt ccagaccgct gtagcgtgac gtgccgccca
      181 ggcgattgag cttttcaatg agaagggttt tggcgccgtt cttcgcggca accagagccg
      241 ccatcgtgcc tgcgatgccc gagccaacga caacaacgtc gtagacttcg tcaaacttgg
      301 ggggcgccgc ctttgcaaag gcggatgtcg taagtcccgc accggcggcc gcgcccgtga
      361 ggaggagttt gcgtcttgag gtctgcatga tcggactctc catgtgtggt ctgagttgac
      421 tcacatggta ggtcccgaag attgcccgat gattgccgac cactcaggat tatttcaaaa
      481 agattgcagc ccctctttca gtcgccgtaa tcttcgtcgt aactggtcag gtctcccatc
      541 atgtagccca cattggtgaa ggaggagatg atggcgggat tgactgaaat gctctcgagc
      601 ttctttcgga ttcgggccac ggccactcga agatgggaga tgtcggcatc cgaatcaagc
      661 ccccagccga tgcgcagcag ttcgtcatag cgtacgactg ctccaggtcg ggacgcgaga
      721 ctctcaagca gcctgaactc gcgatccgtc acgggaacac tcgttcctcg ccagaggacg
      781 tcatgttcct cggacctgag cgtcagagga ccgttgacga ccgttcgccc tcggggcacc
      841 atgaactgcc tcgccatcct tcgcagcaag gccctcacac gcagttcgag ctcctgaatg
      901 gaaaacggct tgacgagata gtcatctgct cccgcattga agcacttgct cttgtcacag
      961 agaaaatctc tggccgtcag aataaggact ggcactgaac tcgtcttccg aatggccttg
     1021 agcgcctcct cgccgtccat ttcaggcatc atgaggtcga gaatcaccag atcaggttcc
     1081 acaggagaaa gcctgaagac atcgacagcc tgtcgtccgt cctgcgcctg acatacgtca
     1141 aaaccggcgt tttcgagcga ttcaactaca agccttcgga tgcgggtttc gtcgtcggca
     1201 acaagaataa gcggtttgtc ggtcatgatt caatgctcct ggggagcggc agatgaatgg
     1261 taaaacacgt ttcgccttcg gcgcaggaag ccttgattga acccccgtga gcttcgcaga
     1321 tcgcgcgaca gatgacaagt ccgaggcccg taccgcccgt acgacgcttt cgtccgctca
     1381 ccacctgata gaagcggtcg aagatgcgct cggaaagcga cggatcaaac ccaatgccat
     1441 gatccttgac gcgaataaca gcccacggct ggaccgcctc gacttcgaac gaaatctcgg
     1501 gcctttcgct gggcctgaca tatcgacagg cgttggtgac aagattgacc acgagctcca
     1561 cgaaggcgcc aggatccacg ttgaggcact ccgcctcctc ggccaccctc attttaaagt
     1621 cgaactcatg tcggcttctg acaagtctta cggcacgccg gatgagcggc ccgatctgtg
     1681 ttgccttcgg atgaacggaa aagtttccgc cctcgatctt tgccgccgac agccagtcgc
     1741 tcacgatgtg attgagatga tcaaggtcgg agtcgagcgc cttgagttcc tcggtgtcgc
     1801 ccgaggcctt tctcatgaga tcgatctgaa gcctgcaggc cgtcacaggc gtcttgagtt
     1861 catgagcaac aatgccgatc agatcattct tcatgcgctc cacttcctgg cgctccgtga
     1921 catccctaca gatgacgcag aagccctcgg caaaaggcgc acgtcctgcc ctgaagggaa
     1981 aggttcggca ttcccacgcc ttcattgcgc cgtcaagctc cgcctcgtag acggcgacat
     2041 cctgttcccc cgcctcaaac tgtctgcgac agaaagtcag catctcatgc ttgaaagacc
     2101 cgggcatcat cgaggccgcg gaggtgtttt caagttgcgg ccgccactga gggtcgagaa
     2161 gaacgatccc gtccttcatg tcctcgaaaa cctccctgaa gagacgctcc tgcgcatcga
     2221 gctccgattc acgccttaca accgtgctcg ccagatggac attcttctcc tccagagaaa
     2281 cctgccttgc atgcagtctc ttgcgcagct cgcgatatgc ggcctgcact tcgcgaagtt
     2341 cctcaggtcc ctgctcaatc gtgttcctgt cgtccccgta gtcctcatca atgtctcccc
     2401 tgagcttccg aagagcgttt gaaagcggcc aacatgcaag ccagcctgcc gccgacgtca
     2461 ggagcagtac gccaagcgcc agaaaagcca tcctcacctc gagattcgta cggaccatca
     2521 tcctgctctt ctcggggcga gtaaccacga ccgtccattg cggaagcccc gcatgaagct
     2581 tctggctcgt catgtatacg ggac
//
LOCUS       DBMDTI010000286         2565 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8949_length_2565_cov_3.327110, whole genome shotgun sequence.
ACCESSION   DBMDTI010000286 DBMDTI010000000
VERSION     DBMDTI010000286.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8949_length_2565_cov_3.327110"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="ACZPB6_09680"
     CDS             <1..702
                     /locus_tag="ACZPB6_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HIR1184770.1"
                     /translation="GNSALMWDLSVDPLIVTKLSDEEIKSRLFARREDLADGEEPLPV
                     YRLAMNNSPFVCASLKVLSPERMDRFGVKPQEILANWERFKELGNVIAGRLAGLMGRR
                     ERPAPKDVDFGLYDSGFASGHDVETMARVRGMTSESLAKGGIHFDDERFNQLLVRYRA
                     RNWQETLNDEERSQWTDFCRSRLMDGADGALTVSAYFDEIDRLQESTLEDEEKQLVLE
                     ALYEWGERAGEACAF"
     gene            726..1628
                     /locus_tag="ACZPB6_09685"
     CDS             726..1628
                     /locus_tag="ACZPB6_09685"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1184771.1"
                     /translation="MKMDKTYACVPSLKVAFVGLGVMGFPMAGHLKLAGHDVCVYNRT
                     TAKAEKWVETFGGRMAATPAEAVRGAGIVFACVGNDDDLRSVVLGETGAFAGMEPGTV
                     FVDHTTASATVARELEAEGARRKIPFIDAPVSGGQTGAENGCLTVLCGGDEAVFRRVE
                     PVLDAYAGAVTYFGPSGSGQLAKMVNQICIAGCVQGLSEAIAFGMNAGLDMPKVIEAL
                     SSGAASSWQMMNRGRTMVKGEFDFGFAVDWMRKDLGIVKDEAARNGSEIPVTRMVDGF
                     YEEVQKAGGARWDTSSLITRLPRP"
     gene            1721..>2565
                     /locus_tag="ACZPB6_09690"
     CDS             1721..>2565
                     /locus_tag="ACZPB6_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HIR1184772.1"
                     /translation="MSSETEAPVAEAQVTNFIRNIIDDDLARGANLPRFWCGHPAPYA
                     EQLEKGEPDAARIRTRFPPEPNGYLHIGHAKSVCLNFGLARDYGGACHMRFDDTNPVK
                     EDQEYVDGIMDSVRWLGFDWKFGNETNLYFASSYFDDMFRFAEHLIRTGYAYVDEQTA
                     DEMRENRGTLKEPGKNSPYRDRSAEENLKLFHEMRDGKHPEGSMVVRAKIDMASPNIN
                     LRDPAIYRIRFADHHATGDKWCVYPMYTFAHPIEDSLENITHSICTLEFEDQRAFYNW
                     ALERI"
BASE COUNT          531 a          714 c          814 g          506 t
ORIGIN      
        1 ggcaattccg cgctgatgtg ggacttgtcc gtggatccgc tgatcgtgac gaagctgagc
       61 gacgaagaga tcaagtcacg ccttttcgcc cgcagggagg atcttgcgga cggcgaggag
      121 ccgttgccgg tctacaggct cgcgatgaac aactctccct ttgtctgcgc aagcctcaag
      181 gtgctttctc ccgagcgcat ggacaggttc ggcgtgaagc cgcaggagat tcttgccaac
      241 tgggagcgct tcaaggagct cggcaacgtg attgcgggaa ggctggccgg actgatgggc
      301 aggcgtgaaa ggcccgcgcc gaaggacgtt gacttcggac tttatgacag cggctttgcc
      361 agcggacatg atgtggagac gatggctcgg gtgcgcggaa tgacgtctga gagtcttgcg
      421 aagggcggca ttcattttga cgatgaacgc ttcaaccagc tgcttgtgcg ctaccgcgcc
      481 cgaaactggc aggagacgct caatgatgaa gaacgcagcc agtggacgga tttttgccgt
      541 tccagattga tggacggagc cgacggcgct ctgaccgttt cggcctattt tgatgaaatc
      601 gaccgcctgc aggagagcac tttggaagat gaagaaaagc agctcgtgtt ggaggcgctt
      661 tacgaatggg gcgagcgtgc gggtgaagcc tgcgcgttct gagtctgttg aagaaggaga
      721 cggttatgaa gatggacaag acttatgcgt gcgttccctc cctcaaggtg gcgtttgtcg
      781 gcctaggcgt catgggattt ccgatggcgg ggcatctgaa gctagccgga catgacgtct
      841 gtgtctacaa ccgtacgacg gccaaggctg agaagtgggt tgagacgttc ggaggccgca
      901 tggcggcgac gccggctgaa gcggtccgcg gagcaggtat cgtcttcgcc tgcgtgggca
      961 acgacgacga tctgagaagc gtcgtgcttg gagaaacggg ggcgtttgcc ggcatggagc
     1021 cagggacggt cttcgtcgac catacgacgg caagcgctac cgtggcacgc gagcttgagg
     1081 ccgaaggagc gaggaggaaa attcccttca tcgacgcacc ggtctccggt ggtcagactg
     1141 gcgctgaaaa cgggtgcctg acggtgctct gcggcggaga cgaggctgta ttcaggcgcg
     1201 ttgagcctgt gcttgacgca tatgcgggcg cggtgactta cttcggaccg tctggttcag
     1261 gtcagctcgc caagatggtc aaccagatct gcattgcagg ctgtgtacag ggactctccg
     1321 aagctatcgc ctttggcatg aatgccgggc ttgacatgcc gaaggtcatc gaggccctga
     1381 gctcaggcgc cgcatcttcc tggcagatga tgaaccgcgg caggacgatg gtgaagggtg
     1441 agttcgactt cgggtttgct gtcgactgga tgcgcaagga tcttggcatt gtcaaggatg
     1501 aggcggcccg caacggctcg gagattcccg tcacgaggat ggtcgacggc ttctacgaag
     1561 aggttcagaa ggcgggcggc gcccgctggg atacgtcaag cctcattaca cgccttccca
     1621 ggccgtaggc gacggggaag tgacgaatcc gcgttcgtca gcctcccggg gtttgcggct
     1681 acaatacaca gattaccttc attgaattcg gattagatca atgtcttctg aaaccgaagc
     1741 acctgtcgcc gaagcgcagg tcaccaactt tattcgaaac atcatcgacg acgatctggc
     1801 ccgcggcgcc aacctgcccc gattctggtg cgggcatccc gcgccttatg ccgagcagct
     1861 cgaaaagggc gagcccgacg cggcccgcat ccgcacgcgc ttcccgccgg agcccaacgg
     1921 ctatctgcac atcggccatg ccaagagcgt ctgtctgaac ttcggccttg ctcgtgacta
     1981 cggcggcgcc tgccatatgc gctttgacga caccaatccc gtcaaggagg atcaggaata
     2041 cgtcgacggc atcatggaca gcgtccgctg gctcggcttc gactggaagt tcggcaatga
     2101 aacgaacctc tactttgcga gctcgtactt tgacgacatg ttccgctttg ccgagcatct
     2161 gatccgcacg ggctatgcct atgtggatga acagactgca gacgaaatgc gcgaaaaccg
     2221 cggcacgctc aaggaaccgg gcaagaattc gccctaccgc gaccgctcgg ccgaggagaa
     2281 tctgaagctc ttccatgaaa tgcgcgacgg caagcatccc gaaggctcca tggtggttcg
     2341 cgccaagatc gacatggcga gccccaacat caatctgcgc gaccccgcca tctaccgcat
     2401 ccgctttgcc gatcatcatg cgacgggcga caagtggtgc gtgtatccga tgtacacctt
     2461 cgcgcacccg atcgaggact cgctcgaaaa catcacgcac tccatctgca cgctggagtt
     2521 cgaggatcag cgcgcgttct acaactgggc tcttgagcgc atcat
//
LOCUS       DBMDTI010000287         2562 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_8963_length_2562_cov_2.994718, whole genome shotgun sequence.
ACCESSION   DBMDTI010000287 DBMDTI010000000
VERSION     DBMDTI010000287.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8963_length_2562_cov_2.994718"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(46..258)
                     /locus_tag="ACZPB6_09695"
     CDS             complement(46..258)
                     /locus_tag="ACZPB6_09695"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR1184773.1"
                     /translation="MTKALILASSSRYRRELLDRLGIAYTCESPDIDESSLPGETPQD
                     TAMRLSEMKARAIWDKHPGAVVIGSD"
     gene            380..880
                     /locus_tag="ACZPB6_09700"
     CDS             380..880
                     /locus_tag="ACZPB6_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014657.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR1184774.1"
                     /translation="MSLTLDVFEIARTRQTVEGTLELDEMPELAHFIQALEGSLKFSV
                     TGLGIVERLPAASLSFEGTVVMQCARCNEPVSVPISRELVFRFTKTEAEANALPLDEE
                     GDDEEVIVGSRSLSVADWVQEEAILSLPAMPLHDDCEMDYENTSDEVVEAEKPNPFAA
                     LAALKH"
     gene            complement(1138..>2562)
                     /locus_tag="ACZPB6_09705"
     CDS             complement(1138..>2562)
                     /locus_tag="ACZPB6_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184775.1"
                     /translation="LADDAEAFDRDAAAESGTLADMIRELAGTLGDDSNPLVVSLDVI
                     WGAISSSSIVTGADPDDDEDPIEHYLYDNSDLTGLMGAYWDLQGVLEIMFGELGMNAD
                     FRGSLIELPTEAASVAELNKSLQRLSSSSRACFAWCHNRIVSVLRDLSNDDPKVLEKN
                     LESSGIQPLFIPAVLRQFNESRTASDVLAAVSSRFSPFDRELDAVFEQFWNLQRAVSQ
                     KADHPARVALALLVSALAAMLDCRPEDIDSRASSRLAESDDLAASDRGLALHLAGIVA
                     GVELVESDDPVMQMCRSFDEACESAEANQDICLPLSCALTAVNHFLKDEAAAKEHFKK
                     LHLADATTETFLPSLATLSEFLVHHTPEKDDGSLLQKHFAPVIEGDYDGPTAGEHYAK
                     LLSETLSRMDEESRALFTASFLTLGYLMSGIFARTPEEIEEFFKQAFPGFIVDPIIAM
                     CMSILPAASKLAVEATEKMEAATA"
BASE COUNT          476 a          718 c          872 g          496 t
ORIGIN      
        1 gtgcgtgtgc ggcttgccga gcttgacgcc gttgaggtcg gccacctaat cggagccgat
       61 gacgacggcg ccggggtgct tgtcccagat tgcgcgggcc ttcatttcgg aaaggcgcat
      121 cgcggtgtcc tgcggcgttt cgccgggcag ggaggattcg tcaatgtcgg ggctttcgca
      181 cgtgtaggcg atgccaagac ggtcgaggag ctcgcggcgg tagcgggagc tcgaggcgag
      241 gatgagtgct tttgtcataa tgcaggaatt gtagcgggaa gtcccaaggc gggcgagcga
      301 aattcccgcc gcctgcgcgt gaacaatgta tcctttcagg gcgcccgttc cgggcctgaa
      361 agttttactg aggatgacca tgtctcttac gctcgatgtt ttcgaaatcg cccgcacccg
      421 ccagaccgtg gagggcacgc ttgagcttga cgaaatgcct gagctcgcgc acttcattca
      481 ggcgctcgag ggctccctga agttcagcgt gacgggactc ggcatcgttg agcgacttcc
      541 cgccgcaagt ctctcgttcg agggcacggt cgtcatgcag tgcgcgcgct gcaacgagcc
      601 cgtgagcgtt cccatcagcc gcgaactcgt cttccgcttc acgaagaccg aggcagaggc
      661 caatgcgctc ccgctcgatg aagagggtga tgatgaggag gttatcgtcg gcagccgcag
      721 tctctccgtc gccgactggg ttcaggagga ggcgatcctg tcgcttcccg ccatgccgct
      781 tcacgacgac tgcgagatgg actatgaaaa cacgagcgac gaggtggtcg aggctgaaaa
      841 gcccaatccc ttcgccgctc tcgccgcgct caagcactga gcccgaacac cacgccttgt
      901 gaagacgccc gcccggcatt tgattcggag cgggtttttc cttgtctttt cgggcttagg
      961 ctgagcggcg tcggacgcgg tcgcaagcga ggccatgagc atggaatgca cagaccggcc
     1021 gaaatctcca gcaggccccg tcgtcggttc tggtcctgaa taaaaatcgc ccggctcaca
     1081 caagccgggc gacggatgcg tctgatgacg ttggcgatga cgacggcttt ggagacgtca
     1141 ggcggtggcg gcttccatct tttccgtggc ctcgaccgcg agcttggagg cggcgggcag
     1201 gatggacatg cacatggcga tgatcggatc gacgatgaat ccggggaacg cctgcttgaa
     1261 gaattcctcg atttcctcag gcgtgcgggc gaagatgccg ctcatcaggt agcccagcgt
     1321 gaggaaagac gccgtgaaaa gagcgcggct ctcttcgtcc atcctggaaa gtgtctcgct
     1381 gagaagcttc gcgtagtgtt cgcctgcagt ggggccatcg taatcgcctt cgatgacggg
     1441 ggcgaagtgc ttctggagaa gggagccgtc gtccttctcg ggtgtgtgat gcacgaggaa
     1501 ttccgaaagc gtggcaaggc tgggaaggaa ggtttcggta gtggcgtctg ccagatggag
     1561 cttcttgaag tgttcctttg cggcggcctc gtccttcagg aaatgattga cggcggtgag
     1621 tgcgcatgag agcggaagac agatgtcctg attcgcttcg gcactttcgc atgcctcgtc
     1681 gaagctcctg cacatctgca tcacggggtc gtcggattcg acgagttcca cgccggcgac
     1741 gatgccggcc agatggaggg cgagtccccg gtcggaggcc gcaagatcgt cagactccgc
     1801 gagacgggag ctcgcgcggg agtcgatgtc ttcgggacga cagtcgagca tggcggccag
     1861 ggcggagacg agaagggcaa gagcgactct ggcgggatgg tcggccttct ggctcacggc
     1921 gcgctgaagg ttccagaact gctcgaaaac ggcatcgagc tctcggtcga agggcgagaa
     1981 gcggctggag actgcggcga gcacgtcgct tgccgtgcgg gactcgttga actgcctgag
     2041 cacggccgga atgaaaagcg gctgaatgcc ggaactctca aggtttttct cgagaacctt
     2101 cgggtcgtcg ttcgagaggt ctcgaagcac cgagacgatc ctgttgtggc accacgcaaa
     2161 gcaggcgcgg gaactcgacg aaaggcgctg caggctcttg ttgagctcgg cgacgctagc
     2221 ggcctcggtc ggaagttcga tgaggctgcc ccggaagtcg gcgttcatgc cgagttcgcc
     2281 gaacatgatc tcgagaaccc cctgaaggtc ccagtaggcg cccatgagcc ccgtgaggtc
     2341 gctgttgtcg tagagatagt gctcgatggg atcctcatcg tcgtcgggat ccgcgccggt
     2401 gacgatggac gaggaggaaa tcgcgcccca gatgacgtcg agcgagacga cgagcggatt
     2461 gctgtcgtct ccgagcgttc ccgcgagctc gcgaatcatg tctgcgaggg ttccgctctc
     2521 ggcggccgcg tcacggtcga aggcttccgc atcgtcggca ag
//
LOCUS       DBMDTI010000288         2531 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9086_length_2531_cov_2.375309, whole genome shotgun sequence.
ACCESSION   DBMDTI010000288 DBMDTI010000000
VERSION     DBMDTI010000288.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9086_length_2531_cov_2.375309"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="ACZPB6_09710"
     CDS             <1..773
                     /locus_tag="ACZPB6_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1184776.1"
                     /translation="RMSGAPLAGMFMAVIPVLVAIVANVRYQREGRGLSWGSITPPLV
                     LIFFGLVIANWTEFQYITSSGSTGLDFWIGVLFGIAAVISWTWFSIMNGEWLLAHPKH
                     SSSAWTALQGVTVLPVVMIAFAILAWPCGWMDTSVSLLGETPLRFLGVALMIGLLCSW
                     VAMLCWNQMSQRLPSGLGGQLIVFESISAVVYALIWRGEWPTITMVIGFTILLVGVMC
                     SLYVFGKSPAKKEDESKKACAAPSKAAKPEEKLEGEAA"
     gene            complement(872..1669)
                     /locus_tag="ACZPB6_09715"
     CDS             complement(872..1669)
                     /locus_tag="ACZPB6_09715"
                     /inference="COORDINATES: protein motif:HMM:NF019322.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1184777.1"
                     /translation="MTVKHKNEETHMLRKTGRRLAVARCPLYEQVRNVLVERLRHGDW
                     HPGDRLPTESVLAEELGVSLGTLRRAVELLVEEGALIRRQGAGTFAATLATVAEENRF
                     QPFESLDGSTRFDFRRLVLLEEAPCPAEAAAGLGIRAGDTVIHMVRHMVKRTPEGERI
                     AASDEIFLLPEFFPGLTAERYRRGYRPDDSIYRFYAREMGVVITSQRCAVRYECVKGS
                     EAVRLAVPEPMPVLRFARISMAFGHLPVEYRIYRLDAENSQICFALP"
     gene            1887..>2531
                     /locus_tag="ACZPB6_09720"
     CDS             1887..>2531
                     /locus_tag="ACZPB6_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184778.1"
                     /translation="MNFRTLFTPAALAGLLALPGAASALDAIPASCLKCRQAPDDTAF
                     ITCLYECLADLKTAPGASSGDASKSKAGTPSTDSGKSGKKTQADGLPAFEGGWTVYDR
                     PDAAEGDQARSAELLSRSAVSAFFQRVQPVLVLAKEKKGDVHAFLNLEPAVVGSFSDR
                     VLVQFDEGRPRALPLRALMGGSAVLLQDKTFLKDLASARKLYVRLDLFGAPAQTM"
BASE COUNT          409 a          931 c          714 g          477 t
ORIGIN      
        1 tccgcatgtc gggcgctccg cttgcgggca tgttcatggc cgtcattccc gtgctcgtcg
       61 ccatcgtggc gaacgtgcgc tatcagcgcg agggccgcgg cctctcgtgg ggctccatca
      121 cgccgccgct cgtgctcatc ttcttcggcc tcgtgatcgc caactggacc gaattccagt
      181 acatcacctc ctcgggctcc acgggcctcg acttctggat cggcgtgctt ttcggcatcg
      241 cggccgtgat ctcctggacc tggttctcca tcatgaacgg cgaatggctt ctcgcccacc
      301 cgaagcacag ctcgtccgcc tggacggccc tgcagggcgt cacggtgctc cccgtcgtca
      361 tgatcgcctt cgcgatcctc gcctggccgt gcggctggat ggacacctcg gtgtcgcttc
      421 tcggtgaaac gccgctgcgc ttcctcggcg tggccctcat gatcggcctg ctctgcagct
      481 gggtcgccat gctctgttgg aaccagatgt cccagcgcct gccctccggc ctcggcggcc
      541 agctcatcgt cttcgagagc atctccgccg tcgtctacgc cctcatctgg cgcggcgaat
      601 ggcccaccat cacgatggtc atcggcttca cgatcctgct cgtgggcgtg atgtgctccc
      661 tctacgtctt cggcaagtcc cccgccaaga aggaggacga atcaaaaaaa gcgtgcgcgg
      721 ccccgtcgaa ggccgcgaag cctgaggaaa agcttgaggg cgaggccgcg taaggccccg
      781 ccgcgaaaag accttcgaca ttgacaaacg gcccggtcgc ctgtatggcg tccgggccgt
      841 tttccattcc ccccttcaga aacgtgcctt cctacggcag cgcgaagcag atctgcgaat
      901 tctccgcatc gagcctgtag atgcggtact cgacgggcag gtgcccgaag gccatcgaga
      961 tgcgcgcaaa cctcagcacg ggcatcggct cgggaacggc gagccgcacg gcctcgctcc
     1021 ccttcacgca ctcgtacctc accgcgcagc gctggctcgt gatcaccacg cccatttcgc
     1081 gcgcatagaa cctgtaaatc gagtcgtcgg gcctgtagcc cctgcggtag cgctcggccg
     1141 tgagtccggg gaagaattcg ggcagcagga agatctcgtc cgaggccgca atgcgctcgc
     1201 cctcgggcgt gcgcttgacc atgtgcctca ccatgtggat caccgtgtcg cccgcccgga
     1261 tcccgagccc cgccgcagcc tccgccggac agggcgcctc ctcaagcaag acgagccgcc
     1321 tgaagtcgaa acgcgtgctg ccgtccaaac tctcgaaggg ctggaacctg ttctcctcgg
     1381 ccaccgtcgc aagggtcgcc gcaaacgtgc ccgcaccctg cctgcggatg agcgcacctt
     1441 cctcaaccag aagctccacg gctcgcctga gcgtgccgag cgatacgccg agctcctcgg
     1501 caagcaccga ctccgtcgga agcctgtcgc cgggatgcca gtccccatgc cgaagccttt
     1561 cgaccagcac attcctcacc tgctcgtaga gcgggcatct cgcaacggca agacgcctcc
     1621 ccgtcttcct cagcatgtgc gtttcctcat tcttgtgttt cacggtcatt tgcgcctcct
     1681 ctactcctgc aggagtagaa attcattcat attgtagatg acccatatgc aactccgcca
     1741 ccgaatgccc cgcggcgtgc cggccgcgtc atgcgtttcc caacggtcag gtctgtcagc
     1801 attcgttacg cgcacgctcg ggcgcgacgc cgcgtgctta gaatgacctc actcgcaatg
     1861 cgaacgactc aactttccgc atcatcatga acttcaggac gcttttcaca cccgccgccc
     1921 ttgcgggcct tcttgctctt ccgggcgccg cctcggcgct cgacgccatt cccgcctcct
     1981 gtctcaagtg caggcaggcc ccggacgaca ccgccttcat cacctgcctc tacgaatgtc
     2041 tcgcggatct gaagaccgca cccggcgctt cctcgggcga cgcttcgaag tcgaaggccg
     2101 ggacgccttc gaccgattcc ggaaagtcgg gcaaaaagac gcaggccgac ggccttcccg
     2161 cctttgaagg cggctggacg gtctacgaca gacccgacgc cgccgagggc gatcaggccc
     2221 gcagcgccga gctcctgagc cgcagcgccg tctccgcctt cttccagcgc gtgcagcccg
     2281 ttctcgtcct cgccaaggaa aagaagggcg acgtccatgc cttcctcaac ctcgagcccg
     2341 ccgtcgtggg ctccttctcg gatcgcgtcc tcgttcagtt cgacgagggc aggccccgcg
     2401 ccctgccgct ccgcgccctg atgggcggca gcgccgtcct tcttcaggac aagaccttcc
     2461 tcaaggacct tgcgtccgca aggaagctct acgtccgcct cgacctcttc ggcgcgcccg
     2521 cccagaccat g
//
LOCUS       DBMDTI010000289         2525 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9114_length_2525_cov_2.401403, whole genome shotgun sequence.
ACCESSION   DBMDTI010000289 DBMDTI010000000
VERSION     DBMDTI010000289.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9114_length_2525_cov_2.401403"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2085
                     /locus_tag="ACZPB6_09725"
     CDS             <1..2085
                     /locus_tag="ACZPB6_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR1184779.1"
                     /translation="MYLFLQNFRATLIPTIAVPVVLLGTFGALFVCGFSINMLTMFAM
                     VLAIGLLVDDAIVVVENVERVMREDGTDPKTATIKSMGEIQSALVGIAMVLSAVFVPM
                     AFFGGSTGVIYRQFSVTIVSAMALSVVVALTLTPALCATMLKPVNHEEHHGKGGFFGW
                     FNRSFDAFTQSVKALIAKSIHGWSVMLVAYAAVVGLMVWGFKALPTSFMPQEDQGVLM
                     MMSQLPDGATLERTDLEMKKIQQTFAKTEAKSVESVFVVRGFSFAGTGQNMALGFLKL
                     RDWSERTTPDTSVAAIQGRTMGILMGHPDFKQASTFVFPLPPVPELGVADGFDLYIQD
                     NTGGTHDKLMQYMGQFLMKANADPRLTMVRHNGMADTTQLKLVLDYEKLSALNLSIGE
                     VNSAISTVLSGSYVNDYMDRGRVKQVWVMGNTGSRMQPEDILGWHVRNANGEMVPIGT
                     FAKFEWSYGSPRLERFNGLPAVNIQGNPRAGVSSGQAMAAIEEIAKEVFPTGYEIAWN
                     GVSYQEKAAGNQGPALFALSILVVFLCLAALYESWSVPIAVLLVVPCGVVGALGLSGA
                     LGMSNDIYFQVGLLTTIGLVSKNAILIVEFAKEYMEQGKDAIHAAEEAVRVRLRPILM
                     TSLAFGLGVLPLAMAHGAGAGAQNAIGVGVLGGMLAGTFLCIAFVPVFYVLVDRFLGG
                     KKRHEKNQTQQA"
     gene            2168..>2525
                     /locus_tag="ACZPB6_09730"
     CDS             2168..>2525
                     /locus_tag="ACZPB6_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR1184780.1"
                     /translation="MSTTIFKILPLCAALALTGCVNLAPDHERPAAPVEQAWPTGEAY
                     DAASVRSEALPEWQAFFTDERLRKVIDLALKENRDLRVSALNIEKARALYGVQRSELF
                     PTVAAAASNAGKHTPAS"
BASE COUNT          438 a          907 c          725 g          455 t
ORIGIN      
        1 atgtacctct tcctgcagaa cttccgcgcg acgctcattc cgacgatcgc cgtgccggtc
       61 gtgcttctgg gcacgttcgg cgcactcttc gtctgcggat tctccatcaa catgctgacg
      121 atgttcgcca tggtgctcgc catcggtcta ttggtggacg acgccatcgt ggtcgtggag
      181 aacgtcgagc gcgtgatgcg cgaggacggc accgacccga agaccgctac catcaagtcg
      241 atgggtgaaa tccagtcggc cctcgtgggc atcgccatgg tcctttcggc cgtgttcgtg
      301 ccgatggcct tcttcggcgg ctccacgggc gtcatctacc gccagttctc ggtgaccatc
      361 gtctcggcca tggcgctttc cgtcgtcgtc gccctgacgc tgacgccggc cctctgcgcc
      421 acgatgctca agccggtcaa ccatgaggag caccacggca agggcggctt cttcggctgg
      481 ttcaaccgca gcttcgacgc cttcacacag tccgtcaagg ccctcatcgc caagtcgatc
      541 cacggctgga gcgtcatgct cgtcgcctac gccgccgtcg tcggcctgat ggtctggggc
      601 ttcaaggcgc tgccgacctc gttcatgccg caggaagacc agggcgtcct catgatgatg
      661 tcccagctcc ccgacggcgc gacgctcgag cgcaccgacc ttgagatgaa gaagatccag
      721 cagacgttcg ccaagaccga agccaagtcc gtcgagtccg tcttcgtggt ccgcggcttc
      781 tccttcgcag gtacgggcca gaacatggct ctgggcttcc tgaagcttcg cgactggagc
      841 gagcgcacga cgcccgacac gtcggtcgcc gccatccagg gccgcaccat gggcatcctc
      901 atgggccacc cggacttcaa gcaggccagc actttcgtct tcccgcttcc gccggttccc
      961 gaactcggcg tggccgacgg cttcgacctc tacatccagg acaacacggg cggcacgcac
     1021 gacaagctca tgcagtacat gggccagttc ctgatgaagg ccaatgccga cccgcgtctg
     1081 acgatggtcc gccacaacgg catggccgac acgacgcagt tgaagcttgt gctcgactat
     1141 gaaaagctct cggccctcaa cctcagcatc ggcgaagtca actccgccat ctcgaccgtc
     1201 ctctccggct cctacgtcaa cgactacatg gaccgcggcc gcgtcaagca ggtctgggtc
     1261 atgggcaaca cgggctcgcg catgcagccc gaagacatcc tcggctggca cgttcgcaat
     1321 gccaacggcg agatggtgcc catcggcacg ttcgccaagt tcgaatggtc ctacggcagc
     1381 ccgcgtctcg aacgcttcaa cggccttccc gccgtgaaca tccagggcaa cccgcgtgcg
     1441 ggcgtctcct ccggtcaggc catggccgcc attgaggaaa tcgccaagga agtcttcccc
     1501 accggctacg aaatcgcttg gaacggcgtt tcgtaccagg aaaaggccgc aggcaaccag
     1561 ggtcccgcgc tcttcgcgct ctcgatcctc gtggtgttcc tctgccttgc cgccctttat
     1621 gaaagctggt ccgtcccgat cgcggtgctc ctcgtggtgc cctgcggcgt cgtgggcgcc
     1681 ctcggcctca gcggcgcgct cggcatgtcc aacgacatct acttccaggt cggccttctc
     1741 acgacgatcg gtctggtgag caagaacgcc attctgatcg tggaattcgc caaggagtac
     1801 atggaacagg gcaaggatgc gatccacgcc gccgaagagg ccgtgcgtgt ccgcctgcgt
     1861 ccgattctca tgacctccct cgccttcggt ctcggcgtgc tcccgctcgc catggcccac
     1921 ggcgcgggcg ccggcgccca gaacgccatc ggcgtgggcg tgctcggcgg catgctcgca
     1981 ggcaccttcc tctgcatcgc cttcgtcccg gtcttctacg tgctcgtcga ccgcttcctc
     2041 ggcggcaaga agcgccacga aaagaaccag acccagcagg cctgatgcag gtgactgaca
     2101 tcacgacaca ttgaaatctc agggcggcgg gcctcacccg ctccgccgcc ctaacggaac
     2161 aaacatcatg tccacgacga ttttcaagat ccttcccctc tgcgcggccc tcgccctgac
     2221 ggggtgcgtc aacctcgccc cggaccacga gcgtccagcc gcgcccgtcg aacaggcgtg
     2281 gcccacgggc gaagcctacg acgcagcctc cgtgcgcagc gaagccctgc ccgaatggca
     2341 ggccttcttc acggacgagc gccttcgcaa ggtgatcgat ctcgccctca aggaaaaccg
     2401 cgacctccgc gtctcggccc tcaacattga aaaggcccgc gccctctacg gcgtgcagcg
     2461 ctccgagctc ttcccgaccg tcgccgccgc cgctagcaac gcaggcaagc acaccccggc
     2521 aagcg
//
LOCUS       DBMDTI010000290         2521 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9138_length_2521_cov_2.152066, whole genome shotgun sequence.
ACCESSION   DBMDTI010000290 DBMDTI010000000
VERSION     DBMDTI010000290.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9138_length_2521_cov_2.152066"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1923
                     /locus_tag="ACZPB6_09735"
     CDS             <1..1923
                     /locus_tag="ACZPB6_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR1184781.1"
                     /translation="PGETQMLMIGYRLPAALHPDWEPAAMATSILSDAPTGRLYKELV
                     ETGLATQVFGWTIPGAQPGFVVFGAQVKKGEDLGPVRAKMVDVIENSFARTPVTDQEL
                     ERQKAEQATMFERQISDPEAFAVGLSDYIALGDWRVFFADRDAIAQVKTAEVDKAAAR
                     YFVRDNRVVGSYIPTDELKRADITAAPSVDEYLKGFKFRQEGRTAEAFDVDQDNINAR
                     TQFVEAGGVKMALLPKKTLGETVVVQMRFLNGNLKTGANAAVSMLSTAMLSRGTSTMT
                     RDEIDDAFTQLRMEGSPFAFTTDREHLSAAVGLAGSILTDAAFPEKEFETLKRQTLVG
                     LRAKSDDPGTKARDALTEHFNTYPKGDARRTETSAELIAEVEALTLDDVRNYWRNVFG
                     TGRGYIAVVGDFDPEAVAGDLRRAVVGRKLSTVVYERPVHEFKPVASARIVIDTPEKE
                     NGTLMARVDLPANVADADAAALVTADWILGGSTGLSNRIVTRLRQKEGLSYGAGSGIT
                     LPRFGNRGHWSVGAIVAPQNVRQAEASLRDELARAVKDGITEQELAEAKRGLIEYRAV
                     NRAQDGTLAGSWINFMESGRDWSFSKDMEVAIEKLTVKDVNAAIRRIADPSKMTFVLA
                     ADLAKAREAGKDFSKQ"
     gene            2165..>2521
                     /locus_tag="ACZPB6_09740"
     CDS             2165..>2521
                     /locus_tag="ACZPB6_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1184782.1"
                     /translation="MIRAIRIRLYPTPDQKKYLAGLFGAVRFCYNKALYLKTHFYKVK
                     GVNLHPIHDLKKLIAIAKKSKKYAWLAEYVQESVRNLNKAYGRFFKKEAGYPRFKSRR
                     GEQSSYHCTGVSAGDDF"
BASE COUNT          486 a          866 c          767 g          402 t
ORIGIN      
        1 cccggcgaga cgcagatgct catgatcggc taccgcctgc ccgccgccct gcatcccgac
       61 tgggagcctg cggccatggc cacgtcgatc ctttccgacg ccccgacggg gcgcctctac
      121 aaggagctcg tcgagacggg ccttgccacg caggtcttcg gctggacgat tccgggcgcc
      181 cagcccggct tcgtggtctt cggcgctcag gtcaagaagg gcgaggacct cggtccggtc
      241 cgcgcgaaga tggtcgacgt gatcgaaaac tcctttgcca ggacgcccgt gaccgatcag
      301 gagcttgaac gccagaaggc cgagcaggcc acgatgttcg agcgccagat cagcgatccc
      361 gaagcattcg ccgtgggcct ttccgactac atcgcgctcg gcgactggcg cgtgtttttc
      421 gccgaccgcg atgcgatcgc gcaggtgaag accgccgagg tcgacaaggc cgccgcccgc
      481 tacttcgtgc gcgacaaccg cgtcgtcggc agctacatcc cgacggacga gctcaagcgc
      541 gccgacatca cggccgcgcc ctccgtcgac gagtacctca agggcttcaa gttccgtcag
      601 gaaggccgca cggccgaagc cttcgacgtc gatcaggaca acatcaacgc ccgcacgcag
      661 ttcgtcgagg cgggcggcgt gaagatggcg ctgctgccga agaagacgct cggcgagacc
      721 gtcgtcgtgc agatgaggtt cctcaacggc aacttgaaga cgggcgccaa cgccgccgtc
      781 tcgatgctct cgaccgccat gctctcgcgc ggcacgtcca cgatgacgcg cgacgagatc
      841 gacgacgcct tcacgcagct gcgcatggag ggttcgccct ttgcgttcac gaccgaccgc
      901 gagcatctct cggccgccgt gggccttgcg ggcagcatcc tcacggacgc cgccttccct
      961 gaaaaggaat tcgagaccct gaagcgccag accctcgtcg gcctgcgcgc caagtcggac
     1021 gatccgggca ctaaggcccg cgacgccctc acggagcact tcaacaccta tccgaagggc
     1081 gacgcccgcc gcacggagac gagcgccgag ctcatcgccg aggtcgaggc cctcacgctt
     1141 gacgacgtgc gcaactactg gcgcaacgtc ttcggcacgg gtcgcggcta catcgccgtc
     1201 gtcggcgact tcgatcccga agcggttgcg ggcgacctgc gccgcgccgt cgtcggccgc
     1261 aagctctcca ccgtcgtcta cgagcgtccc gtgcacgagt tcaagcccgt tgcatcggcc
     1321 cgcatcgtca tcgacacgcc tgaaaaggaa aacggcacgc tgatggcccg cgtcgacctg
     1381 ccggccaacg tggccgacgc cgatgccgcg gccctcgtca cggccgactg gatcctcggc
     1441 ggctccacgg gtctctcgaa ccgcatcgtg acccgtctgc gccagaagga gggcctctcc
     1501 tacggcgccg gcagcggcat cacgctcccg cgcttcggca accgcggcca ttggtccgtc
     1561 ggtgcgatcg tcgctccgca gaacgtcagg caggctgagg ccagcctgcg cgacgagctc
     1621 gcccgcgccg tcaaggacgg catcaccgag caggagctcg ccgaagccaa gcgcggcctg
     1681 atcgagtacc gtgcggtgaa ccgcgcccag gacggcacgc tcgcgggcag ctggatcaac
     1741 ttcatggaat ccggccgcga ctggtccttc tcgaaggaca tggaagtcgc catcgagaag
     1801 ctcacggtca aggacgtcaa cgccgcgatc cgccgcattg cggatccctc gaagatgacc
     1861 ttcgtgctcg ccgccgacct tgcgaaggcc agggaagcgg gcaaggactt ctccaagcag
     1921 tagctccctg ccgggccaga cggcatcagg cgccccgccg ctccgacgcg agcagcgggg
     1981 cgctgtcgtt tctgaacgcc cctcgcaggc ataaaatgtc ggattgttca gctaaatccg
     2041 ccaggaaccc tcgcctcggt gaggcgggga ggaatggcgg aaaagacggc gaaatgcgag
     2101 gcatacgtta aaagaggaag cctcatataa tcaaatttcc ataccgttcc ttcctctgtc
     2161 acgaatgatc cgcgccatcc gtattcgact ctatccgacg cccgaccaga agaaatatct
     2221 ggccggtctt ttcggtgccg tgcggttctg ctacaacaag gcgctgtatc tcaagacgca
     2281 tttctacaag gtgaagggcg tcaatcttca cccgattcac gacctcaaga agctcatcgc
     2341 cattgcgaag aagtcgaaga agtacgcctg gctggcggaa tacgtgcagg aatcagttcg
     2401 caatctgaac aaagcctatg gccgcttctt caagaaggaa gccggatatc cgcgcttcaa
     2461 gtcccgacgc ggcgaacagt ccagctacca ttgcaccggc gtttcggcag gcgacgactt
     2521 c
//
LOCUS       DBMDTI010000291         2504 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9202_length_2504_cov_3.023304, whole genome shotgun sequence.
ACCESSION   DBMDTI010000291 DBMDTI010000000
VERSION     DBMDTI010000291.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9202_length_2504_cov_3.023304"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="ACZPB6_09745"
     CDS             <1..1843
                     /locus_tag="ACZPB6_09745"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR1184783.1"
                     /translation="VKDTRQVFFDLFVPTPADDQVLMARINLSRLIADVANSVIGTTP
                     YIFYLEADGKPVIDRKQFRTSARSLTYSTNIPLLNDPSLRLVSLSNEHSLFTTDNLKF
                     WAIIALAGLLAVALGLLVSNQRQQHKAHQKLFAENSLRVAMSDSSVAGIRVTDREGRI
                     LFVNETFQKLVGYRTQELIGVMPPYPYWKDDISATLQDILAHPEDRQVRSIEFRVRRK
                     DGLLFDGQLNVSPLLNENRRVIGWIGELYDITEQKRARERMKAAHERFTRVVQSMNSA
                     ICVLGGDPDHPRLLFRNTPYEKLFGDSPDGAIRLVKLISEKHSLIAREGVYDAETNRW
                     FDARMQWLTWTDDTPARMIIATDITNQRETELALEAQMKRAETTQRLVTMGEMASSLA
                     HELNQPLAAISNYASGASMMLQAGKLTREDTIGALDKVNRQAQRAAAIIKRIRGFAAT
                     KKTEPQFTSLTPESVVNETMELALIQAEKLNARINTTIEPNLPAMTGDSVMLEQLLLN
                     LLKNAMEAVQPCPNHTIDLDVRLHESGSFIQFRVADHGTGIPDDAKTMLFDAFYSTKD
                     EGMGMGLNICRSIVEVHHGRIVISDTPGGGATFTFTVPVAREHPDLM"
     gene            1879..>2504
                     /locus_tag="ACZPB6_09750"
     CDS             1879..>2504
                     /locus_tag="ACZPB6_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1184784.1"
                     /translation="MFPYDYGSEAPETLGTVYVVDDDDAVRDSLKWLLEASDYRVELY
                     DSGESFIAKYDPKAIAVLVLDVRMPGMSGLEVQEHLLARKAELPIIFITGHGDVSMAV
                     NALKRGAVDFIEKPFEQAALKQLVERMLREARERHMEKERRSLNEALLAKLTPREQQV
                     LERIIHGRLNKQIADDLGISIKTVEAHRASIMDKTNSGTVADLMRVVMG"
BASE COUNT          525 a          881 c          668 g          430 t
ORIGIN      
        1 cgtcaaggat acgcgccagg tcttcttcga cctcttcgtg ccgactcctg ctgacgacca
       61 ggtcctcatg gcgcgcatca acctctcgcg cctcatcgcc gacgtagcca acagcgtgat
      121 cggcacgacg ccctacatct tctatcttga ggcggacggc aagcccgtca tcgaccgcaa
      181 gcagttccgc acatccgccc gctcgctcac atattcgacg aacattccgc tcctcaacga
      241 tccgtcgctc aggctcgtga gcctcagcaa cgagcattcg ctcttcacga cggacaacct
      301 gaagttctgg gccatcatcg cgctcgcagg ccttcttgcc gtcgcgctcg gacttctcgt
      361 gagcaaccag cgccagcagc acaaggccca ccagaagctc tttgccgaga actcgcttcg
      421 cgtggccatg agcgactcgt ccgtggcagg catccgcgtt acggaccgcg agggcagaat
      481 cctcttcgtc aacgaaacct ttcagaagct cgtcggctac cgcacgcagg agctcatcgg
      541 cgtcatgcct ccctatccgt actggaagga cgacatctcc gcaacgcttc aggacattct
      601 tgcgcacccc gaggaccgtc aggtgcgctc gatcgaattc cgcgtccgcc gcaaggacgg
      661 cctgcttttt gacggacagc tcaacgtctc tccgcttctc aacgaaaacc gccgcgtgat
      721 cggctggatc ggcgagcttt acgacatcac cgagcaaaag cgcgcccgcg aacgcatgaa
      781 ggccgcgcac gaacgcttca cgcgcgtcgt gcaatcgatg aattcggcga tctgcgtgct
      841 aggcggcgat cccgatcacc cccgccttct ctttcgcaac accccctacg aaaagctctt
      901 cggcgactcg cccgacggcg cgatccgtct cgtgaagctc atctccgaaa agcactcgct
      961 gatcgcccgc gaaggcgtct acgacgccga gaccaaccgc tggttcgacg cccgcatgca
     1021 gtggctcacc tggacggacg acacgcccgc tcgcatgatc atcgcgaccg acatcaccaa
     1081 ccagcgcgaa accgagcttg cgctcgaggc ccagatgaag cgcgccgaaa cgacacagcg
     1141 actcgtcacg atgggcgaga tggccagctc cctcgcacac gagctcaacc agcctctcgc
     1201 cgccatctcg aactacgcct ccggcgccag catgatgttg caggcaggca agctcacgcg
     1261 cgaggacacg atcggagcgc tcgacaaggt gaaccgtcag gcccagcgcg cagcggccat
     1321 catcaagcgc atccgcggct tcgccgccac caagaagacc gagccgcagt tcacgtcgct
     1381 cacgcccgaa agcgtcgtca atgagacgat ggagcttgca ctcattcagg cggagaagct
     1441 caacgcacgc atcaacacga ccatcgagcc gaaccttccc gccatgaccg gcgactccgt
     1501 catgcttgag caattgcttc tcaacctgct caagaacgcc atggaggccg tgcagccctg
     1561 tcccaaccac acgatcgatc tggacgtgag gctccacgag agcggcagct tcatccagtt
     1621 cagggtggcc gaccacggca cgggcattcc agacgacgcc aagacgatgc tcttcgacgc
     1681 cttctactcg acgaaggatg agggcatggg catgggcctc aacatctgcc gctcaatcgt
     1741 cgaagtccac cacggccgaa tcgtcatctc cgacacgccg ggtggcggcg ccacctttac
     1801 attcaccgta cccgtggctc gtgaacatcc tgatctgatg tagaatcagg gtactttttc
     1861 tgaggagaga tttctttcat gtttccctat gactacggtt ccgaagcccc tgaaacactg
     1921 ggcacggtct atgtcgtcga cgatgacgac gccgtccgcg actccctgaa gtggctcctc
     1981 gaggcgagcg actaccgcgt cgagctctac gacagcggcg agagcttcat cgccaagtac
     2041 gacccgaagg ccattgcggt tctcgtgctt gacgtgcgca tgccgggcat gagtggtctt
     2101 gaagtgcagg aacaccttct ggcccgcaag gccgagctgc ccatcatctt catcacgggc
     2161 cacggagacg tgtccatggc cgtgaacgcc ctcaagcgcg gcgccgtcga cttcatcgaa
     2221 aaacccttcg agcaggccgc cctcaagcag ctcgtcgagc gcatgctccg cgaagcccgt
     2281 gaacgccaca tggagaagga acgccgcagt ctcaatgaag ccctgctcgc caagctcacg
     2341 ccgcgcgagc agcaggttct cgagcgcatc atccacggcc gtctcaacaa gcagatcgcc
     2401 gacgacctcg gcatctccat caagaccgtc gaagcccacc gagcctccat catggacaag
     2461 accaactcgg gcacggtcgc cgacctcatg cgcgtcgtca tggg
//
LOCUS       DBMDTI010000292         2498 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9231_length_2498_cov_1.759282, whole genome shotgun sequence.
ACCESSION   DBMDTI010000292 DBMDTI010000000
VERSION     DBMDTI010000292.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9231_length_2498_cov_1.759282"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="ACZPB6_09755"
     CDS             <1..195
                     /locus_tag="ACZPB6_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR1184785.1"
                     /translation="APTKAEAVAVAKRIHSGNVYINDAPRDVTAPFGGYKQSGIGREG
                     GLAGLLEFTQQKAVFDRTTY"
     gene            complement(275..1459)
                     /locus_tag="ACZPB6_09760"
     CDS             complement(275..1459)
                     /locus_tag="ACZPB6_09760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1184786.1"
                     /translation="MHVTVYLICALVTALMAVYGIWRTIRIAHLGTTSKLAVTAFFAL
                     AGASHFAFLDMTDLFERAFIVDTLPVTGLFVSFAYWFFLLALARDVIGGVRGLVRRKM
                     GLPVEKRDLFTIVSTCFVAVFGIFFGITSTWNALSEPTVEPMTVAVRNLPPELEGFKV
                     AQITDLHASNLFPAARTEAVVKAVNEARPDLVLITGDFSDGPLDREAAVLQRDADLAP
                     LSGLKAAYGIFGVTGNHEYIRPEREKLLEIIRRHGVVILENEAVSIAVKGRRVTVAGV
                     DDLAAERTGAGRHDLAGTLSNLADDDLRILMDHQPRTAPEAANPAWKVDLQLSGHTHG
                     GHTGPVAILASLANNGFVKGFYEVGGMKLYVSQGAGFWAGFPVRSGTFNEIPLITFTR
                     AN"
     gene            complement(1690..>2498)
                     /locus_tag="ACZPB6_09765"
     CDS             complement(1690..>2498)
                     /locus_tag="ACZPB6_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1184787.1"
                     /translation="RGRLLLSAEGRRLKPAADELMERTDDIESLFSGRTTGQLRLACT
                     FTIGSFMLSDLMRDFQAHAGWMPEMRIANTAEVAESLLNFSTDISIVEGPVTNPHLIT
                     EPWISDEMVVVAPKGHRLTQGVATWEQLSRERWILREEGSSTRIFFDAQIGLHLSEHN
                     IIANVNSFEAIVGMIINGMGLTFISERILHDPFFGPHLRRVKCPETFTRQLSFCMHKQ
                     KYRSSDLQAFLAFCRLWGEQLLEREHSRRLEEEQAAMVFTSPSRFSGEVA"
BASE COUNT          490 a          710 c          887 g          411 t
ORIGIN      
        1 gccccgacga aggccgaggc ggtcgcggtc gcaaagcgca tccactcggg caacgtctac
       61 atcaacgacg ccccgcgcga cgtcaccgcg cccttcggcg gctacaagca gagcggcatc
      121 ggacgtgagg gcggcctcgc gggcctgctc gaattcacgc agcagaaggc cgtcttcgac
      181 cgaacgacat actgacacct gcccaaactc atcaaaaaag cctcccgaag tcgggaggct
      241 ttttcatgcc gggaatgctc gaaaagctgc tcgatcagtt ggcgcgcgtg aacgtgatga
      301 gcgggatttc gttgaaggtg ccgcttctca cggggaagcc cgcccagaag cccgcgccct
      361 gggagacgta gagcttcatg ccgcccacct cataaaagcc cttgacgaag ccgttgttgg
      421 caagcgaggc gagaatcgcg acgggacccg tgtggccgcc gtgggtgtga cccgaaagct
      481 gcagatccac cttccaggca gggttcgcag cctcgggagc cgtgcggggc tgatggtcca
      541 tcaggatgcg gaggtcgtcg tcggcaagat tggagagcgt acccgcaagg tcgtgacggc
      601 ctgcgcccgt gcgctcggcg gcgaggtcgt ccacgcccgc caccgtcacg cggcggccct
      661 tgacggcgat ggagacggcc tcgttctcga ggatcacgac gccgtggcgc ctgatgatct
      721 caaggagctt ctcgcgctcg ggacggatgt actcatggtt gcccgtcacg ccgaagatgc
      781 cgtaggcggc cttgaggccg gaaagaggcg cgaggtccgc gtcgcgctga agaacggcag
      841 cctccctgtc gagcggaccg tcggagaagt cgcccgtgat gaggacgagg tcgggcctgg
      901 cctcattgac ggccttcacc acggcttcgg tgcgggcggc ggggaagagg ttcgaggcgt
      961 gcaggtccgt gatctgggcg accttgaagc cctcgagctc gggcggaagg ttcctgacgg
     1021 ccacggtcat cggctcgacc gtaggctcgg agagcgcgtt ccaggtcgag gtgatgccga
     1081 agaagatgcc gaagacggcc acaaagcacg tcgagacgat cgtgaagagg tcgcgcttct
     1141 cgacgggaag ccccatcttc ctgcgcacga gtccgcgcac gccgccgatc acgtcgcgcg
     1201 cgagcgccag caggaagaac cagtaggcaa agctcacgaa gagccccgtg acgggcagcg
     1261 tgtcgacgat gaaggcgcgc tcgaagaggt ccgtcatgtc gagaaaggcg aagtgcgagg
     1321 cgcccgcaag cgcaaagaag gccgtgacgg caagcttcga ggtcgtgccg agatgggcga
     1381 tgcggatcgt gcgccagatg ccgtagacgg ccatcagtgc cgtgacgagc gcgcagatga
     1441 gatagacggt gacgtgcatg gaaatggaat cggtcttgtg gtctggtggt ttgggagact
     1501 tctgcggaaa gcctgaggga ggcccgtccg aacgtgaagc ggcaggcatt gtacacggag
     1561 gcgcaggaat actggtgctg caaggcgtcg aattcttgcg caatgtcctc atctgcggac
     1621 ggctttgcca ttcccaagag aatggctgag gaaggaagac cggatgcagc agggctgcag
     1681 gtcaggaaac taggcaacct cgccgctgaa tcgggaggga cttgtgaaga ccatggccgc
     1741 ctgttcctcc tcgagcctgc ggctgtgctc gcgttcgaga agttgttcgc cccaaaggcg
     1801 gcagaaggcg aggaaagcct ggaggtcgga ggagcggtac ttctgcttgt gcatgcagaa
     1861 ggagagctgc cttgtgaagg tctcgggaca cttgacgcgc ctgaggtgcg ggccgaagaa
     1921 cggatcgtgg aggatgcgct cggagatgaa ggtgaggccc atgccgttga tgatcatgcc
     1981 gacgatcgcc tcgaagctgt tgacgttcgc gatgatgttg tgctcggaga ggtgaaggcc
     2041 gatctgggcg tcgaagaaga tgcgggtgct cgagccctcc tctcgaagaa tccagcgctc
     2101 gcgagagagc tgctcccagg tcgcgacgcc ctgtgtgaga cggtggccct tgggcgccac
     2161 cacgaccatt tcgtcggaaa tccacggctc ggtgatgagg tgcggatttg tgacggggcc
     2221 ctcgacgatc gagatgtcgg tcgagaagtt gaggagcgat tcggccacct cggcggtgtt
     2281 ggcgatgcgc atttcgggca tccagcccgc gtgcgcctga aagtcgcgca tcaggtccga
     2341 gagcatgaag gagccgatcg tgaacgtaca ggcaagcctg agctggcccg tcgtgcgacc
     2401 cgaaaagagc gactcgatgt cgtcggtgcg ctccatgagc tcgtcggcgg cgggcttgag
     2461 gcgtctgcct tcggccgaaa gaaggagtcg gccgcggg
//
LOCUS       DBMDTI010000293         2497 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9239_length_2497_cov_1.969533, whole genome shotgun sequence.
ACCESSION   DBMDTI010000293 DBMDTI010000000
VERSION     DBMDTI010000293.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9239_length_2497_cov_1.969533"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            59..913
                     /gene="menB"
                     /locus_tag="ACZPB6_09770"
     CDS             59..913
                     /gene="menB"
                     /locus_tag="ACZPB6_09770"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HIR1184788.1"
                     /translation="MQPMLDTSHLPPVPEWHKAGEFTDIVYEKCSDGIAKITINRPQV
                     HNAFRPQTVMEMSRVLNDARNDADVGVIILTGMGENAFCSGGDQKVRGTGGYVGDDGT
                     PRLNVLDLQREIRTCPKPVVAMVAGWCVGGGHVLHMMCDLTIAAENAMFGQTGPRVGS
                     FDGGWGASYMARIVGQKKAREIWFLCRFYNAKEALDMGLVNTVVPLEKLEAETVQWCR
                     EMLANSPLALRCLKAALNADCDGQAGLQELAGNATLLYYMTEEGREGKNAFLEKRRPD
                     FSKFQRLP"
     gene            921..2285
                     /locus_tag="ACZPB6_09775"
     CDS             921..2285
                     /locus_tag="ACZPB6_09775"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR1184789.1"
                     /translation="MPSPTTGAGPSTSVAALALSAPDRTAVVDVSRRITWAELARLVS
                     DRTASLLQPASGRPAFLIPKPDLNGLVDILAHWEAHIPAVLVSPKLTSAEQDALKSHA
                     DSIAEPLPDGTAFVVFTSGTTGLPKPAILTRESLTAGARAMILALRMTSSDVLQLSLS
                     PARVGGLGIIMRTLAAGSTIALSPGFSGARFPETLGRDGVTFASLVPAMLADVLERNP
                     DWRNPPHLRAILIGGAPFPDRLRAEAARRGIPIMTTYGMTETGSTSAVSAYETRLSPV
                     PVGEVPLAGTSFRTVDGVLCLKGPMLFAGYWGRPAPVHDGWFETGDAGLVNHDGSIRI
                     LGRTSDMIITGGEKVVPSEVESALESLPGIREALVVGMPDEKWGQIVTALLVSDTDPH
                     PQSDAIVCGLAARLARWKSPRRIAWVSSLPFTSAGKRSRNPEVLRDLEFDVLHYRHST
                     EAPL"
BASE COUNT          421 a          937 c          684 g          455 t
ORIGIN      
        1 cccgcgcaca gcgctaaagg ccgaacccat acagactgtt tgaaaaggaa ttcacgacat
       61 gcagcccatg ctcgacacct cacatcttcc tcccgttccc gaatggcaca aggcgggcga
      121 attcaccgac atcgtctatg agaagtgctc ggacggcatc gccaagatca cgatcaaccg
      181 tccccaggtt cacaacgcct tccgtccgca gacggtgatg gagatgagcc gcgtgctcaa
      241 cgacgcccgc aacgacgcag acgtcggcgt catcatcctg accggcatgg gcgaaaatgc
      301 cttctgctca ggcggcgatc agaaggtccg cggcacgggc gggtacgtcg gcgacgacgg
      361 cacgccccgc ctcaacgtcc tcgacctgca gcgcgagatc cgcacctgcc cgaagcccgt
      421 cgtcgccatg gtggcgggct ggtgcgtggg cggcggtcac gtccttcaca tgatgtgcga
      481 cctcacgatt gccgccgaga acgccatgtt cggtcagacg gggccgcgcg tcggttcctt
      541 cgacggcggc tggggcgcat cctacatggc acgcatcgtc ggccagaaga aggcacgcga
      601 gatctggttc ctctgccgct tctacaacgc caaggaggcc cttgacatgg gtctcgtcaa
      661 cacggtcgtt ccgcttgaga agcttgaagc cgaaaccgtc cagtggtgcc gcgagatgct
      721 tgccaactcc ccgctcgccc tccgctgcct caaggccgcc ctcaacgccg actgcgacgg
      781 ccaggcgggc ctgcaggagc tcgcgggcaa tgccacgctc ctctactaca tgaccgaaga
      841 gggccgcgaa ggcaagaacg cctttcttga aaaacgccgt cccgacttct cgaagttcca
      901 gcgcctgccc tgaggctgac atgccctctc ccaccacggg cgccggcccc tccacgagcg
      961 tcgccgccct cgcactctcc gcgcccgacc gcacggccgt tgtcgacgtc tcccgtcgca
     1021 tcacgtgggc ggaactcgcc cgtctcgtct cggatcgtac ggcctcgctt ttgcagcccg
     1081 cttccggccg tccggccttc ctcatcccaa agcccgacct gaacggcctc gtcgacatcc
     1141 ttgcccattg ggaggcgcac attcccgcgg tcctcgtctc gcccaaactc acttctgccg
     1201 agcaagacgc cctcaaatcc cacgcggact ccatcgcaga acctcttccc gacggcaccg
     1261 cattcgtggt ctttacgtct ggaacgacgg gccttccgaa gcccgccatc ctcacgcgcg
     1321 agtccctgac ggcaggcgcc agagcgatga tcctcgccct tcgcatgacc tcgtccgacg
     1381 tcttgcagct ctcgctctcg cccgcccgcg tgggcggcct cgggatcatc atgagaaccc
     1441 tggccgcagg cagcacgatc gcgctgtctc cgggcttttc gggcgcccgc tttcccgaga
     1501 ccctcggccg agacggcgtg acgttcgcct cgctcgtccc cgccatgctg gccgacgtcc
     1561 tcgagcgcaa tccggactgg cgcaatccgc cccacttgcg cgccatcctc atcggcggcg
     1621 ccccgttccc cgaccgtctt cgcgccgaag ccgcccgacg cggcattccc atcatgacaa
     1681 cctacggcat gaccgaaacg ggcagcacct ccgcggtctc ggcctacgaa acccgcctct
     1741 cgcctgtccc tgtcggcgaa gtgccgctcg cgggcacctc gttcaggacc gtcgacggcg
     1801 tcctctgcct caagggaccg atgctctttg ccggctactg gggacgcccc gcacccgttc
     1861 atgacggctg gttcgagacg ggcgacgcgg gactcgtcaa tcatgacggt tcgatccgca
     1921 tcctcgggcg cacctccgac atgatcatca cgggcggtga aaaggtcgtt ccctccgaag
     1981 tcgagtcggc ccttgaatcg cttcccggga ttcgcgaggc gctcgtcgtc ggcatgcccg
     2041 atgaaaagtg ggggcagatc gtcacggctc ttctcgtctc cgacaccgat ccccatccgc
     2101 agtctgacgc catcgtctgc ggacttgccg cacgcctcgc ccgatggaag tctcctcgac
     2161 gcatcgcatg ggtcagctcc ctgccgttca cctccgcggg caagcgaagc cgcaaccccg
     2221 aggttcttcg ggacctagaa ttcgatgttc ttcactacag acactcaacc gaagccccgt
     2281 tgtaatccgc ctcccggatt tcgtctcctc ccctttttgt cttttccaaa ggaccgctgc
     2341 ggacatctgc accgccgcgg cccgtttttc atgaccgccg gccactacgc actcacaatt
     2401 gcattcgagc cgccgcaccc ctctttctga acggggacac gatgtccttc agaacgttca
     2461 ggcgcagtcg tccagtgtct ccacactttc ccgttcg
//
LOCUS       DBMDTI010000294         2486 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9287_length_2486_cov_2.270440, whole genome shotgun sequence.
ACCESSION   DBMDTI010000294 DBMDTI010000000
VERSION     DBMDTI010000294.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9287_length_2486_cov_2.270440"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="ACZPB6_09780"
     CDS             complement(<1..1027)
                     /locus_tag="ACZPB6_09780"
                     /EC_number="5.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase family
                     protein"
                     /protein_id="HIR1184790.1"
                     /translation="MAMKTYDFAYGRGRKTFTVEEDRVLKVVRTEEFPPMEDIRGGVL
                     EAIRHPIGCPSIEEIVKPGDTVAFICNDPTRVANSFDFMPVLVDEMNRLGVKDEDMKI
                     VFALGTHRAMTHEEMVEGVGENVASRLKMYNSICTNNDDFEYFGTTSRGTPVHIHKEL
                     CNVDHVILTGTIVHHYFSGYGGGRKAILPGCAAMETVRVNHSFMLDENAALGRTTGNP
                     CYEDQMEGVALFAKGRSLFLFNAILNAKHQFLRMFAGDYIAAHKEACKFVDEVYGCVI
                     PKLADMVIVSCGGYPKDINVYQMQKTMENASCAVRKDGVVILLADCEEGSGSKVLEET
                     FRRLKTPA"
     gene            1359..1811
                     /locus_tag="ACZPB6_09785"
     CDS             1359..1811
                     /locus_tag="ACZPB6_09785"
                     /inference="COORDINATES: protein motif:HMM:NF023742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1184791.1"
                     /translation="MKANIAYKFELMPTREQTRIFAQAAGCRRFVWNKATELQKQRLE
                     HNLPFLNYAELCEKLTCWKQEDDMLWLQDAPSQALQQGLKDECASINEFKSNKKGFPK
                     FHPSRSTRNELPFFKSNFATTRKPARSWPIWARRPRLTNGSRVAGAGN"
     gene            1694..2317
                     /locus_tag="ACZPB6_09790"
     CDS             1694..2317
                     /locus_tag="ACZPB6_09790"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HIR1184792.1"
                     /translation="MAVLQKQLCHNKEARKKLADLGKAAPFDKREPSRRCRQLKNQIL
                     THYRRIRNIRQDFMKRTAHTIAQKYGWVAMENLKLKNMTKSAKGTVEDPGRNVKQKSG
                     LSRSLARVAPYGMRMAILWALFKAGGRLILADPKYTSQTCPKCGYTSSSNRPTQAHFG
                     CQLCGYTENADVVGALNVLKKSRTGSVRPSSELGNKSAAGTVLFFAS"
BASE COUNT          534 a          669 c          768 g          515 t
ORIGIN      
        1 cggccggagt cttgaggcgg cggaaagtct cctcgagcac cttggagccc gagccttcct
       61 cgcagtcggc gagcaggatc acgacgccgt ccttcctgac ggcgcaggag gcgttctcca
      121 tggtcttctg catctggtag acgttgatgt ccttgggata gccgccgcag gagacgatca
      181 ccatgtcggc gagcttcgga atgacgcagc cgtagacctc gtcgacgaac ttgcaggctt
      241 ccttgtgcgc ggcgatgtag tcgcccgcga acatgcgcag gaactggtgc ttggcgttca
      301 ggatggcgtt gaagaggaag agcgagcggc ccttggcgaa gagcgcgacg ccctccatct
      361 ggtcctcgta gcaggggttg cccgtcgtgc ggccgagcgc ggcgttttcg tcgagcatga
      421 agctgtggtt gacgcgaacg gtctccatcg cggcgcagcc gggaaggatg gcctttcggc
      481 cgccgccgta gccggagaag tagtggtgca cgatcgtgcc cgtgaggatc acgtggtcga
      541 cgttgcagag ctccttgtgg atgtgcacgg gcgttccgcg ggaggtcgtg ccgaagtatt
      601 cgaagtcgtc gttattcgtg cagatgctgt tgtacatctt gaggcgggag gcgacgtttt
      661 cgcccacgcc ctcgaccatt tcctcgtgcg tcatggcgcg gtgggtcccg agcgcgaaga
      721 cgatcttcat gtcctcgtcc ttcacgccga gacggttcat ctcatcgacg agcaccggca
      781 tgaagtcgaa gctgttggcg acgcgggtcg ggtcgttgca gatgaaggcg acggtgtcgc
      841 cgggcttgac gatctcttcg atcgacgggc agccgatcgg gtggcggatc gcctcgagca
      901 cgccgcccct gatgtcctcc atcggcggga actcttcggt gcggaccacc ttcagaacgc
      961 ggtcctcttc aacggtaaag gttttgcgac cgcgcccgta ggcgaagtcg taggtcttca
     1021 tggccatgtg ttgtctcctt gattcggcat gcgaggggcg gactccgtcc cgtcttcaaa
     1081 atggtcttct cgggatgcgg aggtcatgcg aaagcagtag tgctttcgag ggtccggctc
     1141 tgccgtagat gcattatagg tgccgcgcaa tccttttgag aaagggcttt caaggcaaag
     1201 cccagtcagg gcgcggaagg tcaagccttc gggtcaaact tacaaatcca agccgagaaa
     1261 gccctcgatt tcaatcgatg ggatgagagg tactcttctg gttcgcagat tgtctacgaa
     1321 ccatgcgttc tcgaaaagga cggtgtagga tggaagtcat gaaagccaat atcgcctaca
     1381 agttcgaact gatgcctacc cgggagcaaa cgcgaatctt cgctcaagcc gccgggtgcc
     1441 ggcgttttgt ttggaacaag gcgaccgaat tgcagaagca gaggcttgaa cacaatctgc
     1501 cgtttctgaa ctatgcggaa ctttgtgaaa agctcacgtg ctggaaacag gaagacgaca
     1561 tgttgtggct tcaggatgcg ccatctcaag cgcttcagca aggtctgaag gatgagtgcg
     1621 cctccatcaa tgagttcaag tccaataaga agggcttccc gaagtttcat ccatcaagaa
     1681 gcacgagaaa cgaattgccg ttcttcaaaa gcaactttgc cacaacaagg aagcccgcaa
     1741 gaagctggcc gatttgggca aggcggcccc gtttgacaaa cgggagccga gtcgcaggtg
     1801 caggcaactg aagaaccaga ttctgacgca ctatcgtcgt atccgcaaca ttcgtcagga
     1861 cttcatgaag agaacggctc acaccatcgc acagaaatac ggatgggtgg ccatggaaaa
     1921 tctgaagttg aagaacatga cgaaatcggc caagggcacg gttgaggatc cgggcaggaa
     1981 cgtgaagcag aagagcggct tgagccgctc tttggcacgc gttgcgccct atggtatgag
     2041 gatggccatc ctgtgggcct tgttcaaggc cggcggacgg ctcatccttg ccgatccgaa
     2101 gtacacgagc cagacttgtc ccaagtgcgg atacacgagt tcatcaaatc gacctacgca
     2161 ggcgcatttc ggttgccaac tttgcggcta cactgaaaac gcggacgtcg taggtgcact
     2221 caacgtgcta aagaagagcc ggacaggctc agttcgcccg tcaagtgaac ttggcaacaa
     2281 gtcagcagcg ggaaccgtcc ttttcttcgc gtcctgacgc gaatacgagg aatcccgggt
     2341 attcatactc gggaggacgt caaacttccg aggtagggga tgccccgaat ggccggtcgg
     2401 ggcactctct caaatggaat tgccttcagc aatcacttcc cgtcgacgct gttgacgaga
     2461 ttcatgcgca tgatggcgcg gtgctt
//
LOCUS       DBMDTI010000295         2473 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9348_length_2473_cov_3.395868, whole genome shotgun sequence.
ACCESSION   DBMDTI010000295 DBMDTI010000000
VERSION     DBMDTI010000295.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9348_length_2473_cov_3.395868"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="ACZPB6_09795"
     CDS             complement(<1..245)
                     /locus_tag="ACZPB6_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1184793.1"
                     /translation="MLALVLGAFVAAGPSVVVTPVQAATTQKAVKAGVKKTSVRKTVK
                     KTTSVKRTNSRKTLGRAKHAVKKSSKKPRRQARRRVTA"
     gene            complement(460..>2473)
                     /locus_tag="ACZPB6_09800"
     CDS             complement(460..>2473)
                     /locus_tag="ACZPB6_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HIR1184794.1"
                     /translation="PPYLGARGIKFWIWPGSARAKKAGRWILAAEIVETSRMFARCVA
                     DIEPEWIEAAAGDLLRRNWTEPHWEKSRGEVVAFERGTLYGLTIYQQRRVSFAPHDPK
                     LARELFIRQALVEGDWDGRAEFYAHNARLVREIQDLEHKTRRPDVLVDDELMFAFYDE
                     RIPADVVSTPTLLKWLKATSRDDPKALFMSRDELMRHDASGVTNRYFPKTMEMAGISM
                     ALNYHFEPGSPRDGLTLAVPLYALNQLDAVRAEWLVPGMVKEKAQTLLKSLPQKIRRH
                     CVPIADFAGGFFTRTKEGEPQAKGFLEALADDVRSQTGVPCTVGDFKLEQLPPHLIMN
                     FKVLDEHGRQIGMGRNLAQLRAEFGEAAQETFRHVAQEDAAVAKDLEDGITDWTFGEL
                     PELMEISRRGQTLIGHPALTDRGTDCAIEVYDDPLEARREHRKGLLRLFRLALREQVK
                     FVERTLRDLGRVQMQAQTVPGLSRLFESADTLSTSVVDCALEATALAEPWPHDEASFR
                     ERREDVKGRLTLVAGEISRLLTEIVTEAAGIPLKLRRLSGEKALTDDIEAQLNDLFRK
                     DFLTTVPLSQLAHYPRYLRAVHYRLDRYRDDPVRDESRRADIERLSVPLMRARAARRG
                     QPDSRLDEFGWMLQELRVSLFAQQLRTPMPVSVKRLERVWMSITRL"
BASE COUNT          484 a          796 c          711 g          482 t
ORIGIN      
        1 gccgtcacgc gcctgcgcgc ctgtctgcgc ggcttcttgc tcgacttctt gaccgcatgt
       61 ttggccctgc ccagcgtctt gcggctgttc gtacgcttga cggaggtggt cttcttgacg
      121 gtctttctga ccgacgtctt ctttacaccc gccttcactg ccttctgagt cgtcgccgcc
      181 tgcacgggcg tcacaacaac cgacgggcct gccgccacga atgcgcctag cacaagtgcc
      241 aacacgattt tcttcaagct gaatttcacg accaccgccc acgacctaca gaaggtccgt
      301 caaatcccaa acaagccgct gcgccggatg cgccggcgca gtacattttg ttaattttaa
      361 aagttactgt cggattgcgg aaccgacggc atcaagaaac ccgacaccgt acgcgagaga
      421 aaatcgttag cacttgtttc tgcaagacgt cgtcaagtgt catagtctcg tgatcgacat
      481 ccacacgcgc tcgagacgct tgacactcac tggcatcggc gttcgcagct gctgggcgaa
      541 aagcgagacg cggagctcct gcaacatcca tccgaattca tcaagacgcg agtcgggctg
      601 cccgcgacgc gccgcacgag ccctcatgag cggtacggaa agcctctcga tgtccgcacg
      661 gcgggattcg tcgcgtacgg gatcgtcacg atagcggtca agcctgtagt gcaccgccct
      721 cagatagcgg ggatagtgcg cgagctgaga aagcggcacc gtcgtcagaa aatcctttct
      781 gaacagatcg ttgagctgag cctcaatgtc atccgtcaga gccttctcgc cgctcagacg
      841 gcgaagcttc aggggaatgc ctgcggcctc cgtcacaatc tcggtgagaa gacgcgagat
      901 ttcaccggcc accagcgtga ggcgcccctt cacgtcctcg cgtcgctccc tgaagcttgc
      961 ctcatcgtga ggccagggtt cggcaagcgc cgttgcctcg agcgcgcagt cgacgacgga
     1021 agtcgacagc gtgtccgcgc tctcgaagag tctggacagt cctggcaccg tctgagcctg
     1081 catctgcacg cggccaagat ctcgaagcgt tctttcaacg aacttcacct gctcgcgaag
     1141 agcgagcctg aagagacgca gaagcccctt tctgtgctct cgacgggcct ccagcggatc
     1201 atcgtagacc tcgattgcac agtcggttcc gcgatccgta agcgcaggat gaccgatcag
     1261 ggtctgcccc ctgcgggaaa tctccatgag ctcaggaagc tcgccgaagg tccagtcggt
     1321 gatgccgtcc tcaagatcct ttgctacagc cgcatcctcc tgagcgacgt ggcgaaaagt
     1381 ctcctgtgcc gcctcgccga actcggcgcg caactgtgca agattgcgcc ccatgccgat
     1441 ctggcgacca tgctcgtcca acaccttgaa gttcatgatg agatgcggcg gcagctgctc
     1501 gagcttgaag tctccgacag tgcagggaac gccagtctgg gaccgcacgt cgtcggcaag
     1561 cgcttcaagg aagcccttcg cctgcggctc tccctccttc gttcgcgtga agaagccgcc
     1621 cgcaaaatcg gcaatcggca cgcagtggcg cctgatcttc tgcgggagcg acttgagcag
     1681 cgtctgagcc ttctccttca ccatgcccgg cacgagccat tcggcgcgta cggcgtcgag
     1741 ctggttgagg gcgtagagcg gcaccgcaag cgtcagaccg tcccggggac tgccgggctc
     1801 gaagtggtag ttgagtgcca tcgaaatacc cgccatctcc atcgtcttcg ggaaatagcg
     1861 gttggtgacg cctgatgcgt catgacgcat cagctcgtca cgggacatga agagcgcctt
     1921 cggatcgtcg cgggaggttg ccttgagcca cttcaacagc gtcggcgtgg agacgacatc
     1981 tgccggaatc ctctcatcgt agaaggcgaa catcagctcg tcgtccacaa gcacgtcggg
     2041 gcggcgagtc ttgtgctcga gatcctgaat ctccctgacc agcctggcat tgtgtgcata
     2101 gaactccgca cggccatccc aatcgccctc aacgagcgct tgacggatga agagttcgcg
     2161 tgcgagcttc ggatcgtgcg gcgcaaagct caccctacgc tgctggtaga tcgtgagacc
     2221 gtagagcgtg ccgcgctcga atgcaacaac ctcccctcgg ctcttctccc aatggggctc
     2281 ggtccaattc ctgcgaagca gatcgcctgc agccgcctcg atccattcgg gctcgatgtc
     2341 cgccacgcag cgcgcgaaca tgcgggacgt ttcgacgatt tcggcagcca gaatccagcg
     2401 acccgccttc ttggcgcgcg ccgatccagg ccagatccag aacttgatgc cgcgagcgcc
     2461 aaggtaggga ggc
//
LOCUS       DBMDTI010000296         2471 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9355_length_2471_cov_1.982278, whole genome shotgun sequence.
ACCESSION   DBMDTI010000296 DBMDTI010000000
VERSION     DBMDTI010000296.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9355_length_2471_cov_1.982278"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1606)
                     /locus_tag="ACZPB6_09805"
     CDS             complement(<1..1606)
                     /locus_tag="ACZPB6_09805"
                     /inference="COORDINATES: protein motif:HMM:NF021352.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2357 domain-containing protein"
                     /protein_id="HIR1184795.1"
                     /translation="MTAEDAGAGVQAGTLVLTVRPEGAAPVPLPLHEAEPLIGDWSGQ
                     PAVEAGVPMSLEVSGLPPRAAVTVRMGGEVLAGDQYREEDCVHLELQDPLPFDNMIGV
                     AKLEVLVSMGPQACSFFYAEPLRIILNPGLASDNLLRMSESVAEHVERLFGGDAVRTG
                     DDSLGDLLGVLERLSSLYERQYPYFREDPKCRLASTARRMSIERMRDMTPEAARWMAS
                     HPHEWQRVAPGHGIRMRGANWMPAHALVRTSELSRDLYENRVVAGFPLHLASETRRLA
                     QAFEDAVRVGAGPLDAGTIGRMAAVRIVSSEADVLRGYGIVFSRIAELYRDALGVTPE
                     PVNRLPAPARHFIETSQYRLVYELMREWFELRPVEAEKLVSRLASVTSPRLYEYFSLV
                     SLLGGLERRGWTSVEAKRHEYAGAGPLYADNAAAALLNTFIFRRKEDDGGNEEIVRLW
                     YQPVLAAGNVEPENGIGLARTTRWSVRPLNAEAADTLRETDRAWYSPDFLISLESGGR
                     TAWAVMDSKYSTVGTVLRHHAMQQAFK"
     gene            complement(1618..>2471)
                     /locus_tag="ACZPB6_09810"
     CDS             complement(1618..>2471)
                     /locus_tag="ACZPB6_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184796.1"
                     /translation="AGLSAGRFVHVPVEYGWTSKRDFVGYWNPITNRFESSDPARWDL
                     LCTLDAEERSASGSVYPAMMLLDEANLSPMEYYWSDWMRLCDEQTSSGIVTLWDKAPT
                     KVPETLRFMATINNDSTTETLSSRLIDRAAVVTLPVVDCIENTSPAKVVGPVSWKELQ
                     AYFGAKAISKNARELSGLHDRLMPMLEGFGIRLSPRSIRQMNGYVGAASSIFADGDKP
                     AWLDAADFAVMQKCLPRITGTGAAYREKLVEFRSGLESLGLSRSVEVVDRILRQGDEA
                     MDCYRFF"
BASE COUNT          442 a          875 c          741 g          413 t
ORIGIN      
        1 acttgaaggc ctgttgcatc gcatggtgcc gcagcaccgt tcccacggtc gagtacttgc
       61 tgtccatgac cgcccaggcc gtcctgcctc cgctctcgag cgaaatcagg aagtcgggcg
      121 aataccaggc cctgtccgtc tccctcagcg tgtcggcagc ctccgcattg aggggcctca
      181 cggaccagcg tgtcgtcctc gcaagtccga tgccgttctc gggctcaaca tttcccgccg
      241 ccagcacggg ctggtaccag agcctcacga tctcctcgtt ccctccgtcg tcttccttcc
      301 tcctgaagat gaaggtgttg agaagggccg cggccgcatt gtccgcataa agcggacccg
      361 ctcccgcata ctcgtgcctc ttcgcctcca cgctcgtcca gcccctgcgc tcaaggccgc
      421 cgagaagtga aacgagcgaa aagtactcgt agagcctcgg actcgtcacg ctcgcaagcc
      481 tgctcacgag cttctcagcc tcgacgggcc tgagctcgaa ccactcgcgc atcagctcgt
      541 agacgagcct gtactgggac gtctcgatga aatgcctcgc gggcgcgggc agcctgttca
      601 cgggctcggg cgtcacgccg agcgcgtccc ggtagagctc cgcaatgcgc gagaacacga
      661 tgccgtagcc cctcaggacg tcagcctccg aactcacgat gcgcacggcg gccatgcggc
      721 cgatggtccc cgcgtcaaga ggtcccgcgc cgacgcgcac cgcgtcctcg aacgcctgcg
      781 ccagacgcct cgtctccgac gcaagatgca gcggaaagcc tgccaccacg cggttttcat
      841 aaaggtccct gctcagctcg gacgtgcgca cgagcgcatg agcgggcatc cagttcgcgc
      901 cccgcatgcg gatgccgtgt cccggcgcca ctctctgcca ctcgtgcgga tgcgaggcca
      961 tccagcgcgc ggcctcgggc gtcatgtcgc gcatgcgctc gatgctcatg cgtctggcag
     1021 tggaggcgag cctgcacttg ggatcctcgc ggaaatacgg atactgcctc tcgtagagag
     1081 acgacagacg ctcgagcacg cccagaagat cgccaagcga atcgtcgccc gtcctgacgg
     1141 catcgccccc gaagagcctc tccacgtgct ccgccacgct ctcgctcatc ctgagcagat
     1201 tgtccgaggc aagccccgga ttgagaatga tgcgaagcgg ctcggcatag aagaagctgc
     1261 acgcctgagg tcccatgctc acgagcacct cgagcttcgc cacgccgatc atgttgtcga
     1321 agggaagcgg atcctgcagc tcgaggtgta cgcagtcctc ctcgcgatac tggtcgcccg
     1381 cgagcacctc gccgcccatc ctgacggtga cggcggccct cggcggaagg cccgaaacct
     1441 caagcgacat gggcacaccc gcctccacgg cgggctgccc cgaccagtct ccgatcaggg
     1501 gctcagcctc atgaagcggc agcggcacgg gggccgcgcc ctcggggcgg accgtgagca
     1561 cgagcgttcc cgcctgcacg cccgcacctg catcttcagc agtcatggcc acctccgtca
     1621 gaagaagcgg tagcagtcca tggcctcgtc gccctgacgc agaatgcggt cgacgacctc
     1681 gacggacctc gacagtccga gcgactcaag accgctcctg aactccacga gcttctcgcg
     1741 gtaggcggcg cccgtgcccg tgatgcgcgg caggcacttc tgcatcaccg caaagtcggc
     1801 cgcatcaagc caggcgggct tgtccccgtc cgcaaagatc gaggacgccg cgcccacgta
     1861 tccgttcatc tggcggatcg agcggggcga gagcctgatg ccgaagcctt cgagcatggg
     1921 catcagcctg tcgtgcaggc ccgagagctc gcgcgcgttc ttcgaaatgg ccttcgcccc
     1981 gaagtaggcc tgaagctcct tccaggacac gggtcccacg accttcgcgg ggctcgtgtt
     2041 ttcaatgcaa tcgacgacgg gcagcgtcac gacggctgca cggtcgatga ggcgcgacga
     2101 gagcgtctcg gtcgtactgt cgttgttgat ggtcgccatg aagcgcagcg tctcgggcac
     2161 cttcgtcggc gccttgtccc agagcgtcac gatgccggac gacgtctgct cgtcgcacag
     2221 acgcatccag tcggaccagt agtactccat cggcgagagg ttcgcctcgt caagcagcat
     2281 catcgcggga tagaccgacc ccgaagcgct cctctcctcc gcatcaagcg tgcagagcag
     2341 gtcccatctg gcgggatccg aagactcgaa tctgttcgtg atcggattcc agtagcccac
     2401 gaagtcgcgc ttcgacgtcc agccgtactc gaccggcacg tgcacgaagc gtccggcgga
     2461 aagacccgca c
//
LOCUS       DBMDTI010000297         2470 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9361_length_2470_cov_2.661038, whole genome shotgun sequence.
ACCESSION   DBMDTI010000297 DBMDTI010000000
VERSION     DBMDTI010000297.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9361_length_2470_cov_2.661038"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(187..543)
                     /locus_tag="ACZPB6_09815"
     CDS             complement(187..543)
                     /locus_tag="ACZPB6_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024996.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR1184797.1"
                     /translation="MMFRLLPAAAMTATALATMTAGAATVDVYGVVDTGLHYVKADSG
                     DTSVTMDSGISKGSRVGLIGSEDLGGGYKIVFNLETGFSLDDGSFDNTKNRLVNPPRP
                     IINWTGLPCLLPARTA"
     gene            complement(570..1364)
                     /locus_tag="ACZPB6_09820"
     CDS             complement(570..1364)
                     /locus_tag="ACZPB6_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008864891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1184798.1"
                     /translation="MKKLALALLASLALSTGAQALETGVAPKVTELGVSNPTTGLYVG
                     NSYSFYNCGVHGYVRGLTRAANRTWKARLQTISSGMLSWHNVEDYLLPHEMDTYVVKG
                     TDKMFDVVFLQGMSSEPIAAKRVPTFRKYLAKHVETIRSKGSVPVVVVTWAKADKMDK
                     DTRRLADSIIEEANRNNAIALPVGLAFAESLKERPDLVLHQADKSHPTAAGSYLYGAL
                     IYSLLFKASPEGLDFLGECEKPLRPEDAAYLQKLAWRVATEFYGWK"
     gene            1577..2413
                     /locus_tag="ACZPB6_09825"
     CDS             1577..2413
                     /locus_tag="ACZPB6_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1184799.1"
                     /translation="MATKKKTDCRQYRIGDFARYLGVTAEFLKHYQESGLLDVTQRAS
                     GYRYYGFDQSARILQYMRLRNYGISVKEMGPFLEGGLDEAVGCLDAKVDEMRAQIERM
                     QAVVEEHERIRLWFEERRAKPVDWEVCNMEPHWFLYHTNSREFLETSCVYDVLKTWGA
                     WLPVTKSAMCVAQSLEIDESHLHWGFAVRESLLKKYGIPVNEAVRRMGFGKAFVFHFS
                     GIPNGFLMTDIVKGEHPAFQRMNALGFEQAGDALLVHELQLEAPGAKRRCCGRFIIPV
                     KD"
BASE COUNT          461 a          685 c          831 g          493 t
ORIGIN      
        1 atcggagctc ggtacttcct cagggagtac gagaagtcca tttcggcaac gagatggcgg
       61 tgcattgcgg ctaaagttcc gtcatgtgcc aagaggatca cgcgcaccct ggacactctc
      121 attcgggtta acgcggagct gcgctccatg tgagcgcaga tttctgagtt tgcccgctat
      181 ctgtgctcag gcagtccttg ctggtaaaag gcagggaagc cccgtccaat taataattgg
      241 gcggggagga ttcaccagac ggttcttggt gttgtcgaag gagccgtcgt cgaggctgaa
      301 gcccgtctcc agattgaaga cgattttgta tccgccgccg agatcctcgg agccgatgag
      361 gcccacgcgg gagcccttgg aaatgcccga gtccatggtc acggacgtgt cgccgctgtc
      421 ggccttgacg tagtgcaggc cggtgtcgac gacgccgtag acgtcaacgg tggcggcgcc
      481 ggccgtcatg gtggcgagcg ccgttgcggt catggccgca gcgggaagca gacgaaacat
      541 catgatgaat ctcctttatt ggtctcttgt catttccagc cgtagaactc cgtggcgact
      601 cgccacgcaa gcttctgcag gtaggcggcg tcctcgggcc tgaggggttt ttcgcattcg
      661 ccgaggaagt cgagtccttc gggcgaggcc ttgaagagga gcgagtagat gagcgcgccg
      721 tagaggtagg agcctgcggc ggtcggatgg ctcttgtccg cctgatgaag cacgaggtcg
      781 gggcgctcct tcaggctttc ggcgaaggca aggcccacgg gcagcgcgat cgcgttgttg
      841 cggttggctt cttcgatgat gctgtcggca aggcggcggg tgtccttgtc catcttgtcg
      901 gccttggccc aggtgacgac cacgacgggg actgagccct tggagcggat cgtctcgacg
      961 tgcttcgcga gatacttcct gaaggtcggc acgcgcttgg cggcgatggg ctcggacgac
     1021 atgccctgaa ggaagacgac gtcgaacatc ttgtcggtac ccttcacgac atacgtgtcc
     1081 atttcgtgcg gcaaaagata gtcctcgacg ttgtgccagc tcagcatccc ggagctgatg
     1141 gtctgcaggc gggccttcca ggtgcggttg gcggcgcgcg tgaggccgcg gacgtagccg
     1201 tgaactccgc agttatagaa cgagtacgag ttgccgacgt agagtccggt cgtcggattc
     1261 gaaacgccga gttcggtgac tttgggagcc acgcccgttt cgagcgcctg agcgccggtc
     1321 gagagcgcca gagaggcgag cagagccagt gcgagtttct tcatggtctt ctcctgttgt
     1381 ggtggtcgga ttcgggagcg gatcccgatt gacggcaatc tagcaaaggt gaggacggcc
     1441 cgaaacgcag acgcaggcaa agaaggtttg cgtggaacgc aagacgggca ccgtcgggac
     1501 gaggcgcttc gacagttctc cttcgaggcg tgagagaatg gaagttgcac ttcatgaaac
     1561 ggactcaggc tcaaccatgg cgacgaaaaa gaagactgac tgccggcagt accgcatcgg
     1621 cgactttgcc cgctacctcg gcgtgacggc cgaattcctc aagcactatc aggagagcgg
     1681 cctgcttgac gtcacgcagc gcgcgagcgg ctaccgctac tacgggttcg accagtcggc
     1741 ccgcattctt cagtacatga ggcttcgcaa ctacggcatt tccgtcaagg aaatgggacc
     1801 gtttctcgag ggcggtctcg acgaggccgt cggatgtctt gacgccaagg tcgacgagat
     1861 gcgcgctcag atcgagcgca tgcaggccgt ggtcgaggag cacgagcgca ttcgcctctg
     1921 gtttgaggag cgtcgtgcga agcccgttga ctgggaggtc tgcaacatgg agccgcactg
     1981 gtttctctac cacacgaatt ctcgcgagtt tcttgagacg tcgtgcgtct acgacgtcct
     2041 gaagacctgg ggcgcctggc tccccgtcac gaagtcggcg atgtgcgtcg cgcagtcgct
     2101 cgagatcgac gagtcgcacc tgcactgggg ctttgcggtg cgggagtcgc ttctgaagaa
     2161 atacggaatt cccgtcaacg aggctgtgcg ccgcatgggc ttcgggaagg ccttcgtctt
     2221 tcacttcagc gggattccga acggcttcct gatgaccgac atcgtgaagg gcgagcatcc
     2281 cgccttccag aggatgaacg cgctcggctt cgagcaggcg ggcgacgcgc tgctcgtgca
     2341 cgagctgcag cttgaggcgc cgggcgcgaa aaggaggtgt tgcggccgct tcatcattcc
     2401 cgtgaaggac tgatccggag tgtggcgcgg atgaaaacaa acgggggcgg ccgtgaggcc
     2461 gtccccgctt
//
LOCUS       DBMDTI010000298         2465 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9391_length_2465_cov_2.184010, whole genome shotgun sequence.
ACCESSION   DBMDTI010000298 DBMDTI010000000
VERSION     DBMDTI010000298.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9391_length_2465_cov_2.184010"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="ACZPB6_09830"
     CDS             <1..554
                     /locus_tag="ACZPB6_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184800.1"
                     /translation="QCWALSVPSELEASDKAAALARALEAVNTRDDAVMLVHPEGVFV
                     PESLLPVGWIEDPAEAGILLRVKAMPFAEGVRHMRASPIRRVRMTGSSASRAARYDPG
                     LRGAKDGSIELTGVVINRHSVRPVVRFVNGEGRTVEETEGHWPRVLDMEAIDRDPPKG
                     TEAFRALKRAVGWKPRERASDKA"
     gene            574..>2465
                     /gene="traD"
                     /locus_tag="ACZPB6_09835"
     CDS             574..>2465
                     /gene="traD"
                     /locus_tag="ACZPB6_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03743.1"
                     /note="Members of this protein family are the putative
                     conjugative coupling factor, TraD, as the term is used for
                     the SXT and TOL plasmid systems; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer system coupling protein
                     TraD"
                     /protein_id="HIR1184801.1"
                     /translation="MKTTEAVTSGVSSSRLTGWRPAWEGWSAAAGFAAAGTTAAMVPG
                     WWGAGLASVLALMGVVRTAEALRTASRRIPLAGFAPAFIHAGDLIRILREKGMDEKKA
                     VDPARKTARERLVARYEAGRIALLDSMRFNAGREGRFVKNLWIGRGFEWTPAHAQALY
                     ELAATPVKPVIVPKRIRKALSLPDPLGAGDIGSPIIHGIGATEEDDIVRPLSSLGGGT
                     LIVGTTQAGKGVMLTSLVTQAILRGEPVIVIDPKSSKRLHNAVWKAAEIAGRPAPLEF
                     HPAFPETGVRLDPLGAWTRPTELATRIAAVMPPDSGAFGNFAWMAVNVAVEGLFYVTE
                     RPSLLGLRRLLEGGIDPLLKKALARSFRDAGIDDWEEQVERMDKRNICPPAANAGIEL
                     TAAVALWEETVGKKDEGDANAVVGGLIAVFRHNREHYAKITASLQPVLSMLTGGTLAK
                     SFSPDPFDPDDERPIVTVERVVEGGDILYLGLDALPDSTVAGALGSIILADLTAYAGK
                     RYNRGESGTDVKSVSLFVDETANVINGPMIELLNKGLEAGIRVTAAMQTVSDLAARLG
                     SDSRARMALGNFNNLIALRSKDRLTQEFICETFGKTTVWSTSASLSSSADGSPVPDFR
                     ASVTRS"
BASE COUNT          455 a          850 c          751 g          409 t
ORIGIN      
        1 gacagtgctg ggctttgtcc gtcccctctg agctcgaagc ttccgacaag gcagcggcgc
       61 tcgcaagggc gctcgaagcc gtgaacaccc gggacgacgc cgtcatgctc gtgcatcccg
      121 agggcgtctt cgtccccgag agcctgctcc ccgtcggatg gatcgaggat ccggccgaag
      181 cagggatcct cctgagagtc aaggccatgc cctttgccga aggcgtacgt cacatgcggg
      241 cctcgcccat tcgccgcgtg cgaatgacgg gatcctccgc ctcgcgggcc gcccgatacg
      301 accccggcct caggggcgca aaggacggtt cgatcgaact cacgggcgtc gtcatcaacc
      361 ggcattccgt gcgccccgtc gtccgattcg tcaatggcga aggccgcacc gtagaagaaa
      421 ccgaaggcca ctggccgcgc gtcctcgaca tggaggcgat cgaccgcgat cccccgaaag
      481 gaaccgaagc cttcagggcc ctcaagcgcg ccgtcggctg gaagccgagg gaacgagcct
      541 cagacaaggc atgattgaag ggagaacaac acaatgaaga caacagaagc cgtcacgagc
      601 ggcgtatcct cctcgcgact cacgggctgg cggcccgcat gggagggctg gagcgcggcc
      661 gcgggatttg cagccgcagg gacaaccgcc gccatggttc cgggctggtg gggcgcgggt
      721 cttgcttccg tcctcgccct catgggtgtc gtgcgcaccg ccgaagcact ccggaccgcc
      781 tcccgccgca ttccgctcgc aggctttgcg cccgccttca ttcacgcggg cgacctcatc
      841 cgcattcttc gtgaaaaggg gatggacgag aagaaggctg tcgaccccgc aaggaaaacc
      901 gcccgcgagc gtcttgtcgc ccgctacgaa gcgggccgga ttgcgcttct cgactcgatg
      961 cgcttcaatg caggccgcga aggcaggttc gtcaagaacc tctggatcgg ccgcggattc
     1021 gagtggactc ccgcacacgc ccaggcgctc tatgaacttg ccgccacacc cgtcaagccc
     1081 gtgatcgtcc cgaagcgcat ccgcaaggcg ctctcactac cggacccgct cggggccgga
     1141 gacatcggaa gtcccatcat tcacgggatc ggtgcgaccg aagaggacga catcgtgcgt
     1201 ccgctctcga gtctcggcgg cggcacgctc atcgtcggca cgactcaggc agggaagggc
     1261 gtgatgctca cgagcctcgt cacccaggcg atccttcggg gtgaacccgt catcgtcatc
     1321 gaccccaaga gcagcaagcg tcttcacaac gccgtctgga aggcggccga gatcgcgggt
     1381 cgtcccgctc ctctcgaatt ccatcccgcc tttcccgaaa cgggcgtcag acttgatccg
     1441 ctcggcgcat ggacgcgtcc cacggaactt gccacccgca ttgcggccgt catgccgccc
     1501 gacagcgggg ccttcggcaa cttcgcctgg atggccgtca acgttgccgt cgaaggactc
     1561 ttctacgtga ccgaaaggcc gagccttctg gggcttcgcc gtcttctcga aggcggcatc
     1621 gatccgcttc tcaagaaggc tctggcccga tccttcaggg acgcagggat cgacgattgg
     1681 gaggagcagg tcgagcgcat ggacaagcgc aacatctgtc cgcctgccgc caatgccggc
     1741 atcgagctca ccgctgccgt cgccctctgg gaggagaccg tcggcaagaa ggacgaaggt
     1801 gacgcgaacg ctgtcgtggg cggtctcatc gccgtctttc gtcacaaccg tgaacactac
     1861 gcgaagatca cggcctcgct acagcccgtt ctctcgatgc tcacgggcgg cacgcttgcc
     1921 aagagcttct cgcccgatcc gttcgatccc gacgacgagc gtccgatcgt gacggtggag
     1981 agggttgttg aaggcggcga cattctctat ctcggccttg acgcgctccc cgactcgacc
     2041 gtcgccggcg cgctcggttc catcatcctc gccgacctca cggcctatgc cggcaagcga
     2101 tacaaccgag gcgaatcggg taccgacgtg aagtccgtct cgctcttcgt ggacgagacc
     2161 gccaacgtca tcaacggtcc catgatcgag ctcctcaaca aggggctcga agccggcatt
     2221 cgcgtcaccg cagccatgca gacggtgagc gatcttgcag caagactcgg gagcgacagc
     2281 cgcgcccgca tggcgctcgg caacttcaac aacctgattg cgctgagaag caaggaccgc
     2341 ctcacgcagg aattcatctg cgaaacgttc gggaagacca ccgtctggtc gacctccgct
     2401 tcgctctcct catcggccga cggctctccc gttccggact ttcgcgcctc cgtcacgcgc
     2461 agcat
//
LOCUS       DBMDTI010000299         2458 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9418_length_2458_cov_1.667374, whole genome shotgun sequence.
ACCESSION   DBMDTI010000299 DBMDTI010000000
VERSION     DBMDTI010000299.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9418_length_2458_cov_1.667374"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="ACZPB6_09840"
     CDS             <1..815
                     /locus_tag="ACZPB6_09840"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR1184802.1"
                     /translation="WCYVHDGAQSKRMHAGHAAWGGLMSAVLARRGFTGPTHVFEEVW
                     GGFNHSFALTSSDPDAYLRELGENWKLGRVSIKPHASCRSTHAAIDAVDNLRAAHGFT
                     ADDVEEVHVIINPFVYGMCGHATLHPMNSAQLSIPYSVAADLVFGSAGLPSFARARRE
                     DPRVAAMMARVRFTVDKSQKDDDEPIVEVTLKDGRSWREHVPMPLGAPTNPVTDEALL
                     RKFRGVVGMVFTEEETEELARTLMALEEVSDFRTEVASLLAREPRTREQFDA"
     gene            complement(1015..1674)
                     /gene="leuE"
                     /locus_tag="ACZPB6_09845"
     CDS             complement(1015..1674)
                     /gene="leuE"
                     /locus_tag="ACZPB6_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HIR1184803.1"
                     /translation="MLETFGILNIWAYALGALIIVMIPGPNTLFVLKTSIVDGRRAGF
                     TAAAGVFTGDAVLVFLAYLGIAALVLAHPEVFFWLKILGAAYLAWLGGKAIHATFFAK
                     KAEKAEGESEVVVKKSRSLVKVYRNALALSLTNPKSILFYVSFFVQFIDPAFANPAIS
                     YTILALILECFSMAWMTLLITAGADLLKFFGRRPSVAKLGNTAMGSMFLLFASKLALD
                     A"
     gene            complement(2146..>2458)
                     /locus_tag="ACZPB6_09850"
     CDS             complement(2146..>2458)
                     /locus_tag="ACZPB6_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184804.1"
                     /translation="SKILTDPLVFASRAWISVERHLYEGGSPRFISSGRGLHSFEGKT
                     NRTGIIWKADPQCVTVCKHVYRVRVAKHDDWLTRALQDPTDPTKPRKVKYCPSRSNSL
                     A"
BASE COUNT          484 a          722 c          802 g          450 t
ORIGIN      
        1 tatggtgcta cgtgcacgac ggcgcgcaga gcaagcgcat gcatgcgggc catgcagcct
       61 ggggaggtct gatgagcgcc gtgctcgcac gccgcggctt cacggggccg acgcacgtct
      121 tcgaggaggt ctggggcggc ttcaaccatt ccttcgcgct gacctcgagc gatccggatg
      181 cttatctgcg cgaactcggc gagaactgga agctcgggcg cgtctccatc aagccgcacg
      241 cctcctgccg ctccacccat gccgcaatcg acgccgtcga caacctgcgc gccgcacacg
      301 gcttcacggc cgatgacgtc gaggaggtgc acgtgatcat caatccgttc gtctacggca
      361 tgtgcggtca cgcgacgctt catccgatga actcggccca gctctccatc ccctacagcg
      421 tggcggccga cctcgtcttc ggctccgcgg gccttccctc gtttgcgcgc gcccggcgcg
      481 aggacccgcg cgtcgccgcc atgatggcgc gcgtgcgctt cacggtcgac aagagccaga
      541 aggacgacga cgagccgatc gtcgaggtga cgctcaagga cggccgcagc tggcgcgaac
      601 acgtgccgat gccgctcggc gcaccgacca atcccgtcac tgacgaggcg cttctcagga
      661 agttcagggg cgtggtcggc atggtcttca ccgaggagga gaccgaggag ctcgcacgca
      721 cgctcatggc gctcgaagag gtctccgact tcaggaccga ggtggcgagc ctgctcgccc
      781 gcgagccgag gacgcgcgag cagttcgacg cataaagtgc tcgaacatgg tgtgataaaa
      841 tcacggggtc gcaacctgct ctcggatggt tcagcagtca ccgcgcgagt tgcagatgac
      901 gacggcctcg gcccggtaga cgtcttcgaa attctgggag aggcaaaaca agacggcctg
      961 cattccacat tcggattgca ggccgtctta tttggtgctt gtcccgtcgg agcgtcaggc
     1021 gtcgagcgcg agcttcgatg caaagagcag gaacatgctg cccatggcgg tgttgccgag
     1081 cttcgcgacg gacggacgac ggccgaagaa cttgagaagg tcggcgcccg cggtgatgag
     1141 gagcgtcatc caggccatgc tgaagcattc aaggatcagg gcgaggatgg tgtaggagat
     1201 cgccggattg gcgaaagccg gatcgatgaa ctgcacgaag aaggagacgt agaagagaat
     1261 cgacttcggg ttcgtgaggc tcagcgcaag ggcgttgcgg tagaccttga cgaggctgcg
     1321 gctcttcttg acgacgactt cggattcgcc ttcggccttt tcggccttct tggcgaagaa
     1381 cgtggcgtgg atggccttgc cgccgagcca ggcgaggtac gcggcgccga ggatcttgag
     1441 ccagaagaag acctcggggt gcgcgagcac gagggccgcg atgccgaggt aggcgaggaa
     1501 gaccagaacg gcgtcgcccg tgaagacgcc cgcagcggcg gtgaagcccg cgcggcggcc
     1561 gtcgacgatg ctcgtcttga gcacgaagag cgtgttcggg ccggggatca tgacgatgat
     1621 cagagcgccg agcgcatagg cccagatgtt gaggatgcca aaagtttcaa gcatgatgga
     1681 gatgatccgt gcgaaaaaaa gaccgcactc cctcggagtc cggccggcag atgaattttt
     1741 gcggttggga cgcattcgac cccgcgggcc ttcctgcatc gagcgacgaa gtatacaggt
     1801 tttcgcctag gggacaaccc cgaaatgaga cattttccgg agaatttcct tcatgcggaa
     1861 ggtcgaaacc gtgaagacaa ttgcggccgt ggcgaagcgg ggcgattgtt gtccggatac
     1921 cgcgtttgcg ggacttgcta aacaacctcc ccactgaagt gggagggttt cggtacagtc
     1981 gaggcttttc gtctcggggt tgtccccgat ttgctgtcac tccgaccacg aaatccctgc
     2041 tgacgcagcc cttgtacggc aagggaagga atctcccgcc ctcggaacct cccgaactcg
     2101 cgctcggtga tgccttctcg agcttgaatg actacgacac cggcgtcaag ccaggctgtt
     2161 actccggcta ggacagtact tcacctttct gggctttgtg ggatccgtcg gatcctgcaa
     2221 agctcgggtg agccagtcgt cgtgcttggc aacgcgaaca cggtacacgt gcttgcagac
     2281 ggttacgcat tgcggatctg ctttccatat gatgccggtg cgattggtct tcccttcgaa
     2341 cgaatgcagt ccgcgtccag aactgatgaa cctcggggag ccgccttcgt agaggtggcg
     2401 ctccaccgag atccacgctc tcgaagcgaa tactaaagga tcggtcagga tcttggag
//
LOCUS       DBMDTI010000300         2447 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9469_length_2447_cov_2.019608, whole genome shotgun sequence.
ACCESSION   DBMDTI010000300 DBMDTI010000000
VERSION     DBMDTI010000300.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9469_length_2447_cov_2.019608"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(131..952)
                     /gene="trpA"
                     /locus_tag="ACZPB6_09855"
     CDS             complement(131..952)
                     /gene="trpA"
                     /locus_tag="ACZPB6_09855"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR1184805.1"
                     /translation="MTERFENLFARLAQRREGAFVPFVNLCDPDPGTSLAVLETLVAS
                     GADALELGIPFSDPCADGPVVEASADRALANGATPARCLDVLRRFRGAHPETPVCLML
                     YINLVAAPDVRRFMQAAADAGADAVLIPDLPTSMREAEPEWDEAAREAGLHLVAIVPP
                     NASDERVARIAGLTSGYTYLLSRVGITGTDHASGTPAERIIRDLERAKAPPTLLGFGI
                     STPGHVRRALEAGAAGVIVGSALVKIVSEHLGDRDAMLKALGSAAASFKAATRTA"
     gene            complement(1009..2307)
                     /gene="trpB"
                     /locus_tag="ACZPB6_09860"
     CDS             complement(1009..2307)
                     /gene="trpB"
                     /locus_tag="ACZPB6_09860"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR1184806.1"
                     /translation="MPPTQTRKPNTLNYTEPNSKKESPSCCSIPTSANTAASSSPSFS
                     FPPSTSSSAPTSTRRTIPPSGRLLTTYAGRPTPLTRLTRLTEGTKTTIYLKREDLLHG
                     GAHKTNQVLGQALLTKRMGKTRVIAETGAGQHGVATALASALFGFKCRIYMGAKDVER
                     QKPNVFRMELMGAEVVPVTAGSGTLKEACNAALRNWAESFEDTHYMLGTAAGPHPFPT
                     IVREFQKVIGEEARAQFAEAENGLLPDAVIACVGGGSNAIGLFTDFRPFEDTRLIGVE
                     PAGMGIASGKHGVTLGEGQLGIFFGARSYNMQTPEGQINESYSISAGLDFPSVGPEHA
                     HLKYTGRAEYVSVTDREALGAFLELSLKEGIIPALESSHALAYALKMARAEPEKEQKL
                     VVNLSGRGDKDIFQVAARLEADGCLKDGRLSSDALGAYAD"
     gene            complement(2307..>2447)
                     /gene="trpF"
                     /locus_tag="ACZPB6_09865"
     CDS             complement(2307..>2447)
                     /gene="trpF"
                     /locus_tag="ACZPB6_09865"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012900.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR1184807.1"
                     /translation="AGGLTPENARTAAAAGAAGLDANSGVESAPSLKDPRLLALFAGA
                     VR"
BASE COUNT          343 a          731 c          916 g          457 t
ORIGIN      
        1 accgcgttgc aggcgtttag gcgctgtttc ttgtgaagaa ggcagggcgc cagcagaagt
       61 tttcgtctgc gggtgcccct ttttaataat ctaaaaacat ttggaatact catgcgtggt
      121 ccatggttct tcaggccgtt cgcgtggcgg ccttgaagga ggcggcggcg ctgccgaggg
      181 ccttgagcat ggcgtccctg tcgccgaggt gttcggagac gatcttcacg agggcggagc
      241 ccacgatgac gcctgcggcg cccgcttcga gcgcgcgcct gacgtgtccg ggcgtggaga
      301 tgccgaagcc gagcagggtg ggcggggcct tggccctctc gaggtcgcgg atgatgcgct
      361 ctgcgggcgt gcccgaggcg tggtcggtgc cggtgatgcc gacgcgcgag agcagatagg
      421 tgtatccgct cgtgaggccc gcgattcttg cgacgcgctc gtcggaggcg ttgggcggga
      481 cgatggccac gaggtgcagg cccgcctcgc gggcggcctc gtcccattcg ggctcggcct
      541 cgcgcatgct cgtcgggagg tcgggaatga gcacggcgtc tgcgcccgcg tcggctgcgg
      601 cctgcatgaa gcgcctgacg tcgggcgccg cgacgaggtt gatgtaaagc atgaggcaga
      661 cgggcgtctc gggatgtgcg cccctgaacc tgcgcagcac gtcgaggcag cgtgcgggcg
      721 tggcgccgtt ggcgagggcc ctgtcggcgg aggcctcgac gacggggccg tcggcgcacg
      781 ggtcggagaa cgggatcccg agctctagcg cgtcggcgcc cgaggcgacg agcgtttcga
      841 gaacggcgag cgaggtcccc ggatcggggt cgcagaggtt cacgaagggg acgaatgcgc
      901 cctcgcgcct ttgggcgagt cgggcgaaga ggttttcaaa gcgttcggtc atggcgtctc
      961 tcattcagca gtgtttccgt tgtgtgttcc gtagtctttt tcggcatctc agtcggcgta
     1021 ggccccgagt gcgtcggagc tgaggcggcc gtccttcagg cagccatcgg cctcgagcct
     1081 cgcggccacc tggaagatgt ccttgtcgcc tcggcccgag aggttgacga cgagcttctg
     1141 ttccttttca ggttccgcgc gggccatctt gagcgcgtag gcgagggcgt gggagctttc
     1201 gagcgcgggg atgatgcctt ccttgaggga gagttcgagg aaggcgccga gggcttcgcg
     1261 gtcggtgacg ctcacgtact cggccctgcc cgtgtacttg aggtgggcgt gttcggggcc
     1321 gaccgacggg aagtcgaggc cggccgagat cgagtacgac tcgttgatct ggccttctgg
     1381 cgtctgcatg ttgtaggagc gcgcgccgaa gaagatgccg agctgaccct cgccgagcgt
     1441 gacgccgtgc ttgcccgagg cgatgcccat gcccgcgggc tcgacgccga taaggcgggt
     1501 gtcttcgaag ggcctgaagt ccgtgaagag gccgatggcg ttggagcccc cgcccacgca
     1561 ggcgatgacg gcgtcgggca ggagtccgtt ctcggcctcg gcgaactggg cgcgggcctc
     1621 ctcgccgatc accttctgga actcacgcac gatcgtcggg aagggatgag ggcccgcggc
     1681 ggtgccgagc atgtagtgcg tgtcctcgaa ggattcggcc cagttgcgaa gggccgcgtt
     1741 gcaggcctcc ttgagcgtgc cgcttcccgc cgtgacgggc acgacctcgg cgcccatgag
     1801 ctccatcctg aagacgttgg gcttctggcg ctcaacgtcc ttggcgccca tgtagatcct
     1861 gcacttgaag ccgaataggg ccgaggcgag cgccgtggcg acgccgtgct ggcccgcgcc
     1921 cgtctcggcg atcacgcggg tcttgcccat gcgcttcgtg aggagcgcct ggccgagcac
     1981 ctggttcgtt ttgtgggcgc cgccgtgcag caagtcctcg cgcttgaggt agatcgtcgt
     2041 cttcgtgccc tccgtaaggc gcgtgagcct cgtgagcggc gtcggacggc ccgcataggt
     2101 cgtcagcagg cgtcctgaag gcgggatcgt ccgtcgcgtc gacgtaggcg cgctcgagct
     2161 ggtcgagggc gggaaggaga agctcgggga cgaactggcc gccgtattgg ccgaagtagg
     2221 gattgagcag catgatggag attccttttt cgagttcggt tctgtgtagt tcagtgtgtt
     2281 cggttttcgg gtctgcgtcg ggggcatcac cgcacggcgc ccgcgaagag ggccagaagg
     2341 cgcggatcct tgagactcgg cgccgactcg acgcccgagt tcgcgtcgag ccccgccgca
     2401 cccgcagccg cggcggtccg tgcgttttcg ggcgtgagtc cgcccgc
//
LOCUS       DBMDTI010000301         2416 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9638_length_2416_cov_2.885529, whole genome shotgun sequence.
ACCESSION   DBMDTI010000301 DBMDTI010000000
VERSION     DBMDTI010000301.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9638_length_2416_cov_2.885529"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="ACZPB6_09870"
     CDS             complement(<1..1407)
                     /locus_tag="ACZPB6_09870"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1184808.1"
                     /translation="MSRLSILRTLCAHWRTSILMRTVAVAATATVALTLVTAAVVFQG
                     AEKDALVEQDARLSDIAVAMARGELGTVLPRALTMPQAQFEARLESDEPLPRPRRMHR
                     HMMAHCPTAASDDPNAPRLIPAGSPVYVRMLNDQGRAKRAVFDEPVEGGLTTRTIKGE
                     TYRMAVLFLPGGRYTAVADPVSTREVYARSAAMTAVLPILILLPVLVIVIGLTLWRML
                     APIRSAARAAAERPATDLTPLPSEGVPAEVKPFVEAVNSLLGRVSEARTRELRFTADA
                     AHELRSPLTSLTIEAEHLGRLDLPAEARPVVARLESGLERAVKQVSQLLQFARAQAGE
                     APEILRRDTRPWYLSELLGEILEPLMNCAADKDLQFDVEGLDDGAEAPVEGVSRAALL
                     AILRNLLENAFHYTPAGGSVTLRVSRTPERLVMSVADTGPGIAPEERSRVFDPFYRVT
                     GSGLPGTGLGLAIVKTYAD"
     gene            complement(1427..2110)
                     /locus_tag="ACZPB6_09875"
     CDS             complement(1427..2110)
                     /locus_tag="ACZPB6_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1184809.1"
                     /translation="MHILLVEDDPFIAKAVCAALESEAHAVTWTPTGREGLAVIATGT
                     PDAVLLDLGLPGMDGIDVLKSIRAMTKPLCDTPVIIVTAREALESRLEGLDAGADDYI
                     LKPFHMSELIARLRAVVRRRSPLAPDVLRAGSLSLNTVTHACSVGSESVTLSKREYAL
                     LAALMTRPGAILSRRALEEKLYAPGDEPESNAVEYLIHSLRKKIGASAIENIRGLGWR
                     VPADKNAAQ"
     gene            complement(2237..>2416)
                     /locus_tag="ACZPB6_09880"
     CDS             complement(2237..>2416)
                     /locus_tag="ACZPB6_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184810.1"
                     /translation="TGKVVAADVKGIQERRPGRGMGPGPRPQCPVTGGAPGTNPDCPM
                     AGGFRHPGFHGYHHW"
BASE COUNT          377 a          730 c          932 g          377 t
ORIGIN      
        1 gtcggcgtag gtcttcacga tcgcaaggcc gaggcccgtg cccggaagtc cggaacccgt
       61 gacacggtag aagggatcga agacgcgcga acgctcctcg ggcgcaatgc cggggcccgt
      121 gtcggccacg ctcatcacga ggcgctcggg cgtgcgcgag acgcgaagcg tcacggatcc
      181 gcccgcgggc gtgtagtgga aggcgttttc aagaaggttt cgcagaatcg ccagcagggc
      241 cgcccggctc acgccttcga cgggcgcttc ggcgccgtcg tcaaggccct cgacgtcgaa
      301 ctgcaggtcc ttgtcggcgg cgcagttcat caggggctcg agaatctcgc ccagaagttc
      361 cgagaggtac cagggacgcg tgtcgcgcct gaggatttca ggagcctcgc ccgcctgcgc
      421 gcgtgcaaac tgcaggagct gcgagacctg cttgacggcc cgctcgagtc cggactcaag
      481 acgagccacg acgggacgcg cttcggcggg caggtcgagg cggccgaggt gttcggcctc
      541 gatcgtgagc gacgtgagcg gcgagcggag ctcgtgcgca gcatcggctg tgaagcggag
      601 ctcgcgcgtg cgcgcttcac tcacgcgtcc gagcaggctg ttgacggcct cgacgaaggg
      661 cttcacctcg gcgggaacgc cctcggaagg caggggcgtg aggtcggtgg cgggacgctc
      721 ggccgcggct cgggcggcgc ttcggatcgg cgcgagcatg cgccagagcg tgaggccgat
      781 cacgatgacg aggaccggca gaagaatcag aatcgggagc acggccgtca tggcggcgct
      841 gcgggcgtag acctcgcgcg tcgagacggg atcggccacg gccgtgtagc ggcccccggg
      901 aaggaagagc acggccatgc ggtaggtctc gcccttgatg gtgcgggtcg taaggccgcc
      961 ctcgacgggt tcgtcgaaga ccgcgcgttt ggcgcgtccc tggtcgttga gcatgcgcac
     1021 gtagacgggg gagcccgcgg gaataagacg gggcgcgttc ggatcgtcgg aagcggccgt
     1081 cgggcagtgg gccatcatgt ggcggtgcat gcggcgcggg cggggcaggg gctcgtcgga
     1141 ttcaagtctg gcctcgaact gggcctgcgg catcgtgagc gcgcgcggaa gcacggttcc
     1201 gagttcgcct cgggccatgg cgacagcaat gtcggagagc cgggcgtcct gctcgacgag
     1261 ggcgtccttt tcggcgccct gaaagacgac ggcggcggtc accagggtga gcgcaaccgt
     1321 cgccgtggcg gcgaccgcca cggttcgcat gaggatcgag gtgcgccagt gtgcgcagag
     1381 agtgcggagg atgctcaggc ggctcatcgg gacgtccgtc ggtggattat tgtgcggcgt
     1441 tcttgtcggc gggcacgcgc cagcccaggc cgcggatgtt ttcaatggcc gaagcgccga
     1501 tcttcttgcg aagcgagtgg atcaggtatt cgactgcatt gctctcgggc tcgtcgcccg
     1561 gcgcatagag cttctcttcg agggcgcgcc tcgagaggat ggcgcccggt cgcgtcatga
     1621 gggccgcaag cagcgcgtac tcgcgcttgc tgagcgtgac ggattcgctt ccgacgctgc
     1681 aggcgtgggt gacggtgttg agcgagaggg agcctgcgcg cagcacgtcg ggtgcgagcg
     1741 gcgagcggcg gcgcaccacg gcgcgaaggc gcgcgatgag ctccgacatg tggaagggct
     1801 tgaggatgta gtcgtccgcc cctgcatcga gtccctcaag gcggctttca agcgcctccc
     1861 tcgccgtcac gatgatgacg ggcgtgtcgc agaggggctt cgtcatggcg cggatgctct
     1921 tgaggacgtc gatgccgtcc atgccgggga gtcccagatc gaggagcacg gcgtcgggcg
     1981 tgccggttgc gatgacggcg agcccctcgc ggcccgtcgg ggtccacgtg acggcgtgcg
     2041 cctcgctttc gagcgccgcg cagacggcct tggcaatgaa cggatcgtct tcgacaagaa
     2101 ggatgtgcat gatggcggac ggacgcgcga agcgcccctg agtgcgtttc ggaaacggaa
     2161 aacgggagga ctttcccctg aggaaggccc tcccgcgtga gtatatcagc ggcggttcag
     2221 gccgcttggg cagaggttac cagtgatggt agccgtggaa gcccgggtgg cggaagccgc
     2281 cggccatcgg acagtcgggg ttcgtgcccg gggcgccgcc cgtcacgggg cactgcggac
     2341 gcgggccggg acccatgccg cggccagggc ggcgttcctg aatgcccttg acgtcggcgg
     2401 cgacgacctt gccggt
//
LOCUS       DBMDTI010000302         2411 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9659_length_2411_cov_3.938528, whole genome shotgun sequence.
ACCESSION   DBMDTI010000302 DBMDTI010000000
VERSION     DBMDTI010000302.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9659_length_2411_cov_3.938528"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="ACZPB6_09885"
     CDS             complement(<1..728)
                     /locus_tag="ACZPB6_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1184811.1"
                     /translation="MAQYVFTMNRVGKIVPPKRQILKDISLSFFPGAKIGVLGLNGAG
                     KSTLLKIMAGVDKDIEGEAIPMPGINIGYLPQEPALDPAKTVRETVEEGMGEVMQAQE
                     KLNAVYDAYADPDADFDALAAEQARLEAIIAAAGTNVETQMEIAADALRLPPWDAVIG
                     NLSGGEKRRVALCRLLLSRPDMLLLDEPTNHLDAESVEWLEQFLHRFPGTVVAVTHDR
                     YFLDNAAEWILELDRGEGIPWKGN"
     gene            complement(942..1592)
                     /locus_tag="ACZPB6_09890"
     CDS             complement(942..1592)
                     /locus_tag="ACZPB6_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HIR1184812.1"
                     /translation="MTETKPFARADLAQAPTKLLRNGRVANAVVTRVEIDGRSWTVKD
                     FSARPWWVRTFIAPFLLRRELSILARLRGVEGISQESFRIDREAMAVLFMEGSNIGRE
                     PERVTPAYLEAFEELLRAMHARGVVHLDVRGTGNVMIRPNGSPGLIDFQASLYTGWMP
                     GFLRRLLEDFDMSGALKKWLQFQPEMMGEERRKELERINGLRKFWIFRGYFGLKKH"
     gene            complement(1641..2036)
                     /locus_tag="ACZPB6_09895"
     CDS             complement(1641..2036)
                     /locus_tag="ACZPB6_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184813.1"
                     /translation="MPAQDSIDAMNTQMDVASLYREEVVTDRKVGTIHVLTPILDSGE
                     RDTNRPLLFLGDTQIMTQMGPLPISFEIKAETLAEAVAGYGEAAKQGVRDTIEKLQAM
                     RREAASQIVTPGMPGFQAPPAAGSGIAMP"
     gene            complement(2194..>2411)
                     /locus_tag="ACZPB6_09900"
     CDS             complement(2194..>2411)
                     /locus_tag="ACZPB6_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR1184814.1"
                     /translation="KRRCDEIGVPSTTLMVKSFSPAEAIIEQSKKHDCDVVFMASHGR
                     QGFAAVLLGSETQKVLTHSQIPVMVYR"
BASE COUNT          445 a          704 c          831 g          431 t
ORIGIN      
        1 tagttgccct tccagggaat gccttcgccg cggtcgagtt cgagaatcca ttcggccgcg
       61 ttgtcgagga agtagcggtc atgggtcacg gcgacgacgg taccggggaa gcggtgcagg
      121 aactgctcga gccattcgac ggattcggcg tcgagatggt tcgtcggttc gtcgagcagc
      181 agcatgtcgg gacgagagag caacaggcgg cagagcgcga cgcggcgctt ttcaccaccg
      241 gagaggttgc cgatcacggc atcccaaggc ggaaggcgca gggcgtcggc ggcgatctcc
      301 atctgagtct cgacgttcgt gcccgcagcg gcgatgatgg cctcaagacg ggcctgctcg
      361 gcagcaaggg cgtcgaagtc ggcgtcggga tcggcatagg cgtcgtagac ggcgttgagc
      421 ttctcctgag cctgcatcac ctcgcccatg ccctcctcga cggtctcgcg gacggtcttg
      481 gccggatcaa gagcgggttc ctgcggcagg tagccgatgt tgatgccggg catcgggatg
      541 gcttcgccct cgatgtcctt gtcgacgccc gccatgatct tgaggagcgt ggacttgccg
      601 gcgccgttga ggccgaggac gccgatcttg gcgcccggga agaacgagag ggaaatgtcc
      661 ttaagaatct gacgcttcgg cgggacgatc ttcccgacgc ggttcatcgt aaaaacatac
      721 tgggccatgg tttgatgact actccggaca tgacgaaggc ggccggagaa cgaaggattc
      781 gtccgaacgc aacgtgcatg agctgataag ggaaaaaatg aggcgcggac cgacggatgc
      841 cggacgcatg gaatgtgccg ctatcttaac ggaaacggcg gggcttggcg acaaaaaagc
      901 cgcggactct gccgcggctc ttgtgagggt ttgcttcaga cctagtgctt cttgaggccg
      961 aagtaacccc tgaagatcca gaactttctt agcccgttga tgcgctcgag ctcctttcgg
     1021 cgctcctcgc ccatcatttc aggctggaac tgaagccatt tcttgagggc gcccgacatg
     1081 tcgaagtcct cgagaaggcg acgaaggaag cccggcatcc agcccgtgta gagcgaagcc
     1141 tggaagtcga tgaggccggg cgagccgttg gggcggatca tcacgttgcc cgttccgcgc
     1201 acgtcgagat gcacgacgcc gcgcgcatgc atggcgcgca ggagctcctc gaacgcctca
     1261 agataggcgg gcgtgacgcg ctcgggttcg cggccgatgt tcgagccctc catgaagagc
     1321 acggccatcg cctcgcggtc gatgcggaag gactcctggg aaatgccctc gacgccgcgc
     1381 aggcgcgcga gaatcgaaag ctctcgcctc agcaggaagg gcgcgatgaa ggtgcggacc
     1441 caccagggac gcgcggagaa gtccttcacg gtccaggagc gtccgtcaat ctccacgcgc
     1501 gtgacgacag cgttggccac gcggccgttg cgcaggagct tcgtcggagc ctgtgccaga
     1561 tcggcacggg caaaaggctt ggtctcggtc atgaggtatc ccggagaggc cgcccgaagg
     1621 cggcgcgtcc cctgacgtca tcagggcatg gcgatgccac tgccggcggc aggcggcgcc
     1681 tggaagcccg gcatgcccgg ggtgacgatc tggctggcgg cctcgcggcg catggcctga
     1741 agcttttcga tcgtgtcgcg cacgccctgc ttggctgcct cgccgtagcc ggcgacggct
     1801 tcggcaagcg tttcggcctt gatctcaaag gaaatcggca gcggacccat ctgggtcatg
     1861 atctgcgtgt cgccgaggaa gaggagcgga cggttcgtgt cgcgctcgcc ggagtcgaga
     1921 atcggcgtca agacgtggat ggtgccgacc ttgcggtccg tcacgacctc ttcgcgatag
     1981 aggctggcca catccatctg ggtgttcatg gcatcaatgc tgtcctgtgc gggcataaga
     2041 ctctccgaat ggccgcaggc gttgcgccgg cggcgtcatg ttccaatcaa tatccaatca
     2101 atgggaagcc ggcggcaggc cgccgacgtc gcgtcgaaat tgtacagaag ttgacgggac
     2161 ggtccgcagg gccgtcccga aggcgtcgtc agatcagcgg tagaccatga cgggaatctg
     2221 ggagtgcgtg agcaccttct gggtttcgga gccgagcagg acggccgcga agccctgacg
     2281 gccgtggctc gccatgaaga cgacgtcgca gtcgtgcttc ttggactgtt cgatgatcgc
     2341 ctcggcgggc gagaagctct tcaccatgag ggtggtcgag ggtaccccga tttcatcgca
     2401 gcggcgcttg g
//
LOCUS       DBMDTI010000303         2404 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9685_length_2404_cov_4.210161, whole genome shotgun sequence.
ACCESSION   DBMDTI010000303 DBMDTI010000000
VERSION     DBMDTI010000303.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9685_length_2404_cov_4.210161"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="ACZPB6_09905"
     CDS             <1..975
                     /locus_tag="ACZPB6_09905"
                     /inference="COORDINATES: protein motif:HMM:NF015251.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1184815.1"
                     /translation="WLHGRWRPIGFMSGLGLNPIKLIVWITSLEWTQAMDWLLKLFSR
                     LPLRFLQVVGAGIGGLTFKLSPATRARAMENLKLAGYDDPHLYERVGRNAGRQAMESL
                     WVWYRPAETVLKRVHIAPEAEAMVREAVDSGRPIVFLTPHVGCFEVLPVWFAATFFAR
                     TGRGISILYRPPKVSLLRKIVGEARQAPGIQACPTTIAGVKKMIRNLKQGHTFGALPD
                     QVPSKGEGVWADFFGRPAYTMTLPVRVAHQFKADRFFVWGVRSGDGWRIEAVKWDEPL
                     TGDTEKDAEAMNRQIEAVIRRMPEQYAWSYNRYKTPAGAHPKQAGDKK"
     gene            972..1850
                     /locus_tag="ACZPB6_09910"
     CDS             972..1850
                     /locus_tag="ACZPB6_09910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1184816.1"
                     /translation="MNVFFAKFWIRVVRSMKNLPMSVREALAKVIARILWLAIPKRRH
                     VIETNLRLCFPELSESERRSLAHRVYVRLARAAVDHGTLWESDKETIQKFVTFEGLEN
                     LLENKGEPIIVVAPHFAGLDAAGIALNTYVRGVSLYQKQSNPAWDEAVLAGRKRFSDP
                     VLIAKSNESDLRPVLRAIKEGLPFYYLPDMDHGIKNSIFVPFFGVQAATLPMVSRLAR
                     VTRAKVLWCIATMTEEGYHVIISKPWENFPTADFEADTRRLNAELEEWIRAYPDQYLW
                     VHRRFKTRPEGEQSLY"
     gene            1875..>2404
                     /gene="dapF"
                     /locus_tag="ACZPB6_09915"
     CDS             1875..>2404
                     /gene="dapF"
                     /locus_tag="ACZPB6_09915"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR1184817.1"
                     /translation="MRTLKFTKMQGAGNDFVMLDGIRQDIEPTPALIRYLADRRFGIG
                     ADQVLMVERARLPGVDFRYRIFNCDGDEVEQCGNGARCFAVFVREEGLTDKTSIRVET
                     MKAVIEPEVRPDGRVTVNMGPARKAPEVLPFVPEGLESGTEGASRIYHAHLSCSDVWF
                     SALSMGNPHAVIRVEDV"
BASE COUNT          472 a          671 c          728 g          533 t
ORIGIN      
        1 tggctgcatg ggcggtggag gcccattggg ttcatgtcgg gactgggact aaatccgata
       61 aaattaattg tttggattac ttctctcgaa tggacgcaag ctatggactg gttgctgaag
      121 ctcttttcaa ggctccctct gcgttttttg caggtcgtgg gggcgggcat cggaggcctg
      181 accttcaagc tgtccccagc caccagggcc cgggctatgg aaaatctgaa gctggctggt
      241 tatgacgatc ctcatctgta tgaacgagtc ggtcgcaatg cgggccgaca ggccatggag
      301 agcctttggg tttggtaccg tcctgccgag actgtgctca agcgtgttca tatcgcgccg
      361 gaagccgagg caatggtgcg cgaggccgtt gacagtggtc gcccgattgt ttttctgacg
      421 ccacacgtcg gttgctttga ggtgcttccc gtgtggtttg ccgccacgtt ttttgcaagg
      481 acggggcgcg gcatttccat tctttaccgc ccgcccaagg tgagtcttct cagaaagatc
      541 gtgggcgagg cgcgtcaggc tcccggcatt caggcttgcc cgacgacgat tgcgggtgtc
      601 aagaagatga tccgcaacct gaagcaggga catacattcg gtgcgttgcc tgatcaggtt
      661 ccttccaagg gggagggcgt ctgggccgac tttttcggca ggcctgccta cacgatgacg
      721 cttcctgtgc gtgtcgcgca tcagttcaag gctgatcgct tcttcgtctg gggcgtgaga
      781 agcggcgacg gctggcgcat tgaggctgtg aagtgggatg aaccgcttac aggtgacacc
      841 gaaaaggacg ccgaggcgat gaaccgccag atcgaggccg tcattcgtcg catgcctgag
      901 cagtatgcct ggagctacaa ccgctacaag acccctgcag gagcgcatcc caagcaggcc
      961 ggagacaaaa agtgaacgtt ttctttgcca agttctggat ccgtgtggtt cggtcgatga
     1021 agaatctgcc gatgtccgtg cgcgaagctc ttgcgaaagt cattgccaga atcctctggc
     1081 tcgccattcc gaagcgtcgc catgtcatcg agacgaacct gaggctctgc tttcccgagc
     1141 tttccgagtc cgagcgtcgc tcgctggcac atcgtgtcta tgtccgcctt gcgcgcgctg
     1201 ccgtcgatca cggcacgctt tgggaatccg acaaggagac gattcagaag ttcgtgacct
     1261 ttgaggggct tgaaaacctg cttgaaaaca agggtgagcc catcattgtt gtggcaccgc
     1321 atttcgcagg tctcgacgct gcaggcattg cgctcaacac ctacgtgcgc ggtgtgtcgc
     1381 tttatcagaa gcagagcaat cctgcctggg atgaggcagt gctcgcaggg cgcaagcgct
     1441 tctccgaccc tgtcctcatc gccaaaagca acgagagcga tctgcgcccc gtgttgcgcg
     1501 ccatcaagga ggggcttccc ttctattacc tgcccgacat ggaccacgga atcaagaact
     1561 cgattttcgt gccgttcttc ggcgtacagg cagccacgct gccgatggtc tcgcgtcttg
     1621 cgcgagtgac gagggcaaag gtcctctggt gcattgccac gatgaccgaa gagggctacc
     1681 acgtcatcat ctccaagccg tgggagaact tcccgacggc cgacttcgaa gcggataccc
     1741 gaaggctcaa tgccgaactc gaggaatgga ttcgtgcata tcccgaccag tacctctggg
     1801 tccaccgccg cttcaagact cgacctgagg gcgagcagtc actctattaa aaacgactga
     1861 aaagaatacc tgaaatgaga acgctgaagt ttacgaaaat gcagggtgcc ggcaatgact
     1921 tcgtgatgct tgacggcatc cgacaggaca tcgagcctac gcctgcactc atccgctatc
     1981 tggccgaccg tcgcttcggc attggggctg atcaggtgct gatggtcgag cgtgccaggc
     2041 ttcccggtgt tgatttccgc tatcgcatct tcaactgcga cggggacgag gtcgaacagt
     2101 gcggcaatgg cgcccgctgt ttcgccgtct ttgttcgtga agaggggctc accgacaaga
     2161 cgagcattcg tgttgagacg atgaaggccg tcatcgagcc cgaagtgagg cctgacggcc
     2221 gtgtgacggt gaacatgggg cctgcccgca aggcgcccga agtgttgccc tttgttcccg
     2281 aaggcctcga atccggcacg gaaggtgcct cgaggattta tcatgcgcat ctctcctgtt
     2341 ccgacgtctg gttttccgcc ttgtcgatgg gcaatccgca cgccgtgatc cgcgtcgagg
     2401 atgt
//
LOCUS       DBMDTI010000304         2404 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9686_length_2404_cov_3.291359, whole genome shotgun sequence.
ACCESSION   DBMDTI010000304 DBMDTI010000000
VERSION     DBMDTI010000304.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9686_length_2404_cov_3.291359"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            158..487
                     /locus_tag="ACZPB6_09920"
     CDS             158..487
                     /locus_tag="ACZPB6_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HIR1184818.1"
                     /translation="MTVDSSCGTAAEKERGEERSPDRWMVVFLNDDYTPFDWVIDVLI
                     RIFERTPVDAMTVTRSVHQQGRGIAGVYPRVKALRLVAKCMSEARRDGYPFTCIAVPA
                     EGGGLSL"
     gene            504..>2404
                     /locus_tag="ACZPB6_09925"
     CDS             504..>2404
                     /locus_tag="ACZPB6_09925"
                     /inference="COORDINATES: protein motif:HMM:NF036834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1184819.1"
                     /translation="MTEYRFQDAVEEIVRSTLFCAQEASDDWVPLLFLAFLCRHQATL
                     DALSQAGFDVERFEQANQAWLSKAEDVCRKVESCEAMTLADLPEFDAIEDAGVVSWLA
                     NAGIHVMSAGRRDRVVDVYDILIAIKTVMSAGSVISQAWDPAGVTLAELRRRDAVLHG
                     PQGFYTLTPPATTGFMADFLDDFLFGGPDEEARNEEVEEGEEGEGAATAGTESWGEVV
                     ADGRGGKVVRINLHDGDEVPEAFKKMIFRLADRVAGSDSGDEPENQAEAVETDKPRAK
                     GLSREEKYLQGCTRDLSAAAAGGELDPLIGRDAEVLRICEILCCRRKNKPLILGETGA
                     GKTALVEGLALRIHEGRVPAALRGLRIFALNASSMSSRYKGAFAEKLSEIVSVIKKVP
                     GAVLFIDNLHMLINGTDGNSQEEYNALGHLAGDDSLRLITTSTFRAWRQTFSKDDLLT
                     RRLQTVELAPVSRDEALRIVTGVLPKFEQYHGVHYAPEVAERAVALADRWITDRPFPD
                     KALDLVDELGGAARMNREAGNETPIEVGVQKLPELVARMARIPAEQISESEHGELAAL
                     DKVIRSAVFGQDEAIDRIVSAIRVSRSGLGNPERPVGSFLFTGPTGVGKTEVARRLAG
                     ALGVQLLRF"
BASE COUNT          441 a          696 c          815 g          452 t
ORIGIN      
        1 aaggcggaac gggaaagagg tctgccgcgc acactttgta cggacggtga cgttcaggcg
       61 gctttgcccc ctgaaagagc tgtctgacgg tcaaagtgaa tactctgtct tcagacaggg
      121 gagcttcaac ggatcacacg acactgggaa ggcagctatg actgtggatt cttcctgcgg
      181 cacggccgcg gaaaaggaaa ggggcgagga aaggtcgccc gatcgctgga tggtggtttt
      241 tctgaacgac gactacacgc cctttgactg ggtgatcgac gtactcatcc gaattttcga
      301 gcgcacgccg gtcgacgcca tgacggtcac gcgaagcgtg catcagcagg gcaggggcat
      361 cgcaggcgtc tatccgcgcg tcaaggccct tcgccttgtc gccaaatgca tgagcgaggc
      421 ccgcagagac ggctatccct tcacctgcat tgccgtgccc gccgagggcg gcggcctttc
      481 cctctgactg tccggagcta cttatgacgg aatatcgttt tcaggacgcc gtggaggaga
      541 tcgtcagatc cacgctcttc tgcgcgcagg aagccagcga cgactgggtt ccgcttcttt
      601 tccttgcctt cctgtgcagg catcaggcga cgctggacgc gctctcgcag gccgggtttg
      661 atgtcgagcg ctttgagcag gccaatcagg cgtggctctc caaggctgaa gacgtctgtc
      721 gcaaggtgga gtcgtgcgag gccatgacgc ttgcggacct gccggaattc gatgcaatcg
      781 aggatgccgg cgtcgtttcg tggcttgcca atgcaggcat ccacgtgatg agcgcggggc
      841 gccgcgatcg cgtcgtcgac gtctatgaca ttctcatcgc catcaagacg gtgatgtcgg
      901 cagggtccgt catttcccag gcttgggatc cggcgggggt tacgcttgcc gagcttcgca
      961 ggcgtgatgc cgtgcttcac ggtcctcagg gcttctatac gctgacgccg cctgcgacca
     1021 caggcttcat ggcggatttt ctcgatgact tcttgtttgg aggtcccgac gaggaggcca
     1081 ggaatgaaga ggtggaagag ggggaagaag gagagggcgc agcgacggcc gggacggagt
     1141 cttggggcga ggtcgttgcc gacggacgcg gcgggaaggt cgtgcgcatc aatctccatg
     1201 acggtgacga ggttccggag gcttttaaga agatgatctt ccgtctagcc gaccgggttg
     1261 ccgggagcga ttctggagac gagcctgaga atcaggctga ggcggtggag acggacaagc
     1321 cccgggcgaa gggcctgagc cgagaggaaa agtacctgca ggggtgcacg cgcgatttga
     1381 gcgcggcagc ggcaggaggg gagcttgatc ctctcatcgg acgcgacgcc gaggtgctgc
     1441 gcatctgcga aatcctctgt tgcaggcgca agaacaagcc gctgatcctc ggtgagacgg
     1501 gcgcgggcaa gacggcgctg gtcgaagggc ttgcgcttcg catccatgag ggccgcgtgc
     1561 ctgcggcact cagggggctc cgcatctttg cgctcaacgc ctcctccatg tcgagccgct
     1621 acaagggcgc attcgccgaa aagctttcgg aaatcgtctc cgtcatcaag aaggtgccgg
     1681 gagccgtgct cttcatcgac aacctgcata tgctgatcaa cggcaccgac ggcaactcgc
     1741 aggaggagta caacgcgctc ggtcatctgg cgggcgacga ttcgctgcgc ctcatcacga
     1801 cgtccacttt ccgcgcctgg cgccagacct tcagcaagga cgatctgctc acgcgtcgtc
     1861 tgcagacggt ggagctcgcg cctgtctcca gagatgaggc tctgcgcatc gtgacgggcg
     1921 tgcttccgaa gttcgagcag taccacggcg tgcattacgc gcccgaggtt gcagaaaggg
     1981 ccgtggcgct ggccgatcgc tggattacgg acaggccctt ccccgacaag gcgctcgatc
     2041 tggtggatga gcttggcggc gcagcccgca tgaacaggga ggcggggaat gagacgccca
     2101 tcgaggtcgg cgtgcagaag ctccccgagc tcgtcgcgcg catggcccgc attccggccg
     2161 agcagatttc cgagagcgag cacggtgagc tcgccgcgct cgacaaggtc attcgaagtg
     2221 ctgtgttcgg tcaggacgag gcgatcgaca gaatcgtttc ggcgatccgc gtctcccgtt
     2281 cggggctcgg caatcccgag cgtcccgtgg gctccttcct cttcacgggg ccgacgggcg
     2341 tcggcaagac cgaggtggcc cgcaggcttg cgggagcgct cggcgtacag cttcttcgtt
     2401 tcga
//
LOCUS       DBMDTI010000305         2400 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9702_length_2400_cov_2.704654, whole genome shotgun sequence.
ACCESSION   DBMDTI010000305 DBMDTI010000000
VERSION     DBMDTI010000305.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9702_length_2400_cov_2.704654"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="ACZPB6_09930"
     CDS             complement(<1..716)
                     /locus_tag="ACZPB6_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1184820.1"
                     /translation="MSLEVIHGGYAWPGREPVLTGVNFRFDGTGVMSILGPNGAGKTT
                     LLRCMLGLLKFTEGEARLDGRPVSNWKPRDFWRTIGYVPQAKLPGFASMTLADMVVLG
                     RSAHIGPFSLPSDADWEVVDRVMTEVGIEHLAGRLCSEVSGGQFQLALIARALAAEPR
                     ILVLDEPESNLDFRNQMVVLNVIERLTEQGLGAVINTHFPAHALEISTKTLLVPRHRP
                     PIFGDTRDVMTEDLLSDVFD"
     gene            complement(721..1779)
                     /locus_tag="ACZPB6_09935"
     CDS             complement(721..1779)
                     /locus_tag="ACZPB6_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR1184821.1"
                     /translation="MSNVSTARARSAQAAGAAAGRFRLVMASLALLLAVTAVASLCLG
                     RYSLTGTEVMAVLLPDGWLSVETNRMMENIVLNVRLPRVLLAIIAGAGLAMSGAAFQA
                     LFANPLAAPDTLGVATGASFGAVLGILWGWNGMGIQLMSLATGLSAVFFVIVISRVRN
                     TSSILMIILSGMVVGALFSALVSLVKYAADPQDVLPSITFWMMGALTGATKSSVLAGT
                     PMVLLGMLVLWLLRWRLNVTTLPTDEAASLGIPVRRIRAGVIIAATMITASVVSMCGL
                     IGWVGLLVPHAARMVFGANNRFVLPASMVMGALFMLVIDTVARTATQSEIPVSILTAV
                     VGAPFFIYLLRRTGGISG"
     gene            complement(1783..>2400)
                     /locus_tag="ACZPB6_09940"
     CDS             complement(1783..>2400)
                     /locus_tag="ACZPB6_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1184822.1"
                     /translation="GKGDLIDSRSKAIAKMVADRTKDLRPEERSKVLFLFRYDARQIV
                     TSGRNFFGQYWCDAVGARNVAESVTADNANAIVSMEQIYAWNPDVVFITNFTAATPAD
                     LFDNKMAGHDWSDVKAVKDKRVYKLPLGIYRSYTPSADTPLTLLWIAKQVYPSRFADI
                     DLTKEVKAWYKDVFGVTLTDADVDMMFNPGEGASTGATVATRSNG"
BASE COUNT          426 a          711 c          887 g          376 t
ORIGIN      
        1 ctgtcaaaga cgtctgagag gagatcctcg gtcatgacgt cgcgggtgtc gccgaagatg
       61 ggcggcctgt gcctggggac gaggagcgtc ttcgtggaga tctcgagcgc atgcgcgggg
      121 aagtgcgtgt tgatgacggc gccgagaccc tgctcggtga gacgctcgat gacgttgagg
      181 accaccatct ggttgcggaa gtcgaggttc gattcaggct cgtcgagcac gaggattctg
      241 ggttcggccg cgagcgcgcg ggcgatgagc gcgagctgga actgcccgcc cgagacttcg
      301 ctgcagaggc gccccgcgag gtgctcgatg ccgacctcgg tcatgacgcg gtcgaccacc
      361 tcccagtccg cgtcggatgg caggctgaag gggccgatgt gcgcgctgcg gccgaggacg
      421 accatgtcgg cgagcgtcat cgaggcgaag cccgggagct tcgcttgagg tacgtagccg
      481 atcgtgcgcc agaagtcgcg gggcttccag ttggagacgg ggcggccgtc gagcctcgcc
      541 tcgccctccg tgaacttgag aaggccgagc atgcagcgca gcagcgtcgt cttgcccgcg
      601 ccattggggc cgaggatgct catgacgccc gtgccgtcga agcggaagtt gacgcccgtg
      661 agaacgggtt cgcggcccgg ccaggcgtag ccgccgtgga tcacttcaag ggacatggcg
      721 ctaccccgag atgccgcccg tgcggcgcag aagatagatg aagaacggcg cacccacgac
      781 ggccgtgagg atcgagacgg gaatctcgct ctgcgtggcg gtgcgcgcga cggtgtcgat
      841 cacgagcatg aagagggcgc ccatgaccat cgaggcgggc agcacgaagc ggttgttcgc
      901 accgaagacc atgcgtgcgg cgtgggggac gagaaggccc acccagccga tgaggccgca
      961 catcgagacg accgaggccg tgatcatggt ggcggcgatg atgacgcctg cgcggatcct
     1021 gcgcacgggt atgccgaggc ttgcggcctc gtcagtcgga agggtcgtca cgttgaggcg
     1081 ccagcgcagg agccagagaa cgagcatgcc gaggagcacc atgggcgtgc ccgcgagcac
     1141 gcttgacttg gtcgcgcccg tgagcgcccc catcatccag aaggtgatgg acggcagcac
     1201 gtcctgcggg tcggccgcgt acttgacgag ggagacgaga gccgagaaga gggctccgac
     1261 gaccatgccc gagagaatga tcatgaggat cgagctcgtg ttgcgcacgc ggctgatgac
     1321 gatcacgaag aagactgcgg agaggcccgt cgccaggctc atgagctgaa tgcccatgcc
     1381 gttccagccc cagaggatgc ctaggacggc gccgaacgag gcgcccgtgg cgacgccgag
     1441 cgtgtcgggc gcggcgagcg gattcgcaaa gagcgcctga aaggcggcgc ccgacatggc
     1501 gagtcccgcg cccgcaatga tcgcgagaag cacgcgcggc agcctcacgt tgagaacgat
     1561 gttctccatc atgcggttcg tctcgacgga aagccagccg tcgggcagga gcacggccat
     1621 cacttcggtg cccgtgagac tgtagcggcc gaggcacagc gaggcgacgg ccgtgacggc
     1681 gagcagcagg gcgagcgagg ccatgacgag gcggaagcgc cccgcggccg cgcctgcggc
     1741 ctgagcgctt cgcgcgcgtg cggtggagac gttcgacatg gatcagccgt tgctgcgggt
     1801 ggcgaccgtg gcgccggtgc tcgcgccttc gcccggattg aacatcatgt cgacgtcggc
     1861 gtcggtgagc gtcacgccga agacgtcctt gtaccaggcc ttgacctcct tggtgagatc
     1921 gatgtcggca aagcgcgagg gatagacctg cttggcaatc cagagcagcg tgagcggcgt
     1981 gtcggccgaa ggcgtgtagc tgcggtagat gccgagcggg agcttgtaga cgcgcttgtc
     2041 cttgacggcc ttcacgtcgg accagtcgtg gcctgccatc ttgttatcaa agaggtcggc
     2101 gggcgtggcg gccgtgaagt tcgtgatgaa gacgacgtcg gggttccagg cgtagatctg
     2161 ctccatcgag acgatggcgt tggcgttgtc tgccgtcacg gactcggcga cgttcctggc
     2221 gccgacggcg tcgcaccagt actggccgaa gaagttgcgc ccagaagtga cgatctggcg
     2281 ggcgtcgtag cggaagagga agagaacctt gctgcgctcc tcgggcctga ggtccttcgt
     2341 gcggtcggcg accatcttcg cgatggcctt cgagcggctg tcgatcaggt cgcccttgcc
//
LOCUS       DBMDTI010000306         2398 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9714_length_2398_cov_3.151937, whole genome shotgun sequence.
ACCESSION   DBMDTI010000306 DBMDTI010000000
VERSION     DBMDTI010000306.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9714_length_2398_cov_3.151937"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /gene="rsgA"
                     /locus_tag="ACZPB6_09945"
     CDS             <1..1008
                     /gene="rsgA"
                     /locus_tag="ACZPB6_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR1184823.1"
                     /translation="SPATFLRGSRPLTNLSYCPMTKTPSRKHRPTAEAAAPLITGRVT
                     AGHGRHYFVTTEDGAVLEAHRRGKKGDVVVGDVVECTEPVSGVSAIESIRERRNLLYR
                     SDEWRIKSLAANIDLVCIVFASRPTFNPWFIWRALLAAHQAGIPALVIRNKAELAEGA
                     EEAAAAVRLLESIGHDVITVSATGEPEATRARLIERLEGRASLLVGQSGMGKSTILNL
                     LVPHAQAATREFSVALNLGKQTTTAARWYDAKYDDWQGSVIDTPGFQEFGLAHLSLND
                     ILRAMPDIAAHVSGCRFFNCRHLEEPGCGVKAAVEAGEVDEARYAFYRSLAVHADTLP
                     L"
     gene            1065..2171
                     /gene="moaA"
                     /locus_tag="ACZPB6_09950"
     CDS             1065..2171
                     /gene="moaA"
                     /locus_tag="ACZPB6_09950"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR1184824.1"
                     /translation="MAEVIPIATNPAPVRSLPIPQTGAVRRSVGHWTDPRGRPLRDLR
                     ISVTDHCNFRCRYCMPKEKFSNPHAFLAHTELLTFEEILRIARIVVANGVEKLRLTGG
                     EPLLRKGLEELVAELRRLRTPDGRDIDIALTTNASILHKKAQALRDAGLDRVTISLDA
                     LDEGIFQGFNDVGFPAAKVLDNIRLAADMGFRVKINTVVKRGVNESEIVRICEHFRHT
                     GIIPRFIEYMDVGTSNGWRMTDVVPSAEVVGMIRAKWPVEPVGANYPGETASRWRYLD
                     GAGEFGCISSVTQAFCGECSRARLSMDGRLYLCLFATEGYDIRTMLRGGATDFEIEQA
                     LGRIWSARDNHYSELRSLGLAPERAKIEMSYIGG"
BASE COUNT          424 a          917 c          658 g          399 t
ORIGIN      
        1 tcaccagcaa cctttcttcg cggctcacgt ccgctcacga acctcagcta ctgtcccatg
       61 accaaaactc cgtcccgcaa gcaccgtccg accgccgaag cagccgcccc cctcatcaca
      121 ggccgcgtca ccgcaggcca cggccgccac tacttcgtga cgacggagga cggcgccgtg
      181 cttgaagccc accgccgcgg caagaagggc gacgtcgttg tgggcgacgt cgtcgagtgc
      241 accgaaccgg tctcgggcgt ctccgccatc gagtcgatcc gagagcgccg aaaccttctc
      301 taccgctccg acgaatggcg catcaagtcg ctcgccgcca acatcgacct cgtctgcatc
      361 gtcttcgcgt cccgcccgac cttcaacccc tggttcatct ggagggcgct tctcgccgcc
      421 catcaggcag gcatccccgc gctcgtcatc cgcaacaagg ccgaacttgc cgaaggcgcc
      481 gaggaggctg cggccgccgt acgccttctc gagtcgatcg gccacgacgt catcacggtc
      541 tccgccacgg gcgagcccga ggcgacccgc gcccgcctca tcgagcgtct cgaaggccga
      601 gcctcgctcc tcgtcggcca gtcgggcatg ggcaagtcca cgatcctcaa ccttctcgtc
      661 ccgcacgccc aggccgccac gcgcgaattc tccgtggccc tcaacctcgg caagcagacg
      721 accaccgccg cccgctggta cgacgccaag tacgacgact ggcaaggcag cgtcatcgac
      781 accccgggct tccaggagtt cggcctcgca cacctctcgc tcaacgacat cctgcgcgcc
      841 atgcccgaca tcgcagcgca cgtctcgggc tgccgtttct tcaactgccg gcacctcgag
      901 gagcccggct gcggcgtcaa ggccgccgtc gaggcgggcg aggtcgacga ggcgcgctac
      961 gccttctaca ggtcgctcgc agtccacgcc gacacgctgc ctctataatc attcaatttc
     1021 cccgaaacga ctcccgcaga aacgttccac agaggtccac cgtcatggcc gaggtcattc
     1081 ccatcgctac gaatcccgcc cccgtgcgca gtcttccgat tccgcagaca ggcgccgtgc
     1141 gacgctcggt aggccactgg accgaccccc gcggacgccc cctgagggac cttcgcatct
     1201 cggtgaccga ccactgcaac ttccgctgcc gctactgcat gcccaaggag aagttcagca
     1261 atccccacgc ctttctcgcc cacaccgagc tcctcacctt cgaggaaatc ctgcgcatcg
     1321 cgcgcatcgt ggtcgccaac ggcgtcgaga agttgcgcct cacgggcggc gagcccctgc
     1381 tcagaaaggg cctcgaggaa cttgtcgcag aactgcgcag gctcaggacg cccgacggcc
     1441 gcgacatcga catcgcgctc acgacgaacg cctccatcct tcacaagaag gcgcaggccc
     1501 tgcgcgacgc gggcctcgac cgcgtcacga tcagcctcga cgcgctcgac gaaggcatct
     1561 tccagggctt caacgacgtg ggctttcccg ccgccaaagt gctcgacaac atcaggcttg
     1621 cggccgacat gggctttcgc gtcaagatca acaccgtcgt caagcgcgga gtgaacgagt
     1681 ccgaaatcgt ccgcatctgc gaacacttcc gccacacggg catcatcccc cgcttcatcg
     1741 agtacatgga cgtgggcacc tccaacggct ggcgcatgac cgacgtcgtt ccctccgcgg
     1801 aggtggtcgg gatgatccgc gcaaagtggc ccgtcgagcc cgtcggcgcc aactatccgg
     1861 gtgaaaccgc ctcccgctgg cgctatctgg acggcgctgg cgaattcggc tgcatctcct
     1921 ccgtcaccca ggccttctgc ggcgaatgct cccgcgcgcg cctgagcatg gacgggcgcc
     1981 tctacctctg cctcttcgcg accgagggct acgacatccg cacgatgctc cgcggcggcg
     2041 ccaccgactt cgagatcgag caagcgctcg gccgaatctg gtccgcccgc gacaaccact
     2101 actccgagct ccgttcgctc ggtctcgcgc ccgagcgcgc gaagatcgaa atgagctaca
     2161 tcggcgggta gtttcgccta ggcatcttgc cttagtcctt caccttttct cccgttcagt
     2221 gggacagaag gcgttcgcgt ccgtatactt gaatacccga acacaagtct tcggtcatcc
     2281 ggcggccagg ccgcgctttt tttttgcgcc cgttttcttc accctgccag gcccccttct
     2341 cgatgacgcg cccgccgcgc ccccgcccgg cgaacaatca atataagaaa gggtcccc
//
LOCUS       DBMDTI010000307         2393 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9734_length_2393_cov_2.085515, whole genome shotgun sequence.
ACCESSION   DBMDTI010000307 DBMDTI010000000
VERSION     DBMDTI010000307.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9734_length_2393_cov_2.085515"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="ACZPB6_09955"
     CDS             complement(<1..295)
                     /locus_tag="ACZPB6_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1184825.1"
                     /translation="MALNVVEIWRDLHKTPELGFEENKTSAYLAEALEKLGFAVTRGV
                     GKTGVMGVIRGAEPGPVLMLRADMDALPFRNDDGSIEAVHACGHDSHCAMVLAA"
     gene            534..2024
                     /locus_tag="ACZPB6_09960"
     CDS             534..2024
                     /locus_tag="ACZPB6_09960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008543864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184826.1"
                     /translation="MQAARRNLLKTGLMCTGAAAAAGVSLPASAAVEPKSKIAREEWD
                     VVVVGAGFAGLCAALEAKEKGAKVLLIEKMGRPDCTSAYSSGWIAATKTRYQDKDDDD
                     SKELYFKEMMEVSGGRSDPALIRAYAEIAGESVDWLGDHGMTYKIWKQHAPERMRCHI
                     VPAVNGLTGGAHMLKVMLEALEKAGVPVRYNTKAVELVTDECFNVLGVSCIEKHRRVE
                     LMTKGGVILATGGFAGNNAMVGQYIGPWASRMVVRGAPWATGENIRMAEQVMARMVNM
                     DQFYAGPISPVGHCNPSPLMHAGYGIQINTDGRRFVQEHLGQIEKAVGIASLTKNNMS
                     YLLIGQDADANNNILSNTLTRFEKLGLKVASGKTIEEAVKQAGLPVEAVMETVAEYNK
                     AVREGKTAELVPPYLNEHPHELKTGPFYLVPAVGGIANTFGGPKIDANARVVNTEDRP
                     IPGLYAAGAAAGGIWYAADVSGSQLGGCMVFGRLAARHAAARAKKA"
     gene            2051..>2393
                     /locus_tag="ACZPB6_09965"
     CDS             2051..>2393
                     /locus_tag="ACZPB6_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR1184827.1"
                     /translation="MKKLALAGALAMIAAAATAAQPGHLADRHAARSVDCAACHAVKT
                     PAEGAEVDTQKCLACHVSLNAVSEKIHARGNAQSPDPHVNHSLGLNCVECHKGHEQSV
                     NMCSRCHLFEYK"
BASE COUNT          455 a          779 c          775 g          384 t
ORIGIN      
        1 cggcggcgag caccatcgcg cagtggctgt cgtggccgca ggcgtggacg gcctcgatcg
       61 agccgtcatc gttcctgaag ggcagggcgt ccatgtcggc gcgaagcatc aggacggggc
      121 cgggctcggc gcccctgatg acgcccatga cgcccgtctt cccgacgccg cgcgtgacgg
      181 cgaagccgag cttttcgagg gcctcggcaa ggtaggcgga ggtcttgttt tcttcaaaac
      241 cgagttcggg cgtcttgtgc aggtcgcgcc agatctcgac gacgttgagt gccatgctgg
      301 tctccaatgt gaagcggatg ggttggttgg atgaattctc aatattaccg cgcccggcgc
      361 agggcagcag gcctgaggtt ttccttcagg ggcgcccggg acgccgcccg atggccgaaa
      421 aagcgcatcg aatgtccttc ctgaggttag tgcgccgaag tgttccgtcg tagcatgaaa
      481 aggacgtcgg acgtgaccgg tccggcgccg ctcaagacaa aaggagagaa atcatgcaag
      541 ctgcacgccg caatctgctc aagaccggcc tcatgtgcac gggtgccgcc gctgccgcag
      601 gcgtctcgct cccggcctcc gccgcagtcg agcccaagtc gaagatcgcc cgcgaggagt
      661 gggacgtcgt cgtggtgggc gcgggcttcg cgggcctctg cgccgcgctc gaagccaagg
      721 aaaagggcgc caaggttctt ctcatcgaga agatgggccg tcccgactgc acgtccgcct
      781 acagctccgg ctggatcgcc gcgacgaaaa cgcgctatca ggacaaggac gacgacgatt
      841 ccaaggaact ctacttcaag gagatgatgg aggtttcggg cggtcgttcc gatcctgccc
      901 tcatccgcgc ctatgccgag attgcgggcg aaagcgtcga ctggctcggc gaccacggca
      961 tgacgtacaa gatctggaag cagcacgcgc ccgaacgcat gcgctgccac atcgtgcctg
     1021 cagtcaacgg cctcacgggc ggcgcccaca tgctgaaggt gatgctcgag gcgcttgaga
     1081 aggcgggcgt gcccgttcgc tacaacacga aggcggtcga gctcgtgacc gacgagtgct
     1141 tcaacgtgct cggcgtctcc tgcattgaaa agcaccgccg cgtcgaactg atgaccaaag
     1201 gcggcgtgat tctcgccacc ggcggctttg ccggcaacaa cgccatggtc ggccagtaca
     1261 tcggcccgtg ggcctcccgc atggttgtgc gcggcgcgcc ctgggcgacc ggtgaaaaca
     1321 ttcgcatggc cgaacaggtc atggccagga tggtgaacat ggaccagttc tatgcgggtc
     1381 cgatttcgcc cgtgggtcac tgcaaccctt cgccgctcat gcatgcgggc tacggcatcc
     1441 agatcaacac ggacggcagg cgcttcgtgc aggagcacct cggccagatc gagaaggccg
     1501 tgggcatcgc cagcctgacg aagaacaaca tgagttacct gctcatcggt caggacgccg
     1561 acgcgaacaa caacatcctc tcgaacacgc tcacgcgctt cgagaagctc ggcctcaagg
     1621 tggcctcggg caagacgatc gaggaggccg tcaagcaggc gggcctgccg gtcgaggccg
     1681 tcatggagac ggttgccgag tacaacaagg ccgtgcgcga agggaagacc gccgagctgg
     1741 tgccgccgta cctcaatgag catccgcatg agctcaagac cggtcccttc tatctcgttc
     1801 ccgccgtggg cggcatcgcc aacaccttcg gcggcccgaa gatcgacgcc aacgcgcgcg
     1861 tcgtcaacac cgaggatcgt ccgattccgg ggctctacgc tgcgggcgcc gcagcgggcg
     1921 gcatctggta tgcggccgac gtctcgggca gccagctcgg cggctgcatg gtgttcggac
     1981 gccttgccgc gcgccacgcc gcggctcgtg cgaagaaggc ttgatcggga ggaaaggaaa
     2041 tatgttcgac atgaagaaac tggcgcttgc cggcgccctt gcaatgatcg ccgcggccgc
     2101 cacggccgcc cagcctggcc acctcgccga ccgccatgcc gcgcgctcgg tcgactgcgc
     2161 cgcctgccat gcagtgaaga ctccggccga aggcgccgaa gtcgacacgc agaagtgcct
     2221 tgcctgccac gtctcgctca acgccgtctc cgaaaagatc cacgcccgcg gcaacgccca
     2281 gtcgccagac ccgcacgtga accacagcct cggcctcaac tgcgtcgagt gccacaaggg
     2341 ccacgaacag tcggtcaaca tgtgcagccg ctgccatctc ttcgagtaca agg
//
LOCUS       DBMDTI010000308         2390 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9757_length_2390_cov_1.765400, whole genome shotgun sequence.
ACCESSION   DBMDTI010000308 DBMDTI010000000
VERSION     DBMDTI010000308.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9757_length_2390_cov_1.765400"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="ACZPB6_09970"
     CDS             <1..714
                     /locus_tag="ACZPB6_09970"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1184828.1"
                     /translation="ICDGAMKRLHEALQREKAFTGDVSHELRTPLTVIETSAELLSMT
                     PLSPAQAKQVERITRSAGDMRELMSLFLSFARLSNETTGPEPDSAAGILKAVADTWEP
                     FAAEKGLALVLRREAECPGAYSPVMLGTVANNLVKNAVAYTESGVVTITETSEGFIVS
                     DSGPGLAGDEAARIFEHGVRGSAAVADGSGAGLGLSIVSRICRRSGWTVTAGKAPEGG
                     AAFTVTMTKAVDARRADRL"
     gene            complement(747..1472)
                     /gene="yddG"
                     /locus_tag="ACZPB6_09975"
     CDS             complement(747..1472)
                     /gene="yddG"
                     /locus_tag="ACZPB6_09975"
                     /inference="COORDINATES: protein motif:HMM:NF008676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid DMT transporter YddG"
                     /protein_id="HIR1184829.1"
                     /translation="MGAANVCAICFIFSLSMAEDATQTMQVGMVNYLWPCLTIVFAVL
                     FNGERARWWIVPGVLLSLLGVMVVLGGEDGFNPMHFAANWMKNPWSYTIALVGAVLWA
                     GYGSMTRAWSGGHNPVVIIFFNDMLIFGLIWLLTPTPPGEWMHPGWLGVFLGAAATGL
                     GYGVWTYGMQYGNMTLLGIGSYFTPVLSCVFASFYIGSDLSATFWQGVALVVLGSLVC
                     WTSVRKTKPVERKGEPLNLHHKH"
     gene            complement(1485..1679)
                     /locus_tag="ACZPB6_09980"
     CDS             complement(1485..1679)
                     /locus_tag="ACZPB6_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184830.1"
                     /translation="MDASTRATAVGLLAPVLWGMSVGICRGIAEDFGRGPGFTVMYAA
                     ACLLLLLIFGRPHLRAFSLK"
     gene            complement(1767..>2390)
                     /locus_tag="ACZPB6_09985"
     CDS             complement(1767..>2390)
                     /locus_tag="ACZPB6_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1184831.1"
                     /translation="IVADVRVAGMLTGKTGFVHVHLGDYTSSFDIFDGIVASGLPIKH
                     IRPTHVARHPAVFERAMGFAKQGGWIDITTGGGNYMGCAADAYDMAVENGVPVNRITM
                     SSDGHGSMPRFNEAGEMVGLGVGSIMCNIETVQELARRHDLTTALLPMTRTVAEALTL
                     EGKGVIEVGADADLLILNAEHEITDVFMGGVQCMRAGEVIVKGAFEE"
BASE COUNT          449 a          776 c          785 g          380 t
ORIGIN      
        1 atctgcgacg gcgccatgaa gaggctccac gaggccctgc agcgcgagaa ggccttcacg
       61 ggcgacgtga gccacgaact caggacgccg ctcaccgtga tcgagacgag cgccgagctt
      121 ctctcaatga cgccgctctc gcccgctcag gcgaagcagg tcgagcgcat cacgcgctct
      181 gcgggcgaca tgcgcgagct gatgtcgctc tttctctcct tcgcccgcct ctcgaacgag
      241 acgacgggcc ccgagcccga ctccgccgcg ggcattctca aggccgtcgc cgacacatgg
      301 gagccgtttg cggccgaaaa gggcctcgcg ctcgtgcttc gccgcgaggc cgagtgcccc
      361 ggcgcctact cgcccgtcat gctcggcacc gtcgccaaca acctcgtgaa gaacgccgtc
      421 gcctacaccg aaagcggcgt ggtcacgatc acggaaacgt ccgagggctt cattgtctcc
      481 gactcgggac cgggactcgc cggcgacgag gccgcccgca tcttcgagca cggcgtgcgc
      541 ggcagtgcag ccgtcgccga cggctcgggc gccggactcg gcctttcgat cgtctcgcgc
      601 atctgccgcc gctcgggctg gacggtaacg gcgggcaagg cccctgaagg cggcgccgcc
      661 ttcacggtca caatgacgaa ggcggtcgac gcacgacgcg ccgaccgcct ttgagaaaac
      721 gtctttgaga aaagactcaa ctggtctcag tgcttgtgat gcagattgag gggctcgccc
      781 ttgcgttcga caggcttcgt ctttcgcacc gacgtccagc acacgagtga accgagcacg
      841 acgagcgcga cgccctgcca gaacgtggcg ctcagatccg acccgatgta gaaggaggcg
      901 aagacgcagg agaggacggg cgtaaagtag gagccgattc ccagaagcgt catgttgccg
      961 tactgcatgc cgtaggtcca gacgccgtag cccagtcccg tcgcggccgc gccgaggaag
     1021 acgccgagcc aaccgggatg catccactcg ccgggcggcg tgggcgtaag cagccagatg
     1081 agcccgaaga tcagcatgtc gttgaagaag atgatgacca cgggattgtg cccgcccgac
     1141 caggccctgg tcatcgagcc gtagcccgcc cagagcacgg cgcccacgag ggcgatcgtg
     1201 tagctccacg gattcttcat ccagttggcc gcaaagtgca tcggattgaa tccgtcctcg
     1261 ccgccgagca ccaccatgac gcccagcagg ctcagcagca cgcccggcac gatccaccac
     1321 ctcgcccgct cgccgttgaa gagcaccgcg aagacgatcg tcaggcaggg ccacagatag
     1381 ttaaccatgc cgacctgcat ggtctgcgtg gcgtcctcgg ccatcgatag cgagaagatg
     1441 aagcagatgg cgcagacgtt tgcggcgccc acgcccagaa gcagttactt gagggagaag
     1501 gcccgcaaat gcgggcgtcc gaagatgaga agaagcagaa gacaggccgc ggcgtacatc
     1561 accgtgaagc ccgggcctcg cccgaagtcc tcggcgatgc cgcggcagat cccgacgctc
     1621 atgccccaga ggacgggtgc gagaagaccg actgcagttg cgcgtgttga tgcgtccatt
     1681 tgcgaaaaag gcccgccgct tgaatgttcg cggcggacct gagtgaggtc acttgcggtt
     1741 gttgctcagg acattatgcc cgatgttcat tcctcaaagg cgcccttgac gatcacttcg
     1801 ccggcgcgca tgcactggac gccgcccatg aagacgtcgg tgatctcgtg ttcggcgttg
     1861 aggatgagca ggtcggcgtc ggcgccgacc tcgatgacgc ccttgccttc gagcgtgagc
     1921 gcttcggcga cggtgcgggt catcggcagc agtgcggtcg tcaggtcgtg gcggcgcgca
     1981 agttcctgaa cggtctcgat gttgcacatg atggagccga cgccgaggcc gaccatttcg
     2041 cccgcctcgt tgaagcgcgg catggagccg tggccgtcgg agctcatcgt gatgcggttg
     2101 acgggcacgc cgttttcgac ggccatgtca taggcgtcgg ccgcgcagcc catgtagttg
     2161 ccgccgcccg tggtgatgtc gatccagccg ccctgcttcg cgaaacccat cgcgcgttcg
     2221 aagacggcgg gatgacgggc gacgtgcgtc ggacggatgt gcttgatcgg caggccggag
     2281 gcgacgatgc cgtcaaagat gtcgaaggac gacgtgtagt cgccgagatg aacgtgcacg
     2341 aagccagtct tgcccgtgag catgcccgcg acgcggacgt cggcgacgat
//
LOCUS       DBMDTI010000309         2369 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9874_length_2369_cov_2.250000, whole genome shotgun sequence.
ACCESSION   DBMDTI010000309 DBMDTI010000000
VERSION     DBMDTI010000309.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9874_length_2369_cov_2.250000"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="ACZPB6_09990"
     CDS             <1..655
                     /locus_tag="ACZPB6_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HIR1184832.1"
                     /translation="DSSIRFGAALSYTGSEADYRRGNADMDLYGLSAYGLWMGEAGQF
                     VDVVGRLASAKTDMTVDGGKKGSMDNVALSLSGEVGWRFDVSSFFYVEPQVEATYTFV
                     NADRLELSDGSNYEFDDAHSLLGRAGMAFGVKCPNEMGSVHARVSAVHEFLGDLKVTG
                     GNGAILETDGKDTWVEYGLGVNFNLTPATYFWADVERTSGGVLDEDWRATVGVRHAF"
     gene            899..1387
                     /locus_tag="ACZPB6_09995"
     CDS             899..1387
                     /locus_tag="ACZPB6_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HIR1184833.1"
                     /translation="MIASAPFQEVLSSALTPITLISGVGMLILCMTNRYSHCMDRIRG
                     LVKIRTQGLKALRVEPDIDREIRLIYRRARWLRFSMLAITLSSGCSGLLVATNVFGTY
                     FGFDAAPFAAVWLVLALLLIIAATVAFAFEIRLSLHALGLAVEHMPRGDALKKRLEEA
                     SK"
     gene            1384..1974
                     /locus_tag="ACZPB6_10000"
     CDS             1384..1974
                     /locus_tag="ACZPB6_10000"
                     /inference="COORDINATES: protein motif:HMM:NF022479.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HIR1184834.1"
                     /translation="MTSFNELLASALAPITLISGVGLLMLSMSARYNHATSRIRLLMN
                     ERLDPKLTDDERADIDDEIDFIFHRAGLLRRGEFCVVLSAVVSGLLVALCVLEEATGF
                     SADILNSALLLASVALIVLSTVFLSLEIRLSLRAIKLVVSKLPPVADAFDDAVAARAA
                     QAVEARHQSHVLNEARRQAEHAASHEVRKAEGGHSA"
BASE COUNT          413 a          699 c          726 g          531 t
ORIGIN      
        1 cgacagctcg atccgcttcg gcgcagcgct gagctatacg ggcagcgaag ccgactacag
       61 gcgcggcaat gccgacatgg acctctatgg tctttcggcc tacggcctgt ggatgggtga
      121 ggccggccag ttcgtggacg tggtgggtcg tcttgcctcc gccaagaccg acatgacggt
      181 tgacggcggc aagaagggtt cgatggacaa cgtggcgctg agcctctcgg gcgaggtcgg
      241 ttggcgcttc gacgtctcga gcttcttcta cgtcgagcct caggtcgagg ccacgtacac
      301 gtttgtcaat gccgatcgcc ttgagctcag cgacggctcg aactatgagt ttgacgatgc
      361 gcacagcctg ctcggccgtg caggcatggc cttcggcgtg aagtgcccga acgaaatggg
      421 cagcgtccat gcgcgcgttt cggccgtcca tgagttcttg ggcgacctga aggtgacggg
      481 cggcaacggt gcgatccttg agacggacgg caaggacacc tgggtcgaat acggcctcgg
      541 cgtcaacttc aacctgacgc ccgccacgta cttctgggcc gacgtcgagc gcacctcggg
      601 cggcgtcctg gacgaggact ggcgcgcgac ggtgggcgtg aggcacgcct tctgaccttt
      661 cattgacttc tgaagcgcag gccgcccggg aattcttctc ccgtgcggcc tatttctcgt
      721 gctgcggacg gacgcgcgtg gtgagtggac gaaatctgcc tttcgtgatg ttggcgacct
      781 gtggtaaatc cgtgctgcgg cttgattttg gacaacgctt ttcttcgcgc cgtcacccgc
      841 cggaactcaa gcggtctata gtcgaaaacc cgcaattgcc gatcaagacg gaggtgcgat
      901 gatcgcctcg gctccctttc aagaagtgct ttcaagcgca ctcacgccca tcacgctcat
      961 atcgggcgtg ggcatgctca ttctctgcat gacgaaccgc tacagccact gcatggatcg
     1021 cattcgcggc ttggtgaaga ttcgcacgca gggcctcaag gcgcttcgcg tcgagcccga
     1081 catcgaccgc gagattcgtc ttatctatcg acgggcgcgc tggttgcgct tttcaatgct
     1141 cgccatcacg ctctcgagcg gatgctccgg gttgcttgtg gcgacgaacg tcttcggcac
     1201 ctacttcggc ttcgacgcgg ctccttttgc cgccgtctgg cttgtgctgg cgcttttgct
     1261 catcattgcc gccaccgtcg ccttcgcctt cgagatcagg ctttcgctcc atgcgctcgg
     1321 ccttgccgtc gagcacatgc ccagaggcga cgctctcaaa aagcgcctcg aggaggcatc
     1381 gaaatgacgt ccttcaacga gcttctcgct tccgcgctcg cccccatcac gctcatctcg
     1441 ggcgtcggcc ttctgatgct atccatgagc gcccgctaca accacgcgac gagcaggatt
     1501 cggctcttga tgaacgagcg gctcgacccg aagctcaccg acgacgaacg cgcggacatc
     1561 gacgatgaaa tcgacttcat cttccaccgg gcggggcttt tgcgccgcgg tgaattctgc
     1621 gtcgttcttt ccgccgtcgt gtcgggactt ctcgttgcgc tctgtgtcct cgaagaggcg
     1681 acgggtttca gcgccgacat tctcaacagt gcgctgctgc tcgcttcggt ggctctgatc
     1741 gtcctgtcga ccgtgttcct gagccttgag attcgtctct ccttgagagc catcaagctg
     1801 gtcgtctcaa agctgccgcc ggttgcggac gccttcgatg atgcggttgc agcaagagcc
     1861 gctcaggctg tcgaggcgcg tcatcaatcg cacgttctca atgaagcgag gcggcaggcc
     1921 gaacatgcgg cgtctcatga ggtgagaaag gccgagggcg ggcactccgc ctgagaagtt
     1981 ggcaaggcct tcttttgcag tgcatcttgg gtgcaagtca ggcttggata tgcgtacgga
     2041 tgatcgaaaa tgccgatgca tccgcacgcg gcggtcatct gcttctgcct gtcggttaaa
     2101 aggtttttat aacggctgta ctcgtgtacg gcctttattt gaaactgttt gtaatcctat
     2161 ttgctgggac gattaatatg ccttgattgt ccaatccgtc aaccggagaa gagggcgtac
     2221 gggcgttttg cctctttcgc ggagatgagg atgggagaaa tgctcatgac gctcatgggc
     2281 gacggtccta tactcaaacg agtgcccgag tcattcgggc gcgtttagaa tgggaggtcc
     2341 cgagggaaag ggcgcacggc tgcattgga
//
LOCUS       DBMDTI010000310         2349 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_9986_length_2349_cov_2.199733, whole genome shotgun sequence.
ACCESSION   DBMDTI010000310 DBMDTI010000000
VERSION     DBMDTI010000310.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9986_length_2349_cov_2.199733"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="ACZPB6_10005"
     CDS             complement(<1..1013)
                     /locus_tag="ACZPB6_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184835.1"
                     /translation="MNAILAGLQQFSPDRIMTAAGFLLVLLVALAMWLAARRRQARRN
                     IGLSKIAAGLPEGDGPVTRAAPKLSRPDPLEADRLALEMLESDENDAEDIEEIRHTSY
                     TERVNVPNAAARREAAPEPRREPDVAVLAPEPKPEPDPAPRPEPAFRREPEPAPVPAV
                     PVVPPMPEWEFLKTLPFIVRQGLSTGHADMLGIGVEAVFFPAEAVNRLDDPVLRLVPF
                     LPADEGELLCRESDRRTFQAGGLYAQPDAVIALRAGGVLAVEYKSRGGRMDDPHDIPG
                     SLRPKDLLQTVIGAMVLSASEGVACAPVLRTNNAVYFLRPGKTLATLLAERIGAAVSF
                     VKP"
     gene            complement(1016..1849)
                     /locus_tag="ACZPB6_10010"
     CDS             complement(1016..1849)
                     /locus_tag="ACZPB6_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR1184836.1"
                     /translation="MAGTIYCLPNLIADGTLEAAIPPAVRTRAADIRLFFVEAAKNAR
                     AYLKLLGHPGPISELRIEEIGHDPDPALIDRWLEPVLAGEDAAIVSESGCPGIADPGA
                     QIVARAQELGLRVVPWVGPSSILMTLMASGLDGQRFRFLGYLPVHADERAAALKDLET
                     QSRRSETQLFIETPYRNGAMLESLVSTLAPDTRVTVATDVTGEHESIRTMTVRAWAAL
                     MPEERALPKLPTVFALLAAPRGTAPRYAPSDARRKGGRDAGRPAAGRNFRPAQKKGRG
                     R"
     gene            complement(1866..>2349)
                     /locus_tag="ACZPB6_10015"
     CDS             complement(1866..>2349)
                     /locus_tag="ACZPB6_10015"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR1184837.1"
                     /translation="PGETPQDTAMRLSEMKARAIWDKHPGAVVIGSDQVADLNGVKLG
                     KPHTHENAVRQLKSMQGHDVVFTTALCVIDAEGRAERVKSQTTIRMRTLSDEAIEAYV
                     EREKPFDCAGAAKIERLGIALMESVKSDDPTSLIGLPLMALTTLLTRAGVEVLPGLDA
                     "
BASE COUNT          329 a          707 c          921 g          392 t
ORIGIN      
        1 tagggcttca cgaaggagac ggcggcgccg atgcgttcgg cgagcagcgt cgcgagcgtt
       61 ttcccgggcc tcaggaagta gacggcgttg ttcgtgcgca ggacgggcgc gcaggcgacg
      121 ccttcggagg cggagagcac catggcgccg atgacggtct gcagaaggtc cttggggcgc
      181 aggctgccgg ggatgtcgtg agggtcgtcc atgcggccgc ctcgggactt gtactcgacg
      241 gcgagcacgc cgccagccct gagggcgatg acggcgtcgg gctgtgcgta gaggcctccc
      301 gcctgaaagg tgcggcggtc ggattcgcgg cagaggagct cgccctcgtc tgcgggcagg
      361 aagggcacga ggcgcaggac ggggtcgtcg agcctgttga ccgcttcggc ggggaagaac
      421 accgcctcga cgccgatgcc gagcatgtcg gcgtgtcccg tggaaaggcc ctgcctcact
      481 atgaagggaa gcgtcttgag aaactcccac tcgggcatgg gcggcacgac gggcacggcg
      541 gggacgggcg cgggttcggg ttcgcgcctg aaggcgggct cgggcctcgg tgcgggatcg
      601 ggttcgggct tcggttcagg ggcgagcacc gcaacgtcgg gctcgcgtct gggttcggga
      661 gcggcctccc tgcgggcggc cgcgttcggg acgttcacgc gctcggtgta ggacgtgtgc
      721 ctgatctcct cgatgtcctc ggcgtcgttc tcgtctgact cgagcatttc gagtgcgagg
      781 cggtcagcct cgagcgggtc ggggcgcgag agcttcggag cggccctcgt cacggggccg
      841 tcgccttcgg gaaggccggc ggcgatcttc gagaggccga tgttgcgcct tgcctgcctg
      901 cgtctggcgg cgagccacat cgcaagggcg acgagaagca cgagcaggaa ccccgcggcc
      961 gtcatgatgc ggtcggggga gaactgctga agacctgcga ggatcgcgtt cacgttcatc
     1021 gtcctcgtcc ctttttctga gctggtctga agttcctgcc tgcggcgggg cggcctgcgt
     1081 ccctgccgcc cttgcggcgg gcgtcggacg gcgcatagcg cggtgccgtg ccgcggggcg
     1141 cggcgaggag cgcgaagacg gtgggaagct tgggcagcgc acgttcctcg ggcatgagcg
     1201 cggcccaggc gcggaccgtc atcgtgcgga tggattcatg ctcgcccgtc acgtctgtcg
     1261 cgacggtgac gcgggtgtcg ggggcgagcg tggagacgag gctctcgagc atcgcgccgt
     1321 ttcggtacgg cgtctcgatg aagagctggg tctcggagcg gcggctctgg gtctcgaggt
     1381 ccttgagggc cgcagcgcgc tcgtccgcat gcacgggcag gtagccgagg aagcggaagc
     1441 gctggccgtc gagtccgctc gccatgagcg tcatgagaat cgacgagggg ccgacccacg
     1501 gcacgacgcg caggccaagc tcctgggccc ttgcaacgat ctgtgcgccc ggatccgcaa
     1561 tgccgggaca gcccgactcg ctgacgatcg cggcatcctc gcccgcgagg acgggctcga
     1621 gccagcggtc gatcagggcg gggtcgggat catgtccgat ctcctcgatc ctgagctccg
     1681 agatggggcc gggatggccg agcagcttca ggtaggcgcg cgcattcttg gccgcttcga
     1741 cgaaaaagag gcggatgtct gcggcgcggg tgcgcacggc gggcggaatg gcggcttcca
     1801 gcgtgccgtc ggcgatgagg ttgggaaggc agtagatcgt gccggccatg ggaggtgctc
     1861 ctcaatcagg cgtcgaggcc ggggaggact tcaacgcccg cgcgggtgag aagcgtggtg
     1921 agcgccatga gcggcaggcc gatcagggag gtcgggtcgt ccgacttcac gctttccatg
     1981 agcgcgatgc cgaggcgctc gatcttggcg gcgccggcgc agtcgaaggg cttctcgcgc
     2041 tcgacgtagg cctcgatggc ctcgtcggag agcgtgcgca tgcggatcgt cgtctgggac
     2101 ttgacgcgct cggcgcggcc ttcggcgtca atgacgcaca gggccgtcgt gaacacgacg
     2161 tcgtggccct gcatgctctt gagctggcgg acggcgttct cgtgcgtgtg cggcttgccg
     2221 agcttgacgc cgttgaggtc ggccacctga tcggagccga tgacgacggc gccggggtgc
     2281 ttgtcccaga ttgcgcgggc cttcatttcg gaaaggcgca tcgcggtgtc ctgcggcgtt
     2341 tcgccgggc
//
LOCUS       DBMDTI010000311         2346 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10001_length_2346_cov_3.188864, whole genome shotgun sequence.
ACCESSION   DBMDTI010000311 DBMDTI010000000
VERSION     DBMDTI010000311.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10001_length_2346_cov_3.188864"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="ACZPB6_10020"
     CDS             <1..1143
                     /locus_tag="ACZPB6_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS-related protein"
                     /protein_id="HIR1184838.1"
                     /translation="LKNTERFITPELKNYEDQALSSKERSAALEKELYDGLVASLASH
                     VPALMAAARALAQLDVVGTLARHAVEHHWVRPTLTARPGIEIIKGRHPVVETTIESYV
                     PSDCRLGDGRRCLIITGPNMGGKSTYMRAVALITLLAWAGSFVPAESVTIGPVDRIHT
                     RIGASDDLARGRSTFMVEMTEAAAILHQATDRSLVLMDEIGRGTATFDGLSLAGAIAQ
                     ELVETTRSLTLFATHYFELTQLAVNLKEAANVHVSAAQTRSKIVFLHEIEEGPASRSY
                     GIAVAQLAGVPAPVVRRARAMLTKLENREAAIDSPQADLFGDGLFFEGQTSSAAVLEA
                     PAEPDPALREFAEAVAGLDVDSLTPREALAKLYEIREEAVKAAKGI"
     gene            1286..1975
                     /locus_tag="ACZPB6_10025"
     CDS             1286..1975
                     /locus_tag="ACZPB6_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR1184839.1"
                     /translation="MSKQDQLSMDEEALFRDIHHHILRQAKAGERIDTETELATRFNV
                     TRYKVRKALSVLSQMGVVDRAPKRGMTVNALGPKNLSAQIQVQMDIANFDIREFIEAR
                     QLIEVNIIPLAIRRMTPALLGRLEDAISRIEANAGNSAEADRWDREFHLLLLEACGNR
                     VLQVFSAALVTYFEKTTENLPQNDPQFFLDIAKNERLLLTAIKAGREDEAKALLSHQL
                     GEQVELLQGTR"
     gene            2095..>2346
                     /locus_tag="ACZPB6_10030"
     CDS             2095..>2346
                     /locus_tag="ACZPB6_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184840.1"
                     /translation="MTVPDEHPERCARAEDVHEHPANSASKPFLSRSHRCRRTEDKQA
                     QKSKNPSLFRNRGSWNGEPDDVLLSLEIQPTIIGDKVFHC"
BASE COUNT          473 a          807 c          655 g          411 t
ORIGIN      
        1 ctcaagaaca ccgagcgctt catcacgccc gagctcaaga actacgagga tcaggcactc
       61 tcctcgaagg agcgctccgc cgcgcttgag aaggagctct atgacggcct cgtcgcctcg
      121 ctcgccagcc acgtgcctgc tctgatggcc gccgctcgcg cgctcgccca gctcgacgtc
      181 gtgggcacgc tcgcgcgcca cgcggtcgag caccactggg ttcgcccgac gcttaccgcc
      241 cgtcccggca tcgagatcat caagggccgc caccccgtcg tcgagacgac gatcgagtcc
      301 tacgtgccga gcgactgccg cctcggagac ggccgccgct gcctcatcat cacgggcccc
      361 aacatgggcg gcaagtcgac ctacatgcgc gccgtagcgc tcatcacgct tctggcgtgg
      421 gcgggctcgt tcgtgcctgc agaatcggtc acgatcggtc ccgtcgaccg cattcacacc
      481 cgcatcggcg catccgatga tcttgcccgc ggccgctcga ccttcatggt cgaaatgacg
      541 gaggctgccg cgatccttca tcaggcaacc gaccgctcgc tcgtgctgat ggacgaaatc
      601 ggccgaggca cggccacgtt cgacggcctg tcgcttgcag gcgcgatcgc tcaggaactc
      661 gtcgagacga ctcgctcgct cacgctcttc gcgacccact acttcgagct cacgcagctt
      721 gccgtcaacc tcaaggaggc cgccaacgtg catgtctccg ccgctcagac ccgctcgaag
      781 atcgtcttcc tgcatgagat cgaggaaggt cctgcgagcc gcagctacgg cattgcggtg
      841 gcccagctcg cgggcgtgcc cgcacccgtc gttcgccgtg cgcgcgccat gctcacgaag
      901 ctcgagaacc gcgaggccgc catcgactcg cctcaagccg acctcttcgg cgacggcctc
      961 ttcttcgagg ggcagacctc gtccgccgcc gttctcgaag cccctgccga gcccgatccc
     1021 gcgcttcgcg agtttgcgga ggcggttgcg ggccttgacg tcgacagtct gacgccgaga
     1081 gaggctctgg cgaagctcta cgaaatccgc gaggaggccg tgaaggccgc caagggcatc
     1141 tgacgcagaa cagaaagttt tcagcaggct cgggacgaac cggaacctgc gaccggcggc
     1201 aggattgtgc gcccgaattt gcaaaccctc aaaaaacctc taaaattatc agaattttca
     1261 cgtttcggat ctcccgggag tcattgtgtc gaaacaggat cagctttcca tggatgaaga
     1321 ggcgctcttt cgcgacattc atcaccacat tcttcgccag gccaaggccg gcgaacgcat
     1381 tgatacggag accgaactgg ccacccgctt caacgtgacg cgctacaagg tgcgcaaggc
     1441 actgagcgtt ctctcccaga tgggcgtcgt cgaccgtgcg cccaagcgtg gcatgaccgt
     1501 caatgcgctc ggtcccaaga acctctccgc ccagattcag gttcagatgg acatcgcgaa
     1561 cttcgacatc cgcgagttca tcgaggcccg tcagctcatt gaggtgaaca tcatcccgct
     1621 cgcaatccgc cgcatgacgc ccgccctgct cggccgcctt gaggacgcga tcagccgcat
     1681 cgaagcaaac gccggcaatt ccgccgaagc tgaccgctgg gaccgcgagt tccaccttct
     1741 ccttcttgaa gcctgtggca accgcgtgct tcaagtcttc tccgccgcac tcgtaacgta
     1801 cttcgagaag acgaccgaga acctcccgca gaacgatccc cagttcttcc tcgacatcgc
     1861 gaagaacgaa cgccttctgc tcacggccat caaggctggc agggaggatg aagccaaggc
     1921 gctcctgagc catcagctcg gcgaacaggt cgaactcctt cagggcacgc gctaagcatc
     1981 ctgacgcaaa gcttccagac ggggcggtct tccgaaagaa gaccgccctt cttttcatcc
     2041 ttggtacgca cgaaaccctg cggtcgcacg cagttgcgct ctgaagagcc tcagatgacg
     2101 gtcccggacg agcacccaga acgttgcgcc cgcgccgaag atgtccatga gcaccctgca
     2161 aacagcgcca gcaagccgtt cctgagccgc agtcaccgat gtcgcaggac agaagacaag
     2221 caggcgcaga aaagcaaaaa ccccagtctc tttcgaaacc ggggttcttg gaatggtgag
     2281 cctgacgatg tcctactttc actggaaata caaccaacta tcatcggcga taaggtgttt
     2341 cactgt
//
LOCUS       DBMDTI010000312         2319 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10146_length_2319_cov_1.673580, whole genome shotgun sequence.
ACCESSION   DBMDTI010000312 DBMDTI010000000
VERSION     DBMDTI010000312.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10146_length_2319_cov_1.673580"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2147
                     /locus_tag="ACZPB6_10035"
     CDS             <1..2147
                     /locus_tag="ACZPB6_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184841.1"
                     /translation="VKALPAAESAAMPQINRKKDETTSDEKAVAKKPSAPVKMPERDA
                     TGRFLPSEEKAKKPRAKARAQQARTENVKKPSEKPEAKPAQKTAQKSEPKETHKAPKQ
                     VEPKVETKTDSQPAQKTQEAKAAKKPEGPVTSAAPAAPVPHVKPVLSLLMDEAPSGVM
                     TNMKADDRPAPGEVRGKPADETNEAPSSPVLRPSGSQSPNGSRIIDIEGEEDEEEDYD
                     DIPDPAVSVSESADDDEIDDDEPDDDDDQSSADRPVKKPVSVIRPAAEDPEPVELDSQ
                     GRPYHATEVLSGRRTPVRVKPPVEEPADPSRTVDLEAERIDAEHTARQALLRLHARTQ
                     PTRAELASMLVSRKIEQMERGNPAFEKDPSQEGDSDSVPDRNEDARSKGWNSLWGNGV
                     RDAAAEQNALVDVRRPPRYAFDEDERPQVERDEAYARRMDAWEKDVMAESESRDQRVS
                     CRGPKPGPRVPVRTGTHETLGGFPTPHQTRAWKAEGGEEEERESRLDIVRERLNRLHE
                     RWSGLEGCLRAALAVACVSSVAAFAAWTTAKSVVPDLTVMHTPVSVTAVVADEEIRNL
                     MLMTALLDARGIPTGRTAPQVDSVYLTGELRKIIVTDNEASRMSLPLDDRLFRAAVME
                     AADMAGAPVLSRAAVLAMPDPAHSTTGRTLDVTHLVIERLGLKNVSAAAAREVVSGLM
                     SPSGLFAAMTAVPNATTSAPSPSAEAAPVKNR"
BASE COUNT          472 a          795 c          725 g          327 t
ORIGIN      
        1 ctgtaaaggc cctgcccgcg gcggagtccg ccgccatgcc gcagatcaac aggaagaagg
       61 acgagacgac gtccgacgag aaggccgttg caaagaagcc gtccgccccc gtcaagatgc
      121 ccgagcgtga cgccacggga cgttttctcc cgtccgagga gaaggcgaag aagccccggg
      181 caaaggccag ggctcagcag gccaggaccg aaaacgtgaa gaagccctcg gaaaagcccg
      241 aagcgaagcc cgcgcagaaa acggcgcaga agtccgagcc caaggagact cacaaggctc
      301 cgaaacaggt cgagccgaag gtcgaaacca agacggatag ccagcccgct cagaaaacgc
      361 aggaagcgaa agccgcgaag aagcccgaag gcccggtcac gtccgccgca cccgcagcgc
      421 ccgtccccca cgtcaagccc gtcctctccc tgctgatgga tgaggccccg tccggggtca
      481 tgacgaacat gaaggcggac gaccgtccag cgcccggaga agtccgcgga aaacccgcgg
      541 acgaaacgaa cgaagcgcca tcttcgcctg tcctcagacc ttccggcagt cagtccccga
      601 acggttcccg aatcatcgac atcgaaggcg aagaagacga agaggaggac tacgacgaca
      661 ttcccgaccc ggcggtctcg gtttccgaat cggccgacga cgatgagatc gatgatgacg
      721 agcctgacga cgatgacgat cagtcttctg ccgaccgtcc cgtgaaaaag cccgtttccg
      781 tgatccgtcc cgcggccgag gaccccgaac ccgttgagct cgacagtcag ggccgtccct
      841 atcacgcgac cgaggttctc tccggccgcc gcacgcccgt gcgcgtcaag ccgcccgttg
      901 aggaacccgc cgatccgtca aggaccgtgg acctcgaggc cgagcgcatt gatgccgagc
      961 atacggcgag gcaggccctt ctcaggcttc acgcccgcac ccagcccacg cgtgccgaac
     1021 tggcttcgat gctggtctcc aggaagatcg agcagatgga gcgcggcaat cctgcgttcg
     1081 aaaaggatcc ctctcaggaa ggggattcgg attccgttcc cgaccggaac gaggacgccc
     1141 gcagcaaggg ctggaactcg ctctggggaa acggcgttcg cgatgccgcg gccgagcaga
     1201 acgcgttggt cgacgtgcgt cgtccgccgc gctatgcgtt tgacgaggac gaacgtcctc
     1261 aggtcgagcg tgacgaagcc tatgcccgcc gcatggatgc ctgggaaaag gacgtgatgg
     1321 cggagagtga atcccgcgac cagcgcgtgt cgtgccgcgg cccgaagccc ggtccccgcg
     1381 ttcccgtccg cacgggcacg cacgagacgc tcggcggctt tccgaccccg caccagactc
     1441 gcgcctggaa ggccgaaggc ggtgaagaag aggagcgcga atcgcgtctt gacatcgttc
     1501 gtgaaaggct caaccgcctt catgaacgct ggtcgggcct cgaggggtgt ctgagggcgg
     1561 cgcttgccgt tgcctgcgtc tcttccgtgg ccgcctttgc cgcctggacg acggcgaagt
     1621 cggtcgtgcc cgacctcacg gtcatgcaca cgcccgtgtc ggtcacggcc gtcgtggccg
     1681 acgaggagat caggaacctg atgctgatga cggcgctcct tgacgccagg ggaattccga
     1741 cgggccgcac ggctcctcaa gtcgacagcg tgtacctgac gggcgaactc agaaagatca
     1801 tcgtcacgga caacgaagcc tcacgcatgt cgctgccgct tgacgaccgt ctcttcaggg
     1861 ccgccgtgat ggaggccgcc gacatggcgg gtgcgccggt cctctcccga gccgccgtgc
     1921 tggccatgcc cgaccccgct cactccacga cggggcgcac gcttgacgtg actcacctcg
     1981 tcatcgagcg cctcggcctc aagaacgtgt cggccgctgc cgcacgcgaa gtggtctcgg
     2041 gcctcatgag tccctcgggg cttttcgccg ccatgacggc cgtccccaac gccacgacgt
     2101 ccgcaccttc gccctccgcc gaagctgctc ccgtcaaaaa ccgctgacaa acaacgctga
     2161 caacagccat tcgataaaga gcacagacat gaatcaagtg accccgcctt cgggcgtcaa
     2221 gggctccgcg cccgtctcgc ccgctccgaa gagcaacgtc gccgtggagc ccggagagca
     2281 gttctacgag cgtgcgctcc ggaggcgcat ccgctcctg
//
LOCUS       DBMDTI010000313         2309 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10200_length_2309_cov_2.516304, whole genome shotgun sequence.
ACCESSION   DBMDTI010000313 DBMDTI010000000
VERSION     DBMDTI010000313.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10200_length_2309_cov_2.516304"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            635..1534
                     /gene="hisG"
                     /locus_tag="ACZPB6_10040"
     CDS             635..1534
                     /gene="hisG"
                     /locus_tag="ACZPB6_10040"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR1184842.1"
                     /translation="MTETNRLRIAMQKSGRLSDDSQDLLERCGLKVRYHAQRLLAMAE
                     NMPVEILRVRDDDIPGLVMDGVVDLGIIGENVLEETVLARRSQGQSTDFRMLRRFDFG
                     GCRLSIAIPADQEWAGLESLDGCRIATSYPQLLKRCFDERGLTFKPCLLTGSVEVAPR
                     AGLADAICDLVSSGATLEANGLREVEVVYRSKACLIAKSEPLDADKEALVEKLLVRMQ
                     GVSRARECKYIMLHAPKAKLAEVTALLPGAEHPTILPLAGSDDHVALHMVSSESLFWE
                     TMEELKALGASSILVLPIEKMME"
     gene            1540..>2309
                     /gene="hisD"
                     /locus_tag="ACZPB6_10045"
     CDS             1540..>2309
                     /gene="hisD"
                     /locus_tag="ACZPB6_10045"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR1184843.1"
                     /translation="MSQFNFNDVIEWGALNETEREEILRRPAVMAGDRIKAVVEGILE
                     AVRERGDEALREFSAKFDRTEVKDFRVSAEEVDAAVARLPEDFMAALKTAAANIEKFH
                     SAEVLEPVTVETMPGVVCQQVTRPIGAVGLYIPGGTAPLFSTVLMLGIPAKIAGCGRV
                     ILCSPPPIADPILAAARLCGIDEIYQVGGAQAVAAMAFGTATIPRVGKIFGPGNAYVT
                     EAKRQVSSRPDGAAIDMPAGPSEVLVIADEGADPAFVAA"
BASE COUNT          442 a          694 c          750 g          423 t
ORIGIN      
        1 ctcctcctcg taggtggagt agaggtaggc ggtgttggtc gcgaattcgg cggggcgaga
       61 agtccgttct taagtccttc ttccgatcgt ccgaacagct gaggcaggcc gttcggtttt
      121 tccgtgagtt ttcggttttt gggggcattg agtgcgaaag tgaaagattt caagaaaaaa
      181 gtgagacttt tagggagaac gtcctaggca cttctgccca gaaggtctga tgattcgcac
      241 gagaaggcga ttctcctgct gcgagggtct gaggagggct tgatgcgcgg gatcaacgtg
      301 ccataatgga cgaaagtcag gcgcagtcat gcctgacgag aaacgaattc aagcgcaaaa
      361 aacacagccg gagcacgaaa aagtgtacct cttcctcctt cgccgccatc accaccatac
      421 gtaagacatc cctcgggcgc ggaaacgctg tcgggaggat gggttcatgc tttcggcggt
      481 gcggagtgag actccagcag gacaatcaag caaagccccc ggaagcacgc cttccggggg
      541 ctttttcaca atccggggtg cggatgtcga ggggctggac acgaagctcc gcttcggcga
      601 gcaacagtca tcaaatcaaa tttcagggaa gcaaatgacc gaaaccaatc gtctgaggat
      661 tgcaatgcag aagtcgggac gtctttccga cgattcgcag gatcttcttg aacgctgcgg
      721 actcaaggtt cgctaccacg cgcagcgcct gctggcgatg gccgagaaca tgccggtcga
      781 aatcctgcgc gtcagggatg atgacattcc cggcctcgtg atggacgggg tcgtggatct
      841 gggcatcatc ggcgaaaacg tgcttgagga gacggttctt gcacgccgca gccagggcca
      901 gtccacggac ttcaggatgt tgcgccgctt cgactttggc ggctgccgcc tctcgatcgc
      961 gatcccggcc gaccaggagt gggcggggct tgagagcctt gacggctgcc gcatcgcgac
     1021 gagctatccg cagcttctca agcgctgctt cgacgagcgc ggcctcacgt tcaagccatg
     1081 cctgctgacg ggctccgtcg aggtggcacc gcgcgcgggg ctggccgacg cgatctgtga
     1141 cctcgtgagc tcgggcgcga cgcttgaagc gaacggcctg cgcgaagtgg aggtggtcta
     1201 ccgttcgaag gcctgcctca tcgcgaagag cgagccgctc gacgccgaca aggaggccct
     1261 ggtcgagaag cttctcgtgc gcatgcaggg cgtctcaagg gcccgcgaat gcaagtacat
     1321 catgctgcac gccccgaagg cgaagctcgc cgaagtaacg gcgcttctgc ccggcgcgga
     1381 gcacccgacg atcctgccgc ttgcggggtc cgacgaccat gtggcccttc acatggtgag
     1441 cagcgaaagc cttttctggg aaacaatgga agagctcaag gcgctcggcg ccagctcgat
     1501 tctcgtcctg ccgattgaaa aaatgatgga gtagtcaaga tgtctcagtt caacttcaac
     1561 gacgtgatcg aatggggcgc gctcaatgaa acggagcgcg aggagattct ccgacgcccc
     1621 gccgtgatgg cgggcgaccg gattaaggcc gtggtcgagg gcattctcga ggccgtgcgc
     1681 gaacgcggtg acgaggccct tcgcgaattc tccgcgaagt tcgaccgcac cgaggtgaag
     1741 gacttcaggg tctcggccga ggaggtcgac gccgccgtcg cacgtctgcc cgaggacttc
     1801 atggccgctc tgaagacggc cgccgccaac atcgagaagt tccacagcgc cgaagtgctt
     1861 gagcccgtca cggtcgagac gatgcccggc gtcgtctgcc agcaggtcac gcgtccgatc
     1921 ggcgcggtgg gcctctacat tcccggcggc acggctccgc tcttttccac ggtgctgatg
     1981 ctcggcattc ctgccaagat cgcgggctgc ggccgcgtca tcctctgctc gccgcctccg
     2041 atcgccgatc cgatcctcgc cgccgcgcgc ctttgcggca ttgacgagat ctatcaggtg
     2101 ggcggcgccc aggccgtggc cgcgatggcc ttcggcacgg cgacgatccc gcgcgtgggc
     2161 aagatcttcg gtccgggcaa cgcctacgtg accgaggcca agcgtcaggt gagcagccgt
     2221 cccgacggcg ccgccatcga catgcccgcg ggtccctccg aggtgctcgt gatcgccgac
     2281 gaaggcgccg atccggcctt cgttgcggc
//
LOCUS       DBMDTI010000314         2289 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10315_length_2289_cov_2.219835, whole genome shotgun sequence.
ACCESSION   DBMDTI010000314 DBMDTI010000000
VERSION     DBMDTI010000314.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10315_length_2289_cov_2.219835"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="ACZPB6_10050"
     CDS             <1..1222
                     /locus_tag="ACZPB6_10050"
                     /inference="COORDINATES: protein motif:HMM:NF027651.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HIR1184844.1"
                     /translation="NELGWGGWWFWDPVENASFIPWLTTTALMHALIQTRRRGQLRRL
                     TVFLTLVGFALCLLGTFLVRSGAMQSVHAFATDPGRGIALQVLAIVLLVPAAFLYCAR
                     IEPVCRTEEAPMNGSDALMTGAVFLLMAAAFAVLFGTLYPLGYEVFAGRTLTVGAPYF
                     NNFFAPMTLIAAALAGAAQLGLRNPVRWAVCAAASVAAGAAVALLTDPRSPVMTGFAV
                     AAAVWLVVTAVSHCLPFPARRMSLAALLAHVGIAVSIAGAVGVEQYESEALVRMGPGL
                     GKPVDDVVFVYTETRFVNNHSYFGQQGVIEVLRLDDESLITTLRPERQTFVANGMQMS
                     AAGIDHGFLRDYYVSMGNKLTDTEWLVRLSIKPLAGWIWGGGAVMILAALIILFTRRR
                     ETETAAQELEAPSK"
     gene            1219..1644
                     /locus_tag="ACZPB6_10055"
     CDS             1219..1644
                     /locus_tag="ACZPB6_10055"
                     /inference="COORDINATES: protein motif:HMM:NF015853.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR1184845.1"
                     /translation="MIFMTRRTLVRTAALETGMMLAAPLLAAETKPLTGAELQKEAVS
                     ISKVLRCPVSPNLTLYDSESAIANELKGVIYEKLRAGETREQIFDFMVARYGEQIRYE
                     PDMNAGTAALWAAPWVALGACGLFLAARMRRKGAKADRA"
     gene            1716..>2289
                     /locus_tag="ACZPB6_10060"
     CDS             1716..>2289
                     /locus_tag="ACZPB6_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR1184846.1"
                     /translation="MFKKALIALAFVIGAQCASAALPAFNLKFEKNGQPIAEKPLVAR
                     DFIEFRTARGFPLDGYKDLAGNPQSLSQFKDKLVIVDVWGSWSATCQRSAPLMAEFQA
                     KYNKPESRIRFVSISLDKNPKRVTKFVKKTKLPETFTSWHDPDLVIPATLPADVLPGF
                     FVLDGHGHLVGFVRGFVDWADPAVPAYFEKL"
BASE COUNT          363 a          832 c          693 g          401 t
ORIGIN      
        1 caacgagctc ggctggggcg gctggtggtt ctgggatccc gttgagaacg cgtccttcat
       61 tccgtggctg acgaccaccg cgctcatgca cgcgctcatc cagacgagaa ggcgagggca
      121 actcaggcgc ctcaccgtct tcctcacgct cgtgggcttc gcgctctgcc tgctcggcac
      181 cttcctcgtg cgctcgggcg ccatgcagtc cgtgcatgcg ttcgcgacgg atccgggccg
      241 cgggatcgcg cttcaggtgc tcgccattgt gctgctcgtg cccgccgcct tcctctactg
      301 cgcgcgcatc gagcctgtct gcaggaccga ggaggcgccg atgaacggct cggacgccct
      361 gatgacgggc gccgtgttcc tgttgatggc cgccgccttc gcggtgctct tcggcacgct
      421 ctatccgctt ggatacgagg tcttcgcggg ccgcacgctg acggtcggcg cgccgtactt
      481 caacaacttc ttcgcgccga tgactctcat cgccgcggcg ctcgcgggcg ccgcccagct
      541 cggcctcagg aatccggtcc gctgggccgt ctgcgcagcg gcctccgtcg ccgcaggcgc
      601 agccgtcgcg ctcctcaccg acccgcgctc gcccgtcatg acgggctttg cggtcgcggc
      661 cgccgtctgg ctcgtcgtca cggccgtctc gcactgcctg cctttcccgg cccgccgcat
      721 gagccttgcc gcgctgctcg cgcacgtggg catcgccgtc tccattgcgg gtgcggtcgg
      781 cgtcgagcag tacgagagcg aggcgctcgt gcgcatggga cccggcctcg gcaagcccgt
      841 cgacgacgtg gtcttcgtct atacggagac gcgcttcgtc aacaaccact cctacttcgg
      901 acagcagggc gtgatcgagg tgctcaggct tgacgacgag tccctcatca cgacgctcag
      961 gcccgagcgc cagaccttcg tcgccaacgg catgcagatg tccgcagccg gcatcgacca
     1021 cggcttcctg cgcgactact atgtctcgat gggcaacaag ctcaccgaca ccgagtggct
     1081 cgtcaggctc tccatcaagc cgctcgccgg ctggatttgg ggcggcggcg ccgtcatgat
     1141 tctggccgcg ctcatcattc tcttcacgcg ccgtcgcgaa accgagacgg cggcgcagga
     1201 gttggaggct ccttccaaat gatcttcatg acacgcagga ccctcgtgag aacggcagcg
     1261 ctcgagacgg gcatgatgct cgccgcgccg ctcctcgccg ccgagacgaa gccgcttacg
     1321 ggggccgagc ttcagaagga ggccgtgagc atctccaagg tgctccgctg cccagtctcg
     1381 cccaacctca cgctctacga cagcgagagc gccatcgcca acgagctcaa gggcgtcatc
     1441 tacgaaaagc tgcgcgcggg cgaaacccgc gagcagatct tcgacttcat ggtcgcccgc
     1501 tacggcgagc agatccgcta cgagcccgac atgaacgcgg gcaccgccgc cctctgggcc
     1561 gcgccctggg tagcgctcgg cgcctgcggg ctcttcctcg ccgcgcgcat gcgcaggaag
     1621 ggtgccaagg ccgacagggc ctgagacgcc tcgtccagcc tcggaattca aaagacagaa
     1681 tcgacagaca gcattcagag aggagattca ccgacatgtt caagaaagcc ctgatcgcgc
     1741 tcgccttcgt gatcggcgcc cagtgcgcct cggccgcgct tcccgccttc aacctgaagt
     1801 tcgagaagaa cggccagccc atcgccgaaa agccccttgt cgcccgcgac ttcatcgagt
     1861 tccgcaccgc ccgcggcttc ccgctcgacg gctacaagga cctcgcaggc aatccgcagt
     1921 cgctttcgca gttcaaggac aagctcgtga tcgtcgacgt ctggggttcc tggagcgcca
     1981 cctgccagcg cagcgcgccg ctcatggccg aattccaggc gaagtacaac aagcccgaga
     2041 gccgcatccg cttcgtttcc atctcgctcg acaagaaccc gaagcgcgtg acgaagttcg
     2101 tgaagaagac gaagctcccc gagaccttca cctcctggca tgatcccgac ctcgtcattc
     2161 ccgcgacgct tcccgccgac gtgctgcccg gcttcttcgt cctcgacggc cacggccacc
     2221 tcgtgggctt cgtgcgcggc ttcgttgact gggccgatcc cgccgtgccc gcctacttcg
     2281 aaaagctcg
//
LOCUS       DBMDTI010000315         2283 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10343_length_2283_cov_1.988543, whole genome shotgun sequence.
ACCESSION   DBMDTI010000315 DBMDTI010000000
VERSION     DBMDTI010000315.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10343_length_2283_cov_1.988543"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1862)
                     /locus_tag="ACZPB6_10065"
     CDS             complement(<1..1862)
                     /locus_tag="ACZPB6_10065"
                     /inference="COORDINATES: protein motif:HMM:NF023835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase-associated domain-containing
                     protein"
                     /protein_id="HIR1184847.1"
                     /translation="MFKVDGEKIRNLFAEHPAGYIHGDLRALTGRIVSEAGKLVAHQM
                     RTVIERTIPEYDEARPLIAINTWGQPVVDESLFWRERRESGRFMRQELRGPSASDPVG
                     RGLHNMFSGFITHQKLSEAPCELEALYDAWLIAGRLVEAGAVVPQIYRPIDDIFAVRW
                     IPAVMDDEVKAVTEAVGAAFSHVGPEFLRISKAPVAMHPLLLGEIVLELFIQSYIVAA
                     WHTHVDETGDAVPEHHALFGCEPVDCDGDSALKAVGMRLEGWGAPVFLKTESHRVKGI
                     VIVHDWVAAATAAKREGGDDVGARAGSATLAAMFGAREAQPEPVPLECPTFIPYQGAD
                     EAEEPRYDEAIPEDVEMPDFDPHQAVAIEMRFSVDGGGAQESVGLADILDLPQHKALR
                     FDCLKLMSRLSGYCPELTELLKNRASTTVVPLDELADLLFKAIPTLNLLGVQTILPRT
                     LRNLLRPQATLDLDMKENVFESRGLFDIVDAMTFDWRLAAGKYTMSEEEFEWLSEQAG
                     RIVRFRDAYMYVDKRLLSSIRRELHGQRKLTKAELIRAALSGGIRANGVRLSARLQAA
                     FDRLFAEKTFDVPDTIQATLRPYQVRGYSWLMRNLRADMGAIIADDMGLGKTLQV"
BASE COUNT          467 a          720 c          665 g          431 t
ORIGIN      
        1 acttgaagcg tcttgccgag gcccatgtcg tcggcgatga tggcccccat gtccgcgcgc
       61 agattgcgca tgagccagga atagcctcgc acctgatagg gacgcaacgt cgcctggatc
      121 gtatcgggca catcgaacgt cttttcggca aaaagcctgt cgaaagccgc ctgcaggcgt
      181 gcactcaaac gcacgccgtt ggcgcggatg ccgcccgaga gcgcagcgcg gatgagctcg
      241 gccttcgtga gcttgcgctg accgtgaagt tcgcggcgaa tggaactcag gagccgcttg
      301 tcgacgtaca tgtacgcatc gcgaaagcgc acgatgcgcc ctgcctgctc ggagagccac
      361 tcgaactctt cttccgacat cgtgtacttg cccgccgcca gacgccagtc gaacgtcatc
      421 gcatcgacga tgtcgaaaag acctcgactt tcgaagacat tctccttcat atccagatcg
      481 agcgtcgcct gagggcgcaa gaggttccgg agcgttctcg gcaaaatggt ctgcacgccg
      541 agtagattga gcgtcgggat tgccttgaaa aggaggtccg ccaactcatc aagcggcaca
      601 accgtcgtcg aggcgcggtt tttgagaagc tccgtgagct ccggacagta gcctgaaaga
      661 cgcgacatca gcttcaggca gtcaaagcgc agcgccttgt gctgcggcag atcgagaatg
      721 tccgcgagtc caacgctctc ctgcgcacca ccgccgtcaa ccgagaatcg catttcgatg
      781 gcgactgcct ggtgcgggtc aaagtccggc atctccacgt cttcaggaat cgcttcgtcg
      841 taccgcggct cttcggcctc gtccgccccc tgatacggaa tgaatgtcgg gcactcaagc
      901 ggtacaggtt caggttgtgc ctcacgcgca ccgaacatcg ccgccaaggt cgcgcttcct
      961 gcccgcgcgc caacatcgtc accgccctcg cgctttgcgg ccgtcgctgc cgcaacccag
     1021 tcgtgcacga tgacgatccc cttgacgcgg tgcgactcgg tcttgagaaa gacgggcgcg
     1081 ccccagcctt caaggcgcat cccgaccgcc ttcaaggcgg aatcaccgtc acagtcgacg
     1141 ggttcgcacc cgaagagcgc gtgatgctcg ggaaccgcgt cacccgtttc gtcgacatgg
     1201 gtgtgccatg cggcgacgat gtagctctgg atgaagagtt cgagcacgat ttcgccaaga
     1261 agcagcggat gcatcgcaac aggcgccttc gaaatgcgaa ggaactccgg accgacgtgc
     1321 gagaaggctg cgccgacggc ttcggtgacc gccttcactt catcgtccat gacggccgga
     1381 atccaacgca cggcgaagat gtcatcaatc ggtctgtaga tctgcggcac gactgcgccc
     1441 gcctcgacga gacgaccggc gatgagccag gcatcgtaga gagcctccag ctcgcacggc
     1501 gcttcagaaa gcttctgatg cgtgatgaag cccgaaaaca tattgtgcag gcctcgtccg
     1561 accggatccg acgcactcgg tccgcgtagc tcctggcgca tgaagcggcc gctctcacgt
     1621 cgttcgcgcc agaagagcga ttcgtccaca acaggctgcc cccaagtatt gatcgcgatg
     1681 agcggccgag cctcgtcata ctcgggaatc gtgcgttcga tgacggtacg catctgatgt
     1741 gcaacgagct tccctgcttc gctcacgata cgccccgtga gcgcacgaag gtcgccgtgg
     1801 atgtagcccg ccggatgctc ggcaaaaagg ttgcgaatct tctcaccatc aaccttgaac
     1861 atgcggaagg tttgctttcg cagatcttcg agccagccga catccgattc tccatccgga
     1921 tgcaggtcag tgacgaagcc tcgttcgtcg agtccccgga tcaaatcgtc ccaggatggc
     1981 atctcggcct caactgcctt ttcgagctcg acgccgcggc tttccatttc ggcaatcagg
     2041 tcgatgccac gcaacgtaaa gagcaggaag ggattggcat caatttcttc gctcagcttg
     2101 tagatggtgg ctgcaatgtg cttgcacggc accgcccaat ccggacacgt gcacgacatc
     2161 tccagatcct tccagctcgt cgggaaaatg cgaatgccga gcgactcggc aatgtcggca
     2221 atttcgggac tcagttctct cgcggcgagg cgcgccacga tgagaggaga ttcggcaaga
     2281 cgg
//
LOCUS       DBMDTI010000316         2282 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10347_length_2282_cov_2.719853, whole genome shotgun sequence.
ACCESSION   DBMDTI010000316 DBMDTI010000000
VERSION     DBMDTI010000316.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10347_length_2282_cov_2.719853"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="ACZPB6_10070"
     CDS             complement(<1..315)
                     /locus_tag="ACZPB6_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024996.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR1184848.1"
                     /translation="MQFGKTVAALASLLAAGTCAAAGVKVYGAIDTGLTYKHVAESGG
                     NSLEMTSGNFAGSRLGLKCSEDLGNGLPVGFILENGSSSDSGALGKDGSIFNRESQIY
                     LKT"
     gene            458..533
                     /locus_tag="ACZPB6_10075"
     tRNA            458..533
                     /locus_tag="ACZPB6_10075"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:491..493,aa:Gly,seq:gcc)
     gene            538..611
                     /locus_tag="ACZPB6_10080"
     tRNA            538..611
                     /locus_tag="ACZPB6_10080"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:570..572,aa:Cys,seq:gca)
     gene            619..694
                     /locus_tag="ACZPB6_10085"
     tRNA            619..694
                     /locus_tag="ACZPB6_10085"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Gly,seq:gcc)
     gene            782..869
                     /locus_tag="ACZPB6_10090"
     tRNA            782..869
                     /locus_tag="ACZPB6_10090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:816..818,aa:Leu,seq:taa)
     gene            1092..>2282
                     /locus_tag="ACZPB6_10095"
     CDS             1092..>2282
                     /locus_tag="ACZPB6_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR1184849.1"
                     /translation="MQRRQLIGAAATAGSVTIAPALLTGCAAAPKSEPAATVATLPEG
                     ASAHVTAVAPTGAYRGCVLDGTGVRRWLGIPFIKNPYEPVRRYLKPEPYGAHEGVMDC
                     LKHGSIPLQPNPDKNVEKKMVGGDGPLCLNIWAPKAGENLPVMVWVPGGGSISCAQND
                     ERFDGTNFARDGVVLVTLAYRVNVDGFLKLRGGDSGNGNRDILEGLRWVKANIAAFGG
                     DPDKITAFGQSAGGTHLVDMIASPYTKGLLAGAVIQSPSAVAQWRDAAQAEEAAKLVC
                     DAIGCEPTREAMLKIPNESLAAFGSLAGRLAKNAAWARSTNGNIALFKAYVGDDFMPV
                     RPVDAIAAGACEGLAVITGSTRSEWRHYIVPNGLIAKVNEAAVKALCVNAGLSADLPE
                     AYRRA"
BASE COUNT          446 a          716 c          697 g          423 t
ORIGIN      
        1 ggtcttgaga tagatctgcg actcgcggtt gaagatcgag ccgtccttgc cgagcgcgcc
       61 gctgtcggag ctggagccgt tttcaagaat gaagccgacg gggaggccgt tgcccaggtc
      121 ttcggagcac ttgaggccga ggcgggaacc ggcgaagttg cccgaagtca tttccaggct
      181 gttgccgccg ctctcggcga cgtgcttgta ggtgaggccg gtgtcgatgg cgccgtagac
      241 cttgacgcct gcagcggcac aggtgcctgc ggcgaggagc gacgcaaggg cagcgaccgt
      301 ttttccaaac tgcatgatgt tgtctttgac gaagtctgct gaagggtgaa tgaggagagc
      361 cttcggaggt ccgaggggcg gaatggtttt aggtcttgat ttttttgagg aattcttgac
      421 atttccaaaa acctatgtaa aatacgactt ctcagatgcg ggagtagctc agttggtaga
      481 gcgcaacctt gccaaggttg aggtcgcgag ttcgagactc gtctcccgct ccaagatggc
      541 gcgatagcaa agtggctatg caccggattg caaatccgtt cacggcggtt cgattccgcc
      601 tcgcgcctcc agacatctgc gggagtagct cagttggtag agcgcaacct tgccaaggtt
      661 gaggtcgcga gttcgagact cgtctcccgc tccagaattc aagaaaaaag gaagcggcag
      721 gcttcctttt ttcgtttgta cgctccgaaa tccagagagc gtccgaagca tcggcttcgg
      781 tgccggaatg acggaattgg tagacgtagc ggacttaaaa tccgccgcaa caaaattgcg
      841 tacgggttcg acccccgttt ccggcaccat cagacatcag gccccgcgga cacccgcggg
      901 gccttttttc ttgttgatcc gcccgtcccg acatctgaat tgttcggctt acgcgcgtaa
      961 cttgcccgcg cgcaagcctg caatgattag gcccgtctga acaggcgcac atttctaaaa
     1021 tggacgggaa ccatcaacga ccggacggcg tcaggctcct cccctcgccg ctcctcatga
     1081 gatccaagac catgcaaaga agacaactca tcggagccgc cgcaactgcg ggctccgtca
     1141 cgatcgcccc cgcccttctg acgggctgtg ctgccgcccc taagtccgaa cccgccgcaa
     1201 ccgttgcgac gctccccgaa ggtgcgtccg cccacgtgac cgccgtggcg ccgacgggtg
     1261 cctacagggg ctgcgtgctt gatggcacgg gcgtgcgccg ctggctcggc attcccttca
     1321 tcaaaaatcc ttacgagccc gtgcgccgct acctgaaacc cgaaccctac ggcgcgcacg
     1381 agggcgtcat ggactgcctg aagcacggca gcattccgct ccagcccaat cccgacaaga
     1441 atgtcgagaa gaagatggtg ggcggcgacg gtccgctctg cctgaacatc tgggcgccga
     1501 aggcgggtga gaaccttccc gtcatggtct gggtgccggg cggcggatcg atcagctgtg
     1561 cccagaacga cgaacgtttc gacggcacga acttcgcccg tgacggcgtc gtgctcgtca
     1621 ccctcgccta ccgcgtgaac gtcgacggct tcctcaagct caggggcgga gactcaggca
     1681 acggtaaccg cgatattctc gaaggcctgc gatgggtcaa ggccaacatc gcggccttcg
     1741 gcggcgaccc cgacaagatc acggccttcg gccagtcggc gggcggcacc cacctcgtcg
     1801 acatgatcgc ctctccgtac acgaagggac tgctggcagg tgccgtcatc cagagcccgt
     1861 cagccgtcgc acagtggcgg gatgctgccc aggccgagga agccgcaaag ctcgtctgcg
     1921 acgccatcgg ctgcgaaccc acccgcgaag ccatgctgaa gatacccaat gaaagccttg
     1981 cggccttcgg atcgcttgcg ggcaggctcg cgaagaacgc cgcatgggcc cggagcacca
     2041 acggcaacat cgcgctcttc aaggcatacg tgggcgacga cttcatgccg gtgcgtcccg
     2101 tggacgccat cgctgccggc gcgtgcgagg gtctcgccgt gatcaccggt tccacccgtt
     2161 ccgagtggag acactacatc gttcccaacg gcctcatcgc caaggtgaac gaagctgcgg
     2221 tgaaggcgct ctgcgtcaac gccgggcttt ccgccgatct gcccgaagcc taccgccgtg
     2281 ca
//
LOCUS       DBMDTI010000317         2278 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10374_length_2278_cov_2.247129, whole genome shotgun sequence.
ACCESSION   DBMDTI010000317 DBMDTI010000000
VERSION     DBMDTI010000317.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10374_length_2278_cov_2.247129"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="ACZPB6_10100"
     CDS             <1..436
                     /locus_tag="ACZPB6_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012354.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1184850.1"
                     /translation="APRNLQRAVSRLGMPQATASRSLTKLREAFEDELFTRCSGGLTP
                     TPKALALEPKVAAVIASFEDLFVEETVDPASLVREFRIACADHAMFYIGLAVTLASRT
                     APGVTFRLSPNGDDWVVELQRGDLDFLNNLATEVGGFLRLFL"
     gene            433..924
                     /locus_tag="ACZPB6_10105"
     CDS             433..924
                     /locus_tag="ACZPB6_10105"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1184851.1"
                     /translation="MIFPIERAPEGCACLPLSSCRSVLVVRSGHSLARRFDEAGALTL
                     EECLAHRYVEVRPGPEWVLRAMDASVSEAWIARPRAVRTPYFFSAVQMVRRSDLCLTC
                     SETLAREVAGTDEFRILPLPEECSKKWVPKLVWHERGHRDPAMQWLRSVITSLVHEQD
                     SGG"
     gene            complement(1140..>2278)
                     /locus_tag="ACZPB6_10110"
     CDS             complement(1140..>2278)
                     /locus_tag="ACZPB6_10110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184852.1"
                     /translation="IKFYYEQVKVLCDNAYGAITWLESLGMKFKDNVIQIFGALYPRS
                     HIPALPKGQGYGTVLTKAAKELGVQVRTGLGVTEIIREEQFAGRVIGVKVQPAKGPAR
                     FIRATKAVVLAAGGFSGNRYLRELHDPRVKGLGTDNLPGSTGEVAMAAVRVGAYLVGM
                     DFIQSTPGAPAGKKMKLLLNFNVNGSIYVDKRGNRIVNEGERRDVIRDAVLGTPERYG
                     YTVCDNTNFMSYDAVNRKAIMEGIKINEAWTAPTIRELAEKMGVDPDGLEKTIKRYNE
                     VFVKNGRDDDFHKQPVNLTKRIEDGPFWACYTGMTVHHTMGGINTNVKAQALDWEGKV
                     IPGLYAAGEITGGIHGTNRVGGNAVLDCFVFGQIAGDNAAKEVA"
BASE COUNT          356 a          669 c          808 g          445 t
ORIGIN      
        1 cgcgccgcgc aaccttcagc gggccgtctc gcggctcggc atgccgcagg cgacggcgtc
       61 gcgctcgctc acgaagcttc gcgaggcctt tgaggacgag ctcttcacgc gatgctcggg
      121 cggcctcacg cccacgccaa aggcgctcgc gctcgagccg aaggtggcgg ccgtgatcgc
      181 aagcttcgag gacctcttcg tcgaggagac cgtcgatccc gcgagtctgg tgcgcgaatt
      241 caggatcgcg tgcgcggatc atgcgatgtt ctacatcggg ctcgccgtca cgctcgcttc
      301 cagaacggcg ccgggcgtca cgttcaggct ctcgcccaac ggagacgact gggtcgtgga
      361 gctccagaga ggcgatctgg acttcctaaa caacctcgcc actgaagtgg gaggcttcct
      421 gcggctgttt ctgtgatctt tcccatcgag cgcgcgcccg aaggctgcgc atgcctgccg
      481 ctttcgtcgt gcaggagcgt gctcgtcgtg cgaagcggtc attcgctggc ccgaaggttc
      541 gacgaggcgg gcgcactcac gctcgaagag tgtcttgcgc acagatacgt ggaagtccga
      601 ccgggccccg aatgggtgct tagggcaatg gacgcgtcgg tttccgaggc ctggatcgca
      661 aggccgcgtg cggtgagaac gccgtacttc ttcagcgccg tgcagatggt gaggcgttcc
      721 gacctctgct tgacctgttc cgagacgctt gcgcgtgaag tcgcgggcac ggatgaattc
      781 aggattctgc cgcttcccga ggagtgttcg aagaaatggg ttccgaagct cgtctggcac
      841 gagcgcggac atcgggatcc cgccatgcag tggctgagga gcgtgatcac ctcgctcgtg
      901 cacgagcagg attcgggtgg gtgaggaaag agctgcagat ggaaatgaag ggccggttcc
      961 gtgaagaacc gacccctttc cgtacgtctg cctgccgggc cgttggagga gcgtgggtcc
     1021 ggccgggcgg gcgtgcatgc atgcggcgga gaagtccgca tgcgtgaggt cgatgccggg
     1081 cgccgtgagg agagaggacg gcgcccggca cttctcagtc gtgagacctg gaggtctcgt
     1141 caggcgactt ccttcgcggc gttatcgccg gcgatctggc cgaagacgaa gcagtcgaga
     1201 acggcgttgc cgccgacgcg gttcgtgccg tggatgccgc ccgtgatttc gcctgcggcg
     1261 tagaggcccg gaatgacctt gccttcccag tcaagggcct gagccttgac gttggtgttg
     1321 atgccgccca tcgtgtgatg gaccgtcatg ccggtgtagc aggcccagaa gggaccgtcc
     1381 tcgatgcgct tggtgaggtt gacgggctgc ttgtggaagt cgtcgtcgcg gccgttcttg
     1441 acgaagacct cgttgtagcg cttgatcgtc ttctcgaggc cgtcgggatc gacgcccatc
     1501 ttttcggcga gttcgcggat tgtcggggcc gtccaggcct cgttgatctt gatgccttcc
     1561 atgatggcct tgcggttgac cgcgtcgtag ctcatgaagt tcgtgttgtc gcagaccgtg
     1621 tagccgtagc gttccggcgt gccgagcacg gcgtcgcgga tcacgtcgcg gcgttcgcct
     1681 tcgttgacga tgcggttgcc gcgcttgtcg acgtagatgg agccgttgac gttgaagttg
     1741 aggagaagct tcatcttctt gccggcggga gcacccggag tcgactggat gaagtccatg
     1801 ccgacgaggt aggcgccgac gcgcacggcg gccatagcga cttcgcccgt ggagccgggg
     1861 aggttgtcgg tgccgagccc cttgacgcgc gggtcatgca gctcgcgcag gtagcggttg
     1921 ccggagaagc cgccggcggc gagaacgacc gccttcgtgg cgcggatgaa gcgggcgggg
     1981 cccttggcgg gctggacctt gacgccgatc acgcggcccg caaactgctc ttcgcggatg
     2041 atttcggtca cgccgaggcc cgtgcggacc tgaacgccga gttccttggc ggccttcgtg
     2101 aggacggtgc cgtagccctg ccccttcgga agagcgggga tgtgcgagcg cggatagagc
     2161 gcgccgaaga tctggatgac gttgtccttg aacttcatgc cgagggactc gagccaggtg
     2221 atggcgccgt aggcgttgtc gcagaggacc ttcacctgct cgtagtagaa cttgatgg
//
LOCUS       DBMDTI010000318         2244 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10612_length_2244_cov_2.356043, whole genome shotgun sequence.
ACCESSION   DBMDTI010000318 DBMDTI010000000
VERSION     DBMDTI010000318.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10612_length_2244_cov_2.356043"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(677..1306)
                     /gene="pgsA"
                     /locus_tag="ACZPB6_10115"
     CDS             complement(677..1306)
                     /gene="pgsA"
                     /locus_tag="ACZPB6_10115"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR1184853.1"
                     /translation="MPKLKPPFKLNVPMLLTWMRIAMIPLVIGIFYLPETWMTMGTKN
                     ATAMLMFVIAAITDALDGFLARRYQGWATPMGAFLDPVADKLLVSAALVALVGLDRCD
                     MLVALVIIGREITVSALREWMAKVGASGAVKVNWFGKIKTIAQMTAIPMLLWWDPVVV
                     STSLLSGELNIAFLGRVLIIVAAVLTIYSMYVYLMAARKALTAPASDAL"
     gene            complement(1327..>2244)
                     /locus_tag="ACZPB6_10120"
     CDS             complement(1327..>2244)
                     /locus_tag="ACZPB6_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1184854.1"
                     /translation="AVMSDEERHETVEAVAEAMSAAAGRRVTVVAEPRETRRRWLEMC
                     AKGAEIALARHLQEEGSQLARLRELIDILGFEPDDGDPMNFRVECFDISHTAGEATQA
                     SCVVFSEGRMQSALYRRFNITGIEPGDDYAAMRQVLERRYRPVTRGEAVLPSVVLVDG
                     GKGQVEMARQVFTELGLDLSVIVGVAKGEGRKVGLETLIFPEIDGVRREPLVLGTMSR
                     ALMLIAEIRDEAHRFAITGMRAKRAKTRQSSRLEDLEGIGPKRRAKLLTHFGGMKQLS
                     NASAEDIARVDGISAALAAKIYAQLHGSQ"
BASE COUNT          383 a          706 c          731 g          424 t
ORIGIN      
        1 tcgcaaagcg gatgatcgtg cgcacggcct cgatgccgtg ctgccgccga gcccgcttca
       61 caagctccgc actcctcgtt ccggcctctc cctctgcatg gtcctcggag gccgtcttct
      121 cttccgacat cgtctgcatg ctatctcttc ttctccatga cgcccgtcgt tgatgtcacg
      181 agctcttgcg ctcattgtac aggccgccaa ctcccgcagg cccgcgcggc ctccgcgaca
      241 caaaagagcg gcagtcgacg cgatccttcg cccgaatggt cgacaaatga ttttctcctg
      301 caacacacgg actttgcccg caccattctc agaaaactcc aggctcttcg catcacaggg
      361 cttgaattgc cgacaatttg taaactttgc tcgacaagtc gctaaaactt gtgtatagtt
      421 cacccctatc gacggaccga ctctaagcgg ttcgtcgtct tttcggtcag acgcggctcc
      481 tgtcaggggc gcctgcgtca gcggcagtaa ggcgccgctc cgaaaaaggg ggaacgaaaa
      541 ggccggtttt cgacgaaacc ggcctttttc attttgtacg ccgcgcccgt gcgcgggagg
      601 acatgaaaaa cgccgcggac cgcggcgttt tttttgattt ccggattcgc ttccgggttc
      661 gtctcgggag tcgttctcag agtgcgtcgg acgcgggagc cgtgagagcc ttgcgggcgg
      721 ccatgaggta gacgtacatc gaatagatgg tgagcacggc ggcgacgatg atgaggacgc
      781 ggccgaggaa ggcgatgttg agctcgccgc tcaggaggct cgtgctaacg acgacgggat
      841 cccaccagag gagcatcggg atggcggtca tctgggcgat ggtcttgatc ttgccgaacc
      901 agttgacctt gacggcgccc gaggcgccga ccttggccat ccactcgcgc agggcggaga
      961 ccgtgatctc gcggccgatg atgacgaggg cgacgagcat gtcgcatcgg tcgaggccca
     1021 cgagggcaac gagcgcggcg ctcacgagga gcttgtcggc gacgggatcg aggaaggcgc
     1081 ccatgggcgt ggcccagccc tgatagcggc gggcgaggaa gccgtcaagc gcgtcggtga
     1141 tggcggcaat gacgaacatc agcatggcgg tcgcgttctt cgtccccatc gtcatccacg
     1201 tttcgggaag atagaagatg ccgatgacga gcggaatcat cgcgatgcgc atccaggtga
     1261 ggagcatcgg gacgttgagc ttgaatgggg gcttgagttt gggcatgtgc gggtccttgt
     1321 cgctttttac tggctcccgt ggagctgagc atagatttta gccgcgagcg cggcggaaat
     1381 gccgtcgact cgcgcaatat cttcggccga ggcgttcgaa agctgcttca tgccgccaaa
     1441 atgcgtgaga agcttcgcgc ggcgcttggg tccgatcccc tcgagatcct cgagccggct
     1501 cgactgacgg gtcttcgcgc gcttggcgcg catgcccgtg atggcgaagc ggtgggcctc
     1561 gtcgcggatc tcggcgatga gcatgagcgc gcggctcatg gtgccgagca cgagcggctc
     1621 gcggcgcacg ccgtcgatct cggggaagat cagggtctcg agacccacct tgcggccctc
     1681 gcccttggcg acgccgacga tgacggaaag atcgagtccg agctcagtga agacctgtct
     1741 ggccatctcg acctgcccct tgccgccgtc gacgagcacg acgctcggca gcacggcctc
     1801 gccgcgcgtc acgggcctgt agcggcgctc gagcacctgg cgcatggccg cgtagtcgtc
     1861 acctggctcg atgcccgtga tgttgaagcg cctgtagagg gcggactgca tgcggccctc
     1921 ggagaagacg acgcaggagg cctgcgtggc ctcgcccgcc gtgtgcgaga tgtcgaagca
     1981 ctcgacgcgg aagttcatcg gatcgccgtc gtcgggctcg aacccgagga tgtcgatgag
     2041 ctccctgagt cttgcgagct ggctgccttc ctcctgaagg tggcgcgcga gcgcgatctc
     2101 ggcacccttc gcgcacatct cgagccagcg cctgcgcgtc tcgcgcggct ccgccaccac
     2161 cgtgacgcgc cttccggccg cggccgacat cgcctcggcg acggcctcga ccgtctcgtg
     2221 gcgctcctcg tcggacatga cggc
//
LOCUS       DBMDTI010000319         2240 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10643_length_2240_cov_1.679757, whole genome shotgun sequence.
ACCESSION   DBMDTI010000319 DBMDTI010000000
VERSION     DBMDTI010000319.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10643_length_2240_cov_1.679757"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="ACZPB6_10125"
     CDS             <1..639
                     /locus_tag="ACZPB6_10125"
                     /inference="COORDINATES: protein motif:HMM:NF052145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-type ATPase lid domain-containing protein"
                     /protein_id="HIR1184855.1"
                     /translation="KEVTVDVRIICATNRDLRSMVVEGKFREDLYYRLNVYPIPVAPL
                     RERPEDIEPLASHFIRQIARRYGMAVLPRLSEEALDFARRWPWPGNVRELRNVMARAV
                     LSGEPVIRTLDVSTGAAPDVHPVPFAVRGLGVTGPSDVHGAQEGPADMKAEVNADMTF
                     DDMQRAYFRALLERTGGRVSGAGGAAERAGMHPNTLRSRLEKLGITLRRTVG"
     gene            771..1700
                     /locus_tag="ACZPB6_10130"
     CDS             771..1700
                     /locus_tag="ACZPB6_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1184856.1"
                     /translation="MSLFQLRQTVLLLITATIWGLSFVAQSVGMDHVGPFTFTASRMF
                     LGALVLLPFVLWNRSRLAASNPAEHARRTSPAYMKMLLLGGAACGLCLFGGESLQQFG
                     LVHDTEVGKAGFITALYIVLVPMIGLALGRRTGWLVWAAVAVAIGGLWFLCVPPEGFS
                     VRLGDLYTLLCAFVFSLHILVISHFVTKVNGVELACGQFVCGSIIASAAVLIAESPTI
                     EGLQGALLPILWAGIMSNGIAYTLQIVGQRGMNETVASLIMSLESAIAVIAGWLLLGE
                     VLSQRELFGCVLMAGAVVLAQLPAPKSRRSGKD"
     gene            complement(1717..>2240)
                     /locus_tag="ACZPB6_10135"
     CDS             complement(1717..>2240)
                     /locus_tag="ACZPB6_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR1184857.1"
                     /translation="SSATTDVDKVRWIGLFSRRTLLGSVRAIGHGKAGMTLMATAVTD
                     VSGFAVPLELYARWVSENPERERAMLLNCIAKSECQVEGVLVNDLCSVDDRVEIAVAV
                     LFRAAGITEAQLPAALLWDIPVSDIASLVHAERAMTSRAVGRMVSSGLLERSGRTFVL
                     KAVPPAARLWWGA"
BASE COUNT          350 a          736 c          773 g          381 t
ORIGIN      
        1 aaggaggtga cggtcgacgt gcgcatcatc tgcgcgacga acagggacct gaggagcatg
       61 gtcgtcgagg gaaagttccg cgaggacctc tactatcgcc tcaacgtcta tccgattccc
      121 gtcgcgcccc tgagggagag gcccgaggac atcgagccgc tcgcctcgca cttcatcagg
      181 cagatcgcaa gacgatacgg catggccgtg ctcccgaggc tctcggagga ggcgctcgac
      241 ttcgcgaggc gctggccgtg gccgggcaac gtgcgcgaat tgagaaacgt catggcccga
      301 gccgtcctga gcggcgagcc cgtcatcagg acgcttgacg tgagcacggg cgccgcgccc
      361 gacgtgcacc ccgtcccctt tgccgtgcgc ggactcggcg tcacgggccc atcggacgtg
      421 cacggcgcgc aggaagggcc tgcggacatg aaggcggagg tgaatgccga catgaccttc
      481 gacgacatgc agcgtgccta cttcagggcg cttcttgagc gcacgggcgg gcgcgtgagc
      541 ggtgcgggcg gcgcggccga aagggcgggc atgcatccca acacgctcag gagcaggctc
      601 gagaagctcg ggatcacgct ccggaggacg gtagggtaag gtgcgcgaat gcggccccgc
      661 gcgggcgttt ccggggcggg atcgtgcccg cggcgcgcgt cggggcctct gatagaatgg
      721 gcggattcag ccctgaagaa aaacaacatt ccaagcagaa cagacccgtc atgtcgctct
      781 ttcagcttcg ccaaaccgtt cttctcctca tcacggccac gatctggggc ctgagcttcg
      841 tcgcgcagtc ggtcgggatg gaccacgtcg gtcccttcac cttcacggcg tcgcgcatgt
      901 tccttggcgc cctcgtgctg ctgcccttcg tcctgtggaa ccgctcgagg ctcgccgcct
      961 cgaatcccgc ggagcacgcg cgccgcacct cgcccgccta catgaagatg ctccttctcg
     1021 ggggcgcggc ctgcggcctg tgcctcttcg gcggcgagtc gcttcagcag ttcgggctcg
     1081 tgcacgacac cgaggtcggc aaggcgggct tcatcacggc gctctacatc gtgctcgtgc
     1141 cgatgatcgg gcttgcgctc ggccgcagga cgggctggct cgtctgggcg gccgtggcgg
     1201 tcgcgatcgg cgggctctgg tttctctgcg tgccgcctga aggcttttcg gtgaggctcg
     1261 gggatctcta cacgctgctg tgcgccttcg tcttctcgct tcacatcctc gtcatctcgc
     1321 acttcgtcac gaaggtcaac ggcgtcgagc tcgcctgcgg acagttcgtc tgcggctcga
     1381 tcatcgcctc ggcggccgtg ctgatcgcgg agtccccgac cattgaaggc cttcaggggg
     1441 cgctccttcc catcctctgg gcgggcatca tgtccaacgg catcgcctac acgctgcaga
     1501 tcgtgggcca gcgcggcatg aacgagacgg tcgcgagcct catcatgagc ctcgagagcg
     1561 ccatcgccgt gatcgcgggc tggctgctgc tcggcgaagt gctctcccag agggagctct
     1621 tcggctgcgt gctgatggcg ggcgccgtcg tactcgcgca gcttcctgcg cccaagtccc
     1681 gccgttccgg gaaggactga ggaccagcgg gggcgcttag gcgccccacc agaggcgggc
     1741 tgcgggcggc acggccttga gcacgaaggt cctgcccgaa cgctcgagca gaccgcttga
     1801 gaccatcctg cccacggctc ggctcgtcat ggcgcgctcg gcgtgcacga ggctcgcgat
     1861 gtcggagacg ggaatgtccc acagcagcgc ggcgggcagc tgcgcctcgg tgatgcctgc
     1921 ggcgcgaaag agcaccgcaa cggcgatctc gacgcggtcg tcgacggagc agaggtcgtt
     1981 gacgagaacg ccctcaacct gacactcgga cttggcgatg cagttgagaa gcatggcgcg
     2041 ctcgcgttcg ggattctccg agacccagcg ggcgtagagc tcgaggggga cggcgaagcc
     2101 gctcacgtcg gtgacggccg tggccatgag agtcatgcca gccttgccgt gaccgatggc
     2161 gcgcacgctg ccgagcagcg tcctgcgaga gaagaggccg atccatcgga ccttgtcgac
     2221 gtccgtggtc gcggagcttg
//
LOCUS       DBMDTI010000320         2234 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10677_length_2234_cov_2.442100, whole genome shotgun sequence.
ACCESSION   DBMDTI010000320 DBMDTI010000000
VERSION     DBMDTI010000320.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10677_length_2234_cov_2.442100"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /locus_tag="ACZPB6_10140"
     CDS             complement(<1..1744)
                     /locus_tag="ACZPB6_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR1184858.1"
                     /translation="MLTVIKKDNTTEEFIDEKIVDAVKKAAFRCDTLIDDEGLARIVS
                     EVRSRLEGRDEVSVLELHELVIAVLSAQQHKQVAEAYAEYRYYKTTYARTFEQLRQDA
                     DDVLRLGDRENANYDSSLVSTKGSLIKGYLTKSLYKQFYLSGKEKALIERGDIYIHDL
                     RDMILGCINCCLFDIGHVLKGGFEMSNVQYSEPKSVLSALQVIGDITLVATAQQFGGF
                     TLPELDKVLLPYVEKSLAAARAKYRDLGLDEDTVERVASRDVVRELEQGFQSLELKLN
                     TVPCSRGDFAFTTITFGQWNPELFTEEEREWLMTISRIMLQVRRNGHGKDGKPVVFPK
                     LVYLYDERQIEADKWSAQLFDEAVRTSSECMYPDYLSLSSENGSVSEIFQQYGAITSP
                     MGCRAFLSLWCNEEGRAVTVGRCNIGAVSLNLPIILKLAQLRHPDTWRNDFWRILDER
                     LEFIRSFFKKRYDIIRNQKCSSNPLAFTQGGFYEGTKSPDDRVGDLVRYMTASFGITA
                     LDEATYLWSGRRLVEEGGDFSASVLRHLKEKLAQFKKEDGHLYAIYGTPAESLCATQA
                     RQYDRFCRDEGVENV"
BASE COUNT          438 a          640 c          714 g          442 t
ORIGIN      
        1 acacgttctc gacgccttcg tcgcggcaga agcggtcgta ctgacgggcc tgcgtggcgc
       61 agagcgactc ggccggcgtg ccgtagatgg cgtagaggtg gccgtcctcc ttcttgaact
      121 gcgcgagctt ttccttgagg tggcgaagca cggaggcgga gaagtcgccg ccctcttcga
      181 cgaggcgcct gccgctccag agataggtgg cttcgtcaag ggccgtgatg ccgaacgagg
      241 ccgtcatgta gcgcacgagg tcgcccacgc ggtcgtcggg gctcttcgtg ccctcgtaga
      301 agccgccctg cgtgaaggcg agcgggttgc tcgagcactt ctggttgcgg atgatgtcgt
      361 agcgcttctt gaagaaggag cggatgaatt cgagacgctc gtcgagaatg cgccagaagt
      421 cattgcgcca ggtgtcggga tgacggagct gggcgagctt gaggatgatc ggcaggttga
      481 gcgagacggc gccgatgttg cagcgcccga ccgtgacggc cctgccctct tcattgcacc
      541 agagcgagag gaaggcgcgg caacccatgg gcgacgtgat ggcgccgtac tgctggaaga
      601 tctcggagac gctgccgttc tcggacgaaa ggctcaggta gtccgggtac atgcactcgg
      661 aggaggtcct gacggcctcg tcgaagagct gggcggacca cttgtcggcc tcgatctggc
      721 gctcgtcata gaggtagaca agcttcggga agacgacggg cttgccgtcc ttgccgtggc
      781 cgttgcggcg gacctggagc atgatgcggc tgatcgtcat gagccactcg cgctcctcct
      841 cggtgaagag ctcgggattc cactgaccga aagtgatcgt cgtgaaggcg aaatcgccgc
      901 ggctgcacgg cacggtgttg agcttcaatt cgagggactg gaagccctgc tcgagctcgc
      961 gcacgacgtc gcgcgaggcg acgcgctcga cggtatcctc gtcaaggccg aggtcgcggt
     1021 acttggcgcg ggcggcggcg aggctcttct cgacataggg caggagcacc ttgtcaagct
     1081 cgggcagcgt gaagccgccg aactgctggg cggtcgccac aagcgtgatg tcaccgatca
     1141 cctgaagagc ggaaagcacg gacttgggct cggaatactg gacgttcgac atctcgaagc
     1201 cgcccttgag gacgtggccg atgtcgaaga ggcagcagtt gatgcagccg aggatcatgt
     1261 cgcgcaggtc gtggatgtag atgtcgccgc gctcgatcag ggccttctcc ttgcccgaaa
     1321 gatagaactg cttgtagagg ctcttcgtga ggtagccctt gatcagggag cccttcgtgg
     1381 agacgagcga gctgtcgtag ttagcgttct cgcggtcgcc gaggcgcagc acgtcgtcgg
     1441 catcctgacg gagctgctcg aacgtgcggg cgtacgtcgt tttgtagtag cggtattcgg
     1501 cataggcctc cgccacctgc ttgtgctgct gggcggagag aacggcgatg acaagctcgt
     1561 gaagctcaag cacggacacc tcgtcgcggc cctcaaggcg cgaacggacc tcactgacga
     1621 tgcgggcgag cccctcgtcg tcgatgagcg tgtcgcagcg gaaggccgcc ttcttcaccg
     1681 cgtcaacgat cttctcgtca atgaattctt cggtcgtgtt gtccttcttg ataaccgtca
     1741 gcatgtcgtc gtccttcttt taatccttct caacagcggc cgtcattgaa accaacggac
     1801 ggacccgaaa aaacgaactg ccgccccatg ggcggcaggg acttaatctt atggaaaagc
     1861 cccgagaatc tcaacactct tttcttcgac aaacggatgt ttgcggtatt gacaaacctg
     1921 cgcaaaagtg cttcctcaaa acatccccgg cggtccggaa cgcctccccg catccgctcc
     1981 tccttagagg gaggcgcgtt tgaagtccct cccccgccgg tccgaaccgc cttttttcat
     2041 ccattctttt tttctatgag aagcatcggc accatacaat cggacgccga aaaagtcact
     2101 acatcatgtg gtttgacaac accacaacca caacatcttg tgttttgcac agatttcact
     2161 ccagagaaat gacgccccga acaacggtcg aaagcaatgg gggagagggt tttacaagcg
     2221 ggtgaaggag gagg
//
LOCUS       DBMDTI010000321         2227 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10727_length_2227_cov_2.493885, whole genome shotgun sequence.
ACCESSION   DBMDTI010000321 DBMDTI010000000
VERSION     DBMDTI010000321.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10727_length_2227_cov_2.493885"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            81..1340
                     /locus_tag="ACZPB6_10145"
     CDS             81..1340
                     /locus_tag="ACZPB6_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR1184859.1"
                     /translation="MHQNFIRAAAAAAVAATFSSAAVAGGFQLTEQSALGLGRAYAGM
                     GVDGTDVSGLYYNPATMTLHTGTQAQFGFVGVGLNLDYNSKKGDSIHDENGRKHAEAV
                     PNLFISHQINDVTWIGFGFTVPFGLSTEYGEDWEQSGEGTEAKIAVYDFNPSFAWKLS
                     DKVSLGAGISYQYVKADLGFNWTHHPLLGKGTPGMAYTKVSGDAWGWNVGLMWTPVES
                     VRIGASYRSAVGHTVNGTLNTDNHIKVPDGGNIVALPTSMNAQVSMDAPAWAMLSAAW
                     DVNDWLSLYGTMRWADWSSFKSLTIETQHQDFESKKNWKDTYLYSVGYDARINSFWTL
                     RGGIAYETSTIDDKYTRTGTIPDADRWWFAIGSSFHWTKDFQTDVGFAHLHGVHERSL
                     YNSKHEEIGKFRKLDAYIIGIQGQYRF"
     gene            complement(1344..1841)
                     /locus_tag="ACZPB6_10150"
     CDS             complement(1344..1841)
                     /locus_tag="ACZPB6_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184860.1"
                     /translation="MLRAHRCRRYAARLKELIRCTPVDPLLLTEGEVTDLACLHADST
                     RPTAGECLLHDDRSPVLLLSSLHPAPETLWDEDLALLTLLLKHFGLRPSVETGLALYT
                     AVLCRSAGFTDCPEFEGFERPALPEGADLGTLLVDFLYPESKNPGVWETPGFGVQDAA
                     EFAAG"
     gene            2002..>2227
                     /locus_tag="ACZPB6_10155"
     CDS             2002..>2227
                     /locus_tag="ACZPB6_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184861.1"
                     /translation="MSNSAIPDLISEDIFRFGPGDRYALPIGTAAPDALALSQMAEKC
                     RTRHERLLVICADPLDAVRLGQEIPWFSPEL"
BASE COUNT          449 a          753 c          636 g          389 t
ORIGIN      
        1 cacctcattc gggaaaacac gcataaaaag cccaaaaaac gacatgtgtc gcgcactcca
       61 atccatacca ggagcagatc ttgcatcaga atttcatccg cgccgccgct gccgccgccg
      121 tggccgccac cttctcctcc gccgccgttg ccggcggctt ccagcttacg gagcagtccg
      181 ctctcggtct cggccgcgcc tacgccggca tgggtgtcga cggcaccgac gtctcgggcc
      241 tctactacaa ccccgccacg atgacgcttc acacgggcac gcaggctcag ttcggcttcg
      301 tcggcgtcgg cctcaacctc gactacaaca gcaagaaagg cgactccatc catgacgaaa
      361 acggccgcaa gcacgccgaa gccgtcccga acctcttcat ttcgcaccag atcaatgacg
      421 tgacctggat cggcttcggc ttcacggtgc cgttcggcct ctccaccgaa tacggtgaag
      481 actgggagca gtccggcgaa ggcacggaag ccaagatcgc ggtctatgac ttcaacccgt
      541 cctttgcctg gaagctctcc gacaaggtct cgctcggtgc cggcatttcc taccagtacg
      601 tgaaggccga cctcggcttc aattggaccc atcatcccct gcttggcaag ggcactccgg
      661 gcatggccta cacgaaggtc tccggcgacg cctggggttg gaacgtcggc ttgatgtgga
      721 cgcctgttga aagcgtccgc atcggtgcct cctaccgctc cgccgtcggg cacacggtca
      781 acggcacgct caataccgac aaccacatca aggtccctga cggaggaaat atcgtcgccc
      841 tgccgacttc catgaacgct caggtctcca tggacgcccc cgcctgggcc atgctctccg
      901 ccgcttggga cgtcaacgac tggctgagcc tctacggcac catgcgctgg gccgactggt
      961 cgagcttcaa atccctgacg attgaaaccc agcaccagga ctttgagtcc aagaagaact
     1021 ggaaggacac gtacctctac tccgtcggct acgacgcccg catcaacagc ttctggacgc
     1081 tgcgcggcgg catcgcctac gaaacgtcca cgatcgacga caagtacacg cgtaccggca
     1141 cgattcccga cgccgaccgc tggtggttcg ccatcggctc ctccttccac tggacgaagg
     1201 acttccagac ggacgtgggc ttcgctcacc tgcacggcgt gcatgaacgc agcctctaca
     1261 actccaagca tgaggaaatc ggcaagttcc gcaagcttga tgcctacatc atcggcatcc
     1321 agggtcagta ccgcttctaa ggtctagccc gcggcgaact ccgccgcatc ctgcactccg
     1381 aatcccggcg tctcccagac gccgggattt ttgctttcgg gatagaggaa atcgacaagc
     1441 agcgtgccga ggtcggcgcc ctcgggcagt gcggggcgct cgaaaccctc gaattcaggg
     1501 cagtcggtga agcccgcgct tcggcagagg acggcggtgt agagcgcaag ccccgtctcc
     1561 acgctcggac gaaggccgaa gtgcttaaga agaagcgtga gaagcgcgag gtcctcgtcc
     1621 catagagtct cgggagcggg atgaagggag gagagaagga ggacgggaga cctgtcgtca
     1681 tgaaggaggc attcgccggc cgtcgggcgc gtgctgtcgg cgtgaaggca cgcaaggtcc
     1741 gtcacctctc cctcggtgag aagaagcgga tccacgggcg tgcagcggat gagttccttc
     1801 aagcgcgccg cataacgtct gcatcgatga gctctcagca tggtcgggcc tccatcggcc
     1861 gctcgcggat cgggcggcct tcacctaatc ctacggcata tcggccgaag gtgtcgcccc
     1921 tcgcctgtcg accgccctct gagagtcctc cggtttgtgc tatcgtcact tccgactcaa
     1981 gacacaaaac ggagcccaca catgtccaat agcgcaatcc cagacctgat ttccgaagac
     2041 atcttccgtt tcgggcccgg cgacaggtac gcgctgccca tcggcacggc ggcacccgac
     2101 gcgctcgcgc tctcgcagat ggccgagaag tgccgcacaa ggcatgaaag actcctcgtc
     2161 atctgcgccg atccgctcga cgccgtgagg ctcgggcagg agatcccctg gttcagtccc
     2221 gaactcg
//
LOCUS       DBMDTI010000322         2210 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10844_length_2210_cov_2.277383, whole genome shotgun sequence.
ACCESSION   DBMDTI010000322 DBMDTI010000000
VERSION     DBMDTI010000322.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10844_length_2210_cov_2.277383"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="ACZPB6_10160"
     CDS             <1..476
                     /locus_tag="ACZPB6_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR1184862.1"
                     /translation="AEIVGYGLSGDAFHITTPSTDGPARCMRMALRHAGIAPEDIQYL
                     NAHGTSTNVGDVNEVKAIKEVFGEHAGKLVVNSTKSMTGHLLGGAGGVESVFTIKALE
                     EQVSPPTINVFEQDPECDIDCCPNEARRMEINYAMKNSFGFGGTNSTVIYKRWTE"
     gene            635..1915
                     /locus_tag="ACZPB6_10165"
     CDS             635..1915
                     /locus_tag="ACZPB6_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1184863.1"
                     /translation="MQDCARYVEEMTRLRREIHRRPEEGWTEFETQWKVFRALEALGW
                     TIRMGLDVIRPEAVMGRNAELVEKAMVRAAEHGVPESFLKATGGYTGLTANFDTGREG
                     PVTGFRFDMDCVLVEELHDASHLPAAEGFDSEIPGHMHACGHDAHTATGVTLAHWITD
                     HKDELKGRFRLIFQPAEEGTRGAAPMAAAGVVDDLNWFFGAHVGCNCRIGEASVVKKG
                     FLATTKIDIEFTGVPSHAGSDPEKGRSALMAAAAAAVMMQGIPRHGEGDTRIAVGRLV
                     AGEGRNVTPVHAFMQCETRGSTQEINEYMFESVRRIVEGCAASYGVECRVTKAGEATN
                     FEATPAACDLAVEACAEVFGRDHVTSLETAGGSEDCSILARRAIEHGAEATFLFYGCN
                     HHGHHRADFEIQDTQSLPAALAVLSGICRRTNGL"
BASE COUNT          417 a          739 c          698 g          356 t
ORIGIN      
        1 acgccgaaat cgtcggctac ggcctctcgg gcgacgcctt ccacatcaca accccgtcga
       61 ccgacggtcc cgcccgctgc atgcgcatgg cgctccgcca cgccggcatc gcccccgagg
      121 acatccagta cctcaacgcg cacggcacgt ccacaaacgt cggcgacgtc aacgaagtga
      181 aggccatcaa ggaagtcttc ggcgaacacg ccgggaagct cgtcgtcaac tccacgaagt
      241 ccatgacggg ccaccttctc ggcggcgccg gcggcgtcga gtccgtcttc acgatcaagg
      301 cgctcgagga gcaggtctcg cccccgacga tcaacgtctt cgagcaggat cccgaatgcg
      361 acatcgactg ctgtcccaac gaggcccgca ggatggagat caactacgcc atgaagaact
      421 ccttcggctt cggcggcacg aactccacgg tgatctacaa gcgttggact gaataaggcg
      481 gcttgttcct gcgcagtccg caggaccatc cgcatgcgca aaagggcact ctcctcacgt
      541 tgaggcgggt gcctttttta tgccactcct cccgcggtct caaaagagga ggaaaatgtc
      601 tgcatgacat tctttccaat caaaggagaa cgacatgcag gactgcgcca ggtacgtcga
      661 ggagatgacg aggcttcgcc gtgaaatcca ccgccgcccc gaagagggct ggaccgaatt
      721 cgagacccag tggaaggtct tccgtgcgct tgaggcgctc ggctggacga tccgcatggg
      781 gctggacgtg atcaggcccg aggccgtcat gggccgcaat gcggagctcg tcgagaaggc
      841 gatggtccga gcggccgaac acggcgtgcc tgaatccttc ctcaaggcca cgggcggcta
      901 cacggggctc acggccaact ttgacacggg ccgcgaaggc cccgtcacgg gctttcgctt
      961 cgacatggac tgcgtactcg tcgaggagct tcacgacgcc tcgcacctgc ccgcggccga
     1021 agggttcgac tccgagatcc cgggacacat gcacgcctgc ggccatgacg cccatacggc
     1081 gacgggcgtg acgctcgccc attggatcac ggatcacaag gacgagctca aggggcgctt
     1141 ccgcctcatc ttccagcccg ccgaagaagg gacgcgcggc gcggcgccca tggccgccgc
     1201 aggcgtcgtg gacgatctca actggttctt cggcgcccat gtgggctgca actgcaggat
     1261 cggcgaggcg tccgtcgtga agaagggctt cctggccacg accaagatcg acatcgagtt
     1321 cacgggcgtg ccctcgcatg cgggttcgga tcccgaaaag ggcaggagcg cgctcatggc
     1381 cgcggcggcc gccgccgtga tgatgcaggg cattccgcgc cacggcgagg gcgacacgcg
     1441 catcgccgtc ggcaggctcg tggcgggcga gggcaggaac gtcacgcccg tgcacgcctt
     1501 catgcagtgc gagacccgcg gctctacgca ggagatcaac gaatacatgt tcgagagcgt
     1561 caggcgcatc gtcgagggct gcgccgcctc ttacggcgtc gaatgtcgcg tgacgaaggc
     1621 gggcgaggcc acgaacttcg aggcgacgcc tgccgcctgc gacctggcgg tcgaggcctg
     1681 cgcggaggtg ttcgggcgcg atcacgtgac cagtcttgag actgcgggcg gcagcgagga
     1741 ctgctcgatt cttgcgcgcc gcgccatcga gcacggcgcc gaggcgacct tcctcttcta
     1801 cggctgcaac catcacggcc atcatcgcgc cgacttcgag atccaggaca cgcagagcct
     1861 gcccgccgca cttgccgtgc tttcgggcat ctgccgccgc accaacggcc tctgatcccg
     1921 atatccgatc acgggagctc cgcctgcggg cggctcccgt ccggcgccgc gaggcttccc
     1981 gcacgtcctt cgtgcaggaa cccgcggcgc ttccttattg gcaaagcgga cggcattcgc
     2041 ccgaatccgc tgcggtgcaa aagattcggg gaaattcaat gtgtaatggc gcctgaatgg
     2101 tacaataata ggtcctagaa aggcccgatg acggccatgt tttgcaagag ccgcccgttc
     2161 ccgtcgagca ttgcgctccg gccggcggcc cgtacaaatt ccgacccatt
//
LOCUS       DBMDTI010000323         2210 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10845_length_2210_cov_2.198672, whole genome shotgun sequence.
ACCESSION   DBMDTI010000323 DBMDTI010000000
VERSION     DBMDTI010000323.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10845_length_2210_cov_2.198672"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="ACZPB6_10170"
     CDS             complement(<1..394)
                     /locus_tag="ACZPB6_10170"
                     /inference="COORDINATES: protein motif:HMM:NF025711.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HIR1184864.1"
                     /translation="MADHHDEEAACPKCGGTITFTLWENLVAEKYPEAAEKLMRGELF
                     AYECPHCHATGNGYYPLRFTDMTSKVMVDYPATTDLRVAGEMVNEFREKQKLFAETGF
                     PLDYDYRVVIDPNALVEKAQIFAAGLDDR"
     gene            complement(488..1141)
                     /locus_tag="ACZPB6_10175"
     CDS             complement(488..1141)
                     /locus_tag="ACZPB6_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1184865.1"
                     /translation="MARRQIRTLTPTELARVQQGALVRIVDDDDSLRDALRFVLETEG
                     WRVADYRSANDFFRGDAPSVPGCVVMDVRMPGLTGIEAQAVMNERGFSLPVIFLTGHG
                     DIDMAVMALHEGAADFIQKPVDNERLLAVIASTAFESLSGAGAVLDGETAKARCAELT
                     NRERDIARLVAEGLTNRLIGERLSIAVRTVEVHRASALRKLGVRTPEEVRAVLEAGA"
     gene            complement(1159..>2210)
                     /locus_tag="ACZPB6_10180"
     CDS             complement(1159..>2210)
                     /locus_tag="ACZPB6_10180"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1184866.1"
                     /translation="NGIYWGVATDYSSVDKLFRETRTGPYAYLNTWSLKRFIAEYWHW
                     LMLAGGLVAGLTLHSVRVTQLVHKRTAALRQSLAELQVLKEEAQGAAQRIERLERAGV
                     VAQLSVIFAHEMRQPLGAISLYSFGLRRMLGNGSTDTARMTDVIDRLDRQTERANEIV
                     ARVRSYAKAEQPTRVLVSLREQVDRAAADLKTTGRYSTALRVIVTDEPVVTADPLEME
                     LVALNLMKNALEASDAAGGAGEVVVTLFEEDERAIITVADDGGGTRETVERLNRGLGS
                     TKAEGLGLGLSIVRGILEAYGGRLAFSARRQGGLVARVTVPVREVSGEPSPLDDDSLA
                     TGDNAVRDNRDEKAS"
BASE COUNT          335 a          758 c          693 g          424 t
ORIGIN      
        1 cgcggtcgtc gagcccggcc gcgaagatct gggccttttc gacgagggcg ttcgggtcga
       61 tcacgacgcg gtagtcgtag tcaagcggga agcccgtctc ggcaaagagc ttctgcttct
      121 ccctgaattc gttcaccatc tcgcccgcga cgcgcaggtc ggtcgtggcg ggatagtcga
      181 ccatgacctt tgaggtcatg tcggtgaagc gaagcggata gtagccgttg cccgtcgcat
      241 ggcagtgcgg gcattcatag gcgaagagct cgcccctcat gagcttttcc gccgcttcgg
      301 ggtatttctc tgcgacgaga ttctcccaga gcgtgaaggt gatcgtgccg ccgcacttcg
      361 gacatgcggc ttcctcgtcg tgatggtcgg ccacggcgca actccttaga gtgatgttga
      421 tggtgcgcag agtctagcgg agcttcgttg cgcctgcctt acgtccgatc tcgtccttcc
      481 ttgcccgtca ggcgcccgcc tcgagcacgg ccctcacctc ctcgggcgtc ctgacgccga
      541 gctttctcag agccgacgcc ctgtgcacct cgaccgtacg cacggcgatc gagagtcttt
      601 cgccgatgag cctgttcgtg agtccctcgg ccacgagcct cgcgatgtcg cgctccctgt
      661 tcgtgagctc cgcgcagcgc gccttcgccg tctcgccgtc gagcaccgcg cccgcgccgc
      721 tcaggctctc aaaggccgtg gacgcgatca cggccagaag ccgctcgttg tcgacgggct
      781 tctggatgaa gtccgctgcg ccttcatgga gcgccatcac ggccatgtcg atgtcgccat
      841 gccccgtgag aaagatgacg ggcaggctga agccgcgctc gttcatgacg gcctgcgcct
      901 caatgcccgt cagtcccggc atacgcacgt ccatgacgac gcagcccggc accgatggtg
      961 cgtcccctct gaaaaagtcg ttcgccgacc tgtagtcggc cacgcgccag ccctcggtct
     1021 cgagcacgaa gcgaagcgcg tcgcgaagcg aatcgtcgtc atccacaatg cgcacgagcg
     1081 ctccctgctg cacccgcgcg agctccgtgg gcgtgagcgt tctgatctgt cgtcttgcca
     1141 tgcttgcgtc tcccggcctc aggaggcctt ctcgtccctg ttgtctcgaa cagcattatc
     1201 gcctgtcgcc aaggaatcgt catccaaagg cgacggctct ccggagacct ccctcacggg
     1261 cacggtcacg cgggccacga gcccgccctg acggcgtgcg ctgaaggcga gcctgccgcc
     1321 gtaggcctcg aggatgccgc gcacaatcga aagcccgagt ccgagccctt cggctttcgt
     1381 ggagccgagt cctctgttga ggcgctcgac ggtctcgcgc gtcccgccgc cgtcgtcggc
     1441 cacggtgatg atcgcgcgtt cgtcctcctc gaagagcgtc accacgacct cgcccgcgcc
     1501 gcccgcggcg tcggacgcct cgagcgcgtt cttcatgagg ttgagcgcaa caagctccat
     1561 ctcgagagga tcggccgtca cgacgggctc gtcggtgacg atgaccctga gcgccgtgct
     1621 gtagcgtccc gtggtcttga ggtcggccgc cgccctgtcg acctgctcgc gcaggcttac
     1681 gaggacgcgg gtgggctgct cggccttcgc gtagcttctc acgcgcgcga cgatctcgtt
     1741 ggcgcgttcg gtctgccgat ccagcctgtc gataacgtcc gtcatcctgg cggtgtcggt
     1801 cgagccgttg cctagcatcc ttctcaggcc gaagctgtag agcgagatgg caccgagcgg
     1861 ctgcctcatc tcgtgcgcga aaatgacgga aagttgcgcc accacgccgg cacgctcaag
     1921 gcgttcgatg cgctgggccg ccccctgggc ctcctccttg aggacctgca gctcggcaag
     1981 ggactgcctc agggcggccg tccgcttgtg cacgagctgc gtcacacgca cgctgtgcaa
     2041 agtcaggccc gcgacaaggc cccccgcgag catgagccag tgccagtact cggcgatgaa
     2101 gcgcttcagg ctccaggtgt tgagataggc atagggcccc gttcttgtct cgcgaaagag
     2161 cttgtcgacc gagctgtagt cggtcgccac gccccagtag atgccgttgg
//
LOCUS       DBMDTI010000324         2203 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10889_length_2203_cov_1.687916, whole genome shotgun sequence.
ACCESSION   DBMDTI010000324 DBMDTI010000000
VERSION     DBMDTI010000324.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10889_length_2203_cov_1.687916"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="ACZPB6_10185"
     CDS             <1..1391
                     /locus_tag="ACZPB6_10185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184867.1"
                     /translation="SEGNMSAMDPEPEKLLPMTQAVRDIIAKYTNPKVACSPEAAELQ
                     KTVLKQLAEHDTAGKKYIFDSPELFALQTIVGGNCKNDAKLVLTMTRNATAAMKWLDA
                     QSEMTWFHVPRSWVDVGIGGLYPRGQWPRQADGKKPISTYDAYIRPLAAKVEVAGNPI
                     YKNMKVVSVERDASGRVTGVKAVDRKGVERVFSGRSVVLAAGGYGANLQMVKKYNDIS
                     VTVTSNSPGTTGEVLEEAVAAGAGLTGMEWIQIHPHGNPKNGELESAIAARPSDTPYV
                     NRDGLRFVDETGRRDEISYGILKQPGQVAFSIYDQRTIEEKKVEEKKIESAIAHGYAF
                     KADTLEDLARQAGIDWKNFEKTMKAYNEASVSQNTKSLPVPKILIGNPVVKAPFYAVP
                     ITTTIHHTMGGLRINEKTQVLDKNGNVIPGLYAAGEITGGIHGGNRLGRNALTDLLVF
                     GHIAGQEVAKVK"
     gene            complement(1542..>2203)
                     /locus_tag="ACZPB6_10190"
     CDS             complement(1542..>2203)
                     /locus_tag="ACZPB6_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1184868.1"
                     /translation="FSILDPECCRSLGAAQVAASLADITAHVLEQYMTEPGQSRPMDR
                     WAEGLLLTLVDIAPALRTPEPDYDVLADYMLTATLGLNDMICMGVNQDWATHAIGHEI
                     TALTGMAHGATLAIVFPALLRVLIDTKEAKLVQMGERVFDVHYGTDRERALETIVRLE
                     AFFASLGLPTRLDEAGVGEDVEREIVRRFEERNWKLGEHKNITPAQIAAILASARPTK
                     A"
BASE COUNT          425 a          711 c          717 g          350 t
ORIGIN      
        1 gctccgaagg caacatgtcc gccatggacc ccgaacccga aaagcttctg ccgatgacgc
       61 aggccgtgcg cgacatcatc gccaagtaca cgaacccgaa ggttgcctgc agtcccgagg
      121 ccgccgagct tcagaagacg gttctcaagc agctcgccga gcatgacacc gctggcaaga
      181 agtacatctt cgactctccc gagctctttg cgcttcagac gatcgtgggc ggcaactgca
      241 agaacgacgc caagctcgtt ctcacgatga cccgcaacgc cacggccgcc atgaagtggc
      301 ttgacgccca gtccgaaatg acgtggttcc acgtgccgcg cagctgggtc gacgtcggca
      361 tcggcggcct ctacccgcgc ggccagtggc cgcgtcaggc cgacggcaag aagccgatct
      421 ccacgtacga cgcctacatc cgtccgctcg ccgccaaggt cgaggttgcg ggcaacccga
      481 tctacaagaa catgaaggtt gtgagcgtcg agcgtgacgc ctccggccgc gtcacgggcg
      541 tgaaggccgt cgaccgcaag ggcgtcgagc gcgtcttctc cggccgcagc gtggtcctcg
      601 ccgcgggcgg ctacggcgcc aacctgcaaa tggtcaagaa gtacaacgac atctccgtca
      661 ccgtgacctc gaactctccg ggcaccaccg gcgaagtgct tgaggaagcc gtcgccgcag
      721 gcgccggtct cacgggcatg gagtggatcc agatccatcc gcacggcaat ccgaagaacg
      781 gcgagcttga gtccgccatc gccgcccgtc cgtccgacac gccgtacgtc aaccgcgacg
      841 gcctgcgctt cgtcgacgaa acgggtcgcc gcgacgagat ctcttacggc atcctgaagc
      901 agcccggtca ggtcgccttc tcgatctacg accagcgcac gatcgaggag aagaaggtcg
      961 aggagaagaa gatcgagtcc gccatcgccc acggctatgc cttcaaggcc gacacgcttg
     1021 aagacctcgc ccgccaggcc ggcatcgact ggaagaactt cgagaagacc atgaaggcct
     1081 acaacgaggc ctccgtgtcc cagaacacga agtctcttcc cgtgccgaag atcctcatcg
     1141 gcaatccggt cgtgaaggcg ccgttctacg ccgtgccgat cacgacgacc atccaccaca
     1201 cgatgggcgg tcttcgcatc aacgagaaga cccaggtcct cgacaagaac ggcaacgtga
     1261 tcccgggcct ctacgccgca ggtgagatca cgggcggcat ccacggcggc aatcgcctcg
     1321 gccgcaacgc cctgaccgac cttctggtct tcggccacat cgccggccag gaagtcgcca
     1381 aagtcaagta aggccgaatg agagcttcgc cactcgtccc tcgcgggcgg cacaacaaag
     1441 aaggaaaggg cgccagctgt gcaaaagact gctggcgccc ttcttcttgc tgttctccgc
     1501 gcagccgtcc acggggacgc gcgtgcagag gattttcagc ctcaggcctt tgtggggcgg
     1561 gccgaggcga ggatcgcggc gatctgggcg ggcgtgatgt tcttgtgctc gccgagcttc
     1621 cagttgcgtt cctcgaagcg gcggacgatc tcgcgttcga cgtcttcacc gacgcctgcc
     1681 tcgtcaaggc gggtcggcag accgagggag gcgaagaagg cctcgagcct cacgatggtt
     1741 tcgagggcgc gctcgcggtc ggtgccgtaa tgcacgtcga agacgcgttc gcccatctgc
     1801 acgagcttgg cttccttcgt gtcgatgagg acgcgaagga gagcggggaa gacgatggcg
     1861 agcgtggcgc cgtgggccat gcccgtgagg gcggtgattt cgtggccgat ggcgtgggtg
     1921 gcccagtcct gattcacgcc catgcagatc atgtcgttga ggccgagcgt agccgtgagc
     1981 atgtagtcgg cgagcacgtc gtagtcgggt tcgggcgtac gtagcgcggg cgcgatgtcg
     2041 acgagcgtga gaaggagccc ttcggcccag cggtccatcg gacgggactg gccgggttcg
     2101 gtcatgtact gctcgagcac gtgcgcggtg atgtcggcga gggacgcggc gacctgggcg
     2161 gcgccgagcg agcggcagca ctcggggtcg agaatcgaga agc
//
LOCUS       DBMDTI010000325         2201 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10901_length_2201_cov_2.620000, whole genome shotgun sequence.
ACCESSION   DBMDTI010000325 DBMDTI010000000
VERSION     DBMDTI010000325.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10901_length_2201_cov_2.620000"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="ACZPB6_10195"
     CDS             complement(<1..321)
                     /locus_tag="ACZPB6_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HIR1184869.1"
                     /translation="MSANLTKTAAAVIAAMVCAEGASAFVISDIRVEGLMRTEPGTVF
                     SHLPFRTGDEYTPDMGTRAIHSLYRSGLFKDVSLSQDGDVLVVHIVERPAVATIETHG
                     IKAFD"
     gene            complement(546..1883)
                     /gene="rseP"
                     /locus_tag="ACZPB6_10200"
     CDS             complement(546..1883)
                     /gene="rseP"
                     /locus_tag="ACZPB6_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR1184870.1"
                     /translation="MSFLLGLAGFLLTIGIIVIVHEGGHFFTAKFFNIKVTRFSFGMG
                     PVLWRRRKGETEYCFSALPLGGYVRMADEADADLTEDDRRRLYTRQARWKRGLVLFAG
                     PATNFVLAFFLYVMVGAIGTPDFAAVMGTPPAGTQAASENVRQGDRVLSVNGAPVLGI
                     TDMNVELFGLAGSADIPVRFSRGDETFVRHFSLAGVSLDDMSGRPPVIHLGLVPWLRD
                     PVVAGTVKDSPAEKSGLASGDRILAVNGAGVSSVQEAVEAIGATPTGSPVRLTLSNIR
                     TPETERVVEIMPVTSEGRTVVGIRIRALPEMVVVRLSPVDAVAAGWRKVKSITTLQAR
                     GVSQMATGEASTKNLTGPIGIADMAGDAVRGGFVPFLEYLALISIAIGFMNLIPVPVL
                     DGGQLVVLGLEGLRGRDFSERTKNWIGKAGFGLMLVVFAFAMNNDLTRLFEGV"
BASE COUNT          442 a          699 c          679 g          381 t
ORIGIN      
        1 gtcgaaggcc ttgatgccgt gcgtctcaat ggtcgccaca gcgggacgct cgacgatgtg
       61 cacgacgagc acgtcgccgt cctgagagag cgacacgtcc ttgaagaggc cggagcggta
      121 gagcgagtgg atggcgcggg tgcccatgtc gggcgtgtac tcgtcgcctg tgcggaacgg
      181 aagatgcgaa aagaccgtac cgggttcggt acgcatcagg ccttcaactc gaatgtcgga
      241 aatgacaaaa gccgaggccc cctcggcgca caccatggcc gcaatgacag cggcggccgt
      301 cttcgtcaga ttggcagaca aaatgaattc tctctatatc gtcacgggtc acgccctccg
      361 cccttgcacg gtctgcttca ggttcatccc cgaagacgac cgcaaacggc ggcggcaaat
      421 ttgtgtaagg ttaccaaatc ttgaggctct ccgttagcgg gcggcgggac ctctttcacg
      481 acagaacggg taatccctgt cttcgaggcc gcattcacgc gccgcccgac tcccgccgag
      541 ccccgtcaca cgccctcgaa caggcgcgtg aggtcgttgt tcattgcaaa ggcaaagacg
      601 acgagcatca ggccgaagcc cgccttgccg atccagttct tcgtcctttc ggaaaagtcc
      661 cggccgcgca ggccctcgag gccgagcacc acgagctgtc cgccgtcgag caccggcacg
      721 gggatcaggt tcatgaagcc gatcgcgatc gagatgagcg caagatattc gaggaagggc
      781 acgaagccac cgcgaacggc gtcgcccgcc atgtcggcaa tgccgatcgg gcccgtcagg
      841 ttcttcgtgc tggcctcgcc cgtggccatc tggctaacgc ctctggcctg aagggtggtg
      901 atgcttttca ccttgcgcca acccgcggcg actgcgtcaa caggcgagag cctcacgacg
      961 accatctcgg gaagcgccct gatgcggatg ccgacaaccg tgcggccctc ggacgtcacg
     1021 ggcataatct ccaccacgcg ctcggtctcg ggcgtcctga tgttcgaaag cgtcagcctc
     1081 acgggcgagc ccgttggcgt cgcgcctatg gcctcaaccg cctcctggac cgagctcacc
     1141 cctgcgccgt tgacggccag aatcctgtcg ccgctcgcaa gccccgactt ttcggcgggc
     1201 gaatccttca ccgtccccgc cacgacgggg tccctgagcc agggcacgag gccgagatgg
     1261 atgacgggcg ggcgcccgct catgtcgtca aggctcacgc ctgcgagcga gaaatgcctg
     1321 acgaaggtct catccccgcg ggagaacctg acgggaatgt cggcgctgcc cgccagaccg
     1381 aaaagctcga cgttcatgtc cgtgatgccg agaaccggcg caccgttgac ggacagcacg
     1441 cggtcgccct gcctcacgtt ctcgctcgcc gcctgcgtgc ctgcgggcgg cgtgcccatg
     1501 acggccgcga agtcgggcgt tccgatcgcg ccgaccatca cgtagaggaa gaaggcgagg
     1561 acgaagttcg tagcgggacc cgcaaagagc acgaggccgc gcttccagcg ggcctgacgg
     1621 gtgtagagcc ttctgcggtc gtcttcagtg agatccgcat ccgcctcgtc ggccatcctc
     1681 acgtagccgc cgagcggcag ggccgagaag cagtattcag tctccccctt tctccttcgc
     1741 cagagcacgg ggcccatgcc gaaggagaat cgcgtcacct tgatgttgaa gaacttggcc
     1801 gtgaagaaat ggccgccctc gtgaacgatg acgatgatgc cgatggtgag tagaaagcct
     1861 gcgaggccca gaagaaagct catgatcaga tgcgggagat tggagtcgga cccaacaaga
     1921 tacacggttt gagccgcgca cgcgcggctc ctccgcgccc tgcggacgat tggttacgct
     1981 tttgcccggg aggatccgcc tcaggccgtc tgcagctcga cgagacgcgc gaaggcaccc
     2041 tgtgcgtaag ctccctatgg ggtaaggaat agttaggaaa taacttttca atcttcgtcg
     2101 tgcttgaaac cacgaatgat gtaattccaa gtgccaaagg aagctctaaa aaacccccat
     2161 ctctgaacga tgggggaagc agatttgttt aaacgagtga c
//
LOCUS       DBMDTI010000326         2201 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10903_length_2201_cov_1.786190, whole genome shotgun sequence.
ACCESSION   DBMDTI010000326 DBMDTI010000000
VERSION     DBMDTI010000326.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10903_length_2201_cov_1.786190"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="ACZPB6_10205"
     CDS             complement(<1..331)
                     /locus_tag="ACZPB6_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR1184871.1"
                     /translation="MTAHTEQYVDRMVEDRRHFHMNPEEGWLEFETTAYLINRLESLG
                     FEVLTGLQVINPDAVMGRKAEEVEAAIERARAAGVSEALLERMGGYTGCVGVLDTGRP
                     GPVTALRF"
     gene            complement(371..1657)
                     /gene="dcuC"
                     /locus_tag="ACZPB6_10210"
     CDS             complement(371..1657)
                     /gene="dcuC"
                     /locus_tag="ACZPB6_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HIR1184872.1"
                     /translation="MTTFTLWMAVIVTLLTIAALLKRWETRLVLFASGLLMATLSLDP
                     MAALNAFANNMTRGSLIMSICSSMGFAAAISITRCDVQLVSSCVKPLGRLGVFLLPAC
                     MLVASCVAVAVPSAAGSAAALGPTMIPLMVRAGFHPAIAGAAIIGSISPSTLSPGSSH
                     NVFIAQLADMKVMDLIVSFTPEILTVCALNIILVTAMVFVFKEYKKPGPDGVSAFAVE
                     ANAHWQLTDRPNYLMAVAPILPVVILFLGATCLPAIRMSIPAAMLIGTVYAMIVARAN
                     PATVTKEFFTGMGKGYANIMGIIIAAGTFAGGLQAAGVIDALISTLKESNDIARYAAN
                     FGPFVMGVLTGSGDAAGHAFNQAVTPHAASFGMDIASLGMVAALSGALGRMCSPLAGA
                     TIVVAGMTRMTPLDISKRMAIPMLINLVLLGFIFDL"
     gene            complement(1716..>2201)
                     /locus_tag="ACZPB6_10215"
     CDS             complement(1716..>2201)
                     /locus_tag="ACZPB6_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 family protein"
                     /protein_id="HIR1184873.1"
                     /translation="EVHPDMDAILCIDTTKGNRILNKRGFAITPTVRDGWILHVSEDL
                     LEIMETTTGEPAAVLPLTMQDITPYGYDVYHLNSILQPSVVTDRPCVGVAITTVTKVA
                     GCATGATHPVDVEETVRFVIETAKAFAAGKCEFCDEAEYALLQKKYGSMRHLRTMGRD
                     E"
BASE COUNT          407 a          595 c          820 g          379 t
ORIGIN      
        1 cgaagcgaag cgcggtgacg ggaccgggac ggcccgtgtc gagcacgccg acgcagcccg
       61 tgtagccgcc catgcgctcg aggagggctt cggagacgcc ggcagcgcgg gcgcgctcga
      121 tggcggcctc gacctcttcg gccttgcggc ccatgacggc gtcgggattg atcacctgaa
      181 ggccggtgag gacctcgaag ccgagagact cgaggcgatt gatgaggtag gccgtcgttt
      241 cgaattcgag ccagccttct tccggattca tgtggaagtg gcggcggtcc tcgaccatgc
      301 ggtcaacgta ttgttcggta tgtgcggtca tgatgatgtc cttgtgacgg ggtgtccggg
      361 ttgaaccgga tcagaggtcg aagatgaagc cgagaagcac gaggttaatg agcatcggga
      421 tggccatgcg cttcgagatg tcgagcgggg tcatgcgggt catgcctgcg acgacgatgg
      481 tggcgccggc gagcggcgag cacatgcggc cgagcgcgcc cgagagggcg gcgaccatgc
      541 cgaggctggc gatgtccatg ccgaagctcg cggcgtgcgg cgtcacggcc tggttgaagg
      601 cgtggcctgc ggcgtcgccc gagcccgtga ggacgcccat gacgaacggg ccgaagttgg
      661 cggcgtagcg ggcgatgtcg ttggattcct tgagggtgga gatgagcgcg tcgatgacgc
      721 ccgcggcctg aagaccgccc gcgaacgtgc ctgcagcgat gatgatgccc atgatgttcg
      781 cgtagccctt gcccatgccc gtgaagaatt ccttcgtgac ggtggcgggg ttggcgcggg
      841 cgacgatcat ggcgtagacg gtgccgatca gcatggcggc cggaatgctc atgcggatgg
      901 cgggcaggca ggtggcgccg aggaagagga tcacgacggg aaggatcgga gcgacggcca
      961 tgaggtagtt cgggcggtcg gtgagctgcc agtgcgcatt ggcttcaacg gcaaaggccg
     1021 agacgccgtc gggaccgggc ttcttgtatt ccttgaagac gaacaccatt gcggttacga
     1081 ggatgatgtt gagcgcgcag accgtcagga tctcgggcgt gaaggagacg atgaggtcca
     1141 tcaccttcat gtcggcgagt tgggcgatga agacgttgtg gctgctgccg ggagagagcg
     1201 tcgagggaga aatcgagccg atgatggcgg cgcccgcaat ggccggatgg aagcccgcgc
     1261 gaaccatgag cggaatcatc gtgggaccga gtgcggcggc ggaacctgcg gcgctcggaa
     1321 cggcgacggc cacgcagctc gccacgagca tgcaggcggg gagaaggaag acgccgagac
     1381 ggccgagcgg cttgacgcag gagctaacga gctgcacgtc gcagcgcgtg atcgaaatcg
     1441 cggcggcaaa gcccatggac gaacagatcg acatgatgag cgatccgcgg gtcatgttgt
     1501 tcgcgaaggc gttgagcgcg gccatcggat caagcgagag cgtggccatc agaaggccgg
     1561 aagcaaagag tacgaggcgc gtttcccatc gcttcagaag ggcggcgatc gtcagcagcg
     1621 tgacgatgac ggccatccag agtgtgaagg tggtcatggg tgggtccctt ggttgagttg
     1681 gatttggatt gtgtcggcag gccggagccc gatccttatt cgtcgcggcc catcgtgcga
     1741 agatggcgca tggagccgta cttcttctgc agaagcgcat actcggcctc gtcgcagaat
     1801 tcgcacttgc ccgccgcgaa ggccttggcc gtttcgatca cgaagcggac ggtctcctcg
     1861 acgtcgacgg gatgggtcgc acccgtggcg cagcccgcga ccttggtcac ggtcgtgatg
     1921 gcgacgccga cgcacgggcg gtccgtgacg acgctcggct gaagaatcga gttgaggtga
     1981 tagacgtcgt agccgtaggg cgtgatgtcc tgcatcgtga gcggaagaac ggctgcaggc
     2041 tcgcccgtcg tcgtctccat gatttcaagc agatcttcgc tcacgtgaag gatccagccg
     2101 tcgcgcacgg tcggcgtgat cgcaaagccg cgcttgttga gaatgcggtt gcccttcgtc
     2161 gtgtcgatgc agaggatggc gtccatgtcg ggatggactt c
//
LOCUS       DBMDTI010000327         2199 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10914_length_2199_cov_4.140610, whole genome shotgun sequence.
ACCESSION   DBMDTI010000327 DBMDTI010000000
VERSION     DBMDTI010000327.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10914_length_2199_cov_4.140610"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="ACZPB6_10220"
     CDS             <1..686
                     /locus_tag="ACZPB6_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1184874.1"
                     /translation="GMGKTTLLKLIGGLLTPQGGRVEVTGETLDPSNQKRLYRIRRRM
                     GMLFQFGALFTDLSVFDNVAFPMREQTDLDEETIRDLVLMKLNAVGLRGAAHLMPSEI
                     SGGMARRVALARAIALDPALIMYDEPFAGLDPISLGVTANLIRKLNDALHSTSVIVTH
                     DVVETFEIADYVYMINAGRVAAEGSPEELCRSEEPFVKQFINALPDGPVRFHYPANSI
                     EACFGGEER"
     gene            683..1459
                     /gene="mlaE"
                     /locus_tag="ACZPB6_10225"
     CDS             683..1459
                     /gene="mlaE"
                     /locus_tag="ACZPB6_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HIR1184875.1"
                     /translation="MRKLLTAIGRFVVGFFCRVGRFGIFSWTLLMRLGGARLSHVVQQ
                     IHFIGNYSLLIIAVSGLFVGFVLGLQGYYVLVTYGSEQALGTMVALSLVRELGPAVTA
                     LLFAGRAGTALTAEIGLMRAGEQLAAMEMMGVDPVRRVLAPRFIGAFVAMPVLATIFS
                     AVGIMGAWFVGVEMIGTDGGAFWSQMQSAVDVWHDIGNGFIKSVVFGVAVALVALFNG
                     YAARPTPEGVSRATTSTVVVASLLVLGLDFIMTAWMFTTV"
     gene            1480..1953
                     /gene="mlaD"
                     /locus_tag="ACZPB6_10230"
     CDS             1480..1953
                     /gene="mlaD"
                     /locus_tag="ACZPB6_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HIR1184876.1"
                     /translation="MDKNRTLEFYVGIFVFLGFAAMLFMALQAANLGSFTGSGKTISV
                     TAAFDNIGGLKARAAVKSAGVVVGRVQSVVFDPESFQAKVTMDLDAAYPFPADSSAKI
                     LTSGLLGEQYVGLEAGADEENLKNGDEIRRTQSAVVLETLISQFLYNTAEKTGKE"
     gene            1971..>2199
                     /locus_tag="ACZPB6_10235"
     CDS             1971..>2199
                     /locus_tag="ACZPB6_10235"
                     /inference="COORDINATES: protein motif:HMM:NF016236.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR1184877.1"
                     /translation="MKNTKILILTLSALSLGLSGCANVPPDAGQNPEDPYEAFNRNMH
                     AFNVMVDDAALKPVTKGYRWVTPQFVRDGVSN"
BASE COUNT          380 a          711 c          679 g          429 t
ORIGIN      
        1 cgggcatggg caagacgacg ctgctcaagc tgatcggcgg cctgctgacg cctcagggcg
       61 gcagggtcga ggtgacgggc gaaacgctcg atccctcgaa tcagaagcgc ctctaccgca
      121 tccgccgccg catgggcatg ctcttccagt tcggtgcgct cttcaccgac ctttcggtct
      181 tcgacaacgt tgccttcccg atgcgcgagc agacggatct cgacgaggag accattcggg
      241 atctggtgct catgaagctc aatgccgtgg gcctgagggg cgccgcgcac ctgatgccct
      301 ccgagatctc gggcggcatg gcgcgccgcg ttgcgctcgc acgtgcgatt gcgctcgacc
      361 ccgcgctcat catgtacgac gagccgttcg cgggccttga tccgatttcg ctcggcgtca
      421 ccgccaacct gattcgcaag ctcaacgatg cgctgcactc cacgtctgtc atcgtgaccc
      481 acgacgtcgt cgaaacgttc gagatcgccg actacgtcta catgatcaat gcgggccgcg
      541 tggcggccga gggctctccc gaggagctgt gccgttccga ggagccgttc gtcaagcagt
      601 tcatcaatgc gcttcccgac ggtcccgtcc gcttccacta cccggccaac agcatcgagg
      661 cctgcttcgg aggagaggaa agatgagaaa gctcctcacg gcaatcggcc gcttcgtggt
      721 cggattcttc tgcagggtcg gccgattcgg gatcttctct tggacgcttc tgatgcggct
      781 gggcggcgca aggctcagcc acgtcgttca gcagatccac ttcatcggca actactcgct
      841 tctcatcatt gcggtttcgg gcctcttcgt gggcttcgtg ctcggccttc agggctacta
      901 cgtgctcgtc acctacggca gcgagcaggc gctcggcacc atggtcgcgc tctcccttgt
      961 gcgagagctc ggccccgccg tgacggcgct gctcttcgcg ggccgggcgg gcactgcgct
     1021 caccgctgaa atcggcctca tgcgcgcggg cgagcagctc gcggccatgg agatgatggg
     1081 cgtcgacccg gtccgccgcg tcctcgcccc ccgattcatc ggcgccttcg tggcgatgcc
     1141 cgtgctggca acgatcttct cggccgtggg catcatgggc gcctggtttg tgggcgtgga
     1201 gatgatcggc accgacggcg gcgcgttctg gagccagatg cagagcgcag tcgacgtctg
     1261 gcacgacatc ggcaacggct tcatcaagag cgtggtcttc ggcgttgccg tcgcgctcgt
     1321 cgcacttttc aacgggtatg ccgcccgtcc gacaccggaa ggcgtctcca gagccacgac
     1381 gagcaccgtg gtggtggctt cgcttctcgt gctcggcctt gacttcatca tgacggcgtg
     1441 gatgtttacg accgtgtaag cgaatgggga atttttaaaa tggacaagaa tcgcacactc
     1501 gagttttacg tcgggatctt cgtgttcctc ggctttgctg ccatgctgtt catggcactg
     1561 caggcggcca acctcggcag cttcacgggc agcggcaaga cgatcagcgt cacggccgcc
     1621 ttcgacaaca tcggcggcct caaggcccgc gccgcagtga agtctgcggg cgtcgtggtg
     1681 ggccgcgtgc agtccgtcgt tttcgatccc gagtccttcc aggcgaaggt cacgatggat
     1741 ctcgacgccg cctatccctt cccggccgac tcctcggcca agatcctgac ttcgggtctt
     1801 ctcggcgaac agtacgtcgg ccttgaggcc ggcgccgacg aggagaacct gaagaacggc
     1861 gatgaaatcc gccgcaccca gagtgcggtc gtactcgaaa ccctgatcag ccagttcctc
     1921 tacaacacgg cggaaaagac cggaaaggaa tgaagcacgg ggtcacaaaa atgaaaaaca
     1981 cgaaaatcct cattcttact ctcagtgcct tatcgttggg actgtcgggc tgcgcgaacg
     2041 tgccccccga tgccggacag aatcccgagg acccgtacga ggcattcaac cgcaacatgc
     2101 acgcgttcaa cgtgatggtc gacgatgcgg cgctcaagcc cgtcacgaag ggctatcgct
     2161 gggtgacgcc gcagttcgtc cgggacggcg tctccaacg
//
LOCUS       DBMDTI010000328         2188 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_10990_length_2188_cov_1.701006, whole genome shotgun sequence.
ACCESSION   DBMDTI010000328 DBMDTI010000000
VERSION     DBMDTI010000328.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10990_length_2188_cov_1.701006"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="ACZPB6_10240"
     CDS             <1..633
                     /locus_tag="ACZPB6_10240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1184878.1"
                     /translation="DAESARELDRLAALHPEMTLLRHEVNRGKGGAFLTGLEHAASEG
                     YTHALQVDSDGQHDLADAPALLESARRHPGVLWSARPEYDESVPKKRLIGRYVTHVWV
                     WIETLSFEIVDSMCGYRVYPVADTLAVIKSRPVGLFMDFDTEIMVRLYWRGLPVRFIR
                     SRVIYPEGGRSNFRMWEDNVRISKMHARLFIESPLWWPARIRRLFRREPV"
     gene            643..1626
                     /locus_tag="ACZPB6_10245"
     CDS             643..1626
                     /locus_tag="ACZPB6_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015251.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family acyltransferase"
                     /protein_id="HIR1184879.1"
                     /translation="MTEAESKEGREGGEHWSTTPERRGMWGLRLLWLLYRIGGRPLFA
                     LIGTPVAFIFRLTGTRAREASARFLERVAERREALGLVLRPVSGHAHFRAFATSMLDR
                     LAAWAGDLKLARDVDFADEESRRLLCEPTPDGRGRLVLVSHLGVAEATRALAQHDRAV
                     PVWALIFDRHAPRFKAMLEAVAPDASRHVIAVDRLGVETASMLQDAIERGEWVAIAAD
                     RTPNGALTRGDRSVTADFLGREAPFPAGPFILASLLKCGVVTLFAVREGNRILIKCEP
                     FADEVKLPRRGREAELSRLASLWAQRLEAEALEHPAQWFNFYDFWHEEDRR"
     gene            1623..>2188
                     /locus_tag="ACZPB6_10250"
     CDS             1623..>2188
                     /locus_tag="ACZPB6_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA family protein"
                     /protein_id="HIR1184880.1"
                     /translation="MIRFIKKFAVFFIAALVLTFIWFKTAGAAETLDAARLEALAKRL
                     NDFTSLEADFTQKRTLGAAGRTLTSTGTLSLTGDGAFSWVQKTPFVQTVSLKDGVFTI
                     RYEDEAPEVMTAETHPAVLSAARLLTSVMRLDAAALRDAFDFTLVGGEKPADAWTITL
                     RARDEALGRILGTVTLKGSEYVESIRMSP"
BASE COUNT          352 a          743 c          697 g          396 t
ORIGIN      
        1 gatgccgaga gtgcgcgcga gcttgacagg cttgccgccc tgcatcccga gatgacgctt
       61 ctgcgccacg aggtcaaccg aggcaagggc ggggcgtttc tgacggggct cgaacatgcg
      121 gcctccgagg gctatacgca tgcgcttcag gtcgactccg acggccagca cgacctcgcg
      181 gacgcccccg cgctcctcga gtccgcccgc aggcatcccg gcgtcctctg gtcggcgcgt
      241 cccgaatacg acgagtccgt tccgaagaag cgcctgatcg gccgctacgt cacgcacgtc
      301 tgggtctgga ttgaaacgct cagcttcgag atcgtcgaca gcatgtgcgg ctaccgcgtc
      361 tatcccgtcg ccgacacgct tgccgtgata aaaagccgtc ccgtgggcct tttcatggac
      421 ttcgataccg aaatcatggt ccgcctctac tggcggggcc tgcccgtgcg cttcatccgc
      481 agccgcgtga tctatcccga gggcggcagg tctaacttcc gtatgtggga ggacaacgtg
      541 cgcatctcga agatgcacgc gcgcctcttc atcgaaagcc cgctctggtg gcccgcccgc
      601 atccgcaggc ttttccgccg cgaacccgtc tgaggagaag agatgaccga agccgaatcg
      661 aaggaaggca gggaaggcgg ggagcattgg agcaccacgc ccgaacgtcg cggcatgtgg
      721 gggctcaggc tgctgtggct tctctacagg atcgggggca ggcctctctt tgcgctcatc
      781 ggaactcccg ttgctttcat cttcaggctc acggggacgc gtgcccgtga ggcgagcgcc
      841 cgttttctcg agcgcgtcgc cgagcgcagg gaggcgctcg gtcttgtgct tcgccccgtc
      901 tcggggcacg cccacttcag agccttcgcc acgagcatgc tcgaccgtct tgccgcatgg
      961 gcgggcgacc tgaagctcgc gcgcgacgtc gacttcgccg acgaggaaag ccgcaggctt
     1021 ctctgcgagc ccacgcccga cggcaggggc aggctcgtgc tcgtctcgca tctgggcgta
     1081 gccgaggcga ctcgtgcgct cgcccagcac gacagggccg tgcccgtctg ggcgctgata
     1141 ttcgaccgtc atgcgccgag gttcaaggcg atgctcgagg ccgttgcccc cgatgcctcg
     1201 cgccacgtga tcgccgtcga ccgtctcggc gtcgagaccg cctcgatgct ccaggatgcc
     1261 atcgagcgcg gcgaatgggt cgcaatcgcg gccgaccgca cgcccaacgg tgcgctcacg
     1321 cgcggcgacc ggtccgtcac ggcggacttt ctcggccgtg aggcgccgtt tcccgccggt
     1381 cccttcatcc tcgcgtcgct cctgaagtgc ggcgtggtca cgctctttgc cgtgagggaa
     1441 gggaaccgca ttctcatcaa gtgcgagcct tttgcggatg aggtgaagct cccgaggcgc
     1501 ggccgcgagg ccgaactttc gcgcctggcc tctctctggg cgcagaggct tgaggccgag
     1561 gcgctcgagc atcccgccca atggttcaac ttctacgact tctggcatga ggaggatcgc
     1621 aggtgatccg ctttatcaag aaattcgccg tcttcttcat cgcggcactg gtcctcacct
     1681 tcatctggtt caagactgca ggcgccgccg agacgctgga cgccgcaagg ctcgaggcgc
     1741 tcgcgaagcg tctcaacgac ttcacttcgc ttgaggccga cttcacgcag aagcgcactc
     1801 taggcgccgc gggccgcacg ctcacgagca cgggcacgct gtcgctcacg ggtgacggag
     1861 ccttctcctg ggtccagaag acgcctttcg tacagacggt ctcgctcaaa gacggcgtct
     1921 tcacgatccg ctacgaggac gaggcgcccg aagtgatgac ggccgaaacc catcccgctg
     1981 ttctttccgc cgcacgtctt ctcacgtccg tgatgaggct cgacgcggcc gcgctgcggg
     2041 acgccttcga cttcacgctc gtgggcggcg aaaagcccgc cgacgcttgg acgatcacgc
     2101 tcagggcccg cgacgaggct ctcggccgca ttctcggcac cgtgacgctg aagggctcgg
     2161 aatacgttga gtcgatcaga atgtctcc
//
LOCUS       DBMDTI010000329         2177 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_11067_length_2177_cov_3.266378, whole genome shotgun sequence.
ACCESSION   DBMDTI010000329 DBMDTI010000000
VERSION     DBMDTI010000329.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11067_length_2177_cov_3.266378"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="ACZPB6_10255"
     CDS             <1..315
                     /locus_tag="ACZPB6_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HIR1184881.1"
                     /translation="RVEVLFVNEAGVMAETVEAPDGAAASEVLGLAGASLPAGAGLAV
                     FGRRIEPSTKLHEGDRLEVAGALVCDPKKVRRARAESQGDVRVVTCGRHGGRRRLAKT
                     AQ"
     gene            551..1981
                     /locus_tag="ACZPB6_10260"
     CDS             551..1981
                     /locus_tag="ACZPB6_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1184882.1"
                     /translation="MNWLNDIIGVINNYLWSYVLIVMLIGIGLWFTVRTRFVQIRCIP
                     EMFRLMTEGAGAKPREGHISTFQAFCVSTASRVGVGNIAGIAIAVATGGPGAVFWMWV
                     IATIGAASGFVESTLAQIYKAPRKGGGFVGGPAYYIGNVIGSSFFASVFAVLLAVTYG
                     LIFNSVQANTISLSMQTSFGFDTQMTGLAIAGFTALVIFGGLTRIAKVVGVMVPVMAG
                     AYILVALAVTALNIAMVPEVLVTIVRCAFDFDAVLGAGFGMALMTGIKRGLFSNEAGM
                     GAVPNAAATADATHPAKQGLVQALGVYFDTLVVCTASAMLVLLTPGWADSGKTGIELI
                     QQTLTGQLGTWTNAFMSVTVLFFAFSSIIGNYFYGEINMDFISRHPAALWIYRAFVVM
                     MVYLGSVASLGLVWNLADLFMALMAIVNLVAIAILGKYAFIALDDYVSQKKRGILNPE
                     FDPSVMPNHRGILCWPRHKEEEIEQC"
BASE COUNT          359 a          750 c          660 g          408 t
ORIGIN      
        1 cgtgtggaag tgttgttcgt gaatgaggcg ggcgtgatgg ccgaaacggt cgaagcgccc
       61 gatggcgccg cggcttccga ggtcctcgga ctagcgggcg catcgctgcc tgcgggggcg
      121 gggctcgctg tcttcggccg ccgcatcgag ccttcgacga agcttcacga gggcgatcgg
      181 ctcgaggtcg cgggcgcgct cgtctgtgat cccaagaagg ttcgccgcgc acgcgccgaa
      241 agccagggcg acgtgcgcgt cgtcacctgc ggccgccacg gcgggcgcag gcgcctcgcc
      301 aagactgcgc agtagtctgc agccggcttg tgtgcggcac ggaaaatgca gaaagggggc
      361 cttggcggac aacctcaggg caaacccttt gttacaatgg taaattgctg caaacaattc
      421 cggacgcatc cccactcagt ccggacgcgg aacggccgct cccccgaccg tccgtgcagg
      481 ggtgcggatt ccgccgcccc tgaagctgcg cctcctgaaa caaaaagacc gagaaggaaa
      541 accacaaaac atgaactggc tcaatgacat catcggcgtg atcaacaatt atctttggtc
      601 ctacgtcctc atcgtgatgc tgatcggcat cggcctctgg ttcacggtcc gcacgcgctt
      661 cgtgcagatc cgctgcattc ccgaaatgtt ccgcctgatg accgagggcg cgggcgccaa
      721 gccgcgcgag ggccacatct cgaccttcca ggccttctgc gtctccaccg cctcccgcgt
      781 gggcgtgggc aacatcgccg gcatcgccat cgcggtcgcc acgggcggcc ccggcgccgt
      841 cttctggatg tgggtgatcg ccacgatcgg cgccgcgtcg ggcttcgtcg agtccacgct
      901 cgcccagatc tacaaggccc cgcgcaaggg cggcggcttc gtgggcggtc ccgcctacta
      961 catcggcaac gtgatcggct cctctttctt cgcgagcgtc ttcgcggtgc tcctcgccgt
     1021 cacctacggc ctcatcttca actccgtcca ggccaacacc atcagcctct cgatgcagac
     1081 ctcgttcggc ttcgacacgc agatgacggg ccttgcgatc gcgggcttca cggccctcgt
     1141 gatcttcggc ggcctcaccc gcatcgccaa ggtcgtgggc gtgatggttc ccgtcatggc
     1201 cggcgcctac atccttgtcg cgctcgccgt cacggcgctc aacatcgcga tggtgcccga
     1261 agtgctcgtc acgatcgtgc gctgcgcctt cgacttcgac gccgtcctcg gcgcgggctt
     1321 cggcatggcc ctcatgaccg gcatcaagcg cggcctcttc tcgaacgagg ccggcatggg
     1381 tgcggtcccc aatgccgccg ccacggccga cgccacccac cccgccaagc agggcctcgt
     1441 gcaggcgctc ggcgtctact tcgacacgct cgtcgtgtgc acggcctccg ccatgctcgt
     1501 gctgctcacc ccgggctggg ccgactccgg caagaccggc atcgagctca tccagcagac
     1561 gctcacgggc cagctcggca cctggacgaa cgccttcatg agcgtcacgg tccttttctt
     1621 cgccttcagc tcgatcatcg gcaactactt ctacggcgaa atcaacatgg acttcatcag
     1681 ccgtcatccg gccgcgctct ggatctaccg cgccttcgtc gtgatgatgg tctacctcgg
     1741 ctccgtcgcc tcgctcggcc tcgtctggaa cctcgccgac ctcttcatgg ccctcatggc
     1801 catcgtcaat ctggttgcca tcgcgatcct cggcaagtac gccttcatcg cgctcgacga
     1861 ctacgtcagc cagaagaagc gcggcatcct caacccggaa ttcgatccga gcgtcatgcc
     1921 gaaccaccgc ggcatcctct gctggccgcg ccacaaggaa gaggagatcg aacagtgctg
     1981 atgccggcct gattgatcct gttccgacac tctgaaaacg cccgtcacgc gccgagatgc
     2041 gctgacgggc tttttcgtgc ccgaagacgg cggagagggg atggtggggg acggtggtga
     2101 gcatcacttt ggtattacct ctcgagaggt attttcgtgc ggaagactgt ggaacagggt
     2161 atccggcggt cattggg
//
LOCUS       DBMDTI010000330         2141 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_11329_length_2141_cov_3.057843, whole genome shotgun sequence.
ACCESSION   DBMDTI010000330 DBMDTI010000000
VERSION     DBMDTI010000330.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11329_length_2141_cov_3.057843"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1919)
                     /locus_tag="ACZPB6_10265"
     CDS             complement(<1..1919)
                     /locus_tag="ACZPB6_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family phospholipid transporter"
                     /protein_id="HIR1184883.1"
                     /translation="MKKIFFRRRSISYRGRGNFATQLSFSQPIVKLAVWLYFIICILV
                     IGTRVFFMTQADHYRDEITALVSEAAGVDVHAGEFSAGFERFWPVITVRQLELARPGG
                     PVSLTLPEVKARLAWSSLWHLEPRFQLLRISSPDLRITRLDKTTLDIAGFVVNLPEGS
                     DEESNFAASGDSRVAAWLLAQKKLELVNGTITYDDRYSPGAAEVRVMNANVVFEQTLL
                     DWRVAVDGTAHISAEESPHKQGECFTAVARIDRHIFTDTSNPLTWKGQAYVRFSHADV
                     AKLLSRAGFPQFMSSGSGAAEVWAGFADGRITSATADVALLDIHTQLADNLEPLKLAW
                     LESRLYYNGSFGDRAEQTFGVRKLDFMTADRRRLKPTDASVTIRTDSAGQISDGAITV
                     SRLDIGSLIALVPELPLDDAVRSFVTAHAPEGYLKDVSVAFSGNPSDPGNWRPAATFK
                     DLSLKAADGIPGIRAISGSISPLKNGEGFKVVLDSPKSTLSFPGIFRHPDMKFDTLKA
                     TAEIVPHPTLTVRLPSFEASNPDAALKGSGSWQATGGPGTLELNGTISRARATAVPYY
                     IPLVVGDDVLDWLEAGILGGSGSNGTFIVKGPLEHFPWDGKHKNDGHFAIEADMTGGR
                     LDFLPSHVRKADGSW"
BASE COUNT          384 a          601 c          759 g          397 t
ORIGIN      
        1 tcccagctgc cgtcggcctt cctgacgtgc gaggggagga agtcgagcct gccgcccgtc
       61 atgtcggcct cgatcgcaaa gtgtccgtcg ttcttgtgct tgccgtccca gggaaagtgc
      121 tcaagcggtc ccttcacgat gaaggtgccg ttggagccgc tgccgccgag aatgccggcc
      181 tctagccagt cgagcacgtc gtcgcccacg acgagcggaa tgtagtaggg cacggccgtc
      241 gccctggccc tgctgatcgt gccgttgagc tcaagcgtgc cggggccgcc cgtcgcctgc
      301 cagctgccgc ttcccttgag cgcggcgtcc ggattcgagg cctcgaagga cggaagacgg
      361 acggtgagcg tcggatgggg cacgatctcg gcggttgcct tgagcgtgtc gaacttcatg
      421 tcggggtgac ggaagatgcc ggggaaggaa agggtcgact tcgggctgtc gaggaccacc
      481 ttgaagccct cgccgttctt gaggggggaa atcgaacccg aaatggccct gatgccggga
      541 atgccgtccg cagccttgag ggagaggtcc ttgaaggtgg cggcgggacg ccagttgccg
      601 gggtcggagg gattgcccga gaaggcgacg ctgacgtcct tcaggtagcc ctcgggggcg
      661 tgtgcggtca cgaaggagcg cacggcgtcg tcaagcggaa gctcgggaac gagggcgatg
      721 agcgagccga tgtcgaggcg cgagacggtg atggcaccgt cggagatctg gcccgcgctg
      781 tcggtcctga tcgtgaccga ggcgtcggtg ggcttgaggc ggcgacggtc ggccgtcatg
      841 aagtccagct tccttacgcc gaaggtctgt tcggcgcgat cgccgaacga gccgttgtag
      901 tagaggcgcg attcgagcca ggcaagcttc aggggctcga gattgtcggc gagctgagtg
      961 tggatgtcga gcagcgcgac gtcggccgtg gcgctcgtga tgcggccgtc cgcaaagccc
     1021 gcccagacct cggcggcgcc cgaacccgag gacatgaact gcgggaagcc cgcgcgcgag
     1081 agaagcttcg cgacgtcggc gtgcgagaag cgcacgtagg cctgtccctt ccaggtcaac
     1141 gggttcgacg tgtccgtgaa gatgtggcgg tcgatgcggg ccacggcggt gaagcattcg
     1201 ccctgcttgt gcggactctc ctcggcgctg atgtgagcgg tgccgtcgac ggcgactcgc
     1261 cagtcgagaa gcgtctgctc gaaaacgacg ttagcgttca tgacgcggac ttcggcggcg
     1321 ccggggctgt agcggtcgtc ataggtgatg gtgccgttga cgagctcgag cttcttctgc
     1381 gcgagtaacc aggcggcgac cctgctgtcg ccggaagcgg cgaagttgga ttcctcgtcg
     1441 cttccttcgg ggagattgac gacgaagccc gcgatgtcga gcgtcgtttt gtcgagacgg
     1501 gtgatgcgca ggtcgggact gctgatgcgc aacagctgga aacgtggctc gagatgccag
     1561 agcgaggacc aggcgagtct tgccttgact tcggggagcg tgagcgagac ggggccgccc
     1621 ggccttgcaa gctcgagctg cctgacggtg atgacgggcc agaagcgctc gaagcctgcg
     1681 gagaattcgc ccgcatggac gtcgacgccc gcggcttcgg agacgagtgc tgtgatttca
     1741 tccctgtagt ggtcggcctg cgtcatgaag aaaacgcggg tgccgatgac caggatgcag
     1801 atgatgaagt agagccagac ggcgagcttc acgatcggct gggagaagct gagctgcgtc
     1861 gcgaagttgc cgcgacctct gtagctgatc gaacggcggc ggaaaaaaat tttcttcatc
     1921 ggcggcggtg cagagtctga gaccttttga cagaacccgt gcgccgcaag cctctcattt
     1981 caatgggagg taaggcgcac aaagttttcg aacgtgaagt aacattgtcc tatttatact
     2041 accacccctt cctacattgt cgaacttccg cttcgtgtga acgatcagca gaatcgtttt
     2101 ctcgaaaagg cgtttgagtt cggacgcacg ctctacaatg c
//
LOCUS       DBMDTI010000331         2114 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_11522_length_2114_cov_2.269747, whole genome shotgun sequence.
ACCESSION   DBMDTI010000331 DBMDTI010000000
VERSION     DBMDTI010000331.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11522_length_2114_cov_2.269747"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="ACZPB6_10270"
     CDS             complement(<1..236)
                     /locus_tag="ACZPB6_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR1184884.1"
                     /translation="MTQVPLMDRIHITEDYDVPRVINGGWQLSAGHALERPLDLADAS
                     RAFNRLIDMGFDTFDCADIYTGVEDFFGGIIRERR"
     gene            complement(273..617)
                     /locus_tag="ACZPB6_10275"
     CDS             complement(273..617)
                     /locus_tag="ACZPB6_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HIR1184885.1"
                     /translation="MTSDDTFELYDLEVTVEGDEKDFVCRHHAGHAFRVEGEDLVFPE
                     GGRFSMYALAALIPLLPAKERPTSPNDWMTTDALIACPDPNCGAKFRIRRTAKRILSH
                     AECTVVPLGEKA"
     gene            complement(659..>2114)
                     /locus_tag="ACZPB6_10280"
     CDS             complement(659..>2114)
                     /locus_tag="ACZPB6_10280"
                     /inference="COORDINATES: protein motif:HMM:NF015515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR1184886.1"
                     /translation="RPPTTITRQAPDMTTLRFHGSAAVLLLPLALFVAVTARLFISDA
                     AFDLTGLAAAGVLTLFSGSLLAKAPSEYWKAAVRGASSELTGTVVLILIASGIFSAMM
                     KASGVADGLVFLGGMLHLTGHAFTVFVLLASSLMAAATGSSIGTLLAAMPIFFPAGVA
                     LGADPTMLAGAVLSGAIFGDNLAPVSDVTVISSVTQRFSNGTPADIAGVVRTRLPYAM
                     IALAVSLPVYAFMGESLPVSEAALSGDASALLMLVPVAVLIAVAVRTRDVLMAISAGV
                     VTGTATGLLIGRLSFATIFAAVDGRLSGFLISGISNVSSIILLCVTLFAFTGIVREAG
                     VPDMMAKALETGAAGRPSALRTELFLSAATLVVTTLFAAVTSASVALVGSLTDDIGRA
                     SNVHPYRRSHILSGLANSLPVLMPFSAFVLITMAAVSGLEGAAAVTPFTLMTAAFYPI
                     ALFLVFGFMVLTGTGRMMETPEPEAAEAKTATAL"
BASE COUNT          395 a          582 c          816 g          321 t
ORIGIN      
        1 cggcgctcgc ggatgatgcc gccgaagaag tcctcgacgc ccgtgtagat gtcggcgcag
       61 tcgaaggtgt cgaatcccat gtcgatgagg cggttgaagg cgcgggacgc gtcggcgaga
      121 tcgagcggac gctcgagcgc gtggcccgcg gaaagctgcc agccgccgtt gatgacgcgc
      181 ggcacgtcat agtcttcagt aatgtggatt cggtccatga ggggcacttg ggtcatgatc
      241 atgtactcct ggttgtacgg ttgccggacc gctcaggcct tttcgcccag cggcacgacg
      301 gtgcattcag cgtgcgagag gatgcgtttt gcggtccgtc ggattctgaa ctttgctccg
      361 cagttgggat cgggacaggc gatgagcgca tcggtcgtca tccagtcgtt cggggaggtg
      421 gggcgttcct tggcgggcag tagcgggatg agcgccgcga gcgcatacat cgagaagcgt
      481 ccgccctcgg gaaagacgag gtcctcgcct tccaccctga acgcgtgtcc cgcatggtgg
      541 cggcagacga agtccttttc gtcgccttca acggtgacct cgaggtcgta gagttcgaac
      601 gtgtcgtcag atgtcatcgt ctgtttcctg tttgtcaatc ctgaatgtct ggcgttattc
      661 agagggcggt tgcagtcttg gcctctgcag cttcgggttc gggcgtttcc atcatgcggc
      721 ccgtgcccgt gagcaccatg aagccgaaga cgaggaagag cgcgatcggg tagaaggcgg
      781 cggtcatgag cgtgaagggc gtgacggcag ccgcgccctc gagtcccgag acggcggcca
      841 tcgtgatgag cacgaaggcc gagaacggca tcaggacggg aaggctgttc gctaggcccg
      901 agaggatgtg cgagcggcgg tacgggtgca cgttcgaggc gcggccgatg tcgtcggtga
      961 gagagccgac gagtgcgacg gaggcggagg tgacggctgc aaagagcgtc gtcacgacga
     1021 gcgtggcggc cgagaggaag agctcggtgc gaagtgcgga gggacggccc gccgcccccg
     1081 tttcaagggc cttcgccatc atgtcgggca cgcccgcctc gcgcacgatg cccgtgaagg
     1141 cgaagagcgt gacgcacagc aggatgatgg acgagacgtt gctgatgcct gagatcagaa
     1201 agcccgagag cctgccgtcg acggccgcga agatcgtggc gaagctgagg cggccgatga
     1261 gaaggcccgt ggccgtgccc gtcacgacgc ccgccgagat cgccatcagc acgtcgcggg
     1321 tcctgaccgc aacggcgatg aggacggcga cggggacgag catcagaagg gcggacgcat
     1381 cgccagagag cgccgcctcg gagacgggga gggattcgcc catgaaggcg tagacgggca
     1441 gggagacggc gagcgcgatc atcgcgtagg gaagccgggt gcgcacgacg cctgcgatgt
     1501 cggcgggcgt gccgttgcta aagcgctggg tgaccgacga gatgacggtg acgtcggaga
     1561 cgggcgcgag gttgtcgccg aagatggcgc ccgagaggac ggcgcccgcg agcatggtgg
     1621 gatccgcccc gagtgcgacg cccgcaggga agaagatcgg catggcggcg agaagcgtgc
     1681 cgatggacga gcccgtggcg gctgccatca gagaggaggc gaggagcacg aagaccgtga
     1741 aggcgtggcc cgtgaggtgg agcatccctc cgaggaagac gagcccgtcg gcgacgcccg
     1801 aggccttcat catggcggag aagatgcccg aggcgatcag gatgagcacg accgtgcccg
     1861 tcagctcgga cgaggccccg cgcacggcgg ccttccagta ttcggacggg gctttcgcga
     1921 ggagcgagcc cgagaagagc gtgaggacgc ctgcggctgc gaggcccgtg aggtcgaagg
     1981 cggcatcgga gatgaagagc ctggccgtga cggccacgaa gagcgcgagc ggaagaagga
     2041 gaacggcggc ggagccgtgg aatcgaagcg ttgtcatgtc gggtgcctgt cgggtgatgg
     2101 ttgtcggagg acgg
//
LOCUS       DBMDTI010000332         2085 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_11721_length_2085_cov_3.462198, whole genome shotgun sequence.
ACCESSION   DBMDTI010000332 DBMDTI010000000
VERSION     DBMDTI010000332.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11721_length_2085_cov_3.462198"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(152..799)
                     /locus_tag="ACZPB6_10285"
     CDS             complement(152..799)
                     /locus_tag="ACZPB6_10285"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIR1184887.1"
                     /translation="MNSRIYPHPFFAREGRPFIIGTFAVTAVLWLLDWNFLAFLSLVL
                     FVFCVQFFRDPARELPTDPKAVLSPVDGRVCKVQKAQDPLTGEDAMMISIFMNVFNVH
                     SQKSPVAGRVEEIVYKPGLFLNADLDKASTHNERNAVRVTTDEGRSVTFVQVAGLVAR
                     RILCYAKVGERLEKGERYGFIRFGSRVDVYLPLDAEITVSIGDKVTGVMSTIARL"
     gene            complement(944..1678)
                     /locus_tag="ACZPB6_10290"
     CDS             complement(944..1678)
                     /locus_tag="ACZPB6_10290"
                     /inference="COORDINATES: protein motif:HMM:NF016300.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR1184888.1"
                     /translation="MKKTVLALSAAAVCVASAAPVLAEGQNPMTAPVQISMQGEASMT
                     VANDEAVILFSAESRRPDAVSASNDAAKRGNAAIEALKRFSDKIDVGTTGLSTNPVYT
                     RAKEGEVSEIGAWEARETLRVTVRDVSVVSDVLAAVVGHMNYEGITFRVSDKAERAQR
                     DELLKAAVADALRQAGIVAESIGRKSSDVSVASVRIGSHSAGGPRYYAAPMMKAERAS
                     ANVAPAVSAGTTEVSLTVTVEARLPR"
BASE COUNT          401 a          620 c          665 g          399 t
ORIGIN      
        1 tggttatcga ggggattggt ggacttctta cgagaattgc gtttggtgtt cgtgctcatt
       61 gggtgtgccc atgccaagca aaccaaaaga gcccgttcgc tgtcgaacgg gctcttttgg
      121 aagattgtca gtggttcacg ttcctggctg atcagagacg ggcgatcgtg gacatcacgc
      181 cagtgacctt gtcgccgatc gaaacggtga tctcggcgtc gagcggcaga tagacgtcaa
      241 cgcgagagcc gaaacggatg aagccatagc gctcgccctt ttcgagacgt tcgcccacct
      301 tggcatagca gaggatgcga cgggcaacaa ggccggccac ttggacgaag gtcacgcttc
      361 gcccctcgtc agtcgtgacg cgaacggcgt tgcgctcgtt atgagtcgaa gccttgtcaa
      421 gatcggcgtt gaggaagaga ccgggcttgt agacgatctc ctcgacgcga ccagcgacgg
      481 gggacttctg gctgtggacg ttgaagacgt tcatgaagat cgaaatcatc atcgcatcct
      541 cgccggtaag aggatcctga gctttctgga ccttgcagac gcggccgtcg accgggctca
      601 gcacggcctt cggatcggtg ggaagttcac gggccggatc gcggaagaac tgaacgcaaa
      661 agacaaagag gacgagcgag aggaaggcga ggaagttcca gtcgagaagc cagagcacgg
      721 ccgtgacggc gaacgtgccg atgatgaagg ggcgcccttc acgggcgaag aagggatgcg
      781 gatagattcg cgagttcaat tcgatctccg gagagagctt gtggaaagtg cgcccatttt
      841 aatgaaaaaa gccgccttgt gcgcggattt cggctgaacg acggcgccgg ccgccccttc
      901 gggcgtccgg cacggagatt caggggcttg agacgaaccc ggctcagcgg ggcaaccggg
      961 cctcgaccgt cacggtgagc gatacctcgg tcgtgcctgc gctcactgca ggcgcaacgt
     1021 ttgccgaggc gcgctcggcc ttcatcatgg gggccgcgta gtagcgaggg ccgcccgcgc
     1081 tgtgcgaccc gatgcgcacc gaggcgacgc tcacgtcgga ggacttgcgg ccgatcgact
     1141 cagcaacgat tcccgcctgc cggagcgcat cggctacggc ggccttgagg agctcgtctc
     1201 gctgggcgcg ctcggccttg tcggagacgc ggaaggtgat gccctcgtag ttcatgtggc
     1261 cgacgacggc ggcgagcacg tccgagacga cgctcacgtc acgcaccgta acgcgcaggg
     1321 tctcgcgggc ctcccaggct ccgatctcgg aaacctcgcc ttccttggca cgggtataga
     1381 cgggattggt cgagagcccc gtcgtcccga cgtcgatctt gtcgctgaag cgcttgagcg
     1441 cctcgatcgc cgcgttgccg cgcttggccg catcgttcga ggccgagacc gcatcgggcc
     1501 tgcggctttc ggccgaaaag agaatgacgg cctcgtcgtt ggcgacggtc atgctcgcct
     1561 cgccctgcat cgagatctgg acgggcgccg tcatcggatt ctgcccttcg gcgaggacgg
     1621 gagcggctga tgcgacgcag acggcggctg cgctgagggc aagaacggtc ttcttcattc
     1681 gaggttctcc ttgttgagtc acggcagcga atgccgtttc tttcattatg agttccagcg
     1741 ataccctttc gcaggcacgt ttgcaacgat ttattgagga gcatccccgg tttttccgcc
     1801 tgcggacctg aagggcgcgc ccgcgccctg cgtttttcga gcctgcgccg ctccgaaaac
     1861 gccgctttct ctgcccgccg tttgatccca tcggcgacac cttagccttt ttccctagtt
     1921 gcaaacccgt ccgacctgtt acgattctta cagtcaggca aagtcctcac gcgcgtcgtc
     1981 cgcccaaaaa agcagcagaa gcctcatgag caaactgcaa ccgggacacc gcttccgaag
     2041 gacctgtcgc ataaaagagt caatcaggcg gcatcaagac cgcct
//
LOCUS       DBMDTI010000333         2083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_11737_length_2083_cov_2.504036, whole genome shotgun sequence.
ACCESSION   DBMDTI010000333 DBMDTI010000000
VERSION     DBMDTI010000333.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11737_length_2083_cov_2.504036"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="ACZPB6_10295"
     CDS             <1..639
                     /locus_tag="ACZPB6_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013752.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1184889.1"
                     /translation="SLNLSDLEMESYLASDMVARSFCRVSSTRHFISDSTILRFRHFI
                     EQHGLAQKFLERTVNLAAEAGACSFDMVATDGTFIEAPSSTKNKAHARDPEMASGKKV
                     NTWHFGMKEHIAACSESGIIYGTVAAPANEHDITHLGDLLKGLEKKVFLDSGYIGCHK
                     RAEIQAIPFKDVSWYIAARPSAWKKELSISENFGGELGHKGGSSNISGEKFS"
     gene            complement(665..1888)
                     /locus_tag="ACZPB6_10300"
     CDS             complement(665..1888)
                     /locus_tag="ACZPB6_10300"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HIR1184890.1"
                     /translation="MRRVVVTGMGGVTSLGQNWETVKAGLLAHRNAVRRMHEWDDIEG
                     LNARLGAPVLDFRLPEHYTRKRIRSMGRVSLLSTRATELALEQAGLIDDPVIKSGRTG
                     IAYGSCMGSTRAVADFGEMIVNRNTRGITATTYVQMMPHTTAVNTGLFFGITGRVIPT
                     PSACTSGSQAIGYAWEAIRHGYQDVMVAGGAEELCPSEVAVFDTLFTASTRNDEPELT
                     PRPFDANRDGLVIGEGACTLVLEELEHARARGARIHAELVGFATNCDATHITQPNSKT
                     MRICMEEALRTAGLAPSDIGYLSAHGTATSRGDCAESNATASLFGNRTPVSSLKSFFG
                     HTLGACGSIEAWLAIEMMREGWFSPTINLTDIDPECGELDYITGKERRIDTEFVQSNN
                     FAFGGINTSLIFKRM"
     gene            complement(1888..>2083)
                     /locus_tag="ACZPB6_10305"
     CDS             complement(1888..>2083)
                     /locus_tag="ACZPB6_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR1184891.1"
                     /translation="CVAPGLIDTGMVDMNEIAREHAMAMIPMQRIGTPEEVAAVVGFL
                     MDERASYITRQVINVNGGMI"
BASE COUNT          371 a          577 c          703 g          432 t
ORIGIN      
        1 tctctcaatc tgtctgatct tgagatggag tcatatttgg cgagcgacat ggtggcccgc
       61 agcttctgtc gcgtgtccag cactcgtcat ttcatctcag attcgacgat actgcgcttc
      121 cggcatttca ttgaacaaca tgggttggcg cagaaattcc tcgagcgcac ggtaaacctg
      181 gcagcagagg ccggagcctg ttcttttgat atggtggcga ctgacggcac ctttatcgaa
      241 gcgccgtcgt caacgaagaa caaggctcac gctcgagatc ctgagatggc ttcgggcaag
      301 aaggtcaaca cctggcattt cgggatgaag gagcacattg ccgcctgctc tgaatccggg
      361 atcatctatg gcacggtcgc cgcaccggct aatgaacatg acattactca tctgggcgac
      421 cttctgaagg ggcttgagaa gaaggtcttc cttgattccg gctacatcgg ttgccacaag
      481 cgtgctgaaa ttcaggcgat tcctttcaag gacgtgtcat ggtacattgc tgccaggccg
      541 agcgcatgga agaaggaatt gtcaatcagt gagaactttg gaggcgaact tggtcataaa
      601 ggaggatcct ccaacatttc aggagaaaaa ttctcctgac gtgttattcg gttggaattt
      661 cggcttacat tcgcttgaag atgagcgacg tgttgatgcc gccgaaggcg aagttgttgc
      721 tctggacgaa ctcggtgtcg atcctgcgct ccttgcccgt gatgtagtcg agttcgccgc
      781 attcgggatc aatgtcggtg aggttgatgg tcgggctgaa ccagccttcg cgcatcatct
      841 cgatggcaag ccaggcctcg atcgaaccgc aggcgccgag cgtgtggccg aagaagctct
      901 tgagggatga gacgggcgtc ctgttgccga atagcgacgc cgtggcgttc gactcggcgc
      961 agtcgccgcg cgaggtggcg gtgccgtggg cggacagata gccgatgtcg gagggtgcga
     1021 ggcctgcggt cctgagcgcc tcctccatgc agatgcgcat ggttttgctg ttgggctgcg
     1081 tgatgtgggt ggcgtcacag ttcgtcgcaa agccgacgag ttcggcgtgg atcctggcgc
     1141 cgcgggcgcg ggcgtgttcg agctcctcga gcacgagcgt gcaggcgcct tcgccgatca
     1201 cgaggccgtc acggttggcg tcgaagggac gcggcgtgag ctcgggctcg tcgttgcggg
     1261 tgctggcggt aaagagcgtg tcgaagaccg cgacctcgga ggggcagagc tcttcggcgc
     1321 cgcccgccac catgacgtcc tgatagccgt ggcggatggc ctcccaggcg tagccgatcg
     1381 cctgggagcc cgacgtgcag gcgctcggtg tcgggatgac gcggccggtg atgccgaaga
     1441 agaggcccgt gttgacggcc gtggtgtgcg gcatcatctg cacgtaggtc gtggcggtga
     1501 tgccgcgcgt gttgcggttg acgatcatct cgccgaagtc ggcaacggcg cgggtactgc
     1561 ccatgcagga accgtaggcg atgccggtgc ggccgctctt gatgacagga tcgtcgatga
     1621 ggcctgcctg ctcgagggcg agctcggtcg cgcgagtcga aaggagcgaa acgcggccca
     1681 tcgagcggat gcgcttgcgc gtgtagtgtt cgggcagcct gaagtcgagg acgggcgcgc
     1741 caagcctggc gttgaggccc tcgatatcgt cccattcgtg catgcggcgc accgcgttcc
     1801 tgtgggcgag aagccccgcc ttgaccgttt cccagttctg gccgagcgac gtgacgccgc
     1861 ccatgccggt gactacgact ctgcgcatca gatcatgcct ccgttgacgt tgatgacttg
     1921 gcgcgtgatg taggacgcac gctcgtccat gaggaagccg acgacggccg ccacttcctc
     1981 cggggtgccg atgcgctgca tcggaatcat cgccatcgcg tgctcgcggg cgatttcgtt
     2041 catgtcgacc atgcccgtgt cgatgaggcc gggggcgacg cag
//
LOCUS       DBMDTI010000334         2083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_11739_length_2083_cov_2.068618, whole genome shotgun sequence.
ACCESSION   DBMDTI010000334 DBMDTI010000000
VERSION     DBMDTI010000334.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11739_length_2083_cov_2.068618"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            191..1867
                     /locus_tag="ACZPB6_10310"
     CDS             191..1867
                     /locus_tag="ACZPB6_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HIR1184892.1"
                     /translation="MDWLAHLLTDVSSVPHIILVYSLVIAVGALLGRIRLFGVSLGVT
                     FVLFAAIAAGHFGFTVNPAVLYFLRDFGLTVFIYFIGLQVGPSFFSSFKSGGMVLNLL
                     TVLQVLLGVAVTIALYILFKDTLSLPQMLGVHYGAVTNTPGLGATQETLELFKYTGEN
                     IAVAYACAYPLGVIGTIGSAILIRFIFRIKLAEEDKAWDAEERAHHQEPVFFHVEVSN
                     RALEGRTIEQVRNFISRGFIASRILSGSSISSPNGSTVLHMDDCLRIVSQPDVKEAIV
                     AFFGRERTDIDLAEEKSPLVVRTLMVTRESVNGMRIEDLALNRLDGVNITRVFRAGMT
                     LFPSAKLHLQVGDQVYCVGPETAVARMEAKLGNQVKKLDHPNIITIFIGLLLGVIVGS
                     VPFAVPGLPVPLKLGLAGGPLIVAILLGRFGPTMRLVTFTTPSANMMMRETGICLFLA
                     SVGLAAGDGFVAAMTDGNGPLYMALGVLITVIPLLIVGVIARRFLHINYHSIVGLMAG
                     ATTDPPALAYAGTLSERNSSAVAYSTVYPLAMFLRILMGQLILVILWPFL"
BASE COUNT          341 a          756 c          567 g          419 t
ORIGIN      
        1 gtcgagtact gcatcaaccg cggccgcgtc gagcgcacgc tcgtctcctt cgacgtcgtg
       61 atctagcagc gggagccgac ggcgcccccg ctcaagggaa aacccgattc tcgtatcgct
      121 tccgttggcg taaaatgcgc gcttttccca aggcatcagg acagaacctc accgatcggg
      181 ttttcttgca atggactggc tggctcatct gctcacggac gtctcctccg tccctcacat
      241 cattctcgtt tactcgcttg tgatcgccgt aggcgcgctg ctcgggcgca tccggctctt
      301 cggcgtctca cttggcgtca ccttcgtgct cttcgcggcc attgccgcgg ggcacttcgg
      361 cttcaccgtg aatcctgccg tgctctactt cctgcgcgac ttcgggctca ccgtcttcat
      421 ctacttcatc ggtcttcagg tgggcccctc gttcttttcg tccttcaaga gcggcggcat
      481 ggtactcaat ctcctcaccg tcctgcaggt gctccttggc gtcgccgtga cgatcgcgct
      541 ctacatcctc ttcaaggaca cgctgtcgtt gccgcagatg ctcggcgtcc actatggcgc
      601 cgtcacgaac acgcccggtc tcggcgccac tcaggaaacg ctcgagctct tcaagtacac
      661 gggtgagaac atcgccgttg cctacgcatg cgcctatccg ctcggcgtca tcggcacgat
      721 cggctccgcg atcctcatcc gctttatctt ccggatcaag cttgccgaag aggacaaggc
      781 gtgggacgcc gaagagcggg cccaccatca ggaacccgtc ttcttccacg tcgaggtcag
      841 caaccgggcg ctcgaaggcc gcacgatcga acaggtgagg aacttcatca gccgcggctt
      901 catcgcctcc cgaatcctct cgggcagcag catctcgagt cccaacggct cgaccgtcct
      961 gcacatggac gactgcctgc gcatcgtgag ccagcccgac gtcaaggagg cgatcgtcgc
     1021 cttcttcgga cgcgagcgca ccgacatcga cctcgccgag gagaagtctc cgctcgtcgt
     1081 gcgcacgctc atggtcacgc gcgaatccgt caacggcatg cgcatcgagg atctcgcact
     1141 caaccgtctg gacggcgtca acattacccg cgtcttccgc gcgggcatga cgctctttcc
     1201 gtccgcgaag ctccaccttc aagtgggcga ccaggtctac tgcgtgggtc ccgagacggc
     1261 cgtcgcgcgc atggaggcca agcttggcaa ccaggtgaag aagctcgacc atccgaacat
     1321 catcacgatc ttcatcgggc ttctgctcgg cgtcatcgtc ggcagcgtgc cgtttgccgt
     1381 gcccggcctg cccgtcccgc tcaagctcgg cctcgcgggc ggtccgctca tcgtcgccat
     1441 cctgctcggc cgcttcggtc cgaccatgcg gctcgtcacc ttcacgacgc cgtcagccaa
     1501 catgatgatg cgcgagaccg gcatctgtct cttcctcgcc tccgtgggcc ttgccgcagg
     1561 cgacggcttc gtcgccgcca tgaccgacgg caacgggccg ctctacatgg cgctcggcgt
     1621 cctcatcacc gtaatcccgc ttctcatcgt gggcgtgatc gcaaggcgct tcctgcacat
     1681 caactaccac agcatcgtgg gcctgatggc gggcgccacc accgatcctc ccgccctcgc
     1741 ctacgcgggc acgctctccg agcgcaactc ctcggccgtt gcctactcga ccgtctaccc
     1801 gctcgccatg ttcctgagga tcctgatggg gcagctcatc ctcgtcatcc tctggccgtt
     1861 cctctgaaag aggcgaaggt cacactgaaa gccagtcgca gacaaaccga aggccgccgc
     1921 cggaagcgct ccgacggcgg cctttcaggt gtacgatgaa tgcctgcggc acgtccgcac
     1981 ccgacgtctt catcccgaac cgacagcagc ccatgagaat cacgctccga cagctctccg
     2041 tcttccgaag tctttacgag acgcaccgcg tctcggcctc cgc
//
LOCUS       DBMDTI010000335         2072 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_11806_length_2072_cov_2.062912, whole genome shotgun sequence.
ACCESSION   DBMDTI010000335 DBMDTI010000000
VERSION     DBMDTI010000335.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11806_length_2072_cov_2.062912"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="ACZPB6_10315"
     CDS             complement(<1..760)
                     /locus_tag="ACZPB6_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HIR1184893.1"
                     /translation="MFKKLHYKVRGLPTPLAGLALGIASLGWCLENALPLAGWGQTTG
                     AVIGLVMIGFLVLRFTAHHDTLWADLKHPVVGSIVPTFAMCLMVVSKTAGHWMPEAGV
                     VLWCAAVLLHLAALGIFVVNRLQEPRLELMVPSWFVPPIGIVVADVTFPEVAVLLPFA
                     KILFWMGAAAYAVLLPLMIYRLFFLPEVPNGAKPTIAILAAPASLLLAGYLTVEKDPS
                     LLLVALLFGIAILMTVVIYFAFWRLLRLQFSPGYA"
     gene            1050..>2072
                     /locus_tag="ACZPB6_10320"
     CDS             1050..>2072
                     /locus_tag="ACZPB6_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184894.1"
                     /translation="MDMEDKETAPGTVLLAVDVARRQAELRRRSVIMTEDPVEAAEWL
                     RTYRHPMTELISIGPWASYSAFNQGAGVDLPRLLLSDRVRSACAVEVAGLDYAAQQAK
                     YLFAFEDAAEWIEANVETRVMSVVVPLSIFATDPDALRAEFEEEKRLRFTNVKTKEGA
                     AKPRRILARWNVVKNLAKEAREAIGNWSSDYAAEKVRQQVAWPVPPSMEVDFPGCVFA
                     RYRTSAEIEPTRQRTHNTILFTGMAVHREIGRNRPYCRRHFTPGLLLGGPRNWPDYEI
                     GLVDVMSIPRAAALLGESFIRHAAWRLSPEDVVWCGDPSVLHDVKLSSDMRILSFTSC
                     RTEGSPH"
BASE COUNT          417 a          608 c          695 g          352 t
ORIGIN      
        1 ccgcgtagcc cggcgagaac tgaagcctca ggaggcgcca gaaggcgaag tagatcacga
       61 ccgtcatgag gatcgcgatg ccgaagagga gcgcgacgag aagaagcgac ggatccttct
      121 cgaccgtgag gtagcctgca agcagcaggc tcgcgggcgc ggccagaatc gcaatcgtgg
      181 gcttggcgcc gttggggacc tcggggagga agaagaggcg gtagatcatc agcggcagga
      241 gcacggcata ggccgcggcg cccatccaga agaggatctt cgcgaaggga agcagcacgg
      301 ccacctcggg gaaggtcacg tcggccacca cgatgccgat gggcggcacg aaccaggagg
      361 gcaccatgag ttcgagccta ggttcctgca ggcggttcac gacgaagatg ccgagcgccg
      421 ccagatgaag gagcaccgcc gcgcaccaga gcacgacgcc cgcttcgggc atccagtggc
      481 ccgcggtctt cgagaccacc atcaggcaca tcgcgaacgt cgggacgatg ctgcccacga
      541 cgggatgctt caggtcggcc cagagcgtgt cgtggtgcgc cgtgaagcga agcaccagaa
      601 agccgatcat gacgaggccg atcacggcac ccgtcgtctg gccccagccc gcgagcggca
      661 gggcgttctc gaggcaccag cccagactcg caatgccgag cgcgagaccg gcgagcggcg
      721 tgggcaggcc gcgaaccttg tagtgaagct ttttgaacat gggaattctc aagaaaaggg
      781 cgggattacc cttggtttgc ggggctattt tggcagaaaa ttcgttcgga ggcgtcgttt
      841 ttttcagaac cgatcgtttg gaaaatgcga ccgacataac ttcaatcgtt cggctttacc
      901 gaaagtctgg acatcctgcg tcctcacgac tttttgcagg tcttccgcag ggggaggcct
      961 ttttcggctt cttcgaatag aatatacctt ctataaaaca ttcaatgtta aggatagggc
     1021 ctgcccgtgg cagcgcccgc aactgcgcga tggacatgga agacaaggag acggccccgg
     1081 gaacggtgct tctggcggtg gacgttgcaa ggcgtcaggc ggaattgagg cgaaggagcg
     1141 tcatcatgac ggaggatccc gtcgaggcgg ccgagtggct tcgcacctac aggcatccga
     1201 tgactgagct catctcgatc gggccctggg cgtcgtacag cgccttcaat caaggtgccg
     1261 gcgtcgacct gccgaggctt ctgctctcgg accgcgtgcg ctcggcctgt gcggtcgagg
     1321 tggcggggct cgactacgcg gcgcagcagg cgaagtacct ttttgcattt gaggacgccg
     1381 ccgagtggat tgaggccaat gttgagacgc gcgtgatgag cgtcgtcgtg ccgctttcga
     1441 tttttgcgac cgacccggac gccctgaggg ccgaatttga ggaggagaag cgcctgaggt
     1501 ttacgaacgt gaagacgaag gaaggcgccg cgaagccccg ccgcattctc gcccgctgga
     1561 acgtcgtgaa gaacctcgcg aaggaggccc gcgaggcgat cggcaactgg tcctcggact
     1621 atgcggccga gaaggttcgt cagcaggtgg cctggcccgt gccgccctcg atggaggttg
     1681 attttccagg atgcgtattc gcccgctacc gcacttcggc cgagatcgag cccacgcgcc
     1741 agcgcacgca caacacgatc ctcttcacgg gcatggcggt tcaccgcgag atcgggcgca
     1801 acaggccgta ctgcaggcgg cacttcacgc cgggactgct tctgggcggc cctcgaaact
     1861 ggcccgacta cgagatcgga ctggtcgacg tgatgagcat tccgagggcg gccgcacttc
     1921 taggagagag cttcatcagg cacgccgcct ggcgtctttc gcccgaggac gtcgtctggt
     1981 gcggagaccc ttcggtcctg cacgacgtga agctcagctc cgacatgcgg attctgagct
     2041 tcacgtcgtg caggaccgaa gggtctccgc ac
//
LOCUS       DBMDTI010000336         2061 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_11881_length_2061_cov_3.396939, whole genome shotgun sequence.
ACCESSION   DBMDTI010000336 DBMDTI010000000
VERSION     DBMDTI010000336.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11881_length_2061_cov_3.396939"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="ACZPB6_10325"
     CDS             complement(<1..893)
                     /locus_tag="ACZPB6_10325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1184895.1"
                     /translation="MTTFSELGLSDSIVSSLTELGFEEPTPIQNEAIPALLTGADVLG
                     RAATGTGKTAAFSLPLLERHVLGQEADAPLVLVLTPTRELCLQVSEAFHNYGRKAGVL
                     VTPIYGGQDYSRQIRALRRGTHVVVATPGRALDHIGRGTLDLTNIKAVVLDEADEMLD
                     LGFADELDAIFDALPGHVQTALFSATLPPKIARIAEARLVDPVRITIAKEKTAEGETP
                     RVPQIAYVVGHNYRTTALGRILDLEAPELAIIFTRTRMEVDDLTESLRARGFSVEALH
                     GGLDQPTRDRVMKRARSGQIDA"
     gene            complement(1185..1796)
                     /locus_tag="ACZPB6_10330"
     CDS             complement(1185..1796)
                     /locus_tag="ACZPB6_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIR1184896.1"
                     /translation="MNIIATTRNVQGTGASRRLRRADKLPGIVYGGNLPATPIELEHN
                     PIFYALRKEKFHASILTMELDGKEELVVLRAFQMHPYKPQVMHIDFQRINPNEKVTMR
                     VPLHFQGEEESPAVKVSKCIVSHAVSAIEVTCMPKELPEFITVDLSKLEAGQTFHASM
                     LQLPEGIEVHAKSGEDIVLATVALPVEEEVPSAEGEAAETPAA"
     gene            complement(1873..>2061)
                     /locus_tag="ACZPB6_10335"
     CDS             complement(1873..>2061)
                     /locus_tag="ACZPB6_10335"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931310.1"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-phosphate from D-ribose 5-phosphate and ATP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR1184897.1"
                     /translation="SGAAVERIMNSALDELVVTDTIPLSAAAEACPKIRQVSCAAIIG
                     ETLSRIAREDSVSNLFTE"
BASE COUNT          373 a          541 c          768 g          379 t
ORIGIN      
        1 gcgtcgatct ggccggagcg ggcgcgcttc atgacgcggt cgcgggtcgg ctggtcaaga
       61 ccgccgtgaa gggcctcgac gctgaagccg cgggcgcgaa gcgattccgt gaggtcgtcc
      121 acctccatgc gggtgcgcgt gaagatgatg gcgagctcgg gggcctcgag gtcgaggatg
      181 cggccgagcg cggtcgtgcg gtagttgtgg cccacgacgt aggcgatctg cgggacgcgg
      241 ggcgtttcgc cttcggcagt cttttccttg gcgatcgtaa tgcggaccgg gtcgacgagg
      301 cgggcttcgg cgatgcgggc gatcttcggg ggcagcgtgg cggagaagag agccgtctgc
      361 acgtggccgg gaagggcgtc gaagatggcg tcgagctcgt cggcaaagcc cagatcgagc
      421 atttcgtcgg cttcgtcaag cacgacggcc ttgatgttcg tgaggtcgag cgtgccgcgg
      481 ccgatgtggt cgagcgcgcg gcccggcgtg gcgacgacga cgtgcgtgcc acggcgcagg
      541 gcacggatct ggcggctgta gtcctgaccg ccgtagatcg gggtcacgag cacgccggcc
      601 ttgcggccgt agttgtggaa cgcctcgctc acctgcaggc agagttcacg ggtcggggtg
      661 agaacgagga cgagcggcgc atcggcttcc tgaccgagca cgtggcgctc aaggagcgga
      721 agcgagaagg cggcggtctt gcccgtgccc gtggcggcgc ggccgagcac gtcggcgccg
      781 gtcaggaggg cgggaatggc ctcgttctga atgggggtgg gttcctcgaa gccgagttcg
      841 gtcagggagg aaacgatgga gtcggacagg ccgagttcgg agaaagtcgt catgagttcg
      901 tgttgaaaat gttctgacgg ccgcccgcag gtatgcaggg aagcaagacg gacatggcgg
      961 acgtcgggag aagggaatgg atccgttttc cccgcacata acgaacgacg cgaactttct
     1021 gaaaggggca aacggcttgt ggcggcgctt caggcagcgg ccgcggaggt gtggaagacc
     1081 gtgagacggt cttcggaagg ggcgggccct tcgtacaaaa aagatccgca gggagtaggt
     1141 ccctgcggat cctgtcaaag caactgaatc agatcagaaa cttattaggc agccggggtt
     1201 tcggcggctt cgccttcagc ggaggggact tcttcctcga ccgggagggc aaccgtggcg
     1261 agcacgatgt cttcgccgga cttggcgtgg acttcgatgc cttccggaag ctgaagcatg
     1321 gaggcgtgga acgtctggcc ggcttcgagc ttggagaggt caacggtgat gaattccggc
     1381 agttccttcg gcatgcaggt cacttcgatg gcggacacgg catgggaaac gatgcacttg
     1441 gagaccttga cggccgggga ttcttcttca ccctggaagt gcaggggcac gcgcatcgtg
     1501 accttttcgt tcgggttgat gcgctggaag tcgatgtgca tgacctgcgg cttgtacggg
     1561 tgcatctgga aggcgcggag aacgacgagt tcttccttgc cgtcgagttc catcgtcagg
     1621 atcgaggcgt ggaacttttc cttgcgcaga gcgtagaaga tcggattgtg ttcgagctcg
     1681 atcggcgtag cggggaggtt gccgccgtag acgatgcccg gcagcttgtc cgcgcggcga
     1741 aggcggcggc tcgcacccgt accctgcacg ttacgcgtgg tggcgatgat gttcattgga
     1801 ttactcctaa aagttaagaa aaaaggttcc cgcggtcgcg accaactgcg ggaacgagga
     1861 aaaaactgtt ccttattccg tgaacaggtt gctcacggag tcttcgcggg cgatgcgcga
     1921 aagcgtttcg ccgatgatcg cggcgcagga gacctggcgg atcttcgggc aggcttcggc
     1981 ggcggcagac agcggaatcg tgtccgtaac gaccagctcg tcgagagcgg aattcatgat
     2041 gcgttcaacg gcggcgccgg a
//
LOCUS       DBMDTI010000337         2053 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_11936_length_2053_cov_2.415471, whole genome shotgun sequence.
ACCESSION   DBMDTI010000337 DBMDTI010000000
VERSION     DBMDTI010000337.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11936_length_2053_cov_2.415471"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="ACZPB6_10340"
     CDS             complement(<1..1162)
                     /locus_tag="ACZPB6_10340"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1184898.1"
                     /translation="MTNCLRPADPVGRLLTDPEVATPAFVIDDAKLQVNLDCAKEKAA
                     RLGVTLRPHLKTHKSIEIARRQMLSPEGPATVSTLAEARYFAENGVKDLIYAVGIAPQ
                     KLADVAAIREAGCDLKIILDNVAAAKAVSAFCAGRGVTIPVLIEVDCDGHRSGVRPES
                     ELLIEIARALKDGAELAGVLTHAGASYDVENLAVIRWAAANERDGIVTAANRLREAGF
                     EVRIVSIGSTPTMTHAEDETGVTEIRAGVYALGDLFMMNLGVVPMDRIAGTVLCTVIG
                     HQPEKGQVIVDAGWMAMSRDRGTARQHCDFGYGLVCDVDGRPLRGCDIRMTGANQEHG
                     IIEAAAGDRLKPEDLPVGTRLRIMPNHACPTAAPYEKLLLVNEEGRVTAALDH"
     gene            complement(1333..>2053)
                     /locus_tag="ACZPB6_10345"
     CDS             complement(1333..>2053)
                     /locus_tag="ACZPB6_10345"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR1184899.1"
                     /translation="ARPRGPGMERADLLEANAAIFRQQGQALNVAASRNVKVLVVGNP
                     CNTNAWIAMKNAPDLDPKCFSAMLRLDQNRAMSQLAEKTGAPVASIEHMAVWGNHSPA
                     MVPDITFATVEGKAAPSLVDDEWVKNDFMPTVAKRGSAIIAARGASSAASAGSAAIDH
                     IHDWFCGSEGRWVTMGVPSDGSYGVPEGMVFGFPCIVKEDGSYEIVKGLELTEDTKAR
                     IAKNVAELQGEQQAVAAFIPN"
BASE COUNT          306 a          616 c          766 g          365 t
ORIGIN      
        1 cgtgatcgag cgcggccgtc acgcgtccct cctcattgac gagaaggagc ttttcatagg
       61 gcgccgcggt cgggcaggcg tggttgggca tgatgcgaag gcgcgtgccg acgggcaggt
      121 cctcgggctt gagtcggtcg cctgcggcgg cctcgatgat gccgtgttcc tgattggcgc
      181 ccgtcatgcg gatgtcgcag ccgcgaagcg gcctgccgtc aacgtcgcag acgaggccgt
      241 agccgaagtc gcagtgctgg cgggcggtgc cgcggtcgcg ggacatggcc atccagcccg
      301 cgtcgacgat cacctggccc ttttcgggct ggtggccgat gacggtgcag agcaccgtgc
      361 ctgcaatgcg gtccatcggc acgacgccga gattcatcat gaagaggtcg ccgagcgcat
      421 agacgcccgc gcggatttcg gtcacgcccg tctcgtcctc ggcatgggtc atggtgggcg
      481 tcgagccgat cgagacgatg cgcacctcaa agcccgcctc cctcaggcgg ttcgcggccg
      541 tcacgatccc gtcgcgctca ttggcggcgg cccagcggat gacggcgagg ttttcgacgt
      601 cgtagcttgc gcccgcgtgc gtgagaacgc ccgcgagctc ggcgccgtcc ttaagggcgc
      661 gcgcgatctc gatgaggagt tcgctttcgg gcctgacgcc cgagcggtgg ccgtcgcagt
      721 cgacctcaat gaggacggga atcgtgacgc cgcgcccggc acagaaggcg ctgacggcct
      781 tcgcggccgc gacgttgtcg aggatgatct tcagatcgca cccagcttcg cggatggcag
      841 cgacgtcggc gagcttctgc ggggcgatgc ccacggcgta gatcaggtcc ttcacgccgt
      901 tttcggcgaa gtagcgggcc tcggcgagcg tggagacggt ggcgggtcct tcgggcgaaa
      961 gcatctggcg gcgggcgatc tcgatcgact tgtgggtctt gagatgcggg cgcagggtca
     1021 cgccgagacg ggcggccttc tcttttgcgc agtcaaggtt cacctgcagc ttcgcgtcgt
     1081 cgatgacgaa ggcgggggta gcgacttcgg ggtcggtaag cagacggccg acggggtcgg
     1141 cgggacggag acagttggtc atggattaac tctcctcaag gctgtgggct gtgtcctgcg
     1201 aaggtggtcg gcctgcgcgg ggatcctgca gatgtgatca agcccaccgt acgcctcgcg
     1261 ggcgtccgat gggctcggaa cggaactcgc cacgggcgcc tgcaggtgcg tgcgccggcg
     1321 gcggatcgtc gatcagttcg ggatgaaggc ggcgacggcc tgctgttcac cctgaagttc
     1381 ggcgacgttc ttcgcgatgc gggccttcgt gtcctcggtg agttcgagac ccttgacgat
     1441 ttcgtacgag ccgtcctcct tcacgatgca ggggaagccg aagaccatgc cttcggggac
     1501 gccgtaggag ccgtcggacg gaacgcccat cgtgacccag cggccttcgg agccgcagaa
     1561 ccagtcgtgg atgtggtcga tggcggcact gccggcggaa gcggcggagc tcgcgccgcg
     1621 ggcggcgatg atggcggagc cgcgcttggc gacggtcggc atgaagtcgt tcttcaccca
     1681 ttcgtcatcg acgagggacg gagcggcctt gccttcgacc gtggcgaacg tgatgtcggg
     1741 aaccatggcc ggggaatggt tgccccagac ggccatgtgc tcgatcgagg cgacgggggc
     1801 gcccgtcttt tcggcgagct gcgacatggc gcggttctgg tcgaggcgga gcatagcgga
     1861 gaagcacttc ggatcaagat ccggggcgtt cttcatggcg atccaggcat tggtgttgca
     1921 cggattgccg acgacgagca ccttgacgtt gcggctggcg gcgacgttga gggcctggcc
     1981 ctgctggcgg aagatggcgg cgttggcctc tagcaggtcg gcgcgttcca tgccgggacc
     2041 gcgcggacgg gcg
//
LOCUS       DBMDTI010000338         2053 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_11937_length_2053_cov_2.031762, whole genome shotgun sequence.
ACCESSION   DBMDTI010000338 DBMDTI010000000
VERSION     DBMDTI010000338.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11937_length_2053_cov_2.031762"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="ACZPB6_10350"
     CDS             <1..1156
                     /locus_tag="ACZPB6_10350"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1184900.1"
                     /translation="LFAVSTDLLRETSSDLFSGFVKVDMRVDDETGLVSSVTPMPGRV
                     FAAGLSVARMGLLEFAATLFALDIAPDIRWVRPADYRSVHLAAERMRDPVYMSYQKKT
                     LLEEMREHAAWVILAAAILIGMIWHSVAAERLVRRRSAELMETVKRQQASERQFEALE
                     RMTAVSQMSNIVAHELRQPLAAVTNFAMGVRRRIANGTLTADSLEFALGRILAENERA
                     SDIVEHVRDYARKRRRQITPVDLTQMSAKVVSSMRASNARVSFESEVPAGLFVEGDSL
                     EIELVLRNLVKNAGESAAQADGAPRVVLAGERIGDEVVITVTDNGPVRTKAEIDAFLV
                     PLRSEKSGGLGLGLAIVRRIIEAYGGTIVFDVVEPQGVRVEARLPARPDL"
     gene            1182..1856
                     /locus_tag="ACZPB6_10355"
     CDS             1182..1856
                     /locus_tag="ACZPB6_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1184901.1"
                     /translation="MEKINLSDLLEPEHMDPVKRIALIRIVDDDGGVRESYKFLIESE
                     GWRVKTYPSAEAFLEEDDPTVPGCGVFDVRMTGITGMELHQKLIELANRLPVIFVSAH
                     GDIEMAVKAMRRGAVDFLTKPVVDEKLLSSIDRAVARSCEEAAEAETVRELAGLWQAL
                     SPRECQVAQLAAEGLTTKAVSDVLGIAERTAQVHVAHVCSKLGVENAVGVAQIIARLR
                     ARGVLQ"
     gene            1853..>2053
                     /locus_tag="ACZPB6_10360"
     CDS             1853..>2053
                     /locus_tag="ACZPB6_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184902.1"
                     /translation="MKRLLRSDGFDVIVVGSGLAGLSAALTVAEFGYSPVILEKACEP
                     GGLTRRAYGFFNAADARRQIALG"
BASE COUNT          349 a          714 c          657 g          333 t
ORIGIN      
        1 gctcttcgcg gtctcgaccg acctgttgcg cgagacgagt tccgacctct tcagtggctt
       61 cgtcaaggtc gacatgcgcg tcgacgacga gacggggctc gtgagttccg tgaccccgat
      121 gccgggacgc gtgttcgcgg cgggtctttc ggtcgcgcgc atggggctgc tcgaatttgc
      181 cgcaacgctc ttcgcgcttg acatcgctcc cgacatccgc tgggtgcgtc ctgcggacta
      241 tcgctccgtg catctggcgg ccgagcgcat gcgcgatccc gtctacatga gctatcagaa
      301 gaagacgctt ctcgaggaga tgcgcgagca cgccgcctgg gtgatcctcg ccgcggcgat
      361 cctcatcggc atgatctggc attcggtcgc ggccgagcgg ctcgtgcgtc gccgcagcgc
      421 cgaactcatg gagacggtta agcgccagca ggcctccgag cgccagttcg aggccctcga
      481 gcgcatgacg gccgtctccc agatgtcgaa catcgtggcc catgagctca ggcagccgct
      541 cgccgccgtc accaacttcg cgatgggcgt caggcgccgc atcgccaacg gcacgctcac
      601 cgccgactcg ctcgaattcg cgctcggcag gatcctcgcc gagaacgagc gcgcgagcga
      661 catcgtcgag cacgtgcgcg actacgcccg aaagcgccgc cgccagatca cccccgtaga
      721 cctcacgcag atgagcgcca aggtcgtttc ctcgatgcgc gcctccaacg ccagggtctc
      781 cttcgagtcc gaggtgcccg caggcctctt cgtcgagggc gattcgctcg aaatcgaact
      841 cgtgctcaga aacctcgtga agaatgcggg cgagagcgcc gcacaggcgg acggggcgcc
      901 ccgcgtcgtc ttggcgggcg aaaggatagg cgatgaagtc gtcatcaccg tcaccgacaa
      961 cgggcccgtg aggacgaagg ccgagatcga cgccttcctc gttcctctca ggagcgagaa
     1021 gtcgggcggc ctcggactcg gccttgcgat cgttcgccgc atcatcgagg cctacggcgg
     1081 tacgatcgtc ttcgacgtcg tcgagccgca gggcgtcagg gtggaggcga ggcttcccgc
     1141 ccgccccgat ctctgagcct caacaccaag aaaatcgaat catggaaaag atcaatcttt
     1201 cggatctcct agaaccggag cacatggatc ccgtcaagcg catcgcgctc attcgcatcg
     1261 tcgacgacga cggcggcgtg cgcgagtcct acaagttcct gattgaaagc gagggctggc
     1321 gcgtgaagac ctacccgagc gccgaggcct tcctcgagga ggacgacccg acggtgccgg
     1381 gctgcggcgt cttcgacgtc cgcatgacgg gcatcacggg catggagctg caccagaagc
     1441 tcatcgagct cgcaaaccgc ctgcccgtca tcttcgtgag cgcccacggc gacatcgaga
     1501 tggccgtcaa ggcgatgagg cgcggcgcgg tggacttcct cacgaagccc gtcgtcgacg
     1561 agaagctcct ctcctcgatc gaccgcgccg tcgcaagaag ctgcgaggaa gcggccgagg
     1621 ccgaaacggt ccgcgaactt gcgggcctct ggcaggcgct cagtccccgc gagtgccagg
     1681 tcgcgcagct cgccgccgag ggcctcacca cgaaggccgt ctccgacgta ctcggcatcg
     1741 ccgagcgcac cgcacaggtc cacgtcgccc acgtctgctc gaagctcggc gttgagaacg
     1801 ccgtgggtgt cgcgcagatc atcgcgcgcc tgcgcgcccg cggagtcctg cagtgaagcg
     1861 gctcctcaga tccgacggct tcgacgtcat cgtcgtgggc tcgggacttg cgggcctctc
     1921 cgccgcgctc accgtcgccg agttcggcta cagtcccgtc atcctcgaga aggcctgcga
     1981 gcccggcggc ctcacccgcc gcgcctacgg cttcttcaac gccgcggacg cccgcaggca
     2041 gattgcgctc ggc
//
LOCUS       DBMDTI010000339         2049 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_11969_length_2049_cov_2.812115, whole genome shotgun sequence.
ACCESSION   DBMDTI010000339 DBMDTI010000000
VERSION     DBMDTI010000339.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11969_length_2049_cov_2.812115"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="ACZPB6_10365"
     CDS             <1..681
                     /locus_tag="ACZPB6_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HIR1184903.1"
                     /translation="RPVTIDSERFLQTVDAIAAALLDNDEIEGDMLEACRAIDWTRFA
                     SAELLESAGRDPMGYLHAVEVHAGEDALLDIFLLPVLGLALRVFLDEAADEGSRRIAR
                     TGTDTTHHNRPLHCPVCGSDAAVASVTDTPLNGHIKQLWCTCCGAHWVFERIRCAVCG
                     DQAVSDLSYIHDETDETRRLHVCAGCHAAFPTIFAGEELNFCADVEQIVMTGLELFYQ
                     NAVEGKEA"
     gene            737..1618
                     /gene="fdhD"
                     /locus_tag="ACZPB6_10370"
     CDS             737..1618
                     /gene="fdhD"
                     /locus_tag="ACZPB6_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR1184904.1"
                     /translation="MGCAYLNKAEHALATAIEAGTCMPVSMGGVERLGAREVEVFRMN
                     ADEPALDWVAEEVPVALVYNGISHAVMMASPSMLEEFALGFSLAEGIIPDASHLYACE
                     VREACRGGIEVDLTISSECFWKLKDRRRSMTGRTGCGICGVESLGEAVREKRVVPHEQ
                     TFPMAAYADALGYLERVERLGGITGCTHAAVWVRPDGSFAGGAEDVGRHVALDKLLGL
                     RARRGWRDGALVISSRASYEMVQKAAMCGVEIIFAVSAPTALAVELARECGITLAAFC
                     RRSRANVYSHPERLIGL"
BASE COUNT          358 a          653 c          641 g          397 t
ORIGIN      
        1 cgtcccgtca cgattgactc ggagcgcttc ctgcagacgg tcgacgccat cgcggcggcc
       61 cttctcgaca acgacgagat cgagggcgac atgctcgaag cctgccgagc catcgactgg
      121 acgcgctttg cgtcggctga gctccttgag agcgcaggtc gcgatccgat ggggtatctc
      181 catgccgtcg aggtgcacgc gggcgaggac gcccttcttg acatcttcct tcttcccgtg
      241 ctcggcctcg cgcttcgcgt cttccttgac gaggcggccg acgaaggcag ccgccgcatc
      301 gccagaacgg gcaccgacac gacgcatcac aaccgtccgc ttcactgccc cgtctgcggc
      361 tccgacgccg ccgtcgcgag cgtgaccgac acgccgctca acgggcacat caagcagctc
      421 tggtgcacgt gctgcggcgc acactgggtc ttcgagcgca tccgctgcgc cgtctgcggc
      481 gatcaggccg tgtcggacct ctcctacatc catgacgaga ccgacgaaac gcgccgcctg
      541 cacgtctgcg ccggctgtca tgccgccttc ccgacgatct tcgcgggaga ggagctcaac
      601 ttctgcgccg acgtcgagca gatcgtcatg acggggcttg agctcttcta tcagaacgcc
      661 gttgagggca aggaagcctg aggatcaata tttccgcaac gagacgccgc tggcacgaaa
      721 cgcacgggag atgaacatgg gatgcgctta tttgaacaag gccgaacacg cgcttgcgac
      781 cgccattgag gcggggacct gcatgcccgt ctccatgggc ggcgtcgagc gtctgggggc
      841 ccgcgaggtc gaggtctttc gcatgaatgc agacgagcct gcgctcgact gggtggcaga
      901 ggaggttccc gttgcgcttg tctacaacgg catctcgcac gccgtcatga tggcctctcc
      961 ctcgatgctc gaagaattcg cgctcggctt ctcgcttgcc gagggcatca ttcccgacgc
     1021 ctcgcatctc tatgcctgcg aggtgcgcga ggcctgccgc ggcggcatcg aggtggacct
     1081 cacgatttcg tccgaatgct tctggaagct caaggaccgc cgccgctcga tgacgggccg
     1141 cacgggttgc ggcatctgcg gcgtcgaaag tcttggagag gccgtgcgcg aaaagcgggt
     1201 cgtgccgcac gagcagacct tcccgatggc ggcctatgcc gacgccctcg gctatctgga
     1261 gcgtgtcgag cgcctcggcg gcatcacggg ctgcacccac gccgccgtct gggttcgtcc
     1321 cgacggaagc tttgcgggcg gcgccgaaga cgtcggcagg catgtcgcgc ttgacaagct
     1381 gctcggtctt cgtgcccgca ggggctggcg tgacggcgcg cttgtcatca gctccagagc
     1441 ctcctacgaa atggttcaga aggcggccat gtgcggcgtc gagatcatct tcgccgtctc
     1501 cgcaccgacg gcgctcgccg tcgaacttgc acgagagtgc gggatcacgc ttgcggcctt
     1561 ctgccgtcgc agccgcgcca acgtctactc ccatcccgag cgcctcattg ggctctgaga
     1621 gaaaaagggc gaagacggaa caagttgtct tggaatgcct tccggttttt cctttttctc
     1681 cggaaggcct tttgacaggc ggaggactgg tctcacggtc ttccgcctct ttttcgtctg
     1741 ttgtcgtcag aaggcggcgt taccctgcgg ggcagacgcc tttggattac ggtgagccga
     1801 actaggcagt tgtgcttaga tggatcggtt cgctactgct gcaggccggg aaagcatgcc
     1861 tgatttttgt tgacgcaaaa tgaggatgtg cggatatacc tgagcgagtt actcgggtat
     1921 acacttcgaa aagactgttg cggatccgca gcactgcagt ttttcaaaaa caagaaggaa
     1981 actcccagat gactcaatcc cccgacgaag ccaggccccg cgtcaagggt tcccagaccg
     2041 ccatggcgg
//
LOCUS       DBMDTI010000340         2025 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_12148_length_2025_cov_2.558212, whole genome shotgun sequence.
ACCESSION   DBMDTI010000340 DBMDTI010000000
VERSION     DBMDTI010000340.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12148_length_2025_cov_2.558212"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="ACZPB6_10375"
     CDS             complement(<1..855)
                     /locus_tag="ACZPB6_10375"
                     /inference="COORDINATES: protein motif:HMM:NF036772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HIR1184905.1"
                     /translation="MTDSARPFEGLALAVAAPSRQWVTNEGGIDWALRRRAFSAAEAL
                     GFRVRELEGTYLEEARFAGPDAVRAGDLTCALTELDADLVMAMRGGYGCGRILDMLDW
                     ERIGAASTPFVGFSDVTAFELALLARTGRTSWQGPTLRDLIDPDPLTIEGLLAVVGRR
                     PFASTWQSDDTDDADAEGVLWGGNLAMIASLAGTPWMPQVENGILFIEDVAEPAYRIE
                     RMLLTLDMAGILRRQQAVVVCDLQGADRPAGWEGDFRLSHALDFMRARTGLPFVTGCP
                     FGHLHRKLS"
     gene            complement(852..1793)
                     /gene="tadA"
                     /locus_tag="ACZPB6_10380"
     CDS             complement(852..1793)
                     /gene="tadA"
                     /locus_tag="ACZPB6_10380"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR1184906.1"
                     /translation="MYSLIVRDYSIADIEAFSALSIRRGSTIASLAKASSDNLRIVTG
                     AWLLFLPKAADEAAVRASAERFLAGPGAWMNETPEGLVAAALESGLLEKTFEGFANGL
                     APRPNVTAARLTDELETAAAILAARRARTREALQAKNRAVNSGEPPVDDEADRRFMTE
                     ALAEARAAAQAGEVPVGAVLVADGRIIACAGNRTLRNGDPTAHAEMLVLREGARVMKN
                     HRLAETTLYVTLEPCPMCAGAIVQARPGRIVFGATDERMGAVGGALSLFELPGVNHRP
                     WVRRGVMAQEAKTLLADFFAARRGAKENEREGEGEAR"
BASE COUNT          335 a          701 c          652 g          337 t
ORIGIN      
        1 cgagagcttc ctgtgaaggt gcccgaaggg acagcccgtc acgaagggaa gccccgtgcg
       61 agcccgcatg aagtcgagcg catgcgagag cctgaagtcg ccttcccagc ctgcgggcct
      121 gtccgcgccc tgcaggtcgc agacgacgac cgcctgctga cgcctcagga tccccgccat
      181 gtcgagcgtg agcagcatgc gctcgatcct gtaggcgggc tcggccacgt cctcgatgaa
      241 gagaatgccg ttttcaacct gaggcatcca gggcgtgccc gccagcgagg cgatcatcgc
      301 cagattgcca ccccaaagga cgccctcggc atctgcatcg tccgtgtcgt ccgactgcca
      361 cgtcgatgcg aagggcctgc gaccgacgac ggccagaagc ccctcgatcg tgaggggatc
      421 aggatcaatc aagtccctga gcgtcgggcc ctgccagctc gtgcggcccg tccttgccag
      481 aagcgcgagc tcgaaggccg tcacgtccga aaagccgacg aagggcgtag acgcggcacc
      541 gatccgctcc cagtcgagca tgtcgagaat gcgtccgcac ccatagccgc cgcgcatcgc
      601 catgacgaga tccgcatcga gctctgtgag cgcacaggtc agatccccgg ccctgacggc
      661 gtcgggacct gcaaagcgcg cctcctcgag atacgtgccc tcaagttcgc gcacgcgaaa
      721 gccgagcgct tcggcggccg agaacgcccg gcgcctgagc gcccagtcaa tgccgccctc
      781 gttcgtcacc cactgtcgcg acggcgccgc gacggcaagc gccagtccct cgaagggcct
      841 tgcggaatcg gtcatcgggc ttctccttcg ccttcccttt cgttttcctt cgcgccgcgg
      901 cgcgcggcga agaaatcggc gagcagcgtc ttcgcctcct gcgccatgac gccgcgcctc
      961 acccacgggc ggtggttgac gcccggcagc tcgaagagcg agagtgcgcc gcccacggcg
     1021 cccatgcgct cgtcagtcgc gccgaagacg atgcggccgg gtcttgcctg cacgatcgcg
     1081 cccgcgcaca tcgggcaggg ctcgagcgtg acgtagagcg tcgtctcggc aagcctgtgg
     1141 ttcttcatga cgcgggcgcc ctcgcgcagc acgagcattt cggcgtgcgc cgtcggatcg
     1201 ccgttgcgaa gcgtgcggtt gcccgcacac gcgatgatcc tgccgtccgc aacaagcacg
     1261 gcgcccacgg gcacctcgcc cgcctgcgcg gcggccctcg cctcggcgag cgcctcggtc
     1321 atgaagcggc ggtcggcctc gtcgtcgacg ggcggctctc cgctgttgac agcgcggttc
     1381 ttcgcctgca gcgcctcgcg cgttcgggcg cgtcgtgcgg caaggatcgc cgcggcggtc
     1441 tcgagctcgt cggtgagcct tgcggccgtc acgttcgggc gcggcgcgag tccgttggca
     1501 aagccttcga acgtcttctc aagaaggccc gactcaaggg cggcggcaac aagtccctcg
     1561 ggcgtttcgt tcatccaggc gccgggcccc gcaagaaagc gctctgcgga tgcgcgaacc
     1621 gccgcctcat ccgccgcctt cggcagaaag aggagccacg cgcccgtgac gatgcgcaga
     1681 ttgtcggagg aggccttcgc gaggctcgcg atcgtgctgc ccctgcggat ggagagcgcg
     1741 ctgaacgcct cgatgtcggc gatggaatag tccctgacga tgagagagta catcttcgtt
     1801 ctcacctttt ttcgtgtcga atattgacgg gaaagcgccc gctgaggagc gcgcgcgtag
     1861 aatgttcccg atttgaagaa agtccgtttg gaacctcatc acaaggattc atgatcgtga
     1921 agaacaagct tgttgcgggc gccctggcgc tctccctttt tgcgactccc attgtagcgg
     1981 ttcaggccgc ctccacttcg gacacaagcg aagcgcttgc cgagt
//
LOCUS       DBMDTI010000341         2013 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_12230_length_2013_cov_3.392259, whole genome shotgun sequence.
ACCESSION   DBMDTI010000341 DBMDTI010000000
VERSION     DBMDTI010000341.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12230_length_2013_cov_3.392259"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            140..586
                     /gene="dut"
                     /locus_tag="ACZPB6_10385"
     CDS             140..586
                     /gene="dut"
                     /locus_tag="ACZPB6_10385"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR1184907.1"
                     /translation="MQIDVKILDERMRERMPAYATPGSAGLDLRALLDEPLVINPGET
                     VLVRTGLAIHIGDPGYAALILPRSGLGHKKGIVLGNLVGLIDSDYQGELMISTWNRGQ
                     APFTLEPFERLAQLMIVPVMQADFRVVDEFDESERGEGGFGSTGRG"
     gene            complement(653..1162)
                     /locus_tag="ACZPB6_10390"
     CDS             complement(653..1162)
                     /locus_tag="ACZPB6_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HIR1184908.1"
                     /translation="MTEINELCRDIQMKLPDEIWMLDSLERYRRGAVHTDDFARMDGR
                     TYLFGVVEVPLAYEDDASFTWGCWIEVDRSLHDAYLEAFQSPAADRLTGDGRLANDIP
                     GYEDAAGARVEVMFSSERRPVFVVDASNSLGRDQRSGLSLEDHQALDDILFGDDEDED
                     DEQDWNERD"
     gene            complement(1162..>2013)
                     /locus_tag="ACZPB6_10395"
     CDS             complement(1162..>2013)
                     /locus_tag="ACZPB6_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1184909.1"
                     /translation="PVGSFLFTGPTGVGKTEVARRLAGALGVQLLRFDMSEYAESHTI
                     SRLIGSPPGYVGHGEGGLLTEQVTKHPYSVVLLDEMEKAHPSLFNLMLQVMDHGKLTD
                     ASGREADFRNVVLIMTSNVGAREMERNTIGFGGSRTGDDGAAIRMAFTPEFRNRLDAV
                     VRFAPLSVDSIASVVDKFLAELSDQLRARGVEPVYSADFRAWLARNGYDPHMGARPMR
                     RLIADKVRRPLAEELLFGRLAGGGKVVFDIRTDAGGEEVVFEIPAADYNDQKPSRLSE
                     DGPEGEA"
BASE COUNT          400 a          667 c          576 g          370 t
ORIGIN      
        1 agccccgaca actgcgtgct ccttgtaacg ccagagagcg ccgaagcctt cgggcctgcc
       61 tccaagcggg aggttgcact gaaaatcgtc agccgcatcg cctccatgct caattctcaa
      121 acctcactca ttcagaacca tgcagattga cgtcaaaatc ctcgacgaac gcatgcgcga
      181 acgcatgccc gcctacgcca cgcccggcag cgcaggcctc gacctccgtg cccttcttga
      241 cgagcctctc gtcatcaatc ccggtgaaac cgtgctggtg cgcacaggcc tcgccatcca
      301 catcggagat cccggctatg ccgccctgat cctcccacgc tcggggctcg gtcacaaaaa
      361 aggcatcgtg ctcggcaatc tcgttggtct catcgacagc gactatcagg gcgagctgat
      421 gatctcgacc tggaaccgcg gtcaggcacc cttcacgctc gaacccttcg aacgcctcgc
      481 tcagctcatg atcgtgcccg tcatgcaggc ggacttccgc gtggtcgatg agttcgacga
      541 aagcgaacgc ggcgaaggcg gcttcggatc cacgggcagg ggctgacctc tctccaaatg
      601 aaacaggctt ccacacggaa gcctgtttca tttcagtgat ctcaaacaaa cgtcagtcgc
      661 gctcgttcca gtcctgttcg tcgtcctcat cctcatcgtc gccgaaaagg atgtcgtcga
      721 gcgcctgatg atcctcaagc gaaagaccgc tcctctggtc ccgcccgaga gagttgctcg
      781 cgtccacgac gaagacaggg cggcgttcgg acgagaacat cacttcaacg cgggcgcctg
      841 cagcatcctc ataccccggg atgtcgttgg caagcctccc atcgcccgtc aggcggtcgg
      901 cggcaggact ctggaaagcc tcgagatatg cgtcgtgcag agaacggtcg acctcaatcc
      961 agcaacccca tgtgaagctc gcatcgtctt cgtaggcgag cggaacctca accacgccga
     1021 aaagataggt gcgaccgtcc attctggcaa agtcatcggt atggacggcg ccgcggcggt
     1081 agcgctcgag cgaatccagc atccagatct cgtccggaag cttcatctga atgtcgcggc
     1141 agagctcgtt gatttcagtc atcaggcttc tccttcagga ccgtcctccg acaaacggga
     1201 cggcttctgg tcattgtagt cggcggcagg gatttcaaac accacttcct caccgcccgc
     1261 atcggttcgg atgtcgaaga cgacctttcc accacccgcc aggcggccga acagcagctc
     1321 ttcggccagc ggacggcgga ccttgtcggc aatcaggcgg cgcatcggac gcgcccccat
     1381 gtgcgggtcg taaccgtttc tggccagcca ggcgcggaag tccgccgaat agacgggctc
     1441 gacacctctg gctctgagct gatcggaaag ctcggcaagg aatttgtcca cgacggacgc
     1501 aatggaatcc accgaaagcg gcgcgaagcg caccacggcg tcgagtcgat tgcggaactc
     1561 gggcgtgaag gccatcctga tggccgcgcc gtcatctccc gtcctgcttc cgccgaaccc
     1621 gatggtgttg cgctccatct ctcgggcgcc cacattgctg gtcatgatga gcaccacgtt
     1681 tcggaaatcc gcctcgcggc cggaggcgtc ggtgagcttg ccgtggtcca tgacctgcag
     1741 catcaggttg aagagcgacg ggtgcgcctt ttccatttca tcgagcagca cgacgctgta
     1801 gggatgcttc gtcacctgct cggtgagaag cccgccctcg ccgtgtccga catagcctgg
     1861 aggcgagccg atgagacggc tgatcgtatg cgattccgcg tactcggaca tgtcgaaacg
     1921 aagaagctgt acgccgagcg ctcccgcaag cctgcgggcc acctcggtct tgccgacgcc
     1981 cgtcggcccc gtgaagagga aggagcccac ggg
//
LOCUS       DBMDTI010000342         2012 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_12241_length_2012_cov_3.114076, whole genome shotgun sequence.
ACCESSION   DBMDTI010000342 DBMDTI010000000
VERSION     DBMDTI010000342.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12241_length_2012_cov_3.114076"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            424..1452
                     /gene="purM"
                     /locus_tag="ACZPB6_10400"
     CDS             424..1452
                     /gene="purM"
                     /locus_tag="ACZPB6_10400"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR1184910.1"
                     /translation="MSQLSYAAAGVSIDAGDELVERIKPFAKRTMIPGVLGSIGGFGA
                     LFEVSKEYKNPVLVTGTDGVGTKLMLAFKLGRHDTVGQDLVAMSVNDILVQGARSLFF
                     LDYYACGKLDVDVAGRVVAGVAKGCELAGCALIGGETAEMPGMYPEGEYDLAGFAVGV
                     VEKDQIIDGRTIQPGDTILGLASSGPHSNGFSLIRKVVEVAGADWNMPFDGATLADRA
                     MEPTRIYVKQVLEVMKSVKIKGMAHITGGGLVENVPRVLPEGVQAVIDGKSWTRPAIF
                     DWLQEKGNIDSHEMHRVFNNGIGLVVIVSAEDAEKARGAFEAQGEKVFTIGTVAKLPE
                     GEAPCVVR"
     gene            complement(1518..>2012)
                     /locus_tag="ACZPB6_10405"
     CDS             complement(1518..>2012)
                     /locus_tag="ACZPB6_10405"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-dimethylallyltransferase"
                     /protein_id="HIR1184911.1"
                     /translation="TAARLAPNDSQRIGRALEVWKMTGRPISAFHAESVRRPAVETLT
                     VGLLPSDRKWLHARIEARFDQMLADGFLDEVRSLMMRPDYDPDSPAMRAVGYRQAIDF
                     IEGRTDMAAFYLAGVAATRQLAKRQMTWMRSMPDVALLDPLDAGALDSVLGLLEKVTS
                     PALV"
BASE COUNT          386 a          676 c          546 g          404 t
ORIGIN      
        1 cctgcggcca cttctcgcag aacactgacg gcttcgccgt tggcaccgac aacgaagttg
       61 tcgagcgtcg gcttgtcgcg ctcctgcagg tcgagcagat actgatcggg ttcgtgttcg
      121 tacataatct cgggcggtgg aaatctgagc gcatgatgcg cacacgtcca gaatcttaga
      181 gtttacctcg aaaacattgt tttcgtccgc tccatttgtc atgcctccgc ttcactcaaa
      241 cccttgctcc gcctgcgacc tcccctgctc aaaaatcgtc ttatccgcct catgagacgg
      301 ctctttggct tatactttcg caaactcttt tattaggcaa cccgtccccg gtcacaccct
      361 tcggcacgac gtgccccctc ttctgcgtgt gcccgcggac cctctttact cgagatactt
      421 tccatgagcc aactttccta cgcggctgcc ggcgtttcca tcgatgccgg cgacgaactc
      481 gttgaacgca tcaagccctt tgccaagcgc acgatgatcc cgggcgtcct cggctccatc
      541 ggcggcttcg gcgcgctctt cgaagtcagc aaggaataca agaacccggt tctcgtcacc
      601 ggcacggacg gcgtgggcac caagctcatg ctcgccttca agctcggccg tcacgacacg
      661 gtcggccagg acctcgttgc catgagcgtc aacgacatcc tcgtgcaggg tgcccgcagc
      721 ctcttcttcc tcgactacta tgcctgcggc aagctcgacg ttgacgtcgc cggccgcgtt
      781 gtcgctggtg tcgccaaggg ctgcgaactc gcaggctgcg ctctgatcgg cggcgaaacc
      841 gccgaaatgc cgggcatgta tccggaaggc gaatacgacc tcgcaggctt cgccgtcggc
      901 gttgtcgaaa aggaccagat catcgacggc cgcacgatcc agccgggcga cacgattctc
      961 ggccttgcct cgagcggccc gcactccaac ggcttctctc tcatccgcaa ggtggtcgaa
     1021 gtcgccggcg ctgattggaa catgccgttc gacggtgcca cgctcgccga ccgcgccatg
     1081 gaacccaccc gcatctacgt caagcaggtt ctcgaagtca tgaagtccgt gaagatcaag
     1141 ggcatggccc acatcacggg cggcggcctc gtcgagaacg tgccccgcgt tcttcccgaa
     1201 ggcgttcagg ccgtcatcga cggcaagtcc tggacgcgcc cggccatctt cgactggctc
     1261 caggaaaagg gcaacatcga cagccatgaa atgcaccgcg tcttcaacaa cggcatcggt
     1321 ctcgtcgtga tcgtctccgc cgaagacgcc gaaaaggccc gcggcgcctt tgaagctcag
     1381 ggcgaaaagg tcttcacgat cggcacggtc gccaaacttc ccgaaggtga agctccctgc
     1441 gtcgtccgct gatcggcctt aggccactga ccaagaattg cggcactgca acagatgcag
     1501 tgccgctttc tttttattca aacaagcgca ggagaagtca ccttctccag cagtccgagc
     1561 acgctgtcga gcgcgcccgc gtcaagtggg tccaacagag caacgtcggg catgctgcgc
     1621 atccaggtca tctgtctctt ggccaactgt cgggtggccg caacgcctgc cagataaaag
     1681 gccgccatat cggtgcgccc ctcgatgaaa tcaattgcct gtcgatagcc tacggcccgc
     1741 atggccgggc tgtcaggatc atagtctgga cgcatcatca gagaccttac ctcatccaga
     1801 aagccatcgg caagcatctg atcaaacctt gcctcgattc gggcatgcag ccactttcta
     1861 tccgagggca gcagccccac cgtcaacgtc tccaccgcag gtctgcgaac gctctctgca
     1921 tggaaggccg agatcggcct gcccgtcatt ttccagacct ccagagcgcg cccgatgcgc
     1981 tggctgtcgt tgggcgccag tctggcggcc gt
//
LOCUS       DBMDTI010000343         1986 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_12440_length_1986_cov_2.879045, whole genome shotgun sequence.
ACCESSION   DBMDTI010000343 DBMDTI010000000
VERSION     DBMDTI010000343.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12440_length_1986_cov_2.879045"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="ACZPB6_10410"
     CDS             complement(<1..836)
                     /locus_tag="ACZPB6_10410"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR1184912.1"
                     /translation="MLDLTMLRKQLPEVVARLATRNFVFPEKEFNELEAARKTVQTKT
                     EDLQQKRNVLSRQIGMAKKNGEDASALMAEAAQIPEELTKLEAELDDIRTRLNDMLLR
                     IPNLPHESVPVGKDESENVEVRRWGTPREFDFEVKDHVDVGAPLGLDFDTAAKESGAR
                     FAFMRGQIARLHRALAQFMLDTHTRENGYVECYTPYIVTASTMQGTGQLPKFEEDLFA
                     AKKGGAFGEQEQMYLVPTAEVTLTNQVAGMMLSYKDLPLKVTAHTPCFRSEAGAYGRD
                     TRG"
     gene            complement(956..>1986)
                     /locus_tag="ACZPB6_10415"
     CDS             complement(956..>1986)
                     /locus_tag="ACZPB6_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR1184913.1"
                     /translation="RPTLLFVDEVHRFSKSQQDAFLPHVESGLFVFVGATTENPSFEV
                     NSALLSRSTVYQLQSLKPEESEVLLERALEREFPKLSVTEDARRVMIELSDGDARRLL
                     NAVDVSCTMALERGMTEVDAGFLRKTLPATLRRFDKGGDNYYDQISAMHKSVRGSDPD
                     AALYWMCRMLDGGCDPRYIARRVVRMAIEDISLADPRATQLAVEAAEIYERLGSPEGE
                     LAIAQAVVYLACAPKSNAVYNAYNAVRAFVKTDRTRPVPMYLRNAPTKLMKELGYHEG
                     YRYAHDEPGAFAAGEIYMPEGLEGMKWYKPVDRGLEIKIAEKLARLESLNEEARKAGR
                     GRRRGQGR"
BASE COUNT          386 a          591 c          592 g          417 t
ORIGIN      
        1 ccgcgggtgt cgcggccgta agcaccggct tcggaacgga agcacggcgt atgggccgtc
       61 accttgagcg gaaggtcctt gtagctgagc atcatgccgg caacctgatt cgtcagcgtg
      121 acttcagcag tcggcacgag atacatctgt tcctgttcac caaaggcacc gcccttcttg
      181 gcggcgaaga ggtcctcctc gaacttcggt agctggccgg tgccctgcat cgtggaagcc
      241 gtcacgatgt acggcgtgta gcactcgaca tagccgtttt cacgggtgtg cgtgtccagc
      301 atgaactggg cgagggcgcg atgaagacgg gcaatctgac cgcgcatgaa ggcgaagcgg
      361 gcgccggact ccttggcggc cgtatcgaag tcgaggccga gcggagcgcc cacgtcgaca
      421 tggtccttga cttcaaagtc aaactcgcgc ggggtgcccc aacggcggac ttcaacattt
      481 tcagactcat ccttgccgac cggcacgctt tcgtgcggga ggttaggaat gcggagaagc
      541 atgtcgttga gacgcgtgcg aatgtcgtcg agttcggcct caagtttcgt gagttcttcg
      601 ggaatctggg cggcttcagc catcagggcg gaagcgtcct caccgttctt cttggccatg
      661 ccgatctgac gggagagcac gttgcgcttc tgctgcagat cttcggtctt ggtctgcacg
      721 gtcttgcggg cagcttcaag ttcgttgaat tccttttcag ggaacacaaa gttgcgcgtc
      781 gcaaggcggg caaccacttc agggagctgc ttgcgcagca tcgtcaggtc aagcattgtt
      841 tttaccttta aagttgccgg cgcaagcacg ccggccatca gaagtcgatc ttttaatttt
      901 ctccttttat cggagaaaag gcaaattcag cagtactgcg gctcatgagg accacctacc
      961 tgccctggcc ccttctgcgc ccgcgccccg cctttctggc ctcttcgttg agcgactcca
     1021 ggcgagcaag cttttcagcg atcttgatct ccagaccgcg atcgacaggt ttgtaccact
     1081 tcatgccttc gagcccttcg ggcatgtaga tctcgccggc ggcaaaggcg ccaggctcgt
     1141 catgtgcgta gcgatagccc tcatggtatc cgagctcctt catgagcttc gtaggcgcgt
     1201 ttcgcaaata catggggacg ggacgcgtgc ggtccgtctt cacgaaggcc cgcactgcgt
     1261 tgtaggcgtt gtagacggcg ttgctcttgg gtgcgcaggc aagatagacg acagcctgcg
     1321 cgatggcaag ctccccctcg ggagagccga gtcgctcgta gatttcagcc gcctccacgg
     1381 caagctgcgt cgcgcgcgga tcagccagcg agatgtcctc gatcgccatg cgcacgacgc
     1441 ggcgggcaat gtagcgcgga tcgcacccgc cgtcgagcat gcggcacatc cagtaaagcg
     1501 ctgcatcggg atcggatcca cgcacggact tgtgcatggc gctgatctga tcgtagtagt
     1561 tgtcgccgcc cttgtcgaag cggcgcagag ttgcaggaag cgtcttcctg agaaatcccg
     1621 catcgacctc cgtcattccc ctctcgagag ccatcgtgca ggacacgtcg accgcattga
     1681 gaagccgcct ggcgtctccg tcggacagtt cgatcatgac acggcgtgca tcctccgtca
     1741 ccgagagctt cggaaactct ctctcaagcg cacgctcaag aaggacttcg gactcctcgg
     1801 gcttgagact ctgaagctga tacacagtcg aacggctcag aagagccgaa ttcacctcaa
     1861 agctgggatt ttcggtcgtc gcgccgacaa agacgaagag tcccgactcc acatgaggca
     1921 gaaaggcgtc ctgctggctc ttgctgaatc gatggacctc atccacaaaa agaagagttg
     1981 gcctgc
//
LOCUS       DBMDTI010000344         1978 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_12511_length_1978_cov_1.950986, whole genome shotgun sequence.
ACCESSION   DBMDTI010000344 DBMDTI010000000
VERSION     DBMDTI010000344.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12511_length_1978_cov_1.950986"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            130..492
                     /locus_tag="ACZPB6_10420"
                     /pseudo
     CDS             130..492
                     /locus_tag="ACZPB6_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013933.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            612..1925
                     /locus_tag="ACZPB6_10425"
     CDS             612..1925
                     /locus_tag="ACZPB6_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR1184914.1"
                     /translation="MLEGIVLGLFAAAILSCALTGHTVIWALIAGFFLFSGYGLFRGH
                     GLKALGAMASKGVRTIMGIVIVMALIGMLTGSWRASGTIARLVTDAASFITPEWGLLA
                     AFLLNGLLSTLTGTAFGTVATMGVITATMLDAMGVSPLLSGGAILAGVFMGDRCSPVS
                     TSAALVAVVTKTNLYTNLKLMVKTSVVPLLAACAVYAAIGLLNPGRDAGIDVTGIFRS
                     AFSLHWTTYLPAVVLLALIFLKFSIRLTMVASTLAAVLLCAFVQDMPWRTIGETLVLG
                     FAPEDPGVAKMLAGGGLVSMLPVCAIILISSTYSGLLEGTGLIARLKGLLPGVARRLT
                     PFGAVLTTAVVTCGIACNQALPVMLTNQLCDEVRPDRQKMAIDLENTVIVIAPLIPWS
                     IAGAVPIASVGAPAACVLAAFYLWFLPLWQWILAFVERARQTREA"
BASE COUNT          315 a          687 c          576 g          400 t
ORIGIN      
        1 ttcgacgatg taggaagagg tggaggtgcg cataaggcaa tgttacttca cgttcgaaaa
       61 ctttgtgcgc cttacctctc attgaaatga gaggtaaggc gcacgggttc tgtcaagacc
      121 tggcggaagg tcccgccgaa gatcgtgatc gatgcgccga gcgtgcaggt gcccgacaac
      181 gccggcgagg ggctcttttc gaagtgcttc atcctcgccg tcacgaatcc gcagccgatg
      241 atcttctgtg tctcgctcat gccgcagttc atcgacccgg agatcgccta catcccgcag
      301 gtaaccttca tgatcgcggt ctacgcgctg atggtcttcg tctgcatgat tttttacgcg
      361 ctcatcgccg accgcatgcg cgtcttcctc gcaaggggca gcggcccgaa gctcatgaac
      421 aaggcaagcg ccgtgatctt catgctgctc gcctgctggg tgctctggaa cacggcgcag
      481 ccgttcctct gagacaccca caaagaggtg tttccaaagg cgcccgcctt tggcacaatg
      541 ctcacttgaa tgtccagccc ggcgcattcc gcgtccgggc cgctctaatt tgaagatacg
      601 cacacctcat catgcttgaa ggcatcgtcc tcggcctctt cgccgccgcc atcctctcct
      661 gcgcactcac gggccatacg gtcatctggg cactcatcgc aggcttcttc ctcttctcgg
      721 gctacgggct cttccgcgga cacggcctca aggcgctcgg cgccatggcc tcgaagggcg
      781 tgcgcaccat catgggcatc gtgatcgtca tggcgctcat cggcatgctc acgggctcgt
      841 ggagggcttc gggcacgatc gcccgtctcg tcacggatgc ggcgagcttc atcacgcccg
      901 aatggggact tcttgcggcc ttccttctca acggtcttct ctccacgctg acgggcacgg
      961 ccttcggcac ggtcgcgacg atgggcgtca tcaccgccac gatgctcgac gccatgggcg
     1021 tgagcccgct cctttcgggc ggcgccattc tcgcgggcgt cttcatgggc gacaggtgct
     1081 cccccgtctc gacgagcgcc gcgctcgtcg ccgtcgtcac gaagacgaac ctctacacca
     1141 acctgaagct gatggtgaag acgagcgtcg tgccgcttct ggccgcatgc gccgtctatg
     1201 cggccatcgg ccttctcaat cccggcaggg atgccggcat cgacgtcacg ggcatcttcc
     1261 gcagcgcctt ctcgctccac tggacgacct atctgcccgc cgtcgtgctc ctcgcactca
     1321 tcttcctgaa gttctcgatc cgcctgacca tggtggccag cacgctcgct gcggtcctgc
     1381 tgtgcgcgtt cgtccaggac atgccctggc gcacgatcgg cgagacgctc gtgctgggct
     1441 ttgcgcccga ggacccgggg gtggcgaaga tgctcgcggg cggcggcctc gtctcgatgc
     1501 tccccgtctg cgcgatcatc ctgatctctt cgacctattc gggcctgctc gagggcacgg
     1561 gcctcatcgc ccgtctcaag ggactgctgc ccggcgtcgc gcgccgccta acgccctttg
     1621 gcgccgtcct gacgacggcc gtcgtcacct gcggcatcgc ctgcaaccag gccctgcccg
     1681 tgatgctcac gaaccagctc tgcgacgagg tccgccccga ccgccagaag atggcgatcg
     1741 accttgagaa caccgtcatc gtgatcgcgc cgctcatccc gtggtcgatc gcgggcgccg
     1801 tgcccatcgc ctcggtcggc gcacccgcgg cctgcgtgct cgcggcgttc tacctctggt
     1861 tcctgccgct ctggcagtgg atcctcgcat tcgtcgagcg tgcgcggcag actcgcgaag
     1921 cctgatccag acgacctctg acaccgccgg actcaaaaaa aaaaaaaaaa tggagcac
//
LOCUS       DBMDTI010000345         1960 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_12642_length_1960_cov_2.026358, whole genome shotgun sequence.
ACCESSION   DBMDTI010000345 DBMDTI010000000
VERSION     DBMDTI010000345.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12642_length_1960_cov_2.026358"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="ACZPB6_10430"
     CDS             <1..586
                     /locus_tag="ACZPB6_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184915.1"
                     /translation="SAYGLYVLNLRVSVPVESLIELGQEALLLSAAIITLRTASLEKA
                     LSGGLWLVGGFLVALFIRELDGWIGHAWVYCELVWLCGVAWAVKAAGLGTVIPGLAHF
                     IGHRAFPIMCAGVAILLVYSRLYGMQTLWLAYAGEACSGLYSVKTLSEESTELLAYMM
                     IFTSAAFYAGNCLRMRRRRARAERLQKEAGIKTA"
     gene            complement(706..1485)
                     /locus_tag="ACZPB6_10435"
     CDS             complement(706..1485)
                     /locus_tag="ACZPB6_10435"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1184916.1"
                     /translation="MPDHIQPLAVITGASSGIGRELAKLAAQKGYRLLLIARNMRALE
                     VLSRDLRTETHLLALDLTAAGAVDQISSWLSDRQIVPEIFINNAGFGAFGPASDMDEA
                     MLKKMIRLNVMALTSLSLRMARRMKREGRGRIMNVASTAAFQPCPYLGVYGATKSYVL
                     SFTESLAEELKGSGVTVTAFCPGPTRTQFGVTAGLSQESPFDRLSADPERVAETGFKG
                     MMAGRVVVLDGAANRFGAFMTRFLPRCLVRRIAGGMLAKLK"
     gene            1622..>1960
                     /locus_tag="ACZPB6_10440"
     CDS             1622..>1960
                     /locus_tag="ACZPB6_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YczE/YyaS/YitT family protein"
                     /protein_id="HIR1184917.1"
                     /translation="MTTPIERPNGTDSGKNLLAQILLLLTGMAVHTLGIALITRADLG
                     TTPISTLPLTLSAVMGVTLGTSTFIINIAFFLSQIVMLGSRFERRQWLQLPSVLLFSA
                     MIDAWMAVLSR"
BASE COUNT          341 a          637 c          612 g          370 t
ORIGIN      
        1 gtccgcctac ggcctctacg tcctgaacct cagggtaagc gtccccgtcg agagtctgat
       61 cgagctgggc caggaagcgc tgctcctttc ggcggcgatc atcacactca ggaccgcgtc
      121 gcttgagaag gcgctctcgg gcgggctgtg gctcgtcggc ggctttctcg tcgcgctctt
      181 catccgggag ctcgacggtt ggatcggcca cgcctgggtc tactgcgagc tcgtctggct
      241 ctgcggtgtc gcctgggccg tgaaggctgc ggggctcggc accgtcattc cgggacttgc
      301 ccacttcatc ggccaccgcg cctttccgat catgtgcgcg ggcgtagcga tcctgctcgt
      361 ctactcgcgc ctctacggca tgcagacgct ctggctcgcc tatgcgggcg aggcctgctc
      421 gggcctttat tcggtgaaga ccctctcgga agaatccacc gaacttctcg cctacatgat
      481 gatcttcacg agcgccgcct tctatgcagg caactgcctg cgcatgcgcc gccgcagggc
      541 ccgcgccgaa cgcctacaga aggaagccgg catcaagacc gcctgacaag gccgcagccc
      601 gcaaaaagga agggacccaa atcctgatgg acatgggtcc ctttgttcat tccgccttcg
      661 aaagccatcg gggtctttca gaagcgttcg accgtttcgg cgctgttatt ttaacttagc
      721 gagcattccg cccgcgatcc tgcgcacgag gcagcgggga agaaatcggg tcatgaaggc
      781 gccgaagcgg ttggctgcgc cgtcgagtac gacgacgcgg cccgccatca tgcccttgaa
      841 gcccgtttcg gccacgcgtt cggggtcggc ggaaaggcga tcgaaggggc tttcctgcga
      901 gaggcccgcg gtcacaccga actgcgtgcg cgtggggccg ggacagaagg cggtgacggt
      961 gacgcctgag cccttgagct cctctgcgag cgactcggtg aaggagagca cgtagctctt
     1021 ggtggcgccg tagacgccga ggtaggggca gggctgaaag gcggccgtgg aggccacgtt
     1081 catgatcctg ccgcggccct cgcgcttcat gcggcgcgcc atgcgaagcg agaggctagt
     1141 gagcgccatg acgttgagcc tgatcatctt cttcaacatc gcctcgtcca tgtcggacgc
     1201 aggaccgaag gcaccgaagc ccgcgttgtt gatgaagatc tccggcacga tctgccgatc
     1261 cgagagccag ctcgagatct ggtcgaccgc acccgccgcc gtcaggtcga gcgcgagcag
     1321 gtgggtttcg gtcctcaggt cgcggctcag cacttcaaga gcgcgcatgt tgcgggcgat
     1381 gagaagaagc cggtagccct tctgggctgc gagcttcgcg agctcccggc cgatgccgct
     1441 cgaggcgcct gtgatgacgg cgagcggctg aatgtggtct ggcatcgatc tgactcacta
     1501 gaatgcatga tgccgcaagt gtacaacggc ttgcgcccca acgcccgccc cttcgaggcg
     1561 gtaagacgcc aacacgggat agtcccatac ggcaaacaac ctagagacat tgagccatcg
     1621 catgacaacc cccattgagc gcccgaacgg gaccgattcc gggaaaaatc tgcttgcaca
     1681 aatccttctg cttctcacgg gcatggccgt gcacacgctc ggcatcgcgc tcatcacgcg
     1741 ggcagatctc ggcaccacgc ccatcagcac gctgccgctt acgctctcgg ccgtgatggg
     1801 cgtcacgctc gggacgtcca ccttcatcat caacattgcg ttcttcctct cgcagatcgt
     1861 gatgctcggc tcaaggttcg agcgccgcca gtggctgcag ctcccatcgg tccttctctt
     1921 cagcgcgatg atcgacgcct ggatggcggt tctctcccgc
//
LOCUS       DBMDTI010000346         1946 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_12774_length_1946_cov_2.109485, whole genome shotgun sequence.
ACCESSION   DBMDTI010000346 DBMDTI010000000
VERSION     DBMDTI010000346.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12774_length_1946_cov_2.109485"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(7..>1946)
                     /locus_tag="ACZPB6_10445"
     CDS             complement(7..>1946)
                     /locus_tag="ACZPB6_10445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR1184918.1"
                     /translation="KGVKTVSIDPRFVPDAAKADVWLPIRPGTDVALMLCWTKYIMEK
                     DLYDHEFVMRWTNLPYLVNVKTKLLVRAADLGMGDAKTYVVWDKKTNSPKPIAYPWDD
                     ALDPALDGAFTWKGVEYKTGWRLLLERCEPWTLEKAAEVCWLDPKKIEEAIKLYCSGP
                     AGVALGVATDQNENSVEAAMGSVILNALRGNVESPGALMQRNPSTNIVPAGSLATRCS
                     YLLPKGQLKKRLGSNEHKGLMQWDAAQPSAVLEAMKTGKPYPIKVWLERSGNKFVVNG
                     HAQDWIEGTKNVDLIVHMFLYPTSFSCYADILLPATEWLETNMLVECMNWVFARQPVA
                     HVWETEDETLFWSKLIKKCAELGNENARRACDPAFMKKDLAYWDSMEELLDGRLANIG
                     MTWKEFKEKQPFPYMPYDKWNTYYVYKQPDKKTGLPKGFGTPSKKLELYGEVWIELGR
                     TGKPYALDEVPPVSVDYDPLPWYAEPAESPLRDIAKEFPLVLTNGRLPMFHHGTLRNN
                     PYMRELYPVPEAWVNPVDAKKYGVAHDDWIWLESKRGKIRGKCRVTEGIPVGVVYMER
                     FWFPETLNTKTQGWREMNVNMLSKNDAPFNRIVGTITLRGFQIRIRKASEGAPEGVWM
                     KPEQFKAWLPQPTDTTKNPEL"
BASE COUNT          322 a          568 c          651 g          405 t
ORIGIN      
        1 catgatttag agctccggat tcttcgtcgt gtcggtgggc tgcgggagcc aggccttgaa
       61 ctgctcgggc ttcatccaga cgccctcggg cgcgccttcg gacgccttgc ggatgcggat
      121 ctggaagccg cggagcgtga tcgtgccgac aatcctgttg aagggcgcgt cattcttcga
      181 aagcatgttg acgttcatct cacgccagcc ctgcgtcttc gtgttgaggg tttccgggaa
      241 ccagaagcgc tccatgtaga cgacgccgac ggggatgcct tcagtcacgc ggcacttgcc
      301 gcggatcttg ccgcgcttgg actcaagcca gatccagtcg tcatgggcga cgccgtactt
      361 cttcgcatcg acgggattga cccaggcctc gggcacggga tagagctcgc gcatgtaggg
      421 gttgttgcga agcgtgccgt ggtggaacat cggcaggcgg ccgttcgtga gaacgagcgg
      481 gaactccttg gcaatgtcgc gcagcgggct ttccgcaggc tctgcgtacc aaggcagcgg
      541 atcatagtcg accgaaacgg gcggcacttc gtcaagcgcg tagggcttgc cggtacggcc
      601 gagctcgatc cagacctcgc cgtagagctc gagcttcttc gagggcgtgc cgaatccctt
      661 cggaaggccc gtcttcttgt cgggctgctt gtagacgtag tacgtgttcc acttgtcgta
      721 gggcatgtac gggaacggct gcttttcctt gaactccttc caggtcatgc cgatgttggc
      781 gaggcgtccg tcgaggagct cctccatcga atcccagtac gcgaggtcct tcttcatgaa
      841 ggcgggatcg caggcgcgcc tggcattttc gttgccgagc tcggcgcact tcttgatgag
      901 cttcgaccag aagagcgtct cgtcctcggt ctcccagacg tgggccacgg gctggcgcgc
      961 gaagacccag ttcatgcact cgacgagcat gttggtctcg agccattcgg tggcgggcag
     1021 caggatgtcg gcgtagcagg agaaggacgt cggatagagg aacatgtgga cgatgaggtc
     1081 cacgttcttc gtgccttcga tccagtcctg agcgtgtccg ttgacgacga acttgttgcc
     1141 ggagcgttcg agccagacct tgatcggata cggcttgccg gtcttcatgg cctcaaggac
     1201 ggcgctcggc tgtgcggcgt cccactgcat cagacccttg tgctcgttcg agccgaggcg
     1261 cttcttgagc tggcccttcg gaaggaggta cgagcagcgg gtagcgaggg agcctgcggg
     1321 gacgatgttg gtggaggggt tgcgctgcat gagggcgccc ggactctcga cgttgccgcg
     1381 cagggcgttg aggatgacgc tgcccatggc ggcctcgacc gagttctcgt tctggtcggt
     1441 cgccacgcca agcgcgacgc cagcggggcc cgagcagtag agcttgatgg cctcctcgat
     1501 cttcttcgga tcgagccagc agacctcggc cgccttttca agcgtccagg gttcgcagcg
     1561 ttcgagcagg aggcgccagc cggtcttgta ctcgacgccc ttccaggtga aggcgccgtc
     1621 gagggcggga tcgagcgcat cgtcccacgg ataagcgatg ggcttgggcg aattcgtctt
     1681 cttgtcccag accacgtagg tcttggcgtc acccatgccg aggtcggcgg cgcgcacgag
     1741 cagcttcgtc ttcacgttga cgagatacgg caggttggtc cagcgcatca cgaattcatg
     1801 atcgtacaga tccttctcca tgatgtactt ggtccagcag agcataagcg ccacgtcggt
     1861 gcctggacgg atcggaagcc agacgtcggc cttggcggcg tcgggcacga agcgcgggtc
     1921 gatcgagacc gtcttgacgc ctttgg
//
LOCUS       DBMDTI010000347         1937 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_12851_length_1937_cov_1.692266, whole genome shotgun sequence.
ACCESSION   DBMDTI010000347 DBMDTI010000000
VERSION     DBMDTI010000347.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12851_length_1937_cov_1.692266"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1937)
                     /locus_tag="ACZPB6_10450"
     CDS             complement(<1..>1937)
                     /locus_tag="ACZPB6_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024487.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HIR1184919.1"
                     /translation="AGEPGFTTSNPSPKITNAWLYRQLERIYTRATAADLDALQSDAE
                     RLCADLREATAQLAVAKAWLAVKSRLSNGPALSNLFSLAAYMKRAVGRGRKSAAWRRE
                     VNRLLPACQQAVPVWIMMIQDALLNFSTASKFDVIVVDEASQADMTALPLLYMGKKVI
                     VVGDDRQVTPLSVGTSEAAVDALCARHLEGRVKEPKLYDSRLSLYGLVQSMAFPAHML
                     VEHFRSVPEIIGWCSRLSYNGTIRPLRDASSSSLKPAVVPWRTRGTAGDNGVNEEEAQ
                     AVIRLLRAMIHDPAYDGKTFGIIPMRSVHGAQVNRIRRLLVENFDPREIERRRIHCGI
                     SAEFQGDERDVVILSLVDSAAPGLPLRKETDGADGMMKKRWNVAVSRARDQLWLVHSF
                     DPAAQLKPDDLRRSLFDWADGVMSGTIGAAEPFDLPDPEFTGQVLRALRKRGYRVEAG
                     HDAGAWRLDMVVRARGRAAAIECDGSGREDEDAIRRDMERQTVLERAGWKFVRVRSAD
                     WFRRPEKTLERVCEALAALGIAPEPEDCAPQTDLLLAHVRANVERLEAGLDMIVFDKE
                     DEDPVPPVAPVTPADLAPDDAEPVVTTPVQFEAPLMPVEVEKADIMLIPEMEMRDAVR
                     ISLPTLSDVFDDEPATEADAK"
BASE COUNT          284 a          610 c          733 g          310 t
ORIGIN      
        1 ttttggcgtc cgcttcggtg gcgggctcgt cgtcgaagac gtcggagagc gtcgggagcg
       61 agatgcgcac cgcatccctc atctccatct cgggaatgag catgatgtcg gccttctcga
      121 cctcgacggg catgagcgga gcctcgaact gcacgggggt cgtgacgacg ggttcggcgt
      181 cgtcgggcgc gaggtcggcg ggcgtcacgg gggcgacggg cggcacgggg tcctcgtcct
      241 ccttgtcgaa gacgatcatg tcgagtccgg cttcgaggcg ctcgacgttg gccctcacgt
      301 gtgcaagaag caggtccgtc tgcggggcgc agtcctcggg ctcgggcgcg atgcccaggg
      361 cggcgagggc ctcgcagacg cgctcgagcg tcttttcggg gcggcggaac cagtcggcgc
      421 tgcgcacgcg cacgaacttc cagcccgcgc gctcgagcac ggtctggcgt tccatgtcgc
      481 ggcggatggc gtcctcgtcc tcgcggcccg agccgtcgca ttcgatggcg gcggcgcggc
      541 cgcgcgcacg cacgaccatg tcgaggcgcc aggcgcccgc atcgtggccc gcctcgacgc
      601 ggtagccgcg ctttcgcagc gcacggagca cctggcccgt gaattcggga tccggcaggt
      661 cgaagggctc ggcagcgccg atcgtgcccg acatgacgcc gtcggcccag tcgaagagcg
      721 agcggcgcag gtcgtcgggc ttgagctgcg cggcggggtc aaaggagtgc acgagccaga
      781 gctggtcgcg ggcgcgggag acggccacgt tccaacgctt cttcatcatg ccgtcggcgc
      841 cgtcggtctc cttcctgagg ggaagcccgg gcgcggcgga gtcaacgagc gagaggatca
      901 cgacgtcgcg ctcgtcgccc tggaattcgg ccgaaatgcc gcagtggatg cggcggcgct
      961 cgatctcgcg cgggtcgaag ttctcgacga gcaggcggcg gatgcggttg acctgcgcgc
     1021 cgtgcacgct tcgcatcggg atgatgccga aggtcttgcc gtcgtaggcg ggatcatgga
     1081 tcatcgcacg aagcaggcgg atgacggcct gggcctcctc ttcgttgacg ccgttgtcgc
     1141 ccgccgtgcc gcgggtgcgc cagggcacca ccgcgggctt gaggctcgag gacgaggcgt
     1201 cgcgaagcgg cctgatcgtg ccgttgtagg aaaggcggct gcaccaaccg atgatttcgg
     1261 gcacggagcg gaagtgctcg acgagcatgt gcgcggggaa ggccatgctc tgcacgaggc
     1321 cgtagagcga gagcctcgag tcgtagagct tgggctcctt gacgcggcct tcaagatgac
     1381 gggcgcagag cgcgtcgacg gcggcctcgc tcgtgcccac ggagagcggt gtcacctggc
     1441 ggtcgtcgcc cacgacgatc accttcttgc ccatatagag gagcggcagc gccgtcatgt
     1501 cggcctggct cgcctcgtcg acgacgatca cgtcgaactt cgaggcggtg ctgaagttga
     1561 ggagcgcgtc ctggatcatc atgatccaga cgggcacggc ctgctggcag gcgggcagaa
     1621 gcctgttgac ctcgcgcctc caggcggcgc tcttgcggcc gcggccgacg gcgcgcttca
     1681 tgtaggcggc aagcgagaag aggttcgaga gcgcgggacc gttcgagagc ctcgacttga
     1741 cggcaagcca ggccttggcg acggcgagct gggccgtcgc ctcgcgaagg tcggcgcaca
     1801 gacgctcggc gtcggactgc agtgcatcga ggtccgcggc ggtcgcgcgc gtgtagatgc
     1861 gctcgagctg gcggtagagc caggcgttcg tgattttggg cgacgggttc gaggtcgtga
     1921 agccgggctc gcccgcc
//
LOCUS       DBMDTI010000348         1934 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_12874_length_1934_cov_2.314239, whole genome shotgun sequence.
ACCESSION   DBMDTI010000348 DBMDTI010000000
VERSION     DBMDTI010000348.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12874_length_1934_cov_2.314239"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="ACZPB6_10455"
     CDS             <1..588
                     /locus_tag="ACZPB6_10455"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434799.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HIR1184920.1"
                     /translation="IIDEYRKLFVSPKGTRPNIVRLIEEEVALHKKHGGGHIILKCNQ
                     LVDPGIVRELYKASQAGVRIECIVRGICSIRPGIPGVSETITVRSIVGRLLEHARIYY
                     FRHAGNEKMYVGSADLMPRNLNGRIEVLAPVTDPDIRANVLDQIVRPQLADNIHAWEL
                     QADGSYVKQHPAEGEAVYDSQEEIGRVLNLMKRRK"
     gene            785..1447
                     /locus_tag="ACZPB6_10460"
     CDS             785..1447
                     /locus_tag="ACZPB6_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1184921.1"
                     /translation="MKYLIVDDHALVRSALSMMLLDRDPSAEVLTAGDVPTTLELIGR
                     EGETADLLILDLNLPGVEGTELMEEIVRRQPMLKILVLSGLTDRGRIMRVLQLGAAGF
                     VPKSLDTDMLTNAIEFVLRGGVFIPSKLLAESQREGFFSETARSLPHLSSNAPKLTDR
                     QKDVLMLLAKGAPIKRICLELNLSEGTVKTHVAAIYRIFGASNRTEALLAARRAGYSI
                     EI"
BASE COUNT          375 a          716 c          491 g          352 t
ORIGIN      
        1 atcatcgacg aataccgcaa gctcttcgtc tccccgaagg gcacgcgtcc gaacatcgtc
       61 cgcctcatcg aggaagaggt cgcgctgcac aagaagcacg gcggcggcca catcatcctc
      121 aagtgcaacc agctcgtcga cccgggcatc gtgcgcgagc tctacaaggc cagtcaggcc
      181 ggcgtgcgca tcgaatgcat cgtgcgcggc atctgctcga tccgtccggg catccccggc
      241 gtctccgaga cgatcaccgt ccgctcgatc gtcggccgcc tgctcgagca cgcccgcatc
      301 tactacttcc gccatgcggg caacgagaag atgtacgtgg gcagcgccga cctgatgccg
      361 cgaaacctca acggccgcat cgaagtcctc gcaccagtca cggatcccga catccgcgcc
      421 aacgtgcttg accagatcgt ccgtccgcag ctcgccgaca acatccacgc ctgggaactc
      481 caggccgacg gcagctacgt caagcagcat cccgccgagg gcgaggccgt ctacgactcc
      541 caggaagaga tcggccgagt cctcaacctg atgaagcgcc gcaagtgatc cgcgcgcctc
      601 atcccgtcag gcctgtgatc tgaacccgaa gcccgcggtc tcctgccgcg ggcttcgccg
      661 catatccccc atcgggtgcg gcctcacttc acgttttcgg actcgttcga cggacttctt
      721 ctttagtaca atctaaaccc gttgaaaatg tagaaacgct ctgctcccgc agagcctcaa
      781 tcgcatgaag tacctgattg tcgatgatca cgcgctcgtg aggagcgccc tttccatgat
      841 gcttctcgac cgggatccct cggccgaagt gctgacggcc ggcgacgtgc ccacaaccct
      901 tgagctcatc gggcgtgaag gcgagaccgc cgacctgctc attctcgacc tgaacctgcc
      961 cggcgtcgaa ggcacggagc tgatggagga aatcgtccgc cgccagccca tgctcaagat
     1021 cctcgtcctt tcgggcctca ccgaccgcgg ccgcatcatg cgcgtactgc agctcggcgc
     1081 agcgggcttc gtgccgaaat ccctcgacac cgacatgctc acgaacgcca ttgagttcgt
     1141 cctcaggggc ggcgtcttca ttccgtccaa gctgctcgcc gaaagccagc gcgagggctt
     1201 cttctccgaa accgcccgct cgctcccgca cctctcgagc aacgccccca agctcaccga
     1261 ccgccagaag gacgtgctga tgcttctcgc caagggcgcg cccatcaagc gcatctgcct
     1321 cgagctcaac ctctccgagg gcaccgtgaa gacccacgtc gccgccatct accgcatctt
     1381 cggcgcaagc aaccgaaccg aggcccttct tgcagcccgc cgcgcgggct acagcatcga
     1441 gatctgacga tcccgccgca caagccctga ggccccgcag gacgagagtc ccgcggggcc
     1501 tcttcgtctg cgtctcccct tccttctcct tttgcgtctc cgcctctccg cttcccttcc
     1561 cgcgcaggct cctccacgaa ttgtgggttt ttcacaacac tactcctcgc atgtccgcgc
     1621 tacgacactt gtcctaaaaa cacgacattc ttcctcatcc gttcgcacta gttccaaaaa
     1681 cagcacgccg ttccccgtcc gtccgcaacc gcctgcagca gaacgaaact tgaagaacac
     1741 acccggaagt gcgcttttcc ctcgtcctga attccgcctc acctcagaca tttgtaaccg
     1801 ccgcacgaag ttgagcgaac gcgtcctgaa agcggataca ttgaatcgac caagcggtgc
     1861 acttccgcat cccccactca tacggagaac acacatgttc aagaagtctt tcgccgcgct
     1921 tgccgtcctc ggct
//
LOCUS       DBMDTI010000349         1933 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_12883_length_1933_cov_2.501092, whole genome shotgun sequence.
ACCESSION   DBMDTI010000349 DBMDTI010000000
VERSION     DBMDTI010000349.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12883_length_1933_cov_2.501092"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="ACZPB6_10465"
     CDS             complement(<1..349)
                     /locus_tag="ACZPB6_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184922.1"
                     /translation="MCGSDGDGGEAEAFSPAAWHDRCDGQSSFNLTYATEDGTIAKVQ
                     IAPSADTDHRAIRRIQRAMERSRRATNPDNYEAVDVVRHGKRHKSLKVKSGRLQWRFS
                     KRYESLRAELAEIF"
     gene            complement(428..1306)
                     /gene="pepE"
                     /locus_tag="ACZPB6_10470"
     CDS             complement(428..1306)
                     /gene="pepE"
                     /locus_tag="ACZPB6_10470"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HIR1184923.1"
                     /translation="MSLSRRSVMQSAVLGSVAALAGVAGSAQAKIKKPLPAQKVSEKR
                     RVLIQPSSRYHTSGYLDFAGEQYEQLYGSSKHEILFIPYAKVAGTYDGYEKQVQDAFK
                     PFGHKIVSIHRFKDPQKAVREASAIAVGGGNTWALVTRMYEAGIIDQIRDRVNGGIPY
                     CGWSAGGNVACPTLRTTNDMPIAEPPSFNTFGFIPFQTNPHFISAAGTSGLNNETRED
                     RLEEFLWYNKDEEVIGLPEGTALFVTGEHDCEVIGPKDAEALYWFRQAEMGMRLERIA
                     LGTKFDLRKIVPGGKL"
     gene            complement(1755..1928)
                     /locus_tag="ACZPB6_10475"
     CDS             complement(1755..1928)
                     /locus_tag="ACZPB6_10475"
                     /inference="COORDINATES: protein motif:HMM:NF025397.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184924.1"
                     /translation="MIEAAAGDRLKPEDLPVGTHLRIMPNHACPTAAPYEKLLLVNEE
                     GRVTAALDHVRGW"
BASE COUNT          363 a          579 c          614 g          377 t
ORIGIN      
        1 gaaatatctc cgctagttct gcccgcagac tttcatagcg cttcgagaag cgccattgaa
       61 gcctgccgga cttcaccttc agagacttgt gcctcttgcc gtgacgaaca acgtcaacag
      121 cttcgtagtt gtcgggattc gtcgcacggc ggcttcgctc catcgcacgc tggattcggc
      181 gtatggcacg gtgatccgtg tccgcgctcg gcgcaatctg cactttggca attgtcccgt
      241 cctcagtcgc ataggtgagg ttgaaggaac tttgaccgtc gcagcggtca tgccaggcgg
      301 ctggggaaaa cgcctcggct tctcctccat cgccatcact gccgcacaaa aagaaccgcc
      361 cgccgggcgc gagcctcggc gggcgtttcc cgaagtcact gacttcggac tcagagcgtc
      421 atgcgtttca gagcttgccg cccgggacga tcttgcgaag gtcgaacttc gtgccgagcg
      481 cgatgcgctc gagcctcatg cccatctcgg cctggcggaa ccagtaaagg gcttcggcgt
      541 ccttcggacc gatgacttcg cagtcatgtt cgccggtcac gaagagagcg gtgccttcgg
      601 gcaaaccgat gacttcctcg tccttgttgt accacaggaa ctcttcaaga cggtcttcgc
      661 gcgtttcgtt gttgaggccg gacgtgcccg cagcggagat gaagtgcggg ttcgtctgga
      721 acgggatgaa cccgaaggtg ttgaaggacg gcggttcggc gatcggcatg tcgttcgtcg
      781 tgcgcagcgt cgggcaggcg acgttgccgc cggcggacca gccgcagtac gggatgccgc
      841 cgttgacacg gtcgcggatc tggtcgatga tgccggcctc gtacatgcgg gtgacgagcg
      901 cccacgtatt gccaccgccc accgcgatgg cggacgcttc acgaacggcc ttctgcggat
      961 ccttgaagcg atggatcgag acgatcttgt ggccgaaggg cttgaaggcg tcctgaacct
     1021 gcttttcata gccatcgtaa gtgcccgcaa ccttggcata ggggatgaag agaatctcgt
     1081 gcttcgagct gccgtagagc tgctcgtact gctcgcccgc aaaatcgagg tagcccgacg
     1141 tgtggtagcg ggagctcggc tggatgagga cgcggcgctt ttcgctcacc ttctgagcgg
     1201 gaagcggctt cttgatcttg gcctgggcgg aacccgcaac gcccgcaagg gcggcgacgg
     1261 agccgagaac ggcgctctgc atgaccgaac ggcgtgacag cgacatgatt ctctccttga
     1321 tgtcgaaaag gtctccgaat cttggtcggg aatccggact gatggtcacc gtgttcgaca
     1381 cgccacacgc attcgggcgg cccctgttga agcacgtacg tcgagtctac gttgagaccg
     1441 caggccccga caccacggga aatccttacc gctcatggtg ttatccatga ttgtgcatta
     1501 catctcttac cctttccaaa acgcctgtcc gcgaagagct tgcggcgcaa cgccgtcgaa
     1561 aggcgacacg ttgcccgcgt tttcccgatg cctgaaaagg tcggcaacaa cttgcgggaa
     1621 aatcccgact gggggatccc gcccgaaagc gggcgaaaga agaacgtcaa aaagggcgcc
     1681 cgttccgatg agaacggacg ccccgaaggc tgcgcgggcg gtggcccgcg cctcaagacg
     1741 acgaaaagaa ggcgtcacca gccgcgaacg tgatcgagcg cggccgtcac gcgtccctcc
     1801 tcattgacga gaaggagctt ttcatagggc gccgcggtcg ggcaggcgtg gttgggcatg
     1861 atgcgaagat gcgtgccgac gggcaggtcc tcgggcttga gtcggtcgcc tgcggcggcc
     1921 tcgatgatgc cgt
//
LOCUS       DBMDTI010000350         1924 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_12957_length_1924_cov_2.359298, whole genome shotgun sequence.
ACCESSION   DBMDTI010000350 DBMDTI010000000
VERSION     DBMDTI010000350.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12957_length_1924_cov_2.359298"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(58..309)
                     /locus_tag="ACZPB6_10480"
     CDS             complement(58..309)
                     /locus_tag="ACZPB6_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184925.1"
                     /translation="MRRSILLLFSLGVIAAGASAGELRTDIFGNVYDGDWSIPVGQVE
                     PGGEGRLRVDVFGDIYVEDRPFPVGHTDHPNLPIFSPSS"
     gene            553..1422
                     /locus_tag="ACZPB6_10485"
     CDS             553..1422
                     /locus_tag="ACZPB6_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184926.1"
                     /translation="MQKILPAVLMLCALAAGSQPVCAATDNQLMREEITAGMTDPAVI
                     EGSADAFGFSGEKAEAYADFIRDLAASRLFLDHVVEQFKKAGIDGAKLRAVHDHGTSD
                     ELAALSEEILQGSLLLTQRLMAEGVPYSSPKVMEAEMRTTKALCGRLSAKACRDLNLV
                     GAQGLAYDDLDTAARTLTADMSEEDVRKMFRLKIESIENALRSHGEKPAMSEENVRLA
                     RAAYSVALEAALERQTPQMRERFEKTMANLDGSTDEDVCDAFGIIYSAILDTVPEDPE
                     GNVLKAFFTGKLL"
     gene            complement(1668..>1924)
                     /locus_tag="ACZPB6_10490"
     CDS             complement(1668..>1924)
                     /locus_tag="ACZPB6_10490"
                     /inference="COORDINATES: protein motif:HMM:NF025695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Abi-alpha family protein"
                     /protein_id="HIR1184927.1"
                     /translation="KELQLYLEHLQTLGLLEISWSGTIANEECYEKVMQSPSVVNFKR
                     SYEEKGKVLTFSKGYLSVTHFGDGFARACRFAAKGAKKDS"
BASE COUNT          460 a          571 c          521 g          372 t
ORIGIN      
        1 aaccggcaaa ctggaactgg cggaaacgaa tccgctggtc tatgtgcacg gtggatatta
       61 tgagctggga gaaaagatag gcaaattcgg atggtcagta tgaccgacag gaaacgggcg
      121 gtcctcaacg tagatgtcgc cgaacacatc aaccctcagg cgtccttcac cgccgggttc
      181 gacctgcccg acgggaatgc tccaatcccc gtcgtagacg tttccgaaga tgtcggtacg
      241 caactcgccc gccgaggcgc cggcggcgat gacgccaaga ctgaaaagca acagaatgga
      301 acgacgcatg gttgatctcc accaaacgaa aaagtgtcgg gcaccccgcc gccgaagccg
      361 ctcaatgagg aggaaacggt ctcgacggca ggatgacctc ctccatcctc atgatgcctc
      421 ttgccgcgtc agcatgagac gcactctggc atgaacgacg cacagacctt cgaccacaag
      481 tcgcctcaag gagacattgc ctttggatct gagataggat tgaagttcac cctggccttt
      541 cccggagctt tcatgcagaa gattcttccc gccgtcctca tgctttgcgc cctcgccgca
      601 ggctcgcagc ccgtctgcgc agccacggac aatcaactca tgcgcgagga gatcacggcc
      661 ggcatgaccg atcccgccgt gatcgaggga agcgccgacg ccttcggctt cagcggagaa
      721 aaggccgagg cttatgccga cttcatccgc gatcttgcgg cgagcaggct ctttctcgac
      781 catgtcgtcg aacagttcaa gaaggcgggc attgacggcg cgaagctgcg cgccgtgcac
      841 gatcacggca cgagcgacga gcttgcggcc ctctccgagg aaatccttca gggaagcctt
      901 ctgctgaccc agcgcctgat ggctgagggc gtgccctatt cgagtccgaa ggtgatggag
      961 gccgagatgc gcacgacgaa ggcactctgc ggccgtctgt cggccaaggc ctgccgagac
     1021 ctcaatctcg tgggcgcgca gggacttgcc tacgacgatc tggatacggc ggccagaacg
     1081 ctcacggccg acatgagcga agaggacgtg aggaagatgt tcaggctcaa gatcgagagc
     1141 atcgagaacg ctctcaggag ccacggcgaa aagcctgcaa tgtcggagga gaacgtcagg
     1201 ctcgccaggg cggcctattc cgtcgccctt gaggccgcac tggaaaggca gacgcctcag
     1261 atgcgcgagc gcttcgagaa gaccatggcg aaccttgacg gctcgaccga cgaagacgtc
     1321 tgcgatgcgt tcgggatcat ctacagtgcc attctcgaca cggtccccga agatcccgag
     1381 ggaaacgtcc tcaaggcctt cttcacgggc aagttgctct gaacgccctt ccccgcaagc
     1441 cagcctgaag gcccgaaaca aaactgccgc ccctttaaac aagaaggtgg cagttgtttt
     1501 tggaacatcg gccccgctcg cgaggggcga agccgatgct gccgacgaac gccggtactc
     1561 aataaccgtt atcttgacga gggcgaggaa agcctgcgat caactggatt ccaataaagc
     1621 tcattaagac aatcctcgaa ttcactgata tcaaatcgat actttcttta cgaatctttt
     1681 ttagctcctt tagcagcgaa cctacatgcc cttgcaaaac catctccgaa atgcgtcacc
     1741 gacaaatacc ctttactaaa ggttaacact ttaccttttt cctcataact tctcttaaag
     1801 ttaaccaccg atggcgattg catgactttt tcatagcatt cttcattggc tatagtgccg
     1861 ctccaagata tttccagaag ccccaatgtc tgcaaatgtt ctagataaag ctgaagctcc
     1921 ttcg
//
LOCUS       DBMDTI010000351         1921 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_12983_length_1921_cov_2.515934, whole genome shotgun sequence.
ACCESSION   DBMDTI010000351 DBMDTI010000000
VERSION     DBMDTI010000351.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12983_length_1921_cov_2.515934"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="ACZPB6_10495"
     CDS             complement(<1..459)
                     /locus_tag="ACZPB6_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1184928.1"
                     /translation="MKLNRRQLIGGAAVMTAMPGIPSAFATVKEETRDLVIVGGGLGG
                     LSAANAAVNKGYKALVIEKLPFLGGAGIMPEGSLGINTKYQKAHGIKTTAQQVLDAAL
                     QFHHFRADPAVLRVLIEESTRTIDEIIDMGVELRGIRTMYPKEESLMCWHL"
     gene            complement(575..826)
                     /locus_tag="ACZPB6_10500"
     CDS             complement(575..826)
                     /locus_tag="ACZPB6_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184929.1"
                     /translation="MVSNVLCDLAAGDLVDLSAPTGGFRLPEGDAPLVFITAGIGAAM
                     LRSVDEHRSVTFKRAGRPLGTARPSFIRPLPRGFPIVTV"
     gene            complement(865..1272)
                     /locus_tag="ACZPB6_10505"
                     /pseudo
     CDS             complement(865..1272)
                     /locus_tag="ACZPB6_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012272.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
     gene            complement(1445..>1921)
                     /locus_tag="ACZPB6_10510"
     CDS             complement(1445..>1921)
                     /locus_tag="ACZPB6_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3400 domain-containing protein"
                     /protein_id="HIR1184930.1"
                     /translation="IFPGCRIIDIHEFLLERGITMPVHADTYLYHDPCHTPLRLNPPL
                     ETVKKLTGANVVQSERCCGESGLFAVKRPDIASQVRHRKFEEIEKARAKAGSKGHVKM
                     LTTCPGCLQGLSRYEERADMDAEYIVVEMARQILGANWRDQFIRTVKNGGIEKVLV"
BASE COUNT          326 a          529 c          709 g          357 t
ORIGIN      
        1 taggtgccag cacatcagcg actcttcctt cggatacatc gtgcggatgc cgcgaagttc
       61 gacgcccatg tcgatgattt catcgatcgt gcgggttgat tcctcgatga ggacgcgaag
      121 cacggcgggg tcggcgcgga agtggtggaa ctggagggcg gcgtcgagga cctgctgggc
      181 ggtcgtcttg atgccatggg ccttctggta cttcgtgttg atgccgagcg agccttcggg
      241 catgatgcct gcgccgccga ggaacggaag cttttcaatg actagcgcct tgtagccctt
      301 gttgacggcc gcgttcgcag cggaaagacc accgaggccg ccgcccacga tcacgaggtc
      361 gcgggtttcc tccttcacgg tggcgaaggc ggacgggatg ccgggcatgg cggtcatgac
      421 ggccgcgccg ccgatgagct gacgacgatt gagtttcata actgttctcc ttgtggacgc
      481 cgtggtctcg gcgtcttgtt cttgcacatg cggttcggtc aaaaaccgca ttgagaacat
      541 gatcggattt tcgggattgg gtttctgtgt gaattcacac agtcacaata gggaaacccc
      601 gaggaagtgg ccgaatgaaa gaagggcggg ccgtcccgag gggacggccc gcccttttga
      661 aggtgacgga gcggtgctcg tccacgctgc ggagcatggc ggcgccgatg cccgccgtga
      721 tgaagacgag cggggcgtcg ccctcgggca ggcggaagcc gcccgtcggc gcggagaggt
      781 cgacgaggtc gccggccgcg aggtcgcaga ggacgttcga gaccatgcct gcagggaggc
      841 cgtctcttgc ggaaatgcgg cggaccaggc gtcgacgacg gagggcgtcg caatctcgcc
      901 gagcacctcg cggatggcgg cgatcagatg acggccgatg atcggatagt gctcggcgcg
      961 aacgcccacc gagcagtgcc gcatggcggt atgcctcagg gcgcccgcaa gcacggacgg
     1021 atcgtcgatg agggtcgcgt atgcgtggac cgcatgtgcg agcgcacgcg gctgggagcc
     1081 cgtgcgctgg tgcgtcatgt tgaagagctg cttgagctcc ggattgtggg tgaggaggcg
     1141 cgagtagaag tgagcgagca gcgcctcgcc gttggtggcg aggatgggag cagttgcctt
     1201 gacaacggcc ttctgttggt cgtcagcatg gtgtgggttc cttctttgtg ttgtttctct
     1261 tagccgaagg ataagtcagg gcgcgcgccc cgagcctgtt ctgaagaata cgcttgcatt
     1321 tcgaaacgca cgcgcaagag ggaaaaccat ggggcggcgt gcttggacgc cggggcgcga
     1381 agccgtcgga cgcggctgcg aactgcgggg cagggaaagg gggcagcccg gagagaaggc
     1441 gttcctagac gagaaccttc tcgatgccgc cgttcttcac ggtgcggatg aactggtcgc
     1501 gccagttggc gcccaggatc tggcgggcca tctcgacgac gatgtactcg gcgtccatgt
     1561 cggcgcgctc ctcatagcgg gagaggccct gcaggcagcc ggggcacgtg gtgagcatct
     1621 tgacgtggcc cttgctgccg gccttggccc tggccttctc gatctcctcg aacttgcggt
     1681 gacgcacctg ggaggcgatg tcggggcgct tgacggcgaa gaggcccgac tcgccacagc
     1741 agcgctcgga ctgcacgacg ttggcgccgg tgagcttctt gaccgtttcg agcggcgggt
     1801 tgagcctgag cggcgtgtgg cacggatcgt ggtagaggta cgtgtcggcg tgcacgggca
     1861 tggtgatgcc gcgctcgagc aggaactcgt ggatgtcgat gatgcggcag cccgggaaga
     1921 t
//
LOCUS       DBMDTI010000352         1901 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_13165_length_1901_cov_2.227222, whole genome shotgun sequence.
ACCESSION   DBMDTI010000352 DBMDTI010000000
VERSION     DBMDTI010000352.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13165_length_1901_cov_2.227222"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(275..1264)
                     /gene="ygfZ"
                     /locus_tag="ACZPB6_10515"
     CDS             complement(275..1264)
                     /gene="ygfZ"
                     /locus_tag="ACZPB6_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR1184931.1"
                     /translation="MNCNANACTTADAGDDAGRFALTTTAPCCRVTEYGLMRVSGKDA
                     VTFLHGQFTNAVTGLADEVRNAGYCTPKGRLLATFRVWMDGDDVMMLVPKAVAPAFFK
                     RLRMYVLRADVTFTCTGGDVVMTGVPAGAEAFLEAAGLPVPAPSRVVRHDGLVIVDAE
                     PASKVEGFCAGGRRALIIAPKDDARFADASTDSALWWASEIAAGKTTVWPGSRELFVP
                     QAVNYELSGGVVFNKGCYPGQEVVSRVQHIGETSRRAAFGLVAGTEAPLPGAPVYCDG
                     AEAGFVTQAVAGEISTLVAFSSLRSALTGRLTLTPEGAPISILPLPYKIPSAA"
     gene            complement(1261..>1901)
                     /gene="mltG"
                     /locus_tag="ACZPB6_10520"
     CDS             complement(1261..>1901)
                     /gene="mltG"
                     /locus_tag="ACZPB6_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR1184932.1"
                     /translation="EAELRRALGIDAGSLEGWFAPDTYHYTSGSADLAVMKQAVAKQK
                     ALLAKAWESRSEAARVKTPYEALTLASIIEKETGLATDRHLVSSVFNNRLSIGMPLQT
                     DPTVIYGLGEKFSGNITRKDLQTPTPYNTYVIPALPPTPIAAPTWASIEAAVNPADTR
                     FLYFVSKGDGSSHFSQSLAEHNRAVRQFILNRPKTQKKAKPKELQPTKETAR"
BASE COUNT          315 a          545 c          714 g          327 t
ORIGIN      
        1 aaatttggaa aaacgctcaa aaccatgttt tccctcaggc gcaggtgccg gaatgttgcg
       61 cggacgcatc caaaggaagc cctctcacgg gacggcacgg cggccctgca gggagccgga
      121 agatggctcg gttgaatttg ccattttgcc ttccgtataa gccgacataa catgttctgt
      181 ctgttgcgca ggagaaagtg tgaggctcat gaaggagaag gcggccgtca ccacagaggg
      241 atgacggccg ccggtcgaat gcggatcagt gcgatcaggc tgcggacggg atcttgtagg
      301 gcagcggcag gatcgagatc ggcgcgcctt cgggcgtgag cgtgaggcgg cccgtgaggg
      361 cggagcgcag ggacgagaag gcgacgaggg tcgagatttc gcccgcgacg gcctgcgtca
      421 cgaagccggc ttcggcgccg tcgcagtata cgggagcgcc ggggagcggg gcctcggtgc
      481 cggccacgag gccgaaggcg gcgcggcggc tcgtctcgcc gatgtgctgg acgcgggaga
      541 cgacttcctg tccggggtag cagcccttgt tgaagacgac gccgcccgaa agttcgtagt
      601 tgacggcctg cggcacgaag agttcgcgtg agcccggcca gacggtggtc ttgcctgcgg
      661 cgatttcgga ggcccaccag agggcggagt ccgtcgaggc gtcggcaaag cgggcgtcgt
      721 ccttcggggc aatgatgagc gcacggcggc ctcctgcgca gaagccctcg actttggagg
      781 caggttcggc gtcgacgatg acgaggccgt cgtggcgaac gacgcggctg ggcgccggga
      841 cgggcaggcc cgcagcttcg aggaaggcct cggcgccggc gggaacgccc gtcatgacga
      901 cgtcgccgcc cgtgcacgtg aaggtcacgt cggcgcggag cacgtacatt cgcaggcgct
      961 tgaagaaggc gggcgcaaca gccttcggga cgagcatcat gacgtcgtcg ccgtccatcc
     1021 agacgcggaa cgtggcgaga aggcggccct tcggggtgca gtagcccgca ttgcggactt
     1081 cgtcggcgag gcccgtgacg gcgttcgtaa actggccgtg caggaacgtg acggcgtcct
     1141 tgccagagac gcgcatcaga ccgtattcgg tgacgcggca gcagggggcg gtcgtcgtga
     1201 gggcaaatcg gcctgcgtcg tcgccggcgt cggcggtcgt gcaggcattg gcgttgcagt
     1261 tcatcgtgct gtttccttcg taggttgcag ttccttgggc ttggccttct tctgcgtctt
     1321 cgggcggttc aggatgaact gcctgacggc gcggttgtgt tcggcgaggc tctgcgagaa
     1381 atggctcgag ccgtcgccct tcgagacgaa gtagaggaac ctggtgtcgg cgggattgac
     1441 cgcagcctcg atcgacgccc aggtgggcgc ggcgatcggc gtgggcggaa gcgccggaat
     1501 gacgtacgta ttgtaagggg tcggcgtctg cagatccttg cgggtgatgt tgccgctgaa
     1561 cttctcgccg aggccgtaga tgacggtcgg gtcggtctgc agcggcatgc cgatgctcag
     1621 gcggttgttg aagaccgagc tcacgaggtg gcgatcagtg gcaaggcccg tctccttctc
     1681 gatgatggag gcgagcgtga gcgcctcata gggcgtcttc accctggcgg cctcggagcg
     1741 ggactcccag gccttggcca gaagggcctt ctgcttggcg acggcctgct tcatgacggc
     1801 aaggtcggcg ctccccgacg tgtagtggta ggtgtcgggc gcaaaccatc cctcgagcga
     1861 gcccgcgtcg atgccgagcg cacggcgcag ctcggcctcg g
//
LOCUS       DBMDTI010000353         1856 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_13586_length_1856_cov_2.811966, whole genome shotgun sequence.
ACCESSION   DBMDTI010000353 DBMDTI010000000
VERSION     DBMDTI010000353.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13586_length_1856_cov_2.811966"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /gene="mutS"
                     /locus_tag="ACZPB6_10525"
     CDS             complement(<1..1619)
                     /gene="mutS"
                     /locus_tag="ACZPB6_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR1184933.1"
                     /translation="MTETFIHDEDLDLNDFTPAMRQFIEVKRRYPETLVLFRMGDFYE
                     TFFDDAAKANRLIGITLTKRGKLKNGDPIPMAGIPMVSLDQYVARLVRLGESVVIAEQ
                     LGTPGKGLMERRISRIITPGTLTDTSLLSEKDDAVLLSVSRPAGKNAVWGFAWLTLSN
                     GDFFATEVTDEQFDSEVARISPSEVLVSEKLRDAMRETHPELTVTPMPAWHFDAEHGE
                     EALKKTFGLDNLDAWGVTGKTGVLAAANALLDYTAETQVDMIPFISPLKLTDESEYII
                     IDPASRRNLEISDTIRSEGRGPTLFSVLDRCGTSMGSRELRRWLNLPLTKADEARGRH
                     DALEALAENQDFSSFVETRLQGLPDIERIASRIALGTVRPKELAALRDALPLVGELNA
                     EIASREETWFGMTAKTLSLPAEIFSNLEAALLPEPATLLREGDVIRSDFNEELGLLRQ
                     MRDNTGQFLLDLEKREREKTGLTSLRVEYNRVHGFYIEVSKGQAASVPVEYHRRQTLK
                     NTERFITPELKNYEDQALSSKERSAALEKEL"
BASE COUNT          311 a          529 c          661 g          355 t
ORIGIN      
        1 tagagctcct tctcaagcgc ggcggagcgc tccttcgagg agagtgcctg atcctcgtag
       61 ttcttgagct cgggcgtgat gaagcgctcg gtgttcttga gcgtctgtcg gcggtggtat
      121 tcgacgggga cggacgcggc ctgtcccttg gagacctcga tgtagaagcc gtggacgcgg
      181 ttgtactcga cgcgaaggct cgtgagcccc gtcttttcgc gctcgcgctt ttcaaggtcg
      241 aggaggaact ggcccgtgtt gtcgcgcatc tggcgcagga gtcctagctc ctcgttgaaa
      301 tcggagcgga tgacgtcgcc ctcgcgcaga agcgtggcgg gctcgggaag gagcgcggcc
      361 tcgagattcg agaagatttc ggcgggcagg gaaagcgtct tcgccgtcat gccgaaccag
      421 gtctcctcgc gggaggcgat ttcggcattg agctcgccca cgagcggaag cgcgtcgcga
      481 agcgccgcca gttccttcgg acgcaccgtt ccaagtgcga tgcggcttgc gatgcgctcg
      541 atgtcgggta gcccctgaag cctcgtctcg acgaagctcg agaagtcctg attctcggcg
      601 agcgcctcga gggcgtcgtg gcggccgcgg gcctcgtcgg cctttgtaag cggcaggttg
      661 agccagcggc gaagctcgcg gctgcccatc gacgtgccgc agcggtcgag cacggagaag
      721 agcgtggggc cccggccttc gcttcggatc gtgtccgaga tctcaaggtt gcgtcgactt
      781 gcggggtcga tgatgatgta ttcggattcg tccgtgagct tcagcgggct gatgaacgga
      841 atcatgtcga cctgagtctc ggccgtatag tcgagaagcg cgttggcggc tgcgagcacg
      901 cccgtcttgc ctgtcacgcc ccaggcgtca aggttgtcga ggccgaacgt cttcttgaga
      961 gcttcttcgc cgtgctcggc atcgaagtgc caggcgggca tcggcgtcac ggtgagctcg
     1021 gggtgcgtct ccctcatggc atcgcggagc ttttcggaga cgaggacctc ggagggcgaa
     1081 atgcgcgcca cttcggagtc aaactgctcg tccgtcacct cggtcgcgaa gaagtcgccg
     1141 ttcgagagcg tgagccaggc aaagccccag acggcgttct tgcctgcggg tcgggagacg
     1201 gagagaagca ccgcgtcgtc cttttcagaa agaagcgagg tgtccgtaag ggtgcccggc
     1261 gtgatgatgc gagagatgcg gcgctccatc aggcccttgc ccggcgtgcc gagctgctcg
     1321 gcgatcacga cggactcgcc gagtctcacg aggcgggcga cgtactggtc gagcgagacc
     1381 atcggaatgc cggccatcgg aatcggatcg ccgttcttga gctttccgcg cttcgtgagc
     1441 gtgatgccga tcaggcggtt ggccttggcg gcgtcgtcga agaacgtctc gtagaagtca
     1501 cccatgcgaa acaggacgag cgtctcgggg taccggcgct tcacctcgat gaactgacgc
     1561 atcgcaggcg tgaagtcgtt gaggtcaagg tcttcgtcgt gaatgaaagt ctcggtcatg
     1621 ggatcttcag ggaagggaga gcgcggatgc gggatccggt gatgaaaaag ggccggacga
     1681 cgaatcggtc cggcccgtca ggctgctggt gcagtcgagg tattaggcct tctgggcttc
     1741 gccggcggcg ggcaacttgc ccacgagcgg caggagctgg gcgaagatgc gcggcgtgcc
     1801 ggcgcagacg cggccctgtt caagccagtc ttcgccgccc gaaaggtcgg tgatga
//
LOCUS       DBMDTI010000354         1850 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_13638_length_1850_cov_2.258433, whole genome shotgun sequence.
ACCESSION   DBMDTI010000354 DBMDTI010000000
VERSION     DBMDTI010000354.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13638_length_1850_cov_2.258433"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(301..1014)
                     /locus_tag="ACZPB6_10530"
     CDS             complement(301..1014)
                     /locus_tag="ACZPB6_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HIR1184934.1"
                     /translation="MAAFEITGGIDPLLAVTLEKGEAILAESNAMVAMDTALSLKGKT
                     RGGLMGSIARKFVNDETFFQQRIEADDQAGTVLLAPNLPGDVRILDVGPAQYLLSDGA
                     FLACTEGVELSTKSQGIGRALLGDSGGFFVMGTEGSGRIAVSGFGSIRELDVTPERPI
                     TVDNGHLVAWDARLSYDLSINTGKSGFLGKLVNSQTTGEGVVLKFRGTGKVLVCSRNK
                     GGFLDWILGNMPAQKTVEN"
     gene            complement(1245..>1850)
                     /locus_tag="ACZPB6_10535"
     CDS             complement(1245..>1850)
                     /locus_tag="ACZPB6_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1184935.1"
                     /translation="RCAGCFACAVACKEENQVAPGIFWEKIKRDENVRDNVINWFRMS
                     CMHCDDPACMKVCPMKAIHKGPHGEVLVDQQKCIGCKMCANACPYDVPVFNTEGRTSY
                     FGDKQPLVVRPAKPHTERTPGKAEHCTLCVHRTERGLLPACVEACGIHAMTLVDYDNP
                     TPETAKLIEAARPMNEAAGTHPKIRYIARHMDAAKMAVKLG"
BASE COUNT          314 a          553 c          619 g          364 t
ORIGIN      
        1 gggctggaaa gacacacgcc tttcatcggg gacaacaaat agattggaag agagaggttt
       61 gccgcgttcc tccatccgaa tgaattcgga ggcttcctgc ggctgtttct gtgaaaaacg
      121 ccgccatttt gcgcattcgc ttctcattcc ctgcaggaaa ggcgtattca ggcaaagacg
      181 caacgtgccg gaggcggatt tcggtgcagg gaaaaatcct gagagccgaa gacgcagaaa
      241 gggcgcccgt ccgaatggtc agggcgccct ttcgcgttca tgtcgggtgc cttggctgcg
      301 tcagttttcg acggtcttct gcgcgggcat gttgccgagg atccagtcga ggaagccgcc
      361 cttgttgcgc gagcagacga ggaccttgcc cgtgccgcgg aacttgagca cgacgccttc
      421 gcccgtcgtc tggctgttga cgagcttgcc gaggaagccg ctcttgcccg tgttgatgct
      481 caggtcgtag ctcaggcgtg cgtcccaggc gacgaggtgg ccgttgtcga ccgtgatcgg
      541 gcgctcgggc gtgacgtcga gctcgcggat ggagccgaag cccgagacgg cgatgcggcc
      601 gctgccctcg gtgcccatca cgaagaagcc gccggagtcg ccgagcagcg cgcggccgat
      661 tccctgggac ttcgtcgaga gttcgacgcc ctcggtgcag gcgagaaagg cgccgtccga
      721 gagcaggtac tgcgcgggac ccacgtcgag gatgcgcacg tcgccgggaa ggttcggcgc
      781 gaggagcacc gtgcccgcct ggtcgtccgc ctcgatcctc tgctggaaga acgtctcgtc
      841 gttcacgaac ttgcgggcga tcgagcccat gaggccgccg cgggtctttc ccttgaggga
      901 gagcgccgtg tccatggcga ccatggcgtt cgactcggcg agaatcgcct cgcccttctc
      961 aagcgtcacc gcaaggagcg gatcgattcc acccgtaatc tcaaaagcag ccatctcgtg
     1021 gtctccggaa gtgattcgat gatcatgcaa ttatagaaag ccggccttcg ccgccatccc
     1081 ctgcgtcaca ttccgaaaag gctttttctt tttttccgcc ttcgggaggg cgcggcggga
     1141 gaacgaatcg aaggacaagc cgaaggacga tgggcgggaa aaaggaaggg ccgcgtcctt
     1201 cccatgaagg ccgcggcccc ttaggaccgg tagtcagccg gcggctagcc gagcttcacc
     1261 gccatcttcg cggcatccat gtgccttgcg atgtagcgga tcttcggatg cgtgcccgca
     1321 gcttcgttca tcgggcgggc ggcctcgatg agttttgctg tttcgggcgt cgggttgtcg
     1381 tagtcgacga gcgtcatggc gtggatgccg caggcctcga cgcaggccgg tagcaggccg
     1441 cgctcggtgc ggtgcacgca cagcgtgcag tgctcggcct tgcccggcgt gcgctcggtg
     1501 tgaggcttgg ccggacgcac gacgagcggc tgcttgtcgc cgaagtagct ggtcctgcct
     1561 tcggtgttga agacgggaac gtcgtacggg caggcattgg cgcacatctt gcagccgatg
     1621 cacttctgct ggtcgacgag cacttcgccg tgcgggccct tgtggatcgc cttcatcggg
     1681 cagaccttca tgcaggccgg atcgtcgcag tgcatgcagc tcatgcggaa ccagttgatg
     1741 acgttgtcgc ggacgttttc gtcgcgcttg atcttttccc agaagatgcc gggagcgacc
     1801 tggttttctt ccttgcaggc gaccgcgcag gcaaagcacc cggcgcagcg
//
LOCUS       DBMDTI010000355         1838 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_13751_length_1838_cov_2.557858, whole genome shotgun sequence.
ACCESSION   DBMDTI010000355 DBMDTI010000000
VERSION     DBMDTI010000355.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13751_length_1838_cov_2.557858"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="ACZPB6_10540"
     CDS             <1..788
                     /locus_tag="ACZPB6_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184936.1"
                     /translation="DVVILADLPFLRLRQILPAMMLSKEYSFSEIVRNSQQAIEYMPR
                     VRLTHDGSRWRLYADGVPVHLEPKLLGLYAWLLLRTKLGRETPVSYGMLPSEVFLHLR
                     LQFVRVLGLILTETRRGTACRSHLGVEEHVLEQAVRSLQTEGIESDAEFYRAFKAATG
                     AGGDARSAEVHKAFVNAERSFSKNVSELRSKCNDKIRAELADQIPDVTGRRFNSYLVE
                     SNGQKDATAYGLQISPENIELPQILLDLCKPVETGAVHRWREV"
     gene            906..1316
                     /locus_tag="ACZPB6_10545"
     CDS             906..1316
                     /locus_tag="ACZPB6_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184937.1"
                     /translation="MTDSVTLRPDPQAASRIEFTALRDDGESTLSIVVDADGGWRLSE
                     TDECARSGTISTGCLHDLIVGFLSAFGIEDEMTLELTEIRGSWFVRFLMAGVPVSSKS
                     GPILSEAVSPYRTLDGLLRQCFGTPRGIFSDRTT"
     gene            1333..>1838
                     /locus_tag="ACZPB6_10550"
     CDS             1333..>1838
                     /locus_tag="ACZPB6_10550"
                     /inference="COORDINATES: protein motif:HMM:NF037501.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Swt1 family HEPN domain-containing protein"
                     /protein_id="HIR1184938.1"
                     /translation="MSQSESPRDFYHRLENGLSPLIRLMLAEHLPKIGGRKWWEACVL
                     SSLTPIQLEYVREPENLEQFDFPALVNIVHHNWRTLRPSLGIGNEFTNYLFSLKTLRN
                     EVEHRPDLVLDDERRRHLEQSARLAMRLLNKDETTGPNLCTPLVARSMRAGILASAIA
                     VVGLGVLLL"
BASE COUNT          334 a          665 c          517 g          322 t
ORIGIN      
        1 cggacgtcgt gatcctcgcc gacctcccct tcctgcgcct tcgccagatt cttcccgcca
       61 tgatgctctc gaaggaatac tccttcagcg agatcgtccg gaactcccag caggcgatcg
      121 aatacatgcc gcgcgtgcgc ctcacgcacg acggcagccg ctggcgcctc tacgccgacg
      181 gcgtgcccgt gcacctcgag cccaagctcc tcggcctcta cgcctggctg cttcttcgca
      241 cgaagctcgg ccgggagacg cccgtctcct acggcatgct cccgtcggag gtcttcctgc
      301 acctgcggct tcagttcgtc cgggtgctcg gcctcatcct cacggaaacc cgtcgcggca
      361 cggcctgcag aagccacctc ggcgtcgagg agcacgtgct cgagcaggcc gtcaggtccc
      421 ttcagaccga gggcatcgag tcggacgccg agttctaccg agccttcaag gctgcaacgg
      481 gcgccggagg cgacgcacga agcgccgagg tgcacaaggc cttcgtgaac gccgagcgca
      541 gcttctcgaa gaacgtctct gagcttcgct cgaagtgcaa cgacaagatc cgcgccgaac
      601 tcgcggacca gattcccgac gtgacgggcc gccgcttcaa cagctacctc gtcgaatcga
      661 acggccagaa ggacgcaacc gcctacggcc tgcagatctc gcccgaaaac atcgagctgc
      721 cgcagatcct cctcgacctg tgcaaacccg tcgagacggg cgccgtgcac cgctggcgcg
      781 aagtctgagg acgaattccg caaatctgcg gaattccccg ccttcccgcc ccgcccgcac
      841 aatcggatca tggcggcgaa tccgcgccgc atcttgaagg gcgacttcgc ccgtggacaa
      901 acgtcatgac cgattccgtc acgctgcggc ccgacccgca ggccgcctcc cgcatcgaat
      961 tcaccgctct cagggatgac ggcgaaagca cgctctccat cgtcgtcgac gccgacggcg
     1021 gatggcgcct cagcgaaacg gacgaatgtg ctcgttcggg aacgatttca accggctgcc
     1081 tgcacgatct gattgtgggt ttcctcagtg ctttcggcat tgaggatgaa atgaccttag
     1141 aattgacgga gatccgcgga tcctggttcg ttcgctttct catggcgggc gtgcccgttt
     1201 cctcgaagtc cggtccgatc ctttccgaag cggtctcgcc ataccggacg ctcgacggcc
     1261 tgctgagaca gtgcttcggc acgccgcgcg ggatcttctc cgaccgaacc acctgacccg
     1321 cgaccaccgt ccatgtccca aagcgaatcg ccccgcgact tctaccaccg cctcgaaaac
     1381 gggctttcgc cgctcatcag gctcatgctc gccgagcatc tgccgaaaat cggcggccga
     1441 aaatggtggg aggcctgcgt gctgagttct ctcacgccca tccagcttga gtacgtccgc
     1501 gagccggaga acctcgagca gttcgacttt cccgctctcg tgaacatcgt gcaccacaac
     1561 tggcgcacgc tcaggccctc gctcggcatc ggcaacgagt tcacgaacta cctcttctcg
     1621 ctcaagacgc tgcgcaacga agtcgagcac aggccggacc tcgtcctaga cgacgagcgc
     1681 cgcaggcatc tcgagcagtc ggcccgtctg gccatgagac ttctcaacaa ggacgagacc
     1741 acgggcccga acctctgcac gccgctcgtc gcccgcagca tgcgcgcggg cattctcgca
     1801 tccgccatcg ccgtggtcgg actcggcgtc ctccttct
//
LOCUS       DBMDTI010000356         1827 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_13868_length_1827_cov_1.720162, whole genome shotgun sequence.
ACCESSION   DBMDTI010000356 DBMDTI010000000
VERSION     DBMDTI010000356.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13868_length_1827_cov_1.720162"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            207..944
                     /locus_tag="ACZPB6_10555"
     CDS             207..944
                     /locus_tag="ACZPB6_10555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1184939.1"
                     /translation="MTLSDDVRDYWNTRAEGYSLHNCDELDGGTGLVLEHLFRTELGA
                     KPGMRALDAGCGPGIFSIVLARLGCRVTGMDLSPEMLEHAGANAKSRGLEADFRLGDV
                     TDPGMEPSSLDLIVSRCVLWNLPAPAEAYRRWMSLLKPGGRLLVIDGNHYLHLFDERY
                     ALWALRSAPAPGHAARYMRGVSSERIDRIARDLPLSCIERPAWDVEVLESLGAEVRVA
                     SEDRRTVATGGGEAQVTTSFCIVARKP"
     gene            1048..>1827
                     /locus_tag="ACZPB6_10560"
     CDS             1048..>1827
                     /locus_tag="ACZPB6_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter-like transporter"
                     /protein_id="HIR1184940.1"
                     /translation="MGWLQALLTDSGSFTHALLAYSLIIAAGLWIGRFRFFGVSVGVT
                     CVLFLGLFARYLGVDVDPSIISFFRNFGLVLFVFFIGLQVGPSFFSTLRNSGWGLNGL
                     MCLSVGVSLLLTVGIWAASDGALALPQLLGVHFGAVTSTPGLGATQEALAAMGYKGDI
                     TVGYACAYPVSIIGVILTLLFVRKVFGIDVKHEEMLWEEAQKKRAQTPIYFHVTLSNH
                     ALDNHTLREIRSLVGRNFICSRILHDGVITSPTADTVVHEGD"
BASE COUNT          291 a          536 c          637 g          363 t
ORIGIN      
        1 ggacgttttt caggcaacgc tttcgatcgg gcggagtcgc tagcccgatc gtcgtagtac
       61 cgccggccag gagcggtgtg gcggagtttg ggagtatcat acgaaatcca aactcttcat
      121 acgcctgatg ccacccgacg atgcacgggg aggcttgagc gtgaaggcga tacgcacgac
      181 aacagaacga agaaaggaga ttcgccgtga cactctcgga cgacgtgcgc gactactgga
      241 acacacgcgc ggaaggctat tcgcttcaca actgcgatga gcttgacgga gggacggggc
      301 ttgtgcttga gcacctgttc aggacggagc tcggggcgaa gcccgggatg cgggcgcttg
      361 atgcgggctg cggtccgggg atcttcagca tcgtgttggc gagactcggc tgcagggtga
      421 cgggcatgga cctgtcgccc gagatgctcg agcacgcggg ggcgaacgcg aagagccgcg
      481 ggcttgaggc ggacttcagg ctgggcgacg tgacggatcc cgggatggag ccgtcgtcgc
      541 tggacctgat cgtctcgcgg tgcgtgctct ggaacctgcc tgcgcccgcc gaggcgtaca
      601 ggcgctggat gagtcttctg aagccgggcg gcaggcttct tgtgatcgac ggcaatcatt
      661 atctgcatct ctttgacgag cgctatgcgc tctgggcgct gaggtccgcg cccgcgccgg
      721 ggcatgcggc gaggtacatg aggggcgtga gcagcgagcg catcgacagg attgcgcgcg
      781 atctgccgct gtcgtgcatc gaacgtcccg cgtgggacgt cgaggtgctc gagtcgctcg
      841 gcgccgaggt gcgcgttgcg agcgaggacc gcaggacggt ggcgacgggc ggcggagagg
      901 cgcaggtgac gacgagcttc tgcatcgtgg cccggaagcc ctaagcgcag gagcgcaggg
      961 gccgcccgtg tggcggctcg ggggcgccct gaactacaat gacggattgc aatttgttgc
     1021 ggattcaaac ttccggaaat ttctgtcatg ggctggcttc aagctcttct taccgactcg
     1081 ggctccttca cgcacgcgct tctcgcgtat tcgctcatca ttgcggcggg tctctggatc
     1141 gggcgcttca ggttcttcgg cgtctcggtc ggcgtcacct gcgtgctttt tctcggcctt
     1201 tttgcgcgct atctgggcgt tgacgtcgac ccctcgatca tttcgttctt ccgcaacttc
     1261 ggcctggtcc tcttcgtctt cttcatcggc ctgcaggtgg ggccgtcgtt cttctcgacc
     1321 ctgagaaaca gcggctgggg gctcaacggg ctgatgtgcc tgtcggtggg cgtgagcctg
     1381 cttctgacgg tcgggatatg ggcggcttcg gacggcgcgc tggcgctgcc gcagctcctc
     1441 ggcgtgcact tcggcgccgt gacgagcacg cccggtctgg gcgcgacgca ggaggcgctc
     1501 gcggccatgg ggtacaaggg cgacatcacc gtgggttatg cctgcgccta tcccgtgtcg
     1561 atcatcggcg tcatcctgac gcttctcttc gtgaggaagg tcttcgggat cgacgtcaag
     1621 cacgaggaga tgctctggga ggaggcgcag aagaagcgcg cccagacgcc gatctacttt
     1681 cacgtgacgc tctcgaacca tgcgctcgac aatcatacgc tgcgcgagat ccgctctctc
     1741 gtgggccgca acttcatctg ctcgcgcatt ctgcacgacg gcgtgatcac gagtccgacg
     1801 gccgacacgg tcgtgcacga gggcgac
//
LOCUS       DBMDTI010000357         1815 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_13988_length_1815_cov_2.396149, whole genome shotgun sequence.
ACCESSION   DBMDTI010000357 DBMDTI010000000
VERSION     DBMDTI010000357.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13988_length_1815_cov_2.396149"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="ACZPB6_10565"
     CDS             <1..424
                     /locus_tag="ACZPB6_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1184941.1"
                     /translation="LWEHALKTGVVKKFDTLKDLAAGYGIDYATLEKSNETFNGYMKA
                     MKDPEFDCMFFKGASVNDKGPFYGVRLWPRVHHCMGGLEINEHAQVMSVRGTPIKGLY
                     AAGEATGGVHGMVRLGTVAVADCMIFGRIAARDAFKKA"
     gene            693..>1815
                     /locus_tag="ACZPB6_10570"
     CDS             693..>1815
                     /locus_tag="ACZPB6_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR1184942.1"
                     /translation="MMKKSLLALSVAAAAMGAQAADVQVYGLIDTSLSFVSSDADIAG
                     HDRVNSFSMENAKEFGSRFGLKGSEDLGNGYKVAFVLESGIKSDDGSLDQGGKLFGRE
                     AHVDLVGPFGKLSAGLMPVFGSTLGADGLFRAIDPLFANYTKAFGSGHVTASSWTRVD
                     NALSYVTPTVAGFTGYAMYSFKNDSSKGGVEGHGGESDRYASLAARYKAGALEAVLVA
                     DTTMYGTYRTDTAASQDDGWTVTFGGNYTFDGGVKVLAFTQFFGDQELPAFRGGVGTD
                     GIMAVTGDKGYGFVDGYGVSLGVNVPAMGGVAKAQVAYRDMDNKDNVDFKRVIAALGY
                     DYALSKRTAVYAMTGWSQEKVENGTTEAKPNGYEFTTGIV"
BASE COUNT          338 a          633 c          533 g          311 t
ORIGIN      
        1 gctctgggag cacgccctca agaccggcgt cgtcaagaag ttcgacacgc tcaaggacct
       61 cgctgccggc tacggcatcg actacgcgac gctcgagaag agcaatgaga ccttcaacgg
      121 ctacatgaag gccatgaagg accccgagtt cgactgcatg ttcttcaagg gcgctagcgt
      181 caacgacaag ggtcccttct acggcgttcg cctctggccg cgcgtccacc actgcatggg
      241 cggccttgag atcaacgagc atgcccaggt catgtccgtg cgcggcaccc cgatcaaggg
      301 cctctatgcc gccggcgaag ccacgggcgg cgtccacggc atggttcgtc tcggtaccgt
      361 cgccgttgcc gactgcatga tcttcggccg catcgcagcc cgcgatgcct tcaagaaggc
      421 ctgatcccgg tcaacgcctg aagatgcgcc ctgcgagcgg gaagcctgag acctcccgcg
      481 tgcaggtatc ggcgggcgct tcgtccggtc ggacgcggcg cccgttcccg cccgaaatcg
      541 gcggaaggaa tgccggaggt aaggcggtga ttaaagacag aaacaaaagt actcgttgat
      601 actgtattat tccgtcctgc ccgcaggaga ggcgaagcgc cctcctgact caaagcgcat
      661 tccgcttttc aacccattag gaaatgaaca tcatgatgaa gaagtctctt ctcgcccttt
      721 ccgtggccgc tgcggccatg ggcgcccagg ccgctgacgt ccaggtctac ggcctcatcg
      781 acaccagcct ctccttcgtg tcgagcgacg ccgacattgc cggccacgac cgtgtgaact
      841 ccttctcgat ggagaacgca aaggaattcg gctcccgctt cggtctcaag ggttctgaag
      901 acctcggcaa cggttacaag gtggccttcg tgctcgagtc cggcatcaag tccgacgacg
      961 gcagtctcga ccagggcggc aagctcttcg gtcgcgaagc ccacgtcgac ctcgtcggtc
     1021 cgttcggcaa gctctccgcc ggcctgatgc ccgtctttgg cagcacgctc ggcgccgacg
     1081 gcctcttccg cgccatcgac ccgctctttg cgaactacac gaaggccttc ggctccggcc
     1141 acgtgaccgc ctcctcctgg acgcgcgtcg acaatgctct cagctacgtg accccgaccg
     1201 ttgcgggctt cacgggctac gccatgtact ccttcaagaa cgactcctcc aagggcggcg
     1261 tcgaaggcca cggcggcgaa tccgaccgct acgcctctct cgccgcacgc tacaaggccg
     1321 gggctcttga ggccgtgctc gtcgccgaca ccacgatgta cggcacgtac cgcaccgaca
     1381 ccgccgccag ccaggatgac ggctggactg tcaccttcgg cggcaactac acgtttgacg
     1441 gcggcgtcaa ggtcctcgcc ttcacgcagt tcttcggcga ccaggagctt cccgccttcc
     1501 gcggcggcgt cggcaccgac ggcatcatgg ccgtaaccgg cgacaagggc tacggctttg
     1561 tcgacggcta cggcgtgagc ctcggcgtga acgtgcccgc catgggcggc gtcgccaagg
     1621 cccaggtcgc ctaccgcgac atggacaaca aggacaacgt cgacttcaag cgcgtgatcg
     1681 ccgccctcgg ctacgactac gccctctcca agcgcaccgc cgtctacgcc atgaccggct
     1741 ggtctcagga gaaggtcgag aacggcacga ccgaagccaa gcccaacggc tacgagttca
     1801 cgaccggcat cgtcc
//
LOCUS       DBMDTI010000358         1813 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_14004_length_1813_cov_2.251752, whole genome shotgun sequence.
ACCESSION   DBMDTI010000358 DBMDTI010000000
VERSION     DBMDTI010000358.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14004_length_1813_cov_2.251752"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            649..>1813
                     /locus_tag="ACZPB6_10575"
     CDS             649..>1813
                     /locus_tag="ACZPB6_10575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1184943.1"
                     /translation="MTQIDTSRRGFLKSAVAASAAMMSAAAVHAGIPAKSVAKYDETF
                     DVVIIGSGFAGMACALKAGRAGLKVLMIEKMSTVGGNSAICGGNVACPVNPVQKAQGI
                     KDSKELFIADCLKDGLGINHTNLLSTIADRCNDTIQMVVDCGCEFVPNKMLFEGGHSV
                     PRSYEIKAGTGSGYIRPMHEELKKLKNVVIRTRCKFDDFVMEEDGSVAGIVCRTGYRF
                     NNKAVSDDLENKTGKKSVVRATKGVMLAAGGFSRDIFFRQIQDPRVVPTTDSTNQPGA
                     TAGVLIKALGIGAAPVQLCWLQFLPYCNPREKGFGVSVNFTNHACMDLGLVVDRKTGR
                     RFMDEHAGRKIKSDALFKVIGTDENYPIAVCDDSIVKAINPSFVKLPLEMGTVK"
BASE COUNT          354 a          578 c          546 g          335 t
ORIGIN      
        1 ctcgcaggag aggcccatgc cgacatggcg gtcggcaccg aacttctggt cggcagcctg
       61 agcggtgccg aaggcgaacg tcgcggcgca gagcgaggcg aggatcagcg tcgtcatctt
      121 catttttgtt ttcctttctg ggttctgaga ccggaagcgc ggagtctgga gctccggacg
      181 ggctgtccgt ccgatgcgct ttcgttgggt caaatcatat gcaggcgcgc cggcgtttcc
      241 tacctaccaa agtatgtatt tcatgcatac ccgggtatgg ggttttcggt tggtcaataa
      301 gaaacgtctg aaggagagtg attgttatcg gtgcggaggg actgcggcgg atgttcgccg
      361 gacttttgct gcggggcgga tgcgggggag cggagggggc ggtcccctgg gggaaaccct
      421 ccttcaacgt gtcgtccaca ggaggctttc cgccggagag ggcgtgtttt caaggggttt
      481 gggaaaggga agggtaaacc ctgcatactt tggtatgtgc aaggtccata ccgcggtatg
      541 tagccgaaag gagggcggag gagaagaata gcggtgtcgc cggcgaggag cgagcgagag
      601 aatccgcccc gccggttgtg ttcccaatcc gaatggagaa tcctcagaat gactcagatc
      661 gataccagcc gccgcggctt cctcaagtcc gccgtcgccg cctccgccgc catgatgtcc
      721 gctgccgccg tgcacgccgg cattcccgcc aagtccgttg ccaagtacga cgaaacgttc
      781 gacgtcgtga tcatcggctc cggctttgcc ggcatggcct gcgccctcaa ggccggccgc
      841 gccggtctca aagtcctcat gattgagaag atgtccacgg tcggcggcaa ctcagccatc
      901 tgcggcggca acgtcgcctg cccggtcaac ccggtccaga aggcccaggg catcaaggac
      961 agcaaggagc tcttcatcgc cgactgcctg aaggacggtc tcggcatcaa ccacacgaac
     1021 ctcctctcca ccatcgccga ccgctgcaac gacacgatcc agatggtcgt cgactgcggc
     1081 tgcgaattcg tgcccaacaa gatgctcttc gaaggcggcc actcggttcc gcgttcctat
     1141 gaaatcaagg ccggcaccgg ctccggctac atccgtccga tgcacgaaga gctcaagaag
     1201 ctcaagaacg tcgtgatccg aacccgctgc aagttcgacg acttcgtcat ggaagaagac
     1261 ggctccgtcg ccggcatcgt ctgccgcacg ggctaccgct tcaacaacaa ggccgtctcc
     1321 gacgacctcg aaaacaagac cggcaagaag tccgtcgtcc gcgccacgaa gggcgtcatg
     1381 ctcgccgccg gtggcttctc ccgcgacatc ttcttccgtc agatccagga cccgcgcgtc
     1441 gtcccgacga ccgactccac gaaccagccc ggcgccacgg ccggcgtgct gatcaaggcc
     1501 ctcggcattg gcgccgctcc ggttcagctc tgttggctcc agttcctgcc gtactgcaac
     1561 ccgcgtgaaa agggcttcgg cgtttccgtg aacttcacga accacgcctg catggacctc
     1621 ggcctcgtcg tcgaccgcaa gaccggccgc cgcttcatgg acgaacacgc cggccgcaag
     1681 atcaagtccg acgccctctt caaggtcatc ggcacggacg aaaactaccc gatcgccgtc
     1741 tgcgacgact ctatcgtgaa ggccatcaac ccgtcctttg tcaagctccc gcttgaaatg
     1801 ggcacggtca aga
//
LOCUS       DBMDTI010000359         1812 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_14017_length_1812_cov_1.630625, whole genome shotgun sequence.
ACCESSION   DBMDTI010000359 DBMDTI010000000
VERSION     DBMDTI010000359.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14017_length_1812_cov_1.630625"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            285..476
                     /locus_tag="ACZPB6_10580"
     CDS             285..476
                     /locus_tag="ACZPB6_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184944.1"
                     /translation="MAEDTNYVTLEVAIDSSLYDNLIHAAANRGQSVSEFVESVLDRE
                     VEQALSETPVEVRDPQDEE"
     gene            545..>1812
                     /gene="gpmI"
                     /locus_tag="ACZPB6_10585"
     CDS             545..>1812
                     /gene="gpmI"
                     /locus_tag="ACZPB6_10585"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR1184945.1"
                     /translation="MTTCARKKVLLMILDGWGIGRGDRADVIGSVRPQRLGEFAAKYP
                     HAELRTDGENVGLPDGQMGNSEVGHLNIGAGRVLYQDLVKINRAVADGSIGDNPEVKA
                     LIDYCREKGCALHVMGLLSDGGVHSLNSHMYAFLRLAKAAGLEKVYVHGFLDGRDTDP
                     KSGEGFVKDMLAEMAKPDTAGELVSLVGRYWAMDRDKRWERVRRAYDLLVKGVGNPVA
                     DMPDAVRVSYRQGITDEFLEPQYLTDAHGVAVGRIRPHDAVVFVNFRNDRAKELTTVL
                     TQEARPDLEMEPLPLYFCTMTPYDPTYKGLHVLFPKENVVNTIGEWVSAQGLRQLRIA
                     ETEKYAHVTFFLNGGREAPYEGEDRILVPSPSVATYDLQPQMSAPEVTEKLVEAMATE
                     QYDFICLNFANGDMVGHTGVYDAIEKAVVTV"
BASE COUNT          362 a          635 c          540 g          275 t
ORIGIN      
        1 tgaaccgaag ccctccgcgg aaccttccgc ggggggcttt ccttttcgtg tgcctccccc
       61 gctgcgcccg cttccgttcc gcaagagccc tctccggccc ctccaaaccg cgtctgcgag
      121 gcacctccag gttccgcggt attgacgatg gggagccgat ccgtgataat catccgattg
      181 atcaggaagg cggagacggc gcccgccgcg ccccccgaag ggaaagcgca gcacggaacc
      241 ccgatcccac gtccgcccgt ccaactattc gagagagaat tcaaatggca gaagacacca
      301 actacgtcac ccttgaagtg gccatcgatt cgtcccttta tgacaatttg attcatgccg
      361 ccgccaaccg cggccagtcc gtgagcgagt tcgtcgagtc cgtgctcgac cgcgaggtcg
      421 aacaggcgct gagcgaaacg ccggtcgagg tccgcgaccc gcaggacgag gagtaagccc
      481 cgcagggctc tcccaacaga caccaacgac agggattcag gatcaggaag aaggagacaa
      541 ggcgatgacg acctgtgcac gaaagaaggt tttgctcatg attctcgatg gctggggcat
      601 cggccgcggc gacagggccg acgtgatcgg cagcgtcagg ccgcagcgcc tcggcgagtt
      661 tgcggcgaaa tatccgcacg ccgaactgcg caccgacggc gaaaacgtgg gcctgcccga
      721 cggccagatg ggcaactccg aagtgggcca cctcaacatc ggcgccggcc gcgtcctcta
      781 ccaggatctc gtcaagatca accgcgcggt cgccgacggc tcgatcggcg acaatcccga
      841 ggtgaaggcc ctcatcgact actgccgcga aaagggctgc gccctgcacg tcatgggcct
      901 tctctccgac ggcggcgtgc acagcctcaa cagccacatg tacgcgttcc tgcgccttgc
      961 gaaggccgcg ggcctcgaaa aggtctacgt acacggcttc cttgacggcc gcgacaccga
     1021 tcccaagagc ggcgaaggct tcgtgaagga catgctcgcc gaaatggcga agcccgacac
     1081 tgcgggcgaa ctcgtgagcc tcgtgggccg ctactgggcc atggaccgcg acaagcgctg
     1141 ggagcgcgtg cgccgcgcct acgacctgct cgtcaagggc gtgggcaatc ccgtcgccga
     1201 catgcccgac gccgtgcgcg tctcctaccg ccagggcatc accgacgagt tcctcgagcc
     1261 gcagtacctc acggacgctc acggcgttgc cgtgggccgc atccgcccgc acgacgccgt
     1321 cgtcttcgtg aacttccgca acgaccgcgc caaggagctc acgaccgtgc tcacgcagga
     1381 ggccagaccc gatctcgaaa tggagccctt gccgctctac ttctgcacga tgacgcccta
     1441 cgacccgacc tacaagggcc tgcacgtcct cttcccgaag gagaacgtcg tcaacacgat
     1501 cggtgaatgg gtctccgccc agggcctcag gcagctgcgc atcgccgaga ccgaaaagta
     1561 cgcccacgtc acgttcttcc tcaacggcgg ccgcgaagcc ccgtatgaag gcgaggaccg
     1621 catcctcgtg ccgagtccct cggtcgcgac ctacgacctg cagccccaga tgagcgcccc
     1681 cgaggtcacc gaaaagctcg tcgaggccat ggccaccgag cagtacgact tcatctgcct
     1741 caacttcgcc aacggcgaca tggtgggcca cacgggcgtc tacgacgcga ttgaaaaggc
     1801 cgtcgtcacc gt
//
LOCUS       DBMDTI010000360         1784 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_14308_length_1784_cov_2.170529, whole genome shotgun sequence.
ACCESSION   DBMDTI010000360 DBMDTI010000000
VERSION     DBMDTI010000360.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14308_length_1784_cov_2.170529"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            527..>1784
                     /locus_tag="ACZPB6_10590"
     CDS             527..>1784
                     /locus_tag="ACZPB6_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HIR1184946.1"
                     /translation="MPTRTPKRALAAVFAAVALVTSVFAVPGVCAEPPADVVVGFVDR
                     PEPGYYSRTIMPTLAAMARAMPDRTVTGVRLSPDDPIADLVRLRPDILVGPASVYMEL
                     ELNYGGHAIATRKTRWADNPSASVGAAVVVRADNNEIRTLADLRGRRMASGGADAVDG
                     WLALAYELKERRQRPEAYRAHTTFLNFPMPDVISAVLSGKVEAGVLSTCMLERAELAR
                     LLKPGALRVVDEKTTQGFSCRHSTALYPDIVVAALPRTAPDVVKATTLALLSMPENAD
                     FAWQVATNFVSVNRLYKELHLGPWKHLDDWSWRNLWKSYGTYALIILGFLAWLVLDEI
                     RLKQTVRRRTAALTQALAEREKLEAQEAAARARLARIERTGAINQLCAMIAHELKQPM
                     SSVINYMTVLKIRLADRISEDDIVSRA"
BASE COUNT          325 a          652 c          501 g          306 t
ORIGIN      
        1 attctcatcc ttcgtcaatg ttggaatggg tttcttgtct gcatcaccgt tcctacctct
       61 cagaactttt aaacacagac cgcagagcgt gggactagga taaacatccc acggtgcctg
      121 tacattccaa accaacgtag ctcgcctccc agaggaggtt tgctgagact agtcaataaa
      181 ggctctttga agcctccccc tttaggcggg ggttattgac gatcatgcac gtgcgacacc
      241 gcacgccgct ccggaaacgc cggagcattt ccttctatct tagtaaaacg cgcgtctgaa
      301 agacaatccg cctgaaggcg catgacggcc gccccgccgc gcctcccccg ctcctcaggc
      361 tccgaagcga tgatacgaaa ttccctcttc ccctcgcgca gagacgacct ggaaaaaccc
      421 gcgggttaac gctcccgtcc gcggccctgt tgacgcggcg gcatcctgcc tacatagttc
      481 tcttgagaac acggcctttg ccgttgcacc acccgaaacc cgtcacatgc ccacacgaac
      541 tcccaagcgc gcccttgcag ccgtcttcgc cgccgtcgcc ctcgtcacca gcgtcttcgc
      601 cgttcccggc gtctgcgccg agccgcctgc ggacgtcgtc gtgggcttcg tcgacaggcc
      661 cgagcccggc tactactccc gcacgatcat gcccacgctc gccgccatgg cgagggcgat
      721 gcccgacagg accgtgacgg gcgtgcgtct ctcgcccgac gatccgatcg ccgacctcgt
      781 gcgccttcgg cccgacattc tcgtcggccc cgcttccgtc tacatggagc tcgagctcaa
      841 ctatggcggg catgcgatcg cgacgcgcaa gacgcgctgg gcggacaatc cgagcgcctc
      901 ggtgggcgcc gccgtcgtcg tgagggcgga caacaatgag atccgcacgc tcgccgacct
      961 ccgcggcagg cgaatggcct cgggcggcgc ggacgccgtc gacggctggc tcgcgctcgc
     1021 gtatgaactc aaggagaggc gccagcgccc cgaggcctac cgcgcgcaca cgaccttcct
     1081 caactttccg atgcccgacg tgatcagtgc ggtcctctcg ggcaaggtcg aggcgggcgt
     1141 gctctccacc tgcatgctcg agcgcgcgga gctcgcgagg cttctcaagc ccggagctct
     1201 cagagtcgtg gacgaaaaga ccacgcaggg cttttcctgc cggcacagca cggcgctcta
     1261 tcccgacatc gtcgtggccg cgctcccgag aaccgctccg gatgtcgtca aggcaacgac
     1321 gctcgcgctc ctctccatgc ccgagaacgc cgacttcgcc tggcaggtcg ccaccaactt
     1381 tgtctcagtg aacaggctct acaaggagct gcacctcggt ccctggaagc atctcgacga
     1441 ctggagctgg cggaacctct ggaagagcta cggcacgtac gcgctcatca tcctcggctt
     1501 cctcgcctgg ctcgtcctcg acgagatccg cctcaagcag accgtcaggc gccgcaccgc
     1561 tgcgctcacg caggccctcg ccgaacgcga gaagctcgag gcccaggagg ccgccgcccg
     1621 cgcccgcctc gcgcgcatcg agcgcacggg cgccatcaac cagctctgcg cgatgatcgc
     1681 acacgagctc aagcagccca tgagttcggt catcaactac atgaccgtac tcaagatcag
     1741 gctcgcagac cgcatcagcg aagacgacat cgtctcccgc gcgg
//
LOCUS       DBMDTI010000361         1777 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_14380_length_1777_cov_2.047733, whole genome shotgun sequence.
ACCESSION   DBMDTI010000361 DBMDTI010000000
VERSION     DBMDTI010000361.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14380_length_1777_cov_2.047733"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="ACZPB6_10595"
     CDS             <1..637
                     /locus_tag="ACZPB6_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184947.1"
                     /translation="KALMQFGFPRDRQLRYADFARLRPAQKRGRAFMYLKLATLYTKY
                     CRTYRPKFLRTVGMVAAVLGAGAVIQSAHMMPDWIPVWFLGVLAWNHSVVRAPDLHPN
                     VLFFLRDAGATTASSIPADLLRDKGPLLMLIWVVTGYMSGEWLSLNAGMTGFILMTFF
                     VSTFRIIRMMNGNLEAFVLVGEKVMEIAEDARADLAAQAREEQACREDEGS"
     gene            complement(698..838)
                     /locus_tag="ACZPB6_10600"
     CDS             complement(698..838)
                     /locus_tag="ACZPB6_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184948.1"
                     /translation="MNGVVMLVAVLVTLVALKQIIDRDWMGLLLTVVALLLVFGTGVR
                     RG"
     gene            complement(868..>1777)
                     /locus_tag="ACZPB6_10605"
     CDS             complement(868..>1777)
                     /locus_tag="ACZPB6_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184949.1"
                     /translation="TPEEEAGRLRNATDFKFDIRDVNINDSELPVFDLRFDAGLYTPP
                     NVWLPCLSAHFVLGSDMADLPGICPDGSMTDIFAASMQGNVEGVVRDLRLAWAGAELT
                     LKGSFVNHGLTGGKFETGVSFVDTGKGPVPGSVDALNRDLRQYVEALEKEGAVKRVGE
                     DAYETTLEFRLTPEGILKTTANGRDMDESKADFHWGLPSTEPSEIIIKISPKAEPYLT
                     KGVDAAVVEPIEKLLTGLDAVQRWSSTRNEYGAQVLVITVDDLARAPEVVEALDVRLE
                     QIKEVVQDAVVMEMRFAPEAMPAEWE"
BASE COUNT          342 a          584 c          489 g          362 t
ORIGIN      
        1 aaaggccctc atgcagttcg gctttccccg agatcgacag cttcgctacg cagacttcgc
       61 caggctccgt cccgcccaga agcgcggtcg cgcgttcatg tacctgaagc tcgccacgct
      121 ctacacgaag tactgccgca cgtaccgtcc caagttcctc aggaccgttg gtatggtggc
      181 cgccgtgctt ggcgccggcg cggtcattca gtccgcgcac atgatgcccg actggattcc
      241 cgtctggttc ctcggcgtcc tcgcctggaa ccactcggtg gtgcgtgcac ccgacctgca
      301 cccgaacgtc ctcttctttc ttcgtgatgc gggcgccacc acggcctcga gcattcctgc
      361 cgaccttctt cgtgacaaag gaccgctcct gatgctcatc tgggtcgtca caggctacat
      421 gtccggtgaa tggctgagcc tcaatgctgg catgacgggc ttcatcctca tgaccttctt
      481 cgtctcgacc ttccgcatca tccgcatgat gaacggcaac ctcgaggcgt ttgtcctcgt
      541 gggcgaaaag gtgatggaga tcgccgagga tgccagggcg gatctcgcgg cgcaggcgcg
      601 cgaggaacag gcatgccggg aagacgaagg cagctgatcg gccagttgag gatttgcgtc
      661 ccatgcattt caactgccat gtgataaatg attctggtca gcctcgccga acgccagtcc
      721 cgaaaacgag aagaagggca accacagtga gaagaagtcc catccagtcg cgatcgatga
      781 tctgcttcag agcaacaagc gtcaccagca ctgcgacgag catgacaaca ccgttcatga
      841 ctcacctctc acttgatatt ttgcatttca ttcccactcc gccggcattg cctcgggtgc
      901 aaagcgcatt tccattacga cagcgtcctg cacgacttcc ttgatttgct caagccgaac
      961 gtccagcgct tccacgacct cgggcgccct ggcgaggtca tcgaccgtga tcaccagcac
     1021 ctgtgccccg tactcattcc gggtcgacga ccagcgctgc acggcatcaa gtcccgtgag
     1081 cagcttctcg atcggctcga caacggcggc atccacgccc ttcgtcagat acggttcggc
     1141 cttgggcgaa atcttgatga tgatttcgct gggctcagtc gagggcagcc cccaatggaa
     1201 gtcggccttg ctctcgtcca tgtcccgtcc gttggcggtc gtcttgagaa tgccttcggg
     1261 cgtgaggcgg aattcgagcg tcgtttcgta ggcatcctcg ccgacccgtt tcacagcgcc
     1321 ctccttctcc agcgcttcga catactgacg cagatcgcgg ttgagcgcat caaccgaacc
     1381 cggcaccggt cctttgccgg tatcgacaaa agagaccccg gtctcaaact ttccgccggt
     1441 gagcccatgg ttgacgaagc tgcccttgag cgtcaattcg gcgccggccc aggctaaccg
     1501 gaggtcacgg accaccccct ccacattccc ctgcatcgag gcggcgaaaa tgtccgtcat
     1561 gcttccgtcg ggacaaatgc cgggcaggtc cgccatatcc gagcccagta cgaaatgcgc
     1621 tgagaggcac ggcagccaga cattcggagg cgtgtacagg ccggcatcaa accgcaagtc
     1681 gaaaaccggc agctccgaat cgttgatgtt gacgtcccgg atgtcgaact tgaagtcggt
     1741 cgcattacgc aggcggcctg cctcctcctc aggcgtc
//
LOCUS       DBMDTI010000362         1777 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_14381_length_1777_cov_1.988067, whole genome shotgun sequence.
ACCESSION   DBMDTI010000362 DBMDTI010000000
VERSION     DBMDTI010000362.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14381_length_1777_cov_1.988067"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            311..1744
                     /locus_tag="ACZPB6_10610"
     CDS             311..1744
                     /locus_tag="ACZPB6_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024774.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1184950.1"
                     /translation="MPGRHRRCSNTPSFFGLGEMMNVPGILNGNPKACSKVAAALNLD
                     KPVDGHAPLCTGADLEAYARAGIRTDHECTTTAELRERISLGMYVSIREGSLARNLLP
                     LIENLTADLSRRCSFCTDDRHAADTVERGHINGMLAMAVKAGIRPVDALRMATLNTAE
                     CYGLQHRGVIAPGRRADLLFLDNLDDFSSLAVWTKGRLVARNGSLVIGAPKQKPGFLA
                     DTVRIAPVTAEAFDFKAPSEKARMIGLLPHSLITEEKIAAVKTLRDGTVRLSDNPGCV
                     KLAVVERHRRTGHIGTCLLDPAHGLRGGAIATSVSHDSHNIVVAGEDEEDMALAVQKI
                     EEMQGGIVMVARGEVLDALPLPVAGLISDGTPEETAAAIRRLTKLAHGHYGISQDSDA
                     FMTLWFLALPVIPYLKLTTKGLFDVTRFDLVPNDAALEDNRGRVGDRISSRPAAFFAC
                     RHAFPVIRAFSSYFLAFPRVHLSSFVI"
BASE COUNT          351 a          564 c          511 g          351 t
ORIGIN      
        1 aagacggcct catccacggg ttcggttcgg aacccgcaaa agaaactttc gaagcaaagg
       61 gcgctgtgct tctgccgagc ctcatcgact cgaacgtgca catcgagagc ccgatgctct
      121 cccccgccag cttcgcctca ctggttcttc cgttcggtac gacgactgtc accgccgacc
      181 ctcatgaaat gaccaacgta cgccatgcac gggctcgtcg aggcctgccg ggacctcccg
      241 cttgtcgtga agctcatggt gccgtcctgt gttccggccc tgcccttcga ggatgaaggc
      301 gctttcattg atgccgggca gacacaggcg ctgctcgaac accccgagtt ttttcggtct
      361 tggcgaaatg atgaacgtgc ccggcatcct caatggcaac cccaaggcct gcagcaaggt
      421 cgcggctgcc ctaaatctcg acaagcccgt cgacggccac gcgccgcttt gcacgggcgc
      481 ggatctcgaa gcctatgccc gagcaggcat ccgcacggac catgaatgca cgacgactgc
      541 ggagctcagg gaacgcatct cgcttggcat gtacgtctcg atcagggaag gctcgctcgc
      601 gagaaacctt ctgcccttga tcgagaacct cactgccgac ctctcgcggc gctgcagctt
      661 ctgcaccgac gacaggcacg ccgccgacac ggtcgagcgc ggtcacatca acggcatgct
      721 ggcgatggcc gtcaaggcgg gcatccgtcc cgttgacgcc ttgaggatgg caacgctcaa
      781 taccgccgaa tgctatggcc ttcagcaccg cggcgtgatt gcgcccggac gcagagccga
      841 cctgctgttt ctcgacaacc ttgatgactt cagttcgctc gccgtctgga cgaagggcag
      901 gctcgtcgcg cgcaatggga gccttgtcat cggtgcgcca aagcagaagc ccggatttct
      961 cgcggacacg gtgaggattg cgcccgtgac ggccgaagcc ttcgacttca aagccccgtc
     1021 cgagaaagcc aggatgatcg gccttctgcc tcacagcctc atcacggagg agaagattgc
     1081 tgcggtgaag acacttcgcg atggtacggt gaggctctcc gacaatccgg gatgcgtgaa
     1141 gcttgcggtc gtcgaacgcc acaggcgcac aggccacatc ggcacgtgtc ttcttgatcc
     1201 cgcccacgga ctcagaggcg gcgcaatcgc cacgtcggtt tcccacgact cgcacaacat
     1261 cgttgtggca ggtgaggacg aagaggacat ggcgctcgcc gttcaaaaga ttgaggaaat
     1321 gcagggcggg attgtcatgg ttgcgagggg agaagtactc gacgcgctgc cgcttcccgt
     1381 cgcgggcctc ataagcgatg ggacacccga agagacggcg gcggccatca ggcggctcac
     1441 gaagctcgcc cacgggcact acggcatttc gcaggacagc gacgccttca tgaccctctg
     1501 gttccttgcg ctccccgtta ttccgtacct caagctcacg acgaagggac ttttcgacgt
     1561 cacccgattt gatttagtgc caaacgacgc ggcgcttgaa gacaatcgcg gacgagtggg
     1621 agaccggatt tcatcccgac ccgccgcatt ttttgcctgc cgtcatgcat ttccggtgat
     1681 tcgagcgttt tcttcgtatt ttttggcttt ccctcgtgta cacttgagct cattcgttat
     1741 ttaagcaaga tcaggaacat gtccaacgtc ttcatca
//
LOCUS       DBMDTI010000363         1777 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_14382_length_1777_cov_1.936754, whole genome shotgun sequence.
ACCESSION   DBMDTI010000363 DBMDTI010000000
VERSION     DBMDTI010000363.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14382_length_1777_cov_1.936754"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            191..1342
                     /gene="earP"
                     /locus_tag="ACZPB6_10615"
     CDS             191..1342
                     /gene="earP"
                     /locus_tag="ACZPB6_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HIR1184951.1"
                     /translation="MSQRAESADTVLAPAGAVIFCRVIDNFGDAGVTWRLSKRLRDLG
                     LPVTLVIDAVDVLARLVHDLDPAATDALVRGIRIADWDAFEAEVSAGDLSRIGGVWPS
                     LVFETFGCRLPDAVEAGLCDGRTRLWVNLDYLSAEEWVEGSHMVWGLHPTLPVKKLWF
                     FPGFTDRTGGLLIEDGLDEARRAFDAAAWRRAHGARDGLRTLFFFAYPVNAIDRLAAG
                     LRAAGEPLNLMLAPGEASDMLERALKGDARFNVVRLPFVPQEEFDDILRAVDGAVIRG
                     EDSFVRAQLAGTPLLWATYPTEDKAHEIKLDAWLARFREAWPADAAVPFETYEAASRH
                     WVDGTLEPAEVKAWLDQLPVQKRAAEVWRESLLRRGDLARRILELVPRA"
     gene            1523..>1777
                     /locus_tag="ACZPB6_10620"
     CDS             1523..>1777
                     /locus_tag="ACZPB6_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR1184952.1"
                     /translation="MKTAQELRLGNVFMVGKDPMVVVKSEYSKGGRGASTVKMKMKNL
                     LTNAMTETVFRADDKFEDLILERKEVTYSYFADPHYVWMDE"
BASE COUNT          312 a          626 c          528 g          311 t
ORIGIN      
        1 aagacacacg cctttcatcg gggacaacca aatagatcgg aagagagagg tttgccgcat
       61 tcctccatcc gaatgaattc ggaggcttcc tgcggctatt tctgtgaacg tccgatgatt
      121 ttaacgcgcg cccgcgcgct acaatgcgcg cacgttcaac tcgttacgct tctgcaaagg
      181 cttcagcacc atgtcccaaa gagccgaatc cgccgacacc gtccttgcgc ccgcaggcgc
      241 cgtcatcttc tgccgcgtca tcgacaactt cggcgacgcg ggcgtcacct ggcgactctc
      301 caagcgcctg cgcgacctcg gcctgcccgt cacgctcgtc atcgacgccg tcgacgtgct
      361 cgcgaggctc gtgcacgacc tcgaccccgc cgccacggac gcgctcgtgc gcggcatccg
      421 catcgccgac tgggacgcct tcgaggccga ggtttcggca ggcgacctct cccgcatcgg
      481 cggcgtctgg ccctcgctcg tcttcgagac cttcggctgc cgcctgcccg acgccgtcga
      541 ggcaggtctc tgcgacggcc gcacccgcct ctgggtcaat ctcgactacc tctccgccga
      601 ggaatgggtc gagggctcgc acatggtctg gggcctgcat ccgacgctgc ccgtcaaaaa
      661 gctctggttc ttcccgggct tcaccgacag gacgggcggc cttctcatcg aggacgggct
      721 cgacgaggcc cgcagggcct tcgacgcagc cgcctggcgc cgtgcgcacg gtgcaaggga
      781 cgggcttcgc acgctcttct tcttcgccta ccccgtcaac gcgatcgacc gccttgccgc
      841 cggcctcagg gccgcgggcg agcccctcaa cctgatgctc gcgccgggcg aggcctccga
      901 catgctcgag cgcgccctca agggcgacgc ccgcttcaac gtcgtgcgcc tgcccttcgt
      961 gccgcaggag gagttcgacg acatcctgcg cgccgtcgac ggcgcagtca tccgcggcga
     1021 ggacagcttc gtgcgcgccc agctcgcggg gacgccgctc ctctgggcca cctacccgac
     1081 cgaggacaag gctcacgaga tcaagctcga cgcctggctc gcaaggttca gggaggcctg
     1141 gcctgcggac gccgccgtcc ccttcgagac ctacgaggcc gcctcccgcc actgggtcga
     1201 cggcacgctc gagcccgccg aggtgaaggc ctggctcgac cagctgcccg tccagaagag
     1261 ggccgccgaa gtctggcgcg agtcgcttct caggcgcggc gaccttgccc gccgcatcct
     1321 cgaactcgtc ccgcgcgcct gaggcgcagg caggcgtcag ggctgcaggg gcgcctccgc
     1381 atcaaaaccc gggcccaaat taacagttca agggccggaa aatttggtag aatcgtctgc
     1441 tattgatttt ttgcttcttg cgccctcttg caaacgggcg cgggaaaaat tctcgcaaca
     1501 ctgcaattag ggaatctgat aaatgaaaac cgctcaggaa ctccgcctcg gcaacgtctt
     1561 catggtcggc aaggacccga tggtcgttgt caagtccgaa tactcgaagg gtggccgcgg
     1621 tgcctccacg gtcaagatga agatgaagaa ccttctcacg aacgccatga cggaaaccgt
     1681 tttccgtgct gacgacaagt ttgaagacct catcctcgaa cgcaaggaag tgacgtactc
     1741 ctacttcgcc gatccgcact atgtctggat ggacgag
//
LOCUS       DBMDTI010000364         1774 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_14411_length_1774_cov_1.979079, whole genome shotgun sequence.
ACCESSION   DBMDTI010000364 DBMDTI010000000
VERSION     DBMDTI010000364.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14411_length_1774_cov_1.979079"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="ACZPB6_10625"
     CDS             complement(<1..1511)
                     /locus_tag="ACZPB6_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR1184953.1"
                     /translation="MEPFNAGWLSIVPPIVAIALALITKEVLSSLVLGILTGTLIYTI
                     GMDGNVLMGTLESAFTVMGNKVDFNILIFCSLLGALVYVVAMSGGTKAYGNWAVKRIK
                     GRKSALAATTGLGALIFIDDYFNCLTVGTVMRPICDRYNIARAKLAYIIDSTAAPVCI
                     IAPVSSWAAAVGSSLQGSTAFESDMAAFIATIPWNFYALLCLLLIVFIVVTDFDFGPM
                     RKSEERAAKGEQFTSTAATSSAEPPNANPHGTLWDMLVPIASLIVFCVLALMYSGGYW
                     GADPKYHTFASALGNSSAAPSLVWASFGAVGIAFLMYVPRGLVPFTDFMSGLVEGMKL
                     MLPANIILVLAWTLSGVCRDLLQTPLFVQSIVTSGGAEWSIFLPAVIFVIAAFLSFST
                     GTAWGTFGILIPIVVPVVEAIDPGLTVVALSATLAGSVFGDHCSPISDTTILSSAGSG
                     CNHIEHVSTQMPYSLTVAFSACLGYVVAGLTGGNWILSITTAVVALIGTVLLL"
BASE COUNT          368 a          500 c          628 g          278 t
ORIGIN      
        1 tgaagcagca ggacggtgcc gatgagcgca acgacggcgg tcgtaatcga gaggatccag
       61 ttgccgcccg tcaggcccgc gaccacgtag cccaggcagg cggagaacgc caccgtgagg
      121 ctgtagggca tctgggtcga gacgtgctcg atgtggttgc atcccgagcc tgccgacgag
      181 agaatcgtcg tgtccgaaat gggcgagcag tggtcgccga agacggagcc cgcgagcgtt
      241 gcggagagcg cgacgacggt gaggcccgga tcgatggcct cgacaaccgg caccacgatc
      301 gggatcagga tgccaaacgt gccccaggcc gtgcccgtag agaaggacag gaaggcggcg
      361 atcacgaaga tcacggccgg caggaagatc gaccactcgg cgccgcccga ggtcacgatg
      421 gactgcacga agagcggggt ctggagaagg tcgcggcaga cgccggagag cgtccaggcg
      481 agcaccagga tgatgttggc cggaagcatg agcttcatgc cttcgacgag gccgctcatg
      541 aagtccgtga acggcacgag accgcgcggg acgtacatca ggaaggcaat gccgacggcg
      601 ccgaaggagg cccagacgag ggacggcgcg gcggaggagt tgcccagcgc ggaggcgaag
      661 gtgtggtact tcggatcagc gccccagtag ccgcccgagt acatcagcgc gagcacgcag
      721 aagacgatca gggaggcgat gggcacgagc atgtcccaga gcgtgccgtg cggattggcg
      781 ttgggcggct cggcggagga cgtagcggcg gtcgacgtga actgttcccc cttggcggcg
      841 cgctcctcgc tcttgcgcat cgggccgaag tcgaagtcgg tcacgacgat gaagacgatg
      901 agcagcaggc agagcagcgc gtagaagttc cagggaatcg tggcgatgaa cgcggccatg
      961 tcgctttcga aggccgtgga gccctgcagt gaggagccga cggcggcggc ccagctcgaa
     1021 acgggcgcga tgatgcacac cggagcggcc gtcgagtcga tgatgtaggc gagcttcgca
     1081 cgggcgatgt tgtagcggtc gcagatcgga cgcatcaccg tgcccaccgt gaggcagttg
     1141 aagtagtcgt cgatgaagat gagcgcgccg agacccgtgg tggctgccag ggcgctcttg
     1201 cggcccttga tgcgcttgac ggcccagttg ccgtaggctt tcgtgccgcc cgacatggcg
     1261 acgacgtaga cgagggcgcc gagaagcgag cagaagatca ggatgttgaa gtcgaccttg
     1321 ttgcccatca cggtgaacgc gctttcaagc gtgcccatca ggacgttgcc gtccatgccg
     1381 atcgtataaa tgagggtacc cgtcagaatg cccagaacga gggaggagag aacctccttc
     1441 gtaatgagcg caagagcaat ggccacgatc ggcgggacga tcgaaagcca tccggcgtta
     1501 aacggttcca tgagaatcgc tgtcctttgg ttttcgtgcg gcaggacccc ctgtcccgga
     1561 gccctgcggc ccgcatgcgc ctctctttga ccgcacgcag gcagctgatg ccgctgtttg
     1621 tcattgggtt gtaagtcctt caatcctctt ccccgcaggt gtcggtcccg cgaggacgta
     1681 gtggcggatt ttaggctgat gcgaagtgta gcaaggcgta acagacttca acgtgaaaaa
     1741 acagccgcgc cgcgttttgc acgacgcttt taga
//
LOCUS       DBMDTI010000365         1764 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_14545_length_1764_cov_1.909200, whole genome shotgun sequence.
ACCESSION   DBMDTI010000365 DBMDTI010000000
VERSION     DBMDTI010000365.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14545_length_1764_cov_1.909200"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="ACZPB6_10630"
     CDS             <1..254
                     /locus_tag="ACZPB6_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HIR1184954.1"
                     /translation="GAFEPAAGWTLQPSVDFTVVPQLGDKDVDTVLGSVDVIDNLYNT
                     ELGVEAATGNFSFGLSYKYGFGKEDRKNQSLNLNARYMF"
     gene            547..975
                     /locus_tag="ACZPB6_10635"
     CDS             547..975
                     /locus_tag="ACZPB6_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024276.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HIR1184955.1"
                     /translation="MINRRRFIQASALLIAGTSTLALTGCGSPKTPEDVACAFVRALY
                     RGDAAEATKFFHVPADAKDAEVKIAEAKISESAAQAKDSASKQGGLRDVSIMEPVAKE
                     AIEAGKCRARVKVAFGNGSSKVETAQLIKTEKGWLVTFGL"
     gene            986..1687
                     /locus_tag="ACZPB6_10640"
     CDS             986..1687
                     /locus_tag="ACZPB6_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184956.1"
                     /translation="MNTRTAALLTAAAVLMSTGTVLMHEDVKGELIDGATGLLPGLQE
                     APSAEVPTGVTLDDLAPCGSLRTGDLVFRRGFGTESRLIIEAQGPNRAPFSHVGMVVS
                     EHPVMIVHATTSDDPAHPNSVISSSLPEFLSMGDMAGVARPAWSDALKASAAGRARGM
                     TGVAFRLVPDDPDALYCTTLIEKSFEPDLKLDLPRDVVKVPVVGGAYLFPAALWEADG
                     MTHVCMIGKHKTSKP"
BASE COUNT          344 a          601 c          481 g          338 t
ORIGIN      
        1 ccggtgcgtt cgaaccggcc gccggctgga ccctccagcc gtccgtcgac ttcacggtcg
       61 ttccgcagct cggcgacaag gatgtcgata cggtcctcgg ttccgttgac gtcatcgaca
      121 acctctacaa caccgaactc ggcgttgagg ctgccaccgg caacttctcg ttcggtctga
      181 gctacaagta cggtttcggc aaggaagacc gcaagaacca gtcgctcaac ctgaacgccc
      241 gctacatgtt ctgatagatc cccccgcatg gagccttccg gctcctgcgg atgatcaaat
      301 ctgaacgcaa gcctgaggcc ccgcgtcctt caccggatgc ggggtctttt tactgtctcc
      361 attcggagtt cccccggctc tgcctcaacg cctcgtcacc gtccctcttg cgtttgaata
      421 cggttcggaa atcatgccgt cccgcatttc gcgttgcttt taggtagact tcggaaaaac
      481 cccgcacgtc ctcagacttg tcaatcgacg tgccgggcca aagctaaaat agaggagaaa
      541 accatcatga tcaaccgccg ccgcttcatc caggcgtccg ccctgctcat cgccggcacc
      601 tccacgctcg ccctcacggg ctgcggaagc cccaaaacgc ctgaagacgt cgcgtgcgcc
      661 ttcgtgcgcg ccctttaccg cggcgatgcc gccgaagcca cgaagttctt ccacgttccc
      721 gccgacgcca aggacgccga ggtgaagatc gccgaagcca agatcagcga gagcgccgct
      781 caggccaagg actccgcctc caagcagggc ggtcttcgcg acgtctccat catggaaccc
      841 gtcgccaagg aagccatcga ggccggcaag tgccgtgccc gcgtcaaggt ggctttcggc
      901 aacggctcga gcaaggtcga gaccgcccag ctcatcaaga ccgagaaggg ctggctcgtc
      961 accttcggcc tctgattcca ctctcatgaa tacccgcacg gcggctctcc tcacggcagc
     1021 cgccgttctc atgtccacag ggaccgtcct catgcatgag gacgtcaagg gtgagctcat
     1081 cgatggcgcg accgggcttc ttccgggact tcaggaggca ccgtccgccg aagtccccac
     1141 gggcgtcacg cttgacgacc tcgccccctg cggcagtctc aggaccggcg atctggtctt
     1201 tcgcaggggc ttcggaacgg agagccgcct catcatcgag gctcagggtc ccaacagggc
     1261 gcccttctct cacgtcggca tggttgtttc cgagcatccc gtgatgatcg tgcacgccac
     1321 gacatccgac gatccggcac acccaaacag cgtcatcagc tccagccttc ctgagtttct
     1381 ctccatgggc gacatggcgg gcgttgcgag acccgcctgg agcgatgctc tcaaggcatc
     1441 agctgcagga cgtgcaagag gcatgacggg cgtagccttc agacttgtgc cggacgatcc
     1501 cgatgcactc tactgcacga cgctaattga aaagtccttc gagcctgatc tcaagcttga
     1561 ccttccccgc gacgtcgtca aggtccccgt tgtgggcggc gcgtatctct tccctgccgc
     1621 gctctgggag gcggacggca tgacgcatgt ctgcatgatc gggaagcata agacgagcaa
     1681 gccgtgactc tcgtcgtccg agcctgtaga atcttctgac catctcagaa tcaaaaccaa
     1741 ccaggcaatc atttctataa aggc
//
LOCUS       DBMDTI010000366         1761 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_14580_length_1761_cov_2.255422, whole genome shotgun sequence.
ACCESSION   DBMDTI010000366 DBMDTI010000000
VERSION     DBMDTI010000366.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14580_length_1761_cov_2.255422"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            207..506
                     /locus_tag="ACZPB6_10645"
     CDS             207..506
                     /locus_tag="ACZPB6_10645"
                     /inference="COORDINATES: protein motif:HMM:NF040101.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6429 family protein"
                     /protein_id="HIR1184957.1"
                     /translation="MQEQKNNEDTLTDDAIEAGIEELTLALLYLKRFKWNHDDQVARA
                     SWRSFDWETLDNLLQSSDLSGCDHKAVWISDEGIRRARNILEKYGLSHLEGAAEA"
     gene            506..1417
                     /gene="rarD"
                     /locus_tag="ACZPB6_10650"
     CDS             506..1417
                     /gene="rarD"
                     /locus_tag="ACZPB6_10650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIR1184958.1"
                     /translation="MTKRLEPDFRLGLTGAIVSYLSWGLMPIYWNLLEGAGSVEVIAH
                     RILWSLLFIPLVMAVRGKLRSAWSDIKSIFTSPVTALLLATAACFAGLNWWINVIAVL
                     SGHVVELGIGTFLTPLISVSCGVLFFHERLSRTKTAGVMLAMTGVLIMIVSFGHFPWI
                     SLGVSAIWGIYGALKKKLMLDAWVSILLEALMMMPVAFGYVFWLSSIGENHFLPTVNP
                     ELSLCLAGTGIVASIPLIFFTIAAVNLPMNVLGFCQYISPILTLLLGILFFNERFGWA
                     ELFPLLFIWSGIAAFLFAEVREHRKAH"
     gene            1599..1691
                     /locus_tag="ACZPB6_10655"
     CDS             1599..1691
                     /locus_tag="ACZPB6_10655"
                     /inference="COORDINATES: protein motif:HMM:NF015424.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184959.1"
                     /translation="MTTTSAKALGLKGAKAYAMIKASNVMLAVD"
BASE COUNT          353 a          550 c          441 g          417 t
ORIGIN      
        1 agtagaaaga tcgcgtttat ttgcgtccag atgaacatga aaatcctttc tttagctgtg
       61 cgacaaggag agagaggcat ttgagaacgt gaaaaaatag gcccttttgg gcttccgggc
      121 tctgaaaacg cctcgttcgg gcacgttttc gccacagcag gtagaatgtc gagatagcaa
      181 aaacaaaaca aagccaggaa gcctccatgc aagaacaaaa aaacaatgaa gacactctga
      241 ctgacgatgc cattgaggcc ggcatcgagg aactgaccct cgcgctcctt tatttgaagc
      301 gcttcaagtg gaaccatgac gaccaggtcg cccgtgccag ctggcgtagc ttcgactggg
      361 agacgctcga caatcttttg cagtcctccg atctttccgg ttgtgaccac aaggcggtct
      421 ggatttccga cgaaggcatc aggcgggcgc gcaacattct ggagaagtac ggcctttctc
      481 atcttgaagg tgctgcagaa gcttaatgac caaacgactg gagccggact tccggctcgg
      541 acttaccgga gccatcgtct cctatctcag ctgggggctc atgcccatct actggaacct
      601 gctcgaagga gccggctccg tagaggtgat tgcccatcgc attctctggt cgcttctctt
      661 catcccgctc gtgatggcgg tccgcggaaa actccgctcc gcctggtccg acatcaagtc
      721 gattttcacc tcccccgtca cagcccttct gctcgcgacc gccgcgtgct tcgccggtct
      781 caactggtgg ataaacgtga tcgccgttct gagcggtcac gttgtggagc tcggcatcgg
      841 cactttcctc acgccgctca tctccgtttc ctgcggtgtt ctctttttcc atgaacgcct
      901 ctcgcgcacg aagaccgctg gcgtgatgct cgccatgacc ggcgtcctca tcatgatcgt
      961 ctccttcggc cacttcccct ggatttcgct cggcgtctcg gcgatctggg gaatttacgg
     1021 ggcgctcaag aaaaagctca tgctcgacgc ctgggtgagc attctgcttg aagccctcat
     1081 gatgatgccc gttgctttcg gttatgtctt ctggctttcg tcgatcggtg aaaatcactt
     1141 cctgccgacg gtcaatcccg agctttcgct ctgtctcgcc ggcaccggca tcgtcgcctc
     1201 catcccgctt atcttcttta cgatcgcagc ggtcaacctc ccgatgaacg tgctcggatt
     1261 ctgccagtac atctcgccga tcctcacgct tttgctcgga atcctattct tcaacgagcg
     1321 tttcggctgg gccgaactct tccccctcct cttcatctgg agcggcatcg ctgccttcct
     1381 ttttgcagaa gttcgcgaac atcgcaaggc ccattgatca cttcggatat tcttgctgaa
     1441 tgcacgggag ccgcacaaca tacgcatctt tcacaggaga ttcccttgaa gatttccgcc
     1501 cgcaactgca ttgaaggcga ggtcgtcgcg atccacgatg gccccgtctc cacggaagtg
     1561 acggttgcaa cgccgccggc gaacgcatcg tctcctctat cacgacgacg tccgccaagg
     1621 ccctcggcct caagggcgcc aaggcctacg cgatgatcaa ggcctccaac gtcatgctcg
     1681 ccgtcgactg atcgaattat tcaacgaatt caccctcttc atcatcgtcc gccaggtgtc
     1741 caaagccacc cagatcggcc t
//
LOCUS       DBMDTI010000367         1742 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_14802_length_1742_cov_2.169409, whole genome shotgun sequence.
ACCESSION   DBMDTI010000367 DBMDTI010000000
VERSION     DBMDTI010000367.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14802_length_1742_cov_2.169409"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="ACZPB6_10660"
     CDS             <1..342
                     /locus_tag="ACZPB6_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184960.1"
                     /translation="ELDFPRLEVRAMGAMGVRKDVKTFADFDVGVQIHPLDDELMKTR
                     PDVVAVHDFVKDLVDSKALACRVKDECRARVTVEPDERGFGIIRSNGVDVTEKVMKFI
                     FAEPAGTGEAG"
     gene            471..>1742
                     /locus_tag="ACZPB6_10665"
     CDS             471..>1742
                     /locus_tag="ACZPB6_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1184961.1"
                     /translation="MVSRIEVRGARVHNLRNVDVDIPLNKIVGVAGVSGSGKSSLALG
                     VLYTEGSRRYLEALSTYTRRRLTQAPRADVDEVLHVPAALALHQRPGVPGIRSTFGTG
                     TELLNTIRLMFSRLAFHRCPNGHMVGPTIDVAAEKALVCPVCGAKFFAPGAEDLAFNS
                     GGACRTCDGTGMVRSVDRATLVPDETLSIDEGAVMPWRTLMWSLMTEVCRSMGVRTNV
                     PFRELTPEEKAIVYDGPMVKKHIFYHTKGSNDAAELDFTYFSAVKTVENALAKVKDES
                     GMKRVERFLRLDVCPDCRGSRFSEAARAPKIMGVSIDEVSRMPLVDLCAWVDRVPASL
                     PPEMRPMAESLCEAFGHTAARLLDLGLGYLSLDRAASTLSTGERQRMQLARAVRNRTT
                     GVLYVLDEPSIGLHPANVEGLKAVMYDLVADG"
BASE COUNT          307 a          526 c          594 g          315 t
ORIGIN      
        1 gagcttgact tcccgaggct tgaagtgcgc gccatgggcg cgatgggcgt gaggaaggat
       61 gtgaagacgt tcgccgactt cgatgtcggc gtccagattc atccgcttga cgacgaactc
      121 atgaagaccc gtcccgacgt cgtcgccgtg cacgacttcg tgaaggacct cgtcgactcg
      181 aaggcgctcg cctgccgcgt gaaggacgag tgccgcgccc gcgtgacggt cgaacccgac
      241 gaaaggggct tcggcatcat caggtcgaac ggcgtcgacg tgacggagaa ggtcatgaag
      301 ttcatttttg ccgaacccgc cgggacgggc gaagcgggct gacgtccaca ttgatccgtt
      361 ctgcctggcc gatcgggaac ggcccgtctt ccgtcgcgaa ggcgggcctt ttgggcggag
      421 catcggacgt atgattgagt acgtctttcc aaaaaaagga gcacagcaca atggtgagca
      481 ggatcgaagt gcgcggcgcg cgcgtgcaca atctcaggaa cgtcgacgtc gacatcccgc
      541 tcaataaaat cgtcggcgtg gcgggcgtct ccgggtccgg caaatcatcg ctcgcgctcg
      601 gcgtcctcta caccgagggc tcgcggcgct accttgaggc gctctcgacc tatacgcgcc
      661 ggcggcttac gcaggcgccg cgggccgatg tcgacgaggt gctccatgtt cccgcagcgc
      721 ttgcgctcca tcagagaccg ggcgtgcccg gcatccggag taccttcgga acggggaccg
      781 agctgctcaa cacgattcgc ctcatgtttt cgcgccttgc gtttcacagg tgtccgaacg
      841 gccacatggt cgggcccacg atcgacgtcg cggccgaaaa ggcgctcgtc tgtccggtct
      901 gcggggcgaa gttctttgcg ccgggtgcgg aggatctggc cttcaacagc ggcggcgcct
      961 gcaggacttg cgacggcacg ggcatggtga ggtcggtcga cagggcaacg ctcgttcccg
     1021 acgagacgct ttcgatcgac gaaggcgccg tgatgccctg gcgcacattg atgtggtcgc
     1081 tcatgaccga ggtctgtcga tcgatgggcg tgaggacgaa cgtgcccttc agagagctga
     1141 cgcccgagga gaaggcgatc gtctacgacg gcccgatggt gaagaagcac atcttctatc
     1201 atacgaaggg ttcgaacgat gcggcggagc ttgatttcac gtacttctcg gccgtaaaga
     1261 cggtcgagaa cgcgctcgcc aaggtgaagg acgagtcggg catgaagcgc gtcgagcgtt
     1321 tcctgagact tgatgtatgc cctgactgca ggggctcaag gttctcggag gccgcgcggg
     1381 cgccgaagat tatgggcgtc tcgatcgacg aagtctcccg catgccgctc gtcgacctgt
     1441 gcgcttgggt cgaccgcgtg cccgcgtcgc tcccgcccga gatgcggccg atggcggaga
     1501 gtctctgcga ggcgttcgga cacacggccg cgaggcttct ggatctcggg ctcggatacc
     1561 tgtcgcttga tcgtgcggcc tcgacgctct cgacgggcga gcggcagcgc atgcagctcg
     1621 cgagggccgt gcgcaaccgc acgacgggcg tgctctacgt gctggacgaa ccttcgatcg
     1681 ggctccatcc cgccaacgtc gaggggctca aggccgtgat gtacgacctg gtggcggacg
     1741 gc
//
LOCUS       DBMDTI010000368         1734 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_14903_length_1734_cov_1.697489, whole genome shotgun sequence.
ACCESSION   DBMDTI010000368 DBMDTI010000000
VERSION     DBMDTI010000368.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14903_length_1734_cov_1.697489"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(120..1085)
                     /locus_tag="ACZPB6_10670"
     CDS             complement(120..1085)
                     /locus_tag="ACZPB6_10670"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1184962.1"
                     /translation="MSEPATLPMRRVLQMDGETLAFFLVLYRTESLPKAAAEMNISLS
                     SANRQLARLRKAWADPLFVRSGLLMKATPAAARRYDRVQRVVREIEALTLDEEVQPAL
                     LKQIVRIATYDNAFAVGLATVFGELQTKLPGVQFRAVQADEHMFEDLRADRLDMAFYA
                     RQGVQPGIHSTPLFTTPYACVTRRGHPLEKVVERHGHLDREDLARFRTVLVNAQPDRN
                     REPNSPANGWFNPPSADKVAMVLPFFLAAPLALPGTDCYAVIPAASARFAFETEKFSI
                     LPFGPGAPELTVRLGWHERTHADPACQLIRSVLIEAVRKKAAALL"
     gene            1230..>1734
                     /locus_tag="ACZPB6_10675"
     CDS             1230..>1734
                     /locus_tag="ACZPB6_10675"
                     /inference="COORDINATES: protein motif:HMM:NF016121.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR1184963.1"
                     /translation="MQLKSSVLSAAVKSALVAAGLAAGVASAMTPGAYEATAQGRNAD
                     ITVRVTVSADKIEKIEIVKHSETPGIGDLAVNELPAAMLSAQTAAVDAVGGATMSSAA
                     IKKAVAEALRKAGASEFLTAPKAGADKAAAADFKTSADVIVVGAGGAGMVAAATAVDN
                     GATVIVLE"
BASE COUNT          303 a          551 c          581 g          299 t
ORIGIN      
        1 gcctctcgac gaggtcttca ccatcgacga ggagccgcag gacgttcagg cggaacagac
       61 cgcccgtccc gcctgacggg gacctgcagg cagactgcct tcatgggcgg ccgcgcttct
      121 cagaggagtg cggccgcctt ttttctgacg gcctcgatga ggacggacct gatgagctgg
      181 caggcgggat cggcgtgcgt gcgctcgtgc cagccgagcc tgacggtgag ctcgggagcg
      241 ccgggtccga aggggagaat cgagaatttt tcggtctcga aagcaaaccg cgcggatgcg
      301 gcgggaatga cggcgtagca gtcggtgccc ggaagcgcca gaggcgcggc gagaaagaac
      361 ggaaggacca tcgcgacctt gtcggccgac ggcggattga accagccgtt cgccggactg
      421 ttgggctctc tgttgcggtc gggttgggcg ttgacgagga cggtcctgaa tcgtgcgagg
      481 tcttcgcggt cgagatgccc gtggcgctcg acgacctttt cgagcggatg cccccttcgg
      541 gtcacgcagg catagggcgt cgtgaagaga ggcgtcgagt gaatgccggg ctgaacgccc
      601 tggcgcgcat agaaggccat gtcgagtctg tcggccctca ggtcctcgaa catgtgttcg
      661 tcggcctgaa ccgcgcggaa ctgcacgccg gggagcttgg tctgaagctc tccgaacacg
      721 gtagcaaggc ccaccgcgaa ggcgttgtcg taggtggcga tgcgcacgat ctgcttgagg
      781 agcgcgggct gcacctcctc gtcgagcgtg agcgcctcga tctcgcgcac cacgcgctgc
      841 acgcggtcgt agcgccgcgc ggccgcgggc gttgccttca tcagcagccc tgagcgcacg
      901 aagagcggat cggcccaagc tttgcgcagc ctcgcgagct gacggttcgc gctcgagaga
      961 ctgatgttca tctcggccgc cgccttcggg agcgactccg tccgatagag gacgagaaag
     1021 aaggcaagcg tctcgccgtc catctgcagg acgcgccgca tgggaagtgt tgcgggttca
     1081 gacatggtct tgctccgatt tctgacattg aggttcggaa attctcccat tttccgaaac
     1141 gaggcgcaac catagaattt gagtcatccg gagcgcggga cgttctcccg ttttttccgc
     1201 gctcaaaccg attcaatgga attcctgcca tgcaactcaa gtcatccgtc ctttccgctg
     1261 cggtcaagtc cgcactcgtc gccgcaggcc ttgccgccgg cgtcgcttcc gccatgacgc
     1321 ccggcgccta tgaagccacg gcccagggcc gcaatgccga catcaccgtc cgcgtgaccg
     1381 tttccgccga caagattgag aagatcgaaa tcgtgaagca ctccgaaact ccgggcatcg
     1441 gcgacctggc cgtcaacgag ctgcccgcgg ccatgctctc cgcccagacc gccgccgtcg
     1501 acgccgtcgg cggtgccacg atgagttccg cagccatcaa gaaggccgtt gccgaagctc
     1561 tcaggaaggc cggcgcgtcc gagttcctca cggccccgaa ggcgggtgca gacaaggctg
     1621 ccgccgccga cttcaagacg agcgccgacg tgatcgtcgt cggtgcgggc ggcgccggca
     1681 tggttgccgc cgcgacggcc gtcgacaacg gcgcgaccgt gatcgtcctt gaga
//
LOCUS       DBMDTI010000369         1714 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_15126_length_1714_cov_1.927464, whole genome shotgun sequence.
ACCESSION   DBMDTI010000369 DBMDTI010000000
VERSION     DBMDTI010000369.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15126_length_1714_cov_1.927464"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            498..>1714
                     /locus_tag="ACZPB6_10680"
     CDS             498..>1714
                     /locus_tag="ACZPB6_10680"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR1184964.1"
                     /translation="MSSNPQNLADDGLERIKALDVLTTLPELLTHHLTVRPDDVAYKV
                     YDNASKSWKDLTFKNVYDQVHAWRHAFAARSELVRGDRVAMLLPNCPEAVFFDLSALS
                     NTLVPVPLHAIDTPKSSAFILNDSGAKVLVTNKLLKWKQIREAAETPEIRLVVITDDK
                     DCDDLEAPIPVMTKETFLKLGETSQLPKEAPKATDLAALVYTSGTTGNPKGVMLTHRN
                     VISNIQGVLKNLQPSGHETFLSFLPLSHTFERTTSYYLALGLGYTTAFNRSIANLQAD
                     FREIRPTVLMSVPRVYEMIYAKLQDGLAKKSKFVRYLFDWAVEVGWRRFCRENGLPVE
                     SSSRAWLDPFVAGFLDKKVGSQLRAVFGDRIHLYISGGAALSPAVARTFFALGVPIYQ
                     GYGMTETSPIISV"
BASE COUNT          344 a          605 c          433 g          332 t
ORIGIN      
        1 agccccctag cagaggaacg cggagtgtta acgaggcagg accaaccaca tcgtcaatct
       61 gcgtttgagg aggagcaatg aacttttgtt ttcttgcttg gagataaagt tactcgcatt
      121 tacctcaggt tgcaaatatt ctgaaaacgg cttccaaggc ggcttgcagg ctctttaaac
      181 gacggcttca tacgattttt gaaaaaaata tgagcgccac tagtggtgtt caacggcgtc
      241 attttctacg tataaatacg caacccgtat aactttttct tttcccatcc aaatctcccg
      301 tgcatcatcc gcacggatga ccctgaccac aaatgcaggg ggcttgtctt atcattcatt
      361 atttgcgccg gaagcacgct tccggtcccg gatactgaca agacatagtc aggcagccgg
      421 cctccgtccg cccgagcacc ctccgggcgg gatttgagcg gacgacgccc ccccgattcg
      481 aactaggact attaacagtg agctccaatc cccagaacct tgcggacgac ggccttgaac
      541 gcatcaaggc gcttgacgtc cttacgacgc ttcccgaact cttgacgcat cacctgaccg
      601 tcaggcccga cgacgttgcc tacaaggtct acgacaatgc gtccaagtcc tggaaggatc
      661 tcaccttcaa gaacgtctac gatcaggtcc acgcctggcg ccatgccttc gcggcgcgct
      721 ccgaactcgt tcgcggcgac cgcgtggcga tgctcctgcc caactgcccc gaagcggtct
      781 tcttcgacct ctccgcgctc tcgaacacgc tcgtgccggt gccgctgcac gccatcgaca
      841 cgccgaagtc ctccgccttc atcctgaacg actccggtgc caaggtcctc gtgaccaaca
      901 agctcctcaa gtggaagcag atccgcgagg ccgccgagac gcccgagatc cgcctcgtcg
      961 tcatcaccga cgacaaggac tgcgacgatc tggaggcgcc gattcccgtc atgaccaagg
     1021 agaccttcct caagctcggc gaaacgagcc agctgcccaa ggaggctccc aaggccaccg
     1081 acctcgccgc gctcgtctac acctcgggca ccacgggcaa ccccaagggc gtgatgctca
     1141 cgcaccgcaa cgtcatctcg aacatccagg gcgtgctcaa gaacctgcag ccgagcggtc
     1201 acgagacctt cctctcgttc ctgccgctct cgcacacctt cgaacgcacg acctcctact
     1261 acctcgcgct cggtctgggc tacaccaccg ccttcaaccg ctccatcgcg aacctgcagg
     1321 ccgacttccg cgagatccgc cccacggtcc tcatgtccgt gccgcgcgtc tatgaaatga
     1381 tctacgcgaa gctgcaggac ggcctcgcca agaagtccaa gttcgtccgc tacctcttcg
     1441 actgggccgt cgaagtgggc tggcgccgct tctgccgcga gaacggcctg cccgtcgagt
     1501 cgtcctcccg cgcctggctc gaccccttcg tcgcaggctt cctcgacaag aaggtcggca
     1561 gccagctgcg cgccgtcttc ggcgaccgca tccacctcta catctcgggc ggcgcagccc
     1621 tgagccccgc cgtcgcgcgc accttcttcg cgctcggcgt gcccatctat cagggctacg
     1681 gcatgaccga gacctccccg atcatctcgg tgaa
//
LOCUS       DBMDTI010000370         1704 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_15232_length_1704_cov_1.696195, whole genome shotgun sequence.
ACCESSION   DBMDTI010000370 DBMDTI010000000
VERSION     DBMDTI010000370.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15232_length_1704_cov_1.696195"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="ACZPB6_10685"
     CDS             <1..714
                     /locus_tag="ACZPB6_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1184965.1"
                     /translation="YFDGATGDGLDLGAMVGGHTVGMSNIILLPYAGGRLLDYVGGDI
                     YVNSSGERFMNEAMPIYDISEAILAQPGRTCWVITDSRSRKGGTLGLKLADGTVRRSN
                     TIEEMARAMDVPVNTLRRTISEYNEDVAKGLDRRFGKRIFTQAIDSPPYYWGREAVYV
                     HMTLGGLAVSPEARLLDRRGMPIPGFWAAGETTGGIFGRGRPGGMSLITCLVQGRDAG
                     RAAAERSLALKPKAKKVEK"
     gene            complement(744..1241)
                     /locus_tag="ACZPB6_10690"
     CDS             complement(744..1241)
                     /locus_tag="ACZPB6_10690"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR1184966.1"
                     /translation="MDIQKDNRMYMQIALLTSQRSYARRLKVGCVIVKNHSIISFGWN
                     GMPTGYDNCCEMEVDGRLVTRPEVQHAELNAIAKLAENGYSSKGAAIFITHSPCIHCA
                     LLIQKCGITDVYYHTLYRSSEGLEFLKRAGVNVTHLDVEPPFPMQPCDCRIEGCDCGG
                     TGSVH"
     gene            complement(1371..>1704)
                     /locus_tag="ACZPB6_10695"
     CDS             complement(1371..>1704)
                     /locus_tag="ACZPB6_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR1184967.1"
                     /translation="ADPHYVWMDEEYNQYEVEADVMAEALKYLEDGMAAEAVFYEGRA
                     ISIELPTILVREVTYTEPAVKGDTSGKVMKPAKIATGYELSVPAFVEAGDKIEIDTRT
                     GEYRNRVK"
BASE COUNT          299 a          482 c          600 g          323 t
ORIGIN      
        1 tatttcgacg gtgccacggg cgatgggctc gacctgggcg ccatggtggg cggccacacg
       61 gtcgggatga gtaacatcat cctgctgccc tacgcgggcg ggcgtcttct cgactacgtg
      121 ggcggggaca tctatgtcaa cagctcgggc gagcgcttca tgaacgaggc gatgcccatc
      181 tacgacatca gcgaggccat tctggcgcag cccggccgca cgtgttgggt gatcacggac
      241 agccgctcga gaaagggcgg gacgctcggc ctcaagctcg ccgacggcac ggtgcgccgc
      301 tcgaacacga tcgaggagat ggcaagagcc atggatgtgc ccgtcaacac cctcaggcgc
      361 acgatcagcg aatacaacga ggacgtcgcc aagggccttg accgaaggtt cggcaagcgc
      421 atcttcacgc aggcgatcga ttcgccgccc tactactggg gacgggaggc ggtgtacgtg
      481 cacatgacgc tgggcggcct cgcggtgagt cccgaggcgc ggcttctgga ccgcaggggc
      541 atgccgatcc cgggattctg ggcggcgggc gagacgacgg gcgggatctt cggacgcggg
      601 cgtccgggcg gcatgtcgct catcacctgt ctcgtgcagg gacgcgacgc gggccgcgcg
      661 gcggccgaaa ggtcgctcgc gctcaagccg aaggcgaaaa aagtggagaa gtgatccgac
      721 ctgatcgggc cggatgcggg atcctagtgc acggaacccg tgccgccgca gtcgcagccc
      781 tcgatcctgc agtcgcacgg ctgcatcggg aacggcggct cgacgtcaag gtgcgtgacg
      841 ttgacgcccg cgcgcttgag gaattcaagg ccctcgctgc tgcggtagag cgtgtggtag
      901 tagacgtcgg tgatgccgca cttctggatg aggagcgcgc agtggatgca gggactgtgc
      961 gtgatgaaga tggcggcgcc cttgctggag tagccgttct ccgcgagctt cgcgatggcg
     1021 ttgagctcgg cgtgctgcac ttcgggacgg gtcacgagac ggccgtccac ctccatttcg
     1081 cagcagttgt cgtagcccgt gggcataccg ttccagccga acgagatgat ggagtggttc
     1141 ttgacgatca cgcagcccac cttgaggcgg cgggcatagc ttctctggga ggtgagaagc
     1201 gcgatctgca tgtacatgcg gttgtctttc tggatgtcca tggtgctaga gcctgcggag
     1261 tacgttgatg ctgagacgcc tcgcagtgta gcgcaagccg gaacaaaaga aaaacccgac
     1321 gtcctgtgag gagcgtcggg ttttctgttc gatcgaccga tcggtgaaga ttacttcacg
     1381 cggttgcggt attcgcccgt acgggtgtcg atttcgatct tgtcgccggc ttcaacgaag
     1441 gccgggacgg agagttcgta gccggtggcg atcttggcgg gcttcatgac cttgccggag
     1501 gtgtcgccct tgacggccgg ttccgtgtag gtgacttcac ggacgaggat cgtcgggagt
     1561 tcgatggaga tggcgcggcc ttcgtagaag acggcttcag cagccatgcc gtcctcaagg
     1621 tacttgaggg cttcggccat gacgtcggct tcgacttcgt actggttgta ttcttcgtcc
     1681 atccagacgt agtgcggatc ggcg
//
LOCUS       DBMDTI010000371         1692 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_15369_length_1692_cov_2.539283, whole genome shotgun sequence.
ACCESSION   DBMDTI010000371 DBMDTI010000000
VERSION     DBMDTI010000371.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15369_length_1692_cov_2.539283"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="ACZPB6_10700"
     CDS             <1..767
                     /locus_tag="ACZPB6_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184968.1"
                     /translation="TSRRSIFDGIREKMAKRREEKARAKAAVKAAQATQAAEGGEAAD
                     AAFDASAVPEAQKPSFFSGMKDKIVGLRVKQRIADLKVKEKIADLNVKERIENLDVKG
                     RIERLDVKGRIENLNLKDKMDKIKGVDYKKWMPSFGKSAAARAAGAAAAEAAQTAEAA
                     EKAETAEAAQPVQAESAAPAAAPVDVQPEVVQSEAAQSESTVEEERTTEVQAPAVSES
                     SVKDVPASDAPADASAEAASEEAKPALTDDLKKTEQ"
     gene            778..>1692
                     /locus_tag="ACZPB6_10705"
     CDS             778..>1692
                     /locus_tag="ACZPB6_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR1184969.1"
                     /translation="MSRRTRFIDRLTQPAGTLPALVWAACILLILALTVIGFPKGALS
                     TDILDLLPSSSADVRTGELAQAATRGIADRVIWAVREDASNPGAADEFVRSLGERELV
                     TSLSGRMDEADRRIWLEAMRRDVLGLLDEKTVSGLLGDKAKGHLRWMLGQLYSPVAGV
                     TGDEWRADPWLLLRSVMMSQGRGMPTLDDGWITAKDSEGRSWRLLTGRVPESALSGDG
                     LKAWIAAEADIRRAVLSERPDAAIAGQGVLYYSHHAASQAERDMKRLGSLSCVLLAVF
                     IFVAFRSFRPVLLCLLSVSAGALCGTAAT"
BASE COUNT          318 a          560 c          540 g          274 t
ORIGIN      
        1 acacctcccg ccgttcgatc ttcgacggca tcagggagaa gatggcgaag cgccgcgagg
       61 aaaaggcgag ggcgaaggcc gccgtcaagg ccgcccaggc cactcaggct gccgagggtg
      121 gtgaagccgc agacgccgcg ttcgatgcgt ccgccgttcc cgaggctcaa aagccctcct
      181 tcttctccgg catgaaggac aagatcgtcg gccttcgcgt caagcagagg attgcggatc
      241 tcaaggtgaa ggaaaagatc gcggacctca acgtcaagga gaggatcgag aacctcgacg
      301 tcaagggcag gattgaaagg ctcgacgtca agggccgcat cgaaaatctc aacctcaagg
      361 acaagatgga caagatcaag ggcgtcgact acaagaagtg gatgccgtcc ttcggcaagt
      421 ccgccgctgc cagagccgca ggcgccgctg ccgctgaagc cgctcaaact gcagaagctg
      481 ctgaaaaggc tgagactgcc gaggccgctc agcccgttca ggccgaatcc gccgcgcccg
      541 ctgcagcgcc tgtcgacgtg cagcccgaag tcgtgcagtc tgaagccgct cagtccgaga
      601 gcaccgtcga agaggaacgc accaccgaag ttcaggcacc cgccgtgagc gaatcttccg
      661 tgaaggacgt tcccgcgtcc gatgcgccgg ctgatgcatc tgccgaggcg gcgtccgaag
      721 aggcgaagcc cgcgctcacg gacgacctga agaagaccga acaataacca gaggatcatg
      781 agcaggagga cgcggttcat cgacaggctc acgcaacccg caggcacctt gcctgcgctg
      841 gtttgggccg cgtgcatcct tctcattctt gcgctcaccg tcatcggctt tccgaagggc
      901 gccctttcga ccgacattct tgatctgctg ccgtcctctt ccgccgacgt gaggacggga
      961 gaacttgctc aggccgccac gcgcggcatc gccgaccgcg tgatctgggc cgtccgcgag
     1021 gacgcgtcga accccggcgc tgcggacgaa ttcgtccgaa gcctcgggga gcgcgagctc
     1081 gttacgtcgc tttcgggccg catggacgaa gcggaccgca ggatctggct tgaggccatg
     1141 aggcgggacg tactcggcct gctcgacgag aagaccgtct cgggacttct cggggacaag
     1201 gcaaaggggc acctgaggtg gatgctcggc cagctctaca gtcccgtcgc aggcgtcacc
     1261 ggcgacgagt ggcgcgcgga tccctggctt cttctccgct cggtcatgat gtctcaggga
     1321 agaggcatgc cgacgctcga cgacggctgg atcaccgcaa aggattccga agggcgctcc
     1381 tggcgcctgc tgacgggccg cgtgcccgaa tccgcgctct cgggcgacgg gctcaaggcc
     1441 tggatcgccg ccgaggccga catccgcagg gccgttctgt ccgaacgtcc cgacgccgcc
     1501 atcgcagggc agggcgttct ctactacagc catcacgcgg cttctcaggc cgagcgcgac
     1561 atgaagcgtc tcggttcgct ctcctgcgtg ctcctcgccg tcttcatctt cgtcgccttc
     1621 cgaagcttca ggcccgttct cctctgcctg ctttccgtct cggcgggcgc gctctgcggc
     1681 acggccgcca cg
//
LOCUS       DBMDTI010000372         1683 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_15486_length_1683_cov_1.806574, whole genome shotgun sequence.
ACCESSION   DBMDTI010000372 DBMDTI010000000
VERSION     DBMDTI010000372.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15486_length_1683_cov_1.806574"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="ACZPB6_10710"
     CDS             complement(<1..235)
                     /locus_tag="ACZPB6_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012698.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1184970.1"
                     /translation="MRTAEHDLFWRGRSVPLTEQEFRILSALAKHPGAVIRYAELLRQ
                     GWGDGEVDVARLRVAVARIRKKLSANGINPMIIS"
     gene            complement(319..726)
                     /locus_tag="ACZPB6_10715"
                     /pseudo
     CDS             complement(319..726)
                     /locus_tag="ACZPB6_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(751..>1683)
                     /locus_tag="ACZPB6_10720"
     CDS             complement(751..>1683)
                     /locus_tag="ACZPB6_10720"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1184971.1"
                     /translation="DEDWRLRYGNHAAGTLIGVDGMARLAEVCRSGLERRSDGTALVT
                     IEAGGRSLVLECDAFPFGAAADGLRSGCCVLFRDVTGRTEVERMKDDLISIVAHELRT
                     PLTACRLQLDLLVKENGPQSAYAAMGEDLDHLGRIVDDWLSVARIDGGSYRVESRIVQ
                     TMPLVTRAVRLVRSRHEFRFDYDIAEEAECIRVDPSAFVELLVNLFTNACRHARPGEE
                     PRIEFRARVDEMLVVDVCDSGTGFPEGEAEKLFDRFYQVEQGNKRRTGGTGLGLVICR
                     AICRAHGGRIEALRLEGRTVFRITLPMPSQTDGL"
BASE COUNT          268 a          540 c          584 g          291 t
ORIGIN      
        1 aggagatgat catgggattg atcccgttgg cggagagctt cttgcggatg cgcgcaaccg
       61 ccaccctgag gcgcgcgacg tcaacctcgc cgtcgcccca gccctggcgg agcagctccg
      121 catagcggat gacggcaccg gggtgcttcg cgagggccga gaggatcctg aactcctgct
      181 ccgtgagcgg gacggagcgc ccgcgccaga agaggtcgtg ctcggcggtc cgcagttcaa
      241 gcgggccgtt tgcgacgacc tcgagacccg catgtcggag cctgaggcgc gtcgaagcag
      301 cgctctgatg cgcacctcga gctccgcgaa cgcaaagggc ttgacgaggt agtcgtccgc
      361 ccccgtgttg aagctcttcg tcttgtcctg caggaggtcg cgcgccgtga ggatcatgac
      421 gggcaccttt gagaaggtcc tcagttcggc gagcgtctcg tgtccgtcct tgacgggcat
      481 catcaggtcg agcacgacga gatcgggctc gacgggcgag cgcctgaaga cctcgagggc
      541 ctcctcgccg tcctgcgcct gacagacgtc aaagcccgac tgctcgagga attcggccgt
      601 caggcgcctg attcgcgttt cgtcgtcggc gatgagaatg agaggtcgtg tggtcatgtc
      661 tgtcgggagc ctccggcccg tactgcgcac cgtcgtcgta tccgtccgca ggcctgcggt
      721 gcgcatggag gcctgcggga aaggaatgcg tcagagcccg tcggtctgcg agggcatcgg
      781 gagcgtgatc ctgaagacgg tccgtccctc aagtctgagc gcctcgatgc gtccgccgtg
      841 ggcgcggcag atcgcgcggc agatgacgag gccgagcccc gtgccgcccg tgcggcgctt
      901 gttgccctgc tcgacctgat agaagcggtc gaagagcttt tcggcctcgc cctcggggaa
      961 gcccgtgccc gaatcgcaga cgtccacgac gagcatttcg tcaacgcgtg cgcggaattc
     1021 gatgcggggt tcctcgcccg gccttgcgtg gcggcaggcg ttggtgaaga ggttgacgag
     1081 cagctcgacg aaggccgacg gatcgacgcg gatgcactcg gcctcctcgg cgatgtcgta
     1141 gtcgaagcgg aattcgtggc ggcttctcac gagtctcacg gcgcgggtga cgagcggcat
     1201 ggtctgcacg atgcgcgact cgaccctgta ggagccgccg tcgattctgg ccacggagag
     1261 ccagtcgtcg acgattctgc cgagatgatc gaggtcctcg cccatggccg cataggcgga
     1321 ctgcggaccg ttctccttga cgagaaggtc gagctgcagg cgacaggcgg tgagcggggt
     1381 gcgcagttcg tgcgcgacga tgctgatgag gtcgtccttc atgcgctcga cttcggtcct
     1441 gcccgtcacg tccctgaaga gcacgcagca gccactgcgc agaccgtccg cagccgcgcc
     1501 gaacgggaag gcgtcgcact cgagcaccag gcttcgtccg cccgcctcga tcgtgacgag
     1561 ggccgtgccg tcgcttctgc gctcgagtcc gctgcggcag acttcggcca gacgcgccat
     1621 gccgtcaacg ccgatcaggg tgcccgccgc atgattgccg tagcgaagcc gccagtcctc
     1681 gtc
//
LOCUS       DBMDTI010000373         1681 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_15507_length_1681_cov_1.488608, whole genome shotgun sequence.
ACCESSION   DBMDTI010000373 DBMDTI010000000
VERSION     DBMDTI010000373.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15507_length_1681_cov_1.488608"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(234..1586)
                     /locus_tag="ACZPB6_10725"
     CDS             complement(234..1586)
                     /locus_tag="ACZPB6_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184972.1"
                     /translation="MDSEILTWLLIAACAAGFGGGAAWQAMRRRAPARTRRNVDKVAA
                     RPVKASPAPKAPAPAPEVRKPQHGVHRPSMLALTTLENSVTAADLFIQRALELDEPYY
                     RELPAEAEQLKALKGLFARCRTLDIVPGDGLSKDVFSLNFSTMIVEQLRSGFMPHPNA
                     TSNDLQIIALANGCEPLGDPMAVEDFGVGGFPHVTQLWMMVDPTDKKHELDDLLEYEL
                     NGIRNLLPRVKLLVPALDAMKWGDYWDKLFDLVRDVRRLGAGEGQALMRTERTDVLAK
                     DMYAINRRIDESLATLEKNMKTPEEADVALTSSIPHMLERELSVLFLRTLAVIRVISG
                     DNYIHGMHCSAHIRRNVEHFPDVHSLLDKARHIVYDALEVQGRGMNAPAMDIAGQVKR
                     DADELGRAHDESVERLKADVERLQAAIDRYLILQGRPRRYAVRLNERNEVLALLVLEH
                     "
BASE COUNT          273 a          480 c          620 g          308 t
ORIGIN      
        1 cgaaggcata ttttcgtacg caggccggag gtatcggcgg tcgtccctga aggcgaggat
       61 ttcggccgcg gtggcctcgg aaatgcccct gagaggcacg gtttcggatg tttcggatgt
      121 ttcggcggtt tcggcctgca tagcgatgct ccctgaagaa aaacggaaga actcaagaca
      181 aaatgaaagc ccggaggctg agcgccttcg ggcttttgag tctcaagcga ggatcagtgc
      241 tcgagcacga gaagggccaa gacctcgttc ctttcgttga gcctcacggc atagcggcgc
      301 gggcggccct gaaggatcag gtagcggtcg atcgcggcct gcaggcgctc gacgtcggcc
      361 ttgaggcgct cgacggactc gtcgtgggcg cggccgagct cgtcggcgtc gcgcttgacc
      421 tggcccgcga tgtccatggc gggggcgttc atgccgcggc cctggacctc gagcgcgtcg
      481 tagacgatgt ggcgcgcctt gtcgaggagc gagtggacgt cggggaagtg ctcgacgttg
      541 cggcggatgt gcgcggagca gtgcatgccg tggatgtagt tgtcgcccga gatcacgcgg
      601 atgacggcga gcgtgcgcag gaagagcacg gagagctcgc gctcgagcat gtgcggaatg
      661 ctgctcgtga gcgccacgtc ggcctcctcg ggcgtcttca tgttcttttc aagggtggcg
      721 aggctctcgt cgatgcggcg gttgatggcg tacatgtcct tcgcgaggac gtcggtgcgc
      781 tccgtgcgca tgagggcctg gccctcgccc gcgccgaggc ggcggacgtc gcgcaccagg
      841 tcgaagagct tgtcccagta gtcgccccac ttcatcgcgt cgagcgcggg cacaagaagc
      901 ttcacgcgcg gcagaagatt gcggatgccg ttgagctcgt actcgagcag gtcgtcgagc
      961 tcgtgcttct tgtcggtcgg gtcgaccatc atccagagct gcgtgacgtg cgggaagccg
     1021 cccacgccga agtcctcgac cgccatcgga tcaccgagcg gctcgcagcc gttggcgagc
     1081 gcgatgatct gcaggtcgtt ggaggtggcg ttcggatgcg gcatgaagcc cgagcggagc
     1141 tgctcgacga tcatcgtcga gaagttgagg gagaagacgt ccttcgagag gccgtcgccc
     1201 ggcacgatgt cgagcgtgcg gcagcgggca aagaggccct tgagggcctt gagctgctcg
     1261 gcttcggcgg gaagctccct gtagtagggc tcgtccagct cgagggctcg ctgaatgaag
     1321 aggtcggccg cggtcacgct gttctcaagc gtcgtgagcg cgagcatcga tggacggtga
     1381 acgccgtgct ggggcttgcg cacctcgggg gcgggagcgg gcgccttggg cgcgggcgag
     1441 gccttgacgg ggcgggcggc caccttgtcg acgttcctgc gggtgcgcgc gggcgcgcgg
     1501 cggcgcatgg cctgccaggc tgcaccgccg ccgaagcctg cggcgcaagc cgcgatcaaa
     1561 agccaggtga ggatttcgct gtccatagaa ttgccgcgag ggaacatccg acttcagtcg
     1621 ggtgaggaat cgcggcttac tcctttcgta gttcagtcaa aaaagttttg cgtgtttcga
     1681 g
//
LOCUS       DBMDTI010000374         1677 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_15557_length_1677_cov_2.680203, whole genome shotgun sequence.
ACCESSION   DBMDTI010000374 DBMDTI010000000
VERSION     DBMDTI010000374.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15557_length_1677_cov_2.680203"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            221..1141
                     /locus_tag="ACZPB6_10730"
     CDS             221..1141
                     /locus_tag="ACZPB6_10730"
                     /inference="COORDINATES: protein motif:HMM:NF018431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1184973.1"
                     /translation="MLERLQNLVLEALPEEGRIENPMPCLALTRFNGPQQSRQCFYHP
                     MMTLGLGGEKESLIGGRPVVYGAGEAVVVALDLPGAYQIRNASPEQPFLSLSIRLDRA
                     IITELLTEAPELRPAPNGRDAQESVSVEHLPDEILNALVRYLEAMRSPRRAAVLGPMI
                     LKEIHYLLLEGPQGRVLADVCSEAAPGSRILTAVRWLREHFDEPLDIATISERTAMAP
                     STFHRQFRAVTSLSPVQYQKRLRLHEAQRLMLVESLGVEAAARRVGYESCSQFSREYK
                     RLFGDAPARDIREKSVAASDAGKCASIMEA"
     gene            1258..>1677
                     /locus_tag="ACZPB6_10735"
     CDS             1258..>1677
                     /locus_tag="ACZPB6_10735"
                     /inference="COORDINATES: protein motif:HMM:NF024094.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIR1184974.1"
                     /translation="MEKHHAKAPSSRLRRPCHHRPRLRRRIAVVYFTKTGNTKSLAEA
                     VRHMTGAALFRVETVEPYPESYGSATEIVKDELQRGVIRPIRPLAFNPDDYDVVVLAT
                     PTWWHHAAMPLQTWIRSVDLSAKKILTANTHGGGGLMH"
BASE COUNT          327 a          589 c          488 g          273 t
ORIGIN      
        1 ccccaaaaac cgaaaactca cggaaaaacc gaacggcctg cctcagctgt tcggacgatc
       61 ggaagaagga cttaagaacg gacttctcgc cccgccgaat aagggcatcg gacagggctg
      121 cgagaggatc gtgcaggcgg cacgcttgac gagcccctgc aggaaaggca gagtattgga
      181 catgattcca tgctcaagtt ctgtacgaag atccgtccaa atgctcgaac gccttcaaaa
      241 tctcgtcctc gaggccctgc ctgaagaggg ccgcatcgaa aacccgatgc cctgtctggc
      301 cctcacccgc ttcaacgggc cgcagcaaag ccgccagtgt ttctaccatc caatgatgac
      361 gctagggctc ggcggcgaga aggaaagcct catcggcgga cgccccgtcg tctacggcgc
      421 gggagaggcg gtcgtcgtgg cgctcgacct tcccggcgcc tatcagatcc ggaacgcctc
      481 gcccgagcaa cccttcctgt cgctctccat tcgtcttgac cgtgcgatca tcacggagct
      541 cctcaccgag gcgcccgaac tcaggcccgc cccgaacggc cgcgacgcac aggagagcgt
      601 gagtgtggag cacctgcccg acgaaattct caatgcgctc gtgcgctacc ttgaggccat
      661 gcgaagcccg cgccgtgcgg cggttctcgg gcccatgatc ctcaaggaga tccactatct
      721 gcttctcgaa ggccctcagg gccgggttct agccgacgtc tgctcggaag ccgcgccggg
      781 atcccgcatc ctcacggccg ttcgctggct tcgcgagcac ttcgacgagc cgctcgacat
      841 cgcgacgatt tcggagcgca ccgccatggc gccgtcgacc ttccaccgcc agttcagggc
      901 cgtcacctcg ctctcgcccg tgcagtacca gaagcgcctg cgccttcacg aggcccagcg
      961 ccttatgctc gtcgagtccc tcggcgtcga ggccgcggca agacgcgtgg gctacgaaag
     1021 ctgctcgcag ttctcccgcg aatacaagcg cctcttcgga gacgcgcccg ccagggacat
     1081 cagggagaag tcggtcgccg cctccgacgc agggaaatgc gcctccataa tggaggcctg
     1141 aggcacgcct ctcaggattg agaggatcgg gcaggaactt gagacatctc gacatcgtcc
     1201 cgagggcgcg tttttctacc atgactgcaa cgacggagct caggctccgc cggcctcatg
     1261 gagaaacatc atgcaaaagc gccgtcttct cgcctccgcc gcccttgcca tcaccgccct
     1321 cggctgcggc gccgcatcgc cgtcgtctac ttcacgaaga cgggcaacac gaaaagtctc
     1381 gccgaggcgg tccgacacat gacgggcgcg gcgctctttc gcgtcgagac cgtcgagccc
     1441 tatccggaaa gctatggttc cgccaccgag atcgtgaagg acgagctcca gcgcggcgtc
     1501 atccgtccga tcaggccgct cgccttcaat cccgacgact acgacgtagt cgtgctcgcc
     1561 acgcccacgt ggtggcacca cgcggcgatg ccgctgcaga cctggatcag gtccgtcgac
     1621 ctcagcgcca agaagatcct caccgccaac acccacgggg gcggcggcct catgcac
//
LOCUS       DBMDTI010000375         1675 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_15581_length_1675_cov_1.725540, whole genome shotgun sequence.
ACCESSION   DBMDTI010000375 DBMDTI010000000
VERSION     DBMDTI010000375.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15581_length_1675_cov_1.725540"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(144..473)
                     /locus_tag="ACZPB6_10740"
     CDS             complement(144..473)
                     /locus_tag="ACZPB6_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184975.1"
                     /translation="MMRKVLLSIPPALSTLLFCAHLLYHGVPLAAAAAPLGALILLAV
                     KGAGATRLLALLHVLFACEWARAGGKIVMRRLETGGSIHPALEIMAGVTLFTILAAWC
                     SLARHRD"
     gene            complement(470..871)
                     /locus_tag="ACZPB6_10745"
     CDS             complement(470..871)
                     /locus_tag="ACZPB6_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HIR1184976.1"
                     /translation="MTEANSRMARLETDPVLREEAETIRVMTAMYCRDRHGTSGRPDG
                     LCPECSAFLAYALKRLACCPYGASKPVCAKCRIHCYRAKEKAQAREVMRHAGPRLAAS
                     HPVLSIRHLVKSLTVKAPEKPRNTAAKKEMA"
     gene            complement(979..1566)
                     /locus_tag="ACZPB6_10750"
     CDS             complement(979..1566)
                     /locus_tag="ACZPB6_10750"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR1184977.1"
                     /translation="MAVIVTRDIETPLGKMRVGACEGALVFALWLTGEPERVERIIAR
                     TAGRCGAGAVLQGNSSLIDEAEVQIMEYLAGRRTIFELPIRLHGTDFQRHVWHVLTDI
                     NYGCATTYGMLALMAGCEGGSRAVGSAVGANPLHVIIPCHRVVEKSGRIGGYAGGGAV
                     KFELLAREGKVVSVNAVPGRGFTRLIADAIPKVMG"
BASE COUNT          315 a          557 c          504 g          299 t
ORIGIN      
        1 cctttgatct cggcgatgtc aagggcgtca tgaagtggct cgacgaggcc gacgccgtca
       61 ggagctgaga ctttccctgg cgactgtccg acctgctcgt caaaaaaggc ggtgcatctt
      121 cctgcaccgc ctttttcaac tcctcaatcc cgatgccttg cgagcgagca ccaggccgcg
      181 agaatcgtga agagcgtcac gcccgccatg atctcaagcg caggatgaat cgaaccgccc
      241 gtctcgagcc ttcgcatgac gatcttcccg ccggctctcg cccattcgca ggcaaagagc
      301 acgtgaagaa gtgcgagaag gcgcgtggcg cccgcaccct tcacggccag aaggatgagt
      361 gcgccaagcg gcgcggccgc agccgcaagc ggcacgccgt ggtagagaag atgcgcgcag
      421 aagagcagcg tcgagagcgc gggcgggatc gagagcagca ccttcctcat catgccatct
      481 ccttcttcgc cgcagtgttc ctcggctttt cgggcgcctt caccgtgagg ctcttcacaa
      541 gatgcctgat ggaaagcacg ggatgcgatg ccgcaagacg cggccccgca tggcgcatga
      601 cctcacgcgc ctgcgccttc tcctttgcgc gataacaatg gatgcggcac ttggcgcaga
      661 cgggcttcga cgcaccgtac ggacagcacg caaggcgctt gagcgcatac gcgaggaagg
      721 cgctgcattc gggacagagc ccgtcggggc gccccgacgt gccgtggcgg tccctgcaat
      781 acatcgccgt catgactcgg atcgtctccg cctcctcgcg aaggacggga tcggtctcaa
      841 gtcttgccat gcgagaattc gcctcagtca tgtgcatctc tcctgatcgc ctgtgaaaaa
      901 aggggatctt agtgaacaca gtctgaggct agccttaaga acggcaccat cccctccttc
      961 ggagagaggc ggcgggcgtc accccatcac cttcgggatg gcgtcggcga tgagtctcgt
     1021 gaaaccccgc cccggcacgg cattgacgct caccaccttt ccctctcgcg cgaggagctc
     1081 gaacttcacc gccccgccgc ccgcatagcc gcctatcctg ccggacttct cgaccacgcg
     1141 gtggcatgga atgatgacgt gcaggggatt ggcgccgacg gccgagccga cggcgcgcga
     1201 gccaccctcg catcccgcca tgagggcgag catgccgtag gtggtggcgc agccgtagtt
     1261 gatgtcggtc agcacgtgcc agacgtgccg ctgaaagtcg gtgccgtgaa gacgaatggg
     1321 aagctcgaag atcgtgcgcc ggcccgcgag gtactccatg atctgcacct cggcctcgtc
     1381 gatgagggac gagttgccct gcaagaccgc cccggcgccg catcgccctg ccgtacgggc
     1441 gatgatgcgc tccactcttt cgggctctcc cgtgagccag agcgcaaaca ccagcgcgcc
     1501 ttcgcaggct cccactctca ttttgccgag cggcgtttct atgtctcggg taacgatgac
     1561 ggccatgatc ttctcctgca agcaaaagtc tgaagagatt agcttagcgc agatcctctc
     1621 cgcgctcctc ccctgcactt tcgccgcacg ggacgatcat gagcgctgcc agtat
//
LOCUS       DBMDTI010000376         1655 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_15858_length_1655_cov_2.375804, whole genome shotgun sequence.
ACCESSION   DBMDTI010000376 DBMDTI010000000
VERSION     DBMDTI010000376.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15858_length_1655_cov_2.375804"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="ACZPB6_10755"
     CDS             <1..558
                     /locus_tag="ACZPB6_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR1184978.1"
                     /translation="ASERDLISAEANIGVARAAFFPSVSITAGAGTTGMQLSNLFDAH
                     TGFWSFTPSVNLPIFTGGRNVATLEAAKVGQKIAVANYEKAIQTAFREVSDALATEGT
                     MDRELTARREYAEAATKAWELSDASYKAGAVSYNDVLTSQRAMVGAKQALIGAELSKS
                     MSAITLYKVLGGGTTVAEAQPADKE"
     gene            562..1284
                     /locus_tag="ACZPB6_10760"
     CDS             562..1284
                     /locus_tag="ACZPB6_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1184979.1"
                     /translation="MAKVTFTIHKAGKHQRHSRRNQILDAAQRCFKKQGFHKTTLRDI
                     AQEFGMSAGHIYNYFSNKEAIIEALVELRTQEFLDMIDTDKFADLPPEERMDKELGQV
                     VDAYLNLDNTSLSIAIMNEALINPKVYEIVVAAASKVRNHIVEAQLLAMKEERRYAPP
                     YEVIESRCMTIRAMLEGLRLAHIFTPEMDVPLVREMAIKRIKQIIALDEAEDQAAAAA
                     EAKLKAEAMACAYYPVSENPEA"
BASE COUNT          367 a          572 c          449 g          267 t
ORIGIN      
        1 gcgagcgaac gtgacctgat cagcgccgaa gccaacatcg gcgtcgcccg agccgccttc
       61 ttcccgagcg tctcgatcac cgccggcgca ggcaccacgg gcatgcagct ctcgaacctc
      121 ttcgacgcgc acacgggctt ctggtccttc acgccctccg tgaaccttcc gatcttcacg
      181 ggcggccgca acgtcgccac ccttgaagcc gccaaggtcg gccagaagat cgccgtcgcg
      241 aactacgaaa aggccattca gaccgccttc agggaagtct ccgacgccct cgccaccgaa
      301 ggcacgatgg accgtgaact cacggcccgc cgcgaatacg ccgaggccgc caccaaggcc
      361 tgggaactct ccgacgccag ctacaaggcg ggcgccgtct cctacaacga cgttctgacg
      421 agccagcgcg ccatggtcgg cgccaagcag gccctcatcg gcgccgaact ctccaagtcg
      481 atgagcgcca tcaccctcta caaggtgctc ggcggcggca cgaccgtagc cgaagcgcag
      541 ccggccgaca aggagtaatc catggccaaa gtgacattca ccattcacaa ggcgggcaag
      601 caccagcgcc actcacgccg caaccagata ctcgacgccg cccagcgctg cttcaagaag
      661 cagggcttcc acaagacgac cctgcgcgac atcgcgcagg agttcgggat gagcgccggc
      721 cacatctaca actacttcag caacaaggaa gcgatcatcg aggcgcttgt cgaactgcgc
      781 acgcaggagt tcctcgacat gatcgacacc gacaagttcg ccgacctgcc gcccgaagag
      841 cgcatggaca aggagctcgg ccaggtagtc gacgcctacc tcaacctcga caacacgtcg
      901 ctctccatcg cgatcatgaa cgaggccctc atcaacccga aggtctatga aatcgtcgtg
      961 gccgccgcga gcaaggtccg caaccacatc gtcgaggctc agttgcttgc gatgaaggag
     1021 gagcgccgct acgcaccgcc ctacgaagtg atcgagagcc gctgcatgac catccgcgcc
     1081 atgctcgaag gcctgcgcct cgcgcacatc ttcacgcccg aaatggacgt gccgctcgtg
     1141 cgtgagatgg ccatcaagcg catcaagcag atcatcgcgc tcgatgaggc cgaggaccaa
     1201 gccgcagcag ccgccgaggc caagctcaag gccgaagcca tggcctgtgc ctactatccg
     1261 gtctccgaga atcctgaggc ctgaggcctg aagcctgaag cctgaagctc caaagtgaaa
     1321 tcgggagtct ccgcaacgga gactcccgat ttttttcatt ctcattttca cgcgtcctcc
     1381 agtcttcccg agagccgcat gtgaatcctc cccgcccaat tattaattgg acggggcttc
     1441 cctgcctttt accagcaagg actgcctggg cacagatagc gggcaaactc agaaatctgc
     1501 gctcacatgg agcgcagctc cgcgttaacc cgaattagag tgtccagggt gcgtgtgatc
     1561 ctcttggcac atgacggaac tttagccgca atgcaccgcc atctcgttgc cgaaatggac
     1621 ttctcgtact ccctgaggaa gtaccgagct ccgat
//
LOCUS       DBMDTI010000377         1650 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_15934_length_1650_cov_2.252421, whole genome shotgun sequence.
ACCESSION   DBMDTI010000377 DBMDTI010000000
VERSION     DBMDTI010000377.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15934_length_1650_cov_2.252421"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="ACZPB6_10765"
     CDS             <1..380
                     /locus_tag="ACZPB6_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR1184980.1"
                     /translation="TLGGGWFLCLAGYMNIWPLFGSANQLLGALVLTALAVFLQSTGR
                     KGWMLWVPMTVMFVVTMTALVQALLKIAAAWSAGTFVFMTHGLQAIIAIALIVLALLV
                     VYHCVGRLFKGGKQDATGAANAA"
     gene            complement(494..817)
                     /locus_tag="ACZPB6_10770"
     CDS             complement(494..817)
                     /locus_tag="ACZPB6_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1184981.1"
                     /translation="MSDTTECRMVTGGEIADELSDAFPDIPTSKARDAFRTILHAMQN
                     ALIRGDRIEIRGFGSFAPRYRQGGMRRNPRSGEKVYAPARWVVIFRAGKDLREAIDAR
                     TERKA"
     gene            957..1033
                     /locus_tag="ACZPB6_10775"
     tRNA            957..1033
                     /locus_tag="ACZPB6_10775"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:991..993,aa:Ile,seq:cat)
     gene            complement(1095..>1650)
                     /locus_tag="ACZPB6_10780"
     CDS             complement(1095..>1650)
                     /locus_tag="ACZPB6_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1184982.1"
                     /translation="VTAFTGIATAFLLPVFLSGSAVPEGEMWLLGDFFGLMSGFCWGL
                     TTISMRATTMNEAPASQMLFCQLASGFAVLLPCAILFGQTHIGLTPLMLSSMGFQTFV
                     VAFASYLVWCQLLKQYLAARLGILVFMTPLFGVLFSVFILGDRIGLPFVAGSLLVLAG
                     LFMVQTKHIGLVRRRRRMSAQADK"
BASE COUNT          300 a          548 c          511 g          291 t
ORIGIN      
        1 tcacgctggg cggcggctgg ttcctctgcc tcgccggcta catgaacatc tggccgctct
       61 tcggctccgc caaccagctc ctcggcgccc tcgtcctcac cgcgctcgcc gtgttcctcc
      121 agagcacggg ccgcaagggc tggatgctgt gggttccgat gaccgtcatg ttcgtcgtca
      181 cgatgacggc cctcgtccaa gcgctcctca agatcgccgc cgcttggagc gcaggcacct
      241 tcgtcttcat gacgcacggt ctgcaggcca tcatcgccat cgcgctcatc gtgctcgcgc
      301 tgctcgtcgt ctaccactgc gtcggccgcc tcttcaaggg cggcaagcag gacgcaaccg
      361 gcgccgccaa cgcagcgtaa tgcaccgaac ctccggtccc gagcacacgg gaccggttcc
      421 cggataagcc gaagcccccg caccgcaagg tgcaggggct tcgtttcgtt tgattcggat
      481 cgtcgggacg caatcaggcc tttcgttccg tgcgcgcgtc gatcgcctcg cgcaggtcct
      541 tgccggccct gaagatcacg acccagcgtg cgggggcgta gaccttctcg cccgagcgcg
      601 gattgcggcg catgccgccc tgacggtagc gaggtgcgaa ggagccgaag cccctgatct
      661 cgatgcggtc gccgcgaatc agcgcgttct gcatcgcatg gaggatggtg cggaacgcgt
      721 cgcgcgcctt gctcgtcgga atgtccggaa acgcgtccga gagttcgtct gcgatttcgc
      781 cgcccgtcac catgcggcat tcggtagtgt cgctcatgtc tcaagcctgt atgtggaaag
      841 gtccccgtat gatatcccat cccgtcgcaa aacgccttcc cgccggtgcg cgacgccccc
      901 aggacttgag acggcatttg atcttgccga ggcctcatga tacaattcgc gctcctgcgc
      961 ccttagctca gtcggttaga gcaggcgact cataatcgct tggtcgctgg ttcaagtcca
     1021 gcagggcgca ccaagacaag aaaggcggct tctcacaaag ccgccttttt tcgttttccg
     1081 tcggatttcc tctgctattt gtccgcctgc gcggacatgc gccttctgcg gcgcacgagg
     1141 ccaatgtgct ttgtctgaac catgaagagg cccgcgagca cgaggagcga gcccgccacg
     1201 aacggcagtc cgatcctgtc gccgaggatg aagaccgaga agagcacgcc gaagagcggc
     1261 gtcatgaaga cgaggatccc gagccttgcc gccagatact gcttgaggag ctgacaccag
     1321 acgaggtagc tcgcgaacgc cacgacgaag gtctggaagc ccatggacga gagcatgagg
     1381 ggcgtgagcc cgatgtgcgt ctgtccgaag aggatcgcgc agggcaggag caccgcgaag
     1441 cccgaggcga gctggcagaa gagcatctgc gaggcgggcg cctcgttcat cgtcgtggcg
     1501 cgcatcgaga tcgtcgtgag cccccagcag aagcccgaca tcaggccgaa gaagtcgccg
     1561 agcagccaca tctcgccctc gggcaccgca ctccccgaaa gaaacacggg cagcaggaac
     1621 gcggtcgcaa tgcctgtgaa ggccgtcacg
//
LOCUS       DBMDTI010000378         1628 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_16221_length_1628_cov_2.361493, whole genome shotgun sequence.
ACCESSION   DBMDTI010000378 DBMDTI010000000
VERSION     DBMDTI010000378.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16221_length_1628_cov_2.361493"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            122..613
                     /locus_tag="ACZPB6_10785"
     CDS             122..613
                     /locus_tag="ACZPB6_10785"
                     /inference="COORDINATES: protein motif:HMM:NF036859.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1184983.1"
                     /translation="MTDIFRRRLFGAAAATGLAAVPAHAARVDADEELLKKDYPAGRR
                     SAALGQPAPMPEPEARLSGTYDLGASTLNEGEVINASRWGVVYAHVQGGKMTHLRPFE
                     FDYAPSPNLISAIFRTRRLASVSRWFAKASSRTARRAVAAAAKRSSCPSPGKRHSTSW
                     QAK"
     gene            565..1628
                     /locus_tag="ACZPB6_10790"
                     /pseudo
     CDS             565..1628
                     /locus_tag="ACZPB6_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT          329 a          532 c          488 g          279 t
ORIGIN      
        1 ggtaaaaaaa acagcacctc ctggcgtcgt taagaaaaac tgaacggaac atttttgttc
       61 catccggctt ggtaacaggg gtttttgaag agcttcctta gaaaagaaga aggagtcaga
      121 catgacagac atcttccgcc gcaggctctt tggcgcagca gcagccacgg gcctcgccgc
      181 cgttcccgca catgccgccc gcgttgacgc ggacgaagag cttctgaaga aggactatcc
      241 cgccggccgc aggagcgcag ccctcggcca gcccgctccg atgcccgaac ccgaagcgag
      301 gctctccggc acgtacgacc tcggtgcgtc cacgctcaac gaaggcgaag tgatcaacgc
      361 ctcccgctgg ggcgtcgtct acgcgcatgt tcagggcggc aagatgacgc atctgcgtcc
      421 gttcgagttc gactatgcgc cgagtcccaa tctcatctct gccatcttcc gtactcgccg
      481 tctcgcatcc gtcagccgat ggttcgcgaa ggctagctca agaacggccc gaagagccgt
      541 cgcagccgcg gcgaagagaa gttcgtgccc gtctcctggg aaaaggcact cgacctcgtg
      601 gcaggcgaaa tgaagcgcgt ctacaaggac tacggtccga gcgccgtctt cacggctgga
      661 tgagcacggg ccgcgtcaac gccgccatca acctgcagca gcgtctcctc aacctctgcg
      721 gcggcttcat ccagtatgag aactcctact cgacggccgc catcagcaag attcttccgt
      781 acgtcgtcgg cacgggcgat ccgcgctcca cgtcctggga cgtcgtgatc gggcacagcg
      841 agcgcgtggt cttctggggt tgcgacccgc tggtgacgaa cgacatcgac tggcacacga
      901 cgattcacaa tgctgcgggc tacattcgtg cgctgcgcga caagggcgtc acgaccatct
      961 cgatcaatcc cgtccagacc gacacggccg aatacctcgg aagcgaatgg atcgcgccgc
     1021 gtcccggcac ggactgcgcc atgatgctcg gcatgatgca cgagctcgaa gccacgggca
     1081 aggccgacca cgccttccta gagaagtgct gcagcggctg taaggagctt cgcgcctaca
     1141 tcatgagcga tgaggacggc gtcgccaaga cgcccgaatg ggccgccgaa aagacaggcg
     1201 tgcccgccga tcgcatccgc aagcttgcgc acgacctgca tgagcaccgc acgatgctca
     1261 tggtcggctg gggcatccag cgcattcagt acggcgaaca gcctcactgg atggccttcg
     1321 cgctcgcgag cgttctgggc cagatcggcc tgccgggcgg cggcatcggc acgaactacc
     1381 agtattcgag cggcggcgct ccgttggcaa agggcccgtt ccttgcgggc gttcccgcca
     1441 aggtgaagcc ggcgcttccg ttcaacgata actggaaggg ctcgaccacg attccggtcg
     1501 cgcgcttcgt cgaatgcttc ctcaatccgg ggaagaccat tgacttcaac ggccgcaagg
     1561 tcacgtatcc tgaagtcagg cttgtattgt gggcgggcgg caatcccttc gcgcaccagc
     1621 ccgacacg
//
LOCUS       DBMDTI010000379         1623 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_16276_length_1623_cov_3.448752, whole genome shotgun sequence.
ACCESSION   DBMDTI010000379 DBMDTI010000000
VERSION     DBMDTI010000379.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16276_length_1623_cov_3.448752"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1623
                     /locus_tag="ACZPB6_10795"
     CDS             <1..>1623
                     /locus_tag="ACZPB6_10795"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR1184984.1"
                     /translation="AELVELAHAVDEFGIEVAELVDGVSKLDKLRFSSAAAAQAESFR
                     KMLLAMSRDLRVVIVKLADRVHNMRTLGVMRPEKCARIANETLDIYVPIAHQLGMNNV
                     FRELQELSFANRYPLRYKVLYENVLLMRNNRRAVLERILNETKAFLPKQGIRARIIGR
                     DKTIYGIYNKMRENHQSFSDALDIYGFRVIVRSVDDCYLALGALHRLYKPIHRRFKDF
                     IAIPKLNGYQSLHTTVIGPYGTPVEFQIRTEQMHQVAENGILTQWMFKNDFDSSDIQS
                     RTAAWLQSLMEIQRTSQDSSEFIENIKVDLFPNRLYAFTPKGQIISLPRGSTPVDFAY
                     QIHTDVGNHTNGCRINGEDRPLSAQLHNGDMVEIITSPDASPSPEWLDFIHSGRARAE
                     IRQYLRNCRFDEAASLGRDVLERLAQGNHLSLDAIPDTVWQKVQNELSVESREALYAS
                     IGLGKDLPATVIHRLVRLMHDTEKEAFATTALPEIVIRGNEGVAVELSKCCHPIPGDK
                     IVGFSRAGHGLVIHRADCPHVERGRKAEPSRWV"
BASE COUNT          338 a          593 c          421 g          271 t
ORIGIN      
        1 agcggagctt gtcgagcttg ctcacgccgt cgacgagttc ggcatcgagg tcgccgaact
       61 cgtcgacggc gtgagcaagc tcgacaagct ccgcttctcc tccgctgccg cggcacaggc
      121 cgaaagcttc cgcaagatgc tcctcgcgat gtcccgcgac cttcgcgtcg tcatcgtcaa
      181 gctcgccgac cgcgtgcaca acatgcgcac gctcggcgtc atgcgccccg aaaagtgcgc
      241 ccgcatcgcc aacgagacgc tcgacatcta cgtgccgatc gcccaccagc tcggcatgaa
      301 caacgtcttc cgcgagctgc aggagctgag cttcgcaaac cgatatccgc ttcgctacaa
      361 ggtgctctac gagaacgtgc ttttaatgcg caacaaccgc cgcgccgttc tcgaacgcat
      421 tctcaacgaa accaaggcct tcctgcccaa gcagggcatc cgcgcacgca tcatcgggcg
      481 cgacaagacg atctacggca tctacaacaa gatgcgcgag aaccaccaat cgttctcgga
      541 cgccctcgac atctacggct tccgcgtcat cgtgcgctcg gtcgacgact gctaccttgc
      601 gctcggcgcc ctgcaccgac tctacaagcc catccaccgc cgcttcaagg acttcatcgc
      661 cattccgaag ctcaacggtt accagagcct gcacacgacc gtgatcggcc cctacggtac
      721 tcccgtcgaa ttccagatcc gcaccgagca gatgcatcag gtcgccgaaa acggcatcct
      781 cacgcagtgg atgttcaaga acgactttga ctcgagcgac atccagtccc gcaccgccgc
      841 ctggctccag agcctgatgg aaattcagcg cacaagtcag gacagcagcg agttcatcga
      901 gaacatcaag gtcgacctct tccccaacag actctacgcc ttcacgccga agggccagat
      961 catctccctg cccagaggca gcacccccgt cgacttcgcg taccagatcc acacggacgt
     1021 gggcaaccac acgaacggct gtcgcatcaa cggcgaggac cgtccgctct ccgctcagct
     1081 tcacaacggc gacatggtgg agatcatcac gtctcccgac gccagcccga gccccgaatg
     1141 gcttgacttc atccactccg gtcgcgcccg agccgaaatt cgccagtatt tgcgcaactg
     1201 ccgctttgac gaggccgcaa gcctcggccg cgacgtactc gaacgtctgg ctcagggcaa
     1261 ccacctgtct ctcgacgcca ttcccgacac cgtctggcag aaggtccaga acgaactgag
     1321 cgtcgaaagc agggaggccc tttatgcctc catcggcctc ggcaaggacc tgcccgccac
     1381 cgtcattcac cgcctcgttc gcctgatgca cgacacggaa aaagaggcct tcgccaccac
     1441 tgcactgcct gagatcgtca tccgtggcaa tgaaggcgtc gccgtcgagc tctcgaagtg
     1501 ctgtcacccg atccccggcg acaagatcgt gggcttcagc cgcgccggtc acggcctcgt
     1561 catccaccgc gcggactgcc cgcacgtcga gcgcggccgc aaggccgaac cgtcacgctg
     1621 ggt
//
LOCUS       DBMDTI010000380         1604 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_16523_length_1604_cov_2.236860, whole genome shotgun sequence.
ACCESSION   DBMDTI010000380 DBMDTI010000000
VERSION     DBMDTI010000380.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16523_length_1604_cov_2.236860"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="ACZPB6_10800"
     CDS             <1..279
                     /locus_tag="ACZPB6_10800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1184985.1"
                     /translation="AADEGTAVRLTVANTGALLTPEALAEIGRPLASSKSSGLGLGLM
                     ICKSIAEAHLATLAFSARTARDGGGLAVTLTMPARSEAPLHENYGNLP"
     gene            276..917
                     /locus_tag="ACZPB6_10805"
     CDS             276..917
                     /locus_tag="ACZPB6_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1184986.1"
                     /translation="MTESEFDPAERDSALIRLVDDEPDVRAALSLMLEIEGWRCVSYA
                     SAREFLVENMDSPGCLVLDVRMPGMSGLELQQAMEERGIELPVIFLTGHGDLDTAIQS
                     LKRGAVDFLLKPVDDEKLLAAIASAVHKDRLRREGLSAELLKAGAKALSDRERHLLEL
                     ISLGRSDARIASELGISERTVQGHRAKIYRKFGIHSGKVLEAMLPDIMRLIGR"
     gene            1133..>1604
                     /locus_tag="ACZPB6_10810"
     CDS             1133..>1604
                     /locus_tag="ACZPB6_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1184987.1"
                     /translation="MTANLTRRRMLAAAGIGTAAAAFTGAGSAQCRNDVKWDETHEIV
                     VVGAGGAGMAAAVKAAQTGAKDILVLEKLAFTGGNTLIAQGFINAADPVRQPRQGIKD
                     SPENHMKQTLAAGDFRGDPERVKVLCDNAYGAITWLESLGMKFKDNVIQIFGALY"
BASE COUNT          300 a          606 c          466 g          232 t
ORIGIN      
        1 gcggcagatg aaggcaccgc cgtacgcctc accgtcgcca acacgggcgc gcttctcacg
       61 cccgaagcgc tcgccgagat cggacgtccg ctcgcaagct ccaagtcctc gggcctcgga
      121 ctcggactca tgatctgcaa gtcgatcgcc gaggcccacc tcgccacgct cgccttctcg
      181 gcccgcaccg caagggacgg cggcggactc gccgtcacgc tgacgatgcc cgcccgaagc
      241 gaagcgcctc tgcatgaaaa ctacggaaat ctgccatgac cgaatccgaa ttcgatcccg
      301 ccgagcgcga ctcggcactc atccgcctcg tcgacgacga gcccgacgtg cgcgccgcac
      361 tatccctgat gctcgagatc gaaggctggc gatgcgtgag ctacgcgagc gcgcgggagt
      421 tccttgtcga gaatatggac tctcccggct gcctcgtcct cgacgtgcgc atgccgggca
      481 tgagcggcct cgaacttcag caggcgatgg aggagcgcgg gatcgagctg cccgtcatct
      541 tcctcacggg ccacggcgac ctcgacaccg cgatccagag cctcaagcgc ggcgcggtcg
      601 actttctgct caaacccgtc gacgacgaga agctcctcgc cgccatcgcc tcggccgtgc
      661 acaaggaccg cctgcgccgc gaaggcctct cggccgagct cctcaaggcg ggcgccaagg
      721 ccctgagcga ccgggaaaga catctgctcg aactcatctc gctcggccgc agcgacgcgc
      781 gcatcgcctc cgagctcggc atctccgagc gcaccgtgca gggccaccgc gccaaaatct
      841 accggaagtt cggaattcat tcgggcaagg tcctcgaggc catgctgccc gacatcatgc
      901 gcctcatcgg cagatgaggc cggccccgcc gttcttcgcc gccgcccgcc gctctcttct
      961 tcacgcacgg gacgaaatcg cctcccgttg accttttcca agttacgcgc gtaatccgtc
     1021 ggccgacaat gcagtcatga caaaccgagc gcagcccgcg ggcttcgggg tccccagaag
     1081 gggccccgtc gaacaagacc acccgctgct tcgctccaga aggagaaaca tcatgaccgc
     1141 gaatctcacc cgccgccgca tgctcgccgc cgcaggcatc ggcaccgccg cagccgcctt
     1201 cacgggcgcc ggatccgcac agtgccgcaa cgacgtcaaa tgggacgaaa cccacgaaat
     1261 cgtcgtcgtg ggcgcgggcg gcgccggcat ggccgccgcc gtcaaggccg cccagacggg
     1321 cgccaaggac atcctcgtcc tcgagaagct cgccttcacg ggcggcaaca cgctcattgc
     1381 ccagggcttc atcaacgccg ccgaccccgt gcgccagccg cgtcagggca tcaaggactc
     1441 gcccgaaaac cacatgaagc agacgctcgc cgcaggcgac ttccgcggcg atcccgaacg
     1501 cgtcaaggtc ctctgcgaca acgcctacgg cgccatcacc tggctcgagt ccctcggcat
     1561 gaagttcaag gacaacgtca tccagatctt cggcgcgctc tatc
//
LOCUS       DBMDTI010000381         1603 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_16544_length_1603_cov_2.058589, whole genome shotgun sequence.
ACCESSION   DBMDTI010000381 DBMDTI010000000
VERSION     DBMDTI010000381.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16544_length_1603_cov_2.058589"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="ACZPB6_10815"
     CDS             complement(<1..247)
                     /locus_tag="ACZPB6_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR1184988.1"
                     /translation="MKSPYIAVAVKAARRAASEITRAATSLDTLEVENKSVNDFVTAA
                     DRAAEAAIVDCLQHAYPDHAILTEEAGRIGAAKSDYLW"
     gene            550..1347
                     /locus_tag="ACZPB6_10820"
     CDS             550..1347
                     /locus_tag="ACZPB6_10820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIR1184989.1"
                     /translation="MDIPQLASRVRFVLCEPAHPGNIGSAARAIKTMGFRDLRVVAPR
                     EPGYRTHEEAVAYATSSVDVLEASRMHDTLAEALEGVTFAWALTGYDREFGPPMSPLR
                     PAAERSVAWLDEMEGDIAFVFGTERSGMTNEEVMLCQGCAAIAADPASPSLNLAQAVQ
                     ITAYEMHMALMAAEGHEGELYDWQSRFEHEQPASVEAVEGFFRHWEEAMTACGVHDPA
                     NPKFLMPITRRLFGRSGLTQTEIDLLRGICAAIIRPKRERKGSKTGA"
BASE COUNT          276 a          524 c          506 g          297 t
ORIGIN      
        1 tccagaggta gtcgctcttg gcggcgccga tgcggccggc ttcttccgtc agaatggcgt
       61 gatcggggta ggcgtgctgc aggcagtcga cgatggcggc ttcggcggcg cggtcggcgg
      121 cggtcacgaa atcgttgacg gacttgtttt caacttcgag ggtgtcgagg ctcgtggcgg
      181 cgcgggtgat ttccgaggcg gcgcggcggg cggccttgac ggcgacggcg atataaggcg
      241 atttcatacg atgagaaaca gtgtgggatg taaaacgttg cggcgcacga ctggggagca
      301 gagggctcga cccttgtcgg cgcaaacaat tattttagcc gatcaaaggg attgctgagc
      361 aaaaaaacag gcttgaattg agaccttcgg cgaggtgcgc cttcctccgt ctccctctgc
      421 cgcacgggcg ccgcggccga atttaggtag aatgagcgga ttccctgcgg cggatgctcc
      481 ctgcggtccg ccctcccatt ctgcgccacc tcttcatctt gtgccgcagc cgctttcgga
      541 tacatcgaca tggacattcc ccagctcgcc tcccgcgtgc gcttcgtgct ctgcgagcct
      601 gcacatcccg gcaacatcgg ctccgccgcc cgcgccatca agacgatggg ctttcgagac
      661 cttcgcgtcg tcgcaccccg cgagccgggc taccgcactc atgaagaggc ggtcgcctat
      721 gcgacgagct ccgtcgacgt gctcgaggcc tcccgcatgc acgacacgct cgccgaagcg
      781 cttgagggcg tgaccttcgc ctgggcgctt acgggctacg accgagagtt cggtcctccg
      841 atgagccccc tgcgccccgc cgccgagcgt tccgtcgcct ggctcgacga aatggagggc
      901 gacatcgcct tcgtcttcgg caccgagcga agcggcatga cgaacgagga ggtcatgctc
      961 tgtcagggct gcgccgccat cgccgccgat ccggcgagtc cgagcctcaa cctcgctcag
     1021 gccgtccaga tcacggccta cgagatgcac atggcgctca tggccgccga agggcatgag
     1081 ggagagctct acgactggca gtcccgcttc gagcacgagc agcctgcaag cgttgaggcc
     1141 gtcgagggat tcttccgcca ctgggaggag gccatgaccg cctgcggcgt gcacgatccc
     1201 gccaatccca agttcctgat gccgatcacg cgaaggctct tcggacgcag cggcctcacg
     1261 caaaccgaga ttgatctcct gcgcggcatc tgtgcggcga tcatccgtcc caagcgcgag
     1321 cgcaagggct cgaagacggg tgcctgagag gatccgtcct gcgagccccc tcgctttctg
     1381 ccacaataga atcttcggcc cgcgcggcgg gcctcaccta cagagacaca tccatgcttc
     1441 agtacgcacg tgaactcatc gcgaccatca tgcagaagga ccccgcggct cattcccgct
     1501 gggaggtcgt cctcgcctat ccgggctttc acgccgtgat gatccatcgt ctgtcgcact
     1561 ggtgctggac acaggattgg aagacggttg cgcgtgtcgt ctc
//
LOCUS       DBMDTI010000382         1592 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_16688_length_1592_cov_2.191147, whole genome shotgun sequence.
ACCESSION   DBMDTI010000382 DBMDTI010000000
VERSION     DBMDTI010000382.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16688_length_1592_cov_2.191147"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="ACZPB6_10825"
     CDS             complement(<1..488)
                     /locus_tag="ACZPB6_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005432958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TroA family protein"
                     /protein_id="HIR1184990.1"
                     /translation="MTFNLKQCALVCGALALAATSAAARTVVDDNGTSVEIPDHVNRV
                     VVTNILPLASAVTVFLNDGKTVVGMHPASYSAAKSGLLGKLYPDVLKADTSFMQGASL
                     NVEALMALRPDLVLVNAPDKRTLDAVRNAGIPAFGISPSKWHYDIIETHAQWMKSLSE
                     IWP"
     gene            complement(485..>1592)
                     /locus_tag="ACZPB6_10830"
     CDS             complement(485..>1592)
                     /locus_tag="ACZPB6_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012375.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HIR1184991.1"
                     /translation="AGLGGMTVIHDASGCNSTYTTHDEPRWNTMPSAVFISALTEMDA
                     VLGNDSRIIDDIVDAARKLTPAFVAIAGTPIPMMTGVDLKGIARLVESRTRIPSFAVQ
                     TNSMRAYSVGCAQAWIELSRRFVVPNGARAGSFGINLLGATPLDFSTGGMLESLRAAA
                     ARAGYTINACWAMGDKLESLANTAAARVNVVVSSSGLPLAQCFERCFGTPYVCGLPVG
                     GLAPRWHAAVEWAAKNRRSVPAADFLGADAGGTRTAVLGEPLAAACTAAAVNLEQPGS
                     CRALSPLPSLGIEAPILSSNLSEDLLRAMLGGTEVLAADPLFDIIAEEARVGRTIAFP
                     HEACSGRMYRSGIVNILERAPFDALVGNLLDTAK"
BASE COUNT          242 a          462 c          639 g          249 t
ORIGIN      
        1 ggccagatct cggagaggct cttcatccac tgggcgtgcg tctcgatgat gtcgtagtgc
       61 cacttgctcg ggctgatgcc gaaggcggga atgcccgcgt tgcggaccgc gtcgagcgtg
      121 cgcttgtcgg gcgcgttgac gaggacgagg tcgggcctga gggccatcag ggcctcgacg
      181 ttgaggctcg cgccctgcat gaaggaggtg tcggccttga ggacgtcggg atagagcttg
      241 ccgaggagcc cgctcttggc ggccgagtag gaggcgggat gcatgcccac cacggtcttg
      301 ccgtcgttga ggaagacggt cacggcggag gcgagcggaa ggatgttcgt gacgaccacg
      361 cggttgacgt ggtcggggat ctcgacgctg gtgccgttgt cgtcgacgac ggtgcgtgcg
      421 gccgcggagg tcgcggccag ggcgagtgcg ccgcagacga gggcgcattg cttcagattg
      481 aaggtcattt tgcagtgtcc agcagattgc cgacgagtgc gtcgaacggg gcgcgctcga
      541 gaatgttcac gatcccgctc ctgtacatgc ggcccgagca ggcctcgtgc gggaaggcga
      601 tggtgcgccc gacgcgggcc tcctcggcga tgatgtcgaa gagcgggtcg gcggcgagca
      661 cctccgtgcc gccgagcatg gcgcgcagga ggtcctcgga aaggttcgag gagagaatgg
      721 gcgcctcgat gccgagggac ggcaggggcg agagcgccct gcagcttccg ggctgctcga
      781 ggttgacggc cgcggccgtg caggcggcgg caagcggctc tccgagcacg gccgtgcggg
      841 tgccgcccgc atccgcgccg aggaagtcgg cggcgggcac gctgcggcgg ttcttcgcgg
      901 cccattcgac ggccgcgtgc cagcggggag caagtccgcc cacgggcagg ccgcagacgt
      961 agggcgtgcc gaagcagcgc tcgaagcact gcgcgagcgg aagcccggac gagctcacga
     1021 cgacgttgac gcgcgcggcc gcggtgttgg cgagggattc gagcttgtcg cccatcgccc
     1081 agcaggcgtt gatcgtgtag cctgcgcgtg cggcggcggc cctgagggac tcgagcatgc
     1141 cgcctgtcga gaagtcgaga ggcgtcgcgc ccagaaggtt gatgccgaag gaccccgcgc
     1201 gggcgccgtt gggaacgacg aagcggcgcg agagttcgat ccaggcctgt gcgcagccca
     1261 cgctgtaggc gcgcatggag ttggtctgga cggcgaagga cggaatgcgg gtgcggcttt
     1321 cgacgaggcg ggcgatcccc ttgaggtcga cgccagtcat catgggaatg ggcgtgccgg
     1381 cgatggcgac gaaggcgggc gtgagcttgc gggctgcgtc gacgatgtcg tcgatgatgc
     1441 ggctgtcgtt gccgagcacg gcgtccatct cggtgagcgc cgagatgaag acggccgagg
     1501 gcatcgtgtt ccagcggggc tcgtcgtgcg tggtgtaggt cgagttgcag cccgaggcgt
     1561 cgtggatgac ggtcatgccg ccgagccccg ct
//
LOCUS       DBMDTI010000383         1584 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_16798_length_1584_cov_2.821982, whole genome shotgun sequence.
ACCESSION   DBMDTI010000383 DBMDTI010000000
VERSION     DBMDTI010000383.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16798_length_1584_cov_2.821982"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            69..>1584
                     /gene="dxs"
                     /locus_tag="ACZPB6_10835"
     CDS             69..>1584
                     /gene="dxs"
                     /locus_tag="ACZPB6_10835"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR1184992.1"
                     /translation="MTQQTLLDSISSPADLRRLDVHQLPQLAQELRAFMVESVSKTGG
                     HLSSSLGATELAIAIHTAFNTPEDRVIWDVGHQAYAHKILTGRREGMATLRKHHGLSG
                     FPKRTESPYDAFGTAHSSTSISAALGMAIAARLEDKTDRWHIAVIGDGALTGGMALEA
                     LNDAGVWKEGVRLLVILNDNDCSISPPAGALSNHLAKIVSTRAYTCAREISKRVLKPV
                     PGLWDIAKRMEKQAINFVSPPSGIFSSFDLNYYGPVDGHDVVGLVEVLKNLRRLNCPC
                     VLHVATQKGKGYEPAEMDPTAYHGVGVFDPLLGLPEKKPGRPTYTEVFGRWLCDTAAA
                     DKRLYGITPAMREGSGLVEFARRYPERYRDVAIAEQHAVTYAAGLACEGIKPVVAIYS
                     TFLQRAIDQVIHDVALQNLPVVFAIDRGGLVGADGATHHGFFDISLLRTIPNMTVMTP
                     SDENECRLMLNTAYSMNTPASVRYPRGRGPGVEVTAGGGTIPVGRSRTLRKGSRV"
BASE COUNT          279 a          566 c          472 g          267 t
ORIGIN      
        1 agatcgccga ctacgtgatc gcgcgctcgc actaaaatga cccaacttca catataggcc
       61 gccctcaagt gactcagcaa acgcttctcg actccatcag ctcgcctgcg gacctgcgca
      121 ggctcgacgt gcaccagctc ccgcagctcg ctcaggagct ccgcgcgttc atggtcgagt
      181 ccgtctcgaa gacgggcggc catctttcga gctcgcttgg cgcgacggag ctcgccatcg
      241 cgattcacac ggcgttcaac acgcccgagg accgcgtcat ctgggacgtc ggccatcagg
      301 cctacgccca caagatcctg acgggacgcc gtgagggcat ggccacgctg agaaagcacc
      361 acggcctctc gggcttcccg aagcgcacgg agtctcccta cgacgccttt ggcaccgcgc
      421 actcctccac ctcgatctcg gccgcactcg gcatggccat tgcggcccgc cttgaggaca
      481 agaccgatcg ctggcacatc gctgtgatcg gcgacggcgc gctgacgggc ggcatggcgc
      541 tcgaagccct gaacgatgcg ggcgtctgga aggaaggcgt caggcttctc gtcatcctca
      601 acgacaacga ctgctcgatc tcgccgcctg cgggcgcgct ctcgaatcat ctcgcgaaga
      661 tcgtctcgac gcgcgcctac acgtgcgcgc gcgaaatctc caagcgcgtc ctcaagcccg
      721 tgcccggact ctgggacatc gccaagcgta tggagaagca ggcgatcaac ttcgtgagtc
      781 cgccctcggg catcttctcg agcttcgacc tcaactacta cggtcccgtc gacggccacg
      841 acgtcgtcgg actcgtcgag gtgctcaaga acctgcgcag gctcaactgc ccgtgcgtgc
      901 tgcacgtcgc gacccagaag ggcaagggct acgagcctgc tgaaatggat ccgacggcct
      961 accacggcgt gggcgtcttt gatccgcttc tcggccttcc cgaaaagaag ccgggcagac
     1021 cgacctacac cgaagtcttc gggcgctggc tctgcgacac ggccgccgcc gacaagcgcc
     1081 tctacggcat aacgcccgcc atgcgcgagg gctcgggact cgtcgagttc gcgcgccgct
     1141 atcccgagcg ctatcgcgac gtcgccatcg ccgagcagca tgcggtcacc tatgctgcag
     1201 gccttgcctg cgagggcatc aagcccgtcg tggcgatcta ctcgactttc ctgcagcgtg
     1261 cgatcgacca ggtcattcat gacgtggcgc tccagaacct gcccgtcgtt ttcgcaatcg
     1321 accgaggcgg tctggtgggc gccgacggcg cgacgcacca cggcttcttc gacatttcgc
     1381 ttcttcgcac gattccgaac atgacggtga tgacgccctc cgacgagaac gagtgccgcc
     1441 tcatgctcaa cacggcctac tcgatgaaca cgcccgcctc cgtccgctat ccgcgcggcc
     1501 gcggcccagg cgtcgaggtg acggccgggg gcgggacgat tcccgtcggc cgctcgcgca
     1561 ccctgcgaaa gggcagtcgc gtgg
//
LOCUS       DBMDTI010000384         1583 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_16815_length_1583_cov_2.114710, whole genome shotgun sequence.
ACCESSION   DBMDTI010000384 DBMDTI010000000
VERSION     DBMDTI010000384.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16815_length_1583_cov_2.114710"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="ACZPB6_10840"
     CDS             complement(<1..240)
                     /locus_tag="ACZPB6_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR1184993.1"
                     /translation="MQADCPHGRLVVGAGGAGMVAAATAVDNGATVIVLEKMVMLGGN
                     TARSEGNMSAMDPEPEKLLPMTQAVRDIIAKYTNPK"
     gene            complement(248..>1583)
                     /locus_tag="ACZPB6_10845"
     CDS             complement(248..>1583)
                     /locus_tag="ACZPB6_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR1184994.1"
                     /translation="HAEGEAGTRSAELVKRARRQHGIEAVRTIIRFAMDRMRETQLQE
                     VASSMTLTTLLSLVPLIAVAMSAFALFPSFADSRHALEEAIISGVLPQQYTETIVTYL
                     RNFTDHASGLGIFGLAGLAVTALLLIDKFFVTVNRIYQVRRMRPWGQRALLYWALITL
                     GPLMIAFSLTVSTQAIRSASEGVDATSVEWGMAVLQFAIQVASYTFLYKFVPNCHVQL
                     SHAAVGGVSASFAGLAVREAFGHYVSAGTLTSIYGAFVAFPVLLLWFYVSWLIVFGGA
                     AITATIPLLTSGRFADSYKRGNDFLTGVALLKTLWRHRREGETSVGITTLADEVDSYP
                     QAARRILLRLAEKGYCVEIMSEKPKSHSDWALLCDPSAKSLRDACTALLVDGTNHLTS
                     PKRASSSRDEGMLFDWYRGFMSAEMLAKPLDEVFTIDEEPQDVQAEQTARPA"
BASE COUNT          288 a          495 c          548 g          252 t
ORIGIN      
        1 cttcgggttc gtgtacttgg cgatgatgtc gcgcacggcc tgcgtcatcg gcagaagctt
       61 ttcgggttcg gggtccatgg cggacatgtt gccttcggag cgggcggtgt tgccgccgag
      121 catgaccatc ttctcaagga cgatcacggt cgcgccgttg tcgacggccg tcgcggcggc
      181 aaccatgccg gcgccgcccg caccgacgac gagccgccca tgagggcagt ctgcctgcag
      241 gtccccgtca ggcgggacgg gcggtctgtt ccgcctgaac gtcctgcggc tcctcgtcga
      301 tggtgaagac ctcgtctagg ggcttcgcga gcatttcggc ggacatgaag ccgcggtacc
      361 agtcaaagag catcccttca tctcgggacg acgaggcgcg cttgggacta gtgagatggt
      421 tggtgccgtc gacgagaagc gccgtgcagg cgtccctgag gctcttggcg gacggatcgc
      481 acagaagcgc ccagtcgctg tgactcttgg gcttctcgct catgatctcg acgcagtaac
      541 ccttttcggc gagcctgagc agaatgcggc gggcggcctg cgggtagctg tcgacctcgt
      601 cggcgagcgt cgtgatgccc accgaggtct cgccctcgcg gcgatggcgc cagagcgtct
      661 tcaggagcgc gacgcccgta aggaagtcat tgccgcgctt gtagctgtcg gcgaagcgcc
      721 ccgaagtgag aagcgggatc gtcgccgtga tggctgcgcc gccgaagacg atgagccagg
      781 agacgtagaa ccagagcagg agcacgggga aggccacgaa cgcgccgtag atcgacgtga
      841 gcgtgcccgc cgagacgtag tgcccgaagg cctcgcgcac ggcgagtcct gcgaaggaag
      901 cggagacgcc gcccacggcc gcgtgcgaaa gctgcacgtg gcagttgggc acgaacttgt
      961 agaggaaggt gtaggaggcg acctggatcg caaactgcag aacggccatg ccccactcga
     1021 ccgacgtcgc gtcaacgccc tccgaggcgg agcggatcgc ctgcgtcgag acggtgagcg
     1081 agaaggcgat catcaggggc ccgagcgtga tgagcgccca gtagagcagc gccctctgtc
     1141 cccaggggcg catgcggcgc acctgataga tgcggttgac cgtcacgaag aacttgtcaa
     1201 tcaggagaag cgccgtcacg gccagacccg ccagaccgaa gatgccgagt cccgaggcgt
     1261 ggtccgtgaa gttgcgcaga tacgtgacga tcgtctccgt gtactgctgc ggcagcacgc
     1321 ccgagatgat cgcctcctca agcgcgtggc gcgagtccgc gaagctcggg aagagcgcaa
     1381 aggccgacat cgcgaccgcg atgagcggaa cgagcgaaag gagcgtcgtg agcgtcatcg
     1441 aggacgccac ctcctgaagc tgagtctcgc gcatgcggtc catcgcaaag cggatgatcg
     1501 tgcgcacggc ctcgatgccg tgctgccgcc gagcccgctt cacaagctcc gcactcctcg
     1561 ttccggcctc tccctctgca tgg
//
LOCUS       DBMDTI010000385         1547 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_17350_length_1547_cov_1.792531, whole genome shotgun sequence.
ACCESSION   DBMDTI010000385 DBMDTI010000000
VERSION     DBMDTI010000385.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17350_length_1547_cov_1.792531"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="ACZPB6_10850"
     CDS             complement(<1..919)
                     /locus_tag="ACZPB6_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012493.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR1184995.1"
                     /translation="MMRRLKTDKSIIDDLPEKVSIDEYCTLTKEQVALYNEVVKRSMR
                     LVEASKDTFMRHSLVLQMIMQLKQICNSPALFEPAGGFEDPRLSGKMCRLFDILEEMP
                     AAGRKVLVFTQFAQMGMLLQEWIANETGRRPQFIHGGLKPNARQTMVDRFQNRRDENV
                     MVLTLKAAGSGLNLTAASVVIHYDLWWNAAVETQATDRAYRIGQQSTVNVYRFISANT
                     FEEKINQIRIYSGTKYELINEADAGMVCAVTGLDHTYPGQGFGIEAQGETPVLEPVLH
                     FRLILPEGVEPAVMLPKLRQIEEEEPELHI"
     gene            complement(916..>1547)
                     /locus_tag="ACZPB6_10855"
     CDS             complement(916..>1547)
                     /locus_tag="ACZPB6_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012403.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HIR1184996.1"
                     /translation="QATLRPYQVRGYSWLMRNLRADMGAIIADDMGLGKTLQVITVLE
                     KLRLDGELDVKQVLVVVPTTLLANWKREIARFAPNLTVSLFYGPKRDLAEATGDVVLT
                     TYGSLRGSMKALEHKHFRLVVIDEAQAIKNYKTAAFRHVRELKTEGFIAMSGTPVEND
                     LMEFWSIMEASNPGILGSSTSFRQDYVYPIENDHDAVTADRFKRITAPS"
BASE COUNT          309 a          450 c          445 g          343 t
ORIGIN      
        1 caatatgtaa ttccggctct tcctcctcga tctggcgcaa ttttggcagc atgactgccg
       61 gctccacgcc ctccgggaga atcagacgga aatgcagtac cggctctaat accggtgttt
      121 ctccctgcgc ttctattcca aatccctgtc ccggatatgt gtggtctaat ccggtcacgg
      181 cgcacaccat tccggcatcc gcttcattga tcagctcata tttggttccg gaataaatgc
      241 ggatctggtt gatcttctcc tcgaaggtat tggccgaaat gaagcggtag acattgacgg
      301 tcgactgttg tccgatgcgg taggcgcggt ccgtggcctg agtttcgacg gcggcgttcc
      361 accagaggtc atagtgaatg acgacggacg cagccgtcag gttgagaccc gaacccgccg
      421 ccttgagcgt aagcaccatg acgttttcat cgcgtcgatt ctggaatcgg tccaccattg
      481 tctggcgcgc attcggcttg aggccgccgt ggatgaactg cgggcgacga cccgtttcgt
      541 tggcaatcca ttcctgaagg agcatgccca tctgtgcaaa ctgcgtgaag acgagcacct
      601 tgcggccggc ggccggcatt tcctcaagaa tgtcgaagag gcggcacatt ttgccggaaa
      661 gcctcggatc ctcgaagccg cccgcaggtt cgaagagcgc aggcgaattg cagatctgct
      721 tgagctgcat gatcatctgc agcaccagcg aatggcgcat gaaggtgtcc ttgctagcct
      781 ccacaagacg catggaacgc ttgaccacct cgttgtagag cgcaacctgc tccttcgtga
      841 gcgtgcagta ctcatcgatg gaaacctttt cgggcagatc gtcgatgatg gacttgtcgg
      901 tcttgaggcg gcgcatcatg aaggggccgt tatgcgcttg aaccgatcgg cggtgacggc
      961 atcgtggtcg ttttcgatcg gatacacata gtcctgcctg aaggatgtgc tcgaaccaag
     1021 aatgcccggg ttcgacgcct ccatgatcga ccagaattcc atcagatcgt tctcgacggg
     1081 cgtgcccgac atggcgatga agccttctgt cttgagctcg cggacatgcc tgaaagcggc
     1141 cgtcttgtaa ttcttgatcg cctgcgcttc gtcaatgaca acaaggcgga aatgcttgtg
     1201 ctcgagcgcc ttcatcgagc cgcgaaggct gccgtaggtc gtgagcacaa cgtcccccgt
     1261 cgcctccgcc agatcacgct tggggccgta gaagagcgat accgtcaagt tcggtgcaaa
     1321 cctcgcgatc tcacgcttcc agtttgcgag cagcgtggtc ggcacgacga cgagcacctg
     1381 cttgacatcc aactcgccgt cgaggcgcag cttttcgagc accgtgatga cttgaagcgt
     1441 cttgcctagg cccatgtcgt cggcgatgat ggcccccatg tccgcgcgca gattgcgcat
     1501 gagccaggaa tagcctcgca cctgataggg acgcaacgtc gcctgga
//
LOCUS       DBMDTI010000386         1546 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_17360_length_1546_cov_2.175779, whole genome shotgun sequence.
ACCESSION   DBMDTI010000386 DBMDTI010000000
VERSION     DBMDTI010000386.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17360_length_1546_cov_2.175779"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="ACZPB6_10860"
     CDS             <1..1542
                     /locus_tag="ACZPB6_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HIR1184997.1"
                     /translation="IDPHHSHLGDINCTSCHTAHARPKLVCNDCHTFPNKMPFADAPK
                     AAPKVSPDEAAIEAAMKAEPRESYDVVVIGAGSAGFNAAIAARRAGASVLLLEKHSFS
                     GGNSMLAAGGYNAVGTKQQKAKGINDTVEAYVEDTLKGGRGKNDRALVEILAKESAAG
                     VEWLEGMGADLSEVKRSGGARVDRTHRPTGGETVGPHIVKTLRAQAEKDGVPARLNSR
                     AVRLIVDENHTVRGVVVEGKHSGLYRVNAKAVVLAAGGYGQNKEMVAFYRPTFKGMTS
                     SNNVTSTGDGVRMALEAGASMTDIDWIQAHPTVGKGSRILISETVRGVGAVMVNETGA
                     RFVNELTTRDRASDAILKQPGRHAWLVFDADLYAKAKMVRGYDHLGMLKKADTIEGLA
                     KAAGMDPKVLEKTLADYNAYRAKGHDDAFGRPDMPLAVAKAPFYAVEVAPGIHHTMGG
                     VAVTTESEVLDIQSRPMPGLYAAGEVTGGVHGFNRLGGNAVADTVVFGRRAGEHAAKY
                     ALEQK"
BASE COUNT          308 a          545 c          484 g          209 t
ORIGIN      
        1 atcgatccgc accactcgca tctcggcgac atcaactgca cgagctgcca caccgcgcac
       61 gcccgtccga agctcgtctg caacgactgc cacaccttcc cgaacaagat gccgttcgcc
      121 gatgcgccca aagccgcacc gaaggtctcc cccgacgaag cggccatcga ggcggcaatg
      181 aaggccgagc cgcgcgaatc ctatgacgtc gtggtgatcg gcgcgggcag cgcgggcttc
      241 aacgccgcga tcgccgccag gcgtgcgggc gcctccgttc tccttctcga aaagcacagc
      301 ttctcgggcg gcaactccat gctcgccgcg ggcggctaca acgccgtcgg gacgaagcaa
      361 cagaaggcca agggcatcaa cgacacggtc gaagcctatg tcgaggacac gctcaagggc
      421 ggccgcggca agaacgaccg agccctcgtt gaaatcctcg cgaaggaaag tgccgcaggt
      481 gtcgaatggc tcgagggcat gggcgcggac ctctccgaag tgaagcgctc gggcggcgcc
      541 cgcgtcgacc gcacgcatcg cccgacgggc ggcgagacgg tcggtccgca catcgtcaag
      601 actctgcgcg ctcaggccga gaaggacggc gtgcccgcac gcctcaactc ccgcgccgtc
      661 aggctcattg ttgacgaaaa ccacaccgtc aggggcgtcg tggtcgaagg caagcacagc
      721 ggcctctacc gcgtcaacgc caaagcggtc gtgctcgctg ctggcggcta cggccagaac
      781 aaggagatgg tggccttcta tcgtccgacc ttcaagggca tgacgagctc gaacaacgtc
      841 acgagcaccg gcgacggcgt gcgcatggca cttgaagccg gcgcgagcat gaccgacatc
      901 gactggatcc aggcccaccc gaccgtcggc aagggaagcc gcattctcat cagtgaaacg
      961 gtccgcggcg tcggcgccgt catggtcaac gaaacgggcg cgcgcttcgt caacgaactc
     1021 acgacccgcg accgcgcctc cgacgcgatc ctcaagcagc ccggccgcca cgcctggctc
     1081 gtcttcgacg ccgatctcta cgccaaggcc aagatggtcc gcggctacga ccacctcggc
     1141 atgctgaaga aggccgacac gatcgaaggg cttgccaagg ctgccggcat ggatccgaag
     1201 gtccttgaaa agacgctcgc cgactacaac gcgtaccgcg ccaagggcca tgacgacgcg
     1261 ttcgggcgtc ccgacatgcc gctcgccgtc gcgaaggcgc ccttctacgc cgtcgaagtt
     1321 gcgcccggca tccatcacac gatgggcggc gtggccgtca cgaccgaaag cgaggtcctc
     1381 gacatccagt cccgtccgat gcccggcctc tatgccgcag gtgaagtgac gggcggcgtg
     1441 cacggcttca accgcctcgg cggcaacgcc gtcgccgaca ccgtggtctt cggtcgccgt
     1501 gcaggcgaac acgctgcgaa gtacgcgctc gaacagaagt gagcgc
//
LOCUS       DBMDTI010000387         1528 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_17625_length_1528_cov_1.426069, whole genome shotgun sequence.
ACCESSION   DBMDTI010000387 DBMDTI010000000
VERSION     DBMDTI010000387.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17625_length_1528_cov_1.426069"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            19..567
                     /locus_tag="ACZPB6_10865"
                     /pseudo
     CDS             19..567
                     /locus_tag="ACZPB6_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            937..1362
                     /locus_tag="ACZPB6_10870"
     CDS             937..1362
                     /locus_tag="ACZPB6_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013717.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR1184998.1"
                     /translation="MACKSRYRMHSQLDGTVSHDFANIEDREPLWIHPDDAAARGIRN
                     GDIVLVSNKRGAALAGAYVTERILKGVVVLHHGAWFDPQDVKGRRIDVHGNSNTLTMD
                     EPTSKLACGNIASTANVEVPKWTGELPDVSVYREPRIVR"
BASE COUNT          320 a          514 c          438 g          256 t
ORIGIN      
        1 tagtacggcg aacagcctca ctggatggcc tttgcgctcg cgagcgttct gggccagatc
       61 ggcctgccgg gcggcggcat cggcacgaac taccagtatt cgaacggtgg cgctccgatg
      121 gcaaaggggc cgttccttgc gggcgttccc gccaaggtga agcctgcact tcccttcaac
      181 gaaaactgga agggttcaac cacgattccg gtcacgcgct tcgtcgaatg cttcctcaat
      241 ccgggcaaga ccatcgactt caacggccgc aaggtcacgt atcctgaagt caagcttgtg
      301 atgtgggcgg gcggcaatcc cttcgcgcac cagcccgaca cgatgaagct cgagcgtgcc
      361 tggaaggagc ccgagacggt cgtcgtcacc gacacggtct ggaccgccac ggcccgccac
      421 gccgacatcg tgcttcccgc ctgcacgacc ttcgagcaca acgacatcac gagcatcggc
      481 acctacacga acgacggctt cgtcgccatg aagcaggcca tcgaaccgcg cttcgagtcc
      541 cgctccgact actggatctt caccgagctc gccaagcgtc tcggcgtcga gtcccagtac
      601 acggaaggac tcgacgaaat gggctggatc cgccgcctct acgaaggcgc catgaagatg
      661 ggcagggccg gcaagatcgc catgcccgac ttcgacgaat tctggaagaa gggctacgtc
      721 atgttcgacg tcgccgacaa ggaccgcaac tacgtcgcct tcgaggcctt ccgcaaggac
      781 ccgaaggcca atgcactctc gaccgaaagc ggtctcatcc agctctactc gccgaagatc
      841 gcgagctacg gctatgacga ctgcaagggg catccgtgct tcttcgagcc gaccgaaggc
      901 gtctccagag ccaccaagga cgcgcctctc gccctcatgg cctgcaagag ccgctaccgc
      961 atgcacagcc agctcgacgg cacggtgagc cacgacttcg ccaacatcga ggaccgcgag
     1021 ccgctctgga tccatcccga tgacgccgcc gcacgcggca tcaggaacgg cgacatcgtg
     1081 ctcgtctcca acaagcgcgg cgcagcgctt gcgggcgcct atgtgacgga gcgcatcctc
     1141 aagggcgtcg tcgtcctcca ccacggcgcc tggttcgatc cgcaggacgt caagggccgc
     1201 cgcatcgacg tgcacggcaa ctccaacacg ctcacgatgg acgagcccac ctcgaagctc
     1261 gcttgcggca acatcgcctc gacggccaac gtcgaagtgc ccaagtggac gggcgagctg
     1321 cccgacgtca gcgtctaccg cgaaccgcgc atcgtgcgtt gatgcttcga cttgactgac
     1381 gcatgagaaa aaacgccgga ttctccttcg ggggatccgg cgctttattt tggttcatct
     1441 cggaagtccg tggaacgcgg ccttgacttt taagaaaaag tctggtcggc tccctccaag
     1501 aaaaaaaagt agcgctcact ccaaattt
//
LOCUS       DBMDTI010000388         1523 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_17696_length_1523_cov_3.854430, whole genome shotgun sequence.
ACCESSION   DBMDTI010000388 DBMDTI010000000
VERSION     DBMDTI010000388.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17696_length_1523_cov_3.854430"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="ACZPB6_10875"
     CDS             <1..324
                     /locus_tag="ACZPB6_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HIR1184999.1"
                     /translation="EGSMSVKTKATIHWLDAKTAVPAEIRVYDRLFNVPHPDEGEGSF
                     LDRLTPDSKTVLSSYVEPAAAAAARDDKFQFERNGYFVADREDHTAEKPVFNLAVGLK
                     DSWGK"
     gene            415..1509
                     /locus_tag="ACZPB6_10880"
     CDS             415..1509
                     /locus_tag="ACZPB6_10880"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR1185000.1"
                     /translation="MKTKRFKTSLTAVLLALGLSSATPILSAEGGVAEAPQTAEPREY
                     ALERHTLHMGTLINAKLFGATPEAVKHYAIVFEDKVTDYDDLMSVHKDTPLNEVNRRA
                     GEPVEVPCEIADMTKQALAIADETNGAFEPMIGPVVNLWKIGFGGEHVPSDEAIAEAV
                     SKVDRSKVRVWREGGRCMMQIAKGQSIDMGAIAKGFIGQRLSDILKHEGMTRGVLDLG
                     GNIVAVGEKSDGRPWRIGIQHPAESRGGYFAVASVKDESVITSGAYERYFEENGKRYS
                     HILDPKTGRPAKTDISSVTIIDRDGARADALCTALFAMGWDRAEAFLRTHPELKALVM
                     HADMKQAVMTPAAASSVTSADPSVKLTVLK"
BASE COUNT          295 a          443 c          466 g          319 t
ORIGIN      
        1 gagggctcca tgtccgtcaa gacgaaggcg acgatccact ggcttgatgc aaagacggct
       61 gttcctgccg aaatccgcgt ctatgaccga ctcttcaacg tgccacatcc ggacgaaggc
      121 gagggcagct tccttgaccg tctcacgccc gattcgaaga cggtgctttc gtcctacgtc
      181 gagcctgcag ccgctgcggc cgcccgtgat gacaagttcc agttcgagcg caacggctat
      241 ttcgtggccg atcgtgagga tcacacggct gaaaagcctg tcttcaatct ggccgtcggc
      301 ctgaaggatt cctggggcaa gtaatgccct gaatcgtcgg attgccgaag aggggcgggg
      361 ctgatgctcc gccccttgtt gtctcggcac ctcgtgtttt gttctgttga catcatgaaa
      421 accaaacgtt tcaaaacatc tctgactgcc gttctgctgg ctctggggct ttcatccgct
      481 acgccgattc tttcggctga aggaggggtc gccgaggctc ctcagactgc ggagccccgg
      541 gaatacgctc ttgagcgtca tacgcttcac atgggcactc tcatcaacgc gaaactcttc
      601 ggtgctacgc cggaggctgt caagcattac gcgatcgtct tcgaggacaa ggtgacggac
      661 tatgacgatc tgatgtcggt ccacaaggat actcctctca atgaggtcaa tcgccgcgct
      721 ggtgagcccg tcgaagtgcc ttgtgaaatc gccgacatga cgaagcaggc gcttgccatt
      781 gccgatgaga cgaacggcgc cttcgagccg atgataggcc ctgtcgtaaa tctctggaag
      841 atcggcttcg gcggcgagca cgtgccgtcc gatgaggcca ttgccgaggc ggtctccaag
      901 gtcgaccgct ccaaggttcg cgtctggcgc gagggcgggc gatgcatgat gcagatcgcc
      961 aagggacaga gcatcgacat gggtgccatt gcgaagggct tcatcggcca gagactctcc
     1021 gacattctca agcacgaagg catgacgcgt ggtgttctcg atcttggcgg caacatcgtt
     1081 gccgtgggcg agaagtcgga tggccgccct tggcgcatcg gcatccagca tcccgcggag
     1141 tcccgcggcg gttattttgc cgtggcctcg gtgaaggatg aaagcgtgat cacgtcaggc
     1201 gcctacgaac gctacttcga ggagaacggc aagcgctact cccatattct ggatccgaag
     1261 acggggcggc ctgcgaagac tgacatttcg tccgtgacga tcattgatcg cgacggcgca
     1321 cgagcggacg cgctttgcac ggctctcttt gcgatgggct gggatcgtgc ggaggctttc
     1381 ctgcgcaccc atcccgagct gaaggcgctg gtgatgcacg ctgacatgaa gcaggcggtg
     1441 atgacgcctg cggcggcttc gtccgtgacg tccgccgatc cctcggttaa actcaccgtt
     1501 ttgaagtaac cctgcagagc att
//
LOCUS       DBMDTI010000389         1504 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Sutterella wadsworthensis isolate MAG1286
            NODE_17990_length_1504_cov_2.330720, whole genome shotgun sequence.
ACCESSION   DBMDTI010000389 DBMDTI010000000
VERSION     DBMDTI010000389.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508385
            Sequence Read Archive: ERR10114004
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sutterella wadsworthensis (human gut metagenome)
  ORGANISM  Sutterella wadsworthensis
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Sutterellaceae; Sutterella.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 2.6626x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:51:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,178
            CDSs (total)                      :: 2,126
            Genes (coding)                    :: 2,108
            CDSs (with protein)               :: 2,108
            Genes (RNA)                       :: 52
            rRNAs                             :: 3 (5S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 4 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            Pseudo Genes (multiple problems)  :: 1 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Sutterella wadsworthensis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17990_length_1504_cov_2.330720"
                     /isolate="MAG1286"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:40545"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="ACZPB6_10885"
     CDS             complement(<1..1102)
                     /locus_tag="ACZPB6_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024944.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1185001.1"
                     /translation="MTDLALFPELMPEEQFRAVRLQVHNWGTFSGCNDIPIAEEGFLF
                     VGASGSGKSTLLDAFSQLLIPPRWVDFNAAAHETGGKVKGDRSIVSYVRGAWSELQTD
                     AGVTTQYLREETTASGLALTFRNARRIVTLLQVFWIRGKLNGVGDVKRLYAVVERDVD
                     LKALITGFSESDYDLRRLKIDLGDARCSDLFPPYETAFTALLGIKSSLALKLLHKTQS
                     AKNLGDLNEFLRNFMLDEPRTYAIAARLVDEFVELEASHRAVVAANAQIRVLSPAQTA
                     FGDMQQAEEGRLRAKLLAEALPAYRESWRANLLTAQAAKLENERRTHEENVRTIQADL
                     VARRSTLADLELRERQSGGDQIAGWEAEKARRE"
     gene            complement(1099..>1504)
                     /locus_tag="ACZPB6_10890"
     CDS             complement(1099..>1504)
                     /locus_tag="ACZPB6_10890"
                     /inference="COORDINATES: protein motif:HMM:NF025205.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HIR1185002.1"
                     /translation="NYIETYLFLFLRLRLSSAEGRGERAVIGLDEIREHLADIAGRNR
                     DAVGFERHLAGAVTKADHFGVLRRLKSGEDAFEISPVLKMLMTADLVEQVKGYYEGRA
                     AKPGEVAAEIPAGIENDESEDEDDEDDAEDME"
BASE COUNT          236 a          464 c          528 g          276 t
ORIGIN      
        1 gttcgcgacg ggccttttcg gcctcccagc cggcgatctg gtcgccgccg ctctggcgct
       61 cgcgaagctc aaggtcggcg agcgtgctgc gccttgcgac gagatcggcc tgaatcgtgc
      121 ggacgttctc ctcatgcgtg cggcgctcgt tctcgagctt cgcggcctgg gcggtcagaa
      181 ggttcgcgcg ccaggactcg cggtaggcgg ggagcgcctc cgcgaggagc ttcgcgcgga
      241 ggcggccctc ttctgcctgc tgcatgtcgc cgaacgccgt ctgcgcgggc gagagcacgc
      301 ggatctgggc gttggcggcc acgacggcgc ggtgcgaggc ctcgagctcg acaaactcgt
      361 cgacgagacg ggcggcgatc gcgtaggtgc gcggctcatc gagcatgaag ttcctcagaa
      421 attcgttgag gtcgccaagg ttcttcgcgg actgcgtctt gtgaaggagc ttgagcgcaa
      481 ggctgctctt gatgccgagg agcgccgtga aggcggtctc gtagggcgga aagaggtccg
      541 agcacctggc gtcgcccaga tcgatcttga ggcgcctgag atcgtagtcg gactccgaaa
      601 agccggtgat gagggccttg aggtcgacgt cccgctcgac gacggcgtag aggcgcttga
      661 cgtcgcccac gccgttgagc tttccgcgga tccagaatac ctgcaggagc gtcacgatgc
      721 ggcgggcgtt gcggaaggtg agcgcaaggc ccgaggcggt cgtctcctcg cgcaggtact
      781 gcgttgtcac gcccgcatcg gtctgaagct cggaccatgc gccgcggacg taggagacga
      841 tcgagcggtc gcccttgacc ttgccgcccg tctcatgcgc ggcggcgttg aagtccaccc
      901 agcggggcgg aatcagcagc tgggagaagg cgtcgagaag cgtcgacttg cccgatcccg
      961 aggcgcccac gaagagaaag ccctcttcgg cgatcggaat gtcgttgcag cccgagaagg
     1021 tgccccagtt gtgtacctgc aggcgcacgg ccctgaactg ttcttcgggc atgagctcgg
     1081 ggaaaagcgc gagatcggtc attccatgtc ctccgcatcg tcttcgtcgt cttcgtcttc
     1141 ggattcgtcg ttttcaatgc cggcgggaat ttcggcggcg acctcgcccg gcttggcggc
     1201 cctgccctcg tagtagccct tgacctgctc gacgaggtcg gccgtcatga gcatcttgag
     1261 gacgggcgag atctcgaagg cgtcctcgcc gctcttgagg cggcgcagca cgccgaagtg
     1321 gtccgccttg gtgacggcac ccgccagatg cctctcgaag ccgacggcgt cgcgattgcg
     1381 gcccgcgatg tcggctagat gctcgcggat ttcgtcaaga ccgatgacgg cgcgctcgcc
     1441 cctgccctcg gcgcttgaga gcctcaggcg caggaagagg aaaagatagg tctcgatgta
     1501 gttg
//
